bluuebunny/crossref_metadata_2025_split huggingface dataset:
Two Distinct Approaches for CRISPR-Cas9-Mediated Gene Editing in Cryptococcus neoformans and Related Species
Authors: Wang Ping 
Published: June 2018

For genetic studies of the
Cryptococcus
genus, generation of mutant strains is often hampered by a limited number of selectable genetic markers, the tedious process of vector construction, side effects, and other limitations, such as the high cost of acquiring a particle delivery system. CRISPR-Cas9 technology has been demonstrated in
Cryptococcus
for genome editing. However, it remains labor-intensive and time-consuming since it requires the identification of a suitable type III RNA polymerase promoter for gRNA expression. In addition, there may be potential adverse effects caused by constitutive expressions of Cas9 and gRNA. Here, I report the use of a ribonucleoprotein-mediated CRISPR-Cas9 technique for genome editing of
C. neoformans
and related species. Together with the custom-constructed pCnCas9:U6-gRNA vector that allows low-cost and time-saving DNA-based CRISPR-Cas9, my approach adds to the molecular toolbox for dissecting the molecular mechanism of pathogenesis in this important group of fungal pathogens.
Community-based HPV self-collection versus visual inspection with acetic acid in Uganda: a cost-effectiveness analysis of the ASPIRE trial
Authors: Mezei Alex K , Pedersen Heather N , Sy Stephen , Regan Catherine , Mitchell-Foster Sheona M , Byamugisha Josaphat , Sekikubo Musa , Armstrong Heather , Rawat Angeli , Singer Joel , Ogilvie Gina S , Kim Jane J , Campos Nicole G 
Published: June 2018

BackgroundCervical cancer is the leading cause of cancer death for women in Uganda, despite the potential for prevention through organised screening. Community-based self-collected human papillomavirus (HPV) testing has been proposed to reduce barriers to screening.ObjectiveOur objective was to evaluate the cost-effectiveness of the Advances in Screening and Prevention of Reproductive Cancers (ASPIRE) trial, conducted in Kisenyi, Uganda in April 2014 (n=500). The trial compared screening uptake and compliance with follow-up in two arms: (1) community-based (ie, home or workplace) self-collected HPV testing (facilitated by community health workers) with clinic-based visual inspection with acetic acid (VIA) triage of HPV-positive women (‘HPV-VIA’) and (2) clinic-based VIA (‘VIA’). In both arms, VIA was performed at the local health unit by midwives with VIA-positive women receiving immediate treatment with cryotherapy.DesignWe informed a Monte Carlo simulation model of HPV infection and cervical cancer with screening uptake, compliance and retrospective cost data from the ASPIRE trial; additional cost, test performance and treatment effectiveness data were drawn from observational studies. The model was used to assess the cost-effectiveness of each arm of ASPIRE, as well as an HPV screen-and-treat strategy (‘HPV-ST’) involving community-based self-collected HPV testing followed by treatment for all HPV-positive women at the clinic.Outcome measuresThe primary outcomes were reductions in cervical cancer risk and incremental cost-effectiveness ratios (ICERs), expressed in dollars per year of life saved (YLS).ResultsHPV-ST was the most effective and cost-effective screening strategy, reducing the lifetime absolute risk of cervical cancer from 4.2% (range: 3.8%–4.7%) to 3.5% (range: 3.2%–4%), 2.8% (range: 2.4%–3.1%) and 2.4% (range: 2.1%–2.7%) with ICERs of US$130 (US$110–US$150) per YLS, US$240 (US$210–US$280) per YLS, and US$470 (US$410–US$550) per YLS when performed one, three and five times per lifetime, respectively. Findings were robust across sensitivity analyses, unless HPV costs were more than quadrupled.ConclusionsCommunity-based self-collected HPV testing followed by treatment for HPV-positive women has the potential to be an effective and cost-effective screening strategy.
The Veterans Metrics Initiative study of US veterans’ experiences during their transition from military service
Authors: Vogt Dawne , Perkins Daniel F , Copeland Laurel A , Finley Erin P , Jamieson Christopher S , Booth Bradford , Lederer Suzanne , Gilman Cynthia L 
Published: June 2018

PurposeEfforts to promote the health and well-being of military veterans have been criticised for being inadequately informed of veterans’ most pressing needs as they separate from military service, as well as the programmes that are most likely to meet these needs. The current article summarises limitations of the current literature and introduces The Veterans Metrics Initiative (TVMI) study, a longitudinal assessment of US veterans’ well-being and programme use in the first three years after they separate from military service. Veterans were assessed within 3 months of military separation and will complete five additional assessments at 6-month intervals during the subsequent period.ParticipantsThe TVMI study cohort consists of a national sample of 9566 newly separated US veterans that were recruited in the fall of 2016.Findings to dateThe TVMI sample includes representation from all branches of service, men and women, and officers and enlisted personnel. Although representative of the larger population on many characteristics, differential response rates were observed for some subgroups, necessitating the development of non-response bias weights. Comparisons between unweighed and weighted results suggest that the weighting procedure adequately adjusts for observed differences.Future plansAnalyses are under way to examine veterans’ well-being and programme use in the period following separation after military service, as well as factors associated with poor outcomes. We have also begun to decompose programmes into their core components to facilitate examination of how these components relate to well-being. Once our third data collection is complete, we will examine factors related to different patterns of readjustment over time.
Effectiveness of the Gold Standard Programme (GSP) for smoking cessation on smokers with and without a severe mental disorder: a Danish cohort study
Authors: Rasmussen Mette , Klinge Mads , Krogh Jesper , Nordentoft Merete , Tønnesen Hanne 
Published: June 2018

ObjectivesWe compared the effectiveness of an intensive smoking cessation intervention among smokers with and without a severe mental disorder (SMD) and identified factors associated with successful quitting. The main hypothesis was that smokers with an SMD would be less likely to stay continuously smoke-free for 6 months.DesignA prospective cohort study.SettingIn all, 302 smoking cessation clinics in Denmark from municipal clinics, pharmacies, hospitals, midwives, primary care facilities and other private providers who reported data to the national Danish Smoking Cessation Database from 2006 to 2016 participated in this study.ParticipantsA total of 38 293 patients from the Danish Smoking Cessation Database. Patients with an SMD were identified by linking data to the Danish National Patient Register. Diagnoses of organic mental disorders (F0 chapter) or intellectual disabilities (F7 chapter) were not included. Smokers ≥18 years old who were attending a Gold Standard Programme (GSP) with planned follow-up were included. Smokers not wanting contact after 6 months were excluded.InterventionsA comprehensive manual-based smoking cessation intervention comprising five meetings over a 6-week period (the GSP).Main outcome measuresSelf-reported continuous abstinence at the 6-month follow-up.ResultsIn all, 69% of the participants participated in the follow-up after 6 months. The overall rate of successful quitting was high but significantly lower in SMD smokers (29% vs 38%; OR 0.74; 95% CI 0.68 to 0.80). Variables associated with successful quitting were compliance (defined as attending ≥75% of the planned meetings), older age and male gender as well as not being disadvantaged, heavy smoking or recommendation of intervention by health professionals.ConclusionsOnly 29% of smokers with an SMD successfully quit smoking which was significantly lower than the 38% of smokers without an SMD. Compliance was the most important predictor for successful quitting.
Obstacles to diagnosis and treatment of Lyme disease in the USA: a qualitative study
Authors: Hirsch Annemarie G , Herman Rachel J , Rebman Alison , Moon Katherine A , Aucott John , Heaney Christopher , Schwartz Brian S 
Published: June 2018

ObjectiveFor many individuals with Lyme disease, prompt treatment leads to rapid resolution of infection. However, severe complications can occur if treatment is delayed. Our objective was to identify themes around belated diagnosis or treatment of Lyme disease using the General Model of Total Patient Delay (GMTPD).DesignWe conducted a qualitative interview study using indepth telephone interviews.SettingParticipants were patients from a large, integrated health system in the state of Pennsylvania, USA.ParticipantsThere were 26 participants. Participants had to have a diagnosis of Lyme disease between 2014 and 2017 and a positive IgG western blot. We used a stratified purposeful sampling design to identify patients with and without late Lyme disease manifestations. To ensure variation in care experiences, we oversampled patients diagnosed outside of primary care.Outcome measuresWe asked participants about their experience from first Lyme disease symptoms to treatment. We applied an iterative coding process to identify key themes and then synthesised codes into higher order codes representing the GMTPD stages: appraisal delay (symptom to recognition of illness); illness delay (inferring illness to deciding to seek help); behavioural delay (deciding to seek help to the act of seeking help); scheduling delay (seeking help to attending an appointment); and treatment delay (attending appointment to treatment).ResultsAppraisal delay themes included symptom misattribution, intermittent symptoms and misperceptions about the necessity of a bull’s-eye rash. Health insurance status was a driver of illness and behavioural delays. Scheduling delay was not noted by participants, in part, because 10 of the 26 patients went to urgent care or emergency department settings. Misdiagnoses were more common in these settings, contributing to treatment delay.ConclusionOur study identified potentially modifiable risk factors for belated treatment. Targeting these risk factors may minimise time to treatment and reduce the occurrence of preventable complications.
Observational study of associations between visual imagery and measures of depression, anxiety and post-traumatic stress among active-duty military service members with traumatic brain injury at the Walter Reed National Military Medical Center
Authors: Kaimal Girija , Walker Melissa S , Herres Joanna , French Louis M , DeGraba Thomas J 
Published: June 2018

ObjectivesThe study aimed tocompare recurring themes in the artistic expression of military service members (SMs) with post-traumatic stress disorder (PTSD), traumatic brain injury and psychological health (PH) conditions with measurable psychiatric diagnoses. Affective symptoms and struggles related to verbally expressing information can limit communication in individuals with symptoms of PTSD and deployment-related health conditions. Visual self-expression through art therapy is an alternative way for SMs with PTSD and other PH conditions to communicate their lived experiences. This study offers the first systematic examination of the associations between visual self-expression and standardised clinical self-report measures.DesignObservational study of correlations between clinical symptoms of post-traumatic stress, depression and anxiety and visual themes in mask imagery.SettingThe National Intrepid Center of Excellence at the Walter Reed National Military Medical Center, Bethesda, Maryland, USA.ParticipantsActive-duty military SMs (n=370) with a history of traumatic brain injury, post-traumatic stress symptoms and related PH conditions.InterventionThe masks used for analysis were created by the SMs during art therapy sessions in week 1 of a 4-week integrative treatment programme.Primary outcomesAssociations between scores on the PTSD Checklist–Military, Patient Health Questionnaire-9 and Generalized Anxiety Disorder 7-item scale on visual themes in depictions of aspects of individual identity (psychological injury, military symbols, military identity and visual metaphors).ResultsVisual and clinical data comparisons indicate that SMs who depicted psychological injury had higher scores for post-traumatic stress and depression. The depiction of military unit identity, nature metaphors, sociocultural metaphors, and cultural and historical characters was associated with lower post-traumatic stress, depression and anxiety scores. Colour-related symbolism and fragmented military symbols were associated with higher anxiety, depression and post-traumatic stress scores.ConclusionsEmergent patterns of resilience and risk embedded in the use of images created by the participants could provide valuable information for patients, clinicians and caregivers.
Analysis of cases managed by the otolaryngology service in the Chinese military peacekeeping level 2 medical treatment facility in Mali
Authors: Zhao Yongqiang , Ma X , Li Y , Fu Q 
Published: June 2018

ObjectivesAs part of the UN peacekeeping mission in Mali, the People’s Republic of China have deployed a level 2 medical treatment facility (CHN L2). The aim of this study was to review the cases managed by the otolaryngology service within this facility.MethodsThe medical records of all patients treated by the otolaryngology service at the CHN L2 from 1 March 2015 to 1 March 2018 were retrospectively assessed.Results614/10189 (6%) of all cases seen in the hospital during this period were referred to the otolaryngology service. 7/614 cases required admission to hospital (1.14%) and 40/614 cases required surgery (6.51%). 3/40 cases requiring surgery (7.5%) were performed under general anaesthesia and 37 cases (92.5%) were under local anaesthesia. The most common surgical treatment was facial soft-tissue injury debridement and closure. Acute rhinosinusitis was the most common diagnosis, followed by acute pharyngitis and allergic rhinitis. Four patients required medical evacuation to a level 3 medical treatment facility.ConclusionsDisease non-battle injury in the form of ear disease was the most common presentation. Maxillofacial soft-tissue injury was the most common cause of traumatic injury. There were limits that the service could provide in terms of medical equipment and consumables, necessitating increased training of otolaryngologists prior to deployment.
Use of zonal distribution of lung crackles during inspiration and expiration to assess disease severity in idiopathic pulmonary fibrosis
Authors: Key Angela L , Holt Kimberley , Warburton Christopher J , Walker Paul Phillip , Earis John E 
Published: June 2018

AbstractPurpose of the studyTo measure the number and distribution of crackles in patients with idiopathic pulmonary fibrosis (IPF) and assess how this relates to measures of disease severity.Study designFourteen patients with IPF had both the number of crackles per litre of lung volume and lung function measured every 3 months for 1 year. Crackle counts were expressed according to position (upper and lower zones) and whether they occurred during inspiration and expiration.ResultsAt baseline, crackle count per unit volume was higher at the bases than the apices and higher during inspiration than during expiration. There was a significant relationship between lung function and number of crackles per unit volume. Upper zone crackles during inspiration (crackle count vs forced vital capacity (FVC): r=0.69, p=0.007) and lower zone crackles during expiration (crackle count vs FVC: r=0.55, p=0.04) demonstrated the strongest relationship with lung function.ConclusionsNumber and distribution of crackles in IPF relate to physiological measures of disease severity. Inspiratory lower zone crackles were universal and extensive but the presence, hence, development of inspiratory upper zone crackles and expiratory lower zone crackles correlated with measures of poorer lung function. The presence or appearance of these assessed using chest auscultation provides a clinician with simple measure of disease severity, and possibly progression, prompting further physiological assessment and review of treatment.
Determinants of progression of nicotine dependence symptoms in adolescent waterpipe smokers
Authors: Bahelah Raed , Ward Kenneth D , Ben Taleb Ziyad , DiFranza Joseph R , Eissenberg Thomas , Jaber Rana , Maziak Wasim 
Published: June 2018

IntroductionWaterpipe smoking continues to pose significant challenges to tobacco control efforts and many adolescent waterpipe smokers experience symptoms of nicotine dependence (ND). This study examined the predictors of progression of ND symptoms in adolescent waterpipe smokers.MethodsWe assembled a cohort of Lebanese adolescents enrolled in eighth and ninth grades at baseline, and surveyed them every 6 months over the next 24 months. Progression of ND symptoms was defined as an increase over time in the number of items endorsed on the Hooked on Nicotine Checklist. Predictors included individual (eg, psychological factors, attitudes towards waterpipe, school performance, physical activity) and socioenvironmental (eg, smoking among parents, siblings and close friends; the café environment) attributes.ResultsAmong 264 adolescent waterpipe smokers (baseline mean age=14.6 years, 50.4% females), 105 (39.7%) progressed on ND symptoms during follow-up. Predictors of progression were higher baseline stress (OR 1.11, 95% CI 1.03 to 1.19) and higher self-esteem (OR 1.14, 95% CI 1.05 to 1.24). Reporting difficulty refraining from smoking waterpipe while in a restaurant was the strongest predictor of progression (OR 4.04, 95% CI 1.44 to 11.34). Having a mother with ≥12 years of education protected against progression of ND (OR 0.46, 95% CI 0.21 to 0.99).ConclusionsA significant proportion of adolescent waterpipe smokers progressed on ND. Venue-based policies such as limiting youth access to waterpipe cafés are warranted.
Evaluation of flazasulfuron for hair fescue (Festuca filiformis) suppression and wild blueberry (Vaccinium angustifoliumAit.) tolerance
Authors: Zhang Linshan , White Scott N. , Randall Olson A. , Pruski Kris 
Published: June 2018

Flazasulfuron was evaluated for crop safety and efficacy on hair fescue (Festuca filiformis Pourr.) in wild blueberry (Vaccinium angustifolium Ait.) fields. Treatments consisted of flazasulfuron applied at 38 and 50 g a.i. ha−1in fall of the bearing year and spring and fall of the non-bearing year. Industry standard fall bearing year pronamide (2240 g a.i. ha−1) and spring non-bearing year terbacil (2000 g a.i. ha−1) and foramsulfuron (35 g a.i. ha−1) applications were included for comparison. Pronamide consistently reduced flowering hair fescue tuft density in the non-bearing and bearing years across sites. Terbacil reduced flowering tuft inflorescence height in the non-bearing year, but did not reduce total or flowering tuft density in either year. Foramsulfuron reduced flowering tuft density and flowering tuft inflorescence number and height in the non-bearing year, but hair fescue recovered in the bearing year. Fall bearing year flazasulfuron applications did not reduce total or flowering tuft density or flowering tuft inflorescence number and height. In contrast, spring non-bearing year flazasulfuron applications reduced flowering tuft density and flowering tuft inflorescence number and height, though hair fescue recovered in these treatments in the bearing year. Fall non-bearing year flazasulfuron applications reduced hair fescue total tuft density, flowering tuft density, and flowering tuft inflorescence number in the bearing year. No treatments caused significant injury to wild blueberry. Flazasulfuron may contribute to improved hair fescue management in wild blueberry, and this herbicide should be evaluated further to confirm crop tolerance and identify potential use patterns with currently registered herbicides.
Influence of irrigation and plant canopy architecture on white mould disease of dry bean
Authors: Kader Kazi A. , Balasubramanian Parthiba M. , Chatterton Syama 
Published: June 2018

White mould caused by the fungus Sclerotinia sclerotiorum (Lib.) de Bary is a major constraint to irrigated dry bean production in southern Alberta. Irrigation, coupled with dry bean canopy architecture, may influence white mould by creating conducive environmental conditions. Field experiments were conducted from 2015 to 2017 at Lethbridge to determine the effect of three irrigation levels and five dry bean genotypes with different canopy architectures on white mould. Sensors and data loggers were established to monitor micro-climate data including soil moisture within the top 5 cm, leaf wetness, and soil temperature under the canopy. Canopy porosity, lodging, flower infection, and white mould disease severity were also measured. Higher moisture within the top 5 cm of the soil, lower soil temperature, elevated leaf wetness, and higher white mould incidence were observed in high irrigation plots compared with medium and low irrigation plots. Cultivars varied for leaf wetness, porosity, and lodging. Although a significant interaction between irrigation and cultivar was detected, irrigation levels did not affect disease severity significantly. Lower disease severity and incidence were recorded in AAC Burdett and Island. These cultivars have an upright growth habit, high canopy porosity, and lodging resistance, and therefore, exhibited partial field resistance (avoidance) to white mould. Mean yield across all cultivars was not affected by irrigation; however, the highest yield occurred in the medium irrigation plots. A reduced level of irrigation and development of cultivars with both avoidance and partial physiological resistance may reduce white mould severity and incidence in dry bean fields in Alberta.
New insights into the building of the Variscan Belt in Eastern Europe (Romania, Serbia, Bulgaria)
Authors: Plissart Gaëlle , Diot Hervé , Monnier Christophe , Mărunţiu Marcel 
Published: June 2018

Abstract

The basement of the Alpine Upper Danubian/Balkan nappe, dismembered between Romania, Serbia and Bulgaria, contains evidence of the Variscan orogenic evolution (Lower Devonian Balkan–Carpathian ophiolite, Carboniferous granites). Our study presents a new tectono-metamorphic interpretation of this basement and documents two main deformation phases, D
1
and D
2
. D
1
is a right-lateral thrust recorded in the metagabbroic rocks at the base of the ophiolitic nappe that also affects the underlying units. This phase is related to the ophiolite emplacement on a northerly margin with a top-to-the-palaeo-WNW (Variscan coordinates) at
c.
360 Ma. D
2
records a collisional event in a sinistral transpressive regime. In a zone of sheared folds, it juxtaposed low- (Eşelniţa metasediments) to high-grade metamorphic rocks (Corbu rocks: 600°C/ 5.2 kbar). Syntectonic granitic intrusions later heated these rocks locally before their final cooling, still during the D
2
phase, and with localized circulation of fluids. The sinistral transpressive regime would prevail after the docking of the Balkans and the Sredna Gora terranes separating the Balkan–Carpathian oceanic basin and could correspond to left-lateral escape due to large-scale readjustments between both terranes during the Carboniferous. The Upper Danubian/Balkan basement appears to be located more northerly in the Variscan Belt than the other intra-Alpine basements (Getic, Western Carpathians, Eastern Alps, Western Alps).