NMDA receptors potentiate activity‐dependent dendritic release of neuropeptides from hypothalamic neurons
Authors: Pitra Soledad , Zhang Meng , Cauley Edmund , Stern Javier E. 
Published: January 2019

Key points
Using ‘sniffer’ cell biosensors, we evaluated the effects of specific firing patterns and frequencies on activity‐dependent somatodendritic release of vasopressin from paraventricular nucleus neurones.
Somatodendritic release of vasopressin was rarely observed during continuous firing but was strengthened by clustered activity. Moreover, release evoked at any given frequency was robustly potentiated by NMDA receptor (NMDAR)‐mediated firing.
Differently from axonal release, NMDAR activation was necessary for somatodendritic release to occur at physiological firing frequencies, acting thus as a gating mechanism by which activity‐dependent release from these two neuronal compartments could be independently regulated.
The NMDAR‐mediated potentiation was independent of a specific firing pattern and was not accompanied by increased spike broadening, but correlated with higher dendritic Ca2+ levels.
Our studies provide fundamental novel information regarding stimulus–secretion coupling at somatodendritic compartments, and shed light into mechanisms by which activity‐dependent release of neuronal signals from axonal terminals and dendrites could be regulated in a spatially compartmentalized manner.
AbstractDendrites are now recognized to be active transmitting neuronal compartments subserving complex brain functions, including motor behaviours and homeostatic neurohumoral responses. Still, the precise mechanisms underlying activity‐dependent release of dendritic signals, and how dendritic release is regulated independently from axonal release, remains largely unknown. We used ‘sniffer’ biosensor cells to enable the measurement and study of activity‐dependent dendritic release of vasopressin (VP) from hypothalamic neurones in brain slices. SnifferVP responses were dose‐dependent, with a threshold detection level of 0.5 nM for VP, being thus a highly sensitive tool to detect endogenous physiological levels of the neuropeptide. Somatodendritic release of VP was rarely observed in response to a burst of action potentials fired in continuous mode, but was strengthened by clustered firing activity. Moreover, release evoked at any given frequency was robustly potentiated when firing was triggered by NMDA receptor (NMDAR) activation. Differently from axonal release, NMDAR activation was necessary for dendritic release to occur at physiological firing frequencies. Thus, we propose that NMDARs may act as a gating mechanism by which activity‐dependent release from these two neuronal compartments can be independently regulated. The NMDAR‐mediated potentiation of dendritic release was independent of a particular action potential waveform, firing pattern evoked, or a more pronounced spiked broadening, but correlated with higher dendritic Ca2+ levels. Overall, our studies provide fundamental novel information regarding stimulus–secretion coupling at neuronal dendrites, and shed light into mechanisms by which activity‐dependent release of neuronal signals from axonal terminals and dendrites can be regulated in a spatially compartmentalized manner.