bluuebunny/crossref_metadata_2025_split huggingface dataset:
TRANSFORMASI GENETIK DAN EKSPRESI MUTAN <em>SUCROSE PHOSPHATE SYNTHASE</em> PADA TANAMAN TOMAT
Authors: Fibriani Suwinda , Agustien Inyana Dwi , Sawitri Widhi Dyah , Sugiharto Bambang 
Published: August 2019

Genetic Transformation and Expression of Sucrose Phosphate Synthase Mutant in Tomato Plant ABSTRACTSucrose phosphate synthase (SPS) is a key enzyme responsible for sucrose biosynthesis. In its regulation, SPS activity is modulated by an allosteric effector glucose-6-phosphate (G6P) suggested to have an ability to bind SPS N-terminus domain. To understand the role of N-terminus in regulating SPS, the SPS gene was mutated with the deletion of N-terminus domain (∆N-SPS). The ∆N-SPS gen was transformed into tomato plants with 5% transformation efficiency. Three transgenic tomato plant 4.20, 5.5.1, and 5.10 were obtained and confirmed by PCR analysis. Transgenic tomato expression was characterized by enzymatic analysis. Result showed that the G6P allosteric regulation in transgenic ∆N-SPS had lost and the SPS activity increased by 2-fold compared to non-transgenic plant. This showed that N-terminus domain-deleted SPS could be actively expressed in plant. Keywords: enzyme, genetic transformation, N-terminus domain deletion, sucrose phosphate synthase, tomato ABSTRAKSucrose phosphate synthase (SPS) merupakan enzim kunci yang bertanggung jawab dalam sintesis sukrosa. Dalam regulasinya, aktifitas SPS dipengaruhi oleh alosterik efektor glukosa-6-fosfat (G6P) yang diduga dapat berikatan pada domain N-terminus SPS. Untuk mengetahui peran N-terminus pada regulasi SPS, dilakukan mutasi SPS dengan penghilangan domain N-terminus (∆N-SPS). Gen ∆N-SPS diinsersi pada tanaman tomat melalui transformasi genetik dengan efisiensi transformasi 5%. Tiga tanaman transgenik tomat (event4.20; 5.5.1; dan 5.10) didapatkan dan positif terkonfirmasi melalui analisis PCR. Ekspresi mutan dikarakterisasi melalui analisis enzimatik. Hasil menunjukkan bahwa tanaman tomat transgenik ∆N-SPS tidak dipengaruhi regulasi alosterik G6P dan aktifitas SPS 2 kali lipat lebih tinggi daripada tanaman bukan transgenik. Ini menunjukkan bahwa SPS dengan delesi domain N-terminus dapat terekspresi aktif pada tanaman. Kata Kunci: delesi domain N-terminus, enzim, sucrose phosphate synthase, tomat, transformasi genetik
EMBRIOGENESIS SOMATIK <em>IN VITRO</em> DAN REGENERASI PLANLET DARI TIGA VARIETAS ALFALFA (<em>Medicago sativa</em> L.)
Authors: Sulastri Sulastri , Nawfetrias Winda , Pinardi Djatmiko , Rosdayanti Henti 
Published: August 2019

In Vitro Somatic Embryogenesis and Plantlet Regeneration of Three Varieties of Alfalfa (Medicago sativa L.)ABSTRACTAlfalfa (Medicago sativa L.) is a valuable plant as a source of food for animal, forage, pharmaceutical, medicine, food supplement, and human consumption. In vitro selection technology combined with induction or spontaneous mutagenesis has been effective in altering or isolating genetic variability for desirable characters. Consequently, a reproducible in vitro propagation technique of that plant is mandatory. The aim of the research was to obtain information on the embryogenic callus induction, somatic embryogenesis, and plantlet regeneration of three varieties of alfalfa. The results showed that an optimum embryogenic callus induction (82%) was obtained on Murashige & Skoog (MS) basal medium containing 2 ppm 2,4-dichlorophenoxyacetic acid (2,4-D), 2 ppm kinetin and 2 ppm a-naphthaleneacetic acid (NAA). Those embryogenic calli could subsequently develop into somatic embryos, which germinated and regenerated into normal plantlets on R1 medium consisting of MS nutrients without the addition of plant growth regulator.Keywords: alfalfa, callus, embryogenic, plantlets, regeneration ABSTRAKAlfalfa (Medicago sativa) adalah tanaman berharga sebagai sumber makanan untuk hewan, yaitu hijauan pakan ternak, farmasi, obat-obatan, suplemen makanan dan konsumsi manusia. Teknologi seleksi in vitro yang dikombinasikan dengan induksi atau mutagenesis spontan telah terbukti efektif dalam mengubah atau mengisolasi variabilitas genetik untuk karakter yang diinginkan. Oleh sebab itu, keberhasilan teknik perbanyakan in vitro yang telah terbukti dapat direproduksi dari tanaman tersebut menjadi syarat yang harus terpenuhi. Tujuan dari penelitian ini adalah untuk mendapatkan informasi mengenai induksi kalus embriogenik, embriogenesis somatik dan regenerasi planlet dari tiga varietas alfalfa. Hasil penelitian menunjukkan bahwa induksi kalus embriogenik optimal (82%) didapat pada media Murashige & Skoog (MS) dengan 2 ppm 2,4-dichlorophenoxyacetic acid (2,4-D), 2 ppm kinetin dan 2 ppm a-naphthaleneacetic acid (NAA). Kalus embriogenik tersebut dapat membentuk embrio somatik, embrionya berkecambah dan beregenerasi membentuk planlet normal pada perlakuan media R1 yaitu nutrisi MS tanpa penambahan zat pengatur tumbuh.Kata Kunci: alfalfa, embriogenik, kalus, planlet, regenerasi
PENGARUH TEKNIK PENGAPUNGAN HAYATI MELALUI FERMENTASI <em>Rhizopus</em> sp. TERHADAP KANDUNGAN NUTRISI PAKAN IKAN APUNG
Authors: Paramadini Sabrina Ayu , Sriherwanto Catur , Nurlaila . , Suja'i Imam , Annisa Mutiara Ayu 
Published: August 2019

Influence of Biofloatation Technique through Rhizopus sp. Fermentation on the Nutritional Quality of the Floating Fish FeedABSTRACTSolid fermentation using the mold Rhizopus has been used as an alternative method to improve the physical quality of fish feed, namely stability in water and floatability. Although the fermented fish feed produced had been shown previously to have better stability in water and floatability, the effect of the fermentation on the fish feed nutrition has not yet been known. This study aimed to determine the effect of solid fermentation using the mold Rhizopus sp. on the nutrient content of the fermented feed. In addition, the effect of adding tapioca as much as 0, 1, 2, 3, and 4 g to the dry weight loss and density of the fermented feed was investigated. The results showed that the fermented feed contained higher levels of ash and protein than that before fermentation. The addition of tapioca up to 4 g had no significat effect (p>0.01) on the dry weight loss of the fermented feed, but tended to increase its density compared to those without tapioca addition.Keywords: density, fermentation, fish feed, nutrition, Rhizopus ABSTRAKFermentasi padat menggunakan kapang Rhizopus telah digunakan sebagai metode alternatif untuk memperbaiki kualitas fisik pakan ikan, yakni stabilitas dalam air dan daya apung. Meskipun pakan ikan fermentasi yang dihasilkan sebelumnya sudah dibuktikan memiliki stabilitas dalam air dan daya apung yang lebih baik, namun pengaruh fermentasi terhadap nutrisi pakan ikan tersebut belumlah diketahui. Penelitian ini bertujuan mengetahui pengaruh fermentasi padat menggunakan kapang Rhizopus sp. terhadap kandungan nutrisi pakan fermentasi, dan pengaruh penambahan tapioka sebanyak 0, 1, 2, 3, dan 4 g terhadap kehilangan berat kering dan mass jenis pakan hasil fermentasi. Hasilnya menunjukkan bahwa pakan fermentasi mengandung kadar abu dan protein lebih tinggi dibandingkan sebelum difermentasi. Penambahan tapioka hingga 4 g tidak berpengaruh nyata (p>0,01) pada kehilangan berat kering pakan fermentasi, namun cenderung meningkatkan massa jenis dibandingkan tanpa penambahan tapioka.Kata kunci: fermentasi, massa jenis, nutrisi, pakan ikan, Rhizopus
The Trouble With Think Alouds: Generating Data Using Concurrent Verbal Protocols
Authors: Branch Jennifer L. 
Published: August 2019

Verbal protocol analysis is a methodology that is frequently used in cognitive psychology and education. The use of this method in library and information studies, however, is still very limited. Verbal protocol analysis aims to find cognitive processes while solving a problem. However, concurrent verbal protocols have been seen to cause problems when the task involves a high cognitive load, when the information is difficult to verbalize because of its form, i.e., visual data, or when the processes are automatic for the participants. This paper looks at studies using concurrent verbal protocols and summarizes the findings of this research. Then, this paper compares and contrasts the analysis of 130 concurrent verbal protocols (Think Alouds) gathered from twelve junior high school students from Inuvik, Canada. These Think Alouds are from a case study of the information-seeking processes of junior high students when accessing information from CD-ROM encyclopedias. Preliminary analysis indicates that several of the participants experienced difficulty with Think Alouds. A discussion of possible reasons for these difficulties will be included.
Rancang Bangun Portable Monitoring Suhu Lingkungan Berbasis Internet Of Things
Authors: Suryo Yoedo Ageng 
Published: August 2019

Abstrak— Perancangan sistem monitoring suhu yang portable akan menjawab kebutuhan akuisisi data yang tidak terbatas tempat dan waktu. Ketergantungan terhadap sumber daya menjadi faktor penentu utama portabilitas sistem yang berbasis internet of things. Rancangan penelitian ini merupakan aktifitas monitoring suhu lingkungan dalam satuan Celcius yang bisa ditempatkan di manapun. Melibatkan komponen utama sebagai penunjang aktifitas monitoring berupa sensor suhu untuk mendapatkan data, mikrokontroler sebagai pengontrol kerja sistem dan GSM/GPRS serial modem sebagai modul komunikasi data. Pencatatan data berupa besaran suhu analog yang dikonversi dengan kenaikan 10mV/oC dengan tingkat akurasi sebesar 0.5oC. Pencatatan data hasil monitoring berpusat di server Thing speak yang dilakukan secara realtime. Pengguna dapat mengolah data yang diperlukan untuk keperluan analisis lebih lanjut. Berdasarkan perancangan sistem ini, didapatkan kemampuan untuk mengukur suhu dari 6oC sampai 100oC dengan error rata-rata sebesar 0.023oC. Kata kunci : portable, internet of things, monitoring, Thingspeak, realtime Abstract— The design of a portable temperature monitoring system responses data acquisition that are not limited to time and place. Dependence on resources is the main issue of system portability based on internet of things. The design of this research is environmental temperature monitoring in Celsius which can be placed anywhere. Involving the main components as supporting monitoring activities like temperature sensor to obtain data, a microcontroller as the controller and GSM/GPRS serial modem as a data communication module. Recording data in the form of analog temperature quantities that are converted with an increase of 10mV/oC with an accuracy of 0.5oC. The recording of monitoring data results is concentrated on the Thingspeak server which is done in realtime. User can process the data for further analysis. Based on the design of this system, it was obtained the ability to measure temperatures from 6oC to 100oC with an average error of 0.023oC. Keywords : portable, internet of things, monitoring, Thingspeak, realtime
PENGETAHUAN IBU PREMENOPAUSE DENGAN KESIAPAN MENGHADAPI MENOPAUSE DI DUSUN GAMPING KIDUL AMBARKETAWANG GAMPING SLEMAN
Authors: Sagitawening Hardhini , Susanti Dwi 
Published: August 2019

Background: Premenopause is a transitional stage of fertile period leading to the absence of fertilization 4-5 years before menopause. The need for readiness for premenopausal women will affect anxiety in the face of menopause. One of the factors of readiness is knowledge that serves to find out various problems during menopause.
Objective: To know the relationship of premenopausal women knowledge with readiness to face menopause at Gamping Kidul, Ambarketawang, Gamping sub district, Sleman Yogyakarta.
Methods: Type of quantitative research with cross sectional approach. The population of this study were all premenopausal women aged 45-50 years. The sampling technique used cluster and purposive sampling with research subject of 57 responden. The measuring instrument used was a questionnaire, univariate and bivariate analysis used Kendall's Tau.Results: Good knowledge of premenopausal women 29.8%, sufficient knowledge was 56.1% and lack of knowledge was 14.0% while the category ready 86.0% and not ready 14.0%. The results of statistical tests showed a significant relationship between premenopausal women knowledge and readiness to face menopause (r = 0.647 and p = 0,000).
Conclusion : There is a relationship between premenopausal women knowledge with readiness to face menopause at Gamping Kidul, Ambarketawang, Gamping sub district, Sleman Yogyakarta.

Keywords: Knowledge, premenopause, readiness
PELATIHAN COMPRESSION ONLY-CPR EFEKTIF MENINGKATKAN KETEPATAN KOMPRESI DADA PADA ORANG AWAM
Authors: Nofiyanto Muhamat , Darussalam Miftafu , Setiawan Arif Adi 
Published: August 2019

Background: Many studies show a low rate of immediate chest compression in OHCA cases. One of the factors that inhibits is the lack of knowledge of lay people who witness cases of cardiac arrest.
Objective: To determine the effect of providing compression-only CPR training on chest compression skills in lay people
Methods: This research used pre-experiment with one group pre-post test design, with a total of 28 respondents, using simple random sampling technique. Respondents measured chest compression skills before training, then provided training for 120 minutes.Wilcoxon test was used to analyze data sets.
Results: There were almost the same number between male and female respondents, and most have never received CPR training. Only 5 respondents had received previous training, with details of 3 years, 6 years, 8 years, 9 years and 11 years ago. There was a difference in the accumulation of the accuracy of chest compression before and after training with a p-value of 0,000. Conclusion: Compression only-CPR training can improve chest compression skills, especially aspects of accuracy of compression. Broader training and intensification of training programs are needed to realize emergency preparedness in the campus community.

Keywords: Cardiac arrest, CPR, training
An Analysis of Jargon Used in Political Website
Authors: Syafitri Dewi , Ikhsan M 
Published: August 2019

The objectives of the study were to describe the forms, meanings, and functions of Political Jargon Which were found in Political Website. This study mainly aimed to describe the jargon used in political news of CNN politics as the Political Website. The writers used a qualitative descriptive method in classifying and analyzing the data. After investigating the forms, meanings, and functions of jargon in CNN politics on 1st -30th May, 2016, the writers found several findings. The political jargon in this website can be analyzed in forms were 53 words, 15 phrases, 5 abbreviations and there was no acronym, in the lexical meanings categories there were 58 denotative meanings and 15 connotative meanings from 73 data and the functions of Political Jargon were a belongings to a specific group, to communicate and signal for identification of political group. In conclusion, the jargon used to convey the meaning and to communicate to the readers, in this context as a signal in politics fields. So, the readers will be familiar in using some terms of political jargon in everyday speech.
Keywords: Jargon, Political Website, Meaning
APLIKASI SISTEM PAKAR DIAGNOSA PENYAKIT LAMBUNG MENGGUNAKAN FORWARD CHAINING
Authors: Setiadi Ahmad , Yunita Yunita , Nugroho Ibung Prasetio 
Published: August 2019

<p>Penyakit pencernaan ataupun penyakit lambung adalah penyakit yang sering dialami oleh masyarakat. Kesadaran akan kesehatan masyarakat yang masih rendah, kebiasaan hidup yang selalu ingin hidup praktis, pengetahuan masyarakat yang sedikit dari gejala awal dari suatu penyakit merupakan faktor-faktor penyebab penyakit menjadi parah ketika penderita ditangani oleh tenaga paramedis. Penelitian ini bertujuan untuk membuat sebuah aplikasi sistem pakar diagnosa penyakit lambung berbasis web. Penelitian ini mempunyai tiga tahap yaitu pertama tahap pengumpulan data dan wawancara dengan tiga dokter yang ada dipuskesmas kecamatan Grogol Petamburan. Tahap kedua adalh membuat rule sebanyak 6 rule dan menentukan gejala sebanyak 36 gejala dan tahap terakhir adalah Implementasi Sistem Pakar berbasis Web. Metode yang digunakan adalah Metode Forward Chaining dimana metode ini mempunyai cara kerja dengan memulai proses pencarian dari sekumpulan data atau fakta, dari data-data tersebut menghasilkan suatu kesimpulan.</p>
LAS MATEMATIKA BERBASIS AKTIVITAS KREATIF DALAM PENINGKATAN AKTIVITAS BELAJAR SISWA
Authors: Suriyani Suriyani , Pasaribu Laili Habibah 
Published: August 2019

<p>Fokus penelitian ini adalah mengembangkan instrumen pembelajaran berupa LAS berbasis aktivitas kreatif untuk digunakan dalam pembelajaran di kelas dan menganalisis hubungan penggunaan LAS tersebut pada peningktan aktivitas kreatif siswa. Analisis validasi soal dalam kategori valid dengan indeks thitung&gt;tTabel yaitu pada masing-masing item soal (1a) 5,18 &gt; 2,0; (1b) 3,88 &gt; 2,04; (2a) 2,84 &gt; 2,04; (2b) 5,18 &gt; 2,04; (3) 5,05 &gt; 2,04. Dari aspek realibilitas diperoleh indeks r11 = 0,53 yaitu kategori sedang; daya beda soal memiliki indeks rata-rata 0,365 yaitu baik, dan tingkat kesukaran soal memiliki indeks yang beragam sehingga soal yang disajikan sudah cukup baik dan layak untuk digunakan dalam pembelajaran di sekolah yang ingin mengedepankan aktifitas kreatif siswa. Berdasarkan hasil analisis perbedaan rata-rata antara kemampuan berpikir kretif siswa sebelum dan sesudah menggunakan LAS berbasis kreatif diperoleh hasil bahwa peningkatan aktivitas belajar siswa meningkat signifikan dengan melihat indeks Gain sebelum dan sesudah menggunakan LAS sebesar 3,157.</p>
KARAKTERISTIK PERMUKIMAN DAERAH KOTO DI KABUPATEN KUANTAN SINGINGI, PROVINSI RIAU
Authors: Yandri Sepli , Sari Suzanna Ratih , Sardjono Agung Budi 
Published: August 2019

Abstract: An area usually has an important area as the center of government activities. as found in every area in Kuantan Singingi Regency. Every region in Kuantan Singingi district has an area that functions as a customary area. the customary area is named as koto. Traditional Koto settlements are one of the traditional settlements in Kuantan Singingi that try to maintain culture amid the development of modernization. In the Koto area, there are buildings that function as customary buildings, namely in the form of the Koto mosque, balai, and Koto house building. These three buildings are a sign that this area is the center of traditional activities in a region. The purpose of this study was to find buildings that functioned as traditional activities and concluded the characteristics of settlements which became the identity of traditional settlements in Kuantan Singingi. The method of descriptive analysis is used as a method of delivering descriptions relating to the traditional settlement of the Koto area by analyzing traditional settlements in the Koto Benai and Koto Sentajo.Keyword: Traditional Settlement, Vernacular Architecture, Kuantan Singingi Traditional House, Koto House.Abstract: Suatu daerah memiliki sebuah kawasan yang penting sebagai pusat kegiatan pemerintahan. Begitu pula yang terdapat pada setiap kenegrian di Kabupaten Kuantan Singingi. Setiap kenegrian di Kuantan Singingi memiliki daerah yang berfungsi sebagai kawasan adat. Kawasan adat tersebut diberi nama dengan sebutan koto. Permukiman tradisional koto menjadi salah satu permukiman tradisional di Kuantan Singingi yang berusaha mempertahankan budaya ditengah perkembangan modernisasi. Pada daerah koto terdapat bangunan yang berfungsi sebagai bangunan adat yaitu berupa bangunan mesjid, bangunan balai adat, dan bangunan rumah adat. Ketiga bangunan ini menjadi pertanda bahwa daerah ini adalah sebagai pusat kegiatan adat istiadat dalam suatu nagori (negri). Tujuan penelitian ini untuk menemukan bangunan yang berfungsi sebagai aktivitas adat dan menyimpulkan karakteristik permukiman yang menjadi identitas permukiman tradisional di Kuantan Singingi. Metode deskripsi analisis, di pakai sebagai cara penyampaian deskripsi mendalam terkait permukiman adat daerah koto dengan menganalisa permukiman tradisional pada daerah Koto Benai dan Koto Sentajo.Kata Kunci : Permukiman Tradisional, Arsitektur Vernakular, Rumah Adat Kuantan Singingi, Rumah koto.
PENGARUH MOTIVASI DAN KEBIASAAN BELAJAR SISWA TERHADAP HASIL BELAJAR MATEMATIKA
Authors: Rosyadi  
Published: August 2019

This research aims to determine: (1) a positive influence of student’s motivation towards their mathematics learning outcomes; (2) a positive influence of student’s learning habits towards mathematics learning outcomes; (3) and a positive influence of the student’s between motivation and study habits combined toward mathematics learning outcomes. The research was conducted using a survey method explanation. In this research, there are three instruments. The instrument for the student’s mathematics learning outcomes is in essay form. While the instrument for student's motivation and study habits are in the form of questionnaires. Data analysis technique used in this research is simple regression and double regression. Based on the analysis, it can be concluded that: (1) there is a positive influence of the student's motivation towards their mathematics learning outcomes; (2) there is a positive influence of the student's study habits towards their learning outcomes; and (3) there is a positive influence motivation and study habits of students combined toward their outcomes in learning mathematics.
PEMBELAJARAN STATISTIKA MELALUI PROJECT BASED LEARNING DENGAN BANTUAN MICROSOFT EXCEL UNTUK MENINGKATKAN HASIL BELAJAR PESERTA DIDIK VIII D SMPN UNGGULAN SINDANG INDRAMAYU
Authors: Herawati Eti 
Published: August 2019

The aim of this research is to improve the learning outcomes of VIIID students of SMP Negeri Unggulan Sindang Indramayu school year 2014/2015 on Statistic material through Project Based Learning Model-Aided Microsoft Excel. The subjects of this research is VIII D students of SMP Negeri Unggulan Sindang Indramayu school year 2014/2015, consist of 30 students, 19 girls and 11 boys. Data collection techniques in this study using observation sheets, tests, observations, questionnaires, interviews and documentation. Statistics attitude towards learning through Project Based Learning Model-Aided Microsoft Excel mostly been good and very good. Can be seen the percentage of successful the first cycle increased from 86.67% to 100% in the second cycle. Percentage mastery learning outcomes in mathematics learning competency skills is increase. The percentage of successful at first cycle increased from 73% to 86.67% in the second cycle. Learning outcomes based on knowledge an increasing number of which reached KKM cycle I to cycle II. KKM percentage reached 76.67% in the first cycle increased to 86.67% in the second cycle. Based on the research that has been conducted, statistics learning using Project Based Learning Model-Aided Microsoft Excel is able to improve the learning outcomes of class VIIID students of SMP Negeri Unggulan Sindang Indramayu school year 2014/2015. The application of statistical learning using Project Based Learning Model-Aided Microsoft Excel as an alternative model of learning that can be applied to topics and other subjects but required good planning and proper time management.