bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inertial Measurement Unit-Based Estimation of Foot Trajectory for Clinical Gait Analysis
Authors: Ono Yumi , Hori Koyu , Ora Hiroki , Hirobe Yuki , Mao Yufeng , Sawada Hiroyuki , Inaba Akira , Orimo Satoshi , Miyake Yoshihiro 
Published: April 2019

AbstractGait analysis is used widely in clinical practice for the evaluation of abnormal gait caused by disease. Conventionally, medical professionals use motion capture systems or make visual observations to evaluate a patient’s gait. Recent biomedical engineering studies have proposed easy-to-use gait analysis methods involving wearable sensors with inertial measurement units (IMUs). IMUs placed on the shanks just above the ankles allow for the long-term monitoring of gait because the participant can walk with or without shoes during the analysis. As far as the authors know, there is no report of the gait analysis method that estimates stride length, gait speed, stride duration, stance duration, and swing duration at the same time. In this study, we tested a proposed gait analysis method that uses IMUs attached on the shanks to estimate foot trajectory and temporal gait parameters. We evaluated this proposed method by analyzing the gait of 10 able-bodied participants (mean age 23.1 years, nine men and one woman). Wearable sensors were attached to the participants’ shanks, and we measured three-axis acceleration and three-axis angular velocity with the sensors to estimate foot trajectory during walking. We compared gait parameters estimated from the foot trajectory obtained with the proposed method and those measured with a motion capture system. Mean accuracy (mean ± standard deviation) was –0.046 ± 0.026 m for stride length, –0.036 ± 0.026 m/s for gait speed, –0.002 ± 0.019 s for stride duration, –0.000 ± 0.016 s for stance duration, and –0.002 ± 0.022 s for swing duration. These results suggest that the proposed method is useful for evaluation of clinical gait parameters.
Chemogenetic activation of excitatory neurons alters hippocampal neurotransmission in a dose-dependent manner
Authors: Pati Sthitapranjya , Salvi Sonali S. , Kallianpur Mamata , Banerjee Antara , Maiti Sudipta , Clement James P. , Vaidya Vidita A. 
Published: April 2019

AbstractDesigner Receptors Exclusively Activated by Designer Drugs (DREADD)-based chemogenetic tools are extensively used to manipulate neuronal activity in a cell-type specific manner. Whole-cell patch-clamp recordings indicate membrane depolarization, coupled with increased neuronal firing rate, following administration of the DREADD ligand, Clozapine-N-Oxide (CNO) to activate the Gq-coupled DREADD, hM3Dq. Although hM3Dq has been used to enhance neuronal firing in order to manipulate diverse behaviors, often within thirty minutes to an hour post-CNO administration, the physiological effects on excitatory neurotransmission remain poorly understood. We investigated the influence of CNO-mediated hM3Dq DREADD activation on distinct aspects of hippocampal excitatory neurotransmission at the Schaffer collateral-CA1 synapse in hippocampal slices derived from mice expressing hM3Dq in Ca2+/calmodulin dependent protein kinase α (CamKIIα)-positive excitatory neurons. Our results indicate a clear dose-dependent effect on fEPSP slope, with no change noted at the lower dose of CNO (1 µM) and a significant, long-term decline in fEPSP slope observed at higher doses (5-20 µM). Further, we noted a robust theta burst stimulus (TBS) induced long-term potentiation (LTP) in the presence of the lower CNO (1 µM) dose, which was significantly attenuated at the higher CNO (20 µM) dose. Whole-cell patch clamp recording revealed both complex dose-dependent regulation of excitability, and spontaneous and evoked activity of CA1 pyramidal neurons in response to hM3Dq activation across CNO concentrations. Our data indicate that CNO-mediated activation of the hM3Dq DREADD results in dose-dependent regulation of excitatory hippocampal neurotransmission, and highlight the importance of careful interpretation of behavioral experiments involving chemogenetic manipulation.
Display of malaria transmission-blocking antigens on chimeric duck hepatitis B virus-derived virus-like particles produced inHansenula polymorpha
Authors: Wetzel David , Chan Jo-Anne , Suckow Manfred , Barbian Andreas , Weniger Michael , Jenzelewski Volker , Reiling Linda , Richards Jack S , Anderson David A , Kouskousis Betty , Palmer Catherine , Hanssen Eric , Schembecker Gerhard , Merz Juliane , Beeson James G , Piontek Michael 
Published: April 2019

1.AbstractBackgroundMalaria caused byPlasmodium falciparumis one of the major threats to human health globally. Despite huge efforts in malaria control and eradication, highly effective vaccines are urgently needed, including vaccines that can block malaria transmission. Chimeric virus-like particles (VLP) have emerged as a promising strategy to develop new malaria vaccine candidates.MethodsWe developed yeast cell lines and processes for the expression of malaria transmission-blocking vaccine candidates Pfs25 and Pfs230 as VLP and VLP were analyzed for purity, size, protein incorporation rate and expression of malaria antigens.ResultsIn this study, a novel platform for the display ofPlasmodium falciparumantigens on chimeric VLP is presented. Leading transmission-blocking vaccine candidates Pfs25 and Pfs230 were genetically fused to the small surface protein (dS) of the duck hepatitis B virus (DHBV). The resulting fusion proteins were co-expressed in recombinantHansenula polymorpha(syn.Pichia angusta, Ogataea polymorpha) strains along with the wild-type dS as the VLP scaffold protein. Through this strategy, chimeric VLP containing Pfs25 or the Pfs230-derived fragments Pfs230c or Pfs230D1M were purified. Up to 100 mg chimeric VLP were isolated from 100 g dry cell weight with a maximum protein purity of 90 % on the protein level. Expression of the Pfs230D1M construct was more efficient than Pfs230c and enabled VLP with higher purity. VLP showed reactivity with transmission-blocking antibodies and supported the surface display of the malaria antigens on the native VLP.ConclusionThe incorporation of leadingPlasmodium falciparumtransmission-blocking antigens into the dS-based VLP scaffold is a promising novel strategy for their display on nano-scaled particles. Competitive processes for efficient production and purification were established in this study.
Sex-specific transcriptomic responses to changes in the nutritional environment
Authors: Camus M. Florencia , Piper Matthew D.W. , Reuter Max 
Published: April 2019

AbstractMales and females typically pursue divergent reproductive strategies and accordingly require different dietary compositions to maximise their fitness. Here we move from identifying sex-specific optimal diets to understanding the molecular mechanisms that underlie male and female responses to dietary variation. We examine male and female gene expression on male-optimal (carbohydrate-rich) and female-optimal (protein-rich) diets. We find that the sexes share a large core of metabolic genes that are concordantly regulated in response to dietary composition. However, we also observe smaller sets of genes with divergent and opposing regulation, most notably in reproductive genes which are over-expressed on each sex’s optimal diet. Our results suggest that nutrient sensing output emanating from a shared metabolic machinery are reversed in males and females, leading to opposing diet-dependent regulation of reproduction in males and females. Further analysis and experiments suggest that this reverse regulation occurs within the IIS/TOR network.
Thyroid hormone levels associate with insulin resistance in obese women with metabolic syndrome in Saudi Arabia: A cross-sectional study
Authors: Binobead Manal Abdulaziz , Al Badr Nawal Abdullah , Al-Qahtani Wahidah Hazzaa , AlSedairy Sahar Abdulaziz , Albrahim Tarfa Ibrahim , Alhussain Maha Hussain , Aljurbua Tahani Ali , Arzoo Shaista , Al-Qahtani Wedad Saeed 
Published: April 2019

AbstractBackgroundThe obesity epidemic is a pressing global health concern, as obesity rates continue to climb worldwide. The current study was aimed mainly to evaluate the correlation between thyroid hormones and homeostatic model assessment of insulin resistance in Saudi obese women with metabolic syndrome.Methods100 obese women aged 25 to 55 years were clinically evaluated, from which 72 women were diagnosed with the metabolic syndrome and 28 without metabolic syndrome. Insulin resistance was quantified using the homeostatic model assessment of insulin resistance method and the resulting values were analyzed for association with demographic, clinical, and metabolic parameters.ResultsThis analysis revealed that body mass index, systolic blood pressure, and biochemical parameters and fasting insulin showed statistically higher levels in the group with metabolic syndrome compared to the group without metabolic syndrome. Similarly, values of waist circumference, fat ratio, cholesterol, free thyroxine, free triiodothyronine and homeostatic model assessment of insulin resistance results were higher in the group with metabolic syndrome as compared to the group without metabolic syndrome. Correlation analysis revealed positive association of thyroid-stimulating hormone with waist circumference (P=0.01), total cholesterol (P=0.002), fasting insulin (P=0.03) and homeostatic model assessment of insulin resistance results (P<0.01), and negatively associated with diastolic blood pressure (P=0.013) and age (P=0.05). Free thyroxine was positively associated with triglyceride level (P=0.003) and negatively associated with homeostatic model assessment of insulin resistance values (P=0.035) and fasting insulin. Free triiodothyronine was positively associated with body mass index (P=0.032) and waist circumference (P= 0.006) and negatively with age (P=0.004) and total cholesterol (P=0.001).Homeostatic model assessment of insulin resistance test revealed elevated level with positive association of body mass index, waist circumference, biochemical parameters and thyroid-stimulating hormone in insulin resistant obese women. Higher level of free triiodothyronine was found to be associated with low insulin sensitivity.
LightDock goes information-driven
Authors: Roel-Touris Jorge , Bonvin Alexandre M.J.J. , Jiménez-García Brian 
Published: April 2019

AbstractThe use of experimental information has been demonstrated to increase the success rate of computational macromolecular docking. Many methods use information to post-filter the simulation output while others drive the simulation based on experimental restraints, which can become problematic for more complex scenarios such as multiple binding interfaces. We present a novel two-step method for including interface information into protein docking simulations within the LightDock framework. Prior to the simulation, irrelevant regions from the receptor are excluded for sampling (filter of initial swarms) and initial ligand poses are pre-oriented based on ligand input information. We demonstrate the applicability of this approach on the new 55 cases of the Protein-Protein Docking Benchmark 5, using different amounts of information. Even with incomplete information, a significant improvement in performance is obtained compared to blind ab initio docking.The software is supported and freely available fromhttps://github.com/brianjimenez/lightdockand analysis data fromhttps://github.com/brianjimenez/lightdock_bm5.
On the energetics and stability of a minimal fish school
Authors: Li Gen , Kolomenskiy Dmitry , Liu Hao , Thiria Benjamin , Godoy-Diana Ramiro 
Published: April 2019

AbstractThe physical basis for fish schooling is examined using three-dimensional numerical simulations of a pair of swimming fish, with kinematics and geometry obtained from experimental data. Energy expenditure and efficiency are evaluated using a cost of transport function, while the effect of schooling on the stability of each swimmer is examined by probing the lateral force and the lateral and longitudinal force fluctuations. We construct full maps of the aforementioned quantities as functions of the spatial pattern of the swimming fish pair and show that both energy expenditure and stability can be invoked as possible reasons for the swimming patterns and tail-beat synchronization observed in real fish. Our results suggest that high cost of transport zones should be avoided by the fish. Wake capture may be energetically unfavorable in the absence of kinematic adjustment. We hereby hypothesize that fish may restrain from wake capturing and, instead, adopt side-to-side configuration as a conservative strategy, when the conditions of wake energy harvesting are not satisfied. To maintain a stable school configuration, compromise between propulsive efficiency and stability, as well as between school members, ought to be considered.
SSD1suppresses phenotypes induced by the lack of Elongator-dependent tRNA modifications
Authors: Xu Fu , Byström Anders S. , Johansson Marcus J.O. 
Published: April 2019

AbstractThe Elongator complex promotes formation of 5-methoxycarbonylmethyl (mcm5) and 5-carbamoylmethyl (ncm5) side-chains on uridines at the wobble position of cytosolic eukaryotic tRNAs. In all eukaryotic organisms tested to date, the inactivation of Elongator not only leads to the lack of mcm5/ncm5groups in tRNAs, but also a wide variety of phenotypes. Although the phenotypes are most likely caused by a translational defect induced by reduced functionality of the hypomodified tRNAs, the mechanism(s) underlying individual phenotypes are poorly understood. In this study, we show that the genetic background modulates the phenotypes induced by the lack of mcm5/ncm5groups inSaccharomyces cerevisiae. We show that the stress-induced growth defects of Elongator mutants are stronger in the W303 than in the closely related S288C genetic background and that the phenotypic differences are caused by the known polymorphism at the locus for the mRNA binding protein Ssd1. Moreover, the mutantssd1allele found in W303 cells is required for the reported histone H3 acetylation and telomeric gene silencing defects of Elongator mutants. The difference at theSSD1locus also partially explains why the simultaneous lack of mcm5and 2-thio groups at wobble uridines is lethal in the W303 but not in the S288C background. Collectively, our results demonstrate that theSSD1locus modulates phenotypes induced by the lack of Elongator-dependent tRNA modifications.Author SummaryModified nucleosides in the anticodon region of tRNAs are important for the efficiency and fidelity of translation. The Elongator complex promotes formation of several related modified uridine residues at the wobble position of eukaryotic tRNAs. In yeast, plants, worms, mice and humans, mutations in genes for Elongator subunits lead to a wide variety of different phenotypes. Here, we show that the genetic background modulates the phenotypic consequences of the inactivation of budding yeast Elongator. This background effect is largely a consequence of a polymorphism at theSSD1locus, encoding a RNA binding protein that influences translation, stability and/or localization of mRNAs. We show that several phenotypes reported for yeast Elongator mutants are either significantly stronger or only detectable in strains harboring a mutantssd1allele. Thus,SSD1is a suppressor of the phenotypes induced by the hypomodification of tRNAs.
The effects of work experience on interpretations of recruitment advertisements and organizational attraction
Authors: Yockey Mark 
Published: April 2019

PurposeThe purpose of this paper is to investigate potential differences in experienced and inexperienced workers’ interpretations of recruitment ads.Design/methodology/approachThe paper uses a between subjects design to compare responses to recruitment advertisements. The advertisements varied in terms of compensation information.FindingsWork experience did not alter perceptions of organizational culture but it did affect levels of organizational attraction. The implication is that all workers interpret recruitment advertisements in a similar manner but more experienced workers prefer different work environments than less experienced workers.Research limitations/implicationsThe results generally support the use of student populations or inexperienced workers in recruitment research. The study was limited to perceptions of pay statements. Other forms of recruitment information needs to be investigated.Practical implicationsCompanies seeking to recruit experienced workers need to be attentive to how those workers will view the company’s culture based on information in their recruitment advertisements.Originality/valueThis study is one of a very limited number of organizational attraction studies comparing experienced and inexperienced workers. It is important because it helps clarify the underlying mechanisms impacting organizational attraction based on work experience.
User adoption of a hybrid social tagging approach in an online knowledge community
Authors: Qin Chunxiu , Liu Yaxi , Mou Jian , Chen Jiangping 
Published: April 2019

Purpose
Online knowledge communities make great contributions to global knowledge sharing and innovation. Resource tagging approaches have been widely adopted in such communities to describe, annotate and organize knowledge resources mainly through users’ participation. However, it is unclear what causes the adoption of a particular resource tagging approach. The purpose of this paper is to identify factors that drive users to use a hybrid social tagging approach.

Design/methodology/approach
Technology acceptance model and social cognitive theory are adopted to support an integrated model proposed in this paper. Zhihu, one of the most popular online knowledge communities in China, is taken as the survey context. A survey was conducted with a questionnaire and collected data were analyzed through structural equation model.

Findings
A new hybrid social resource tagging approach was refined and described. The empirical results revealed that self-efficacy, perceived usefulness (PU) and perceived ease of use exert positive effect on users’ attitude. Moreover, social influence, PU and attitude impact significantly on users’ intention to use a hybrid social resource tagging approach.

Originality/value
Theoretically, this study enriches the type of resource tagging approaches and recognizes factors influencing user adoption to use it. Regarding the practical parts, the results provide online information system providers and designers with referential strategies to improve the performance of the current tagging approaches and promote them.
Single voxel approach for Lorentz force evaluation
Authors: Dölker Eva-Maria , Petković Bojana , Schmidt Reinhard , Ziolkowski Marek , Brauer Hartmut , Haueisen Jens 
Published: April 2019

Purpose
Lorentz force evaluation is a non-destructive evaluation method for conducting specimens. The movement of a specimen relative to a permanent magnet leads to Lorentz forces that are perturbed in the presence of a defect. This defect response signal (DRS) is used for defect reconstruction. To solve a linear inverse problem for defect reconstruction, an accurate and fast forward computation method is required. As existing forward methods are either too slow or too inaccurate, the purpose of this paper is to propose the single voxel approach (SVA) as a novel method.

Design/methodology/approach
In SVA, the DRS is computed as a superposition of DRSs from single defect voxels, which are calculated in advance, by applying the boundary element source method. This research uses a setup of an isotropic conducting specimen, a spherical permanent magnet and defects of different shapes at different depths. With the help of simulations, this study compares the SVA to the previously proposed approximate forward solution (AFS) and the extended area approach (EAA) using the normalized root mean square error (NRMSE). Simulated data using the finite element method serve as the reference solution.

Findings
SVA shows across all simulations NRMSE values <2.5 per cent compared to <8 per cent for EAA and <12 per cent for AFS.

Originality/value
The superposition principle of SVA allows for the application of linear inverse methods for defect reconstruction while providing sufficient accuracy of the forward method.
Employee deviant behavior: role of culture and organizational relevant support
Authors: Kalemci R. Arzu , Kalemci-Tuzun Ipek , Ozkan-Canbolat Ela 
Published: April 2019

PurposeThe purpose of this paper is to increase the knowledge and understanding of organizational and supervisory support in the context of employee deviant workplace behavior (DWB) by examining the potential associations of employees’ cultural value orientations. This paper aims to: clarify DWB; review perceived organizational support (POS) and perceived supervisory support (PSS); discuss the meaning of employees’ cultural value orientations (individualism–collectivism, power distance and paternalism); use the fuzzy logic model to analyze relationships between DWB and POS, as well as PSS and employees’ cultural value orientations.Design/methodology/approachThis research applies a fuzzy-set qualitative comparative analysis.FindingsThe results show the role of employee perceived organizational and supervisory support and cultural dimension (power distance and paternalism) configurations on employee DWB.Originality/valueThe main originality of this study is to further increase the understanding of organizational and supervisory support in the context of employee DWB by examining the potential associations of employees’ cultural value orientations. This study extends the previous research by providing evidence that organizational and supervisory support influences employees’ DWB.
Common and uncommon imaging features of abdominal tuberculosis
Authors: Gupta Pankaj , Kumar Suresh , Sharma Vishal , Mandavdhare Harshal , Dhaka Narender , Sinha Saroj K , Dutta Usha , Kochhar Rakesh 
Published: April 2019

SummaryDespite the advances in the medical care, tuberculosis (TB) still remains an important health problem. This is particularly relevant to the developing countries as well as the immunocompromised population in the developed world. Multidrug resistance poses another challenge and may be responsible for increasing incidence of TB, to some extent. The respiratory system is the most commonly involved, although any organ system may be affected. Abdominal involvement occurs in 11–12% of the patients with extrapulmonary TB. The clinical features of abdominal TB are nonspecific. Imaging plays an important role in the diagnosis of abdominal TB. Although a few imaging features strongly favour the possibility of TB, abdominal TB is a greater masquerader. In this review, we highlight the entire spectrum of the manifestations of abdominal tuberculosis (excluding the genitourinary involvement) with an emphasis on imaging findings.