bluuebunny/crossref_metadata_2025_split huggingface dataset:
Non-hormonal methods of correction of climacteric disorders in women
Authors: Tatarchuk T.F. , Reheda S.I. , Kosei N.V. , Zakharenko N.F. , Plaksiieva K.D. 
Published: December 2024

Objective of the study: to optimize non-hormonal therapy of menopausal syndrome in perimenopausal women.
Materials and methods. 60 female patients aged 40–55 years with mild climacteric disorders were examined. Group I included 30 women who received a psychological consultation with recommendations on lifestyle and dietary correction, group II included 30 women who received Ayurvedic herbal drug Zintago 10 ml three times a day for three months a psychological consultation with recommendations on lifestyle and dietary correction. Patients were tested before treatment and 1 and 3 months after therapy according to the following scales: Spielberger-Hanin, PSM-25, MRS, and according to the SF-36 scale before treatment and 3 months after.
Results. 1 (31.4 ± 1.9 points) and 3 (27.8 ± 2.3 points) months after treatment in the II group there was a significant (p < 0.05) decrease in the indicator of personal anxiety according to the Spielberger scale in comparison with the initial level. There was a decrease in the index of situational anxiety from 36.4 ± 1.9 points before treatment to 26.2 ± 1.7 points (p < 0.05) in group II after 3 months of therapy. There were no significant clinical results in group I.
According to the PSM-25 scale, a clinically significant effect was in both groups after 3 months of treatment, but in group II it was achieved faster, after only one month – from 154.7 ± 9.6 to 122.6 ± 8.3 points (p < 0.05).
According to the SF-36 scale, a significant improvement in the quality of life in group I after 3 months of therapy was only in the indicators of social functioning (from 54.6 ± 7.1 points before treatment to 73.8 ± 5.8 after 3 months of therapy, p < 0.05) and role functioning due to emotional state (from 57.3 ± 6.6 to 74.8 ± 4.6 points, p < 0.05), while patients of group II demonstrated a significant improvement in all domains of the SF-36 scale.
The somato-vegetative component of the MRS scale in the II group before treatment was 3.7 ± 0.06, and after 1 and 3 months it decreased to 3.4 ± 0.04 and 2.6 ± 0.06 points, respectively, (р < 0 ,05).
Already after 3 months of treatment there was a significant decrease in the indicators of psycho-emotional changes in all women, which indicates the feasibility of using psychoprophylactic drugs in patients with mild menopausal syndrome.
Conclusions. Psychological counseling can be used in perimenopausal women to correct the manifestations of menopausal syndrome (somato-vegetative and psychological components) and such mental health indicators as social and role functioning. The ayurvedic herbal compositions can enhance the effect of psychological counseling in perimenopausal women with mild menopausal syndrome.
Dynamics of C-reactive protein and albumin levels in women of late reproductive age during pregnancy induced in assisted reproductive technology programs
Authors: Khancha F.A. , Nosenko O.M. 
Published: December 2024

Background. Pregnancy from a biological point of view is an inflammatory condition. Decreased fertility, especially when assisted reproductive technologies are required, as well as women’s late reproductive age is associated with increased inflammation.
Objective of the study: to determine the serum levels of C-reactive protein (CRP) and albumin in women of late reproductive age in the dynamics of pregnancy induced in assisted reproductive technologies programs.
Materials and methods. Under observation were 123 women with infertility cured in assisted reproductive technologies cycles: 65 were pregnant women of late reproductive age (group I), 58 were of active reproductive age (group II). Control group С consisted of 57 pregnant women of advanced reproductive age after the natural concept. The level of CRP was determined using the immunoturbidimetric method, albumin level – with a colorimetric analysis.
Results. It was found that, the CRP level in the blood serum increased over the course of the gestational process and was significantly lower in the first trimester compared to the second and third trimester in all studied groups of pregnant women. Pregnant women of group I had higher levels of serum CRP compared to women of group II and group C. The albumin level in the blood serum in the first trimester of pregnancy was statistically significantly higher compared to the second and third trimesters in all studied groups. Women in group I had lower levels of serum albumin compared to pregnant women in group II and group C.
Conclusions. Pregnancy induced in assisted reproductive technologies programs in women of late reproductive age is characterized by increased levels of CRP and decreased levels of albumin in peripheral blood serum, which requires the development of appropriate therapeutic and preventive correction during pregnancy.
Modern progestogens in the treatment of genital endometriosis
Authors: Markin L.B. , Matvienko O.O. 
Published: December 2024

Objective of the review: to evaluate modern approaches to the use of progestogens for the treatment of endometriosis, using literature sources, to provide examples of their effectiveness in global and own practices.Analysis of literary data. In total, 41 publications by foreign authors were selected and processed. Information on the effectiveness of the domestic progestogen drug Vigest-KV in the treatment of endometriosis in our own practice is also presented.Today, the newest progestin is dienogest, a derivative of 19-nortestosterone. It has shown its high efficiency in various clinical manifestations of endometriosis, as well as in its recurrences. Long-term treatment regimens with dienogest (60 months) are promising, as they effectively reduce pain and the risk of endometriosis recurrence, in particular after surgical treatment. An important advantage of dienogest is the low frequency of side effects (1.2–2.5%), especially such as the effect on bone mineral density.Our clinical experience allows us to recommend the Ukrainian medicinal product Vigest-KV, containing dienogest, for the treatment of various types of endometriosis. Its advantage is a special mechanism of ovulation blockade aimed at apoptosis of the granulosa cells of the growing follicle. Also, long-term use of this drug leads to the inhibition of follicle-stimulating and luteinizing hormones and a moderate decrease in the estradiol, the level of which is within the physiological parameters. This is very important to avoid the symptoms of estrogen deficiency while maintaining the anti-proliferative effect.Conclusions. Today, the main method of treating endometriosis is hormone therapy. It not only reduces the clinical symptoms of endometriosis, but is also used before and after surgical treatment to reduce the risk of disease recurrence. Progestins (dienogest) are the most common hormonal method of treating endometriosis. Dienogest, as the most effective progestin, has a pronounced anti-proliferative effect directed at the endometrium, as well as anti-inflammatory and immunomodulatory effects with a minimal risk of side effects. Dienogest in a dose of 2 mg/day provides a reduction in clinical symptoms, an improvement in the quality of life and preservation of reproductive function. Taking into account our own clinical experience of using Vigest-KV in various types of endometriosis, we can recommend its implementation into clinical practice.
Nos jardins do CAS
Authors: Francisca Cleidimara da Silva  , Pedrosa Giany Paiva , Mendes Veras Mízia Emanuella 
Published: December 2024

A infância é o momento mais marcante quanto ao desenvolvimento motor e cognitivo do ser humano e, por isso, é tão importante tratá-la com respeito e cuidado. Visando uma aprendizagem significativa com base na equidade e foco no conhecimento de mundo, organizou-se um projeto intitulado “Nos Jardins do CAS”, no CAS Mossoró, com nove crianças Surdas na faixa etária entre 4 e 12 anos, que tiveram a oportunidade de conhecer os componentes da fauna e flora locais, suas interações intra e interespecíficas, os impactos dos mesmos no meio ambiente e o papel de cada um dentro dos muros do CAS. A condução do projeto teve início em maio do corrente ano. As crianças foram apresentadas a insetos, aracnídeos, répteis, aves e plantas, observando seu modo de vida, hábitos e peculiaridades. Puderam construir modelos didáticos que as ajudaram na compreensão de conceitos como metamorfose e fotossíntese. Houve o plantio de grãos de feijão, a princípio em copos com algodão e, posterior- mente, em uma mistura de areia e esterco. Tais plantas foram regadas periodicamente para observação do crescimento e desenvolvimento das mesmas, além de um comparativo relativo à quantidade de água a qual elas eram submetidas. Houve a produção de cartazes e outros instrumentos pedagógicos por meio das próprias crianças, que puderam materializar o conhecimento adquirido através de imagens e palavras escritas na Língua Portuguesa (L2). Por fim, foram gravados vídeos onde as crianças explicam o que aprenderam, seja em relação ao conteúdo ou aos sinais de Libras.
Pequenos pesquisadores da educação bilíngue: curiosidades sobre a natureza e a vida dos insetos
Authors: Honorato Elaine Costa , Graciete Figueiredo  
Published: December 2024

Estudar a vida dos insetos é iniciar um processo de conscientização sobre o cuidado com a natureza e o reconhecimento da riqueza deste universo que é minúsculo, mas também carregado de uma grandiosidade fundamental para o meio ambiente. Pensando na relevância deste conhecimento, este estudo apresenta um relato de uma experiência realizada nos 2.º anos do Ensino Fundamental I do Instituto Nacional de Educação de Surdos, localizado na cidade do Rio de Janeiro, e busca trabalhar a interdisciplinaridade por meio das disciplinas de Ciências, Língua Portuguesa Escrita e Libras. A disciplina de Ciências trabalhou o reconhecimento e a importância dos insetos para o meio ambiente, a identificação do seu habitat natural e o respeito à natureza. A disciplina de Língua Portuguesa trabalhou o desenvolvimento da escrita por meio das curiosidades que o estudo desperta a partir da pesquisa realizada nos espaços da escola durante a observação dos insetos encontrados no INES. A disciplina de Libras trabalhou a ampliação do repertório linguístico e a desenvoltura com a Língua Brasileira de Sinais por meio da apresentação de uma maquete construída pelos estudantes após o estudo do meio no INES. A metodologia utilizada foi a pesquisa em campo para identificar os insetos que habitam o INES. Essa sequência didática proporcionou a escrita de um texto coletivo, a vivência de uma prática pedagógica lúdica por meio da construção de uma maquete e a ampliação da Língua Brasileira de Sinais por meio da apresentação do texto e da maquete
Una mirada articuladora entre el anclaje y el dinamismo
Authors: Durán Ricardez Ignacio Salvador 
Published: December 2024

La presente reseña aborda los principales planteamientos del libro De la religiosidad vivida a la religiosidad bisagra. Experiencias de lo sagrado en el México contemporáneo (Juárez Huet, De la Torre y Gutiérrez, 2023). El principal objetivo es ofrecer una visión panorámica de los aportes que esta obra ofrece al campo de los estudios sobre religiones, con énfasis especial en tres aspectos: su valor como una mirada cualitativa profunda que dialoga con otras aproximaciones previas de corte cuantitativo; la manera en que clarifica la relación entre los estudios de las religiosidad popular en México y el enfoque de la religiosidad vivida; y la propuesta por incorporar la perspectiva de la religiosidad bisagra, como una herramienta heurística para analizar la articulación entre el anclaje a las viejas tradiciones y el dinamismo de las recomposiciones subjetivas. Asimismo, se ofrece una visión esquemática de la estructura del libro, sus objetivos y el contexto en el que se inserta, para brindar una primera aproximación a los lectores interesados.
Paleontologia como ferramenta pedagógica
Authors: Azevedo Hugo José Coelho Corrêa de , Silva Dafny Coutinho do Espirito Santo , Ramos Fernanda Campello Nogueira , Barros Brenno Gomes de 
Published: December 2024

Introdução. Paleontologia, a Ciência que estuda a vida pré-histórica por meio de fósseis, tem grande importância no ensino das Ciências Naturais, pois é capaz de promover a compreensão de processos evolutivos e biológicos. No entanto, é raramente abordada nos anos iniciais do Ensino Fundamental, limitando o aprendizado sobre o passado da Terra. Integrar essa Ciência ao currículo pode despertar o interesse científico e desenvolver habilidades como pensamento crítico e alfabetização científica. Objetivo. O objetivo do artigo é relatar uma experiência didática utilizando a Paleontologia para desenvolver leitura, escrita e pensamento científico em alunos do Ensino Fundamental I. Metodologia. Participaram da atividade 36 alunos de 6 a 8 anos. Eles manusearam réplicas de fósseis, levantaram hipóteses sobre a vida dos seres e registraram suas ideias por meio de desenhos e textos, seguidos de discussão coletiva. Resultados. A atividade promoveu o interesse pela Ciência, desenvolveu habilidades comunicativas e estimulou a alfabetização científica contextualizada. Conclusão. O uso de fósseis mostrou-se eficaz para integrar a Paleontologia ao desenvolvimento cognitivo e linguístico, sugerindo a expansão da abordagem para outros temas científicos.
WITHDRAWN: Development of the correlation between mitophagy-related genes and epilepsy
Authors: zheng jingxian , Wang Miao , Zhang Han , An Wen , Xu xiangping 
Published: December 2024

Abstract

The full text of this preprint has been withdrawn by the authors due to author disagreement with the posting of the preprint. Therefore, the authors do not wish this work to be cited as a reference. Questions should be directed to the corresponding author.
Phytochemical constituent analysis, antioxidative activity, and cytotoxic effects of Caulerpa lentillifera extracts inducing cell apoptosis and sub-G/G0-G1 cell cycle arrest in KON oral cancer cells
Authors: Manmuan Suwisit , Tubtimsri Sukannika , Sirirak Thanchanok , Petchsomrit Arpa 
Published: December 2024

Abstract

Background
The purpose of this study was to explore the anti-cancer properties of Caulerpa lentillifera (C. lentillifera) in order to gain insights into possible treatment approaches. Using the maceration method, which included solubilizing chemicals such as methanol, ethanol, and acetone, C. lentillifera was extracted. The chemical composition of acetone extracts of C. lentillifera was investigated and their anti-oxidative activity was evaluated.
Methods
The phytoconstituents of acetone extracts were screened using the LC-QTOF MS/MS technique, and the cytotoxic effect was assessed using the MTT test. We used Hoechst 33258 and AO double staining to investigate morphological changes and apoptosis, and DAPI and FDA double labeling to look at the nucleus and cytoplasm. Using a flow cytometer, the percentage of cell cycle arrest was calculated and the fraction of cell death was examined.
Results
The LC-QTOF MS/MS analyses results revealed ten main phytochemicals in the acetone extracts. The KON cells exhibited sensitivity to acetone, ethanol, and methanol in that order. By causing ROS-mediated cell death, concentrations of C. lentillifera methanol, ethanol, and acetone extract at IC40, IC60, and IC80 can cause apoptosis and cell death in less than a day. KON cells were prevented from advancing past the sub-G and G0-G1 stages by all three extracts. Furthermore, the extracts significantly impeded colony growth, migration, and invasion. The increase in cellular uptake was measured using the TEER test.
Conclusion
This work advances our understanding of the anti-tumor characteristics of C. lentillifera extract, which will benefit patients suffering from oral cancer.
Landslide Susceptibility Analysis in Kaski District, Nepal: Using Remote Sensing Technique with Intensive Field Verification
Authors: Pokharel Ramjee Prasad , Khanal Narendra Raj , Paudel Basanta 
Published: December 2024

Abstract

Landslide events generally occur rapidly, are frequent, and also unpredictable. The landslide phenomenon often happens in mountainous regions due to natural and anthropogenic factors, which cause massive destruction and turn into disasters, most commonly in the Nepal Himalayas. Steep slopes, events with high-intensity rainfall, deforestation, land degradation, and scouring of river banks by rivers are the major reasons for the occurrence of landslides. Therefore, identification of areas susceptible to landslide occurrences and preparedness planning could help reduce the losses of human lives and properties. This study screens landslide susceptibility in the Kaski district, Nepal, through a Remote Sensing (RS) approach with intensive site verification. The spatial datasets obtained from the analysis of satellite images and other relevant maps were analyzed through a Geographic Information System (GIS). Seven landslide trigger parameters, i.e., slope, aspect, rainfall, land use land cover, drainage density, fault line, and road distance, were taken into consideration. The results were verified using the field observation method. The result shows that about 6.83% of the area is under very high susceptibility, 25.77% under high susceptibility, and 29.6% of the area is located within the moderate landslide susceptibility zones. All these zones are at risk of landslides; therefore, effective mitigation and adaptation measures such as control of human occupation, including the construction of infrastructures and landslide control measures, need to be designed and implemented to reduce the losses and damages from landslides.
EHR Satisfaction Among Physicians: Analyzing the Influence of System Features and User Demographics
Authors: Sulley Saanie , Ndanga Memory 
Published: December 2024

Abstract

Patient care and practice management have both been enhanced through the use of integrated EHR systems in healthcare. Physician satisfaction with EHR systems varies substantially due to disparities in system features and user demographics. Objective This study investigates physician satisfaction with EHR systems, focusing on factors such as e-prescribing, CPOE, interoperability, and customization. It also investigates the relationship between physician age, medical degree type, and satisfaction levels. Methods We used the 2018–2021 National Electronic Health Records (NEHR) data from the National Center for Health Statistics (NCHS). The study included doctors utilizing EHR technology that responded to the NEHR survey. Descriptive statistics, correlation analysis, multivariate regression, and interaction analysis were employed with Python to examine the connections between EHR satisfaction and the independent variables. Results EHR satisfaction is most influenced by the frequency of e-prescribing (β = 0.414, p < 0.001), with CPOE usage also contributing (β = 0.096, p < 0.001). System interoperability had a slight, negative effect on satisfaction (β = -0.031, p < 0.001). Physicians aged 50 and above expressed lower levels of satisfaction with EHR systems than their younger counterparts. Specific EHR systems, like Epic and Cerner, were associated with higher satisfaction levels for all age groups. Conclusion This study highlights the role of specific EHR features such as e-prescribing and CPOE in boosting physician satisfaction. Considering the age factor, physician demographics significantly influence EHR satisfaction among older physicians. Implementing data sharing with a focus on usability is essential for ensuring user satisfaction amidst interoperability challenges. Applying these insights can improve the user-friendliness of EHR systems, boosting adoption and efficiency in healthcare delivery.
Decade of Carbapenemase-Producing Enterobacterales in New Caledonia: Integrative Surveillance Though Genomic, Phenotypic and Clinical approaches
Authors: Colot Julien , Bourles Alexandre , Cousin Léo , Delvallez Gauthier , Biron Antoine , Ruge Tiffany , Bonnin Rémi , Dortet Laurent , Brisse Sylvain , Palma Federica , Saliou Philippe , Objois Thibaut , Kainiu Malia , Pot Matthieu , Veyrier Frédéric , Goarant Cyrille 
Published: December 2024

Abstract

Carbapenemase-producing Enterobacterales (CPE) have been identified by the World Health Organization as global priority pathogens. The dissemination of these bacteria and outbreaks within healthcare facilities are of serious concern. This study investigated the transmission patterns and genetic characteristics of CPE isolated in New Caledonia from 2013 to 2022. The isolates were identified and characterized both phenotypically and whole-genome sequencing (WGS). In total 214 CPE were isolated: 199 non duplicate clinical isolates from 164 patients and 15 from hospital environmental surface. The most common genera in clinical samples were Enterobacter (34%) and Klebsiella (25%), with 194 isolates (98%) carrying IMP-type carbapenemase. WGS of 89 isolates revealed the dominance of the blaIMP-4 carbapenemase gene, found in 82 isolates. The blaIMP-4 was primarily predicted to be carried by IncL/M-type plasmid, found in 69% of the sequenced isolates. Our work revealed the circulation of 12 bacterial clusters with 61 strains involved in outbreaks or persistent over time. Genomic, phenotypic and clinical approaches identified 12 distinct outbreaks involving IMP producers. These results highlight the importance of studying plasmid transmission to better prevent silent spread of CPE. Ultimately, this study provides new guidelines for limiting the clinical spread of CPE in New Caledonia.
Probing the Dark Chemical Matter Against PDE4 for the Management of Psoriasis Using in-Silico and in-Vivo Approach
Authors: B Swapna. , Kotha Satvik , Selvaraj Divakar , setty S Ramachandra , Acharya Aruna 
Published: December 2024

Abstract

The potential downsides of standard treatments of Apremilast and Roflumilast include gastrointestinal side effects, the risk of mental episodes, and drug interactions, among other things. The rationale of the study is to pay attention to global efforts by describing currently underexplored chemical space regions. The objective was to investigate PDE4D inhibitors utilizing the dark chemical database. The goal was to address this by conducting a computational analysis that coupled docking with molecular dynamics, phase screening, and compound pharmacokinetics optimisation utilizing machine learning and artificial intelligence, which are key parts of drug discovery and development processes. We established the binding mode and critical residues (GLN-369, ILE-336, PHE-340, and PHE-372) involved in interactions with 1,39,353 dark chemical matter molecules out of which 15 hits were obtained and subjected to artificial intelligence (PK parameters) and MD simulations. RMSD and stable amino acids interact with four promising molecules 027230, 060628, 060576 and 085881. The ligand 085881 was found promising since it inhibits both IL-6 and TNF-alpha with IC50 of 18.41 µM and 34.43 µM respectively. In-vivo Erythema grading showed that 085881 test substances possess mild to moderate antipsoriatic action.