bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative Genomics and Association Analysis Identifies Virulence Genes of Cercospora sojina on Soybean
Authors: Gu Xin , Ding Junjie , Liu Wei , Yang Xiaohe , Yao Liang liang , Gao Xuedong , Zhang Maoming , Yang Shuai , Wen Jingzhi 
Published: August 2019

Abstract
Recently, a new strain of Cercospora sojina (Race 15) has been identified, which has caused the breakdown of resistance in most soybean cultivars in China. Despite this serious yield reduction, little is known about why this strain is more virulent than others. Therefore, we sequenced the Race 15 genome and compared it to the Race 1 genome sequence, as its virulence is significantly lower. We then re-sequenced 30 isolates of C. sojina from different regions to identifying differential virulence genes using genome-wide association analysis (GWAS). The 40.12-Mb Race 15 genome encodes 12,607 predicated genes and contains large numbers of gene clusters that have annotations in 11 different common databases. Comparative genomics revealed that although these two genomes had a large number of homologous genes, their genome structures have evolved to introduce 245 specific genes. The most important 5 candidate virulence genes were located on Contig 3 and Contig 1 and were mainly related to the regulation of metabolic mechanisms and the biosynthesis of bioactive metabolites, thereby putatively affecting fungi self-toxicity and reducing host resistance. Our study provides insight into the genomic basis of C. sojina pathogenicity and its infection mechanism, enabling future studies of this disease.
1α,25-Dihydroxyvitamin D3 targeting of NF-κB suppresses TNF-α induced adhesion molecules expression in human endothelial cells
Authors: Zhang Qingyan , Feng Yuan , Zhou Yishan , Sun Cheng , Zhu Wei , Cao Dongwei , Liu Jing , Jin Bo , Shao Qiuyuan , Tang Tianfeng , Zhang Miao , Jiang Chunming 
Published: August 2019

Abstract
Background Vitamin D and its analogues have been documented to be associated with endothelial dysfunction in various diseases. However, the underlying mechanism remains unknown. Here, we conducted an in vitro study to evaluate the effect of 1α,25-dihydroxyvitamin D3, the active form of vitamin D, on adhesion molecules expression in human endothelial cells. The possible mechanism involved in this process was also explored.Methods Human umbilical vein cells (HUVECs) were cultured and treated according to the experiment requirement. Western Blot and RT-PCR were used to evaluate the expression of vascular cell adhesion molecule-1 (VCAM-1) and E-selectin. ChIP assay, immunofluorescence, Western Blot and co-immunoprecipitation were used to assess the effect of 1α,25-dihydroxyvitamin D3 on NF-κB signaling.Results 1α,25-dihydroxyvitamin D3 inhibited VCAM-1 and E-selectin mRNA and protein expression after TNF-α stimulation. ChIP assay showed that TNF-α increased the p65 binding to the promoter of VCAM-1 and E-selectin, which was suppressed by 1α,25-dihydroxyvitamin D3. 1α,25-dihydroxyvitamin D3 affected TNF-α induced IκBα phosphorylation and p65 NF-κB activation, leading to an inhibition of p65 nuclear translocation. These effects were reversed by a specific vitamin D receptor siRNA (VDR-siRNA). Co-immunoprecipitation revealed that 1α,25-dihydroxyvitamin D3 induced an increased binding of VDR to p65, which inhibited the ability of p65 binding to target gene promoters.Conclusions 1,25-dihydroxyvitamin D3 suppresses TNF-α induced adhesion molecules expression in human endothelial cells by blocking the NF-κB pathway, and this was VDR dependent.
A concept analysis of children with complex health conditions: implications for research and practice
Authors: Azar Rima , Doucet Shelley , Horsman Amanda Rose , Charlton Patricia , Luke Alison , Nagel Daniel A , Hyndman Nicky , Montelpare William J. 
Published: August 2019

Abstract
BACKGROUND: This concept analysis aimed to clarify the meaning of “children with complex health conditions” and endorse a definition to inform future research, policy, and practice. METHODS: Using Walker and Avant’s (2011)’s approach, we refined the search strategy with input from our team, including family representatives. We reviewed the published and grey literature. We also interviewed 84 health, social, and educational stakeholders involved in the care of children with complex health conditions about their use/understanding of the concept. RESULTS: We provided model, borderline, related, and contrary cases for clarification purposes. We identified defining attributes that nuance the concept: (1) conditions and needs’ breadth; (2) uniqueness of each child/condition; (3) varying extent of severity over time; 4) developmental age; and (5) uniqueness of each family/context. Antecedents were chronic physical, mental, developmental, and/or behavioural condition(s). There were individual, family, and system consequences, including fragmented services. CONCLUSIONS: Building on previous definitions, we proposed an iteration that acknowledges the conditions’ changing trajectories as involving one or more chronic condition(s), regardless of type(s), whose trajectories can change over time, requiring services across sectors/settings, oftentimes resulting in a lower quality of life. A strength of this paper is the integration of the stakeholders’/family’s voices into the development of the definition.
Notification and Communication Technology: An Observational Study of Call Light Systems in Nursing Homes
Authors: Ali Haneen , Li Huiyang 
Published: August 2019

Abstract
Background and Objectives: The call light system is one of the major communication technologies that links the nursing home staff to the needs of the residents. By providing residents with the ability to request assistance, the system becomes an indispensable resource for patient-focused healthcare. However, there is little known about how the call light systems are being used in nursing homes and how the system contributes to the safety and the quality of care for seniors. Therefore, the aims of this study are to understand the nursing home staff experience while using the call light systems and to uncover the usability issues associated with the implemented systems.
Method: A mix of 150 hours of hypothetic-deductive (unstructured) and 90 hours of standard-procedure (structured) observational study were conducted in four different nursing homes. The data collected includes insights into the nursing homes work system and the process of locating and responding to call lights.
Results: The data shows that the highest alarm rate is before and after meal times. The nursing staff exceeded the allotted time set by administration 50% of the time. Additionally, the staff canceled 10% of the call lights and did not immediately assist residents due to high workloads. Further, the staff forgot to come back to assist residents over 3% of the time. Usability issues such as broken parts, lack of feedback, lack of prioritization, and low/no discriminability are contributing to the long response time. More than 8% of the time, residents notified the staff about call lights after they waited for a long time, as these residents were left unattended.
Conclusion: Nursing homes that are still using old call light systems risk the continuation of usability issues that can affect performance of the staff and contribute to a decline in staff and resident outcomes. While the healthcare industry has been at the forefront of technological advancements and implementation, it is important to recognize the influence of technology in the quality of service delivery for the elderly population in nursing homes and to the nursing homes staff working conditions.
Identification of circular RNAs hsa-circ-000696 as a novel biomarker for breast cancer
Authors: WANG Libin , LI Xiaohan , WANG Danni , FENG Huimin , MA Fang , CAO Jia , TIAN Jinhai , YU Jingjing , HUANG Qi , MA Rong , WANG Jia , Zhang Xu 
Published: August 2019

Abstract
Background : To investigate the characteristic expression of circular RNAs between breast cancer and para-carcinoma tissue, identified hsa-circ-000696 as a novel biomarker for breast cancer.
Methods: 136 patients were recruited in General Hospital of NingXia Medical Universit. Of which 3 patients tissue for microarray analysis, 20 and 113 patients for circRNA expression in tissue and peripheral blood, respectively. The candidate circRNAs was verified by qPCR in BC patients’ tissue and peripheral blood. GeneSpring 13.0(Agilent) software were used for analysis the circRNA array data. CircRNA structure were performed by circPrimer1.2 software. MiRanda v3.3,RNAhybrid 2.1 and Cytoscape 3.6.0 were utilized as tools to predict the circRNA-miRNA networks. T-test, Curve regression analysis and ROC analysis were performed to determine the diagnostic values of candidate circRNAs. Statistical analysis was performed using SPSS23.0 software.
Results: 2021 differentially expressed circRNAs were identified, of which 546 were upregulated and 1475 were downregulated. Four circRNAs were selected with filter criteria for further verify in BC tissues. Hsa-circ-0006969 was significantly downregulated both in breast cancer tissues and peripheral blood. Hsa-circ-0006969 proved a highest diagnostic value in breast cancer tissues(AUC=0.889), peripheral blood(AUC=0.823) for Grade 1(AUC=0.672), Grade 3(AUC=0.675), ER positive(AUC=0.648), TNM Ⅰ(AUC=0.687) and TNM Ⅱ(AUC=0.769), TNM Ⅲ(AUC=0.757), TNM early stage(Ⅰ, Ⅱ) (AUC=0.751). Hsa-circ-0006969 show more effective diagnostic values of for tumor metastasis (AUC=0.742) compared with CA153 (AUC=0.712).
Conclusion: Has-circ-0006969 could be a novel predictive biomarker for diagnostic and treatment of breast cancer.
BCR/ABL preleukemic fusion gene in subpopulations of hematopoietic stem and progenitor cells from human UCB
Authors: Jakl Lukas , Škorvaga Milan , Durdík Matúš , Košík Pavol , Bérešová Katarína , Jakubíková Jana , Kubeš Miroslav , Holop Marek , Marková Eva , Belyaev Igor 
Published: August 2019

Abstract
Background BCR/ABL preleukemic fusion gene (PFG) is one of the most frequent fusion genes in acute lymphoblastic leukemia (ALL) and was also detected in hematopoietic cells from umbilical cord blood (UCB) of healthy newborns. Since hematopoietic stem/progenitor cells (HSPC) are considered to be a critical cellular target for origination of leukemia, we have studied the presence of BCR/ABL PFG in expanded subpopulations of HSPC and differentiated cells from UCB of those healthy newborns, who have previously been tested positive for BCR/ABL by screening of their UCB mononuclear cells using RT-qPCR and FISH methods. Methods: We isolated cells from human UCB samples positive for BCR/ABL and negative controls. The isolated cells were sorted into 5 hematopoietic and progenitor cell subpopulations. Sorted cells were expanded and analyzed for the presence of BCR/ABL chromosomal translocation and fusion transcripts by FISH and RT-qPCR, respectively. We analyzed BCR/ABL in sorted and expanded subpopulations of UCB using FISH and RT-qPCR Results: We found that the number of BCR/ABL positive cells was similar in each studied subpopulation and the same as in differentiated lymphocytes. Conclusions: Our data showed that there is no specific subpopulation of hematopoietic and progenitor stem cells with an increased leukemogenic potential due to the presence of higher copies of BCR/ABL.
Caveolin-1 promotes tumor cell proliferation and vasculogenic mimicry formation in human glioma
Authors: Chen Wenli , Cheng Xing , Wang Xiaobo , Hu Wenjie , Wang Jinshan , Liao Chuangxin 
Published: August 2019

Abstract
Background: Vasculogenic mimicry (VM) plays an important role in human glioma progression and resistance to antiangiogenic therapy as a compensatory neovascularization mechanism in malignant tumors. Caveolin-1 (Cav-1) has been found to contribute to VM formation. However, it remains largely unknown whether Cav-1 expression correlates with VM in glioma.
Methods: In this study, by performing immnunohistochemical staining of Cav-1 and CD31/Periodic acid–Schiff co-staining, we found that Cav-1 expression and VM formation were positively correlated with each other and both are closely associated with glioma development and progression as evidenced by the presence of cystic tumor, the shortened survival time and advanced-stage glioma in glioma patients with Cav-1 overexpression/increased VM formation, suggesting Cav-1 as a prognostic indicator in glioma.

Results: Gain- and loss-of-function analyses showed that Cav-1 promoted U251 glioma cell proliferation and VM formation in a Matrigel-based 3D culture model. Mechanistically, overexpression of Cav-1 upregulated while knockdown of Cav-1 downregulated the mRNA/protein expression of VM-associated genes including Akt and hypoxia-inducible factor 1α (HIF-1α), respectively, suggesting that the Akt signaling and HIF-1α may be involved in Cav-1-induced glioma cell proliferation and VM formation.

Conclusion: Collectively, our study identifies Cav-1 as an important regulator of glioma cell proliferation and VM formation, contributing to glioma development and progression.
Risk of multi-drug resistant Campylobacter spp. and residual antimicrobials at poultry farms and live bird markets in Bangladesh
Authors: Neogi Sucharit Basu , Islam Md. Mehedul , Islam SK Shaheenur , Akhter A.H.M. Taslima , Sikder Md. Mahmudul Hasan , Yamasaki Shinji , Kabir S. M. Lutful 
Published: August 2019

Abstract
[EXSCINDED]
Abstract

Background
Understanding potential risks of multi-drug resistant (MDR) pathogens from the booming poultry sector is a crucial public health concern, particularly for developing countries. Campylobacter spp. are among the most important zoonotic pathogens associated with MDR infections in poultry and human. This study systematically examined potential risks and associated socio-environmental factors of MDR Campylobacter spp. in poultry farms and live bird markets (LBMs) of Bangladesh.Methods

Microbial culture and PCR-based methods were applied to examine the occurrence and MDR patterns of Campylobacter spp. at hatcheries, broiler farms and LBMs. Antimicrobial residues in broiler meat and liver samples were detected by advanced chromatogrhaphic techniques. A questionnaire based cross-sectional survey was conducted on socio-environmental factors.Results

In poultry farms, Campylobacter spp. was primarily found in broiler cloacal swabs (21/49, 43%), followed by water (8/24, 33%) and broiler meat (8/28, 29%). Remarkably, in live bird markets, Campylobacter spp. was detected in higher prevalence in broiler meat (14/26, 54%), which could have an association with bacterial contamination in water sources (11/21, 52%) and floor (9/21, 43%). Majority isolates of the predominant species, i.e., Campylobacter jejuni (33/47, 70%) and Campylobacter coli (14/24, 58%), were observed to be MDR, showing resistance to amoxycilin, tetracycline and erythromycin, and additionally ciprofloxacin, norfloxacin, streptomycin, and azithromycin. Residual antimicrobials, including oxytetracycline, ciprofloxacin and enrofloxacin, were detected in majority of broiler liver (79%) and meat (62%) samples; and alarmingly, 33% and 19%, respectively, with concentration above acceptable limit. Inadequate personal and environmental hygiene, unscrupulously use of antimicrobials, improper waste disposal, and lack of health surveillance and quarantine facilities of diseased birds were distinguishable anthropogenic risk factors, with local diversity and compound influences on MDR pathogens.Conclusion

The observed large-scale occurrence of MDR C. jejuni and C. coli and residual antimicrobials in poultry value chain reflects an alarming situation for public health in Bangladesh. Potential contamination sources of MDR Campylobacter and the combined influences of diverse socio-environmental risk factors, noted in this study, would aid in developing interventions to minimize the increasing risks of poultry-associated MDR pathogens under ‘One Health’ banner that includes poultry, human and environment perspectives.
Barriers of Antenatal Care Service Utilization in Somali Regional State Using Socio Ecological Model Framework, Eastern Ethiopia, Ethiopia: a Qualitative Study
Authors: Mohammed Abdurehman , Teklu Alula , Beyene Senait , Hashi Abdiwahab , Abebe Zerihun , Gezahegn Wondimagegn , Mulugeta Mengestie , Metaferia Gesit , Shekabdulahi Muktar , Tolera Moti , Gebru Teklemichael 
Published: August 2019

Abstract
Background

A good Antenatal Care during pregnancy is important to ensure the health of the mother and the healthy development of the fetus. In 2016, at Ethiopian Somali region (43.6%) of pregnant mothers had an Antenatal Care (ANC) visit at least once during their last pregnancy and only (11.8%) had a history of Four or more visits for Antenatal Care, which are the lowest rates among all regions in Ethiopia.Objective

The objective of the study was to explore the barriers and determine enabling factors affecting Antenatal Care service utilization in Somali Regional state.Methods

A qualitative exploration was conducted from February 18 to March 11, 2017. A pre-tested semi-structured interview guides, and facility abstractions with checklists were used to collect the data. A total of 31 individual interviews, 12 focus group discussions, and 21 facility abstractions were collected. The population of the region were stratified into three areas based on their settlement characteristics as agro-pastoralist, pastoralist, and urban. Two districts were selected from each category based on their performance. The data was entered, coded, categorized, and analyzed by utilizing Nvivo version 11software. A thematic analysis was conducted using themes that were developed based on the constructs of the socio-ecological model.Results

According to the study, economic constraints, place of residence, mothers’ being lack of awareness, preference of female health care provider, husband’s disapproval, lack of acceptance by the community, absence of full ANC services packages related with shortage of manpower, medical supply in majority of the health facilities and lack of perceived benefits from ANC service were the main barriers of ANC service utilization.Conclusion

Individual, interpersonal, and organizational level factors were the most dominant barriers for ANC service utilization. Therefore, FMOH, regional, zonal and district level health administrators and other stakeholders should design effective IEC, community mobilization and work on supply-related problems to .increase ANC utilization in the region.
Pregnancy outcomes among 31 patients with tetralogy of Fallot
Authors: Wang Kana , Xin Junguo , Wang Xiaodong , Yu Haiyan , Liu Xinghui 
Published: August 2019

Abstract
Background Ttralogy of Fallot (TOF) is a severe type of congenital heart disease (CHD) and confers substantial risk to mother and fetus. However, the outcomes of pregnant women with TOF have not been well studied.Methods Women with TOF who has been seen and/or delivered at our tertiary-care hospital between April 2008 and January 2018 were retrospective reviewed.Results A total of 31 TOF patients with pregnancies were identified in ten-year period. Among these patients, cardiac defects were surgically repaired in 19 cases and remained uncorrected in 12 women. The frequency of miscarriages and preterm birth was greater in the uncorrected group (16.67% vs one, and 50% vs 5.26%, respectively). The percentage of babies who were small for gestational age (SGA) was 41.67% and 10.53% in two groups. The neonatal mortality and fetal mortality were observed in women without correction for TOF, which were 3.23% (1/31) and 6.45(2/31), respectively. Maternal and neonatal risk appeared to be associated with heart functional classifications, pulmonary hypertension and histories of cardiac events such as serious cardiac arrhythmias.Conclusions Obstetric and cardiac complications are more frequently present in the pregnant women with uncorrected TOF. Surgical correction is associated with improved maternal and perinatal outcome.
Digital Postpartum Hemorrhage Management Device (DPHMD)
Authors: Dereje Derartu , Getachew Abeba , Meskelu Birhan , Adamu Christian , Dejene Kidst , Daba Hundessa , Simegn Gizeaddis Lamesgin 
Published: August 2019

Abstract
Baclground

Postpartum hemorrhage (PPH) is an obstetric emergency caused by excessive blood loss that occurs most commonly after the placenta is delivered. PPH can lead to volume depletion, hypovolemic shock, anemia, which is the leading cause of maternal mortality worldwide. Maternal mortality rate in Ethiopia is among the highest in the world with 470 deaths per 100,000 live births. 94% of births in Ethiopia are estimated to occur at home and ten percent of maternal deaths are attributed to PPH. Now a days, physicians use visual estimation to calculate blood loss and provide fluid during delivery. This traditional method is subjective and generally inaccurate.Method

In this project, after delivery blood loss measurement system integrated with fluid delivery and vital sign monitoring method is proposed. The collection and measurement system collects blood loss after delivery and measure the amount of blood loss. The management system continuously monitors the mother’s heart rate, respiratory rate and blood pressure, and integrates the measured blood loss to make decision for providing appropriate fluid and medication necessary for the mothers. The fluid provided for mothers is also controlled automatically.Results

The prototype was built and undergone through different tests and iterations. The proposed device was tested for accuracy, cost effectiveness and ease to use. 91.28% accuracy has been achieved and the prototype was built only with less than 210 USD.Conclusion

The proposed design allows physicinas, especially those in low resource setting, to estimate blood loss and delver fluid accurately. This helps to reduce maternal mortality rate that may occur due to postpartum hemorrhage.
Evolution of comorbidities in people living with HIV between 2004 and 2014: Cross-sectional analyses from ANRS CO3 Aquitaine Cohort
Authors: Bonnet Fabrice , Marec Fabien Le , Leleux Olivier , Gerard Yann , Neau Didier , Lazaro Estibaliz , Duffau Pierre , Caubet Olivier , Vandenhende Marrie-Anne , Mercie Patrick , Cazanave Charles , Dabis Francois 
Published: August 2019

Abstract
Objective The objective of the present study was to describe the evolution of chronic non-AIDS related diseases and their risk factors, in patients living with HIV (PLHIV) included in the French ANRS CO3 Aquitaine prospective cohort, observed both in 2004 and in 2014 in order to improve long-term healthcare management. Patients and Methods The ANRS CO3 Aquitaine cohort prospectively collects epidemiological, clinical, biological and therapeutic data on PLHIV in the French Aquitaine region. Two cross sectional analyses were performed in 2004 and 2014, regarding patient characteristics, HIVRNA and CD4 counts and prevalence of some of the common comorbidities and treatment. Results 2,138 PLHIV (71% male, median age 52.2 years in 2014), who had at least one visit registered in the cohort both in 2004 and 2014 were included in the study. We have observed a significant increase in the prevalence of diagnosed chronic kidney disease (CKD), bone fractures, cardiovascular events (CVE), hypertension, diabetes and dyslipidaemia as well as an increase in treatment or prevention for these conditions (statins, clopidogrel, aspirin). This is also reflected in an increase in the proportion of patients in the high or very high risk groups in the different disease risk scores for CKD, CVE and bone fracture score. Conclusions Between 2004 and 2014, the population aged and experienced an overall higher prevalence of non-HIV related comorbidities, including CKD and CVD. Careful HIV management according to current recommendations, and optimal selection of antiretrovirals, in addition to a good control of risk factors for these comorbidities, through lifestyle improvements and preventative measures, could lead to an early management of these comorbidities and to a continuous improvement of PLHIV health status.