bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical significance of anti-DFS70 antibody in antinuclear antibody-positive samples with a dense fine speckled pattern
Authors: Koo Sun Hoe , Kim Jimyung , Kim Seon Young , Kwon Gye Cheol 
Published: May 2019

Abstract

Background
When the dense fine speckled (DFS) pattern-antinuclear antibodies (ANA) are detected in the indirect immunofluorescence (IIF) assay, the presence of anti-dense fine speckles 70 (DFS70) antibodies has been suggested to facilitate the exclusion of ANA-associated rheumatic diseases (AARD). We evaluated the potential use of anti-DFS70 antibodies for verifying AARD in patients with a positive ANA result of the DFS pattern.

Methods
A total of 5509 patients who were requested ANA testing were included. The DFS pattern was confirmed using two IIF assays. Semiquantitative DFS70 ELISA (Euroimmun, Germany) was examined in samples with the DFS pattern.

Results
Among 639 ANA-positive patients, 19.6% displayed the DFS pattern. And 17.6% of patients with the DFS pattern were diagnosed with AARD. The low titer of 1:80 was more prevalent in the non-AARD group than in the AARD group (64.1% vs. 4.5%, p < 0.0001). Anti-DFS70 antibodies were positive in 60.0% of patients with the DFS pattern. The frequency of anti-DFS70 positivity was higher in the non-rheumatic disease (NRD) group (74.2%) than in the other rheumatic disease group (43.2%, p = 0.003) and the AARD group (45.5%, p = 0.019).

Conclusions
The DFS pattern is present in both AARD and non-AARD cases. In the DFS pattern, a low titer of 1:80 and isolated anti-DFS70 antibodies without AARD-associated antibodies represent a low likelihood of AARD. The presence of anti-DFS70 antibodies cannot exclude AARD and should be analyzed in combination with AARD-associated antibodies in the diagnostic algorithm.
The crystal structure of tetrakis(imidazole)-copper(I) hexafluorophosphate, C12H16CuF6PN8
Authors: Zhong Wen-Wu , Tang Qian , Liu Ying-Jie , Shi Lei , Feng Yuan-Jiao , Tan Tao 
Published: May 2019

Abstract
C12H16CuF6PN8, monoclinic, C2/c (no. 15), a = 13.102(2) Å, b = 9.7902(18) Å, c = 14.727(3) Å, β = 102.336(6)°, V = 1845.3(6) Å3, Z = 4, Rgt
(F) = 0.0291, wR
ref(F
2) = 0.0733, T = 150(2) K.
Progress of using biochar as a catalyst in thermal conversion of biomass
Authors: Gholizadeh Mortaza , Hu Xun , Liu Qing 
Published: May 2019

Abstract
Biochar is a solid residual produced from the thermochemical conversion of lignocellulosic biomass via pyrolysis or gasification. It is abundantly available and has a unique structure as well as multiple functionalities. This makes biochar a potential candidate for use as a catalyst or support in catalytic reactions relating to biomass conversion such as catalytic pyrolysis, gasification, esterification of bio-oil, tar reforming, hydrothermal treatments and upgrading of bio-oil. Although numerous studies have been conducted on the potential use of biochar in various catalysis reactions, information on the overall overview and evaluation of the feasibilities of its use, especially in biomass-related conversions, is still limited. This study reviews the state-of-the-art for the production of biochar catalyst as well as its application as a catalyst or support for catalysts in producing biofuel or syngas from biomass. Special attention is given to the reaction pathway of reactants over the surface of biochar and the potential application of biochar in commercial applications. The prospects and challenges for the application of biochar as catalysts for the thermal conversion of biomass are also proposed.
Effect of Dust Ion Collision on Dust Ion Acoustic Solitary Waves for Nonextensive Plasmas in the Framework of Damped Korteweg–de Vries–Burgers Equation
Authors: Paul Niranjan , Mondal Kajal Kumar , Chatterjee Prasanta 
Published: May 2019

Abstract
Analytical solitary wave solution of the dust ion acoustic waves (DIAWs) is studied in the framework of the damped Korteweg–de Vries–Burgers (DKdVB) equation in an unmagnetised collisional dusty plasma consisting of negatively charged dust grain, positively charged ions, q-nonextensive electrons, and neutral particles. Using Reductive Perturbation Technique, the DKdVB equation is obtained for DIAWs. The effects of different physical parameters such as dust ion collision frequency parameter (

ν

i
d
0

\({\nu_{id0}}\)

), viscosity coefficient (η
10), the entropic index (q), the speed of the travelling wave (M
0), and the ratio between the unperturbed densities of the electrons and ions (μ) on the analytical solution of DIAWs are observed. The results of the present article may have applications in laboratory and space plasmas.
Frost Heave and Thawing Settlement of Frozen Soils around Concrete Piles: A Laboratory Model Test
Authors: Tang Liyun , Wang Xiaogang , Jin Long , Deng Lijun , Wu Di 
Published: May 2019

Abstract
The frost heave and thawing settlement of soils around piles could cause a series of problems, such as freezing of the pile and reduced bearing capacity of the pile. Based on the soil profiles recorded at a permafrost region in Western China, laboratory tests were performed to investigate the frost heave and thawing settlement of frozen soils around piles. The ambient temperature was adjusted in such way that the thawing and freezing of soils occurred from top to bottom, respectively. As the thawing and freezing reached different depths, the temperature, unfrozen water content, and displacements of soils around eight piles were recorded. The results showed that when the thawing was completed, the unfrozen water content of soil located at a distance equal to the pile’s diameter measured from its face was larger than that of the soil located at a distance equal to triple the pile diameters. At the end of freezing, the unfrozen water content of the soil located at a distance equal to the pile’s diameter (from the pile’s face) was smaller than that of the soil located farther (triple pile diameters). It was also shown that for the soil region between 1 and 2 times the pile diameters from its face, the amount of thawing settlement and frost heave increases, while the magnitude of the soil thawing settlement and frost heave decreases from 2 to 3 times the pile diameters. The laboratory results also showed that the difference in the frost heave and thawing settlement is related to the difference of the performance of heat transfer between the pile and soil during the freezing process. Therefore, a transverse temperature gradient exists in the pile-soil system, causing the unfrozen water to migrate toward the pile–soil interface and freeze.
Cerebellar Neurodegeneration and Neuronal Circuit Remodeling in Golgi pH Regulator-Deficient Mice
Authors: Sou Yu-shin , Kakuta Soichiro , Kamikubo Yuji , Niisato Kazue , Sakurai Takashi , Parajuli Laxmi Kumar , Tanida Isei , Saito Hiromitsu , Suzuki Noboru , Sakimura Kenji , Maeda Yusuke , Kinoshita Taroh , Uchiyama Yasuo , Koike Masato 
Published: May 2019

AbstractThe Golgi apparatus plays an indispensable role in posttranslational modification and transport of proteins to their target destinations. Although it is well established that the Golgi apparatus requires an acidic luminal pH for optimal activity, morphological and functional abnormalities at the neuronal circuit level because of perturbations in Golgi pH are not fully understood. In addition, morphological alteration of the Golgi apparatus is associated with several neurodegenerative diseases, including Parkinson’s disease, Alzheimer’s disease, and amyotrophic lateral sclerosis. Here, we used anatomical and electrophysiological approaches to characterize morphological and functional abnormalities of neuronal circuits in Golgi pH regulator (GPHR) conditional knock-out mice. Purkinje cells (PCs) from the mutant mice exhibited vesiculation and fragmentation of the Golgi apparatus, followed by axonal degeneration and progressive cell loss. Morphological analysis provided evidence for the disruption of basket cell (BC) terminals around PC soma, and electrophysiological recordings showed selective loss of large amplitude responses, suggesting BC terminal disassembly. In addition, the innervation of mutant PCs was altered such that climbing fiber (CF) terminals abnormally synapsed on the somatic spines of mutant PCs in the mature cerebellum. The combined results describe an essential role for luminal acidification of the Golgi apparatus in maintaining proper neuronal morphology and neuronal circuitry.
Hybrid Assembly of the Genome of the Entomopathogenic Nematode Steinernema carpocapsae Identifies the X-Chromosome
Authors: Serra Lorrayne , Macchietto Marissa , Macias-Muñoz Aide , McGill Cassandra Joan , Rodriguez Isaryhia Maya , Rodriguez Bryan , Murad Rabi , Mortazavi Ali 
Published: May 2019

Abstract
Entomopathogenic nematodes from the genus Steinernema are lethal insect parasites that quickly kill their insect hosts with the help of their symbiotic bacteria. Steinernema carpocapsae is one of the most studied entomopathogens due to its broad lethality to diverse insect species and its effective commercial use as a biological control agent for insect pests, as well as a genetic model for studying parasitism, pathogenesis, and symbiosis. In this study, we used long-reads from the Pacific Biosciences platform and BioNano Genomics Irys system to assemble the most complete genome of the S. carpocapsae ALL strain to date, comprising 84.5 Mb in 16 scaffolds, with an N50 of 7.36 Mb. The largest scaffold, with 20.9 Mb, was identified as chromosome X based on sex-specific genome sequencing. The high level of contiguity allowed us to characterize gene density, repeat content, and GC content. RNA-seq data from 17 developmental stages, spanning from embryo to adult, were used to predict 30,957 gene models. Using this improved genome, we performed a macrosyntenic analysis to Caenorhabditis elegans and Pristionchus pacificus and found S. carpocapsae’s chromosome X to be primarily orthologous to C. elegans’ and P. pacificus’ chromosome II and IV. We also investigated the expansion of protein families and gene expression differences between adult male and female stage nematodes. This new genome and more accurate set of annotations provide a foundation for additional comparative genomic and gene expression studies within the Steinernema clade and across the Nematoda phylum.
Differentially and Co-expressed Genes in Embryo, Germ-Line and Somatic Tissues ofTribolium castaneum
Authors: Khan Sher Afzal , Eggleston Heather , Myles Kevin M , Adelman Zach N 
Published: May 2019

AbstractTranscriptomic studies of Tribolium castaneum have led to significant advances in our understanding of co-regulation and differential expression of genes in development. However, previously used microarray approaches have covered only a subset of known genes. The aim of this study was to investigate gene expression patterns of beetle embryo, germ-line and somatic tissues. We identified 12,302 expressed genes and determined differentially expressed up and down-regulated genes among all samples. For example, 1624 and 3639 genes were differentially increased in expression greater than or equal to twofold change (FDR &lt; 0.01) in testis vs. ovary (virgin female) and ovary vs. embryo (0-5 hr), respectively. Of these, many developmental, somatic and germ-line differentially expressed genes were identified. Furthermore, many maternally deposited transcripts were identified, whose expression either decreased rapidly or persisted during embryogenesis. Genes with the largest change in expression were predominantly decreased during early embryogenesis as compared to ovary or were increased in testis compared to embryo. We also identify zygotic genes induced after fertilization. The genome wide variation in transcript regulation in maternal and zygotic genes could provide additional information on how the anterior posterior axis formation is established in Tribolium embryos as compared to Drosophila. Together, our data will facilitate studies of comparative developmental biology as well as help identify candidate genes for identifying cis-elements to drive transgenic constructs.
P wave dispersion in patients with erectile dysfunction
Authors: Öncel Can Ramazan , Akkoç Ali 
Published: May 2019

Background and aimsP wave dispersion (PWD) has been reported to be a non-invasive electrocardiographic predictor for atrial fibrillation. The aim of this study is to evaluate PWD between men with erectile dysfunction (ED) and healthy controls in order to investigate whether PWD was prolonged in patients with ED and related to severity of the disease.MethodsThis study included a total of 72 men (42 patients with ED and 30 healthy controls). Demographic data and clinical features were recorded on admission. An electrocardiographic evaluation was obtained to measure PWD values for both patients and controls.ResultsMaximum P wave duration was 108.5 ± 4.7 and 108.3 ± 4.3 in ED group and control group, respectively (p = 0.748). Minumum P wave duration was significantly higher in the control group than in the ED group. PWD was 48.1 ± 5.9 in the ED group. As a result, PWD was prolonged in patients with ED (48.1 ± 5.9 vs. 38.0 ± 3.9,p < 0.05). A significant negative correlation was observed between IIEF score and PWD values (p < 0.05,r = −0.662).ConclusionsPatients with ED exhibited prolonged PWD values compared with normal controls. In addition, PWD was found to be associated with severity of the disease.
A Coupled Operational Semantics for Goals and Commitments
Authors: Telang Pankaj R. , Singh Munindar P. , Yorke-Smith Neil 
Published: May 2019

Commitments capture how an agent relates to another agent, whereas goals describe states of the world that an agent is motivated to bring about. Commitments are elements of the social state of a set of agents whereas goals are elements of the private states of individual agents. It makes intuitive sense that goals and commitments are understood as being complementary to each other. More importantly, an agent's goals and commitments ought to be coherent, in the sense that an agent's goals would lead it to adopt or modify relevant commitments and an agent's commitments would lead it to adopt or modify relevant goals. However, despite the intuitive naturalness of the above connections, they have not been adequately studied in a formal framework. This article provides a combined operational semantics for goals and commitments by relating their respective life cycles as a basis for how these concepts (1) cohere for an individual agent and (2) engender cooperation among agents. Our semantics yields important desirable properties of convergence of the configurations of cooperating agents, thereby delineating some theoretically well-founded yet practical modes of cooperation in a multiagent system.
Tecnologia versus Desempenho Educacional: um Estudo Sobre a Performance das Escolas
Authors: Nogueira Lauro , Lima José Vitor Cosme , Vieria Bianca Alencar 
Published: May 2019

O uso de tecnologias no contexto escolar tem se tornado algo essencial nos últimos anos. Nesse ensejo, professores, alunos e demais profissionais envolvidos fazem uso frequente das mais diversas ferramentas tecnológicas em praticamente todas as atividades. Todavia, ainda não há consenso sobre a forma mais eficiente desses recursos no ambiente escolar. Diante disso, este estudo teve como principal objetivo investigar qual a influência das ferramentas tecnológicas no desempenho dos estudantes das escolas brasileiras. Para tanto, aplicou-se o método de inferências em distribuições contrafatuais desenvolvida por Chernozhukov, Fernandez-Val e Melly (2013), sobre os dados da Provinha Brasil 2013. Os principais resultados indicam que o instrumental tecnológico tem grande importância nos resultados educacionais. Porém, há diferenças substanciais conforme região e pontos da distribuição. Especificamente na Região Nordeste, a contribuição do arcabouço tecnológico cai pela metade. Por exemplo, o efeito do tratamento médio do acesso à internet de banda larga para as escolas de todo o país é de 3,4% contra 1,7% para escolas nordestinas na mesma faixa etária de resultado educacional observado. No tocante à decomposição do efeito do tratamento, os resultados informam que as características observáveis são cruciais nos resultados educacionais. Além disso, verifica-se que os efeitos dos fatores não observáveis são bastante incipientes. Palavras-chave: Tecnologia. Desempenho das Escolas. IDC. AbstractThe use of technologies in the school context has become essential in recent years. In this context, teachers, students and other professionals involved make frequent use of the most diverse technological tools in practically all activities. However, there is still no consensus on the most efficient form of these resources in the school environment. Therefore, this study had as main objective to investigate the influence of the technological tools on the students’ performance of the Brazilian schools. In order to do so, Inference Method was applied in counterfactual distributions developed by Chernozhukov, Fernandez-Val and Melly (2013), on the data of Provinha Brasil 2013. The main results indicate the technological instruments and great importance in the educational results. However, there are substantial regional nouns and distribution points. Specifically in the Northeast Region, the contribution of the technological framework is halved. For example, the average treatment effect of broadband Internet access such as schools across the country of 3.4% versus 1.7% for northeastern schools in the same age range of observed educational outcome. Regarding the decomposition of the treatment effect, the results report that the observable characteristics are crucial in educational outcomes. In addition, the effects of unobservable factors are quite insipid. Keywords: Technology. Performance of schools. IDC.
El profesorado como proyecto profesional. Prácticas y perspectivas de los actores en el inicio
Authors: Mercado Roxana , Saporiti Paola , Coronda Andrea 
Published: May 2019

In this study we investigate how a group of students started their careers as primary school teachers in 2010, by exploring different topics related to the choice of career from a position of uncertainty. For many of the students, teaching was an alternative, as they had already had experience of studying, and several knew about the transmission of knowledge, through their experience of teaching Music, Catequesis or Student Support. This was analysed from a theoretical point of view based on the notion of “working with the other” to support the choice of teaching leading to the idea of the transmission of values and religious service. However, we also questioned the idea of vocation, which we analysed from the perspective of social trajectories and habitus, in order to understand that this was a process of social construction which takes place in the family. Many of the young people consulted had family members who were teachers and they had asked their advice when they were taking the decision about entering the profession. We also discussed the question of gender in relation to teaching as a professional option. We noted a strong association between teaching and the mother figure, particularly with respect to the care and control of young children. In this study we analyse different aspects of the process of entering the teaching profession, documenting and highlighting the views and opinions of a group of student teachers as social actors, in order to understand that these processes and issues, although occurring in the context of a teacher training institution, are constructions which originate in the daily life of social and family backgrounds.
Ensayos y narrativas para pensar lo nuestro americano
Authors: Alvarado Mariana 
Published: May 2019

The "proposals for the incorporation of Latin American thought in education" generated within the framework of the project Diversity and Integration in Our America have been developed as dialogical practices of thought and writing. This article illustrates the context of the production, circulation and transfer of pedagogical mediation aimed at bringing Latin American thought to school contexts. In addition, it is concerned with the bases and actions which facilitate this inclusion as part of the contents of the curriculum.
Operative Treatment of Neer Type-II Distal Clavicular Fractures
Authors: Hohmann Erik , Tetsworth Kevin , Glatt Vaida 
Published: May 2019

» Deforming forces make it difficult to reduce and maintain reduction of Neer type-II distal clavicular fractures.» Contemporary surgical techniques are associated with high rates of union and good and excellent outcomes.» Plate fixation of distal clavicular fractures does not address horizontal and vertical instability.» Hook-plate fixation has the highest complication rate.» Augmentation of the coracoclavicular ligament is recommended to neutralize the opposing forces.
Identification of SNP Targeted Pathways From Genome-wide Association Study (GWAS) Data
Authors: Bakir-Gungor Burcu , Sezerman Osman Ugur 
Published: May 2019

Abstract

The identification of the variants that explain familial risk of a specific disease is important since it enables the development of genetic risk prediction tests, diagnosis tools and therapeutical applications. One possible reason of multifactorial diseases is the alterations in the activity of biological pathways, where a series of mutations occur in distinct genes. While each of these variations extends slightly the likelihood of having the disease, they work together to give birth to the perturbations in normal biological processes. We provide a protocol \(termed PANOGA, Pathway and Network Oriented GWAS \(Genome-wide association study) Analysis) to devise functionally important pathways through the identification of genes within these pathways, where these genes are targeted by single nucleotide polymorphisms \(SNPs) obtained from the GWAS analysis. Additionally, PANOGA helps to identify other disease related genes, not targeted by the SNPs, which are also located within these affected pathways. The program accepts tab delimited or excel file containing SNP rsIDs vs. genotypic p-values and is available at: http://akademik.bahcesehir.edu.tr/~bbgungor/panoga_protocol.zip
Multiple Iteractive Labeling by Antibody Neodeposition (MILAN)
Authors: Cattoretti Giorgio , Bosisio Francesca Maria , Marcelis Lukas , Bolognesi Maddalena Maria 
Published: May 2019

Abstract

Multiplexing, labeling for multiple immunostains the very same cell or tissue section in situ, is of considerable interest. The major obstacles to the diffusion of this technique are high costs in custom antibodies and instruments, low throughput, scarcity of specialized skills or facilities.

We have validated and detail here a method based on common primary and secondary antibodies, diffusely available fluorescent image scanners and routinely processed tissue sections \(FFPE). It entails rounds of four-color indirect immunofluorescence, image acquisition and removal \(stripping) of the antibodies, before another stain is applied. The images are digitally registered and the autofluorescence is subtracted. Removal of antibodies is accomplished by disulphide cleavage. In excess of 50 different antibody stains can be applied to one single section from routinely fixed and embedded tissue. This method requires a modest investment in hardware and materials and uses freeware image analysis software.