bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exploration of shared genetic susceptibility loci between type 1 diabetes and rheumatoid arthritis in the Pakistani population
Authors: Aslam Muhammad Muaaz , John Peter , Fan Kang-Hsien , Bhatti Attya , Jahangir Sidrah , Feingold Eleanor , Demirci F. Yesim , Kamboh M. Ilyas 
Published: July 2019

Abstract
Objective

Type 1 diabetes (T1D) and rheumatoid arthritis (RA) are autoimmune diseases. It is known that certain genetic loci and factors that increase the overall autoimmunity risk can be shared among different autoimmune diseases. We sought to replicate seven T1D-related SNPs that have been previously reported to be associated with RA susceptibility in a small set of mixed family-based and case-control Pakistani sample in a relatively large and independent RA case-control sample from the same population. Seven T1D-associted SNPs (GLIS3/rs7020673, BACH2/rs11755527, SKAP2/rs7804356, GDSMB/rs2290400, C6orf173/rs9388489, LOC399716/rs947474 and DLK1-MEG2/rs941576) were genotyped in a large Pakistani RA case-control sample (n=1,959) using TaqMan® SNP genotyping assays.

Results

None of the tested SNPs showed statistically significant association with RA susceptibility; however, one SNP (GLIS3/rs7020673) showed a trend for association (OR= 0.88, p=7.99E-02). Our study has failed to replicate the previously reported association of seven T1D-associted SNPs with RA risk in a large sample from the same population. Thus, our results do not support a major role of these T1D SNPs in affecting RA susceptibility in the Pakistani population.
MAPCap: A fast and quantitative transcription start site profiling protocol
Authors: Bhardwaj Vivek , Semplicio Giuseppe , Erdogdu Niyazi Umut , Akhtar Asifa 
Published: July 2019

Abstract
Below we present a simple and quick TSS quantification protocol, MAPCap (Multiplexed Affinity Purification of Capped RNA) that enables users to combine high-resolution detection of transcription start-sites and differential expression analysis. MAPCap can be used to profile TSS from dozens of samples in a multiplexed way, in 16-18 hours. MAPCap data can be analyzed using our easy-to-use software icetea (https://bioconductor.org/packages/icetea), which allows users to detect robust TSS using replicates, and perform differential TSS analysis.
Bacterial community analysis of sulfate-reducing granular sludge exposed to high concentrations of uranium
Authors: Zeng Taotao , Zhang Shiqi , Liao Wei , Ma Hualong , Lens Piet N. L. , Xie Shuibo 
Published: July 2019

Abstract
Sulfate-reducing granular sludge was used for uranium-contaminated wastewater treatment and the responsible microbial community was analyzed. Four feedings, with 6 days for every feeding and 20 mg/L initial uranium, were provided at 27.5 (±2.5) °C. After the four feedings, a uranium removal efficiency of 94% was obtained. Environmental scanning electron microscopy (ESEM) showed that short rod bacteria were the dominant microorganisms in the granular sludge. X-ray energy dispersive spectroscopy (EDS) confirmed the presence of uranium on the granular surface. High-throughput sequencing was carried out for analyzing the bacterial diversity and community structure. The total data set comprised 8,290 high quality sequences, which could be divided into 605 operational taxonomic units (OTUs). The library coverage was 0.96 and the alpha diversity indices of ACE, Chao1, Shannon and Simpson were 2,255.40, 1,346.12, 4.03 and 0.05, respectively. There were 13 bacterial genera present with a ratio of more than 1% of the total 124 genera, among which Desulfovibrio (16.48%), Clostridium IV (9.29%), Bacteroides (3.46%) and Citrobacter (1.41%) were assumed as the functional bacteria, with a cumulative proportion of 30.64% of the total bacterial population. The results provide insights into the bacterial community of sulfate-reducing granular sludge exposed to high concentrations of uranium (20 mg/L).
Preparation of sustainable non-combustion filler substrate from waterworks sludge/aluminum slag/gypsum/silica/maifan stone for phosphorus immobilization in constructed wetlands
Authors: Wang Guanghui , Gao Jingqing , Yang Rongxue , Zhang Jingshen , Guo Han , Gao Jianlei 
Published: July 2019

Abstract
In this study, an artificial wetland filler matrix capable of effectively fixing phosphorus was prepared using a non-combustion process to save energy. To evaluate the adsorption performance of this filler, adsorption experiments were performed and the phosphorus adsorption mechanism characterization was studied. An alkaline environment was found to be conducive to the increase of adsorption capacity, but excessive alkalinity was not conducive to adsorption. Static adsorption experiments showed that the phosphorus removal rate could reach 95% in the simulated phosphorus-containing wastewater after adsorption completion. The adsorption process is closely simulated by the pseudo-second-kinetic adsorption model. The isothermal adsorption experiment data were consistent with the Langmuir and the Freundlich adsorption isotherms. The characterization results showed a large number of micropores and adsorption binding sites inside and on the surface of the filler. Speciation analysis on the adsorbed phosphorus revealed that chemisorption by calcium in this filler was the dominant adsorption mechanism. The research results of this study provide the basis and reference for the development of high-efficiency phosphorus removal filler in constructed wetlands.
An iron-carbon-activated carbon and zeolite composite filter, anaerobic-aerobic integrated denitrification device for nitrogen removal in low C/N ratio sewage
Authors: Xie Yixuan , Wan Ajun , Wang Xingmin , Dong Hengjie , Wu Yunpeng 
Published: July 2019

Abstract
In this study, we use an anaerobic-aerobic integrated denitrification (Fe/C-ZACID) device with an iron-carbon-activated carbon and zeolite composite filter to remove nitrogen from simulated low carbon-nitrogen ratio (C/N) sewage. The impacts of dissolved oxygen (DO) level, hydraulic retention time (HRT), C/N and nitrate recirculation ratio on denitrification performance were studied. The results show that when HRT was 6 h, DO was 3 ± 0.1 mg/L, influent C/N was 3, and nitrate recirculation ratio was 100%, and removal rates of 95% for ammonia and 85% for total nitrogen (TN) were achieved. A beaker comparison test demonstrated that this synergistic denitrification system included heterotrophic denitrification, physicochemical denitrification, iron autotrophic denitrification and hydrogen autotrophic denitrification, etc. The Fe/C-ZACID device has a high-efficiency nitrogen removal effect for low C/N ratio sewage and strong shock resistance, which provides technical support and a theoretical basis for advanced denitrification of rural domestic sewage.
Who will appraise the arrested property of a debtor?
Authors: Korsun Tamara Ivanovna 
Published: July 2019

In the article the author pays special attention to the assessment of the debtor's property in enforcement proceedings. The presence of experts-appraisers in the law enforcement services will ensure on-time and high-quality execution of warrants of arrest, appraise and sale of property in order to pay off the alimony, taxes, loans. The author comes to the conclusion that the use of these recommendations can improve the quality of work in the appraisal of property in the course of enforcement proceedings and ensure a balance of interests of debtors, collectors, appraisers.
In Vitro Anti-Viral Effects of Small Heat Shock Proteins 20 and 27: A Novel Therapeutic Approach
Authors: Vahabpour Rouhollah , Soleymani Sepehr , Roohvand Farzin , Zabihollahi Rezvan , Bolhassani Azam 
Published: July 2019

Background:The protective effects of heat shock proteins (Hsps) were studied in some infectious and non-infectious diseases, but their specificity was slightly known in various disorders. Among Hsps, small Hsps (e.g. Hsp27 and Hsp20) have important roles in protein folding and translocation, and also in immunity.Methods:In this study, overexpression of Hsp20 and Hsp27 was performed by transfection of the plasmids encoding Hsp20 and Hsp27 (pEGFP-Hsp20 and pEGFP-Hsp27) into Huh7.5, Hela and Vero cells using Lipofectamine along with heat shock. Then, their anti-herpes simplex virus-1 (HSV-1), anti- human immunodeficiency virus-1 (HIV-1) and anti-hepatitis C virus (HCV) effects, as well as cytotoxicity, were evaluated in vitro, for the first time.Results:Our data showed that simultaneous treatment with Lipofectamine and heat shock augmented the rate of transfection and subsequently the expression of Hsps in these cells. Moreover, overexpression of Hsp20 in HCV-infected Huh7.5 cells, HIV-infected Hela cells and HSV-infected Vero cells reduced the replication of HCV, HIV and HSV, respectively. In contrast, overexpression of Hsp27 significantly decreased HSV replication similar to Hsp20, but it did not affect the replication of HIV and HCV.Conclusion:Generally, Hsp20 was identified as a novel anti-HCV, anti-HSV and anti-HIV agent, but Hsp27 was efficient in the suppression of HSV infection. These Hsps may act through suppression of virus entry and/ or through interaction with viral proteins. Thus, it is necessary to determine their exact mechanisms in the near future.
Effect of Levetiracetam on Cognitive Function and Clonic Seizure Frequency in Children with Epilepsy
Authors: Zhou Shihao , Zhan Qiong , Wu Xiaomei 
Published: July 2019

Background:
This study aimed to explore the clinical effect of levetiracetam in
the treatment of children with epilepsy.

Methods:
136 children with epilepsy were selected from January 2017 to December
2017. According to the random number table method, they were divided into the
experimental group and the conventional group, with 68 cases in each group. The
conventional group was treated with valproate, while the experimental group was treated
with levetiracetam. The effective rate, the cognitive function and the frequency of clonic
seizures in the two groups were compared.

Results:
There was no significant difference in the total effective rate between the two
groups (P>0.05). There was no significant difference in attention, executive ability,
abstract and orientation scores between the two groups before treatment (P>0.05). After
treatment, the focus of attention (106.54±6.56), executive ability (105.76±6.77), abstract
and directional score (106.65±6.57) were significantly higher than that of the
conventional group. The difference in the two groups was statistically significant
(P<0.05). After 3 months of treatment, the frequency of myoclonic seizures (9.22±0.95)
and the frequency of tonic-clonic seizures (11.68±1.36) were found to be significantly
lower than those of the conventional group, and the difference between the two groups
was statistically significant (P<0.05).

Conclusion:
Levetiracetam is effective in the treatment of children with epilepsy. It can
effectively improve the cognitive function of the patients, reduce the frequency of
myoclonic seizures and tonic-clonic seizures, and has a high promotion value.
Quercetin Promotes Cell Cycle Arrest and Apoptosis and Attenuates the Proliferation of Human Chronic Myeloid Leukemia Cell Line-K562 Through Interaction with HSPs (70 and 90), MAT2A and FOXM1
Authors: Hassanzadeh Ali , Hosseinzadeh Elham , Rezapour Saleheh , Vahedi Ghasem , Haghnavaz Navideh , Marofi Faroogh 
Published: July 2019

Background:
Chronic Myeloid Leukaemia (CML) starts in certain blood-forming cells of the bone
marrow when cells acquire Philadelphia chromosome. Nowadays, scientists attempt to find novel and safe
therapeutic agents and approaches for CML therapy using Tyrosine Kinase Inhibitors (TKIs), CML
conventional treatment agents, has some restrictions and also adverse effects. Recently, it has been proposed that
phytochemicals, such as flavonoids due to their low side effects and notable safety have the potential to be used
for CML therapy.

Materials and Methods:
K-562 cells were exposed with three concentrations of the querectin (10, 40 and
80µM) for 12, 24 and 48 hours. After that, these cells apoptosis rate was estimated using Annexin-V/PI staining
and flowcytometry analysis, and their proliferation rate was evaluated using 3-[4,5-dimethylthiazol-2-yl]-2,5
diphenyl tetrazolium bromide (MTT). Finally, the expression of the 70 and 90 kilodalton heat shock proteins
(HSP70 and 90), methionine adenosyltransferase 2A (MAT2A), Forkhead box protein M1 (FOXM1), caspase-3
and -8, Bcl-X(L) and Bax involved in leukemic cells survival and proliferation was assessed using Real-Time
PCR within 12, 24 and 48 hours after exposure with quercetin 40 and 80µM.

Results:
Considering consequences, querecetin induced apoptosis in K-562 cells, and also abrogated these cells
proliferation. On the other hand, RT-PCR results showed a reduction in some of the candidate genes expression,
especially HSP70, Bcl-X(L) and FOXM1, when cells were treated with quercetin 40 and 80µM. Also, Bax,
caspase-3 and caspase-8 expression was significantly improved in K-562 cells upon quercetin exposure.

Conclusion:
We concluded that CML therapy by querecetin due to its anti-proliferative and anti-survival
potentials could lead to the promising therapeutic outcome through targeting major survival and proliferation
involved genes expression.
Tipificación de sistemas de producción de café en La Unión Nariño, Colombia
Authors: Ordóñez Jurado Héctor Ramiro , Navia Estrada Jorge Fernando , Ballesteros Possú William 
Published: July 2019

En Colombia, la diversidad de agroecosistemas le genera al café sabores y aromas característicos. Un primer paso para conocer los aspectos particulares de cada zona es la descripción de los sistemas productivos. En la zona cafetera del municipio de la Unión Nariño, localizada a 1º 36’ y 06’’ de latitud Norte y a 77º 00’ y 15’’ longitud Oeste, la actividad cafetera se desarrolla en fincas productivas heterogéneas en términos de extensión, distancias de siembra, tecnología aplicada, organización, estructura familiar y fundamentalmente en el uso y manejo de la sombra; razón por la cual, es importante tipificarlos mediante la integración de la dimensión social, económica y ambiental y establecer las diferencias entre ellos, conocer su dinámica y determinar las potencialidades y las limitantes para contribuir a los procesos de planificación, investigación, y organización del sector cafetero. Se aplicó una encuesta semiestructurada a 104 productores ubicados entre los 1400 y 1800 msnm. Esta información se complementó con información proveniente de las instituciones vinculadas al sector cafetero. Un análisis multivariado a partir de un análisis de conglomerados (método de Ward y de componentes principales), fue aplicado a las variables con alta correlación. Se establecieron cuatro tipologías de sistemas de producción: café con sombra de leñosas (S1), café semi-sombra (S2), café- plátano (S3) y el sistema de cultivo café sin sombra (S4). Al interior de cada tipología, se encontraron diferentes dinámicas en las prácticas de manejo del cultivo, conllevando a diferencias en la producción, calidad y rendimientos económicos
Review – New Media Caucus Showcase A perspective on consciousness within digital practices
Authors: Faccion Debora 
Published: July 2019

Presentations by the following NMC members:

Sophia Brueckner, Assistant Professor, University of Michigan
Martin Calvino, Artist-in-residence at Rutgers University
Zach Duer, Assistant Professor, Creative Technologies, Virginia Tech
Carter Eggleston, MFA candidate in Creative Technologies, Virginia Tech
Elizabeth Flyntz, Artist, Writer, and curator
Jeffrey Gangwisch, strikeWare co-founder and Adjunct Professor, Anne Arundel Community College
Chelsea Heikes, candidate at European Graduate School
Laura Hyunjhee Kim, Ph.D. candidate, University of Colorado Boulder
George Legrady, Distinguished Professor of Interactive Media and director of Experimental Visualization Lab, UC Santa Barbara
Maya Livio, Ph.D Candidate, University of Colorado Boulder, Curator of Media Archaeology Lab, Curator of MediaLive at BMoCA
Eric Souther, Associate Professor of New Media, Indiana University South Bend
Deirtra Thompson, Independent artist, practitioner, and researcher
Masha Vlasova, Lecturer and Lead Faculty, University of North Texas
Michelle Hernandez, MFA alumna, Hunter College, CUNY
Dominika Ksel, Adjunct Assistant Professor of New Media, Baruch College, CUNY.
Presymptomatic Screening for Intracranial Aneurysms in Patients with Autosomal Dominant Polycystic Kidney Disease
Authors: Sanchis Irina M. , Shukoor Shehbaz , Irazabal Maria V. , Madsen Charles D. , Chebib Fouad T. , Hogan Marie C. , El-Zoghby Ziad , Harris Peter C. , Huston John , Brown Robert D. , Torres Vicente E. 
Published: July 2019

Background and objectives
Intracranial aneurysm rupture is the most devastating complication of autosomal dominant polycystic kidney disease. Whether selective or widespread intracranial aneurysm screening is indicated remains controversial.

Design, setting, participants & measurements
Records of 3010 patients with autosomal dominant polycystic kidney disease evaluated at the Mayo Clinic between 1989 and 2017 were reviewed. Those who had presymptomatic magnetic resonance angiography screening were included.

Results
Ninety-four intracranial aneurysms were diagnosed in 75 of 812 (9%) patients who underwent magnetic resonance angiography screening. Sex, age, race, and genotype were similar in the groups with and without aneurysms; hypertension and history of smoking were more frequent in the aneurysm group. Twenty-nine percent of patients with aneurysms compared with 11% of those without aneurysms had a family history of subarachnoid hemorrhage (P<0.001). Most aneurysms were small (median diameter =4 mm; range, 2–12 mm); 85% were in the anterior circulation. During a total imaging follow-up of 469 patient-years, de novo intracranial aneurysms were detected in five patients; eight intracranial aneurysms grew (median =2 mm; range, 1–3 mm). During a total clinical follow-up of 668 patient-years, seven patients had preemptive clipping or coil embolization; no intracranial aneurysms ruptured. During a total clinical follow-up of 4783 patient-years in 737 patients with no intracranial aneurysm detected on the first magnetic resonance angiography screening, two patients had an intracranial aneurysm rupture (0.04 per 100 person-years; 95% confidence interval, 0 to 0.10). The rate of intracranial aneurysm rupture in large clinical trials of autosomal dominant polycystic kidney disease was 0.04 per 100 patient-years (95% confidence interval, 0.01 to 0.06).

Conclusions
Intracranial aneurysms were detected by presymptomatic screening in 9% of patients with autosomal dominant polycystic kidney disease, more frequently in those with familial history of subarachnoid hemorrhage, hypertension, or smoking. None of the patients with and two of the patients without aneurysm detection on screening suffered aneurysmal ruptures. The overall rupture rate in our autosomal dominant polycystic kidney disease cohort was approximately five times higher than that in the general population.
Conocimiento y percepciones médicas sobre la fisioterapia y el trabajo interdisciplinario
Authors: Estrada-Gómez Cyntia Gabriela , Gutiérrez Camacho Claudia , Salinas Sánchez Igor , Peñaloza Ochoa Laura 
Published: July 2019

Introducción: Las actividades del fisioterapeuta incluyen atención clínica, gestión, docencia, investigación e implementación de políticas en salud. Desafortunadamente, el conocimiento médico del involucramiento del fisioterapeuta en estas actividades es precario, dificultando el trabajo interdisciplinario.
Objetivo: Describir el conocimiento y percepción de los médicos residentes de un hospital de tercer nivel sobre las actividades e importancia del trabajo interdisciplinario con la fisioterapia.
Método: Estudio observacional, descriptivo, de corte transversal. Se construyó y aplicó un cuestionario de 12 reactivos de opción múltiple en residentes de pediatría, genética médica, subespecialidades pediátricas y cursos de alta especialidad en Medicina, quienes respondieron al menos el 80% de las preguntas. Se excluyeron residentes cuyos pacientes reciben fisioterapia en forma cotidiana.
Resultados: Se incluyeron 158 médicos residentes, el 77.8% conocía sólo las actividades clínicas del fisioterapeuta. El 68% solicitó entre 1 a 4 interconsultas al mes. La mayoría reconoció la autonomía del fisioterapeuta para establecer diagnósticos funcionales más no tratamientos (68%), más de la mitad (66.7%) señaló la necesidad de pases de visita, sesiones clínicas y entregas de guardia en conjunto.
Conclusiones: Sería conveniente incrementar el currículo del médico en formación sobre el campo de acción del fisioterapeuta y los beneficios del trabajo interdisciplinario.
El médico residente de urología como educador. Una intervención educativa participativa
Authors: Leyva González Félix Arturo , Leyva Salas César Arturo , F. Gómez Clavelina Francisco Javier , Leyva Salas Félix Arturo , Valencia Sánchez Jesús Salvador 
Published: July 2019

Objetivo: Determinar el alcance de una intervención educativa promotora de la participación –implementada por un médico residente de quinto año– con respecto al desarrollo de la aptitud clínica de médicos residentes de urología, en la atención de los cuatro padecimientos urológicos más frecuentes, en un tercer nivel de atención médica.
Método: Se realizó un estudio de intervención en médicos residentes de segundo (R2 n = 7) y tercer año (R3n = 6) de urología en Centro Médico Nacional “La Raza”, en la Ciudad de México, México, periodo: noviembre - diciembre de 2017. Se construyó un instrumento que evaluó la variable: “aptitud clínica” de médicos residentes de segundo y tercer año, en cuatro padecimientos urológicos; la validez conceptual, de contenido y confiabilidad, se valoró por cuatro médicos especialistas en urología, con experiencia en docencia e investigación educativa. La intervención educativa fue implementada por un residente de urología de quinto año. La intervención educativa se desarrolló en forma de seminarios, los cuales se realizaron dos veces por semana (lunes y viernes); duración por sesión: dos horas; duración total de la intervención educativa: 16 sesiones. El instrumento de medición se aplicó al inicio (1ª sesión) y al finalizar la intervención educativa (16ª sesión).
Resultados: En los R2 y R3 se observaron avances en todos los indicadores de aptitud clínica y en la calificación desaglobal, lo cual se expresó con diferencias estadísticamente significativas; los R2, en la calificación global (medición inicial vs. final) mostraron las siguientes medianas: 44 y 110 (p 0.018); los R3, la calificación global mostró: 52.5 y 128 (p 0.027).
Conclusiones: Una intervención educativa participativa implementada por un médico residente de urología de quinto año, se puede asociar con un avance en el desarrollo de la aptitud clínica de R2 y R3 de la misma especialidad.