bluuebunny/crossref_metadata_2025_split huggingface dataset:
Application of bio‐based dimer acid diglycidyl ester in paper‐based copper clad laminate
Authors: Sun Yiang , Zong Chuanhui , Wang Fei , Zhang Na , Li Aixiang , Li Qiuhong , LYu Zijian 
Published: January 2019

ABSTRACTUsing dimer acid (DA) as raw material, DA diglycidyl ester (DADGE) was synthesized and used as reactive toughening agent to prepare paper‐based copper clad laminate (p‐CCL). The factors affecting the epoxy value of DADGE and the effect of the resin on the gelation time were studied. The effects of the epoxy value and the addition amount of DADGE on the solderleaching resistance, flammability, water absorption, bending strength, and impact strength of the p‐CCL were discussed. The results showed when the molar ratio of DA to ECH was 1:8 and the molar ratio of DA to sodium hydroxide was 1:1.6, the epoxy value of DADGE reached the maximum value of 0.23 mol/100 g. The DADGE can shorten the gelation time of the glue. The p‐CCL meets the performance of the IPC‐TM‐650 standard. And when the addition amount of DADGE is less than 12 wt %, the flammability of the p‐CCL reaches UL94V‐0 level. The p‐CCL prepared by adding 6 wt % of DADGE with 0.08 mol/100 g epoxy value has the best comprehensive performance, its toughness and rigid are comparable to those of p‐CCL with 12 wt % of commercially available high performance toughening agents and it has higher solderleaching resistance. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 136, 47508.
Synthesis, Structures, and Single‐Molecule Magnetic Properties of Three Dy2 Complexes
Authors: Ge Yu , Huang Yuan , Montenegro Jessenia Lisseth Becerra , Cui Yanfeng , Liu Wei , Li Yahong , Wang Bao‐Lin 
Published: January 2019

AbstractTo explore the influences of the subtle structural variations in the ligand backbones on the single‐molecule magnetic properties of dinuclear dysprosium(III) complexes, three ligands—H2L1 (H2L1=N1,N3‐bis(salicylaldehyde)diethylenetriamine), H2L2 (H2L2=N1,N3‐bis(3‐methoxysalicylidene)diethylenetriamine), and H2L3 (H2L3=N1,N3‐bis(5‐chlorosalicyladehyde)diethylenetriamine)—were synthesized and employed to prepare the expected dinuclear dysprosium(III) complexes. The three ligands differ in terms of the substituents at the benzene rings of the salicylaldehyde moieties. The reactions of Dy(NO3)3⋅6 H2O, pivalic acid, and the ligands H2L1, H2L2, and H2L3 generated complexes with the formulae [Dy2(L1)2(piv)2] (1), [Dy2(L2)2(piv)2] (2), and [Dy2(L3)2(piv)2]⋅ 2 MeCN (3), respectively. The purposeful attachment of the functional groups with varied sizes at the benzene rings of the salicylaldehyde backbones resulted in slight differences in the Dy‐O‐Dy bond angles and the Dy⋅⋅⋅Dy bond lengths in 1–3; consequently, the three complexes exhibited distinct magnetic properties. They all showed slow magnetization relaxation with energy barriers of 40.32 (1), 31.67 (2), and 33.53 K (3). Complete active space self‐consistent field (CASSCF) calculations were performed on complexes 1–3 to rationalize the slight discrepancy observed in the magnetic behavior. The calculated results satisfactorily explained the experimental outcomes.
Exploring the Promiscuous Enzymatic Activation of Unnatural Polyketide Extender Units in Vitro and in Vivo for Monensin Biosynthesis
Authors: Grote Marius , Schulz Frank 
Published: January 2019

AbstractThe incorporation of new‐to‐nature extender units into polyketide synthesis is an important source for diversity yet is restricted by limited availability of suitably activated building blocks in vivo. We here describe a straightforward workflow for the biogenic activation of commercially available new‐to‐nature extender units. Firstly, the substrate scope of a highly flexible malonyl co‐enzyme A synthetase from Streptomyces cinnamonensis was characterized. The results were matched by in vivo experiments in which the said extender units were accepted by both the polyketide synthase and the accessory enzymes of the monensin biosynthetic pathway. The experiments gave rise to a series of predictable monensin derivatives by the exploitation of the innate substrate promiscuity of an acyltransferase and downstream enzyme functions.
Glutamate as an Efficient Amine Donor for the Synthesis of Chiral β‐ and γ‐Amino Acids Using Transaminase
Authors: Kim Geon‐Hee , Jeon Hyunwoo , Khobragade Taresh P. , Patil Mahesh D. , Sung Sihyong , Yoon Sanghan , Won Yumi , Sarak Sharad , Yun Hyungdon 
Published: January 2019

AbstractA recyclable glutamate amine donor system employing transaminase (TA), glutamate dehydrogenase (GluDH) and mutant formate dehydrogenase (FDHm) was developed, wherein amine donor Glu was regenerated using GluDH and thereby circumvented the inhibition of TA by α‐ketoglutarate. Various enantiopure β‐, γ‐amino acids, and amines were successfully synthesized with high conversions and excellent enantiomeric excess using this system.
Prevention is better than cure—Environmental management measures in hospitals
Authors: Seifert Christin , Guenther Edeltraud 
Published: January 2019

AbstractHospitals are major actors in the health care sector, and their duty is the safe treatment of patients. Thus, hospitals make an important contribution to the achievement of sustainability goals. However, they also have negative environmental impacts. Hospitals have difficulties responding to increasing environmental demands. The aim of this paper is to systematically delineate measures for hospitals' environmental management.We research EMAS‐registered hospitals as pioneers in environmental management that have credibility through external validation. Based on 31 publicly available environmental statements, we analyze measures from the environmental programs. These measures are classified into general management approaches and measures that are related to input (energy, materials, and water) and output (waste, wastewater, and emissions).The paper makes a practical contribution by summarizing environmental measures to improve environmental performance. This provides a brief overview that can serve as a benchmark and best‐practice sharing for other hospitals and organizations interested in environmental protection.
Exploring the Capability of LC‐MS and GC‐MS Multi‐Class Methods to Discriminate Virgin Olive Oils from Different Geographical Indications and to Identify Potential Origin Markers
Authors: Olmo‐García Lucía , Wendt Karin , Kessler Nikolas , Bajoub Aadil , Fernández‐Gutiérrez Alberto , Baessmann Carsten , Carrasco‐Pancorbo Alegría 
Published: January 2019

Looking for a strategy to authenticate the declared origin of commercial extra virgin olive oils (EVOOs), 126 samples from six different Mediterranean geographical indications (GIs) are analyzed by means of two different platforms [LC‐ESI‐QTOF MS (in positive and negative polarity) and GC‐APCI‐QTOF MS (in positive mode)] combined to chemometrics. The sample treatment and chromatographic/detection conditions (in both platforms) are chosen to enable the comprehensive characterization of the complete minor fraction of the oils within a single run. Noticeable discrimination among the six evaluated GIs [Priego de Córdoba and Baena (Spain), Kalamata (Greece), Toscano (Italy), and Ouazzane and Meknès (Morocco)] is achieved building two‐class PLS‐DA models which consider the data coming from both platforms. The contribution of a few thousand molecular features to the statistical models is evaluated in depth and several compounds are pointed out as possible origin markers, describing characteristic compositional patterns for each GI in the evaluated crop year. The complementarity of the different analytical approaches is discussed and diverse strategies are used to identify the classifiers.Practical Applications: Protected Designation of Origin (PDO) and Protected Geographical Indication (PGI) labels are important tools to promote high quality EVOOs, assuring the connection to a particular territory and the characteristic combination of natural and human factors which make possible to obtain unique oils. In this context, it is imperative to furnish the control labs with innovative tools and methods which are able to provide extensive information about the EVOOs’ minor fraction (of unquestionable importance regarding its overall quality) in just one run and to give the chance to find and identify (and validate) origin markers. The utility of validated classifiers to authenticate the belonging (or not) of an EVOO to a particular GI is clear. The consumers’ confidence can be perceptibly undermined if the geographical name is used on products not having the expected qualities or if the production specifications are sometimes not followed by producers.The capability of LC‐MS and GC‐MS multi‐class methods to discriminate virgin olive oils from different geographical indications (crop season 2016‐2017) and to identify potential origin markers is demonstrated.
Minimally Invasive & Long‐lasting Neural Probes from a Materials Perspective
Authors: Veronica Asmita , Li Yue , Hsing I‐ming 
Published: January 2019

AbstractNeurological disorders in aging society have created the need to understand how the brain works. At present, there are limited engineered “non‐invasive” tools to study and characterize the brain activity. Advancement in neuroscience research is highly focused on the fabrication of implantable neural probes that can effectively integrate with the neural tissue. Challenges associated with the commercially available probes has driven the development of mechanically compliant and non‐invasive neural implants. Engineering the mechanical and electrical performance of these probes is necessary to obtain tremendous sensitivity and selectivity along with chronic functionality. Selection of proper materials and understanding their fundamentals plays an essential role in achieving the desired neural interface. In this review, we have highlighted the recent progress in the utilization of new materials to attain a neural probe with improved flexibility, biocompatibility and signal quality.
Resolution of a Mononitrophenol Isomers Mixture by Differential Alternative Pulses Voltammetry
Authors: Stoytcheva Margarita , Zlatev Roumen , Velkova Zdravka , Gochev Velizar , Ayala Alan , Montero Gisela , Valdez Benjamín 
Published: January 2019

AbstractThe high resolution second order voltammetric technique, Differential Alternative Pulse Voltammetry (DAPV), was applied for the simultaneous quantification of the mononitrophenol (NP) isomers. Complete resolution of the three isomers was achieved at concentration ratios as high as 1 : 5, employing the corresponding anodic and cathodic peaks appeared on the DAPV curve. The working glassy carbon electrode was modified by a nanocomposite of graphite nanopowder and Au nanoparticles to increase the sensitivity. The linear concentration range was found to be extended up to 125 μmol L−1 for both the m‐NP and p‐NP isomers, while the o‐NP isomer presented calibration plot of two linear sections: up to 100 μmol L−1 and up to 225 μmol L−1. The LOD was found to be as low as 1.5 μmol L−1, 2.5 μmol L−1, and 0.5 μmol L−1 for o‐NP, m‐NP, and p‐NP, respectively. The accuracy of the proposed method was evaluated by quantification of spiked tap waters samples. No interference was observed from a range of phenolic compounds such as phenol, 4‐aminophenol, 4‐chlorophenol, 2,4‐dinitrophenol, and resorcinol.
A smart and portable micropump for stable liquid delivery
Authors: Zhang Xinjie , Xia Kang , Ji Aimin , Xiang Nan 
Published: January 2019

AbstractPrecise and reliable liquid delivery is vital for microfluidic applications. Here, we illustrate the design, fabrication, characterization, and application of a portable, low cost, and robust micropump, which brings solution to stable liquid delivery in microfluidic environment. The pump is designed with three optional speeds of different pumping flow rates, and it can be simply actuated by spring‐driven mechanism. The different flow rates of the pump are realized via passive microvalves in a compact microfluidic chip, which is installed in the pump. Importantly, the membrane structures of the microvalves allow accurate liquid control, and stable flow rates can be achieved via a spring setup. The proposed pump is applied to continuously and stably infuse microbead suspension into an inertial microfluidic chip, and good particle focusing is realized in the spiral channel of the inertial microfluidic chip. The proposed portable, self‐powered, and cost‐efficient pump is crucial for microfluidic lab‐on‐a‐chip system integration, which may facilitate microfluidic application for precise liquid delivery, control, measurement, and analysis.
Comparison of temperate and tropical freshwater species’ acute sensitivities to chemicals: An update
Authors: Wang Zhen , Kwok Kevin WH , Leung Kenneth MY 
Published: January 2019

Abstract

Toxicity data for tropical species are often lacking for deriving water quality guidelines (WQGs) and for conducting ecological risk assessment (ERA). To protect and safeguard valuable natural resources and important biodiversity in tropical freshwater ecosystems, a sound framework should be established to assess and manage the ecological risk of an ever-increasing number of chemicals that occur in the tropics. The present study aims to provide a more up-to-date comparison of the species sensitivity distributions (SSDs) between temperate and tropical freshwater species, by incorporating more acute toxicity data that have been documented. Results showed that temperate freshwater species are generally more sensitive to As, Cr, Pb, Hg, carbaryl, chlorpyrifos, DDT, lindane, and malathion than are their tropical counterparts, whereas tropical species tend to be more sensitive to un-ionized ammonia, Mn, chlordane, and phenol. No sensitivity differences were found between temperate and tropical freshwater species to Cu and pentachlorophenol. A general decline in sensitivity trend to chemicals was revealed by comparing taxon-specific SSDs, from crustaceans to mollusks, worms, fishes, and insects. On the basis of calculated 10% hazardous concentration (HC10) ratios from pairwise temperate and tropical SSDs, the temperate-to-tropic safe extrapolation factor was verified and refined as 5 for information. Integr Environ Assess Manag 2019;00:000–000. © 2019 SETAC
LncRNA MIAT facilitates osteosarcoma progression by regulating mir‐128‐3p/VEGFC axis
Authors: Zhang Chunyan , Xie Linsen , Liang Huiling , Cui Yuanbo 
Published: January 2019

AbstractThe aberrant expression of long non‐coding RNAs (lncRNAs) has been involved in the progression of many human tumors including osteosarcoma (OS). However, the biological function and the underlying mechanism of the lncRNA myocardial infarction‐associated transcript (MIAT) in OS remain unclear. In the present study, we found that lncRNA MIAT was significantly up‐regulated in both OS tissues and cell lines, and high expression of MIAT was positively associated with tumor size and lymph node metastasis of OS patients. In addition, knockdown of MIAT inhibited proliferation, migration, invasion and promoted apoptosis of OS cells in vitro. Moreover, the expression of MIAT was negatively associated with miR‐128‐3p but positively correlated with vascular endothelial growth factor C (VEGFC) in OS. Further mechanistic study revealed that lncRNA MIAT promoted OS progression by up‐regulating VEGFC via sponging miR‐128‐3p in vitro. Taken together, our results suggest that MIAT/miR‐128‐3p/VEGFC axis contributes to OS progression and may be used as a novel therapeutic target for OS. © 2019 IUBMB Life, 9999(9999):1–9, 2019
Going Global With Project‐Based Inquiry: Cosmopolitan Literacies in Practice
Authors: Spires Hiller A. , Himes Marie P. , Paul Casey Medlock , Kerkhoff Shea N. 
Published: January 2019

AbstractAs our world becomes increasingly interconnected, complex global challenges necessitate cross‐cultural collaborative efforts. Thus, developing cosmopolitan literacies among students and teachers becomes ever more important. Believing that cosmopolitan literacies are central to being literate in contemporary times, the authors build on their existing project‐based inquiry model to include global themes (e.g., poverty, global water and sanitation, climate change) and cross‐cultural exchange. This theory‐into‐practice article explains the Project‐Based Inquiry Global process and six design features that enable teachers to facilitate collaborative inquiry projects with their students. As students interact during the process, they begin to practice cosmopolitan literacies by engaging in reading, writing, and inquiry with people and topics from around the world, becoming cross‐cultural difference makers.
The outcomes of an impaired powerhouse in KRAS mutant lung adenocarcinoma cells by Elesclomol
Authors: Albayrak Gulsah , Korkmaz Funda Demirtas , Tozcu Duygu , Dogan Turacli Irem 
Published: January 2019

AbstractObjectivesLung cancer stands out as the most common cancer type worldwide. The most common genetic alteration detected in adenocarcinoma patients is KRAS. KRAS mutated patients still cannot get benefit from precision medicine approaches and lack a targeted therapy. Elesclomol is an investigational agent for melanoma and other malignancies. In this study, we evaluated its effect on cellular apoptosis, survival, and metastasis mechanisms on KRAS mutant A549 and Calu‐1 cell lines.MethodsThe cytotoxic effects of Elesclomol on A549 and Calu‐1 cells were determined by 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) cell viability test. Cells were treated with IC50 concentration and then apoptosis‐related (Casp‐3, Casp‐9, Bcl‐2, and Bcl‐xL), survival‐related (Akt, p‐Akt, Erk, and p‐Erk), and metastasis‐related (E‐cadherin, Vimentin, MMP‐2, and MMP‐9) protein expressions were determined by Western blot analysis. Elesclomol's effect on cell migration was evaluated by wound healing. Total oxidant, malondialdehyde (MDA), and glutathione (GSH) levels after Elesclomol treatment were assessed.ResultsElesclomol not only induced apoptotic proteins but also inhibited metastatic protein expressions and migration in both cells. Also, p‐Erk activity was diminished by Elesclomol treatment as a reflection of decreased proliferation. However, p‐Akt was enhanced as a cellular survival mechanism. Although Elesclomol's effects on oxidative stress parameters were puzzling, it induced total oxidant status (TOS), and MDA in Calu‐1 cells.ConclusionElesclomol might provide an alternative treatment approach for patients with KRAS mutant lung adenocarcinoma and other solid tumor malignancies that harbor KRAS mutations. This would enable the development of biomarker‐driven targeted therapy for KRAS mutant adenocarcinoma patients.
Role and practice evolution for genetic counseling in the genomic era: The experience of Australian and UK genetics practitioners
Authors: Dwarte Tanya , Barlow‐Stewart Kristine , O’Shea Rosie , Dinger Marcel E. , Terrill Bronwyn 
Published: January 2019

AbstractFacilitating informed decision‐making regarding genetic testing is a core component of genetic counseling practice. Internationally, genetic testing is shifting toward gene panels and genomic testing, including whole exome and whole genome sequencing to improve diagnostic yield and cost‐effectiveness. This study explored genetics practitioners’ current experience with panels and genomic tests and the associated evolution of genetic counseling practice. Genetics practitioners with genomic testing experience, were purposively invited to participate in a semi‐structured telephone interview and to snowball the invitation to colleagues. Interviews conducted with participants residing in Australia (n = 9) and the UK (n = 5) were transcribed and analyzed using an inductive thematic approach. Three themes emerged: (a) Role delineation: current roles, future roles, and the influence of increasing complexity; (b) The evolving spectrum of practice: blurred boundaries between research and clinical services; impact on facilitation of informed consent; and return of results strategies; and (c) Policy and governance needs: equality of access; achieving consistent variant interpretation, reporting, and responsibility for review; managing incidental findings; and professional regulation for Australian genetic counselors. These exploratory data highlight that genetic counseling practice and the essential role of facilitating informed consent are evolving but remain patient‐centered, with core skills underpinning practitioners’ capacity to adapt.
Use of ultra‐performance liquid chromatography–tandem mass spectrometry on sweet cherries to determine phenolic compounds in peel and flesh
Authors: Gao Yuan , Wang Meng , Jiang Nan , Wang Yao , Feng Xiaoyuan 
Published: January 2019

AbstractBACKGOUNDSweet cherries (Prunus aviumL.) are rich in polyphenols and have high antioxidant potential. This study deals with the use of ultra‐performance liquid chromatography–tandem mass spectrometry on five cherry cultivars, namely Caihong, Sunburst, Summit, 23–51 and Valeri, to determine the free, conjugated and bound phenolics in peel and flesh.RESULTSPhenolics varied widely based on cherry cultivars and edible parts. Valeri showed the highest phenolics in peel, while the flesh of 23–51 contained a higher amount of phenolic substances compared with the other cherries, mainly due to high levels of cyanidin‐3‐O‐rutinoside, which was the most abundant phenolic in sweet cherry. Most of the phenolics, except for ferulic acid and vanillic acid, were present at a higher level in peel than in flesh. Principal component analysis indicated that compared with different edible parts, the phenolic compounds were more influenced by cultivar.CONCLUSIONSCultivar had a much greater impact on the accumulation of phenolics than edible parts. The peel of Valeri contained the highest phenolics; for the flesh, 23–51 had the highest levels. The high variability in phenolic compounds in peel and flesh among cultivars may be useful for breeding and underlines the importance of germplasm conservation activities. © 2019 Society of Chemical Industry
Chemical–nutritional quality and oxidative stability of milk and dairy products obtained from Friesian cows fed with a dietary supplementation of dried grape pomace
Authors: Ianni Andrea , Di Maio Gaetano , Pittia Paola , Grotta Lisa , Perpetuini Giorgia , Tofalo Rosanna , Cichelli Angelo , Martino Giuseppe 
Published: January 2019

ABSTRACTBACKGROUNDThe aim of the study was to evaluate the nutritional properties of milk and cheese obtained from Friesian cows fed with a diet supplemented with dried grape pomace, a by‐product of the oenological industry, which is very rich in polyphenols. This approach is inspired by the increasing interest in foods containing functional ingredients that may have beneficial effects on human health. During the testing period, analyses of the chemical and nutritional properties of milk and dairy products derived from it were performed; particular attention was given to the effect of cheese ripening on the oxidative stability and fermentation process, evaluating respectively the presence of malondialdehyde and γ‐aminobutyric acid.RESULTSDietary enrichment with grape pomace did not affect the milk composition but induced modifications in the fatty acid profiles in both milk and cheese with an increase in concentration of linoleic acid, trans‐vaccenic acid, rumenic acid and total n‐6 fatty acids. Moreover, after 30 days of cheese ripening, an increased oxidative stability and an increased concentration of γ‐aminobutyric acid were found.CONCLUSIONSOur results indicated a general improvement in nutritional parameters of milk and related cheese obtained from Friesian cows that received the feeding enrichment with dried grape pomace. Further analysis should be performed to improve knowledge of the chemical and microbiological mechanisms at the source of these findings. © 2019 Society of Chemical Industry