bluuebunny/crossref_metadata_2025_split huggingface dataset:
Maternal-fetal Transmission and Adverse Perinatal Outcomes in Pregnant Women Infected With Zika Virus: A Prospective Cohort Study in French Guiana
Authors: Pomar L. , Vouga M. , Lambert V. , Pomar C. , Hcini N. , Jolivet A. , Benoist G. , Rousset D. , Matheus S. 
Published: May 2019

(BMJ. 2018;363:k4431)
While Zika virus has been confirmed to be vertically transmitted from mothers to infants, and associated with congenital anomalies, the risk of congenital Zika virus syndrome (CZS) is unclear due to a lack of fetal and neonatal testing. The risk of CZS with its associated fetal abnormalities had been reported to be as high as 40% based on the outbreak in Brazil, but more recent reports from the United States suggest a 4% to 8% risk of birth defects in babies born to mothers with confirmed infection. This study aimed to estimate the risk of maternal-fetal transmission of infection as well as the percentage of neonates with clear signs of infection or complications related to CZS within 1 week after birth.
Long-term exposure to fine particulate matter and natural-cause and cause-specific mortality in Japan
Authors: Yorifuji Takashi , Kashima Saori , Tani Yasunari , Yamakawa Junji , Doi Hiroyuki 
Published: May 2019

Background:
A number of studies have linked long-term exposure to particulate matter with aerodynamic diameter <2.5 µm (PM2.5) with mortality, but most of these studies were conducted in Europe and North America. Studies in Asian countries had been conducted at relatively high exposures. We evaluated the association of long-term exposure to PM2.5 and natural-cause and cause-specific mortality in Japan, where PM2.5 levels are relatively low compared with levels in other Asian countries.

Methods:
A cohort of 75,531 participants underwent basic health checkups in Okayama City in 2006 or 2007. We followed the participants until the end of 2016. Average PM2.5 levels from 2006 to 2010 were obtained and assigned to the participants by geographical location. We used the Cox proportional hazard models to estimate hazard ratios for a 5-μg/m3 increase in PM2.5 levels for natural-cause or cause-specific mortality, adjusting for potential confounders.

Results:
PM2.5 exposure was associated with increased risk of mortality; the hazard ratios were 1.29 (95% confidence interval = 1.18, 1.41) for mortality from natural causes, 1.16 (1.02, 1.32) for cardiorespiratory mortality, and 1.63 (1.13, 2.34) for lung cancer mortality. PM2.5 exposure was more strongly associated with cardiorespiratory mortality from hypertension, pneumonia and influenza, and chronic obstructive pulmonary disease than with ischemic heart disease or cerebrovascular disease. Elderly participants and smokers tended to have higher effect estimates.

Conclusion:
Long-term exposure to PM2.5 can increase the risk of natural-cause, cardiorespiratory, and lung cancer mortality in Japan.
Commercial Sexual Exploitation of Children: An Update for the Forensic Nurse
Authors: Hornor Gail , Quinones Saribel Garcia , Bretl Deborah , Courtney Audrey B. , Herendeen Pamela Ann , Lewin Linda , Loyke Julie A. , Morris Kristen , Schapiro Naomi A. , Williams Sheona 
Published: May 2019

ABSTRACT
Commercial sexual exploitation of children (CSEC) is the sexual abuse of children through buying, selling, or trading their sexual services. This may involve engaging a child under the age of 18 years in prostitution, pornography, stripping, exotic dancing, escort services, or other sexual services. CSEC is a problem of epidemic proportions throughout the world including the United States; however, the actual number of CSEC victims in the United States is unknown. Studies indicate that most child victims are seen by a healthcare provider while being trafficked and that many victims receive care at a pediatric hospital within 1 year of their identification as a victim. CSEC is a significant pediatric healthcare problem. It is vital that forensic nurses possess a thorough understanding of the problem and be poised to better identify, intervene, and prevent CSEC. In this article, we focus on risk factors commonly experienced by victims, recruitment strategies used by traffickers, indicators to identify child victims, and intervention and educational strategies of relevance to forensic nurses.
One hundred years after the 1918 pandemic: new concepts for preparing for influenza pandemics
Authors: Pavia Andrew 
Published: May 2019

Purpose of review
In the 100 years since the influenza pandemic of 1918–1919, the most deadly event in human history, we have made substantial progress yet we remain vulnerable to influenza pandemics This article provides a brief overview of important advances in preparing for an influenza pandemic, viewed largely from the perspective of the healthcare system.

Recent findings
We have gained insights into influenza pathogenicity, the animal reservoir and have improved global surveillance for new strains and tools for assessing the pandemic risk posed by novel strains. Public health has refined plans for severity assessment, distribution of countermeasures and nonpharmaceutical approaches. Modest improvements in vaccine technology include cell culture-based vaccines, adjuvanted vaccine and recombinant technology. Conventional infection control tools will be critical in healthcare settings. New evidence suggests that influenza virus may be present in aerosols; the contribution of airborne transmission and role of N95 respirators remains unknown. Baloxavir and pimodivir are new antivirals that may improve treatment, especially for severely ill patients. Optimal use and the risk of resistance require further study.

Summary
Despite the progress in pandemic preparedness, gaps remain including important scientific questions, adequate resources and most importantly, the ability to rapidly deliver highly effective vaccines.
Training Cesarean Section
Authors: Zetner Diana B. , Petersen Iben , Konge Lars , Thinggaard Ebbe 
Published: May 2019

Summary Statement
This study is a scoping review that reviews the existing literature on educational strategies in training of cesarean section. A systematic search was carried out in relevant databases, identifying 28 studies for inclusion. Thematic analysis revealed the following training strategies: simulation-based training (team training, in situ training, technical training), simulators (low-fidelity simulators, high-fidelity simulators), clinical training, e-learning or videos, classroom-based learning (lectures, small groups), and assessment (assessment programs/interventions, assessment of learners). Simulation-based training provides a unique milieu for training in a safe and controlled environment. Simulation-based team training is widely accepted and used in obstetrics and improves nontechnical skills, which are important in emergency cesarean section. High-fidelity simulators are advanced and realistic, but because of the expense, low-fidelity simulators may provide a reasonable method for training surgical skills. The literature in training and assessment of surgical skills in relation to cesarean section is sparse, and more studies are warranted.
Acute imaging for evidence-based treatment of ischemic stroke
Authors: Thomalla Götz , Gerloff Christian 
Published: May 2019

Purpose of review
Until recently, intravenous thrombolysis and mechanical thrombectomy for acute stroke treatment relied on reported information on the known time of symptom onset and was limited to treatment within a narrow time window. New trials have provided evidence for imaging-based effective reperfusion treatment in an extended time window and unknown-onset stroke. This review summarizes evidence from recent clinical trials and suggests a simple algorithm for the choice of imaging modalities to guide evidence-based reperfusion treatment of acute stroke.

Recent findings
Two trials have provided evidence for benefit of mechanical thombectomy in patients with stroke from large vessel occlusion up to 24 h after symptom onset or with unknown symptom onset who have a small infarct core but large perfusion lesion or severe clinical deficit (DAWN, DEFUSE-3). An MRI-based trial has demonstrated the benefit of intravenous thrombolysis with alteplase in patients with unknown onset stroke who present with MRI findings of DWI-FLAIR mismatch (WAKE-UP). Another trial demonstrated benefit of intravenous alteplase in an extended or unknown time-window in patients with penumbral pattern on MRI or CT perfusion (EXTEND).

Summary
In stroke patients with unknown symptom onset or known symptom onset up to 24 h, advanced imaging with MRI or with CT perfusion can guide effective acute reperfusion treatment with mechanical thrombectomy and intravenous alteplase.
One model to rule them all? Modelling approaches across OneHealth for human, animal and plant epidemics
Authors: Kleczkowski Adam , Hoyle Andy , McMenemy Paul 
Published: May 2019

One hundred years after the 1918 influenza outbreak, are we ready for the next pandemic? This paper addresses the need to identify and develop collaborative, interdisciplinary and cross-sectoral approaches to modelling of infectious diseases including the fields of not only human and veterinary medicine, but also plant epidemiology. Firstly, the paper explains the concepts on which the most common epidemiological modelling approaches are based, namely the division of a host population into susceptible, infected and removed (SIR) classes and the proportionality of the infection rate to the size of the susceptible and infected populations. It then demonstrates how these simple concepts have been developed into a vast and successful modelling framework that has been used in predicting and controlling disease outbreaks for over 100 years. Secondly, it considers the compartmental models based on the SIR paradigm within the broader concept of a ‘disease tetrahedron’ (comprising host, pathogen, environment and man) and uses it to review the similarities and differences among the fields comprising the ‘OneHealth’ approach. Finally, the paper advocates interactions between all fields and explores the future challenges facing modellers.This article is part of the theme issue ‘Modelling infectious disease outbreaks in humans, animals and plants: approaches and important themes’. This issue is linked with the subsequent theme issue ‘Modelling infectious disease outbreaks in humans, animals and plants: epidemic forecasting and control’.
Host density dependence and environmental factors affecting laurel wilt invasion
Authors: Choudhury Robin A. , Er Hong L. , Hughes Marc , Smith Jason A. , Pruett Grechen E. , Konkol Joshua , Ploetz Randy C. , Marois James J. , Garrett Karen A. , van Bruggen Ariena H.C. 
Published: May 2019

AbstractHost size, density and distribution, in addition to climate, can affect the likelihood a pathogen will invade and saturate landscapes. Laurel wilt, caused by the vector-borne forest pathogenRaffaelea lauricola, has devastated populations of native Lauraceae in the Southeastern US, and continues to spread. We surveyed 87 plots in six coastal islands in South Carolina, Georgia and North Florida, and one inland site (Archbold Biological Station) in South Florida for laurel wilt-affected and non-affected individual plants belonging to the genusPersea. The coastal island sites were surveyed once in 2008 or 2009, and the inland site was surveyed eight times from 2011 to 2013. Disease incidence per plot ranged from 0% to 96%, with mean disease incidence 45% across all sites. Disease incidence was positively correlated with trunk diameter and density of hosts with trunk diameter > 5 cm, but negatively with the degree of clustering, which was highest for small trees. A recursive partitioning model indicated that higher disease incidence was associated with moderate temperatures, wider trunk diameter, lower relative humidity, and lower wind speeds. Disease progress over time at Archbold followed a Gompertz curve, plateauing at 3% in two years. The dispersal kernel for disease incidence from a focus followed a negative exponential distribution. The number of plots with diseased trees at Archbold was similar for redbay (P. borbonia) and swampbay (P. palustris), but was lower for silkbay (P. humilis). Understanding how host density, size, and diversity interact with environmental effects will help guide future risk prediction efforts.
Ultrastructural heterogeneity of human layer 4 excitatory synaptic boutons in the adult temporal lobe neocortex
Authors: Yakoubi Rachida , Rollenhagen Astrid , von Lehe Marec , Miller Dorothea , Walkenfort Bernd , Hasenberg Mike , Sätzler Kurt , Lübke Joachim HR 
Published: May 2019

AbstractSynapses are fundamental building blocks that control and modulate the ‘behavior’ of brain networks. How their structural composition, most notably their quantitative morphology underlies their computational properties remains rather unclear, particularly in humans. Here, excitatory synaptic boutons (SBs) in layer 4 (L4) of the temporal lobe neocortex (TLN) were quantitatively investigated.Biopsies from epilepsy surgery were used for fine-scale and tomographic electron microscopy to generate 3D-reconstructions of SBs. Particularly, the size of active zones (AZs) and of the three functionally defined pools of synaptic vesicles (SVs) were quantified.SBs were comparably small (∼2.50 μm2), with a single AZ (∼0.13 µm2) and preferentially established on spines. SBs had a total pool of ∼1800SVs with strikingly large readily releasable (∼ 20), recycling (∼ 80) and resting pools (∼850).Thus, human L4 SBs may act as ‘amplifiers’ of signals from the sensory periphery and integrate, synchronize and modulate intra- and extra-cortical synaptic activity.
β-bursts reveal the trial-to-trial dynamics of movement initiation and cancellation
Authors: Wessel Jan R. 
Published: May 2019

AbstractThe neurophysiological basis of motor processes and their control is of tremendous interest to basic researchers and clinicians alike. Notably, both movement initiation and cancellation are accompanied by prominent field potential changes in the β-frequency band (15-29Hz). In trial-averages, movement initiation is indexed by β-band desynchronization over sensorimotor sites, while movement cancellation is signified by β-power increases over (pre)frontal areas. However, averaging misrepresents the true nature of the β-signal. As recent work has highlighted, raw β-band activity is characterized by short-lasting, burst-like events, rather than by steady modulations. To investigate how such β-bursts relate to movement initiation and cancellation in humans, we investigated scalp-recorded β-band activity in 234 healthy subjects performing the Stop-signal task. Four observations were made: First, both movement initiation and cancellation were indexed by systematic, localized changes in β-bursting. While β-bursting at bilateral sensorimotor sites steadily declined during movement initiation, β-bursting increased at fronto-central sites when Stop-signals instructed movement cancellation. Second, the amount of fronto-central β-bursting clearly distinguished successful from unsuccessful movement cancellation. Third, the emergence of fronto-central β-bursting coincided with the latency of the movement cancellation process, indexed by Stop-signal reaction time. Fourth, individual fronto-central β-bursts during movement cancellation were followed by a low-latency re-instantiation of bilateral sensorimotor β-bursting. These findings suggest that β-bursting is a fundamental signature of the motor system, reflecting a steady inhibition of motor cortex that is suppressed during movement initiation, and can be rapidly re-instantiated by frontal areas when movements have to be rapidly cancelled.Significance StatementMovement-related β-frequency (15-29Hz) changes are among the most prominent features of neural recordings across species, scales, and methods. However, standard averaging-based methods obscure the true dynamics of β-band activity, which is dominated by short-lived, burst-like events. Here, we demonstrate that both movement-initiation and cancellation in humans are characterized by unique trial-to-trial patterns of β-bursting. Movement initiation is characterized by steady reductions of β-bursting over bilateral sensorimotor sites. In contrast, during rapid movement cancellation, β–bursts first emerge over fronto-central sites typically associated with motor control, after which sensorimotor β–bursting re-initiates. These findings suggest a fundamentally novel, non-invasive measure of the neural interaction underlying movement-initiation and –cancellation, opening new avenues for the study of motor control in health and disease.
Allelic polymorphism shapes community function in evolving Pseudomonas aeruginosa populations
Authors: Azimi Sheyda , Roberts Aled E. L. , Peng Shengyun , Weitz Joshua S. , McNally Alan , Brown Samuel P. , Diggle Stephen P. 
Published: May 2019

AbstractPseudomonas aeruginosa is an opportunistic pathogen that chronically infects the lungs of individuals with cystic fibrosis (CF) by forming antibiotic resistant biofilms. Emergence of phenotypically diverse isolates within CF P. aeruginosa populations has previously been reported, however, the impact of heterogeneity on social behaviors and community function is poorly understood. Here we describe how this heterogeneity impacts on behavioral traits by evolving the strain PAO1 in biofilms grown in a synthetic sputum medium for 50 days. We measured social trait production and antibiotic tolerance and used a metagenomic approach to analyze and assess genomic changes over the duration of the evolution experiment. We found that (i) evolutionary trajectories were reproducible in independently evolving populations; (ii) over 60% of genomic diversity occurred within the first 10 days of selection. We then focused on quorum sensing (QS), a well-studied P. aeruginosa trait that is commonly mutated in strains isolated from CF lungs. We found that at the population level (i) evolution in sputum medium selected for decreased production of QS and QS-dependent traits; (ii) there was a significant correlation between lasR mutant frequency, the loss of protease and the 3O-C12-HSL signal, and an increase in resistance to clinically relevant β-lactam antibiotics, despite no previous antibiotic exposure. Overall, our findings provide insights into the effect of allelic polymorphism on community functions in diverse P. aeruginosa populations. Further, we demonstrate that P. aeruginosa population and evolutionary dynamics can impact on traits important for virulence and can lead to increased tolerance to β-lactam antibiotics.
Ferroptosis is programmed by the coordinated regulation of glutathione and iron metabolism by BACH1
Authors: Nishizawa Hironari , Matsumoto Mitsuyo , Shindo Tomohiko , Saigusa Daisuke , Kato Hiroki , Suzuki Katsushi , Sato Masaki , Ishii Yusho , Shimokawa Hiroaki , Igarashi Kazuhiko 
Published: May 2019

AbstractFerroptosis is an iron-dependent programmed cell death resulting from alterations of metabolic processes. However, its regulation and physiological significance remain to be elucidated. By analyzing transcriptional responses of murine embryonic fibroblasts exposed to the ferroptosis-inducer erastin, we found that a set of genes related to oxidative stress protection was induced upon ferroptosis. We further showed that the transcription factor BACH1 promoted ferroptosis by repressing the expression of a subset of erastin-inducible genes involved in the synthesis of glutathione or metabolism of intracellular labile iron, including Gclm, Gclc, Slc7a11, Hmox1, Fth1, Ftl1, and Slc40a1. Compared with wild-type mice, Bach1-/- mice showed resistance to myocardial infarction, the seriousness of which was palliated by the iron-chelator deferasirox, which suppressed ferroptosis. Our findings suggest that ferroptosis is programmed at the transcriptional level to induce genes combating labile-iron-induced oxidative stress and executed upon disruption of the balance between the transcriptional induction of protective genes and accumulation of iron-mediated damage. BACH1 is suggested to control the threshold of ferroptosis and to be a therapeutic target for palliating myocardial infarction.
Rapid and sensitive quantitation of glucose and glucose phosphates derived from storage carbohydrates using gas chromatography mass spectrometry
Authors: Young Lyndsay E.A. , Brizzee Corey O. , Macedo Jessica K. A. , Gentry Matthew S. , Sun Ramon C. 
Published: May 2019

ABSTRACTGlycogen is the primary storage carbohydrate in mammals and it is synthesized in most tissues. Glycogen contains covalently attached phosphate groups on hydroxyls of glucose units. The addition of phosphate modulates branching pattern, granular size, and crystallinity of a glycogen molecule, which all impact its accessibility to glycogen interacting enzymes during catabolism. As glycogen architecture modulates its role in metabolism, it is essential to accurately evaluate and quantify phosphate content in glycogen. Simultaneous quantitation of glucose and its phosphate esters is challenging and requires an assay with high sensitivity and a robust dynamic range. Currently, this method is lacking in the field. Herein, we describe a highly-sensitive method for the detection of both glycogen-derived glucose and glucose-phosphate esters utilizing gas-chromatography coupled mass spectrometry. Using this method, we observed higher glycogen levels in the liver compared to skeletal muscle, but skeletal muscle contained much more phosphate esters. These results confirm previous findings and establish the validity of the method. Importantly, this method can detect femtomole levels of glucose and glucose phosphate esters within an extremely robust dynamic range with excellent accuracy and reproducibility. The method can also be easily adapted for the quantification of glucose from plant starch, amylopectin or other biopolymers as well as covalently attached phosphate within them.