bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of good and poor mobilizers on hematopoietic progenitor cell collection efficiency and product quality
Authors: Azzouqa Abdel‐Ghani M. , Jouni Kinda , Roy Vivek , Zubair Abba C. 
Published: November 2018

AbstractBackgroundMobilization regimen choice is a significant contributing factor for successful hematopoietic progenitor cell (HPC) collection by leukocytapheresis and reaching the target CD34+ cell dose. How mobilization regimen affects collection efficiency and the quality of products collected using the Spectra Optia apheresis instrument is not fully known.MethodsWe evaluated the impact of granulocyte‐colony stimulating factor (GCSF) and GCSF/plerixafor mobilization regimens on CE and product composition. We studied 373 leukocytapheresis HPC collections for 147 autologous transplants from January 1, 2010 to December 31, 2014. Patients were categorized in two groups; good mobilizers, mobilized with GCSF only (GM) and poor mobilizers, mobilized with GCSF and Plerixafor (PM).ResultsOverall, compared with PM group, total nucleated cell (TNC) yield was significantly lower in GM group (P = <.001). In contrast, median percent mononuclear cell (MNC) collected from GM (86.5%) was significantly higher than products collected from PM group (79.5%; P < .001). Compared with GM group, CD34+ cell CE was about 10% lower in PM group (P < .008). In addition, daily CD34+ cell/Kg yield was significantly higher in GM (2.08 × 10/Kg) compared with PM group (1.64 x 10/Kg, P = .019). Overall, the median number of collections per patient was two for GM and three for PM (P = .004).ConclusionProducts collected from PM group contained higher TNC content relative to GM group but had lower MNC enrichment, CD34+ cell CE and daily CD34+ cell yield per Kg.
Cepharanthine suppresses osteoclast formation by modulating the nuclear factor‐κB and nuclear factor of activated T‐cell signaling pathways
Authors: Lin Xixi , Song Fangming , Zhou Lin , Wang Ziyi , Wei Chengming , Xu Jiake , Zhao Jinmin , Liu Qian 
Published: November 2018

AbstractThe increased activation of osteoclasts is the major manifestation of several lytic bone diseases, including osteoporosis, rheumatoid arthritis, aseptic loosening of orthopedic implants, Paget disease and malignant bone diseases. One important bone‐protective therapy in these diseases focuses on the inhibition of osteoclast differentiation and resorptive function. Given that the deleterious side‐effects of currently available drugs, it is beneficial to search for effective and safe medications from natural compounds. Cepharanthine (CEP) is a compound extracted from Stephania japonica and has been found to have antioxidant and anti‐inflammatory effects. In this study, we found that CEP inhibited receptor activator of nuclear factor‐κB (NF‐κB) ligand (RANKL)‐induced osteoclast formation and bone‐resorbing activities using osteoclastogenesis and bone resorption assay. By polymerase chain reaction, we also found that CEP inhibited the expression of osteoclast‐differentiation marker genes including Ctsk, Calcr, Atp6v0d2, Mmp9 and Nfatc1. Mechanistic analyses including Western blot and luciferase reporter assay revealed that CEP inhibited RANKL‐induced activation of NF‐κB and nuclear factor of activated T‐cell, which are essential for the formation of osteoclast. Collectively, these data suggested that CEP can potentially be used as an alternative therapy for preventing or treating osteolytic diseases.
circRNAs (hsa_circ_00156, hsa_circ _000224, and hsa_circ _000520) are novel potential biomarkers in hepatocellular carcinoma
Authors: Matboli Marwa , Shafei Ayman E , Ali Mahmoud A , Ashry Ahmed M , Kamal Kamal M , Agag Mohammed Ali , Reda Ibrahim , Tash Eman Fathy , Ali Marwa 
Published: November 2018

AbstractCircular RNAs (circRNAs) are a newly validated type of noncoding RNAs recently found to be deregulated in several human cancers. More accurate and specific noninvasive biomarkers are strongly needed for better diagnosis and prognosis of hepatocellular carcinoma (HCC). We performed a bioinformatics analysis to retrieve a novel panel of circRNAs potentially relevant to HCC. We examined their expression in the sera of 68 patients with HCC, 60 patients with chronic hepatitis C, and 36 healthy controls using quantitative polymerase chain reaction. We examined the performance characteristics of the selected circRNA biomarker panel in comparison with alpha‐fetoprotein (AFP). In addition, we performed a survival analysis to correlate between their expression levels and patient survival. The circRNAs hsa_circ _00224 and hsa_circ _00520 showed a strong biomarker potential with relatively high sensitivities and specificities compared with AFP. The combined panel including the three circRNAs showed superior performance characteristics relative to those of AFP. The median follow‐up period was 26 months. hsa_circ_00520 expression has been shown to be associated with relapse‐free survival (P < 0.005). circRNAs hsa_circ_00156, hsa_circ_000224, and hsa_circ_000520 are novel potential biomarkers of high sensitivity and specificity, which could potentially be used in the diagnosis of HCC.
MKL1/miR34a/FOXP3 axis regulates cell proliferation in gastric cancer
Authors: Li Jia‐Peng , Liao Xing‐Hua , Xiang Yuan , Yao Ao , Fan Li‐Juan , Li Hui , Zhang Zi‐Jian , Huang Feng , Dai Zhou‐Tong , Zhang Tong‐Cun 
Published: November 2018

AbstractMegakaryoblastic leukemia 1 (MKL1) was closely related to the pathogenesis of various human malignant cancers. MiR34a was reported to be closely related to cancer cell proliferation. Forkhead box protein 3 (FOXP3) was a transcription factor that played a different role in different cancer types. CDK6 was involved in cell cycle progression and was upregulated in several types of cancers. The present study investigated the effects of MKL1/miR34a/FOXP3 axis on cell proliferation in MGC803 gastric cancer cells. Our results demonstrated that overexpression of MKL1 promoted proliferation of MGC80‐3 cells, MKL1 directly binding to the promoter of CDK6 to increase its expression. Knockdown of FOXP3 promoted proliferation of MGC80‐3 cells and MKL1 inhibited the expression of FOXP3 via miR‐34a. The finding can contribute to elucidating the regulatory mechanism involved in the cell cycle progression of gastric cancer cells and may aid in screening potential gene targets for the biological therapy of gastric cancer.
Tumor‐suppressive microRNA‐10a inhibits cell proliferation and metastasis by targeting Tiam1 in esophageal squamous cell carcinoma
Authors: Liu Yatian , Wang Xiaojun , Jiang Xuesong , Yan Pengwei , Zhan Liangliang , Zhu Huanfeng , Wang Tingting , Wen Jing 
Published: November 2018

AbstractAberrant microRNAs (miRNAs) expressions could contribute to the progression of numerous cancers, including esophageal squamous cell carcinoma, while miR‐10a participates in multiple biological processes on cancers. However, the molecular mechanism of miR‐10a in esophageal squamous cell carcinoma (ESCC) has not been investigated. Herein, miR‐10a was significantly reduced in ESCC clinical tissues and ESCC cell lines (EC109 and TE‐3). In addition, immunohistochemistry indicated that the expressions of α‐SMA, Ki‐67, and PCNA in tumor tissues were higher than that of controls. In vitro, overexpression of miR‐10a dramatically suppressed cell proliferation and enhanced cell apoptosis, while the decrease of miR‐10a expressed the opposite outcome. Specially, overexpression of miR‐10a caused a G0/G1 peak accumulation. Moreover, miR‐10a also negatively regulated ESCC cell migration and invasion. Furthermore, targetscan bioinformatics predictions and the dual‐luciferase assay confirmed that Tiam1 was a direct target gene of miR‐10a. The statistical analysis showed Tiam1 was negatively in correlation with miR‐10a in ESCC patient samples. And silencing Tiam1 could lead to a decline on cell growth, invasion, and migration in ESCC cell lines, while it could enhance cell apoptosis and cause a G0/G1 peak accumulation. In vivo, it revealed that miR‐10a notably decreased the tumor growth and metastasis in xenograft model and pulmonary metastasis model. And it showed a lower expressions of Tiam1 in the miR‐10a mimics group by immunohistochemistry. Taken together the results, they indicated that miR‐10a might function as a novel tumor suppressor in vitro and in vivo via targeting Tiam1, suggesting miR‐10a to be a candidate biomarker for the ESCC therapy.
Synergistic effect between H2O and SO2 on mercury removal by activated carbon in O2/CO2 conditions
Authors: Wang Hui , Shen Chang , Duan Yufeng , Ying Zhanfeng , Li Ya‐Ning 
Published: November 2018

AbstractBACKGROUNDThis work evaluated the mechanism of H2O on mercury oxidation and adsorption by activated carbon in simulated flue gas in a fixed‐bed reactor. The effect of the addition of SO2 on the mercury removal was also discussed. Mercury desorption tests were conducted in a setup for classifying mercury forms adsorbed on activated carbon by the temperature‐programmed desorption (TPD) method.RESULTSThe results indicated that H2O could inhibit Hg0 removal through blocking the microporous channels of activated carbon. Additionally, the enriched H2O could provide the electrons to inhibit Hg0 oxidation. While medium H2O concentrations around 15% could dominate the competitive adsorption in O2/CO2, high and low H2O concentrations of 23% and 8%, respectivly, could not dominate the competition.CONCLUSIONWith the addition of SO2 in O2/CO2, Hg0 removal efficiency decreased with H2O increased from 8% to 23%. Furthermore, Hg0 adsorption proportion were all larger than 87% with the addition of SO2 with H2O in O2/CO2, which indicated that Hg0 adsorption was the main contributor to Hg0 removal performance. These results are useful for the control of mercury in O2/CO2 combustion technology. © 2018 Society of Chemical Industry
Synthesis and characterization of carbon microspheres from rubber wood by hydrothermal carbonization
Authors: Ahmed Khan Tanveer , Kim Hyun‐Joong , Gupta Arun , Jamari Saidatul S , Jose Rajan 
Published: November 2018

AbstractBACKGROUNDCarbon is the raw material for many commercial products; conventionally their production is from non‐renewable sources such as petroleum coke, pitch and coal. Recently carbon has been obtained from bioresources because of their renewability and high lignocellulosic content. This article details the synthesis of carbon microspheres from rubber wood, which is one of the largest commodity plants, via hydrothermal carbonization (hydrothermal rubber wood carbon; HTRW carbon) and evaluation of their characteristics.RESULTSTwo sets of carbon were synthesized: (i) in the first set, excess of water (20–40 × weight of biomass) was used in the hydrothermal process at 180–260 °C for 3–9 h; and (ii) in the second set, water ratio was 25–35 × weight of biomass and the hydrothermal carbonization (HTC) reaction temperature was fixed at 260 °C. The H/C and O/C ratios of starting rubber wood were ∼1.78 and ∼0.85, respectively, which upon processing through the first strategy resulted in H/C ∼0.78 and O/C ∼0.29; thereby suggesting increased condensation under HTC. On the other hand, the carbonization process was accelerated by water when the temperature was maintained at 260 °C; Fourier transform infrared (FTIR) studies show that this carbon has a different chemical structure from the starting rubber wood. Scanning electron microscopy (SEM) images showed that HTRW carbon was in the form of microspheres (size ∼1.5–5 µm).CONCLUSIONHTRW carbon with carbon content as high as 68% was developed from rubber wood biomass by hydrothermal processing of a mixture containing 35 times more water than the solid raw biomass at a temperature of 260 °C for 7 h. © 2018 Society of Chemical Industry
Characterization of transport through polymers for fracking fluid treatment and organic acid concentration in extractive membrane bioreactors
Authors: Mullins Nathan R , Daugulis Andrew J 
Published: November 2018

AbstractBACKGROUNDThe water‐intensive practice of hydraulic fracturing produces wastewater containing a variable matrix of organic and inorganic compounds, including ions and ionizable organic compounds. Extractive membrane bioreactors (EMBs) operating with tailored polymer membranes can selectively sequester and/or transport these solutes for biological treatment. Four grades of Hytrel™ tubing were compared for their suitability in EMB systems, based on the polymers' thermodynamic affinity for solutes and their ability to transport or speciate ionic wastewater constituents.RESULTSOf the four Hytrel™ grades compared, high water content types (30% and 54% equilibrated water content) facilitated the transport of ionic species through the tubing, with all monovalent species being transported through both high‐water content grades, and lack of transport through the low‐water content tubing (3% and 5%). Undissociated organic acids were transported through all tubing types and dissociated acids were able to permeate only high‐water content grades. Using this differential ability to transport/not transport an organic acid depending on its dissociation state, a low‐water content Hytrel™ grade of tubing was able to concentrate a dilute butyric acid solution by 220% after 48 h.CONCLUSIONThe use of polymeric tubing for EMB applications for the treatment of hydraulic fracturing wastewater requires knowledge of both solute affinity and water content for complex waste streams, both of which affect the capability to transport organic and ionic species. © 2018 Society of Chemical Industry
Strategies for achieving high‐level and stable production of toxic Streptomyces phospholipase D in Escherichia coli
Authors: Xiong Weide , Zeng Xianhai , Ho Shih‐Hsin , Ling Xueping , Shen Liang , Yao Chuanyi , Lu Yinghua 
Published: November 2018

AbstractBACKGROUNDPhospholipase D (PLD) has great potential in the pharmaceutical and food industries for manufacturing bioactive phospholipids. Overproduction of PLD in Escherichia coli is difficult due to its high cytotoxicity which causes plasmid instability, short‐term PLD synthesis, cell lysis and PLD leakage. To address this issue, integrated optimization of PLD production in upstream and downstream processing was performed.RESULTSEffective strategies in upstream processing include the use of plasmids containing the pSC101 par region in recA− E. coli, the use of moderately strong and tightly regulated promoter PBAD, optimization of codon usage and amino acid supplementation, maintaining the best cellular state by supplementing nutrition, and saturation induction at low temperature. Using these strategies, a large amount of PLD (1.2 × 105 U L−1, 81.5 mg L−1) was obtained in the batch culture and PLD synthesis lasted 9 h. Additionally, cell lysis did not occur –99% of the recombinant PLD remained in the periplasm. Ultimately, the PLD was purified 327‐fold by a four‐step downstream process comprising extraction with 10 mmol L−1 Ethylenediaminetetraacetic acid and 0.05% (w/v) sodium deoxycholate, ammonium sulfate precipitation, anion exchange and Ni2+‐affinity chromatography.CONCLUSIONThis study provides the basis for large‐scale production of PLD in E. coli. © 2018 Society of Chemical Industry
Comparison between autologous and homologous blood transfusions in liver resection for biliary tract cancer: a propensity score matching analysis
Authors: Onoe Shunsuke , Yokoyama Yukihiro , Ebata Tomoki , Igami Tsuyoshi , Mizuno Takashi , Yamaguchi Junpei , Watanabe Nobuyuki , Nagino Masato 
Published: November 2018

AbstractBackgroundIt remains unclear whether preoperative blood donation is truly beneficial in liver surgery. The aim of this study was to compare surgical outcomes between patients receiving autologous and homologous transfusions during liver resection for biliary tract cancer (BTC).MethodsPatients who underwent hepatectomy for BTC were retrospectively reviewed (2006–2017). Patients who deposited autologous blood and underwent resection without homologous blood transfusion intraoperatively (Autologous group) were compared with non‐depositing patients who required homologous transfusion during hepatectomy (Homologous group). Propensity score matching analyses were performed to adjust the data for the baseline characteristics of both groups.ResultsDuring the study period, 359 patients were included in the Autologous group, and 105 patients were in the Homologous group. The postoperative maximum total bilirubin (T‐Bil) levels and the incidence of postoperative liver failure were significantly higher in the Homologous group than in the Autologous group. After propensity score matching, postoperative maximum T‐Bil levels were significantly higher in the Homologous group, whereas the incidence of postoperative liver failure was comparable between the two groups; between‐group differences were not observed for the remaining major complications, hospital stays and mortality.ConclusionAlthough autologous blood transfusion may minimize postoperative hyperbilirubinemia, it may not decrease the risk for mortality or morbidities following hepatectomy for BTC.
Natural Disaster Damages and Their Link to Coping Strategy Choices: Field Survey Findings from Post‐Earthquake Nepal
Authors: Rayamajhee Veeshan , Bohara Alok K. 
Published: November 2018

AbstractThe 7.8 magnitude earthquake that struck Nepal on 25 April 2015, was the worst natural disaster to hit Nepal since 1900. This article reports field survey findings on earthquake damages and ex‐post coping responses adopted by rural households in Sindhupalchowk, Nepal. Subsequently, we conduct empirical analyses to illustrate how earthquake damages influence ex‐post coping strategy choices and potentially limit paths to post‐disaster recovery. The goal of this empirical demonstration is to underscore the need to cater post‐disaster policies in developing countries in ways that expand households' post‐disaster coping strategy choices through market and non‐market mechanisms. © 2018 John Wiley & Sons, Ltd.
Radiolabeling, quality control, biodistribution, and imaging studies of 177Lu‐ibandronate
Authors: Xu Qin , Zhang Shumao , Zhao Yan , Feng Yue , Liu Lin , Cai Liang , Zhang Wei , Huang Zhanwen , Wei Hongyuan , Zhuo Liangang , Chen Yue 
Published: November 2018

The purposes of this study were as follows: (1) to radiolabel ibandronic acid (IBA, a third‐generation bisphosphonate) with 177Lu, investigating optimal labeling conditions, and (2) to analyze biodistribution and imaging properties of intravenous 177Lu‐ibandronate (177Lu‐IBA) administered in animals. 177Lu‐labeled methylene diphosphonate (177Lu‐MDP) served as a comparator agent. Differing proportions of IBA solution and 177LuCl3 solution were combined to determine an optimal ratio for radiolabeling purposes, varying pH, temperature, and time to establish ideal reactivity conditions. Radiochemical purity of the labeled compounds was then assessed by paper chromatography. In vitro and in vivo stabilities were also measured at specific time intervals. In Kunming mice, biodistributions of 177Lu‐IBA and 177Lu‐MDP and respective agent activities in various organs were monitored by gamma counter, and we performed single photon computed tomography/computed tomography (SPECT/CT) imaging of 177Lu‐IBA in normal New Zealand White rabbits. Radiolabeling yields for 177Lu‐IBA proved to be >97% within 30 minutes at 90°C, and its radiochemical purity ensured stability in vitro and in vivo. Furthermore, we found that 177Lu‐IBA is readily soluble in water, showing higher skeletal uptake than 177Lu‐MDP but lower uptake by liver and spleen. The image quality of 177Lu‐IBA was so clear that even after 6 days, analysis was still feasible.
A simplified radiosynthesis of [18F]MK‐6240 for tau PET imaging
Authors: Hopewell Robert , Ross Karen , Kostikov Alexey , Pascoal Tharick A. , Alberti Thais , Lacatus‐Samoila Monica , Soucy Jean‐Paul , Bennacef Idriss , Kobayashi Eliane , Kang Min Su , Rosa‐Neto Pedro , Massarweh Gassan 
Published: November 2018

[18F]MK‐6240 (6‐(fluoro)‐3‐(1H‐pyrrolo[2,3‐c]pyridin‐1‐yl)isoquinolin‐5‐amine) is a highly selective PET radiotracer for the in vivo imaging of neurofibrillary tangles (NFTs). [18F]MK‐6240 was synthesized in one step from its bis‐Boc protected precursor N‐[(tert‐butoxy)carbonyl]‐N‐(6‐nitro‐3‐[1H‐pyrrolo[2,3‐c]pyridin‐1‐yl]isoquinolin‐5‐yl) carbamate in DMSO using [18F] fluoride with TEA HCO3 with step‐wise heating up to 150°C, resulting in an isolated radiochemical yield of 9.8% ± 1.8% (n = 3) calculated from the end of bombardment (5.2% ± 1.0% calculated from the end of synthesis). This new synthetic approach eliminates the acidic deprotection of the bis‐Boc 18F‐labeled intermediate, which reduces the number of operations necessary for the synthesis as well as losses, which occur during deprotection and neutralization of the crude product mixture prior to the HPLC purification. The synthesis was performed automatically with a single‐use cassette on an IBA Synthera+ synthesis module. This synthesis method affords the radioligand with a reliable radiochemical yield, high radiochemical purity, and a high molar activity. [18F]MK‐6240 synthesized with this method has been regularly (n > 60) used in our ongoing human and animal PET imaging studies.
Energetic switch of the proline effect in collision‐induced dissociation of singly and doubly protonated peptide Ala‐Ala‐Arg‐Pro‐Ala‐Ala
Authors: Huo Dayujia , Qin Tai , Zu Lily 
Published: November 2018

AbstractSuppression of the selective cleavage at N‐terminal of proline is observed in the peptide cleavage by proteolytic enzyme trypsin and in the fragment ion mass spectra of peptides containing Arg‐Pro sequence. An insight into the fragmentation mechanism of the influence of arginine residue on the proline effect can help in prediction of mass spectra and in protein structure analysis. In this work, collision‐induced dissociation spectra of singly and doubly charged peptide AARPAA were studied by ESI MS/MS and theoretical calculation methods. The proline effect was evaluated by comparing the experimental ratio of fragments originated from cleavage of different amide bonds. The results revealed that the backbone amide bond cleavage was selected by the energy barrier height of the fragmentation pathway although the strong proton affinity of the Arg side chain affected the stereostructure of the peptide and the dissociation mechanism. The thermodynamic stability of the fragment ions played a secondary role in the abundance ratio of fragments generated via different pathways. Fragmentation studies of protonated peptide AACitPAA supported the energy‐dependent hypothesis. The results provide an explanation to the long‐term arguments between the steric conflict and the proton mobility mechanisms of proline effect.