bluuebunny/crossref_metadata_2025_split huggingface dataset:
PredPVP: A Stacking Model for Predicting Phage Virion Proteins Based on Feature Selection Methods
Authors: Cao Qian , Xiao Xufeng , Bin Yannan , Zhao Jianping , Zheng Chunhou 
Published: October 2024

Background:
Phage therapy has a broad application prospect as a novel therapeutic
method, and Phage Virion Proteins (PVP) can recognize the host and bind to surface receptors,
which is of great significance for the development of antimicrobial drugs for the treatment of infectious
diseases caused by bacteria. In recent years, several PVP predictors based on machine learning
have been developed, which usually use a single feature to train the learner. In contrast, higher dimensional
feature representations tend to contain more potential sequence information.

Methods:
In this work, we construct a stacking model PredPVP for PVP prediction by combining
multiple features and using feature selection methods. Specifically, the sequence is first encoded using
seven features. For this high-dimensional feature representation, three feature selection methods
wereutilized to remove redundant features, then integrated with eight machine learning algorithms.
Finally, probability features and class features (PCFs) generated by 24 base models were put into logistic
regression (LR) to train the model.

Results:
The results of the independent test set indicate that PredPVP has higher performance compared
to other existing predictors, with an AUC of 93.4%.

Conclusion:
We expect PredPVP to be used as a tool for large-scale PVP recognition, providing a
new way for the development of novel antimicrobials and accelerating its application in actual
treatment. The datasets and source codes used in this study are available at
https://github.com/caoqian23/PredPVP.
Proposed Hypothesis of TWEAK/Fn14 Receptor Modulation in Autism Spectrum Disorder
Authors: Khan Heena , Rihal Vivek , Kaur Amarjot , Singh Thakur Gurjeet 
Published: October 2024

Abstract:
Autism Spectrum Disorder (ASD) is a neurodevelopmental disorder with a complex,
multiple etiology that is marked by impaired social interaction, communication, and repetitive behaviour.
There is presently no pharmaceutical treatment for the core symptoms of ASD, even
though the prevalence of ASD is increasing worldwide. Treatment of autism spectrum disorder involves
the interaction of numerous signalling pathways, such as the Wnt/beta-catenin pathway, probiotics
and kynurenine pathway, PPAR pathway, PI3K-AKT-mTOR pathway, Hedgehog signaling
pathway, etc. The scientific literature has revealed TWEAK/Fn14 to not be explored in the autism
spectrum disorder. In vitro and in vivo, TWEAK can control a wide range of cellular responses. Recent
research has revealed that TWEAK and Fn14 are expressed in the Central Nervous System
(CNS) and upregulated in perivascular endothelial cells, astrocytes, neurons, and microglia in response
to various stimuli, including cerebral ischemia. This upregulation is followed by cell death
and an increase in Blood-brain Barrier (BBB) permeability. The study has revealed that Aurintricarboxylic
Acid (ATA) acts as an agent that suppresses TWEAK/Fn14 signaling. Similarly, from the
discussion, it has been emphasized that the proposed molecular TWEAK/Fn14 signalling pathway
can be considered as a therapeutic approach in the management of autism spectrum disorder.
Tanshinone IIA Regulates NRF2/NLRP3 Signal Pathway to Restrain Oxidative Stress and Inflammation in Uric Acid-Induced HK-2 Fibrotic Models
Authors: Zhang Weiliang , Feng Jiashu , Liu Ruiqi , Xiang Ting , Wu Xinlin 
Published: October 2024

Introduction:
This study aims to investigate the function and potential mechanism of
Tanshinone IIA in uric acid-induced HK-2 fibrosis models

Materials and Methods:
An in vitro model of fibrosis was constructed using uric acid stimulation.
RT-qPCR and Western blot were used to evaluate the levels of inflammatory cytokines. The detection
of ROS and ELISA assay were used to analyze the changes in oxidative stress.

Results:
Tanshinone IIA inhibited the increase in inflammatory cytokines TNF-α, IL-1β, IL-6,
and IL-18 and the formation of NLRP3 inflammasome induced by uric acid stimulation. In addition,
Tanshinone IIA treatment reduced the production of ROS and MDA, promoting the expression
of SOD and CAT, thereby protecting HK-2 cells from oxidative stress damage. Besides, the
expression of TGF-β, FN, and COL-1 was significantly reduced by the treatment of Tanshinone
IIA. Mechanistically, Tanshinone IIA inhibited the expression of inflammatory cytokines and the
formation of the NLRP3 inflammasome by targeting NRF2.

Conclusion:
Tanshinone IIA exerts a protective role in uric acid-induced HK-2 fibrosis models by
targeting the NRF2-NLRP3 signaling pathway to reduce the occurrence of inflammation and
oxidative stress.
Flexibilizing Mechanisms of Spinel-, Hercynite-, Galaxite-, and Chromite-Containing Basic Refractories for Cement Rotary Kilns
Authors: Guo Zongqi , Ding Qiang , Ding Haiwei , Dai Yajie , Dang Xiaomei , Gao Jianying , Yuan Wenjie 
Published: October 2024

Background:
Refractory linings of cement rotary kilns are subjected to severe
thermomechanical stresses of cranking, ovality, tyre hooping/migration, and uneven thermal
distribution. An insistent demand is to discover the significance of spinel, hercynite,
galaxite, and chromite in magnesia refractories, as well as their flexibilizing mechanisms.

Objectives:
The objectives are to compare the fracture behavior of magnesia–spinel, –
hercynite, -galaxite, and -chromite refractories and to unveil the flexibilizing mechanisms
of different spinels.

Methods:
The wedge-splitting test is carried out to produce various fracture parameters.
Their flexibilizing mechanisms are unveiled by performing microstructural observations
and analyses.

Results:
Various fracture parameters are obtained, including specific fracture energy, brittleness
number, characteristic crack length, and thermal-shock resistance parameter. Generally,
magnesia–hercynite bricks and magnesia–galaxite bricks have demonstrated the
obvious advantages of fracture resistance, which are more flexible than magnesia–spinel
bricks and magnesia–chromite bricks.

Conclusion:
The flexibility of magnesia–spinel bricks is attributed to microcracks generated
from the thermal mismatch between spinel grains and surrounding periclase, which is
recognized as the thermal-expansion mismatch mechanism. In magnesia–hercynite and
magnesia-galaxite refractories, the active-ion-diffusion mechanism is predominant beyond
similar microcracks, to drive the flexibility by the continuous diffusion of Fe2+, Mn2+, and
Mg2+ during high-temperature processes. In magnesia–chromite bricks, the pore rims contribute
to the flexibility as a silicate-migration mechanism, after silicate envelopes first
arise around chromite grains and then vanish into the surrounding magnesia by the suction
of capillary force during the burning process.
Himalayan Sheep Wool Reinforced Composite- A Novel Sustainable Material for Future
Authors: Rattan Nav , Kaushik Aakanksha J , Kumar Ajay , Phanden Rakesh Kumar , Kumar Ashwini , Goyat Vikas 
Published: October 2024

Background:
Sheep wool-reinforced composites offer a sustainable alternative
with diverse applications. This study explores their properties, focusing on water
absorption behavior and contact angle measurements.

Objective:
To investigate the properties of sheep wool-reinforced composites and evaluate
their suitability for moisture-sensitive environments, with potential for patent protection.

Methods:
Wool fibres, known for their hydrophilic nature, were modified to be hydrophobic
and incorporated into epoxy resin matrices. Different weaving patterns
were utilized to create fibre mats reinforcing epoxy composites.

Results:
2D plane weaving reinforcements exhibited superior in-plane properties
compared to other reinforcements. Utilizing environmental sources like sheep wool in
epoxy composites offers advantages such as low density, cost-effectiveness, and sustainability,
potentially patentable innovations.

Conclusion:
The study demonstrates the developed composites' excellent resistance
to water absorption, making them viable for moisture-sensitive applications. Contact
angle measurements suggest strong interfacial adhesion between wool fibres and the
epoxy matrix, highlighting patent-worthy advancements. These findings underscore the
potential of sheep wool-reinforced composites in sustainable and moisture-resistant applications
across various industries, including automotive, construction, and consumer
goods, emphasizing the importance of patent protection for innovative technologies.
Assessing the Efficacy and Challenges of Tofacitinib in the Management of COVID-19: A Systematic Review and Meta-analysis of Cohort Studies
Authors: Dara Tahereh , Zabihi Mohsen , Hoseinzade Farahnaz , Radandish Mohammad Reza , Saghafi Fatemeh 
Published: October 2024

Objectives:
Global healthcare faces challenges in combating COVID-19, with rising cases
despite widespread vaccination. Severe COVID-19 cases, marked by acute respiratory distress and
cytokine release syndrome, highlight the importance of managing cytokine storms. Janus kinase
(JAK) inhibitors, such as tofacitinib, show promise in this regard. While tofacitinib is recommended
for severe cases, challenges include adverse effects, conflicting studies, and the need for further investigation
of new virus strains. Overcoming these hurdles is crucial for developing an effective
treatment protocol and reducing COVID-19 mortality.

Methods:
This study conducted a comprehensive search across PubMed, Scopus, and ISI Web of
Science for observational studies on tofacitinib treatment in human adults with COVID-19. The
search covered a specified period up to 2024. Data extraction, including study characteristics and
quality assessment, employed the Newcastle Ottawa Scale and a modified Cochrane tool. Statistical
analysis, conducted with Comprehensive Meta-Analysis Software, assessed heterogeneity and significance
levels.

Results:
The meta-analysis of the three studies showed a significant reduction in mortality (Risk Ratio:
0.372, 95% CI: 0.213–0.649, P-value = 0.001) with low heterogeneity (Cochrane P-value =
0.793), while no significant reduction in the need for mechanical ventilation was observed (Cochrane
P-value = 0.194).

Conclusion:
Tofacitinib administration shows a significant reduction in COVID-19 mortality. However,
the limited studies on its efficacy highlight the need for cautious interpretation in clinical assessments.
Evaluation of a Novel Streptomyces avermitilis Strain from Egyptian Soil for Biological Control of Tetranychus urticae in Greenhouse Cucumber Plantations
Authors: Saied Norhan A , Yaseen R. , Mostafa Amal A. , Barghout Marwa E. , Ahmed Rasha H. , Amin Basma H. , Ali M.A. 
Published: October 2024

Background:
Infestation in greenhouse cucumber with the two-spotted spider
mite (Tetranychus urticae Koch) commonly causes severe damage to crop quality and
quantity and increases crop production costs.

Objective:
This study was conducted to investigate the efficacy of high-abamectinproducing
isolates of S. avermitilis against T. urticae-infested cucumber and to assess
their impact on biochemical stress markers in these vegetables.

Methods:
In this study, 72 non-antagonistic Streptomyces were isolated from rhizospheric
soil samples collected from eight different locations in Egypt and screened for their
ability to produce the secondary metabolite, abamectin

Results:
The screening process identified two potent abamectin-producing isolates, EW8
and T2, which produced 42.7 and 29.6 μg/L abamectin, respectively, as confirmed by
LC-MS/MS analysis. According to DNA sequence analysis of the 16S rRNA gene, these
two isolates belong to the species S. avermitilis. The acaricidal activity of either culture
suspensions of S. avermitilis strains WE8 and T2, or their extracts containing abamectin,
against the mobile stages and egg hatchability of T. urticae was evaluated in the laboratory
and the greenhouse. Data on the mortality among the examined female mites and the
reduction in their number of eggs point out a potential acaricidal activity of the examined
strains of S. avermitilis and their extracts containing abamectin against T. urticaes. Furthermore,
the extracts containing abamectin from these two S. avermitilis strains induced
oxidative stress in the infested cucumber plants by T. urticaes, as indicated by increased
levels of malondialdehyde (MDA). However, the levels of MDA in T. urticae-infested
cucumber plants varied depending on the strain and the specific abamectin crude extract
used.

Conclusion:
S. avermitilis strains T2 or WE8, or their crude extract could be applied in
greenhouse cucumber plantations to combat red mite infestation (Fig. 1).
Emotional demonstration (EMO DEMO) to improve exclusive breastfeeding: literature review
Authors: Sembiring Gaipyana , Damayani Ayi Diah , Yunus Endriyani Martina , Pinaremas Annisa Sali 
Published: October 2024

<p><strong><em>Background: </em></strong><em>Suboptimal breastfeeding practices cause 823,000 deaths in children under 5 years of age and 20,000 deaths in women due to breast cancer in the world every year. The Government's program to increase coverage of exclusive breastfeeding is contained in the 10 Steps Towards Successful Breastfeeding (LMKM) which states that health workers are required to provide exclusive breastfeeding education to mothers from the antenatal period. An Emo Demo is a way to convey nutritional messages with a behavior change communication strategy that uses Behavior Centered Design (BCD).</em><strong><em></em></strong></p><p><strong><em>Objectives: </em></strong><em>This literature review aims to analyze the effect of exclusive breastfeeding education using the emotional demonstration method (EMO DEMO) on exclusive breastfeeding.</em><strong><em></em></strong></p><p><strong><em>Methods: </em></strong><em>This research method is a literature review using PRISMA analysis with the PUBMED, Science Direct, Proquest, Springer, and Google Scholar databases from 2019-2023.</em> The inclusion criteria in this Literature Review are using a period from 2019-2023. the type of article is original research. The exclusion criteria in this literature review are research subjects other than pregnant women, mothers who have children under 2 years old, and health cadres.</p><p><strong><em>Results</em></strong><em>: Exclusive breastfeeding education using the emo demo method increases knowledge, attitudes, self-efficacy, and exclusive breastfeeding. Emotional demonstration training increases cadres' knowledge and skills in providing exclusive breastfeeding education.</em></p><p><strong><em>Conclusions:</em></strong><em> </em><em>Exclusive breastfeeding education innovations with emotional demonstration methods will help the government accelerate optimal breastfeeding practices to improve maternal and child health in Indonesia.</em> <em>It is hoped that health workers can apply the emo demo method in providing exclusive breastfeeding education. </em></p><p><em> </em></p>
Snowball Group Usability Testing for Rapid and Iterative Multisite Tool Development: Method Development Study
Authors: Dauber-Decker Katherine L , Feldstein David , Hess Rachel , Mann Devin , Kim Eun Ji , Gautam-Goyal Pranisha , Solomon Jeffrey , Khan Sundas , Malik Fatima , Xu Lynn , Huffman Ainsley , Smith Paul D , Halm Wendy , Yuroff Alice , Richardson Safiya 
Published: October 2024

Abstract

Background
Usability testing is valuable for assessing a new tool or system’s usefulness and ease-of-use. Several established methods of usability testing exist, including think-aloud testing. Although usability testing has been shown to be crucial for successful clinical decision support (CDS) tool development, it is often difficult to conduct across multisite development projects due to its time- and labor-intensiveness, cost, and the skills required to conduct the testing.

Objective
Our objective was to develop a new method of usability testing that would enable efficient acquisition and dissemination of results among multiple sites. We sought to address the existing barriers to successfully completing usability testing during CDS tool development.

Methods
We combined individual think-aloud testing and focus groups into one session and performed sessions serially across 4 sites (snowball group usability testing) to assess the usability of two CDS tools designed for use by nurses in primary and urgent care settings. We recorded each session and took notes in a standardized format. Each site shared feedback from their individual sessions with the other sites in the study so that they could incorporate that feedback into their tools prior to their own testing sessions.

Results
The group testing and snowballing components of our new usability testing method proved to be highly beneficial. We identified 3 main benefits of snowball group usability testing. First, by interviewing several participants in a single session rather than individuals over the course of weeks, each site was able to quickly obtain their usability feedback. Second, combining the individualized think-aloud component with a focus group component in the same session helped study teams to more easily notice similarities in feedback among participants and to discuss and act upon suggestions efficiently. Third, conducting usability testing in series across sites allowed study teams to incorporate feedback based on previous sites’ sessions prior to conducting their own testing.

Conclusions
Snowball group usability testing provides an efficient method of obtaining multisite feedback on newly developed tools and systems, while addressing barriers typically associated with traditional usability testing methods. This method can be applied to test a wide variety of tools, including CDS tools, prior to launch so that they can be efficiently optimized.
Mobile Phone App to Promote Lifestyle Change in People at Risk of Type 2 Diabetes: Feasibility 3-Arm Randomized Controlled Trial
Authors: Skoglund Gyri , Hilde Gunvor , Lunde Pernille , Cruz Naceno Venessa Vera , Fromholt Olsen Cecilie , Blakstad Nilsson Birgitta 
Published: October 2024

Abstract

Background
The use of mobile health interventions, such as apps, are proposed to meet the challenges faced by preventive health care services due to the increasing prevalence of type 2 diabetes (T2D). Thus, we developed and conducted initial feasibility testing of the Plunde app for promoting and monitoring individual goals related to lifestyle change for people at risk of T2D.

Objective
The primary aim of this study was to assess the feasibility of an app for promoting lifestyle change in people at risk of T2D. The secondary aim was to assess recruitment rate, resource requirements, and change in potential outcomes for a full scale randomized controlled trial (RCT) study .

Methods
A 3-arm feasibility RCT lasting 12 weeks was designed. Participants were recruited from 9 general practitioners in Norway. Eligible participants were randomized to either (1) app follow-up; (2) app follow-up and referral to care as usual in Healthy Life Centers; or (3) referral to care as usual in a Healthy Life Center, only. The primary outcome was feasibility and was measured by app adherence (actual usage of the app), the System Usability Scale, and app motivation score gained from a questionnaire designed for this study. Criteria for success were preset based on these measures. Secondary outcomes included recruitment rate, resource requirements, and potential primary outcomes of a full-scale RCT. This included change in body weight, waist circumference, and self-evaluated functional health status, assessed with the Dartmouth Primary Care Cooperative Research Network/World Organization of Family Doctors (COOP/WONCA) functional health assessment chart.

Results
Within 8 months, 9 general practitioners recruited a total of 54 participants, of which 45 were eligble for participation in the study. Mean age was 61 (SD 13) years and 53% (n=24) were female. App adherence was 86%, the mean System Usability Scale score was 87.3 (SD 11.9), and the mean app motivation score was 74.8 (SD 30.3). Throughout the intervention period, health care professionals spent on average 3.0 (SD 1.0) minutes per participant per week providing follow-up. Statistically significant reduction in body weight and waist circumference was shown in group 1 and 3.

Conclusions
Based on the preset criteria for success, the Plunde app is feasible in providing support for lifestyle change. The Plunde app had excellent user satisfaction. The amount of time spent on monitoring and promoting lifestyle change through the app was low; however, the recruitment was slow. Results from this study will guide the development of further research within this field.
Investigation on the Current Situation and Ethical Reflection of ChatGPT Application in Education under the Background of Digitalization
Authors: Sun Guohang , Li Bingqi , Sun Guohang 
Published: October 2024

With the rapid advancement of the digital globalization process, the iterative renewal of educational technology has promoted the reform of teaching tools and the renewal of educational concepts in the field of education. Advanced AI technologies such as ChatGPT play a key role in this process, bringing unprecedented innovation and opportunities to educational practice with their powerful natural language processing capabilities and deep learning techniques.At the same time, the wide application of these technologies has also triggered a series of deep ethical considerations.Therefore, it is particularly important and urgent to conduct an in-depth investigation into the application status of advanced technologies such as ChatGPT in the field of education and to think calmly about the ethical issues involved.