bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dose-dependent decrease in anti-oxidant capacity of whole blood after irradiation: A novel potential marker for biodosimetry
Authors: Sun Lue , Inaba Yohei , Sato Keizo , Hirayama Aki , Tsuboi Koji , Okazaki Ryuji , Chida Koichi , Moritake Takashi 
Published: May 2018

AbstractMany reports have demonstrated that radiation stimulates reactive oxygen species (ROS) production by mitochondria for a few hours to a few days after irradiation. However, these studies were performed using cell lines, and there is a lack of information about redox homeostasis in irradiated animals and humans. Blood redox homeostasis reflects the body condition well and can be used as a diagnostic marker. However, most redox homeostasis studies have focused on plasma or serum, and the anti-oxidant capacity of whole blood has scarcely been investigated. Here, we report changes in the anti-oxidant capacity of whole blood after X-ray irradiation using C57BL/6 J mice. Whole-blood anti-oxidant capacity was measured by electron spin resonance (ESR) spin trapping using a novel spin-trapping agent, 2-diphenylphosphinoyl-2-methyl-3,4-dihydro-2H-pyrrole N-oxide (DPhPMPO). We found that whole-blood anti-oxidant capacity decreased in a dose-dependent manner (correlation factor, r > 0.9; P < 0.05) from 2 to 24 days after irradiation with 0.5–3 Gy. We further found that the red blood cell (RBC) glutathione level decreased and lipid peroxidation level increased in a dose-dependent manner from 2 to 6 days after irradiation. These findings suggest that blood redox state may be a useful biomarker for estimating exposure doses during nuclear and/or radiation accidents.
Combination of anti-vascular agent - DMXAA and HIF-1α inhibitor - digoxin inhibits the growth of melanoma tumors
Authors: Smolarczyk Ryszard , Cichoń Tomasz , Pilny Ewelina , Jarosz-Biej Magdalena , Poczkaj Aleksandra , Kułach Natalia , Szala Stanisław 
Published: May 2018

AbstractVascular disrupting agents as DMXAA inhibit tumor growth only for a short period of time followed by rapid tumor regrowth. Among others, hypoxia and presence of transcription factor HIF-1α are responsible for tumors regrowth. The aim of our study was to investigate the inhibition of murine melanoma growth by combining two agents: anti-vascular - DMXAA and the HIF-1α inhibitor - digoxin and explaining the mechanism of action of this combination. After DMXAA treatment tumor size was reduced only for a limited time. After 7 days regrowth of tumors was observed and number of vessels was increased especially in tumor’s peripheral areas. DMXAA also induced an influx of immune cells: macrophages, CD8+ cytotoxic lymphocytes, NK cells, CD4+ lymphocytes. Administration of digoxin alone inhibited the growth of tumors. Administration of both agents in the proper sequence significantly inhibited the regrowth of tumors better than either agents alone. Combination therapy reduced number of newly formed vessels. In tumors of mice treated with combination therapy, the number of macrophages M1, CD8+ cytotoxic lymphocytes, NK cells and to a lesser extent CD4+ cells was increased. The combination of anti-vascular agents with HIF-1α inhibitors appears to be an effective therapeutic option.
Radiative forcing by light-absorbing aerosols of pyrogenetic iron oxides
Authors: Ito Akinori , Lin Guangxing , Penner Joyce E. 
Published: May 2018

AbstractIron (Fe) oxides in aerosols are known to absorb sun light and heat the atmosphere. However, the radiative forcing (RF) of light-absorbing aerosols of pyrogenetic Fe oxides is ignored in climate models. For the first time, we use a global chemical transport model and a radiative transfer model to estimate the RF by light-absorbing aerosols of pyrogenetic Fe oxides. The model results suggest that strongly absorbing Fe oxides (magnetite) contribute a RF that is about 10% of the RF due to black carbon (BC) over East Asia. The seasonal average of the RF due to dark Fe-rich mineral particles over East Asia (0.4–1.0 W m−2) is comparable to that over major biomass burning regions. This additional warming effect is amplified over polluted regions where the iron and steel industries have been recently developed. These findings may have important implications for the projection of the climate change, due to the rapid growth in energy consumption of the heavy industry in newly developing countries.
MiR-23a transcriptional activated by Runx2 increases metastatic potential of mouse hepatoma cell via directly targeting Mgat3
Authors: Huang Huang , Liu Yubo , Yu Peishan , Qu Jianhua , Guo Yanjie , Li Wenli , Wang Shujing , Zhang Jianing 
Published: May 2018

AbstractMicroRNAs (miRNAs) and aberrant glycosylation both play important roles in tumor metastasis. In this study, the role of miR-23a in N-glycosylation and the metastasis of mouse hepatocellular carcinoma (HCC) cells was investigated. The miRNA expression array profiles that were confirmed by qPCR and Western blot analyses revealed higher miR-23a expression levels in Hca-P cells (with lymphatic metastasis potential) than in Hepa1–6 cells (with no lymphatic metastasis potential), while the expression of mannoside acetylglucosaminyltransferase 3 (Mgat3) was negatively associated with metastasis potential. Mgat3 is a key glycosyltransferase in the synthesis of the bisecting (β1,4GlcNAc branching) N-glycan structure. Bioinformatics analysis indicated that Mgat3 may be a target of miR-23a, and this hypothesis was verified by dual-luciferase reporter gene assays. Furthermore, we found that the transcription factor Runx2 can directly bind to the miR-23a gene promoter and promote its expression, as shown in dual-luciferase reporter gene assays and ChIP assays. Collectively, these results indicate that miR-23a might increase the metastatic potential of mouse HCC by affecting the branch formation of N-glycan chains presented on the cell surface through the targeting of the glycosyltransferase Mgat3. These findings may provide insight into the relationship between abnormal miRNA expression and aberrant glycosylation during tumor lymphatic metastasis.
Her2 Ile655Val polymorphism and its association with breast cancer risk: an updated meta-analysis of case-control studies
Authors: Krishna B. Madhu , Chaudhary Sanjib , Panda Aditya K. , Mishra Dipti Ranjan , Mishra Sandip K. 
Published: May 2018

AbstractBreast cancer (BC) is one of the most common types of cancer in women worldwide. Several factors including genetic and environmental have been linked with susceptibility to development of BC. Her2 is a transmembrane protein with tyrosine kinase activity, overexpressed in several cancers including BC. Various studies in different populations have shown association of Her2 variants with susceptibility to BC, however these results were inconsistent, inconclusive and controversial. To obtain a common conclusive finding, we performed meta-analysis of 35 case-control studies reported earlier including 19, 220 cases and 22, 306 controls. We observed significant association of Her2Ile655Val polymorphism with susceptibility to development of breast cancer (Overall allele Val vs Ile: OR = 1.130, 95% CI = 1.051–1.216, p = 0.001; Ile-Val vs Ile-Ile: OR = 1.100, 95% CI = 1.016–1.192, p = 0.019; Val-Val+Ile-Val vs Ile-Ile: OR = 1.127, 95% CI = 1.038–1.223, p = 0.004). Subgroup analysis indicated a significant association with susceptibility to breast cancer in African and Asian populations. However, such association was not observed in other ethnic groups. Our findings suggested that Her2Ile655Val polymorphism is associated with breast cancer risk in overall, Asian and African populations, and can be used as diagnostic marker for BC.
Sporulation-specific cell division defects in ylmE mutants of Streptomyces coelicolor are rescued by additional deletion of ylmD
Authors: Zhang Le , Willemse Joost , Hoskisson Paul A. , van Wezel Gilles P. 
Published: May 2018

AbstractCell division during the reproductive phase of theStreptomyceslife-cycle requires tight coordination between synchronous formation of multiple septa and DNA segregation. One remarkable difference with most other bacterial systems is that cell division inStreptomycesis positively controlled by the recruitment of FtsZ by SsgB. Here we show that deletion ofylmD(SCO2081) orylmE(SCO2080), which lie in operon withftsZin thedcwcluster of actinomycetes, has major consequences for sporulation-specific cell division inStreptomyces coelicolor. Electron and fluorescence microscopy demonstrated thatylmEmutants have a highly aberrant phenotype with defective septum synthesis, and produce very few spores with low viability and high heat sensitivity. FtsZ-ring formation was also highly disturbed inylmEmutants. Deletion ofylmDhad a far less severe effect on sporulation. Interestingly, the additional deletion ofylmDrestored sporulation to theylmEnull mutant. YlmD and YlmE are not part of the divisome, but instead localize diffusely in aerial hyphae, with differential intensity throughout the sporogenic part of the hyphae. Taken together, our work reveals a function for YlmD and YlmE in the control of sporulation-specific cell division inS. coelicolor, whereby the presence of YlmD alone results in major developmental defects.
Transdermal water-in-oil nanocarriers of nitric oxide for triggering penile erection
Authors: Nam Eunryel , Yoo Saejong , Kim Hwi-Yool , Kim Young-Rok , Heo Yun Jung 
Published: May 2018

AbstractMen’s sexual health can have significant effects on a man’s self-esteem, sexual relationship and male reproductive functions. Although commercially available drugs (e.g., VIAGRA and CIALIS) show effective treatment of erectile dysfunction (ED), patients with severe ED fail to respond to these medicines. Topical nitric-oxide (NO) delivery to penis can be a painless, alternative solution with severe ED because NO triggers erection and diffuses to the trabecular arteries and smooth muscles in the penis. We here develop water-in-oil (W/O) nanoemulsions (NEs) that contain NO and can directly spread on the penis. We optimize NE formation conditions including hydrophilic-lipophilic balance (HLB) and ratio of oil, water and surfactants. Then, by spreading NEs on penis skin of intact middle aged dogs, we verify medication effects and safety of the NEs in vivo. The water-in-oil NEs can be a promising non-invasive medication for ED patients with low response to a phosphodiesterase type 5 (PDE5) inhibitor, thus increasing quality of life in the aging society.
A novel method for expansion and differentiation of mouse tracheal epithelial cells in culture
Authors: Eenjes Evelien , Mertens Tinne C. J. , Buscop-van Kempen Marjon J. , van Wijck Yolanda , Taube Christian , Rottier Robbert J. , Hiemstra Pieter S. 
Published: May 2018

AbstractAir-liquid interface (ALI) cultures of mouse tracheal epithelial cells (MTEC) are a well-established model to study airway epithelial cells, but current methods require large numbers of animals which is unwanted in view of the 3R principle and introduces variation. Moreover, stringent breeding schemes are frequently needed to generate sufficient numbers of genetically modified animals. Current protocols do not incorporate expansion of MTEC, and therefore we developed a protocol to expand MTEC while maintaining their differentiation capacity. MTEC were isolated and expanded using the ROCK inhibitor Y-27632 in presence or absence of the γ-secretase inhibitor DAPT, a Notch pathway inhibitor. Whereas MTEC proliferated without DAPT, growth rate and cell morphology improved in presence of DAPT. ALI-induced differentiation of expanded MTEC resulted in an altered capacity of basal cells to differentiate into ciliated cells, whereas IL-13-induced goblet cell differentiation remained unaffected. Ciliated cell differentiation improved by prolonging the ALI differentiation or by adding DAPT, suggesting that basal cells retain their ability to differentiate. This technique using expansion of MTEC and subsequent ALI differentiation drastically reduces animal numbers and costs for in vitro experiments, and will reduce biological variation. Additionally, we provide novel insights in the dynamics of basal cell populations in vitro.
In vivo Molecular Imaging of Glutamate Carboxypeptidase II Expression in Re-endothelialisation after Percutaneous Balloon Denudation in a Rat Model
Authors: Endepols Heike , Mottaghy Felix M. , Simsekyilmaz Sakine , Bucerius Jan , Vogt Felix , Winz Oliver , Richarz Raphael , Krapf Philipp , Neumaier Bernd , Zlatopolskiy Boris D. , Morgenroth Agnieszka 
Published: May 2018

AbstractThe short- and long-term success of intravascular stents depends on a proper re-endothelialisation after the intervention-induced endothelial denudation. The aim of this study was to evaluate the potential of in vivo molecular imaging of glutamate carboxypeptidase II (GCPII; identical with prostate-specific membrane antigen PSMA) expression as a marker of re-endothelialisation. Fifteen Sprague Dawley rats underwent unilateral balloon angioplasty of the common carotid artery (CCA). Positron emission tomography (PET) using the GCPII-targeting tracer [18F]DCFPyL was performed after 5–21 days (scan 60–120 min post injection). In two animals, the GCPII inhibitor PMPA (23 mg/kg BW) was added to the tracer solution. After PET, both CCAs were removed, dissected, and immunostained with the GCPII specific antibody YPSMA-1. Difference of GCPII expression between both CCAs was established by PCR analysis. [18F]DCFPyL uptake was significantly higher in the ipsilateral compared to the contralateral CCA with an ipsi-/contralateral ratio of 1.67 ± 0.39. PMPA blocked tracer binding. The selective expression of GCPII in endothelial cells of the treated CCA was confirmed by immunohistological staining. PCR analysis verified the site-specific GCPII expression. By using a molecular imaging marker of GCPII expression, we provide the first non-invasive in vivo delineation of re-endothelialisation after angioplasty.
10 GHz highly linear up‐conversion mixer in 65 nm CMOS
Authors: Li J. , Gu Q.J. 
Published: May 2018

A highly linear folded‐type up‐conversion mixer working at 10 GHz is presented. The mixer utilises a feedback loop to linearise the gm stage and make the effective gm only determined by a resistor theoretically. Moreover, the effects of local oscillator (LO) power levels on the circuit DC condition and linearity performances are examined from both the theoretical analysis and measurement results. The mixer prototype, fabricated in a 65 nm CMOS technology, consumes 7.7 mW from 1.2 V supply voltage and exhibits 13.4 dBm OIP3 at the LO power of 2 dBm.
40 Gbps 4‐level pulse amplitude modulation closed‐loop decision‐feedback equaliser with high‐speed comparator in 55 nm CMOS technology
Authors: He Ai , Gai Weixin , Tang Liangxiao 
Published: May 2018

A 40 Gbps 4‐tap 4‐level pulse amplitude modulation closed‐loop decision feedback equaliser (DFE) is proposed. The DFE adopts a novel high‐speed comparator to resolve the critical timing constraints of the first tap. The comparator decreases the slicing delay by shortening the gap between initial and target voltages. Compared with the existing closed‐loop DFE designs, the proposed scheme relieves timing constraints without complex clock distribution circuits and extra area. Simulations based on the RF‐MOS model verify that the delay of the comparator is improved by 32.8% and the output swing is increased by more than 2.8 times. The proposed DFE which can compensate −9.5 dB channel loss is designed in 55 nm CMOS technology. The power consumption is 67 mW from a 1.2 V supply and the circuit occupies an active area of 0.021 mm2, achieving 1.68 pJ/bit energy efficiency.
Simple dual‐mode balanced bandpass filter with high selectivity and extended common‐mode noise suppression
Authors: Gu Hui , Ge Lei , Xu Lijie 
Published: May 2018

A new miniaturised dual‐mode balanced bandpass filter (BPF) is presented in this letter. To achieve the required differential‐mode (DM) operation with common‐mode (CM) suppression, an octagonal microstrip dual‐mode loop resonator is employed. By symmetrically locating four high‐low impedance sections inside the free area of the loop resonator, the proposed balanced BPF can realise compact size, wide stopband under DM operation, and extended CM suppression, simultaneously. Furthermore, a pair of coupled lines is introduced to readily achieve two transmission zeroes for realising high selectivity of DM response. For demonstration, a dual‐mode balanced BPF with the passband centred at 2.39 GHz is designed and measured. Both the simulated and measured results are provided with good agreement.
Reanimation unter besonderen Umständen: Kreislaufstillstand bei implantiertem Linksherzassist-Device (LVAD)
Authors: Thiele Jochen , Matusch Dennis , Reifferscheid Florian 
Published: May 2018

ZusammenfassungNach einer körperlichen Auseinandersetzung in einem Bus des ÖPNV bricht eine Person reanimationspflichtig zusammen. Der eintreffenden RTW-Besatzung fällt eine Umhängetasche des Patienten auf, die einen lauten Pfeifton abgibt. Die körperliche Untersuchung und Inspektion der Umhängetasche durch den Notarzt ergibt als Ursache für den Pfeifton eine diskonnektierte Driveline eines Linksherzunterstützungssystems („left ventricular assist device“ = LVAD). Die Driveline wird wieder mit dem Kontroller in der Umhängetasche konnektiert. Die folgende Analyse des Herzrhythmus zeigt keine eindeutige elektrische Herzaktion, ein noninvasiver Blutdruck ist nicht messbar, der Patient bleibt bewusstlos. Das endtidale CO2 lag unter volumenkontrollierter Beatmung bei 35 mmHg. Aufgrund dieses Wertes wird zu diesem Zeitpunkt der ROSC definiert. Nach einer 24-stündigen Kühlung kann der Patient problemlos extubiert werden. Eine kardiochirurgische Intervention ist nicht notwendig. Aufgrund steigender Zahlen implantierter kardialer Assistsysteme ist mit einer Zunahme von Patienten im Rettungsdienst zu rechnen. Neben den Standardmaßnahmen der Reanimation kommt dem Beheben der Ursache des Kreislaufstillstandes höchste Priorität zu. Das Erheben von Vitalparametern ist aufgrund der fehlenden Pulsatilität eingeschränkt bis unmöglich. Die Messung des endtidalen CO2 kann hier zur Beurteilung der Kreislaufsituation genutzt werden.
Implementing, Connecting, and Evaluating a Standard-Based Integrated Operating Room within a German University Hospital
Authors: Dees Raluca , Merzweiler Angela , Schneider Gerd , Kasparick Martin , Mündermann Lars , Ahlbrandt Janko , Wagner Martin , Kenngott Hannes , Müller-Stich Beat , Bergh Björn 
Published: May 2018

Background Digital operating rooms (ORs), when optimally designed and integrated, can reduce the complexity of the surgery suite. However, many integrated ORs are effectively isolated from other IT systems in the hospital because there is little or no connectivity with them. Within the German flagship project OR.NET, concepts and components were developed for a standard-based connection of the OR with hospital IT systems.
Objectives The aim of this work was to implement and evaluate OR.NET concepts and components within the existing IT landscape of a German university hospital. This article describes and evaluates the implemented architecture and processes for connecting a demo OR to existing hospital IT systems at Heidelberg University Hospital.
Methods For the design, establishment, and evaluation of standard-based connections of the demo OR with hospital IT systems, the iterative method “Design and Creation” with four iterations was applied.
Results A generic and a concrete architecture for several standard-based connection concepts of the demo OR were developed. Furthermore, the concrete architecture was implemented and evaluated for its technical and clinical relevance. The main benefits of the project were the establishment of basic requisites for improving the efficiency within the OR, easier operation of medical devices as a result of harmonized human–machine interfaces, and providing additional data for improving healthcare.
Conclusion OR.NET concepts for a standard-based connection of the OR with hospital IT systems have proven to be promising. They can serve as a reference for further integration scenarios in other hospitals.
Impact of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight for the Identification of Gram-Positive Bloodstream Pathogens without Antimicrobial Stewardship Intervention in Hospitalized Children
Authors: Herrera Lisa , Culbreath Karissa , Dehority Walter 
Published: May 2018

AbstractWe performed a 2-year retrospective cohort study of inpatient children with growth of gram-positive bacteria from blood cultures identified with and without the use of matrix-assisted laser-desorption/ionization time-of-flight (MALDI-TOF) in the absence of an antimicrobial stewardship program. Use of MALDI-TOF reduced the time to organism identification by 15.7 hours (p < 0.0001). However, no differences in length of stay, time to optimization of antimicrobial therapy, or time to discontinuation of antibiotics for growth of contaminants were documented, suggesting that such potential patient-oriented benefits of MALDI-TOF may only be evident in the presence of a concurrent antimicrobial stewardship program.
Overcoming Tortuous Anatomy in Intracranial Intervention
Authors: Cherian Mathew , Mehta Pankaj , Santhosh Poyyamoli , Rahul Kareparambil , Jenny Gandhi , Elango Swamiappan 
Published: May 2018

AbstractIntracranial interventions comprise a set of procedures, which are complex and challenging. Further, they are extremely risky because complications in the brain are usually associated with significant mortality and morbidity. Thus, a clear understanding is necessary to handle tortuous vessels because improper planning can result in a higher rate of complications. The combination of long sheaths, specialty wires, and preshaped catheters can enable us to access some of the most complex vascular anatomy. There are several conditions that can contribute to a difficult access. There are few techniques described by different authors. The authors have listed the techniques found useful based on their experience. These challenges include tortuous iliac arteries, dilated tortuous aortic arch, acute takeoff of the arch vessels, tortuous neck vessels with prominent loops, crossing a large aneurysm, extensive peripheral vascular disease, etc.