bluuebunny/crossref_metadata_2025_split huggingface dataset:
Performance of a Factory-Calibrated, Real-Time Continuous Glucose Monitoring System in Pediatric Participants With Type 1 Diabetes
Authors: Welsh John B. , Zhang Xiaohe , Puhr Sarah A. , Johnson Terri Kang , Walker Tomas C. , Balo Andrew K. , Price David 
Published: September 2018

Background: The perceived value and consistent use of continuous glucose monitoring (CGM) systems depends in part on their accuracy. We assessed the performance of a sixth-generation CGM system (Dexcom G6) in children and adolescents. Methods: Forty-nine participants (6-17 years of age, mean ± SD of 13.5 ± 3.3 years), all with type 1 diabetes, enrolled and data were available from 37. Each participant wore 1 sensor for up to 10 days and was asked to undergo an in-clinic visit lasting 6-12 hours for frequent blood glucose (BG) sample testing on one of the sensor wear days. Estimated glucose values (EGVs) from the G6 system were compared with venous BG values measured with a laboratory reference instrument (YSI). Results: The overall mean absolute relative difference (MARD) for 1387 EGV-YSI pairs was 7.7%, and the overall percentage of EGVs within 20% or 20 mg/dL of the YSI reference value (for YSI > or ⩽100 mg/dL, respectively, the “%20/20”) was 96.2%. The %20/20 was 92.1% on Day 1 and 91.0% on Day 10 of sensor wear. For EGVs <70 mg/dL, 92.6% of the YSI values were within 20 mg/dL and for EGVs >250 mg/dL, 100% of the YSI values were within 20%. Differences between EGVs and YSI values in over 99.9% of the pairs posed no or only slight clinical risk as evaluated by surveillance error grid analysis. Conclusions: The accuracy of the G6 CGM system in pediatrics may encourage consistent use of the system and contribute to improved glycemic outcomes in this population.
Blue light exposure decreases systolic blood pressure, arterial stiffness, and improves endothelial function in humans
Authors: Stern Manuel , Broja Melanie , Sansone Roberto , Gröne Michael , Skene Simon S , Liebmann Joerg , Suschek Christoph V , Born Matthias , Kelm Malte , Heiss Christian 
Published: September 2018

Aims Previous studies have shown that ultraviolet light can lead to the release of nitric oxide from the skin and decrease blood pressure. In contrast to visible light the local application of ultraviolet light bears a cancerogenic risk. Here, we investigated whether whole body exposure to visible blue light can also decrease blood pressure and increase endothelial function in healthy subjects. Methods In a randomised crossover study, 14 healthy male subjects were exposed on 2 days to monochromatic blue light or blue light with a filter foil (control light) over 30 minutes. We measured blood pressure (primary endpoint), heart rate, forearm vascular resistance, forearm blood flow, endothelial function (flow-mediated dilation), pulse wave velocity and plasma nitric oxide species, nitrite and nitroso compounds (secondary endpoints) during and up to 2 hours after exposure. Results Blue light exposure significantly decreased systolic blood pressure and increased heart rate as compared to control. In parallel, blue light significantly increased forearm blood flow, flow-mediated dilation, circulating nitric oxide species and nitroso compounds while it decreased forearm vascular resistance and pulse wave velocity. Conclusion Whole body irradiation with visible blue light at real world doses improves blood pressure, endothelial function and arterial stiffness by nitric oxide released from photolabile intracutanous nitric oxide metabolites into circulating blood.
Peer-reviewed Images: Image Consuming Selves as Visual CommoditiesNotes: All images used in this article are either part of the author's own archive or have been photographed by him, or he has been commissioned to do so. This article was initially prepared for presenting in a conference titled: ‘Creative Industries in Asia: Innovating within Constraints’, Bangkok University, 12 July 2016. The revised and advanced draft of this article was presented in ‘Visual South Asia Conference’, Department of Anthropology, University of Dhaka, 10-11 May 2017. This essay is a part of the larger book project titled “Flirting with Images and Commodities” that maps our ephemeral encounters with the contemporary visual and material conditions.
Authors: Bhattacharya Sreedeep 
Published: September 2018

The article addresses how popular imageries of ideal body types and their circulation inspires the construction of similar body ideals to be achieved through body work, body care and body control. While demonstrating a composite relationship between the ‘image’ and the ‘body’, it renders the interdependency and inseparability of these two entities, capturing the dual process of consuming images of the ideal body and transforming body into images for consumption. The article also advances a theoretical model of image–body unification in contemporary India. Citing a wide range of visual representations of the body/image, the article illustrates how the imageries of the ideal body type are often negotiated through body work, and how the worked-out body is then converted to similar body-image for circulation, thereby creating replicas of predominant ideal types and inspiring the production of bodies and images that are identical to that type. The article situates such practices of image production, circulation and emulation within the larger context of greater levels of tolerance, acceptance and dissemination of the eroticised body. It is argued that the acceptance of the eroticised body as lifestyle choice is an integral part of a larger global visual trend. The erosion of the stigma against representation of the body as a legitimate site of pleasure determines our temporal identities by inviting us to participate in the articulation of the desiring self through image-conscious bodies and through images that make the body more desirable.
Microbial erosion assessment on waterlogged archaeological woods (WAWs) from a Chinese ancient shipwreck, Nanhai No. 1
Authors: Gao Mengge , Zhang Qinfen , Feng Xinxin , Chen Dong , Sun Jian , Cui Yong , Xu Runlin 
Published: September 2018

Abstract
This article aims at identifying the timber species of Nanhai No. 1, an ancient shipwreck (Song Dynasty, 800 years ago) sunk in the South China Sea, finding out the types of bacteria that corrode the wood components of the shipwreck and analyzing the erosion level. The changes of the internal structure of wood under different erosion degrees are described as well. With light microscope and electron microscope technology, the microscopic and submicroscopic structures of 20 wooden components from the shipwreck were investigated. The shipwreck was built of 6 timber species, including Pinus massoniana, Fokienia hodginsii, Terminalia hainanensis, Mischocarpus oppositifolius, Alnus trabeculosa and Mussaendopsis beccariana. Based on analyzing the traces left after bacterial erosion, it was determined that the erosive microbes in the sunken ship were mainly erosion bacteria and tunneling bacteria. According to the criteria from relevant literature, the analysis on the bacterial erosion degree of each wood sample shows that most of the wood components present weak to moderate erosion, except a few samples which are totally degraded. Comparison with the bacterial erosion on the wooden components of different ancient shipwrecks, sunk in different years in the southeast China, it is noticed that due to the different seabed environments (sunk conditions), the wood structure of the Nanhai No. 1 sunken ship is the best preserved although its sunken time is longer than the others. The silt-covered anaerobic environment could be one of the protective measures which preserves the ancient sunken ships.
Comparative RNA-Seq analysis on the regulation of cucumber sex differentiation under different ratios of blue and red light
Authors: Song Jiali , Zhang Yiting , Song Shiwei , Su Wei , Chen Riyuan , Sun Guangwen , Hao Yanwei , Liu Houcheng 
Published: September 2018

AbstractCucumber (Cucumis sativus L.) is a typical monoecism vegetable with individual male and female flowers, which has been used as a plant model for sex determination. It is well known that light is one of the most important environmental stimuli, which control the timing of the transition from vegetative growth to reproductive development. However, whether light controls sex determination remains elusive. To unravel this problem, we performed high-throughput RNA-Seq analyses, which compared the transcriptomes of shoot apices between R2B1(Red light:Blue light = 2:1)-treated and R4B1(Red light:Blue light = 4:1)-treated cucumber seedlings. Results showed that the higher proportion of blue light in the R2B1 treatment significantly induced the formation of female flowers and accelerated female flowering time in this whole study. The genes related to flowering time, such as flowering locus T (FT) and SUPPRESSOR OF OVEREXPRESSION OF CO1 (SOC1), were up-regulated after R2B1 treatment. Furthermore, the transcriptome analysis showed that up-regulation and down-regulation of specific DEGs (the differentially expressed genes) were primarily the result of plant hormone signal transduction after treatments. The specific DEGs related with auxin formed the highest percentage of DEGs in the plant hormone signal transduction. In addition, the expression levels of transcription factors also changed after R2B1 treatment. Thus, sex differentiation affected by light quality might be induced by plant hormone signal transduction and transcription factors. These results provide a theoretical basis for further investigation of the regulatory mechanism of female flower formation under different light qualities in cucumber seedlings.
Efficacy and Tolerability of First-Line Cetuximab Plus Leucovorin, Fluorouracil, and Oxaliplatin (FOLFOX-4) Versus FOLFOX-4 in Patients With RAS Wild-Type Metastatic Colorectal Cancer: The Open-Label, Randomized, Phase III TAILOR Trial
Authors: Qin Shukui , Li Jin , Wang Liwei , Xu Jianming , Cheng Ying , Bai Yuxian , Li Wei , Xu Nong , Lin Li-zhu , Wu Qiong , Li Yunfeng , Yang Jianwei , Pan Hongming , Ouyang Xuenong , Qiu Wensheng , Wu Kaichun , Xiong Jianping , Dai Guanghai , Liang Houjie , Hu Chunhong , Zhang Jun , Tao Min , Yao Qiang , Wang Junyuan , Chen Jiongjie , Eggleton S. Peter , Liu Tianshu 
Published: September 2018

Purpose Cetuximab in combination with chemotherapy is a standard-of-care first-line treatment regimen for patients with RAS wild-type (wt) metastatic colorectal cancer (mCRC); however, the efficacy of cetuximab plus leucovorin, fluorouracil, and oxaliplatin (FOLFOX) has never before been proven in a controlled and randomized phase III trial. To our knowledge, the TAILOR trial ( ClinicalTrials.gov identifier: NCT01228734) is the first randomized, multicenter, phase III study of the addition of cetuximab to first-line FOLFOX prospectively choosing a RAS wt population and thus providing confirmative data for the efficacy and safety of cetuximab plus FOLFOX versus FOLFOX alone. Patients and Methods TAILOR is an open-label, randomized (1:1), multicenter, phase III trial in patients from China comparing FOLFOX-4 with or without cetuximab in RAS wt ( KRAS/ NRAS, exons 2 to 4) mCRC. The primary end point of TAILOR was progression-free survival time; secondary end points included overall survival time, overall response rate, and safety and tolerability. Results In the modified intent-to-treat population of 393 patients with RAS wt mCRC, adding cetuximab to FOLFOX-4 significantly improved the primary end point of progression-free survival time compared with FOLFOX-4 alone (hazard ratio, 0.69; 95% CI, 0.54 to 0.89; P = .004; median, 9.2 v 7.4 months, respectively), as well as the secondary end points of overall survival time (current assessment after 300 events: hazard ratio, 0.76; 95% CI, 0.61 to 0.96; P = .02; median, 20.7 v 17.8 months, respectively) and overall response rate (odds ratio, 2.41; 95% CI, 1.61 to 3.61; P < .001; 61.1% v 39.5%, respectively). Treatment was well tolerated, and there were no new or unexpected safety findings. Conclusion The TAILOR study met all of its objectives and relevant clinical end points, confirming cetuximab in combination with FOLFOX as an effective standard-of-care first-line treatment regimen for patients with RAS wt mCRC.
The soluble transhydrogenase UdhA affecting the glutamate-dependent acid resistance system of Escherichia coli under acetate stress
Authors: Zhao Hanjun , Zhou Feng , Xing Quan , Cao Zhengyu , Liu Jie , Zhu Guoping 
Published: September 2018

ABSTRACT
The soluble transhydrogenase (UdhA) is one of two transhydrogenases that play a role in maintaining the balance between NAD(H) pools and NADP(H) pools in Escherichia coli. Although UdhA has been extensively used in metabolic engineering and biocatalysis for cofactor regeneration, its role in acid resistance has not been reported. Here we used DNA microarray to explore the impact of UdhA on transcript levels. We demonstrated that during growth on acetate, the expression of genes involved in the respiratory chain and Gad acid resistance system was inhibited in the udhA-knockout strain. The deletion of udhA significantly repressed the expression of six genes (gadA, gadB, gadC, gadE, hdeA and hdeB) which are involved in Gad acid resistance and resulted in low survival of the bacterium at a low pH of 4.9. Moreover, UdhA was essential for NADH production which is important for the adaptive growth of E. coli on acetate, while NADH concentration in the udhA-knockout strain was quite low and supplemental NADH significantly increased the expression of acid resistance genes and survival of the udhA-knockout strain. These results demonstrated that UdhA is an important source of NADH of E. coli growth on acetate and affects Gad acid resistance system under acetate stress.
The effect of chronic and acute stressors, and their interaction, on testes function: an experimental test during testicular recrudescence
Authors: Abolins-Abols Mikus , Hanauer Rachel E. , Rosvall Kimberly A. , Peterson Mark P. , Ketterson Ellen D. 
Published: September 2018

ABSTRACT
Organisms are expected to invest less in reproduction in response to a stressor, but theory predicts that this effect should depend on the frequency and duration of stressors in the environment. Here, we investigated how an acute stressor affected testes function in a songbird, and how chronic stressors influenced the acute stress response. We exposed male dark-eyed juncos (Junco hyemalis) either to chronic or minimal (control) disturbance during testicular recrudescence, after which we measured baseline testosterone, testosterone after an acute handling stressor, and capacity to produce testosterone after hormonal stimulation. In a 2×2 design, we then killed males from the two chronic treatment groups either immediately or after an acute stressor to investigate the effect of long- and short-term stressors on the testicular transcriptome. We found that chronically disturbed birds had marginally lower baseline testosterone. The acute stressor suppressed testosterone in control birds, but not in the chronic disturbance group. The ability to elevate testosterone did not differ between the chronic treatments. Surprisingly, chronic disturbance had a weak effect on the testicular transcriptome, and did not affect the transcriptomic response to the acute stressor. The acute stressor, on the other hand, upregulated the cellular stress response and affected expression of genes associated with hormonal stress response. Overall, we show that testicular function is sensitive to acute stressors but surprisingly robust to long-term stressors, and that chronic disturbance attenuates the decrease in testosterone in response to an acute stressor.
A systematic performance evaluation of clustering methods for single-cell RNA-seq data
Authors: Duò Angelo , Robinson Mark D. , Soneson Charlotte 
Published: September 2018

Subpopulation identification, usually via some form of unsupervised clustering, is a fundamental step in the analysis of many single-cell RNA-seq data sets. This has motivated the development and application of a broad range of clustering methods, based on various underlying algorithms. Here, we provide a systematic and extensible performance evaluation of 14 clustering algorithms implemented in R, including both methods developed explicitly for scRNA-seq data and more general-purpose methods. The methods were evaluated using nine publicly available scRNA-seq data sets as well as three simulations with varying degree of cluster separability. The same feature selection approaches were used for all methods, allowing us to focus on the investigation of the performance of the clustering algorithms themselves. We evaluated the ability of recovering known subpopulations, the stability and the run time and scalability of the methods. Additionally, we investigated whether the performance could be improved by generating consensus partitions from multiple individual clustering methods. We found substantial differences in the performance, run time and stability between the methods, with SC3 and Seurat showing the most favorable results. Additionally, we found that consensus clustering typically did not improve the performance compared to the best of the combined methods, but that several of the top-performing methods already perform some type of consensus clustering. All the code used for the evaluation is available on GitHub (https://github.com/markrobinsonuzh/scRNAseq_clustering_comparison). In addition, an R package providing access to data and clustering results, thereby facilitating inclusion of new methods and data sets, is available from Bioconductor (https://bioconductor.org/packages/DuoClustering2018).
Portfolio Optimization with Nonparametric Value at Risk: A Block Coordinate Descent Method
Authors: Cui Xueting , Sun Xiaoling , Zhu Shushang , Jiang Rujun , Li Duan 
Published: September 2018

In this paper, we investigate a portfolio optimization methodology using nonparametric value at risk (VaR). In particular, we adopt kernel VaR and quadratic VaR as risk measures. As the resulting models are nonconvex and nonsmooth optimization problems, albeit with some special structures, we propose some specially devised block coordinate descent (BCD) methods for finding approximate or local optimal solutions. Computational results show that the BCD methods are efficient for finding local solutions with good quality and they compare favorably with the branch-and-bound method-based global optimal solution procedures. From the simulation test and empirical analysis that we carry out, we are able to conclude that the mean-VaR models using kernel VaR and quadratic VaR are more robust compared to those using historical VaR or parametric VaR under the normal distribution assumption, especially when the information of the return distribution is limited. The online supplement is available at https://doi.org/10.1287/ijoc.2017.0793 .
Antenatal Hospitalization Among U.S. Women With Intellectual and Developmental Disabilities: A Retrospective Cohort Study
Authors: Mitra Monika , Parish Susan L. , Clements Karen M. , Zhang Jianying , Simas Tiffany A. Moore 
Published: September 2018

Abstract
This population-based retrospective cohort study examines the prevalence of hospital utilization during pregnancy and the primary reason for antenatal hospital utilization among women with intellectual and developmental disabilities (IDD). Massachusetts residents with in-state deliveries that were ≥ 20 weeks gestational age were included via data from the 2002-2009 Massachusetts Pregnancy to Early Life Longitudinal Data System. Among women with IDD, 54.8% had at least one emergency department (ED) visit during pregnancy, compared to 23% of women without IDD. Women with IDD were more likely to have an antenatal ED visit, observational stays, and non-delivery hospital stays. This study highlights the need for further understanding of the health care needs of women with IDD during pregnancy.
Employment, Income, and SSI Effects of Postsecondary Education for People With Intellectual Disability
Authors: Sannicandro Thomas , Parish Susan L. , Fournier Steve , Mitra Monika , Paiewonsky Maria 
Published: September 2018

Abstract
The low employment rates of individuals with intellectual disability (ID) are a major concern. This study examined the effect of postsecondary education on employment atrnd earnings for individuals with ID and the effect of state variation on those outcomes. Rehabilitation Services Administration 911 (RSA-911) files for 2008–2013 were analyzed (n = 11,280 individuals with ID). Multilevel modeling techniques were used to understand state variation, such as various economic and programmatic features that influence outcomes. Postsecondary education was associated with increased employment, increased weekly earnings, decreased reliance on Supplemental Security Income (SSI) benefits. Policy implications are discussed.
Improvement of HER2I655V TARMS-PCR Performance by DNA Quality Analysis
Authors: Budiarto Bugi Ratno , Azamris Azamris , Desriani Desriani 
Published: September 2018

Reliable TARMS-PCR is a prerequisite in constructing a solid conclusion in genetic diagnostics. The validity of data generated by this molecular technique is hampered by a false positive result. In attempt to develop a TARMS-PCR for HER2I655V genotyping with no interfering of bias we used DNase I to eliminate DNA contaminant resided in PCR reagent. TARMS-PCR without enzyme treatment using recombinant plasmids that contained HER2I655V gene with represented its alleles was used to evaluate the presence of false positive result while DNase I treated-PCR reagent was used in TARMS-PCR to evaluate the effective dose of the enzyme and further to adjust the TARMS-PCR conditions. PCR master mix kit used in this study produced a false positive result on HER2I655V TARMS-PCR as proven by the presence of multiple PCR products in Non-Template Control (NTC) and 0.1 U of the enzyme could eliminate this DNA contaminant effectively, although this pretreatment altered the specificity of HER2I655V TARMS-PCR genotyping on certain genotype. Combination of touchdown TARMS-PCR with another allele-specific primer recovered specificity of detection on this model system. Interestingly, this optimized HER2I655V TARMS-PCR can only be used for genotyping the clinical samples if only further optimization was done using genomic DNA as template
<i>ILE655VAL</i> Genotyping Study of HER2 - Positive Breast Cancer of Patients from Padang - Indonesia With High Resolution Melting Technique
Authors: Wulandari Dwi , Azamris Azamris , Nurhayati Isna , Warisman M Ali , Budiarto Bugi Ratno , Desriani Desriani 
Published: September 2018

Transtuzumab has proven to be a great improvement in the treatment of HER2 + of breast cancer patients, but it is associated with relevant adverse cardiac events and with significantly elevated cost of treatment. One of the risk factors for cardiotoxicity due to transtuzumab is the I655V HER2 polymorphism (GTC> ATC mutation) in which the allele mutant (Ile val or val / val) has a higher risk than the wild type (Ile/Ile). The detection of specific alleles is very important for therapeutic decision-making. In this study, our group have developed a HRM with EvaGreen dye method to discriminate specific allele of I655V HER2 (wild type, heterozygote mutant or homozygote mutant) in 66 frozen section samples of HER2+ of breast cancer patients. Our group reported the wild type is the most prevalent allele (77,27%), whereas heterozygous mutation is significantly present in this research (21,21%) and only 1,52% of sample detected as minor allele. It showed that only one sample detected as a minor allele (val/val) and may have relatively low abundance in the population. This method has been compared with sanger sequencing and giving 100% of validity.