bluuebunny/crossref_metadata_2025_split huggingface dataset:
Naturally acquired IgG responses to Plasmodium falciparum do not target the conserved termini of the malaria vaccine candidate Merozoite Surface Protein 2
Authors: Zerebinski Julia , Margerie Lucille , Han Nan Sophia , Moll Maximilian , Ritvos Matias , Jahnmatz Peter , Ahlborg Niklas , Ngasala Billy , Rooth Ingegerd , Sjöberg Ronald , Sundling Christopher , Yman Victor , Färnert Anna , Plaza David Fernando 
Published: December 2024

IntroductionMalaria remains a significant burden, and a fully protective vaccine against Plasmodium falciparum is critical for reducing morbidity and mortality. Antibody responses against the blood-stage antigen Merozoite Surface Protein 2 (MSP2) are associated with protection from P. falciparum malaria, but its extensive polymorphism is a barrier to its development as a vaccine candidate. New tools, such as long-read sequencing and accurate protein structure modelling allow us to study the genetic diversity and immune responses towards antigens from clinical isolates with unprecedented detail. This study sought to better understand naturally acquired MSP2-specific antibody responses.MethodsIgG responses against recombinantly expressed full-length, central polymorphic regions, and peptides derived from the conserved termini of MSP2 variants sequenced from patient isolates, were tested in plasma from travelers with recent, acute malaria and from individuals living in an endemic area of Tanzania.ResultsIgG responses towards full MSP2 and truncated MSP2 antigens were variant specific. IgG antibodies in the plasma of first-time infected or previously exposed travelers did not recognize the conserved termini of expressed MSP2 variants by ELISA, but they bound 13-amino acid long linear epitopes from the termini in a custom-made peptide array. Alphafold3 modelling suggests extensive structural heterogeneity in the conserved termini upon antigen oligomerization. IgG from individuals living in an endemic region, many who were asymptomatically infected, did not recognize the conserved termini by ELISA.DiscussionOur results suggest that responses to the variable regions are critical for the development of naturally acquired immunity towards MSP2.
Vitronectin promotes proliferation and metastasis of cervical cancer cells via the epithelial-mesenchymal transition
Authors: Lin Yao , Bian Lihong , Zhu Guangwei , Zhang Bin 
Published: December 2024

BackgroundVitronectin (VTN) is a multifunctional glycoprotein in blood and the extracellular, which could be an effective biomarker for many cancers. However, its role in cervical cancer is under investigated. In this study, we aimed to determine the molecular function of VTN and its potential mechanism in cervical cancer (CC).Materials and methodsUp- and down-regulated VTN expression was determined in Hela and C33A cells. Reverse transcription, qRT-PCR, and Western blotting test were performed to identify VTN mRNA and protein levels, separately. CCK-8 assay and colony formation assay were carried out to evaluate proliferation abilities of CC cells. A scratch test and a transwell chamber assay were performed to determine cell migration and invasion ability. Expression levels of epithelial-mesenchymal transition (EMT)-related proteins were measured by Western blotting.ResultsCell models with up- and down-regulated VTN expression in Hela and C33A cells were successfully established, as confirmed by Western blotting and qPCR. CCK-8 and colony formation assays demonstrated that VTN overexpression significantly enhanced the proliferation of both Hela and C33A cells. Wound healing and Transwell migration assays further indicated that VTN overexpression markedly promoted the migratory and invasive capabilities of these cells. Moreover, Western blotting analysis revealed that VTN overexpression led to a decrease in ZO-1 and E-cadherin protein levels and an increase in β-catenin and N-cadherin levels, whereas VTN knockdown yielded the opposite effect. These findings suggest that VTN promotes cervical cancer cell malignancy through epithelial-mesenchymal transition (EMT).ConclusionVTN plays a tumor-promoting role in CC by promoting the EMT of cervical cancer cells.
Research progress of breast cancer surgery during 2010–2024: a bibliometric analysis
Authors: Kang Jiawei , Jiang Nan , Shataer Munire , Tuersong Tayier 
Published: December 2024

PurposeThis study seeks to systematically analyze the research literature pertaining to breast cancer surgery from 2010 to 2024, as indexed in the PubMed database, employing bibliometric methodologies.MethodsEmploying the “bibliometrix” package in the R programming language, alongside VOSviewer and CiteSpace software, this research conducted a comprehensive visual analysis of 1,195 publications. The analysis encompassed publication trends, collaborative networks, journal evaluation, author and institutional assessments, country-specific analyses, keyword exploration, and the identification of research hotspots.ResultsThe study observed a rising trend in the number of publications related to breast cancer surgery. However, there was a concomitant decline in citation rates, potentially indicating either a saturation of the research field or a diminution in research quality. The United States, China, and Japan are the leading contributors to research output, with the United States showing the most extensive international collaboration. The University of California, University of Toronto, and University of Texas MD Anderson Cancer Center were the top institutions for the number of published papers. Through a comprehensive analysis of keywords, we have identified “breast cancer” “pain” “anxiety” “lymphedema” “mastectomy” and “surgery” as central research themes within this domain, the corresponding clusters were subjected to analysis.ConclusionThis study provides a comprehensive review of breast cancer surgery research, emphasizing major research areas and proposing future research directions. This study provides a significant resource for researchers and clinicians in the field.
Effectiveness of a brief multicomponent intervention to improve physical activity level and functional capacity in fibromyalgia and chronic fatigue syndrome (Synchronize+)
Authors: Martín-Borràs Carme , González Serra Gemma , Carrasco-Querol Noèlia , Sansano-Nadal Oriol , Bueno Hernández Nerea , Bestraten del Pino Pau , Pastor Cazalla Marta , Caballol Angelats Rosa , Montesó-Curto Pilar , Castro Blanco Elisabet , Pozo Ariza Macarena , Fernández-Sáez José , Dalmau Llorca M. Rosa , Gonçalves Alessandra Queiroga , Aguilar Martín Carina 
Published: December 2024

IntroductionFibromyalgia (FM) and chronic fatigue syndrome (CFS) are complex central sensitization syndromes that represent an important public health problem. Low cardiorespiratory fitness and muscle function with habitual intolerance to efforts are common characteristics of FM and CFS. This study aimed to examine the effect of a brief multicomponent intervention based on physical activity (PA), nutrition, and chronobiology on movement behaviors (PA, sedentary and sleep time), muscle strength, and cardiorespiratory capacity.Methodsrandomized controlled trial was conducted in primary healthcare in Catalonia. A total of 143 individuals with FM or FM and CFS concomitantly (age 50.8, SD 8.1; 94.4% women) were randomly allocated to the intervention (IG, n = 69) or control (CG, n = 74) groups. The IG participated in a brief multicomponent (PA, nutrition, and chronobiology) group-based intervention (4 sessions, 3 h/session) while the CG received usual primary care practice. Primary outcome measure was PA measured by the REGICOR-Short Physical Activity Questionnaire. Secondary outcomes were sedentary (International Physical Activity Questionnaire) and sleep time (Pittsburgh Sleep Quality Index), upper- and lower-body muscle strength (handgrip and sit-to-stand test, respectively), and aerobic capacity (6-min walk test). Data were collected at baseline and 3 months post-intervention.ResultsThe IG showed positive differences at 3-month follow-up, with highly appreciably PA levels, less sedentary time, and significantly improved sleep time. Significant between-group differences were also observed at 3 months, with better health values in the IG: PA and sleep time (370.3 ± 307.0 vs. 195.9 ± 289.1 min/week and 6.1 ± 1.6 vs. 5.5 ± 1.8 h/night, respectively) and less sedentary time (266.2 ± 153.3 vs. 209.4 ± 199.9 min/day). The IG also showed higher upper limb strength and significant lower-body strength both between and within groups, as well as significantly improved cardiorespiratory capacity.ConclusionThe Synchronize + multicomponent program implemented at primary healthcare has shown short-term effectiveness in improving 24-h movement behaviors and health outcomes in individuals with FM, with or without CFS. This intervention may be a first step in educating and motivating people with FM and CFS to adopt an active lifestyle, leading to improved health. Long-term follow-up will determine whether the changes are maintained over time and their impact on quality of life and healthcare costs.
Update on NKCC2 regulation in the thick ascending limb (TAL) by membrane trafficking, phosphorylation, and protein-protein interactions
Authors: Maskey Dipak , Granados Pineda Jessica , Ortiz Pablo A. 
Published: December 2024

Purpose of reviewThe thick ascending limb (TAL) of loop of Henle is essential for NaCl, calcium and magnesium homeostasis, pH balance and for urine concentration. NKCC2 is the main transporter for NaCl reabsorption in the TAL and its regulation is very complex. There have been recent advancements toward understanding how NKCC2 is regulated by protein trafficking, protein-protein interaction, and phosphorylation/dephosphorylation. Here, we update the latest molecular mechanisms and players that control NKCC2 function, which gives an increasingly complex picture of NKKC2 regulation in the apical membrane of the TAL.Recent FindingsProtein-protein interactions are required as a regulatory mechanism in many cellular processes. A handful of proteins have been recently identified as an interacting partner of NKCC2, which play major roles in regulating NKCC2 trafficking and activity. New players in NKCC2 internalization and trafficking have been identified. NKCC2 activity is also regulated by kinases and phosphatases, and there have been developments in that area as well.SummaryHere we review the current understanding of apical trafficking of NKCC2 in the thick ascending limb (TAL) which is tightly controlled by protein-protein interactions, protein turnover and by phosphorylation and dephosphorylation. We discuss new proteins and processes that regulate NKCC2 that have physiological and pathological significance.
Spontaneous theory of mind in autism: are anticipatory gaze and reaction time biases consistent?
Authors: Onda Keigo , Ichwansyah Rizal , Kawasaki Keisuke , Egawa Jun , Someya Toshiyuki , Hasegawa Isao 
Published: December 2024

BackgroundIndividuals with autism spectrum disorder (ASD) exhibit persistent deficits in social interaction and communication in adulthood. Pioneering studies have suggested that these difficulties arise from a lack of immediate, spontaneous mentalizing (i.e., theory of mind: ToM), specifically, an ability to attribute false beliefs to others, which should be usually acquired during neurotypical development. However, this view has been challenged by recent reports of nonreplications of spontaneous mentalizing, even in neurotypical adults.ObjectivesWe aimed to evaluate (1) whether measurements of spontaneous ToM in two representative paradigms, gaze bias in the anticipatory looking (AL) test and reaction time bias in the object detection (OD) test, are correlated in neurotypical adults and (2) whether these two measurements are altered in individuals with ASD.MethodsWe developed a novel hybridized spontaneous false belief test combining the AL and OD paradigms to enable within-subject comparison of different spontaneous ToM measurements.ResultsThe results obtained with our hybridized test replicated the earlier positive evidence for spontaneous ToM in both AL and OD paradigms. Our results also revealed a correlation between the participants’ spontaneous gaze bias in the AL paradigm and reaction time bias in the OD paradigm, indicating that the participants who had spontaneously anticipated other’s false belief driven actions more quickly detected the object. We further found that spontaneous gaze and reaction time biases were altered in individuals with ASD. Finally, we ascertained those inclusions of these biases as diagnostic variables in a regression model improved the accuracy of diagnosing ASD. ASD diagnosis was best predicted by the model when variables obtained from both AL and OD methods were included in the model.Conclusions/implicationsOur hybridized paradigm not only replicated spontaneous gaze bias in early AL studies and reaction time bias in the OD paradigms, but indicated significant correlation between them, suggesting that different implicit tasks tap the same spontaneous ToM in neurotypical adults. Group differences of these indices between ASD and neurotypical adult groups indicated that our task could help diagnose ASD, which is essential for evaluating the social difficulties that individuals with ASD face in adulthood.
Association between phenotypic age and mortality risk in individuals with obesity: a retrospective cohort study
Authors: Huang Yingxuan , Zhou Apei , Huang Yisen , Wang Yubin , Liu Xiaobo , Liu Xiaoqiang 
Published: December 2024

ObjectiveThis study investigates the association between phenotypic age acceleration (PAA) and all-cause and cause-specific mortality in obese individuals.MethodsData were drawn from the National Health and Nutrition Examination Survey (NHANES) between 1999 and 2018, including 9,925 obese adults (BMI ≥ 30 kg/m2). PAA, defined as phenotypic age exceeding chronological age, was assessed using clinical biomarkers. Kaplan-Meier survival analysis and Cox proportional hazards models were used to assess the relationship between PAA and all-cause, cardiovascular, and cancer mortality, adjusting for covariates such as age, gender, race, lifestyle, and health status. Subgroup and sensitivity analyses were performed to ensure the robustness of the findings.ResultsDuring a median follow-up of 10.6 years, 1,537 deaths were recorded, including 419 from cardiovascular disease and 357 from cancer. PAA was significantly associated with all-cause mortality (HR = 1.84, 95% CI: 1.64–2.06), cardiovascular mortality (HR = 1.86, 95% CI: 1.50–2.31), and cancer mortality (HR = 1.47, 95% CI: 1.17–1.85). These associations remained significant after adjusting for multiple variables, and sensitivity analyses confirmed the robustness of the results.ConclusionPAA is an independent predictor of all-cause, cardiovascular, and cancer mortality in obese individuals. This study highlights the importance of PAA in mortality risk assessment and health management in the obese population.
Supernatants from Newly Isolated Lacticaseibacillus paracasei P4 Ameliorate Adipocyte Metabolism in Differentiated 3T3-L1 Cells
Authors: Grigorova Natalia , Ivanova Zhenya , Petrova Valeria , Vachkova Ekaterina , Beev Georgi 
Published: December 2024

Background: Lacticaseibacillus paracasei (L. paracasei) strains and their postbiotics show potential for managing metabolic disorders such as diabetes and obesity. Two newly isolated L. paracasei strains, M2.1 and P4, were yielded from Formica rufa anthills in Sinite Kamani National Park, Bulgaria. Their metabolic effects on mature 3T3-L1 adipocytes were investigated. Methods: Mature 3T3-L1 adipocytes were treated for 24 h with 10% (v/v) cell-free supernatants (CFSs) of M2.1 or P4. Two experimental (M2.1, P4) and two control groups (mature, untreated adipocytes and mature adipocytes, treated with 10% (v/v) MRS broth) were analyzed for intracellular lipid accumulation, glucose uptake, and the mRNA expression of lipid metabolism and beta-oxidation-related genes. Fold changes in gene expression were assessed using RT-qPCR. Results: Both M2.1 and P4 CFSs enhanced glucose uptake by over 30% compared to the control. P4 demonstrated a more favorable effect by significantly upregulating adipose triglyceride lipase–patatin-like phospholipase domain containing 2, adiponectin, and peroxisomal beta-oxidation enzymes—acyl-coenzyme A oxidase 1, palmitoyl. Intracellular lipid accumulation increased only with M2.1, while P4 supported improved lipid turnover without promoting excessive lipid storage or lipolysis. Conclusions: P4 CFS exhibits the potential to improve adipocyte metabolism by enhancing glucose uptake, promoting beta-oxidation, and increasing adiponectin expression, offering a promising strategy for managing metabolic dysfunctions.
Inclusion of Moringa oleifera Leaf Extracts and Varying Final Internal Temperatures to Influence the Antioxidant and Physicochemical Qualities of Low-Grade Beef Patties
Authors: Kartikasari Lilik Retna , Barido Farouq Heidar , Hertanto Bayu Setya , Nuhriawangsa Adi Magna Patriadi , Swastike Winny 
Published: December 2024

This study evaluated the efficacy of Moringa oleifera (M. oleifera) extract on the functional and physicochemical properties of low-grade beef patties after different final internal temperature cooking was carried out. Four ranges of concentration (1–4%) were compared to a negative control (without M. oleifera addition), wherein all treatments were cooked until reaching medium (71 °C) and well-done (80 °C) temperatures. The results showed that incorporating M. oleifera extract at 3% significantly reduced malondialdehyde (MDA) formation in medium-cooked patties compared to the control (p < 0.05), enhancing antioxidant capacity. A reduction in MDA in well-done patties was also shown up to this additional level. However, higher concentrations (4%) adversely affected water holding capacity, cooking yield, and proximate compositions. Further, the pH value also declined with increased M. oleifera extract. Therefore, this study revealed that cooking patties to well-done resulted in greater lipid oxidation and cooking loss. Further, this study recommends using 3% M. oleifera extract in medium-cooked beef patties to maximize the antioxidant benefits while maintaining quality.
Perception of antimicrobial stewardship interventions in Swiss primary care: a mixed-methods survey
Authors: Schaad Simeon , Dunaiceva Jelena , Peytremann Arnaud , Gendolla Sophie , Clack Lauren , Plüss-Suard Catherine , Niquille Anne , Nicolet Anna , Marti Joachim , Boillat-Blanco Noémie , Wolfensberger Aline , Mueller Yolanda 
Published: December 2024

BackgroundWith most of the antibiotic prescriptions occurring in primary care, antimicrobial stewardship (AMS) interventions must be known, welcomed, and used by primary care physicians (PCPs).AimThe main objective of this study was to evaluate the present awareness about, use of, and perceived acceptability, appropriateness, and feasibility of a broad range of interventions.Design & settingA cross-sectional survey was distributed to Swiss PCPs from December 2023 to February 2024.MethodThe survey focused on eight AMS interventions: shared decision-making tools, factsheets for physicians, Swiss Federal Office of Public Health (FOPH) information material, national antibiotic guidelines website, audit and feedback, communication skills training, as well as the use of point-of-care C-reactive protein (POC-CRP) and procalcitonin (POC-PCT) to guide prescription. PCPs’ perceived acceptability, appropriateness, and feasibility were assessed using five-point Likert scales. General expectations regarding AMS were evaluated via qualitative analysis of free-text answers.ResultsOut of 7456 potentially eligible primary care physicians, 355 PCPs answered at least one question (response rate 4.7%). PCPs were most aware of biomarkers to guide antibiotic prescription in RTIs, such as POC-PCT (67.6%) and POC-CRP (61.1%), the FOPH awareness campaign (57.3%) and the national guidelines website (52.7%). All interventions were rated as acceptable, appropriate, and feasible, with respective mean scores out of five of 3.89, 3.91, and 3.81.ConclusionDespite the high perceived acceptability, appropriateness, and feasibility of AMS interventions available for RTIs, their real-life impact may be hindered by insufficient awareness. Additional promotion of those tools could increase their uptake by physicians.
PENERAPAN INTERVENSI MIRROR THERAPY PADA ASUHAN KEPERAWATAN KELUARGA DENGAN DIAGNOSA MEDIS STROKE DI DESA LEBENGJUMUK KAB. GROBOGAN
Authors: Sari Hany Novita , Riniasih Wahyu 
Published: December 2024

Latarbelakang: Stroke merupakan istilah yang digunakan untuk menggambarkan perubahan neurologis yang disebabkan oleh adanya gangguan suplai darah kebagian dari otak (Keperawatan et al., 2018). Stroke didefinisikan oleh world health organization (WHO) sebagai perkembangan yang cepat dari tanda klinis dan gejala gangguan neulrologi fokal atau global yang terjadi lebih dari 24 jam. Stroke dapat menyebabkan kematian tanpa ditemukan penyebab lain, selain penyebab vaskuller (Eka Sukmawati & M Dirdjo, 2021). Penyakit stroke sangat berdampak pada fungsi ekstremitas baik bawah maupun atas. Dimana fungsi ekstremitas tersebut akan mengalami penurunan sehingga penderita stroke tidak dapat mengontrol ekstremitasnya dan juga kemampuan bergeraknya. Ekstremitas atas merupakan salah satu ektrelmitas dengan berbagai fungsi yang digunakan untuk menjalani hidup sehari-hari. Efek yang ditimbulkan dari penurunan fungsi ekstremitas tersebut terkhususnya ekstremitas atas adalah terganggunya aktivitas sehari-hari pasien seperti mandi, makan, bermain alat musik dan jenis-jenis aktivitas lainnya yang menggunakan bantulan ekstremitas atas (Setiyawan et al., 2019).Metode: Rancangan penelitian ini menggunakan rancangan penelitian studi kasus untuk mengeksplorasi masalah asuhan keperawatan keluarga pada Tn.R dengan fokus intervensi mirror therapy pada pasien stroke di Desa Lebengjumuk Kec. Grobogan Kab. Grobogan. Jenis penelitian ini menggunakan metode wawancara dan observasi. Subjek dalam penelitian ini adalah Tn R umur 65 tahun penderita stroke di Desa Lebengjumuk Kec. Grobogan Kab. Grobogan.Hasil: Peneliti mengidentifikasi bahwa setelah dilakukan intervensi mirror therapy pada pasien stroke selama 5 kali kunjungan berturut-turut, dapat memberikan pengaruh terhadap peningkatan kekuatan otot pasien dan kemampuan anggota gerak atas pasien pasca stroke, sebelum dilakukan therapy kekuatan otot 2, setelah dilakukan therapy kekuatan otot 3Kesimpulan: Terdapat pengaruh yang sangat signifikan dapat disimpulkan bahwa memberikan mirror therapy pada pasien stroke dapat meningkatkan kekuatan otot pasien dan kemampuan anggota gerak atas pasien pasca stroke