bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of key lncRNAs in the carcinogenesis and progression of colon adenocarcinoma by co‐expression network analysis
Authors: Jiang Shi , Tan Biyong , Zhang Xingqiang 
Published: November 2018

AbstractColon adenocarcinoma (COAD) is one of the most common cancers, and its carcinogenesis and progression is influenced by multiple long non‐coding RNAs (lncRNA), especially through the miRNA sponge effect. In this study, more than 4000 lncRNAs were re‐annotated from the microarray datasets through probe sequence mapping to obtain reliable lncRNA expression profiles. As a systems biology method for describing the correlation patterns among genes across microarray samples, weighted gene co‐expression network analysis was conducted to identify lncRNA modules associated with the five stepwise stages from normal colonic samples to COAD (n = 94). In the most relevant module (R2 = −0.78, P = 4E‐20), four hub lncRNAs were identified (CTD‐2396E7.11, PCGF5, RP11‐33O4.1, and RP11‐164P12.5). Then, these four hub lncRNAs were validated using two other independent datasets including GSE20916 (n = 145) and GSE39582 (n = 552). The results indicated that all hub lncRNAs were significantly negatively correlated with the three‐stage colonic carcinogenesis, as well as TNM stages in COAD (one‐way analysis of variance P < 0.05). Kaplan‐Meier survival curve showed that patients with higher expression of each hub lncRNA had a significantly higher overall survival rate and lower relapse risk (log‐rank P < 0.05). In conclusion, through co‐expression analysis, we identified and validated four key lncRNAs in association with the carcinogenesis and progression of COAD, and these lncRNAs might have important clinical implications for improving the risk stratification, therapeutic decision and prognosis prediction in COAD patients.
The Alliance and Rupture Observation Scale (AROS): Development and validation of an alliance and rupture measure for repeated observations within psychotherapy sessions
Authors: Larsson Mattias Holmqvist , Björkman Karin , Nilsson Karin , Falkenström Fredrik , Holmqvist Rolf 
Published: November 2018

AbstractObjectiveThe aim of this study was to test a new observer‐rated instrument, the Alliance and Rupture Observation Scale (AROS). It was designed for repeated measurements of the alliance within sessions and to detect alliance ruptures.MethodVideotaped therapy sessions with depressed adults were analyzed. Reliability was mainly assessed as inter‐rater reliability. Convergent, predictive, and discriminant validity of the AROS was assessed by comparing the instrument with both observer‐rated and patient‐rated measures.ResultsThe AROS exhibited excellent inter‐rater reliability. Alliance levels measured with the AROS predicted patients’ ratings of the alliance in the same session and were highly correlated with another observer‐rated alliance measure. Alliance patterns (rupture; repair; and no‐rupture) based on AROS scores were significantly correlated with patients’ ratings of the alliance.ConclusionsPreliminary support for convergent and predictive validity was found. It is yet to be determined whether AROS scores are related to psychotherapy outcomes.
A qualitative analysis of emotion and emotion regulation in hoarding disorder
Authors: Taylor Jasmine K. , Theiler Stephen , Nedeljkovic Maja , Moulding Richard 
Published: November 2018

AbstractObjectiveThe role of emotion regulation (ER) has been receiving increased attention in relation to various forms of psychopathology including hoarding disorder (HD). However, questionnaire designs are limited to finding associations of ER with symptoms or symptom groups, without finding out how such constructs might be involved in the disorder.MethodsThis study was a qualitative investigation of ER in a clinical HD sample (N = 11).ResultsProminent themes provided support for ER difficulties in hoarding. In particular, difficulties with identifying and describing feelings, unhelpful attitudes toward the emotional experience, the use of avoidance‐based strategies, and a perceived lack of effective ER strategies were prominent themes. Furthermore, emotional factors were identified as being associated with the onset and/or exacerbation of hoarding behavior, and possessions and acquiring behavior appeared to serve an ER function.ConclusionThe current paper provides a nuanced account of the role of ER in hoarding difficulties.
High‐speed quantitative 3D imaging by dual‐illumination holographic microscopy
Authors: Donnarumma Dario , Rawat Nitin , Brodoline Alexey 
Published: November 2018

AbstractA new blood flow imaging (BFI) technique using digital holography with double illumination of the sample is proposed. We imaged the moving red blood cells (RBCs) using a two microscope objective lenses setup. The setup consists in a larger angle of separation (90 °) between the two illumination beams, allowing a wider angular rotation at good z resolution. Moreover, the setup geometry allows an easier displacement of the sample in all directions. Results show that this technique is able to perform phase‐shifting reconstruction for the two beams at the same time which is more suitable for the future implementation of live 3D holography. Experimental results are carried out for the verification of the effectiveness of the proposed technique on a zebrafish larvae sample.Research highlights

Blood flow imaging techniques are often invasive and image analysis is time consuming.
To alleviate this issue an imaging technique based on dual illumination in holographic domain is proposed.
This method has been validated on zebrafish embryos.
A Triple‐Classification Radiomics Model for the Differentiation of Primary Chordoma, Giant Cell Tumor, and Metastatic Tumor of Sacrum Based on T2‐Weighted and Contrast‐Enhanced T1‐Weighted MRI
Authors: Yin Ping , Mao Ning , Zhao Chao , Wu Jiangfen , Chen Lei , Hong Nan 
Published: November 2018

BackgroundPreoperative differentiation between primary sacral chordoma (SC), sacral giant cell tumor (SGCT), and sacral metastatic tumor (SMT) is important for treatment decisions.PurposeTo develop and validate a triple‐classification radiomics model for the preoperative differentiation of SC, SGCT, and SMT based on T2‐weighted fat saturation (T2w FS) and contrast‐enhanced T1‐weighted (CE T1w) MRI.Study typeRetrospective.PopulationA total of 120 pathologically confirmed sacral patients (54 SCs, 30 SGCTs, and 36 SMTs) were retrospectively analyzed and divided into a training set (n = 83) and a validation set (n = 37).Field strength/sequenceThe 3.0T axial T2w FS and CE T1w MRI.AssessmentMorphology, intensity, and texture features were assessed based on Formfactor, Haralick, Gray‐level co‐occurrence matrix (GLCM), Gray‐level run‐length matrix (GLRLM), histogram.Statistical testsAnalysis of variance, least absolute shrinkage and selection operator (LASSO), Pearson correlation, Random Forest (RF), area under the receiver operating characteristic curve (AUC) and accuracy analysis.ResultsThe median age of SGCT (33.5, 25.3–45.5) was significantly lower than those of SC (58.0, 48.8–64.3) and SMT (59.0, 46.3–65.5) groups (χ2 = 37.6; P < 0.05). No significant difference was found when compared in terms of genders, tumor locations, and tumor sizes of SC, SGCT, and SMT (; P1 = 0.15, P2 = 0.29, P3 = 0.06). For the differential value, features extracted from joint T2w FS and CE T1w images outperformed those from T2w FS or CE T1w images alone. Compared with CE T1w images, features derived from T2w FS images yielded higher AUC in both training and validating set. The best performance of radiomics model based on joint T2w FS and CE T1w images reached an AUC of 0.773, an accuracy of 0.711.Data conclusionOur 3.0T MRI‐based triple‐classification radiomics model is feasible to differentiate SC, SGCT, and SMT, which may be applied to improve the precision of preoperative diagnosis in clinical practice.Level of Evidence: 4Technical Efficacy: Stage 2J. Magn. Reson. Imaging 2019;49:752–759.
Characterization of clinical human prostate cancer lesions using 3.0‐T sodium MRI registered to Gleason‐graded whole‐mount histopathology
Authors: Broeke Nolan C. , Peterson Justin , Lee Joseph , Martin Peter R. , Farag Adam , Gomez Jose A. , Moussa Madeleine , Gaed Mena , Chin Joseph , Pautler Stephen E. , Ward Aaron , Bauman Glenn , Bartha Robert , Scholl Timothy J. 
Published: November 2018

BackgroundOvertreatment of prostate cancer (PCa) is a healthcare issue. Development of noninvasive imaging tools for improved characterization of prostate lesions might reduce overtreatment.PurposeTo measure the distribution of tissue sodium concentration (TSC), proton T2‐weighted signal, and apparent diffusion coefficient (ADC) values in human PCa and to test the presence of a correlation between regional differences in imaging metrics and the Gleason grade of lesions determined from histopathology.Study TypeCross‐sectional.SubjectsTen men with biopsy‐proven PCa.Sequences/Field StrengthSodium, proton T2‐weighted, and diffusion‐weighted MRI data were acquired using Broad‐Band 3D‐Fast‐Gradient‐Recalled, 3D Cube (Isotropic 3D‐Fast‐Turbo‐Spin‐Echo acquisition) and 2D Spin‐Echo sequences, respectively, with a 3.0T MR scanner.AssessmentAll imaging data were coregistered to Gleason‐graded postprostatectomy histology, as the standard for prostate cancer lesion characterization. Regional TSC and T2 data were assessed using percent changes from healthy tissue of the same patient (denoted ΔTSC, ΔT2).StatisticsDifferences in ΔTSC, ADC, and ΔT2 as a function of Gleason score were analyzed for each imaging contrast using a one‐way analysis of variance or a nonparametric t‐test. Correlations between imaging data measures and Gleason score were assessed using a Spearman's ranked correlation.ResultsEvaluation of the correlation of ΔTSC, ADC, and ΔT2 datasets with Gleason scoring revealed that only the correlation between ΔTSC and Gleason score was statistically significant (rs = 0.791, p < 0.01), whereas the correlations of ADC and ΔT2 with Gleason score were not (rs = –0.306, p = 0.079 and r
s = –0.069, p = 0.699, respectively). In addition, all individual patients showed monotonically increasing ΔTSC with Gleason score.Data ConclusionThe results of this preliminary study suggest that changes in TSC, assessed by sodium MRI, has utility as a noninvasive imaging assay to accurately characterize PCa lesions. Sodium MRI may provide useful complementary information on mpMRI, which may assist the decision‐making of men choosing either active surveillance or treatment.Level of Evidence: 1Technical Efficacy: Stage 2J. Magn. Reson. Imaging 2019;49:1409–1419.
Amide proton transfer imaging to evaluate the grading of squamous cell carcinoma of the cervix: A comparative study using 18F FDG PET
Authors: Li Beibei , Sun Hongzan , Zhang Siyu , Wang Xiaoqi , Guo Qiyong 
Published: November 2018

BackgroundAmide proton transfer (APT) imaging has shown great potential value in the diagnosis of cancer, but has yet not been applied in cervical carcinoma patients.PurposeTo investigate the utility of APT imaging in estimating histologic grades of squamous cell carcinoma of the cervix (SCCC), compared with the standardized uptake value (SUV).Study TypeProspective.PopulationThirty‐one patients with SCCC (median age 51 years) were included.Field Strength/SequenceIngenia 3.0 T CX, Axial T1‐weighted imaging (T1WI), Axial T2WI, 3D turbo spin echo sequence for APT imaging.AssessmentPatient pathology was confirmed by surgery and the patients were divided into three groups based on histologic grades: Grade 1 (n = 9), Grade 2 (n = 12), and Grade 3 (n = 10). The APT signal intensity (APT SI), maximum SUV (SUVmax) and mean SUV (SUVmean) for each grade were assessed by experienced radiologists in a blinded manner.Statistical TestsThe obtained parameters were compared by one‐way analysis of variance with Tukey honest significant difference post‐hoc test. The correlations between the parameters and histologic grades were analyzed by Spearman's correlation coefficient. The Pearson correlation coefficients of the APT SI with the SUVmax and SUVmean were also calculated.ResultsThe APT SIs for the three grades were significantly different (P = 0.0002). The APT SIs of Grade 2 and Grade 3 had significant differences (P = 0.009). The Spearman correlation coefficients for the correlations between the parameters and histological grade were as follows: APT SI: 0.684 (P = 0.00002), SUVmax: 0.318 (P = 0.082), and SUVmean: 0.261 (P = 0.157). The Pearson correlation coefficients of the APT SI with the SUVmax and SUVmean were 0.108 (P = 0.564) and 0.178 (P = 0.337), respectively.Data ConclusionThe APT SI was positively correlated with the SCCC grades. APT imaging maybe a promising method for predicting SCCC histologic grades.Level of Evidence: 2Technical Efficacy: Stage 2J. Magn. Reson. Imaging 2019;50:261–268.
Duration of postoperative immobilization affects MMP activity at the healing graft–bone interface: Evaluation in a mouse ACL reconstruction model
Authors: Nakagawa Yusuke , Lebaschi Amir H. , Wada Susumu , Green Samuel J. E. , Wang Dean , Album Zoe M. , Carballo Camilla B. , Deng Xiang‐Hua , Rodeo Scott A. 
Published: November 2018

ABSTRACTExcessive MMP activity may impair tendon‐to‐bone healing. However, little is known about the effect of joint motion on MMP activity after ACL reconstruction. The aim of this study was to determine the effect of different durations of knee immobilization on MMP activity in a mouse ACL reconstruction model using a fluorescent MMP probe which detects MMP 2, 3, 9, and 13 and near‐infra red in vivo imaging. Sixty C57BL male mice underwent ACL reconstruction. Post‐operatively, the animals were treated with free cage activity (Group 1), or with the use of an external fixator to restrict knee motion and weight bearing for 5 days (Group 2), 14 days (Group 3), and 28 days (Group 4). At days 3, 7, 16, 23, and 30, five mice underwent IVIS imaging. At days 3, 7, 16, and 30, histological analysis was also performed. Probe signal intensity in the whole limb peaked at day 7, followed by a decrease at day 16, and maintenance up to day 30. There was no significant difference among groups at any time point based on IVIS, but histologic localization of MMP probe signal showed significantly less activity in Group 2 and Group 3 compared to Group 4 in the bone tunnel at day 30. We demonstrated that short‐term immobilization led to less MMP activity around the bone tunnel compared with prolonged immobilization. A short period of immobilization after ACL reconstruction might enhance graft–bone interface healing by mitigating excess MMP expression. These findings have implications for post‐operative rehabilitation protocols following ACL reconstruction. © 2018 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 37:325–334, 2019.
Occurrence and seasonality of aflatoxin M1 in milk in two different climate zones
Authors: Venâncio Rafael Luiz , Ludovico Agostinho , de Santana Elsa Helena Walter , de Toledo Eduardo Amaral , de Almeida Rego Fabiola Cristine , dos Santos Joice Sifuentes 
Published: November 2018

AbstractBACKGROUNDAflatoxin M1 (AFM1) is a mycotoxin found in milk as a result of the ingestion of aflatoxin B1 (AFB1) by dairy cattle. Because of its carcinogenic properties, the control of its occurrence in dairy products is of great importance. We evaluated the occurrence and seasonality of AFM1 in milk from farms with subtropical and temperate climates, where significant milk production occurs. Forty samples of raw milk were collected from bulk tansk milk during the summer (five samples from a subtropical climate and 14 from a temperate climate) and winter (six samples from a subtropical climate and 15 from a temperate climate) months of 2017 and conducted an analysis using an enzyme‐linked immunosorbent assay (ELISA) (detection limit 5 ng L−1). Data from eight random samples were compared with respect to liquid chromatography with mass spectrometry detection and ELISA. A significant correlation was observed.RESULTSThe presence of AFM1 was detected in 87.50% (n = 35) of the samples analyzed (a mean of 16.66 ng L−1 and a median of 12.42 ng L−1 in positive samples); however, its concentration in all samples was below the maximum limit allowed by European (50 ng L−1) and Brazilian (500 ng L−1) legislations. There were no significant differences in the levels of AFM1 between the properties located in the two climate zones, in both summer and winter. The estimated daily intake of AFM1 based on the milk analyzed was 0.0107 ng kg−1 day−1 for adolescents, 0.0072 ng kg−1 day−1 for adults and 0.0098 ng kg−1 day−1 for the elderly.CONCLUSIONThe present work demonstrated a low exposure to AFM1. © 2018 Society of Chemical Industry
Fungal β‐1,3‐1,4‐glucanases: production, proprieties and biotechnological applications
Authors: Chaari Fatma , Chaabouni Semia Ellouz 
Published: November 2018

Abstractβ‐1,3‐1,4‐glucanases (or lichenases; EC 3.2.1.73) comprise one of the main enzymes used in industry during recent decades. These enzymes hydrolyze β‐glucans containing β‐1,3 and β‐1,4 linkages, such as cereal β‐glucans and lichenan. The β‐1,3‐1,4‐glucanases are produced by a variety of bacteria, fungi, plants and animals. A large number of microbial β‐1,3‐1,4‐glucanases have potential application in industrial processes, such as feed, food and detergent industries. The present review summarizes the available studies with respect to β‐1,3‐1,4‐glucanases production conditions, enzyme biochemical properties and potential industrial application. © 2018 Society of Chemical Industry
A novel colorimetric immunosensor based on platinum colloid nanoparticles immobilized on PowerVision as signal probes and Fe3O4@β‐cyclodextrin as capture probes for ractopamine detection in pork
Authors: Sun Yangying , Fu Tian , Chen Shuxian , Wu Zhen , Guo Yuxing , Pan Daodong , Gan Ning 
Published: November 2018

AbstractBackgroundA novel colorimetric immunosensor was developed for the simple, sensitive and selective detection of ractopamine (RAC) based on using β‐cyclodextrin‐modified Fe3O4 particles (Fe3O4@β‐CD) as capture probes and complex platinum colloid nanoparticles (PtNPs‐PV) composed of platinum colloid nanoparticles (PtNPs) and polymerase chelate PowerVision (PV) as signal probes.ResultsPtNPs‐PV double catalyzed the chromogenic substrate 3,3′‐diaminobenzidine (DAB), which induced changes in the color of DAB and chromogenic absorbance. Incubation temperature, pH and incubation time were systematically optimized and, under optimum conditions, the measured absorbance values showed a linear relationship with the RAC concentrations in the range 0.03–8.1 ng mL−1. The detection limit was 0.01 ng mL−1. The sensor exhibited high sensitivity and specificity, as demonstrated by testing structurally similar organic compounds such as salbutamol, clenbuterol and dopamine. The practicality of the developed colorimetric immunosensor was supported by the successful detection of RAC in pork samples with recovery ranging from 94.00% to 106.00%.ConclusionWe designed a novel sandwich‐type noncompetitive colorimetric immunoassay for the detection of trace levels of RAC in pork. The proposed method can also be used for the detection of toxins in food products via PtNPs‐PV amplification. © 2018 Society of Chemical Industry
Differential Associations Among PTSD and Complex PTSD Symptoms and Traumatic Experiences and Postmigration Difficulties in a Culturally Diverse Refugee Sample
Authors: Hecker Tobias , Huber Stephanie , Maier Thomas , Maercker Andreas 
Published: November 2018

AbstractForced migration is one of the major challenges currently facing the international community. Many refugees have been affected by traumatic experiences at home and during their flight, putting them at a heightened risk of developing trauma‐related disorders. The new version of the International Classification of Diseases (ICD‐11) introduced two sibling disorders, posttraumatic stress disorder (PTSD) and complex PTSD (CPTSD). So far, little is known about risk and protective factors in refugees that are specifically associated with the disturbances in self‐organization (DSO) characteristic of CPTSD. In this study, we aimed to investigate the association between PTSD and DSO symptoms and traumatic experiences, postmigration difficulties, and social support in a culturally diverse sample of refugees who resettled in Switzerland. A total of 94 refugees (85.1% male; M age = 31.60 years, SD = 10.14, range: 18–61 years) participated in this study. Trained assessors performed either guided questionnaire assessments or structured interviews. In our advice‐ and help‐seeking sample, 32.9% of individuals suffered from PTSD and 21.3% from CPTSD. After controlling for potential gender differences, we found positive associations between PTSD symptoms and trauma exposure, β = .22, as well as between DSO symptoms and postmigration living difficulties, β = .42, and lack of social support, β = .22. Our findings support the notion that it is highly important to consider differential associations among PTSD and DSO symptoms and risk and protective factors to gain a deeper understanding of the trauma‐related problems refugees face.
3D gradient echo snapshot CEST MRI with low power saturation for human studies at 3T
Authors: Deshmane Anagha , Zaiss Moritz , Lindig Tobias , Herz Kai , Schuppert Mark , Gandhi Chirayu , Bender Benjamin , Ernemann Ulrike , Scheffler Klaus 
Published: November 2018

PurposeFor clinical implementation, a chemical exchange saturation transfer (CEST) imaging sequence must be fast, with high signal‐to‐noise ratio (SNR), 3D coverage, and produce robust contrast. However, spectrally selective CEST contrast requires dense sampling of the Z‐spectrum, which increases scan duration. This article proposes a compromise: using a 3D snapshot gradient echo (GRE) readout with optimized CEST presaturation, sampling, and postprocessing, highly resolved Z‐spectroscopy at 3T is made possible with 3D coverage at almost no extra time cost.MethodsA 3D snapshot CEST sequence was optimized for low‐power CEST MRI at 3T. Pulsed saturation was optimized for saturation power and saturation duration. Spectral sampling and postprocessing (B0correction, denoising) was optimized for spectrally selective Lorentzian CEST effect extraction. Reproducibility was demonstrated in 3 healthy volunteers and feasibility was shown in 1 tumor patient.ResultsLow‐power saturation was achieved by a train of 80 pulses of duration tp = 20 ms (total saturation time tsat= 3.2 seconds at 50% duty cycle) with B1= 0.6 μT at 54 irradiation frequency offsets. With the 3D snapshot CEST sequence, a 180 × 220 × 54 mm field of view was acquired in 7 seconds per offset. Spectrally selective CEST effects at +3.5 and –3.5 ppm were quantified using multi‐Lorentzian fitting. Reproducibility was high with an intersubject coefficient of variation below 10% in CEST contrasts. Amide and nuclear overhauser effect CEST effects showed similar correlations in tumor and necrosis as show in previous ultra‐high field work.ConclusionA sophisticated CEST tool ready for clinical application was developed and tested for feasibility.