bluuebunny/crossref_metadata_2025_split huggingface dataset:
Constructing 0D FeP Nanodots/2D g‐C3N4 Nanosheets Heterojunction for Highly Improved Photocatalytic Hydrogen Evolution
Authors: Zhao Chengxiao , Tang Hua , Liu Wei , Han Chenhui , Yang Xiaofei , Liu Qinqin , Xu Jingsan 
Published: September 2019

AbstractTwo‐dimensional polymeric graphitic carbon nitride (2D g‐C3N4) nanostructures have emerged as a very promising family of photocatalysts for hydrogen evolution from water. Nonetheless, fast recombination of photogenerated charge carriers and the sluggish kinetics of hydrogen evolution reaction (HER) result in unsatisfied solar‐to‐hydrogen efficiency. It is thus greatly encouraging to explore low‐cost and high‐performance co‐catalysts to accelerate the charge transport and to improve the catalytic performance. In this work, for the first time, we report on the fabrication of ultra‐small zero‐dimensional (0D) FeP nanodots anchored on layered 2D g‐C3N4 nanosheets with well‐defined nanostructures and intimate 0D/2D contact interface. The outstanding feature of the material is that the obtained multidimensional heterojunction photocatalysts reveal highly efficient visible‐light‐responsive HER performance in the absence of noble metals. Under an optimal percentage of 4 wt. % FeP, the FeP/g‐C3N4 hybrid showed a remarkable hydrogen‐evolving rate of 215 μmol g−1 h−1 and excellent recycling stability, surpassing noble‐metal Pt modified g‐C3N4 (155 μmol g−1 h−1). The enhancement in HER activity is attributed to the synergistic effects of FeP nanocrystals on the improvement of light‐harvesting property in the visible light region and the acceleration of charge carrier dynamics by well‐constructed interfaces.
Numerical Investigation of Degassing Behavior of Highly Viscous Non‐Newtonian Fluids under Stirring
Authors: Ma Qianli , Shang Chunli , Zhang Guojun , Fang Haisheng 
Published: September 2019

AbstractA method for accelerating bubble removal in non‐Newtonian liquids by forced stirring is proposed. Numerical simulations based on an Eulerian‐Eulerian multiphase model are performed to test its feasibility for various liquids including shear‐thickening, shear‐thinning, and Newtonian ones. Effects of the rotating speed of the stirrer and rheological properties of liquids on the degassing efficiency are evaluated, and the influencing mechanisms are analyzed in detail. The feasible regions of degassing in shear‐thinning liquids covering a certain range of viscosities are determined, from which one can easily decide the optimal rotating speed given the viscosity of liquids. The results provide a new idea of efficient degassing in shear‐thinning liquids for researchers and engineers.
Aromatic and Antiaromatic Pathways in Triphyrin(2.1.1) Annelated with Benzo[b]heterocycles
Authors: Bartkowski Krzysztof , Dimitrova Maria , Chmielewski Piotr J. , Sundholm Dage , Pawlicki Miłosz 
Published: September 2019

AbstractUnderstanding of the aromatic properties and magnetically induced current densities of highly conjugated chromophores is important when designing molecules with strongly delocalized electronic structure. Linear extension of the triphyrin(2.1.1) skeleton with an annelated benzo[b]heterocycle fragment modifies the aromatic character by extending the electron delocalization pathway. Two‐electron reduction leads to an antiaromatic triphyrin(2.1.1) ring and an aromatic benzo[b]heterocycle subunit. Current‐density calculations provide detailed information about the observed pathways and their strengths.
Performance of the Afirma genomic sequencing classifier versus gene expression classifier: An institutional experience
Authors: Wei Shuanzeng , Veloski Colleen , Sharda Pankaj , Ehya Hormoz 
Published: September 2019

BackgroundThe use of fine‐needle aspiration (FNA) to triage thyroid nodules has resulted in a significant reduction in thyroid surgery. However, approximately one‐third of FNA specimens fall into the “indeterminate” category. The Afirma gene expression classifier (GEC) has been used to identify benign nodules with a high sensitivity and negative predictive value. However, the specificity and positive predictive value of the “suspicious” category are low. The updated Afirma genomic sequencing classifier (GSC) has been reported to demonstrate increased specificity while maintaining a high sensitivity and negative predictive value.MethodsThe authors retrospectively investigated 272 indeterminate thyroid FNA specimens (Bethesda categories III and IV) from nodules measuring >1 cm using the Afirma GEC or GSC tests (July 2012‐January 2019).ResultsOf the 194 nodules tested using the Afirma GEC, a benign result was obtained in 88 cases (45.4%). In comparison, 52 of 78 FNA samples (66.7%) tested using GSC yielded a benign result (P = .002). In the GEC group, there were 31 cases with oncocytic cytology, 5 of which (16.1%) were benign on Afirma and 26 of which (83.9%) were suspicious on Afirma. In contrast, in the GSC group, there were 10 cases with oncocytic cytology, 8 of which (80%) were benign on Afirma and only 2 of which (20%) were found to be suspicious on Afirma (P < .001). The positive predictive value of the GSC group (57.1%) was higher than that of the GEC group (36.7%); however, there was no statistical significance noted (P = .15).ConclusionsA larger percentage of indeterminate thyroid FNA specimens were classified as benign using the Afirma GSC compared with the Afirma GEC, especially among samples with oncocytic features. The Afirma GSC appears to have a higher benign call rate compared with the Afirma GEC.
Rapid Tagging of Human Proteins with Fluorescent Reporters by Genome Engineering using Double‐Stranded DNA Donors
Authors: Paix Alexandre , Rasoloson Dominique , Folkmann Andrew , Seydoux Geraldine 
Published: September 2019

AbstractTagging proteins with fluorescent reporters such as green fluorescent protein (GFP) is a powerful method to determine protein localization, especially when proteins are tagged in the endogenous context to preserve native genomic regulation. However, insertion of fluorescent reporters into the genomes of mammalian cells has required the construction of plasmids containing selection markers and/or extended sequences homologous to the site of insertion (homology arms). Here we describe a streamlined protocol that eliminates all cloning steps by taking advantage of the high propensity of linear DNAs to engage in homology‐directed repair of DNA breaks induced by the Cas9 RNA‐guided endonuclease. The protocol uses PCR amplicons, or synthetic gene fragments, with short homology arms (30‐40 bp) to insert fluorescent reporters at specific genomic locations. The linear DNAs are introduced into cells with preassembled Cas9‐crRNA‐tracrRNA complexes using one of two transfection procedures, nucleofection or lipofection. The protocol can be completed under a week, with efficiencies ranging from 0.5% to 20% of transfected cells depending on the locus targeted. © 2019 The Authors.
Scale and sense of place among urban dwellers
Authors: Ardoin Nicole M. , Gould Rachelle K. , Lukacs Heather , Sponarski Carly C. , Schuh Janel S. 
Published: September 2019

AbstractPlace connections are core to being human: Every person lives in, and thus has direct experience of, at least one place and likely of numerous places throughout a lifetime. Sense of place—or the meanings, knowledge, and bonds that arise from the biophysical, social, and political–economic aspects of places—in turn influences people's interactions with those places. Of particular interest to researchers, practitioners, and policymakers, such interactions can impact place‐protective, stewardship, or conservation behaviors. However, how sense of place develops and what it represents is shifting in today's rapidly urbanizing, globalizing world. Especially when considering the integrated social–ecological context, questions related to how sense of place forms and is enacted in urban settings and at a range of geographic scales are challenging to study. Our study addresses this dynamic space: We examined how people's place connections intersect with their notions of geographic scale and levels of urbanity. Specifically, we conducted a 1201‐person randomized telephone survey in the San Francisco Bay Area ecoregion of California, USA, to explore how sense of place varies by (1) the scale of what people consider to be their place, and (2) the urbanity of where people live. In comparison with respondents who perceived their place as the larger‐scale ecoregion, we found that respondents who perceived their place as primarily focused on the urban area rated their connection to the biophysical aspects of place (the plants, animals, and landscape‐related elements) lower. Similarly, overall, respondents who lived in urban areas rated their connections to the biophysical aspects of place lower than did respondents who lived in non‐urban areas. Our findings suggest the importance of encouraging conceptualizations of place at broader geographic scales and, particularly, of supporting notions of urban spaces that stretch beyond urban boundaries. We also call for supporting increased engagement with urban nature, especially among residents of urban areas.
Efficacy of immunosuppressants with bridge vasodilator therapy in severe lupus erythematosus‐associated pulmonary arterial hypertension
Authors: Sanges Sébastien , Savale Laurent , Lamblin Nicolas , Rémy‐Jardin Martine , Humbert Marc , Sobanski Vincent 
Published: September 2019

AbstractOptimal management of systemic lupus erythematosus (SLE)‐associated pulmonary arterial hypertension (PAH) remains unclear. Our observation describes the case of a 31‐year‐old SLE patient presenting with cardiogenic shock revealing severe PAH, in which a therapeutic scheme combining immunosuppressants (pulse cyclophosphamide and corticosteroids) and PAH‐specific drugs (bosentan, tadalafil, and epoprostenol) led to a complete normalization of pulmonary haemodynamics and allowed a progressive weaning of PAH vasodilators. This case report supports the efficacy of immunosuppressants and use of PAH‐specific therapy as a bridge therapy in severe SLE‐PAH. Further studies on larger population are required to confirm these findings.
Effect of the elapsed time between sampling and formalin fixation on the N‐glycosylation profile of mouse tissue specimens
Authors: Donczo Boglarka , Kiraly Gabor , Guttman Andras 
Published: September 2019

AbstractFormalin‐fixed, paraffin‐embedded (FFPE) samples are generally used for histology‐study, however, they also possess important molecular diagnostics information. While it has been reported that the N‐glycan moieties of glycoproteins is not affected by the FFPE process, no information is available about the effect of the elapsed time between sampling and fixation on the resulting N‐glycosylation profile. In this study, lung, brain, heart, spleen, liver, kidney, and intestine mouse tissue specimens were used for N‐glycan profiling analysis and the elapsed sampling time effect was investigated with the lung tissue. N‐glycan extraction from the tissue samples was performed by glycoprotein retrieval from the FFPE specimens using radioimmunoprecipitation assay (RIPA) buffer followed PNGase F digestion. The released oligosaccharides were fluorophore labeled and analyzed by capillary electrophoresis‐laser induced fluorescent detection (CE‐LIF). N‐glycosylation profiles of freshly collected lung‐tissue samples (zero time point), as well as 1 and 2 h after sampling were compared by carbohydrate profiling and exoglycosidase treatment based deep glycomic analysis. It was found that up to two hours of room temperature storage of tissue specimens apparently did not cause changes in the N‐glycosylation profiles of complex carbohydrates, but resulted in considerable decrease in the amount of linear glucose oligomers and high mannose type glycans present in the samples.
Cognitive spectrum access with energy harvesting primary user and best secondary user selection
Authors: Zhai Chao , Zheng Lina , Yu Zhiyuan 
Published: September 2019

AbstractEnergy harvesting and spectrum sharing are promising techniques to improve the energy and spectral efficiency of wireless networks. In this work, we propose a two‐phase spectrum sharing scheme with multiple secondary users (SUs) coexisting with an energy harvesting primary user (PU). In Phase I, along with a power beacon transferring wireless power to PU, an SU is selected to transmit data using its maximal power, from which the interference can help charge PU. In Phase II, PU will transmit data using its harvested energy and, meanwhile, another SU is selected to transmit data with a controlled power under an interference constraint of primary system. Particularly, if PU has not harvested any energy in Phase I, it will keep silent in Phase II, and an SU is selected to transmit using its maximal power. Numerical results show that our scheme can achieve a reasonably good throughput for SUs while improving the performance of primary system.
Synthesis and luminescent properties of polymer‐silica multilayer‐encapsulated perovskite quantum dots for optoelectronics
Authors: Liu Shuang‐De , Chen Teng‐Ming 
Published: September 2019

AbstractHydrophobic all‐inorganic perovskite quantum dots (IPQDs) CsPbX3 (X = Cl, Br, and I) were synthesized using conventional solution methods. IPQDs are potential materials for lasers, solar cells, and displays because of their narrow full width at half‐maximum, optically tunable wavelength, and great photoluminescence quantum yield. Their shortcomings are structurally unstable and moisture sensitive. To overcome the poor stability, we have developed a chemical modification to form GQD + PMAO@SiO2 that enhances their photoluminescence quantum yield (from 60 to ~90%), thermal stability (from 60 to ~90°C), and photochemical stability. The combination of the green‐emitting GQD + PMAO@SiO2 and red‐emitting RQD + PMAO with a 450 nm blue InGaN‐based LED chip was used to fabricate a white light emitting diode with the correlated color temperature (CCT) value of 6,943 K and Commission Internationale del'Eclairage of (0.312, 0.290).
Melatonin protects against defects induced by malathion during porcine oocyte maturation
Authors: Chen Li , Zhang Jing‐Jing , Zhang Xia , Liu Xiaoli , Zhao Shuhong , Huo Li‐Jun , Zhou Jilong , Miao Yi‐Liang 
Published: September 2019

AbstractMalathion (MAL) is a common organophosphorus pesticide and affects both animal and human reproduction. However, the mechanisms regarding how MAL affects the mammalian oocyte quality and how to prevent it have not been fully investigated. In this study, we used porcine oocyte as a model and proved that MAL impaired porcine oocyte quality in a dose‐dependent manner during maturation. MAL decreased the first polar body extrusion, disrupted spindle assembly and chromosome alignment, impaired cortical granules (CGs) distribution, and increased reactive oxygen species (ROS) level in oocytes. RNA‐seq analysis showed that MAL exposure altered the expression of 2,917 genes in the porcine maturated oocytes and most genes were related to ROS, the lipid droplet process, and the energy supplement. Nevertheless, these defects could be remarkably ameliorated by adding melatonin (MLT) into the oocyte maturation medium. MLT increased oocyte maturation rate and decreased the abnormities of spindle assembly, CGs distribution and ROS accumulation in MAL‐exposed porcine oocytes. More important, MLT upregulated the expression of genes related to lipid droplet metabolism (PPARγ and PLIN2), decreased lipid droplet size and lipid peroxidation in MAL‐exposed porcine oocytes. Finally, we found that MLT increased the blastocysts formation and the cell numbers of blastocysts in MAL‐exposed porcine oocytes after parthenogenetic activation, which was mediated by reduction of ROS levels and maintaining lipid droplet metabolism. Taken together, our results revealed that MLT had a protective action against MAL‐induced deterioration of porcine oocyte quality.
New‐onset atrial fibrillation after atrial ischemia
Authors: Kondo Yusuke , Kobayashi Yoshio 
Published: September 2019

See articles in Journal Of ArhythmiaDOI: doi.org/10.1002/joa3.12470Author's response to Letter to the Editor: Reply to the letter by Kondo et al: Mechanisms for new‐onset atrial fibrillation in patients with acute coronary syndrome
Antimicrobial Blue Light Inactivation of Microbial Isolates in Biofilms
Authors: Ferrer‐Espada Raquel , Wang Ying , Goh Xueping Sharon , Dai Tianhong 
Published: September 2019

Background and ObjectivesBiofilms cause more than 80% of infections in humans, including more than 90% of all chronic wound infections and are extremely resistant to antimicrobials and the immune system. The situation is exacerbated by the fast spreading of antimicrobial resistance, which has become one of the biggest threats to current public health. There is consequently a critical need for the development of alternative therapeutics. Antimicrobial blue light (aBL) is a light‐based approach that exhibits intrinsic antimicrobial effect without the involvement of exogenous photosensitizers. In this study, we investigated the antimicrobial effect of this non‐antibiotic approach against biofilms formed by microbial isolates of multidrug‐resistant bacteria.Study Design/Materials and MethodsMicrobial isolates of Acinetobacter baumannii, Candida albicans, Escherichia coli, Enterococcus faecalis, MRSA, Neisseria gonorrhoeae, Pseudomonas aeruginosa, and Proteus mirabilis were studied. Biofilms were grown in microtiter plates for 24 or 48 hours or in the CDC biofilm reactor for 48 hours and exposed to aBL at 405 nm (60 mW/cm2, 60 or 30 minutes). The anti‐biofilm activity of aBL was measured by viable counts.ResultsThe biofilms of A. baumannii, N. gonorrhoeae, and P. aeruginosa were the most susceptible to aBL with between 4 and 8 log10 inactivation after 108 J/cm2 (60 mW/cm2, 30 minutes) or 216 J/cm2 (60 mW/cm2, 60 minutes) aBL were delivered in the microplates. On the contrary, the biofilms of C. albicans, E. coli, E. faecalis, and P. mirabilis were the least susceptible to aBL inactivation (−0.30, −0.24, −0.84, and −0.68 log10 inactivation, respectively). The same aBL treatment in biofilms developed in the CDC biofilm reactor, caused −1.68 log10 inactivation in A. baumannii and −1.74 and −1.65 log10 inactivation in two different strains of P. aeruginosa.ConclusionsaBL exhibits potential against pathogenic microorganisms and could help with the significant need for new antimicrobials in clinical practice to manage multidrug‐resistant infections. Lasers Surg. Med. © 2019 Wiley Periodicals, Inc.
Role of glucocorticoid signaling in urothelial tumorigenesis: Inhibition by prednisone presumably through inducing glucocorticoid receptor transrepression
Authors: Ide Hiroki , Inoue Satoshi , Mizushima Taichi , Kashiwagi Eiji , Zheng Yichun , Miyamoto Hiroshi 
Published: September 2019

AbstractGlucocorticoids, including dexamethasone (DEX) and prednisone (PRED), have been prescribed in patients with neoplastic disease as cytotoxic agents or comedications. Nonetheless, it remains uncertain whether they have an impact on the development of bladder cancer. We, therefore, assessed the functional role of the glucocorticoid‐mediated glucocorticoid receptor (GR) signaling in urothelial tumorigenesis. Tumor formation was significantly delayed in xenograft‐bearing mice with implantation of control bladder cancer UMUC3 cells or nonneoplastic urothelial SVHUC cells undergoing malignant transformation induced by a chemical carcinogen 3‐methylcholanthrene (MCA), compared with respective GR knockdown xenografts. Using the in vitro system with MCA‐SVHUC cells, we screened 11 GR ligands, including DEX, and found significant inhibitory effects of PRED on their neoplastic transformation. The effects of PRED were restored by a GR antagonist RU486 in GR‐positive MCA‐SVHUC cells, while PRED failed to inhibit the neoplastic transformation of GR knockdown cells. Significant decreases in the expression levels of oncogenes (c‐Fos/c‐Jun) and significant increases in those of a tumor suppressor UGT1A were seen in MCA‐SVHUC‐control cells (vs GR‐short hairpin RNA) or PRED‐treated MCA‐SVHUC‐control cells (vs mock). In addition, N‐butyl‐N‐(4‐hydroxybutyl) nitrosamine induced bladder cancer in all of eight mock‐treated mice vs seven (87.5%) of DEX‐treated (P = .302) or four (50%) of PRED‐treated (P = .021) animals. Finally, DEX was found to considerably induce both transactivation (activation of glucocorticoid‐response element mediated transcription and expression of its targets) and transrepression (suppression of nuclear factor‐kappa B transactivation and expression of its regulated genes) of GR in SVHUC cells, while PRED more selectively induced GR transrepression. These findings suggest that PRED could prevent urothelial tumorigenesis presumably via inducing GR transrepression.
Wheat Protein Hydrolysate Fortified With l‐Arginine Enhances Satiation Induced by the Capsaicinoid Nonivamide in Moderately Overweight Male Subjects
Authors: Stoeger Verena , Lieder Barbara , Riedel Johanna , Schweiger Kerstin , Hoi Julia , Ruzsanyi Veronika , Klieber Martin , Rust Petra , Hans Joachim , Ley Jakob P , Krammer Gerhard E , Somoza Veronika 
Published: September 2019

ScopeIncreasing the intake of satiety‐enhancing food compounds represents a promising strategy for maintaining a healthy body weight. Recently, satiating effects for the capsaicinoid nonivamide have been demonstrated. As various proteins and amino acids have also been demonstrated to decrease energy intake, oral glucose tolerance test (oGTT)‐based bolus interventions of 75 g glucose + 0.15 mg nonivamide (NV control) are tested with/without combination of a wheat protein hydrolysate (WPH: 2 g) and/or l‐arginine (ARG: 3.2 g) for their satiating effects in 27 moderately overweight male subjects.Methods and ResultsCompared to NV control intervention, ARG and WPH + ARG treatment both reduce (p < 0.01) total calorie intake from a standardized breakfast by –5.9 ± 4.15% and –6.07 ± 4.38%, respectively. For the WPH + ARG intervention, increased mean plasma serotonin concentrations (AUC: 350 ± 218), quantitated by ELISA, and delayed gastric emptying, assessed by 13C‐Na‐acetate breath test (−2.10 ± 0.51%, p < 0.05), are demonstrated compared to NV control. Correlation analysis between plasma serotonin and gastric emptying reveals a significant association after WPH ± ARG intervention (r = –0.396, p = 0.045).ConclusionCombination of WPH and ARG enhances the satiating effect of nonivamide, providing opportunities to optimize satiating food formulations by low amounts of the individual food constituents.
Simulation of the stick‐slip motion of an oscillatory roller
Authors: Paulmichl Ivan , Adam Christoph , Adam Dietmar 
Published: September 2019

AbstractA three degrees‐of‐freedom (3DOF) lumped parameter model (LPM) of the oscillatory roller‐soil interaction system is presented that captures the stick‐slip motion of the drum during near‐surface compaction of non‐cohesive soils. The deformed soil below the drum is predefined as a rigid curved asymmetric guiding track (i.e. the compaction process in the soil is not simulated), supported by a vertical and horizontal Kelvin‐Voigt element, to predict also the vertical drum accelerations as observed in situ. The solution of this model, found by switching between the sets of highly nonlinear ODEs for the stick and slip phase, is compared with recorded and numerically simulated (Finite Element model) drum responses.