bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative transcriptome analysis of sinonasal inverted papilloma and associated squamous cell carcinoma: Out‐HOXing developmental genes
Authors: Bell Diana , Bell Achim H. , Kupferman Michael E. , Prieto Victor G. , Weber Randal S. , Hanna Ehab Y. 
Published: May 2019

AbstractBackgroundSinonasal papilloma has a tendency toward local destruction, recurrence, and malignant transformation. This study aimed to unravel mechanisms in the malignant transformation of sinonasal papillomas using RNA‐seq.MethodsThe cohort consisted of 37 consecutive patients; tumor histology included a continuum spectrum (sinonasal papillomas/dysplastic/carcinomas‐in‐situ/invasive squamous cell carcinomas). These were microdissected and RNA was subjected to whole‐transcriptome shotgun sequencing.ResultsRNA‐seq and pathway analysis showed that the highest expressed genes/potential drivers were development‐ and differentiation‐related genes. The protein expression of six highly upregulated genes (HOXA9, EN1, DUX4, CA9, CD1a, and CK5/6) validated the RNA‐seq results. HOXA9 and CA9 were found to be expressed in most of the carcinoma samples but were largely negative in papillomas; all of the CA9‐negative carcinomas were recurrent.ConclusionsWe conclude that sinonasal carcinomas arising from papillomas are mainly defined by overexpressed developmental/homeobox genes, which provide the potential for transformation/plasticity, along with differentiation and proliferation behavior of neoplastic cells. Our results support HOXA9 and CA9 as biomarkers for carcinomas, with CA9 emerging as a predictive marker of recurrence.
Synergetic effect of chemical and topological signals of gingival regeneration scaffold on the behavior of human gingival fibroblasts
Authors: Yang Moyang , Guo Zhenzhao , Li Tong , Li Jing , Chen Liyu , Wang Junmei , Wu Jincheng , Wu Zhe 
Published: May 2019

AbstractAchievement of gingiva regeneration poses a substantial challenge for dental aesthetics and periodontal disease repair, but reports of a bioactive and easily available gingival regeneration scaffold are rare. Cell behaviors can be affected by multiple kinds of bioactive signals; thus, it is important to explore the effects of the chemical and topological signals of the scaffold on the behavior of human gingival fibroblasts (HGFs). We hypothesized that the synergetic effect of the chemical and topological scaffold signals were beneficial to gingival regeneration. In this study, HGF behavior on random/aligned poly (e‐caprolactone) (PCL) and polydopamine (PDA)‐coated PCL electrospun scaffolds was investigated in a common medium and in basic fibroblast growth factor (bFGF) medium. The results showed that the synergistic effect of three signals was better than that of one or two signals. The cell proliferation of the aligned scaffold group was higher than that of the random scaffold, and the PCL/PDA‐Aligned+bFGF group showed the highest cell proliferation. Even if two chemical signals were present, the HGFs still maintained an ordered distribution on the aligned scaffold. Cell differentiation and protein secretion analysis indicated that gene and protein expression of focal adhesion kinase and fibronectin were the highest in the PCL/PDA‐Aligned+bFGF group. Taken together, the chemical and topographic signals within the electrospun scaffold were considered to display a synergistic effect on HGF behaviors, suggesting the potential usefulness of the PCL/PDA‐Aligned+bFGF scaffold for gingiva tissue engineering.
Composition control in biphasic silicate microspheres on stimulating new bone regeneration and repair of osteoporotic femoral bone defect
Authors: Ghamor‐Amegavi Edem P. , Yang Xianyan , Qiu Jiandi , Xie Lijun , Pan Zhijun , Wang Juncheng , Zhang Xiangfeng , Ke Xiurong , Zhao Tengfei , Zhang Lei , Gou Zhongru 
Published: May 2019

AbstractApplication of bioactive materials as synthetic bone graft substitutes in regenerative medicine has seen great evolution over the past decades in treating challengeable bone defects. However, balancing the preparation conditions and biological performances of inorganic biomaterials remain a great challenge, especially when there is lack of biomaterial design on how to control component distribution and how pathological bone responds to the biomaterial stimulations and osteogenesis. Here, our objective is to develop yolk‐shell Ca‐silicate microspheres and to investigate the potential biological performances to overcome the limitations in repair of osteoporotic bone defects. The introduction of β‐calcium silicate (CaSiO3) or mesoporous bioactive glass (MBG) into self‐curing β‐dicalcium silicate (Ca2SiO4) cement shell to form spherical granules (CaSiO3@Ca2SiO4, MBG@Ca2SiO4) was to retain the physicochemical property and/or microstructure of each component for optimizing bioactive ion release that could maximize osteostimulation in osteoporosis. We report a scalable shape‐controlled mild fabrication protocol to yield the yolk‐shell granules, endowing to different phases in yolk layer and interconnected macropore networks in the closely packed granule scaffolds. This unique heterostructure preparation is governed by coaxially aligned bilayer nozzle, inorganic powders and biocompatible binders. Extensive in vitro and in vivo evaluation showed that the CaSiO3@Ca2SiO4 and MBG@Ca2SiO4 granules exhibited many superior properties such as controllable ion release, improved biodegradation and enhanced osteogenic capability in comparison with the pure Ca2SiO4@Ca2SiO4, thereby opening new mild‐condition approach in fabricating osteogenesis‐tailored silicate biomaterials for bone regenerative medicine, especially for efficient reconstruction of challenging pathological bone defects.
Identification of pathological grade and prognosis‐associated lncRNA for ovarian cancer
Authors: Chen Ying , Bi Fangfang , An Yuanyuan , Yang Qing 
Published: May 2019

AbstractOvarian carcinoma (OC) is one of the most common malignant tumors in female genitals. In recent years, the therapeutic effect of OC has been significantly improved through the application of effective chemotherapy regimen. However, the 5‐year survival rate is also lower than 30% due to high rate of relapse. So, it is needed to screen reliable predictive and prognostic markers of OC. Ovarian cancer gene expression data and corresponding clinical data used were downloaded from Gene Expression Omnibus database. Weighted gene expression network analysis (WGCNA) and Cox proportional hazards regression (PHR) were used to screen Pathological Grade and Prognosis‐associated long noncoding RNA (lncRNA). Kaplan‐Meier analysis and receiver operating characteristic curves analysis were performed to evaluate the predictive ability of the selected lncRNA. Gene Ontology (GO) enrichment and Gene Set Enrichment Analysis (GSEA) enrichment analysis methods were used to explore the possible mechanisms of the selected lncRNA affecting the development of OC. Five reliably lncRNAs (LINC00664, LINC00667, LINC01139, LINC01419, and LOC286437) was identified through a series of bioinformatics methods. In testing cohorts, we found that the five lncRNAs in predicting the risk of OC recurrence is robustness, and multivariate Cox PHR analysis indicate that the five lncRNAs is an independent risk factor for OC recurrence. Moreover, GO and GSEA enrichment analysis showed that the five lncRNAs are involved in multiple ovarian cancer occurrence mechanism. In summary, all these findings indicated that the five lncRNAs can effectively predict the risk of recurrence of ovarian cancer.
Interaction between 12p chromosomal abnormalities and Lnc‐HOTAIR mediated pathway in acute myeloid leukemia
Authors: El‐Khazragy Nashwa , Ghozy Sherief , Matbouly Safa , Zaki Walid , Safwat Gehan , Hussien Ghada , Khalifa Omar 
Published: May 2019

AbstractObjectivesTo investigate the correlation of homeobox (HOX) transcript antisense RNA expression with clinicopathological features and the clinical prognosis of the patients with chromosome 12p abnormalities associated acute myeloid leukemia (AML). We also investigate the association of 12p chromosomal on the expression of HOTAIR, miRNA‐193a, and c‐kit gene as targeting genes for HOTAIR in AML.MethodsAML patients with 12p chromosomal abnormalities were recruited and compared to AML with other chromosomal abnormalities rather than 12p. The long noncoding RNA (lncRNA) “HOTAIR,” miR‐193a, and c‐Kit genes expression were measured in bone marrow samples using Syber green based real‐time polymerase chain reaction.ResultsWe found a significant difference for the expression levels of HOTAIR, c‐kit, and miR‐193a between 12p abnormalities associated AML and those without. The survival analysis revealed that patient's with low expression levels of HOTAIR and c‐kit had significantly better survival and leukemia free survival. In contrast, miR‐193a was associated with better overall survival but not leukemia free survival.Conclusion12p abnormalities associated AML were associated with worse prognosis. Our results proved that HOTAIR, miR‐193a, and c‐kit genes are independent prognostic predictors in 12p chromosomal associated AML; therefore it may represent a novel therapeutic application in AML in the future.
Overexpression of circ_0005198 sponges miR‐1294 to regulate cell proliferation, apoptosis, migration, and invasion in glioma
Authors: Wang Jiandong , Li Jie , Wang Hongwei , Lv Luting , Sun Jinghui 
Published: May 2019

AbstractGlioma (GM) is an aggressive malignancy with high mortality rate across the world. Mounting studies demonstrates that abnormally expressed circular RNAs (circRNAs) act as important roles in tumor progression. In the current work, a novel circRNA, circ_0005198, was explored. Circ_0005198 level in GM tissue samples and cells was detected. The clinical implication of circ_0005198 was analyzed by Fisher's test and Kaplan‐Meier analysis. In vitro experiments were carried out to elucidate the influence of circ_0005198 on GM cell proliferation, apoptosis, and metastatic properties. Luciferase reporter and rescue experiments were conducted to clear the mechanism of circ_0005198. The expression of circ_0005198 was enhanced in GM tumors and cells. Its expression in tumor specimens was related to clinical severity and poor prognosis. Functionally, circ_0005198 could remarkably boost cell growth, clone‐forming ability, and metastatic properties and attenuate cell apoptosis in GM cells. What's more, circ_0005198 could directly sponge miR‐1294 to exert oncogenic functions. In summary, this study might provide an effective therapeutic target for GM.
Biodegradable collagen from Scomberomorus lineolatus skin for wound healing dressings and its application on antibiofilm properties
Authors: Abinaya M. , Gayathri M. 
Published: May 2019

AbstractThe major resolution of the study was to develop a dynamic form of natural biopolymer material to improve the wound healing by inhibition of biofilm formation on the surface. The extraction of collagen was effectively prepared from Scomberomorus lineolatus fish skin. Lyophilized collagen sheet was liquefied in 0.5M acetic acid to form acidic solubilized collagen (ASC) for further analysis. Physicochemical characterization of ASC was performed by various techniques using a standard protocol. The yield of ASC form S.lineolatus is higher (21.5%) than the previous reported studies. The effect of collagen solubility is gradually decreases with increasing concentration of NaCl and collagen is mostly soluble in acidic pH conditions. The sodium dodecyl sulfate‐polyacrylamide gel electrophoresis analysis of ASC contains α chain composition of α1 and α2 subunits and was characterized as type I collagen. Ultraviolet absorption was regulated as the appropriate wavelength to optimize the collagen. Fourier‐transform infrared spectroscopy and X‐ray diffraction confirmed that the isolated collagen is a triple‐helical structure. The biofilm formation of Pseudomonas aeruginosa was significantly reduced by collagen incorporated with isolated 3,5,7‐trihydroxyflavone (collagen‐TF) sheet up to 70%. The 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl‐tetrazolium bromide assay executed on fibroblast cell lines (L929) shows that the collagen‐TF sheet was 100% compatible to enrich the cell adhesion and proliferation. The current study was the first report to extract, purify, and characterize ASC from S. lineolatus fish skin and characterize as type I collagen. Based on the result, we design the natural biodegradable collagen loaded with TF compound (collagen‐TF) for antibiofilm properties. Compared with different sources of polymer, fish skin collagen is more effective and can be used as a biopolymer sheet for wound healing, food, drug delivery, tissue engineering, and pharmaceutical application.
NEAT1 promotes retinoblastoma progression via modulating miR‐124
Authors: Wang Lufei , Yang Defeng , Tian Rui , Zhang Hui 
Published: May 2019

AbstractThe long noncoding RNA nuclear‐enriched abundant transcript 1 (NEAT1) is reportedly involved in the initiation and progression of cancers of several types. However, the role, expression status, and the detailed mechanism of NEAT1 in retinoblastoma (RB) yet need to be unraveled. We explored the role and the mechanism of NEAT1 activity in RB. Our data show enhanced NEAT1 expression in RB‐affected tissues compared with the corresponding control. Functional experiments reveal that a NEAT1 knockdown in RB cells significantly inhibits proliferation, cycle progression, and facilitates apoptosis and caspase‐3 and ‐9 activities. Besides that, miR‐124 was predicted to be a target of NEAT1 and its reduced expression, as well as the inverse correlation of NEAT1 with miR‐124, was observed in RB‐affected tissues. Further, luciferase and RNA immunoprecipitation (RIP) assays confirmed the interaction between NEAT1 and miR‐124. Rescue experiments confirmed that the inhibition of miR‐124 could reverse the effect of NEAT1 on RB cell proliferation, cycle arrest, apoptosis, and caspase‐3 and ‐9 activities. Thus, NEAT1 promotes RB progression by sponging miR‐124, providing a therapeutic target for RB.
The maximum, supremum, and spectrum for critical set sizes in (0,1)‐matrices
Authors: Cavenagh Nicholas J. , Wright Liam K. 
Published: May 2019

AbstractIf is a partially filled‐in (0,1)‐matrix with a unique completion to a (0,1)‐matrix (with prescribed row and column sums), then we say that is a defining set for . A critical set is a minimal defining set (the deletion of any entry results in more than one completion). We give a new equivalent definition of critical sets in (0,1)‐matrices and apply this theory to , the set of (0,1)‐matrices of dimensions with uniform row and column sum . The smallest possible size for a defining set of a matrix in is [N. Cavenagh, J. Combin. Des. 21 (2013), pp. 253–266], and the infimum (the largest‐smallest defining set size for members of ) is known asymptotically [N. Cavenagh and R. Ramadurai, Linear Algebra Appl. 537 (2018), pp. 38–47]. We show that no critical set of size larger than exists in an element of and that there exists a critical set of size in an element of for each such that . We also bound the supremum (the smallest‐largest critical set size for members of ) between and .
Multiple senses of community and recovery processes. A pilot study for a national evaluation of the experiences of persons with substance use problems receiving help and services from Norwegian municipalities
Authors: Bahl Nina Kavita Heggen , Nafstad Hilde Eileen , Blakar Rolv Mikkel , Landheim Anne Signe , Brodahl Morten 
Published: May 2019

AbstractAimsThis pilot study uses a multifaceted concept of sense of community (SOC)—multiple senses of community (MPSOC)—to understand how the multiple communities of persons with substance use problems, including those with a positive, negative and neutral SOC, influence processes of substance use recovery.MethodsSemi‐structured interviews were conducted with 16 informants from different Norwegian municipalities and regions. A collaborative research design and thematic analyses with a peer researcher were applied.ResultsThe findings confirm prior findings of key ingredients related to recovery. However, they also illustrate that for communities to promote recovery, they need to fulfil individual needs, provide distance from pretreatment status, identity and roles and harmonise with individual meaning systems of an ideal community.ConclusionExperiences of positive and negative community connections within geographical, relational and ideal communities take part in recovery processes. Community participation is suggested to be included in individual outpatient treatment and posttreatment plans.
Transcription factor 7‐like 2‐associated signaling mechanism in regulating cementum generation by the NF‐κB pathway
Authors: Zhao Zifan , Dai Jing , Yin Chengcheng , Wang Xuzhu , Wang Jinyang , Jia Xiaoshi , Zhao Qin , Fu Hui , Zhang Yufeng , Xia Haibin 
Published: May 2019

AbstractCementum regeneration is an important and challenging stage in periodontal tissue engineering and regeneration. Pathosis of the periodontium, including cementum, is important in precision diagnosis and obstinate treatment of systemic diseases, such as diabetes, leukemia, and Acquired Immune Deficiency Syndrome. Here, we found that during periodontium development, transcription factor 7‐like 2 (Tcf7l2) was widely expressed in the periodontium and dental sac. In mouse cementoblast cell line (OCCM‐30), the activation of NF‐κB and cementoblast mineralization was significantly reduced when Tcf7l2 gene was silenced. Moreover, Tcf7l2 has a positive effect on NF‐κB and cementoblast mineralization. Therefore, Tcf7l2 promotes cementum formation through the NF‐κB pathway. In addition, we found a decreased expression of phosphorylated p65 and a thin layer of cementum in Tcf7l2fl/fl mice. These results suggest that Tcf7l2, which accelerates cementum formation by activating NF‐κB, has great potential in the treatment of periodontitis and provide guidance for periodontal tissue regeneration.
Modulation of Myb‐induced NF‐kB ‐STAT3 signaling and resulting cisplatin resistance in ovarian cancer by dietary factors
Authors: Tian Miao , Tian Dan , Qiao Xiaofang , Li Jinlong , Zhang Leilei 
Published: May 2019

Abstractc‐Myb regulates tumorigenesis in multiple cancers. Here we show, for the first time, the mechanism of c‐Myb‐mediated proliferation, invasion, and drug resistance in ovarian cancer (OC), the most lethal gynecological cancer, and a comparative analyses of dietary agents, curcumin, epigallocatechin‐3‐gallate (EGCG), and sulforaphane in inhibiting c‐Myb activity. We evaluated myb expression in patients with OC and found its increased expression in patients with cancer, compared with normal controls and in higher grade tumors, compared with low‐grade tumors. Using ES2 and OVCAR3 cell line models, along with the silencing or overexpression of c‐Myb, we establish a role of c‐Myb in determining resistance to cisplatin. c‐Myb overexpression activated NF‐κB and STAT3 signaling leading to enhanced proliferation, invasion, and cisplatin resistance. Contrary to this, silencing of c‐Myb inhibited proliferation, invasion, and sensitized OC cells to cisplatin. Further, among the dietary agents tested, EGCG almost completely inhibited the c‐Myb‐induced proliferation and invasion whereas sulforaphane also had significant inhibitory effect. Both compounds significantly sensitized OC cells to cisplatin, reversing the c‐Myb effects. Higher c‐Myb levels in patients with ovarian cancer lead to poor survival and our results indicate a possible effect of dietary factors EGCG and sulforaphane against c‐Myb‐mediated ovarian cancer progression and chemoresistance.
Impact of preoperative body compositions on survival following resection of biliary tract cancer
Authors: Yoon Seung Bae , Choi Moon Hyung , Song Meiying , Lee Ju Hyun , Lee In Seok , Lee Myung Ah. , Hong Tae Ho , Jung Eun Sun , Choi Myung‐Gyu 
Published: May 2019

AbstractBackgroundAlthough surgical resection is the only potentially curative treatment for biliary tract cancer, the prognosis remains poor after a major operation such as pancreatoduodenectomy or hepatectomy. We aimed to investigate the impact of preoperative body compositions on long‐term survival of patients undergoing resection of biliary tract cancer.MethodsWe analysed data of patients diagnosed with biliary tract cancer who underwent surgery from 2009 to 2015. Skeletal muscle area, skeletal muscle radiation attenuation, and visceral and subcutaneous adipose tissue areas were measured from the computed tomography images at L3 vertebral levels obtained before resection of cancer. Patients were divided into two groups based on the sex‐specific median values for each parameter, and long‐term survival was compared between the groups.ResultsA total of 371 patients (women, 39.6%; mean age, 66.2 ± 9.6 years) were finally included in the analysis. Patients with low skeletal muscle index (SMI) had significantly shorter median survival than those with high SMI (29 vs. 39 months; P = 0.026). Patients with low skeletal muscle attenuation (SMA) also showed reduced survival compared with those with high SMA (median survival 25 vs. 60 months; P = 0.002). Combining these two factors, survival was highest in the high SMI/high SMA group (reference) and lowest in the low SMI/low SMA group (hazard ratio, 2.18; 95% confidence interval, 1.44–3.30). Visceral and subcutaneous adipose tissue areas were not associated with long‐term survival.ConclusionsLow SMI and low SMA on computed tomography scan have a negative impact on survival after resection of biliary tract cancer. They can be used in preoperative risk assessment to assist in treatment decision making.
Inappropriate use of the emergency department for nonurgent conditions: Patient characteristics and associated factors at a Japanese hospital
Authors: Miyazawa Asako , Maeno Takami , Shaku Fumio , Tsutsumi Madoka , Kurihara Hiroshi , Takayashiki Ayumi , Kohno Mototsugu , Suzuki Masatsune , Maeno Tetsuhiro 
Published: May 2019

AbstractBackgroundThe purpose of this study was to investigate the primary factors associated with inappropriate out‐of‐hours emergency department (ED) use by patients with nonurgent conditions.MethodsWe compared patients with nonurgent conditions who made inappropriate, out‐of‐hours ED visits to patients who visited an acute care hospital during daytime consultation hours between May 30 and October 16, 2014, in terms of patient characteristics and reasons for consultation. Our goal was to identify factors associated with inappropriate ED use, defined as an out‐of‐hours, nonurgent, and unnecessary visits.ResultsWe analyzed data from 84 patients who made inappropriate use of out‐of‐hours emergency care and 147 patients who sought care during regular consultation hours. In the inappropriate use group, “desire to be cured quickly” was the most common reason. Acute upper respiratory infection, acute gastroenteritis, and primary headache comprised 51.1% of diagnoses in the inappropriate use group. One factor associated with inappropriate use was two or more previous out‐of‐hours ED visits (odds ratio (OR) 3.19; 95% confidence interval (CI) 1.22‐8.31) (reference: 0 visits).ConclusionsPatients with two or more previous out‐of‐hours ED visits were more than three times as likely to inappropriately use the ED compared to patients who had not visited the ED at all in the past 3 years.
How many randomly colored edges make a randomly colored dense graph rainbow Hamiltonian or rainbow connected?
Authors: Anastos Michael , Frieze Alan 
Published: May 2019

AbstractIn this paper, we study the randomly edge colored graph that is obtained by adding randomly colored random edges to an arbitrary randomly edge colored dense graph. In particular, we ask how many colors and how many random edges are needed so that the resultant graph contains a fixed number of edge‐disjoint rainbow‐Hamilton cycles w.h.p. We also ask when, in the resultant graph, every pair of vertices is connected by a rainbow path w.h.p.