bluuebunny/crossref_metadata_2025_split huggingface dataset:
“RIG-I mediated neuron-specific IFN type 1 signaling in FUS-ALS induces neurodegeneration and offers new biomarker-driven individualized treatment options for (FUS-)ALS.”
Authors: Naumann Marcel , Kretschmer Stefanie , Dash Banaja , Peikert Kevin , Glaß Hannes , Großmann Dajana , Günther René , Petri Susanne , Rödiger Annekathrin , Brenner David , Pan-Montojo Francisco , Aronica Eleonora , Kipp Markus , Zimyanin Vitaly , Sterneckert Jared , Grehl Torsten , Böckers Tobias M. , Oeckl Patrick , Lee-Kirsch Min Ae , Hermann Andreas 
Published: December 2024

AbstractRecent research has demonstrated significant aberrant activation of the innate immune system in ALS model systems due to mutations in SOD1, TARDBP and C9orf72 through stimulation of the TBK1-IRF3 pathway. This pathway can be activated, for example, by cGAS-STING-dependent sensing of cytosolic DNA that accumulates as a result of chronic DNA damage and defective mitochondria, both of which have been identified as early pathology in FUS-ALS spinal motor neurons (sMNs). Therefore, we analysed innate immune pathways in isogenic and non-isogenic FUSmutiPSC-derived sMNs, which revealed upregulation of interferon-stimulated genes (ISGs) and activation of the TBK1-IRF3 pathway in FUSmutsMNs. Notably, we found evidence for accumulation of cytosolic dsRNA and its sensor RIG-I in FUS-ALS. RIG-I, but not MDA5, was found to be significantly upregulated in FUSmutsMNs, and siRNA-mediated knockdown abolished the increased IFN1 activation in FUSmutsMNs. In post-mortem analysis, RIG-I was highly expressed in the remaining α-MNs. IFN treatment of FUSwtsMNs phenocopied the axonal degeneration of FUSmutsMNs. Mechanistically, DNA damage induction did not increase ISG expression, but dsRNA was increased in the mitochondria of FUSmutsMNs. Mitochondrial transcription, a known source of dsRNA, was found to be upregulated in compartmental axonal RNAseq analysis and its inhibition reduced ISGs in FUS-ALS sMNs. Furthermore, the JAK-STAT inhibitor ruxolitinib alleviated the upregulated ISG expression and reversed the axonal degeneration of sMNs. Finally, we analysed ISG expression in peripheral blood samples from 18 FUS-ALS patients, eight of whom had a significantly elevated interferon signature. Blood ISGs correlated with disease progression rate and negatively with disease duration. RIG-I-mediated innate immune activation in sMNs may be an interesting novel individualised biomarker-driven therapeutic target in (FUS-) ALS.A one-sentence summary of your paperRIG-I-I-mediated innate immune activation is found in FUS-ALS spinal motor neurons caused by cytosolic dsRNA accumulation due to mitochondrial transcriptional activation and is amenable to JAK-STAT inhibition and might thus be an interesting novel individualized biomarker-driven therapeutic approach in (FUS-) ALS
Cyanoexosortase B is essential for motility, biofilm formation and scytonemin production in a filamentous cyanobacterium
Authors: Parrett Gabriel A. , Haft Daniel H. , Ruiz Maida , Garcia-Pichel Ferran , Ebmeier Christopher C. , Risser Douglas D. 
Published: December 2024

AbstractExosortases are involved in trafficking proteins containing PEP-CTERM domains to the exterior of gram-negative bacterial cells. The role of these proteins in cyanobacteria, where such homologs are common, has not been defined. The filamentous cyanobacteriumNostoc punctiformecontains a single putative exosortase, designated cyanoexosortase B (CrtB), implicated by previous work in both the motility, and in the production of the UV-absorbing pigment, scytonemin. To determine the role ofcrtBinN. punctiforme, acrtB-deletion strain (ΔcrtB) was generated. ΔcrtBpresented the loss of motility, biofilm formation, and scytonemin production. In the case of motility, the ΔcrtBmutant exhibited a specific defect in the ability of hormogonia (specialized motile filaments) to adhere to hormogonium polysaccharide (HPS) and several PEP-CTERM proteins expressed in motile hormogonia were differentially abundant in the exoproteome of the wild type compared to the ΔcrtBstrain. These results are consistent with the hypothetical role of CrtB in the processing and export of PEP-CTERM proteins that play a critical role in stabilizing the interaction between the filament surface and HPS to facilitate motility and biofilm formation. In the case of scytonemin, the late biosynthetic steps of which occur in the periplasm and whose operon contains several putative PEP-CTERM proteins, ΔcrtBfailed to produce it. Given the abundance of putative PEP-CTERM proteins encoded in theN. punctiformegenome, and the fact that this study only associates a fraction of them with biological functions, it seems likely that CrtB may play an important role in other biological processes in cyanobacteria.ImportanceIn gram-negative bacteria, exosortases facilitate the trafficking of proteins to the exterior of the cell where they have been implicated in stabilizing the association of extracellular polymeric substances (EPS) with the cell surface to facilitate biofilm formation and flocculation, but the role of exosortases in cyanobacteria has not been explored. Here, we characterize the role of cyanoexosortase B (CrtB) in the filamentous cyanobacteriumNostoc punctiforme, demonstrating thatcrtBis essential for motility, biofilm formation, and the production of the sunscreen pigment scytonemin. These findings have important implications for understanding motility and biofilm formation in filamentous cyanobacteria as well as efforts toward heterologous production of scytonemin in non-native hosts.
Single-nuclei sequencing ofMoricandia arvensisreveals bundle sheath cell function in the photorespiratory shuttle of C3-C4intermediate Brassicaceae
Authors: Triesch Sebastian , Reichel-Deland Vanessa , Martín José Miguel Valderrama , Melzer Michael , Schlüter Urte , Weber Andreas P.M. 
Published: December 2024

AbstractSpatially confined gene expression determines cell identity and is fundamental to complex plant traits. In the evolutionary transition from C3to the more efficient C4photosynthesis, restricting the glycine decarboxylase reaction to bundle sheath cells initiates a carbon concentrating mechanism via the photorespiratory glycine shuttle. This evolutionary step is generally thought to play an essential role in the progression from ancestral C3to C4photosynthesis. Plants operating this shuttle are often referred to as C3-C4intermediates or C2species. Within the Brassicaceae family, which includes model plants and crops, such species have evolved independently at least five times. However, research on the biochemistry of C3-C4intermediates in the Brassicaceae has been limited to a few case studies of differentially localized proteins between mesophyll and bundle sheath cells. Here, we leveraged recent advances in single-cell transcriptome sequencing to better understand how new cellular specialization affects interconnected pathways. We generated a single-nuclei RNA sequencing dataset forMoricandia arvensis, a Brassicaceae with C3-C4intermediate characteristics, and compared it to a publicly available single-cell transcriptome of leaf tissue of the C3Arabidopsis thaliana. We independently confirmed the localization of selected photorespiratory proteins by electron microscopy of immunogold-labelled leaf sections. Our analysis revealed the shift in expression of genes directly associated with the photorespiratory glycine decarboxylase reaction, but also of related pathways, such as ammonium assimilation, synthesis of specific amino acids, redox regulation, and transport to theM. arvensisbundle sheath. In contrast, the expression of these genes was not restricted to this cell type in the C3plant.HighlightSingle-nucleus RNA sequencing ofMoricandia arvensisreveals bundle sheath cell-specific expression of photorespiration genes and associated pathways beyond glycine decarboxylase, including ammonium assimilation and redox regulation. This highlights the key role of metabolic compartmentalization in supporting C3-C4intermediate photosynthesis.
Polycomb Repressive Complex 2 promotes atherosclerotic plaque vulnerability
Authors: Joshi Divyesh , Chakraborty Raja , Bhogale Tejas , Furtado Jessica , Deng Hanqiang , Traylor James G. , Orr Anthony Wayne , Martin Kathleen A. , Schwartz Martin A. 
Published: December 2024

AbstractKey findings:1. PRC2 regulates EC shear stress responses.2. PRC2 governs Klf2/4 suppression downstream of Pcdhg.3. High PRC2 in ASCVD-prone arterial regions suppresses Klf2/4 to promote ASCVD.4. Athero-protective Klf2/4 induction upon PRC2 inhibition requires Notch signaling.5. Tazemetostat, an FDA approved PRC2 inhibitor, slows ASCVD progression and improves markers of plaque stability.Atherosclerotic cardiovascular disease (ASCVD), the leading cause of mortality worldwide, is driven by endothelial cell inflammatory activation and counter-balanced by anti-inflammatory transcription factors Klf2 and Klf4 (Klf2/4). Understanding vascular endothelial inflammation to develop effective treatments is thus essential. Here, we identify, Polycomb Repressive Complex (PRC) 2, which blocks gene transcription by trimethylating histone3 Lysine27 in gene promoter/enhancers, as a potent, therapeutically targetable determinant of vascular inflammation and ASCVD progression. Bioinformatics identified PRC2 as a direct suppressor of Klf2/4 transcription. Klf2/4 transcription requires Notch signaling, which reverses PRC2 modification of Klf2/4 promoter/enhancers. PRC2 activity is elevated in human ASCVD endothelium. Treating mice with established ASCVD with tazemetostat, an FDA approved pharmacological inhibitor of PRC2, slowed plaque progression by 50% and drastically improved markers of plaque stability. This study elucidates a fundamental mechanism of vascular inflammation, thus identifying a potential method for treating ASCVD and possibly other vascular inflammatory diseases.
Structural Insights into Competitive Binding Dynamics between RALF23/33 and PCP-B in Brassicaceae Pollination
Authors: Bhalla Hemal , Sudarsanam Karthik , Srivastava Ashutosh , Sankaranarayanan Subramanian 
Published: December 2024

AbstractEnsuring successful fertilization, viable offspring production, genetic isolation, and maintaining species integrity is pivotal for the survival of flowering plants. Members of Brassicaceae employ a “gatekeeping mechanism” involving interaction between stigmatic membrane-boundCatharanthus roseusreceptor-like kinase 1-like (CrRLK1L) receptor, FERONIA, GPI anchored protein LLG2 (LORELEI-LIKE GLYCOPHOSPHATIDYLINOSITOL-ANCHORED PROTEIN 2) and autocrine secreted RALF23/33 (Rapid alkalinization factor) peptide. This binding establishes a barrier for pollen hydration by inducing ROS (Reactive Oxygen Species). Conversely, in the presence of compatible pollen, paracrine-secreted cysteine-rich peptides such as PCP-Bγ compete with RALF23/33 for binding to the FERONIA-LLG2 complex, thus reducing ROS levels, ensuring successful pollen hydration and germination. Despite its crucial role, the structural basis of this competitive binding dynamics remains elusive owing to the lack of structural data and the inherent flexibility of these peptides. Using structural modeling, molecular docking, and simulations, this study reveals that PCP-Bγ binds to the same negatively charged pocket in the FERONIA-LLG2 complex as RALF23, displacing and interrupting the heterodimerized structure, thus reducing ROS levels to promote pollination. Our study unveils the experimental data-based predicted models, competitive binding dynamics, and mechanism behind this “gatekeeping mechanism,” shedding light on the molecular mechanism underlying this pollen hydration barrier in Brassicaceae.
Anisotropy of the zigzag order in the Kitaev honeycomb magnet
α−RuBr3
Authors: Pearce John S. , Kaib David A. S. , Ma Zeyu , Ni Danrui , Cava R. J. , Valentí Roser , Coldea Radu , Coldea Amalia I. 
Published: December 2024

Kitaev materials often order magnetically at low temperatures due to the presence of non-Kitaev interactions. Torque magnetometry is a very sensitive technique for probing the magnetic anisotropy, which is critical in understanding the magnetic ground state. In this paper, we report detailed single-crystal torque measurements in the proposed Kitaev candidate honeycomb magnet α−RuBr3, which displays zigzag order below 34 K. Based on angular-dependent torque studies in magnetic fields up to 16 T rotated in the plane normal to the honeycomb layers, we find an easy-plane anisotropy with a temperature dependence of the torque amplitude following closely the behavior of the powder magnetic susceptibility. The torque for the field rotated in the honeycomb plane has a clear sixfold periodicity with a sawtooth shape, reflecting the threefold symmetry of the crystal structure and stabilization of different zigzag domains depending on the field orientation, with a torque amplitude that follows an order parameter form inside the zigzag phase. By comparing experimental data with theoretical calculations we highlight the importance of relevant anisotropic interactions and the role of the competition between different zigzag domains in this candidate Kitaev magnet.

Published by the American Physical Society
2024
Deep learning-based recognition of construction activities in real construction site environment
Authors: Karatas Ibrahim , Budak Abdulkadir 
Published: December 2024

PurposeToday’s technological advancements have had a significant impact on the construction industry. Managing and controlling complex construction projects has been made significantly easier using technological tools. One such advancement is the automatic identification of workers’ activities. This study aims to classify construction worker activities by analyzing real-time motion data collected from sensors.Design/methodology/approachIn accordance with our specific goals, we utilized advanced deep-learning methodologies such as deep neural networks, convolutional neural network, long short-term memory and convolutional long short-term memory to analyze the data thoroughly. This involved experimenting with various window sizes and overlap ratios to determine the optimal combination that would result in the most accurate predictions.FindingsBased on the analysis results, the convolutional long short-term memory (ConvLSTM) deep learning model with a window size of 4.8 s and an overlap rate of 75% was found to be the most accurate prediction model. This model correctly predicted 98.64% of the basic construction worker activities in a real construction site environment.Originality/valuePrevious studies have mainly been conducted in laboratory environments and have focused on basic construction activities such as lifting, moving, sawing and hammering. However, this study collected data from real workers in a real construction site environment. Various deep learning models were employed to determine the most accurate one. Additionally, several options were tested to determine the optimal window size and overlap ratio during the data segmentation phase, aiming to select the most suitable ones for preparing the data for the model.
Finding the sweet spot: effects of exporting on the relationship between R&D investment and NPD performance
Authors: Sousa Carlos M.P. , Tsinopoulos Christos , Yan Ji , Benito Gabriel R.G. 
Published: December 2024

PurposeThe aim of this research is twofold: (1) to investigate when the effect of R&D investment on New Product Development (NPD) performance peaks – the sweet spot and (2) to analyze the influence of firms’ export activities on where that spot is. Drawing on the knowledge-based view (KBV), we argue that export intensity and export experience lead to differential effects on how R&D investments are converted into new products.Design/methodology/approachWe test our conceptual framework using time lagged data and optimal-level analysis. The dataset consists of an unbalanced panel of 608,891 observations and 333,516 firms.FindingsThe results support the expected inverted U-shaped relationship between R&D investment and NPD performance. They also show moderating effects of export intensity and experience. Export intensity enhances innovation processes by enabling firms to stretch the points at which R&D investments eventually taper off. In contrast, export experience improves firms’ ability to convert R&D investments into NPD performance. Our results demonstrate that, all else equal, firms with relatively higher export experience can spend less on R&D and still achieve higher levels of NPD performance.Originality/valueWe contribute to the literature by investigating how export activities provide a valuable context for understanding the theoretical mechanisms that help explain the inverted U-shaped relationship between R&D investment and innovation. We show the effects of exporting activities on the precise points where the R&D investment–NPD performance relationship peaks, thereby identifying the optimal point within this nonlinear relationship.
Are private banks more sensitive to changes in reserve requirements? Evidence from an emerging market
Authors: Swamy Vighneswara , Narayanamurthy Vijayakumar 
Published: December 2024

PurposeThis article explores the effects of monetary policy rates and interest rate structures on bank profitability.Design/methodology/approachWe studied 65 Indian commercial banks over time, including economic cycles, consolidation and the Great Financial Crisis. We categorized commercial banks by ownership (public, private or foreign) and predicted how they will react to monetary policy changes. We employed the instrumental variable estimate approach and panel Granger causality tests to give evidence of the direction of causation in the monetary policy and bank performance nexus.FindingsPrivate and international banks, we believe, are more sensitive to changes in reserve requirements because they are more effective at maintaining statutory reserves. Private and international banks are more susceptible to repo rate fluctuations than state banks. In contrast, public banks are more sensitive to bank rates because they are more likely than private and international banks to use the bank rate window of accommodation.Originality/valueWe studied the impact of monetary policy rates on bank performance within the banking-dominated financial system of an emerging economy – a focus that has not been previously explored. There has been little research into the connection between monetary policy rates and bank performance in emerging markets, notably in India.
Toward an understanding of the robot rights spectrum: an integrative framework from an Islamic economics perspective
Authors: Mohd Thas Thaker Mohamed Asmy Bin , Akbar Mohamed Aslam , Imon Riasat Amin Amin 
Published: December 2024

Purpose
This paper aims to explore the role and relationship of ethics and morals in technology, specifically examining how Islam, as a religion that emphasizes spirituality and sacredness, can uniquely influence the concept of robot rights.

Design/methodology/approach
The existing literature on robot rights and Islamic perspectives has been critically reviewed to address the study’s objectives.

Findings
In Islam, robots are viewed similarly to property ownership, where the owner holds responsibilities rather than absolute control. Islamic ownership rights are distinct compared to conventional ownership models. In Islam, private ownership is limited, as God is considered the ultimate owner of all assets. Assets, including robots, must be managed according to Islamic values and ethics. Unlike conventional ownership, where the owner can dispose of their property without justification, Islamic principles grant more rights to assets (including robots). This difference arises from the sacred origins of economic resources in Islam, which extends to the treatment of assets as inputs in an economy. Therefore, spirituality, as defined in Islam, uniquely influences the rights of robots.

Originality/value
As robotics becomes an increasingly significant part of our lives, religion plays a growing role in shaping the ethical and moral framework within which robots operate. This study is among the first to present an integrative framework and evaluate robot rights from an Islamic economics perspective.
The cross-domain impact of home stressors on job performance through leisure crafting
Authors: Chen I-Shuo 
Published: December 2024

PurposeWe explored the mechanism underlying the associations between different home stressors and job performance. We investigated whether challenge and hindrance home stressors are related to leisure crafting and thereby to job performance. We also examined whether key resources amplify the positive association between leisure crafting and job performance.Design/methodology/approachWe conducted a three-wave time lag study to investigate a group of employees (n = 534) in Taiwan. We evaluated challenge/hindrance home stressors and key resources at Time 1, leisure crafting one week later, i.e. at Time 2 and manager-rated job performance an additional three months later, i.e. at Time 3.FindingsChallenge home stressors were associated with improved leisure crafting, thereby benefiting job performance; hindrance home stressors had the opposite effect. Additionally, openness to experience strengthened the positive association between leisure crafting and job performance.Practical implicationsOrganizations may support employees’ efforts to promote/mitigate challenge/hindrance home stressors. They may also encourage employees to be open to life experiences that can amplify the contributions of leisure crafting to job performance.Originality/valueThe questions of whether and how home stressors are related to individuals’ work remain controversial in the literature. We offer new insights into how these stressors can contribute to or impede job performance, thereby extending our knowledge of the role of home stressors in job performance.
Curricular Self‐management for patients on ventricular assist device support decreases depression: A multicenter randomized controlled trial
Authors: Kugler Christiane , Spielmann Hannah , Richter Fabian , Lauenroth Volker , Semmig‐Könze Sandra , Spitz‐Köberich Christine , Bertsche Tim , Staus Paulina , Weber Susanne , Albert Wolfgang , Tigges‐Limmer Katharina ,   the Selma Study Group
Published: December 2024

AbstractBackgroundUtilization of ventricular assist device (VAD) support in patients with end‐stage heart failure is well advanced. Recent studies emphasize the need to develop and evaluate evidence‐based psychosocial support interventions for patients following VAD implantation.MethodsA national, multi‐center study in four established German heart centers was initiated. An evidence‐based VAD curriculum was developed; a randomized controlled trial evaluated the impact of the interprofessional intervention over time. Primary combined endpoint was the occurrence of adverse events (thromboembolic events, driveline infections, bleeding, rehospitalization, and death); secondary endpoints were psychosocial outcomes (anxiety, depression [HADS], quality of life [QoLVAD], social support [FSoZu], and self‐management [SELMA]). A total of 140 patients were randomized block‐wise to intervention (IG n = 70) or control (CG n = 70). Center‐effects were considered. Instruments were completed at four time points (T0‐T3).ResultsAt baseline, intervention group (IG) patients were 19% female (control group [CG] 19%; p = 0.982); 58 ± 11 years. (CG 58 ± 11 years.; p = 0.966); 80% were implanted electively (CG 79%; p = 0.968). No significant difference in primary endpoint was found between IG versus CG (p > 0.05). For secondary endpoints, mixed linear regression revealed a significant reduction in depression scores in IG compared to CG (est. 1.18; 95% CI −2.17 to −0.18; p = 0.021). Clinically significant reductions in anxiety were greater in IG patients between T0 and T3 (IG 51.9%; CG 40.7%); increased anxiety was reported in 3.7% IG (11.1% CG). Mixed linear regression analyses favored IG (all >0.05) for QoL, social support, and self‐management.ConclusionA comprehensive curricular VAD intervention has potential to decrease depression levels and improve psychosocial outcomes of patients on VAD support.