bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of carbapenemases enterobacteriaceae producing gene blaVIM in clinical isolates
Authors: Amalia Ella , Sabrina Tia , Yuwono  , Patricia Venny , Husna Radhiyatul , Augusta Rosdah Ayeshah , Safyudin  
Published: July 2019

Abstract
Carbapenemase enzymes play the most important role in the existence of CRE (Carbapenem-resistant Enterobacteriaceae). VIM is one of the carbapenemase enzymes encoded by the blaVIM gene. The genes which are partially located in genetic mobile elements may facilitate the spread of the resistance to other bacteria. The aim of this study was to identify the blaVIM gene in CRE isolated from infected patients in dr. Mohammad Hoesin Palembang. This descriptive observational laboratory study using 709 samples of Enterobacteriaceae isolated from various specimens of infected patients at dr. Mohammad Hoesin Palembang during the September-November period which would be identified as CRE using Vitek 2 Compact. 21 isolates which have been identified by CRE was taken by purposive sampling method to detect blaVIM gene with conventional polymerase chain reaction (PCR). The PCR product was electrophoresed and visualized to see a single 390bp DNA band. Out of 709 isolates of Enterobacteriaceae, 88 (12.4%) isolates were identified as CRE. Only 21 isolates of CRE performed PCR. Three (14.3%) isolates carrying the blaVIM gene which found in Enterobacter sp. (N=2; 66.7%) and Klebsiella pneumoniae (n=1; 33.3%). In this study 14.3%, Enterobacteriaceae with CRE phenotype has blaVIM gene genotype.
Relationship of estrogen beta (ERβ) receptor genes polymorphism with epithelial ovarian cancer
Authors: Andreinieelel Ria , Nita Sri , Saleh Irsan 
Published: July 2019

Abstract
Ovarian cancer is the leading cause of death among all uterine diseases. Ovarian cancer is affected by exogenous and endogenous estrogen factors. Beta estrogen receptors are predominant estrogen receptors in the normal ovary. Polymorphisms in the beta estrogen receptor gene (ERβ) can affect the risk of epithelial ovarian cancer through regulation of cell proliferation and apoptosis. The purpose of this study was to determine the relationship of beta estrogen receptor gene polymorphism (ERβ) with epithelial ovarian cancer incidence. Method: a case-control study, there were 60 samples. A single nucleotide polymorphism in the beta estrogen receptor (RsaI/rs1256049) is selected. Genotyping using PCR-RFLP. Data were analyzed by Chi-square, considered significant if the p-value is less than 0.05. Results: there is no significant difference in the characteristics of respondents. Genotypic frequency in the case group was 56.7% GG, 43.3% GA/AA and control group was 23.2% GG, 76.7% GA/AA. The frequency of alleles in the case group was 78.3% G, 21.7% A and control which was 50% G, 50% A allele. ERβ polymorphism is associated with epithelial ovarian cancer (p 0.018). Allele A in RsaI was associated with epithelial ovarian cancer (p 0.002). Polymorphism of the beta estrogen receptor gene associated with epithelial ovarian cancer.
Potential dual effect anti-inflammatory and anti-platelet of cogon grass ethanol extract on diabetic mice a preliminary study
Authors: Anggraeni Neni , Syamsunarno Mas Rizky A A , Widyastuti Rini , Puspitasari Irma M , Praptama Suhendra 
Published: July 2019

Abstract
Cogon grass is traditional medicine empirically used in nephritis, fever, hypertension, dyspnea, epitaxy, as a hepatoprotector agent, and has function to lower cholesterol and blood glucose. The compound of cogon grass is potentially served as an herbs medicine. But, the effect on haematology profile is still well unknown. We demonstrated the effect of cogon grass ethanol extract in mice model diabetic induced with STZ. Eight weeks old of male balb/c mice were injected intraperitoneally with STZ dose 130 mg/Kg BW. Seven days later, after DM confirmed, mice were given ethanol extract of cogon grass with dose 90 mg/KGBW (treatment group 1) and dose 115 mg/Kg BW (treatment group 2). After 14 days of extract gavage, haematology profile were estimated using the direct current detection method. We found there is no anemia occur in diabetic mice. But, the platelet and WBCs, were tend to increase in diabetic control group and treatment group 1, in contrast in treatment group 2 was tend to decrease. In conclusion, we suggested that the extract ethanol of cogon grass suppress the leukocyte and platelet count. It has potentially effect as anti-inflammatory and antiplatelet on diabetic mice.
Association of vascular endothelial growth factor gene +405 C>G and -460 C>T polymorphism with diabetic foot ulcer in Indonesia
Authors: Muhammad Dahlan Kemas , Pratama Dedy , Muradi Akhmadu , Anita Suryandari Dwi , Yunaini Luluk , Boediningsih Setyawati 
Published: July 2019

Abstract
Background, the greatest risk factor for Diabetic foot ulcer (DFU) is neuropathy. Vascular endothelial growth factor (VEGF) gene is a gene encodes a protein vascular endothelial growth factor (VEGF), which has a function of angiogenesis and neurogenesis. VEGF plays a role in neuropathy, angiopathy and wound healing in DFU. Methods: Case-control study, case is types 2 DM with DFU and control is type 2 DM without DFU, Polymerase Chain Reaction-Restriction Fragment length polymorphism was done to find genotype polymorphism of VEGF gene. Results: Genotype GG VEGF + 405C> G does not have a significant association with DFU in DM patients (GG + CG / CC; OR; 0.52, 95% CI; 0.15 to 1.73 p; 0.289). G allele is proposed as a protective factor in DFU (OR; 0.86, 95% CI 0.57 to 1.28, and p; 0.456). Genotype TT from VEGF gene -460 C> T; have no significant association with DFU (TT + CT / CC; OR; 0.97, 95% CI; 0.41 to 2.26 and p; 0.942). T allele is predicted as protective factor in DFU (OR; 0.90, 95% CI; 0.59 to 1.37 and p; 0,641). Conclusion: G alleles and T alleles are predicted as a protective factor in DM patients associated with DFU.
Effects of sodium hyaluronate compared with steroid eye drop against the degree of canalicular stenosis in breast cancer patients treated with docetaxel
Authors: Erna Riani , Qodir Nur , Ristania  
Published: July 2019

Abstract
Canalicular stenosis caused by inflammatory processes in the mucosa of canalicular causing thickening of the squamous epithelium, subsequently parakeratosis and hyperkeratosis of the squamous epithelium. The aim of this study was to assess the effects of sodium hyaluronate compared with steroid eye drop against the degree of canalicular stenosis in breast cancer patients treated with docetaxel chemotherapeutic agents. This study was a clinical trial with the randomized controlled trial (RCT) of 80 sample during the period August-October 2017. Bivariate analyzes used chi-square and McNemar test, and multivariate analysis used logistic regression with the backward method. A total of 20 samples with moderate degree stenosis became mild degree and 36 samples with mild degree stenosis became not stenosis after treated with sodium hyaluronate eye drops. A total of 16 samples with moderate degree stenosis became mild degree and 36 samples with mild degree stenosis became not stenosis after treated with steroid eye drops. McNemar test showed a significant difference in the degree of stenosis after treated with sodium hyaluronate (p=0.039) and steroid eye drops (p=0.000), but chi-square test showed no significant difference in the degree of stenosis between sodium hyaluronate and steroid (p=0.302). Multivariate analysis showed that age, duration of administration and dose of docetaxel effects in canalicular stenosis. Administration of sodium hyaluronate and steroid eye drops can improve the degree of canalicular stenosis.
Correlation between the percentage of memory T cells and IFN-γ level in patients with systemic lupus erythematous
Authors: Handono Kusworini , Ulfah Fahrina , Octaviani Hanani , Kalim Handono 
Published: July 2019

Abstract
Systemic Lupus Erythematosus (SLE) is a chronic systemic autoimmune disease with diverse clinical and autoantibodies expression. Immune senescence is mostly affect the adaptive immune system, characterized by decrease of naïve T cells and increase of memory T cells. The aim of this study was to analyze the association between percentage of memory helper T lymphocytes (CD4CD45RO+Th), memory cytotoxic T lymphocyte (CD8CD45RO+Tc) and IFN-γ serum levels in patients with SLE. Subjects were 61 female SLE patients, 16 -56 years old, from Rheumatology Clinic Dr. Saiful Anwar Hospital Malang (SLICC classification criteria, 2012). Severity of disease activity were assessed using MEXSLEDAI score, percentage of memory Th cells, memory Tc cells were examined using flow cytometry and IFN-γ serum level were measured by ELISA. The percentages of memory (CD4+CD45RO+) Th cells, memory (CD8+CD45RO+) Tc cells and IFN-γ levels were significantly higher in SLE patients with SLEDAI score > 5 compared with those having SLEDAI score <5 (p = 0.000; p = 0.000; p = 0.032). There were a positive correlation between percentages of memory (CD4+CD45RO+) Th cells (p = 0.003, r = 0.453) and memory (CD8+CD45RO+) Tc cell (p = 0.045, r = 0.284) with IFN-γ serum level. The percentage of memory T cells had a positive association with IFN-γ serum level.
The Effect of Aerobic Exercise and Caloric Restriction on Mice’s Brain Tissue PGC-1α Levels and Their Memory Abilities
Authors: Irfannuddin Irfannuddin , Santoso Budi , Hidayati Zein Renny , Faris Naufal Adnan 
Published: July 2019

Abstract
Brain degeneration due to oxidative stress will have an impact on cognitive function decline. Aerobic exercise and calorie restriction are equally believed to increase the ability of cells to inhibit the effects of oxidative stress in the brain. Mitochondria are very susceptible to damage due to oxidative stress. Survival ability of mitochondria can be seen by measuring the levels of the PGC-1α protein, which regulates the transcription of mitochondrial biogenesis in brain tissue. A study has been conducted to compare the effects of aerobic exercise and calorie restriction for 8 weeks on PGC-1α levels of brain tissue of mice and their spatial memory. A total of 24 male mice, aged 6 weeks, were divided into 4 groups: control, aerobic exercise (AE), calorie restriction (CR), and a combination of AE + CR. The exercise was done by running in running wheels, every day for 40 minutes at a speed of 10 m / min. Calorie restriction was done by feeding mice as much as 70% of the calories of food consumed by ad libitum. The CR group had a PGC-1a levels and spatial memory higher than the control. The AE group and the combination of AE + CR had PGC-1a levels and spatial memory higher than the CR group, and both groups have no differences. Physical exercise accompanied with calorie restriction or only physical exercise have better effects on brain’s PGC-1a and spatial memory compared with only caloric restriction.
Number of foetus in pregnant Mus musculus which was injected by anti Qa2 and given mild regular exercise: endothelial dysfunction animal model to induce preeclampsia
Authors: Noor MS. , Santoso B. , Triawanti  , Widjiati  
Published: July 2019

Abstract
Preeclampsia community guidline (PRECOG) defined preeclampsia as a condition which was identified by the diastol blood pressure ≥ 90 mmHg and proteinuria in ≥ 20 weeks of pregnant. Basic mechanism of preeclampsia was endothelial dysfunction. One of preeclampsia’s impacts was intra uterine fetal death. It could be signed by less number of foetus. One of ways to prevent preeclampsia’s process was mild regular exercise. This research’s goal was analyzing the effect of mild regular exercise to number of foetus in pregnant Mus musculus which was injected by anti QA2 as endothelial dysfunction animal model to induce preeclampsia. The design was experimental. It used 6 Mus musculus/group. The groups were control (normal pregnant/K1), pregnant Mus musculus which was injected by anti QA2 (endithelial dysfunction model/K2), pregnant Mus musculus which was injected by anti QA2 and given mild regular exercise since early pregnant (K3), and pregnant Mus musculus which was injected by anti QA2 and given mild regular exercise since 1 week before pregnant (K4). Statistical analyze used Kruskal Wallis Test (α=0,05). It showed there was no significant different of foetus’ number among all groups. The conclussion was there was no effect of mild regular exercise to number of foetus in pregnant Mus musculus which was injected by anti QA2 as endothelial dysfunction animal model to induce preeclampsia.
Association between size and type of ventricular septal defect and nutritional status in children
Authors: Nova Ria , Salverra Yosy Deny 
Published: July 2019

Abstract
Ventricular septal defect (VSD) is often associated with malnutrition. Nutritional status in VSD is affected by inadequate energy intake or increase energy requirement. The objective of this study to assess the association between size and type of VSD and nutritional status. A cross-sectional study was conducted at Dr. Mohammad Hoesin Hospital Palembang. Data were collected from medical record and statistical analysis Chi square. All of 401 VSD patients were 68.1% perimembranous outlet, 25.7% doubly committed subarterial, 2.7% perimembranous outlet extension to inlet, 2.5% muscular and 1% inlet. The size of VSD were 133 (33.2%) small, 219 (54.6%) moderate, 49 (12.2%) large. There were 43 (10.7%) severely underweight, 278 (69.3%) underweight, 80 (20%) well nourished; 41 (10.2%) stunting, 360 (72%) normal height; 18 (4.5%) severely wasting, 242(60.3%) wasting and 141 (35.2%) well-nourished based on WAZ, HAZ and WHZ measurement respectively. There were a significant association between size of VSD and underweight (OR 18.147; 95% CI 9.47 to 34.76; p=0.00) and wasting (OR 25.09; 95% CI 14.55 to 43.26; p=0.00). There was a significant association between type of VSD and stunting (p=0.02). Our conclusion that significant association between size, underweight and wasting, also between type and stunting.
Preanesthetic maternal anxiety of 0 – 12 years old children who will undergo anesthesia and the contributing factors
Authors: Perdana Aries , Listyo Astuti Sarah , Ade Wijaya Ramlan Andi , Farida Soenarto Ratna , Wiguna Tjhin 
Published: July 2019

Abstract
Emotional stress, such as seen in preanesthetic anxiety, often occurs in parents and children who will undergo surgery and anesthesia. Preanesthetic anxiety is a subjective feeling associated with increased autonomic nervous system activity. Preanesthetic anxiety experienced by parents can be passed onto their children and have a negative impact on a child such as the occurrence of post anesthesia behaviour disorders. In Indonesia, data about preanesthetic maternal anxiety is still unavailable. The purpose of this study is to determine the proportion of preanesthetic maternal anxiety and the contributing factors. After obtaining ethical approval from Health Research Ethics Committee of FKUI-RSCM, 144 mothers of children aged 0 – 12 years at preoperative clinic Dr. Cipto Mangunkusumo Hospital who were scheduled to undergo anesthesia, were interviewed using an anxiety disorders interview guide MINI (Mini International Neuropsychiatric Interview) ICD-10. The anxiety scores were assessed using the VAS (Visual Analogue Scale). Results were analyzed using multivariate logistic regression. A total of 70 mothers (48,6%) had anxiety disorders with VAS score ranging between 3- 8 mm. Preanesthetic maternal anxiety is influenced by the child’s age (p = 0.001), history of previous anesthesia on the child (p = 0.004), and number of children (p = 0.041). Mothers with infants are 7.9 times more likely to experience higher anxiety (adjusted OR = 7.982), but because of the wide range of confidence interval (95% CI = 2.599 to 24.512) other factors may also influence the preanesthetic maternal anxiety. Preanesthetic maternal anxiety has fairly high proportion in numbers. This problem needs to be addressed to prevent a negative impact on children post anesthesia.
Effects of Neoadjuvant FAC Chemotherapy response to CD4 + Serum Levels before and after chemotherapy in breast cancer patients local advanced stage at Dr. Mohammad Hoesin Palembang General Hospital
Authors: Permana Ade , Kusuma Benny , Qodir Nur , Bahar Erial 
Published: July 2019

Abstract
CD4+ T helper has an important role in maintain long-term antitumor effect. AJ madu reported that there was a significant decrease between CD4 values before and after chemotherapy. Researchers wanted to investigate the effects of neoadjuvant chemotherapy on CD4+ levels in patients with locally advanced breast cancer at Dr. Mohammad Hoesin Palembang. This study was a non-comparable clinical trial by looking at serum CD4+ levels in locally advanced breast cancer patients before and after neoadjuvant chemotherapy. There are 30 subjects the subject age ranged from 33-66 years with an average of 45 years. 29 patients with marital status (96.7%), 17 patients with contraception history (56.7%), 13 patients with family history of Breast Cancer (43.3%). 23 patients with good chemotherapy response (76.7%) and 7 patients who had poor chemotherapy response (23.3%). Statistical test results using paired t-test showed that there was a significant difference in mean CD4+ count before and after neoadjuvan chemotherapy. At the CD4+ level before chemotherapy 775.55 had a sensitivity of 60% and a specificity of 57% (cut of point). While CD4+ levels after chemotherapy 470.85 with sensitivity of 60% and specificity of 57%. CD4+ pre-chemotherapy examination had a sensitivity score of 60% and a specificity of 57%.
Diversity and abundance model according to habitat characteristics of filariasis vector, Mansonia spp. in Banyuasin, South Sumatera, Indonesia
Authors: Pratiwi Rini , Anwar Chairil , Salni  , Hermansyah  , Novrikasari  , Hidayat Rachmat , Ghiffari Ahmad , Alfarobi  
Published: July 2019

Abstract
Filariasis is an infectious disease caused by filariae worm transmitted by a mosquito as vectors. Mansonia is one of the genera of filariasis vectors. The high risk of filariasis transmission is the existence of habitat character which is qualified for Mansonia spp. One of filariasis endemic area is Banyuasin, South Sumatera, as much as 2.02% microfilaria rate. This study was conducted to estimate the number of vector abundance influenced by habitat characteristic. The data collection was from two villages: Sedang and Muara Sugih, from April 2017 – May 2018. Mosquitos were caught using aspirators and humans as baits. Ecological factors were the physical environment such as temperature measured by thermometer and humidity measured by sling hygrometer. Water parameter and geographic factors were analyzed. Abundance model of Mansonia spp described using regression test for multivariate analysis. Data analysis was performed and presented in form of frequency tables. The number of samples was 4.956 mosquitoes. There were six species such as Mansonia uniformis, Mansonia annulifera, Mansonia indiana, Mansonia dives, Mansonia bonneae, and Mansonia annulata. Sedang Village was dominated by Mansonia uniformis (41.25%) whereas, Muara Sugih Village was by Mansonia bonneae (45.66%). The ideal habitat characteristics for Mansonia spp were environmental conditions with high temperature and humidity, low water turbidity, low water temperature, low pH, TDS, and high TSS. The diversity and abundance of Mansonia spp as a potential vector of filariasis Brugia malayi in Banyuasin regency was closely related to environmental characteristics such as temperature, humidity, water parameters, and plant species.
Kidney protective effect of acalypha indica linn. root extract in high-fructose and high-cholesterol diet-fed rats
Authors: Nielya Mutiara Putri Anyelir , Wardani Hakim Rani , Purwaningsih Erni H. , Gede Budi Krisnamurti Desak 
Published: July 2019

Abstract
Diet high in fructose and cholesterol may lead to the development of diabetic nephropathy (DN). One of the first drugs of choice in DN treatment is captopril. Prolonged use of this drug may lead to some adverse effects and the treatment can be optimized through using other therapy options. Acalypha indica Linn. (AI) may be an alternative herbal therapy for DN. The objective of this research is to investigate the renoprotective effect of AI on DN. For seven weeks, thirty-two Sprague-Dawley rats were divided into groups receiving normal diet and high-fructose and high-cholesterol diet (HFCD). Then, the HFCD-fed rats were divided into four groups receiving different treatment: negative control, AI root extract (250 mg/kgBW), captopril (2.5 mg/kgBW), and combination of captopril and AI. Normal diet group was divided into AI and no treatment. After four weeks of treatment, the rats were terminated and serum urea and creatinine levels were measured. In the normal group, AI therapy decreased serum urea and creatinine levels. In the HFCD groups, AI and captopril monotherapy groups had increased serum urea levels, but lower compared to negative control. Meanwhile, serum creatinine levels decreased in both groups. However, these findings are not statistically significant. We found that combination therapy group had the highest increase in serum urea level, which was significantly different with captopril group (p=0.01). Serum creatinine level was also increased in this group. Our present study showed that AI tend to reduce serum urea and creatinine levels in normal diet group and inhibit the increase of serum urea and creatinine levels in rats fed with HFCD diet. Antagonistic interaction between captopril and AI might be present.
Identification of blaIMP gene carbapenem-resistant Enterobacteriaceae (CRE) isolated from patient infection
Authors: Sabrina Tia , Amalia Ella , Patricia Venny , Yuwono  , Usman Rahmat M. 
Published: July 2019

Abstract
There have been increasing cases of carbapenem-resistant Enterobacteriaceae (CRE) through the production of enzymes carbapenemase in the last decade. BlaIMP
gene acts as a gene encoding the enzyme production carbapenemase that hydrolyzes beta-lactam ring in carbapenem antibiotics. The mechanism of bacterial plasmid transfer among Enterobacteriaceae facilitates the spread of carbapenem antibiotic resistance to other bacterial species. This study aims to identify the genes blaIMP
in carbapenem-resistant Enterobacteriaceae at Dr. Mohammad Hoesin Hospital. This research is an observational research laboratory. The population of Enterobacteriaceae bacteria is accumulated in clinical microbiology laboratories RSMH, period September-November 2017, then performed a test to get a sample of bacterial phenotype CRE through Vitex 2® compact bioMerieux tool. CRE phenotype sample obtained bacteria, later identified to determine genotype blaIMP
CRE bacteria samples by PCR and visualized on a 232 bp band. A total of 35 from 730 Enterobacteriaceae bacteria population (4.8%) was identified as bacterial phenotype CRE. A total of three from 35 samples of the CRE phenotype (8.6%) was identified containing the gene genotype blaIMP
. The third came from sputum samples containing Klebsiella pneumoniae, pus containing Enterobacter cloacae and blood containing Serratia marcescens. In this study identified three samples (8.6%) bacterial genes phenotype blaIMP
CRE.