bluuebunny/crossref_metadata_2025_split huggingface dataset:
Arabidopsis MLK3, a Plant-specific Casein Kinase 1, Negatively Regulated Flowering and Phosphorylated Histone H3 in vivo
Authors: Wang Zhen , Kang Junmei , Jia Shangang , Zhang Tiejun , Wu Zhihai , Yu Renjie , Yang Qingchuan 
Published: October 2019

Abstract
Background: Casein kinase 1 (CK1) family members are highly conserved serine/threonine kinase present in most eukaryotes with multiple biological functions. Arabidopsis MUT9-like kinases ( MLKs ) belong to a clade CK1 specific to the plant kingdom and have been implicated collectively in modulating flowering related processes. Three of the four MLKs ( MLK1/2/4 ) have been characterized, however, little is known about MLK3 , the most divergent MLKs. Results: We demonstrated that compared with wild type, mlk3 , a truncated MLK3 , flowered slightly early under long day conditions and ectopic expression of MLK3 rescued the morphological defects of mlk3 , indicating that MLK3 negatively regulates flowering. GA 3 application accelerated flowering of both wild type and mlk3 , suggesting that mlk3 had normal GA response. The recombinant MLK3-GFP was localized in the nucleus exclusively. In vitro kinase assay revealed that the nuclear protein MLK3 phosphorylated histone 3 at threonine 3 (H3T3ph). Mutation of a conserved catalytic residue (Lysine 175) abolished the kinase activity and resulted in failure to complement the early flowering phenotype of mlk3 . Interestingly, the global level of H3T3 phosphorylation in mlk3 did not differ significantly from wild type, suggesting the redundant roles of MLKs in flowering regulation. The transcriptomic analysis demonstrated that 425 genes significantly altered expression level in mlk3 relative to wild type. The mlk3 mlk4 double mutant generated by crossing mlk3 with mlk4 , a loss-of-function mutant of MLK4 showing late flowering, flowered between the two parental lines, suggesting that MLK3 played an antagonistic role to MLK4 in plant transition to flowering. Conclusions: A serine/threonine kinase encoding gene MLK3 is a casein kinase 1 specific to the plant species and represses flowering slightly. MLK3 located in nucleus catalyzes the phosphorylation of histone H3 at threonine 3 in vitro and an intact lysine residue (K175) is indispensible for the kinase activity. This study sheds new light on the delicate control of flowering by the plant-specific CK1 in Arabidopsis.
Characteristics of nocardiosis patients with different immune status from a Chinese tertiary general hospital during 8-year period
Authors: Huang Lei , Sun Liying , Yan Yan 
Published: October 2019

Abstract
Background: Nocardia is an opportunistic pathogen from environment, which is generally thought to infect immunosuppressed patients (ISPs), but recent studies showed it could also cause infections in immunocompetent patients (ICPs). Objective: To compare the clinical characteristics, patients’ outcome, Nocardia species identification and antibiotic susceptibility profiles of nocardiosis between ICPs and ISPs. Methods: The detailed clinical data were collected from all the non-repetitive nocardiosis patients during 2011 and 2018, from a tertiary general hospital in Beijing, China. Then each Nocardia isolate was identified to species level by DNA sequencing. The antibiotic susceptibility testing was performed by E-test method, and interpreted following CLSI M24 document. The clinical and microbiological characteristics between ICPs and ISPs were compared statistically. Results: A total of 23 non-repetitive nocardiosis patients with detailed clinical data were enrolled in this study. Among them, 9 were ICPs and 14 were ISPs. All the skin and soft tissue infections occurred in ICPs (33.3% versus 0%, P<0.05). Bronchiectasis occurred more frequently in ICPs (44.4% versus 21.4%), while chronic kidney diseases and co-infection with aspergillosis occurred more frequently in ISPs (35.7% versus 0%, 35.7% versus 0%, respectively), although they did not reach the statistical significance. There were no significant difference in other clinical characteristics, Nocardia species identification, and antibiotic susceptibility between ISPs and ICPs (P>0.05). Conclusion: Nocardiosis could occur in both ISPs and ICPs. Skin and soft tissue infection and bronchiectasis occurred more frequently in ICPs. Chronic kidney diseases and co-infection with aspergillosis occurred more frequently in ISPs. These characteristics should be noticed by physicians in diagnosis of nocardiosis.
Trabecular bone scores in young HIV-infected men: a matched case-control study
Authors: Kim Youn Jeong , Kang Kwi Young , Jun Yoonhee , Kim Sang Il , Ree Kim Yang 
Published: October 2019

Abstract
Background Screening for osteoporosis with dual-energy X-ray absorptiometry (DXA) is recommended for male HIV-infected patients only above the age of 50. Recently, trabecular bone score (TBS) has been introduced as a novel tool to assess bone microarchitecture using DXA of the lumbar spine. Few studies have reported TBS values in HIV-infected individuals younger than 50 years of age. This study compared TBS values in young males infected with HIV and matched controls, and investigated the associations between TBS and demographic parameters, clinical parameters, and bone mineral density (BMD) scores. Methods A cross-sectional study of BMD and TBS in HIV-infected men (n = 80) aged between 18 and 50 years and age- and sex-matched controls (n = 80) was conducted. Results The proportion of patients with low BMD (Z-score ≤−2) was significantly greater among HIV-infected patients than among matched controls (21.3% [17/80] vs. 8.8% [7/80], p = 0.027). Mean TBS values were significantly lower in HIV-infected patients than in controls (1.41 ± 0.07 vs. 1.45 ± 0.07, p = 0.008). In both groups, TBS values were positively correlated with BMD at the lumbar spine, femoral neck, and total hip (p < 0.001); however, TBS was not correlated with body mass index. In the HIV group, TBS was negatively correlated with the duration of tenofovir disoproxil fumarate(TDF) exposure (p = 0.04). Conclusion Young men infected with HIV had abnormal bone trabecular microarchitecture, as assessed by both TBS and BMD. TBS values were correlated with both BMD and the duration of TDF exposure.
Modulation of SIRT3 expression through CDK4/6 enhances the anti-cancer effect of sorafenib in hepatocellular carcinoma cells
Authors: Jo HanHee , Yoo Dayoung , Lee JongSook , Kim Seon yoo , Chung Jee-in , Ko Hae yong , Lee Misu , Yun Mijin 
Published: October 2019

Abstract
Background : Hepatocellular carcinoma (HCC) is the fifth leading cause of cancer-related deaths worldwide. The only drug currently approved for clinical use in the treatment of advanced HCC is sorafenib. However, many patients with HCC show reduced sensitivity to sorafenib during treatment. SIRT3, a member of the mammalian sirtuin family, is a tumor suppressor in certain tumor types. To date, a few studies have investigated the effects of SIRT3 on tumor prognosis and sorafenib sensitivity in patients with HCC. Thus, this study aimed to investigate the correlation between SIRT3 expression and glucose metabolism and proliferation in HCC and to discover effective compounds that increase endogenous SIRT3 modulation effect of sorafenib. Methods: To determine the correlation between SIRT3 and glucose related proteins, immunostaining was performed with liver cancer tissue using various antibodies. To investigate whether the expression of SIRT3 in HCC is related to the resistance to sorafenib, we treated sorafenib after the modulation of SIRT3 levels in HCC cell lines (overexpression in Huh7, knockdown in HepG2). We also employed PD0332991 to modulate the SIRT3 expression in HCC cell and conducted functional assays. Results: SIRT3 expression is downregulated in high glycolytic and proliferative HCC of human patients, xenograft model and HCC cell lines. We also demonstrated that SIRT3 expression was downregulated after sorafenib treatment, resulting in reduced drug sensitivity in HCC cell lines. To enhance the anti-tumor effect of sorafenib, we employed PD0332991 (CDK4/6-Rb inhibitor) based on the correlation between SIRT3 and phospho-Rb in HCC. In addition, combined treatment with sorafenib and PD0332991 showed a synergic anti-tumor effect in HCC cells. Conclusions: Taken together, our data suggest that the modulation of SIRT3 by CDK4/6 inhibition might be used for HCC therapy together with sorafenib, which, unfortunately, has limited efficacy and whose use is often associated with drug resistance.
MiR-1202 promotes cell migration and tumor metastasis in gastric cancer cells
Authors: Shang Lingyue , Wang Yingming , Li Quanlin , Xu Meidong , Niu Weixin , Xie Wenjuan , Lou Huiling , Qiao Shouyi , Hu Jianwei 
Published: October 2019

Abstract
Introduction : Tumor metastasis significantly impacts the survival rate of gastric cancer (GC) patients. Increasing evidence has suggested that numerous microRNAs (miRNAs) are associated with tumor metastasis and could be potential candidate cancer biomarkers or therapeutic targets. Materials and Methods : Early GC cases were collected and divided into two groups according to the lymphatic metastasis (LM) situation. The microarray analysis was carried out to screen out differentially expressed miRNAs in the two groups, which were further evaluated by quantitative reverse transcription polymerase chain reaction (qRT-PCR). The roles of miR-1202 on GC cells were determined through cell growth assay, cell migration assay in vitro and pulmonary metastasis assay in the mouse model. Bioinformatics analysis was used to explore the potential mechanisms of miR-1202-mediated biological effects. Results : MiR-1202 was first identified to be the most differentially expressed miRNA in GC patients with metastasis and those without metastasis. The overexpression of miR-1202 promoted GC cell migration in vivo whereas the knockdown of miR-1202 suppressed this process. However, miR-1202 did not affect GC cell growth or cell cycle distribution. The overexpression of miR-1202 promoted pulmonary metastasis and colonization after the tail-vein injection of GC cells. Pathways related to cell adhesion, collagen fibril organization, and positive regulation of mitogen-activated protein kinase (MAPK) cascade might be involved in miR-1202-mediated biological effects. Conclusion : Our results demonstrated a positive role of miR-1202 in regulating GC cell metastasis. MiR-1202 might be developed as a novel biomarker and a potential therapeutic target for GC metastasis.
Access to CKD Care in Rural Communities of India: A Qualitative Study Exploring the Barriers and Facilitators
Authors: Jafar Tazeen , Ramakrishnan Chandrika , John Oommen , Tewari Abha , Cobb Benjamin , Legido-Quigley Helena , Sungwon Yoon , Jha Vivekanand 
Published: October 2019

Abstract
Introduction Chronic kidney disease (CKD) has become a public health challenge globally, especially in lower- and middle- income countries. The implications of adverse social and economic consequences of CKD are particularly grave in a populous country like India where CKD risk factors like diabetes and hypertension are widely prevalent. Although with early detection and timely initiation of interventions CKD progression can be slowed, huge knowledge-practice gap exists. Moreover, factors that influence access to CKD care at the community level have not been studied previously. This study aimed to explore the experiences and views of key stakeholders to identify factors that influence access to CKD care in rural India. We also sought to discern the current practices and preparedness for CKD, understand the facilitators and barriers to CKD care, and feasibility and acceptability of mobile-technology based clinical decision support system (mCDSS) for CKD care in primary care.Methods Using 15 in-depth interviews and one focus group discussion (n=6), we aimed to explore the experiences and views of different stakeholdres from primary healthcare system in rural India. We employed Lévesque’s framework for access to care, and inductive and deductive approaches in the analysis. The interviews were audio-recorded and transcribed verbatim. Using QSR nVivio 11, coding and thematic analysis was undertaken.Results Our study identified barriers in relation to access to CKD care in rural India. Fore most among them was poor knowledge and lack of awareness to CKD among patients as well as primary care physicians. Health system-level barriers like shortages of skilled healthcare professionals and medicines, fragmented referrals pathways with inadequate follow up care were identified. Increasing awareness of CKD among healthcare providers and patients, provision of CKD related supplies, and a system-level approach to care coordination were key facilitators.Conclusions Lack of awareness and knowledge on CKD among primary care providers and patients, and unprepared primary care infrastructure are key barriers for access to CKD care in rural India. There is an urgent need to raise CKD awareness among primary care physicians and patients, improve supplies for diagnostics and medications, and create efficient referral pathways for CKD in primary care.
Physiological Contributors to Early Season Whole‐Crop Vigor in Cotton
Authors: Virk Gurpreet , Snider John L , Pilon Cristiane 
Published: October 2019

ABSTRACTCultivar and early growth conditions are known to influence seedling vigor in cotton (Gossypium hirsutumL. andGossypium barbadenseL.). What has not been explored fully is the relative importance of physiological contributors to early season, whole‐crop growth under field conditions. It was hypothesized that variability in early season crop growth will be associated, to varying degrees, with leaf area development and whole‐canopy net assimilation rate (NAR). To test this hypothesis, three cultivars with anticipated differences in seedling vigor were planted in 2017 and 2018 on three different dates each year to generate differences in early season crop growth. For crop growth rate (CGR) and leaf area index (LAI) there was a significant interaction between cultivar and planting date in both years. In 2017, only planting date significantly affected NAR, but in 2018, planting date and cultivar main effects were observed for NAR. When considered across both years, variability in early crop growth, whether driven by cultivar or planting date, was strongly and positively associated with LAI, but only weakly associated with NAR. High early season LAI and CGR were also positively correlated with leaf area per plant but not leaf area ratio, specific leaf area, or leaf fraction. Thus, vigorous whole‐crop growth in the early season is more dependent on leaf area development than specific leaf activity.
Spatial Fingerprinting of Biogenic and Anthropogenic Volatile Organic Compounds in an Arid Unsaturated Zone
Authors: Green Christopher T. , Luo Wentai , Conaway Christopher H. , Haase Karl B. , Baker Ronald J. , Andraski Brian J. 
Published: October 2019

Core Ideas
17 years of VOC data show variations around and far from a mixed‐waste landfill.
Principal components analysis fingerprints landfill and ambient VOCs.
Biogenic VOCs occur throughout the deep unsaturated zone and outnumber landfill VOCs.
Anthropogenic and biogenic sources at a single site complicate analysis of VOC transport.
Subsurface volatile organic compounds (VOCs) can pose risks to human and environmental health and mediate biological processes. Volatile organic compounds have both anthropogenic and biogenic origins, but the relative importance of these sources has not been explored in subsurface environments. This study synthesized 17 yr of VOC data from the Amargosa Desert Research Site in Nevada with the goal of improving understanding of spatial and temporal variations that distinguish sources of VOCs from a landfill and from ambient sources including biogenic VOCs (bVOCs). Gas samples were collected from 1999 to 2016 from an array of shallow sample points (0.5‐ and 1.5‐m depth) and from vertical profiles at three deep boreholes: two (109 m deep) near the border of a waste facility (33 and 100 m distant), and one (29 m deep) in a remote area 3 km to the south. Samples were analyzed for target VOCs and a subset was analyzed for non‐target VOCs to enumerate a greater variety of potential bVOCs. Principal components analysis of the target and non‐target VOCs provided an assessment of the spatial variability of VOCs originating from the landfill site and from ambient sources. Ambient VOCs occurred at all sample sites across a range of depths, and most were consistent with biogenic origins, indicating for the first time the presence of bVOCs in the deep unsaturated zone. Because some VOCs have both anthropogenic and biogenic sources, discrimination of sources can be important for estimating the extent and migration of anthropogenic plumes in arid unsaturated zones.
Anti-Diabetic and Angio-Protective Effect of Guluronic Acid (G2013) as a New Nonsteroidal Anti-Inflammatory Drug in the Experimental Model of Diabetes
Authors: Mortazavi-Jahromi Seyed S. , Alizadeh Shahab , Javanbakht Mohammad H. , Mirshafiey Abbas 
Published: October 2019

Background:
This study aimed to investigate the effects of guluronic acid (G2013) on blood
sugar, insulin, and gene expression profile of oxLDL receptors (SR-A, CD36, LOX-1, and CD68) in
the experimental model of diabetes.

Methods:
18 Sprague Dawley rats were randomly assigned to three groups of healthy control, diabetic
control, and G2013 group. Diabetes was induced through intraperitoneal (IP) injection of 60 mg/kg
streptozotocin. The subjects were IP treated with 25 mg/kg of G2013 per day for 28 days. The body
weight, food intake, fasting blood glucose and insulin were measured. In addition, the expression of
mentioned genes was investigated through quantitative real-time PCR.

Results:
The data showed that the final weight increased significantly in the G2013-treated subjects
compared to the diabetic control (p < 0.05). The results indicated that final food intake significantly
reduced in the G2013-treated subjects compared to the diabetic control (p < 0.05). The study findings
also suggested that the final fasting blood glucose significantly reduced in the G2013-treated group,
whereas the final fasting serum insulin level significantly increased in this group compared to the diabetic
control (p < 0.05). Moreover, the gene expression levels of SR-A, CD36, LOX-1, and CD68 in
the G2013 group significantly reduced compared to the diabetic control (p < 0.05).

Conclusion:
This study showed that G2013, could reduce blood glucose and increase insulin levels
and reduce the gene expression level of oxLDL receptors. In addition, it may probably play an important
role in reducing the severity of diabetes-induced inflammatory symptoms.
Trends and Visibility of “Digital Health” as a Keyword in Articles by JMIR Publications in the New Millennium: Bibliographic-Bibliometric Analysis
Authors: Ahmadvand Alireza , Kavanagh David , Clark Michele , Drennan Judy , Nissen Lisa 
Published: October 2019

Background
Digital health has become an advancing phenomenon in the health care systems of modern societies. Over the past two decades, various digital health options, technologies, and innovations have been introduced; many of them are still being investigated and evaluated by researchers all around the globe. However, the actual trends and visibility of peer-reviewed publications using “digital health” as a keyword to reflect the topic, published by major relevant journals, still remain to be quantified.

Objective
This study aimed to conduct a bibliographic-bibliometric analysis on articles published in JMIR Publications journals that used “digital health” as a keyword. We evaluated the trends, topics, and citations of these research publications to identify the important share and contribution of JMIR Publications journals in publishing articles on digital health.

Methods
All JMIR Publications journals were searched to find articles in English, published between January 2000 and August 2019, in which the authors focused on, utilized, or discussed digital health in their study and used “digital health” as a keyword. In addition, a bibliographic-bibliometric analysis was conducted using the freely available Profiles Research Networking Software by the Harvard Clinical and Translational Science Center.

Results
Out of 1797 articles having “digital health” as a keyword, published mostly between 2016 and 2019, 277 articles (32.3%) were published by JMIR Publications journals, mainly in the Journal of Medical Internet Research. The most frequently used keyword for the topic was “mHealth.” The average number of times an article had been cited, including self-citations, was above 2.8.

Conclusions
The reflection of “digital health” as a keyword in JMIR Publications journals has increased noticeably over the past few years. To maintain this momentum, more regular bibliographic and bibliometric analyses will be needed. This would encourage authors to consider publishing their articles in relevant, high-visibility journals and help these journals expand their supportive publication policies and become more inclusive of digital health.
THE RELATIONSHIP BETWEEN COURTS AND MEDIA
Authors: Dimovski Darko , Popović Knežević Iva , Vujičić Miša 
Published: October 2019

Judicial authorities have the difficult task to deal with the undermined public confidence in the judiciary and to reverse this trend in favor of a general appreciation of their work by citizens. Announced judicial reform which failed, the internal problems of the judiciary, as well as tabloidization by the media that create an unfavorable environment are the causes that have led to people doubting the achievements of justice and fairness conducted by the judicial authorities. Representatives of the judiciary can change such an image only with an active, not a passive attitude towards this problem. Reticence of the judiciary is not a good way to change the unfavorable picture of their work to the desired level. Consistent implementation of the principles of the publicity, demystification of the work of the judiciary, openness towards the media, is the path that leads to the establishment of trust in the work of the judiciary. Constant communication between representatives of the judiciary and journalists, in which it eliminates any doubt that the prosecution and the courts "are hiding something" is a necessity, not just a requirement. In particular, the authors point to the delicate boundary between the justified public's interests in knowing and the abuse of freedom of expression "up to the line" through which the right of the others would be violated. In this paper we point out the causes of the unfavorable environment in question, as well as the obstacles in the communication of courts and media that occur in the everyday work of both.
A ORIGEM DO ESTRANHAMENTO: PROVOCAÇÕES INTERTEXTUAIS EM EU, TITUBA, FEITICEIRA... NEGRA DE SALEM
Authors: Mora Arthur Katrein 
Published: October 2019

Este trabalho tem por objetivo investigar a origem do estranhamento provocado pela leitura de Eu, Tituba, feiticeira... Negra de Salem (1986), de Maryse Condé. Ambientada no século XVII, o livro trata de Tituba, negra natural da ilha de Barbados e escravizada na América do Norte. Conforme as teorias Leonor Arfuch (2010), Ingedore Koch (2012) e Ritchie Robertson (2009), a análise revela que Eu, Tituba... é, estilisticamente, uma espécie de mock-epic pós-moderno, projetada com o intuito consciente de desafiar os códigos de expectativa do receptor. Uma performance poética que celebra a cultura e a espiritualidade caribenhas, com fortes elementos de fantasia, interpelando temas como racismo, misoginia, a opressão das estruturas coloniais e o apagamento do negro da história.