bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Penetrating Facial Wound With Burn Injury
Authors: Duan Ran , Shi Jun , Tremp Mathias , Oranges Carlo M. , Gao Bowen , Xie Feng , Li Qingfeng 
Published: August 2018

Abstract
A patient presented with a complex penetrating facial wound by high temperature steel. The hot steel penetrated right temple, ethmoid bone, and maxillary sinus, and then exited from his left cheek. He kept his right eye but lost his sight. For functional and esthetic considerations, treatments were provided in a staged procedure. First, the debridement was performed under the endoscope. The dead bone was removed, broken teeth were extracted, and necrotic tissue was cleaned. The exposed wounds were cleaned, and dressing was changed daily until the exudation was widely reduced. Then, a 50-mL expander was placed in the left cheek, and an 80-mL expander was put in the scalp just before the defect in the temple area. Five months later, expander inflation was accomplished. Expanders were taken out and expanded flaps were transposed to cover the defect. At the same time, an anterolateral thigh flap was harvested to repair the inner lining of the cheek and the gingiva. Thereafter, several operations were performed to revise the wound scar and the remaining deformity. Both defects in the temple and left cheek were restored with a satisfactory functional and aesthetic outcome. The temporal area was repaired with haired expanded scalp, whereas the face was repaired with an expanded facial flap with similar texture and color. The oral commissure regained balance and integrity. The defect of the gingiva was repaired. A severe penetrating wound in the cranium and face can be nicely repaired using tissue expander and microvascular reconstruction.
Acceleration of Fracture Healing in Experimental Model: Platelet-Rich Fibrin or Hyaluronic Acid?
Authors: Akyildiz Servet , Soluk-Tekkesin Merva , Keskin-Yalcin Basak , Unsal Gurkan , Ozel Yildiz Sevda , Ozcan Ilknur , Cakarer Sirmahan 
Published: August 2018

Abstract
In this study, we compared the bone-healing effects of the local application of platelet-rich fibrin (PRF) and hyaluronic acid (HA) to bilateral tibial fractures in rats. Twenty-three adult male Sprague-Dawley rats were used. Twenty-two animals were randomly allocated to a control group (n = 6) and 2 study groups: PRF (n = 8) and HA (n = 8). The 23rd rat was used as a donor to obtain PRF. Each group was divided into 2 subgroups for histomorphometric and radiologic assessments at 2 and 6 weeks. Foreign body reaction, necrosis, inflammation, new bone formation, and fibrosis were investigated as bone healing parameters in terms of histopathologic analysis. The difference between the groups for these parameters was evaluated. The radiologic evaluation was performed by comparing the 3-dimensional reconstruction images of the fracture sites between the study and control groups. Histomorphometric evaluation showed that at 2 weeks postoperatively, the control group showed lesser bone formation (26.1 ± 6.6%) when compared to the study (HA: 54.7 ± 9.7%; PRF: 75.3 ± 19.2%) groups and PRF group showed highest total ossification. At 6 weeks postoperatively the PRF group showed lesser total ossification (50.7 ± 28.2%) when compared to control (76.3 ± 21.7%) and HA group. The HA (88.8 ± 13.3%) showed highest total ossification. In the control group, fibrosis was more prominent at week 6, whereas in the HA and PRF groups the amount of ossification increased. In contrast to histopathologic healing, radiologic bone healing did not differ significantly among the study and control groups 2 weeks after surgery, whereas at 6 weeks, the results of radiologic bone formation were in accordance with those of histopathologic bone healing.
Transnasal Endoscopic Removal of a Knife Causing Penetrating Brain Injury in a Child
Authors: Yücel Abitter , Yücel Hilal , Güllüev Mustafa , Alataş Necat 
Published: August 2018

Abstract
Transnasal penetrating brain injuries are rare and a medical emergency, which needs to be treated promptly. A 4-year-old male patient was brought to our emergency room with a knife sticking out of his nose. The patient was immediately taken to the operating room and the knife was removed under general anesthesia. No cerebrospinal fluid leakage or any bleeding was seen and so the operation was terminated. The authors herein report a penetrating brain trauma through to the cella turcica with a knife that improved without any sequelae at a child.
Recipient Age and Mortality After Liver Transplantation: A Population-based Cohort Study
Authors: Gil Eunmi , Kim Jong Man , Jeon Kyeongman , Park Hyejeong , Kang Danbee , Cho Juhee , Suh Gee Young , Park Jinkyeong 
Published: August 2018

Background
The feasibility of liver transplantation (LT) in elderly recipients remains a topic of debate.

Methods
This cohort study evaluated the impact of recipient's age on LT outcome between January 2007 and May 2016 covered by the Korean National Health Insurance system (n = 9415). Multilevel regression models were used to determine the impact of recipient's age on in-hospital and long-term mortality after LT.

Results
All patients had a first LT, with 2473 transplanted with liver from deceased donors (DD) and 6942 from living donors. The mean age was 52.2 ± 9.0 years. Most LT were performed on patients in their 50s (n = 4290, 45.6%) and 0.9% (n = 84) of the LT was performed on patients older 70 years. The overall in-hospital mortality was 6.3%, and the 3-year mortality was 11.3%. The in-hospital mortality included, 13.5% associated with DDLT and 3.7% involved living donor LT. When compared with that for patients aged 51 to 55 years, the risk of death among recipients older than 70 years was about fourfold higher after adjusting for baseline liver disease (odds ratio, 4.1; 95% confidence interval, 2.21-7.58), and was nearly threefold higher after adjusting for baseline liver disease and perioperative complications (odds ratio, 2.92; 95% confidence interval, 1.37-6.24). Also, the cost of LT increased significantly with age.

Conclusions
The data show that age remains an important risk factor for LT, suggesting that LT should be considered with caution in elderly recipients.
Indication of spatially random infection of chlamydia-like organisms in Bufo bufo tadpoles from ponds located in the Geneva metropolitan area
Authors: Vajana Elia , Widmer Ivo , Rochat Estelle , Duruz Solange , Selmoni Oliver , Vuilleumier Séverine , Aeby Sébastien , Greub Gilbert , Joost Stéphane 
Published: August 2018

AbstractOccurrence of bacteria belonging to the order Chlamydiales was investigated for the first time in common toad (Bufo bufo) tadpole populations collected from 41 ponds in the Geneva metropolitan area, Switzerland. A Chlamydiales-specific Real-Time PCR was used to detect and amplify the Chlamydiales 16S rRNA-encoding gene from the tails of 375 tadpoles. We found the studied amphibian populations to be infected by “Chlamydia-like organisms” (CLOs) attributable to the genera Similichlamydia, Neochlamydia, Protochlamydia and Parachlamydia (belonging to the family Parachlamydiaceae), Simkania (family Simkaniaceae) and Estrella (family Criblamydiaceae); additionally, DNA from the genus Thermoanaerobacter (family Thermoanaerobacteriaceae) was detected. A global autocorrelation analysis did not reveal a spatial structure in the observed CLOs infection rates, and association tests involving land cover characteristics did not evidence any clear effect on CLOs infection rates in B. bufo. Despite preliminary, these results suggest a random and ubiquitous distribution of CLOs in the environment, which would support the biogeographical expectation “everything is everywhere” for the concerned microorganisms and their amoeba vectors.
Trisomy 21 activates the kynurenine pathway via increased dosage of interferon receptors
Authors: Powers Rani K. , Sullivan Kelly D. , Culp-Hill Rachel , Ludwig Michael P. , Smith Keith P. , Waugh Katherine A. , Minter Ross , Tuttle Kathryn D. , Lewis Hannah C. , Rachubinski Angela L. , Granrath Ross E. , Wilkerson Rebecca B. , Kahn Darcy E. , Joshi Molishree , D’Alessandro Angelo , Costello James C. , Espinosa Joaquin M. 
Published: August 2018

ABSTRACTTrisomy 21 (T21) causes Down syndrome (DS), affecting immune and neurological function by unknown mechanisms. We report here the results of a large metabolomics study showing that people with DS produce elevated levels of kynurenine and quinolinic acid, two tryptophan catabolites with potent immunosuppressive and neurotoxic properties, respectively. We found that immune cells of people with DS overexpress IDO1, the rate-limiting enzyme in the kynurenine pathway (KP) and a known interferon (IFN)-stimulated gene. Furthermore, we found a positive correlation between levels of specific inflammatory cytokines and KP dysregulation. Using metabolic flux assays, we found that IFN stimulation causes IDO1 overexpression and kynurenine overproduction in cells with T21, dependent on overexpression of IFN receptors encoded on chromosome 21. Finally, KP dysregulation is conserved in a mouse model of DS carrying triplication of the IFN receptors. Altogether, these results reveal a mechanism by which T21 could drive immunosuppression and neurotoxicity in DS.
Optimization of swelling response of pH sensitive hydrogels using Box-Behnken design
Authors: Shokri Fereshteh , Ebrahimi Pouneh , Honary Soheyla 
Published: August 2018

AbstractHydrogels are three-dimensional crosslinked hydrophilic polymer networks capable of swelling or de-swelling reversibly in water and retaining a large volume of liquid in swollen state. Hydrogels can be designed with controllable responses to shrink or expand with changes in external environmental conditions. Among stimulisensitive hydrogels, the pH-sensitive ones are widely-studied and used. Despite these advances, we currently lack a systematic way to optimize the synthesis protocols. Here we propose to use techniques from chemometrics, namely Box–Behnken statistical screening design to optimize the chemical composition of pH-responsive hydrogels for an improved responsiveness as quantified by swelling index. Polymer blends were prepared by mixing different suitable volume of pvp (poly vinyl pyrrolidone) and chitosan aqueous solution in order to obtain a mixture, glutardialdehyde solution was added as crosslinkers to the chitosan/pvp mixture to form semi-IPN(semi-interpenetrating polymeric hydrogel). The pH-dependent swelling properties have been measured and used to obtain a regression model. We characterized the descriptive and predictive abilities of our model. We found a remarkable correlation (correlation coefficient = 0.954) between the observed responses and the responses predicted by the model. Our results demonstrate that Box-Behnken is an appropriate statistical design that can be successfully used in the development of pH-sensitive hydrogels with a predictable swelling ratio. This technology will remarkably reduce the time and cost that is needed for chemical synthesis of hydrogels with a desired pH-sensitivity.
Differential gene expression indicates involvement of F-box proteins and E3 ligases in sexualversusapomictic germline specification inBoechera
Authors: Zuehl Luise , Ibberson David , Schmidt Anja 
Published: August 2018

ABSTRACTGermline specification is the first step during sexual and apomictic plant reproduction. This takes place in a specialized domain of the reproductive flower tissues, the nucellus of the ovule. In each case, a sporophytic cell is determined to initiate germline development. These cells, the megaspore mother cell (MMC) or apomictic initial cell (AIC) in sexual plants and apomicts, respectively, differ in their developmental fate. While the MMC undergoes meiosis, the AIC aborts or omits meiosis to form the female gametophyte. Although these distinct developmental processes have long been described, little is known about the gene regulatory basis involved.To elucidate gene regulatory networks underlying sexual and apomictic germline specification, we conducted tissue-specific transcriptional profiling using laser-assisted microdissection and RNA-Seq. We compared the transcriptomes of the nucellar tissues harbouring the MMC or AIC between different accessions ofBoechera. The six accessions we used represented four species and two ploidy levels, allowing us to distinguish between differences in gene expression caused by the genetic background or the reproductive mode.Comparative data analysis revealed widely overlapping gene expression patterns in apomicticversussexualBoecheraaccessions. Nevertheless, 45 significantly differentially expressed genes were identified, which potentially play a role for determination of sexualversusapomictic reproductive mode. Interestingly, based on annotations, these include F-box proteins and E3 ligases that might relate to genes previously described as regulators important for sexual or apomictic reproduction. Thus, our findings provide new insight into the transcriptional basis of sexual and apomictic germline specification.One sentence summaryA comprehensive tissue type-specific transcriptional analysis using laser-assisted microdissection combined with RNA-Seq identifies 45 genes consistently differentially expressed during germline specification in different sexualversusapomicticBoecheraaccessions, indicating roles of protein degradation related to cell cycle, transcriptional and post-transcriptional regulatory processes, and stress response for apomixis.
Does the strength of women’s attraction to male vocal masculinity track changes in steroid hormones?
Authors: Jones Benedict C , Hahn Amanda C , Pisanski Katarzyna , Wang Hongyi , Kandrik Michal , Lee Anthony J , Holzleitner Iris J , Feinberg David R , DeBruine Lisa M 
Published: August 2018

AbstractRecent studies that either used luteinizing hormone tests to confirm the timing of ovulation or measured steroid hormones from saliva have found little evidence that women’s preferences for facial or body masculinity track within-subject changes in women’s fertility or hormonal status. Fewer studies using these methods have examined women’s preferences for vocal masculinity, however, and those that did report mixed results. Consequently, we used a longitudinal design and measured steroid hormones from saliva to test for evidence of hormonal regulation of women’s (N=351) preferences for two aspects of male vocal masculinity (low pitch and low formants). Analyses suggested that preferences for masculine pitch, but not masculine formants, may track within-woman changes in estradiol. Although these results present some evidence for the hypothesis that within-subject hormones regulate women’s attraction to masculine men, we do not discount the possibility that the effect of estradiol on pitch preferences in the current study is a false positive.
Signature patterns for top-down and bottom-up information processing via cross-frequency coupling in macaque auditory cortex
Authors: Márton Christian D. , Fukushima Makoto , Camalier Corrie R. , Schultz Simon R. , Averbeck Bruno B. 
Published: August 2018

AbstractPredictive coding is a theoretical framework that provides a functional interpretation of top-down and bottom up interactions in sensory processing. The theory has suggested that specific frequency bands relay bottom-up and top-down information (e.g. “γup,βdown”). But it remains unclear whether this notion generalizes to cross-frequency interactions. Furthermore, most of the evidence so far comes from visual pathways. Here we examined cross-frequency coupling across four sectors of the auditory hierarchy in the macaque. We computed two measures of cross-frequency coupling, phase-amplitude coupling (PAC) and amplitude-amplitude coupling (AAC). Our findings revealed distinct patterns for bottom-up and top-down information processing amongcross-frequency interactions. Both top-down and bottom-up made prominent use of low frequencies: low-to-low frequency (θ, α, β) and low frequency-to-high γcouplings were predominanttop-down, while low frequency-to-low γcouplings were predominantbottom-up. These patterns were largely preserved across coupling types (PAC and AAC) and across stimulus types (natural and synthetic auditory stimuli), suggesting they are a general feature of information processing in auditory cortex. Moreover, our findings showed that low-frequency PAC alternated between predominantly top-down or bottom-up over time. Altogether, this suggests sensory information need not be propagated along separate frequencies upwards and downwards. Rather, information can be unmixed by having low frequencies couple to distinct frequency ranges in the target region, and by alternating top-down and bottom-up processing over time.1SignificanceThe brain consists of highly interconnected cortical areas, yet the patterns in directional cortical communication are not fully understood, in particular with regards to interactions between different signal components across frequencies. We employed a a unified, computationally advantageous Granger-causal framework to examine bi-directional cross-frequency interactions across four sectors of the auditory cortical hierarchy in macaques. Our findings extend the view of cross-frequency interactions in auditory cortex, suggesting they also play a prominent role in top-down processing. Our findings also suggest information need not be propagated along separate channels up and down the cortical hierarchy, with important implications for theories of information processing in the brain such as predictive coding.
Peripartum cardiomyopathy and hypertensive disorders of pregnancy and cardiovascular events among 1.6 million California pregnancies
Authors: Arnaout Rima , Nah Gregory , Marcus Gregory M. , Tseng Zian H. , Foster Elyse , Harris Ian , Divanji Punag , Klein Liviu , Gonzalez Juan M. , Parikh Nisha I. 
Published: August 2018

AbstractBackgroundCardiovascular complications during and soon after pregnancy present an opportunity to assess risk for subsequent cardiovascular disease. We sought to determine whether peripartum cardiomyopathy and hypertensive disorder of pregnancy subtypes predict future myocardial infarction, heart failure, or stroke independent of one another and independent of other risks like gestational diabetes, preterm birth, and intrauterine growth restriction.Methods and ResultsThe California Healthcare Cost and Utilization Project database was used to identify all hospitalized pregnancies from 2005-2009, with follow-up through 2011, for a retrospective cohort study. Pregnancies, exposures, covariates and outcomes were defined by ICD-9 codes. Among 1.6 million pregnancies (mean age 28y; median follow-up time to event 2.7y), 558 cases of peripartum cardiomyopathy, 123,603 cases of hypertensive disorders of pregnancy, 107,636 cases of gestational diabetes, 116,768 preterm births, and 23,504 cases of intrauterine growth restriction were observed. Using multivariable Cox proportional hazards models, peripartum cardiomyopathy was independently associated with a 13.0-fold increase in myocardial infarction [95%CI, 4.1-40.9], a 39.2-fold increase in heart failure [95%CI, 30.0-51.9], and a 7.7-fold increase in stroke [95%CI, 2.4-24.0]. Hypertensive disorders of pregnancy were associated with a 1.4 [95%CI, 1.0-2.0] to 7.6 [95%CI, 5.4-10.7] fold higher risk of myocardial infarction, heart failure, and stroke. Gestational diabetes, preterm birth, and intrauterine growth restriction had more modest associations with CVD.ConclusionsThese findings support close monitoring of women with cardiovascular pregnancy complications for prevention of early subsequent cardiovascular events and further study of mechanisms underlying their development.
The Role of Toll-like receptor 4 in respiratory syncytial virus replication, interferon lambda 1 induction, and chemokine responses
Authors: Broadbent Lindsay , Coey Jonathon D. , Shields Michael D. , Power Ultan F. 
Published: August 2018

AbstractRespiratory syncytial virus (RSV) infection is the leading cause of severe lower respiratory tract infections (LRTI) in infants worldwide. The immune responses to RSV infection are implicated in RSV pathogenesis but RSV immunopathogenesis in humans remains poorly understood. We previously demonstrated that IFN-λ1 is the principle interferon induced following RSV infection of infants and well-differentiated primary pediatric bronchial epithelial cells (WD-PBECs). Interestingly, RSV F interacts with the TLR4/CD14/MD2 complex to initiate secretion of pro-inflammatory cytokines, while TLR4 stimulation with house dust mite induces IFN-λ1 production. However, the role of TLR4 in RSV infection and concomitant IFN-λ1 induction remains unclear. Using our RSV/WD-PBEC infection model, we found that CLI-095 inhibition of TLR4 resulted in significantly reduced viral growth kinetics, and secretion of IFN-λ1 and pro-inflammatory chemokines. To elucidate specific TLR4 signalling intermediates implicated in virus replication and innate immune responses we selected 4 inhibitors, including LY294002, U0126, SB203580 and JSH-23. SB203580, a p38 MAPK inhibitor, reduced both viral growth kinetics and IFN-λ1 secretion, while JSH-23, an NF-κB inhibitor, reduced IFN-λ1 secretion without affecting virus growth kinetics. Our data indicate that TLR4 plays a role in RSV entry and/or replication and IFN-λ1 induction following RSV infection is mediated, in part, by TLR4 signalling through NF- κB and/or p38 MAPK. Therefore, targeting TLR4 or downstream effector proteins could present novel treatment strategies against RSV.ImportanceThe role of TLR4 in RSV infection and IFN-λ1 induction is controversial. Using our WD-PBEC model, which replicates many hallmarks of RSV infection in vivo, we demonstrated that the TLR4 pathway is involved in both RSV infection and/or replication and the concomitant induction of IFN-λ1 and other pro-inflammatory cytokines. Increasing our understanding of the role of TLR4 in RSV immunopathogenesis may lead to the development of novel RSV therapeutics.
Benchmark Problems for Dynamic Modeling of Intracellular Processes
Authors: Hass Helge , Loos Carolin , Alvarez Elba Raimundez , Timmer Jens , Hasenauer Jan , Kreutz Clemens 
Published: August 2018

AbstractMotivationDynamic models are used in systems biology to study and understand cellular processes like gene regulation or signal transduction. Frequently, ordinary differential equation (ODE) models are used to model the time and dose dependency of the abundances of molecular compounds as well as interactions and translocations. A multitude of computational approaches have been developed within recent years. However, many of these approaches lack proper testing in application settings because a comprehensive set of benchmark problems is yet missing.ResultsWe present a collection of 20 ODE models developed given experimental data as benchmark problems in order to evaluate new and existing methodologies, e.g. for parameter estimation or uncertainty analysis. In addition to the equations of the dynamical system, the benchmark collection provides experimental measurements as well as observation functions and assumptions about measurement noise distributions and parameters. The presented benchmark models comprise problems of different size, complexity and numerical demands. Important characteristics of the models and methodological requirements are summarized, estimated parameters are provided, and some example studies were performed for illustrating the capabilities of the presented benchmark collection.AvailabilityThe models are provided in several standardized formats, including an easy-to-use human readable form and machine-readable SBML files. The data is provided as Excel sheets. All files are available athttps://github.com/Benchmarking-Initiative/Benchmark-Models, with MATLAB code to process and simulate the models.Contactjan.hasenauer@helmholtz-muenchen.de,ckreutz@fdm.uni-freiburg.de