bluuebunny/crossref_metadata_2025_split huggingface dataset:
Studying the emergence of a new social representation: Changes in thinking about nanotechnologies in early 21st‐century Italy
Authors: Brondi Sonia , Neresini Federico 
Published: February 2018

AbstractWe investigated the emergence of a new social representation (SR) of a techno‐scientific innovation—nanotechnologies—among the Italian public. We reviewed how nanotechnologies entered parliamentary debates and the media agenda in the early third millennium. We conducted cross‐sectional surveys in 2006 (N = 246) and 2011 (N = 486) to examine the emerging SR of nanotechnologies. We sought to observe processes of anchoring and objectification ‘in action’, by analyzing roles of (i) social groups, and (ii) neighboring SRs of science and of technology, over time. Several changes from 2006 to 2011 were identified: From a ‘descriptive’ to an ‘evaluative’ approach; from a ‘neutral’ to a ‘controversial’ issue; from a ‘concrete’ to an ‘abstract’ object; and from a ‘technological’ to a ‘scientific’ phenomenon. We conclude that nanotechnologies finally became ‘relevant enough’ by 2011 to be considered a proper object of SR, and an emerging SR can be observed.
Assessment of the impact of different vegetation patterns on soil erosion processes on semiarid loess slopes
Authors: Feng Tianjiao , Wei Wei , Chen Liding , Rodrigo‐Comino Jesús , Die Chen , Feng Xinran , Ren Kemeng , Brevik Eric C. , Yu Yang 
Published: February 2018

AbstractSoil erosion hinders the recovery and development of ecosystems in semiarid regions. Rainstorms, coupled with the absence of vegetation and improper land management, are important causes of soil erosion in such areas. Greater effort should be made to quantify the initial erosion processes and try to find better solutions for soil and water conservation. In this research, 54 rainfall simulations were performed to assess the impacts of vegetation patterns on soil erosion in a semiarid area of the Loess Plateau, China. Three rainfall intensities (15 mm h‐1, 30 mm h‐1 and 60 mm h‐1) and six vegetation patterns (arbors‐shrubs‐grass ‐A‐S‐G‐, arbors‐grass‐shrubs ‐A‐G‐S‐, shrubs‐arbors‐grass ‐S‐A‐G‐, shrubs‐grass‐arbors ‐S‐G‐A‐, grass‐shrubs‐arbors ‐G‐S‐A‐ and grass‐arbors‐shrubs ‐G‐A‐S‐) were examined at different slope positions (summits, backslopes and footslopes) in the plots (33.3%, 33.3%, 33.3%), respectively. Results showed that the response of soil erosion to rainfall intensity differed under different vegetation patterns. On average, increasing rainfall intensity by 2 to 4 times induced increases of 3.1 to 12.5 times in total runoff and 6.9 to 46.4 times in total sediment yield, respectively. Moreover, if total biomass was held constant across the slope, the patterns of A‐G‐S and A‐S‐G (planting arbor at the summit position) had the highest runoff (18.34 L m‐2 h‐1) and soil losses (197.98 g m‐2 h‐1), while S‐A‐G had the lowest runoff (5.51 L m‐2 h‐1) and soil loss (21.77 g m‐2 h‐1). As indicated by redundancy analysis (RDA) and Pearson correlation results, a greater volume of vegetation located on the back‐ and footslopes acted as effective buffers to prevent runoff generation and sediment yield. Our findings indicated that adjusting vegetation position along slopes can be a crucial tool to control water erosion and benefit ecosystem restoration on the Loess Plateau and other similar regions of the world. Copyright © 2018 John Wiley & Sons, Ltd.
Benefit‐Cost Analysis and Emerging Technologies
Authors: Mannix Brian 
Published: February 2018

AbstractEmerging technologies are, by definition, full of surprises: developments that we cannot fully anticipate and that might have some bad outcomes as well as good ones. This presents a challenge for anyone trying to make forward‐looking policy decisions, including those who apply benefit‐cost analysis. BCA is now widely known and used, but it is also widely misunderstood—by many of its advocates as well as its detractors. In this essay, I will begin by examining some of the strengths and weaknesses of BCA as a normative science. Yes, “normative science” is an oxymoron, and the incongruity it speaks to is a source of much of the controversy; BCA is an imperfect answer, but often the best available answer, to the question of how a society should go about making collective but not unanimous choices.I also want to take a hard look at the question of what we are evaluating. BCA is designed to weigh government actions, to see whether they are in the public interest; it is not designed to evaluate private actions. But if the government action is to approve a private action that otherwise would be prohibited, then the BCA inevitably must evaluate the latter. From the perspective of a government regulator, it is very tempting to shift the burden of proof onto private innovators—to obligate them to seek permission to proceed, and to demonstrate that their proposed actions have acceptable risks and will produce a net social benefit. But such burden‐shifting is not merely an administrative convenience; it has serious economic consequences that need to be examined.
Lessons from Environmental Regulation
Authors: Sinden Amy 
Published: February 2018

AbstractMuch of the most substantive and in‐depth experience with formal cost‐benefit analysis in the public policy realm has occurred in the context of federal environmental regulation in the United States. This experience has many important lessons to teach in the realm of synthetic biology. Indeed, many of the dangers and pitfalls that arise when decision‐makers use formal CBA to evaluate environmental regulation seem likely to arise in the synthetic biology context as well, sometimes in particularly troubling forms. Unfortunately, while in many instances these concerns may well point toward a rejection of formal CBA for synthetic biology, the experience from environmental regulation turns out to be far less helpful in identifying alternative decision‐making tools. Because the decisions that arise in the synthetic biology context have a fundamentally different structure from decisions about environmental regulation, the most useful alternatives from that context do not map easily onto this new context.It may well be generally true that in the search for decision‐making tools, we should not be looking for a single silver bullet that will work in all public policy realms. Perhaps, instead, different kinds of decision‐making call for different tools. This may be true even within the realm of synthetic biology. I am not entirely sure what the “right” tool is for synthetic biology applications, or even whether a “right” tool exists. But at the end of this essay, I offer a few tentative thoughts about why scenario analysis—a strategic planning tool first developed in the context of military planning following World War II—might be one alternative worth considering.
Integrating Scenario Planning and Cost‐Benefit Methods
Authors: Aldrich Stephen C. 
Published: February 2018

AbstractBy their nature, the most vexing social problems reflect collisions between social and economic interests of parties with highly divergent views and perspectives on the cause and character of what is at issue and the consequences that flow from it. Conflicts around biotechnology applications are good examples of these problems. When considering the potential consequences of proposed biotechnology applications, an enormous range of perspectives arise reflecting the breadth of different and often competing interests with a stake in life's future.This essay starts from an assumption that the traditional tool of cost‐benefit analysis is not adequate for adjudicating competing claims around the introduction of new biotechnology applications. It tends to require implicit simplifying assumptions that reduce or mask true underlying levels of complexity and uncertainty, and the results it produces deliver a definitive and singular answer, as opposed to a multiplicity of outcomes. In this essay, I describe some of the key elements of formal scenario planning to show how CBA could be redeployed as a supporting tool within the broader decision support methodology of formal scenario planning.
Beyond Cost‐Benefit Analysis in the Governance of Synthetic Biology
Authors: Wallach Wendell , Saner Marc , Marchant Gary 
Published: February 2018

AbstractFor many innovations, oversight fits nicely within existing governance mechanisms; nevertheless, others pose unique public health, environmental, and ethical challenges. Synthetic artemisinin, for example, has many precursors in laboratory‐developed drugs that emulate natural forms of the same drug. The policy challenges posed by synthetic artemisinin do not differ significantly in kind from other laboratory‐formulated drugs. Synthetic biofuels and gene drives, however, fit less clearly into existing governance structures. How many of the new categories of products require new forms of regulatory oversight, or at least extensive forms of testing, remains unclear.Any effort to improve the governance of synthetic biology should start with a rich understanding of the different possible science‐policy interfaces that could help to inform governance. CBA falls into a subset of the overall range of possibilities, and which interface is appropriate may turn out to depend on context, on the demands of the decision at hand. In what follows, we lay out a typology of interfaces. After that, we turn to the question of how to draw upon the range of possible interfaces and effectively address the factual and moral complexities of emerging technologies. We propose a governance model built around structures that we call “governance coordinating committees.” GCCs are intended to be mechanisms for accommodating the complexities of innovations that have far‐ranging societal impacts. The production of biofuels, for example, could contaminate water supplies and have a destructive environmental impact if not managed correctly. The introduction of a gene drive could have economic and environmental impacts that are not restricted to one nation. Forging appropriate means for determining and evaluating those societal impacts, to the best of a corporation's, industry's, or government's ability, is central to responsible research and innovation. Public policy must be shaped in a manner that accommodates as many concerns as possible and minimizes risks.
CBA and Precaution: Policy‐Making about Emerging Technologies
Authors: Kaebnick Gregory E. , Gusmano Michael K. 
Published: February 2018

AbstractIn the technology assessment literature, the leading alternative to CBA‐like methods is usually held to be precaution, which is understood in various ways but is always about making decisions under conditions of uncertainty. Under such conditions, proponents of precaution commonly hold, a straightforward tallying of potential outcomes does not seem possible. Since CBA aims to tally up outcomes to determine which outcome would produce the greatest public benefit, precaution begins to look like, not just an alternative to CBA, but an incompatible alternative.Nonetheless, some of the better‐known formulations of a precautionary principle expressly call for combining precaution with assessment of costs and benefits. This essay examines the possible intersection of precaution and CBA. It argues that a moderate kind of CBA is a necessary part of a moderate kind of precaution. The existing proposals for integrating CBA and precaution start with an assumption that the integrative task consists in combining decision tools that generate (contrasting) substantive guidance. An alternative approach, explored here, starts with the idea that precaution is not a decision‐generating tool. Rather, it is a way of organizing the thinking that leads eventually to substantive conclusions. The appropriate policy response is reached not by applying a principle but by studying the situation—the proposed action and the problem it is meant to address—and developing recommendations tailored to it. What makes the thinking precautionary is that it emphasizes certain questions—about risk, uncertainty, and values—that CBA tends to suppress. So understood, precaution may well slow the science but is not intrinsically opposed to science or innovation. It can be understood, in fact, as continuous with the science because the contextual understanding of the science and the problems it is meant to address would emerge—in part—from a close engagement with the science.
Tunable poly(methacrylic acid‐co‐acrylamide) nanoparticles through inverse emulsion polymerization
Authors: Zhong Justin X. , Clegg John R. , Ander Eric W. , Peppas Nicholas A. 
Published: February 2018

AbstractEnvironmentally responsive biomaterials have played key roles in the design of biosensors and drug delivery vehicles. Their physical response to external stimuli, such as temperature or pH, can transduce a signal or trigger the release of a drug. In this work, we designed a robust, highly tunable, pH‐responsive nanoscale hydrogel system. We present the design and characterization of poly(methacrylic acid‐co‐acrylamide) hydrogel nanoparticles, crosslinked with methylenebisacrylamide, through inverse emulsion polymerization. The effects of polymerization parameters (i.e., identities and concentrations of monomer and surfactant) and polymer composition (i.e., weight fraction of ionic and crosslinking monomers) on the nanoparticles’ bulk and environmentally responsive properties were determined. We generated uniform, spherical nanoparticles which, through modulation of crosslinking, exhibit a volume swelling of 1.77–4.07, relative to the collapsed state in an acidic environment. We believe our system has potential as a base platform for the targeted, injectable delivery of hydrophilic therapeutics. With equal importance, however, we hope that our systematic analysis of the individual impacts of polymerization and purification conditions on nanoparticle composition, morphology, and performance can be used to expedite the development of alternate hydrophilic nanomaterials for a range of biomedical applications. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1677–1686, 2018.
Evaluation of a recombinant multiepitope antigen for diagnosis of hepatitis C virus: A lower cost alternative for antigen production
Authors: Thomasini Ronaldo Luis , Souza Hortencia Gisele Amaro , Bruna‐Romero Oscar , Totola Antonio Helvecio , Gonçales Neiva Sellan Lopes , Lima Cristiano Xavier , Teixeira Mauro Martins 
Published: February 2018

BackgroundThe most of the hepatitis C‐infected patients remain undiagnosed until they develop severe liver damage or submitted for serological screening.ObjectiveTo evaluate a recombinant multiepitope protein for detection of IgG anti‐hepatitis C virus. Method: A synthetic gene was cloned, expressed in Escherichia coli, and the recombinant protein was purified. Human serum panel consisted of 88 positives (20 HCV genotyped) and 376 negatives for hepatitis C, 6 positives for human acquired immunodeficiency virus, 6 syphilis positives, 6 hepatitis B positives were tested by IgG antihepatitis C virus using the protein by enzyme‐linked immunosorbent assay. In addition, 20 positive (all genotyped samples) and 20 negative samples were also tested by immunoblot and dot blot assays.ResultsPositive hepatitis C sera were strongly reactive against the protein by immunoblot assay. In the dot blot assay, positive sera were reactive until 1:1000 dilution and there were no false positive results in the hepatitis C negative sera. In the enzyme‐linked immunosorbent assay, positive and negative sera had significant discrimination. No cross‐reaction was observed in samples positive for syphilis; human acquired immunodeficiency virus and hepatitis B. All 20 genotyped samples were positive by the three methods.ConclusionThe multiepitope protein used here has a lower cost compared to production of each antigen separately and could be an alternative for the serological diagnosis of hepatitis C.
Preclinical evaluation of a semi‐automated and rapid commercial electrophoresis assay for von Willebrand factor multimers
Authors: Pikta Marika , Zemtsovskaja Galina , Bautista Hector , Nouadje Georges , Szanto Timea , Viigimaa Margus , Banys Valdas 
Published: February 2018

BackgroundThe von Willebrand factor (VWF) multimer test is required to correctly subtype qualitative type 2 von Willebrand disease (VWD). The current VWF multimer assays are difficult, nonstandardized, and time‐consuming. The purpose of this study was to evaluate the clinical utility of the commercial VWF multimer kit by Sebia (Lisses, France), an electrophoresis technique yielding same‐day results.MethodsTen healthy volunteer plasma samples, in‐house reference plasma (IRP) and commercial normal plasma (CNP) samples, 10 plasma samples from patients with a known VWD type, 1 hemophilia A plasma sample, and 7 external quality assurance (EQA) samples were analyzed using the commercial VWF multimer kit. Additional coagulation testing included measurements of VWF antigen (VWF:Ag), VWF activity (VWF:Ac), and FVIII activity (FVIII:C).ResultsThe CNP results revealed a relative loss of the highest molecular weight multimers; therefore, IRP was preferred as the reference sample. The interpretations of 10 patients with a known VWD type could be successfully reproduced and agreed with previous VWF multimer results. In all EQA surveys, the multimer results and final VWD diagnosis agreed with expert opinion.ConclusionsThe VWF multimer assay by Sebia is easy to perform and can be successfully implemented in any clinical laboratory for second‐stage evaluation of VWD. The resolution power of multimer distribution is adequate to correctly classify VWD types 1, 2A, 2B, and 3.
Three‐dimensional printing in congenital heart disease: A systematic review
Authors: Lau Ivan , Sun Zhonghua 
Published: February 2018

AbstractThree‐dimensional (3D) printing has shown great promise in medicine with increasing reports in congenital heart disease (CHD). This systematic review aims to analyse the main clinical applications and accuracy of 3D printing in CHD, as well as to provide an overview of the software tools, time and costs associated with the generation of 3D printed heart models. A search of different databases was conducted to identify studies investigating the application of 3D printing in CHD. Studies based on patient's medical imaging datasets were included for analysis, while reports on in vitro phantom or review articles were excluded from the analysis. A total of 28 studies met selection criteria for inclusion in the review. More than half of the studies were based on isolated case reports with inclusion of 1–12 cases (61%), while 10 studies (36%) focused on the survey of opinion on the usefulness of 3D printing by healthcare professionals, patients, parents of patients and medical students, and the remaining one involved a multicentre study about the clinical value of 3D printed models in surgical planning of CHD. The analysis shows that patient‐specific 3D printed models accurately replicate complex cardiac anatomy, improve understanding and knowledge about congenital heart diseases and demonstrate value in preoperative planning and simulation of cardiac or interventional procedures, assist surgical decision‐making and intra‐operative orientation, and improve patient‐doctor communication and medical education. The cost of 3D printing ranges from USD 55 to USD 810. This systematic review shows the usefulness of 3D printed models in congenital heart disease with applications ranging from accurate replication of complex cardiac anatomy and pathology to medical education, preoperative planning and simulation. The additional cost and time required to manufacture the 3D printed models represent the limitations which need to be addressed in future studies.
Inflammatory and degenerative phases resulting from anterior cruciate rupture in a non‐invasive murine model of post‐traumatic osteoarthritis
Authors: Gilbert Sophie J. , Bonnet Cleo S. , Stadnik Paulina , Duance Victor C. , Mason Deborah J. , Blain Emma J. 
Published: February 2018

ABSTRACTJoint injury is the predominant risk factor for post‐traumatic osteoarthritis development (PTOA). Several non‐invasive mouse models mimicking human PTOA investigate molecular mechanisms of disease development; none have characterized the inflammatory response to this acute traumatic injury. Our aim was to characterize the early inflammatory phase and later degenerative component in our in vivo non‐invasive murine model of PTOA induced by anterior cruciate ligament (ACL) rupture. Right knees of 12‐week‐old C57Bl6 mice were placed in flexion at a 30° offset position and subjected to a single compressive load (12N, 1.4 mm/s) to induce ACL rupture with no obvious damage to surrounding tissues. Tissue was harvested 4 h post‐injury and on days 3, 14, and 21; contralateral left knees served as controls. Histological, immunohistochemical, and gene analyzes were performed to evaluate inflammatory and degenerative changes. Immunohistochemistry revealed time‐dependent expression of mature (F4/80 positive) and inflammatory (CD11b positive) macrophage populations within the sub‐synovial infiltrate, developing osteophytes, and inflammation surrounding the ACL in response to injury. Up‐regulation of genes encoding acute pro‐inflammatory markers, inducible nitric oxide synthase, interleukin‐6 and interleukin‐17, and the matrix degrading enzymes, ADAMTS‐4 and MMP3 was detected in femoral cartilage, concomitant with extensive cartilage damage and bone remodelling over 21‐days post‐injury. Our non‐invasive model describes pathologically distinct phases of the disease, increasing our understanding of inflammatory episodes, the tissues/cells producing inflammatory mediators and the early molecular changes in the joint, thereby defining the early phenotype of PTOA. This knowledge will guide appropriate interventions to delay or arrest disease progression following joint injury. © 2018 The Authors. Journal of Orthopaedic Research® Published by Wiley Periodicals, Inc. on behalf of the Orthopaedic Research Society. J Orthop Res 36:2118–2127, 2018.
Fingerprinting sediment sources in a large agricultural catchment under no‐tillage in Southern Brazil (Conceição River)
Authors: Tiecher Tales , Minella Jean Paolo Gomes , Evrard Olivier , Caner Laurent , Merten Gustavo Henrique , Capoane Viviane , Didoné Elizeu Jonas , dos Santos Danilo Rheinheimer 
Published: February 2018

AbstractBrazil is a leader in the adoption of conservation agriculture practices and technologies. However, the impact of these practices on sediment sources at the catchment scale has not been quantified yet, particularly in grain growing regions, where a conservationist no‐tillage system is implemented to protect soils. To address this knowledge gap, a sediment fingerprinting study based on elemental geochemistry was carried out in a large agricultural catchment (804 km2) of Southern Brazil where no‐tillage practices dominate. A total of 156 soil samples were taken to characterize the three main potential sediment sources: cropland (n = 79), unpaved roads (n = 41), and channel banks (n = 36). Sediment sampling was performed using a time‐integrated sampler (n = 33) and by collecting fine‐bed material (n = 34) at five locations across the catchment. Sediment was also sampled during flood events at the catchment outlet (n = 20). Sediment source contributions were calculated using an optimal suite of geochemical properties and a mixing model. Results showed that although the catchment is not particularly sensitive to soil erosion (i.e., deep clayey soils with gentle slopes), the amount of sediment supplied by cropland to the river network remains very high (up to 1.63 Mg·ha−1·year−1). Sediment fingerprinting results showed that even when no‐tillage is implemented, cropland remains an important source of sediment, supplying up to 70% of the material transiting the Conceição River. Accordingly, the current conservation farming system in this catchment needs to be improved to further reduce soil erosion and sediment yield.