bluuebunny/crossref_metadata_2025_split huggingface dataset:
EIF5A1 promotes trophoblast migration and invasion via ARAF-mediated activation of the integrin/ERK signaling pathway
Authors: Zhang Jing , Mo Hui-Qin , Tian Fu-Ju , Zeng Wei-Hong , Liu Xiao-Rui , Ma Xiao-Ling , Li Xiao , Qin Shi , Fan Cui-Fang , Lin Yi 
Published: September 2018

AbstractTrophoblast dysfunction is one mechanism implicated in the etiology of recurrent miscarriage (RM). Regulation of trophoblast function, however, is complex and the mechanisms contributing to dysregulation remain to be elucidated. Herein, we found EIF5A1 expression levels to be significantly decreased in cytotrophoblasts in RM villous tissues compared with healthy controls. Using the HTR-8/SVneo cell line as a model system, we found that overexpression of EIF5A1 promotes trophoblast proliferation, migration and invasion in vitro. Knockdown of EIF5A1 or inhibiting its hypusination with N1-guanyl-1,7-diaminoheptane (GC7) suppresses these activities. Similarly, mutating EIF5A1 to EIF5A1K50A to prevent hypusination abolishes its effects on proliferation, migration and invasion. Furthermore, upregulation of EIF5A1 increases the outgrowth of trophoblasts in a villous explant culture model, whereas knockdown has the opposite effect. Suppression of EIF5A1 hypusination also inhibits the outgrowth of trophoblasts in explants. Mechanistically, ARAF mediates the regulation of trophoblast migration and invasion by EIF5A1. Hypusinated EIF5A1 regulates the integrin/ERK signaling pathway via controlling the translation of ARAF. ARAF level is also downregulated in trophoblasts of RM villous tissues and expression of ARAF is positively correlated with EIF5A1. Together, our results suggest that EIF5A1 may be a regulator of trophoblast function at the maternal–fetal interface and low levels of EIF5A1 and ARAF may be associated with RM.
Gastric cancer mesenchymal stem cells derived IL-8 induces PD-L1 expression in gastric cancer cells via STAT3/mTOR-c-Myc signal axis
Authors: Sun Li , Wang Qianqian , Chen Bin , Zhao Yuanyuan , Shen Bo , Wang Hua , Xu Jing , Zhu Miaolin , Zhao Xiangdong , Xu Changgen , Chen Zhihong , Wang Mei , Xu Wenrong , Zhu Wei 
Published: September 2018

AbstractThe expression of PD-L1 in tumor cells is one of the main causes of tumor immune escape. However, the exact mechanism for regulating PD-L1 expression in gastric cancer (GC) cells remains unclear. Our previous studies have shown that mesenchymal stem cells (MSCs) exert broad immunosuppressive potential, modulating the activity of cells either in innate or adaptive immune system to promote tumor progress. This study aims to investigate whether GCMSCs regulate the PD-L1 expression in GC cells and explore the specific molecular mechanism. The results have shown that GCMSCs enhanced PD-L1 expression in GC cells resulting in the resistance of GC cells to CD8+ T cells cytotoxicity. However, this resistance was attenuated with IL-8 inhibition. Further studies proved that IL-8 derived from GCMSCs induced PD-L1 expression in GC cells via c-Myc regulated by STAT3 and mTOR signaling pathways. Our data indicated that blocking IL-8 derived from GCMSCs may overcome the immune escape induced by PD-L1 in GC cells and provide a potential strategy to enhance the immunotherapy efficiency in GC.
3D micro-environment regulates NF-κβ dependent adhesion to induce monocyte differentiation
Authors: Bhattacharya Anindita , Agarwal Mahesh , Mukherjee Rachita , Sen Prosenjit , Sinha Deepak Kumar 
Published: September 2018

AbstractDifferentiation of monocytes entails their relocation from blood to the tissue, hence accompanied by an altered physicochemical micro-environment. While the mechanism by which the biochemical make-up of the micro-environment induces differentiation is known, the fluid-like to gel-like transition in the physical micro-environment is not well understood. Monocytes maintain non-adherent state to prevent differentiation. We establish that irrespective of the chemical makeup, a 3D gel-like micro-environment induces a positive-feedback loop of adhesion-MAPK-NF-κβ activation to facilitate differentiation. In 2D fluid-like micro-environment, adhesion alone is capable of inducing differentiation via the same positive-feedback signaling. Chemical inducer treatment in fluid-like micro-environment, increases the propensity of monocyte adhesion via a brief pulse of p-MAPK. The adhesion subsequently elicit differentiation, establishing that adhesion is both necessary and sufficient to induce differentiation in 2D/3D micro-environment. MAPK, and NF-κβ being key molecules of multiple signaling pathways, we hypothesize that biochemically inert 3D gel-like micro-environment would also influence other cellular functions.
MicroRNA-128a represses chondrocyte autophagy and exacerbates knee osteoarthritis by disrupting Atg12
Authors: Lian Wei-Shiung , Ko Jih-Yang , Wu Re-Weng , Sun Yi-Chih , Chen Yu-Shan , Wu Shin-Long , Weng Lin-Hsiu , Jahr Holger , Wang Feng-Sheng 
Published: September 2018

AbstractChondrocyte loss is a prominent feature of osteoarthritis (OA). Autophagy is indispensable in maintaining the metabolic activities of cells exposed to deleterious stress. The contribution of microRNA signaling to chondrocyte autophagy in OA development remains elusive. We uncovered an association between poor autophagy and increased miR-128a expressions in articular chondrocytes of patients with end-stage knee OA and in a rat anterior cruciate ligament transection (ACLT) model for OA development. Cartilage matrix degradation and severe OA histopathology was evident upon forced miR-128a expression within the articular compartment. Intra-articular injections with miR-128a antisense oligonucleotide stabilized chondrocyte autophagy and slowed ACLT-mediated articular tissue destruction, including cartilage erosion, synovitis, osteophyte formation, and subchondral plate damage. In vitro, miR-128 signaling hindered Atg12 expression, LC3-II conversion, and autophagic puncta formation through targeting the 3′-untranslated region of Atg12. It increased apoptotic programs, diminishing cartilage formation capacity of articular chondrocytes. Inactivating histone methyltransferase EZH2 reduced methyl histone H3K27 enrichment in the miR-128a promoter and upregulated miR-128a transcription in inflamed chondrocytes. Taken together, miR-128a-induced Atg12 loss repressed chondrocyte autophagy to aggravate OA progression. EZH2 inactivation caused H3K27 hypomethylation to accelerate miR-128a actions. Interruption of miR-128a signaling attenuated chondrocyte dysfunction and delayed OA development. Our data provide new insights into how miR-128a signaling affects chondrocyte survival and articular cartilage anabolism and highlight the potential of miR-128a targeting therapy to alleviate knee OA.
A key function for microtubule-associated-protein 6 in activity-dependent stabilisation of actin filaments in dendritic spines
Authors: Peris Leticia , Bisbal Mariano , Martinez-Hernandez José , Saoudi Yasmina , Jonckheere Julie , Rolland Marta , Sebastien Muriel , Brocard Jacques , Denarier Eric , Bosc Christophe , Guerin Christophe , Gory-Fauré Sylvie , Deloulme Jean Christophe , Lanté Fabien , Arnal Isabelle , Buisson Alain , Goldberg Yves , Blanchoin Laurent , Delphin Christian , Andrieux Annie 
Published: September 2018

AbstractEmerging evidence indicates that microtubule-associated proteins (MAPs) are implicated in synaptic function; in particular, mice deficient for MAP6 exhibit striking deficits in plasticity and cognition. How MAP6 connects to plasticity mechanisms is unclear. Here, we address the possible role of this protein in dendritic spines. We find that in MAP6-deficient cortical and hippocampal neurons, maintenance of mature spines is impaired, and can be restored by expressing a stretch of the MAP6 sequence called Mc modules. Mc modules directly bind actin filaments and mediate activity-dependent stabilisation of F-actin in dendritic spines, a key event of synaptic plasticity. In vitro, Mc modules enhance actin filament nucleation and promote the formation of stable, highly ordered filament bundles. Activity-induced phosphorylation of MAP6 likely controls its transfer to the spine cytoskeleton. These results provide a molecular explanation for the role of MAP6 in cognition, enlightening the connection between cytoskeletal dysfunction, synaptic impairment and neuropsychiatric illnesses.
High niche diversity in Mesozoic pollinating lacewings
Authors: Liu Qing , Lu Xiumei , Zhang Qingqing , Chen Jun , Zheng Xiaoting , Zhang Weiwei , Liu Xingyue , Wang Bo 
Published: September 2018

AbstractNiche diversity of pollinating insects plays a vital role in maintaining extant terrestrial ecosystems. A key dimension of pollination niches refers to the insect proboscis length that commonly matches the floral tube length. Here we describe new kalligrammatid lacewings (an iconic Mesozoic pollinating insect lineage) from late Cretaceous Burmese amber and Mesozoic sediments in China. Kalligrammatids display complex configurations of elongate mouthpart elements consisting of well-developed maxillae, labium and their palps. The mouthpart lengths vary among species, from 0.6 to 18.0 mm, suggesting corresponding variability in the floral tube lengths of Mesozoic plants. With the diversification of pollinating habits, the kalligrammatids presented highly divergent traits related to chemical communication and defence mechanisms. Together with other Mesozoic long-proboscid insects, these fossils not only reveal the high niche diversity of Mesozoic pollinating insects but also highlight the diversity of Mesozoic pollinator-dependent plants prior to the rise of angiosperms.
Energy cost of entanglement extraction in complex quantum systems
Authors: Bény Cédric , Chubb Christopher T. , Farrelly Terry , Osborne Tobias J. 
Published: September 2018

AbstractWhat is the energy cost of extracting entanglement from complex quantum systems? Operationally, we may wish to actually extract entanglement. Conceptually, we may wish to physically understand the entanglement distribution as a function of energy. This is important, especially for quantum field theory vacua, which are extremely entangled. Here we build a theory to understand the energy cost of entanglement extraction. First, we consider a toy model, and then we define the entanglement temperature, relating energy cost to extracted entanglement. Next, we give a physical argument quantifying the energy cost of entanglement extraction in some quantum field vacua. There the energy cost depends on the spatial dimension: in one dimension, for example, it grows exponentially with extracted entanglement. Next, we provide approaches to bound the energy cost of extracting entanglement more generally. Finally, we look at spin chain models numerically to calculate the entanglement temperature using matrix product states.
Three-dimensional X-ray diffraction imaging of dislocations in polycrystalline metals under tensile loading
Authors: Cherukara Mathew J. , Pokharel Reeju , O’Leary Timothy S. , Baldwin J. Kevin , Maxey Evan , Cha Wonsuk , Maser Jorg , Harder Ross J. , Fensin Saryu J. , Sandberg Richard L. 
Published: September 2018

AbstractThe nucleation and propagation of dislocations is an ubiquitous process that accompanies the plastic deformation of materials. Consequently, following the first visualization of dislocations over 50 years ago with the advent of the first transmission electron microscopes, significant effort has been invested in tailoring material response through defect engineering and control. To accomplish this more effectively, the ability to identify and characterize defect structure and strain following external stimulus is vital. Here, using X-ray Bragg coherent diffraction imaging, we describe the first direct 3D X-ray imaging of the strain field surrounding a line defect within a grain of free-standing nanocrystalline material following tensile loading. By integrating the observed 3D structure into an atomistic model, we show that the measured strain field corresponds to a screw dislocation.
The shape of watersheds
Authors: Sassolas-Serrayet Timothée , Cattin Rodolphe , Ferry Matthieu 
Published: September 2018

AbstractSince the 1950s river networks have been intensely researched in geosciences and hydrology. This led to the definition of scaling laws that described the organisation of landscapes under fluvial incision and were later explored by statistical physics and fractal mathematics. The emblematic Hack’s Law proposes a power-law relationship between watershed area and main stream length. Though extensively documented, a wide range of values is still reported for Hack’s parameters. Some authors associate this dispersion to local geologic and climatic conditions. Here based on the analysis of large sets of river basins in various climatic and geological settings, we confirm the geometric similarity of river networks. We demonstrate that basin shape is mostly related to Hack’s coefficient and not to the exponent, independently of external forcing such as lithology and pluviometry.
Evidence for dynamic kagome ice
Authors: Lhotel E. , Petit S. , Ciomaga Hatnean M. , Ollivier J. , Mutka H. , Ressouche E. , Lees M. R. , Balakrishnan G. 
Published: September 2018

AbstractThe search for two-dimensional quantum spin liquids, exotic magnetic states remaining disordered down to zero temperature, has been a great challenge in frustrated magnetism over the last few decades. Recently, evidence for fractionalized excitations, called spinons, emerging from these states has been observed in kagome and triangular antiferromagnets. In contrast, quantum ferromagnetic spin liquids in two dimensions, namely quantum kagome ices, have been less investigated, yet their classical counterparts exhibit amazing properties, magnetic monopole crystals as well as magnetic fragmentation. Here, we show that applying a magnetic field to the pyrochlore oxide Nd2Zr2O7, which has been shown to develop three-dimensional quantum magnetic fragmentation in zero field, results in a dimensional reduction, creating a dynamic kagome ice state: the spin excitation spectrum determined by neutron scattering encompasses a flat mode with a six arm shape akin to the kagome ice structure factor, from which dispersive branches emerge.
Ring synthetic chromosome V SCRaMbLE
Authors: Wang Juan , Xie Ze-Xiong , Ma Yuan , Chen Xiang-Rong , Huang Yao-Qing , He Bo , Bin Jia  , Li Bing-Zhi , Yuan Ying-Jin 
Published: September 2018

AbstractStructural variations (SVs) exert important functional impacts on biological phenotypic diversity. Here we show a ring synthetic yeast chromosome V (ring_synV) can be used to continuously generate complex genomic variations and improve the production of prodeoxyviolacein (PDV) by applying Synthetic Chromosome Recombination and Modification by LoxP-mediated Evolution (SCRaMbLE) in haploid yeast cells. The SCRaMbLE of ring_synV generates aneuploid yeast strains with increased PDV productivity, and we identify aneuploid chromosome I, III, VI, XII, XIII, and ring_synV. The neochromosome of SCRaMbLEd ring_synV generated more unbalanced forms of variations, including duplication, insertions, and balanced forms of translocations and inversions than its linear form. Furthermore, of the 29 novel SVs detected, 11 prompted the PDV biosynthesis; and the deletion of uncharacterized gene YER182W is related to the improvement of the PDV. Overall, the SCRaMbLEing ring_synV embraces the evolution of the genome by modifying the chromosome number, structure, and organization, identifying targets for phenotypic comprehension.
Multi-terrane structure controls the contrasting lithospheric evolution beneath the western and central–eastern Tibetan plateau
Authors: Huangfu Pengpeng , Li Zhong-Hai , Gerya Taras , Fan Weiming , Zhang Kai-Jun , Zhang Huai , Shi Yaolin 
Published: September 2018

AbstractThe Tibetan plateau is manifested by contrasting along-strike lithospheric structures, but its formation mechanism and the relationship with the heterogeneous multi-terrane configuration is a challenging problem. Here we conduct systematic numerical modeling to explore the roles of width, density, and rheological properties of the multiple terranes in the lithospheric evolution of the Tibetan plateau, which reveals two distinct collision modes. In Mode-I, the lithospheric mantles of both the strong and weak terranes in the Tibetan plate are completely detached, followed by the underthrusting of Indian lithosphere beneath the whole plateau. Alternatively, Mode-II is characterized by full detachment of the weak terranes, but (partial) residue of the strong terranes during collision. These two contrasting modes, broadly consistent with the lithospheric structures of western and central–eastern Tibetan plateau, respectively, are strongly dependent on the along-strike variation of the width of the strong Lhasa–Qiangtang terranes.
Concyclic CH-π arrays for single-axis rotations of a bowl in a tube
Authors: Matsuno Taisuke , Fujita Masahiro , Fukunaga Kengo , Sato Sota , Isobe Hiroyuki 
Published: September 2018

AbstractThe hydrogen bond is undoubtedly one of the most important non-covalent interactions. Among the several types of the hydrogen bonds, the CH–π interaction is a relatively new notion that is being recognised in chemistry and biology. Although the CH–π hydrogen bond and conventional hydrogen bonds share common features such as directionality, this weak interaction has played a secondary role in molecular recognition. In this study, we have devised a host–guest complex that is assembled solely by the CH–π hydrogen bonds. Multivalent interactions of a bowl-shaped hydrocarbon with its peripheral hydrogen atoms are made possible via CH–π hydrogen bonds by adopting a tubular hydrocarbon as a host for their enthalpy-driven complexation. Concyclic arrays of weak hydrogen bonds further allow dynamic rotational motions of the guest in the host. Solid-state analysis with crystallographic and spectroscopic methods reveal a single-axis rotation of the bowl in the tube.
Gender differences in individual variation in academic grades fail to fit expected patterns for STEM
Authors: O’Dea R. E. , Lagisz M. , Jennions M. D. , Nakagawa S. 
Published: September 2018

AbstractFewer women than men pursue careers in science, technology, engineering and mathematics (STEM), despite girls outperforming boys at school in the relevant subjects. According to the ‘variability hypothesis’, this over-representation of males is driven by gender differences in variance; greater male variability leads to greater numbers of men who exceed the performance threshold. Here, we use recent meta-analytic advances to compare gender differences in academic grades from over 1.6 million students. In line with previous studies we find strong evidence for lower variation among girls than boys, and of higher average grades for girls. However, the gender differences in both mean and variance of grades are smaller in STEM than non-STEM subjects, suggesting that greater variability is insufficient to explain male over-representation in STEM. Simulations of these differences suggest the top 10% of a class contains equal numbers of girls and boys in STEM, but more girls in non-STEM subjects.
Rehabilitative exercise and spatially patterned nanofibrillar scaffolds enhance vascularization and innervation following volumetric muscle loss
Authors: Nakayama Karina H. , Alcazar Cynthia , Yang Guang , Quarta Marco , Paine Patrick , Doan Linda , Davies Adam , Rando Thomas A. , Huang Ngan F. 
Published: September 2018

AbstractMuscle regeneration can be permanently impaired by traumatic injuries, despite the high regenerative capacity of skeletal muscle. Implantation of engineered biomimetic scaffolds to the site of muscle ablation may serve as an attractive off-the-shelf therapeutic approach. The objective of the study was to histologically assess the therapeutic benefit of a three-dimensional spatially patterned collagen scaffold, in conjunction with rehabilitative exercise, for treatment of volumetric muscle loss. To mimic the physiologic organization of skeletal muscle, which is generally composed of myofibers aligned in parallel, three-dimensional parallel-aligned nanofibrillar collagen scaffolds were fabricated. When implanted into the ablated murine tibialis anterior muscle, the aligned nanofibrillar scaffolds, in conjunction with voluntary caged wheel exercise, significantly improved the density of perfused microvessels, in comparison to treatments of the randomly oriented nanofibrillar scaffold, decellularized scaffold, or in the untreated control group. The abundance of neuromuscular junctions was 19-fold higher when treated with aligned nanofibrillar scaffolds in conjunction with exercise, in comparison to treatment of aligned scaffold without exercise. Although, the density of de novo myofibers was not significantly improved by aligned scaffolds, regardless of exercise activity, the cross-sectional area of regenerating myofibers was increased by > 60% when treated with either aligned and randomly oriented scaffolds, in comparison to treatment of decellularized scaffold or untreated controls. These findings demonstrate that voluntary exercise improved the regenerative effect of aligned scaffolds by augmenting neurovascularization, and have important implications in the design of engineered biomimetic scaffolds for treatment of traumatic muscle injury.
Magnetic domain walls as broadband spin wave and elastic magnetisation wave emitters
Authors: Holländer Rasmus B. , Müller Cai , Schmalz Julius , Gerken Martina , McCord Jeffrey 
Published: September 2018

AbstractWe report on the direct observation of spin wave and elastic wave emission from magnetic domain walls in ferromagnetic thin films. Driven by alternating homogeneous magnetic fields the magnetic domain walls act as coherent magnetisation wave sources. Directional and low damped elastic waves below and above the ferromagnetic resonance are excited. The wave vector of the magnetoelastically induced acoustic waves is tuned by varying the excitation frequency. The occurrence of elastic wave emission is proved by a combination of micromagnetic and mechanical finite element simulations. Domain wall emitted magnetostatic surface spin waves occur at higher frequencies, which characteristics are confirmed by micromagnetic simulations. The distinct modes of magnetisation wave excitation from micromagnetic objects are a general physical phenomenon relevant for dynamic magnetisation processes in structured magnetic films. Magnetic domain walls can act as reconfigurable antennas for spin wave and elastic wave generation. The wave orientation can be controlled separately via the domain wall orientation for elastic waves and via magnetization orientation for magnetostatic surface spin waves.