bluuebunny/crossref_metadata_2025_split huggingface dataset:
Teaching Multimodal Texts in an ESP Class Using Interacty and Padlet
Authors: Kurniawati Arienta , Rohmah Zuliati 
Published: November 2024

Abstract: The utilization of multimodal texts and interactive technology has gained significant popularity. However, the existing research on multimodal texts for English for Specific Purposes (ESP) is less comprehensive. Therefore, the current study explores the potential of Interacty and Padlet to improve ESP teaching and learning in a virtual setting, their implementation in the classroom, and students’ outcomes following the application of the two apps. This study employed a descriptive qualitative approach through participant observation to collect data. Thematic Analysis was applied to analyse the fieldnotes and the accompanying transcribed data. The implementation of Interacty and Padlet in ESP courses has proven to be adaptable and effective tools to enhance the language learning through various interactive formats, quizzes, and games to promote meaningful activities. The overall success of the implementation suggests that the use of these platforms has the potential to enhance engagement, motivation, and comprehension in ESP classrooms while adding an element of fun to the learning process. This study adds to the existing research on online teaching by offering findings regarding the use of Interacty and Padlet in ESP which enables the instructor to promote students’ comprehension, meaningful learning, and joyful activities.

Abstrak: Penggunaan teks multimodal dan teknologi interaktif telah mendapat populariti yang ketara. Walau bagaimanapun, penyelidikan sedia ada mengenai teks multimodal untuk English for Specific Purposes (ESP) adalah kurang menyeluruh. Oleh itu, kajian semasa meneroka potensi Interacty dan Padlet untuk menambah baik pengajaran dan pembelajaran ESP dalam persekitaran maya, pelaksanaannya di dalam bilik darjah, dan hasil pelajar berikutan penggunaan kedua-dua aplikasi tersebut. Kajian ini menggunakan pendekatan kualitatif deskriptif melalui pemerhatian peserta untuk mengumpul data. Analisis Tematik telah digunakan untuk menganalisis nota lapangan dan data transkripsi yang disertakan. Pelaksanaan Interacty dan Padlet dalam kursus ESP telah terbukti sebagai alat yang boleh disesuaikan dan berkesan untuk meningkatkan pembelajaran bahasa melalui pelbagai format interaktif, kuiz, dan permainan untuk mempromosikan aktiviti yang bermakna. Kejayaan keseluruhan pelaksanaan menunjukkan bahawa penggunaan platform ini berpotensi untuk meningkatkan penglibatan, motivasi dan kefahaman dalam bilik darjah ESP sambil menambah unsur keseronokan kepada proses pembelajaran. Kajian ini menambah kepada penyelidikan sedia ada tentang pengajaran dalam talian dengan menawarkan penemuan berkenaan penggunaan Interacty dan Padlet dalam ESP yang membolehkan pengajar mempromosikan kefahaman pelajar, pembelajaran bermakna dan aktiviti yang menggembirakan.
Pengalaman Pasien Penderita Penyakit Jantung Koroner Post Percutaneous Coronary Intervention (PCI)
Authors: Nur Aldi Fatthya Rizqy , Erwin Erwin , Sari Niken Yuniar 
Published: December 2024

Penyakit Jantung Koroner (PJK) adalah penyakit jantung yang terjadi dikarenakan adanya penyempitan arteri koronaria, salah satu metode yang dapat digunakan untuk mengobati penyempitan pembuluh darah adalah dengan metode Percutaneous coronary intervention (PCI). Tujuan dari penelitian ini adalah untuk mengeksplorasi pengalaman pasien penderita penyakit jantung koroner yang telah melakukan tindakan Percutaneous coronary intervention (PCI) di RSUD Arifin Achmad. Metode: penelitian ini menggunakan desain penelitian fenomenologi Deskriptif. Data dikumpulkan melalui wawancara semi terstruktur dengan jumlah partisipan sebanyak 4 orang yang melakukan tindakan PCI di RSUD Arifin Achmad pada 1 bulan terakhir, pemilihan sampel yaitu melalui teknik purposive sampling. Teknik pengumpulan data yaitu wawancara, observasi, dan dokumentasi, penelitian ini dilakukan dari tanggal 27 November hingga 4 Desember 2023. Penelitian ini menghasilan tiga tema yaitu: (1) Pengalaman Pasien Penderita Penyakit Jantung Koroner saat Tindakan PCI (2) Kondisi Pasien Penderita Penyakit Jantung Koroner post PCI, (3) Dukungan Keluarga. Tindakan PCI cukup berpengaruh terhadap pasien baik secara psikologis maupun aktivitas, saat tindakan PCI dilakukan pasien merasa cemas, gugup, dan takut. Setelah tindakan PCI dilakukan pasien merasa menjadi lebih baik dari sebelum dilakukan tindakan PCI, yaitu dengan berkurangnya nyeri yang dirasakan, namun aktivitas yang dilakukan menjadi lebih terbatas. Oleh karena itu, dukungan keluarga sangat dibutuhkan dalam proses penyembuhan pasien setelah dilakukan tindakan PCI
Hubungan Dukungan Suami Dengan Kepatuhan Antenatal Care (ANC) Primigravida
Authors: Efendi Norfan Ani 
Published: December 2024

Antenatal Care (ANC) merupakan pelayanan kesehatan ibu hamil untuk menurunkan angka kematian ibu serta bayi di indonesia. Tujuan penelitian untuk mengetahui hubungan dukungan suami dengan kepatuhan Antenatal Care (ANC) primigravida di Rumah Sakit Bhayangkara Bondowoso tahun 2021, Metode penelitian analitik korelasional. Jumlah responden 49 ibu hamil primigravida di KIA Rumah Sakit Bhayangkara Bondowoso. Pengambilan responden menggunakan Total Sampling. Penelitian ini menggunakan uji Spearman. Hasil penelitian yang sudah dilakukan Dari 49 responden dukungan suami dalam kategori kurang terdapat 0 %, dukungan suami cukup 7 responden (14,3 %), dukungan suami baik 42 responden (85,7 %). Untuk kepatuhan ANC, 6 responden (12,2 %) tidak patuh pemeriksaan atau melakukan kunjungan ANC, 43 (87,8 %) responden patuh melakukan atau pemeriksaan ANC. Hasil uji Statistik didapatkan hasil nilai signifikan 0,007 lebih kecil dari 0,05 atau 0,01 sehingga dapat ditarik kesimpulan bahwa ada hubungan yang signifikan antara dukungan suami dengan kepatuhan pemeriksan ANC di Rumah Sakit Bhayangkara Bondowoso. Berdasarkan hasil penelitian bahwa sebagian besar suami mendukungan istrinya melakukan kunjungan Antenatal Care (ANC), dan banyak istri sudah patuh melakukan pemeriksaan ANC
Karakteristik Demografi Balita Stunting di Wilayah Lahan Basah Kecamatan Lima Puluh
Authors: Pramudita Pingkan Deni , Tampubolon Nurhannifah Rizky , Indriati Ganis 
Published: December 2024

Stunting merupakan suatu kondisi malnutrisi yang disebabkan oleh masalah gizi kronis dan infeksi yang berkelanjutan sejak 1000 hari pertama kehidupan. Stunting dapat menyebabkan seorang anak mengalami keterlambatan pertumbuhan. Tujuan penelitian ini untuk mengidentifikasi karakteristik demografi balita stunting di wilayah lahan basah. Metode: Penelitian ini merupakan penelitian kuantitatif yang bersifat deskripstif dengan pendekatan cross sectional. Jumlah sampel pada penelitian ini adalah 42 responden. Teknik pengambilan sampel pada penelitian ini adalah total sampling. Pengumpulan data menggunakan kuesioner yang dilakukan di Puskesmas Limapuluh. Analisis yang digunakan adalah analisis univariat. Hasil: Hasil penelitian yang dilakukan pada responden menunjukkan hasil bahwa karakteristik responden sebagian besar adalah orantua yang berusia < 20 tahun dan > 35 tahun, balita stunting sebagian besar tergolong pendek dengan jumlah anggota diatas 4 orang dalam satu keluarga, tingkat pendidikan rendah, pendapatan dibawah Upah Minimum Regional (UMR), responden sebagian besar bekerja, sanitasi terstandar dan keadaan lingkungan memenuhi syarat. Kesimpulan: Sebagian besar masalah stunting di pengaruhi oleh usia orangtua yang beresiko, jumlah anggota keluarga yang terlalu banyak dan pengasilan yang rendah.
Enhancement of biocompatibility of anodic nanotube structures on biomedical Ti–6Al–4V alloy via ultrathin TiO2 coatings
Authors: Sepúlveda Marcela , Capek Jan , Baishya Kaushik , Rodriguez-Pereira Jhonatan , Bacova Jana , Jelinkova Stepanka , Zazpe Raul , Sopha Hanna , Rousar Tomas , Macak Jan M. 
Published: December 2024

This work aims to describe the effect of the surface modification of TiO2 nanotube (TNT) layers on Ti-6Al-4V (TiAlV) alloy by ultrathin TiO2 coatings prepared via Atomic Layer Deposition (ALD) on the growth of MG-63 osteoblastic cells. The TNT layers with two distinctly different inner diameters, namely ∼15 nm and ∼50 nm, were prepared via anodic oxidation of the TiAlV alloy. Flat, i.e., non-anodized, TiAlV alloy foils were used as reference substrates. Additionally, a part of the TNT layers and alloy foils was coated with ultrathin coatings of TiO2 by ALD. The number of TiO2 ALD cycles used was 1 and 5 leading to a nominal TiO2 thickness of ∼0.055 and ∼0.3 nm, respectively. The ultrathin TiO2 coating by ALD enabled to optimize the surface hydrophilicity for optimal cell growth. In addition, coatings shaded impurities of V- and F-based species (stemming from the alloy and the anodization electrolyte) that affect the biocompatibility of the tested materials while preserving the original structure and morphology. The evaluation of the biocompatibility before and after TiO2 ALD coating on TiAlV flat surfaces and TNT layers was carried out using MG-63 osteoblastic cells and compared after incubation for up to 96 h. The cell growth, adhesion, and proliferation of the MG-63 on TiAlV foils and TNT layers showed significant enhancement after the surface modification by TiO2 ALD.
Intravenous immunoglobulin therapy: usage patterns and response to treatment in Qatar over ten years
Authors: Taha Salma A. , Thalappil Sherin , Ali Ramzy M. , Fatima Haajra , Imameldin Asaad Omer A. , Aqel Sami , Abdelaal Ahmed M. , Siepmann Timo , Barlinn Jessica , Al-Nesf Maryam A. 
Published: December 2024

BackgroundIVIg is a blood-derived antibody product initially designed as a replacement therapy in inborn errors of immunity (IEIs). However, over the last 50 years, IVIg has been used to treat a growing range of autoimmune, autoinflammatory, and secondary immunodeficiency disorders. The US FDA has licensed IVIg for use in the treatment of nine clinical indications; although, IVIg global usage extends to off-label indications with variable treatment responses. Data from Qatar on the use of IVIg is scarce; thus, hampering the formulation of local policies. This study aimed to examine the utilization patterns, clinical indications, and safety profile of IVIg usage in Qatar; a nation with a predominantly young population, and to investigate the response rates to short- and long-term IVIg treatment, as well as explore associations between age at first IVIg dose, clinical indication, and treatment response.MethodsA retrospective chart review was conducted of patients who received IVIg between March 2009, and March 2019, in Hamad General Hospital, Qatar. Demographics, immediate adverse effects of IVIg, and treatment response were collected. IVIg clinical indications were categorized into FDA- and/or EMA-approved, those supported by international guidelines; those approved as second-line therapy, and those with low or no supportive evidence.ResultsIVIg was used for 63 indications during the 10-years. The age of patients skewed towards a younger demographic (median (IQR) 24 (44-6) years); however, no significant differences in response to short- and long-term treatment between age groups were observed. Of the 841 patients, 62% received IVIg in concordance with international recommendations, while 14% bestowed the treatment for indications with low or no supportive evidence. Immediate IVIg adverse effects were documented in 4% of patients in all of the infusions received, with headaches being the most prevalent (1.8%). Variable treatment responses were observed, with the highest recovery reported in immune thrombocytopenic purpura (35%), followed by transverse myelitis (28%).ConclusionThis study provided crucial insights into IVIg utilization, safety, and treatment outcomes in Qatar’s young population. Despite variability in treatment responses and off-label use, adherence to international recommendations remained eminent. Further research is warranted to inform local guidelines and optimize IVIg therapy outcomes.
PD-L2 act as an independent immune checkpoint in colorectal cancer beyond PD-L1
Authors: Zhu Lvyun , Qu Ying , Yang Junru , Shao Tong , Kuang Jingyu , Liu Chuanyang , Qi Yanhua , Li Ming , Li Yingying , Zhang Sujuan , Wang Jingyang , Liu Yu , Liu Jiali , Hu Yanming , Zhu Lingyun , Hou Tao 
Published: December 2024

IntroductionImmunotherapy, especially immune checkpoint blockade (ICB), holds promise as a therapeutic strategy in colorectal cancer (CRC) by harnessing the patient’s immune system to target malignant cells. Particularly, the PD-1/PD-L1 axis is widely recognized for its critical role in tumor microenvironment immunosuppression. Antibodies targeting PD-1 or PD-L1 have shown sustained efficacy against various cancers, including CRC. Nonetheless, many CRC patients exhibit limited responses to such immunotherapy, and the resistance mechanisms remain incompletely understood.MethodsWe conducted experiments with C57BL/6 mice, and used the MC38 cell line for ICB treatment studies in syngeneic mouse models. Gene and protein analyses were performed using qPCR, Western Blot, and flow cytometry, with bioinformatics for clinical data survival analysis.ResultsIn this study, we reveal that targeting PD-L2 emerges as a complementary therapeutic strategy to PD-1/PD-L1 blockade in CRC. Although PD-L2 is also inducible by IFNγ, like PD-L1, it displays a unique spatial distribution within the tumor microenvironment, implying discrete roles in immune evasion. Additionally, we uncovered a significant correlation between PD-L1 and PD-L2 expression levels and the infiltration of various immune cells, encompassing multiple dendritic cell (DC) subtypes. This correlation implies an enhanced antigen presentation process that may be unleashed by blocking these two immune checkpoints.DiscussionOur results highlight the significance of PD-L2 as an essential immune checkpoint alongside PD-L1 and emphasize its potential as a target for bolstering antitumor immunity in colorectal cancer.
Kir6.1, a component of an ATP-sensitive potassium channel, regulates natural killer cell development
Authors: Samper Natalie , Hardardottir Lilja , Depierreux Delphine M. , Song Soomin C. , Nakazawa Ayano , Gando Ivan , Nakamura Tomoe Y. , Sharkey Andrew M. , Nowosad Carla R. , Feske Stefan , Colucci Francesco , Coetzee William A. 
Published: December 2024

IntroductionInvolved in immunity and reproduction, natural killer (NK) cells offer opportunities to develop new immunotherapies to treat infections and cancer or to alleviate pregnancy complications. Most current strategies use cytokines or antibodies to enhance NK-cell function, but none use ion channel modulators, which are widely used in clinical practice to treat hypertension, diabetes, epilepsy, and other conditions. Little is known about ion channels in NK cells. ResultsWe show that Kcnj8, which codes for the Kir6.1 subunit of a certain type of ATP-sensitive potassium (KATP) channel, is highly expressed in murine splenic and uterine NK cells compared to other K+ channels previously identified in NK cells. Kcnj8 expression is highest in the most mature subset of splenic NK cells (CD27-/CD11b+) and in NKG2A+ or Ly49C/I+ educated uterine NK cells. Using patch clamping, we show that a subset of NK cells expresses a current sensitive to the Kir6.1 blocker PNU-37883A. Kcnj8 does not participate in NK cell degranulation in response to tumor cells in vitro or rejection of tumor cells in vivo, or IFN-γ release. Transcriptomics show that genes previously implicated in NK cell development are amongst those differentially expressed in CD27-/CD11b+ NK cells deficient for Kcnj8. Indeed, we found that mice with NK-cell specific Kcnj8 gene ablation have fewer CD27-/CD11b+ and KLRG-1+ NK cells in the bone barrow and spleen. DiscussionThese results show that the KATP subunit Kir6.1 has a key role in NK-cell development.
Cryoablation of primary breast cancer tumors induces a systemic abscopal effect altering TIME (Tumor Immune Microenvironment) in distant tumors
Authors: Sardela de Miranda Flávia , Martinez-Marin Dalia , Babcock Rachel L. , Castro Maribel , Boligala Geetha P. , Khan Sonia Y. , Furr Kathryn L. , Castro-Piedras Isabel , Wagner Nicholas , Robison Dakota E. , Daniele Karla , Singh Sharda P. , Pruitt Kevin , Melkus Michael W. , Layeequr Rahman Rakhshanda 
Published: December 2024

IntroductionDespite recent advances, triple-negative breast cancer (TNBC) patients remain at high risk for recurrence and metastasis, which creates the need for innovative therapeutic approaches to improve patient outcomes. Cryoablation is a promising, less invasive alternative to surgical resection, capable of inducing tumor necrosis via freeze/thaw cycles. Necrotic cell death results in increased inflammatory signals and release of preserved tumor antigens, which have the potential to boost the local and systemic anti-tumor immune response. Thus, compared to surgery, cryoablation enhances the activation of T cells leading to an improved abscopal effect, defined as the occurrence of a systemic response after local treatment. We previously showed with a bilateral-tumor mouse model of TNBC that cryoablation of the primary tumor leads to increased infiltration of distant (abscopal) tumors by tumor infiltrating lymphocytes (TILs) and decreased rates of recurrence and metastasis. However, the early drivers of the cryoablation generated abscopal effect are still unknown and knowledge of the mechanism could provide insight into improving the anti-tumor immune response through pharmacologic immune modulation in addition to cryoablation.MethodsOne million 4T1-12B-luciferase expressing cells were transplanted into the mammary fat pad of BALB/c mice. Two weeks later, left (primary) tumors were either resected or cryoablated. A week after the procedure, right (abscopal) and left tumors, along with spleen, tumor-draining lymph node and blood were collected and processed for flow cytometry and/or RNA-sequencing and immunofluorescence.ResultsHere we show that cryoablation of mouse mammary carcinomas results in smaller abscopal tumors that harbor increased frequencies of anti-tumor cells [such as natural killer (NK) cells], accompanied by a systemic increase in the frequency of migratory conventional type 1 dendritic cells (cDC1; CD103+ XCR1+), compared to resection. The changes in cell frequencies are mirrored by the immune gene signature of the abscopal tumors, with cryoablation inducing genes involved with NK cell activation and leukocyte-mediated toxicity, including IL11ra1 and Pfr1.ConclusionsThese results better define the early mechanisms through which cryoablation improves tumor elimination, which is mediated by enhanced frequencies of anti-tumoral cells such as NK and cDC1s at the abscopal tumor and in the spleen of mice treated with cryoablation, respectively.
Circulating immune landscape in melanoma patients undergoing anti-PD1 therapy reveals key immune features according to clinical response to treatment
Authors: Sosa Cuevas Eleonora , Mouret Stéphane , Vayssière Guillaume , Kerboua Siham , Girard Pauline , Molens Jean-Paul , Manceau Marc , Charles Julie , Saas Philippe , Aspord Caroline 
Published: December 2024

IntroductionImmune checkpoint blockers (ICB) bring unprecedented clinical success, yet many patients endure immune mediated adverse effects and/or fail to respond. Predictive signatures of response to ICB and mechanisms of clinical efficacy or failure remain understudied. DC subsets, in network with conventional αβ T (Tconv), NK, γδ T and iNKT cells, harbor pivotal roles in tumor control, yet their involvement in response to ICB remained underexplored.MethodsWe performed an extensive longitudinal monitoring of circulating immune cells from melanoma patients treated with first-line anti-PD1, before (T0) and during treatment. We assessed the phenotypic and functional features of DC and effector cells’ subsets by multi-parametric flow cytometry and ProcartaPlex® dosages.ResultsWe revealed differences according to response to treatment and modulations of patterns during treatment, highlighting a strong link between the immune landscape and the outcome of anti-PD1 therapy. Responders exhibited higher frequencies of circulating cDC1s, CD8+ T cells, and γδ2+ T cells in central memory (CM) stage. Notably, we observed a distinct remodeling of ICP expression profile, activation status and natural cytotoxicity receptor patterns of immune subsets during treatment. Anti-PD1 modulated DCs’ functionality and triggered deep changes in the functional orientation of Tconv and γδT cells.DiscussionOverall, our work provides new insights into the immunological landscape sustaining favorable clinical responses or resistance to first-line anti-PD1 therapy in melanoma patients. Such exploration participates in uncovering the mechanism of action of anti-PD1, discovering innovative predictive signatures of response, and paves the way to design pertinent combination strategies to improve patient clinical benefits in the future.
Endovascular thrombectomy: an effective and safe therapy for perioperative ischemic stroke
Authors: Wang Feng , Xu Xiaoping , Zheng Lin , Zhong Jiawei , Wang En , Liu Yang , Ke Shaofa 
Published: December 2024

BackgroundPerioperative ischemic stroke is a rare but devastating complication. Mechanical thrombectomy is a promising therapeutic method, but very little data is available on its efficacy and safety. This study aims to answer this question by comparing the clinical outcomes of perioperative and community-onset stroke patients after endovascular therapy.MethodsA retrospective cohort study was conducted on a total of 35 perioperative and 584 community-onset acute ischemic stroke patients who underwent endovascular thrombectomy at our hospital over the past 3.5 years. The recanalization rate, clinical recovery and cerebral hemorrhage within 90 days after therapy were compared between these two patient groups.ResultsEndovascular thrombectomy provided perioperative and community-onset ischemic stroke patients with comparable rates of successful reperfusion (mTICI ≥2b grade) (97.1% vs. 97.3%; p = 0. 967) and favorable functional recovery (mRS ≤ 2) (51.4% vs. 43.3%, p = 0.348), with no increase in severe intracranial hemorrhage (0% vs. 2.6 and 1.0%, for hematoma ≥30% of infarcted tissue and intraventricular hemorrhage, respectively) within 90 days. In addition, perioperative stroke patients had higher prevalence of atrial fibrillation (42.9% vs. 26.7%; p = 0.038) and intracranial cerebral artery stenosis without clear embolism (17.1% vs. 3.8%; p &lt; 0.001) than community-onset stroke patients.ConclusionEndovascular thrombectomy is an effective and safe therapeutic approach for patients with perioperative ischemic stroke, although the results need to be validated by further studies with larger populations. Atrial fibrillation and large artery stenosis may contribute to the pathogenesis of perioperative ischemic stroke.