bluuebunny/crossref_metadata_2025_split huggingface dataset:
Non-canonical localization of RubisCO under high light conditions in the toxic cyanobacteriumMicrocystis aeruginosaPCC7806
Authors: Barchewitz Tino , Guljamow Arthur , Meissner Sven , Timm Stefan , Henneberg Manja , Baumann Otto , Hagemann Martin , Dittmann Elke 
Published: July 2019

AbstractThe frequent production of the hepatotoxin microcystin and its impact on the life-style of bloom-forming cyanobacteria are poorly understood. Here we report that microcystin interferes with the assembly and the subcellular localization of RubisCO, inMicrocystis aeruginosaPCC7806. Immunofluorescence, electron microscopic and cellular fractionation studies revealed a pronounced heterogeneity in the subcellular localization of RubisCO. At high cell density, RubisCO particles are largely separate from carboxysomes inM. aeruginosaand relocate to the cytoplasmic membrane under high-light conditions. We hypothesize that the binding of microcystin to RubisCO promotes its membrane association and enables an extreme versatility of the enzyme. Steady-state levels of the RubisCO CO2fixation product 3-phosphoglycerate are significantly higher in the microcystin-producing wild type. We also detected noticeable amounts of the RubisCO oxygenase reaction product secreted into the medium that may support the mutual interaction ofM. aeruginosawith its heterotrophic microbial community.
Using a lentiviral Tet-regulated miR-E shRNA dual color vector to evaluate gene function in human leukemic stem cells in vivo
Authors: Maat Henny , Jaques Jennifer , Vellenga Edo , Huls Gerwin , van den Boom Vincent , Schuringa Jan Jacob 
Published: July 2019

AbstractRNA interference is a powerful tool to study loss-of-gene function in leukemic cells. Still, in order to identify effective novel treatment strategies to target and eradicate leukemic stem cells (LSCs), it is critically important to study gene function in a well-controlled and time-dependent manner. We implemented a lentiviral Tet-regulated miR-E shRNA dual color vector in our in vitro and in vivo human leukemia models. Thus, we were able to efficiently introduce doxycycline-inducible and reversible gene repression and trace and isolate transduced miR-E shRNA expressing cells over time. As proof of concept we focused on the non-canonical PRC1.1 Polycomb complex, which we previously identified to be essential for LSCs (1). Here, we show that inducible downmodulation of PCGF1 strongly impaired the growth of primary MLL-AF9 cells. Next, a Tet-regulated miR-E PCGF1 human xenograft MLL-AF9 leukemia mouse model was established, which revealed that early knockdown of PCGF1 at the onset of leukemia development significantly reduced peripheral blood chimerism levels and improved overall survival. In contrast, knockdown of PCGF1 when leukemia was already firmly established in the bone marrow proved insufficient to enhance overall survival. Despite these findings, FACS analysis of MLL-AF9/miR-E PCGF1/CD45+/GFP+ populations suggested that particularly cells with inefficient PCGF1 knockdown contributed to leukemogenesis. In conclusion, by building in vivo xenograft leukemia inducible RNAi models, we show that timing of gene knockdown critically impacts on the efficacy of leukemia treatment and that clonal drift still plays a major role in the escape of LSCs.
A randomized nonmonotone adaptive trust region method based on the simulated annealing strategy for unconstrained optimization
Authors: Babaie-Kafaki Saman , Rezaee Saeed 
Published: July 2019

PurposeThe purpose of this paper is to employ stochastic techniques to increase efficiency of the classical algorithms for solving nonlinear optimization problems.Design/methodology/approachThe well-known simulated annealing strategy is employed to search successive neighborhoods of the classical trust region (TR) algorithm.FindingsAn adaptive formula for computing the TR radius is suggested based on an eigenvalue analysis conducted on the memoryless Broyden-Fletcher-Goldfarb-Shanno updating formula. Also, a (heuristic) randomized adaptive TR algorithm is developed for solving unconstrained optimization problems. Results of computational experiments on a set of CUTEr test problems show that the proposed randomization scheme can enhance efficiency of the TR methods.Practical implicationsThe algorithm can be effectively used for solving the optimization problems which appear in engineering, economics, management, industry and other areas.Originality/valueThe proposed randomization scheme improves computational costs of the classical TR algorithm. Especially, the suggested algorithm avoids resolving the TR subproblems for many times.
What do mission statements reveal about the values of top universities in the world?
Authors: Breznik Kristijan , Law Kris M.Y. 
Published: July 2019

PurposeMission statements are perceived as effective strategic tools and foundations of an organization's objectives, driving the development of strategic plans to meet the established goals. A vast number of studies have investigated mission statements in industry; however, little is known on how mission statements affect institutes in the higher education sector. Every university is intrinsically different, with its own mission and goals. This study aims to explore how mission statements make the difference among universities, by understanding the values delivered by the mission statements.Design/methodology/approachThis study attempts to understand the core values of universities through a content analysis of the mission statements of the top 250 universities worldwide.FindingsThe results show four core dimensions in university mission statements, namely, “Education Philosophies”, “Strategic Orientation”, “Social Responsibility” and “Values to Stakeholders”, while the most common core values are “Research”, “Educate” and “Student”.Research limitations/implicationsMission statements offer insightful venues for the analysis of the strategic directions of universities. The results show that top universities present their core values through their mission statements. Some mission statements are more service-oriented (towards students) and some are more social-oriented (towards society and stakeholders). These mission statements are communicated to various stakeholders. “Education philosophies” are communicated to educators and students, “Social responsibility” is to the society, “Values to the stakeholders” are towards the different parties involved in the education system and the last one “Strategic orientation” is for the strategy setting.Originality/valueThis paper proposes a new methodology of mission statement studies, covering not only studies on mission statements but also the internationalization strategies of universities, which attract attention from academics and education researchers.
Better healthcare setting for better healthcare service quality
Authors: Kondasani Rama Koteswara Rao , Panda Rajeev Kumar , Basu R. 
Published: July 2019

Purpose
The purpose of this paper is to assess and compare different private healthcare settings based on perceived service quality in Indian context using analytical hierarchy process (AHP). The Indian private healthcare sector has been controlled by three categories of healthcare settings, namely, nursing clinics (NCs), non-corporate hospitals (NCHs) and corporate hospitals (CHs).

Design/methodology/approach
AHP was used to rank order of healthcare setting regarding the service quality dimensions and relative standings of every service provider with respect to its competitors. The authors collected random data from nine private hospitals situated in eastern and southern India. The authors proposed a feasible appraising approach based on AHP to decide the sustainability priority of dimensions like reliability, physical environment, empathy, efficiency, timeliness, transparency, affordability, communication, appropriateness and consistency in the Indian private healthcare industry.

Findings
The result demonstrates that Indian NCHs are performing better on customers’ perceived service quality, followed by CHs and NCs. The comparative performance of healthcare settings is an attempt to establish a performance ranking.

Originality/value
This research assesses dimensions of the service quality that need urgent attention. The results may provide insight to healthcare managers as to how they can improve their service quality in order to match customer expectation and to improve business performance.
Wear behaviors of WC and TiC on co matrix composites under three-body impact abrasive wear condition
Authors: Dong Lei , Zhang Xiaoyu , Liu Kun , Liu Xiaojun , Shi Ruiming , Wang Junyuan , Liu Feng 
Published: July 2019

Purpose
The purpose of this paper is to investigate the tribological properties of the WC/TiC-Co substrate under different loading conditions under three impact abrasive wear conditions.

Design/methodology/approach
The three body collisional wear behavior of Co alloy with WC and TiC at three impact energy was studied from 1 to 3 J. Meanwhile, the microstructure, hardness, phase transformation and wear behavior of these specimens were investigated by scanning electron microscopy, Rockwell hardness (HRV), EDS and impact wear tester. The resulting wear rate was quantified by electronic balance measurements under different pressures.

Findings
The specific wear rate increases with the increase of the nonlinearity of the impact energy and the increase in the content of WC or TiC. The effect of TiC on wear rate is greater than that of WC, but the hardness is smaller. The wear characteristics of the samples are mainly characterized by three kinds of behavior, such as cutting wear, abrasive wear and strain fatigue wear. The WC-Co with fewer TiC samples suffered heavier abrasive wear than the more TiC samples under both low and high impact energy and underwent fewer strain fatigue wears under high impact energy.

Originality/value
The experimental results show that the wear resistance of the Co alloy is improved effectively and the excellent impact wear performance is achieved. The results can be used in cutting tools such as coal mine cutting machines or other fields.
Analysis on suitability of HVOF sprayed Ni-20Al, Ni-20Cr and Al-20Ti coatings in coal-ash slurry conditions using artificial neural network model
Authors: Singh Jashanpreet 
Published: July 2019

Purpose
The purpose of this study is to analyze the slurry erosion failure of Ni-20Cr (Ni-Cr2O3), Ni-20Al (Ni-Al2O3) and Al-20Ti (Al2O3-TiO2) coatings deposited on SS316L by the high-velocity oxy-fuel process.

Design/methodology/approach
Slurry erosion experiments were conducted using a pot type erosion tester at different velocities 1.81, 2.71, 3.61 and 4.59 m/s for the time duration of 90-180 minutes. Fly ash and bottom ash were used as erodent media; the concentration of mass flux was taken as 30-60 wt. per cent. Artificial neural network (ANN) method was used to simulate the slurry erosion for thermally sprayed coatings.

Findings
Slurry erosion of coatings increases non-linearly with an increase in experimental durations, mass flux and velocity. Slurry erosion of Ni-20Cr and Ni-20Al layers was found to be maximum at 60° impingement angle, whereas 30° for SS316L and 45° for Al-20Ti coating. Slurry erosion performance of SS316L was improved by 2.56-3.19 times by depositing Ni-20Cr and Ni-20Al layers, whereas it improved 1.15-1.75 times by Al-20Ti coating. The slurry erosion SS316L was found almost 1.35 ± 1.28 times greater than that of the Ni-20Al coating, whereas it was to be 1.12 ± 1.36 times greater than Al-20Ti. Ni-20Al-coated SS316L showed a lower value of slurry erosion than Al-20Ti-coated SS316L.

Practical implications
Stainless Steel SS316L is widely used in hydraulic machinery (such as turbines, pumps, valves, fittings, etc.) of hydraulic and thermal power plants, chemical industry and marine industry. Therefore, the deposition of ductile and brittle coatings is a better option for their durable performance.

Originality/value
Erosion wear of Ni-20Cr, Ni-20Al and Al-20Ti coatings was successfully simulated by using an artificial neural network model by supplying experimental data as a target.
Finance-growth nexus: Islamic finance development in Indonesia
Authors: Al Fathan Ryanda , Arundina Tika 
Published: July 2019

Purpose
There are many studies related to finance-growth nexus, but existing empirical evidences still have not provided conclusive result of the nature and direction of this relationship. Moreover, there are only few studies about finance-growth nexus seen from Islamic finance perspective, especially in Indonesia. Therefore, this study aims to examine the nature of causal relationship between Islamic finance development and economic growth in Indonesia seen from the development of Islamic banking, sukuk market and Islamic stock market.

Design/methodology/approach
By using quarterly data from 2002Q3 to 2017Q4, this study uses vector autoregressive (VAR) model, then uses granger causality and impulse response function to analyze the causal relationship between Islamic finance development and economic growth and also among three main sub-sectors of Islamic finance.

Findings
This study found that Islamic banking development and Islamic stock market development support neutrality hypotheses view, while sukuk market development supports supply-leading hypotheses view. Moreover, this study also found that there are unidirectional causalities from sukuk market development to Islamic banking development and from sukuk market development to Islamic stock market development.

Research limitations/implications
This study focuses only on the development of Islamic finance viewed from a macro perspective and only looks at how the three main sub-sectors in Islamic finance develop. In addition, the results of research related to finance-growth nexus are also sensitive to the object of research, the method and the proxies of variables used.

Originality/value
To the best of the authors’ knowledge, there is no study that examines the causal relationship between Islamic finance development and economic growth in Indonesia based on its three main sub-sectors simultaneously. So, this study gives empirical evidence to contribute on finance-growth nexus discussion based on three main sub-sectors of Islamic finance development in Indonesia.
Cybersecurity economics – balancing operational security spending
Authors: Ekelund Stale , Iskoujina Zilia 
Published: July 2019

Purpose
The purpose of this paper is to demonstrate how to find the optimal investment level in protecting an organisation’s assets.

Design/methodology/approach
This study integrates a case study of an international financial organisation with various methods and theories in security economics and mathematics, such as value-at-risk (VaR), Monte Carlo simulation, exponential and Poisson probability distributions. Thereby it combines theory and empirical findings to establish a new approach to determining optimal security investment levels.

Findings
The results indicate that optimal security investment levels can be found through computer simulation with historical incident data to find VaR. By combining various scenarios, the convex graph of the risk cost function has been plotted, where the minimum of the graph represents the optimal invest level for an asset.

Research limitations/implications
The limitations of the research include a modest number of loss observations from one case study, and the use of normal probability distribution. The approach has limitations where there are no historical data available or the data has zero losses. These areas should undergo further research including larger data set of losses and exploring other probability distributions.

Practical implications
The results can be used by leading business practitioners to assist them with decision making on investment to the increased protection of an asset.

Originality/value
The originality of this research is in its new way of combining theories with historical data to create methods to measure theoretical and empirical strength of a control (or set of controls) and translating it to loss probabilities and loss sizes.
How do enterprise social media affordances affect social network ties and job performance?
Authors: Chen Xiayu , Wei Shaobo , Davison Robert M. , Rice Ronald E. 
Published: July 2019

PurposeThe purpose of this paper is to investigate how four enterprise social media (ESM) affordances (visibility, association, editability and persistence) affect social network ties (instrumental and expressive), which, in turn, influence the in-role and innovative job performance of employees.Design/methodology/approachA survey of 251 ESM users in the workplace in China was conducted.FindingsAll four affordances are positively associated with instrumental ties, yet only the association and editability affordances are positively related to expressive ties. Although instrumental and expressive ties are positively related to in-role and innovative job performance, instrumental ties exert stronger effects on in-role job performance, whereas expressive ties show stronger effects on innovative job performance.Research limitations/implicationsFirst, additional relevant affordances should be included in an expanded model. Second, future research could examine how patterns of affordances use (unrelated, or hierarchically or sequentially related) affect organizational network ties. Third, there are likely (many) other exogenous factors affecting the model’s relationships. Fourth, the data collected are self-reported.Practical implicationsThis study advances the theoretical understanding of the role of ESM affordances in the workplace, especially through their influences on network ties. The findings can guide organizations on how to emphasize ESM affordances to foster instrumental and expressive ties to improve the job performance of employees.Originality/valueFirst, it provides novel views on affordance theory in ESM contexts by empirically testing four central affordances, thereby further providing preliminary evidence for prior theoretical propositions by confirming that social media affordances might be associated with or influence relational ties. Second, the study integrates an affordance lens and a social network perspective to investigate employees’ perceived performance behavior. Including social network ties can offer a more detailed understanding of the underlying processes of how ESM affordances can and do affect job performance. Third, it supports the validity of distinguishing instrumental and expressive ties in ESM contexts, thus offering a possible explanation for the inconsistencies in prior research on the impact of social networks on employee outcomes. Finally, it also shows how two kinds of organizational performance (in-role and innovative) are somewhat differentially influenced by affordances and network ties.
Drivers of purchase behavior and post-purchase evaluation in the Singles’ Day promotion
Authors: Wang Lingli , Yan Qiang , Chen Wenjing 
Published: July 2019

Purpose
The purpose of this study is to examine the strategies used by consumers to control themselves in the Singles’ Day promotion. It also examines how promotion and social influence affect consumers’ purchase behavior and post-purchase evaluation.

Design/methodology/approach
A mixed methods approach including a quantitative study (N = 480) and a follow-up qualitative study (N = 35) was conducted to verify the hypotheses and provide deeper insights.

Findings
This study demonstrates that consumers allocate in-store slack in shopping budgets to restrict unplanned purchases and in-store slack is positively related to post-purchase evaluation. Social influence, which helps consumers rationalize purchase decisions, has positive effects on planned purchases and post-purchase evaluation. Both promotion strength and promotion range moderate the relationship between in-store slack and unplanned purchases.

Originality/value
Most studies investigate how promotion designs affect consumers’ in-store decision-making. This study focuses on the Singles’ Day promotion in China and examines the tactics consumers use to control purchase behavior in the promotion.
Does service quality increase the level of banks performance
Authors: Ali Syed Faraz , Naeem Muhammad 
Published: July 2019

PurposeThe purpose of this paper is to unfold the relationship between service quality and level of performance of conventional and Islamic banks. Also, it intends to uncover what are the features of service quality which can raise the level of performance either in conventional banks or Islamic banks. There is rare literature available that focused on comparative study between above stated banking systems based on emerging parameters of SERVQUAL model.Design/methodology/approachTo meet the objectives of this investigation, research data has been from 450 customers who have had accounts and dealings with conventional and Islamic banks in the previous five years. The customers are selected based on cluster sampling from regional offices of conventional and Islamic banks.FindingsThe collected data have been analyzed by using confirmatory factor analysis (CFA) technique followed by common method variance (CMV), multiple regression test and independent samplet-test used to examine the parameters of service quality in the context of banks performance. The purpose of CFA is to find the model validity, while multiple regression and t-test is performed in order to examine the influence of service quality parameters on banks performance.Originality/valueThe study used compliance as a one of the emerging and unique dimension of service quality. This dimension is rarely investigated in the context of measuring the level of bank performance of conventional and Islamic banking systems. Findings reveal responsiveness and assurance is the strongest predictor of conventional banking performance. Compliance and reliability has significant and positive impact on the level of performance of Islamic banks. Moreover, the study has practical implications for the top management and stakeholders of conventional and Islamic banks to increase the level of performance by using SERVQUAL model.
Shear tab connection with composite beam subjected to transient-state fire temperatures
Authors: Hajjar Mohammad , Hantouche Elie , El Ghor Ahmad 
Published: July 2019

Purpose
This study aims to develop a rational model to predict the thermal axial forces developed in shear tab connections with composite beams when subjected to transient-state fire temperatures.

Design/methodology/approach
Finite element (FE) models are first developed in ABAQUS and validated against experimental data available in the literature. Second, a parametric study is conducted to identify the major parameters that affect the behavior of shear tab connections with composite beams in the fire. This includes beam length, shear tab thickness, shear tab location, concrete slab thickness, setback distance and partial composite action. A design-oriented model is developed to predict the thermal induced axial forces during the heating and cooling phases of a fire event. The model consists of multi-linear springs that can predict the stiffness and strength of each component of the connection with the composite beam.

Findings
The FE results show that significant thermal axial forces are generated in the composite beam in the fire. This is prominent when the beam bottom flange comes in contact with the column. Fracture at the toe of the welds governs the behavior during the cooling phase in most FE simulations. Also, the rational model is validated against the FE results and is capable of predicting the thermal axial forces developed in shear tab connections with composite beams under different geometrical properties.

Originality/value
The proposed model can predict the thermal axial force demand and can be used in performance-based approaches in future structural fire engineering applications.