bluuebunny/crossref_metadata_2025_split huggingface dataset:
The depletion of gut microbiome impairs the beneficial effect of Gui-Shen-Wan in restoring mice ovarian function and associated protein expression of ovarian tissues
Authors: Huang Xingtao , Xu Ruinan , Yang Qin , Jiang Xin , Lin Jinju , Zhao Huashan , Wu Ruifang , Du Hui , Dai Wenkui 
Published: November 2024

IntroductionTraditional Chinese Medicine (TCM), specifically Gui-Shen-Wan, has shown promise in restoring ovarian function among reproductive-age women who had impaired ovarian functions, yet the underlying mechanisms remain elusive. Recent studies highlight the pivotal role of the gut microbiome (GM) in mediating the therapeutic effects of TCM. However, it is unclear whether the GM contributes to Gui-Shen-Wan’s therapeutic restoration of ovarian functions.MethodsThis study employed a mouse model with cyclophosphamide-induced decreased ovarian function (P_T and P_AT groups) and a control group without modeling. The P_AT group received a 7-day course of oral antibiotics to deplete the GM prior to a 20-day Gui-Shen-Wan treatment regimen.ResultsBoth P_T and P_AT mice exhibited prolonged metestrus/diestrus phases compared to controls (p&lt;0.05), indicating menstrual disruption post-modeling. Following 20 days of Gui-Shen-Wan treatment, P_T mice showed a shorter metestrus/diestrus phase (4 days) compared to P_AT mice (5 days) (p&lt;0.05). Notably, P_T mice had a higher number of normal follicles(primitive/primary/secondary/antral follicles) in their ovaries post-treatment (median 15) compared to P_AT mice (median 8.5). Proteome analysis revealed that ovarian proteins enriched in P_T mice were primarily associated with oxidative phosphorylation and DNA replication pathways, suggesting GM-mediated enhancement of these processes.DiscussionThis study underscores the pivotal role of the GM in the therapeutic benefits of Gui-Shen-Wan, highlighting the potential for microbiome-targeted interventions in promoting beneficial effects of Gui-Shen-Wan on the restoration of decreased ovarian functions.
How does innovative customer knowledge influence electronic word-of-mouth recommendation behavior through egoistic and altruistic approaches? Testing a moderated mediation model
Authors: Qi Xiaobo 
Published: November 2024

IntroductionAt present, innovative customer knowledge on social media platforms is mostly treated as a kind of “self-entertainment” activity content, and has not been effectively integrated and used by sales-oriented social media enterprises. As a result, the problem of electronic word-of-mouth recommendation reducing for such enterprises is more prominent. Theoretically, on the one hand, researches in the field of customer knowledge are mainly concentrated in the field of knowledge management led by the enterprise, which lacks to discuss the influence of innovative knowledge on electronic word-of-mouth recommendation behavior from the perspective of customer dominance. On the other hand, previous studies lack to explore the formation mechanism of electronic word-of-mouth recommendation behavior of innovative customers from the dual paths of altruism and egoism. The professional characteristics and social characteristics of innovative customers are not analyzed as boundary conditions. In this context, based on social learning theory and social identity theory, this study explores the influence mechanism of innovative customer knowledge on electronic word-of-mouth recommendation behavior.MethodsData were collected by Likert 5-level scale using questionnaire survey method. A total of 452 valid questionnaires were collected from Northeast, Northwest, East, North, Central, South and southwest China by random sampling method. SPSS21.0 software and Bootstrap (PROCESS3.0) program were used to analyze the multilevel linear regression, structural equation model and robustness test of the model.Results(1) Innovative customer knowledge had a significant positive impact on electronic word-of-mouth recommendation behavior and professional identity; (2) Professional identity had a significant positive effect on electronic word-of-mouth recommendation behavior; (3) Professional identity played mediating role between innovative customer knowledge and electronic word-of-mouth recommendation behavior; (4) Compared with high professional status and high social status, low professional status and low social status had a stronger moderating effect on the relationship between professional identity and electronic word-of-mouth recommendation behavior, and there was a moderated mediation effect.DiscussionInnovative customer knowledge generates electronic word-of-mouth recommendation behavior through two paths: altruism and egoism. These two paths are affected by the external characteristics of innovative customers, that is, professional characteristics and social characteristics have a moderating effect on them.
The impact of freshman learning communities on students’ academic performance
Authors: Elobaid Manal , Zidani Mounia , Koffa Nesreen , Qadi Saba 
Published: November 2024

IntroductionThe global issue of first-year student retention is a significant concern, generally associated with poor academic performance resulting from insufficient social and intellectual integration. Freshman Learning Communities (FLC) have arisen as a promising approach to tackle these difficulties. This study aims to assess the influence of FLC on the scholastic achievement of first-year undergraduate students.MethodsThe study utilizes quantitative research to determine if involvement in FLC has a favorable impact on academic performance by comparing the performance of students participating in FLC to those who are not part of the program. Descriptive and inferential statistical analyses, including t-tests and ANCOVA, were employed to identify significant differences between the two groups.ResultsSignificant differences were identified, with findings indicating that students participating in the FLC exhibit superior academic performance, reflected in higher grade point averages (GPA). No significant difference was observed in registered and earned credit hours between FLC participants and non-participants.DiscussionThe results suggest that FLC participation is associated with improved academic performance, supporting FLC as a potential strategy to enhance scholastic achievement among first-year students.
Mobile health (mHealth) interventions for health promotion during the perinatal period in India: a scoping review
Authors: Small Zara , Thompson Sophie Elizabeth , Sharma Ankita , Majumdar Sreya , Thout Sudhir Raj , Praveen Devarsetty , Hirst Jane Elizabeth 
Published: November 2024

IntroductionPerinatal and maternal mortality rates remain high in India compared to global levels, and there is significant heterogeneity in outcomes across Indian states. Many mobile health (mHealth) interventions have been developed to improve maternal and infant health outcomes in India, however it is unclear how mHealth can best support women in this culturally and resource diverse setting. Therefore, we aimed to identify mHealth interventions targeting women and their families in the perinatal period in India, identify barriers and facilitators to their uptake, and future research directions.MethodsThe Preferred Reporting Items for Systematic Reviews and Meta-Analyses and Joanna Briggs Institute guidelines for scoping reviews was used for study selection and screening and the mHealth evidence reporting and assessment checklist was used for evaluating mHealth interventions. PubMed, CINAHL, Global Health, and ACM digital library were searched for records up to 2 April 2023. Studies were included where women who were pregnant, planning for a child, or in the 12 months after delivery, and their families, living in India received health advice via a technological medium.Results1,783 records were screened, 29 met the inclusion criteria, describing 22 different mHealth interventions. Most frequent behavioural targets for interventions were breastfeeding, antenatal nutrition, and infant healthcare. Most interventions communicated to women through one-way communication methods, most frequently SMS. Participants reported positive views of mHealth, reported facilitators included group communication, use of non-maternal informative content, and a pictorial information format. Reported barriers included household responsibilities, technical difficulties, difficulty accessing a phone and difficulty understanding, or misinterpreting messages.DiscussionWe conclude that mHealth interventions are acceptable to women in India during the perinatal period. However, current interventions lack evidence of long term behavioural change and fail to report on features important in sustainability and scalability, namely network infrastructure, data security and interoperability. We propose the need for a framework to understand existing cultural beliefs and support structures to avoid early intervention failure. Future research should investigate multimodal mHealth interventions for behavioural change, identify the appropriate frequency and format of mHealth messages, and address access limitations such as shared mobile phone ownership, and illiteracy rates.
Prevalence and determinants of termination of pregnancy among reproductive-age women who had a short preceding birth interval in Sub-Saharan Africa: a multilevel analysis
Authors: Zegeye Alebachew Ferede , Tamir Tadesse Tarik , Mekonen Enyew Getaneh , Techane Masresha Asmare , Terefe Bewuketu , Workneh Belayneh Shetie 
Published: November 2024

BackgroundTermination of pregnancy is one of the biggest five causes of maternal mortality in countries with low and middle incomes. Although termination of pregnancy is hazardous, its prevalence and determinates are not well studied in developing countries. Therefore, this study aims to assess the prevalence and determinants of termination of pregnancy among reproductive-age women who had a short preceding birth interval in Sub-Saharan Africa.MethodsData from the most recent Demographic and Health Surveys, which covered 21 Sub-Saharan African countries from 2015 to 2022, were used for secondary data analysis. The study used a total of 283,785 women. Stata 14 was used to analyze the data. The determinants of termination of pregnancy were determined using a multilevel mixed-effects logistic regression model. Significant factors associated with termination of pregnancy were declared significant at p-values &lt; 0.05. The result was interpreted using the confidence interval and adjusted odds ratio. The best-fit model was determined to be the one with the highest log likelihood ratio and the lowest deviance.ResultsIn Sub-Saharan Africa, one in ten women with short birth intervals experienced pregnancy termination. Individual factors, including the sex of the preceding birth (AOR = 1.21, 95% CI: 1.05, 1.40), maternal age (AOR = 1.57, 95% CI: 1.27, 1.95), pregnancy complications (AOR = 1.28, 95% CI: 1.09, 1.49), No ANC visits (AOR = 2.29, 95% CI: 1.26, 4.14), previous cesarean section delivery (AOR = 1.74, 95% CI: 1.32, 2.30), &lt;6 months of breastfeeding (AOR = 1.56, 95% CI: 1.35, 1.81), traditional contraception usage (AOR = 1.67, 95% CI: 1.13, 2.46), poor wealth status (AOR = 1.50, 95% CI: 1.22, 1.85), and community-level factors such as urban residence (AOR = 1.31, 95% CI: 1.06, 1.62) had higher odds of pregnancy termination.ConclusionsThis study concludes that termination of pregnancy rates among women with short preceding birth interval is high. The study identified that both individual and community-level variables were determinants of termination of pregnancy. Therefore, the ministries of health in Sub-Saharan African countries should give attention to those women who underutilize antenatal care services and to women from urban areas while designing policies and strategies targeting reducing termination of pregnancy rates.
Cutibacterium acnes strains associated with bone prosthesis infections cannot evade the host immune system
Authors: Thoraval Léa , Tang-Fichaux Min , Guillaume Christine , Varin-Simon Jennifer , Dumortier Claire , Sergheraert Johan , Lamret Fabien , Bonhomme Mélanie , Laurent Frédéric , Josse Jérôme , Gangloff Sophie C. , Mongaret Céline , Reffuveille Fany , Velard Frédéric 
Published: November 2024

IntroductionCutibacterium acnes is a commensal skin bacterium that is involved in bone prosthesis infections (BPIs) and presents low-grade clinical symptoms. C. acnes has been thought to escape the immune system at bone sites.Material and methodsOur study was carried out on a laboratory strain and two BPI-related clinical strains, one of which surprisingly induced clinical symptoms of inflammation in the patient. We investigated the ability of these C. acnes strains to trigger in vitro human primary neutrophils (PMN) response through inflammatory mediators measurements (antibody arrays, ELISA, RT-qPCR, zymography) and activation status assessment (flow cytometry), and to induce in vivo PMN recruitment from the bloodstream in mice air-pouch model. PMN-mediated inflammation was also studied in an original in vitro model mimetic of an infected bone site that combine titanium alloy, human primary osteoblasts, human primary neutrophils and C. acnes strains.ResultsWe demonstrated for the first time that both C. acnes planktonic and biofilm cultures, triggered an effective immune response by neutrophils in vitro and their recruitment in vivo. This host response was enhanced when using a strain from a patient with inflammatory signs. In an original infected prosthesis mimetic model, osteoblasts and neutrophils were able to detect C. acnes, but their response to the clinical C. acnes inflammatory strain decreased.ConclusionThis work provides the first evidence showing that the immune cell response to pathogenic C. acnes may be tuned by nonimmune cells at the infected site, such as osteoblasts, which may promote bacterial persistence.
Extracellular vesicles epitopes as potential biomarker candidates in patients with traumatic spinal cord injury
Authors: Hörauf Jason-Alexander , Schindler Cora Rebecca , Schaible Inna , Wang Minhong , Weber Birte , El Saman André , Pallas Christiane , Widera Marek , Marzi Ingo , Henrich Dirk , Leppik Liudmila 
Published: November 2024

BackgroundExtracellular vesicles (EVs), a heterogeneous group of cell-derived, membrane-enclosed vesicles bearing cell-specific epitopes, have been demonstrated to play a crucial role in neuronal-glial communication and the orchestration of neuroinflammatory processes. However, the existing evidence regarding their function as biomarkers and their role in the pathobiology of traumatic spinal cord injuries (tSCI), particularly in humans, is scarce.ObjectiveThe primary goal of this study was to investigate whether a distinct pattern of EV surface epitopes detected in the plasma of individuals suffering from spinal cord injury is indicative of tSCI.MethodsThe study includes patients with isolated tSCI (n=8), polytrauma patients without tSCI (PT; ISS ≥16, n=8), and healthy volunteers (HV; n=8). Plasma samples from tSCI and PT patients were collected right after admission to the emergency room (ER), 24 hours (24h), and 48h after trauma. EVs were isolated via size exclusion chromatography, and EVs’ surface epitopes were quantified with MACSPlex EV Kit Neuro (prototype product, Miltenyi Biotec) and compared among the groups. Additionally, results were correlated with clinical parameters.ResultsIn total, 19 epitopes differed significantly between the tSCI and the HV groups. Out of these 19, four (CD47, CD56, CD68, and ADAM17) were found to differ significantly among tSCI and PT groups. The expression of the CD47 epitope was found to correlate positively with the American Spinal Injury Association (ASIA) impairment scale.ConclusionWe identified four potential EV-based tSCI biomarkers (CD47+, CD56+, CD68+, and ADAM17+ EVs) that differ in tSCI, with CD47+ EVs showing a strong correlation with the neurological function in tSCI. Thus, future studies might further specify the relevance of potential tSCI-specific biomarkers and investigate underlying mechanisms of tSCI.
The load of hepatitis B virus reduces the immune checkpoint inhibitors efficiency in hepatocellular carcinoma patients
Authors: Ji Zhengzheng , Li Jiasong , Zhang Shasha , Jia Yuanyuan , Zhang Jing , Guo Zhanjun 
Published: November 2024

IntroductionChronic viral infection may lead to an immunosuppressive microenvironment, whereas the association between virus-related indicators and treatment response in hepatocellular carcinoma(HCC) patients undergoing immune checkpoint inhibitors(ICIs) therapy remains a topic of debate. We aim to investigate the influence of hepatitis virus on the ICI efficiency in HCC patients through a meta-analysis.MethodsWe searched PubMed, Cochrane Library, Embase, and Web of Science until 14 July 2024 to identify cohort studies involving ICIs treatments in HCC patients. We extracted data from the literature related to hepatitis B virus (HBV) infection, hepatitis C virus (HCV) infection, baseline HBV load, and antiviral therapy. Overall survival (OS) and progression-free survival (PFS) were considered as the primary endpoints, while objective response rate (ORR) was regarded as a secondary endpoint.ResultsWe included 55 cohort studies published between 2019 and 2024, involving a patient population of 7180 individuals. Summarized hazard ratio (HR) comparing HBV infection with non-HBV infection in the context of ICIs therapy revealed no significant association between HBV infection and either mortality risk or progression risk with the pooled HR for OS of 1.04(95%CI: 0.93-1.16, P=0.483) and the pooled HR for PFS of 1.07(95%CI:0.96-1.20, P=0.342). HBV infected patients with HCC may have better tumor response than non-HBV infected patients receiving ICIs with the combined relative risk(RR) for ORR was 1.94 (95%CI: 1.12-3.38, P=0.002). High baseline HBV load is associated with poor survival outcomes in patients with HCC who receive ICIs with the pooled HR for OS was 1.74 (95%CI: 1.27-2.37, P=0.001), thereby antiviral therapy has the potential to significantly enhance prognostic outcomes with the pooled HR for OS was 0.24 (95% CI: 0.14-0.42 P&lt;0.001) and the pooled HR for PFS was 0.54 (95% CI: 0.33-0.89 P=0.014).ConclusionIn individuals with HCC who received ICIs, there was no notable link found between HBV or HCV infection and prognosis. However, HBV infection showed a connection with improved tumor response. A higher initial HBV load is linked to worse survival results in HCC patients undergoing ICIs treatment and antiviral therapy can significantly improve its prognosis.
Machine learning to predict radiomics models of classical trigeminal neuralgia response to percutaneous balloon compression treatment
Authors: Wu Ji , Qin Chengjian , Zhou Yixuan , Wei Xuanlei , Qin Deling , Chen Keyu , Cai Yuankun , Shen Lei , Yang Jingyi , Xu Dongyuan , Chai Songshan , Xiong Nanxiang 
Published: November 2024

BackgroundClassic trigeminal neuralgia (CTN) seriously affects patients’ quality of life. Percutaneous balloon compression (PBC) is a surgical program for treating trigeminal neuralgia. But some patients are ineffective or relapse after treatment. The aim is to use machine learning to construct clinical imaging models to predict relapse after treatment (PBC).MethodsThe clinical data and intraoperative balloon imaging data of CTN from January 2017 to August 2023 were retrospectively analyzed. The relationship between least absolute shrinkage and selection operator and random forest prediction of PBC postoperative recurrence, ROC curve and decision -decision curve analysis is used to evaluate the impact of imaging histology on TN recurrence.ResultsImaging features, like original_shape_Maximum2D, DiameterRow, Original_Shape_Elongation, etc. predict the prognosis of TN on PBC. The areas under roc curve were 0.812 and 0.874, respectively. The area under the ROC curve of the final model is 0.872. DCA and calibration curves show that nomogram has a promising future in clinical application.ConclusionThe combination of machine learning and clinical imaging and clinical information has the good potential of predicting PBC in CTN treatment. The efficacy of CTN is suitable for clinical applications of CTN patients after PBC.
Effect of histone demethylase KDM5B on long-term cognitive impairment in neonatal rats induced by sevoflurane
Authors: Wang Yanhong , Chen Yun , Zhang Mengxiao , Yuan Chengdong , Zhang Yu , Liu Xingjian , Zhang Yi , Liang Xiaoli 
Published: November 2024

IntroductionWhether repeated inhalation of sevoflurane during the neonatal period causes long-term learning and memory impairments in humans is unclear. Some recent investigations have indicated that general anesthesia drugs affect histone methylation modification and may further affect learning and memory ability. This study aimed to explore the role and mechanism of histone methylation in long-term cognitive dysfunction caused by repeated inhalation of sevoflurane during the neonatal period.MethodsNeonatal SD rats were assigned into three groups. Sevoflurane group and sevoflurane +AS8351 group were exposed to 2% sevoflurane for 4 h on postnatal day 7 (P7), day 14 (P7) and day 21 (P21), and the control group was inhaled the air oxygen mixture at the same time. From postnatal day 22 to 36, rats in the +AS8351 group were treated with AS8351 while those in the Sevoflurane group and control group were treated with normal saline. Half of the rats were carried out Y-maze, Morris water maze (MWM), western blot and transmission electron microscope at P37, and the remaining rats were fed to P97 for the same experiment.ResultsNeonatal sevoflurane exposure affected histone demethylase expression in hippocampus, changed histone methylation levels, Down-regulated synapse-associated protein expression, impaired synaptic plasticity and long-term cognitive dysfunction and KDM5B inhibitors partially restored the negative reaction caused by sevoflurane exposure.DiscussionIn conclusion, KDM5B inhibitor can save the long-term learning and memory impairment caused by sevoflurane exposure in neonatal period by inhibiting KDM5B activity.
Effect of chewing hard material on boosting brain antioxidant levels and enhancing cognitive function
Authors: Kim Seungho , Kim Ji-Hye , Lee Hansol , Jang Sung Ho , Noeske Ralph , Choi Changho , Chang Yongmin , Choi Youn-Hee 
Published: November 2024

IntroductionChewing has been reported to enhance cognitive function through the increase in cerebral blood flow. However, the mechanisms linking cerebral blood flow increase to metabolic changes in the brain affecting cognition remain unclear. We hypothesized that glutathione (GSH) plays a pivotal role in these mechanisms. Therefore, this study aimed to investigate changes in brain GSH levels following chewing and their association with cognitive function in healthy young adults.MethodsA total of 52 university students were recruited, and the Korean version of the Repeatable Battery for the Assessment of Neuropsychological Status was used for the neurocognitive evaluations. Brain GSH levels following chewing gum or wood blocks were measured using MEscher-GArwood Point RESolved Spectroscopy (MEGA-PRESS) sequence, and their relevance to neurocognitive evaluation results was investigated.ResultsChewing significantly increased brain GSH concentration, particularly in the wood-chewing group compared to the gum-chewing group, as observed in the anterior cingulate cortex. Furthermore, the rise in GSH concentration in the wood-chewing group was positively correlated with memory function.ConclusionChewing moderately hard material elevates brain antioxidant levels such as GSH, potentially influencing cognitive function.