bluuebunny/crossref_metadata_2025_split huggingface dataset:
Proyectos de emprendimiento productivo, mecanismo para mejorar la motivación actitudinal en los estudiantes de bachillerato
Authors: Yépez Alvarado Rosa Marisol , Jaya Torres Patricia Isolina , Guzmán Hernández Ramón , Maceo Castillo Luis Manuel 
Published: November 2024

Introducción: la motivación actitudinal es crucial para el desarrollo integral de los estudiantes de bachillerato. Muchos de ellos enfrentan dificultades para aplicar los conocimientos teóricos en la práctica, lo que afecta su interés académico. Este estudio busca mejorar dicha motivación a través de un proyecto de emprendimiento productivo. Objetivo: fomentar el espíritu emprendedor en los estudiantes, brindándoles herramientas que les permitan identificar oportunidades de negocio, desarrollar estrategias y ejecutar ideas emprendedoras. Metodología: se realizó un estudio con un grupo experimental, que participó en talleres prácticos, y un grupo de control que siguió con el currículo tradicional. Se evaluó la motivación y el rendimiento académico mediante una evaluación continua. Resultados: el grupo experimental mostró una mayor motivación e interés en sus estudios, destacando la importancia del aprendizaje práctico. Además, su rendimiento académico y habilidades emprendedoras mejoraron notablemente en comparación con el grupo de control. Conclusión: el proyecto de emprendimiento productivo fue eficaz para mejorar tanto la motivación actitudinal como el rendimiento académico, proporcionando herramientas útiles para la vida personal y profesional de los estudiantes. Área de estudio general: Educación. Área de estudio específica: Emprendimiento educativo. Tipo de estudio: Artículos originales.
Application of percutaneous neurostimulation in treatment complexes for psychosomatic pathology
Authors: Chesnikova E. I. , Bokova I. A. , Konchugova T. V. , Agasarov L. G. 
Published: November 2024

The article is devoted to the combined use of psychological relaxation and transcutaneous electrical stimulation in post-stress disorders in persons of hazardous professions.Materials and methods. There were 90 men with a high level of stress aged 40 to 55 years, whose clinical picture was distinguished by the interweaving of neuropsychiatric and cardiovascular disorders. The assessment of the mental status of patients was supplemented by the results of testing; the reserve capabilities of the body were assessed based on a test of dosed physical activity. Electro- and echocardiography were used to analyze cardiovascular activity. The patients were divided into three treatment groups, in which, in addition to the previously prescribed drug treatment in a third of cases, respiratory relaxation training and percutaneous neurostimulation were used. In the first two groups, these methods were used separately, in the 3rd group – in combination.Research results. Initially, the features of neuropsychic and somatic manifestations in the observed contingent were specified. At the same time, in most of the observations, neurotic manifestations prevailed, combined with somatic disorders, mainly in the form of arterial hypertension and angina pectoris. In the course of assessing the results of treatment, the proposed complex was significantly more effective than the compared groups in terms of improving indicators of mental status and cardiovascular activity. The catamnesis analysis confirmed the stability of the results achieved even in the case of combined exposure.Conclusion. The therapeutic complex of psychorelaxation and hardware equipment provides the effectiveness and therapeutic reliability of the effect due to the addition of several therapeutic mechanisms, and the work itself opens up prospects for optimizing corrective programs for the pathology under study.
A novel, rapid, ultrasensitive diagnosis platform for detecting Candida albicans using restriction endonuclease‐mediated real-time loop-mediated isothermal amplification
Authors: Wang Yizhe , Zhou Yuhong , Lu Jingrun , Yu Honglan , Wang Yu 
Published: November 2024

IntroductionCandida albicans (C. albicans, CA) is an essential invasive fungus in clinical diagnosis. Although several detection methods exist, none meet the need for early diagnosis. A rapid, sensitive, and specific diagnostic tool is crucial for effective prevention and control of C. albicans infections.MethodsThis study aimed to develop a new, rapid, and ultrasensitive diagnostic tool for C. albicans detection based on restriction endonuclease-mediated real-time loop-mediated isothermal amplification (ERT-LAMP-CA). The ERT-LAMP-CA technology combines LAMP amplification, restriction endonuclease cleavage, and real-time fluorescence detection in a single reaction tube, which can complete a diagnosis of C. albicans in a short time (approximately 1 h).ResultsHerein, we developed the primer sequences required for ERT-LAMP-CA based on the ITS2 gene of C. albicans and found that ERT-LAMP-CA limit of detection was approximately 500 ag/μL genomic DNA and can present negative results for non-C. albicans templates. We tested sputum samples from 64 patients with suspected C. albicans infections to validate ERT-LAMP-CA applicability in clinical sample testing and found that ERT-LAMP-CA was consistent with multiplex PCR-capillary electrophoresis.DiscussionIn conclusion, ERT-LAMP-CA is a rapid, accurate, and sensitive assay with excellent potential for clinical and basic laboratory diagnosis and an efficient screening strategy.
Catheter ablation of atrial fibrillation in women with heart failure with preserved ejection fraction
Authors: Zylla Maura M. , Leiner Johannes , Rahm Ann-Kathrin , Hoffmann Tobias , Lugenbiel Patrick , Schweizer Patrick , Mages Christine , Mereles Derliz , Kieser Meinhard , Scholz Eberhard , Katus Hugo A. , Frey Norbert , Thomas Dierk 
Published: November 2024

BackgroundHeart failure with preserved ejection fraction (HFpEF) and atrial fibrillation (AF) often coincide. Female sex is associated with both increased prevalence of HFpEF and reduced therapeutic efficacy of catheter ablation of AF. This sub-analysis of the previously published AFFECT-study evaluates outcome after cryoballoon-ablation in women with and without HFpEF.MethodsOne-hundred-and-two patients (LVEF ≥ 50%) scheduled for cryoballoon-ablation of AF were prospectively enrolled. Forty-two were female. Comprehensive baseline assessment included echocardiography, stress echocardiography, six-minute-walk-test, biomarker- and quality-of-life-assessment (QoL, SF-36), and was repeated at follow-up ≥12 months after AF-ablation. Baseline parameters, procedural characteristics and outcome after AF-ablation were compared between women with and without HFpEF.ResultsWomen with HFpEF (n = 20) were characterized by higher median left atrial volume index (35.8 ml/m2 vs. 25.8 ml/m2, P &lt; 0.001), left ventricular hypertrophy (median left ventricular mass index: 92.0 g/m2 vs. 83.0 g/m2, P = 0.027), reduced distance in the 6-min-walk-test (median: 453 m vs. 527 m, P = 0.008) and higher left atrial pressures (median: 14.0 mmHg vs.9.5 mmHg, P = 0.008) compared to women without HFpEF (n = 21). During follow-up, HFpEF-patients more often experienced AF-related re-hospitalization (36.8% vs. 9.1%, P = 0.039) and numerically higher AF-recurrence-rates (57.9% vs. 31.1%, P = 0.109). There was no significant improvement of heart failure-related symptoms, echocardiographic parameters and cardiac biomarkers levels. QoL showed no significant improvement in both subgroups. Women with HFpEF still exhibited a lower SF-36 Physical Component Summary Score vs. women without HFpEF (median: 41.2 vs. 52.1, P &lt; 0.001).ConclusionWomen with HFpEF constitute a distinct subgroup with high rates of AF-related events after AF-ablation, and persistence of both symptoms and functional hallmarks of HFpEF. Consideration of sex-specific cardiac co-morbidities is crucial for personalization and optimization of AF-therapy.Clinical Trial RegistrationClinicalTrials.gov Identifier NCT05603611.
The serum uric acid/high-density lipoprotein cholesterol ratio: a novel predictor for the presence of abdominal aortic aneurysm
Authors: Li Wei , Luo Songyuan , Lin Wenhui , Hu Xiaolu , Zhou Dan , Xu Wenmin , Zhou Yingling , Luo Jianfang , Feng Yingqing 
Published: November 2024

ObjectiveRobust evidence has demonstrated that inflammation plays an important role in the occurrence and development of abdominal aortic aneurysms (AAA). The serum uric acid (UA)/high-density lipoprotein cholesterol (HDL-C) ratio (UHR) has recently been recognized as a new biomarker for evaluating inflammatory and anti-inflammatory interactions. However, whether UHR is associated with AAA remains unclear. This study aimed to explore the association between UHR and presence of AAA.MethodsWe prospectively performed a hospital-based and community-based AAA screening program using ultrasonography in 9,064 individuals at Guangdong Provincial People’s Hospital and two communities in China. Logistic regression analysis was used to explore the association between UHR and presence of AAA. In addition, the restricted cubic spline (RCS) regression method was used to visually investigate the dose-response relationship between UHR and the presence of AAA. Propensity score matching (PSM) analysis was conducted to adjust for baseline variations and diminish selection bias, and subgroup analysis was performed to investigate the consistency of the conclusions.ResultsThe prevalence of AAA was 2.45% (222/9,064) in the present study. The optimal cut-off value of UHR was 17.0%, which was selected according to the receiver operator characteristic curve. The prevalence of AAA was 3.96% in the high-UHR group (UHR ≥ 17%) and 1.54% in the low-UHR group (UHR &lt; 17%) (P &lt; 0.001). After adjusting for other relevant clinical covariates, UHR was independently associated with the presence of AAA, either as a continuous variable (odds ratio [OR] 1.03, 95% confidence intervals [CI] 1.01–1.05, P &lt; 0.001) or as a categorical variable (OR 1.63, 95% CI 1.18–2.26, P = 0.003). The RCS curve showed a nonlinear dose-response relationship between UHR and the presence of AAA. Moreover, the positive correlation between UHR and the presence of AAA remained significant after PSM and subgroup analyses.ConclusionsUHR was positively associated with the presence of AAA, and there was a non-linear dose-response relationship between them. Thus, UHR may serve as a novel and reliable predictor of AAA.
Combined high-intensity interval and resistance training improves cardiorespiratory fitness more than high-intensity interval training in young women with overweight/obesity: a randomized controlled trial
Authors: Wang Yifei , Yang Xin , Deng Jiamei , Wang Zhenshan , Yang Dongxue , Han Yanbai , Wang Hongli 
Published: November 2024

ObjectiveThis study aimed to compare the effects of high-intensity interval training (HIIT) combined with resistance training (RT) versus HIIT alone on body composition, cardiorespiratory fitness and glycolipid metabolism in young women with overweight/obesity.MethodsThis randomized controlled trial divided 40 subjects into an experimental group (HIIT+RT) and a control group (HIIT). Both groups underwent training three times per week for eight weeks. Body composition, cardiorespiratory fitness and glycolipid levels were assessed before and after the intervention.ResultsThe results revealed that compared to baseline, both the experimental and control groups showed significant improvements in body weight, body mass index (BMI), Body fat, waist circumference (WC), waist-hip ratio (WHR), peak oxygen uptake (VO2peak), vital capacity (VC), oxygen pulse (VO2/HR), minute ventilation, resting heart rate, blood oxygen saturation, blood pressure, fasting blood glucose, triglycerides and high-density lipoprotein cholesterol (p&lt;0.05). Additionally, a significant increase in muscle mass and a significant reduction in 2-hour postprandial glucose were observed in the experimental group (p&lt;0.05). Muscle mass (mean difference: 2.75%), VO2peak (mean difference: 1.61 mL/min/kg), VC (mean difference: 334mL), and VO2/HR (mean difference: 0.51mL/beat) showed greater improvement in the HIIT+RT group compared to the HIIT group (p&lt;0.05).ConclusionIn conclusion, an 8-week regimen of either combined HIIT and RT or HIIT significantly improves body composition, cardiorespiratory fitness and glycolipid metabolism in women with overweight/obesity. However, the combined training appears to offer more benefits than HIIT alone. Further research is needed to evaluate the long-term effects and feasibility of combined training.Trial registrationhttps://www.chictr.org.cn/, identifier ChiCTR2300075961.
Spatial variation in water quality of the Burhi Gandak River: a multi-location assessment
Authors: Priyadarshee Akash , Rahul Atul Kumar , Kumar Vijay , Kumar Ashish , Kumar Niraj 
Published: November 2024

The Burhi Gandak River, a significant tributary of the Ganga River and a vital water source in Bihar, India, is facing critical water quality degradation due to rapid population growth, urbanization, and industrial activities. This study conducts a detailed water quality assessment of the river, focusing on the effects of these anthropogenic factors across different locations, seasons, and industrial zones. Water samples were collected and analyzed for key parameters, including pH, total dissolved solids (TDSs), total hardness (TH), biological oxygen demand (BOD), and dissolved oxygen (DO). The results indicate significant spatial and temporal variations, with water quality deteriorating notably in areas like Samastipur and Khagaria, where industrial and urban activities are more concentrated. For instance, BOD levels increased from 7.5 mg/L to 9.5 mg/l as the river flows through urban Samastipur, signaling a decline in water quality. Additionally, sugar mills located along the river contribute to higher pollution levels during operational months, especially in Lauria and Sugauli. Seasonal analysis shows that the lean season experiences the highest levels of degradation, while monsoon floods increase suspended solids due to sediment inflow. These findings emphasize the urgent need for improved regulation of industrial discharge and urban waste management to protect the water quality of the Burhi Gandak River and the livelihoods dependent on it.
Implementation of a national rapid prenatal exome sequencing service in England: evaluation of service outcomes and factors associated with regional variation
Authors: Ramakrishnan Rema , Mallinson Corinne , Hardy Steven , Broughan Jennifer , Blyth Maisie , Melis Gabriella , Franklin Catherine , Hill Melissa , Mellis Rhiannon , Wu Wing Han , Allen Stephanie , Chitty Lyn S. , Knight Marian ,   EXPRESS Clinical Outcomes Group
Published: November 2024

IntroductionPrenatal exome sequencing (pES) can enhance genetic diagnosis of fetuses with structural anomalies and has recently been introduced as a national service in England. We aimed to examine service outcomes such as diagnostic yield (definite final diagnosis), referral rate, and sources of referral, and explore variation in outcomes of pES by individual or service level factors between 01 October 2021 and 30 June 2022.MethodspES testing results from the National Health Service laboratories performing testing were linked to National Congenital Anomaly and Rare Disease Registration Service data and the Maternity Services Data Set and descriptive statistics computed.ResultsThere were 475,089 women who gave birth in England during the study period. The referral rate for pES was 8.6 (95% CI 7.8, 9.4) per 10,000 maternities. About 59% of those referred proceeded with pES testing and 35% of women who proceeded received a definite final diagnosis with a median turnaround time of 15 days. Of those who had pES testing, 64.6% had a live birth, 25.3% underwent termination of pregnancy (median gestational age at termination: 26 weeks), and 9.3% had a stillbirth. Among the 85 women who had a definite final diagnosis, 40% had a termination of pregnancy, 18% had a stillbirth, and 42% had a live birth. The corresponding figures among women without a definite final diagnosis were 18%, 5%, and 78%, respectively. Among women who had a termination of pregnancy, the median gestational age at final report was 24.9 weeks and 26.2 weeks at termination. Variation observed in some of the characteristics and outcomes between regional services were limited by small sample size.ConclusionThis study showed that of those referred, pES testing provided a diagnosis for one in three pregnancies with a fetal anomaly across England during the study period when other tests had been non-informative. Women who opted for a termination of pregnancy underwent the procedure at relatively late gestations. Earlier referral for pES, streamlining pathways, and faster turnaround times may help results to be available at an earlier gestation to allow families more time to make decisions around continuing or terminating their pregnancy. The variation in service outcomes between regional services needs to be investigated further to understand the reasons for these differences.
CXCR4 antagonism ameliorates leukocyte abnormalities in a preclinical model of WHIM syndrome
Authors: Roland Lilian , Nguyen Chi Huu , Zmajkovicova Katarina , Khamyath Mélanie , Kalogeraki Maria , Schell Bérénice , Gourhand Vanessa , Rondeau Vincent , Abou Nader Zeina , Monticelli Halenya , Maierhofer Barbara , Johnson Robert , Taveras Arthur , Espéli Marion , Balabanian Karl 
Published: November 2024

BackgroundWHIM (Warts, Hypogammaglobulinemia, Infections, and Myelokathexis) syndrome is an ultra-rare, combined primary immunodeficiency and chronic neutropenic disorder characterized by a range of clinical presentations, including peripheral neutropenia, lymphopenia, and recurrent infections. WHIM syndrome is most often caused by gain-of-function mutations in the gene encoding C-X-C chemokine receptor 4 (CXCR4). As such, inhibition of CXCR4 with XOLREMDI® (mavorixafor), an orally bioavailable CXCR4 antagonist, demonstrated clinically meaningful increases in absolute neutrophil and lymphocyte counts and concomitant reduction in infections in patients with WHIM syndrome, resulting in its recent U.S. Food and Drug Administration approval. The impact of CXCR4 antagonism on other aspects of the pathobiology in WHIM syndrome, such as lymphopoiesis and leukocyte trafficking between primary and secondary lymphoid organs, is less understood.MethodsIn the current study, the effects of CXCR4 antagonism on leukocyte trafficking and distribution in primary and secondary lymphoid organs were investigated in a mouse model of WHIM syndrome carrying the heterozygous Cxcr41013 mutation. Cxcr4+/1013 and Cxcr4 wild-type mice received the orally bioavailable CXCR4 antagonist X4-185. Blood, spleen and bone marrow samples were collected for numeration, flow cytometry, and functional studies.ResultsCxcr4+/1013 mice exhibited profound peripheral blood leukopenia as seen in patients with WHIM syndrome. CXCR4 antagonism corrected circulating leukopenia and mobilized functional neutrophils without disrupting granulopoiesis in the bone marrow of Cxcr4+/1013 mice. Furthermore, Cxcr4+/1013 displayed aberrant splenic T and B-cell counts and frequency. Treatment with X4-185 normalized splenic T-cell abnormalities, correcting the reduced CD8+ T-cell numbers, restoring the CD4/CD8 T-cell ratio, and ameliorating peripheral blood T-cell lymphopenia. In addition, CXCR4 antagonism was able to correct the abnormal frequencies and numbers of splenic marginal zone and follicular B cells in Cxcr4+/1013 mice, and ultimately normalize B-cell lymphopenia in the peripheral circulation.ConclusionsOur study provides comprehensive evidence that oral dosing with a CXCR4 antagonist can effectively correct WHIM-associated neutrophil and lymphocyte abnormalities in a mouse model of WHIM syndrome. These findings extend our understanding of how targeting the dysregulated CXCR4 signaling pathway can ameliorate the pathogenesis of WHIM syndrome.
Current hotspots and trends in cancer metabolic reprogramming: a scientometric analysis
Authors: Yang Shanshan , Lin Miaomiao , Hao Shaodong , Ye Hui , Zhang Xuezhi 
Published: November 2024

BackgroundMetabolic reprogramming (MR) in cancer (CA) has been a focus of intense research in the recent two decades. This phenomenon has attracted great interest because it offers potential targets for cancer therapy. To capture the intellectual landscape of this field, we conducted a bibliometric analysis to assess the scientific output, major contributors, and trends in the MR/CA research.MethodsWe performed a systematic search using the Web of Science to retrieve articles published on MR of cancer from 2006 until 2023. The bibliometric tools such as Biblioshiny, VOSviewer, and Microsoft Excel were used to identify the most prolific authors, institutions, citation patterns, and keywords. We also used co-citation analysis to map the conceptual structure of the field and identify influential publications. Furthermore, we examined the literature by analyzing publication years, citations, and research impact factors.ResultsA total of 4,465 publications about MR/CA were retrieved. Publications on MR/CA increased rapidly from 2006 to 2023. Frontiers in Oncology published the most papers, while Cell Metabolism had the most citations. Highly cited papers were mainly published in Cancer Cell, Nature, Cell, Science and Cell Metabolism. China and the United States led the way in publications and contributed the most to MR/CA research. The University of Texas System, Chinese Academy of Sciences, and Fudan University were the most productive institutions. The profitable authors were Deberardinis Ralph J and Chiarugi Paola. The current topics included MR in tumorigenesis and progression of CA, MR of tumor cells and tumor microenvironment, the effect of MR on the CA treatment, the underlying mechanisms of MR (such as gene regulation, epigenetics, extracellular vesicles, and gut microbiota), and the modulation of MR. Some topics such as tumor microenvironment, lipid MR, circular RNA, long noncoding RNA, exosome, prognostic model, and immunotherapy may be the focus of MR/CA research in the next few years.ConclusionThis study evaluated the global scientific output in the field of MR/CA research, analyzing its quantitative characteristics. It identified some significant and distinguished papers and compiled information regarding the current status and evolving trends of MR/CA research.