bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differences in intention to receive clinician-collected and self-collected samples for HPV DNA testing and its determinants between heterosexual males and females in Hong Kong, China: findings of a territory-wide household survey
Authors: Wang Zixin , Chen Siyu , Wong Ngai Sze , Cheung Annie Wai-Ling , Tam Zoe Pui-Yee , Chung Sze Long , Chan Denise Pui-Chung , Mo Phoenix K. H. , Wong Eliza Lai-Yi 
Published: December 2024

Abstract
Background
Human papillomavirus (HPV) infection is prevalent among people who are sexually active. This study aimed to compare the levels of behavioral intention to receive free clinician-collected and self-collected samples for HPV DNA testing and its determinants between heterosexual males and females in Hong Kong, China.

Methods
This is a secondary analysis of a territory-wide survey conducted in Hong Kong between May 2021 and March 2022. Participants were sexually active adult Hong Kong residents who were able to communicate in English or Chinese. Invitation letters were mailed to residential addresses that were geographically randomly selected. All sexually active adult household members were invited to complete a self-administered online survey. Multivariate logistic regression analyses were fitted.

Results
Out of 45,394 invitations, 1265 surveys were collected, and 487 heterosexual males and 741 females were included in the analysis. More females than heterosexual males intended to take up free clinician-collected samples for HPV DNA testing (76.7% versus 62.2%, p < 0.001). Similar proportion of heterosexual males and females intended to receive free self-collected samples for HPV DNA testing (67.8% versus 72.6%, p = 0.20). Perceived existing treatment could control sexually transmitted infections (STI) (treatment control), more concerned about STI (concern), perceived more benefits, cue to action (suggested by significant others), and self-efficacy related to HPV testing were associated with higher intention to receive clinician-collected samples for HPV testing in both groups. Heterosexual males who perceived more severe symptoms if contracted STI (identity), longer duration of STI (timeline), more negative effects of STI on their lives (consequences), more understanding of STI (coherence), and stronger negative emotions if contracted STI (emotions) also had higher behavioral intention to take up clinician-collected samples for HPV testing. In addition, perceived more benefits, cue to action, and self-efficacy related to self-collected samples for HPV DNA testing were associated with behavioral intention to take up such testing in both groups.

Conclusions
HPV DNA testing was under-utilized in Hong Kong. Free self-collected samples for HPV testing were highly acceptable by both heterosexual males and females. Illness representation of STI and the Health Belief Model could explain intentions to take up HPV DNA testing.
Subcutaneous inoculation of Escherichia coli in broiler chickens causes cellulitis and elicits innate and specific immune responses
Authors: Jonare Liv , Wattrang Eva , Östlund Emma , Wall Helena , Jacobson Magdalena , Jansson Désirée S. 
Published: December 2024

Abstract
Background
Cellulitis caused by Escherichia coli is a common cause of condemnation of broiler chickens at slaughter worldwide and is associated with economic losses and a possible negative impact on animal welfare. The study objective was to monitor clinical signs and immune responses after subcutaneous E. coli inoculation (1.1–1.8 × 107 CFU), aiming to induce cellulitis. Three groups of broiler chickens (n = 15/group) were inoculated with well-characterized E. coli strains (group A: ECA18 O24:H4/ST117 and group B: ECB11 O153:H9/ST38) or with saline (control) at 22 days-of-age. Clinical signs of disease, body weight and immune parameters were monitored until euthanasia 12–14 days after inoculation followed by post-mortem examination.

Results
The daily weight gain of the inoculated chickens was significantly lower one day after inoculation compared to the controls. Seven (23%) of the inoculated chickens displayed clinical signs: ruffled feathers, mild weakness, open-beak breathing and/or reluctance to stand, of which two birds were euthanized and one bird died. Five chickens in group B were observed with bacteraemia, which lasted up to three days after inoculation for two chickens. A transient increase in chicken mannose receptor MRC1L-B expression on circulating monocytes was observed one day after inoculation in both E. coli inoculated groups, with a more pronounced increase in group B. On day 7 after inoculation, the in vitro adherence of heterophils, monocytes and thrombocytes to the inoculated strain was increased in group B. Antibody titers to the inoculation strains were increased in some chickens in both groups on days 7 and 14 after inoculation, with the highest titers in group B. Seven (47%) and 13 (87%) of the chickens in group A and B, respectively, were diagnosed with cellulitis at post-mortem examination. In most birds, lesions consisted of plaque-like material embedded in the subcutaneous tissue of the abdominal wall.

Conclusions
Inoculation of E. coli caused cellulitis and prompted a rapid activation/redistribution of circulating monocytes followed by antibody production. The responses were most pronounced in chickens inoculated with E. coli strain ECB11, presumably because of a higher virulence.
The Yin and Yang of hsa-miR-1244 expression levels during activation of the UPR control cell fate
Authors: Czechowicz Paulina , Gebert Magdalena , Bartoszewska Sylwia , Kalinowski Leszek , Collawn James F. , Bartoszewski Rafal 
Published: December 2024

AbstractRegulation of endoplasmic reticulum (ER) homeostasis plays a critical role in maintaining cell survival. When ER stress occurs, a network of three pathways called the unfolded protein response (UPR) is activated to reestablish homeostasis. While it is known that there is cross-talk between these pathways, how this complex network is regulated is not entirely clear. Using human cancer and non-cancer cell lines, two different genome-wide approaches, and two different ER stress models, we searched for miRNAs that were decreased during the UPR and surprisingly found only one, miR-1244, that was found under all these conditions. We also verified that ER-stress related downregulation of miR-1244 expression occurred with 5 different ER stressors and was confirmed in another human cell line (HeLa S3). These analyses demonstrated that the outcome of this reduction during ER stress supported both IRE1 signaling and elevated BIP expression. Further analysis using inhibitors specific for IRE1, ATF6, and PERK also revealed that this miRNA is impacted by all three pathways of the UPR. This is the first example of a complex mechanism by which this miRNA serves as a regulatory check point for all 3 pathways that is switched off during UPR activation. In summary, the results indicate that ER stress reduction of miR-1244 expression contributes to the pro-survival arm of UPR.
Bovine leukemia virus (BLV) and risk of breast cancer; a systematic review and meta-analysis
Authors: Saeedi-Moghaddam Fateme , Mohammaditabar Mahdi , Mozhgani Sayed-Hamidreza 
Published: December 2024

Abstract
Background
The role of viruses in the development of breast cancer has been a subject of debate and extensive research over the past few decades. Several studies have examined the association between Bovine leukemia virus (BLV) infection and the risk of developing breast cancer; however, their findings have yielded inconsistent results. To address this uncertainty, the purpose of the present study was to conduct a systematic review and meta-analysis to determine any potential association between BLV and breast cancer.

Methods
The literature search was performed by finding related articles from PubMed, Web of Science, Scopus, EMBASE, and ScienceDirect databases. Statistical analysis was conducted using the meta package in R Studio and Review Manager 5.1. The I2 test was used to assess between-study heterogeneity. The Mantel-Haenszel method calculated the pooled odds ratio and its 95% confidence interval. Studies were divided into subgroups for comparison.

Results
The literature search identified a total of 17 studies that were deemed suitable for inclusion in the systematic review. Out of these 17 studies, 12 were used in the subsequent meta-analysis. Combining the data from these eligible studies, we calculated the pooled multi-factor adjusted odds ratio (OR) and a 95% confidence interval (CI). Considering the heterogeneity observed across the studies, the result obtained using the fixed effects model was 2.12 (1.77, 2.54). However, upon removing the six studies that contributed significantly to the heterogeneity, the pooled OR with a 95% CI was recalculated to be 3.92 (2.98, 5.16).

Conclusion
The result of this study suggests that BLV infection is statistically associated with Breast cancer.
Outcomes and experiences of music workshops for adolescents with depression and anxiety: An exploratory noncontrolled trial in Bogotá
Authors: Gómez-Restrepo Carlos , Roldan María Camila , Ariza-Salazar Karen , Godoy-Casasbuenas Natalia , Arenas Catherine Surace , Heritage Paul , Uribe-Restrepo José Miguel , Fung Catherine , Priebe Stefan 
Published: December 2024

Abstract
Objective
Adolescents frequently experience mental distress. However, there is little research on community activities that help adolescents recover from depression and anxiety. This study investigated the outcomes and experiences of music workshops for helping adolescents overcome anxiety and/or depression.

Results
Thirty-four participants aged 15 and 16 years were recruited from a cohort study of adolescents with symptoms of anxiety and/or depression and invited to participate in a musical education and practice workshop consisting of five weekly sessions. At the beginning and end of the workshops, symptoms of anxiety and depression were self-rated on the GAD-7 and PHQ-8. Experiences were assessed on a brief questionnaire with open-ended questions. The average attendance of groups was 86%, but only 56% of participants attended all five sessions of the workshops. The symptom levels did not significantly change during the intervention. Experiences were largely positive. Participants appreciated the interactions in the group and the learning of new skills. Some criticized the workshops as too short and found the logistics of attending difficult.

Trial registration
Current Controlled Trials ISRCTN50583823. Date Applied 18/03/2022.
Opioid administration across racial and ethnic groups for patients undergoing liver resection: are there disparities?
Authors: Stannard Blaine , Ninh Allen , Mroz Victoria , Ouyang Yuxia , Egorova Natalia N. , DeMaria Samuel , Wang Ryan 
Published: December 2024

Abstract
Background
Racial and ethnic disparities in the treatment of perioperative pain have not been well-studied, despite being observed in a variety of other medical settings. The goal of this investigation was to evaluate the relationship between race and ethnicity and intra- and postoperative opioid administration for patients undergoing open liver resection surgery.

Methods
In this single-center retrospective cohort study, adult patients undergoing open liver resection from January 2012 to May 2019 were identified. Demographic, intraoperative, and postoperative data were extracted from the institutional perioperative data warehouse. The primary outcome was weight-based intraoperative morphine milligram equivalents (MME/kg). Secondary outcome variables included use of neuraxial analgesia and length of stay (LOS). Multivariable regression models were used, which controlled for pertinent factors such as age and duration of surgery.

Results
There were 1294 adult open liver resections included in this study: 532 (41%) patients self-reported as White, 401 (31%) as Asian, 159 (12%) as Black, 97 (7%) as Hispanic, and 105 (8%) as Other. The risk adjusted mean intraoperative MME/kg was not different among racial groups (White: 3.25 [95% CL 3.02–3.49] mg/kg vs. Asian: 3.38 [95% CL 3.10–3.69] mg/kg, p = 0.87; Black: 2.95 [95% CL 2.70–3.23] mg/kg, p = 0.19; Hispanic: 3.36 [95% CL 3.00–3.77] mg/kg, p = 0.97). In the multivariable models for secondary outcomes, length of stay was significantly higher for Black (estimate: 1.17, CL: 1.00 to 1.35, p = 0.047) and Hispanic (1.30, CL: 1.05 to 1.65, p = 0.018) patients relative to White patients. No racial/ethnic groups were significantly associated with higher or lower odds of receiving regional anesthesia.

Conclusions
For patients undergoing liver resection surgery, no racial and ethnic disparities were observed for weight-based intraoperative MME.
High school diploma is associated with longer postpartum leukocyte telomere length in a cohort of primarily Latina women
Authors: Sumesh Dhanya , Lin Jue , Wojcicki Janet M 
Published: December 2024

Abstract
Objective
This study investigates correlates of maternal leukocyte telomere length (LTL) in the immediate postpartum period using a cross-sectional study design from an existing prospective longitudinal birth cohort of primarily Latina women. The study focuses on the role of maternal health and dietary habits in pregnancy and maternal education level and LTL at delivery.

Study Design
Latina mothers were recruited during the immediate postpartum period prior to 24 h at two San Francisco hospitals and dried blood spots were collected for LTL analysis via quantitative polymerase chain reaction (qPCR). We used multivariable linear regression models to determine independent predictors of maternal LTL during the postpartum period.

Results
In multivariable regression models, increasing maternal age was associated with shorter LTL during the immediate postpartum period (Coeff − 0.015; p < 0.01) whereas having a high school diploma was associated with longer LTL versus not having graduated from high school (Coeff 0.12; p < 0.01).

Conclusion
Maternal education level as a potential marker of exposure to life stressors and socioeconomic status was associated with maternal LTL after adjusting for age and other potential confounders in women of reproductive age.
A transphyletic study of metazoan β-catenin protein complexes
Authors: Mbogo Ivan , Kawano Chihiro , Nakamura Ryotaro , Tsuchiya Yuko , Villar-Briones Alejandro , Hirao Yoshitoshi , Yasuoka Yuuri , Hayakawa Eisuke , Tomii Kentaro , Watanabe Hiroshi 
Published: December 2024

AbstractBeta-catenin is essential for diverse biological processes, such as body axis determination and cell differentiation, during metazoan embryonic development. Beta-catenin is thought to exert such functions through complexes formed with various proteins. Although β-catenin complex proteins have been identified in several bilaterians, little is known about the structural and functional properties of β-catenin complexes in early metazoan evolution. In the present study, we performed a comparative analysis of β-catenin sequences in nonbilaterian lineages that diverged early in metazoan evolution. We also carried out transphyletic function experiments with β-catenin from nonbilaterian metazoans using developing Xenopus embryos, including secondary axis induction in embryos and proteomic analysis of β-catenin protein complexes. Comparative functional analysis of nonbilaterian β-catenins demonstrated sequence characteristics important for β-catenin functions, and the deep origin and evolutionary conservation of the cadherin–catenin complex. Proteins that co-immunoprecipitated with β-catenin included several proteins conserved among metazoans. These data provide new insights into the conserved repertoire of β-catenin complexes.
Shifting waves of shipping: a review on global shipping projections and methodologies
Authors: Naghash Hesam , Schott Dingena , Pruyn Jeroen 
Published: December 2024

AbstractAs climate change continues to pose a significant threat to our planet, international maritime shipping plays a crucial role in mitigation efforts. Recognizing the urgency, the International Maritime Organization (IMO) has revised its targets, now aiming for full decarbonization by 2050. However, there is no established pathway to get to the target. To achieve this, there is a need for models depicting possible futures of the maritime sector, and finding feasible pathways. This research aims to find the most suitable way to develop models to find pathways toward decarbonization targets. This involves evaluating existing ranges and scenarios to understand current estimations and their underlying assumptions and assessing the most suitable modeling methods based on defined criteria. Considering the context, the most suitable models for this objective should perform on a global scale. They should include dynamics between shipping demand & supply as well as the derived fuel demand and supply and emissions; integrate the sector with other parts of the economy; incorporate various technologies into the framework; and span multiple scenarios. The study has two main parts. First, existing scenarios on the future of maritime shipping are analyzed to identify current estimations and assumptions impacting these estimations. Second, various modeling frameworks are assessed against the defined criteria to identify the most suitable modeling structure for achieving the decarbonization targets. Many projections do not meet the IMO’s updated targets, highlighting the need for a paradigm shift in setting targets and finding feasible pathways rather than focusing solely on individual measures. Integrated Assessment Models (IAMs) have been identified as suitable for such projections and policy analysis, although international shipping is often underrepresented in current models. Future research should combine the insights of sectoral models in integrated frameworks such as IAMs to develop integrated strategies to investigate pathways to achieve zero-emission targets. The ultimate goal is to understand how to effectively reduce the sector’s emissions and achieve more environmentally friendly international maritime shipping.
Validation of the German version of the state mindfulness scale for physical activity in a clinical sample
Authors: Wallner Jan , Kind Leona , Donath Carolin , Kornhuber Johannes , Luttenberger Katharina 
Published: December 2024

Abstract
Background
Mindfulness has been studied as a treatment option for a large range of psychological disorders and is associated with a multitude of positive psychological outcomes. There are now several scales for measuring mindfulness as both a trait and a state. As mindfulness potentially plays a critical role in maintaining physical activity habits, the State Mindfulness Scale for Physical Activity (SMS-PA) was developed to specifically measure mindfulness in a physical activity context. This study aimed to provide validity evidence for a German version of the SMS-PA (henceforth called SMS-PA-G) in a clinical sample.

Methods
We used baseline data from 129 participants in the randomised controlled trial ‘New Ways to Cope with Depression’. The sample, which was screened for clinical depression symptoms, completed the SMS-PA-G and several other psychometric scales for capturing state mindfulness, self-efficacy, sense of coherence, anxiety, depression, and physical activity. We conducted reliability and item analyses and ran a confirmatory factor analysis. Also, we assessed construct validity through correlations with the abovementioned scales and through differences in SMS-PA scores between physically active and nonactive participants.

Results
The mean SMS-PA-G score in our sample was 25.3 with a standard deviation of 8.5. The item and reliability analyses provided satisfactory Cronbach’s alpha and discriminatory power values. The confirmatory factor analysis showed that physical activity mindfulness can best be described via a bifactor model, with specific mind and body factors and a general mindfulness factor. We found the expected relationships with the attention subscale of state mindfulness, self-efficacy, and sense of coherence but did not find them with the awareness subscale of state mindfulness, depression, and anxiety. As hypothesised, physically active participants exhibited higher SMS-PA-G values than nonactive participants.

Conclusions
The SMS-PA-G is an internally consistent test instrument that captures respondents’ general physical activity mindfulness and their attention to mental and bodily events. Whereas validity evidence was generally supportive of the SMS-PA-G, its relationships with other constructs require further investigation.

Trial registration
ISRCTN, ISRCTN12347878. Registered 28 March 2022, https://www.isrctn.com/ISRCTN12347878.
Sinking bone flap with ipsilateral hemiparesis: the neurosurgical paradox—a case report and literature review
Authors: Goyal Pawan , Gaonkar Vishwa Bharathi , Gupta Aditya 
Published: December 2024

Abstract
Background
One of the less known complications following cranioplasty is the sinking bone flap syndrome which usually occurs in patients with an accompanying ventriculo-peritoneal shunt which leads to intracranial hypotension. This condition is rarely seen in patients without a draining VP shunt. In this article, we describe the case of a patient who developed sinking bone flap syndrome without a VP shunt.

Case presentation
A 44-year-old gentleman with chronic liver disease developed altered sensorium following trivial falls and was diagnosed with an acute right fronto-temporo-parietal SDH for which he underwent decompressive craniectomy. Autologous cranioplasty was done three months following the procedure and patient was discharged with no deficits. He developed ipsilateral hemiparesis three years after cranioplasty and on evaluation was found to have a sinking bone flap. Redo cranioplasty was done with patient specific implant following which he improved neurologically.

Conclusion
Sinking bone flap syndrome can occur in the absence of VP shunt and patients should be regularly followed after cranioplasty to avoid development of neurological deficits.
Investigating the relationship between manual dexterity and auditory spatial processing
Authors: Jargar Ritwik , Upadhya Sushmitha , Bhattacharyya Rohit , Nisha K. V. 
Published: December 2024

Abstract
Background
Manual dexterity, referring to fine motor skills and hand–eye coordination, has been suggested to influence various cognitive processes, including attention. Attention has been shown to be strongly related to auditory spatial processing, which primarily involves the perception and interpretation of sound locations in the environment. This study aimed to investigate the potential association between manual dexterity and auditory spatial processing abilities.

Method
A total of 30 participants, aged 19–25 years (mean age: 22.06 ± 1.85 years), were assessed for handedness using the Edinburgh Handedness Questionnaire (EHQ). They were divided into 2 groups: 15 right-handed (RH) and 15 left-handed (LH) individuals based on their EHQ scores. The virtual auditory space identification (VASI) test was administered for the assessment of auditory spatial skills. It consists of presenting a series of virtually presented sounds from 8 azimuths (0°, R45°, R90°, R135°, 180°, L90°, L135°, and L45°) randomly through TDH HDA 200 headphones. Correct responses and biased responses along with their corresponding reaction times were calculated.

Results
Results revealed that although the RH group displayed a slightly stronger leftward bias in both degrees of error and reaction compared to the LH group and vice-versa, these differences were not statistically significant.

Conclusion
The study concluded that there is little to no association between handedness and an individual’s auditory spatial ability. The absence of a significant difference in auditory spatial performance between RH and LH individuals may contribute to more inclusive and equitable approaches in fields where auditory spatial abilities are important.

Trial registration
SH/IRB/RP/08. Registered 12 January 2023.