bluuebunny/crossref_metadata_2025_split huggingface dataset:
HDAC3 inhibition prevents oxygen glucose deprivation/reoxygenation‐induced transendothelial permeability by elevating PPARγ activity in vitro
Authors: Zhao Qiuchen , Yu Zhanyang , Zhang Fang , Huang Lena , Xing Changhong , Liu Ning , Xu Yun , Wang Xiaoying 
Published: October 2018

AbstractHistone deacetylase 3 (HDAC3), a member of class I HDAC, regulates a wide variety of normal and abnormal physiological functions. Recent experimental studies suggested that inhibition of HDAC3 may increase acetylation of certain key signaling regulating proteins such as peroxisome proliferator‐activated receptor γ (PPARγ), which plays a crucial role in modulating cerebrovascular function and integrity. However, the role of HDAC3 inhibition in cerebrovascular endothelium function under pathological condition has not been fully investigated. In this study, we tested the hypothesis that inhibition of HDAC3 by RGFP966, a highly selective HDAC3 inhibitor, promotes PPARγ activation by enhancing its protein acetylation, resulting in protection of oxygen glucose deprivation and reoxygenation (OGD/R)‐induced increase of transendothelial cell permeability. In cultured primary human brain microvascular endothelial cells, our experimental results show that OGD/R increases transendothelial cell permeability and down‐regulates junction protein expression. While we also detected HDAC3 activity increase and PPARγ activity decline after OGD/R. However, treatment with RGFP966 significantly attenuated the OGD/R‐induced increase of transendothelial cell permeability and down‐regulation of tight junction protein Claudin‐5. These effects were observed to be dependent on HDAC3 activity inhibition‐mediated PPARγ protein acetylation/activation. Lastly, HDAC3 small interfering RNA mimics the protective effects of RGFP966 on human brain microvascular endothelial cells. Taken together, our data indicate that HDAC3 inhibition might comprise a new therapeutic target for reducing blood–brain barrier integrity disruption and vascular dysfunctions in neurological disorders.
image
CAD/CAM Constructed Poly(etheretherketone) (PEEK) Framework of Kennedy Class I Removable Partial Denture: A Clinical Report
Authors: Harb Islam E. , Abdel‐Khalek Elsayed A. , Hegazy Salah A. 
Published: October 2018

AbstractThe prevalence of partially edentulous adults that could be treated by removable partial dentures (RPDs) is estimated to be greater than 20% in some regions of the United States. Problems related to traditional materials for RPD construction have been documented. Advances in polymer‐based materials and digital fabrication strategies would allow increased biocompatibility, durability, and elasticity, as well as more esthetically pleasing and cost‐effective benefits. This article reports on poly(etheretherketone) RPD framework produced by digital and milling technology.
Information received and information needed on electronic prescriptions - Finnish pharmacy customers' experiences during the nationwide implementation
Authors: Lämsä Elina , Timonen Johanna , Ahonen Riitta 
Published: October 2018

Abstract

Objectives
To investigate (1) what Finnish pharmacy customers have learned about the implemented electronic prescriptions (e-prescriptions), (2) from whom or where have they learned about them, (3) whether they are satisfied with the information received and if not, (4) what more information they would like.

Methods
We surveyed 1288 (44%) pharmacy customers aged ≥18 years collecting medicines for themselves with e-prescriptions in 18 pharmacies across Finland in 2015. Descriptive analysis, chi-square and Fisher's exact tests were used in the analysis.

Key findings
Nearly all respondents had received information about e-prescriptions (97%). A physician (67%) and a pharmacy (53%) were the most common information sources. The vast majority of the respondents had learned about how to purchase medicines with an e-prescription (86%). Most of them had also received information about the benefits of e-prescriptions (59%) and how they can view their e-prescriptions on a computer (58%). The majority of pharmacy customers felt they had received sufficient information on e-prescriptions (83%). Those dissatisfied with the information received asked for more information about how e-prescriptions are protected against misuse (47%) and who can view their e-prescriptions (44%).

Conclusions
Most Finnish pharmacy customers have learned how to use e-prescriptions, what their benefits are and how to view e-prescriptions on a computer. The information is generally obtained from physicians and pharmacies. Information needs concern data protection and data security. Customers are mainly satisfied with the information received. However, their knowledge only partly meets the national requirements on the information they should be provided with.
Periodontitis, periodontal inflammation, and mild cognitive impairment: A 5‐year cohort study
Authors: Iwasaki Masanori , Kimura Yumi , Ogawa Hiroshi , Yamaga Takayuki , Ansai Toshihiro , Wada Taizo , Sakamoto Ryota , Ishimoto Yasuko , Fujisawa Michiko , Okumiya Kiyohito , Miyazaki Hideo , Matsubayashi Kozo 
Published: October 2018

Background and ObjectivesIdentification of modifiable factors for mild cognitive impairment (MCI) is important since individuals with MCI are at a high risk of dementia and disability. Previous studies have suggested a potential association between periodontitis and cognitive impairment, but the results remain inconclusive. We designed a 5‐year longitudinal study to explore the association between MCI and periodontitis and periodontal inflammation in older adults.MethodsThis study included 179 community‐dwelling dentate individuals (62 men and 117 women, average age: 80.1 years). A full‐mouth periodontal examination at six sites per tooth was performed at baseline. Case definitions provided by the European Workshop in Periodontology Group C (EWP definition) and the Centers for Disease Control/American Academy of Periodontology (CDC/AAP definition) were used to define severe periodontitis. Additionally, the periodontal inflamed surface area (PISA), reflecting the amount of inflamed periodontal tissue, was calculated using clinical periodontal parameters. Follow‐up cognitive examinations for MCI diagnosis were performed by neurologists 1, 2, 3, and 5 years after baseline. Odds ratios (ORs) for MCI according to the presence of periodontitis and periodontal inflammation at baseline were calculated using multilevel mixed‐effects logistic regression.ResultsAt baseline, 56.4% and 27.4% of the participants had severe periodontitis by the EWP and CDC/AAP definitions, respectively. After adjusting for follow‐up period and other baseline health characteristics (age, sex, smoking status, educational level, physical activity level, obesity, depression, and diabetes), severe periodontitis by either definition was significantly associated with MCI (for the EWP definition: adjusted OR = 3.58, 95% confidence interval [CI] = 1.45‐8.87; for the CDC/AAP definition: adjusted OR = 2.61, 95% CI = 1.08‐6.28). Periodontal inflammation assessed by PISA was also significantly associated with a higher OR for MCI (adjusted OR = 1.05, 95% CI = 1.01‐1.10, per 10‐mm2 increase in PISA).ConclusionSevere periodontitis and periodontal inflammation were associated with incident MCI among older community‐dwelling men and women.
Fiber concentrates from raspberry and blueberry pomace in gluten‐free cookie formulation: Effect on dough rheology and cookie baking properties
Authors: Šarić Bojana , Dapčević‐Hadnađev Tamara , Hadnađev Miroslav , Sakač Marijana , Mandić Anamarija , Mišan Aleksandra , Škrobot Dubravka 
Published: October 2018

AbstractRaspberry and blueberry pomace, by‐products from fruit juice industry, were dehydrated to obtain fruit fiber concentrates and valorized as ingredients in gluten‐free (GF) cookies formulation. The aim was to evaluate the effect of substituting 30% (w/w) of GF flour mixture with different ratios of blueberry/raspberry fiber concentrates (0/30, 15/15, and 30/0) on rheological properties of cookie dough and cookie baking quality. Generally, the incorporation of the fiber concentrates increased the elastic and the viscous moduli thus producing harder doughs and consequently firmer cookies in comparison to the control. Although having similar total, soluble and insoluble fiber content, blueberry fiber concentrates were characterized with higher water absorption capacity and consequently yielded cookies with more rigid dough structure, higher water loss during baking, lower cookie thickness, higher spread ratio, more dense inner structure, and increased cookie hardness than those containing raspberry fiber concentrates. Enrichment of GF cookies with raspberry and blueberry fiber concentrates resulted in products with dietary fiber content higher than 6 g per 100 g, thus making them candidates for bearing “high fiber” nutrition claim.Practical applicationsCurrently available GF bakery products mostly lack dietary fibers (DFs) and other important nutrients, because they are usually manufactured from refined flour and/or starches. As the products with long shelf‐life, very popular in daily diet of almost all consumer profiles, cookies could represent good matrices for the addition of functional ingredients, such as DFs. In this paper, GF cookies were enriched with the fiber concentrates recovered from blueberry and raspberry pomace. Application of these fiber sources enabled recycling of fruit processing industry by‐products into novel, “high fiber” GF product with improved nutritive profile.
Saliva could act as an emulsifier during oral processing of oil/fat
Authors: Glumac Miodrag , Qin Lanxi , Chen Jianshe , Ritzoulis Christos 
Published: October 2018

AbstractHuman saliva is a fluid naturally secreted in the oral cavity that interacts with food and food components for bolus formation, structure degradation, as well as lubrication. Because of the presence of salivary proteins, we speculate that saliva could also function as an effective emulsifier during oral processing of oil/fat. In this preliminary work, experiments were then designed to test this hypothesis. Whole human saliva from three healthy subjects were collected and analyzed for protein content, surface tension, and molecular weight distribution. Saliva emulsions were obtained both in vitro one and in situ for all three participating subjects. Droplet size distribution, zeta potential, and microstructure of such emulsions were examined immediately after the emulsification. Results show that stable saliva emulsions can be produced during oral processing of either pure oil (rapeseed oil) or fat food (pork belly in this work). Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS‐PAGE) analysis showed that protein fractions of 27 and 55 kDa molecular weights were favored for emulsion formation. This work suggests that human saliva could function as an effective emulsifier and oral emulsification could be an important mechanism for the oral processing of oil/fat. Despite being preliminary, findings from this work provide a new scientific insight to our understanding of the oral behavior of oil/fat and their sensory perception.Practical applicationsFood industry is currently under a growing pressure to use novel techniques and ingredients to minimize the use of oil/fat in food products but without compromising its sensory quality. However, food industry has limited progresses because of the lack of understanding of the mechanisms of oral sensation and perception of oil/fat. Whereas there have been extensive debates about the sensory mechanisms of oil/fat, this work takes a step back by examining the oral behavior of oil/fat. Findings show that saliva can actually function as emulsifier to oil/fat, which means that ingested oil/fat will be dispersed and converted into an emulsion at the oral stage. The findings from this work offer food industry new insight on the sensory mechanisms of oil/fat.
Shock stage distribution of 2280 ordinary chondrites—Can bulk chondrites with a shock stage of S6 exist as individual rocks?
Authors: Bischoff Addi , Schleiting Maximilian , Patzek Markus 
Published: October 2018

AbstractThe brecciation and shock classification of 2280 ordinary chondrites of the meteorite thin section collection at the Institut für Planetologie (Münster) has been determined. The shock degree of S3 is the most abundant shock stage for the H and LL chondrites (44% and 41%, respectively), while the L chondrites are on average more heavily shocked having more than 40% of rocks of shock stage S4. Among the H and LL chondrites, 40–50% are “unshocked” or “very weakly shocked.” Considering the petrologic types, in general, the shock degree is increasing with petrologic type. This is the case for all meteorite groups. The main criteria to define a rock as an S6 chondrite are the solid‐state recrystallization and staining of olivine and the melting of plagioclase often accompanied by the formation of high‐pressure phases like ringwoodite. These characteristics are typically restricted to local regions of a bulk chondrite in or near melt zones. In the past, the identification of high‐pressure minerals (e.g., ringwoodite) was often taken as an automatic and practical criterion for a S6 classification during chondrite bulk rock studies. The shock stage classification of many significantly shocked chondrites (>S3) revealed that most ringwoodite‐bearing rocks still contain more than 25% plagioclase (74%). Thus, these bulk chondrites do not even fulfill the S5 criterion (e.g., 75% of plagioclase has to be transformed into maskelynite) and have to be classified as S4. Studying chondrites on typically large thin sections (several cm2) and/or using samples from different areas of the meteorites, bulk chondrites of shock stage S6 should be extremely rare. In this respect, the paper will discuss the probability of the existence of bulk rocks of S6.
Spatiotemporal variations in microbial diversity across the three domains of life in a tropical thalassohaline lake (Dziani Dzaha, Mayotte Island)
Authors: Hugoni Mylène , Escalas Arthur , Bernard Cécile , Nicolas Sébastien , Jézéquel Didier , Vazzoler Fanny , Sarazin Gerard , Leboulanger Christophe , Bouvy Marc , Got Patrice , Ader Magali , Troussellier Marc , Agogué Hélène 
Published: October 2018

AbstractThalassohaline ecosystems are hypersaline environments originating from seawater in which sodium chloride is the most abundant salt and the pH is alkaline. Studies focusing on microbial diversity in thalassohaline lakes are still scarce compared with those on athalassohaline lakes such as soda lakes that have no marine origin. In this work, we investigated multiple facets of bacterial, archaeal and eukaryotic diversity in the thalassohaline Lake Dziani Dzaha using a metabarcoding approach. We showed that bacterial and archaeal diversity were mainly affected by contrasting physicochemical conditions retrieved at different depths. While photosynthetic microorganisms were dominant in surface layers, chemotrophic phyla (Firmicutes or Bacteroidetes) and archaeal methanogens dominated deeper layers. In contrast, eukaryotic diversity was constant regardless of depth and was affected by seasonality. A detailed focus on eukaryotic communities showed that this constant diversity profile was the consequence of the high predominance of Picocystis salinarum, while nondominant eukaryotic groups displayed seasonal diversity turnover. Altogether, our results provided an extensive description of the diversity of the three domains of life in an unexplored extreme environment and showed clear differences in the responses of prokaryotic and eukaryotic communities to environmental conditions.
Extreme copy number variation at a tRNA ligase gene affecting phenology and fitness in yellow monkeyflowers
Authors: Nelson Thomas C. , Monnahan Patrick J. , McIntosh Mariah K. , Anderson Kayli , MacArthur‐Waltz Evan , Finseth Findley R. , Kelly John K. , Fishman Lila 
Published: October 2018

AbstractCopy number variation (CNV) is a major part of the genetic diversity segregating within populations, but remains poorly understood relative to single nucleotide variation. Here, we report on a tRNA ligase gene (Migut.N02091; RLG1a) exhibiting unprecedented, and fitness‐relevant, CNV within an annual population of the yellow monkeyflower Mimulus guttatus. RLG1a variation was associated with multiple traits in pooled population sequencing (PoolSeq) scans of phenotypic and phenological cohorts. Resequencing of inbred lines revealed intermediate‐frequency three‐copy variants of RLG1a (trip+; 5/35 = 14%), and trip+ lines exhibited elevated RLG1a expression under multiple conditions. trip+ carriers, in addition to being over‐represented in late‐flowering and large‐flowered PoolSeq populations, flowered later under stressful conditions in a greenhouse experiment (p < 0.05). In wild population samples, we discovered an additional rare RLG1a variant (high+) that carries 250–300 copies of RLG1a totalling ~5.7 Mb (20–40% of a chromosome). In the progeny of a high+ carrier, Mendelian segregation of diagnostic alleles and qPCR‐based copy counts indicate that high+ is a single tandem array unlinked to the single‐copy RLG1a locus. In the wild, high+ carriers had highest fitness in two particularly dry and/or hot years (2015 and 2017; both p < 0.01), while single‐copy individuals were twice as fecund as either CNV type in a lush year (2016: p < 0.005). Our results demonstrate fluctuating selection on CNVs affecting phenological traits in a wild population, suggest that plant tRNA ligases mediate stress‐responsive life‐history traits, and introduce a novel system for investigating the molecular mechanisms of gene amplification.
Altitudinal variation of the gut microbiota in wild house mice
Authors: Suzuki Taichi A. , Martins Felipe M. , Nachman Michael W. 
Published: October 2018

AbstractThe maintenance of oxygen homeostasis in the gut is critical for the maintenance of a healthy gut microbiota. However, few studies have explored how the concentration of atmospheric oxygen affects the gut microbiota in natural populations. High‐altitude environments provide an opportunity to study the potential effects of atmospheric oxygen on the composition and function of the gut microbiota. Here, we characterized the caecal microbial communities of wild house mice (Mus musculus domesticus) in two independent altitudinal transects, one in Ecuador and one in Bolivia, from sea level to nearly 4,000 m. First, we found that differences in altitude were associated with differences in the gut microbial community after controlling for the effects of body mass, diet, reproductive status and population of origin. Second, obligate anaerobes tended to show a positive correlation with altitude, while all other microbes tended to show a negative correlation with altitude. These patterns were seen independently in both transects, consistent with the expected effects of atmospheric oxygen on gut microbes. Prevotella was the most‐enriched genus at high elevations in both transects, consistent with observations in high‐altitude populations of pikas, ruminants and humans, and also consistent with observations of laboratory mice exposed to hypoxic conditions. Lastly, the renin–angiotensin system, a recently proposed microbiota‐mediated pathway of blood pressure regulation, was the top predicted metagenomic pathway enriched in high altitudes in both transects. These results suggest that high‐altitude environments affect the composition and function of the gut microbiota in wild mammals.
The economics of the super‐multiplier: A comprehensive treatment with labor markets
Authors: Palley Thomas 
Published: October 2018

AbstractThis paper links the super‐multiplier to Keynesian macroeconomics, showing it to be the most Keynesian of growth perspectives. Next, the paper shows that the super‐multiplier is a micro‐economically coherent theory of investment and capital accumulation. Firms’ decisions regarding capital accumulation coordinate demand and supply growth in goods markets. The paper then explores the implications of incorporating the super‐multiplier in the neo‐Kaleckian and Cambridge growth models. Lastly, it shows how labor markets and unemployment can be added into super‐multiplier models to provide a comprehensive growth model that addresses Solow's (1956, Journal of Economics, 70, 65–94) labor market knife‐edge problem. Incorporating labor markets does not change the fundamental super‐multiplier result that growth is determined by the growth of autonomous demand.
Multi‐level analysis of national nursing students’ disclosure of patient safety concerns
Authors: Palese Alvisa , Gonella Silvia , Grassetti Luca , Mansutti Irene , Brugnolli Anna , Saiani Luisa , Terzoni Stefano , Zannini Lucia , Destrebecq Anne , Dimonte Valerio ,   the SVIAT TEAM
Published: October 2018

ContextError reporting is considered one of the most important mediating factors for patient safety (PS). However, reporting errors can be challenging for health care students.ObjectivesThe aims of the study were: (i) to describe nursing students’ opportunity to report errors, near misses or PS issues that emerged during their clinical learning experience; and (ii) to explore associated factors of the process of reporting itself.MethodsA national survey was conducted on 9607 (91.7%) undergraduate nursing students. The endpoint was to have reported PS issues in the last clinical learning experience (from 0 ‘never’ to 3 ‘always’). Explanatory variables were set individual, nursing programme and regional levels.ResultsA total of 4004 (41.7%) nursing students reported PS issues from ‘never/rarely’ to ‘sometimes’. In the multi‐level analysis, factors increasing the likelihood of reporting events affecting PS have been mainly at the nursing programme level: specifically, higher learning opportunities (odds ratio [OR] = 3.040; 95% confidence interval [CI], 2.667–3.466), self‐directed learning opportunities (OR = 1.491; 95% CI, 1.364–1.630), safety and nursing care quality (OR = 1.411; 95% CI, 1.250–1.594) and quality of tutorial strategies OR = 1.251; 95% CI, 1.113–1.406). By contrast, being supervised by a nurse teacher (OR = 0.523; 95% CI, 0.359–0.761) prevented the disclosure of PS issues compared with being supervised by a clinical nurse. Students attending their nursing programmes in some Italian regions showed a higher likelihood (OR from 1.346 to 2.938) of reporting PS issues compared with those undertaking their education in other regions.ConclusionsNursing students continue to be reticent to report PS issues. Given that they represent the largest generation of future health care workers, their education regarding PS should be continuously monitored and improved; moreover, strategies aimed at developing a non‐blaming culture should be designed and implemented both at the clinical learning setting and regional levels.