bluuebunny/crossref_metadata_2025_split huggingface dataset:
MiR-130c-5p targets the SHVV n gene and upregulates immune cytokines (IL-6, IL-22, IL-1β) to inhibit viral replication
Authors: Wei Jin , Ji Yan , Bai Yaqian , Cheng Rui , Zhang Jiaqi , Hu Xianqin , Zhang Chi 
Published: November 2024

BackgroundSnakehead vesiculovirus (SHVV) has led to huge economic losses in snakehead aquaculture, and its pathogenic mechanisms is still not fully understood. MicroRNAs (miRNAs), as an important class of non-coding RNAs, play a key regulatory role in the process of viral infection.MethodsWe examined the effect of SHVV infection on the expression of miR-130c-5p and the effect of overexpression of miR-130c-5p on the proliferation of SHVV. Cotransfection of viral N protein and miR-130c-5p, and the effect of miR-130c-5p on the expression of N protein was detected. Meanwhile, the effect of overexpression of miR-130c-5p on the expression of various immune factors in the case of viral infection were also tested.ResultsIn this study, SHVV infection significantly upregulated the expression of miR-130c-5p in channel catfish ovary (CCO) cells in a time- and dose-dependent manner. The further research revealed that miR-130c-5p mimic significantly inhibited, while its inhibitors promoted SHVV replication. In addition, miR-130c-5p could directly target the viral mRNA of n gene, and overexpression of miR-130c-5p could significantly decrease, and conversely, downregulation of miR-130c-5p could increase the mRNA and protein expression of the viral n gene. Meanwhile, overexpression of miR-130c-5p also upregulated the expression of immune-related genes, such as nucleotide-oligomerization domain (NOD)-like receptor subfamily C3 (NLRC3), myeloid differentiation factor 88 (MyD88), nuclear factor kappa-B (NF-κB), interleukin-6 (IL-6), interleukin-22 (IL-22), and interleukin-1beta (IL-1β) in host cells.ConclusionmiR-130c-5p was upregulated in the host during SHVV infection, and the upregulated miR-130c-5p directly inhibited viral replication by targeting the n gene of SHVV and promoting viral nucleoprotein degradation. The up-regulated miR-130c-5p also activated the expression of immune-related genes such as NLRC3, MyD88, NF-κB, IL-6, IL-22, and IL-1β, which were involved in the regulation of the signaling pathways including NF-κB, MyD88, Toll-like receptor (TLR), NLR, and janus tyrosine kinase-signal converter and activator of transcription (JAK-STAT), to enhance the host's antiviral immune response, and thus indirectly inhibited the viral proliferation.
Uremic sarcopenia: the role of intramuscular adipose tissue as a potential early identifier
Authors: Noce Annalisa , Ceravolo Maria Josè , Gualtieri Paola , Marrone Giulia , Romano Lorenzo , Shoshi Amir , Di Lauro Manuela , De Lorenzo Antonino 
Published: November 2024

IntroductionSarcopenia is a chronic pathological condition, first defined in 2010 and revised in 2018. The most recent definition of sarcopenia focuses mostly on “low muscle strength.” A secondary form of sarcopenia is represented by uremic sarcopenia (US), a condition that characterizes end-stage kidney disease (ESKD) patients. The intramuscular adipose tissue (IMAT) seems to impact negatively on muscle strength, as it would seem to replace muscle fibers with a non-contractile component. The study aims to compare body composition parameters—both standardized and innovative—related to the diagnosis of US in hemodialysis (HD) patients, stratified by sarcopenia diagnosis. Furthermore, the different indices of sarcopenia are compared in order to evaluate their predictive capacity.MethodsWe analyzed 48 ESKD patients according to the sarcopenia diagnosis, obtained using dual-energy X-ray absorptiometry (DXA). Moreover, we assessed the presence of IMAT and calculated the sarcopenia index (SI).ResultsFor the study, the enrolled population was divided according to the sarcopenia diagnosis: no sarcopenic patients had higher transferrin (p = 0.03), total proteins (p = 0.04), and azotemia pre-dialysis (p = 0.05) values. On the contrary, atherogenic indices were lower in no sarcopenic patients. Moreover, we observed an indirect correlation between the SI and parathyroid hormone (PTH) (p = 0.00138, R2 = 0.54). Finally, we calculated the prevalence of sarcopenia and sarcopenia adjusted for IMAT. We showed a different prevalence between sarcopenia diagnosed with a standard index and an index adjusted for IMAT (p = 0.043). In conclusion, we believe that the most important result obtained is the indirect correlation between SI and PTH. These data corroborate the theories, in which PTH seems to play a central role in the cachexia genesis. Moreover, the SI adjusted for IMAT seems to be a more reliable parameter for the early identification of subjects at risk of developing US, allowing timely implementation of targeted therapeutic strategies.
Diagnostic accuracy of lung ultrasound to predict weaning outcome: a systematic review and meta-analysis
Authors: Zhang Zhiyang , Guo Li , Wang Huawei , Zhang Ze , Shen Limin , Zhao Heling 
Published: November 2024

BackgroundThis systematic review and meta-analysis aim to systematically assess the diagnostic accuracy of lung ultrasound in predicting weaning failure from mechanical ventilation in critically ill patients.MethodsWe searched the relevant literature up to January 2024 in the databases Web of Science, Cochrane Library, Embase, and PubMed. Two researchers independently screened eligible studies and extracted data; disagreements, if any, were resolved through discussion or consultation with a third-party expert. The quality of the included studies was assessed using the Quality Assessment of Diagnostic Accuracy Studies-2 tool. Statistical analyses were performed using Review Manager version 5.3 and Stata version 18.0, applying bivariate random-effects models to estimate sensitivity, specificity, diagnostic odds ratios, and their 95% confidence intervals, as well as to summarize receiver operating characteristic curves. Inter-study heterogeneity was assessed using the I-squared statistic, and potential sources of heterogeneity were explored by meta-regression analysis. The study follows the guidelines for Preferred Reporting Items for Systematic Reviews and Meta-Analyses in reporting.ResultsFourteen studies were included in the systematic review, of which 13 studies (totaling 988 patients) were included in the meta-analysis. The meta-analysis revealed an overall sensitivity of 0.86 (95% confidence interval: 0.77–0.91) and a specificity of 0.75 (95% confidence interval: 0.66–0.83) for lung ultrasound in predicting extubation failure. The area under the receiver operating characteristic curve was 0.87 (95% confidence interval: 0.84–0.89). Meta-regression analysis identified lung ultrasound thresholds, reference standards (extubation outcomes), and study flow and time bias as significant factors influencing diagnostic accuracy.ConclusionThis systematic review and meta-analysis demonstrated that lung ultrasound has high diagnostic accuracy in predicting extubation failure in mechanically ventilated critically ill patients. Despite some study heterogeneity, lung ultrasound proved to be a reliable predictive tool for extubation failure. Future research should focus on standardizing the definition of extubation failure, exploring the impact of different thresholds on the predictive ability of lung ultrasound, and validating its application in various clinical settings to enhance its utility and accuracy in clinical practice.Systematic review registrationThis systematic review and meta-analysis was registered with PROSPERO (registration number: CRD42024555909). The study adhered to the guidelines set by the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). Details of the PROSPERO protocol can be found in Supplementary Table 1.
Anhydrous microwave synthesis as efficient method for obtaining model advanced glycation end-products
Authors: Bronowicka-Szydełko Agnieszka , Madziarska Katarzyna , Kuzan Aleksandra , Lewandowski Łukasz , Adamiec-Mroczek Joanna , Pietkiewicz Jadwiga , Tota Maciej , Ziomek Maciej , Stach Wojciech , Trocha Tymoteusz , Piersiak Marcin , Pachana Maciej , Galińska Zuzanna , Korpacki Andrzej , Dróżdż Olgierd , Matuszyk Janusz , Mitkiewicz Małgorzata , Gamian Andrzej , Gostomska-Pampuch Kinga 
Published: November 2024

IntroductionAdvanced glycation end-products (AGEs) are capable of stimulating oxidative stress and inflammation. This study investigates the synthesis of medium crosslinked AGEs (the most optimal form of AGEs because of soluble in water, used in many assays as markers) and their biochemical properties.MethodsOne of model protein–myoglobin from horse heart muscle (MB) and a chosen respective glycation factor – D-melibiose (mel), acrolein (ACR), D-glucose (glc), 4-hydroksynonenal (4HNE), trans-2-nonenal (T2N), methylglyoxal (MGO) – were subjected to high temperature water synthesis (HTWS) and high temperature microwave synthesis in anhydrous conditions (HTMS). The syntheses were deliberately carried out in two different conditions to check whether adding an additional energy source (microwaves) while lowering the temperature and shortening the reaction time would allow for more effective obtaining of medium-cross-linked AGEs, monitored with SDS-PAGE. Products were analyzed using fluorescence measurements, Enzyme-Linked Immunosorbent Assay (ELISA) and immunoblotting tests and electrophoretic mobility shift assay to evaluate their ability to activate nuclear factor kappa-light-chain-enhancer (NF-κB).ResultsMedium cross-linked AGEs were more efficiently obtained in HTMS. Fluorescence was high for MB-ACR, MB-T2N and MB-glc products. Anti-MAGE antibodies showed reactivity towards MB-mels of HTMS and HTWS, and the MB-4HNEs from HTMS. HTWS products, apart from MB-ACR, did not activate NF-κB, whereas MB-ACR, MB-4HNE, MB-mel, and MB-T2N products of HTMS strongly activated this factor that indicates their strong pro-inflammatory properties.ConclusionHTMS is a fast and efficient method of synthesizing medium cross-linked AGEs.
Exploring motor imagery as a therapeutic intervention for Parkinson’s disease patients: a scoping review
Authors: Michel Maxime , Terragno Elena , Bereau Matthieu , Magnin Eloi , Gueugneau Nicolas , Soares Antonio Vinicius , Sagawa Yoshimasa 
Published: November 2024

BackgroundMotor imagery (MI) has emerged as a promising therapeutic approach for Parkinson’s disease (PD). MI entails mentally rehearsing motor actions without executing them. This cognitive process has garnered attention due to its potential benefits in aiding motor function recovery in patients. The purpose of this review was to highlight the findings observed in motor symptoms, balance, gait, and quality of life.MethodsA literature search was carried out in Medline, Embase, Cochrane, and Physiotherapy Evidence Database (PEDro), from the first publication to February 2024. Studies with at least one keyword to PD and MI in the title were included.ResultsThe analysis included 53 studies out of the 262 identified. These comprised 12 randomized controlled trials (RCTs) with an average PEDro score of 6.6 out of 10, as well as 41 non-RCT studies. Notably, the majority of the RCTs focused on balance, gait, and lower limb exercises. The experimental group found an 85.2% improvement on the Timed Up and Go (TUG) with a cognitive task (p &lt; 0.02), 5.8% improvement on the TUG (p &lt; 0.05), and 5.1% improvement in walking speed (p &lt; 0.05). Other variables did not show significant improvement. In descriptive and non-RCT studies, there were various tasks and outcomes for the lower and upper limbs. It has been demonstrated that there was no difference in execution time in MI between patients and healthy subjects (HS), whereas motor execution was slower in patients. Several tasks were analyzed for the upper limb, including thumb opposition, joystick movements, and writing tasks with variable results. RCTs were more focused on balance, lower limbs, and walking. There was no specific outcome regarding the upper limb or speech. Additionally, the heterogeneity of tasks and outcomes across studies is also a limitation.ConclusionCurrent research on walking disorders in PD shows promise, but further investigations are crucial, particularly with an emphasis on upper limb function and speech. Studies with larger sample sizes and more precise methodologies are needed to enhance our understanding of the potential benefits of MI within the framework of comprehensive PD rehabilitation.
Hemodynamic alterations and their clinical implications in the vertebrobasilar system among patients with isolated posterior circulation ischemic vertigo
Authors: Song Xuhua , Liang Jingwei , Tian Congzhe 
Published: November 2024

BackgroundThis research aimed to ascertain independent risk factors and the diagnostic value of vascular parameters in differentiating posterior circulation ischemic isolated vertigo (PCI-IV) from vestibular peripheral vertigo (VPV).MethodsThis study involved 247 patients with acute-onset vertigo, categorized into two groups: PCI-IV and VPV. Multivariate logistic regression was conducted to pinpoint independent risk factors for PCI-IV.ResultsThe duration of vertigo, particularly episodes lasting more than a few hours, was a significant predictor of PCI-IV (OR = 2.183, p &lt; 0.001). The presence of diabetes mellitus (OR = 1.746, p = 0.008) and hypertension (OR = 2.291, p = 0.004) also notably increased the likelihood of PCI-IV. Hemodynamic measurements such as the inner diameter and average blood flow velocity (Vmean) of the vertebral artery, as well as the resistive index (RI), were identified as significant predictive factors (p ≤ 0.033). The ROC analysis demonstrated the vertebral artery RI had the highest diagnostic accuracy with an area under the curve (AUC) of 0.78, indicating an optimal balance between sensitivity and specificity.ConclusionClinical features such as the duration of vertigo, diabetes mellitus, and hypertension, along with vascular hemodynamics, are crucial in assessing the risk of PCI-IV. The RI in the vertebral artery emerged as a particularly potent diagnostic parameter. These findings advocate a multifaceted diagnostic approach, combining clinical and vascular parameters for the effective identification and management of PCI-IV.
Neural functions vary by return-to-sport status in participants with anterior cruciate ligament reconstruction: a retrospective cohort study using sub-bands of resting-state functional magnetic resonance
Authors: Song Hongyun , Zhu Sunan , Pan Zongyou , Yu XiaoJing , Xiong Bing , Dai Xuesong 
Published: November 2024

ObjectiveThis study aimed to characterize the differences in neural function among patients with different functional abilities 2 years after anterior cruciate ligament reconstruction (ACLR).DesignResting-state functional magnetic resonance imaging was performed to obtain blood-oxygen-level-dependent values for ACLR returned to sports coper participants (CP), non-coper participants (NP), and healthy controls (HC). The amplitude of low-frequency fluctuations (ALFF) and regional homogeneity (ReHo) calculated changes in the standard frequency band (SFB) (0.01–0.08 Hz), Slow4 (0.027–0.073 Hz), and Slow5 (0.01–0.027 Hz). Clinical correlations were investigated.ResultsThe right cerebellum_8 and bilateral putamen in SFB, while the right cerebellum_crus2 and left putamen in Slow5 were higher in CP than in NP. The ALLF values of the bilateral putamen in Slow4 were increased, while the right parietal lobule in Slow4 and left upper temporal pole in Slow5 were lower in CP than in HC. The ReHo values in the CP group in the right cerebellum_crus2 was higher than that in the NP group in Slow5 (voxel p &lt; 0.05, cluster p &lt; 0.05, Gaussian Random Field theory correction). Y-balance test was correlated with cerebellum ALFF values; Tegner was moderately correlated with putamen ALFF values (p &lt; 0.05). Knee Injury and Osteoarthritis Outcome Score-sports, International Knee Documentation Committee Subjective Knee Evaluation Form and Tegner scores were correlated with the ReHo values of right cerebellum_crus2 (p &lt; 0.05).ConclusionSubcortical function transfer was performed in patients with ACLR who returned to sports postoperatively: the function of the somatosensory brain area decreased, while that of the subcortical cerebellum and basal ganglia and cerebellum ReHo increased in CP, which was correlated with clinical function. ALFF and ReHo are consistent to some extent, and sub-band studies can reveal information on different brain functions compared to the classical band.
Relationship between serum vitamin D levels and the atherogenic index of plasma: a study based on NHANES database 2011–2018
Authors: Hu Tingting , Zhang Ying , Chen Zhu , Su Jun 
Published: November 2024

ObjectiveThis study aims to investigate the relationship between serum vitamin D levels and the atherogenic index of plasma (AIP) in individuals aged 20 years and above, as well as analyze potential influencing factors.MethodsA total of 9,637 participants aged 20 years and above from the National Health and Nutrition Examination Survey (NHANES) conducted between 2011 and 2018 were included in this study. The AIP was calculated using the formula log[triglycerides (TG)/high-density lipoprotein cholesterol (HDL-C)]. Due to the skewed distribution of serum vitamin D levels in the study population, a normal transformation was performed. Weighted multivariate linear regression models were used to assess the linear relationship between the transformed serum vitamin D levels and AIP. Subgroup analysis was conducted by stratifying the data based on age, gender, and race to evaluate the stability of the relationship between serum vitamin D levels and AIP in different populations. In addition, a smooth curve fitting and generalized linear models were employed to examine the nonlinear relationship between serum vitamin D levels and AIP.ResultsAfter controlling for confounding factors, the multivariate linear regression analysis revealed a negative correlation between serum vitamin D levels and AIP [β = −0.0065, 95% CI: (−0.0106, −0.0024)]. This negative correlation was significant in male participants [β = −0.0077, 95% CI: (−0.0142, −0.0011)], Non-Hispanic Black participants [β = −0.0135, 95% CI: (−0.0211, −0.0059)], as well as participants aged 40–50 [β = −0.0124, 95% CI: (−0.0226, −0.0022)] and 60–70 [β = −0.0118, 95% CI: (−0.0214, −0.0023)]. Furthermore, a nonlinear relationship and saturation effect were observed between the transformed serum vitamin D levels and AIP, with a turning point at 8.5617 nmol/L.ConclusionOur study revealed a significant negative correlation and saturation effect between serum vitamin D levels and AIP.
Prevalence of cancer-related fatigue, associated factors and adult cancer patients’ experiences at Hawassa University Comprehensive Specialized Hospital in Ethiopia: a mixed methods study
Authors: Asefa Tseganesh , Bitew Gedamnesh , Tezera Hiwot , Tesfaye Winta 
Published: November 2024

PurposeCancer-related fatigue is a prevalent issue affecting 50–90% of cancer patients who experience fatigue at diagnosis, during therapy, and often for months or years after the completion of therapy. This study aimed to explore the prevalence of cancer-related fatigue, associated factors, and adult cancer patients’ experiences at Hawassa University Comprehensive Specialized Hospital in Ethiopia.MethodsA mixed-method study was conducted from February 25 to May 15, 2023, via cross-sectional descriptive and phenomenological approaches. The validated Amharic Brief Fatigue Inventory scale and semistructured interview guide were used. The data were processed via Epi-data version 4.4.3.1 and SPSS version 24, with logistic regression analysis. The interview records and field notes were transcribed and translated from Amharic to English and then analysed thematically.ResultsAll participants (100%) completed the study, with 77.4% reporting significant fatigue. Fatigue was strongly associated with uninsured medical expenses (P = 0.008, OR = 3.22), late-stage cancer (P = 0.000, OR = 6.11), anaemia (P = 0.009, OR = 3.71), and comorbidities (P = 0.000, OR = 7.22). From the in-depth interviews with 16 participants, two main themes emerged: financial strain (giving up basics, and inability to work) and disease progression (intensified symptoms, increased treatment side effects, and managing multiple conditions).ConclusionThis study revealed that 77.4% of cancer patients experience significant fatigue, which is linked to a lack of medical insurance, late-stage cancer, anaemia, and comorbid conditions. Financial strain limits access to care, whereas disease progression and managing multiple conditions intensify fatigue. Early intervention, financial support, and integrated care are crucial for reducing fatigue and improving quality of life. Future research should focus on multicentre and longitudinal studies to improve generalizability and track fatigue progression over time.
Development of a prognostic model incorporating a cuproptosis-related signature and CNN3 as a predictor in childhood acute myelocytic leukemia
Authors: Cao Jiafan , Xie Mengyun , Sun Kexin , Zhao Yijun , Zheng Jiayin , Wang Ying , Zheng Yucan , Liu Sixi , Yu Uet 
Published: November 2024

BackgroundChildhood acute myeloid leukemia (cAML) is the second most common pediatric blood cancer, with high heterogeneity and poor prognosis. Recent studies have highlighted cuproptosis, a newly discovered form of programmed cell death triggered by the accumulation of intracellular copper ions, as a critical mechanism influencing cancer survival and resistance. Given its emerging role in cancer biology, we investigated cuproptosis-related genes (CRGs) in cAML to explore their potential in prognostic prediction and therapeutic targeting.MethodsGene expression data from publicly available sources were analyzed to identify differentially expressed CRGs. Samples were categorized based on their expression profiles, followed by the development of a prognostic risk model using multivariable Cox regression, LASSO, and univariable analyses. The model’s performance was evaluated through Kaplan-Meier survival analysis and ROC analysis. Immune infiltration in the tumor microenvironment was assessed using ssGSEA, validated by CIBERSORT. Drug sensitivity correlations were analyzed, and functional validation experiments were conducted on THP-1 and MOLM13 cell lines to assess the role of CNN3.ResultsA set of 12 differential CRGs was used to build a robust prognostic risk model, with high accuracy in predicting patient outcomes (P &lt; 0.001). Significant differences in immune cell composition were identified between risk groups, particularly in T cells, B cells, monocytes, and dendritic cells. Drug sensitivity analysis revealed altered IC50 values for drugs like 5-fluorouracil and bortezomib. Knockdown of CNN3 in leukemia cell lines led to reduced cell proliferation.ConclusionOur CRGs-based prognostic model shows potential for guiding personalized treatment strategies in cAML. The differences in immune cell infiltration between risk groups suggest that immune modulation is key in cAML progression. CNN3 and LGR4 were identified as modulators of cAML progression, making them potential therapeutic targets. Future studies with larger cohorts are essential to validate these findings and further explore CRGs-targeted therapies.