bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neoadjuvant cisplatin and paclitaxel modulate tumor-infiltrating T cells in patients with cervical cancer
Authors: Heeren A. Marijne , van Luijk Iske F. , Lakeman Joost , Pocorni Noëlle , Kole Jeroen , de Menezes Renée X. , Kenter Gemma G. , Bosse Tjalling , de Kroon Cornelis D. , Jordanova Ekaterina S. 
Published: October 2019

Abstract
Resistance to chemotherapy is widely recognized as one of the major factors limiting therapeutic efficacy and influences clinical outcomes in patients with cancer. Many studies on various tumor types have focused on combining standard-of-care chemotherapy with immunotherapy. However, for cervical cancer, the role of neoadjuvant chemotherapy (NACT) on the local immune microenvironment is largely unexplored. We performed a pilot study on 13 primary cervical tumor samples, before and after NACT, to phenotype and enumerate tumor-infiltrating T-cell subpopulations using multiplex immunohistochemistry (CD3, CD8, FoxP3, Ki67, and Tbet) and automated co-expression analysis software. A significant decrease in proliferating (Ki67+) CD3+CD8− T cells and FoxP3+(CD3+CD8−) regulatory T cells was observed in the tumor stroma after cisplatin and paclitaxel treatment, with increased rates of cytotoxic CD8+ T cells, including activated and CD8+Tbet+ T cells. No effect was observed on the number of tumor-infiltrating T cells in the cervical tumor microenvironment after treatment with cisplatin only. Therefore, we conclude that patients treated with cisplatin and paclitaxel had more tumor-infiltrating T-cell modulation than patients treated with cisplatin monotherapy. These findings enhance our understanding of the immune-modulating effect of chemotherapy and warrant future combination of the standard-of-care therapy with immunotherapy to improve clinical outcome in patients with cervical cancer.
Preparation and characterization of electrodeposited Ni-Ru alloys: morphological and catalytic study
Authors: Kutyła Dawid , Kołczyk-Siedlecka Karolina , Kwiecińska Anna , Skibińska Katarzyna , Kowalik Remigiusz , Żabiński Piotr 
Published: October 2019

Abstract
Nickel-ruthenium alloys with various compositions have been deposited by electrodeposition for the first time. Cyclic voltammetry and linear stripping voltammetry measurements show that codeposition of nickel with ruthenium is possible below the potential value of nickel reduction. High-quality alloys containing nickel and ruthenium can be plated at cathodic potentials ranging from − 0.5 to − 1.0 V vs SCE. Deposited coatings were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), and atomic force microscopy (AFM). The diffractograms obtained show that an increase of nickel concentration in alloy will lead to a change in the phase composition and formation of NiRu (100) and (101) phases which is observed to be 78 mas.% Ni. SEM studies confirm the surface homogeneity and presence of small, regular grains. AFM observation allows the estimation of the real surface area of obtained alloys which increase with more negative electrodeposition potentials. Ni-Ru alloys were found to be highly electroactive in the water splitting process, which can be connected with the presence of the NiRu phase and a well-developed electroactive area.
Intermittent application of external positive pressure helps to preserve organ viability during ex vivo perfusion and culture
Authors: Sano Kazunori , Homma Jun , Sekine Hidekazu , Kobayashi Eiji , Shimizu Tatsuya 
Published: October 2019

Abstract
The perfusion of medium through blood vessels allows the preservation of donor organs and culture of bioengineered organs. However, tissue damage due to inadequate perfusion remains a problem. We evaluated whether intermittent external pressurization would improve the perfusion and viability of organs in culture. A bioreactor system was used to perfuse and culture rat small intestine and femoral muscle preparations. Intermittent positive external pressure (10 mmHg) was applied for 20 s at intervals of 20 s. Intermittent pressurization resulted in uniform perfusion of small intestine preparations and minimal tissue damage after 20 h of perfusion, whereas non-pressurized (control) preparations exhibited significantly worse perfusion of the upper surface than the lower surface and histologic evidence of tissue damage. Longer term studies were undertaken in luciferase-expressing rat femoral muscle preparations. Compared with non-pressurized controls, intermittent pressurization led to better perfusion throughout the 14-day experimental period, improved organ viability as indicated by a higher bioluminescence intensity after perfusion with luciferin, and reduced levels of tissue necrosis with better preservation of vascular structures and skeletal muscle nuclei (histologic analyses). Therefore, intermittent application of external positive pressure improved the perfusion of small intestine and skeletal muscle preparations and enhanced tissue viability when compared with controls. We anticipate that this innovative perfusion technique could be used to improve the preservation of donor organs and culture of bioengineered organs.
Ecotropic viral integration site 1 regulates EGFR transcription in glioblastoma cells
Authors: Mizuguchi Asako , Yamashita Shinji , Yokogami Kiyotaka , Morishita Kazuhiro , Takeshima Hideo 
Published: October 2019

AbstractPurposeEcotropic viral integration site-1 (EVI1) is a transcription factor that contributes to the unfavorable prognosis of leukemia, some epithelial cancers, and glial tumors. However, the biological function of EVI1 in glioblastoma multiforme (GBM) remains unclear. Based on microarray experiments, EVI1 has been reported to regulate epidermal growth factor receptor (EGFR) transcription. Signal transduction via EGFR plays an essential role in glioblastoma. Therefore, we performed this study to clarify the importance ofEVI1in GBM by focusing on the regulatory mechanism between EVI1 andEGFRtranscription.MethodsWe performed immunohistochemical staining and analyzed the EVI1-expression in glioma tissue. To determine the relationship betweenEVI1andEGFR, we induced siRNA-mediated knockdown ofEVI1in GBM cell lines. To investigate the region that was essential for the EVI1 regulation ofEGFRexpression, we conducted promoter reporter assays. We performed WST-8 assay to investigate whether EVI1 affected on the proliferation of GBM cells or not.ResultsIt was observed that 22% of GBM tissues had over 33% of tumor cells expressing EVI1, whereas no lower-grade glioma tissue had over 33% by immunohistochemistry. In A172 and YKG1 cells, the expression levels of EGFR and EVI1 correlated. Analysis of theEGFRpromoter region revealed that the EGFR promoter (from − 377 to − 266 bp) was essential for the EVI regulation ofEGFRexpression. We showed that EVI1 influenced the proliferation of A172 and YKG1 cells.ConclusionThis is the first study reporting the regulation ofEGFRtranscription by EVI1 in GBM cells.
Health-related quality of life among Syrian refugees resettled in Sweden
Authors: Gottvall Maria , Sjölund Sara , Arwidson Charlotta , Saboonchi Fredrik 
Published: October 2019

Abstract

Purpose
The main purpose of this study was to assess health-related quality of life (HRQoL) among Syrian refugees resettled in Sweden. Further, we wanted to investigate whether sex, age, education, area of residence, cohabitation and social support were associated with HRQoL in this population.

Methods
This is a cross-sectional study including 1215 Syrian refugees from a randomly selected sample frame resettled in Sweden between the years 2011 and 2013. HRQoL was measured by the EQ-5D-5L descriptive system, and EQ-5D-5L index values were calculated. Associations between sex, age, education, area of residence, cohabitation, social support and EQ-5D-5L were investigated using multiple linear regression analysis.

Results
Depression/anxiety was the most commonly (61.9%) reported EQ-5D-5L problem among the group of Syrian refugees. The mean EQ-5D-5L index value was found to be 0.754. Male sex, younger age, cohabitation and social support were found associated with a higher EQ-5D-5L index score.

Conclusions
Our results concerning long-lasting health problems among the study population indicate that there is a profound need for policies and interventions promoting refugees’ health. Our results also show that social support, a modifiable factor, is relevant to refugees’ overall health, pointing to the importance of public health interventions and policies targeting the facilitation, mobilization and enhancing of refugees’ social support.
Post Materialism and Comparative Economic Development: Do Institutions Act as Transmission Channel?
Authors: Jordaan Jacob A. , Dima Bogdan 
Published: October 2019

Abstract
Although a growing number of countries are experiencing structural societal changes characterised by an increased prominence of post materialistic societal goals and personal values, there is limited empirical evidence on the economic effects of post materialism. In this paper, we address this gap in the literature by estimating the effect of post materialism on the level of economic development for a heterogeneous set of countries. Furthermore, in line with the notion that institutions may act as transmission channel of economic effects of social values, we estimate whether institutions transmit indirect effects from post materialism. Our findings show that post materialism and institutions both generate sizeable effects on economic development. Institutions create consistent positive effects. The effect of post materialism consists of a negative direct effect and a larger positive indirect effect transmitted via institutions related to personal freedom, a solid regulatory framework and low corruption. These findings reflect the importance of accounting for the effects of social values such as post materialism and institutions as well as their interrelationship to identify more fully their impact on economic development.
Cardiac Protection after Systemic Transplant of Dystrophin Expressing Chimeric (DEC) Cells to the mdx Mouse Model of Duchenne Muscular Dystrophy
Authors: Siemionow Maria , Malik M. , Langa P. , Cwykiel J. , Brodowska S. , Heydemann A. 
Published: October 2019

AbstractDuchenne Muscular Dystrophy (DMD) is a progressive lethal disease caused by X-linked mutations of the dystrophin gene. Dystrophin deficiency clinically manifests as skeletal and cardiac muscle weakness, leading to muscle wasting and premature death due to cardiac and respiratory failure. Currently, no cure exists. Since heart disease is becoming a leading cause of death in DMD patients, there is an urgent need to develop new more effective therapeutic strategies for protection and improvement of cardiac function. We previously reported functional improvements correlating with dystrophin restoration following transplantation of Dystrophin Expressing Chimeric Cells (DEC) of myoblast origin in themdxandmdx/scidmouse models. Here, we confirm positive effect of DEC of myoblast (MBwt/MBmdx) and mesenchymal stem cells (MBwt/MSCmdx) origin on protection of cardiac function after systemic DEC transplant. Therapeutic effect of DEC transplant (0.5 × 106) was assessed by echocardiography at 30 and 90 days after systemic-intraosseous injection to themdxmice. At 90 days post-transplant, dystrophin expression in cardiac muscles of DEC injected mice significantly increased (15.73% ± 5.70 –MBwt/MBmdxand 5.22% ± 1.10 – MBwt/MSCmdxDEC) when compared to vehicle injected controls (2.01% ± 1.36) and, correlated with improved ejection fraction and fractional shortening on echocardiography. DEC lines of MB and MSC origin introduce a new promising approach based on the combined effects of normal myoblasts with dystrophin delivery capacities and MSC with immunomodulatory properties. Our study confirms feasibility and efficacy of DEC therapy on cardiac function and represents a novel therapeutic strategy for cardiac protection and muscle regeneration in DMD.
Adjuvant breast cancer: (neo)adjuvant therapy for HER2-positive breast cancer
Authors: Suppan Christoph , Balic Marija 
Published: October 2019

Summary
Early stage HER2-positive cancer outcomes have been substantially improved over the last two decades, but there is still some potential for improvement. Several studies on the preoperative/postoperative treatment of HER2-positive breast cancer were presented at the American Society of Clinical Oncology (ASCO) 2019 annual meeting.
Assessment of the Addition of Fluorapatite–Alumina Coating for a Durable Adhesion of the Interface Prosthesis/Bone Cells: Implementation In Vivo
Authors: Ghorbel Halima , Guidara Awatef , Guidara Racem , Trigui Moez , Bouaziz Jamel , Keskes Hassib , Coddet Christian 
Published: October 2019

Abstract

Purpose
Fluorapatite (Fap), an alumina (Al2O3) known for its excellent bio-inertia, can significantly increase the biocompatibility and bioactivity of biomaterials. This study is an investigation of the interface bone/Al2O3–Fap composite coatings implanted in the tibia of the rabbit.

Methods
Two techniques T1 and T2 were implemented on four rabbits, R1–R4 to assess the effect of the alumina (Al2O3)–Fap [Ca10(PO4)6F2] composite, coating for 316L stainless steel, on bone contact. The fluorapatite powder was synthesized using a wet-chemical method. The biocompatibility and the bioactivity of the Al2O3–Fap composite were evaluated by in vitro/in vivo tests. The characteristics of the bone/implant interface were investigated with scanning electron microscopy, radiology and 3D scanner.

Results
The results showed that T2 was more effective than T1, with a good contact between the implant/bone on the radiographs performed after 28 days. Implants coated with alumina did not show any integration signs with bone tissue. The addition of Fap to alumina coating would increase the adhesion of prosthesis on bone cells and guarantee a stable implantation.

Conclusion
Al2O3–Fap coating showed excellent behavior in vitro and in vivo tests revealing that the Fap is effective in improving biocompatibility and bioactivity.
Ten-year survival outcomes for patients with early stage classical Hodgkin lymphoma
Authors: Myint Zin W. , Shrestha Runa , Siddiqui Salahuddin , Slone Stacey , Huang Bin , Ramlal Reshma , Monohan Gregory P. , Hildebrandt Gerhard C. , Saeed Hayder 
Published: October 2019

Objective/Background:
Early stage classical Hodgkin lymphoma (cHL) has an excellent outcome. Recent studies focus on decreasing toxicity related to the addition of radiation along with chemotherapy. Real-life reporting of the addition of radiation to chemotherapy is lacking. This study investigates the outcomes obtained from a statewide cancer registry for early stage cHL patients treated with chemotherapy alone (CT) versus patients treated with the combined modality of chemotherapy and radiation (CMT).

Methods:
A retrospective study of cHL patients diagnosed and treated was identified using a statewide cancer registry from 2005 to 2014. Patients with early stage disease (I, II) were then grouped on the basis of the presence of B symptoms into favorable and unfavorable groups. Baseline characteristics (age, gender, extranodal involvement, and histology) as well as overall survival were compared for both groups depending on whether they received CT or CMT as first line therapy for their cHL.

Results:
A total of 961 patients were identified; of those, 127 were excluded as they received only radiation or another form of treatment. Of the remaining patients, 293 were categorized as early stage favorable cHL (Group 1) and 130 adults were in the unfavorable cHL (Group 2). There were 335 patients with advanced stage cHL (Group 3) and 76 patients in an unknown stage. The 10-year overall survival for Group 1 was 81.3% versus 76.3% for Group 2 and 52.7% for Group 3. For Group 1, 10-year overall survival was 86.7% with CMT versus 75.1% for those receiving CT only (p = .004). For Group 2, there was no difference in 10-year overall survival between the CMT group (80.0%) and CT (72.5%) (p = .73).

Conclusion:
While radiation therapy might increase long-term toxicity in cHL, in our large data cohort, radiotherapy consolidation as part of the initial therapy for early stage disease provides superior survival at 10 years, especially in favorable risk cHL.
Energy budget in internal wave attractor experiments
Authors: Davis Géraldine , Dauxois Thierry , Jamin Timothée , Joubaud Sylvain 
Published: October 2019

The current paper presents an experimental study of the energy budget of a two-dimensional internal wave attractor in a trapezoidal domain filled with uniformly stratified fluid. The injected energy flux and the dissipation rate are simultaneously measured from a two-dimensional, two-component, experimental velocity field. The pressure perturbation field needed to quantify the injected energy is determined from the linear inviscid theory. The dissipation rate in the bulk of the domain is directly computed from the measurements, while the energy sink occurring in the boundary layers is estimated using the theoretical expression for the velocity field in the boundary layers, derived recently by Beckebanze et al. (J. Fluid Mech., vol. 841, 2018, pp. 614–635). In the linear regime, we show that the energy budget is closed, in the steady state and also in the transient regime, by taking into account the bulk dissipation and, more importantly, the dissipation in the boundary layers, without any adjustable parameters. The dependence of the different sources on the thickness of the experimental set-up is also discussed. In the nonlinear regime, the analysis is extended by estimating the dissipation due to the secondary waves generated by triadic resonant instabilities, showing the importance of the energy transfer from large scales to small scales. The method tested here on internal wave attractors can be generalized straightforwardly to any quasi-two-dimensional stratified flow.
Effect of Serpentinite Dehydration in Subducting Slabs on Isotopic Diversity in Recycled Oceanic Crust and Its Role in Isotopic Heterogeneity of the Mantle
Authors: Shimoda G. , Kogiso T. 
Published: October 2019

AbstractWe conducted geochemical modeling of the isotopic evolution of subducted oceanic crust that takes into account the chemical variation produced at mid‐ocean ridges and in subduction zones, and examined the suitability of our model for generating the high‐μ (HIMU), focal zone (FOZO), and prevalent mantle (PREMA) mantle components. Chemical variation produced at mid‐ocean ridges was represented by the chemical compositions of two groups of mid‐ocean ridge basalts (depleted and enriched). Chemical variation produced in subduction zones was investigated with pressure‐temperature paths of slabs of different ages (governing the physicochemical conditions of element exchanges), as determined using representative subduction zones involving young (hot), intermediate, and old (cold) slabs. The results suggest that dehydration of oceanic crust cannot alone produce isotopic variation beyond the bounds of PREMA compositions. Producing the wider range of isotopic diversity from PREMA to FOZO requires various degrees of element partitioning between subducted oceanic crust and fluids (aqueous or supercritical) released by dehydration of slab serpentinite. The extremely radiogenic Pb isotopic signature of HIMU can only be produced by extensive reaction between subducted oceanic crust and fluids derived from slab serpentinite along the specific geothermal gradient resulting from the relatively slow descent of moderately old slabs. The rarity of such tectonic conditions explains the scarcity of HIMU.
Investigating the Role of the Relative Humidity in the Co‐Occurrence of Temperature and Heat Stress Extremes in CMIP5 Projections
Authors: Brouillet Audrey , Joussaume Sylvie 
Published: October 2019

AbstractHeat stress is expected to intensify, since temperatures are projected to increase during the 21st century. We investigate the assumed co‐occurrence of annual extremes of temperature and one heat stress metric and assess the effect of relative humidity variations on the heat stress changes. We show in CMIP5 simulations that both extremes tend to co‐occur in Europe and northern America, when the conditions are the hottest and the driest. However, extreme seasons occur later for heat stress than temperature by up to 2 months within the tropics. By 2100, models project a drying that hampers the increase in heat stress extremes. Within northeastern Africa, the slight projected wettening enhances the warming effect on the heat stress changes and induces the maximum heat stress intensification. Models are generally able to represent the phasing of both extremes compared to observations, with large uncertainties over regions experiencing the greatest future heat stress intensification.
The Influence of Volcanic Aerosol Meridional Structure on Monsoon Responses over the Last Millennium
Authors: Fasullo John T. , Otto‐Bliesner Bette L. , Stevenson Samantha 
Published: October 2019

AbstractMonsoon responses to eruptions over the last millennium (LM) are examined in an ensemble of climate simulations as a function of eruption hemisphere. A composite analysis reveals a particularly strong sensitivity of monsoon rainfall in the year following Northern Hemisphere (NH) extratropical eruptions. Additional analysis focusing on the 18th century eruption of Mt. Laki and idealized simulations representing an analogue Southern Hemisphere eruption (SH‐Laki) reveal monsoon responses that are approximately symmetric across hemispheres, despite exhibiting asymmetries in other aspects of the climate response. We conclude that 1) latitudinally mirrored eruptions result in approximately symmetric monsoon responses, 2) disproportionate weakening (strengthening) of NH (SH) monsoons by NH eruptions over the LM resulted in part from their relatively high latitudes, and 3) uncertainty in eruption latitude fundamentally limits our ability to accurately simulate associated monsoon and tropical precipitation responses in nature.
On‐the‐Fly Calculation of Absorbed and Equivalent Atmospheric Radiation Dose in A Water Phantom with the Atmospheric Radiation Interaction Simulator (AtRIS)
Authors: Banjac S. , Heber B. , Herbst K. , Berger L. , Burmeister S. 
Published: October 2019

AbstractComputation of the ionization and radiation dose in arbitrary (exo‐) planetary atmospheres due to energetic particles is recently becoming more important due to several reasons that are either correlated with the detection of trace gases for life on exoplanets or with computing dose rates at arbitrary altitudes in the Earth atmosphere. We previously presented Atmospheric Radiation Interaction Simulator, a new Geant4‐based code tailored specifically to enable parametric studies of radiation propagation through exoplanetary atmospheres (Banjac et al., 2019 https://doi.org/10.1029/2018JA026042). Therein, the calculation of ion‐electron pair production rates, which are a mandatory input for chemical and atmospheric modeling, has been presented and validated against Earth measurements and also other, similar, but solar‐system‐specific Geant4‐based codes (PLANETOCOSMICS). In addition to providing input for atmospheric modeling of exoplanets, with AtRIS we aim to directly characterize the habitability by calculating the absorbed dose. In this technical validation study, after showing a detailed analysis of the secondary particles contributing to the atmospheric radiation, we describe a feature of the code which makes direct parametric studies of the interrelation of incident radiation and the resulting absorbed dose throughout the atmosphere possible. In a validation case study configured using an atmospheric model obtained with NRLMSISE‐00 and a primary proton and helium GCR flux calculated using a recent improvement of the force‐field approach, we have compared simulation results with measurements obtained with the Flight Radiation Environment Detector (FRED). We show that Atmospheric Radiation Interaction Simulator (AtRIS) can reproduce the measured dose rate dependence on altitude.