bluuebunny/crossref_metadata_2025_split huggingface dataset:
Application of multiple‐response optimization methods for the ergonomic evaluation of carpet weaving knife
Authors: Singh Ashish Kumar , Meena Makkhan Lal , Chaudhary Himanshu 
Published: January 2019

AbstractThe aim of this study is to evaluate several weaving knife configurations in response to multiple criteria’s for optimum design. Nine different knife combinations were tested over ten female weavers in a field experiment. Three levels of blade width and angular orientations were taken as the independent variables. Output response about goniometry and electromyography were taken as dependent variables. Multivariate analysis was carried out to estimate the effect of independent variables on output responses. To tackle the difficulty of knife selection with several output responses, three multicriteria optimization approaches have been compared. Multivariate analysis of variance results showed that the effect of both blade width and angle were significant at wrist angles. Also, the magnitude of effect size on both blade width and the angle was highest for the wrist angles. Despite the same weighing criteria, a slight difference was evident between the ranks from optimization methods. The higher blade widths and smaller angular orientations should be recommended for reduction of wrist deviation and muscle activity during weaving. The best possible combination of knife evaluated using multicriteria optimization was 25 mm × 100°.
A Visible‐Light‐Driven Molecular Motor Based on Pyrene
Authors: Roke Diederik , Feringa Ben L. , Wezenberg Sander J. 
Published: January 2019

AbstractThe aromatic core of an overcrowded alkene‐based molecular motor is extended with the goal of inducing isomerization with visible light instead of harmful UV light. In our design, the common naphthalene moiety in the upper half of the motor is changed to pyrene. The photochemical and thermal isomerization processes are studied in detail using DFT calculations as well as NMR and UV/VIS spectroscopy. Our studies confirm that extension of the π‐system of the upper half successfully leads to a shift of the excitation wavelength into the visible region, while retaining proper rotary function.
Molecular evidence of role of vitamin D deficiency in various extraskeletal diseases
Authors: Zendehdel Abolfazl , Arefi Mohammad 
Published: January 2019

AbstractBackgroundRole of vitamin D is not only limited to skeletal system but various other systems of the body, such as immune system, endocrine system, and cardiopulmonary system.Materials and MethodsIt is supported by the confirmations of systems‐wide expression of vitamin D receptor (VDR), endocrinal effect of calcitriol, and its role in immune responses.ResultsExpression of VDR in various systems, immunoregulatory and hormonal response of vitamin D and deficiency of vitamin D may establish various pathologies in the body.ConclusionThis review provides molecular evidence of relation of vitamin D with extra skeletal.
Long noncoding RNA cancer susceptibility candidate 2 suppresses papillary thyroid carcinoma growth by inactivating the AKT/ERK1/2 signaling pathway
Authors: Huang Fei , Zhang Qing , Chen Wei , Zhang Huiting , Lu Guofen , Chen Jingyu , Qiu Changhong 
Published: January 2019

AbstractObjectivesCancer susceptibility candidate 2 (CASC2) and long noncoding RNA (lncRNA) have been identified as a tumor suppressor in colorectal, lung, renal, and stomach cancer as well as in patient gliomas, but the function of CASC2 in papillary thyroid carcinoma (PTC) is not yet clear. The present study aimed to explore the effects of CASC2 in PTC.MethodsThe CASC2 expression was measured in PTC samples and normal tissues by using quantitative real‐time polymerase chain reaction. The lentiviral vectors were used to establish CASC2 overexpression models in PTC cell lines to determine the effects of CASC2 on cell proliferation, apoptosis, migration, and invasion. A tumor xenograft animal model was used to examine the functions of overexpression CASC2.ResultsCASC2 expression was significantly decreased in PTC tumor tissues than adjacent normal tissues. CASC2 downregulation in PTC tissues significantly correlated with the tumor size, the presence of multifocal lesions, and the advanced pathological stage. CASC2 overexpression suppressed the cell proliferation and promoted apoptosis in PTC cell lines and CASC2 overexpression resulted in the inactivation of protein kinase B (PKB/AKT) and extracellular signal‐regulated kinases (ERK1/2). The regulatory effects of CASC2 on PTC cell biological behavior were further enhanced by mitogen‐activated protein kinase kinase (MEK) inhibitor U0126 or AKT1/2/3 inhibitor MK‐2206 2HCl. CASC2 overexpression suppressed tumor growth in PTC cells in xenograft mouse models.ConclusionOur results indicated that CASC2 significantly suppressed tumorigenesis in PTC and CASC2 may serve as a novel prognostic marker or therapeutic target.
Homeodomain interacting protein kinase‐2 phosphorylates FOXM1 and promotes FOXM1‐mediated tumor growth in renal cell carcinoma
Authors: Liu Feng , Li Na , Liu Yingying , Zhang Jing , Zhang Jin , Wang Zhixin 
Published: January 2019

AbstractForkhead Box M1 (FOXM1) is a member of the forkhead/winged‐helix transcription factors regulating proliferation‐associated genes and is critical to DNA replication and mitosis. With this said, the function of FOXM1 in renal cell carcinoma (RCC) has not been clearly elucidated. Thus, in this study, the expression pattern of FOXM1 was significantly upregulated in RCC tissues compared with adjacent noncancerous tissues. Moreover, using liquid chromatography with tandem mass spectrometry (LC‐MS/MS), FOXM1 can interact with homeodomain interacting protein kinase‐2 (HIPK2). In addition, FOXM1 can be phosphorylated by HIPK2. Furthermore, HIPK2 knockdown inhibits FOXM1 phosphorylation and reduces transcription of FOXM1 associated genes: Cyclin B1 and Aurora B. In addition, HIPK2 knockdown hampers the RCC cells cycle progression and suppresses cell viability in vivo and in vitro. In conclusion, the phosphorylation of FOXM1 by HIPK2 can promote FOXM1 transcription activity and cell proliferation in RCC, thus, indicating a potential mechanism for the treatment of human RCC in the future.
VEGF gene rs3025039C/T and rs833052C/A variants are associated with bladder cancer risk in Asian descendants
Authors: Zhang Li‐Feng , Ren Ke‐Wei , Zuo Li , Zou Jian‐Gang , Song Ning‐Hong , Mi Yuan‐Yuan , Wang Zeng‐Jun , Zhang Wei 
Published: January 2019

AbstractIntroductionPolymorphisms of vascular endothelial growth factor (VEGF) gene were evaluated in a number of studies to evaluate bladder cancer (BCa) susceptibility but with controversial conclusions.Material and MethodsWe performed a pooled analysis and used odds ratios (ORs) with corresponding 95% confidence intervals (95% CIs) to investigate the correlation between VEGF gene rs3025039C/T and rs833052C/A variants and risk of BCa. Furthermore, we utilized in silico tools to demonstrate the relationship of
VEGF expression correlated with BCa susceptibility and survival time.ResultsA total of eight studies including 4359 BCa patients and 5417 control subjects were enrolled in our study. For VEGF rs3025039C/T, a significant association was indicated between this variant and BCa risk in homozygote comparison (OR = 1.51; 95% CI = 1.13‐2.02;
P
heterogeneity = 0.815) and recessive genetic model (OR = 1.49; 95% CI = 1.12‐1.99;
P
heterogeneity = 0.874), in particular in an Asian population subgroup. For
VEGF rs833052C/A, we observed a positive association between this variant and BCa susceptibility in Asian descendants. Results from in silico tool showed evidence that
VEGF expression in bladder carcinoma tissue is higher than that in normal counterpart (transcripts per kilobase million = 7.21 vs 6.85;
P < 0.05).ConclusionsThe VEGF gene rs3025039C/T and rs833052C/A variants may contribute to the risk of developing BCa, especially in Asian descendants. Future larger sample studies should be continued to focus on this issue in more detail.
Struggling to be a person: Lived experience of avoidant personality disorder
Authors: Sørensen Kristine D. , Råbu Marit , Wilberg Theresa , Berthelsen Eivind 
Published: January 2019

AbstractObjectiveTo better understand the subjective lived experience of persons diagnosed with avoidant personality disorder (AVPD).MethodsPersons with an AVPD (N = 15) were interviewed twice with semistructured qualitative interviews and analyzed through interpretative–phenomenological analysis. Persons with first‐hand experience of AVPD were included in the research process.ResultsThe superordinate theme, “struggling to be a person,” encompassed two main themes. The first, “fear and longing,” incorporated the subthemes “longing for connection,” “dreading to get close” and “being alone, for better or for worse.” The second main theme, “a doubting self,” included the subthemes “feeling insecure” and “searching for a sense of self.”ConclusionsThe findings shed light on how the reflexive selves of people with AVPD might struggle with sense‐making, sense of agency, and identity. This study underscores how impaired tacit knowledge of social behavior can hamper the process of being a person in relation to others.
Melatonin alleviates the deterioration of oocytes from mice subjected to repeated superovulation
Authors: Xiao Peng , Nie Junyu , Wang Xuefang , Lu Kehuan , Lu Shengsheng , Liang Xingwei 
Published: January 2019

AbstractInduction of repeated superovulation with exogenous hormones is widely used in assisted reproductive technology (ART). Though it is generally safe, emerging evidence has indicated that repeated superovulation may compromise oocyte quality. However, few studies have explored how to ameliorate such impairment. Because melatonin has beneficial influences on oocytes in various detrimental environments, we aimed to explore whether melatonin could protect mouse oocytes after repeated superovulation. We found that repeated superovulation markedly reduced meiotic maturation and disrupted spindle organization and chromosome alignment. Furthermore, we observed reduced mitochondrial content and enhanced early apoptosis in oocytes from mice subjected to repeated superovulation. In addition, 5‐methylcytosine (5mc) fluorescence intensity was lower in oocytes from experimental mice than in those from control mice, indicating that repeated superovulation disrupts genomic DNA methylation, and elevations in reactive oxygen species levels indicated that repeated superovulation also induces oxidative stress. Conversely, melatonin administration improved oocyte maturation and attenuated the observed defects. Interestingly, supplementation with melatonin during in vitro maturation had the same protective effects on oocytes as in vivo melatonin administration. In summary, our results show that melatonin can improve oocyte quality after repeated superovulation and thus provide a potential strategy to improve ART efficiency.
Microarray expression profiles analysis revealed lncRNA OXCT1‐AS1 promoted bladder cancer cell aggressiveness via miR‐455‐5p/JAK1 signaling
Authors: Chen Jin‐Bo , Zhu Ye‐Wen , Guo Xi , Yu Cui , Liu Pei‐Hua , Li Chao , Hu Jiao , Li Hui‐Huang , Liu Long‐Fei , Chen Min‐Feng , Chen He‐Qun , Xiong‐Bing Zu 
Published: January 2019

AbstractBladder cancer (BCa) is one of the most prevalent cancers of the urinary system worldwide. Accumulating evidence suggests that long noncoding RNAs (lncRNAs) perform a vital function in the pathogenesis and progression of BCa. In the current study, we identified a novel lncRNA OXCT1‐AS1 and investigated its role and potential mechanisms in BCa. The microarray results showed the expression of lncRNAs, microRNAs, and messenger RNAs between BCa primary tumor tissues and metastatic lymph nodes were significantly different. The quantitative polymerase chain reaction verification was performed to ensure the reliability of the screening results. The Cell Counting Kit 8 and transwell assay were used to assess the tumor cell proliferation and invasion abilities in vitro, respectively. The dual‐luciferase activity assay was performed to investigate the potential mechanism of competing endogenous RNA network. lncRNA OXCT1‐AS1, which elevated in metastasis lymph node, was significantly upregulated in BCa cell lines compared with SVHUC‐1. We demonstrated OXCT1‐AS1 inhibited miR‐455‐5p to decrease its binding to the JAK1 3′‐untranslated region, which could upregulate the expression of JAK1 at the protein level, thus promoting BCa proliferation and invasion. Therefore, lncRNA OXCT1‐AS1 could act as a potential biomarker and therapeutic target for patients with BCa.
The relationship between microRNAs and Rab family GTPases in human cancers
Authors: Shahabi Arman , Naghili Behrooz , Ansarin Khalil , Zarghami Nosratollah 
Published: January 2019

AbstractmicroRNAs (miRNAs), as a group of noncoding RNAs, posttranscriptionally control gene expression by binding to 3′‐untranslated region (3′‐UTR). Ras‐associated binding (Rab) proteins function as molecular switches for regulating vesicular transport, which mainly have oncogenic roles in cancer development and preventing the efficacy of chemotherapies. Increased evidence supported that miRNAs/Rabs interaction have been determined as potential therapeutics for cancer therapy. Nevertheless, instability and cross‐targeting of miRNAs are main limitations of using miRNA‐based therapeutic. The mutual interplay between Rabs and miRNAs has been poorly understood. In the present review, we focused on the essence and activity of these molecules in cancer pathogenesis. Also, numerous hindrances and potential methods in the expansion of miRNA as an anticancer therapeutics are mentioned.
Establishment and optimization of epithelial cell cultures from human ectocervix, transformation zone, and endocervix optimization of epithelial cell cultures
Authors: Deng Han , Mondal Sumona , Sur Shantanu , Woodworth Craig D. 
Published: January 2019

AbstractCervical cancer is a major public health problem and research using cell culture models has improved understanding of this disease. The human cervix contains three anatomic regions; ectocervix with stratified squamous epithelium, endocervix with secretory epithelium, and transformation zone (TZ) with metaplastic cells. Most cervical cancers originate within the TZ. However, little is known about the biology of TZ cells or why they are highly susceptible to carcinogenesis. The goal of this study was to develop and optimize methods to compare growth and differentiation of cells cultured from ectocervix, TZ or endocervix. We examined the effects of different serum‐free media on cell attachment, cell growth and differentiation, and cell population doublings in monolayer culture. We also optimized conditions for organotypic culture of cervical epithelial cells using collagen rafts with human cervical stromal cells. Finally, we present a step‐by‐step protocol for culturing cells from each region of human cervix.
Curcumin as a therapeutic agent in leukemia
Authors: Kouhpeikar Hamideh , Butler Alexandra E. , Bamian Faeze , Barreto George E. , Majeed Muhammed , Sahebkar Amirhossein 
Published: January 2019

AbstractLeukemia comprises a group of hematological malignancies responsible for 8% of all cancers and is the most common cancer in children. Despite significant improvements in leukemia treatment, the efficacy of conventional chemotherapeutic agents is low and the disease carries a poor prognosis with frequent relapses and high mortality. Curcumin is a yellow polyphenol compound with diverse pharmacological actions including anticancer, antioxidant, antidiabetic, anti‐inflammatory, immunomodulatory, hepatoprotective, lipid‐regulating, antidepressant, and antiarthritic. Many cellular and experimental studies have reported the benefits of curcumin in treating leukemia. Curcumin's anticancer effects are exerted via various mechanisms. Here, we review the effects of curcumin on various types of leukemia whilst considering its mechanisms of action.
Effects of the isothiocyanate sulforaphane on TGF‐β1‐induced rat cardiac fibroblast activation and extracellular matrix interactions
Authors: Fix Charity , Carver‐Molina Amanda , Chakrabarti Mrinmay , Azhar Mohamad , Carver Wayne 
Published: January 2019

AbstractAn important step in many pathological conditions, particularly tissue and organ fibrosis, is the conversion of relatively quiescent cells into active myofibroblasts. These are highly specialized cells that participate in normal wound healing but also contribute to pathogenesis. These cells possess characteristics of smooth muscle cells and fibroblasts, have enhanced synthetic activity secreting abundant extracellular matrix components, cytokines, and growth factors, and are capable of generating contractile force. As such, these cells have become potential therapeutic targets in a number of disease settings. Transforming growth factor β (TGF‐β) is a potent stimulus of fibrosis and myofibroblast formation and likewise is an important therapeutic target in several disease conditions. The plant‐derived isothiocyanate sulforaphane has been shown to have protective effects in several pathological models including diabetic cardiomyopathy, carcinogenesis, and fibrosis. These studies suggest that sulforaphane may be an attractive preventive agent against disease progression, particularly in conditions involving alterations of the extracellular matrix and activation of myofibroblasts. However, few studies have evaluated the effects of sulforaphane on cardiac fibroblast activation and their interactions with the extracellular matrix. The present studies were carried out to determine the potential effects of sulforaphane on the conversion of quiescent cardiac fibroblasts to an activated myofibroblast phenotype and associated alterations in signaling, expression of extracellular matrix receptors, and cellular physiology following stimulation with TGF‐β1. These studies demonstrate that sulforaphane attenuates TGF‐β1‐induced myofibroblast formation and contractile activity. Sulforaphane also reduces expression of collagen‐binding integrins and inhibits canonical and noncanonical TGF‐β signaling pathways.
Scrutinizing Ecce Homo: European or Indian painting? Assessment by Raman and complementary spectroscopic techniques
Authors: Antunes Vanessa , Serrão Vitor , Candeias António , Mirão José , Cardoso Ana , Carvalho Maria L. , Fernandes Natasha , Manso Marta 
Published: January 2019

AbstractThis work intends to characterize producing techniques and materials used in Ecce Homo painting (P1) from the beginning of the 17th century, made at the time of Iberian Union government in Goa, which belongs to Museum of Christian Art at Old Goa (India). Determination of the manufacturing processes, material characterization of ground/priming layer, and pigment layers were the scopes of this study.Knowledge of Goan painting materials at this epoch is an almost unknown issue. For this study, cross sections were analyzed by micro‐Raman spectroscopy to identify core materials, complemented to other techniques: optical microscopy, Energy Dispersive X‐ray Fluorescence, scanning electron microscopy‐energy dispersive spectroscopy, and micro‐Fourier Transform Infrared spectroscopy. Paintings were also examined by infrared reflectography.By scrutinizing this painting, scientific confirmation on materials and technique was achieved, and the comparison with two other paintings, one Goan (P2) and another Portuguese (P3), from the same period and under the same theme was established.Results allowed identification and origin of paintings under study, considered as a Goan feature by art history, bringing also new data for painting conservation decision making.
Evaluation of Robustness of the Biosurfactant Derived from Balanites aegyptiaca (L.) Del.
Authors: Sharma Priyal , Saini Mahesh Kumar , Prasad Jagdish , Gour Vinod Singh 
Published: January 2019

AbstractBalanites aegyptiaca (L.) Del. is a widely distributed xerophytic multipurpose tree. The mesocarp of the fruit of B. aegyptiaca has detergent properties due to the presence of saponins. The stability potential of this biosurfactant at varying pH, temperature, and salinity has not been explored so far. In the present study, the relative surface tensions of five different concentrations of the biosurfactant were studied at different temperatures, salinity, and under pH conditions. This study reveals that this biosurfactant retains its activity over a wide range of pH (3–11) and at high salinity (7% NaCl). It is a thermostable cationic surfactant; surfactant activity was recorded even at 100 °C with the lowest relative surface tension of 0.47. High oil displacement (18.00 mm) was observed when studied with petrol. This biosurfactant was found to have a high emulsification index (E24) of 70% with mustard oil. These results indicate that biosurfactant derived from B. aegyptiaca may find use in a wide range of sectors such as textile, food, cosmetics, oil recovery, and healthcare under a wide range of physical and chemical conditions. It offers an efficient, economically viable, and plant‐derived alternative to synthetic detergents and adds a way to maintain a sustainable environment.
Bacterial community dynamics and physicochemical characteristics in natural fermentation of jiang‐shui, a traditional food made in northwest China
Authors: Liu Zhanggen , Peng Zhen , Huang Tao , Guan Qianqian , Li Junyi , Xie Mingyong , Xiong Tao 
Published: January 2019

AbstractBACKGROUNDChinese jiang‐shui is fermented food without salt. In consideration of the few studies of the bacterial community and dynamics of jiang‐shui fermentation, the aim of this study was to investigate the microbial diversity and dynamics of jiang‐shui fermentation using high‐throughput sequencing (HTS), denaturing gradient gel electrophoresis (DGGE) and the culture‐dependent method.RESULTSThe culture‐dependent method showed that pathogenic bacteria (Staphylococcus aureus, Salmonella and Escherichia coli) and yeast mainly existed in the early stage and lactic acid bacteria dominated until the end stage. HTS and DGGE revealed that Serratia marcescens, Serratia sp., Lactobacillus curvatus, Lactococcus lactis, uncultured bacterium and Bacillus thuringiensis started the fermentation, followed by the middle stage with Lactococcus lactis, Weissella sp. and Bacillus arthracis as the predominant species. The end stage was characterized with Lactobacillus pentosus, Weissella cibaria and Weissella sp. as the major bacteria.CONCLUSIONSThe results showed that genera Serratia, Lactococcus, Weissella and Lactobacillus dominated the whole process of jiang‐shui fermentation. This study provided a good analysis of the bacterial changes of jiang‐shui fermentation, and future studies should consider the relationships among the sensory characteristics, microbial communities and metabolites. © 2019 Society of Chemical Industry