bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of ampullary carcinoma mixed subtype using a panel of six antibodies and its clinical significance
Authors: Liu Fangfang , Shen Danhua , Ma Yingteng , Song Qiujing , Wang Hanlin 
Published: December 2018

ObjectivesTo investigate the function of immunomarkers CK7, CK20, CK17, CDX2, MUC1, and MUC2 in the identification of primary ampullary carcinoma mixed subtype.MethodsForty‐two cases of primary ampullary carcinoma were performed by immunohistochemical studies. The correlation between the mixed subtype and the other two subtypes and patient survival data was analyzed using the SPSS 16.0 statistical software.ResultsAmong 42 cases, 12 (28.6%) cases were classified as mixed subtype, which showed variable expression patterns: 91.7% (11/12) for CK7, 83.3% (10/12) for CK20; 66.7% (8/12) for CK17, CDX2, and MUC1; and 50% (6/12) for MUC2. Ten (83.3%) mixed types coexpressed four or more immunomarkers. Eight (19%) intestinal subtypes mainly showed a positive expression of CK20, CDX2, and MUC2. Twenty‐two (52.4%) pancreaticobiliary subtypes showed a positive expression of CK7, MUC1, and CK17. Stages III and IV diseases in mixed subtype (25%) and intestinal subtype (25%) were less than pancreaticobiliary subtype(63.6%) (p = 0.039). Follow‐up data appeared to show a better survival rate for patients with mixed subtype than those with pancreaticobiliary subtypes.ConclusionImmunohistochemical staining provided a more reliable means of diagnosing mixed ampulla carcinoma. Accurate subtyping of ampullary carcinoma is clinically important to select effective chemotherapy regimens and to assess disease prognosis.
Effects of game‐product congruity on in‐app interstitial advertising and the moderation of media‐context factors
Authors: Wang Shaojung Sharon , Chou Hsuan‐Yi 
Published: December 2018

AbstractAdvertising has become one of the major sources of game apps revenues and interstitial ads, in particular, are the most used monetization method among the types of in‐game mobile ad formats. Interstitial ads overlaid on top of a mobile game app have more obvious advertising intent and a higher degree of forced exposure and perceived intrusiveness. This study examined how the degree of congruity (high vs. moderate vs. low) between the promoted products in interstitial ads and the mobile game app environment affects consumers’ responses. The moderating effects of media‐context factors (i.e., excited– or calm–happiness game types and game immersion) were further assessed. The results of two experiments revealed that game‐product congruity lead to better consumers’ responses toward ads and advertised products. The positive effects of game‐product congruity were more salient when consumers played calm–happiness games and were less immersed in the game. Theoretical implications on integrating perspectives across disciplines such as schema theory, happiness, and immersion within the media‐context framework, as well as practical suggestions are discussed.
Identification of a de novo splicing variant in the Coffin–Siris gene, SMARCE1, in a patient with Angelman‐like syndrome
Authors: Aguilera Cinthia , Gabau Elisabeth , Laurie Steve , Baena Neus , Derdak Sophia , Capdevila Núria , Ramirez Ariadna , Delgadillo Veronica , García‐Catalan Maria Jesus , Brun Carme , Guitart Miriam , Ruiz Anna 
Published: December 2018

AbstractBackgroundPatients affected by Angelman syndrome (AS) present severe intellectual disability, lack of speech, ataxia, seizures, abnormal electroencephalography (EEG), and a characteristic behavioral phenotype. Around 10% of patients with a clinical diagnosis of AS (AS‐like) do not have an identifiable molecular defect. Some of these patients harbor alternative genetic defects that present overlapping features with AS.MethodsTrio whole‐exome sequence was performed on patient and parent's DNA extracted from peripheral blood. Exome data were filtered according to a de novo autosomal dominant inheritance. cDNA analysis was carried out to assess the effect of the splice site variant.ResultsWe identified a novel heterozygous SMARCE1 splicing variant that leads to an exon skipping in a patient with an Angelman‐like phenotype. Missense variants in the SMARCE1 gene are known to cause Coffin–Siris syndrome (CSS), which is a rare congenital syndrome. Clinical reevaluation of the patient confirmed the presence of characteristic clinical features of CSS, many of them overlapping with AS.ConclusionsTaking into account the novel finding reported in this study, we consider that CSS should be added to the expanding list of differential diagnoses for AS.
Identification of ANLN as a new likely pathogenic gene of branchio‐otic syndrome in a three‐generation Chinese family
Authors: Deng Lisha , Liu Yuanzhen , Xia Wenjun , Hu Jiongjiong , Ma Zhaoxin 
Published: December 2018

AbstractBackgroundBranchio‐oto‐renal (BOR) syndrome is one of the most common autosomal dominant hearing loss syndromes and features clinical and genetic heterogeneity. When there is no renal deformity, this disease can also be called branchio‐otic (BO) syndrome. Though many genes have been reported, there are still many BO syndrome‐related genes to be identified. To identify a hitherto unknown candidate gene causing BO syndrome in a three‐generation Chinese family, clinical, genetic, and functional analyses were employed.MethodsWhole‐exome sequencing (WES) was conducted in three affected family members and two unaffected family members. PCR‐Sanger sequencing was performed in all of the family members for segregation analysis and verification of the candidate variants. PCR‐Sanger sequencing was also employed in 150 healthy people to examine the variants. In silico analysis was used to predict possible changes in the protein structure that may affect the phenotype.ResultsWe identified a heterozygous missense variant in ANLN: NM_018685.4: c.G1105A; NP_061155.2: p.G369R that segregated in the pedigree with an autosomal dominant pattern. No variant was found in the 150 controls and normal family members at this site. The variant c.G1105A was located in a highly conserved F‐actin binding site. The amino acid residue at position 369 in the ANLN protein was highly conserved across different species.ConclusionIn this study, we identified, for the first time, a heterozygous missense variant in ANLN (NM_018685.4: c.G1105A; NP_061155.2: p.G369R) that is likely to be a candidate causative gene of BO syndrome in a specific Chinese family.
Comparison of a low‐cost magneto‐inductive magnetometer with a proton magnetometer: a case study on the Galali iron ore deposit in western Iran
Authors: Shahsavani Hashem 
Published: December 2018

ABSTRACTThe proton magnetometers, also known as the proton precession magnetometers, are among the most widely used instruments for magnetometry surveys, owing to their high resolution which is about 0.01 nT. As the economically attractive ferromagnetic deposits generate a magnetic field anomaly which exceeds 100 nT, this resolution is not needed for detecting such large signals. The recently introduced magneto‐inductive technology has led to the development of a low‐cost magnetometer that can measure the ground magnetic field up to a resolution of around 10 nT. These magnetometers are inexpensive, come in a very small size, and are lightweight compared to the more common magnetometers such as the proton precession type. In this research, a low‐cost magneto‐inductive sensor and a highly precise proton magnetometer are simultaneously utilized at the same profiles on the Galali iron ore deposit in the northwest of Iran. The discrepancy of two measurements is less than 400 nT, often less than 100 nT, while the changes in the total magnetic field on this anomaly are around 7500 nT, which is 11 times larger than the maximum difference between the two measurements. In addition, the value of the regression coefficient between the two measurements for all profiles is more than 0.97. Also, the slope of the fitted line for the two measurements for all profiles is close to one. These results demonstrate the applicability of the new low‐cost magneto‐inductive sensor in magnetic prospecting.
Health disparities: Using policies to rethink our strategies for eliminating the impact of food deserts by focusing on unhealthy dietary patterns
Authors: Wallace Edward V. 
Published: December 2018

For years, we have been interested in understanding the relationship between dietary patterns and diseases, and most recently, we have put efforts toward analyzing the impact of food deserts as they relate to dietary patterns. Unhealthy eating has become an epidemic in low‐income neighborhoods that are considered to be food deserts due to the fact that people are not meeting their recommended daily intake of nutritionally dense foods. Adults should be consuming at least 20 to 35 g of fiber daily, however, many Americans only consume 12 to 17 g of fiber daily at best. Furthermore, as a society, we Americans consume way too much added sugar, saturated fat, and salt. Although there are a number of reasons that unhealthy dietary patterns exist in our society especially in low‐income communities, it is important that we pay particular attention to how food deserts have developed and how they are major contributors to the overall poor health of low‐income Americans. Therefore, the purpose of this is paper is to encourage its audience to rethink how we can implement policies to address the issue of unhealthy dietary patterns by reducing or eliminating food deserts. Specifically, we explore the effect of implementing evidence‐based policies such as nutrition initiatives, corner store initiatives, menu labeling, food assistance programs, and the punitive taxation of sugary beverages and unhealthy foods similar to the punitive taxes placed on tobacco.
Discovery and characterisation of lycorine‐type alkaloids in Lycoris spp. (Amaryllidaceae) using UHPLC‐QTOF‐MS
Authors: Li Aiqian , Du Zhifeng , Liao Mei , Feng Yulin , Ruan Hanli , Jiang Hongliang 
Published: December 2018

AbstractIntroductionLycorine, one of the most common alkaloids in Lycoris spp., is believed to possess pharmacological activity.ObjectiveTo discover and identify lycorine‐type alkaloids in the crude extracts of bulbs from six Lycoris spp. by ultra‐high‐performance liquid chromatography quadrupole time‐of‐flight mass spectrometry (UHPLC‐QTOF‐MS) detection.MethodologyA qualitative analytical method with a data mining strategy was utilised. Based on the fragmentation patterns of standards investigated in positive tandem mass spectrometry (MS/MS) mode, the fragmentation rules of lycorine‐type alkaloids were summarised. These types of alkaloids were additionally classified as different subtypes based on structural features and MS/MS fragmentation patterns, and the diagnostic ions for characterisation of different subtypes of alkaloids were designated.ResultsThirty‐seven lycorine type alkaloids, including 16 previously undescribed compounds, were efficiently screened out and tentatively identified from the crude extracts of six Lycoris spp. Lycoris sprengri may be a preferable species for studying or extracting lycorine‐type alkaloids because of elevated relative concentrations and highest diversity of alkaloids.ConclusionThe UHPLC‐QTOF‐MS and MS/MS data‐mining strategy proved useful for the detection and tentative identification of lycorine‐type alkaloids in bulbs of Lycoris spp. and could be extended to other Amaryllidaceae genera. The consequent profiling of the lycorine‐type alkaloids will be useful in the quality control of raw materials of Lycoris species and the exploration of superior species.
Effects of personality traits and social status on academic achievement: Gender differences
Authors: Janošević Marko , Petrović Boban 
Published: December 2018

AbstractThis study investigates the contribution of personality traits (HEXACO traits and Schizotypy) and social status dimensions (sociometric and perceived popularity) in understanding boys’ and girls’ respective academic achievement. The sample included 163 elementary school students from Serbia, aged 14–15 (87 girls and 76 boys). Regression analysis reveals that personality traits explain a similar amount of academic achievement variance in two gender groups (22% vs. 20% in girls’ favor), but social status proves to be a better predictor of academic achievement for boys (27% vs. 4% in boys’ favor). High Conscientiousness, perceived popularity as well as low extraversion turned out to be related to girls’ academic achievement. Low Schizotypy and Honesty‐Humility, as well as high openness, sociometric, and perceived popularity turned out to be related to boys’ academic achievement. Conscientiousness appears not to be related to boys’ academic achievement. The results are discussed and recommendations for improving educational practices are offered.
Implications of Goods and Services Tax reform on the Make in India initiative: A system dynamics perspective
Authors: Ojha Ravindra , Vrat Prem 
Published: December 2018

AbstractThe paper highlights the implications of Goods and Services Tax (GST), a major tax reform in India launched in July 2017 on the Make in India initiative launched in the year 2014. The GST is expected to trigger its impact on the following eight elements: warehouse efficiency, interstate check‐post operations, informal to formal business transition, interstate business expansion by micro, small, and medium enterprises, simplicity and transparency in tax collection process, logistics cost reductions, ease of doing business in India, and working capital cost realignment, which in turn may accelerate the manufacturing growth in Indian economy. In order to study the impact of the factors of GST on the Make in India initiative and the manufacturing growth, a system dynamics approach has been adopted in this paper. A number of simulation runs carried out using the system dynamics modelling have provided meaningful insights to the policymakers and strategic planners for evolving strategies for manufacturing growth.
Canine non‐epitheliotropic CD4‐positive cutaneous T‐cell lymphoma: a case report
Authors: Kondo Hitoshi , Kagawa Yumiko , Shirota Kinji , Moore Peter F. , Nagata Masahiko 
Published: December 2018

AbstractA 5‐year‐old, spayed female French Bulldog presented with multiple papules on the skin of the scapular area. Histopathological examination of punch biopsy samples revealed dense infiltration of small lymphoid cells in the superficial dermis and in areas surrounding hair follicles. Immunohistochemical analysis indicated that these cells were positive for CD3, CD4, and TCRαβ, but negative for CD1c, CD8α, CD8β, CD11c, CD20, CD45RA, CD90, MHC‐II, and TCRγδ. In addition, CD45 was highly expressed, and the proliferation fraction was very low. Molecular clonality of T‐cell receptor G chains yielded a clonal result. The skin lesions were surgically excised because they had progressed to the lateral front leg. Postoperative clinical course was favorable, and recurrence was not observed until the dog died in a traffic accident, approximately 1 year later.
A model for cellular mechanotransduction and contractility at finite strain
Authors: Bouklas N. , Sakar M. S. , Curtin W. A. 
Published: December 2018

AbstractThe article from this special issue was previously published in ZAMM ‐ Journal of Applied Mathematics and Mechanics / Zeitschrift für Angewandte Mathematik und Mechanik, Volume 98, Issue 10, 2018. For completeness we are including the title page of the article. The full text of the article can be read in Issue 98:10 on Wiley Online Library: https://onlinelibrary.wiley.com/doi/full/10.1002/zamm.201700368
Variations in Seismogenic Thickness Along the Central Alpine Fault, New Zealand, Revealed by a Decade's Relocated Microseismicity
Authors: Michailos Konstantinos , Smith Euan G.C. , Chamberlain Calum J. , Savage Martha K. , Townend John 
Published: December 2018

AbstractThe Alpine Fault is an oblique strike‐slip fault that is known to fail in large magnitude (M7–8) earthquakes, yet it is currently seismically quiescent. We examine the low‐magnitude earthquake activity occurring along the central portion of the Alpine Fault using seismic data from five temporary seismic networks deployed for various lengths of time between late 2008 and early 2017. Starting from continuous seismic data, we detect earthquake arrivals and construct the longest and most extensive microearthquake catalog for the central Alpine Fault region to date, containing 9,111 earthquakes. This enables us to study the distribution and characteristics of the seismicity in unprecedented detail. Earthquake locations are constrained by high‐quality automatic and manual picks, and we perform relocations using waveform cross‐correlation to better constrain hypocenters. We have derived a new local magnitude scale calibrated by Mw values. Magnitudes range between ML−1.2 and 4.6, and our catalog is complete above ML 1.1. Earthquakes mainly occur southeast of the Alpine Fault (in the hanging wall) and exhibit low magnitudes. We observe a lack of seismicity beneath Aoraki/Mount Cook, which we associate with high uplift rates and high heat flow. Seismogenic cutoff depths vary along the strike of the Alpine Fault from 8 km, beneath the highest topography, to 20 km in the adjacent areas.
Dynamical Response of Changjiang River Plume to a Severe Typhoon With the Surface Wave‐Induced Mixing
Authors: Zhang Zhiwei , Wu Hui , Yin Xunqiang , Qiao Fangli 
Published: December 2018

AbstractTyphoons (or hurricanes) are the most energetic atmospheric forcing acting on coastal waters. Here in this study, we investigated the response of the summertime Changjiang River plume to a typical typhoon, Chan‐hom (1509), with a combination of field observation and numerical simulation. Surface wave‐induced mixing was considered in the model configuration. The results showed that the typical offshore‐extending summer Changjiang River plume completely disappeared under the influence of typhoon wind. Instead, it extended southward along the Zhejiang and Fujian (Zhe‐Min) coast as a typical wintertime Changjiang River plume. The along‐shelf plume extension lasted for extra ~10 days after the typhoon passage, until another strong weather event came. The competition between wind‐driven current and buoyancy‐driven current dominated the recovery of the Changjiang River plume. Through calculation, we found that the freshwater transported to the Zhe‐Min Coastal Water reached ~4.7 × 1010 m3 as influenced by typhoon Chan‐hom, which was ~5% of the total Changjiang River discharge in 2015 or ~12% of the total dry season Changjiang River discharge (October‐April) when the majority of Changjiang River plume extended to Zhe‐Min Coastal Water. The remote sensing data of chlorophyll‐α from Geostationary Ocean Color Imager also showed that significant algal bloom occurred when the southward extending Changjiang River plume retreated. Surface wave‐induced mixing caused by typhoon wind was found to be important in destroying the vertical plume stratification and elongating the recovery processes from the typhoon influence.
Increased α2-6 sialylation of endometrial cells contributes to the development of endometriosis
Authors: Choi Hee-Jin , Chung Tae-Wook , Choi Hee-Jung , Han Jung Ho , Choi Jung-Hye , Kim Cheorl-Ho , Ha Ki-Tae 
Published: December 2018

AbstractEndometriosis is a disease characterized by implants of endometrial tissue outside the uterine cavity and is strongly associated with infertility. Focal adhesion of endometrial tissue to the peritoneum is an indication of incipient endometriosis. In this study, we examined the effect of various cytokines that are known to be involved in the pathology of endometriosis on endometrial cell adhesion. Among the investigated cytokines, transforming growth factor-β1 (TGF-β1) increased adhesion of endometrial cells to the mesothelium through induction of α2-6 sialylation. The expression levels of β-galactoside α2-6 sialyltransferase (ST6Gal) 1 and ST6Gal2 were increased through activation of TGF-βRI/SMAD2/3 signaling in endometrial cells. In addition, we discovered that terminal sialic acid glycan epitopes of endometrial cells engage with sialic acid-binding immunoglobulin-like lectin-9 expressed on mesothelial cell surfaces. Interestingly, in an in vivo mouse endometriosis model, inhibition of endogenous sialic acid binding by a NeuAcα2-6Galβ1-4GlcNAc injection diminished TGF-β1-induced formation of endometriosis lesions. Based on these results, we suggest that increased sialylation of endometrial cells by TGF-β1 promotes the attachment of endometrium to the peritoneum, encouraging endometriosis outbreaks.
Reduced TCA cycle rates at high hydrostatic pressure hinder hydrocarbon degradation and obligate oil degraders in natural, deep-sea microbial communities
Authors: Scoma Alberto , Heyer Robert , Rifai Ridwan , Dandyk Christian , Marshall Ian , Kerckhof Frederiek-Maarten , Marietou Angeliki , Boshker Henricus T S , Meysman Filip J R , Malmos Kirsten G , Vosegaard Thomas , Vermeir Pieter , Banat Ibrahim M , Benndorf Dirk , Boon Nico 
Published: December 2018

Abstract
Petroleum hydrocarbons reach the deep-sea following natural and anthropogenic factors. The process by which they enter deep-sea microbial food webs and impact the biogeochemical cycling of carbon and other elements is unclear. Hydrostatic pressure (HP) is a distinctive parameter of the deep sea, although rarely investigated. Whether HP alone affects the assembly and activity of oil-degrading communities remains to be resolved. Here we have demonstrated that hydrocarbon degradation in deep-sea microbial communities is lower at native HP (10 MPa, about 1000 m below sea surface level) than at ambient pressure. In long-term enrichments, increased HP selectively inhibited obligate hydrocarbon-degraders and downregulated the expression of beta-oxidation-related proteins (i.e., the main hydrocarbon-degradation pathway) resulting in low cell growth and CO2 production. Short-term experiments with HP-adapted synthetic communities confirmed this data, revealing a HP-dependent accumulation of citrate and dihydroxyacetone. Citrate accumulation suggests rates of aerobic oxidation of fatty acids in the TCA cycle were reduced. Dihydroxyacetone is connected to citrate through glycerol metabolism and glycolysis, both upregulated with increased HP. High degradation rates by obligate hydrocarbon-degraders may thus be unfavourable at increased HP, explaining their selective suppression. Through lab-scale cultivation, the present study is the first to highlight a link between impaired cell metabolism and microbial community assembly in hydrocarbon degradation at high HP. Overall, this data indicate that hydrocarbons fate differs substantially in surface waters as compared to deep-sea environments, with in situ low temperature and limited nutrients availability expected to further prolong hydrocarbons persistence at deep sea.