Significance of a Critical Set of 11q Chromosome Aberrations for Diagnosis of MYC Negative Burkitt Lymphoma
Authors: Rymkiewicz Grzegorz , Chechlinska Magdalena , Grygalewicz Beata , Pienkowska-Grela Barbara , Blachnio Katarzyna , Bystydzienski Zbigniew , Romejko-Jarosinska Joanna , Sledz-Gawronska Beata , Zajdel Michalina , Domanska-Czyz Katarzyna , Woroniecka Renata , Sikorska-Mali Kinga , Siwicki Jan Konrad , Prochorec-Sobieszek Monika , Walewski Jan 
Published: October 2019

Abstract
Background: Burkitt lymphoma (BL) is characterized by a non-specific morphology and immunophenotype, a high proliferation rate, MYC rearrangements (MYC +), and by a simple karyotype. However, 5% of BL cases have no MYC rearrangements (MYC -) detectable by FISH. It is a matter of debate whether a true MYC (-) BL does exist.The WHO 2008 classification does not clearly define MYC (-) BL cases, and such cases are often misdiagnosed and treated as diffuse large B-cell lymphoma (DLBCL). We have previously described a provisional category of aggressive B-cell lymphoma unclassifiable (BCLU) with recurrent chromosome 11q aberrations, referred to as B-NHL(11q), with clinical, pathomorphological, and gene expression profile features typicalof BL,but MYC (-). B-NHLs(11q) carry proximal gains and telomeric losses of 11q. Karyotyping (CC) and FISH defined the gain region as dup(11)(q23q13) involving CCND1, ATM and KMT2A. As we have recently shown, BL and B-NHL(11q) express different levels of CD38 and CD16&CD56, and both have lower levels of miRNA-155, -21 and -26a than DLBCL. Here we describe a series of BL patients with a set of critical 11q aberrations and propose a diagnostic algorithm for a rapid work-up.
Methods: Within a group of 82 BL cases diagnosed and treated with the BL protocol at our institution, we identified 15 cases of B-NHL(11q) with BL features and 11q aberrations: MYC (-) in 11(male/female 10/1, median age [range] 24 [18-62]) and MYC (+) in 4 cases (male/female 3/1, median age [range] 36.5 [20-82]). In MYC (-) pts, the disease was confined to a single site in 82%, was bulky (>7 cm) in 64% with diameter >20 cm in 45% of cases. BL, BCLU and DLBCL diagnosis according to WHO 2008 classification was based on histopathological/immunohistochemical examination (HP/IHC), CC, FISH, and clinical characteristics in all pts. For the final evaluation, the flow cytometry (FCM) immunophenotype, array comparative genomic hybridization (aCGH) data, and miRNA expression was assessed on samples obtained by the fine needle aspiration biopsy (FNAB). In the B-NHL(11q) cases we identified 11q duplication, dup(11q), with an inversion (inv) of the duplicated region and a deletion of its telomeric region, referred to as critical set of 11q aberrations, as opposed to non-critical aberration set that did not involve all three changes. B-NHL(11q) cells were evaluated with the panel of antibodies by IHC (CD20/CD10/BCL6/ BCL2/MUM1/MYC/Ki-67/CD43/CD44), and by FCM with CD (19, 20, 22, 23, 52, 79β, 81, 5, 25, 38, 43, 44, 45, 16&56, 56, 52, 62L, 71, 200), FMC7, HLADR, and BCL2. All B-NHL(11q) cases were evaluated by CC, FISH (MYC, BCL2, BCL6, CCND1, ATM, KMT2A and telomeric 11q) and aCGH. The relative positions of CCND1, ATM, and KMT2A within a duplicated region on the aberrant chromosome 11 were used to identify inversions.
Results: A median follow-up of MYC (-) B-NHL(11q) pts treated with the BL regimen was 30 months, and 2-yr OS was 72% (95% CI: 45%, 99%). In 53% of B-NHL(11q) pts tingible body macrophages were less pronounced than in classic BL. All MYC (-) and MYC (+) B-NHLs(11q) cases presented the same phenotype and Ki-67 index of 100% and met the IHC criteria for BL. In MYC (-) and MYC (+) B-NHLs(11q) pts, all with a simple or less simple karyotype, we confirmed a critical or non-critical 11q aberrations in 10 and 5 pts, respectively. In 87% of pts we identified dup(11q), of two types: the larger part between 11q12.1 and 11q24.3 bands, and the smaller part between 11q22.3 and 11q24.1, with an additional multiplication of KMT2A inside the duplication region. In 13% of cases an inv without dup(11q) was detected. We found an inv of dup(11q) region in 73% of all cases, and no inv in dup(11q) in 2 MYC (+) cases only. Bulky tumors of >20 cm correlated with increased KMT2A copy number in B-NHLs(11q)cases.
Conclusions: B-NHL(11q) cases are clinically homogenous while 11q aberrations are heterogeneous. We believe that BLs MYC (-) do exist. Combination of HP/IHC with FNAB/FCM/CC/FISH is a reliable method for credible diagnosis of BLMYC (-). BLMYC (-) should only be diagnosed in cases where critical 11q aberrations and a simple karyotype are identified. BCLU(11q) or DLBCL(11q) cases should be diagnosed if there are more complex karyotypes accompanied by 11q aberrations of any type. We hypothesize that in BLMYC (-) and other aggressive B-NHL(11q), 11q aberrations may determine clinical and pathomorphological features equivalent to those resulting from MYC rearrangements.

Disclosures
Walewski: Gilead: Consultancy, Honoraria, Other: travel, accommodation; Seattle: Other: travel, accommodation; GSK/Novartis: Research Funding; Genetics: Other: travel, accommodation; Celgene: Honoraria, Other: travel, accommodation, Research Funding; Teva: Consultancy, Honoraria; Servier: Consultancy; Karyopharm: Consultancy; Boehringer Ingelheim: Consultancy; Ariad: Consultancy; Takeda: Consultancy, Honoraria, Other; Roche: Consultancy, Honoraria, Other: travel, accommodation, Research Funding; Sanofi: Honoraria, Other: travel, accommodation; Mundipharma: Consultancy, Honoraria, Research Funding; Janssen-Cilag: Consultancy.