bluuebunny/crossref_metadata_2025_split huggingface dataset:
The novel coronary artery disease risk gene JCAD/KIAA1462 promotes endothelial dysfunction and atherosclerosis
Authors: Xu Suowen , Xu Yanni , Liu Peng , Zhang Shuya , Liu Huan , Slavin Spencer , Kumar Sandeep , Koroleva Marina , Luo Jinque , Wu Xiaoqian , Rahman Arshad , Pelisek Jaroslav , Jo Hanjoong , Si Shuyi , Miller Clint L , Jin Zheng Gen 
Published: May 2019

Abstract

Aims
Recent genome-wide association studies (GWAS) have identified that the JCAD locus is associated with risk of coronary artery disease (CAD) and myocardial infarction (MI). However, the mechanisms whereby candidate gene JCAD confers disease risk remain unclear. We addressed whether and how JCAD affects the development of atherosclerosis, the common cause of CAD.

Methods and results
By mining data in the Genotype-Tissue Expression (GTEx) database, we found that CAD-associated risk variants at the JCAD locus are linked to increased JCAD gene expression in human arteries, implicating JCAD as a candidate causal CAD gene. We therefore generated global and endothelial cell (EC) specific-JCAD knockout mice, and observed that JCAD deficiency attenuated high fat diet-induced atherosclerosis in ApoE-deficient mice. JCAD-deficiency in mice also improved endothelium-dependent relaxation. Genome-wide transcriptional profiling of JCAD-depleted human coronary artery ECs showed that JCAD depletion inhibited the activation of YAP/TAZ pathway, and the expression of downstream pro-atherogenic genes, including CTGF and Cyr61. As a result, JCAD-deficient ECs attracted fewer monocytes in response to lipopolysaccharide (LPS) stimulation. Moreover, JCAD expression in ECs was decreased under unidirectional laminar flow in vitro and in vivo. Proteomics studies suggest that JCAD regulates YAP/TAZ activation by interacting with actin-binding protein TRIOBP, thereby stabilizing stress fiber formation. Finally, we observed that endothelial JCAD expression was increased in mouse and human atherosclerotic plaques.

Conclusion
The present study demonstrates that the GWAS-identified CAD risk gene JCAD promotes endothelial dysfunction and atherosclerosis, thus highlighting the possibility of new therapeutic strategies for CAD by targeting JCAD.
Satisfaction with mental health treatment among patients with a non-Western migrant background: a survey in a Danish specialized outpatient mental health clinic
Authors: Lindberg Laura Glahder , Mundy Sara Skriver , Kristiansen Maria , Johansen Katrine Schepelern , Carlsson Jessica 
Published: May 2019

Abstract

Background
Global migration increases ethnic and cultural diversity and demands mental health services to adapt to provide all patients with equal access to good quality care. Patient satisfaction surveys can inform this service delivery, thus we explored patient satisfaction among non-Western migrants receiving treatment in a Danish specialized outpatient mental health clinic [Competence Centre for Transcultural Psychiatry (CTP)].

Methods
We used multivariate logistic regression models to estimate associations between ‘Overall treatment satisfaction’ and treatment-related items plus potential confounders from a cross-sectional patient satisfaction survey (n = 686). The satisfaction questionnaire was a self-report measurement tool developed locally at CTP. Participants were non-Western migrants above 18 years with Post-Traumatic Stress Disorder (PTSD) or depression diagnoses according to ICD-10.

Results
Most participants (n = 497; 82.6%) reported overall satisfaction with their mental health treatment, but less than half (n = 311; 48.8%) reported an improvement in health and situation after end of treatment. Participants who experienced a subjective improvement in their health and situation had significantly higher odds of being satisfied with their mental health treatment [odds ratio (OR) = 8.5, 95% confidence interval (CI): 4.0–18.1]. Perceptions of influence on the treatment course (OR = 4.7, 95% CI: 2.4–9.2), and of understanding and respect for one’s cultural background (OR = 3.4, 95% CI: 1.5–7.6) were significantly associated with treatment satisfaction. Age and sex were insignificant in the final regression model.

Conclusions
Implications for practice based on our findings are to enhance person-centred care and shared decision-making with all patients regardless of cultural background and to prioritize pre- and postgraduate training in cultural competences and cultural humility for healthcare providers.
A Pas De Deux for a Cathedral and a Hunchback: Roland Petit’s Ballet Notre-Dame De Paris
Authors: Bührle Iris Julia 
Published: May 2019

Abstract
In 1965, the French choreographer Roland Petit set himself the task of making a ballet out of Victor Hugo’s voluminous novel Notre-Dame de Paris. The result departed radically from earlier attempts to transpose the work into movement by choreographers who included Jules Perrot and Marius Petipa. Although the love plot (which revolves around a dancer), the wide range of emotions depicted, the stark contrasts, and alternation between crowd scenes and more intimate scenes favour its transposition into a ballet, the novel also presents numerous difficulties, such as the importance of politics, philosophy and architecture. Quasimodo, Frollo and the cathedral seem rather unsuitable protagonists for a ballet. This paper argues that Petit’s adaptation of Notre-Dame de Paris engaged with the literary source on a much deeper level than its predecessors. Petit’s innovative ballet does not merely illustrate the novel: it reveals underlying elements in the source and sheds new light on it.
Black and Chestnut Oak Seedling Response to Glaciated Soil: Implications for Northward Expansion in Response to Climate Warming
Authors: Pile Lauren S , Rebbeck Joanne , Brose Patrick H , Long Robert P 
Published: May 2019

Abstract
Species ranges are expected to move northward with climate warming. However, species will likely encounter soil conditions that differ from their historic ranges. Black (Quercus velutina) and chestnut (Q. montana) oaks are expected to do well in climates that are hotter and drier, but it is unknown as to how they respond to soils of different geological histories. At a glaciated and nonglaciated site in northwestern Pennsylvania, we compared the growth, biomass allocation, and mycorrhizal association for black and chestnut oak seedlings at two different levels of light. On nutrient-poor, nonglaciated soils at high light levels, both species had greater growth and biomass accumulation than on nutrient-rich, glaciated soils. However, at low light levels, these responses were greatest on glaciated soils, especially for chestnut oak. Although mycorrhizal association was beneficial on nonglaciated soil at high light levels, low light levels were associated with reduced growth when compared to glaciated soils. In low light and nutrient-poor soils, mycorrhizal association may result in a carbon sink with lower net photosynthesis, as carbon for plant growth is, instead, allocated to the mycorrhizae symbiont. Variable species response to soil types indicates reduced performance for black and chestnut oak seedlings on high-quality glaciated soils especially in high light environments.
Cellular stressors may alter islet hormone cell proportions by moderation of alternative splicing patterns
Authors: Jeffery Nicola , Richardson Sarah , Chambers David , Morgan Noel G , Harries Lorna W 
Published: May 2019

Abstract
Changes to islet cell identity in response to type 2 diabetes (T2D) have been reported in rodent models, but are less well characterized in humans. We assessed the effects of aspects of the diabetic microenvironment on hormone staining, total gene expression, splicing regulation and the alternative splicing patterns of key genes in EndoC-βH1 human beta cells. Genes encoding islet hormones [somatostatin (SST), insulin (INS), Glucagon (GCG)], differentiation markers [Forkhead box O1 (FOXO1), Paired box 6, SRY box 9, NK6 Homeobox 1, NK6 Homeobox 2] and cell stress markers (DNA damage inducible transcript 3, FOXO1) were dysregulated in stressed EndoC-βH1 cells, as were some serine arginine rich splicing factor splicing activator and heterogeneous ribonucleoprotein particle inhibitor genes. Whole transcriptome analysis of primary T2D islets and matched controls demonstrated dysregulated splicing for ~25% of splicing events, of which genes themselves involved in messenger ribonucleic acid processing and regulation of gene expression comprised the largest group. Approximately 5% of EndoC-βH1 cells exposed to these factors gained SST positivity in vitro. An increased area of SST staining was also observed ex vivo in pancreas sections recovered at autopsy from donors with type 1 diabetes (T1D) or T2D (9.3% for T1D and 3% for T2D, respectively compared with 1% in controls). Removal of the stressful stimulus or treatment with the AKT Serine/Threonine kinase inhibitor SH-6 restored splicing factor expression and reversed both hormone staining effects and patterns of gene expression. This suggests that reversible changes in hormone expression may occur during exposure to diabetomimetic cellular stressors, which may be mediated by changes in splicing regulation.
A cross continental scale comparison of Australian offshore recreational fisheries research and its applications to Marine Park and fisheries management
Authors: Lynch T P , Smallwood C B , Ochwada-Doyle F A , Lyle J , Williams J , Ryan K L , Devine C , Gibson B , Jordan A 
Published: May 2019

Abstract
Recreational fishing is popular in Australia and is managed by individual states in consultation with the Commonwealth for those fisheries that they regulate and also for Australian Marine Parks (AMPs). Fishers regularly access both state and offshore Commonwealth waters but this offshore component of the recreational fishery is poorly understood. Our study tested the functionality of existing state-based surveys in Western Australia (WA) and New South Wales (NSW) to better inform Commonwealth fisheries and AMP managers about recreational fishing in their jurisdictions. Catch estimates for nine species of interest to the Commonwealth were developed and two case study AMPs [Ningaloo (WA) and The Hunter (NSW)] were also chosen to test the ability of the state survey data to be disaggregated to the park scale. As each state’s fishery survey designs were contextual to their own management needs, the application of the data to Commonwealth jurisdictions were limited by their statistical power, however aspects of each states surveys still provided useful information. Continued evolution of state-wide survey methods, including collection of precise spatial data, and regional over-sampling would be beneficial, particularly where there are multiple stakeholder and jurisdictional interests. National coordination, to temporally align state surveys, would also add value to the existing approaches.
A Randomized, Double-Blinded, Placebo-Controlled, Phase 1 Study of a Replication-Defective Herpes Simplex Virus (HSV) Type 2 Vaccine, HSV529, in Adults With or Without HSV Infection
Authors: Dropulic Lesia K , Oestreich Makinna C , Pietz Harlan L , Laing Kerry J , Hunsberger Sally , Lumbard Keith , Garabedian Doreen , Turk Siu Ping , Chen Aiying , Hornung Ronald L , Seshadri Chetan , Smith Malisa T , Hosken Nancy A , Phogat Sanjay , Chang Lee-Jah , Koelle David M , Wang Kening , Cohen Jeffrey I 
Published: May 2019

Abstract

Background
Herpes simplex virus 2 (HSV2) causes genital herpes in >400 million persons worldwide.

Methods
We conducted a randomized, double-blinded, placebo-controlled trial of a replication-defective HSV2 vaccine, HSV529. Twenty adults were enrolled in each of 3 serogroups of individuals: those negative for both HSV1 and HSV2 (HSV1−/HSV2−), those positive or negative for HSV1 and positive for HSV2 (HSV1±/HSV2+), and those positive for HSV1 and negative for HSV2 (HSV1+/HSV2−). Sixty participants received vaccine or placebo at 0, 1, and 6 months. The primary end point was the frequency of solicited local and systemic reactions to vaccination.

Results
Eighty-nine percent of vaccinees experienced mild-to-moderate solicited injection site reactions, compared with 47% of placebo recipients (95% confidence interval [CI], 12.9%–67.6%; P = .006). Sixty-four percent of vaccinees experienced systemic reactions, compared with 53% of placebo recipients (95% CI, −17.9% to 40.2%; P = .44). Seventy-eight percent of HSV1−/HSV2− vaccine recipients had a ≥4-fold increase in neutralizing antibody titer after 3 doses of vaccine, whereas none of the participants in the other serogroups had such responses. HSV2-specific CD4+ T-cell responses were detected in 36%, 46%, and 27% of HSV1−/HSV2−, HSV1±/HSV2+, and HSV1+/HSV2− participants, respectively, 1 month after the third dose of vaccine, and CD8+ T-cell responses were detected in 14%, 8%, and 18% of participants, respectively.

Conclusions
HSV529 vaccine was safe and elicited neutralizing antibody and modest CD4+ T-cell responses in HSV-seronegative vaccinees.

Clinical Trials Registration
NCT01915212.
A new syntopic species of Stygobromus Cope, 1872 (Amphipoda: Crangonyctidae) from groundwaters in Georgia and Florida, USA, with notes on S. floridanus Holsinger & Sawicki, 2016
Authors: Cannizzaro Andrew G , Balding Donna , Stine Michael , Sawicki Thomas R 
Published: May 2019

AbstractStygobromus doughertyensis n. sp. is described from groundwater habitats in Jackson County, Florida and Dougherty County, Georgia, USA using both morphological and molecular methods. This species occurs syntopically with the morphologically similar S. floridanus, which is newly recorded in Jackson County. The occurrence of S. floridanus in Jackson County marks a range extension for this species, which was described from two caves in Washington County, Florida. The description of S. doughertyensis n. sp. brings the number of described species in the genus Stygobromus to 142 and marks the second species of Stygobromus to be described from the Floridan aquifer.
Genetic diversity among populations of the Western Atlantic mangrove crab Ucides cordatus (Linnaeus, 1763) (Decapoda: Brachyura: Ocypodidae): evidence for panmixia and useful data for future management and conservation
Authors: Buranelli Raquel C , Felder Darryl L , Mantelatto Fernando L 
Published: May 2019

AbstractThe mangrove crab Ucides cordatus (Linnaeus, 1763) is a keystone species in mangroves, and recent changes in population sizes have been attributed primarily to overharvest, selective capture, and loss of habitat due to destruction of mangroves. We analyzed genetic variability and phylogeographic pattern among populations of U. cordatus along the western Atlantic coast in order to document levels of genetic differentiation, aiming to provide data to be used in future management and conservation strategies. MtDNA cytochrome oxidase I (COI) was analyzed and compared among populations. We computed a haplotype network, haplotype and nucleotide diversities, index of fixation, Mantel test, analysis of molecular variance (AMOVA), neutrality tests, and pairwise mismatch distributions under the sudden expansion model of demographic expansion; Maximum likelihood phylogenetic reconstruction was also undertaken for the haplotypes found. We found no geographic structure and no significant indication for restricted gene flow between populations from sites along a distance of 12,000 km, indicating that sampled individuals currently represent a single panmictic population. Demographic results revealed demographic expansion along the evolutionary history of U. cordatus, probably related to climatic biogeographic events. Although there is no current evidence of any substantial genetic break or of demographic reduction between the western Atlantic populations that we investigated, the genetic signal of regional population depletion warrants future monitoring in the course of resource management.
Evaluation of different kinds of disturbance on the efficiency of sampling methods employed for an endangered freshwater crayfish, Austropotamobius pallipes Lereboullet, 1858 (Decapoda: Astacidea: Astacidae), in the northern Iberian Peninsula
Authors: Olarte Nuria , García-Arberas Loreto , Antón Alvaro 
Published: May 2019

AbstractWe discuss the suitability of several sampling methods in terms of effort, efficiency, accuracy, obtained data, and the degree of disturbance in the endangered crayfish Austropotamobius pallipes (Lereboullet, 1858) and its habitat. Not all sampling strategies can be undertaken in headwater streams, and it is difficult to reach and work in remote areas. We compared three different sampling methods for five years at five sites in two small headwater streams in the Basque Country (Euskadi), northern Iberian Peninsula. We used night viewing from the river bank, hand searching during one removal pass, and the removal method by hand searching (abundance estimates) to determine their accuracy, efficiency, effort, and the disruption of crayfish and their habitat. Comparison of data between both relative abundance estimates, evaluated as captures per unit effort, were not significantly different between the estimates but they both differed significantly from the abundance estimates. Night hand searching or night viewing from the river bank required a longer surveying time and even some risk due to night work. Daylight manual searching could be consequently suitable if management of the species is supported by presence/absence data. The removal method is otherwise recommended when population estimates are required, even if it implies greater disturbance and effort from surveyors.
Annual and lunar breeding rhythmicity in females of the terrestrial hermit crab Coenobita rugosus H. Milne Edwards, 1837 (Decapoda: Anomura: Coenobitidae) in Okinawa, Japan
Authors: Osaka Naoya , Takemura Akihiro 
Published: May 2019

Abstract
We investigated the reproductive characteristics of a terrestrial hermit crab (Coenobita rugosus H. Milne Edwards, 1837) inhabiting Okinawa-Jima. Monthly changes in the gonadosomatic index and ovarian histology revealed that this species had fully developed oocytes from May to October. Data showing that females with (ovigerous females) and without (non-ovigerous females) embryos on their pleopods appeared simultaneously on the shore from June to September 2014 demonstrates that this species undergoes larval release several times for four months. When non-ovigerous females were reared under a combination of two day lengths and two temperatures, oocyte development was induced in the high-temperature group, suggesting that temperature is a proximate factor in ovarian development. The weekly collection of females during the spawning season revealed that the proportion of fully developed oocytes in an ovary increased toward the time of the new moon and decreased around the first-quarter moon, suggesting that this species is a lunar-spawner with a new-moon preference. Some females releasing larvae around the new or full moon were recaptured at approximately 30 d intervals, suggesting at least two major groups with lunar-synchronized larval release. The ovarian development and larval release of C. rugosus are likely entrained to external factors, which change periodically in their habitats.
A first fecundity study of the female snow crab Chionoecetes opilio Fabricius, 1788 (Decapoda: Brachyura: Oregoniidae) of the newly established population in the Barents Sea
Authors: Danielsen Hanna E H , Hjelset Ann M , Bluhm Bodil A , Hvingel Carsten , Agnalt Ann-Lisbeth 
Published: May 2019

Abstract
The snow crab Chionoecetes opilioFabricius, 1788 is a newly established species in the Barents Sea. Since the first individuals were recorded in 1996, the population has increased and a fishery was initiated in 2012. Mature female snow crab caught in the central Barents Sea were investigated with regards to fecundity. Ovigerous females (N = 185) ranged from 48 to 100 mm carapace width (CW). Fecundity was 15,000–184,000 eggs per female with 100% clutch fullness, and was positively related to body size (fecundity = 0.233 * CW2.944) consistent with relationships described for other snow crab populations. Eight females had less than 100% clutch fullness, two of which had only 10% clutch fullness along with an old shell, indicating senescence. Dry egg weight was on average 0.065 mg and was hardly affected by CW. Females across all sizes invest similarly in individual eggs, and potential size-dependent differences in fitness are more related to the number of eggs produced than to the investment into individual eggs. We conclude that the fecundity-at-size of females is overall comparable to that of other populations, although the presence of large females results in high maximum individual fecundity estimates.
A Sequential Mixed-Methods Study of Pre-Professionals’ Understanding of the Undergraduate Music Therapy Internship
Authors: Clements-Cortes Amy 
Published: May 2019

AbstractDespite the importance of the clinical music therapy internship, little research has been conducted to understand the perspectives, perceived musical, clinical, and personal skills, concerns, challenges, and anxieties of pre-professionals prior to and upon completion of the internship. This sequential mixed-methods study aimed to assess the perspectives and experiences of undergraduate students in the United States at two stages in the internship in music therapy practice. In total, 177 pre-professionals from the United States participated in this two-part study: (1) an online survey and (2) individual interviews with 25% (n = 44) of the participants. Survey results indicate statistically significant increases in clinical, musical, and personal skill development from pre- to post-internship. Six broad categories emerged from the interviews: confidence, anxiety, role clarity, professional suitability, loneliness, and boundaries/ethics. The results are encouraging, showing that the internship is a valued clinical experience that fosters improvement in the clinical, musical, and personal skills needed to work as a music therapist. This paper concludes with recommendations and suggestions for educators and supervisors on preparing and supporting pre-professionals in their skill development prior to and during internship.