bluuebunny/crossref_metadata_2025_split huggingface dataset:
Decrease of AIM2 mediated by luteolin contributes to non-small cell lung cancer treatment
Authors: Yu Qian , Zhang Minda , Ying Qidi , Xie Xin , Yue Shuwen , Tong Bending , Wei Qing , Bai Zhaoshi , Ma Lingman 
Published: March 2019

AbstractNon-small cell lung cancer (NSCLC) is one of the most common malignancies in the world. Although extensive studies showed that luteolin exhibited antitumor effects against NSCLC, the mechanism has not been fully established. In the present study, we found that luteolin significantly reduced the expression of absent in melanoma 2 (AIM2) at both mRNA and protein levels leading to the suppression of AIM2 inflammasome activation, which induced G2/M phase arrest and inhibited epithelial–mesenchymal transition (EMT) in NSCLC. Furthermore, the inhibitory effects of luteolin on NSCLC cells were abolished by the knockdown of AIM2. On the contrary, the antitumor effects of luteolin could be notably reversed by the overexpression of AIM2. In addition, luteolin reduced poly(dA:dT)-induced caspase-1 activation and IL-1β cleavage in NSCLC cells. These findings suggested that AIM2 was essential to luteolin-mediated antitumor effects. The antitumor effects of luteolin, which were closely associated with AIM2, were also confirmed in the A549 and H460 xenograft mouse models. Collectively, our study displayed that the antitumor effects of luteolin on NSCLC were AIM2 dependent and the downregulation of AIM2 might be an effective way for NSCLC treatment.
Decoherence of black hole superpositions by Hawking radiation
Authors: Arrasmith Andrew , Albrecht Andreas , Zurek Wojciech H. 
Published: March 2019

AbstractAn environment interacting with a system acquires information about it, e.g. about its location. The resulting decoherence is thought to be responsible for the emergence of the classical realm of our Universe out of the quantum substrate. However, this view of the emergence of the classical is sometimes dismissed as a consequence of insufficient isolation and, hence, as non-fundamental. In contrast to many other systems, a black hole can never be isolated from its Hawking radiation which carries information about its location, making this lack of isolation fundamental. Here we consider the decoherence of a “black hole Schrödinger cat”—a non-local superposition of a Schwarzschild black hole in two distinct locations—due to its Hawking radiation. The resulting decoherence rate turns out to be given by a surprisingly simple equation. Moreover, and in contrast to known cases of decoherence, this rate does not involve Planck’s constant ħ.
Effects of 4-Hexylresorcinol on Protein Expressions in RAW 264.7 Cells as Determined by Immunoprecipitation High Performance Liquid Chromatography
Authors: Kim Min Keun , Yoon Cheol Soo , Kim Seong Gon , Park Young Wook , Lee Sang Shin , Lee Suk Keun 
Published: March 2019

Abstract4-Hexylresorcinol (4HR) is a small organic compound that is used as an additive antiseptic and antioxidant, but its molecular properties have not been clearly elucidated. The present study explored the cellular effects of 4HR on RAW 264.7 cells by immunoprecipitation high-performance liquid chromatography (IP-HPLC) using 216 antisera. 4HR-treated cells showed significant decreases in the expressions of proliferation-related proteins, cMyc/MAX/MAD network, p53/Rb/E2F and Wnt/β-catenin signalings, epigenetic modifications, and protein translation. Furthermore, 4HR suppressed the expressions of growth factors and proteins associated with RAS signaling, NFkB signaling, inflammation, and osteogenesis, but elevated the expressions of proteins associated with p53-mediated and FAS-mediated apoptosis, T-cell immunity, angiogenesis, antioxidant, and oncogenic signaling. In a 4HR adherence assay, TNFα, PKC, osteopontin, and GADD45 were strongly adherent to 4HR-coated beads, whereas IL-6, c-caspase 3, CDK4, and c-caspase 9 were not. Many 4HR adherent proteins were expressed at lower levels in 4HR treated RAW 264.7 cells than in non-treated controls, whereas 4HR non-adherent proteins were expressed at higher levels. These observations suggest 4HR affects the expressions of proteins in an adhesion-dependent manner and that its effects on proteins are characteristic and global in RAW 264.7 cells.
Legibility Assessment of Visual Word Form Symbols for Visual Tests
Authors: Tsai Li-Ting , Jang Yuh , Liao Kuo-Meng , Chen Chien-Chung 
Published: March 2019

AbstractFor a reliable visual test, it is important to evaluate the legibility of the symbols, which depends on several factors. Previous studies have compared the legibility of Latin optotypes. This study developed a visual function test based on identification visual capacity for a Chinese reading population. The legibility of word symbols was assessed with three methods: (1) Identification of the contrast thresholds of the character sets, (2) patterns of confusion matrices obtained from analysis of the frequency of incorrect stimulus/response pairs, and (3) pixel ratios of bitmap images of Chinese characters. Then characters of similar legibility in each character set were selected. The contrast thresholds of the final five character sets and the Tumbling E and Landolt C optotypes were evaluated. No significant differences in contrast threshold were found among the five selected character sets (p > 0.05), but the contrast thresholds were significantly higher than those of the E and C optotypes. Our results indicate that combining multiple methods to include the influences of the properties of visual stimuli would be useful in investigating the legibility of visual word symbols.
In vivo investigation of hyperpolarized [1,3-13C2]acetoacetate as a metabolic probe in normal brain and in glioma
Authors: Najac Chloé , Radoul Marina , Le Page Lydia M. , Batsios Georgios , Subramani Elavarasan , Viswanath Pavithra , Gillespie Anne Marie , Ronen Sabrina M. 
Published: March 2019

AbstractDysregulation in NAD+/NADH levels is associated with increased cell division and elevated levels of reactive oxygen species in rapidly proliferating cancer cells. Conversion of the ketone body acetoacetate (AcAc) to β-hydroxybutyrate (β-HB) by the mitochondrial enzyme β-hydroxybutyrate dehydrogenase (BDH) depends upon NADH availability. The β-HB-to-AcAc ratio is therefore expected to reflect mitochondrial redox. Previous studies reported the potential of hyperpolarized 13C-AcAc to monitor mitochondrial redox in cells, perfused organs and in vivo. However, the ability of hyperpolarized 13C-AcAc to cross the blood brain barrier (BBB) and its potential to monitor brain metabolism remained unknown. Our goal was to assess the value of hyperpolarized [1,3-13C2]AcAc in healthy and tumor-bearing mice in vivo. Following hyperpolarized [1,3-13C2]AcAc injection, production of [1,3-13C2]β-HB was detected in normal and tumor-bearing mice. Significantly higher levels of [1-13C]AcAc and lower [1-13C]β-HB-to-[1-13C]AcAc ratios were observed in tumor-bearing mice. These results were consistent with decreased BDH activity in tumors and associated with increased total cellular NAD+/NADH. Our study confirmed that AcAc crosses the BBB and can be used for monitoring metabolism in the brain. It highlights the potential of AcAc for future clinical translation and its potential utility for monitoring metabolic changes associated with glioma, and other neurological disorders.
Bimodal Incoherent Digital Holography for Both Three-Dimensional Imaging and Quasi-Infinite–Depth-of-Field Imaging
Authors: Nobukawa Teruyoshi , Katano Yutaro , Muroi Tetsuhiko , Kinoshita Nobuhiro , Ishii Norihiko 
Published: March 2019

AbstractAlthough three-dimensional (3D) imaging and extended depth-of-field (DOF) imaging are completely opposite techniques, both provide much more information about 3D scenes and objects than does traditional two-dimensional imaging. Therefore, these imaging techniques strongly influence a wide variety of applications, such as broadcasting, entertainment, metrology, security and biology. In the present work, we derive a generalised theory involving incoherent digital holography to describe both 3D imaging and quasi-infinite–DOF (QIDOF) imaging, which allows us to comprehensively discuss the functions of each imaging technique. On the basis of this theory, we propose and develop a bimodal incoherent digital holography system that allows both 3D imaging and QIDOF imaging. The proposed system allows imaging objects using spatially incoherent light and reconstructing 3D images or QIDOF images solely by changing the phase pattern of a spatial light modulator and without requiring mechanical adjustments or any other modifications to the setup. As a proof-of-principle experiment, we evaluate the DOF and record holograms of a reflective object with the proposed system. The experimental results show that the generalised theory is effective; our demonstration platform provides the function of 3D and QIDOF imaging.
Pore-scale characteristics of multiphase flow in heterogeneous porous media using the lattice Boltzmann method
Authors: Bakhshian Sahar , Hosseini Seyyed A. , Shokri Nima 
Published: March 2019

AbstractThis study provides a pore-scale investigation of two-phase flow dynamics during primary drainage in a realistic heterogeneous rock sample. Using the lattice Boltzmann (LB) method, a series of three-dimensional (3D) immiscible displacement simulations are conducted and three typical flow patterns are identified and mapped on the capillary number (Ca)-viscosity ratio(M) phase diagram. We then investigate the effect of the viscosity ratio and capillary number on fluid saturation patterns and displacement stability in Tuscaloosa sandstone, which is taken from the Cranfield site. The dependence of the evolution of saturation, location of the displacement front, 3D displacement patterns and length of the center of mass of the invading fluid on the viscosity ratio and capillary number have been delineated. To gain a quantitative insight into the characteristics of the invasion morphology in 3D porous media, the fractal dimension Df of the non-wetting phase displacement patterns during drainage has been computed for various viscosity ratios and capillary numbers. The logarithmic dependence of Df on invading phase saturation appears to be the same for various capillary numbers and viscosity ratios and follows a universal relation.
Microbial Colonization Coordinates the Pathogenesis of a Klebsiella pneumoniae Infant Isolate
Authors: Pope Jillian L. , Yang Ye , Newsome Rachel C. , Sun Wei , Sun Xiaolun , Ukhanova Maria , Neu Josef , Issa Jean-Pierre , Mai Volker , Jobin Christian 
Published: March 2019

AbstractEnterobacteriaceae are among the first colonizers of neonate intestine. Members of this family, such as Escherichia and Klebsiella, are considered pathobionts and as such are capable of inducing local and systemic disease under specific colonization circumstances. Interplay between developing microbiota and pathogenic function of pathobionts are poorly understood. In this study, we investigate the functional interaction between various colonization patterns on an early colonizer, K. pneumoniae. K. pneumoniae 51-5 was isolated from stool of a healthy, premature infant, and found to contain the genotoxin island pks associated with development of colorectal cancer. Using intestinal epithelial cells, macrophages, and primary splenocytes, we demonstrate K. pneumoniae 51-5 upregulates expression of proinflammatory genes in vitro. Gnotobiotic experiments in Il10−/− mice demonstrate the neonate isolate induces intestinal inflammation in vivo, with increased expression of proinflammatory genes. Regulation of microbiota assembly revealed K. pneumoniae 51-5 accelerates onset of inflammation in Il10−/− mice, most significantly when microbiota is naturally acquired. Furthermore, K. pneumoniae 51-5 induces DNA damage and cell cycle arrest. Interestingly, K. pneumoniae 51-5 induced tumors in ApcMin/+; Il10−/− mice was not significantly affected by absence of colibactin activating enzyme, ClbP. These findings demonstrate pathogenicity of infant K. pneumoniae isolate is sensitive to microbial colonization status.
Use of vasopressor for dialysis-related hypotension is a risk factor for death in hemodialysis patients: Nationwide cohort study
Authors: Kanda Eiichiro , Tsuruta Yuki , Kikuchi Kan , Masakane Ikuto 
Published: March 2019

AbstractBecause hypotension during hemodialysis (HD) makes continuation of HD difficult and is associated with mortality, pressor approaches are necessary for patients with hypotension. However, the relationships between the pressor approaches and the risk of death have not been clarified yet. We analyzed data from a nationwide prospective cohort study of the Japanese Society for Dialysis Therapy Renal Data Registry (n = 29,309). The outcome was all-cause one-year death. The association between the use of pressor approaches and the outcome was examined using Cox proportional hazards models adjusted for baseline characteristics, propensity score matched analysis and Bayesian networks. The background features of the patients were as follows: male, 59.6%; average age, 64.5 ± 12.5 years; and patients with diabetes mellitus, 31.5%. The pressor group showed a higher risk of the outcome than the control group [adjusted hazard ratio (aHR) 1.33 (95% CI: 1.21, 1.47), p = 0.0001]. Propensity score matched analysis also showed that the matched-pressor group had a higher risk of the outcome than the matched-control group [aHR 1.30 (95% CI: 1.17, 1.45), p = 0.0001]. Moreover, the Bayesian network showed a direct causal relationship from the use of pressor approaches to the outcome. The use of oral vasopressors [aHR 1.20 (95% CI: 1.07, 1.35), p = 0.0018], intravenous injection of vasopressors [aHR 1.54 (95% CI: 1.32, 1.79), p = 0.0001] and normal saline [aHR 1.18 (95% CI: 1.05, 1.33), p = 0.0066] were associated with a high risk of the outcome. In conclusion, this study showed that the use of pressor approaches during HD may be an independent risk factor for death.
SOX6 blocks the proliferation of BCR-ABL1+ and JAK2V617F+ leukemic cells
Authors: Barbarani Gloria , Fugazza Cristina , Barabino Silvia M. L. , Ronchi Antonella E. 
Published: March 2019

AbstractSOX6 is a HMG-box transcription factor expressed in a wide range of tissues. Recent data show that SOX6 expression is altered in different cancers, in the majority of cases being downregulated. To date, no data are available about SOX6 role in hematological malignancies. Here we demonstrate that SOX6 overexpressing BCR-ABL1+ B-ALL cells are unable to promote leukemia in a mouse model. Starting from this observation, we extended our study to a panel of human leukemic cells carrying genetic lesions distinctive of different types of leukemias and myeloproliferative disorders (the BCR-ABL1 translocation and the JAK2V617F amino acid substitution) to dissect the cellular events induced by SOX6. The inhibition of proliferation is the invariant outcome of SOX6 overexpression but it is achieved via two different cellular responses: terminal differentiation in erythroid-biased cells, irrespectively of their mutation, and apoptosis in megakaryocytic-primed and lymphoid cells. Within this context, cells carrying the highest copy number of the JAK2V617F allele better counteract the SOX6-imposed growth arrest. The interrogation of the GEPIA (Gene Expression Profiling Interactive Analysis) human dataset reveals that SOX6 is downregulated in a cohort of AML patients, uncovering a wide anti-proliferative role of SOX6 in a variety of mutant backgrounds.
T cells in mesenteric and subcutaneous adipose tissue of Holstein-Friesian cows
Authors: Oliveira Bárbara M. , Rasteiro Ana M. , Correia Alexandra , Pinto Ana , Meireles Pedro , Ferreira Paula G. , Vilanova Manuel , Teixeira Luzia 
Published: March 2019

AbstractThe importance of immune cells present in the adipose tissue to metabolic homeostasis has been increasingly recognized. Nevertheless, in bovines few studies have so far addressed the immune cell populations resident in this tissue. Here we developed an eight-colour flow cytometry panel to address T cell populations present in bovine adipose tissue. Our results showed that γδ T cells, CD4+ and CD8+ CD3+ non-γδ T cells, as well as NK cells, are present in the mesenteric and subcutaneous adipose tissue of Holstein-Friesian cows. The frequency of both γδ T cells and CD8+ non-γδ T cells was found higher in mesenteric than in subcutaneous adipose tissue. The majority of T cells in adipose tissue presented a CD45RO+CD62L− phenotype, characteristic of effector memory cells, and the frequency of these cellular populations was higher than in the blood. The ratio of CD4+ T cells over CD8+ T cells was similar between subcutaneous and mesenteric adipose tissue but different from the one found in blood. Overall, our results highlight particular phenotypic characteristics of bovine adipose tissue T cell populations.
The Impact of Varying Cooling and Thawing Rates on the Quality of Cryopreserved Human Peripheral Blood T Cells
Authors: Baboo Jasmin , Kilbride Peter , Delahaye Mike , Milne Stuart , Fonseca Fernanda , Blanco Magdalena , Meneghel Julie , Nancekievill Alex , Gaddum Nick , Morris G. John 
Published: March 2019

AbstractFor the clinical delivery of immunotherapies it is anticipated that cells will be cryopreserved and shipped to the patient where they will be thawed and administered. An established view in cellular cryopreservation is that following freezing, cells must be warmed rapidly (≤5 minutes) in order to maintain high viability. In this study we examine the interaction between the rate of cooling and rate of warming on the viability, and function of T cells formulated in a conventional DMSO based cryoprotectant and processed in conventional cryovials. The data obtained show that provided the cooling rate is −1 °C min−1 or slower, there is effectively no impact of warming rate on viable cell number within the range of warming rates examined (1.6 °C min−1 to 113 °C min−1). It is only following a rapid rate of cooling (−10 °C min−1) that a reduction in viable cell number is observed following slow rates of warming (1.6 °C min−1 and 6.2 °C min−1), but not rapid rates of warming (113 °C min−1 and 45 °C min−1). Cryomicroscopy studies revealed that this loss of viability is correlated with changes in the ice crystal structure during warming. At high cooling rates (−10 °C min−1) the ice structure appeared highly amorphous, and when subsequently thawed at slow rates (6.2 °C min−1 and below) ice recrystallization was observed during thaw suggesting mechanical disruption of the frozen cells. This data provides a fascinating insight into the crystal structure dependent behaviour during phase change of frozen cell therapies and its effect on live cell suspensions. Furthermore, it provides an operating envelope for the cryopreservation of T cells as an emerging industry defines formulation volumes and cryocontainers for immunotherapy products.
Pathologist-level classification of histologic patterns on resected lung adenocarcinoma slides with deep neural networks
Authors: Wei Jason W. , Tafe Laura J. , Linnik Yevgeniy A. , Vaickus Louis J. , Tomita Naofumi , Hassanpour Saeed 
Published: March 2019

AbstractClassification of histologic patterns in lung adenocarcinoma is critical for determining tumor grade and treatment for patients. However, this task is often challenging due to the heterogeneous nature of lung adenocarcinoma and the subjective criteria for evaluation. In this study, we propose a deep learning model that automatically classifies the histologic patterns of lung adenocarcinoma on surgical resection slides. Our model uses a convolutional neural network to identify regions of neoplastic cells, then aggregates those classifications to infer predominant and minor histologic patterns for any given whole-slide image. We evaluated our model on an independent set of 143 whole-slide images. It achieved a kappa score of 0.525 and an agreement of 66.6% with three pathologists for classifying the predominant patterns, slightly higher than the inter-pathologist kappa score of 0.485 and agreement of 62.7% on this test set. All evaluation metrics for our model and the three pathologists were within 95% confidence intervals of agreement. If confirmed in clinical practice, our model can assist pathologists in improving classification of lung adenocarcinoma patterns by automatically pre-screening and highlighting cancerous regions prior to review. Our approach can be generalized to any whole-slide image classification task, and code is made publicly available at https://github.com/BMIRDS/deepslide.
Genomics-based diversity analysis of Vanilla species using a Vanilla planifolia draft genome and Genotyping-By-Sequencing
Authors: Hu Ying , Resende Marcio F. R. , Bombarely Aureliano , Brym Maria , Bassil Elias , Chambers Alan H. 
Published: March 2019

AbstractDemand for all-natural vanilla flavor is increasing, but its botanical source, Vanilla planifolia, faces critical challenges arising from a narrow germplasm base and supply limitations. Genomics tools are the key to overcoming these limitations by enabling advanced genetics and plant breeding for new cultivars with improved yield and quality. The objective of this work was to establish the genomic resources needed to facilitate analysis of diversity among Vanilla accessions and to provide a resource to analyze other Vanilla collections. A V. planifolia draft genome was assembled and used to identify 521,732 single nucleotide polymorphism (SNP) markers using Genotyping-By-Sequencing (GBS). The draft genome had a size of  2.20 Gb representing 97% of the estimated genome size. A filtered set of 5,082 SNPs was used to genotype a living collection of 112 Vanilla accessions from 23 species including native Florida species. Principal component analysis of the genetic distances, population structure, and the maternally inherited rbcL gene identified putative hybrids, misidentified accessions, significant diversity within V. planifolia, and evidence for 12 clusters that separate accessions by species. These results validate the efficiency of genomics-based tools to characterize and identify genetic diversity in Vanilla and provide a significant tool for genomics-assisted plant breeding.
Laboratory and in-situ investigations for trapping Pb and Ni with an unusual electrochemical device, the calcareous deposit in seawater
Authors: Carré Charlotte , Gunkel-Grillon Peggy , Serres Arnaud , Jeannin Marc , Sabot René , Quiniou Thomas 
Published: March 2019

AbstractIn seawater, the application of a cathodic current in a metallic structure induces the formation of a calcareous deposit formed by co-precipitation of CaCO3 and Mg(OH)2 on the metal surface. A previous study proved that this electrochemical technique is convincing as a remediation tool for dissolved nickel in seawater and that it is trapped as nickel hydroxide in the deposit. Here, the precipitation of a carbonate form with lead is studied. Pb2+ precipitation in calcareous deposit was investigated with a galvanized steel electrode by doping artificial seawater with PbCl2. Results show for the first time the presence of Pb incorporated in its carbonate form in the calcareous deposit. Trapped Pb content increased with initial Pb content in seawater. Simultaneous doping with Ni and Pb revealed that Ni trapping was favoured by higher current densities while Pb trapping was favoured by lower current densities. Finally, preliminary in situ experiments were performed in an industrial bay and validated the incorporation in real conditions of contaminants by precipitation with the calcareous deposit The present work demonstrates that co-precipitation of contaminants under their hydroxide or carbonate form in a calcareous deposit is a promising clean-up device for remediation of contaminated seawater.