bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phosphate amendment drives bloom of RNA viruses after soil wet-up
Authors: Sieradzki Ella T. , Allen G. Michael , Kimbrel Jeffrey A. , Nicol Graeme W. , Hazard Christina , Nuccio Erin , Blazewicz Steven J. , Pett-Ridge Jennifer , Trubl Gareth 
Published: October 2024

AbstractSoil rewetting after a dry period results in a surge of activity and succession in both microbial and DNA virus communities. Less is known about the response of RNA viruses to soil rewetting—while they are highly diverse and widely distributed in soil, they remain understudied. We hypothesized that RNA viruses would show temporal succession following rewetting and that phosphate amendment would influence their trajectory, as viral proliferation may cause phosphorus limitation. Using 39 time-resolved metatranscriptomes and amplicon data, 2,190 RNA viral populations were identified across five phyla, with 37% of these predicted to infect bacteria (26%) or fungi (11%). Only 1.2% of viral populations had annotated capsid genes, suggesting most persist via intracellular replication without a free virion phase. Phosphate amendment altered RNA viral community composition within the first week and amended vs. unamended communities remained distinguishable for up to three weeks. While the overall host community remained stable, certain bacterial populations showed reduced abundance in phosphate-amended soils, likely due to increased viral lysis, as RNA bacteriophages, particularlyLeviviricetes, proliferated significantly. Notably, 60% of the viruses with increased abundance under phosphate amendment belonged to basalLenarviricotaclades rather than well-known groups likeLeviviricetes. We estimate RNA bacteriophage infections may affect 107–109bacteria per gram of soil, aligning with the total bacterial population (107–1010g−1soil), suggesting that RNA phages significantly influence bacterial communities post-wet-up, with phosphorus availability modulating this effect.HighlightsSoil wet-up influences RNA virus dynamics for over a weekThe majority of hosts predicted for RNA viruses were bacteria and fungiPhosphate amendment preferentially supportsLenarviricotaover other RNA viruses60% of vOTUs that responded to phosphate were from unknown bacteriophage cladesMost soil bacteria are predicted to be infected by RNA bacteriophages within a week
Spatiotemporal transcriptomic analyses reveal molecular gradient patterning during development and the tonotopic organization along the cochlear axis
Authors: Yan Mengzhen , Zhang Penghui , Wang Yafan , Wang Haojie , Li Junhong , Guo Xiang , Zeng Xiangyao , Feng Zhili , Gao Yushan , Deng Fei , Zhu Bicheng , Deng Di , Ma Lu , Feng Yong , Wu Hua-Jun , Zhao Yu , Li Jun 
Published: October 2024

AbstractDisruption of cochlear architecture and development can lead to malfunction, resulting in hearing defects. However, the spatial molecular profiles critical for hearing function remain poorly understood due to the structural complexity of the cochlea. In this study, we performed comprehensive spatiotemporal transcriptomic analyses on the developing and adult cochlea, identifying numerous genes with gradient expression patterns in hair cells (HCs) and spiral ganglion neurons (SGNs) across the apical-to-basal axis. The gradient geneMyo7a, a well-known HC marker, established a decreasing gradient in outer hair cells (OHCs) from the apical to basal regions in adulthood. In contrast, the other class of gradient genes exhibited an opposing expression pattern toMyo7aalong the cochlear axis in adulthood, such asCalb2in HCs andNefhin SGNs, which potentially corresponds to the cochlear tuning properties for sensing gradient frequency sounds. Importantly, our analyses provided compelling evidences for the existence of distinct spatial subtypes of OHCs. The spatial analyses revealed regional heterogeneity in cell communication intensity between HCs and SGNs, with the weakest intensity at the apex, potentially aligning this region for detection of low-frequency sound. This study systematically characterized the fine spatial organizations in HCs, SGNs, and their regionalized cell-communications within the cochlea, offering insights into the morphological and molecular foundations underlying cochlear tonotopic organization and frequency discrimination.
Site-specific quantification of thein vivoUFMylome reveals myosin modification in ALS
Authors: Blazev Ronnie , Zee Barry M. , Peckham Hayley , Ng Yaan-Kit , Lewis Christopher T. A. , Zhang Chengxin , McNamara James W. , Goodman Craig A. , Gregorevic Paul , Ochala Julien , Steyn Frederik J. , Ngo Shyuan T. , Stokes Matthew P. , Parker Benjamin L. 
Published: October 2024

AbstractUFMylation is a ubiquitin-like protein post-translational modification of Ubiquitin Fold Modifier 1 (UFM1) applied to substrate proteins. The UFMylation system is important for normal development and plays a critical role in a variety of cellular processes including regulating telomere length, stress responses and protein quality control. Here, we describe the development of an antibody-based enrichment approach to immunoprecipitatein vivoremnant UFMylated peptides and identification by liquid chromatography and tandem mass spectrometry (LC-MS/MS). We used this approach to identify >200 UFMylation sites from various mouse tissues revealing extensive modification in skeletal muscle. Furthermore, we show that UFMylation is increased in skeletal muscle biopsies from people living with Amyotrophic Lateral Sclerosis (plwALS). Quantification of UFMylation sites in these participant biopsies with multiplexed isotopic labeling and LC-MS/MS reveal prominent increases in myosin UFMylation. Finally,in silicomodelling suggest UFMylation of myosin directly adjacent to the ATP-binding site may regulate stability and/or function. Our data suggest that although UFMylation is not as widespread as ubiquitylation, itsin vivostatus is more complex than previously thought.
Investigation on the stabilities of doubly heavy tetraquark states
Authors: Park Woosung , Noh Sungsik 
Published: October 2024

In our recent work [S. Noh and W. Park, Nonrelativistic quark model analysis of Tcc, ], the mass and binding energy of Tcc are found to be 3873 MeV and −2  MeV, respectively, which align with the observations reported at LHCb [R. Aaij (LHCb Collaboration), Observation of an exotic narrow doubly charmed tetraquark, ]. Based on our latest quark model approach, we extend our search for other potentially stable configurations of doubly heavy tetraquarks using our nonrelativistic quark model described in Noh and Park, [Nonrelativistic quark model analysis of Tcc, ]. Our numerical calculations indicate that the u¯s¯cb configuration is deeply bound. However, the u¯d¯cb configuration with the isospin symmetry in the light quark sector is relatively less bound. In this study, we emphasize a compulsory aspect for requiring a complete set of three-dimensional harmonic oscillator bases through the discussion of the u¯s¯cb configuration and investigate the essential differences between the u¯s¯cb and u¯d¯cb configurations.

Published by the American Physical Society
2024
Greening by enhancing self-efficacy: a viewpoint on employee-inclusive Octalysis framework
Authors: Haque Shamima , Panda Debadrita 
Published: October 2024

Purpose
The purpose of this study is to investigate how gamification can be utilized to promote Employee Green Behavior (EGB) within organizations thereby enhancing corporate sustainability. The research aims to propose a structured gamification framework that can effectively motivate and engage employees in sustainability initiatives.

Design/methodology/approach
The viewpoint reviews existing literature on gamification, and its impact on EGB. The authors propose a conceptual model that highlights critical components for Octalysis framework and map this against various player types for various phases of a gamified system.

Findings
The Octalysis framework, with its eight core drives, is found to be an effective tool for fostering EGB by leveraging both intrinsic and extrinsic motivations. The viewpoint demonstrates that categorizing employees into distinct player types and tailoring gamification strategies accordingly can overcome motivational barriers and encourage sustained engagement in green practices.

Originality/value
This study introduces a novel approach to corporate sustainability by integrating gamification into EGB initiatives. This innovative approach enhances the understanding of EGB and provides practical insights for implementing effective gamification strategies in corporate sustainability efforts.
Intangible cultural heritage transmission: empowering generations through Emirati handicrafts at Turath center
Authors: Kottangal Achuthy , Purohit Deepika 
Published: October 2024

PurposeIn order to preserve and honor authentic Bedouin Emirati heritage history, the Turath Centre for Traditional Handicrafts, which operates under the supervision of the Art authority of Dubai culture, which is essential. The center aims and its strategy for cultural preservation, then the effects of its operations on community development are all explored in this article.Design/methodology/approachThe goals and strategies employed by the Turath Centre to promote traditional Emirati crafts are analyzed in this study using the qualitative methodology that blends historical analysis and ethnographical research. It also examines the effects of the center's training programs and workshops. This study reveals the methods the Turath Centre uses to disseminate cultural heritage through a thorough analysis of the supporting documentation and on-site observations.FindingsAccording to the research, the Turath Center effectively instructs community people in a variety of craft areas, such as traditional weaving, silversmith work, and burqa sewing and intangible facets of Emirati history though Al Talli and Al Sadu Weave. This study highlights the value of the Turath Centre's contribution to community development and preservation of Emirati heritage though younger generations.Research limitations/implicationsAlthough this study offers insightful information, there are certain limitations. Specifically, more research is required to evaluate the efforts of the Turath Center's adaptability and impact over time.Practical implicationsThe results highlighted the consideration to these educational programs and their capacity to foster heritage industries, increase cultural tourism, and promote Emirati culture internationally.Social implicationsBy enhancing Bedouin heritage culture and encouraging pride and identity among community members, the Turath Center's activities support unity in society.Originality/valueThis research offers valuable insights into the Turath Centre's role in preserving Bedouin Emirati heritage and fostering community development, the center symbolizes Dubai's aim for cultural growth and makes a significant contribution to cultural preservation.
Multisensory healing: transformative service encounters in nonpharmacological therapies for cancer patients’ well-being
Authors: Rezaee Vessal Saeedeh , El Euch Maalej Mariem , Partouche-Sebban Judith , Toledano Alain 
Published: October 2024

Purpose
This study aims to explore the impact of nonpharmacological therapies on cancer patients’ daily illness management and long-term well-being. It focuses on the design and effects of immersive multisensory workshops, including therapies such as yoga, music therapy and visual imagery. By examining individuals’ experiences before, during and after the service experience, the study aims to provide comprehensive insights into the transformative effects of these immersive multisensorial experiences from the individuals’ perspectives.

Design/methodology/approach
From November 2021 to March 2023, the authors conducted 13 interviews and 3 focus groups (10 participants in total) among cancer patients. Thematic analysis of recorded interviews and focus groups revealed recurring patterns, key themes and meaningful insights from participants’ narratives.

Findings
The findings provide insights into individuals’ journey of service experiences among cancer patients from a user perspective. By framing the results within the service encounters model, individuals’ journey of this immersive multisensory experience is studied in three different periods: the pre-core, the core and the post-core service encounter. The pre-core service encounter includes activities such as information gathering to answer concerns and setting expectations, with the therapy. The core service encounter encompasses the immersive environmental experience, which includes multisensory integration and activity immersion, body-mind reconnection, as well as engaging interactive experiences with service providers, other patients and internal engagement. The post-core service encounter reflects the cognitive, psychological, behavioral and spiritual outcomes of the service.

Originality/value
This paper sheds light on the design of immersive multisensory workshops as nonpharmacological therapy. Adopting a user-focused approach using the service encounters framework helps clarify various aspects of this therapy and its effects on patients’ reconnection with their bodies and well-being. This research offers valuable insights for designing effective multisensory therapeutic environments for chronic patients to improve the quality of health-care services.
Arginine metabolism is a biomarker of red blood cell and human aging
Authors: Reisz Julie A. , Earley Eric J. , Nemkov Travis , Key Alicia , Stephenson Daniel , Keele Gregory R. , Dzieciatkowska Monika , Spitalnik Steven L. , Hod Eldad A. , Kleinman Steven , Roubinian Nareg H. , Gladwin Mark T. , Hansen Kirk C. , Norris Philip J. , Busch Michael P. , Zimring James C. , Churchill Gary A. , Page Grier P. , D'Alessandro Angelo 
Published: October 2024

AbstractIncreasing global life expectancy motivates investigations of molecular mechanisms of aging and age‐related diseases. This study examines age‐associated changes in red blood cells (RBCs), the most numerous host cell in humans. Four cohorts, including healthy individuals and patients with sickle cell disease, were analyzed to define age‐dependent changes in RBC metabolism. Over 15,700 specimens from 13,757 humans were examined, a major expansion over previous studies of RBCs in aging. Multi‐omics approaches identified chronological age‐related alterations in the arginine pathway with increased arginine utilization in RBCs from older individuals. These changes were consistent across healthy and sickle cell disease cohorts and were influenced by genetic variation, sex, and body mass index. Integrating multi‐omics data and metabolite quantitative trait loci (mQTL) in humans and 525 diversity outbred mice functionally linked metabolism of arginine during RBC storage to increased vesiculation—a hallmark of RBC aging—and lower post‐transfusion hemoglobin increments. Thus, arginine metabolism is a biomarker of RBC and organismal aging, suggesting potential new targets for addressing sequelae of aging.
Neurological dysfunction and fatal encephalitis due to Halicephalobus gingivalis in two horses in Belgium
Authors: Calewaert A. , Dufourni A. , Stee K. , Hiltrop R. , Raes E. , van Loon G. 
Published: October 2024

SummaryTwo horses kept on different farms at a geographical distance of 150 km were presented with acute neurological dysfunction. Ante‐mortem diagnostic tests including bloodwork, CT scan and cerebrospinal fluid analysis did not provide a diagnosis in either case. Due to rapid deterioration, both horses were euthanised shortly after admission. Postmortem histopathological examination revealed granulomatous meningoencephalitis with intralesional nematodes that were morphologically identified as Halicephalobus gingivalis. This is the first documentation of central nervous system infestation by H. gingivalis in horses residing in Belgium, emphasising the importance of considering aberrant parasitic migration in the differential diagnosis of acute neurological dysfunction.
Hypothalamic–pituitary–adrenal axis plasticity across life‐history stages of a free‐living subtropical finch, Amandava amandava amandava
Authors: Mohanty Banalata 
Published: October 2024

AbstractThe alterations of phenotypic traits (morphology, endocrine physiology, and behavior) in response to predictable environmental cues across life‐history stages in seasonally breeding birds enable successful culmination of reproduction. The present study elucidated the plasticity of the hypothalamic–pituitary–adrenal (HPA) axis in a subtropical free‐living finch, Amandava amandava amandava, and suggests the crucial role of the baseline corticosterone (CORT) to coordinate energetic readiness across life‐history stages. Birds were captured monthly from an area (25.1337° N 82.5644° E) in Uttar Pradesh, India, from June 2014 to May 2015. Only male birds were included in this study corresponding to different life‐history stages (6/life‐history stage; 2/month): pre‐breeding (June–August), breeding (September–November), post‐breeding (December–February), and quiescent phases (March–May). The pituitary expression of adrenocorticotropic hormone (ACTH), adrenal interrenal cell morphometry, and plasma level of the CORT showed varied patterns across life‐history stages. The density and immunointensity of the ACTH‐immunoreactive corticotropes and the interrenal cell number increased along with the significant plasma CORT elevation during the breeding cycle (both pre‐breeding and breeding phases). CORT might facilitate the energy demand for the display of sexual behavior (nest‐building, courtship), testicular recrudescence, and foraging of food for offspring during the breeding cycle. On the contrary, plasma CORT decrease in the post‐breeding and quiescent phases might enable the bird to molt avoiding the protein catabolic effect of the hormone. Given the complexity involved in the study of baseline CORT in free‐living birds, more studies are needed to better understand the crucial role of the HPA axis in the modulation of life‐history stages in this and other subtropical avian species.
Menstrual blood‐derived mesenchymal stem cells combining with platelet‐rich plasma infusion in endometrium repair
Authors: Wang Yang , Liu Wenxin , Xu Hui , Dong Liyuan , Jiao Yalou , Qiao Zhe 
Published: October 2024

AbstractObjectiveThin endometrium caused by various factors affects the conception rate of females worldwide; however, current medications are still insufficient. Therefore, a novel approach is needed. We previously reported the effect of menstrual blood‐derived mesenchymal stem cells (MenSCs) in ameliorating ethanol‐induced endometrial injuries. In the present study, we aimed to investigate whether the effect of MenSCs is enhanced by a combination of platelet‐rich plasma (PRP) infusion.MethodsAn endometrial injury mouse model was established by infiltrating 95% ethanol for 15 s into the uterus, followed by MenSCs, PRP, or MenSCs + PRP treatment. Pathological changes were observed by HE staining. The expression of CK18, vimentin, ItgαVβ3, and VEGF was determined using IHC staining and WB blotting.ResultsCompared with the model, MenSCs, PRP, and MenSCs + PRP treatments significantly improved endometrial damage and thickness, with the combined therapy displaying the most pronounced efficacy. The density of CK18‐, vimentin‐, and ItgαVβ3 positive cells increased most significantly in the MenSCs + PRP group of mice. In addition, the protein expression of CK18, vimentin, and VEGF was significantly upregulated after MenSCs + PRP, MenSCs, and PRP treatment, with MenSCs + PRP therapy showing the best efficacy.ConclusionMenSCs + PRP therapy is more beneficial for ameliorating ethanol‐induced endometrial damage than MenSCs or PRP alone, providing a basis for the investigation of novel approaches for treating thin endometria.
Clozapine and objective assessment of hypersomnolence in patients with schizophrenia: a systematic review
Authors: Quiles Clélia , Taillard Jacques , Lopez Régis , Geoffroy Pierre Alexis , Salvo Francesco , Micoulaud‐Franchi Jean‐Arthur 
Published: October 2024

SummaryClozapine is effective in treatment‐resistant schizophrenia but with adverse effects including sedation. Excessive daytime sleepiness, a symptom of hypersomnolence, is the most frequently reported subjective side‐effect. The aim of this systematic review was to synthesise the literature evaluating the impact of clozapine on the objective assessment of hypersomnolence in people with schizophrenia. We systematically searched databases for articles evaluating hypersomnolence with electrophysiological or psychomotor/cognitive measures in clozapine‐treated patients with schizophrenia. Objective assessment of hypersomnolence was evaluated in six studies. All studies using polysomnography (PSG) found significantly longer total sleep time and shorter sleep onset latency in patients treated with clozapine at initiation of clozapine. The study with the multiple sleep latency test (MSLT) also found a shorter sleep onset latency. These observations did not persist 4–6 weeks after treatment initiation. Further investigations are needed. Longer total sleep time should be investigated with standardised long‐term PSG to investigate excessive sleep quantity. Shorter sleep onset latency should be investigated with the MSLT or the maintenance of wakefulness test to investigate the excessive propensity to fall asleep or ability to stay awake. Lastly, sleep inertia should be investigated specifically in the morning.
Do pregnant women with restless legs syndrome experience better sleep with physical activity?
Authors: Esteves Andrea Maculano , Hackethal Sandra , Riccardi Silvia , Garbazza Corrado , Manconi Mauro 
Published: October 2024

SummaryPregnant women benefit greatly from exercise due to its powerful impact on maternal and perinatal outcomes. The purpose of our study was to assess whether exercise improves objective sleep quality in pregnant women suffering from restless legs syndrome. This cross‐sectional study is based on data from a large, naturalistic, consecutive cohort study in pregnant women recruited by four sleep centres, in Bologna, Milan and Turin (Italy), and Lugano (Switzerland), named the Life‐ON study. Objective sleep parameters of 316 pregnant women were measured using polysomnographic recordings during the second trimester of pregnancy, and a self‐reported assessment was used to evaluate physical activity. Pregnant women with restless legs syndrome (n = 91) who engaged in physical activity (n = 28) experienced better sleep efficiency (84.20% versus 82.10%, p = 0.01), less stage 1 sleep (10.50% versus 11.40%, p = 0.04) and wake after sleep onset (52.20 min versus 76.40 min, p ≤ 0.001), as well as reduced periodic leg movements during sleep (5.50 per hr versus 16.40 per hr, p < 0.001) when compared with the sedentary restless legs syndrome group (n = 63). Sleep structure was also better conserved in physically active pregnant women without restless legs syndrome compared with the inactive restless legs syndrome negative group. Overall, this study provides further evidence of the benefits of physical activity in pregnant women by illustrating its positive impact on sleep structure.