bluuebunny/crossref_metadata_2025_split huggingface dataset:
Protein Tyrosine Phosphatase 4A3 (PTP4A3) modulates Src signaling in T-cell Acute Lymphoblastic Leukemia to promote leukemia migration and progression
Authors: Wei M , Haney MG , Blackburn JS 
Published: June 2019

AbstractT-cell Acute Lymphoblastic Leukemia (T-ALL) is an aggressive blood cancer, and currently, there are no immunotherapies or molecularly targeted therapeutics available for treatment of this malignancy. The identification and characterization of genes and pathways that drive T-ALL progression is critical for development of new therapies for T-ALL. Here, we determined that Protein Tyrosine Phosphatase 4A3 (PTP4A3) plays a critical role in disease initiation and progression by promoting cell migration in T-ALL. PTP4A3 expression was upregulated in patient T-ALL samples at both the mRNA and protein levels compared to normal lymphocytes. Inhibition of PTP4A3 function with a small molecule inhibitor and knock-down of PTP4A3 expression using short-hairpin RNA (shRNA) in human T-ALL cells significantly impeded T-ALL cell migration capacityin vitroand reduced their ability to engraft and proliferatein vivoin xenograft mouse models. Additionally, PTP4A3 overexpression in aMyc-induced zebrafish T-ALL model significantly accelerated disease onset and shortened the time needed for cells to enter blood circulation. Reverse phase protein array (RPPA) revealed that manipulation of PTP4A3 expression levels in T-ALL cells directly affected the SRC signaling pathway, which plays a well-characterized role in migratory behavior of several cell types. Taken together, our study revealed that PTP4A3 is a key regulator of T-ALL migration via SRC signaling, and suggests that PTP4A3 plays an important role as an oncogenic driver in T-ALL.HighlightsA subset of T-cell Acute Lymphoblastic Leukemia (T-ALL) highly express the phosphatase PTP4A3PTP4A3 expression promotes leukemia development in zebrafish T-ALL modelsLoss of PTP4A3 prevents T-ALL engraftment in mouse xenograft modelsKnock-down or small molecule inhibition of PTP4A3 prevents T-ALL migration in part via modulation of SRC signaling.
On-demand, cell-free biomanufacturing of conjugate vaccines at the point-of-care
Authors: Stark Jessica C. , Jaroentomeechai Thapakorn , Moeller Tyler D. , Dubner Rachel S. , Hsu Karen J. , Stevenson Taylor C. , DeLisa Matthew P. , Jewett Michael C. 
Published: June 2019

SummaryConjugate vaccines are among the most effective methods for preventing bacterial infections, representing a promising strategy to combat drug-resistant pathogens. However, existing manufacturing approaches limit access to conjugate vaccines due to centralized production and cold chain distribution requirements. To address these limitations, we developed a modular technology forin vitrobioconjugatevaccine expression (iVAX) in portable, freeze-dried lysates from detoxified, nonpathogenicEscherichia coli. Upon rehydration, iVAX reactions synthesize clinically relevant doses of bioconjugate vaccines against diverse bacterial pathogens in one hour. We show that iVAX synthesized vaccines against the highly virulent pathogenFranciscella tularensissubsp.tularensis(type A) strain Schu S4 elicited pathogen-specific antibodies in mice at significantly higher levels compared to vaccines produced using engineered bacteria. The iVAX platform promises to accelerate development of new bioconjugate vaccines with increased access through refrigeration-independent distribution and point-of-care production.
Illusory sound texture reveals multi-second statistical completion in auditory scene analysis
Authors: McWalter Richard , McDermott Josh H. 
Published: June 2019

AbstractSound sources in the world are experienced as stable even when intermittently obscured, implying perceptual completion mechanisms that “fill in” missing sensory information. We demonstrate a filling-in phenomenon in which the brain extrapolates the statistics of background sounds (textures) over periods of several seconds when they are interrupted by another sound, producing vivid percepts of illusory texture. The effect differs from previously described completion effects in that 1) the extrapolated sound must be defined statistically given the stochastic nature of texture, and 2) in lasting much longer, enabling introspection and facilitating assessment of the underlying representation. Illusory texture appeared to be integrated into texture statistic estimates indistinguishably from actual texture, suggesting that it is represented similarly to actual texture. The illusion appears to represent an inference about whether the background is likely to continue during concurrent sounds, providing a stable representation of the environment despite unstable sensory evidence.
Chromatin accessibility dynamics ofChlamydia-infected epithelial cells
Authors: Hayward Regan J. , Marsh James W. , Humphrys Michael S. , Huston Wilhelmina M. , Myers Garry S.A. 
Published: June 2019

AbstractChlamydiaare Gram-negative, obligate intracellular bacterial pathogens responsible for a broad spectrum of human and animal diseases. In humans,Chlamydia trachomatisis the most prevalent bacterial sexually transmitted infection worldwide and is the causative agent of trachoma (infectious blindness) in disadvantaged populations. Over the course of its developmental cycle,Chlamydiaextensively remodels its intracellular niche and parasitises the host cell for nutrients, with substantial resulting changes to the host cell transcriptome and proteome. However, little information is available on the impact of chlamydial infection on the host cell epigenome and global gene regulation. Regions of open eukaryotic chromatin correspond to nucleosome-depleted regions, which in turn are associated with regulatory functions and transcription factor binding. We applied Formaldehyde-Assisted Isolation of Regulatory Elements enrichment followed by sequencing (FAIRE-Seq) to generate temporal chromatin maps ofC. trachomatis-infected human epithelial cellsin vitroover the chlamydial developmental cycle. We detected both conserved and distinct temporal changes to genome-wide chromatin accessibility associated withC. trachomatisinfection. The observed differentially accessible chromatin regions, including several Clusters of Open Regulatory Elements (COREs) and temporally-enriched sets of transcription factors, may help shape the host cell response to infection. These regions and motifs were linked to genomic features and genes associated with immune responses, re-direction of host cell nutrients, intracellular signaling, cell-cell adhesion, extracellular matrix, metabolism and apoptosis. This work provides another perspective to the complex response to chlamydial infection, and will inform further studies of transcriptional regulation and the epigenome inChlamydia-infected human cells and tissues
Insertion Preference of Maize and Rice Miniature Inverted Repeat Transposable Elements as Revealed by the Analysis of Nested Elements [W]
Authors: Jiang Ning , Wessler Susan R. 
Published: June 2019

AbstractA 128-bp insertion into the maize waxy-B2 allele led to the discovery of Tourist, a family of miniature inverted repeat transposable elements (MITEs). As a special category of nonautonomous elements, MITEs are distinguished by their high copy number, small size, and close association with plant genes. In maize, some Tourist elements (named Tourist-Zm) are present as adjacent or nested insertions. To determine whether the formation of multimers is a common feature of MITEs, we performed a more thorough survey, including an estimation of the proportion of multimers, with 30.2 Mb of publicly available rice genome sequence. Among the 6600 MITEs identified, &gt;10% were present as multimers. The proportion of multimers differs for different MITE families. For some MITE families, a high frequency of self-insertions was found. The fact that all 340 multimers are unique indicates that the multimers are not capable of further amplification.
Skill development research in India: a systematic literature review and future research agenda
Authors: Cabral Clement , Dhar Rajib Lochan 
Published: June 2019

Purpose
The purpose of this paper is to conduct an in-depth analysis of the literature and provide new insights on skill development research in India. Objectives in the study are twofold. First is to conduct descriptive analysis in terms of structural dimensions such as geographical context, focus on skill development, research methods, economic sector, type of training and origin of the study. The second objective is to conduct thematic analysis on importance of skill development, institutional and regulatory mechanisms, skill development as an antecedent for technology adoption, role of skill development in women empowerment, integrating skill development with secondary education and labour market reforms for skill development.

Design/methodology/approach
Systematic literature review method is employed to review 45 articles on skill development research in India conducted between 2004 and 2017.

Findings
The review discusses about the importance of skill development, the role of institutions such as NSDA and PMKVJ, skill development as a necessity in the advent of technological changes, an effective measure to empower women in the country and the need of integrating skill development with secondary education. Labour market reforms are required to overcome challenges such as mismatch between theory and practice, low quality of skills assimilated, the mismatch between demand and supply of skilled labour force, low level of in-house training, low cooperation from students and employees due to lack of incentives and lack of qualified teachers.

Originality/value
This study is the first to offer a systematic literature review on skill development. The study provides insights into the concept of skill development, specifically for academicians to carry out research in a niche area and for government authorities in policy formulation.
Modelling internet of things driven sustainable food security system
Authors: Kaur Harpreet 
Published: June 2019

PurposeThe purpose of this paper is to model the sustainable food security system using various technologies driving internet of things (IoT). The right to food is a fundamental right of humans. With increasing population and urbanization, less land is being used for agricultural purposes. In addition, the climate change due to global warming often leads to frequent disasters such as droughts and floods, adversely affecting the food production. This leads to increased levels of poverty and hunger. Ensuring food security has become the prime agenda for all the policymakers and government bodies across the world. With changing global dynamics, traditional ways of ensuring food security will not be sufficient alone.Design/methodology/approachThere is a need to develop a sustainable food security system that not only focusses on food production but also equally emphasizes on the efficacy of food distribution and reducing food wastage. In this digital age, the emerging disruptive technologies like Block chain, robotics, big data analytics, and cloud computations, etc., are increasingly changing the functioning of various sectors, giving rise to IoT-based working environment. The policymakers are also exploring these technologies to maximize their outreach so as to benefit the larger set of population and to gain visualization and control over policy implementation using IoT. This paper attempts to model the sustainable food security system using various technologies driving IoT. It also studies the interrelationship among various technologies and their application in various levels of policy implementation. The methodology used in the paper is fuzzy-TISM, which not only provides the causal relationship among two technologies but also provides the magnitude of the cause–effect relationship and the hierarchical framework for the complex problem.FindingsThe paper is addressed to the design of sustainable food security system in the Indian context wherein government ensures food security for all, using public distribution system (PDS).Social implicationsThe paper is addressed to the design of sustainable food security system in Indian context wherein government ensures food security for all, using PDS.Originality/valueThis study is a novel attempt to integrate the IoT into the design of the PDS to ensure food security. The enabling factors for IOT are modelled using Fuzzy-TISM.
Practical model of the axial magnetic field in the end region of large turbo-generators
Authors: Demian Cristian , Romary Raphael , Vogt Gilles , Costan Valentin 
Published: June 2019

Purpose
The axial magnetic field occurs in the end-region of large turbo-generators is known to induce hot points or voltages between laminations, that may cause insulation breakdown and thus stator faults.

Design/methodology/approach
It is important to dispose of simple methods for estimating the axial flux rapidly with regard to the operating point of the machine.

Findings
The authors provide a practical model of the axial magnetic field based on a simplified vector diagram. The parameters required to build the vector composition of the flux densities are assessed with a limited number of finite element method simulations of the whole end-region of the machine. These simulations were validated by an experimental test on a real turbo-generator. Then the axial flux density was simply estimated for various operating points.

Originality/value
The originality of the paper concerns the practical model of the axial magnetic field based on a simplified vector diagram.
Construction delay risk taxonomy, associations and regional contexts
Authors: Derakhshanfar Hossein , Ochoa J. Jorge , Kirytopoulos Konstantinos , Mayer Wolfgang , Tam Vivian W.Y. 
Published: June 2019

Purpose
The purpose of this paper is to systematically develop a delay risk terminology and taxonomy. This research also explores two external and internal dimensions of the taxonomy to determine how much the taxonomy as a whole or combinations of its elements are generalisable.

Design/methodology/approach
Using mixed methods research, this systematic literature review incorporated data from 46 articles to establish delay risk terminology and taxonomy. Qualitative data of the top 10 delay risks identified in each article were coded based on the grounded theory and constant comparative analysis using a three-stage coding approach. Word frequency analysis and cross-tabulation were used to develop the terminology and taxonomy. Association rules within the taxonomy were also explored to define risk paths and to unmask associations among the risks.

Findings
In total, 26 delay risks were identified and grouped into ten categories to form the risk breakdown structure. The universal delay risks and other delay risks that are more or less depending on the project location were determined. Also, it is realized that delays connected to equipment, sub-contractors and design drawings are highly connected to project planning, finance and owner slow decision making, respectively.

Originality/value
The established terminology and taxonomy may be used in manual or automated risk management systems as a baseline for delay risk identification, management and communication. In addition, the association rules assist the risk management process by enabling mitigation of a combination of risks together.
How brand visual aesthetics foster a transnational imagined community
Authors: Buschgens Mark , Figueiredo Bernardo , Rahman Kaleel 
Published: June 2019

Purpose
The purpose of this paper is to investigate how visual aesthetic referents used in branding can help foster a transnational imagined community (TIC). The authors use brands embedded with Middle Eastern visual aesthetics as a research context. As such, the study aims to examine how Middle Eastern non-figurative art is used by non-Middle Eastern brands to foster an imagined Middle Easternness.

Design/methodology/approach
Through a critical visual analysis, the authors apply a visual social semiotic approach to Middle Eastern art canons to better understand the dimensions of transnational imagined communities.

Findings
The study finds and discusses six sub-dimensions of Middle Easternness, which compose two overarching dimensions of TIC, namely, temporal and spatial. These sub-dimensions provide brand managers and designers with six different ways to foster transnational imagined communities through the use of visual aesthetic referents in branding.

Research limitations/implications
This research identifies the specific visual sub-dimensions of brands that enable transnational communities to be imagined.

Practical implications
Understanding the visual aesthetic sub-dimensions in this study provides brand managers with practical tools that can help develop referents that foster transnational imagined communities in brand building to achieve competitive advantage and reach a transnational segment.

Originality/value
Prior studies have primarily focussed on how visual aesthetics help in understanding issues related to national identity. In contrast, this paper examines the use of visual aesthetics in branding from a transnational perspective.
A comparison of topology optimization and genetic algorithms for the optimization of thermal energy storage composites
Authors: Badenhorst Heinrich 
Published: June 2019

Purpose
The purpose of this paper is to apply two optimization methods to the issue of sensible energy store design.

Design/methodology/approach
This paper is a comparison of topology optimization and genetic algorithms.

Findings
Genetic algorithms are prone to converge to local maxima while requiring significantly longer convergence times compared to topology optimization. Topology optimization resulted in structures representing parallel sheets, which are as thin as the grid allows. These configurations can maintain the maximum surface area between the low and high conductivity materials at high refinement, resulting in the best performance.

Practical implications
Time required for 99 per cent store discharge is decreased by 70 per cent using a 50 × 50 optimization grid at a loading of 10 Vol.%.

Originality/value
These approaches have not been compared nor applied to this specific problem before. Value is in the key finding that maximization of surface area is only possible with fins/sheets and not tree structures. This dictates the optimal solution for dynamic behaviour.
Improvement of drug delivery micro-circulatory system with a novel pattern of CuO-Cu/blood hybrid nanofluid flow towards a porous stretching sheet
Authors: Dinarvand Saeed , Nademi Rostami Mohammadreza , Dinarvand Rassoul , Pop Ioan 
Published: June 2019

Purpose
This paper aims to simulate the steady laminar mixed convection incompressible viscous and electrically conducting hybrid nanofluid (CuO-Cu/blood) flow near the plane stagnation-point over a horizontal porous stretching sheet along with an external magnetic field and induced magnetic field effects that can be applicable in the biomedical fields like the flow dynamics of the micro-circulatory system and especially in drug delivery.

Design/methodology/approach
The basic partial differential equations (PDEs) are altered to a set of dimensionless ordinary differential equations (ODEs) with the help of suitable similarity variables which are then solved numerically using bvp4c scheme from MATLAB. Inasmuch as validation results have shown a good agreement with previous reports, the present novel mass-based algorithm can be used in this problem with great confidence. Governing parameters are both nanoparticle masses, base fluid mass, empirical shape factor of both nanoparticles, suction/injection parameter, magnetic parameter, reciprocal magnetic Prandtl number, Prandtl number, heat source parameter, mixed convection parameter, permeability parameter and frequency ratio. The effect of these parameters on the flow and heat transfer characteristics of the problem is discussed in detail.

Findings
It is shown that the use of CuO and Cu hybrid nanoparticles can reduce the hemodynamics effect of the capillary relative to pure blood case. Moreover, as the imposed magnetic field enhances, the velocity of the blood decreases. Besides, when the blade shapes for both nanoparticles are taken into account, the local heat transfer rate is maximum that is also compatible with experimental observations.

Originality/value
An innovative mass-based model of CuO-Cu/blood hybrid nanofluid has been applied. The novel attitude to one-phase hybrid nanofluid model corresponds to considering nanoparticles mass as well as base fluid mass to computing the solid equivalent volume fraction, the solid equivalent density and also solid equivalent specific heat.
Flow and heat transfer of couple stress nanofluid sandwiched between viscous fluids
Authors: Umavathi C. Jawali , Sheremet Mikhail 
Published: June 2019

Purpose
The purpose of this study is a numerical analysis of steady-state heat transfer behavior of couple-stress nanofluid sandwiched between viscous fluids. It should be noted that this research deals with the development of a cooling system for the electronic devices.

Design/methodology/approach
Stokes model is used to define the couple-stress fluid and the single-phase nanofluid model is used to define the nanofluid transport processes. The fluids in all regions are assumed to be incompressible, immiscible and the transport properties in all the three layers are assumed to be constant. The governing coupled linear ordinary differential equations are made dimensionless by using appropriate fundamental quantities. The exact solutions obtained for the velocity and temperature fields are evaluated numerically for various model parameters.

Findings
The results are demonstrated using different types of nanoparticles such as copper, silver, silicon oxide (SiO2), titanium oxide (TiO2) and diamond. The investigations are carried out using copper–water nanofluid for different values of couple-stress parameter a with a range of 0 = a = 12, solid volume fraction ϕ with a range of 0.0 ≤ ϕ ≤ 0.05, Eckert number Ec with a range of 0.001 ≤ Ec ≤ 6 and Prandtl number Pr with a range of 0.001 ≤ Pr ≤ 6. It was found that the Nusselt number increases by increasing the couple stress parameter, Eckert number and Prandtl number and it decreases with a growth of the solid volume fraction parameter. It was also observed that using SiO2–water nanofluid, the optimal Nusselt number is obtained. Further, using copper, silver, diamond and TiO2, nanoparticles and water as a base fluid does not show any significant changes in the rate of heat transfer. The couple-stress parameter enhances the velocity and temperature fields whereas the solid volume fraction suppresses the flow field for both Newtonian and couple-stress fluid.

Originality/value
The originality of this work is to analyze the heat transfer behavior of couple-stress nanofluid sandwiched between viscous fluids. The results would benefit scientists and engineers to become familiar with the analysis of convective heat transfer and flow structures in nanofluids and the way to predict the heat transfer rate in advanced technical systems, in industrial sectors including transportation, power generation, chemical sectors, electronics, etc.