bluuebunny/crossref_metadata_2025_split huggingface dataset:
Service Quality in Spectator Sports: A Review and Research Agenda
Authors: Biscaia Rui , Ramos Ricardo Filipe , Yoshida Masayuki , Kim Yukyoum 
Published: October 2024

ABSTRACTAlthough service quality in spectator sports has been subject to extensive research, comprehensive studies mapping the intellectual structure and foundations of service quality in spectator sports are scant. The purpose of the current study was to synthesise and analyse the literature on service quality in spectator sports, and set a new research agenda by conducting a hybrid integrated review of the articles between 1996 and 2023. A total of 149 peer‐reviewed journal articles were analyzed. First, a bibliometric analysis provided the citation structure and evolution of publications, source impact, most cited articles, most productive authors and associated impact. Then, a framework‐based review including the articles' context, theories, type of study, service quality dimensions, spectator outcomes and limitations was conducted to provide a comprehensive overview of the current state of knowledge and identify future research directions. This study contributes to the literature by uncovering the structure and intellectual base of existent research, identifying gaps and suggesting a research agenda to deepen the understanding and management of service quality in spectator sports.
Quality problems in clinical practice guidelines and guideline appraisal studies: Should we tolerate or eradicate?
Authors: Yang Guo‐Xun , Dou Shu‐Qian , Liu Xiao‐Bo , Que Ting , Tang Yong , Wang Xin , Yan Long‐Zong , Zhou Li‐Na , Jin Cheng‐Bo , Wang Yuan , Wang Qi , Wu Kong‐Jia , Liu Wen‐Jun 
Published: October 2024

AbstractBackgroundAppraisal of Guidelines for Research and Evaluation (AGREE) II instrument have been widely used by scholars around the world to assess the methodological quality of clinical practice guidelines (CPGs). We sought to identify items or domains that are commonly scored low in the assessment, and to systematically review the issues that emerged when evaluators used the AGREE II tool for guideline quality assessment.MethodsA systematic search was conducted to identify articles published in medically relevant databases from 2022 to 2023 regarding the use of the AGREE II tool for the assessment of CPGs. We extracted six quality domains and overall quality assessment data of CPGs included in the literature, and processed the data using descriptive statistical analysis, difference analysis, regression analysis, and correlation analysis. A seven‐point Likert scale was used to assess the reporting quality of the included articles.Results151 relevant publications were identified, including 2081 guidelines published between 1990 and 2022. The results of the regression analysis showed a statistically significant impact of all domains on overall guideline quality (p < 0.001; R2 = 0.777). Domain 1, 2, 3, 4, and 6 scores differed significantly over time (p < 0.001) and were increasing. The score was good for Domain 4 (median 78.00 [IQR: 62.75–89.00]; mean 74.34 [SD 18.85]) and Domain 1 (median 78.00 [IQR: 61.00–90.00]; mean 73.57 [SD 21.12]). Scores were generic for Domain 6 (median 58.33 [IQR: 25.00–83.33]; mean 53.98 [SD 34.13]), Domain 2 (median 53.00 [IQR: 33.30–72.10]; mean 53.30 [SD 24.52]) and Domain 3 (median 51.00 [IQR: 26.02–73.00]; mean 50.44 [SD 27.19]). The score was poor for Domain 5 (median 36.20 [IQR: 20.20–58.32]; mean 40.21 [SD 24.90]). In addition, the quality evaluation results of the included articles showed that 33.1% were evaluated as low and 11.9% as very low.ConclusionsAGREE II tools have facilitated the development of methodological quality for CPGs. Although the quality of CPGs has improved over time, some general low‐quality problems still exist, and solving these problems will be an effective way for developers to upgrade the quality of guidelines. In addition, addressing critical issues in the evaluation of guidelines to present high‐quality study reports would be another way to guide guideline development.
In Situ Diagnosis and Digital Cataloguing of Plant Pathogenic Fungi Through Mobile‐Based Foldscope Microscopy
Authors: Mooventhan Palanisamy , Sivalingam Palaiyur Nanjappan , Singh Harvinder Kumar , Sahu Manoj Kumar , Dhimar Yogita , Singh Uttam , Kaushal Pankaj , Ghosh Probir Kumar 
Published: October 2024

ABSTRACTAgriculture confronts multifaceted challenges across the spectrum of crop production, with pest and disease management being a prominent concern. Timely diagnosis of crop diseases is imperative for mitigating production costs and curbing the adverse environmental impacts of chemical pesticides. In the present investigation, mobile phone‐based foldscope microscopy (MBFM) was used to diagnose various field samples infected with fungal diseases of field and horticultural crops, and the same was validated with the normal microscope pictures and field symptoms. The MBFM was also used to diagnose seed‐borne microflora associated with wheat and spores of commercial formulation of bioagents and validated. The MBFM utilises both symptoms and morphological structures of pathogen for in situ field diagnosis and hence advantages over the symptom‐based mobile Apps. This study underscores the utility of foldscope microscope as a potent technique for plant pathologists and extension workers to enable real‐time and in situ identification of diseases caused by fungal pathogens in various agricultural crops.
Estimation of Metabolizable Amino Acids and Energy Requirements and Development of Mathematical Models to Predict Average Daily Gain for Beef Cattle
Authors: Wang Jiaqi , Shen Chun , Zhao Guangyong , Li Meng M. 
Published: October 2024

ABSTRACTThis study aimed to estimate metabolizable energy (ME), protein (MP) and amino acids (MAA) requirements for maintenance and growth, and to develop a mathematical model to predict ADG for beef cattle using a meta‐regression approach. A data set including 91 sources of literature with 385 treatments was assembled after data collection and screening. To estimate nutrient requirements for maintenance and growth, metabolizable nutrient intakes were regressed on metabolic body weight (MBW) and ADG using a mixed model with a random study effect. A multiple linear regression model was derived to predict ADG using MBW and nutrient intakes as independent variables. The random forest analysis was used to identify the importance of independent variables associated with ADG and compare it with the multiple linear regression model. The accuracy of the model was evaluated by Monte−Carlo cross‐evaluation. The ME and MP requirements for maintenance were 0.56 MJ/BW0.75 and 4.31 g/BW0.75. The maintenance requirements of metabolizable arginine (Arg), histidine (His), isoleucine (Ile), leucine (Leu), lysine (Lys), methionine (Met), threonine (Thr), tryptophan (Trp), phenylalanine (Phe), valine (Val) and total AA were 0.044, 0.073, 0.013, 0.13, 0.10, 0.024, 0.075, 0.026, 0.088, 0.11 and 0.76 g/BW0.75. The estimated ME and MP requirements for growth were 45.8 MJ/kg ADG and 398.7 g/kg ADG. The growth requirements of metabolizable Arg, His, Ile, Leu, Lys, Met, Thr, Trp, Phe, Val and total AA were 4.5, 6.4, 7.3, 24.0, 2.9, 2.4, 6.1, 1.7, 9.5, 9.3 and 74.5 g/kg ADG. The multiple linear regression analysis showed that ADG was positively correlated with intakes of ME, ether extract, starch and Trp (p < 0.05). The results of the Monte−Carlo cross‐evaluation showed that the multiple regression model was more accurate in predicting ADG than the random forest model. ME and MP requirements for maintenance are consistent with previous studies, but requirements for growth tend to be overestimated. Future studies are needed to evaluate MAA requirements. An accurate model was developed to predict ADG, offering a theoretical basis and guidance to improve growth performance and feed conversion efficiency for beef cattle.
A Genomic Exploration of the Possible De‐Extirpation of the Zanzibar Leopard
Authors: Sun Xin , Cavill Emily Louisa , Margaryan Ashot , Lin Jianqing , Thingaard Søren , Said Tamrini A. , Gopalakrishnan Shyam , Gilbert M. Thomas P. 
Published: October 2024

ABSTRACTThe recently extirpated Zanzibar leopard was the only known African leopard (Panthera pardus spp.) population restricted exclusively to a major island habitat. Although its demise was driven through habitat change and conflict with humans, given its role as a keystone species for the Zanzibar Archipelago, its successful potential reintroduction might offer a means for helping preserve the natural biodiversity of its former habitat. Whether this is feasible, however, would be contingent on both whether closely related source populations can be identified on mainland Africa, and whether the Zanzibar form exhibited any special adaptations that might need to be considered when choosing such a source. In light of these questions, we genomically profiled two of the six known historic specimens, to explore whether they represent a realistic candidate for de‐extirpation through reintroduction. Our analyses indicate that despite its geographical separation, the Zanzibar leopard shared a close genetic relationship with mainland East African individuals. Furthermore, although its uniqueness as an island population was emphasised by genomic signatures of high inbreeding and increased mutation load, the latter similar to the level of the critically endangered Amur leopard (P. p. orientalis), we find no evidence of functionally significant genetic diversity unique to Zanzibar. We therefore conclude that should attempts to restore leopards to Zanzibar be considered, then mainland East African leopards would provide a suitable gene pool.
Autoencoder‐based flow‐analogue probabilistic reconstruction of heat waves from pressure fields
Authors: Pérez‐Aracil Jorge , Marina Cosmin M. , Zorita Eduardo , Barriopedro David , Zaninelli Pablo , Giuliani Matteo , Castelletti Andrea , Gutiérrez Pedro A. , Salcedo‐Sanz Sancho 
Published: October 2024

AbstractThis paper presents a novel hybrid approach for the probabilistic reconstruction of meteorological fields based on the combined use of the analogue method (AM) and deep autoencoders (AEs). The AE–AM algorithm trains a deep AE in the predictor fields, which the encoder filters towards a compressed space of reduced dimensionality. The AM is then applied in this latent space to find similar situations (analogues) in the historical record, from which the target field can be reconstructed. The AE–AM is compared to the classical AM, in which flow analogues are explicitly searched in the fully resolved field of the predictor, which may contain useless information for the reconstruction. We evaluate the performance of these two approaches in reconstructing the daily maximum temperature (target) from sea‐level pressure fields (predictor) recorded during eight major European heat waves of the 1950–2010 period. We show that the proposed AE–AM approach outperforms the standard AM algorithm in reconstructing the magnitude and spatial pattern of the considered heat wave events. The improvement ranges from 7% to 22% in skill score, depending on the heat wave analyzed, demonstrating the potential added value of the hybrid method.
Shifts in plant architecture drive species‐specific responses to drought in a Sorghum recombinant inbred line population
Authors: Lehrer M. A. , Govindarajulu R. , Smith F. , Hawkins J. S. 
Published: October 2024

Abstract
Drought stress severely impedes plant growth, development, and yield. Therefore, it is critical to uncover the genetic mechanisms underlying drought resistance to ensure future food security.
To identify the genetic controls of these responses in Sorghum, an agriculturally and economically important grain crop, an interspecific recombinant inbred line (RIL) population was established by crossing a domesticated inbred line of Sorghum bicolor (TX7000) with its wild relative, Sorghum propinquum. This RIL population was evaluated under drought conditions, allowing for the identification of quantitative trait loci (QTL) that contribute to drought resistance.
We detected eight QTL in the drought population that explain a significant portion of the observed variation for four traits (height, aboveground biomass, relative water content, and leaf temperature/transpiration). The allelic effects of, and the candidate genes within, these QTL emphasize: (1) the influence of domestication on drought‐responsive phenotypes, such as height and aboveground biomass, and (2) how control of water uptake and/or loss can be driven by species‐specific plant architecture.
Our findings shed light on the interconnected roles of shoot and root responses in drought resistance as it relates to regulation of water uptake and/or loss, while the detected allelic effects demonstrate how maintenance of grain production and yield under drought is a likely result of domestication‐derived drought tolerance.
Ethylamine, beyond the synthetic precursor of theanine: CsCBF4‐CsAlaDC module promoted ethylamine synthesis to enhance osmotic tolerance in tea plants
Authors: Zhou Ziwen , Luo Xiangzong , Fu Maoyin , Li Siya , Cheng Yaohua , Li Yeyun , Zhang Xianchen 
Published: October 2024

SUMMARYThe tea plant (Camellia sinensis) is a perennial green plant, and its tender leaves are rich in secondary metabolites, such as theanine. Ethylamine (EA), a small amine, is an important prerequisite for theanine synthesis. However, beyond its involvement in theanine synthesis, the other physiological functions of EA in tea plants remain unknown. In vitro experiments indicate that EA may function as scavengers of reactive oxygen species (ROS) to protect the plant against damage caused by osmotic stress. Additionally, a significant correlation between EA levels and osmotic tolerance has been observed in different tea varieties. From the results, alanine decarboxylase (CsAlaDC)‐silenced tea leaves and overexpressed CsAlaDC Arabidopsis thaliana lines decreased and increased EA levels, respectively, and mediated ROS homeostasis, thus exhibiting a sensitive and tolerant phenotype. In addition, the transcription factor (TF) CsCBF4 was functionally identified, which can directly bind to the CsAlaDC promoter. CsCBF4‐silenced tea leaves significantly reduced the expression levels of CsAlaDC and in turn EA content, resulting in excess ROS accumulation and an osmotic‐sensitive phenotype. Taken together, these results established a new regulatory module consisting of CBF4‐CsAlaDC responsible for EA accumulation and ROS homeostasis in response to osmotic stress.
Stress physiology of Moringa oleifera under tropospheric ozone enrichment: An ecotype‐specific investigation into growth, nonstructural carbohydrates, and polyphenols
Authors: Baesso Moura Bárbara , Hoshika Yasutomo , Brunetti Cecilia , dos Santos Nascimento Luana Beatriz , Marra Elena , Paoletti Elena , Ferrini Francesco 
Published: October 2024

SUMMARYOzone (O3) is an oxidative pollutant that significantly threatens plant development and ecological dynamics. The present study explores the impact of O3 on Moringa (Moringa oleifera) ecotypes when exposed to ambient and elevated O3 levels. Elevated O3 concentrations resulted in significant reductions in total biomass for all ecotypes. Photosynthetic parameters, including stomatal conductance (gsto), CO2 assimilation (Pn), and carboxylation efficiency (K), decreased under elevated O3 in some ecotypes, indicating a detrimental effect on carbon assimilation. Nonstructural carbohydrate (NSC) levels in roots varied among ecotypes, with significant reductions in starch content observed under elevated O3, suggesting a potential shift towards soluble sugar accumulation and reallocation for antioxidant defense. Secondary metabolite analysis revealed increased polyphenol production, particularly quercetin derivatives, under elevated O3 in specific ecotypes, highlighting their role in mitigating oxidative stress. Interestingly, the glucosinolate content also varied, with some ecotypes exhibiting increased levels, suggesting a complex regulatory mechanism in response to O3 exposure. The study underscores the intrinsic variability among Moringa ecotypes in response to O3 stress, emphasizing the importance of genetic diversity for adaptation. The findings indicate that Moringa's metabolic plasticity, including shifts in NSC and SM production, plays a crucial role in its defense mechanisms against O3‐induced oxidative stress. These insights are vital for optimizing the cultivation and utilization of Moringa in diverse environmental conditions, particularly in regions with elevated O3 levels.
Identification of two novel variants, c.–35A>T and c.[–35A>T, 725T>G], in the FUT1 gene in a patient exhibiting the para‐Bombay phenotype
Authors: Intharanut Kamphon , Khumsuk Piyathida , Chidtrakoon Sarisa , Glab‐ampai Kantaphon , Nathalang Oytip 
Published: October 2024

AbstractBackgroundReduced or absent H antigens on red cells with the (para‐)Bombay phenotype can arise from FUT1 gene mutations, impacting the structure and function of 1,2‐L‐fucosyltransferase 1 (1,2‐L‐FucT1). Here, we identified the novel mutations in one patient displaying the para‐Bombay phenotype and examined the potential molecular mechanisms underlying this phenotype.Materials and MethodsABH antigens and antibodies were detected in patient's blood and saliva using serological methods. The genotypes of ABO, FUT1, and FUT2 were imputed using the genetic variations discovered in the whole exome sequencing data. Three‐dimensional (3D) models of FUT1 variants were built using Deepmind's AlphaFold2 and HDOCK, and the possible effects of the variants were predicted to evaluate using DynaMut2 and Polyphen‐2.ResultsSerological analysis confirmed the para‐Bombay B phenotype producing anti‐HI and exhibiting normal genotypes ABO*B.01/O.01.02 and FUT2*01.09/01.09. Remarkably, the phenotype is caused by a compound heterozygous genotype: one allele containing the novel c.–35A>T mutation and the known c.725T>G mutation (p.Leu242Arg) of FUT1, and the other allele containing the c.–35A>T mutation. From the computerized stimulation analysis, p.Arg242 of the FUT1 variant may be detrimental, destabilizing, and probably damaging to 1,2‐L‐FucT1, although not altering the 3D structure of the entire enzyme. The c.–35A>T promoter DNA left at the binding interface of both ZID and c‐Rel transcription factors may enable regulation of 1,2‐L‐FucT1 function at gene promoters.ConclusionWe identified the two novel variants, c.–35A>T and c.[−35A>T, 725T>G], in the FUT1 causing the para‐Bombay phenotype. This finding may clarify the molecular mechanisms and enhance blood transfusion safety.
Growth form variations of Amaranthus hybridus L. biotypes with EPSPS mutations
Authors: Schneider Ana B. , Panigo Elisa S. , Dotto Marcela , Cortés Eduardo , Perreta Mariel G. , Dellaferrera Ignacio M. 
Published: October 2024

AbstractSeveral Amaranthus hybridus L. biotypes present single and triple amino acid substitutions in EPSPS (5‐enolypyruvylshikimate‐3‐phosphate synthase). In resistant and tolerant species to glyphosate, differential susceptibility and alterations in the EPSPS sequence could cause modifications in their morphological–structural characteristics, an issue that is unknown for biotypes of A. hybridus. The aim of the work was to determine the susceptibility to glyphosate, the presence of mutations in EPSPS and the growth form in four biotypes of this weed from north‐central Argentina. To carry out this, a dose–response assay was conducted, to determine the presence of mutations in the EPSPS, DNA was extracted, amplified and sequenced, and finally, to evaluate the morphological–structural variations, the growth form was analysed. Our findings revealed that Ah03, Ah07 and Ah36 biotypes had resistance factors of 22 to 118. The EPSPS sequence remains unaltered in the Ah39 biotype, leading it to be considered a susceptible biotype. Biotypes Ah03 and Ah36 had heterozygous changes in the DNA sequence resulting in amino acid substitutions at positions 102, 103 and 106 being considered resistant biotypes, whereas in Ah07 some individuals showed unaltered DNA sequences and others exhibited heterozygous changes. The growth form was quantitatively different between biotypes, standing the most susceptible biotype that produced more seeds and required less thermal time for the appearance of the terminal inflorescence. The present investigation provides new evidence that resistant biotypes with the triple DNA mutation exhibit variations in the growth form compared to susceptible biotypes of this weed.
Single-cell transcriptional analysis of murine norovirus infection in a human intestinal cell line
Authors: Matsushima Yuki , Levenson Eric A. , Chaimongkol Natthawan , Harris Loyall , Zhao Yongmei , Turan Sevilay , Otaizo-Carrasquero Francisco , Ganesan Sundar , Hornick Katherine M. , Martens Craig , Sosnovtsev Stanislav V. , Green Kim Y. 
Published: October 2024

ABSTRACT

Noroviruses are a major agent of acute gastroenteritis in humans, but host cell requirements for efficient replication
in vitro
have not been established. We engineered a human intestinal cell line (designated mCD300lf-hCaco2) expressing the murine norovirus (MNV) receptor, mouse CD300lf to become fully permissive for MNV replication. To explore the replicative machinery and host response of these cells, we performed a single-cell RNA sequencing (scRNA-seq) transcriptomics analysis of an MNV infection over time. Marked similarities were observed between certain global features of MNV infection in human cells compared to those previously reported in mouse cells by whole population transcriptomics such as downregulation of ribosome biogenesis, mitochondrial dysfunction, and cell cycle preference for G1. Our scRNA-seq analysis allowed further resolution of an infected cell population into distinct clusters with varying levels of viral RNA and interferon-stimulated gene ISG15 transcripts. Cells with high viral replication displayed downregulated ribosomal protein small (RPS) and large (RPL) genes and mitochondrial complexes I, III, IV, and V genes during exponential viral propagation. Ferritin subunit genes FTL and FTH1 were also downregulated during active MNV replication, suggesting that inhibition of iron metabolism may increase replication efficiency. Consistent with this, transcriptional activation of these genes with ferric ammonium citrate and overexpression of FTL lowered virus yields. Comparative studies of cells that support varying levels of norovirus replication efficiency, as determined by scRNA-seq may lead to improved human cell-based culture systems and effective viral interventions.

IMPORTANCE
Human noroviruses cause acute gastroenteritis in all age groups. Vaccines and antiviral drugs are not yet available, in part, because it is difficult to propagate the viruses causing human disease in standard laboratory cell culture systems. In contrast, a norovirus found in mice [murine norovirus (MNV)] replicates efficiently in murine-based cell culture and has served as a model system. In this study, we established a new human intestinal cell line that was genetically modified to express the murine norovirus receptor so that the human cells became permissive to murine norovirus infection. We then defined the host response to MNV infection in the engineered human cell line at a single-cell resolution and identified cellular genes associated with the highest levels of MNV replication. This study may lead to the improvement of the current human norovirus cell culture systems and help to identify norovirus-host interactions that could be targeted for antiviral drugs.