bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of melatonin on regulation of apoptosis and steroidogenesis in cultured buffalo granulosa cells
Authors: Riaz Hasan , Yousuf Muhammad Rizwan , Liang Aixin , Hua Guo Hua , Yang Liguo 
Published: February 2019

AbstractThis study was aimed to address melatonin receptor expression, mRNA level of hypothalamus and hypophysis hormone receptors (GnRHR, FSHR, and LHR), steroidogenesis, cell cycle, apoptosis, and their regulatory factors after addition of melatonin for 24 hr in cultured buffalo granulosa cells (GCs). The results revealed that direct addition of different concentrations of melatonin (100 pM, 1 nM, and 100 nM) resulted in significant upregulation (p < 0.05) of mRNA level of melatonin receptor 1a (MT1) without affecting melatonin receptor 1b (MT2). Melatonin treatment significantly downregulated (p < 0.05) mRNA level of FSH and GnRH receptors, whereas 100 nM dose of melatonin significantly increased mRNA level of LH receptor. Treatment with 100 nM of melatonin significantly decreased the basal progesterone production with significant decrease (p < 0.05) in mRNA levels of StAR and p450ssc, and lower mRNA level of genes (Insig1, Lipe, and Scrab1) that affect cholesterol availability. Melatonin supplementation suppressed apoptosis (100 nM, p < 0.05) and enhanced G2/M phase (1 nM, 100 nM, p < 0.05) of cell cycle progression which was further corroborated by decrease in protein expression of caspase‐3, p21, and p27 and increase in bcl2. Our results demonstrate that melatonin regulates gonadotrophin receptors and ovarian steroidogenesis through MT1. Furthermore, the notion of its incorporation in apoptosis and proliferation of buffalo GCs extends its role in buffalo ovaries.
Examining the theory‐effectiveness hypothesis: A systematic review of systematic reviews
Authors: Dalgetty Rebecca , Miller Christopher B. , Dombrowski Stephan U. 
Published: February 2019

PurposeHealth interventions based on theory may be more effective than those that are not. This review of reviews synthesizes all published randomized controlled trial (RCT) meta‐analytic evidence from the last decade to examine whether theory‐based interventions were found to be associated with more effective adult health behaviour change interventions.MethodsSystematic reviews including meta‐analyses were identified by searching Medline, CINAHL, PsycINFO, and CDSR. A narrative synthesis was used to summarize and analyse the evidence. Only reviews including RCTs of health behaviour change interventions with adults aged 18+ published from 2007 to 2017 were included.ResultsOf 8,659 articles, nine systematic reviews met inclusion criteria. The majority of reviews (n = 8) suggested no increased effectiveness for theory‐based compared to non‐theory‐based interventions for effectiveness of outcomes relating to health behaviour. Less than half of the RCTs included in the reviews reported the use of theory (85/183). Two reviews suggested interventions based on control theory, motivational interviewing, or self‐determination theory were associated with greater effectiveness for physical activity and/or dietary interventions and outcomes. Methodological and reporting issues limit the conclusions.ConclusionsTheory‐based interventions as currently operationalized in systematic reviews were not found to be more effective than non‐theory‐based interventions. Methodological and reporting issues at study and review level may not reflect the true utility of theory use within health behaviour interventions. The promotion of theory use may benefit from using a multifaceted argument, rather than a narrow focus of increased effectiveness.
Statement of contribution

What is already known on this subject?

Theory use is regularly promoted by claiming that it will lead to more effective behaviour change interventions.

Theory use has been frequently linked to effectiveness within systematic reviews of behaviour change interventions.

The theory‐effectiveness hypothesis has not been systematically examined at the systematic review level.

What does this study add?

Theory use as operationalized by systematic review authors was not associated with increased effectiveness within systematic reviews examining randomized controlled trials of behaviour change interventions in adults.

Interventions based on control theory, motivational interviewing, or self‐determination theory were associated with greater effectiveness for physical activity and/or dietary interventions and outcomes.

Theory use should be promoted using a multifaceted argument, and assertions for increased effectiveness of theory‐based interventions should only be used in domains where specific evidence exists to support this claim.
Adaptive evolution of butterfly wing shape: from morphology to behaviour
Authors: Le Roy Camille , Debat Vincent , Llaurens Violaine 
Published: February 2019

AbstractButterflies display extreme variation in wing shape associated with tremendous ecological diversity. Disentangling the role of neutralversusadaptive processes in wing shape diversification remains a challenge for evolutionary biologists. Ascertaining how natural selection influences wing shape evolution requires both functional studies linking morphology to flight performance, and ecological investigations linking performance in the wild with fitness. However, direct links between morphological variation and fitness have rarely been established. The functional morphology of butterfly flight has been investigated but selective forces acting on flight behaviour and associated wing shape have received less attention. Here, we attempt to estimate the ecological relevance of morpho‐functional links established through biomechanical studies in order to understand the evolution of butterfly wing morphology. We survey the evidence for natural and sexual selection driving wing shape evolution in butterflies, and discuss how our functional knowledge may allow identification of the selective forces involved, at both the macro‐ and micro‐evolutionary scales. Our review shows that although correlations between wing shape variation and ecological factors have been established at the macro‐evolutionary level, the underlying selective pressures often remain unclear. We identify the need to investigate flight behaviour in relevant ecological contexts to detect variation in fitness‐related traits. Identifying the selective regime then should guide experimental studies towards the relevant estimates of flight performance. Habitat, predators and sex‐specific behaviours are likely to be major selective forces acting on wing shape evolution in butterflies. Some striking cases of morphological divergence driven by contrasting ecology involve both wing and body morphology, indicating that their interactions should be included in future studies investigating co‐evolution between morphology and flight behaviour.
The spatial genetic structure of the White‐banded Tanager (Aves, Passeriformes) in fragmented Neotropical savannas suggests two evolutionarily significant units
Authors: Lima‐Rezende Cássia Alves , de Souza Renata Oliveira , Caparroz Renato 
Published: February 2019

AbstractUnderstanding the genetic structure of a species is crucial for evolutionary biology research and species conservation. The objectives of this study were to investigate the genetic structure ofNeothraupis fasciatain Brazilian savannas and to assess genetic differentiation of its disjunct population in the Amazonian savannas of the state of Amapá. Population genetic structure was assessed in relation to isolation by distance and landscape variables connected with habitat heterogeneity. The influences of factors, such as habitat fragmentation and core–periphery distribution, on genetic diversity were also examined. Data were derived from a set of microsatellite loci of adult individuals from nine localities: eight distributed across the Cerrado and one in the disjunct Amazonian savanna of Amapá. Analysis revealed moderate genetic diversity and moderate population genetic structure, with at least two genetic clusters, one of which is represented exclusively by the disjunct Amapá population. The genetic structure found is not the result of significant influences by geographical distance, habitat heterogeneity, or the core–periphery effect, nor by intense biome fragmentation due to anthropic action. The disjunct Amapá population exhibited a moderate level of genetic differentiation compared to the Cerrado population, suggesting that both can be considered distinct evolutionarily significant units for conservation purposes.Abstract in Portuguese is available with online material.
T cells in severe childhood asthma
Authors: Paul Alberta G. A. , Muehling Lyndsey M. , Eccles Jacob D. , Woodfolk Judith A. 
Published: February 2019

SummarySevere asthma in children is a debilitating condition that accounts for a disproportionately large health and economic burden of asthma. Reasons for the lack of a response to standard anti‐inflammatory therapies remain enigmatic. Work in the last decade has shed new light on the heterogeneous nature of asthma, and the varied immunopathologies of severe disease, which are leading to new treatment approaches for the individual patient. However, most studies to date that explored the immune landscape of the inflamed lower airways have focused on adults. T cells are pivotal to the inception and persistence of inflammatory processes in the diseased lungs, despite a contemporary shift in focus to immune events at the epithelial barrier. This article outlines current knowledge on the types of T cells and related cell types that are implicated in severe asthma. The potential for environmental exposures and other inflammatory cues to condition the immune environment of the lung in early life to favour pathogenic T cells and steroid resistance is discussed. The contributions of T cells and their cytokines to inflammatory processes and treatment resistance are also considered, with an emphasis on new observations in children that argue against conventional type 1 and type 2 T cell paradigms. Finally, the ability for new technologies to revolutionize our understanding of T cells in severe childhood asthma, and to guide future treatment strategies that could mitigate this disease, is highlighted.
Liver cancer: WISP3 suppresses hepatocellular carcinoma progression by negative regulation of β‐catenin/TCF/LEF signalling
Authors: Gao Hong , Yin Fen‐Fen , Guan Dong‐Xian , Feng Yu‐Xiong , Zheng Qian‐Wen , Wang Xiang , Zhu Min , Zhang Xue‐Li , Cheng Shu‐Qun , Chen Tian‐Wei , Jiang Hao , Zhang Er‐Bin , Wang Jing‐Jing , Ni Qian‐Zhi , Yuan Yan‐Mei , Zhang Feng‐Kun , Ma Ning , Cao Hui‐Jun , Wang Yi‐Kang , Li Jing‐Jing , Xie Dong 
Published: February 2019

AbstractObjectivesWnt1‐inducible signalling pathway protein 3 (WISP3/CCN6) belongs to the CCN (CYR61/CTGF/NOV) family of proteins, dysregulation of this family contributed to the tumorigenicity of various tumours. In this study, we need to explore its role in hepatocellular carcinoma that remains largely elusive.Materials and MethodsThe expression of WISP3/CCN6 was analysed by qRT‐PCR and Western blotting. Effects of WISP3 on proliferation and metastasis of HCC cells were examined, respectively, by MTT assay and Boyden Chamber. Roles of WISP3 on HCC tumour growth and metastatic ability in vivo were detected in nude mice. Related mechanism study was confirmed by immunofluorescence and Western blotting.ResultsThe expression of WISP3 was significantly downregulated in HCC clinical samples and cell lines, and reversely correlated with the tumour size. Forced expression of WISP3 in HCC cells significantly suppressed cell growth and migration in vitro as well as tumour growth and metastatic seeding in vivo. In contrast, downregulation of WISP3 accelerated cell proliferation and migration, and promoted in vivo metastasis. Further study revealed that WISP3 inhibited the translocation of β‐catenin to the nucleus by activating glycogen synthase kinase‐3β (GSK3β). Moreover, constitutively active β‐catenin blocked the suppressive effects of WISP3 on HCC.ConclusionsOur study showed that WISP3 suppressed the progression of HCC by negative regulation of β‐catenin/TCF/LEF signalling, providing WISP3 as a potential therapeutic candidate for HCC.
Comparing web‐based video interventions to enhance university student willingness to donate organs: A randomized controlled trial
Authors: Thornton J. Daryl , Patrick Bridget , Sullivan Catherine , Albert Jeffrey M. , Wong Kristine A. , Allen Margaret D. , Kimble Linda , Mekesa Heather , Bowen Gordon , Sehgal Ashwini R. 
Published: February 2019

AbstractBackgroundThe efficacy of video interventions to increase organ donation willingness remains unclear.MethodsThree‐arm web‐based randomized controlled trial involving 2261 students at 3 northeastern Ohio universities. Intervention students watched a live‐action (n = 755) or animated (n = 753) donation video. Control students (n = 753) viewed wellness information from the Centers for Disease Control and Prevention (CDC). The primary outcome was proportion of students who visited their state electronic donor registry to consent. The secondary outcome was intervention quality. Logistic regression assessed the effects of interventions on visiting the state registry to provide donation consent while controlling for baseline variables.ResultsStudents in the live‐action video arm visited their state registry more frequently than students in the CDC arm (OR = 1.86, 95% CI = 1.20‐2.88). There was no difference between students in the animated video and CDC arms (OR = 1.10, 95% CI = 0.69‐1.76). The quality of the live‐action video was rated lower than the animated video and the CDC text (75% ± 18, 84% ± 16, 80% ± 16, respectively; P < 0.001).ConclusionStudents who watched the live‐action video were more willing to visit their electronic donor registry to register as organ donors, but rated it lower in satisfaction. Future work should identify the most potent components of organ donation interventions.
Urinary tract infections in solid organ transplant recipients: Guidelines from the American Society of Transplantation Infectious Diseases Community of Practice
Authors: Goldman Jason D. , Julian Kathleen 
Published: February 2019

AbstractThese updated guidelines from the Infectious Diseases Community of Practice of the American Society of Transplantation review the diagnosis, prevention, and management of urinary tract infections (UTI) in solid organ transplantation, focusing on kidney transplant (KT) recipients. KT recipients have unique risk factors for UTI, including indwelling stents and surgical manipulation of the genitourinary tract. KT recipients experience multi‐drug antibiotic‐resistant infections—UTI prevention and management strategies must consider risks of antimicrobial resistance. Non‐antimicrobial prevention strategies for UTI in KT recipients are reviewed. It is important to recognize that some renal transplant recipients with UTI may primarily present with fever, malaise, leukocytosis, or a non‐specific sepsis syndrome without symptoms localized to the urinary tract. However, asymptomatic bacteriuria (AB) must be distinguished from UTI because AB is not necessarily a disease state. Accumulating data indicate that there are no benefits of antibiotics for treatment of AB in KT recipients more than 2 months after post‐transplant. Further research is needed on management of AB in the early (<2 months) post‐transplant period, prophylaxis for UTI in this era of antibiotic resistance, recurrent UTI, non‐antimicrobial prevention of UTI, and uropathogens identified in donor urine and/or preservative fluid cultures.
Influence of impaired right ventricular contractile reserve on exercise capacity in patients with precapillary pulmonary hypertension: A study with exercise stress echocardiography
Authors: Guo Di‐Chen , Li Yi‐Dan , Yang Yuan‐Hua , Zhu Wei‐Wei , Sun Lan‐Lan , Jiang Wei , Ye Xiao‐Guang , Cai Qi‐Zhe , Lu Xiu‐Zhang 
Published: February 2019

ObjectivesRight ventricular (RV) contractile reserve reflects the ability of RV to accommodate the increased afterload and may play an essential role in the evaluation of precapillary pulmonary hypertension (PH). This study aimed to assess RV contractile reserve based on exercise stress echocardiography (ESE) and to determine the echocardiographic determinants of exercise capacity in patients with precapillary PH.MethodsA total of 31 patients with precapillary PH and 15 age‐ and sex‐matched healthy control subjects were prospectively recruited. All subjects underwent ESE to assess RV function at rest and under peak exercise. Changes in these parameters during exercise were calculated to quantify the RV contractile reserve. Patients with precapillary PH also underwent cardiopulmonary exercise test (CPET), and data pertaining to peak oxygen uptake (peak VO2) and minute ventilation/carbon dioxide production (VE/VCO2) were collected.ResultsRight ventricular contractile reserve including change in tricuspid annular plane systolic excursion (∆TAPSE), change in RV fractional area change (∆RVFAC), and change in Doppler‐derived tricuspid lateral annular peak systolic velocity (∆S′) was significantly depressed in precapillary PH patients compared with control subjects (P < 0.05). Parameters of RV function and RV contractile reserve were markedly associated with maximal exercise capacity (P < 0.05). ∆RVFAC was an independent predictor of peak VO2 (r2 = 0.601, P < 0.05).ConclusionsAssessment of RV contractile reserve facilitates identification of subclinical dysfunction and evaluation of clinical status and severity of precapillary PH. ESE as a noninvasive method may provide a comprehensive clinical assessment and facilitate therapeutic decision‐making for these patients.
Vascular and non‐vascular contributors to memory reduction during traumatic brain injury
Authors: Charkviani Mariam , Muradashvili Nino , Lominadze David 
Published: February 2019

AbstractTraumatic brain injury (TBI) is an increasing health problem. It is a complex, progressive disease that consists of many factors affecting memory. Studies have shown that increased blood‐brain barrier (BBB) permeability initiates pathological changes in neuro‐vascular network but the role of cerebrovascular dysfunction and its mediated mechanisms associated with memory reduction during TBI are still not well understood. Changes in BBB, inflammation, extravasation of blood plasma components, activation of neuroglia lead to neurodegeneration. Extravasated proteins such as amyloid‐beta, fibrinogen, and cellular prion protein may form degradation resistant complexes that can lead to neuronal dysfunction and degeneration. They also have the ability to activate astrocytes, and thus, can be involved in memory impairment. Understanding the triggering mechanisms and the places they originate in vasculature or in extravascular tissue may help to identify potential therapeutic targets to ameliorate memory reduction during TBI. The goal of this review is to discuss conceptual mechanisms that lead to short‐term memory reduction during non‐severe TBI considering distinction between vascular and non‐vascular effects on neurons. Some aspects of these mechanisms need to be confirmed further. Therefore, we hope that the discussion presented bellow may lead to experiments that may clarify the triggering mechanisms of memory reduction after head trauma.
Clinical, neuroimaging and prognostic study of 127 cases with infarction of the corpus callosum
Authors: Sun X. , Li J. , Fan C. , Zhang H. , Si Y. , Fang X. , Guo Y. , Zhang J. H. , Wu T. , Ding S. , Bi X. 
Published: February 2019

Background and purposeThe aim of this study was to retrospectively investigate clinical and neuroimaging characteristics in the largest sample size of patients with corpus callosum infarction to date and then to follow up these patients for 1 year to clarify the prognosis of this rare stroke entity.MethodsA total of 127 patients with acute callosal infarction out of 5584 acute ischaemic stroke patients were included in this study. The recruited patients were divided into a pure callosal infarction group and a complex callosal infarction group (coupled with other infarct locations simultaneously), and clinical and neuroimaging features were analyzed. Some of the patients were followed up for 1 year to evaluate recurrence rate and mortality.ResultsThe incidence of acute callosal infarction was 2.3%. Most patients presented with advanced neurological dysfunction with or without mild to moderate motor or sensory disorders on admission. The negative rate of computed tomography scan was still 76.4% even at >24 h after onset. Large‐artery atherosclerosis was the most common etiological type. Compared with complex callosal infarction, the pure callosal infarction group had more mental disorders (P = 0.030). Compared with common basal ganglia infarction, the pure callosal infarction group had better short‐term recovery (P = 0.016) but higher 1‐year mortality (P = 0.037). Age and mental disorders were independent risk factors for death in callosal infarction.ConclusionsCallosal infarction is a white matter stroke that occurs with low incidence. Elderly patients with vascular risk factors showed sudden mental or cognitive disorders and callosal infarction could not be excluded. More attention should be paid to the early diagnosis and secondary prevention of callosal infarction because of its poor long‐term outcome.
Defence tactics cycle with diel microhabitat choice and body temperature in the desert locust,Schistocerca gregaria
Authors: Maeno Koutaro Ould , Ould Ely Sidi , Ould Mohamed Sid’Ahmed , Jaavar Mohamed El Hacen , Nakamura Satoshi , Ould Babah Ebbe Mohamed Abdallahi 
Published: February 2019

AbstractWe examined environmental factors influencing plasticity in antipredator defences of adult gregarious desert locusts,Schistocerca gregaria, including daily cycles in temperature, light, microhabitat occupied and predator threat. In the Sahara Desert in Mauritania, West Africa, daily temperature fluctuated widely from below locusts’ cold thermal limits for jump‐ and flight‐defence, to above their preferred body temperature. Locusts changed microhabitats throughout the 24‐hr period in synchrony with the daily thermo‐photocycle. They roosted in tall trees and large bushes at night, moved to the ground in the morning, shaded under or in small bushes and annuals at midday, moved back to the ground in the afternoon and then returned to night roosting sites around dusk. Locust antipredator defences varied throughout the 24‐hr period, and these changes were correlated with temperature, photocycle and habitat. Flight escape was associated with daytime, high temperatures and the ground habitat. Dropping escape (= releasing hold of vegetation and dropping to the ground or into vegetation) was associated with cool temperatures and low‐to‐medium sized bushes. Stationary behaviour was associated with the tree microhabitat and height off the ground. Roosting (a primary defence) was associated with cool temperatures at night and early morning, tree habitats and nocturnal ground‐foraging times of endothermic mammals. In summary, we propose that temperature is the key factor in determining both changing microhabitat choice and changing antipredator defence, due to thermal constraints on locust muscle for this ectothermic insect. The thermocycle also influences temporal predator loads, which influence the evolution of locust diel defence strategies. These various environmental factors not only influence one another, they also interact to influence antipredator defence expression in locusts. Overall, our study suggests that plasticity in locust antipredator defences is a complicated matter mediated by the interactions of multiple environmental factors and physiological and ecological constraints and trade‐offs.