bluuebunny/crossref_metadata_2025_split huggingface dataset:
Electronic Audit and Feedback With Positive Rewards Improve Anesthesia Provider Compliance With a Barcode-Based Drug Safety System
Authors: Bowdle T. Andrew , Jelacic Srdjan , Nair Bala , Zucker Frank , Bussey Logan S. , Togashi Kei , Yang Jen-Ting , Lang John 
Published: October 2018

BACKGROUND:
We implemented a previously described barcode-based drug safety system in all of our anesthetizing locations. Providers were instructed to scan the barcode on syringes using our Anesthesia Information Management System before drug administration, but the rate of provider adherence was low. We studied an implementation intervention intended to increase the rate of scanning.

METHODS:
Using our Anesthesia Information Management System and Smart Anesthesia Manager software, we quantified syringe drug administrations by anesthesia providers with and without barcode scanning. We use an anesthesia team model in which an attending anesthesiologist is paired with a certified registered nurse anesthetist (CRNA) or a resident. Our system identified the pair of providers associated with a particular drug administration, but did not distinguish which providers actually administered the drug. Therefore, the rate of barcode scanning for a particular case was assigned to both providers equally. A baseline rate of scanning was established over a period of 17 months. An audit and feedback intervention was then performed that consisted of monthly performance reports sent by email to individual providers along with coffee gift card awards for top performers. The coffee gift cards were awarded in only the first 2 months of the intervention, while the email performance reports continued on a monthly basis. The coffee card awards were made public. The monthly emails reported the individual provider’s rank order of performance relative to other providers, but was otherwise anonymous. The baseline rate of scanning was compared to the rate of scanning after the intervention for a period of 7 months.

RESULTS:
From November 2014 to March 2017, we accumulated 60,197 cases performed by 88 attending anesthesiologists, 65 CRNAs, and 148 residents. The total number of syringe drug administrations was 653,355. Average scanning performance improved from 8.7% of syringe barcodes scanned during the baseline period from November 2014 to February 2016 to 64.4% scanned during the period September 2016 to March 2017 (P < .001). Variation in performance among individuals was marked, ranging from 0% to 100% of syringes scanned. The performance of some individuals showed marked oscillation over time. There was greater variation in performance attributable to residents than in performance attributable to CRNAs.

CONCLUSIONS:
Feedback of individual provider performance data from the anesthesia information system to providers can be used in conjunction with other measures to improve performance. Despite improved average performance, there was marked variation in performance between individuals, and some individuals had marked oscillation of their performance over time.
Endocytic trafficking factor VPS45 is essential for spatial regulation of lens fiber differentiation in zebrafish
Authors: Mochizuki Toshiaki , Kojima Yutaka , Nishiwaki Yuko , Harakuni Tetsuya , Masai Ichiro 
Published: October 2018

ABSTRACT
In vertebrate lens, lens epithelial cells cover the anterior half of the lens fiber core. Lens epithelial cells proliferate, move posteriorly and start to differentiate into lens fiber cells at the lens equator. Although FGF signaling promotes this equatorial commencement of lens fiber differentiation, the underlying mechanism is not fully understood. Here, we show that lens epithelial cells abnormally enter lens fiber differentiation without passing through the equator in zebrafish vps45 mutants. VPS45 belongs to the Sec1/Munc18-like protein family and promotes endosome trafficking, which differentially modulates signal transduction. Ectopic lens fiber differentiation in vps45 mutants does not depend on FGF, but is mediated through activation of TGFβ signaling and inhibition of canonical Wnt signaling. Thus, VPS45 normally suppresses lens fiber differentiation in the anterior region of lens epithelium by modulating TGFβ and canonical Wnt signaling pathways. These data indicate a novel role of endosome trafficking to ensure equator-dependent commencement of lens fiber differentiation.
X-ray Absorption Spectroscopy on Atomically Precise Metal Clusters
Authors: Yamazoe Seiji , Tsukuda Tatsuya 
Published: October 2018

Abstract
Metal clusters show novel and size-specific properties due to unique geometric and quantized electronic structures. State-of-the art synthetic methods allow us to control with atomic precision the size and compositions of clusters stabilized with polymers, protected by ligands, and immobilized on supports. The geometric structure is key information for understanding the origin of the specific and novel properties and for rationally designing their functions. Single-crystal X-ray diffraction analysis provides direct and atomic-level structural information on ligand-protected metal clusters that can be crystallized, but cannot be applied to polymer-stabilized and supported clusters even though their size and composition are precisely defined. X-ray absorption spectroscopy (XAS) is a versatile tool for determining the local structure and electronic state of a specific element within the clusters regardless of their environment. In addition to static structures, dynamic changes in electronic and geometric structures can be probed by a time-resolved measurement. Simultaneous measurement of XAS with other spectroscopies provides further insight into the reaction mechanism. This article summarizes our XAS studies on the size and atomic packing of metal clusters, location of dopant in the clusters, interfacial structures between the clusters and the surroundings, thermal properties of the clusters, and structural and electronic dynamics during the reactions.
Do funding applications where peer reviewers disagree have higher citations? A cross-sectional study.
Authors: Barnett Adrian G , Glisson Scott R. , Gallo Stephen 
Published: October 2018

Background: Decisions about which applications to fund are generally based on the mean scores of a panel of peer reviewers. As well as the mean, a large disagreement between peer reviewers may also be worth considering, as it may indicate a high-risk application with a high return. Methods: We examined the peer reviewers' scores for 227 funded applications submitted to the American Institute of Biological Sciences between 1999 and 2006. We examined the mean score and two measures of reviewer disagreement: the standard deviation and range. The outcome variable was the relative citation ratio, which is the number of citations from all publications associated with the application, standardised by field and publication year. Results: There was a clear increase in relative citations for applications with a better mean. There was no association between relative citations and either of the two measures of disagreement. Conclusions: We found no evidence that reviewer disagreement was able to identify applications with a higher than average return. However, this is the first study to empirically examine this association, and it would be useful to examine whether reviewer disagreement is associated with research impact in other funding schemes and in larger sample sizes.
PL-002 AGTR1 polymorphism is associated with elite endurance athletes: A functional study
Authors: Yang Xiaolin , Hu Yang , Li Yanchun , Mei Tao , Gong Lijing 
Published: October 2018

Objective To explore the association between the polymorphism of angiotensin II receptor type 1 gene(AGTR1) and elite endurance athlete performance and the mechanism of how the polymorphism works.
Methods (1) Polymorphism of AGTR1 rs5182 between 122 elite Chinese endurance athletes and 222 controls were analyzed by MALDI-TOF-MS. (2) Aerobic capacity of 79 elite Chinese endurance athletes such as VC/FEV1/MVV/ VO2AT/ HRAT/ VAT/ VO2max/ HRmax/ VVO2max were measured and association between rs5182 polymorphism and the performance was analyzed. (3) PcDNA3.1-AGTR1 -T and pcDNA3.1-AGTR1-C plasmid were build and the plasmids was transfected into mammalian 293T cells. mRNA levels were detected after 48 hours. Statistical analysis was performed using SPSS software version 15.0. Values of P < 0.05 were considered statistically significant. Continuous data were expressed as mean ± SD, while categorical data were expressed as frequencies. Genotype distribution and allele frequencies between athletes and control subjects were compared using χ2 tests. Aerobic performance data was analyzed with One-Way ANOVA if it conformed to normal distribution and homogeneity of variance otherwise Non-parametric test of independent sample was used.
Results (1) Genotype frequencies of AGTR1 rs5182 are significant differences between the athletes and control subjects (p = 0.040), the Word-Class athletes and control subjects (p = 0.018), 5km athletes and control subjects (p =0.015), 10km athletes and control subjects (p = 0.026), male athletes and male controls(p=0.045). (2) Association is found between Genotype distribution and MV(L/min) though others not (Genotype: MV; CC: 122.514±6.767; CT:117.187±17.961; TT:119.688±20.226, p=0.047). (3) Transiently transfectedpcDNA3.1-AGTR1-T and pcDNA3.1-AGTR1-C plasmids into 293T cells successfully. The differences of mRNA levels between the groups were not significant (p = 0.991).
Conclusions AGTR1 gene rs5182 could be a candidate genetic mark of selection elite endurance athletes in Han Population from Northern China, but this polymorphism does not affect AT1R protein function through changing its mRNA level.
PL - 023 Effects of Oleanolic Acid on Omega-3 and 6 Fatty Acids Metabolism in Rats fed with High Fructose Diet
Authors: Fuku Sandile 
Published: October 2018

Objective The present study investigated the effect of OA on metabolism of omega 3 and 6 together with genes associated with lipid metabolism in high fructose diet fed Sprague Dawley rats.
Methods Real Time PCR qPCR was conducted to evaluate gene expressions levels.
Gas Chromatography Mass Spectrometry (GS-MS): Lipid metabolism metabolites were assesed.
Measurement of gene expression using qPCR analysis: Expressions levels of glucose transport and lipid metabolism genes were assessed.
Measurements of inflammatory markers concentration in Blood plasma:TNF-α and adiponectin concentrations were determined.
Results It was found that high fructose diet decreased gene ex pression levels of NRF-1 and GLUT-4 genes, however administration of OA increased expression levels of these genes. Furthermore, adiponectin concentration was lowered in HFD group however this was reversed when HFD was accompanied by OA administration. This increase in concentration of the anti-inflammatory molecule followed the increase in adiponectin receptor, AdipoR1 gene expression in skeletal muscle tissue. The results on lipid metabolism metabolites were consistent the observed molecular events, the anti-inflammatory signaling molecules (EPA and DHA) were five times higher in HF+OA than they were in HFD. High FAS,ACC-2 gene expression levels in HFD group, low 2 expression levels these genes together with increased CPT-1 in OA treated groups coincided with increased levels of arachidonic acid and linoleic acid levels in HFD, threefold increase compared to the control, and decreased levels in OA treated rats.
Conclusions Supplementation of diet with oleanolic acid produces DHA and EPA, thus modulating anti-inflammatory response and modulating effects of exercise in reversing diabetes. We provided evidence to effect that diet has a therapeutic potential in managing metabolic syndrome related diseases.
Effects of HIITand MICT for 10 weeks on myocardial AMPK and PGC-1α in rats
Authors: Su Hao , Jiang Zhongye , Cao Guohuan , Wen Tianhao , Shao Jia , Li Liang 
Published: October 2018

Objective: The improvement of cardiorespiratory fitness (CRF) is known as an effective strategy for prevention cardiovascular risk. Myocardial aerobic oxidation which control by the signal way of adenosine monophosphate -activated protein kinase (AMPK)- peroxisome proliferators γ activated receptor coativator-1-α (PGC-1α) is the key for CRF. Previous studies only discuss the effect of the Moderate-Intensity Continuous Training (MICT) and High Intensive Interval Training (HIIT) on the signal way of AMPK- PGC-1α in skeletal muscle but not in the myocardium. The aim of this study was to compare the effects of 10 weeks HIIT and MICT on the expression of AMPK and PGC-1α in the myocardium of wistar male rats.
Methods: Wistar male rats (n=30) aged 6 weeks were randomly divided into HIIT or MICT or control (CON) group. The training groups ran on a treadmill 5 days/week for 10 weeks. HIIT group ran six times 3 minutes (0° slope) 90% of Vmax separated by 3 minutes 50% of Vmax and MICT group ran for 50min (0° slope) at 60–70% of maximal speed (Vmax). The expression of AMPK and PGC-1α were assessed by Western Blotting.
Results: After 10 weeks training, HIIT and MICT both increased the AMPK and PGC-1α expression compared with the CON group. Compared with the MICT group, the expression of AMPK and PGC-1α were significantly higher than the HIIT group (p<0.05). AMPK in MICT group were significant increased 1.16 times, and in HIIT group were significant increased 1.28 times to CON (P<0.05). PGC-1α level of HIIT was significant increased to 1.32 times to CON and also significant increased to 1.15 times to Group M (P<0.05); PGC-1α level of MICT was significant increased to 1.15 times to CON.
Conclusion：HIIT seems to improve myocardial AMPK and PGC-1α more efficiently than MICT in rats after 10 weeks training.
OR-024 Changes of mitochondrial autophagy - related genes and autophagosome after skeletal muscle blunt trauma
Authors: Dong Guijun , Bi Jun , Li Kefeng Li , Dai Lulu , Zong Wenhao 
Published: October 2018

Objective Objective: To study the changes of mitochondrial autophagy-related genes and autophagosome after skeletal muscle blunt trauma, to reveal the changes of mitochondrial adaptive repair process after skeletal muscle blunt trauma, and to elucidate the mechanism of blunt trauma repair process.
Methods Methods: Sixty - four male Wistar rats were randomly divided into control group and blunt trauma group三 (divided into 12h group, 2d group, 5d group, 7d group, 10d group, 15d group and 30d group) according to the time of extraction. The expression of HIF-1α, AMPKα2, BNIP3 and NIX protein in skeletal muscle hypoxia and autophagy-related factors were measured by Western-Blot. QRT-PCR was performed to analyze the expression levels of HIF-1α, AMPKα2, BNIP3 and NIX. The ultrastructure and autophagic formation at different time points were observed by transmission electron microscopy (TEM).
Results Results: The expression of HIF-1α and AMPKα2 protein reached the peak at 12h and 2d, and the expression of HIF-1α was significantly higher than that of the control group (P <0.05). The expression of AMPKα2 was significantly higher at 5 days after injury (P <0.05), and reached the normal level at 10 days. BNIP3 began to decline after 5 days, but still higher than normal at 10 days after treatment. NIX expression peak appeared at 12h and 2d after injury, with high-express to 7d. The expression of HIF-1α and AMPKα2 mRNA was significantly higher than that of the control group (P <0.01), but decreased until 5d (P <0.05), then decreased to normal level. The mRNA expression of BNIP3 and NIX was basically the same as their protein performance. A number of autophagosomes were observed at 12 h after injury, and the number of autophagosomes increased gradually at 2-7 d. After 10 days, the number of autophagosomes decreased compared with that of 12 h-7 d after blunt. And after 15 days, the number of autophagosites decreased gradually.
Conclusions Conclusion: The changes of early stage metabolic regulator AMPKα2 and hypoxia-sensitive factor HIF-1αafter skeletal muscle blunt trauma indicated that an energy crisis occurred in the skeletal muscle after injury, and the hypoxic environment was formed. The mitochondrial autophagy, the expression of BNIP3 and NIX showed that mitochondrial autophagy was activated and hypoxia induced mitochondrial autophagy at early skeletal muscle contusion peroid. Hypoxia-induced mitochondrial autophagy could remove the damaged mitochondria, maintain mitochondrial quality and provide raw materials for new mitochondria generation, facilitate the rapid recovery of damaged skeletal muscle, which may be a compensatory mechanism of the body response to injury.
OR-047 Mechanical stretch activates glycometabolism-related enzyme through estrogen in C2C12 myoblasts
Authors: Feng Yu , Shi RengFei , Lv JianQiang 
Published: October 2018

Objective Exercise is involved with some Metabolic diseases, moderate exercise may improve glycometabolism and type 2 diabetes mellitus in menopausal female. Previous study showed that exercise increased the level of muscular estrogen in Ovariectomized rats, improved muscle mass and glycometabolism, it provided a reference to relief type 2 diabetes mellitus symptom. Until now, the effect of estrogen induced by exercise on muscular glycometabolism is not clear, the present study was designed to explore the effect of estrogen induced by mechanical stretch on glycometabolism in mouse C2C12 myoblasts.
Methods The mouse C2C12 myoblasts in vitro were plated at BioFlex Culture Plate, and assigned randomly to the control group(C), stretch group(S), SA group. SA group was cultured in growth medium with 400μg/ml anastrozole(aromatase inhibitor), other groups were cultured in GM with DMSO for 36h, and then S, SA groups were stretched by Flexcell FX-5000TM system (magnitude 15%, frequency 1Hz, duration 6hours), Cellular proteins were extracted after 24h of stretch, ELISA assay was used to detect estradiol levels, we detected the expression of HK, PI3K, the ratio of p-AKT and AKT, GLUT4 protein level by Western blotting.
Results Compared with the control group, a higher estradiol level was detected in stretch group(P<0.05), and the protein expression of HK, PI3K, the ratio of p-AKT and AKT, GLUT4 is higher(P<0.05) after stretching. The estradiol level and protein expression is lower in SAF group as compare to the stretch group(P<0.05). while there was no significant difference in estradiol level and protein expression between SF group and SAF group(P>0.05).
Conclusions Estrogen induced by mechanical stretch can improve glycometabolism-related enzyme and protein expression of mouse C2C12 myoblasts.
PO-046 Gene expression profiling of peripheral blood mononuclear cells in young male trampoline athletes
Authors: Gao Li , Yang Yong Jie , Li En Qi , Mao Jia Ning 
Published: October 2018

Objective Evidence indicates that physical activity influence bone health. However, the molecular mechanisms mediating the beneficial adaptations to exercise are not well understood. The purpose of this study was to examine the differentially expressed genes in PBMC between athletes and healthy controls, and to analyze the important functional genes and signal pathways that cause increased bone mineral density in athletes, in order to further reveal the molecular mechanisms of exercise promoting bone health.
Methods Five professional trampoline athletes and five age-matched untrained college students participated in this study. Used the human expression Microarray V4.0 expression profiling chip to detect differentially expressed genes in the two groups, and performed KEGG Pathway analysis and application of STRING database to construct protein interaction Network; Real-Time PCR technology was used to verify the expression of some differential genes.
Results Compared with healthy controls, there were significant improvement in lumbar spine bone mineral density, and 236 up-regulated as well as 265 down-regulated in serum samples of athletes. The differentially expressed genes involved 28 signal pathways, such as cell adhesion molecules. Protein interaction network showed that MYC was at the core node position. Real-time PCR results showed that the expression levels of CD40 and ITGα6 genes in the athletes were up-regulated compared with the healthy controls, the detection results were consistent with that of the gene chip.
Conclusions The findings highlight that long-term high-intensity trampoline training could induce transcriptional changes in PBMC of the athletes. These data suggest that gene expression fingerprints can serve as a powerful research tool to design novel strategies for monitoring exercise. The findings of the study also provide support for the notion that PBMC could be used as a substitute to study exercise training that affects bone health.
PO-079 Effect of Aerobic Exercise on the expression of VEGF in hippocampus and the Spatial Learning and Memorizing Abilities in CUMS-induced depressive Rats
Authors: Jin Yu , Yuan Qiongjia 
Published: October 2018

Objective Through the 4 weeks aerobic exercise intervention in rats with CUMS (chronic unpredictable mild stress) to build depression model of rats, and to explore the effects of aerobic exercise intervention on hippocampal VEGF expression and spatial learning and memorizing ability in depressive model rats.
Methods 30 adult 3-month-old Sprague-Dawley rats weighing 300 ± 20 g were used. After adaptive feeding for 1 week, they were randomly divided into 3 groups: control group (C), the model group (M), and exercise group (E). M and E groups were subjected to CUMS stimulation for 4 weeks and/or aerobic exercise for 4 weeks according to different modeling procedures. After exercise or CUMS, the behavioral index was tested by sucrose preference test (SPT) and Morris water maze (MWZ). The 5-HT expression of whole brain was detected by ELISA. And Real-time PCR, Western Blotting, HE staining and immunofluorescence method test the expression of VEGF and morphological structure to change in hippocampus.
Results Compared with the C group, the sucrose intake and the percentage of syrup preference were significantly decreased in the CUMS-induced depression rats, the 5-HT depression in the whole brain was significantly decreased, the hippocampal neurons were disorderly arranged and the number was less, and the hippocampal VEGF gene and protein were significantly decreased. While aerobic exercise can significantly improve the depression-like behavior, learning and memorizing ability of rats with depression, increase the expression of 5-HT in whole brain, and the hippocampal neurons are arranged neatly, clearly and in large quantities, and increase the gene and protein expression of VEGF in hippocampus.
Conclusions Four weeks aerobic exercise intervention can significantly up-regulate the expression of VEGF in hippocampus and improve learning and memorizing ability and depressive symptoms. It was suggested that the increased expression of VEGF in the hippocampus may be one of the neurobiological mechanisms in depression and spatial learning and memorizing ability.