bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enrichment of hematopoietic stem/progenitor cells in the zebrafish kidney
Authors: Kobayashi Isao , Kondo Mao , Yamamori Shiori , Kobayashi-Sun Jingjing , Taniguchi Makoto , Kanemaru Kaori , Katakura Fumihiko , Traver David 
Published: October 2019

AbstractHematopoietic stem cells (HSCs) maintain the entire blood system throughout life and are utilized in therapeutic approaches for blood diseases. Prospective isolation of highly purified HSCs is crucial to understand the molecular mechanisms underlying regulation of HSCs. The zebrafish is an elegant genetic model for the study of hematopoiesis due to its many unique advantages. It has not yet been possible, however, to purify HSCs in adult zebrafish due to a lack of specific HSC markers. Here we show the enrichment of zebrafish HSCs by a combination of two HSC-related transgenes, gata2a:GFP and runx1:mCherry. The double-positive fraction of gata2a:GFP and runx1:mCherry (gata2a+runx1+) was detected at approximately 0.16% in the kidney, the main hematopoietic organ in teleosts. Transcriptome analysis revealed that gata2a+runx1+ cells showed typical molecular signatures of HSCs, including upregulation of gata2b, gfi1aa, runx1t1, pbx1b, and meis1b. Transplantation assays demonstrated that long-term repopulating HSCs were highly enriched within the gata2a+runx1+ fraction. In contrast, colony-forming assays showed that gata2a−runx1+ cells abundantly contain erythroid- and/or myeloid-primed progenitors. Thus, our purification method of HSCs in the zebrafish kidney is useful to identify molecular cues needed to regulate self-renewal and differentiation of HSCs.
p-wave superconductivity in iron-based superconductors
Authors: Talantsev E. F. , Iida K. , Ohmura T. , Matsumoto T. , Crump W. P. , Strickland N. M. , Wimbush S. C. , Ikuta H. 
Published: October 2019

AbstractThe possibility of p-wave pairing in superconductors has been proposed more than five decades ago, but has not yet been convincingly demonstrated. One difficulty is that some p-wave states are thermodynamically indistinguishable from s-wave, while others are very similar to d-wave states. Here we studied the self-field critical current of NdFeAs(O,F) thin films in order to extract absolute values of the London penetration depth, the superconducting energy gap, and the relative jump in specific heat at the superconducting transition temperature, and find that all the deduced physical parameters strongly indicate that NdFeAs(O,F) is a bulk p-wave superconductor. Further investigation revealed that single atomic layer FeSe also shows p-wave pairing. In an attempt to generalize these findings, we re-examined the whole inventory of superfluid density measurements in iron-based superconductors and show quite generally that single-band weak-coupling p-wave superconductivity is exhibited in iron-based superconductors.
Electroosmotic flow driven microfluidic device for bacteria isolation using magnetic microbeads
Authors: Miller Samuel , Weiss Alison A. , Heineman William R. , Banerjee Rupak K. 
Published: October 2019

AbstractThe presence of bacterial pathogens in water can lead to severe complications such as infection and food poisoning. This research proposes a point-of-care electroosmotic flow driven microfluidic device for rapid isolation and detection of E. coli in buffered solution (phosphate buffered saline solution). Fluorescent E. coli bound to magnetic microbeads were driven through the microfluidic device using both constant forward flow and periodic flow switching at concentrations ranging from 2 × 105 to 4 × 107 bacteria/mL. A calibration curve of fluorescent intensity as a function of bacteria concentration was created using both constant and switching flow, showing an increase in captured fluorescent pixel count as concentration increases. In addition, the use of the flow switching resulted in a significant increase in the capture efficiency of E. coli, with capture efficiencies up to 83% ± 8% as compared to the constant flow capture efficiencies (up to 39% ± 11%), with a sample size of 3 µL. These results demonstrate the improved performance associated with the use of the electroosmotic flow switching system in a point-of-care bacterial detection assay.
New method to calculate the dynamic factor–flow velocity in Geomorphologic instantaneous unit hydrograph
Authors: Chen Yingbing , Shi Peng , Ji Xiaomin , Qu Simin , Zhao Lanlan , Dong Fengcheng 
Published: October 2019

AbstractThe determination of characteristic flow velocity is a hydrodynamic problem needs to be solved in the application of geomorphologic instantaneous unit hydrograph (GIUH) for runoff simulation in areas with no or limited data. In this study, 120 watersheds are collected to construct a regression model; 85 of these basins are used for regression analysis, and the 35 remaining basins are utilized to verify the feasibility of the constructed model. Random forest algorithm is applied to screen out important geomorphologic factors from the 16 extracted factors that may affect flow velocity. Multivariate regression is used to establish the numerical relationship between velocity and the selected factors. Sensitivity analysis of each adopted factor in the constructed model is conducted using the LH-OAT method. The rationality and feasibility of the regression model are validated by comparing the flow velocity calculation with a previous approach, which is also calculated based on geomorphological parameters. Subsequently, the runoff simulation based on the GIUH model is evaluated using the proposed technique. Results demonstrate that the proposed formula possesses high fitting accuracy and can be easily used to calculate flow velocity and generate GIUH.
Targeting CDC7 sensitizes resistance melanoma cells to BRAFV600E-specific inhibitor by blocking the CDC7/MCM2-7 pathway
Authors: Gad Shaimaa A. , Ali Hamdy E. A. , Gaballa Rofaida , Abdelsalam Rania M. , Zerfaoui Mourad , Ali Hamed I. , Salama Salwa H. , Kenawy Sanaa A. , Kandil Emad , Abd Elmageed Zakaria Y. 
Published: October 2019

AbstractAlthough the utilization of selective BRAFV600E inhibitors is associated with improved overall survival in patients with metastatic melanoma, a growing challenge of drug resistance has emerged. CDC7 has been shown to be overexpressed and associated with poor prognosis in various cancers including melanoma. Thus, we aimed to elucidate the biological role of CDC7 in promoting Vemurafenib resistance and the anticipated benefits of dual targeting of BRAFV600E and CDC7 in melanoma cells. We performed exosomes-associated microRNA profiling and functional assays to determine the role of CDC7 in drug resistance using Vemurafenib-sensitive and resistant melanoma cells. Our results demonstrated that Vemurafenib-resistant cells exhibited a persistent expression of CDC7 in addition to prolonged activity of MCM2 compared to drug-sensitive cells. Reconstitution of miR-3613-3p in resistant cells downregulated CDC7 expression and reduced the number of colonies. Treatment of cells with low concentrations of CDC7 inhibitor TAK-931 sensitized resistant cells to Vemurafenib and reduced the number of cell colonies. Taken together, CDC7 overexpression and downregulation of miR-3613-3p were associated with Vemurafenib resistance in BRAFV600E- bearing melanoma cells. Dual targeting of CDC7 and BRAFV600E reduced the development of resistance against Vemurafenib. Further studies are warranted to investigate the clinical effect of targeting CDC7 in metastatic melanoma.
Reconstruction of apo A2A receptor activation pathways reveal ligand-competent intermediates and state-dependent cholesterol hotspots
Authors: Lovera Silvia , Cuzzolin Alberto , Kelm Sebastian , De Fabritiis Gianni , Sands Zara A. 
Published: October 2019

AbstractG-protein coupled receptors (GPCRs) play a pivotal role in transmitting signals at the cellular level. Structural insights can be exploited to support GPCR structure-based drug discovery endeavours. Despite advances in GPCR crystallography, active state structures are scarce. Molecular dynamics (MD) simulations have been used to explore the conformational landscape of GPCRs. Efforts have been made to retrieve active state conformations starting from inactive structures, however to date this has not been possible without using an energy bias. Here, we reconstruct the activation pathways of the apo adenosine receptor (A2A), starting from an inactive conformation, by applying adaptive sampling MD combined with a goal-oriented scoring function. The reconstructed pathways reconcile well with experiments and help deepen our understanding of A2A regulatory mechanisms. Exploration of the apo conformational landscape of A2A reveals the existence of ligand-competent states, active intermediates and state-dependent cholesterol hotspots of relevance for drug discovery. To the best of our knowledge this is the first time an activation process has been elucidated for a GPCR starting from an inactive structure only, using a non-biased MD approach, opening avenues for the study of ligand binding to elusive yet pharmacologically relevant GPCR states.
A model and simulation of the emotional contagion of netizens in the process of rumor refutation
Authors: Zeng Runxi , Zhu Di 
Published: October 2019

AbstractThe emotional contagion of netizens is an important factor that accelerates the spread of rumors, and it is also key to the effectiveness of rumor refutation. Based on the existing emotional model, we improved the method for calculating the emotional value and the transformation rules to simulate how the infection transforms individual emotion to group emotion during rumor refutation. The results show that the cycle and trend of netizen emotional change vary by period, but the final distribution structure presents a relatively stable state. The factors that affect the emotional changes of netizens are mainly objective and subjective aspects, both of which can promote the evolution of emotional contagion. The objective aspect depends on the speed and credibility of the rumor, and the subjective aspect depends on the degree of intimacy between netizens. After rumor refutation, emotions generally change from negative emotions to positive or immune emotions.
Facile Synthesis of Mesoporous α-Fe2O3@g-C3N4-NCs for Efficient Bifunctional Electro-catalytic Activity (OER/ORR)
Authors: Alduhaish Osamah , Ubaidullah Mohd , Al-Enizi Abdullah M. , Alhokbany Norah , Alshehri Saad M. , Ahmed Jahangeer 
Published: October 2019

AbstractMesoporous α-iron oxide@graphitized-carbon nitride nanocomposites (α-Fe2O3@g-C3N4-NCs) were synthesized using urea-formaldehyde (UF) resins at 400 °C/2 h. The mesoporous nature of the prepared nanocomposites was observed from electron microscopy and surface area measurements. The electrochemical measurements show the bifunctional nature of mesoporous α-Fe2O3@g-C3N4-NCs in electrolysis of water for oxygen evolution and oxygen reduction reactions (OER/ORR) using 0.5 M KOH. Higher current density of mesoporous α-Fe2O3@g-C3N4-NCs reveals the enhanced electrochemical performance compared to pure Fe2O3 nanoparticles (NPs). The onset potential, over-potential and Tafel slopes of mesoporous α-Fe2O3@g-C3N4-NCs were found lower than that of pure α-Fe2O3-NPs. Rotating disc electrode experiments followed by the K-L equation were used to investigate 4e− redox system. Therefore, the mesoporous α-Fe2O3@g-C3N4-NCs bifunctional electro-catalysts can be considered as potential future low-cost alternatives for Pt/C catalysts, which are currently used in fuel cells.
Widespread brain reorganization perturbs visuomotor coordination in early glaucoma
Authors: Trivedi Vivek , Bang Ji Won , Parra Carlos , Colbert Max K. , O’Connell Caitlin , Arshad Ahmel , Faiq Muneeb A. , Conner Ian P. , Redfern Mark S. , Wollstein Gadi , Schuman Joel S. , Cham Rakie , Chan Kevin C. 
Published: October 2019

AbstractGlaucoma is the world’s leading cause of irreversible blindness, and falls are a major public health concern in glaucoma patients. Although recent evidence suggests the involvements of the brain toward advanced glaucoma stages, the early brain changes and their clinical and behavioral consequences remain poorly described. This study aims to determine how glaucoma may impair the brain structurally and functionally within and beyond the visual pathway in the early stages, and whether these changes can explain visuomotor impairments in glaucoma. Using multi-parametric magnetic resonance imaging, glaucoma patients presented compromised white matter integrity along the central visual pathway and around the supramarginal gyrus, as well as reduced functional connectivity between the supramarginal gyrus and the visual occipital and superior sensorimotor areas when compared to healthy controls. Furthermore, decreased functional connectivity between the supramarginal gyrus and the visual brain network may negatively impact postural control measured with dynamic posturography in glaucoma patients. Taken together, this study demonstrates that widespread structural and functional brain reorganization is taking place in areas associated with visuomotor coordination in early glaucoma. These results implicate an important central mechanism by which glaucoma patients may be susceptible to visual impairments and increased risk of falls.
A Fast Parameter Identification Framework for Personalized Pharmacokinetics
Authors: Yang Chenxi , Tavassolian Negar , Haddad Wassim M. , Bailey James M. , Gholami Behnood 
Published: October 2019

AbstractThis paper introduces a novel framework for fast parameter identification of personalized pharmacokinetic problems. Given one sample observation of a new subject, the framework predicts the parameters of the subject based on prior knowledge from a pharmacokinetic database. The feasibility of this framework was demonstrated by developing a new algorithm based on the Cluster Newton method, namely the constrained Cluster Newton method, where the initial points of the parameters are constrained by the database. The algorithm was tested with the compartmental model of propofol on a database of 59 subjects. The average overall absolute percentage error based on constrained Cluster Newton method is 12.10% with the threshold approach, and 13.42% with the nearest-neighbor approach. The average computation time of one estimation is 13.10 seconds. Using parallel computing, the average computation time is reduced to 1.54 seconds, achieved with 12 parallel workers. The results suggest that the proposed framework can effectively improve the prediction accuracy of the pharmacokinetic parameters with limited observations in comparison to the conventional methods. Computation cost analyses indicate that the proposed framework can take advantage of parallel computing and provide solutions within practical response times, leading to fast and accurate parameter identification of pharmacokinetic problems.
Quantum control of excitons for reversible heat transfer
Authors: Murphy Conor N. , Eastham Paul R. 
Published: October 2019

AbstractLasers, photovoltaics, and thermoelectrically-pumped light emitting diodes are thermodynamic machines which use excitons (electron-hole pairs) as the working medium. The heat transfers in such devices are highly irreversible, leading to low efficiencies. Here we predict that reversible heat transfers between a quantum-dot exciton and its phonon environment can be induced by laser pulses. We calculate the heat transfer when a quantum-dot exciton is driven by a chirped laser pulse. The reversibility of this heat transfer is quantified by the efficiency of a heat engine in which it forms the hot stroke, which we predict to reach 95% of the Carnot limit. This performance is achieved by using the time-dependent laser-dressing of the exciton to control the heat current and exciton temperature. We conclude that reversible heat transfers can be achieved in excitonic thermal machines, allowing substantial improvements in their efficiency.
Optimising nudges and boosts for financial decisions under uncertainty
Authors: Franklin Matija , Folke Tomas , Ruggeri Kai 
Published: October 2019

AbstractBehavioural interventions that directly influence decision-making are increasingly popular policy tools. Two prominent interventions used are nudges, which promote an optimal choice without restricting options, and boosts, which promote individual capabilities to make more informed choices. Direct comparison is a critical step toward understanding the populations and contexts where they may be most efficient, or potentially complementary toward improving their effectiveness. Two trials in the US and Serbia (N = 1423) tested a series of choices under uncertainty using both nudge and boost interventions. In a replication setting, hypothetical and consequential decisions are used. Findings indicate that disclosure nudges and boosts, unlike social nudges, promote more advantageous financial decisions. Furthermore, the effects of disclosure nudges and boosts generally differ depending on loss and gain framing—boosts promoted more advantageous decisions under gain frames while disclosure nudges did so under loss frames. Finally, boosts were typically more effective for those who initially made suboptimal choices and sociodemographic factors did not mediate the effectiveness of the interventions. These insights provide clarity to highly nuanced, complex patterns across population behaviours in the context of financial choice under uncertainty and considerable implications for the design of interventions for policies that impact population behaviours.
Simple symmetrical circuits and a comment on the importance of topology
Authors: Wörner C H 
Published: October 2019

Abstract
The equivalent resistance calculation for two circuits in cubic arrangements is solved. Emphasis is placed on the plastic (topological) properties of these circuits. In contrast, the opposite topological behaviour of an analogous arrangement is observed in the calculus of a magnetic field. It is also noted that the solved examples may be used as a teaser/comic pedagogical approach to physics instruction.
Distinguishing Majorana zero modes from impurity states through time-resolved transport
Authors: Tuovinen Riku , Perfetto Enrico , Leeuwen Robert van , Stefanucci Gianluca , Sentef Michael A 
Published: October 2019

Abstract
We study time-resolved charge transport in a superconducting nanowire using time-dependent Landauer–Büttiker theory. We find that the steady-state Majorana zero-bias conductance peak emerges transiently accompanied by characteristic oscillations after a bias-voltage quench. These oscillations are suppressed for trivial impurity states (IS) that otherwise show a similar steady-state signal as the Majorana zero mode (MZM). In addition, we find that Andreev bound states or quasi-Majorana states (QMS) in the topologically trivial bulk phase can give rise to a zero-bias conductance peak, also retaining the transient properties of the MZM. Our results imply that (1) time-resolved transport may be used as a probe to distinguish between the topological MZM and trivial IS; and (2) the QMS mimic the transient signatures of the topological MZMs.
A succinct review on fuel cells
Authors: Suraparaju Subbarama Kousik , Natarajan Sendhil Kumar , Karthikeyan P 
Published: October 2019

AbstractThe role of renewable energy is becoming significant in the current scenario due to increasing power demand, instability of fuel prices and economic-environmental glitches. Fuel cell stood as one of the emerging and leading contributors in the realm of renewable energy because of its higher efficacy, cleanliness and economic supply of demanded energy to the consumers. This article gives a brief knowledge on the different types of fuel cell technologies along with their working principles, pros, cons and application of fuel cells in various sectors. It also discusses the techno-economic issues of this current technology. Also briefed about the future recommendations to wide spread this technology for better economic and environmental solutions.
Minimization of parallax error in positron emission tomography using depth of interaction capable detectors: methods and apparatus
Authors: Mohammadi I , Castro I F C , Correia P M M , Silva A L M , Veloso J F C A 
Published: October 2019

Abstract
In this paper, the authors review the field of parallax error (PE) minimization in positron emission tomography (PET) imaging systems by using depth of interaction (DOI) capable concepts. The review includes apparatus as well as an overview of various methods described in the literature. It also discusses potential advantages gained via these approaches, as discussed with reference to various metrics and tasks, particularly in the improvement of spatial resolution (SR) performance. Furthermore, the authors emphasize limitations encountered in the context of DOI decoding, which can be a considerable pitfall depending on the task of interest.
Heterogeneous Exposure Associations in Observational Cohort Studies: The Example of Blood Pressure in Older Adults
Authors: Odden Michelle C , Rawlings Andreea M , Khodadadi Abtin , Fern Xiaoli , Shlipak Michael G , Bibbins-Domingo Kirsten , Covinsky Kenneth , Kanaya Alka M , Lee Anne , Haan Mary N , Newman Anne B , Psaty Bruce M , Peralta Carmen A 
Published: October 2019

Abstract
Heterogeneous exposure associations (HEAs) can be defined as differences in the association of an exposure with an outcome among subgroups that differ by a set of characteristics. In this article, we intend to foster discussion of HEAs in the epidemiologic literature and present a variant of the random forest algorithm that can be used to identify HEAs. We demonstrate the use of this algorithm in the setting of the association between systolic blood pressure and death in older adults. The training set included pooled data from the baseline examination of the Cardiovascular Health Study (1989–1993), the Health, Aging, and Body Composition Study (1997–1998), and the Sacramento Area Latino Study on Aging (1998–1999). The test set included data from the National Health and Nutrition Examination Survey (1999–2002). The hazard ratios ranged from 1.25 (95% confidence interval: 1.13, 1.37) per 10-mm Hg increase in systolic blood pressure among men aged ≤67 years with diastolic blood pressure greater than 80 mm Hg to 1.00 (95% confidence interval: 0.96, 1.03) among women with creatinine concentration ≤0.7 mg/dL and a history of hypertension. HEAs have the potential to improve our understanding of disease mechanisms in diverse populations and guide the design of randomized controlled trials to control exposures in heterogeneous populations.