bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pilot study of a mobile smoking cessation intervention for low-income smokers with serious mental illness
Authors: Brunette Mary F. , Ferron Joelle C. , Geiger Pamela , Guarino Susan , Pratt Sarah I. , Lord Sarah E. , Aschbrenner Kelly A. , Adachi-Mejia Anna 
Published: June 2019

AbstractIntroductionPeople with serious mental illness (SMI) have high rates of smoking and need better access to cessation treatment. Mobile behavioral interventions for cessation have been effective for the general population, but are not usable by many with SMI due to cognitive impairments or severe symptoms. We developed a tailored mobile cessation treatment intervention with features to reduce cognitive load.MethodWe enrolled 20 smokers with SMI and showed them how to use the program on a device of their choice. They were assessed at 8 weeks for intervention use, usability, satisfaction, smoking characteristics, and biologically verified abstinence.ResultsParticipants accessed an average of 23.6 intervention sessions (SD = 17.05; range 1–48; median = 17.5) for an average total of 231.64 minutes (SD = 227.13; range 4.89–955.21; median = 158.18). For 87% of the sessions, average satisfaction scores were 3 or greater on a scale of 1–4. Regarding smoking, 25% of participants had reduced their smoking and 10% had biologically verified abstinence from smoking at 8 weeks.ConclusionHome and community use of this mobile cessation intervention was feasible among smokers with SMI. Further research is needed to evaluate such scalable approaches to increase access to behavioral treatment for this group.
A method for the measurement of vitamin B12concentration in sea water
Authors: Daisley K. W. 
Published: June 2019

The vitamin B12concentration in sea water is so very low that only the most sensitive microbiological assay techniques are likely to be capable of measuring it, but the salt concentration is so high as to be inhibitory to most of the organisms conventionally used for the microbiological assay of the vitamin. Nevertheless, the consideration that vitamin B12concentration may have an influence on the amounts and types of life present in sea areas has persuaded several investigators to attempt its measurement.The methods so far employed have each their own advantages and dis-advantages (Droop, 1954, 1955; Lewin, 1954; Sweeney, 1954; Cowey, 1956; Adair & Vishniac, 1958). The method to be described here offers advantages in sensitivity, permitting direct measurement of the vitamin in small quantities of oceanic waters after further dilution; samples to be measured pass through comparatively few different containers and thus there is less risk of their accumulating adventitious vitamin; simple and easily cleaned apparatus is used, and much less bench-work is involved than when methods other than dilution are used for lowering the salt concentration.
VI.—The Geochemistry of the Rosses Granitic Ring Complex, Co. Donegal, Ireland
Authors: Mercy Edward L. P. 
Published: June 2019

SynopsisThe members of the Rosses Granitic Ring Complex have been chemically analyzed. The data are presented as averages with 95 per cent confidence limits for individual elements. A limited amount of crystallization differentiation is demonstrated when the major units—the biotite microgranite sheets, the porphyry dykes, the ring granites and the grey and pink microgranite sheets—are compared with one another. This involves an increase in Si, K, a decrease in Ti, Al, Fe, Mg, Ca and P, but Na (and Mn) remain constant. The ring granites G1-G4 undergo very little differentiation and variations in their composition, except for Si, are ascribed mainly to heterogeneity.The origin of the magma which gave rise to the complex is due to the palingenesis of the Older Granodiorite under the influence of a Na, K, alumino-silicate influx. This magma was the source of the biotite microgranite sheets and, after differentiation, the granites G1 and G2, together with some of the grey and pink microgranite sheets. After a period of quiescence a second influx produced the similar magma which was the source of the porphyry dykes and, after differentiation, gave rise to the granites G3 and G4, the Newer Granite bosses and the late grey and pink microgranite sheets.
XI.—The Silurian Inlier of Carmichael, Lanarkshire
Authors: Rolfe W. D. Ian 
Published: June 2019

SynopsisThe occurrence of Monograptus in the Carmichael Burn section of the Midland Valley of Scotland has now been confirmed. A new fauna is described from beds previously regarded as Ludlovian which are here suggested to be of Valentian age. A detailed stratigraphical succession has been established and the structure of the area revised in the light of this new information, most of which was derived from a number of exploratory pits sunk for the purpose. Tentative correlations with the other Midland Valley inliers are attempted and many problems requiring future solution are raised.
XIII.—On the Structure of Deltasperma fouldenense gen. et sp. nov., and Camptosperma berniciense gen. et sp. nov., petrified seeds from the Calciferous Sandstone Series of Berwickshire
Authors: Long Albert G. 
Published: June 2019

SynopsisTwo new species of fossil seeds are described from the Cementstone Group of Berwickshire. Deltasperma fouldenense is asymmetrically platyspermic, and has large superficial cells chiefly on the more convex side. It is orthotropous but shows no pedicel. Camptosperma berniciense is campylotropous and has a dense integument. A vascular bundle to the chalaza ends at the nucellus base.Both seeds possess a similar lagenostome and short salpinx; two integumental bundles which expand in the free lobes; a female prothallus with a wide “tent-pole” and about two archegonia; a nucellus adnate to the integument below the lagenostome; and spherical pollen grains in the pollen chamber. The seeds show most affinity with the Lagenostomales.
XV.—Some Pteridosperm Seeds from the Calciferous Sandstone Series of Berwickshire
Authors: Long Albert G. 
Published: June 2019

SynopsisFour new species of petrified seeds are described:1.Eccroustosperma langtonense gen. et sp. nov.—a curved platyspermic seed with two apical lobes of the integument and a rough sclerotesta when mature.2.Hydrasperma tenuis gen. et sp. nov.—a small slender seed with 8–10 free apical lobes and longitudinal external ridges.3.Stamnostoma bifrons sp. nov.—a barrel-shaped seed with a nucellar apex and micropyle intermediate in form between Stamnostoma huttonense and Sphcerostoma ovale.4.Dolichosperma sexangulatum gen. et sp. nov.—a relatively long narrow radiospermic seed with a dense sixangled integument open above and without tentacles or micropyle. The salpinx was probably funnel-shaped.All four species are assigned to the Lagenostomales.
Strongly Coupled Data Assimilation in Multiscale Media: Experiments Using a Quasi‐Geostrophic Coupled Model
Authors: Penny S. G. , Bach E. , Bhargava K. , Chang C.‐C. , Da C. , Sun L. , Yoshida T. 
Published: June 2019

AbstractStrongly coupled data assimilation (SCDA) views the Earth as one unified system. This allows observations to have an instantaneous impact across boundaries such as the air‐sea interface when estimating the state of each individual component. Operational prediction centers are moving toward Earth system modeling for all forecast timescales, ranging from days to months. However, there have been few studies that examine fundamental aspects of SCDA and the transition from traditional approaches that apply data assimilation only to a single component, whether forecasts were derived from a coupled model or an uncoupled forced model. The SCDA approach is examined here in detail using numerical experiments with a simple coupled atmosphere‐ocean quasi‐geostrophic model. The impact of coupling is explored with respect to its impact on the Lyapunov spectrum and on data assimilation system stability. Different data assimilation methods are compared within the context of SCDA, including the 3‐D and 4‐D Variational methods, the ensemble Kalman filter, and the hybrid gain method. The impact of observing system coverage is also investigated. We find that SCDA is generally superior to weakly coupled or uncoupled approaches. Dynamically defined background error covariance estimates are essential for SCDA to achieve an accurate coupled state estimate as the observing system becomes sparser. As a clarification of seemingly contradictory findings from previous studies, it is shown that ocean observations can adequately constrain atmospheric state estimates provided that the analysis‐observing frequency is sufficiently high and the ensemble size determining the background error covariance is sufficiently large.
Fibrotic liver microenvironment promotes Dll4 and SDF-1-dependent T-cell lineage development
Authors: Gong Zheng , Shang Bingxue , Chu Yunpeng , Chen Xiaodong , Li Qing , Liu Keli , Chen Yongjing , Huang Yin , Han Yanyan , Shang Qianwen , Zheng Zhiyuan , Song Lin , Li Yanan , Liu Rui , Xu Chenchang , Zhang Xiaoren , Liu Baochi , Wang Luowei , Shao Changshun , Wang Ying , Shi Yufang 
Published: June 2019

AbstractThe reconstitution of the T-cell repertoire and quantity is a major challenge in the clinical management of HIV infection/AIDS, cancer, and aging-associated diseases. We previously showed that autologous bone marrow transfusion (BMT) via the hepatic portal vein could effectively restore CD4+T-cell count in AIDS patients also suffering from decompensated liver cirrhosis. In the current study, we characterized T-cell reconstitution in a mouse model of liver fibrosis induced by CCl4and found that T-cell reconstitution after BMT via hepatic portal vein was also greatly enhanced. The expression ofDll4(Delta-like 4), which plays an important role in T-cell progenitor expansion, was elevated in hepatocytes of fibrotic livers when compared to normal livers. This upregulation ofDll4expression was found to be induced by TNFα in an NFκB-dependent manner. Liver fibroblasts transfected with Dll4 (LF-Dll4) also gained the capacity to promote T-cell lineage development from hematopoietic stem cells (HSCs), resulting in the generation of DN2 (CD4 and CD8 DN 2) and DN3 T-cell progenitors in vitro, which underwent a normal maturation program when adoptively transferred intoRag-2deficient hosts. We also demonstrated a pivotal role of SDF-1 produced by primary liver fibroblasts (primary LF) in T-lineage differentiation from HSCs. These results suggest that Dll4 and SDF-1 in fibrotic liver microenvironment could promote extrathymic T-cell lineage development. These results expand our knowledge of T-cell development and reconstitution under pathological conditions.
H7N9 influenza A virus activation of necroptosis in human monocytes links innate and adaptive immune responses
Authors: Lee Andrew C. Y. , Zhang Anna J. X. , Chu Hin , Li Can , Zhu Houshun , Mak Winger W. N. , Chen Yanxia , Kok Kin-Hang , To Kelvin K. W. , Yuen Kwok-Yung 
Published: June 2019

AbstractWe previously demonstrated that avian influenza A H7N9 virus preferentially infected CD14+ monocyte in human peripheral blood mononuclear cells (PBMCs), which led to apoptosis. To better understand H7N9 pathogenesis in relation to monocyte cell death, we showed here that extensive phosphorylation of mixed lineage kinase domain-like (MLKL) protein occurred concurrently with the activation of caspases-8, -9 and -3 in H7N9-infected monocytes at 6 h post infection (hpi), indicating that apoptosis and necroptosis pathways were simultaneously activated. The apoptotic morphology was readily observed in H7N9-infected monocytes with transmission electron microscopy (TEM), while the pan-caspase inhibitor, IDN6556 (IDN), accelerated cell death through necroptosis as evidenced by the increased level of pMLKL accompanied with cell swelling and plasma membrane rupture. Most importantly, H7N9-induced cell death could only be stopped by the combined treatment of IDN and necrosulfonamide (NSA), a pMLKL membrane translocation inhibitor, but not by individual inhibition of caspase or RIPK3. Our data further showed that activation of apoptosis and necroptosis pathways in monocytes differentially contributed to the immune response of monocytes upon H7N9 infection. Specifically, caspase inhibition significantly enhanced, while RIPK3 inhibition reduced the early expression of type I interferons and cytokine/chemokines in H7N9-infected monocytes. Moreover, culture supernatants from IDN-treated H7N9-infected monocyte promoted the expression of co-stimulatory molecule CD80, CD83 and CD86 on freshly isolated monocytes and monocyte-derived dendritic cells (MDCs) and enhanced the capacity of MDCs to induce CD3+ T-cell proliferation in vitro. In contrast, these immune stimulatory effects were abrogated by using culture supernatants from H7N9-infected monocyte with RIPK3 inhibition. In conclusion, our findings indicated that H7N9 infection activated both apoptosis and necroptosis in monocytes. An intact RIPK3 activity is required for upregulation of innate immune responses, while caspase activation suppresses the immune response.
Structural basis for human sterol isomerase in cholesterol biosynthesis and multidrug recognition
Authors: Long Tao , Hassan Abdirahman , Thompson Bonne M , McDonald Jeffrey G , Wang Jiawei , Li Xiaochun 
Published: June 2019

Abstract3-β-hydroxysteroid-Δ8, Δ7-isomerase, known as Emopamil-Binding Protein (EBP), is an endoplasmic reticulum membrane protein involved in cholesterol biosynthesis, autophagy, oligodendrocyte formation. The mutation on EBP can cause Conradi-Hunermann syndrome, an inborn error. Interestingly, EBP binds an abundance of structurally diverse pharmacologically active compounds, causing drug resistance. Here, we report two crystal structures of human EBP, one in complex with the anti-breast cancer drug tamoxifen and the other in complex with the cholesterol biosynthesis inhibitor U18666A. EBP adopts an unreported fold involving five transmembrane-helices (TMs) that creates a membrane cavity presenting a pharmacological binding site that accommodates multiple different ligands. The compounds exploit their positively-charged amine group to mimic the carbocationic sterol intermediate. Mutagenesis studies on specific residues abolish the isomerase activity and decrease the multidrug binding capacity. This work reveals the catalytic mechanism of EBP-mediated isomerization in cholesterol biosynthesis and how this protein may act as a multi-drug binder.
Partitioning surface ligands on nanocrystals for maximal solubility
Authors: Pang Zhenfeng , Zhang Jun , Cao Weicheng , Kong Xueqian , Peng Xiaogang 
Published: June 2019

AbstractA typical colloidal nanoparticle can be viewed as a nanocrystal-ligands complex with an inorganic single-crystalline core, the nanocrystal, bonded with a monolayer of organic ligands. The surface chemistry of nanocrystal-ligands complexes is crucial to their bulk properties. However, deciphering the molecular pictures of the nonperiodic and dynamic organic-inorganic interlayer is a grand technical challenge, and this hampers the quantitative perception of their macroscopic phenomena. Here we show that the atomic arrangement on nanocrystal surface and ligand-ligand interactions can be precisely quantified through comprehensive solid-state nuclear magnetic resonance (SSNMR) methodologies. The analyses reveal that the mixed ligands of n-alkanoates on a CdSe nanocrystal segregate in areal partitions and the unique arrangement unlocks their rotational freedom. The mathematical model based on the NMR-derived ligand partition and dynamics successfully predicts the unusual solubility of nanocrystal-ligands complexes with mixed ligands, which is several orders of magnitude higher than that of nanocrystal-ligands complexes with pure ligands.
Novel Green Biomimetic Approach for Synthesis of ZnO-Ag Nanocomposite; Antimicrobial Activity against Food-borne Pathogen, Biocompatibility and Solar Photocatalysis
Authors: Zare Mina , Namratha Keerthiraj , Alghamdi Saad , Mohammad Yasser Hussein Eissa , Hezam Abdo , Zare Mohamad , Drmosh Qasem Ahmed , Byrappa Kullaiah , Chandrashekar Bananakere Nanjegowda , Ramakrishna Seeram , Zhang Xiang 
Published: June 2019

AbstractA simple, eco-friendly, and biomimetic approach using Thymus vulgaris (T. vulgaris) leaf extract was developed for the formation of ZnO-Ag nanocomposites (NCs) without employing any stabilizer and a chemical surfactant. T. vulgaris leaf extract was used for the first time, in a novel approach, for green fabrication of ZnO-Ag NCs as a size based reducing agent via the hydrothermal method in a single step. Presence of phenols in T. vulgaris leaf extract has served as both reducing and capping agents that play a critical role in the production of ZnO-Ag NCs. The effect of silver nitrate concentration in the formation of ZnO-Ag NCs was studied. The in-vitro Antimicrobial activity of NCs displayed high antimicrobial potency on selective gram negative and positive foodborne pathogens. Antioxidant activity of ZnO-Ag NCs was evaluated via (2,2-diphenyl-1-picrylhydrazyl) DPPH method. Photocatalytic performance of ZnO-Ag NCs was appraised by degradation of phenol under natural sunlight, which exhibited efficient photocatalytic activity on phenol. Cytotoxicity of the NCs was evaluated using the haemolysis assay. Results of this study reveal that T. vulgaris leaf extract, containing phytochemicals, possess reducing property for ZnO-Ag NCs fabrication and the obtained ZnO-Ag NCs could be employed effectively for biological applications in food science. Therefore, the present study offers a promising way to achieve high-efficiency photocatalysis based on the hybrid structure of semiconductor/metal.
Developing regulatory property of gelatin-tannic acid multilayer films for coating-based nitric oxide gas delivery system
Authors: Park Kyungtae , Jeong Hyejoong , Tanum Junjira , Yoo Jae-chan , Hong Jinkee 
Published: June 2019

AbstractTo utilize potentials of nitric oxide (NO) gas in anti-bacterial, anticancer, wound healing applications, numerous studies have been conducted to develop a NO delivery system in the past few decades. Even though a coating method and film types are essential to apply in biomedical device coating from previous NO delivery systems, release control from the coating system is still challenging. In this study, we introduced a multilayered polymeric coating system to overcome the uncontrollable NO release kinetics of film systems. We used biocompatible gelatin and tannic acid to construct a rough, porous structured film based on the layer-by-layer self-assembly method. The multilayered polymeric structure facilitated the controlled amount of NO release from (Gel/TA)n film and showed burst release in early period owing to their large surface area from the rough, porous structure. We synthesized the proton-responsive NO donor, N-diazeniumdiolate (NONOates), into the (Gel/TA)n film through a chemical reaction under high pressure NO gas. NO release profile was analyzed by a real-time NO analysis machine (NOA 280i). Then, the NO-releasing (Gel/TA)n film was tested its toxicity against human dermal fibroblast cells and bactericidal effects against Staphylococcus aureus.
Association of BUD13-ZNF259-APOA5-APOA1-SIK3 cluster polymorphism in 11q23.3 and structure of APOA5 with increased plasma triglyceride levels in a Korean population
Authors: Kim Han-Kyul , Anwar Muhammad Ayaz , Choi Sangdun 
Published: June 2019

AbstractIn this association study on chromosome 11, the data from 12,537 Korean individuals within the Health Examinee (HEXA) and the Korea Association Resource (KARE) projects were analysed to identify genetic loci correlating with increased and decreased plasma triglyceride (TG) levels. We identified a locus in chromosomal region 11q23.3 that harbours genes BUD13, ZNF259, APOA5, APOA1, and SIK3, which may be associated with plasma TG levels. In this locus, 13 relevant single-nucleotide polymorphisms (SNPs) were found: rs184616707, rs118175510, rs60954647, rs79408961, and rs180373 (near BUD13); rs11604424 (in ZNF259); rs2075291, rs651821, and rs7123666 (in or near APOA5); rs525028 (near APOA1), and rs645258, rs10160754, and rs142395187 (in or near SIK3). All 13 SNPs satisfied the genome-wide significance level (P < 5.0 × 10−8) in both meta-analysis and conditional analysis. Haplotype analysis of six SNPs (rs79408961, rs180373, rs2075291, rs651821, rs525028, and rs10160754) that were selected based on the β coefficient and conditional P values, revealed nine common haplotypes (with frequency 0.02–0.34) associated with both increased and reduced TG levels. Furthermore, to shed light on possible structural implications, we modelled and simulated the G185C variant of APOA5 (corresponding to rs2075291), which showed the strongest association. Molecular dynamics simulation results showed that this polymorphic variant of APOA5 has a different hydrogen bond network, increased average distance between chains, and an ability to form distinct clusters. Owing to the orientation of cysteine, the possibility of disulphide bond formation with other proteins is evident. In summary, our association and modelling analyses provided evidence that genetic variations in chromosomal region 11q23.3 are associated with elevated TG levels.
New antimicrobial compounds produced by Seltsamia galinsogisoli sp. nov., isolated from Galinsoga parviflora as potential inhibitors of FtsZ
Authors: Zhang Tian-Yuan , Wu Ying-Ying , Zhang Meng-Yue , Cheng Juan , Dube Blessings , Yu Hui-Jia , Zhang Yi-Xuan 
Published: June 2019

AbstractA total amount of 116 fungal strains, belonging to 30 genera, were acquired from the rhizosphere soil and plant of Galinsoga parviflora. A strain SYPF 7336, isolated from the rhizospheric soil, was identified as Seltsamia galinsogisoli sp. nov., by morphological and molecular analyses, which displayed high antibacterial activity. In order to study the secondary metabolites of Seltsamia galinsogisoli sp. nov., nine compounds were successfully seperated from the strain fermentation broth, including two new compounds and seven known compounds. Their structures were elucidated based on spectral analysis including 1D and 2D NMR. All the seperated compounds were evaluated for their antimicrobial activities. Compounds 2, 5 and 1 displayed antimicrobial activities against Staphylococcus aureus with MIC values of 25, 32 and 75 μg/mL, respectively. Moreover, morphological observation showed the coccoid cells of S. aureus to be swollen to a volume of 1.4 to 1.7-fold after treatment with compounds 1, 2 and 5, respectively. Molecular docking was carried out to investigate interactions of filamentous temperature-sensitive protein Z (FtsZ) with compounds 1, 2 and 5.