bluuebunny/crossref_metadata_2025_split huggingface dataset:
Frequency, impact and a preclinical study of novel ERBB gene family mutations in HER2-positive breast cancer
Authors: Elster Naomi , Toomey Sinead , Fan Yue , Cremona Mattia , Morgan Clare , Weiner Gorzel Karolina , Bhreathnach Una , Milewska Malgorzata , Murphy Madeline , Madden Stephen , Naidoo Jarushka , Fay Joanna , Kay Elaine , Carr Aoife , Kennedy Sean , Furney Simon , Mezynski Janusz , Breathhnach Oscar , Morris Patrick , Grogan Liam , Hill Arnold , Kennedy Susan , Crown John , Gallagher William , Hennessy Bryan , Eustace Alex 
Published: July 2018

Background: Somatic mutations in the ERBB genes (epidermal growth factor receptor: EGFR, ERBB2, ERBB3, ERBB4) promote oncogenesis and lapatinib resistance in metastatic HER2+ (human epidermal growth factor-like receptor 2) breast cancer in vitro. Our study aimed to determine the frequency of mutations in four genes: EGFR, ERBB2, ERBB3 and ERBB4 and to investigate whether these mutations affect cellular behaviour and therapy response in vitro and outcomes after adjuvant trastuzumab-based therapy in clinical samples. Methods: We performed Agena MassArray analysis of 227 HER2+ breast cancer samples to identify the type and frequency of ERBB family mutations. Of these, two mutations, the somatic mutations ERBB4-V721I and ERBB4-S303F, were stably transfected into HCC1954 (PIK3CA mutant), HCC1569 (PIK3CA wildtype) and BT474 (PIK3CA mutant, ER positive) HER2+ breast cancer cell lines for functional in vitro experiments. Results: A total of 12 somatic, likely deleterious mutations in the kinase and furin-like domains of the ERBB genes (3 EGFR, 1 ERBB2, 3 ERBB3, 5 ERBB4) were identified in 7% of HER2+ breast cancers, with ERBB4 the most frequently mutated gene. The ERBB4-V721I kinase domain mutation significantly increased 3D-colony formation in 3/3 cell lines, whereas ERBB4-S303F did not increase growth rate or 3D colony formation in vitro. ERBB4-V721I sensitized HCC1569 cells (PIK3CA wildtype) to the pan class I PI3K inhibitor copanlisib but increased resistance to the pan-HER family inhibitor afatinib. The combinations of copanlisib with trastuzumab, lapatinib, or afatinib remained synergistic regardless of ERBB4-V721I or ERBB4-S303F mutation status. Conclusions: ERBB gene family mutations, which are present in 7% of our HER2+ breast cancer cohort, may have the potential to alter cellular behaviour and the efficacy of HER- and PI3K-inhibition.
The Dermatophagoides pteronyssinus Molecular Sensitization Profile of Allergic Rhinitis Patients in Central China
Authors: Yang Yongshi , Zhu Rongfei , Huang Nan , Li Wenjing , Zhang Wei , Wang Yin , Yang Lin 
Published: July 2018

Background House dust mites (HDMs) are the major aeroallergens in patients with rhinitis and/or asthma in China. However, the molecular sensitization of HDM is varied in different regions. Objective To investigate the Dermatophagoides pteronyssinus ( Der p) molecular sensitization profile of allergic rhinitis (AR) patients in Central China. Methods AR patients with positive skin prick tests to Der p were enrolled in our study. Specific immunoglobulin E (sIgE) for Der p, Dermatophagoides farinae ( Der f), Acarus siro ( Aca s), Blomia tropicalis ( Blo t), and Der p components Der p1, Der p2, and Der p10 were measured in all patients. Results A total of 130 patients were included. The overall prevalence of positive serum sIgE was 99.2% for Der p, 98.5% for Der f, 81.5% for Aca s, 83.1% for Blo t, 71.5% for Der p1, 64.6% for Der p2, and 11.5% for Der p10. HDM-AR patients with asthma displayed significantly higher concentrations of sIgE to Der p, Der f, Der p1, and Der p2 than did those without asthma ( P < .001). The prevalence of asthma in HDM-AR patients was higher among the patients sensitized to both Der p1 and Der p2 (62.8%) than those sensitized to only 1 allergen ( Der p1 26.7% or Der p2 16.7%; P < .05) or nonsensitized to Der p1 and Der p2 (19.4%; P < .001). Conclusion Der p has high cross-reactivity with other mite species. Der p1 and Der p2 are the major components to induce Der p sensitization among AR patients in Central China. Sensitization to both Der p1 and Der p2 may be a risk factor for developing asthma in HDM-AR patients.
The patient burden of opioid‐induced constipation: New insights from a large, multinational survey in five European countries
Authors: Andresen Viola , Banerji Vivek , Hall Genevieve , Lass Amir , Emmanuel Anton V 
Published: July 2018

BackgroundDespite its high prevalence, opioid‐induced constipation (OIC) remains under‐recognised and undertreated, and its true impact on wellbeing and quality of life (QoL) may be underestimated.MethodsA quantitative, questionnaire‐based international survey was conducted.ResultsWeak‐opioid users appeared as bothered by constipation as strong‐opioid users (38% vs 40%, respectively; p = 0.40), despite it causing less‐severe physical symptoms and impact on QoL. Strong‐opioid users meeting Rome IV OIC criteria appeared to experience greater symptomatic and biopsychosocial burden from constipation than those not satisfying these criteria. Almost one‐fifth of respondents were dissatisfied with their current constipation treatment and around one‐third found balancing the need for adequate pain relief with constipation side effects challenging. Consequently, more than half failed to adhere to their prescribed treatment regimens, or resorted to suboptimal strategies, e.g. 40% reduced their opioid intake, to relieve constipation. Almost 60% of healthcare professionals did not adequately counsel patients about constipation as a common side effect of opioid use.ConclusionsFindings suggest that both weak‐ and strong‐opioid users suffer comparable bother and decreased QoL, Rome IV criteria can identify patients with more‐severe OIC, but may underdiagnose patients showing fewer symptoms, and increased education is needed to manage patients’ expectations and enable improved OIC self‐management.
Socioeconomic Determinants, Maternal Health, and Caries in Young Children
Authors: Julihn A. , Soares F.C. , Hjern A. , Dahllöf G. 
Published: July 2018

Introduction: Maternal health during pregnancy plays a part in child health, and several conditions have been associated with adverse child outcomes. Objectives: To determine the socioeconomic determinants and maternal health factors associated with dental caries in young children. Methods: This cross-sectional study is part of a register-based cohort study including all children who were born from 2000 to 2003 and were residing in Stockholm County, Sweden, at age 3 y ( n = 73,658). The study followed the cohort until individuals were 7 y old. The final study cohort comprised all children examined at 3 and 7 y ( n = 65,259). Data on socioeconomic conditions, maternal health, and maternal health behavior were extracted from Swedish national registries. The multivariate analyses used 2 outcomes: caries experience at age 3 and 7 y (deft > 0 [decayed, extracted, and filled teeth]). Results: The results of this study show that socioeconomic and maternal health behaviors during pregnancy are important determinants of oral health in their preschool offspring. When all significant risk factors were present, the cumulative probability of being diagnosed with dental caries at age 7 y was 75%. Conclusion: This study also showed that maternal obesity and smoking during pregnancy were predictors of dental caries in preschool children. Strategies must be developed for increasing maternal motivation and self-efficacy and providing mothers with knowledge and caries-preventive tools. Knowledge Transfer Statement: The results of this study inform clinicians about the importance of including a more detailed history regarding maternal health and maternal health behaviors during pregnancy to assess caries risk in preschool children. Education, income, and other socioeconomic factors are difficult to modify in the short term. Therefore, strategies must be developed to increase parental motivation and self-efficacy to give parents the determination, knowledge, and tools for prevention.
Tracheostomy reversal years after patient lost to follow-up
Authors: Querney Jill , Ethier Jean-Luc 
Published: July 2018

Background Pediatric tracheostomies occur for various reasons, including prologned intubation, and require a multidisciplinary approach with routine follow-up. Case presentation This report reviews the history and clinical outcome of a 29 year old female patient who was lost to follow-up for nearly two decades after a pediatric tracheostomy. When she presented to the Otolaryngology service as an adult the original indication for tracheostomy had resolved and decannulation was initiated, but a profound psychological dependence had developed. Conclusion This case outlines the importance of regular follow-up for tracheostomy patients, as well as health care barriers faced in remote rural communities.
Puzzles of Creek Social Organization in the Eighteenth and Nineteenth Centuries
Authors: Knight Vernon James 
Published: July 2018

Abstract
Despite intensive study by John R. Swanton, in the early twentieth century, of social organization in the towns of the early Creek Confederacy, we are left with certain puzzling features. The article outlines two of them. First, despite the remarkable attention paid to clans and clanship, the nature of local, corporate kin groups was never clearly resolved. Second, despite evidence of a strong separation between the matrilineal organization, on the one hand, and the town council on the other, Swanton gives numerous examples where the two seem impossibly commingled. The article offers thoughts toward resolving both puzzles.
“My Home Is on Both Sides”: Indigenous Communities and the US-Canadian Border on the Columbia Plateau, 1880s–1910s
Authors: Lozar Patrick 
Published: July 2018

Abstract
For indigenous groups inhabiting the interior Pacific Northwest’s Columbia Plateau, issues of native group identity took on a transnational dimension with the imposition of the US-Canadian border in the late nineteenth and early twentieth centuries. This article examines how the Okanagan, Sinixt, and Ktunaxa peoples, whose homelands and communities were bifurcated by the forty-ninth parallel, negotiated and complicated Canada’s and the United States’ enforcement of the international boundary. These partitioned indigenous communities endeavored to maintain cultural connections to each other across the line while also engaging their recognized political status within the bounds of either nation-state. Focusing on the activities of Native Plateau peoples in the borderlands reestablishes the persistence of indigenous articulations of territory and nationhood that transcended the US-Canadian border.
What's new (and not so new) about sheep scab
Authors: Busin Valentina 
Published: July 2018

Sheep scab represents a significant burden for the national sheep industry and a serious welfare issue for diseased animals. Although different treatment options are available and despite tackling of this disease being a top priority for the industry, sheep scab is currently widely spread within the UK, and its control still represents a significant challenge for all the parties involved. This article provides an overview of sheep scab, focusing on the established knowledge available for both clinical and subclinical disease. Recent advancements in the diagnosis of the disease are also presented, with an emphasis on the added value and practical use of a blood test for diagnosis of the subclinical disease, and for its use in monitoring flock exposure to the parasite. Currently available and future options for treatment and disease control are discussed, especially in the light of the challenge posed by the development of drug resistance.
Charging, accessibility and legitimacy: a case study of a third sector ‘arts and health’ charity
Authors: Swan Peter 
Published: July 2018

With statutory income becoming scarcer or more restricted, many third sector organisations have considered charging clients to make up shortfalls in funding. However, many organisations work with some of the poorest people in society, who may be incapable of contributing financially towards the cost of accessing the service they provide. Using the case study of ‘Artspace’, a community ‘arts and health’ charity, this article investigates, with particular reference to Goffman’s theory of ‘impression management’, how organisations can maintain legitimacy with their beneficiaries while implementing potentially unpopular decisions.
Artspace initially utilised ‘excuses’ and ‘justifications’ when consulting with clients about the need for additional fees. However, the somewhat fragile nature of the relationship between the organisation and the community necessitated a wider, more bespoke range of strategies. Artspace was able to successfully maintain legitimacy with beneficiaries largely because of its perceived and actual capability of responding to challenges in a timely, user-centred and innovative manner.
EVALUATION OF THE LEVEL OF SATISFACTION OF HOSPITALIZED PUERPERAL WOMAN IN A MATERNITY IN THE COUNTRYSIDE OF PARAÍBA
Authors: Freitas Maria Cristina Nascimento de , Santos Joselito , Sousa Andreia Oliveira Barros , Cabral Symara Abrantes Albuquerque de Oliveira , Alves Maria Nizete Tavares , Barbosa Jailma Dos Santos , Lima Danielly Gonçalves Sombra 
Published: July 2018

There is now a growing need for improvement in health services provided in all health institutions. One possible strategy with this aim is to identify factors that may directly interfere with the quality of care and, therefore, determine the satisfaction of the user of this service . The purpose of this study was to evaluate the level of satisfaction of postpartum women admitted to a maternity ward, taking into account the existing structure and services provided by the institution. This is an exploratory, descriptive research of quantitative nature developed at the Deodato Cartaxo maternity hospital, belonging to the Regional Hospital of Cajazeiras, located in the municipality of Cajazeiras, Paraiba State in Brazil. The population was composed of hospitalized puerperal women and the sample of 100 woman was performed for convenience, systematic of the proportional type with a sample error of 5%. The data collection period was between the months of august and september 2015. The instrument of data collection was SERVQUAL, evaluating its five quality indicators (tangibility, reliability, attendance, safety and empathy). The results were presented in table form with their respective absolute and relative frequencies. Among the indicators studied, reliability, care and security were the ones that generated the most satisfaction among the puerperal woman and tangibility was referred to as an indicator of dissatisfaction among the users. Keywords: Evaluation of health services; consumer satisfaction; maternities.
Tahâvî Bağlamında İki Farklı Hanefîlik Okuması (Ekmelüddîn El-Bâbertî Ve İbn Ebi’l-İzz Örneği)
Authors: AYKAÇ Mustafa 
Published: July 2018

Hem Müslümanın günlük hayatında karşılaşabileceği sorunlara makul ve pratik fıkhî çözümler getirmesi, hem de kucaklayıcı itikâdî görüşleri ile Ebû Hanîfe (ö.150/M.767), sadece mevâlinin değil tüm Müslümanlar katında önemli bir âlim olmuştur. Onun görüşlerinin Mısır bölgesinde takipçisi ve belirgin ilk temsilcisi İmam Tahâvî’dir (ö.321/M.933). Bu çalışmada İmam Tahavî üzerinden Mısır’daki Hanefîlik algısının izlerini bulmayı hedeflemekteyiz. Bu hedef doğrultusunda çalışmamız, Ekmelüddîn el-Bâbertî (ö.786/M.1384) ile İbn Ebi’l-İzz’in (ö.792/M.1390) İmam Tahavî’nin el-Akîdetü’t-Tahâviyye veya Beyanü Akîdeti Ehli’s-Sünne ve’l-Cemaa isimli eseri üzerine yazdığı şerhlerde ortaya koyulan Hanefîlik algısı üzerinden şekillenecektir. Ancak çalışmamızın makale sınırlarını aşmaması için, söz konusu âlimlerin iman görüşleri etrafında bir soruşturma yapılacaktır. Bu hedef doğrultusunda Ekmelüddîn el-Bâbertî ve İbn Ebi’l-İzz’in şerhlerine yansıttığı gelenek tespit edilecek ve Hanefîliğe dair farklı sunumlarının sebebi araştırılacaktır.
Perbandingan Nilai Nutrisi pada Beberapa Spesies Tumbuhan Akuatik Perairan Rawa Pening
Authors: Izzati Munifatul 
Published: July 2018

Tumbuhan akuatik sering tumbuh melimpah di ekosistem perairan. Pertumbuhannya yang cepat menyebabkan pendominasian di kawasan perairan. Oleh karena itu diperlukan upaya untuk memanfaatkannya, sehingga populasinya dapat dikendalikan. Penelitian ini bertujuan untuk menguji nilai nutrisi dari beberapa spesies tumbuhan akuatik ditinjau dari berat kering, kadar protein, lemak dan karbohidrat. Sebanyak 9 spesies tumbuhan akuatik dikoleksi dari beberapa titik, di perairan Rawa Pening, Jawa Tengah. Spesies tumbuhan yang sama dicampur, dikeringkan, dan ditumbuk hingga halus. Tepung yang dihasilkan selanjutnya dianalisis berat kering, kandungan protein, lemak dan karbohidratnya melalui analisis proksimat secara duplo. Data yang diperoleh dibuat reratanya dan disajikan dalam grafik. Hasil penelitian menunjukkan bahwa berat kering tertinggi dihasilkan oleh Myriophyllum siberesum, kandungan protein tertinggi dihasilkan oleh Ceratophyllum demersum, kandungan lemak tertinggi dihasilkan oleh Pistia stratiotes, sedangkan kandungan karbohidrat tertinggi dihasilkan oleh Salvinia molesta. Tumbuhan akuatik dengan kandungan protein, lemak dan karbohidrat yang tinggi mempunyai potensi untuk dikembangkan menjadi pakan. Kata kunci: tumbuhan akuatik, protein, lemak, karbohidrat
Variasi Kandungan Nutrien dalam Tambak Wanamina dengan Komposisi Jenis dan Jumlah Tegakan Mangrove yang Berbeda di Pesisir Kota Semarang
Authors: Hastuti Endah Dwi , Budihastuti Rini 
Published: July 2018

Keberadaan vegetasi mangrove dalam tambak wanamina berfungsi sebagai penyedia jasa lingkungan, diantaranya dalam pengendalian suplai nutrien ke dalam kolam. Penelitian ini bertujuan untuk mengkaji variasi kandungan nutrien dalam tambak wanamina dan menguji pengaruh jenis serta jumlah tegakan terhadap perubahan kandungan nutrien tambak wanamina. Penelitian dilaksanakan selama 4 bulan dengan perlakuan jenis vegetasi (A. marina; R. mucronata; Campuran) dan jumlah tegakan mangrove (5 tegakan; 10 tegakan; 15 tegakan). Kandungan nutrien yang diamati meliputi bahan organik, nitrat, nitrogen dan fosfat terlarut. Pengamatan dilakukan sebanyak 2 kali dengan jeda waktu 1 bulan. Pengujian dilakukan dengan uji-t dan ANOVA. Hasil pengamatan menunjukkan bahwa terdapat variasi kandungan nutrien dalam tambak wanamina baik pada pengamatan pertama maupun kedua. Pola perubahan kandungan nutrien bervariasi antar jenis nutrien dimana pada beberapa perlakuan ditemukan penurunan kandungan semua jenis nutrien. Hasil uji-t menunjukkan bahwa antar periode pengamatan tidak terdapat perbedaan yang signifikan terhadap kandungan bahan organik, nitrat, nitrogen dan fosfat terlarut. Hasil ANOVA menunjukkan bahwa tidak terdapat pengaruh yang signifikan terhadap kandungan nutrien dalam tambak wanamina baik pada pengamatan pertama maupun kedua. Perbedaan kandungan nutrien yang signifikan hanya diperoleh pada kandungan bahan organik pada pengamatan pertama. Kata kunci: jenis, jumlah tegakan, mangrove, nutrien, wanamina
Pengaruh Pemberian Tepung Daun Jati Belanda (Guazuma ulmifolia L.) dalam Pakan terhadap Kadar Trigliserida Darah dan Lemak Abdominal Ayam Broiler
Authors: Budiarto Mohammad Arif , Yuniwarti Enny Yusuf Wachidah , - Isroli 
Published: July 2018

Ayam broiler dikenal mempunyai lemak tubuh tinggi, sehingga perlu upaya penurunan lemak dengan menggunakan bahan alami (herbal) sebagai pakan tambahan. Penggunaan daun jati belanda diharapkan mampu menurunkan lemak ayam broiler. Penelitian ini bertujuan untuk mengkaji khasiat tepung daun jati belanda dalam menurunkan kadar trigliserida darah dan persentase lemak abdominal ayam broiler. Penelitian ini menggunakan 100 ekor DOC (day old chicks) betina. Penelitian ini merupakan penelitian eksperimental laboratorium dengan rancangan acak lengkap (RAL) menggunakan 5 perlakuan dan 5 ulangan pada setiap perlakuan dan jika ada beda nyata antar perlakuan dilakukan uji lanjut dengan uji beda nyata terkecil (BNT) dengan tingkat kepercayaan 95 %. Pengujian pengaruh penurunan kadar trigliserida darah dan lemak abdominal dilakukan selama 28 hari perlakuan. Hasil penelitian menunjukkan bahwa pemberian tepung daun jati belanda (Guazuma ulmifolia Lamk.) pada dosis 5g, 10g, 15 g dan 20 g dalam 1 kg pakan komplit mampu menurunkan kadar trigliserida darah dan lemak abdominal ayam broiler ((P<0.05) dibanding dengan kontrol. Kata kunci : daun jati belanda, ayam broiler, trigliserida darah, lemak abdominal
Pertumbuhan Puyuh (Coturnix coturnix japonica L.) Setelah Pemeliharaan dengan Cahaya Monokromatik
Authors: Winata Norma , Praseno Koen , Tana Silvana 
Published: July 2018

Puyuh (Coturnix coturnix japonica L.) merupakan salah satu jenis aves yang memiliki produktivitas tinggi dengan masa pemeliharaan yang relatif singkat. Puyuh memiliki dua fase pemeliharaan, yaitu fase pertumbuhan dan fase produksi. Pertumbuhan puyuh dipengaruhi oleh faktor internal dan eksternal. Cahaya merupakan salah satu faktor eksternal yang mempengaruhi pertumbuhan aves. Proses pertumbuhan seperti pertambahan bobot dan ukuran tubuh serta pola tingkah laku makan dipengaruhi oleh sistem hormonal yang diregulasi oleh cahaya. Penelitian ini bertujuan melihat potensi cahaya monokromatik terhadap pertumbuhan puyuh. Metode yang digunakan adalah pencahayaan monokromatik sebagai perlakuan dalam 3 warna, yaitu merah, hijau, dan biru, serta kontrol sebagai pembanding menggunakan lampu pijar. Perlakuan dimulai pada saat puyuh berusia 4 minggu sampai 12 minggu. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL). Data yang dihasilkan dalam penelitian ini dianalisis menggunakan Analysis of Varian (ANOVA), karena terdapat perbedaan nyata maka dilanjutkan dengan Uji Duncan pada taraf kepercayaan 95%. Parameter dalam penelitian ini adalah bobot tubuh, pertambahan bobot tubuh, konsumsi pakan, dan konversi pakan. Hasil penelitian ini menunjukkan adanya perbedaan nyata pada bobot tubuh puyuh dan pertambahan bobot tubuh dari perlakuan cahaya monokromatik terhadap kontrol. Simpulan dari penelitian ini adalah pencahayaan dengan cahaya monokromatik warna hijau lebih berpotensi meningkatkan pertumbuhan puyuh. Kata kunci : puyuh (Coturnix coturnix japonica L.), pertumbuhan, cahaya monokromatik
Performa Green Bean Kopi Robusta (Coffea robusta Lindl.Ex De Will) setelah Perendaman Limbah Tahu dengan Jenis dan Konsentrasi yang Berbeda
Authors: Sulistyaningtyas Ayu Rahmawati , Prihastanti Erma , Hastuti Endah Dwi 
Published: July 2018

Tingkat konsumsi terhadap kopi robusta lebih rendah dari pada kopi arabika karena performanya kurang baik. Salah satu upaya peningkatan kualitas performa kopi melalui dekafeinasi. Kopi dekafeinasi yang telah dipasarkan secara luas misalnya kopi luwak. Prinsip pembuatan kopi luwak melibatkan protease dalam pencernaan hewan luwak. Dekafeinasi menggunakan protease tersebut telah terbukti menurunkan kadar kafein kopi robusta sehingga performanya meningkat. Sumber protease dapat diperoleh dari limbah tahu. Dekafeinasi dengan cara merendam green bean kopi robusta (Coffea. robusta) dalam limbah tahu belum pernah dilakukan. Tujuan penelitian antara lain mengkaji pengaruh jenis limbah tahu dan konsentrasi limbah tahu serta interaksi jenis dan konsentrasi limbah industri tahu terhadap performa pada kopi robusta. Penelitian ini menggunakan Rancangan Acak Lengkap faktorial dengan 2 faktor yaitu faktor jenis limbah tahu (L0= Air, L1=limbah cair dan L2=limbah padat) dan faktor konsentrasi limbah tahu (K1=30%, K2=60% dan K3=90%), setiap perlakuan dilakukan 3 ulangan. Parameter penelitian yang diamati adalah performa kopi robusta meliputi warna, aroma dan tekstur green bean kopi robusta. Analisis data yang digunakan adalah Analisis of Variance (ANOVA) yang dilanjutkan dengan Duncan Multiple Range Test (DMRT) pada taraf signifikasi 95 %. Hasil penelitian menunjukkan bahwa perendaman kopi robusta dalam limbah cair tahu 90 % paling efektif meningkatkan kualitas performa yang meliputi warna, aroma dan tekstur green bean. Kata kunci : Coffea robusta, dekafeinasi, kopi, limbah tahu, performa