bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long noncoding RNA KCNQ1OT1 promotes apoptosis in neuroblastoma cells by regulating miR‐296‐5p/Bax axis
Authors: Li Meng‐Meng , Liu Xiao‐Hui , Zhao Yi‐Chen , Ma Xiao‐Ye , Zhou Yu‐Chen , Zhao Yan‐Xin , Liu Xue‐Yuan 
Published: August 2019

Long noncoding RNAs (lncRNAs) are emerging as important regulators of multiple cellular processes such as cell invasion, growth, apoptosis and differentiation. LncRNAs can function as competing endogenous RNAs (ceRNAs) which sponge and sequester microRNA (miRNA) to regulate specific targets. Previously, we found that the target genes of several miRNAs, including FADD, Fas, Casp and Bax, are related to neuronal apoptosis and form a regulatory network. Among several factors, microRNA‐296‐5p expression was found to be negatively correlated with caspase activity and apoptosis. Here, we aimed to investigate the role of miR‐296‐5p in neuroblastoma (NB) cells. By performing quantitative real‐time PCR (qRT‐PCR), western blot and flow cytometry assays we analysed the expression of apoptotic markers in NB cells transfected with miR‐296‐5p mimics or inhibitor. Pathway‐specific PCR array allowed us to identify the target genes of miR‐296‐5p. Using LncBase online tool, we predicted lncRNA KCNQ1 opposite strand/antisense transcript 1 (KCNQ1OT1) as an upstream regulator of miR‐296‐5p. The binding of KCNQ1OT1 and miR‐296‐5p was validated via RNA immunoprecipitation and Biotin pull‐down assays. We also demonstrate that miR‐296‐5p suppresses apoptosis of NB cells in vitro and in vivo. Mechanistically, miR‐296‐5p directly bound the 3′UTR of Bax mRNA, thus repressing Bax at the mRNA and protein level. Moreover, through bioinformatic analysis and molecular experiments, we showed that KCNQ1OT1 sponged miR‐296‐5p and impaired its effect on NB cell apoptosis. In summary, KCNQ1OT1 is a potent promoting factor of cell apoptosis, which acts by sponging miR‐296‐5p and upregulating Bax. Our findings identify a regulatory axis of cell fate in NB cells.
Biomineralization plasticity and environmental heterogeneity predict geographical resilience patterns of foundation species to future change
Authors: Telesca Luca , Peck Lloyd S. , Sanders Trystan , Thyrring Jakob , Sejr Mikael K. , Harper Elizabeth M. 
Published: August 2019

AbstractAlthough geographical patterns of species' sensitivity to environmental changes are defined by interacting multiple stressors, little is known about compensatory processes shaping regional differences in organismal vulnerability. Here, we examine large‐scale spatial variations in biomineralization under heterogeneous environmental gradients of temperature, salinity and food availability across a 30° latitudinal range (3,334 km), to test whether plasticity in calcareous shell production and composition, from juveniles to large adults, mediates geographical patterns of resilience to climate change in critical foundation species, the mussels Mytilus edulis and M. trossulus. We find shell calcification decreased towards high latitude, with mussels producing thinner shells with a higher organic content in polar than temperate regions. Salinity was the best predictor of within‐region differences in mussel shell deposition, mineral and organic composition. In polar, subpolar, and Baltic low‐salinity environments, mussels produced thin shells with a thicker external organic layer (periostracum), and an increased proportion of calcite (prismatic layer, as opposed to aragonite) and organic matrix, providing potentially higher resistance against dissolution in more corrosive waters. Conversely, in temperate, higher salinity regimes, thicker, more calcified shells with a higher aragonite (nacreous layer) proportion were deposited, which suggests enhanced protection under increased predation pressure. Interacting effects of salinity and food availability on mussel shell composition predict the deposition of a thicker periostracum and organic‐enriched prismatic layer under forecasted future environmental conditions, suggesting a capacity for increased protection of high‐latitude populations from ocean acidification. These findings support biomineralization plasticity as a potentially advantageous compensatory mechanism conferring Mytilus species a protective capacity for quantitative and qualitative trade‐offs in shell deposition as a response to regional alterations of abiotic and biotic conditions in future environments. Our work illustrates that compensatory mechanisms, driving plastic responses to the spatial structure of multiple stressors, can define geographical patterns of unanticipated species resilience to global environmental change.
Not Bound but Committed: Operationalizing the Global Compact on Refugees
Authors: Gilbert Geoff 
Published: August 2019

AbstractThe Global Compact on Refugees is not legally binding, but it gives rise to commitments by the international community as a whole. It is also rooted in international refugee law, international human rights law and international humanitarian law. This article addresses how the GCR cannot give rise to binding obligations in international law, yet provide for enhanced protection and assistance to refugees and hosting communities, and establish commitments for a fairer and more predictable sharing of burdens and responsibilities. It does this by reference to other non‐legally binding international documents and rules of law. Additionally, the use of indicators to measure states’ and other international actors’ performance in operationalizing the GCR provides a framework to measure commitments; coupled with greater humanitarian and development co‐operation, commitments can be better facilitated even if the GCR is not legally binding. Finally, the sharing of burdens and responsibilities is also fulfilled by the emphasis on solutions.
Synthesis and optical properties of novel mixed‐metal cation CsPb1−xTixBr3‐based perovskite glasses for W‐LED
Authors: Shen Linli , Zhang Zelong , Zhao Yi , Yang Haisheng , Yuan Lin , Chen Ya , Xiang Weidong , Liang Xiaojuan 
Published: August 2019

AbstractIn this report, a mixed‐metal cation‐based halide perovskite (HP) CsPb1−xTixBr3 quantum dots (QDs) were first embedded in the B–Si–Zn glasses using a traditional approach of melt quenching and heat treating. A battery of test results such as photoluminescence, X‐ray diffraction, and time‐resolved attenuation prove that Ti ions do not destroy the properties of CsPbBr3, and they are successfully doped into CsPbBr3. At the same time, the doping of Ti ions also reduces the toxicity of lead. By altering the ratio of Pb/Ti, we determined the optimum ratio of CsPb0.7Ti0.3Br3 QDs through experimental data. Due to the excellent optical properties and stability of CsPb0.7Ti0.3Br3 QDs glass, it was designed to construct the white‐light emitting diode device with tunable color coordinate, color rendering index, correlated color temperature, and a high luminous efficiency compared with CsPbBr3 QDs glass, which may be a promising candidate for the field of lighting and displays.
A Participatory Social A/r/tography: Bodies and Houses Metamorphosing from Schools in Tegucigalpa to a Liverpool Tate Exchange Event
Authors: Marin‐Viadel Ricardo , Arias‐Camison Alicia , Varea Ana 
Published: August 2019

AbstractSocial a/r/tography combines three dimensions of a/r/tography (artistic creation, education and research) incorporating a fourth collaborative, participatory community‐based approach. This ongoing project began in two primary and secondary schools located in the slums of Tegucigalpa, Honduras, as cooperative action for curricular development in art education. As participatory action, students created a mural drawing inspired by Sol Lewitt's wall drawings, as well as photo pairs on the theme of body and nature inspired by a lithograph by Uta Barth. The drawings and photographs created by the students in Honduras were presented at an event in the Exchange space of the Tate gallery in Liverpool as visual provocation for the creation of drawings and visual metaphors. The concept of visual dialogue, which we define as ‘to teach human beings to have visual conversations with other humans through the visual content of images’, helps us to consider images as questions which are answered with new images whose meaning is subsequently transformed in function of artistic, school and social contexts.
Arts‐Based Educational Research in Museums: ‘Art for Learning Art’, an A/r/tographic Mediation
Authors: Palau‐Pellicer Paloma , Mena Jaime , Egas Olga 
Published: August 2019

AbstractThis article discusses forms of arts‐based mediation in museums that use creation as the primary tool in the learning process. We present four mediation experiences based on the arts‐based teaching methodologies promoted by the project ‘Art for Learning Art’. These experiences have been developed in four museums: the Centre Pompidou Málaga, the State Russian Museum in Málaga and the Espai d'Art Contemponeo [Center of contemporary art] in Castellón in Spain and the Modern Art Murilo Mendes Museum of Minas Gerais in Brazil. Four experiences in three cities of two countries that work in a methodology which involves participative and collaborative visitor connections with the works exhibited using arts‐based strategies. In all cases, the artistic works of the exhibitions are the conceptual basis for mediation proposals that are offered to the public in order to encourage participation. We link the aesthetic experience as the origin of the mediation process in two fundamental aspects: creation and appreciation. In the act of creative appreciation, most art education objectives are met. The actions and processes are directed by collaborative and contemporary creation strategies around two axes: museum educator‐artists in training and visitors‐artists as learners. In the four experiences that we present here, this confluence takes place. The four events have been adapted to the conditions of the place, public and art exhibitions, contributing new approaches to the model that has been promoted from the University of Granada since 2013.
Cultural Sustainability in Art‐Based Interdisciplinary Dialogue
Authors: Härkönen Elina , Stöckell Antti 
Published: August 2019

AbstractThis article introduces an international and interdisciplinary summer school, ‘Living in the Landscape’ (LiLa) in 2018. LiLa's practices focused on creating dialogue among art education, anthropology and nature science and developing culturally sustainable methods for investigating cultural heritage in the Komi Republic of Russia. The article's research interest is how dialogue and cultural heritage appear in the artistic processes, artworks and final exhibition of the summer school. These are examined through art‐based action research in order to develop international, multidisciplinary and culturally sustainable art education. The four‐field model utilised in the research highlights the multidimensional role of dialogue in both individual and collaborative artistic endeavours.
Efficacy and safety of single vs dual antiplatelet therapy in patients on anticoagulation undergoing percutaneous coronary intervention: A systematic review and meta‐analysis
Authors: Atti Varunsiri , Turagam Mohit K. , Garg Jalaj , Velagapudi Poonam , Patel Nileshkumar J , Basir Mir B , Mujer Mark TP , Rayamajhi Supratik , Abela George S , Koerber Scott , Gopinnathanair Rakesh , Lakkireddy Dhanunjaya 
Published: August 2019

AbstractBackgroundSelection of an appropriate antithrombotic regimen in patients requiring oral anticoagulation (OAC) undergoing percutaneous coronary intervention (PCI) still remains a challenge. An ideal 9–2regimen should balance the risk of bleeding against ischemic benefit.MethodsA comprehensive literature search for studies comparing triple antithrombotic therapy (TAT) vs double antithrombotic therapy (DAT) in patients requiring OAC undergoing PCI was performed in clinicalTrials.gov, PubMed, Web of Science, EBSCO Services, Cochrane Central Register of Controlled Trials, Google Scholar, and various scientific conference sessions from inception to May 1st, 2019. A meta‐analysis was performed using random‐effects model to calculate risk ratio (RR) and 95% confidence interval (CI).ResultsFifteen studies were eligible and included 13 967 patients, of which 7349 received TAT and 6618 received DAT. Compared with DAT, TAT was associated with lower risk of myocardial infarction (RR, 0.82; 95%CI, 0.69–0.98; P = .03) and stent thrombosis (RR, 0.66; 95%CI, 0.46–0.96; P = .03). There was no difference in risk of trial defined major adverse cardiac events, all‐cause mortality, and stroke between two groups. Compared with DAT, TAT was associated with higher risk of trial defined major bleeding (RR, 1.67; 95%CI, 1.38–2.01; P < .00001), including thrombolysis in myocardial infarction major bleeding (RR, 1.81; 95%CI, 1.47–2.24; P < .00001) but no significant difference in risk of intracranial bleeding.ConclusionIn patients requiring OAC undergoing PCI, TAT was associated with a lower risk of myocardial infarction but with a significantly higher risk of major bleeding when compared with DAT.
Ventricular tachycardia in nonischemic cardiomyopathy: Anteroseptal vs inferolateral origin based on ICD ventricular electrogram timing
Authors: Kodali Santhisri , Shirai Yasuhiro , Muser Daniele , Callans David J. , Marchlinski Francis E. , Santangeli Pasquale 
Published: August 2019

AbstractIntroductionThe majority of patients with nonischemic cardiomyopathy (NICM) present a perivalvular substrate that is either predominantly antero‐septal (AS) or infero‐lateral (IL), corresponding to specific ventricular tachycardia (VT) morphologies. The relative timing of far‐field and near‐field ventricular electrograms (EGMs) from stored implantable cardioverter‐defibrillator (ICD) events of VT may be used to distinguish AS from IL VT in NICM.Methods and ResultsWe analyzed 48 patients with NICM with either a primarily AS (54%) or IL (56%) VT source undergoing catheter ablation between 2003 and 2018. Only patients with retrievable ICD‐EGMs of spontaneous VT events which could be matched with VTs induced during the ablation procedure were included. A total of 56 VT events (52% AS origin and 48% IL origin) were analyzed, yielding a mean far‐field to near‐field interval of 31 ± 13 milliseconds for AS VTs and 47 ± 19 milliseconds for IL VTs (P = .001). At receiver operating characteristic analysis (AUC = 0.734), a far‐field to near‐field interval of ≥ 60 milliseconds ruled out AS VTs in 29 (100%) cases and diagnosed IL VTs with a positive predictive value (PPV) of 100% and a negative predictive value (NPV) of 63%. An interval of ≤ 20 milliseconds ruled out IL VTs in 25 (93%) cases and diagnosed AS VTs with a PPV of 83% and NPV of 57%. Significant overlap between the two groups was observed among far‐field to near‐field intervals in between 20 milliseconds and 60 milliseconds.ConclusionsThe relative timing of far‐field and near‐field EGMs from stored clinical ICD events of VT can be helpful to differentiate AS vs IL origin of VT in NICM.
Correlations between arrhythmogenic substrate and noninvasive risk stratification in ischemic heart disease patients modifications by radiofrequency ablation
Authors: Voglimacci‐Stephanopoli Quentin , Rollin Anne , Mondoly Pierre , Monteil Benjamin , Mandel Franck , Galinier Michel , Carrié Didier , Maury Philippe 
Published: August 2019

AbstractIntroductionSeveral noninvasive risk factors for ventricular arrhythmias have been described in postmyocardial infarction (MI) patients, whose relationships with scar characteristics and modifications by ablation are unknown.MethodsTwenty‐two patients with previous MI referred for ventricular tachycardia ablation were prospectively included. ECG, heart rate variability (HRV), signal‐averaged ECG (SA‐ECG), and T wave alternans (TWA) were performed before and after radiofrequency ablation. Scar areas were correlated to preablation parameters. Pre and postablation parameters were furthermore compared.ResultsLeft ventricular ejection fraction and some spectral and time‐domain HRV parameters were significantly correlated to the scar areas.QRS duration was larger after vs before ablation (120 ± 29 vs 105 ± 22 msec, P = .01). No significant modification in time or spectral domain of HRV was observed. There was no significant change in TWA and SA‐ECG before and after ablation. Borderline decreases in quantitative TWA parameters were noted in patients with positive TWA and successful ablation procedure.ConclusionSome noninvasive risk factors were linked to the scar areas, but few were significantly modified after ablation. Larger populations are needed to demonstrate significant differences or correlations.
Evolution of renal function under direct‐acting antivirals treatment for chronic hepatitis C: A real‐world experience
Authors: Tsai Ming‐Chao , Lin Chun‐Yen , Hung Chao‐Hung , Lu Sheng‐Nan , Tung Shui‐Yi , Chien Rong‐Nan , Lin Chih‐Lang , Wang Jing‐Houng , Chien‐Hung Chen , Chang Kuo‐Chin , Hu Tsung‐Hui , Sheen I‐Shyan 
Published: August 2019

AbstractRenal toxicity of direct‐acting antivirals (DAAs) in chronic hepatitis C (CHC) patients has not been well‐characterized. The aim of this study was to assess renal safety of DAAs in an Asian CHC patient cohort. Data from CHC patients (n = 1536) treated with DAAs were used in this retrospective study. Serial estimated glomerular filtration rate (eGFR) at pretreatment (1‐year prior to treatment), baseline, end of treatment (EOT), and 12 weeks after treatment (SVR12) was evaluated. While a significant decrease in eGFR from baseline to EOT (84.8 → 81.8 mL/min/1.73 m2, P < .001) was observed; subsequently, a slight rise at SVR12 (84.3 mL/min/1.73 m2) was also evident. Changes in eGFR after DAA treatment were similar to those seen in PrOD, DCV/ASV and GZP/EBV regimens, except in the SOF‐based regimen wherein eGFR remained unchanged from EOT to SVR12, especially in liver transplant recipients. Multivariate analysis revealed that age >65 years (OR = 1.862, P = .011), baseline eGFR ≥ 60 mL/min/1.73 m2 (OR = 2.684, P = .023), and liver transplant (OR = 3.894, P = .001) were independent risk factors for deteriorating renal function. In conclusion, DAA treatment led to a significant decline in eGFR at EOT but was followed by a slight rise at 12 weeks after treatment. A similar trend was observed with PrOD, DCV/ASV and GZP/EBV, but not in SOF‐based regimens. As age >65 years, baseline eGFR ≥ 60 mL/min/1.73 m2 and liver transplantation are significant risk factors for deterioration in renal function, we strongly advice close monitoring of renal function in these populations.
Mitochondrial genome of Amblyomma javanense: a hard tick parasite of the endangered Malayan pangolin (Manis javanica)
Authors: Duan D.‐Y. , Tang J.‐M. , Chen Z. , Liu G.‐H. , Cheng T.‐Y. 
Published: August 2019

AbstractAmblyomma javanense is an important ectoparasite of Manis javanica, although the population genetics, molecular biology and systematics of A. javanense remain poorly understood. In the present study, the mitochondrial genome of A. javanense was sequenced using the Illumina HiSeq sequencing platform (Illumina, San Diego, CA, U.S.A.) and compared with the genomes of two closely related species: Amblyomma fimbriatum and Amblyomma americanum. The intraspecies and interspecies relationships of A. javanense and another 21 selected species were investigated by constructing a maximum‐likelihood tree and a neighbour‐joining tree. The mitochondrial genome of A. javanense was 14 780 bp in length and contained 13 protein‐coding genes, 22 transfer RNA genes, two ribosomal RNA genes and two control regions. The results of the comparisons indicate that there is great similarity among these three species, and both trees indicate that A. javanense is a member of the Amblyomminae. The study of A. javanense of pangolins also indicates the premise and foundation of the relationship between the parasite and other species.
Olfactory responses of Amblyomma maculatum to rumen fluid and other odourants that attract blood‐seeking arthropods
Authors: Garner K. D. , Payton M. E. , Talley J. L. , Noden B. H. 
Published: August 2019

AbstractAmblyomma maculatum Koch (Ixodida: Ixodidae) has emerged as a significant vector of human and companion animal diseases in the U.S.A. When expanding in range, A. maculatum can be difficult to collect in the field and control on livestock. A novel method is needed to improve the field collection of A. maculatum, as well as to control their effects as ectoparasites of livestock and companion animals. The present study aimed to test the effects of known volatiles on the activation and selection choices of A. maculatum in a laboratory‐based Y‐tube assay and field‐based assays. Although the majority of adult A. maculatum were activated to move by five of the seven semiochemicals tested, only rumen fluid significantly attracted ticks to make a selection in the Y‐tube apparatus. Rumen fluid attracted the most A. maculatum in the laboratory, with 56% (84/150) making it to the rumen Y‐tube arm, although the results were not replicated in semi‐field experiments. These studies highlight the need for continued work to identify attractants for tick vectors that will assist field collections. These attractants could also be incorporated into management strategies that lead to prevention technologies to reduce tick burdens on cattle or in risk areas of humans.
Visual system disorders and musculoskeletal neck complaints: a systematic review and meta‐analysis
Authors: Sánchez‐González María Carmen , Gutiérrez‐Sánchez Estanislao , Sánchez‐González José‐María , Rebollo‐Salas Manuel , Ruiz‐Molinero Carmen , Jiménez‐Rejano José Jesús , Pérez‐Cabezas Verónica 
Published: August 2019

AbstractAccommodation disorders and nonstrabismic binocular dysfunctions affect patients’ binocular system and visual performance. These visual disorders could be associated with musculoskeletal discomfort in the neck and shoulder area. The purpose of this systematic review and meta‐analysis was to ascertain the relationship between visual system disorders and the musculoskeletal system of the neck. The review protocol is available in PROSPERO (CRD42018112771). All articles selected examined the relationship between neck conditions (chronic neck pain and whiplash) and the visual system in adult populations. Studies with optometric or physiotherapeutic measurements were included. Bias risk was evaluated with the modified Cochrane Collaboration Tool and Study Quality Assessment Tool. To provide complete quality assessment evidence, the authors applied the GRADEpro Guideline Development Tool. The literature search was conducted in November 2018 and yielded 745 studies among all the databases. Out of these studies, 21 were finally included. Most of the studies presented a moderate methodological quality. Only one high‐quality trial was found. Based on a qualitative assessment, our systematic review and meta‐analysis revealed that all included studies established a relationship between the visual system and musculoskeletal system of the neck. However, the methods for the measurement of the visual system lacked uniformity.