bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cross-resistance to acetolactate synthase (ALS) inhibitors associated with different mutations in Japanese foxtail (Alopecurus japonicus)
Authors: Zhao Ning , Bi Yaling , Wu Cuixia , Wang Dandan , You Ludan , Liu Weitang , Wang Jinxin 
Published: May 2019

AbstractJapanese foxtail (Alopecurus japonicus Steud.) is an invasive grass weed that severely threatens the production of wheat (Triticum aestivum L.) and canola (Brassica napus L.) crops in eastern Asia. Mesosulfuron-methyl is a highly efficient acetolactate synthase (ALS)-inhibiting herbicide widely used for control of this species in China. However, in recent years, some A. japonicus populations have evolved resistance to mesosulfuron-methyl by different amino acid substitutions (AASs) within the ALS gene. In the current study, 11 populations of A. japonicus were collected from Anhui Province, China, where the wheat fields were severely infested with this weed. Based on single-dose screening, eight of these populations evolved resistance to mesosulfuron-methyl, and gene sequencing revealed three AASs located in codon 197 or 574 of the ALS gene in the different resistant populations. Subsequently, three typical populations, AH-1, AH-4, and AH-10 with Trp-574-Leu, Pro-197-Thr, and Pro-197-Ser mutations, respectively, in ALS genes were selected to characterize their cross-resistance patterns to ALS inhibitors. Compared with the susceptible population AH-S, AH-1 showed broad-spectrum cross-resistance to sulfonylureas (SUs), imidazolinones (IMIs), triazolopyrimidines (TPs), and sulfonyl-aminocarbonyl-triazolinones (SCTs); whereas AH-4 and AH-10 were resistant to SUs, TPs, and SCTs but sensitive to IMIs. Moreover, all three resistant populations were sensitive to both photosystem II inhibitor isoproturon and 4-hydroxyphenylpyruvate dioxygenase inhibitor QYM201 (1-(2-chloro-3-(3-cyclopropyl-5-hydroxy-1-methyl-1H-pyrazole-4-carbonyl)-6-(trifluoromethyl)phenyl)piperidin-2-one). Based on the current state of knowledge, this study is the first report of A. japonicus evolving cross-resistance to ALS-inhibiting herbicides due to a Pro-197-Ser mutation in the ALS gene.
Melt‐Induced Fractionation of Major Ions and Trace Elements in an Alpine Snowpack
Authors: Avak Sven E. , Trachsel Jürg C. , Edebeli Jacinta , Brütsch Sabina , Bartels‐Rausch Thorsten , Schneebeli Martin , Schwikowski Margit , Eichler Anja 
Published: May 2019

AbstractUnderstanding the impact of melting on the preservation of atmospheric compounds in high‐Alpine snow and glacier ice is crucial for future reconstruction of past atmospheric conditions. However, detailed studies investigating melt‐related changes of such proxy information are rare. Here we present a series of five snow pit profiles of 6 major ions and 34 trace elements at Weissfluhjoch, Switzerland, collected between January and June 2017. Atmospheric composition was preserved during the cold season, while melting toward the summer resulted in preferential loss of certain species from the snowpack or enrichment at the base of the snowpack. Increasing mobilization of major ions with meltwater (NH4+ < Cl− ~ Na+ < NO3− ~ Ca2+ ~ SO42−) can be related to their stronger enrichment at ice crystal surfaces during snow metamorphism. Results for trace elements show that less abundant elements such as Ce, Eu, La, Mo, Nd, Pb, Pr, Sb, Sc, Sm, U, and W were best preserved and may still serve as tracers to reconstruct past natural and anthropogenic atmospheric emissions from melt‐affected snow pit and ice core records. The obtained elution behavior matches the findings from another high‐Alpine site (upper Grenzgletscher) for major ions and the large majority of investigated trace elements. Both studies indicate that water solubility and location at the microscopic scale are likely to determine the relocation behavior with meltwater and also suggest that the observed species‐dependent preservation from melting snow and ice is representative for the Alpine region, reflecting Central European atmospheric aerosol composition.
Identification of genomic region(s) responsible for high iron and zinc content in rice
Authors: Dixit Shilpi , Singh Uma Maheshwar , Abbai Ragavendran , Ram T. , Singh Vikas Kumar , Paul Amitava , Virk P. S. , Kumar Arvind 
Published: May 2019

AbstractMicronutrient especially iron and zinc-enriched rice hold immense promise for sustainable and cost-effective solutions to overcome malnutrition. In this context, BC2F5 population derived from cross between RP-Bio226 and Sampada was used to localize genomic region(s)/QTL(s) for grain Fe (iron) and Zn (zinc) content together with yield and yield-related traits. Genotyping of mapping population with 108 SSR markers resulted in a genetic map of 2317.5 cM with an average marker distance of 21.5 cM. Mean grain mineral content in the mapping population across the two seasons ranged from 10.5–17.5 ppm for Fe and 11.3–22.1 ppm for Zn. Based on the multi-season phenotypic data together with genotypic data, a total of two major QTLs for Fe (PVE upto 17.1%) and three for Zn (PVE upto 34.2%) were identified. Comparative analysis across the two seasons has revealed four consistent QTLs for Fe (qFe1.1, qFe1.2, qFe6.1 and qFe6.2) and two QTL for Zn content (qZn1.1 and qZn6.2). Additionally, based on the previous and current studies three meta-QTLs for grain Fe and two for grain Zn have been identified. In-silico analysis of the identified QTL regions revealed the presence of potential candidate gene(s) such as, OsPOT, OsZIP4, OsFDR3, OsIAA5 etc., that were previously reported to influence grain Fe and Zn content. The identified QTLs could be utilized in developing high yielding, Fe and Zn denser varieties by marker assisted selection (MAS).
Impaired memory-guided attention in asymptomatic APOE4 carriers
Authors: Zimmermann Jacqueline , Alain Claude , Butler Chris 
Published: May 2019

AbstractAttention and memory may be impaired in individuals at-risk for Alzheimer’s disease (AD), though standard cognitive assessments typically study the two in parallel. In reality, attention and memory interact to facilitate information processing, and thus a more integrative approach is required. Here, we used a novel auditory paradigm to assess how long-term memory for auditory scenes facilitates detection of an auditory target in asymptomatic carriers of Apolipoprotein E4 (APOE4), the principle risk gene for late-onset AD. We tested 60 healthy middle-aged adults with varying doses of APOE4 - 20 APOE4 homozygotes (E4/E4), 20 heterozygotes (E3/E4) and 20 non-carriers (E3/E3) - to determine effect on memory-guided attention. While explicit memory was unaffected by genotype, APOE4 dose significantly impaired memory-guided attention. A relationship between explicit memory and memory-guided attention was observed in non-carriers, but this correlation was not significant in E3/E4 and E4/E4 carriers, suggesting that APOE4 carriers rely less on explicit memory to facilitate attention. Since memory-guided attention declined with age in APOE4 homozygotes, this impairment may reflect early disease rather than being a life-long trait. In sum, asymptomatic individuals at increased genetic risk of AD show an age-dependent decline in attention-memory interaction when memory alone is not impaired.
Physical Radiation Enhancement Effects Around Clinically Relevant Clusters of Nanoagents in Biological Systems
Authors: Villagomez-Bernabe B. , Currell F. J. 
Published: May 2019

AbstractHere we show that the determining factor for physical radiation enhancement effects for a clinically realistic cluster of heavy-atom bearing nanoparticles is the total number of heavy atoms packed into the cluster. We do this through a multiscale Monte Carlo approach which permits the consideration of radiation transport through clusters of millions of nanoparticles. The finding is in contrast to that predicted when isolated nanoparticles are considered and is a direct consequence of the Auger electrons playing less of a role for clusters compared to isolate nanoparticles. We further show that this result is agnostic to selection of the subcellular region considered to be sensitive to the effects of radiation, provided the inside the cluster of nanoparticles is not considered to be biologically active.
Development of a DNA isolation device using poly(3,4-dihydroxy-L-phenylalanine)-coated swab for on-site molecular diagnostics
Authors: Park Hyun-Ju , Cho Heesoo , Jung Ho Sang , Cho Baek Hwan , Lee Min-Young 
Published: May 2019

AbstractFor on-site molecular diagnostics, a pre-treatment step for isolation of nucleic acid from clinical samples on site is desired. However, conventional commercialized silica-based nucleic acid isolation kits require repetitive pipetting and a centrifugation or permanent magnet for buffer exchange. In this study, we developed a poly(3,4-dihydroxy-L-phenylalanine) (L-DOPA)-coated swab that can absorb and desorb DNA depending on pH of buffers and a portable integrated DNA isolation device that comprises integrated chambers containing DNA isolation buffers. The poly(L-DOPA)-coated swab interacts with each buffer by passing through the membrane between the integrated chambers. Our device involves a simple operation and does not require any large equipment or skilled experts. By connecting the device with an automated polymerase chain reaction system, an isothermal amplification system, or a non-amplified DNA detection method, on-site molecular diagnosis of various diseases can be quickly realized.
Plasmodium pseudo-Tyrosine Kinase-like binds PP1 and SERA5 and is exported to host erythrocytes
Authors: Gnangnon Bénédicte , Fréville Aline , Cailliau Katia , Leroy Catherine , De Witte Caroline , Tulasne David , Martoriarti Alain , Jung Vincent , Guerrera Ida Chiara , Marion Sabrina , Khalife Jamal , Pierrot Christine 
Published: May 2019

AbstractPseudokinases play key roles in many biological processes but they are poorly understood compared to active kinases. Eight putative pseudokinases have been predicted in Plasmodium species. We selected the unique pseudokinase belonging to tyrosine kinase like (TKL) family for detailed structural and functional analysis in P. falciparum and P. berghei. The primary structure of PfpTKL lacks residues critical for kinase activity, supporting its annotation as a pseudokinase. The recombinant pTKL pseudokinase domain was able to bind ATP, but lacked catalytic activity as predicted. The sterile alpha motif (SAM) and RVxF motifs of PfpTKL were found to interact with the P. falciparum proteins serine repeat antigen 5 (SERA5) and protein phosphatase type 1 (PP1) respectively, suggesting that pTKL has a scaffolding role. Furthermore, we found that PP1c activity in a heterologous model was modulated in an RVxF-dependent manner. During the trophozoite stages, PbpTKL was exported to infected erythrocytes where it formed complexes with proteins involved in cytoskeletal organization or host cell maturation and homeostasis. Finally, genetic analysis demonstrated that viable strains obtained by genomic deletion or knocking down PbpTKL did not affect the course of parasite intra-erythrocytic development or gametocyte emergence, indicating functional redundancy during these parasite stages.
Differing responses in milk composition from introducing rapeseed and naked oats to conventional and organic dairy diets
Authors: Butler Gillian , Stergiadis Sokratis , Chatzidimitriou Eleni , Franceschin Enrica , Davis Hannah R. , Leifert Carlo , Steinshamn Håvard 
Published: May 2019

AbstractDairy products are often considered challenging for health due to their saturated fatty acid content, yet they also provide beneficial nutrients, some unique to ruminants. The degree of fat saturation is influenced by cows’ diets; grazing pasture enhances unsaturated fatty acids in milk compared with conserved forages. These benefits can be partially mimicked by feeding oilseeds and here we consider the impact on milk composition in a 2 × 2 trial, feeding rapeseed to both conventional and organic cows, finding very differing lipid metabolism in the 4 experimental groups. For milk fat, benefits of organic rather than conventional management (+39% PUFA, +24% long chain omega-3 and +12% conjugated linoleic acid (CLA)) appear complementary to those from feeding rape (+43% MUFA, +10% PUFA, +40% CLA), combining to produce milk 16% lower SFA and higher in MUFA (43%), PUFA (55%) and CLA (59%). Organic and rape feeding provide less omega-3 PUFA than the conventional and control diets, yet contrary to expectations, together they almost doubled (+94%) the omega-3 concentration in milk, implying a 3.8 fold increase in net transfer from diet into milk. Organic and rape feeding also gave lower trace-elements and antioxidants in milk. Greater understanding of these phenomena might enhance the sustainability of dairying.
Human mid-trimester amniotic fluid (stem) cells lack expression of the pluripotency marker OCT4A
Authors: Vlahova Filipa , Hawkins Kate E. , Ranzoni Anna Maria , Hau Kwan-Leong , Sagar Rachel , De Coppi Paolo , David Anna L. , Adjaye James , Guillot Pascale V. 
Published: May 2019

AbstractExpression of OCT4A is one of the hallmarks of pluripotency, defined as a stem cell’s ability to differentiate into all the lineages of the three germ layers. Despite being defined as non-tumorigenic cells with high translational potential, human mid-trimester amniotic fluid stem cells (hAFSCs) are often described as sharing features with embryonic stem cells, including the expression of OCT4A, which could hinder their clinical potential. To clarify the OCT4A status of hAFSCs, we first undertook a systematic review of the literature. We then performed extensive gene and protein expression analyses to discover that neither frozen, nor fresh hAFSCs cultivated in multipotent stem cell culture conditions expressed OCT4A, and that the OCT4A positive results from the literature are likely to be attributed to the expression of pseudogenes or other OCT4 variants. To address this issue, we provide a robust protocol for the assessment of OCT4A in other stem cells.
Laboratory study of stationary accretion shock relevant to astrophysical systems
Authors: Mabey P. , Albertazzi B. , Falize E. , Michel Th. , Rigon G. , Van Box Som L. , Pelka A. , Brack F.-E. , Kroll F. , Filippov E. , Gregori G. , Kuramitsu Y. , Lamb D. Q. , Li C. , Ozaki N. , Pikuz S. , Sakawa Y. , Tzeferacos P. , Koenig M. 
Published: May 2019

AbstractAccretion processes play a crucial role in a wide variety of astrophysical systems. Of particular interest are magnetic cataclysmic variables, where, plasma flow is directed along the star’s magnetic field lines onto its poles. A stationary shock is formed, several hundred kilometres above the stellar surface; a distance far too small to be resolved with today’s telescopes. Here, we report the results of an analogous laboratory experiment which recreates this astrophysical system. The dynamics of the laboratory system are strongly influenced by the interplay of material, thermal, magnetic and radiative effects, allowing a steady shock to form at a constant distance from a stationary obstacle. Our results demonstrate that a significant amount of plasma is ejected in the lateral direction; a phenomenon that is under-estimated in typical magnetohydrodynamic simulations and often neglected in astrophysical models. This changes the properties of the post-shock region considerably and has important implications for many astrophysical studies.
Response of an actin network in vesicles under electric pulses
Authors: Perrier Dayinta L. , Vahid Afshin , Kathavi Vaishnavi , Stam Lotte , Rems Lea , Mulla Yuval , Muralidharan Aswin , Koenderink Gijsje H. , Kreutzer Michiel T. , Boukany Pouyan E. 
Published: May 2019

AbstractWe study the role of a biomimetic actin network during the application of electric pulses that induce electroporation or electropermeabilization, using giant unilamellar vesicles (GUVs) as a model system. The actin cortex, a subjacently attached interconnected network of actin filaments, regulates the shape and mechanical properties of the plasma membrane of mammalian cells, and is a major factor influencing the mechanical response of the cell to external physical cues. We demonstrate that the presence of an actin shell inhibits the formation of macropores in the electroporated GUVs. Additionally, experiments on the uptake of dye molecules after electroporation show that the actin network slows down the resealing process of the permeabilized membrane. We further analyze the stability of the actin network inside the GUVs exposed to high electric pulses. We find disruption of the actin layer that is likely due to the electrophoretic forces acting on the actin filaments during the permeabilization of the GUVs. Our findings on the GUVs containing a biomimetic network provide a step towards understanding the discrepancies between the electroporation mechanism of a living cell and its simplified model of the empty GUV.
New type of doping effect via metallization of surface reduction in SnO2
Authors: Bang Jae Hoon , Choi Myung Sik , Na Han Gil , Oum Wansik , Choi Sun-Woo , Kim Sang Sub , Kim Hyoun Woo , Jin Changhyun 
Published: May 2019

AbstractThe use of conventional doping methods requires consideration of not only the energy connection with the base material but also the limits of the type and doping range of the dopant. The scope of the physico-chemical change must be determined from the properties of the base material, and when this limit is exceeded, a large energy barrier must be formed between the base material and the dopant as in a heterojunction. Thus, starting from a different viewpoint, we introduce a so-called metallization of surface reduction method, which easily overcomes the disadvantages of existing methods while having the effect of doping the base material. Such new synthetic techniques enable sequential energy arrangements–gradients from the surface to the centre of the material–so that free energy transfer effects can be obtained as per the energies in the semiconducting band, eliminating the energy discontinuity of the heterojunction.
Post-diagnostic C-reactive protein and albumin predict survival in Chinese patients with non-small cell lung cancer: a prospective cohort study
Authors: Yang Jin-Rong , Xu Jia-Ying , Chen Guo-Chong , Yu Na , Yang Jing , Zeng Da-Xiong , Gu Min-Jing , Li Da-Peng , Zhang Yu-Song , Qin Li-Qiang 
Published: May 2019

AbstractNon-small cell lung cancer (NSCLC) is the most commonly diagnosed lung cancer and is associated with poor prognosis. This study aimed to analyze if serum C-reactive protein (CRP), albumin (Alb), and CRP/Alb ratio could provide prognostic information in patients with NSCLC. 387 patients with primary NSCLC were included in this analysis. Cox proportional hazards regression was used to estimate hazard ratio (HR) and 95% confidence interval (CI) of death with adjustment for some potential confounders. The multivariate regression analyses revealed the statistically significant associations of decreased survival of patients with NSCLC with elevated CRP, decreased Alb, and elevated CRP/Alb ratio. The HRs of mortality were 1.56 (95% CI: 0.80–3.04) and 2.64 (95% CI: 1.35–5.16) for patients in the second and the highest tertiles of CRP (P-trend = 0.003). For albumin, the HR was 0.50 (95% CI: 0.29–0.85) for the normal group. The CRP/Alb ratio strongly predicted the survival of patients in the highest tertile with a fourfold risk of dying compared with those in the lowest tertile (HR = 4.14, 95% CI: 2.15–7.98). The subgroup analysis according to various patient characteristics confirmed these associations. In conclusion, serum CRP, albumin, and CRP/Alb ratio are predictive of survival for Chinese patients with NSCLC.
Detection of alveolar bone defects with three different voxel sizes of cone-beam computed tomography: an in vitro study
Authors: Dong Ting , Yuan Lingjun , Liu Lu , Qian Yifeng , Xia Lunguo , Ye Niansong , Fang Bing 
Published: May 2019

AbstractThis study was conducted to assess the accuracy of cone-beam computed tomography (CBCT) of different voxel sizes in the detection of alveolar bone defects, and to select the optimal voxel size for clinical use. 46 in-vitro teeth were placed in bovine ribs in which alveolar bone defects were randomly simulated. In total, 32 alveolar bone defects and 14 teeth without periodontal defects were used. CBCT images were acquired with the use of three different voxel sizes: 0.125-mm, 0.2-mm and 0.4-mm. The scan data were 3D-reconstructed in Mimics software and evaluated by two observers with more than 5 years of experience in CBCT. Receiver operating characteristic (ROC) curves and diagnostic values were obtained. Pairwise comparison of ROC curves was made for evaluation of the diagnostic values of different voxel sizes. Kappa statistics assessed the observer reliability. Results were considered significant at P < 0.05. It showed no statistically significant difference between 0.125-mm group and 0.2-mm group, but 0.4-mm group had lower Az values that differed significantly from 0.125-mm and 0.2-mm groups (P < 0.05). Based on diagnostic value and radiation protection, 0.2-mm voxel size may be a good choice for the detection of bone defects with CBCT.
The Close AGN Reference Survey (CARS)
Authors: Husemann B. , Scharwächter J. , Davis T. A. , Pérez-Torres M. , Smirnova-Pinchukova I. , Tremblay G. R. , Krumpe M. , Combes F. , Baum S. A. , Busch G. , Connor T. , Croom S. M. , Gaspari M. , Kraft R. P. , O’Dea C. P. , Powell M. , Singha M. , Urrutia T. 
Published: May 2019

Context. Galaxy-wide outflows driven by star formation and/or an active galactic nucleus (AGN) are thought to play a crucial rule in the evolution of galaxies and the metal enrichment of the inter-galactic medium. Direct measurements of these processes are still scarce and new observations are needed to reveal the nature of outflows in the majority of the galaxy population.Aims. We combine extensive, spatially-resolved, multi-wavelength observations, taken as part of the Close AGN Reference Survey (CARS), for the edge-on disc galaxy HE 1353−1917 in order to characterise the impact of the AGN on its host galaxy via outflows and radiation.Methods. Multi-color broad-band photometry was combined with spatially-resolved optical, near-infrared (NIR) and sub-mm and radio observations taken with the Multi-Unit Spectroscopy Explorer (MUSE), the Near-infrared Integral Field Spectrometer (NIFS), the Atacama Large Millimeter Array (ALMA), and theKarl G. JanskyVery Large Array (VLA) to map the physical properties and kinematics of the multi-phase interstellar medium.Results. We detect a biconical extended narrow-line region ionised by the luminous AGN orientated nearly parallel to the galaxy disc, extending out to at least 25 kpc. The extra-planar gas originates from galactic fountains initiated by star formation processes in the disc, rather than an AGN outflow, as shown by the kinematics and the metallicity of the gas. Nevertheless, a fast, multi-phase, AGN-driven outflow with speeds up to 1000 km s−1is detected close to the nucleus at 1 kpc distance. A radio jet, in connection with the AGN radiation field, is likely responsible for driving the outflow as confirmed by the energetics and the spatial alignment of the jet and multi-phase outflow. Evidence for negative AGN feedback suppressing the star formation rate (SFR) is mild and restricted to the central kpc. But while any SFR suppression must have happened recently, the outflow has the potential to greatly impact the future evolution of the galaxy disc due to its geometrical orientation.Conclusions.. Our observations reveal that low-power radio jets can play a major role in driving fast, multi-phase, galaxy-scale outflows even in radio-quiet AGN. Since the outflow energetics for HE 1353−1917 are consistent with literature, scaling relation of AGN-driven outflows the contribution of radio jets as the driving mechanisms still needs to be systematically explored.