bluuebunny/crossref_metadata_2025_split huggingface dataset:
In vivo identification of GTPase interactors by mitochondrial relocalization and proximity biotinylation
Authors: Gillingham Alison , Bertram Jessie , Begum Farida , Munro Sean 
Published: February 2019

AbstractThe GTPases of the Ras superfamily regulate cell growth, membrane traffic and the cytoskeleton, and a wide range of diseases are caused by mutations in particular members. They function as switchable landmarks with the active GTP-bound form recruiting to the membrane a specific set of effector proteins. The GTPases are precisely controlled by regulators that promote acquisition of GTP (GEFs) or its hydrolysis to GDP (GAPs). We report here MitoID, a method for identifying effectors and regulators by performing in vivo proximity biotinylation with mitochondrially-localized forms of the GTPases. Applying this to 11 human Rab GTPases identified many known effectors and GAPs, as well as putative novel effectors, with examples of the latter validated for Rab2, Rab5 and Rab9. MitoID can also efficiently identify effectors and GAPs of Rho and Ras family GTPases such as Cdc42, RhoA, Rheb, and N-Ras, and can identify GEFs by use of GDP-bound forms.
Modeling and Visualization of Rice Root Based on Morphological Parameters
Authors: Yang Le , Shao Peng 
Published: February 2019

AbstractTo clarify the morphological distributional characteristic of rice roots, the “root box” experiments are conducted to extract various morphological parameters of roots. On the basis of experiments, in this paper, the rice root model based on morphological parameters is constructed with B-spline curves by analyzing the topological structure of rice roots, quantifying their biological characteristics, summarizing the morphological structure and growth characteristics and improving the Cubic growth function to describe the growth change of rice roots. Meanwhile, the output accuracy of the model is tested. Finally, the dynamic simulation of rice root growth characteristics in three-dimensional space is implemented by using Visual C++ and OpenGL standard graphics library. The compared results demonstrate that the model could faithfully simulate the dynamic growing process of rice roots, and help to enrich the methods of digitization and visualization for roots of other crops.
Validation of an agent-specific safety climate model for construction
Authors: Newaz Mohammad Tanvi , Davis Peter Rex , Jefferies Marcus , Pillay Manikam 
Published: February 2019

Purpose
Safety climate (SC) is considered a leading indicator of safety performance, but scholars suggest that a common SC assessment framework is yet to be developed. Following the debate between the importance of facet analysis and agent analysis, the purpose of this paper is to test a factor structure, developed by the authors in previous work and arising from their systematic literature review, highlighting the role of safety agents in a construction site setting.

Design/methodology/approach
Multi-level SC surveys were conducted at five construction sites in Sydney, Australia, collecting data from of 352 workers associated with a mega-construction project. While examining the factor analysis of different studies, data reliability and data validity of the survey findings were ensured and a goodness-of-fit of SC model was examined through structural equation modelling.

Findings
The systematic literature review of Newaz et al. (2018) suggested a five-factor model of: management commitment, safety system, role of the supervisor, workers’ involvement and group SC. However, empirical data indicated that the questionnaire used to measure “safety system” failed to pass scale reliability; thus, a four-factor model was proposed to develop an agent-specific SC factor structure in the construction industry.

Originality/value
The four-factor model indicates the role and level of influence of different safety agents to improve safety perceptions on construction sites. The findings of this study will encourage researchers in construction safety to use the simplified four-factor SC (agent-specific) model presented and test it to further develop a common factor structure for the construction industry. The fact that the model is comprised of four factors makes further implementation somewhat easier in the development of safety plans, and when considering the role of safety agents, therefore enhancing its potential value.
Predicting Users’ willingness to diffuse healthcare knowledge in social media
Authors: Jin Xiao-Ling , Zhou Zhongyun , Yu Xiaoyu 
Published: February 2019

Purpose
The purpose of this paper is to investigate why users are willing to diffuse healthcare knowledge in social media by drawing on the communicative ecology theory (CET) and prior research on interpersonal communication.

Design/methodology/approach
This paper conducts a large-scale scenario-based online survey in WeChat (the most popular social media platform in China) to test the proposed research model and hypotheses. The final data set consists of 1,039 useful responses from WeChat users.

Findings
The results indicate that interestingness, emotionality and institution-based trust are the strongest antecedents in predicting healthcare knowledge-diffusing likelihood, followed by usefulness, source credibility and positivity. Further, the relationship between institution-based trust and healthcare knowledge-diffusing likelihood is partially mediated by source credibility.

Practical implications
Healthcare practitioners who seek to motivate individuals to disseminate healthcare knowledge need to phrase or frame healthcare knowledge in a way that draws greater interest, evokes stronger emotion, increases perceived usefulness or reflects positively on themselves. Healthcare organizations should also pay attention to strengthening users’ trust in the platform and source-related information that can indicate source authority.

Originality/value
This study is one of the first to investigate the dissemination of healthcare knowledge in the context of social media (WeChat in particular). Compared with other types of information, healthcare knowledge is more scientific and professional to the extent that most laypersons do not have relevant expertise to directly evaluate whether the content is credible and of high quality. Rather, their sharing likelihood is dependent more on other factors than perceived information quality and credibility; those factors include platform-related factors that may play an important role but has been overlooked in prior literature on interpersonal communication. By combining CET with interpersonal communication-related research and including institution-based trust as an important determinant of healthcare knowledge dissemination, this study provides a comprehensive analysis of healthcare knowledge diffusion process.
Exposure of chlorpyrifos in toddlers living in an agricultural area in Sakon Nakhon province, North-East Thailand
Authors: Siriwat Satinee , Rohitrattana Juthasiri , Nganchamung Thitirat , Ong-artborirak Parichat , Robson Mark , Siriwong Wattasit 
Published: February 2019

Purpose
Children living in agricultural areas are exposed to pesticides in their living areas and through activities of daily living. These exposures may lead to adverse health effects. The purpose of this paper is to investigate household environmental and behavioural factors associated with chlorpyrifos exposure and resultant adverse health effects in children living in an agricultural community.

Design/methodology/approach
A cross-sectional study was conducted including 65 toddlers (age of 12–36 months) and their parents were face-to-face interviewed from January to February 2016. Toddler’s hands and feet, toys and floors were wiped for chlorpyrifos residue analysis. The wipes were extracted and analysed by gas chromatography with a flame photometric detector, and blood cholinesterase activity was measured by the EQM Test-mate (model 400).

Findings
The average age (± standard deviation) of children was 19.9 (±5.9) months. Chlorpyrifos detections were 61.5 per cent (hands), 57.1 per cent (toys), 53.8 per cent (floors) and 30.8 per cent (feet). The highest chlorpyrifos residue concentration was detected on toy surface (3.022 µg/m2). Chlorpyrifos residues on hands and feet were positively correlated with concentrations on floors and toys (Spearman’s ρ, p<0.01). Multiple linear regression analysis revealed that more frequent hand washing (β=−0.236, p=0.067) and showering (β=−0.240, p=0.056) was negatively associated with chlorpyrifos residue on children’s body. House cleaning frequency was significantly associated with an increase in haemoglobin-adjusted erythrocyte cholinesterase (β=0.251, p<0.05).

Originality/value
Chlorpyrifos exposures found in the children household area through their activities and behaviours can cause several adverse health effects. The circumstances associated with chlorpyrifos exposure should be mitigated and reduced to improve the household environment of children living agricultural areas.
The impact of temporary staff on permanent staff in accident and emergency departments
Authors: Bajorek Zofia , Guest David 
Published: February 2019

PurposeThe purpose of this paper is to address a gap in the recent literature on employment of temporary workers by exploring the impact of temporary workers on the perceptions, attitudes and behaviour of permanent staff with particular reference to their implications for patient safety and service quality in hospital accident and emergency departments. The analysis is set in the context of the job demands-resources theory.Design/methodology/approachThe research was undertaken using a case study approach with semi-structured interviews in two London hospitals. Participants included staff from the HR director level, clinical managers and permanent staff who all had an influence in the hiring and management of temporary staff in some way. Transcripts were analysed thematically using an adopted framework approach.FindingsThe results indicate that the effect of temporary staff on permanent staff depended on the quality of the “resource”. There was a “hierarchy of preference” for temporary staff based on their familiarity with the context. Those unfamiliar with the department served as a distraction to permanent staff due to the need to “manage” them in various ways. While this was rarely perceived to affect patient safety, it could have an impact on service quality by causing delays and interruptions. In line with previous research, the use of temporary staff also affected perceptions of fairness and the commitment of some permanent staff.Practical implicationsA model developing an approach for improved practice when managing temporary staff was developed to minimise the risks to patient safety and service quality, and improve permanent staff morale.Social implicationsThe review highlights the difficulties that a limited amount of temporary staff integration can have on permanent staff and patient care, indicating that consideration must be placed on how temporary staff are inducted and clarifying expectations of roles for both temporary and permanent staff.Originality/valueThis paper studies the under-researched impact of temporary staff, and, distinctively, staff employed on a single shift, on the behaviour and attitudes of permanent staff. It highlights the need to consider carefully the qualitative nature of “resources” in the job demands-resources theory.
Sources of efficiency changes at Asian container ports
Authors: Yang Xiyi , Yip Tsz Leung 
Published: February 2019

Purpose
This study aims to investigate the efficiency changes of 23 major Asian container ports for the period from 2000 to 2007. In addition to assess the general trend, it also attempts to decompose the overall efficiency change into technological efficiency change, technical efficiency change and scale efficiency change to help port authorities to devise operational strategies.

Design/methodology/approach
The Malmquist index method is used, which is derived from data envelopment analysis. In this model, technological improvement comes from using state-of-the-art technologies, technical improvement is from rationalizing of port inputs and scale efficiency is from adjustment of port operational scales.

Findings
On average, the investigated ports have improved their efficiencies by 14.3 per cent. Such efficiency gains can be attributed to a 41 per cent increase in pure technical efficiency, a 47.5 per cent increase in scale efficiency and a 30.5 per cent decrease in technological efficiency. The scale efficiency contributes the most to the overall efficiency improvement, while technical and technological effects seem to have less impact. The fact that technological efficiency has little variance seems to suggest that this source of efficiency gain may not bring substantial competitive advantage.

Research limitations/implications
The sample period is 2000-2007, so the impact from the Asian financial crisis or the economic downturn was not covered. Also, the port throughputs data do not separate shipment and transhipment.

Originality/value
This study provides valuable suggestions to improve efficiency for container ports along the “Maritime Silk Road.”
The affective quality of sound: repositioning the listener
Authors: Macdonald Fiona Josephine 
Published: February 2019

Purpose
The purpose of this paper is to investigate the possibilities of performative research practices in the dissemination of social science research. The paper introduces the benefits of these practices and demonstrates the relational benefits of sound. The paper explores the possibility that sound may be used to reposition the listener to a new way of hearing.

Design/methodology/approach
This research emerged from a larger research project investigating the silent racism that was evident in an inclusive education program. A constructivist narrative approach was adopted to investigate the benefits of sharing the sensorial qualities of participant responses as an aural excerpt. The aim here is to reposition the listener from their own cultural value systems to being open to new understandings.

Findings
The paper highlights the relationship between the storyteller and the listener. Sharing a young man’s personal experience of racism enabled the visceral and affective quality of his deeply personal experience to be conveyed to the listener.

Research limitations/implications
This paper reports on the experiences of one participant. It is not designed to represent the experiences of all young people with African heritage, but rather to present the possibilities of using sound in the dissemination of research findings.

Originality/value
The methodological approach of this paper offers a unique and valuable contribution to the growing interest in new avenues to disseminate research findings, particularly those that convey the deeply personal experience of participants.
Getting up close and personal
Authors: Northcote Jeremy , Phillips Tarryn 
Published: February 2019

PurposeThe purpose of this paper is to evaluate the advantages and disadvantages of peer researchers in participant observation.Design/methodology/approachThe research involved interviews with 11 fieldworkers aged 18–25 years.FindingsThe method improves access to settings and provides useful context information on participants.Research limitations/implicationsIt is a useful method in situations where normal access to participants and settings is problematic.Originality/valueThe paper is the first ever evaluation of supervised peer research (as opposed to peer-led research).
Legitimacy-seeking strategies in the gambling industry: the case of responsible gambling
Authors: Leung Tiffany Cheng Han 
Published: February 2019

PurposeThe purpose of this paper is to examine how responsible gambling policies are communicated and presented as a legitimation strategy to different stakeholders.Design/methodology/approachThis study is based primarily on 49 semi-structured interviews with internal and external stakeholders of Macao’s gambling industry in 2011. This study draws on Reast et al.’s (2012) legitimacy-seeking strategy framework.FindingsThe findings indicate that these organisations use construing and earning legitimacy strategies to ensure passive support and acquiescence from certain stakeholder groups, and they deploy bargaining and capturing legitimacy strategies to generate active support for this morally contested industry. As a means of attaining long-standing legitimacy in the industry, gambling operators engage symbolically rather than substantively in responsible gambling to minimise the legitimacy gap.Research limitations/implicationsThe findings of the study pertain to a unique setting and might not be suitable for generalisation.Practical implicationsIn the absence of stringent legal mechanisms and strong external stakeholder pressure, the 12th Five-Year Plan of the People’s Republic of China aims to transform Macao into a “World Centre of Tourism and Leisure”, and gambling companies may soon face much stronger pressures from the Chinese Government and the Macao Government.Social/implicationsVoluntary responsible gambling initiatives are liable to be used only in symbolic fashion, without offering genuine engagement or full commitment to the most vulnerable stakeholder group.Originality/valueThis study contributes to the literature on social and accounting literature by providing an in-depth case study of how organisations in the gambling industry use different communication strategies to shape and respond to controversial issues.
Sustainability as source of competitive advantages in mature sectors
Authors: Settembre Blundo Davide , García-Muiña Fernando Enrique , Pini Martina , Volpi Lucrezia , Siligardi Cristina , Ferrari Anna Maria 
Published: February 2019

PurposeThe purpose of this paper is to explore how sustainability can become a source of competitive advantage for mature manufacturing sectors where technologies are standardized, and innovation is mainly generated across the value chain and not by individual companies.Design/methodology/approachFrom the methodological point of view, this research estimates the sustainability status of ceramic production in the Sassuolo district (Italy), using the Life Cycle Sustainability Assessment (LCSA) model, and changing the observation point for the analysis, from the enterprise (micro level) to the entire sector (meso level).FindingsThis paper provides an analysis of the environmental, economic and social impacts of the four main types of ceramic tiles manufactured in Italy, both in aggregate terms for the entire sector and per square meter of product.Practical implicationsThe methodological approach used in this research is easy to replicate both for companies when designing their sustainability strategies and for public decision makers when assessing the sustainability performance of a sector or supply chain.Social implicationsFor the first time, a socio-economic impact assessment is proposed for the ceramic sector, conducted in parallel with the environmental impact assessment through stakeholder mapping and prioritization.Originality/valueThis paper conceptualizes the theme of relations and interdependencies between ceramic producers organized in industrial districts and the territories in which they operate in order to determine empirically the sustainability performance of Italian ceramic sector, using the LCSA model with a territorial extension that presupposes an innovative contribution to current literature and practice.
Resveratrol protects against hepatic insulin resistance in a rat's model of non‐alcoholic fatty liver disease by down‐regulation of GPAT‐1 and DGAT2 expression and inhibition of PKC membranous translocation
Authors: Badi Rehab M. , Mostafa Dalia G. , Khaleel Eman F. , Satti Huda H. 
Published: February 2019

SummaryNon‐alcoholic fatty liver disease (NAFLD) is associated with hepatic insulin resistance (IR). Resveratrol (RES) a potent hypolipidemic dietary polyphenol has been identified for its ability to prevent hepatic steatosis and hepatic IR in high‐fat diet (HFD)‐fed murine models of NAFLD. In the present study, we have carried an in vivo animal experiment to identify a novel mechanism for RES protective action. Sub‐chronic (45 days) RES pretreatment in 3 days HFD‐fed adult Wistar rats prevented early hepatic IR through inhibiting PKC/JNK activation; decreasing p‐IRS (Ser307) and increasing p‐IRS(Tyr612), p‐Akt(Ser473) and p‐GSK3(Ser9). These effects of RES were associated with reduced expression of acyl‐CoA:glycerol‐sn‐3‐phosphate acyltransferase (GPAT‐1) and diacylglycerol:acyl‐CoA acyltransferase (DGAT2), two critical enzymes in the glycerol‐3‐phosphate pathway for de novo triglycerides synthesis. These data indicate that RES protects against NAFLD, initially, by inhibiting the early development of hepatic IR.
After the RESTART trial: six guidelines for clinical trials of early stuttering intervention
Authors: Onslow Mark , Lowe Robyn 
Published: February 2019

AbstractBackgroundThe Rotterdam Evaluation Study of Stuttering Therapy randomized trial (RESTART) was seminal, comparing the Lidcombe Program with RESTART Demands and Capacities Model‐based treatment (RESTART‐DCM) for pre‐school age children who stutter.AimsTo critique the methods of the RESTART trial to develop guidelines for its systematic replication and extension. Beyond that, to contribute to the refinement of existing methodological guidelines for early stuttering intervention.MethodThe discussion is organized around methodological issues of primary outcomes, treatment completion, clinician allegiance, treatment fidelity, age exclusions and no‐treatment control reasoning.Main ContributionWe recommend six methodological guidelines to guide future clinical trials comparing the Lidcombe Program with RESTART‐DCM, which can be applied to clinical trials of other early stuttering intervention methods: (1) incorporate a continuous measure of primary outcome; (2) ensure that all children in clinical trials have completed treatment; (3) eliminate potential bias due to clinician allegiance; (4) establish treatment fidelity within and beyond the clinic; (5) include children younger than 3 years in clinical trials; and (6) establish an estimate of treatment effect size at some stage of treatment development.ConclusionIn addition to guiding future clinical research comparing RESTART‐DCM and Lidcombe Program treatment, these recommendations may extend to influence positively other treatment developments for early stuttering.
Coping with low water activities and osmotic stress in Acinetobacter baumannii: significance, current status and perspectives
Authors: Zeidler Sabine , Müller Volker 
Published: February 2019

SummaryMultidrug resistant (MDR) pathogens are one of the most pressing challenges of contemporary health care. Acinetobacter baumannii takes a predominant position, emphasized in 2017 by the World Health Organization. The increasing emergence of MDR strains strengthens the demand for new antimicrobials. Possible targets for such compounds might be proteins involved in resistance against low water activity environments, since A. baumannii is known for its pronounced resistance against desiccation stress. Despite the importance of desiccation resistance for persistence of this pathogen in hospitals, comparable studies and precise data on this topic are rare and the mechanisms involved are largely unknown. This review aims to give an overview of the studies performed so far and the current knowledge on genes and proteins important for desiccation survival. ‘Osmotic stress’ is not identical to ‘desiccation stress’, but the two share the response of bacteria to low water activities. Osmotic stress resistance is in general studied much better, and in recent years it turned out that accumulation of compatible solutes in A. baumannii comprises some special features such as the bifunctional enzyme MtlD synthesizing the unusual solute mannitol. Furthermore, the regulatory pathways, as understood today, will be discussed.