bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical efficacy of conbercept combined with micropulse laser in patients with polypoidal choroidal vasculopathy
Authors: Liu Qingyan , Lu Yao , Sun Shuo , Shao Xiao , Yin Xin , Ma Shuying , Xue Min 
Published: October 2024

Abstract

Objective
To evaluate the clinical efficacy of conbercept combined with micropulse laser(MPL) in treating polypoidal choroidal vasculopathy (PCV).
Methods
In this prospective, randomized controlled trial conducted from February 2023 to April 2024, 52 patients (52eyes) with PCV at ### hospital were enrolled. Participants were randomized into a conbercept monotherapy group (27cases, 27eyes) and a combination treatment group (conbercept plus micropulse laser(MPL); 25cases, 25eyes). After an initial series of three intravitreal injections, all patients followed the Treat & Extend (T&E) protocol. Aqueous humor samples were collected before each of the first three injections, and cytokines levels were measured after the sample collection. The combined treatment group received a 577nm MPL application two weeks following the first injection. The best corrected visual acuity (BCVA) 、central macular thickness (CMT) and total macular volume (TMV) of each group were assessed monthly for three months.
Results
From the first to the third month post-injection, both treatment groups showed improvements in BCVA and reductions in CMT and TMV, with the combined treatment group demonstrating significantly greater improvements at each monthly evaluation (P < 0.05). Additionally, levels of aqueous humor cytokines, including VEGF-A, IL-5, MCP-1, and Ang-2, were significantly reduced in both groups. Reductions in VEGF-A and MCP-1 concentrations were positively correlated with improvements in BCVA, CMT, and TMV (P < 0.01).
Conclusion
Conbercept combined with MPL therapy demonstrates superior clinical efficacy compared to intravitreal injection of Conbercept alone in patients with PCV. Furthermore, changes in VEGF-A and MCP-1 levels may serve as predictive markers for improvements in visual acuity and retinal morphology in these patients.
Bio-synthesis of Graphitic Carbon Nitride and ZnO/GCN Nanohybrid for Remarkable Environmental Application
Authors: Panchal P. , Rauwel P. , Sharma S. , Nehra S. P. , Estephan E. , Praakle K. , Rauwel E. 
Published: October 2024

Abstract

The current study reports on biosynthesisof pure graphitic carbon nitride (GCN), ZnO nanoparticles (NPs) and ZnO-doped gaphitic carbon nitridenanohybrids (ZnO/GCN-NHs) usingOcimumtenuiflorum(OT)leafextract.GCN synthesis using plant extract was never reported in previous studies. Under direct solar lightphotocatalytic performance of the synthesized GCN, NPs and NHs was tested on the degradation of methylene orange (MO) dye and compared. Among the samples, the ZnO/GCN-NHsexhibits superior photocatalytic activity, achieving up to 47.56% degradation in 150 min of MO dye. The cytotoxicity of the biosynthesized NPs and NHswas assessed against human dental pulp stem cells and these were found to be non-toxic, indicating their potential for biomedical applications. The antimicrobial properties were also assessed using well diffusion and disc diffusion tests against four bacterial strains i.e., two Gram-negative and two Gram-positive. The tests demonstrate significant antibacterial activity with a remarkable inhibition radius against Escherichia coli 17.5 ± 1mm, Pseudomonas aeruginosa 15.04 ± 1mm, Staphylococcus aureus 27.5 ± 1mm, and Streptococcus dysgalactiae 25 ± 1 mm. The enhanced photocatalytic and antimicrobial properties of the ZnO/GCN-NHs are hypothesized to be due to the increased production of reactive oxygen species (ROS) through the combination of ZnO NPs with biosynthesized GCN.
Dual Representation Learning for One-Step Clustering of Multi-View Data
Authors: Zhang Wei , Deng Zhaohong , Choi Kup-Sze , Wang Jun , Wang Shitong 
Published: October 2024

Abstract

In real-world applications, multi-view data is widely available and multi-view learning is an effective method for mining multi-view data. In recent years, multi-view clustering, as an important part of multi-view learning, has been receiving more and more attention, while how to design an effective multi-view data mining method and make it more pertinent for clustering is still a challenging mission. For this purpose, a new one-step multi-view clustering method with dual representation learning is proposed in this paper. First, based on the fact that multi-view data contain both consistent knowledge between views and unique knowledge of each view, we propose a new dual representation learning method by improving the matrix factorization to explore them and to form common and specific representations. Then, we design a novel one-step multi-view clustering framework, which unifies the dual representation learning and multi-view clustering partition into one process. In this way, a mutual self-taught mechanism is developed in this framework and leads to more promising clustering performance. Finally, we also introduce the maximum entropy and orthogonal constraint to achieve optimal clustering results. Extensive experiments on seven real world multi-view datasets demonstrate the effectiveness of the proposed method.
Exploring the Determinants of Humanistic Care Competency in Chinese Infectious Disease Nurses Based on the HLM Model: Role of Psychological Capital and Organizational Environment
Authors: Zhai Ziyan , Wang Yu , Li Qiuyun , Meng Kai , Zhang Lili 
Published: October 2024

Abstract

Background: The humanistic care competency of infectious disease nurses plays a crucial role in clinical nursing practice. However, there is a lack of research on its influencing factors in China. This study aims to explore the influencing factors of humanistic care competency among Chinese infectious disease nurses and provide a reference basis for improving their humanistic care competency.
Methods: A stratified cluster sampling was conducted in 22 hospitals of the Chinese Infectious Disease Nursing Alliance. A total of 3135 infectious disease nurses were surveyed using self-designed questionnaires. Descriptive analysis was performed to examine the current level of humanistic care competency among infectious disease nurses. Pearson correlation analysis was used to explore the relationships between humanistic care competency, organizational environment, and psychological capital. A hierarchical linear model (HLM) was employed to investigate the effects of organizational-level organizational environment and individual-level psychological capital on humanistic care competency.
Results:Significant differences in humanistic care competency scores were observed between male and female infectious disease nurses (P＜0.05). Pearson correlation analysis revealed significant positive correlations between humanistic care competency and both nurses' psychological capital and organizational environment (P＜0.05). The HLM analysis indicated that gender, participation in training, and psychological capital had positive effects on humanistic care competency, while organizational environment exerted a negative moderating effect.
Conclusions: Nurses' psychological capital and the organizational environment of their departments influence their humanistic care competency. It is recommended that managers focus on enhancing and improving the organizational environment, strengthening psychological assessments and training. By fostering a positive psychological capital among nurses, their humanistic care competency can be stimulated.
RCN1 binds KIF14 to promote the malignant development of cervical cancer
Authors: Li Yanyu , Cai Li , Zhou Jiayun , Zhang Xuping , Zheng Yunuo , Zhang Jingbo , Cao Mengying , Cheng Hui , Wang Qing , Zhang Bei 
Published: October 2024

Abstract

Purpose
The fourth most common cause of cancer-related deaths in women is cervical cancer. Though treatment of early-stage cervical cancer is often effective, middle and advanced stage cervical cancer is hard to treat and prone to recurrence. We sought to explore the mechanism underlying cervical cancer progression to identify new therapeutic approaches.
Methods
Label-free mass spectrometry (LC-MS/MS) was used to identify differentially expressed proteins in cervical cancer and normal tissues. The findings were confirmed by Western blotting, RT-qPCR, and immunohistochemistry. The function of RCN1 in tumor invasion, metastasis, and proliferation was investigated using in vitro and in vivo tests. Immunoprecipitation tandem mass spectrometry (IP-MS) was performed in RCNI knockdown cells to identify downstream pathways.
Results
RCN1 expression is elevated in patients with lymph node metastases and recurrent cervical cancer and correlates with poor prognosis. Knockdown and overexpression assays revealed that RCN1 promotes proliferation, migration and invasion of cervical cancer cells. RCN1 overexpression encourages lung metastasis in a mouse xenograft model. Furthermore, RCN1 targets KIF14, an activator of AKT, thus providing a molecular basis that could explain the malignant behavior of RCN1-expressing cervical cancer.
Conclusion
RCN1 is significantly expressed in cervical cancer, which is associated with a poor prognosis, spread and recurrence. By promoting KIF14-induced activation of the PI3K-AKT-mTOR pathway, RCN1 may facilitate the malignant development of cervical cancer.
Spatio-temporal evolution characteristics and influencing factors of traditional villages in the Qiantang River Basin based on historical geographic information
Authors: Gao Siqi , Wang Jingxuan , Liu Shupeng , Xu Xiwei , Liao Yuqi , Zhang Zijian 
Published: October 2024

Abstract

Traditional villages serve as crucial carriers for the inheritance and development of rural historical culture. Exploring the spatial distribution characteristics of traditional villages within specific geographic units across different historical periods from a historical–geographical perspective and examining their relationships with natural and cultural environments are instrumental in understanding the regional formation mechanisms of traditional villages. This has significant implications for the holistic preservation and differentiated utilization of these villages. This study focuses on 595 traditional villages in the Qiantang River Basin and constructs a historical geographical information database. By employing ArcGIS spatial analysis, geostatistical analysis, and historical literature review methods, this study investigates the spatio-temporal distribution characteristics of these traditional villages and their influencing factors. Furthermore, the Geodetector model is used to analyze the importance of various factors influencing the distribution of traditional villages. The results indicate the following: (1) The traditional villages in the Qiantang River Basin exhibit a significant spatial clustering pattern, forming two core clusters and one belt-shaped aggregation area. (2) The historical development trajectory of traditional villages in the Qiantang River Basin can be divided into six stages: origin, expansion, peak, contraction, recovery, and saturation. The distribution center gradually shifted from the upper to the middle and lower reaches of the Qiantang River, with an expanded range and strengthened directional distribution, reflecting a north‒south spatial development sequence. (3) The spatio-temporal pattern of traditional villages in the Qiantang River Basin has been shaped by the combined effects of natural and cultural factors, with significant variation in the influence of these factors across different historical periods. The formation of traditional villages is more strongly correlated with natural factors such as elevation and water systems. This study clarifies the relationship between the evolution of traditional villages and the historical–geographical context of the Qiantang River Basin, expanding perspectives on the spatial characteristics of traditional villages and providing valuable theoretical insights into their preservation, inheritance, and development.
Title: Total thyroidectomy performed under general anesthesia with venovenous extracorporeal membrane oxygenation during a thyroid storm: A case report
Authors: Lee Hyung Gon , Bom Joon-Suk , Lee Leyeoin , Kim Joungmin 
Published: October 2024

Abstract

Background
Thyroid storm (TS) is a endocrine emergency requiring aggressive medical treatment. Occasionally, severe hemodynamic instability may occur, requiring extracorporeal membrane oxygenation (ECMO) support as a bridge to definitive surgical treatment. Generally, surgery is not recommended for patients with unstable TS, only one case was reported of total thyroidectomy performed while on venoarterial (VA)-ECMO for TS with cardiogenic shock. Here, we present the case of a 44-year-old man with refractory hypoxemia caused by pulmonary thromboembolism who underwent emergent total thyroidectomy under general anesthesia with ongoing support from venovenous (VV)-ECMO.
Case presentation:
A 44-year-old man presented to our hospital complaining of palpitations. The patient had recently experienced coronavirus disease of 2019 (COVID-19) infection, which may have aggravated the undiagnosed hyperthyroidism and resulted in thyroid storm and cardiogenic shock. Heart failure improved with immediate medical treatment and VA-ECMO. Despite continuous medical treatment, uncontrolled tachycardia persisted and pulmonary thromboembolism suddenly occurred. To improve hypoxia, VV-ECMO was performed, and medical treatment was continued with no effect. Despite the associated risks, we proceeded with thyroidectomy under general anesthesia with VV-ECMO support. On postoperative day 18, the patient was discharged without any complications.
Conclusions
This is the first case report of a total thyroidectomy performed while on VV-ECMO for TS with pulmonary thromboembolism. Although VV-ECMO may be more vulnerable to hemodynamic instability than VA-ECMO, this case shows that thyroidectomy can be performed under appropriate anesthesia management.
Dynamic transcriptome landscape of oat grain development
Authors: Wang Ting , Han Bing 
Published: October 2024

Abstract

Background Oats are widely consumed throughout the world because of their nutritive value, with their yield and quality being associated with the developmental process of grain development. However, the underlying molecular mechanisms of the transcriptional dynamics of this process have not yet been fully elucidated. In this study, RNA-seq was performed to investigate the transcriptional dynamics and identify the key genes involved in the development of the oat grain at four different developmental stages.
Results A total of 33,197 differentially expressed genes (DEGs), including 1,308 differentially expressed transcription factors (TFs) wereidentified. The main concern of this study was to include those genes associated with hormone signaling, and the sucrose and starch metabolism pathways.
Conclusions The results of this study provide valuable insights into the genetic resources affecting the molecular mechanism underlying the development of the oat grain, as well as establishing a strong theoretical foundation for its improvement.
Comprehensive orthodontic management of skeletal Class II division 2 deep overbite malocclusion with severe alveolar bone defect: A case report with two-year follow-up
Authors: Lin Yan , Xu Minrui , Xiao Yingzi , Zhong Quan , Xu Linyu 
Published: October 2024

Abstract

Background
Deep overbite is a vertical developmental anomaly of the upper and lower jaws and/or dental arches, often accompanied by sagittal and horizontal abnormalities in three-dimensional space. It can lead to restricted mandibular movement, discomfort in temporomandibular joint and masticatory muscles, and, in severe case, damage to hard tissue of the teeth and periodontal structures.
Case presentation
This case report details the orthodontic treatment of a 27-year-old female with a severe anterior alveolar bone defect, Class II skeletal malocclusion, severe crowding, a pronounced anterior deep overbite, and posterior scissor bite. The treatment aims to alleviate the deep overbite through lingual root movement and intrusion the upper anterior teeth, combined with crown reduction, while carefully managing the intrusion and labial tipping of the lower anterior teeth. Strategic asymmetric tooth extractions and a comprehensive sequential orthodontic approach such as auxiliary intrusive arch, power arms, segmental arch-wires, and mini-screws were employed. After 29 months treatment, both deep overbite and arch width discrepancies were corrected, resulting in significantly improved facial aesthetics, harmonious tooth alignment, a balanced occlusal relationship, and enhanced stability of upper and lower anterior teeth. The treatment outcomes remained stable during a two-year follow-up.
Conclusions
This case demonstrates that for adult with severe deep overbite, skeletal class II division 2 malocclusion, and poor periodontal condition, a carefully tailored treatment plan with sequential and comprehensive orthodontic management— incorporating the use of auxiliary intrusive arches, mini-screws, power arms, segmental arch-wires, and crown reduction—can successfully achieve a camouflage strategy with stable and lasting results.
Kurarinol A from Sophora flavescens as potential anti-liver fibrosis agent that inhibits the activation of LX-2 cells by regulating TGF-β/Smads signaling pathway
Authors: Chen Xing-Jun , He Ming-Hui , Zhang Xu , Liao Shang-Gao , Zeng Zhu , Li Zhu , Tu Bo , Long Qing-De , Lin Yan 
Published: October 2024

Abstract

Objective This work aims to discover bioactivity against liver fibrosis for the lavandulyl flavonoids from one botanical source of S. flavescens. Further use of transcriptomics technology to explore the molecular mechanism of anti-hepatic fibrosis.
Methods In this study, a model of LX-2 cells activation induced by transforming growth factor-β1 (TGF-β1) was established. A total of 35 free phenolics were isolated from S. flavescens to form a small compound library. These compounds on the proliferation of LX-2 cells were screened using MTS method. Furthermore, cell scratch, transcriptomics technology, real-time quantitative polymerase chain reactions (RT-qPCR) and Western blotting were used to evaluate the inhibitory effect of lavandulyl flavonoids on the proliferation and activation of LX-2 cells, and to explore the mechanism of lavandulyl flavonoids in improving liver fibrosis.
Results The results showed that a total of 11 compounds had a significant inhibitory effect on the proliferation of LX-2 cells and their IC50 was between 4-40 μM by MTS assay. Among them, 8 compounds were reported for the first time. Particularly, kurarinol A (1, IC50 12.65 μM) showed noticeable inhibitory activities. Furthermore, The results of cell scratch test showed that KA inhibited the migration of LX-2 cells. The migration process was carried out in a dose-dependent manner at 24 and 48 hours. Then, KA remarkably inhibit the mRNA and protein levels of liver fibrosis markers (α-SMA, fibronectin and collagen I), and could effectively inhibit the development of liver fibrosis. Additionally, transcriptome analysis revealed that there were 106 differentially expressed genes (DEGs) with remarkably expression differences by the treatment of KA were identified in the LX-2 cells. The mechanism studies elucidated that KA exerted protective activities involved in modulating the TGF-β/Smads signaling pathway. Among them, KA inhibited the gene and protein of TGF-β1, Smad2, Smad3, and Smad4 levels, respectively.
Conclusion KA can improve liver fibrosis. The mechanism of its anti-hepatic fibrosis was achieved by regulating the TGF-β/Smads signaling pathway. KA could be an effective anti-fibrosis agent.
Pharmacokinetics, Pharmacodynamics and Local Tolerance at Injection Site of Penicillin and Gentamicin Administered by Intravenous Regional Limb Perfusion in Standing Horses: comparison between weight bearing and flexed limbs
Authors: Guillot Marguerite , Mespoulhes-Rivière Céline , Bousquet-Melou Alain , Lacroix Marlène Z. , Roques Béatrice B. , Lallemand Elodie A. 
Published: October 2024

Abstract

Background:
Intravenous regional limb perfusion (IVRLP) of antibiotics is a therapeutic method used to treat distal limb infections in horses. This study objectives were to determine pharmacokinetic parameters and tolerance of sodium benzylpenicillin (NaBP) administered via IVRLP; to compare the feasibility, tolerance and efficacy of performing IVRLP on a flexed limb versus a weightbearing limb and finally to predict efficacy of IVRLP with gentamicin or NaBP. A prospective crossover study was performed with six horses included in the study. Each horse underwent four phases of antibiotic infusion (gentamicin 2.2mg/kg vs. NaBP 4.12mg/kg of BP) and limb position (weightbearing vs. flexed). Each antibiotic administration was followed by several synovial and blood samples to assess antibiotic concentrations. Inflammation and secondary sensibility were assessed at the injection site and at the sampled joint. A PK/PD analysis was conducted to predict the efficacy of IVRLP by estimating values of the AUC/MIC index for different minimum inhibitory concentrations (MIC).
Results:
High synovial concentrations of NaBP and gentamicin were obtained, with high variability between horses. NaBP IVRLP was well tolerated but cephalic vein inflammation score was significantly higher following NaBP infusion compared with gentamicin. For all horses and both antibiotics, synovial antibiotics exposures were significantly higher in weightbearing limbs than in flexed limbs. We found significantly higher cephalic vein inflammation scores in flexed limbs compared to weightbearing limbs. Regarding efficacy, daily dose of 2.2mg/kg of gentamicin was adequate to treat synovial infections involving E. coli or S. aureus, even when considering resistant strains. For NaBP, a daily or every-other-day dose of 4.12mg/kg of BP was adequate to treat S. equi and sensitive S. aureus synovial infections.
Conclusions:
These data suggest that daily IVRLP of NaBP (4.12mg/kg of BP) could be an effective method for treatment of infections of the distal limb for susceptible bacteria with MIC ≤ 2µg/mL, with discrete local inflammation at the injection site; whereas IVRLP with 2.2mg/kg of gentamicin could be effective to treat infections with susceptible bacteria with MIC ≤ 16µg/mL. Performing IVRLP on a weightbearing limb led to increased antibiotics exposure of the fetlock joint and to a better tolerance than the flexed limb method.
Evaluation of sea cucumber protein paste for mice’s skin wound healing and its potential anti-inflammatory mechanism
Authors: Wang Xiaoyan , Sun Jinghe , Liu Ke , Li Shuang , Zhao Jun , Yang Jingfeng 
Published: October 2024

Abstract

Natural substances with anti-inflammatory activity have always been the priority for human injuries. This study aims to investigate the beneficial effects and mechanism of sea cucumber protein (SCP) on wound healing, through a BALB/c mice model and LPS-induced RAW 264.7 cells. To find out how SCP paste works, we identified the mice's serum cytokines and tissue section. The alteration of the NF-κB pathway during the anti-inflammatory effect of SCP was also explored. The results showed that the wound healing rate in the SCP(H) group exceeded 90%, whereas it was 72.91% and 64.10% in the Control and NC groups on day 14. New blood vessels and fibroblasts were generated in the wounds. Collagen expression increased by 13.89% and 15.12% respectively in the SCP(L) and SCP(H) groups compared with the Control group on day 14. Furthermore, SCP decreased the levels of pro-inflammatory factors (TNF-α, IL-1β, IL-6) in mice’s serum while up-regulating the level of anti-inflammatory factor (IL-10) during the healing process. Furthermore, SCP suppressed the NF-κB pathway by decreasing protein levels of phosphorylated p65 and IKKα, and increasing protein levels of IκBα.