bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synchronisation of Arabidopsis flowering time and whole-plant senescence in seasonal environments
Authors: Miryeganeh Matin , Yamaguchi Masaki , Kudoh Hiroshi 
Published: July 2018

AbstractSynchronisation of flowering phenology has often been observed between individuals within plant species. We expected that a critical role of flowering-time control under natural conditions is a phenological synchronisation. However, no studies have quantified the level of synchronisation of reproductive timing relative to germination timing under natural conditions. In a sequential seeding experiment (SSE) in which we manipulated the germination timing of Arabidopsis thaliana accessions, we developed a quantification index to evaluate reproductive synchrony in annual plants. In the SSE, we identified a novel phenomenon of reproductive synchrony: senescence synchrony. The role of vernalisation in realising flowering synchrony between plants of different ages under natural conditions was demonstrated by synchronisation and de-synchronisation of flowering initiation in vernalisation-sensitive and less-vernalisation-sensitive accessions, respectively. We also observed up-regulation of senescence-related genes at corresponding times. The approach we developed in this study provides a set of concepts and procedures that can be used to study reproductive synchrony experimentally under natural conditions.
Routine hemodialysis induces a decline in plasma magnesium concentration in most patients: a prospective observational cohort study
Authors: Leenders Nicoline H. J. , van Ittersum Frans J. , Hoekstra Tiny , Hoenderop Joost G. J. , Vervloet Marc G. 
Published: July 2018

AbstractIn hemodialysis patients, lower plasma magnesium (Mg) concentrations are associated with a higher overall and cardiovascular mortality. The optimal concentration appears to be above the reference range for the healthy population. Plasma Mg is not routinely measured after hemodialysis. Aim of this study was to determine the effect of routine hemodialysis on plasma Mg. Plasma Mg was measured in duplicate before (Mgpre) and after (Mgpost) dialysis in 6 consecutive hemodialysis sessions of 34 patients using a fixed 0.50 mmol/L dialysate Mg concentration. Mean Mgpre was 0.88 mmol/L (±0.14) and mean Mgpost was statistically significantly lower: mean intra-dialytic decline 0.10 mmol/L (95%-CI 0.06–0.13). A 0.10 mmol/L higher Mgpre was associated with a 0.03 mmol/L higher Mgpost (95%-CI 0.024–0.037). At a Mgpre of 0.74 mmol/L, Mgpost equalled Mgpre. There was an intra-dialytic decline of plasma Mg at higher Mgpre values and an increase at lower Mgpre values. In conclusion, in the majority of the hemodialysis patients, Mgpre concentrations are in the reference range of the healthy population, which may be too low for hemodialysis patients. Routine hemodialysis with the widely used 0.50 mmol/L dialysate Mg concentration, further declines magnesium in the majority of patients. Current dialysate Mg concentrations may be too low.
Irradiation-based design of mechanically resistant microstructures tuned via multiscale phase-field modeling
Authors: Demange Gilles , Dépinoy Sylvain , Lunéville Laurence , Simeone David , Pontikis Vassilis 
Published: July 2018

AbstractWe present a multi-scale phase field modeling of stationary microstructures produced under 1 MeV krypton ion irradiation in a phase separating concentrated solid solution of silver and copper. We show that the mixture reaches ultimately a stationary micro-structural state made of phase domains with composition and size distribution mapped to the values of the incident flux of particles and of the temperature, variables that help defining a non equilibrium phase-diagram for the irradiated alloy. The modeling predicts the formation of diverse microstructures likely connected to spinodal hardening, thus opening the perspective of the on-purpose tuning of mechanically resistant microstructures and the preparation of metastable alloys with mechanical properties improved by comparison to counterparts obtained via classical thermo-mechanical treatments.
Rehabilitation mit einem demenzkranken Angehörigen: Ergebnisse einer längsschnittlichen Beobachtungstudie
Authors: Deck Ruth , Bussmann Marie-Luise , Simons Janine , Al-Hashimy Synan , Stark Michael 
Published: July 2018

Zusammenfassung
Hintergrund Pflegende Angehörige von Demenzerkrankten sind erheblichen Belastungen ausgesetzt. Ein multimodales Rehabilitationskonzept, welches die stationäre Behandlung von pflegerisch tätigen Angehörigen zusammen mit den Demenzkranken ermöglicht, wurde evaluiert.
Methodik Schriftliche Befragung von 121 pflegenden Angehörigen zu 3 Messzeitpunkten. Erfasst wurden Parameter der subjektiven Gesundheit sowie die Inanspruchnahme von pflegerischen und psychosozialen Unterstützungsangeboten im Verlauf.
Ergebnisse Es zeigen sich deutliche gesundheitliche Verbesserungen am Ende der Rehabilitation. Sechs Monate nach der Rehabilitation gehen die Effekte zurück, erreichen jedoch bei keinem der erfassten Merkmale den Ausgangswert vor der Reha.
Schlussfolgerungen Das multimodale Rehabilitationskonzept zeigt zumindest in Teilbereichen nachhaltige Effekte. Diese sowie die hohe Zufriedenheit der Teilnehmer sprechen dafür, dass sich das Rehabilitationskonzept bewährt und mit einem hohen gesundheitlichen Gewinn verbunden ist.
Dissociation of Platelet Activation and Spontaneous Myocardial Ischemia in Unstable Angina
Authors: Vejar Margarita , Fragasso Gabriele , Hackett David , Lipkin David P , Maseri Attilio , Born Gustav V R , Ciabattoni Giovanni , Patrono Carlo 
Published: July 2018

SummaryA dynamic thrombotic process, coronary spasm or both can be responsible for recurrent episodes of transient reduction of coronary blood flow in unstable angina. We have investigated the temporal relationship between episodic platelet activation, as detected by increased urinary excretion of 11-dehydro-TXB2, and spontaneous myocardial ischemia, assessed by continuous electrocardiographic monitoring and recording in 21 patients with unstable angina pectoris. In order to validate measurements of metabolite excretion as a reflection of intracoronary platelet activation, we have also performed repeated urine sampling from 8 patients undergoing PTCA and from 6 patients with peripheral vascular disease. The latter showed a 16% coefficient of variation in 3 consecutive 8-h urine samples. 11-dehydro-TXB2 increased significantly, by up to 15-fold, in the 2.5- to 5.0-h urine collection encompassing PTCA and decreased by > 50% during the following 2-h period. Patients with unstable angina were characterized by episodic increases (>2 SD of controls) in metabolite excretion, in successive 6-8 h specimens. Paired measurements of 11-dehydro-TXB2 and 2, 3-dinor-TXB2 in 15 urine samples did not reveal evidence of altered metabolic disposition of endogenously released TXB2. A total of 125 ECG ischemic episodes were recorded, of which 64% asymptomatic. We have compared these biochemical and ECG changes in patients randomized to i. v. low-dose aspirin or i.v. isosorbide dinitrate and oral diltiazem. Twenty-five of 56 (i.e. 45%) urine samples obtained in aspirin-free periods showed increased metabolite excretion as compared to 15 of 88 (i.e. 17%) samples collected during aspirin. Of the former, only 3 episodes of enhanced 11-dehydro-TXB2 excretion were associated with ST-segment changes, 7 with chest pain, and 15 with no ECG or clinical changes. Metabolite excretion was approximately 70% lower during aspirin administration than during coronary dilators. However, despite > 95% suppression of platelet cyclooxygenase activity, as monitored ex vivo, incomplete suppression of in vivo TXB2 biosynthesis was occasionally seen during low-dose aspirin therapy. We conclude that in unstable angina, episodic platelet activation is infrequently associated with spontaneous myocardial ischemia. Although the two events may represent functional expressions of the same coronary lesion, they are likely to be triggered by independent mechanisms through different mediators.
Development of MDL 28,050, a Small Stable Antithrombin Agent Based on a Functional Domain of the Leech Protein, Hirudin
Authors: Krstenansky John L , Broersma Robert J , Owen Thomas J , Payne Marguerite H , Yates Mark T , Mao Simon J T 
Published: July 2018

SummaryMDL 28,050 is a decapeptide antithrombin agent that inhibits a-thrombin-induced fibrin clot formation by binding to a non-catalytic site on α-thromhin. It is the result of chemical and structural optimization of a functional domain of the leech anticoagulant, hirudin. In contrast to the contention that the polyanionic nature of this C-terminal functional domain governs its interaction with α-thrombin, systematic study of this region has shown the importance of the lipophilic residues for providing the functionality necessary foi potent binding to a-thrombin. The development of MDL 28,050 and other effective antithrombin agents are outlined through the description of the structure-activity relationships (SAR) for these peptides. These peptides are effective in a variety of in vitro and in vivo models of thrombosis.
Observations on the Cell Biology of Tissue Factor in Endothelial Cells
Authors: Andoh Kiyoshi , Pettersen Kjell Sverre , Filion -Myklebust Christiane , Prydz Hans 
Published: July 2018

SummaryHuman umbilical vein endothelial cells (HUVEC) are inducible for tissue factor (TF) activity in culture. Based on experiments using ECGF (4–20 µg/ml) with heparin (90 µg/ml), we obtained the followingresults: 1) In confluent HUVEC cultures, ECGF had essentially no influence on the levels of inducible TF. 2) In growing HUVEC cultures, ECGF reduced the TF response shortly after seeding but full response was regained when cells were kept confluent for 2–3 days. 3) Although secondary cultures responded best to TF induction in the absence of ECGF, the response was essentially equal over at least 8 passages in the prcgcncc of ECGF 1) Of total cellular TF induced in HUVEC, about 25% was available on the surface, and less than 4% was released with theshed plasma membrane vesicles. The proportion of total TF a>ctivity available on the surface of mtact cells wasnot influenced by the presence of ECGF 5) T½, for the decay of TF activity induced was 8.3—9.5 h, whereas in HUVEC when protein synthesis was blocked afterIF induction a T½ of about 30 h was found.
Immunoelectrophoretic Analysis of Membrane Glycoproteins in Cultured Human Endothelial Cells
Authors: Børsum Tone 
Published: July 2018

SummaryHuman endothelial cells isolated from umbilical cordswere solubilized in Triton X-100 and examined by crossedimmunoelec-trophoresis using rabbit antiserum against endothelial cells. Endogenous labelling of the endothelialcell proteins with 14Cmannose followed by crossed immunoelectrophoresis and autoradiography revealed about 10 immunoprecipitates. Four of these endothelial cell glycoproteins were labelled by lactoperoxidase catalyzed iodination and thus were surface located. Three of the surface located glycoproteins showed reduced electrophoretic mobility after incubation of the endothelial cells with neuraminidase and were therefore sialoglycoproteins. Amphiphilicity of endothelial cell glycoproteins was studied by crossed hydrophobic interaction immunoelectrophoresis with phenyl-Sepharose in the intermediate gel. Amphiphilic proteins also show increasing electrophoretic migration velocity with decreasing concentration of Triton X-100 in the first dimension gels. Five of the endothelial cell glycoproteins were shown to be amphiphilic using these two techniques.Two monoclonal antibodies against the platelet glycoprotein complex Ilb-IIIa and glycoprotein IlIa, respectively, reacted with the same precipitate of endothelial cells. When a polyclonal antibody against the platelet glycoprotein complex Ilb-IIIa was incorporated into the intermediate gel the position of two endothelial cell precipitates were lowered. One of these was a sialoglycoprotein.
Monitoring Heparin Therapy: Relationships between the Activated Partial Thromboplastin Time and Heparin Assays Based on Ex-Vivo Heparin Samples
Authors: Bessekaar A M H P van den , Meeuwisse-Braun J , Bertina R M 
Published: July 2018

SummaryFive different APTT reagents, two amidolytic anti-ITa assays, one amidoiytic anti-Xa assay, and one coagulometric anti-Xa/ anti-IIa assay were used to assess the effect of heparin in patients treated for venous thromboembolic disease. Good correlations were observed between lug-transformed APYE> determined with the various reagents (correlation coefficients: 0.92-0.96).Nevertheless there were important differences in the slopes of the lines of relationship between the APTT reagents.Good correlations were observed between the anti-Xa and anti-IIa assay results (correlation coefficients: 0.92-0.97). However, the amidolytic anti-Xa activity was significantly higher (p <0.001) than the two amidolytic anti-IIa activities.
Less good correlations were observed between the log-transformed APTTs and the anti-Xa or anti-IIa activities (correlation coefficients: 0.64-0.78). The correlations were improved by transforming the APTT into APTT-ratio, i.e. the ratio of the patient’s APTT to the same patient’s APTT after removal of heparin from the plasma sample by means of ECTEOLA-cellulose treatment. The correlation coefficients of log (AFTT-ratio) with anti-Xa or anti-IIa ranged from 0.76 to 0.87.For both APTT and amidolytic heparin assay, the response to in vitro heparin was different from the response to ex vivo heparin.Therefore, equivalent therapeutic ranges should be assessed by using ex vivo samples rather than in vitro heparin. Because of the response differences between the APTT reagents, it is not adequate to define a therapeutic range for heparin therapy without specification of the reagent.
The Effect of Combined Factor XI Deficiency with von Willebrand Factor Abnormalities on Haemorrhagic Diathesis
Authors: Tavori Shulamith , Brenner Benjamin , Tatarsky llana 
Published: July 2018

SummaryTo account for the lack of correlation between the level of factor XI (FXI) in deficient patients and haemorrhagic manifestations, we correlated the prevalence of combined FXI and von Willebrand’s factor (vWF) deficiency in 212 FXI-deficient patients. Fifty-four patients had a combined FXI and vWF deficiency: 16 patients had severe and 38 patients had mild FXT deficiency. In a group of 28 patients with comparably mild FXI deficiency, 14 bleeders had significantly lower mean vWF, Ag, ristocetin cofactor and ristocetin induced platelet aggregation than 14 non-bleeders selected on the basis of comparable FXI levels. These findings suggest that the combination of FXI and vWF deficiency is common and may affect the bleeding tendency in mild FXI deficiency.
Platelet Modulation of Neutrophil Superoxide Anion Production
Authors: Moon Dudley G , Zee Hoyte van Der , Weston Lisa K , Gudewicz Paul W , Fenton II John W , Kaplan John E 
Published: July 2018

SummaryThe effect of platelets on polymorphonuclear leukocytes (PMN) O−
2 production was examined using autologous sheep and human cell systems. Coincubation of sheep platelets with sheep PMNs in the absence of thrombin resulted in a significant inhibition in basal PMN O−
2 production. The platelet-derived inhibitory activity was released into the medium and could be destroyed by adenosine deaminase suggesting that the inhibitor was adenosine. Addition of alpha-thrombin or platelet activating factor (PAF) enhanced PMN O−
2 production but only when platelets were present. The enhancement of O−
2 production in response to thrombin was dependent upon the thrombin concentration and the platelet-PMN ratio. With a platelet:PMN ratio of 30:1, addition of 10 nM thrombin to sheep cells resulted in a 5-fold increase in O−
2 production, whereas addition of 10 nM PAF caused a 2-fold increase in O−
2. Addition of thrombin or PAF to either PMNs or platelets by themselves did not initiate an increase in O−
2 generation. The response of human cells was similar except that both thrombin and PAF triggered a 2-fold increase in PMN O−
2 production in the presence of platelets. The platelet-derived enhancement activity was not released into the medium and was not blocked by WEB 2086, NDGA, ETYA, aspirin or adenosine deaminase. The enhancement effect appeared to be localized to the platelet membrane and we believe requires platelet-PMN contact.
The Effect of Different Anaesthetic Techniques on the Incidence of Thrombosis following Total Hip Replacement
Authors: Hoek Jaap A , Henny Ch Pieter , Knipscheer Haye C , ten Cate Hugo , Nurmohamed Michael T , ten Cate Jan W 
Published: July 2018

SummaryWe performed a retrospective analysis on the influence of three types of anaesthesia on the incidence of deep vein thrombosis (DVT) following total hip replacement (THR) in consecutive patients randomized to either the low molecular weight heparinoid Otg 10172 (97 patients), of placebo (99 patients). Ninety patients were operated under epidural anaesthesia, 77 patients under psoas compartment block with additional inhalation anaesthesia, and 29 patients under general anaesthesia. DVT assessment was performed by bilateral venography between days 8 and 12 postoperatively. The overall incidence of DVT in the 196 patients was 37% in the epidural anaesthasia group, 35% in the psoas compartment block group, and 36% in the general anaesthesia group. Although the incidence of DVT in patients randomuedto placebo was similar in the two anaesthesia groups (53%), there was an important reduction of the occurrence of proximal DVT by the heparinoid in the psoas compartment block group (from 20 to 0%), compared to the epidural anaesthesia group (from 27 to l8%) (p <0.0061). Significant y more minor wound hematomas occurred in the psoas compartment block group as compared to the epidural anaesthesia group (p <0.05). Synergrsm of thrombin generation inhibition by the heparinoid and inhibition of plateletlggregation at the damaged vessel wall, by high local concentrations of bupivacaine in the psoas compartment block technique, is proposed as a possible mechanism behind this observation.
Further Evidence that Glycoprotein IIb-IIIa Mediates Platelet Spreading on Subendothelium
Authors: Weiss Harvey J , Turitto Vincet T , Baumgartner Hans R 
Published: July 2018

SummaryIn order to explore further the mechanism by which glycoprotein GPIIb-IIIa promotes platelet vessel wall interaction, platelet adhesion to subendothelium was studied in an annular chamber in which subendothelium from rabbit aorta was exposed at a shear rate of 2,600 s−1 to blood from patients with thrombasthenia. Perfusions were conducted for each of 5 exposure times (1 ,2,3, 5 and 10 min), and the percent surface coverage of the vessel segment with platelets in the contact (C) and spread (S) stage was determined. Increased values of platelet contact (C) were obtained in thrombasthenia at all exposure times; this finding is consistent with a defect in platelet spreadirg, based on a previously described kinetic model of platelet attachment to subendothelium. According to this model of attachment, increased values of platelet contact (C) at a single exposure time may be indicative of either a defect in spreading (S) or initial contact (C), but multiple exposures will result in increased contact only for defects which are related to defectiye platelet spreading (s).The results obtained over a broad range of exposure times provide more conclusive evidence that GPIIb-IIIa mediates platelet spreading than those previously obtained at single exposure times.
Concanavalin A-induced Aggregation of Human Blood Platelets
Authors: Patscheke Heinrich , Brossmer Reinhard 
Published: July 2018

SummaryConcanavalin A (CON A) causes platelets to aggregate. A Ca++-independent effect of CON A could be separated from a main effect which depends on Ca++. The main effect probably is a consequence of the CON A-induced platelet release reaction and therefore is platelet-specific. The weak residual effect observed in the presence of Na2EDTA may be due to a similar mechanism as has been demonstrated for CON A-induced aggregations of several other normal and malignant transformed animal cells.Na2EDTA did not inhibit the carbohydrate-specific binding capacity of CON A. Therefore, Na2EDTA appears not to demineralize the CON A molecules under these experimental conditions.α-methyl-D-glucoside inhibits the Ca++-independent as well as the Ca++-dependent effect of CON A.Pretreatment by neuraminidase stimulated the platelet aggregation induced by CON A. It is possible that removal of terminal sialic acid residues makes additional receptors accessible for the binding of CON A.
The Effect of Halofenate or Halofenate Free Acid on Human, Rat and Guinea Pig Platelet Aggregation
Authors: Minsker D.H , Jordan P.T , Kling P , MacMillan A , Hucker H.B , Tocco D.J 
Published: July 2018

SummaryHalofenate free acid (HFA), the major metabolite of the hypolipemic agent halofenate, blocked the secondary phase of human platelet aggregation induced by ADP, epinephrine, or thrombin; higher concentrations of clohbrate free acid (CFA) were required to produce similar inhibitory effects on platelet aggregation. HFA and CFA inhibited collagen-induced aggregation of human, rat, or guinea pig platelets. Halofenate orally administered to rats caused inhibition of collagen-induced aggregation when plasma levels of HFA exceeded 300 μg/ml, a clinically achievable human plasma concentration. The platelet inhibitory effects of clofibrate administration were less than those observed with halofenate administration.
Comparison of Two Types of Endothelial Cells in Long Term Culture
Authors: Sedlak Bonnie Joy , Booyse F. M , Bell S , Rafelson M. E 
Published: July 2018

SummaryBovine aortic endothelial cells have been serially subcultured and maintained for ten months (24 passages) without any apparent loss of endothelial-like properties. There appear to be two endothelial cell types; one type is mononucleated, 40—60 μ in diameter, and the other (giant) cell type, multinucleate and 300-600 μ in diameter. Both cell types were identified as endothelial cells by established criteria; 1. ultrastructurally, by the presence of Weibel-Palade bodies, microfilaments and pinocytotic vesicles and 2. immunologically, by the presence of factor VIII antigen and thrombosthenin.
On the Value of the Activated Partial Thrombo-Plastin Time (APTT) in Monitoring Heparin Therapy
Authors: Teien Arne N. , Abildgaard Ulrich 
Published: July 2018

SummaryOf 1068 blood samples referred for evaluation of the haemostatic mechanism 135 contained heparin as judged by the polybrene titration method. Of the 57 samples with a heparin concentration in the defined therapeutic range, 65 per cent had APTT values in the “therapeutic range”. Of the samples from patients who had not received any anticoagulant, 213 (24 per cent) had prolonged APTT, and 48 of these samples had APTT values in the “therapeutic range” for heparin therapy. It is suggested that the APTT test is of limited value in monitoring heparin therapy.
Fibrinogen Response to Turpentine and Endotoxin in Busulfan-Treated Rabbits
Authors: Rapaport Samuel I. , Zivelin Ariella 
Published: July 2018

SummaryThe response of the plasma fibrinogen level to the subcutaneous injection of turpentine and to the intravenous injection of endotoxin was measured in normal rabbits and in rabbits made granulocytopenic and thrombocytopenic with busulfan. Plasma fibrinogen levels rose sharply in both normal and busulfan-treated rabbits but to a significantly lesser extent in the latter animals. A statistically significant relation was found between the number of residual granulocytes in busulfan-treated rabbits and the extent of the rise in fibrinogen level after turpentine. As discussed herein, these data are consistent with the hypothesis that material from granulocytes plays a pathophysiologic role in the stimulation of fibrinogen synthesis in inflammation and after tissue trauma.