bluuebunny/crossref_metadata_2025_split huggingface dataset:
Spatial Variation of the Native Colon Microbiota in Healthy Adults
Authors: Flynn Kaitlin J. , Ruffin Mack T. , Turgeon D. Kim , Schloss Patrick D. 
Published: April 2018

Abstract
The microbiome has been implicated in the development of colorectal cancer and inflammatory bowel diseases. The specific traits of these diseases vary along the axis of the digestive tract. Further, variation in the structure of the gut microbiota has been associated with both diseases. We profiled the microbiota of the healthy proximal and distal mucosa and lumen to better understand how bacterial populations vary along the colon. We used a two-colonoscope approach to sample proximal and distal mucosal and luminal contents from the colons of 20 healthy subjects that had not undergone any bowel preparation procedure. The biopsies and home-collected stool were subjected to 16S rRNA gene sequencing, and random forest classification models were built using taxa abundance and location to identify microbiota specific to each site. The right mucosa and lumen had the most similar community structures of the five sites we considered from each subject. The distal mucosa had higher relative abundance of Finegoldia, Murdochiella, Peptoniphilus, Porphyromonas, and Anaerococcus. The proximal mucosa had more of the genera Enterobacteriaceae, Bacteroides, and Pseudomonas. The classification model performed well when classifying mucosal samples into proximal or distal sides (AUC = 0.808). Separating proximal and distal luminal samples proved more challenging (AUC = 0.599), and specific microbiota that differentiated the two were hard to identify. By sampling the unprepped colon, we identified distinct bacterial populations native to the proximal and distal sides. Further investigation of these bacteria may elucidate if and how these groups contribute to different disease processes on their respective sides of the colon. Cancer Prev Res; 11(7); 393–402. ©2018 AACR.
Diagnostic Value of Gains and Corrective Saccades in Video Head Impulse Test in Vestibular Neuritis
Authors: Yang Chan Joo , Cha Eun Hye , Park Jun Woo , Kang Byung Chul , Yoo Myung Hoon , Kang Woo Suk , Ahn Joong Ho , Chung Jong Woo , Park Hong Ju 
Published: April 2018

ObjectivesWe investigated changes in video head impulse test (vHIT) gains and corrective saccades (CSs) at the acute and follow‐up stages of vestibular neuritis to assess the diagnostic value of vHIT.Study DesignCase series with chart review.SettingTertiary medical center.Subjects and MethodsSixty‐three patients with vestibular neuritis who underwent vHIT at an initial presentation and an approximately 1‐month follow‐up were included. vHIT gains, gain asymmetry (GA), peak velocities of CS, and interaural difference of CS (CSD) were analyzed.ResultsMean vHIT gains increased significantly from the acute stage to the follow‐up exam. The mean GA, peak velocities of CS, and CSD had decreased significantly at the follow‐up. The incidence of CSs was also significantly decreased at the follow‐up. The abnormal rate (87%) considering both gain and CS value was significantly higher than that (62%) considering vHIT gain only at the follow‐up, although the abnormal rates did not differ at the acute stage (97% vs 87%).ConclusionThe abnormal rates based on both vHIT gains and CS measurements are similar at the acute stage of VN but are considerably higher at the follow‐up stage compared with the abnormal rates based on vHIT gains alone. It is thus advisable to check both CS and vHIT gain while performing vHIT to detect vestibular hypofunction.
Differential Gene Expression and Pathway Analysis in Juvenile Nasopharyngeal Angiofibroma Using RNA Sequencing
Authors: Jones Joel W. , Usman Shireen , New Jacob , Holcomb Andrew , Gunewardena Sumedha , Tawfik Ossama , Hoover Larry , Bruegger Daniel , Thomas Sufi Mary 
Published: April 2018

Juvenile nasopharyngeal angiofibroma (JNA) is a highly vascularized and locally aggressive tumor that typically presents in adolescent males. The molecular biology of this tumor remains understudied. We sought to identify differentially expressed genes in the JNA transcriptome through messenger RNA sequencing of primary fibroblasts from 2 tumor explants and tonsil tissue from tumor‐free subjects. In total, 1088 significant, differentially expressed genes were identified with 749 upregulated and 339 downregulated. Pathway analysis identified a number of activated signaling pathways, most notably, the vascular endothelial growth factor (VEGF) pathway (adjusted overlap P =. 03). VEGF‐A showed a 4.4‐fold upregulation in JNA samples. In addition, the angiogenic receptor, fibroblast growth factor receptor 2 (FGFR2), was not present in tumor‐free samples but increased in JNA. We validate these findings with immunohistochemistry, demonstrating upregulation of VEGF and FGFR2 in patient sections. Inhibition of the VEGF or FGFR signaling axes may have therapeutic potential in the treatment of JNA.
Role of Active Versus Passive Complementary and Integrative Health Approaches in Pain Management
Authors: Cosio David , Lin Erica 
Published: April 2018

Background A general conclusion about the treatment of chronic, noncancer pain is that the results from traditional, passive modalities are disheartening. Perhaps this may be due to the propensity of patients to seek out passive versus active treatments. In pain management, active treatments should be the primary focus, with passive interventions as an adjunct. Objective The current study tested the hypotheses that Veterans would report a greater significant increase in active versus transitional and active versus passive complementary and integrative health (CIH) utilization after completing a formal pain education program. Methods The current study is a secondary analysis of existing data from an original study. The current study used a quasi-experimental, 1-group, pre-/posttest design. One hundred three Veterans completed a 12-week, “Pain Education School” program at a Midwestern VA Medical Center between November 4, 2011, and October 26, 2012. As part of the introduction and conclusion of the program, all Veterans completed a pre- and posteducation assessment which included an adaptation of the Complementary and Alternative Medicine Questionnaire©, SECTION A: Use of Alternative Health Care Providers measure. Results Significant differences were found between the pre- and posttest measures of use of active ( P = .000) ( p<.001), transitional ( P = .011), and passive ( P = .007) CIH modalities. Conclusion The current findings suggest that an educational intervention in conjunction with the availability of treatment options has the potential to increase the use of those treatments. The current pain education program also seems to be aligned with the goal of pain self-management, which is to utilize more active interventions as a primary therapy.
Patient Perspectives of Midlevel Providers in Orthopaedic Sports Medicine
Authors: Manning Blaine T. , Bohl Daniel D. , Hannon Charles P. , Redondo Michael L. , Christian David R. , Forsythe Brian , Nho Shane J. , Bach Bernard R. 
Published: April 2018

Background: Midlevel providers (eg, nurse practitioners and physician assistants) have been integrated into orthopaedic systems of care in response to the increasing demand for musculoskeletal care. Few studies have examined patient perspectives toward midlevel providers in orthopaedic sports medicine. Purpose: To identify perspectives of orthopaedic sports medicine patients regarding midlevel providers, including optimal scope of practice, reimbursement equity with physicians, and importance of the physician’s midlevel provider to patients when initially selecting a physician. Study Design: Cross-sectional study; Level of evidence, 3. Methods: A total of 690 consecutive new patients of 3 orthopaedic sports medicine physicians were prospectively administered an anonymous questionnaire prior to their first visit. Content included patient perspectives regarding midlevel provider importance in physician selection, optimal scope of practice, and reimbursement equity with physicians. Results: Of the 690 consecutive patients who were administered the survey, 605 (87.7%) responded. Of these, 51.9% were men and 48.1% were women, with a mean age of 40.5 ± 15.7 years. More than half (51.2%) perceived no differences in training levels between physician assistants and nurse practitioners. A majority of patients (62.9%) reported that the physician’s midlevel provider is an important consideration when choosing a new orthopaedic sports medicine physician. Patients had specific preferences regarding which services should be physician provided. Patients also reported specific preferences regarding those services that could be midlevel provided. There lacked a consensus on reimbursement equity for midlevel practitioners and physicians, despite 71.7% of patients responding that the physician provides a higher-quality consultation. Conclusion: As health care becomes value driven and consumer-centric, understanding patient perspectives on midlevel providers will allow orthopaedic sports medicine physicians to optimize efficiency and patient satisfaction. Physicians may consider these data in clinical workforce planning, as patients preferred specific services to be physician or midlevel provided. It may be worthwhile to consider midlevel providers in marketing efforts, given that patients considered the credentials of the physician’s midlevel provider when initially selecting a new physician. Patients lacked consensus regarding reimbursement equity between physicians and midlevel providers, despite responding that the physician provides a higher-quality consultation. Our findings are important for understanding the midlevel workforce as it continues to grow in response to the increasing demand for orthopaedic sports care.
Interstitial lung disease is associated with an increased risk of lung cancer in systemic sclerosis: Longitudinal data from the Canadian Scleroderma Research Group
Authors: Sakr Lama , Hudson Marie , Wang Mianbo , Younanian Elie , Baron Murray , Bernatsky Sasha ,   Canadian Scleroderma Research Group
Published: April 2018

Objective: The literature supports an increased risk of malignancy in systemic sclerosis, including lung cancer. Our objective was to identify potential independent predictors of lung cancer risk in systemic sclerosis. Methods: We used a cohort of 1560 systemic sclerosis patients from the Canadian Scleroderma Research Group, enrolled from 2004 and followed for a maximum of 11 years. Time to lung cancer was calculated from the onset of the first non-Raynaud’s symptoms. Baseline demographic, clinical, and serological characteristics of patients with and without lung cancer were compared. Cox proportional hazards models were used to estimate the effects of demographic variables, exposure to smoking, disease duration, disease subset (diffuse vs limited), immunosuppressant drug exposure, and presence of interstitial lung disease on the risk of lung cancer. Results: Over the 5519 total person-years of follow-up, 18 SSc patients were diagnosed with lung cancer after cohort entry (3.2 cancers per 1000 person-years). In univariate comparisons, cancer cases were more likely to be male, to have a smoking history, and to have interstitial lung disease than non-cases. In multivariate analysis, interstitial lung disease was independently associated with the risk of lung cancer (hazard ratio: 2.95, 95% confidence interval: 1.10–7.87). Conclusion: In addition to known demographic (male sex) and lifestyle risk factors (smoking), interstitial lung disease is an independent risk factor for lung cancer in systemic sclerosis. These results have implications for lung cancer screening in systemic sclerosis.
Surgical Repair of a Persistent Full-Thickness Macular Fold With Base-to-Base Photoreceptor Apposition Secondary to Hypotony From Trabeculectomy Surgery and Postoperative Laser Suturelysis
Authors: Iaboni Douglas S. M. , Seamone Mark E. , Gupta R. Rishi 
Published: April 2018

Purpose: To report the successful repair of a persistent full-thickness retinal fold secondary to hypotony from trabeculectomy surgery. Results: Laser suturelysis was performed on a patient to relieve an elevated intraocular pressure posttrabeculectomy surgery. This resulted in chronic hypotony, a full-thickness retinal fold, and a decline in visual acuity to “count fingers.” The patient underwent a pars plana vitrectomy with internal limiting membrane peeling, subretinal injection of balanced saline solution, fluid-air exchange, injection of F-Decalin, peripheral retinotomy, endolaser photocoagulation, and vitreous substitution with 15% C3F8 gas. Anatomic improvement was documented via multimodal imaging. Six months postoperatively best-corrected visual acuity (BCVA) returned to 20/30. Conclusions: We describe a surgical approach to full-thickness retinal folds and review the management options previously reported in the setting of hypotony. We provide support for the active management of appositional full-thickness retinal folds involving the fovea and demonstrate that surgical correction can significantly improve BCVA even after 4 months of hypotony.
Progesterone as a Postnatal Prophylactic Agent for Encephalopathy Caused by Prenatal Hypoxic Ischemic Insult
Authors: Kawarai Yoshimasa , Tanaka Hirokazu , Kobayashi Tatsuya , Shozu Makio 
Published: April 2018

Abstract
Brain damage caused by hypoxic ischemic insult during the perinatal period causes hypoxic ischemic encephalopathies (HIEs). Therapeutic hypothermia is indicated for HIE, but because the therapeutic burden is large for its limited therapeutic effectiveness, another strategy is needed. Progesterone (P4) plays a neuroprotective role through the actions of its metabolite, allopregnanolone (Allo), on P4 receptor, γ-aminobutyric acid type A receptors or both. We examined the therapeutic potential of P4 using a newborn rat model of HIE. Fetal rats were exposed to transient ischemic hypoxia by 30-minute bilateral uterine artery clamping on gestational day 18. After spontaneous birth, newborn pups were subcutaneously injected with P4 (0.10 or 0.01 mg), medroxyprogesterone acetate (MPA; 0.12 mg), or Allo (0.10 mg) through postnatal days (PDs) 1 to 9. Brain damage in the rats was assessed using the rotarod test at PD50. The HIE insult reduced the rats’ ability in the rotarod task, which was completely reversed by P4 and Allo, but not by MPA. Histological examination revealed that the HIE insult decreased neuronal (the cortex and the hippocampal CA1 region) and oligodendroglial cell density (the corpus callosum) through PD0 to PD50. The axon fiber density and myelin sheath thickness in the corpus callosum were also reduced at PD50. The time-course study revealed that P4 restored oligodendroglial cells by PD5, which was followed by neuroprotective action of P4 that lasted long over the injection period. These results suggest that P4 protects the neonatal brain from HIE insult via restoration of oligodendroglial cells.
The DEAD-box RNA helicase Ddx39ab is essential for myocyte and lens development in zebrafish
Authors: Zhang Linlin , Yang Yuxi , Li Beibei , Scott Ian C. , Lou Xin 
Published: April 2018

ABSTRACT
RNA helicases from the DEAD-box family are found in almost all organisms and have important roles in RNA metabolism, including RNA synthesis, processing and degradation. The function and mechanism of action of most of these helicases in animal development and human disease remain largely unexplored. In a zebrafish mutagenesis screen to identify genes essential for heart development we identified a mutant that disrupts the gene encoding the RNA helicase DEAD-box 39ab (ddx39ab). Homozygous ddx39ab mutant embryos exhibit profound cardiac and trunk muscle dystrophy, along with lens abnormalities, caused by abrupt terminal differentiation of cardiomyocyte, myoblast and lens fiber cells. Loss of ddx39ab hindered splicing of mRNAs encoding epigenetic regulatory factors, including members of the KMT2 gene family, leading to misregulation of structural gene expression in cardiomyocyte, myoblast and lens fiber cells. Taken together, these results show that Ddx39ab plays an essential role in establishment of the proper epigenetic status during differentiation of multiple cell lineages.
A model suite of green algae within the Scenedesmaceae for investigating contrasting desiccation tolerance and morphology
Authors: Cardon Zoe G. , Peredo Elena L. , Dohnalkova Alice C. , Gershone Hannah L. , Bezanilla Magdalena 
Published: April 2018

ABSTRACT
Microscopic green algae inhabiting desert microbiotic crusts are remarkably diverse phylogenetically, and many desert lineages have independently evolved from aquatic ancestors. Here we worked with five desert and aquatic species within the family Scenedesmaceae to examine mechanisms that underlie desiccation tolerance and release of unicellular versus multicellular progeny. Live cell staining and time-lapse confocal imaging coupled with transmission electron microscopy established that the desert and aquatic species all divide by multiple (rather than binary) fission, although progeny were unicellular in three species and multicellular (joined in a sheet-like coenobium) in two. During division, Golgi complexes were localized near nuclei, and all species exhibited dynamic rotation of the daughter cell mass within the mother cell wall at cytokinesis. Differential desiccation tolerance across the five species, assessed from photosynthetic efficiency during desiccation/rehydration cycles, was accompanied by differential accumulation of intracellular reactive oxygen species (ROS) detected using a dye sensitive to intracellular ROS. Further comparative investigation will aim to understand the genetic, ultrastructural and physiological characteristics supporting unicellular versus multicellular coenobial morphology, and the ability of representatives in the Scenedesmaceae to colonize ecologically diverse, even extreme, habitats.
Postponing surgery of paediatric supracondylar humerus fractures to office hours increases consultant attendance in operations and saves operative room time
Authors: Tuomilehto N. , Sommarhem A. , Salminen P. , Nietosvaara A. Y. 
Published: April 2018

Purpose To assess if postponing surgery of paediatric supracondylar humerus fractures (SCHF) without compromised blood circulation to office hours can improve the quality of reduction and pin fixation and decrease complications. Methods In 2004, night-time (0am to 7am) surgery was allowed only for children with compromised blood circulation. Number of open reductions, surgeons experience, operation time, quality of reduction (Baumann angle, anterior humeral line crossing point with capitellum) and pin fixation as well as the number of complications were compared in 100 children before (A) and 100 after (B) the new protocol. Surgery was commenced during office hours (8am to 3pm) in 27% (A) versus 55% (B) and delay to surgery from admission exceeded six hours in 25% (A) versus 52% (B) of the children. Results Open reduction was performed in eight (A) versus 11 (B) children. In group A, 40% were operated on by a registrar alone compared with 14% in group B. Mean operation room time decreased by 11 minutes in group B. Radiographic alignment was satisfactory at fracture union in 68% (A) versus 68% (B) and radiologically stable pin fixation in 42% (A) versus 55% (B) of children (p = 0.08). The was no statistical difference in admission time, re-reductions, infections, permanent iatrogenic nerve injuries or corrective osteotomies between the patient groups. Conclusions No statistical difference in quality of reduction or pin fixation or in number of complications was observed. Postponing operative treatment of SCHF without vascular compromise increased consultant attendance in operations and decreased operative room time. Level of Evidence III - retrospective comparative study
Webtoons to promote critical thinking in neo-liberal South Korea: A thematic analysis of Awl social justice themes
Authors: Choi Jinhee 
Published: April 2018

Abstract
This article explores the use of South Korean webtoons, Songkot (meaning Awl), for informal adult learning through visual narratives and audience comments in the social, historic and academic context. Drawing on Newman’s critical thinking and Giroux’s critical public pedagogy, the study explicates how the Awl creates a discursive space for an unpublicized theme, unionization, through its narrative structures and audience discussion. The findings imply that Awl’s major themes – critical thinking, empathy and social action – create informal adult learning opportunities for the public by increasing their conscious awareness of institutionalized oppressions. Awl also has the potential to promote critical thinking about commonly unvoiced issues of social justice in neo-liberal South Korea.
Marketing anime to a global audience: A paratextual analysis of promotional materials from Spirited Away
Authors: Carter Laz 
Published: April 2018

Abstract
This article will focus specifically on the marketing materials utilized in the Japanese and American markets for Sen to Chihiro no kamikakushi (Spirited Away) (Miyazaki, 2001). That is to say, it takes a more in-depth look at the paratextual deployment of film posters and theatrical trailers. Building on the work of Rayna Denison, Keith Johnston and Eriko Ogihara-Schuck, this study delves deeper into the specific paratextual elements present within Spirited Away’s promotional ephemera. Focusing on two primary factors – branding and linguistics – the following campaign analysis examines the meaning behind the addition and elision of certain signifiers. By comparing the different versions of both posters and trailers, this article highlights areas of cultural difference, postulating that the anglophonic paratexts have undergone a process of Disneyfication. Finally, the author extrapolates the key selling points that are accentuated for both the domestic and global markets of Studio Ghibli films and then muses on the resulting hierarchy of brand networks that appear to have formed.
The ‘Totoro Meme’ and the politics of transfandom pleasure
Authors: Morimoto Lori 
Published: April 2018

Abstract
This article is an exploration of the ‘Totoro meme’ as a site of affective, transfandom pleasure. In the Totoro meme, Japanese and non-Japanese fans alike appropriate the now-iconic image of Satsuki, Mei and an umbrella-toting Totoro at a bus stop from Hayao Miyazaki’s 1988 film, Tonari no Totoro (My Neighbor Totoro), to their own fannish ends, creating fan art that inserts favourite characters from other media into the scene in ways that often have a doubled semiotic resonance. I argue that this meme is characteristic not of the global appropriation of a broad ‘Japanese anime style’, per se, but a specific, affectively appealing ‘Ghibli style’, one that is fully part of non-Japanese fans’ own popular cultural repertoires. In its cross-border merging of globally circulating Studio Ghibli aesthetics with other fan-favourite media, I contend that the Totoro meme and its associated fanworks are in fact wholly congruent with, and representative of, what Matt Hills has termed ‘trans-fandom’ (2015), contemporary practices of ‘navigating across and combining and fusing fandoms’ (Hills 2015: 159). I conclude with a consideration of the implications of what might be termed ‘corporate transfandom’ in the context of transfannish citations of Kaze no tani no Naushika (Nausicaä of the Valley of the Wind) (Miyazaki, 1984) in Star Wars: The Force Awakens (Abrams, 2015).