bluuebunny/crossref_metadata_2025_split huggingface dataset:
Whole exome sequencing identified a novel mutation (p.Ala1884Pro) of β‐spectrin in a Chinese family with hereditary spherocytosis
Authors: Fan Liang‐Liang , Liu Ji‐Shi , Huang Hao , Du Ran , Xiang Rong 
Published: January 2019

AbstractBackgroundHereditary spherocytosis (HS) is an inherited disorder of erythrocyte. The typical feature of HS is the presence of spherical‐shaped erythrocytes on the peripheral blood smear. According to previous studies, more than five candidate genes, such as ANK1, SPTB, SPTA1, SLC4A1 and EPB42 have been identified in HS patients.MethodsIn the present study, a Chinese HS family was investigated. The proband suffered from pathologic jaundice and splenomegaly. A blood test and peripheral blood smear experiment further confirmed the diagnosis of HS. We selected the proband to perform the whole exome sequencing.ResultsAfter data filtering and co‐segregation analysis, we identified 12 mutations in affected members that were absent in healthy members. In consideration of the inheritance pattern, Online Mendelian Inheritance in Man clinical phenotypes, Toppgene function and American College of Medical Genetics classification, we considered the novel mutation (c.5650G > C/p.Ala1884Pro) of β‐spectrin (SPTB) to be the genetic lesion in this family. The novel mutation, resulting in a substitution of alanine by proline, may lead to transformation of the SPTB protein structure, which affects the binding between SPTB and ankyrin.ConclusionsThe present study confirmed the hereditary red blood cell membrane disorders at a molecular level and expanded the spectrum of SPTB mutations. This may contribute to the clinical management and genetic counseling with respect to HS.
Synthesis and Insecticidal Activity of New Quinoline Derivatives Containing Perfluoropropanyl Moiety
Authors: Cheng Long , Cai Peng‐Peng , Zhang Rui‐Rui , Han Liang , Tan Cheng‐Xia , Weng Jian‐Quan , Xu Tian‐Ming , Liu Xing‐Hai 
Published: January 2019

A series of new quinoline derivatives containing perfluoropropanyl moiety were designed and synthesized. Their structures were confirmed by 1H‐NMR, MS, and elemental analysis. The bioassay results showed that compound 4e exhibited good insecticidal activity against Plutella xylostella and Mythimna separata at 100 mg/L.
Focal cerebellar infarction as an initial sign of mitochondrial myopathy, encephalopathy, lactic acidosis, and stroke‐like episodes
Authors: Ko Ara , Lee Sang‐Jun , Lee Young‐Mock 
Published: January 2019

AbstractMitochondrial myopathy, encephalopathy, lactic acidosis, and stroke‐like episodes (MELAS) is a type of mitochondrial disorder and stroke‐like lesions are observed prominently in the brain magnetic resonance imaging. Those stroke‐like lesions of MELAS patients are usually located in the posterior quadrants and do not correspond to typical vascular territories. This case illustrates that focal cerebellar infarction can be the sole initial sign of MELAS.
3D‐T1ρ prepared zero echo time‐based PETRA sequence for in vivo biexponential relaxation mapping of semisolid short‐T2 tissues at 3 T
Authors: Sharafi Azadeh , Baboli Rahman , Chang Gregory , Regatte Ravinder R. 
Published: January 2019

BackgroundIn addition to the articular cartilage, osteoarthritis (OA) affects several other tissues such as tendons, ligaments, and subchondral bone. T1ρ relaxation study of these short T2 tissues may provide a more comprehensive evaluation of OA.PurposeTo develop a 3D spin‐lattice relaxation in the rotating frame (T1ρ) prepared zero echo time (ZTE)‐based pointwise encoding time reduction with radial acquisition (3D‐T1ρ‐PETRA) sequence for relaxation mapping of semisolid short‐T2 tissues on a clinical 3 T scanner.Study TypeProspective.PopulationPhantom, two bovine whole knee joint and Achilles tendon specimens, 10 healthy volunteers with no known inflammation, trauma or pain in the knee or ankle.Field Strength/SequenceA customized PETRA sequence to acquire fat‐suppressed 3D T1ρ‐weighted images tissues with semisolid short T2/ relaxation times in the knee and ankle joints at 3 T.AssessmentMono‐ and biexponential T1ρ relaxation components were assessed in the patellar tendon (PT), anterior cruciate ligament (ACL), posterior cruciate ligament (PCL), and Achilles tendon (AT).Statistical TestsKruskal–Wallis with post‐hoc Dunn's test for multiple pairwise comparisons.ResultsPhantom and ex vivo studies showed the feasibility of T1ρ relaxation mapping using the proposed 3D‐T1ρ‐PETRA sequence. The in vivo study demonstrated an averaged mono‐T1ρ relaxation of (median [IQR]) 15.9 [14.5] msec, 23.6 [9.4] msec, 17.4 [7.4] msec, and 5.8 [10.2] msec in the PT, ACL, PCL, and AT, respectively. The bicomponent analysis showed the short and long components (with their relative fractions) of 0.65 [1.0] msec (46.9 [15.3]%) and 37.3 [18.4] msec (53.1 [15.3]%) for PT, 1.7 [2.1] msec (42.5 [12.5]%) and 43.7 [17.8] msec (57.5 [12.5]%) for ACL, and 1.2 [1.9] msec (42.6 [14.0]%) and 27.7 [14.7] msec (57.3 [14.0]%) for PCL and 0.4 [0.02] msec (58.8 [13.3]%/) and 31.3 [10.8] msec (41.2 [13.3]%) for AT. Statistically significant (P ≤ 0.05) differences were observed in the mono‐ and biexponential relaxation between several regions.Data ConclusionThe 3D‐T1ρ‐PETRA sequence allows volumetric, isotropic (0.78 × 0.78 × 0.78 mm), biexponential T1ρ assessment with corresponding fractions of the tissues with semisolid short T2/.Level of Evidence: 2Technical Efficacy Stage: 1J. Magn. Reson. Imaging 2019;50:1207–1218.
Histatin‐1 counteracts the cytotoxic and antimigratory effects of zoledronic acid in endothelial and osteoblast‐like cells
Authors: Castro Martín , Torres Pedro , Solano Luis , Córdova Luis A. , Torres Vicente A. 
Published: January 2019

AbstractBackgroundZoledronic acid, the most frequent agent associated with bisphosphonate‐related osteonecrosis of the jaw (BRONJ), has been reported as cytotoxic for bone and vascular cells. Hence, identification of novel approaches aiming to counteract its cytotoxic effects will be desirable to develop preventive therapies for BRONJ. The salivary peptide Histatin‐1 was recently shown to promote oral wound healing, by acting in epithelial and endothelial cells; however, its effects on cells exposed to zoledronic acid have not been explored. This study aims to unveil the role of Histatin‐1 in osteoblastic and vascular cell lineages challenged with zoledronic acid.MethodsThe effects of zoledronic acid (1–100 µM), Histatin‐1 (10 µM), or their combination was evaluated in cytotoxicity (Trypan Blue exclusion) and cell migration (Boyden Chamber) assays. Caspase‐3 cleavage was evaluated by Western blot. The angiogenic capacity of endothelial cells was assessed in a tubule formation assay in vitro.ResultsZoledronic acid decreased cell viability and migration of osteosarcoma cells (SAOS‐2) and preosteoblasts (MC3T3‐E1), in a dose‐response manner. Importantly, Histatin‐1 restored both cell viability and migration in both cell lines upon challenge with zoledronic acid. These effects were recapitulated in endothelial cells (EA.hy926), as Histatin‐1 counteracted cytotoxic and antimigratory effects of zoledronic acid, and restored the angiogenic capacity in vitro.ConclusionWe conclude that Histatin‐1 counteracts the cytotoxic and antimigratory effects of zoledronic acid in osteoblast‐like and endothelial cells. These observations highlight the potential use of Histatin‐1, in the design of novel therapies aiming to prevent and treat BRONJ.
Effect of frying and storage on oxidative quality of conjugated linoleic‐acid‐rich soybean oil produced by photoisomerization using plantain as a model system
Authors: Rodríguez‐Miranda Jesus , Gallegos‐Marín Ivet , Hernández‐Santos Betsabe , Herman‐Lara Erasmo , Medina‐Juárez Luis Angel , Juárez‐Barrientos José M , Martínez‐Sánchez Cecilia E 
Published: January 2019

AbstractBACKGROUNDConjugated linoleic acid (CLA) is known to have beneficial properties to health. Naturally, in foods it is found in very low concentrations, and so these beneficial properties cannot be obtained. This study investigated the enrichment of soybean oil by photoisomerization, as well as assessing its oxidative stability during the frying process using plantain slices as a model system and after a storage period of 20 days at 60 °C.RESULTSThe oxidative stability of soybean oil enriched with CLA by photoirradiation was measured based on the peroxide, p‐anisidine, and Totox values, as well as by the polyphenol content, tocopherol content and DPPH· scavenging capacity. The results obtained showed that a substantial amount of CLA was obtained by photoirradiation (31.73%). The oxidative stability values of the oil enriched with CLA showed good stability during a frying cycle; however, this stability decreased when it was stored and during the final frying cycles.CONCLUSIONSThe results obtained indicated that photoirradiation is a good technique for obtaining oils enriched with CLA, and in this way CLA can be incorporated into foods; however, it is necessary to add antioxidants to improve their stability. © 2019 Society of Chemical Industry
Nonsuicidal Self‐Injury and Borderline Personality Features as Risk Factors for Suicidal Ideation Among Male Veterans With Posttraumatic Stress Disorder
Authors: Cunningham Katherine C. , Grossmann Jessica L. , Seay Kathryn B. , Dennis Paul A. , Clancy Carolina P. , Hertzberg Michael A. , Berlin Kate , Ruffin Rachel A. , Dedert Eric A. , Gratz Kim L. , Calhoun Patrick S. , Beckham Jean C. , Kimbrel Nathan A. 
Published: January 2019

AbstractU.S. veterans are at increased risk for suicide compared to their civilian counterparts and account for approximately 20% of all deaths by suicide. Posttraumatic stress disorder (PTSD) and borderline personality features (BPF) have each been associated with increased suicide risk. Additionally, emerging research suggests that nonsuicidal self‐injury (NSSI) may be a unique risk factor for suicidal behavior. Archival data from 728 male veterans with a PTSD diagnosis who were receiving care through an outpatient Veterans Health Administration (VHA) specialty PTSD clinic were analyzed. Diagnosis of PTSD was based on a structured clinical interview administered by trained clinicians. A subscale of the Personality Assessment Inventory was used to assess BPF, and NSSI and suicidal ideation (SI) were assessed by self‐report. Findings revealed that NSSI (58.8%) and BPF (23.5%) were both relatively common in this sample of male veterans with PTSD. As expected, each condition was associated with significantly increased odds of experiencing SI compared to PTSD alone, odds ratios (ORs) = 1.2–2.6. Moreover, co‐occurring PTSD, NSSI, and BPF were associated with significantly increased odds of experiencing SI compared with PTSD, OR = 5.68; comorbid PTSD and NSSI, OR = 2.57; and comorbid PTSD and BPF, OR = 2.13. The present findings provide new insight into the rates of NSSI and BPF among male veterans with PTSD and highlight the potential importance of these factors in suicide risk.
Doppler Color Scoring System in Women With an Incomplete Miscarriage: Interobserver and Intraobserver Reproducibility Study
Authors: Leong Grace Ting Ting , Leonardi Mathew , Lu Chuan , Mein Brendan , Espada Mercedes , Shakeri Babak , Nadim Batool , Reid Shannon , Casikar Ishwari , Condous George 
Published: January 2019

ObjectivesDoppler Color Scoring (DCS) has been used to predict successful expectant management of incomplete miscarriage. The aim of this study was to assess inter‐ and intraobserver reproducibility of the DCS system in women with incomplete miscarriage noted on transvaginal sonography.MethodsThis was a prospective reproducibility study involving offline analysis of 32 prerecorded video sets on transvaginal sonography in real time of women with incomplete miscarriage. Vascularization of retained products of conception was recorded using the DCS system adopted from the International Ovarian Tumor Analysis group. Five gynecologic sonologists of varying experience assigned a DCS classification to each video in the analysis. The same videos were reanalyzed, in a different order, at least 7 days later, to assess intraobserver agreement. Inter‐ and intraobserver correlations were performed to determine agreement. Interobserver agreement was also measured between each observer and the reference standard (G.C.). A Cohen's κ coefficient value less than 0 suggests poor agreement, 0.01 to 0.20 slight, 0.21 to 0.40 fair, 0.41 to 0.60 moderate, 0.61 to 0.80 substantial, and 0.81 and 0.99 almost perfect.ResultsInterobserver agreement for all observers for DCS allocation ranged from 0.480 to 0.751. Overall interobserver agreement for 5 observers was substantial (κ, 0.626). Overall interobserver agreements for the 2 inexperienced and 3 experienced observers compared to G.C. were 0.521 and 0.618, respectively. Experienced observers achieved overall almost perfect intraobserver agreement, compared to substantial agreement for inexperienced sonologists.ConclusionsDCS interobserver reproducibility between all observers and GC ranged from moderate to substantial. DCS intraobserver reproducibility was substantial to almost perfect. The DCS system appears to be a reproducible tool in evaluating women with incomplete miscarriage.
NPK 2.0: Introducing tensor decompositions to the kinetic analysis of gas–solid reactions
Authors: Birkelbach Felix , Deutsch Markus , Flegkas Stylianos , Winter Franz , Werner Andreas 
Published: January 2019

AbstractA method for deriving kinetic models of gas–solid reactions for reactor and process design is presented. It is based on the nonparametric kinetics (NPK) method and resolves many of its shortcomings by applying tensor rank‐1 approximation methods. With this method, it is possible to derive kinetic models based on the general kinetic equation from any combination of experiments without additional a priori assumptions. The most notable improvements over the original method are that it is computationally much simpler and that it is not limited to two variables. Two algorithms for computing the rank‐1 approximation as well as a tailored initialization method are presented, and their performance is assessed. Formulae for the variance estimation of the solution values are derived to improve the accuracy of the model identification and to provide a tool for diagnosing the quality of the kinetic model. The methods effectiveness and performance are assessed by applying it to a simulated data set. A Matlab implementation is available as Supporting Information.
Qualitative and quantitative scintigraphy in sialorrhea before and after botulinum toxin injection
Authors: Ribeiro Livia Barroso , Xerez Denise Rodrigues , Maliska Carmelindo , de Souza Sergio Augusto Lopes , Costa Milton Melciades Barbosa 
Published: January 2019

Objectives/HypothesisSialorrhea is excessive saliva production and its usual escape of from the oral cavity. The use of botulinum toxin has been preconized, but its effectiveness until now has been unreliably measured. Our objective was to qualitatively and quantitatively determine the effectiveness of botulinum toxin injection in the reduction of saliva production by the parotid gland.Study DesignOutcomes research.MethodsPatients with moderate‐to‐critical sialorrhea had one of the parotid glands injected with 50 U of botulinum toxin, leaving the other as the control. Fifteen days after the toxin injection, they underwent scintigraphic analyses with intravenous injection of 10 mCi (37 MBq) of Tc‐99 m (sodium pertechnetate). After this, the noninjected gland was treated for therapeutic complementation.ResultsThe glands injected with botulinum toxin showed uptake reduction in 100% of patients. The uptake reduction in counts per second varied from 8% to 36%. The Wilcoxon paired test comparing the control glands with those injected showed a significant difference for the action of botulinum toxin (P = .0039).ConclusionsThe scintigraphic study of parotid glands shows that botulinum toxin is effective in reducing sodium pertechnetate uptake.Level of Evidence2c Laryngoscope, 129:2521–2526, 2019
Factors associated with suicidal ideation risk in head and neck cancer: A longitudinal study
Authors: Chang Dun‐Cheng , Chen Andy Wei‐Ge , Lo Yu‐Sheng , Chuang Yi‐Ching , Chen Mu‐Kuan 
Published: January 2019

Objectives/HypothesisThe risk factors associated with suicidal ideation among patients with head and neck cancer (HNC) have rarely been investigated. Thus, this study proposed an effective screening model to facilitate the identification of patients with HNC who are at risk of committing suicide.Study DesignRetrospective longitudinal study.MethodsWe recruited 286 inpatients with HNC who were referred to the psychiatry department for an emotional distress assessment during cancer treatment. Sociodemographic and clinical factors, as well as Distress Thermometer (DT) scoring, were gathered and analyzed using multivariate logistic regression.ResultsAmong the patients, 27 (9.4%) were recognized as having suicidal ideation. A logistic regression analysis revealed five significant indicators associated with suicidal ideation, namely depression (odds ratio [OR]: 42.83), dysphoria (OR: 8.06), hypopharyngeal cancer (OR: 4.61), cancer history (OR: 4.53), and average DT scores (OR: 1.84). Based on the receiver operating characteristic and logistic regression, the model can predict suicidal ideation with 93.4% accuracy (area under the curve value).ConclusionsOur findings provide strong evidence that patients with depression, hypopharyngeal cancer, higher DT scores, and previous cancer history can be quickly screened for suicidal ideation, which may help to prevent suicide events in patients with HNC.Level of Evidence3b Laryngoscope, 129:2491–2495, 2019
Biogeographic drivers of diazotrophs in the western Pacific Ocean
Authors: Chen Mingming , Lu Yangyang , Jiao Nianzhi , Tian Jiwei , Kao Shuh‐Ji , Zhang Yao 
Published: January 2019

AbstractThe global budget of marine nitrogen (N) is not balanced, with N removal largely exceeding N fixation. One of the major causes of this imbalance is our inadequate understanding of the diversity and distribution of marine N2 fixers (diazotrophs) as well as their contribution to N2 fixation. Here, we performed a large‐scale cross‐system study spanning the South China Sea, Luzon Strait, Philippine Sea, and western tropical Pacific Ocean to compare the biogeography of seven major diazotrophic groups and N2 fixation rates in these ecosystems. Distinct spatial niche differentiation was observed. Trichodesmium was dominant in the South China Sea and western equatorial Pacific, whereas the unicellular cyanobacterium UCYN‐B dominated in the Philippine Sea. Furthermore, contrasting diel patterns of Trichodesmium nifH genes and UCYN‐B nifH gene transcript activity were observed. The heterotrophic diazotroph Gamma A phylotype was widespread throughout the western Pacific Ocean and occupied an ecological niche that overlapped with that of UCYN‐B. Moreover, Gamma A (or other possible unknown/undetected diazotrophs) rather than Trichodesmium and UCYN‐B may have been responsible for the high N2 fixation rates in some samples. Regional biogeochemistry analyses revealed cross‐system variations in N2‐fixing community composition and activity constrained by sea surface temperature, aerosol optical thickness, current velocity, mixed‐layer depth, and chlorophyll a concentration. These factors except for temperature essentially control/reflected iron supply/bioavailability and thus drive diazotroph biogeography. This study highlights biogeographical controls on marine N2 fixers and increases our understanding of global diazotroph biogeography.
Multiparametric MR for non‐invasive evaluation of tumour tissue histological characteristics after radionuclide therapy
Authors: Montelius Mikael , Jalnefjord Oscar , Spetz Johan , Nilsson Ola , Forssell‐Aronsson Eva , Ljungberg Maria 
Published: January 2019

Early non‐invasive tumour therapy response assessment requires methods sensitive to biological and physiological tumour characteristics.The aim of this study was to find and evaluate magnetic resonance imaging (MRI) derived tumour tissue parameters that correlate with histological parameters and that reflect effects of radionuclide therapy.Mice bearing a subcutaneous human small‐intestine neuroendocrine tumour were i.v. injected with 177Lu‐octreotate. MRI was performed (7 T Bruker Biospec) on different post‐therapy intervals (1 and 13 days) using T2‐weighted imaging, mapping of T2* and T1 relaxation time constants, as well as diffusion and dynamic contrast enhancement (DCE‐MRI) techniques. After MRI, animals were killed and tumours excised. Four differently stained histological sections of the most central imaged tumour plane were digitized, and segmentation techniques were used to produce maps reflecting fibrotic and vascular density, apoptosis, and proliferation. Histological maps were aligned with MRI‐derived parametric maps using landmark‐based registration. Correlations and predictive power were evaluated using linear mixed‐effects models and cross‐validation, respectively.Several MR parameters showed statistically significant correlations with histological parameters. In particular, three DCE‐MRI‐derived parameters reflecting capillary function additionally showed high predictive power regarding apoptosis (2/3) and proliferation (1/3). T1 could be used to predict vascular density, and perfusion fraction derived from diffusion MRI could predict fibrotic density, although with lower predictive power.This work demonstrates the potential to use multiparametric MRI to retrieve important information on the tumour microenvironment after radiotherapy. The non‐invasiveness of the method also allows longitudinal tumour tissue characterization. Further investigation is warranted to evaluate the parameters highlighted in this study longitudinally, in larger studies, and with additional histological methods.
BOLD‐based cerebrovascular reactivity vascular transfer function isolates amplitude and timing responses to better characterize cerebral small vessel disease
Authors: Atwi Sarah , Shao Han , Crane David E. , da Costa Leodante , Aviv Richard I. , Mikulis David J. , Black Sandra E. , MacIntosh Bradley J. 
Published: January 2019

Cerebrovascular reactivity (CVR) is a dynamic measure of the cerebral blood vessel response to vasoactive stimulus. Conventional CVR measures amplitude changes in the blood‐oxygenation‐level‐dependent (BOLD) signal per unit change in end‐tidal CO2 (PETCO2), effectively discarding potential timing information. This study proposes a deconvolution procedure to characterize CVR responses based on a vascular transfer function (VTF) that separates amplitude and timing CVR effects. We implemented the CVR‐VTF to primarily evaluate normal‐appearing white matter (WM) responses in those with a range of small vessel disease. Comparisons between simulations of PETCO2 input models revealed that boxcar and ramp hypercapnia paradigms had the lowest relative deconvolution error. We used a T2* BOLD‐MRI sequence on a 3 T MRI scanner, with a boxcar delivery model of CO2, to test the CVR‐VTF approach in 18 healthy adults and three white matter hyperintensity (WMH) groups: 20 adults with moderate WMH, 12 adults with severe WMH, and 10 adults with genetic WMH (CADASIL). A subset of participants performed a second CVR session at a one‐year follow‐up. Conventional CVR, area under the curve of VTF (VTF‐AUC), and VTF time‐to‐peak (VTF‐TTP) were assessed in WM and grey matter (GM) at baseline and one‐year follow‐up. WMH groups had lower WM VTF‐AUC compared with the healthy group (p < 0.0001), whereas GM CVR did not differ between groups (p > 0.1). WM VTF‐TTP of the healthy group was less than that in the moderate WMH group (p = 0.016). Baseline VTF‐AUC was lower than follow‐up VTF‐AUC in WM (p = 0.013) and GM (p = 0.026). The intraclass correlation for VTF‐AUC in WM was 0.39 and coefficient of repeatability was 0.08 [%BOLD/mm Hg]. This study assessed CVR timing and amplitude information without applying model assumptions to the CVR response; this approach may be useful in the development of robust clinical biomarkers of CSVD.