bluuebunny/crossref_metadata_2025_split huggingface dataset:
MiR‐409‐3p and MiR‐1896 co‐operatively participate in IL‐17‐induced inflammatory cytokine production in astrocytes and pathogenesis of EAE mice via targeting SOCS3/STAT3 signaling
Authors: Liu Xiaomei , Zhou Feng , Yang Ying , Wang Weixiao , Niu Liping , Zuo Dongjiao , Li Xiangyang , Hua Hui , Zhang Bo , Kou Yanbo , Guo Jingjing , Kong Fanyun , Pan Wei , Gao Dianshuai , Meves Jessica M. , Sun Hong , Xue Min , Zhang Qingxiu , Wang Yingwei , Tang Renxian 
Published: October 2018

AbstractTh17 cells and interleukin‐17 (IL‐17) have been found to play an important role in the pathology of multiple sclerosis (MS) and its animal model, experimental autoimmune encephalomyelitis (EAE). Response to IL‐17, reactive astrocytes accompany with immune cells infiltration and axonal damage in MS/EAE. However, the role and the regulatory mechanism of IL‐17‐activated astrocytes in inflammation and in the EAE process still remain largely unknown. Here, we elucidated that miR‐409‐3p and miR‐1896, as co‐upregulated microRNAs in activated astrocytes and in EAE mice, targeted suppressor of cytokine signaling proteins 3 (SOCS3). Overexpression of miR‐409‐3p or miR‐1896 significantly reduced SOCS3 expression and increased phosphorylation of STAT3 as well as induced the inflammatory cytokines production (IL‐1β, IL‐6, IP‐10, MCP‐1, and KC), CD4+T cells migration and demyelination, in turn aggravating EAE development. Importantly, the effects of co‐overexpression of miR‐409‐3p and miR‐1896 in vitro or in vivo are strongly co‐operative. In contrast, simultaneously silenced miR‐409‐3p and miR‐1896 co‐operatively ameliorates inflammation and demyelination in the central nervous system of EAE mice. Collectively, our findings highlight that miR‐409‐3p and miR‐1896 co‐ordinately promote the production of inflammatory cytokines in reactive astrocytes through the SOCS3/STAT3 pathway and enhance reactive astrocyte‐directed chemotaxis of CD4+T cells, leading to aggravate pathogenesis in EAE mice. Co‐inhibition of miR‐409‐3p and miR‐1896 may be a therapeutic target for treating MS and neuroinflammation.
Prognostic role of baseline 18F‐FDG PET/CT parameters in MALT lymphoma
Authors: Albano Domenico , Bosio Giovanni , Camoni Luca , Farina Mirko , Re Alessandro , Tucci Alessandra , Giubbini Raffaele , Bertagna Francesco 
Published: October 2018

AbstractMucosa‐associated lymphoid tissue (MALT) lymphoma is an indolent lymphoma with good prognosis and variable fluorine‐18 fluorodeoxyglucose (18F‐FDG) avidity. Many possible prognostic factors have been investigated with controversial results, but the possible prognostic role of 18F‐FDG positron emission tomography/computed tomography (PET/CT) remains unclear. Our aim was to evaluate the prognostic impact of qualitative and semiquantitative baseline PET/CT parameters on outcome of MALT lymphoma. We retrospectively enrolled 161 patients with histologically confirmed MALT lymphoma who underwent 18F‐FDG PET/CT before any treatment. PET images were qualitatively and semiquantitatively analyzed by measuring the maximum standardized uptake value body weight (SUVbw), lean body mass (SUVlbm), body surface area (SUVbsa), metabolic tumor volume (MTV), and total lesion glycolysis (TLG). The Kaplan‐Meier method was used to estimate the progression‐free survival (PFS) and overall survival (OS) times. Cox regression models were performed to determine the relation between PET/CT features and OS and PFS. Ninety‐eight patients had positive 18F‐FDG PET/CT showing 18F‐FDG uptake (mean SUVbw, 10.1; SUVlbm, 7.2; SUVbsa, 2.7; MTV, 88.8; and TLG, 526); the remaining 63 were not 18F‐FDG avid. 18F‐FDG avidity was significantly correlated with tumor size and Ki‐67 score. Relapse/progression of disease occurred in 47 patients with an average time of 40.2 months; death occurred in 12 patients with an average of 59 months. At a median follow‐up of 62 months, median PFS and OS were 52 and 62 months, respectively. Advanced tumor stage and extragastric site were demonstrated to be independent prognostic factors for PFS, while only tumor stage for OS. Instead, PET/CT parameters were not related to survival, despite positive correlation at univariate analysis between MTV and TLG with PFS and positive PET/CT with PFS and OS. In conclusion, a 61% rate of PET avidity in biopsy‐confirmed MALT lymphoma was found, and it was correlated with tumor size and Ki‐67 score. Only tumor stage and localization were independently correlated with PFS and OS.
Understanding Practitioner‐Driven Assessment and Evaluation Efforts for Social Justice
Authors: Zerquera Desiree , Reyes Kimberly A. , Pender Jason T. , Abbady Ramy 
Published: October 2018

AbstractThis chapter presents findings from a national survey that sought to capture the ways practitioners work to integrate both assessment and social justice into their work as central concepts and practices that guide their efforts in student affairs. In so doing, these practitioners are working to reclaim assessment from the traditional, dominant framing of assessment work through neoliberal lenses and advance social justice in higher education.
Are DNA damage response kinases a target for the differentiation treatment of acute myeloid leukemia?
Authors: Pennisi Rosa , Albanesi Jacopo , Ascenzi Paolo , Nervi Clara , di Masi Alessandra 
Published: October 2018

AbstractAcute myeloid leukemia (AML) is a genetically heterogeneous malignancy characterized by the expansion of hematopoietic stem/progenitor cells (HPCs) blocked at different stages of maturation/differentiation. The poor outcome of AMLs necessitates therapeutic improvement. In AML, genes encoding for myeloid transcription factors, signaling receptors regulating cell proliferation, and epigenetic modifiers can be mutated by somatically acquired genetic mutations or altered by chromosomal translocations. These mutations modify chromatin organization at genes sites regulating HPCs proliferation, terminal differentiation, and DNA repair, contributing to the development and progression of the disease. The reversibility of the epigenetic modifications by drug treatment makes epigenetic changes attractive targets for AML therapeutic intervention. Recent findings underline increased DNA damage and abnormalities in the DNA damage response (DDR) as a critical feature of AML blasts. The DDR preserves cell integrity and must be tightly coordinated with DNA methylation and chromatin remodeling to ensure the accessibility to the DNA of transcription factors and repair enzymes. A crucial role in these events is played by the ataxia telangiectasia mutated (ATM) and ataxia telangiectasia and Rad3‐related protein (ATR) kinases, which are hyperactive in AML. Based on these findings, we hypothesize the inhibition of DNA damage kinases as an alternative or complementary strategy for the differentiation treatment of AML as it leads to a reduced ability to repair the DNA damage, and to the inhibition of specific epigenetic modifiers whose function is altered in leukemic cells. © 2018 IUBMB Life, 70(11):1057–1066, 2018
Implementation and evaluation of a pharmacy‐driven transitions of care program for patients discharged from the emergency department
Authors: Lacy Mary C. , Bryant Ginelle A. , Herring Morgan S. , Koenigsfeld Carrie F. , Lehman Nicholas P. , Smith Hayden L. 
Published: October 2018

PurposeTo evaluate a pharmacy‐driven post‐emergency department (ED) transitions of care program.MethodsPatients admitted to the ED who receive primary care at a network clinic were eligible for inclusion. Pharmacy team members contacted patients within 7 days of ED discharge to perform medication reconciliation and assess for appropriate follow‐up. Following the calling period, rates of repeat ED visits, urgent care visits, hospitalizations, and clinic visits within 30 days of ED discharge were evaluated.ResultsSeventy percent of eligible patients were successfully contacted via phone. At least one new medication was prescribed in the ED to 61% of contacted patients, 22% of which were not picked up at the time of the call. Medication reconciliation was successfully completed during 87% of the phone calls, with 64% resulting in at least one identified medication discrepancy. A total of 230 pharmacist interventions were completed. Contacted patients had an adjusted 44% (95% confidence interval [CI]: 9–65) lower risk of subsequent ED visits compared with patients unable to be reached. Four percent of patients successfully contacted were admitted to the hospital within 30 days of ED discharge compared with 8% of patients unable to be reached (adjusted relative risk [RR]: 0.39; 95% CI: 0.18–0.84).ConclusionPatients who received post‐ED transitions of care calls were less likely to be seen again in the ED or be admitted to the hospital within 30 days. Follow‐up phone calls provided by pharmacy team members may be an effective tool to improve transitions of care following ED visits.
Rapid identification of gram‐negative bacteremia and impact on antipseudomonal antibiotic consumption with antimicrobial stewardship at a community hospital system
Authors: Box Maggie J. , Lee Jennifer M. , Ortiz Courtney D. , Ortwine Kristine N. , Richardson Caitlin A. , Sullivan Eva L. , Wong Samantha S. 
Published: October 2018

BackgroundRapid identification of gram‐negative bacteremia can improve time to effective antibiotic therapy and antibiotic de‐escalation; however, there are little data that quantify the change in antibiotic utilization. This study evaluates the impact of the Verigene gram‐negative blood culture test (BC‐GN) in combination with an antimicrobial stewardship team (AST) intervention on antipseudomonal (AP) antibiotic utilization in a community hospital system among infections where AP antibiotics are not necessary.MethodsThis multicenter, pre‐post, quasi‐experimental pilot study was conducted at Scripps Health in San Diego, California. Internal antibiogram data was reviewed, and among the 4 targets of Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca, and Proteus species (that lacked resistance genes) detected by the BC‐GN test, antimicrobial sensitivities did not warrant therapy with AP antibiotics. The BC‐GN test was performed at each site where clinical pharmacists notified physicians of results and assisted with antibiotic modifications. The primary end point was AP vs non‐AP antibiotic consumption within the first 5 days of admission. This was measured by days of therapy per patient day (DOT/PD). Secondary end points included hospital and intensive care unit (ICU) length of stay (LOS) and mortality.ResultsA total of 1051 patients were included; 512 in the pre‐intervention group, and 539 in the intervention group. Compared with the pre‐intervention group, AP antibiotic consumption significantly decreased (0.4 vs 0.2 DOT/PD, P < .0001) and non‐AP antibiotic consumption significantly increased (0.6 vs 0.8 DOT/PD, P < .0001) in the intervention group. Overall median LOS was 5 days vs 5 days (P = .85) and median ICU LOS was 3 days vs 2 days (P = .12), in the pre‐intervention and intervention groups, respectively. Mortality was similar between groups (7.0% vs 5.2% in the pre‐intervention and intervention groups, P = .21).ConclusionRapid identification of gram‐negative bacteremia with AST intervention decreased AP antibiotic consumption among infections caused by pathogens where AP coverage is not needed.
Systematic evaluation of the methodological quality of clinical practice guidelines intended for pharmacists
Authors: Beckett Robert D. , Linn Dustin D. , Tellor Katie B. , Sheehan Amy H. , Montagano Kaitlin J. , Vonada Brooke 
Published: October 2018

IntroductionThe Appraisal of Guidelines, Research, and Evaluation version II (AGREE II) tool is a validated instrument designed to assess the methodological rigor and transparency of clinical practice guidelines.ObjectiveThe primary objective of this study was to determine the quality of clinical practice guidelines of which pharmacists are indexed as an intended user using AGREE II.MethodsAGREE II was used to assess for methodological rigor and transparency in a systematic evaluation. The National Guideline Clearinghouse was used to identify guidelines published from 2010 to 2016, focused on treatment, and indexed to pharmacists as potential intended users. Each guideline was reviewed according to AGREE II by 3 independent reviewers: 1 clinical specialist pharmacist, 1 drug information pharmacist, and 1 student pharmacist, with domain and item scores calculated according to the AGREE II User Manual.ResultsOf 140 identified guidelines, mean AGREE II domain scores ranged from 54.8 ± 20.1% (Domain 5, Applicability) to 86.5 ± 9.0% (Domain 4, Clarity of Presentation). Mean AGREE II item scores ranged from 38.2 ± 34.4% (Item 5, “The views and preferences of the target population have been sought”) to 91.4 ± 11.2% (Item 17, “Key recommendations are easily identifiable”). For Item 24 (“Rate the overall quality of this guideline”), the mean score was 71.5 ± 17.8% (median: 75.0%). For Item 25 (“I would recommend this guideline for use”), the most common responses were “yes, with modifications” (n= 66, 47.1%) and “yes” (n= 57, 40.7%).ConclusionGuidelines intended for pharmacists were generally high quality, with opportunity for improvement in areas related to applicability.
Effect of marital relationship on women's quality of life: Testing the mediating role of subjective well‐being
Authors: Wang Hua , Xu Congde , Fan Xiaoyan 
Published: October 2018

AbstractBecause of a large number of Chinese women and the widespread concern about women's living conditions, this study aimed to examine how subjective well‐being mediates the association between marital relationship and women's quality of life in Chinese context. The data used in this study were collected by conducting a multi‐stage cluster random sampling method of 756 women in Huai'an city of Mainland China. Data included women's basic demographics, women's quality of life and marital quality, and women's subjective well‐being. Structural equation modeling was adopted to analyze the data. The results of structural equation modeling analysis suggested a good fit for the sample. Overall findings showed that marital relationship has a significantly direct effect on women's quality of life, and subjective well‐being can partially mediate the impact of marital relationship on women's quality of life. The implications on theory and social policy were also discussed.
Evaluation of cell proliferation in cystic lesions associated with impacted third molars
Authors: Embaló Bubacar , Parize Hian Nivaldo , Rivero Elena Riet Correa 
Published: October 2018

AbstractThe purpose of this study was to evaluate the metabolism and epithelial cell proliferation of odontogenic keratocyst (OKC), dentigerous cyst (DC), and unicystic ameloblastoma (UA) by quantifying the nucleolar organizing regions (AgNORs) and Ki‐67 protein immunoexpression. Forty‐eight cases (16 OKC, 16 DC, and 16 UA) were evaluated retrospectively. The metabolism and epithelial cell proliferation was measured by the Ki‐67 positive cell percentage index and by the mean AgNOR count in each group. The Ki‐67 and AgNOR counts were significantly higher in OKC comparing to the DC and UA (p < .001). Ki‐67 positive cells were observed higher in suprabasal cell layers of OKC with uniform distribution, a few of them were predominantly observed in basal cell layer in DC and UA. The AgNOR count was significantly higher in the OKC basal cell layers and observed throughout the lining epithelium of DC and UA. Ki‐67 and AgNOR reinforced the aggressive character of OKC, presenting high metabolism and cellular proliferation compared to DC and UA, possibly due to its more aggressive clinical behavior and high recurrence rate.Research Highlights

We evidence higher metabolism and epithelial cell proliferation in OKC when compared to UA and DC, supporting its aggressive aspect and its high rate of recurrence.
OKC had intense and predominant labeling of Ki‐67 on the suprabasal layer unlike UA and DC.
Morphological evaluation of liposomal iron carriers
Authors: Battistelli Michela , Salucci Sara , Falcieri Elisabetta 
Published: October 2018

AbstractIron is one of the most important elements for human, because it plays an essential role in many metabolic processes. However, it is also recognized to be dangerous for its detrimental effect inside human cells, where, in the absence of homeostatic balance, it can induce free radicals formation. Moreover, an excessive accumulation of iron in tissues can produce iron overload, a condition incompatible with life. The use of liposomes as carriers can represent an interesting iron therapy to improve iron bioavailability and reduce its negative effects, in particular during pregnancy. In this study, a morphological analysis has been performed on commercial liposome vesicles at various drying times, both in saline solution and in distilled water. Furthermore, to highlight their possible interaction or internalization in cells, liposomes have been administered to human hemopoietic U937 cells. Ultrastructural analyses confirm that vesicle morphology and size are comparable with classical liposomal structures. Products are stable during specimen preparation and drying. Additionally, they have a good ability to penetrate into cells, interacting with cytoplasmic organelles, without inducing, at least apparently, any ultrastructural damage.
Histomorphometry of ectopic mineralization using undecalcified frozen bone sections
Authors: Fujihara Ryuji , Chiba Yoichi , Nakagawa Toshitaka , Murakami Ryuta , Matsumoto Koichi , Kawauchi Machi , Fujii Takayuki , Shimono Ryuichi , Yamamoto Tetsuji , Ueno Masaki 
Published: October 2018

AbstractTo investigate the correlation between mineral formation and enhanced expressions of some proteins using undecalcified frozen bone sections. Histological studies have revealed that some proteins, such as BMP2, BMPR1A, and Connexin 43, are expressed in and around sites of ectopic ossification. However, the relationship between the expressed proteins considered to be associated with the ossification and mineral formation in vivo is not clear. Ectonucleotide pyrophosphatase phosphodiesterase 1 (ENPP1)‐mutant spinal hyperostotic TWY mice and ICR mice as controls were euthanized after calcein labeling, and undecalcified frozen sections were obtained from the middle thoracic spine. Intervertebral disc areas were examined histologically and by measuring calcein‐labeled areas and areas showing immunoreactivity for BMP2, BMPR1A, and Connexin 43. Calcein‐labeled areas, indicating mineralization in the ectopic mineralization sites, were significantly larger in the mutant mice than in controls. The expression of Connexin 43 was elevated in the annulus fibrosus. Increases in the calcein‐labeled areas was not correlated with increases in the areas showing immunoreactivity for Connexin 43 in the annulus fibrosus. There was no statistical correlation between enhanced immunohistochemical expression and elevated calcein‐labeled areas. By applying the morphometrical analysis method using undecalcified frozen sections to ENPP1‐mutant mice, quantitative evaluation of the mineralization and proteins expressed in the surrounding area in the same animal became possible.
JAG2 signaling induces differentiation of CD14+ monocytes into Langerhans cell histiocytosis-like cells
Authors: Schwentner Raphaela , Jug Gunhild , Kauer Maximilian O , Schnöller Thomas , Waidhofer-Söllner Petra , Holter Wolfgang , Hutter Caroline 
Published: October 2018

Abstract
Langerhans cell histiocytosis (LCH) is a MAPK pathway-driven disease characterized by the accumulation of CD1a+langerin+ cells of unknown origin. We have previously reported that the Notch signaling pathway is active in LCH lesions and that the Notch ligand Jagged2 (JAG2) induces CD1a and langerin expression in monocytes in vitro. Here we show that Notch signaling induces monocytes to acquire an LCH gene signature and that Notch inhibition suppresses the LCH phenotype. In contrast, while also CD1c+ dendritic cells or IL-4-stimulated CD14+ monocytes acquire CD1a and langerin positivity in culture, their gene expression profiles and surface phenotypes are more different from primary LCH cells. We propose a model where CD14+ monocytes serve as LCH cell precursor and JAG2-mediated activation of the Notch signaling pathway initiates a differentiation of monocytes toward LCH cells in selected niches and thereby contributes to LCH pathogenesis.
This study aims to identify the cell of origin of Langerhans Cell Histiocytosis and the molecular mechanisms of its pathogenesis.
Diversity of oil yield, fatty acids, tocopherols, tocotrienols, and sterols in the seeds of 19 interspecific grapes crosses
Authors: Górnaś Paweł , Rudzińska Magdalena , Grygier Anna , Lācis Gunārs 
Published: October 2018

AbstractBACKGROUNDThe seeds of Vitis vinifera grapes have been studied extensively but knowledge about the interspecific crosses of other Vitis species (e.g. V. vinifera, V. amurensis, V. rupestris, V. riparia, and V. labruska) is very limited.RESULTSThe oil yields recovered from the grape seeds ranged between 7 and 160 g kg−1 dw. The main fatty acids were linoleic (72.5–83.1%), oleic (6.2–15.5%), and palmitic (5.4–13.2%), which together constituted 92.8–97.1% of the total detected fatty acids. The total concentration of tocopherol (T) and tocotrienol (T3) homologues was between 0.785 and 9.033 g kg−1 oil. The concentration of sterols varied significantly and ranged between 2.912 and 105.962 g kg−1 oil. The β‐sitosterol constituted 68.2–86.3% of the total content of sterols. The oil yield in grape seeds significantly correlated with the oleic acid, α‐linolenic acid, α‐T, α‐T3, γ‐T3, campesterol, Δ5‐stigmasterol, β‐sitosterol, and total Ts + T3 s and sterols.CONCLUSIONThe present study demonstrated that seed oil recovered from different interspecific Vitis crosses is a rich source of minor lipophilic bioactive compounds, especially genotypes with low oil content. They can be used to enrich plant oils that are poor in tocotrienols and/or phytosterols without changing the fatty acid composition of main oil, due to low enrichment quantities (micro‐blends). © 2018 Society of Chemical Industry
Sensory evaluation of low‐fat yoghurt produced with microbial transglutaminase and comparison with physicochemical evaluation
Authors: García‐Gómez Belén , Romero‐Rodríguez Ángeles , Vázquez‐Odériz Lourdes , Muñoz‐Ferreiro Nieves , Vázquez Manuel 
Published: October 2018

AbstractBACKGROUNDLow‐fat or non‐fat yoghurts are popular nowadays. However, their texture is not good and they show considerable syneresis with time. The aim of this research was to evaluate the effect of the application of transglutaminase (TG) to low‐fat yoghurt to determine if similar sensory properties to those of full‐fat yoghurt can be obtained. Methods of adding TG (prior or simultaneous with fermentation), dose, and TG origin were evaluated. Correlations between sensory and physicochemical parameters were assessed.RESULTSThe results showed no significant differences between TG addition methods in terms of the quantitative and qualitative sensory attributes studied. Simultaneous addition of TG was preferred to speed up the process. A dose of 1 U g−1 was selected as optimal. Few differences were detected due to the origin of the TG. They were not significant although some differences were observed in terms of density, bitterness, and syneresis compared with low and full‐fat yoghurts.CONCLUSIONThe results showed that sensory parameters cannot be associated with only one physicochemical parameter in yoghurt evaluation. Transglutaminase can be used as a substitute for stabilizers in the production of low‐fat yoghurt, maintaining good sensory properties and avoiding initial syneresis. © 2018 Society of Chemical Industry