bluuebunny/crossref_metadata_2025_split huggingface dataset:
The journey to radiographer advanced practice: a methodological reflection on the use of interpretative phenomenological analysis to explore perceptions and experiences
Authors: Cuthbertson Lynn M. 
Published: August 2019

AbstractIntroduction:This paper is a methodological reflection on the use of interpretative phenomenological analysis (IPA) utilised in the context of a qualitative research project that explored perceptions and experiences of the journey to radiographer advanced practice.Methods and materials:A two-phase qualitative research explored the perceptions and experiences. Phase 1 reviewed reflective diaries (n= 12) kept during the educational phase of the practitioner journeys. Phase 2 included one-to-one, semi-structured interviews (n= 6) which were recorded, transcribed verbatim and reviewed using the IPA six-stage thematic analysis for practitioners embedded in the advanced practice role.Findings:Key themes arising from reflective diary analysis informed the interview content; and following interview transcription, data immersion and IPA, 12 emergent sub-themes generated 3 superordinate themes.Discussion:Theoretical perspectives and application of the methodology are discussed. The phenomenological and interpretative qualities of IPA have the potential to provide unique and valuable insights into lived experiences of individuals. It is hoped that this researchers’ reflections are transferrable for those interested in employing a qualitative methodology for radiotherapy and oncology research.Conclusion:Therapeutic radiographers work within rapidly changing environments from technological, treatment and care perspectives. With continued development and change, the impact of research utilising an IPA methodology may allow exploration of perceptions and experiences from a range of key stakeholders with the potential to increase the research base.
Interference of turnipweed (Rapistrum rugosum) and Mexican pricklepoppy (Argemone mexicana) in wheat
Authors: Manalil Sudheesh , Chauhan Bhagirath Singh 
Published: August 2019

AbstractTurnipweed [Rapistrum rugosum (L.) All.] and Mexican pricklepoppy (Argemone mexicana L.) are increasingly prevalent in the northern cropping regions of Australia. The effect of different densities of these two weeds was examined for their potential to cause yield loss in wheat (Triticum aestivum L.) through field studies in 2016 and 2017. There was 72% to 78% yield reduction in wheat due to competition from R. rugosum. Based on the exponential decay model, 18.2 and 24.3 plants m−2 caused a yield reduction of 50% in 2016 and 2017, respectively. Rapistrum rugosum produced a maximum of 32,042 and 29,761 seeds m−2 in 2016 and 2017, respectively. There was 100% weed seed retention at crop harvest. Competition from A. mexicana resulted in a yield loss of 17% and 22% in 2016 and 2017, respectively; however, plants failed to set seeds due to intense competition from wheat. Among the yield components, panicles per square meter and grains per panicle were affected by weed competition. The studies indicate a superior competitiveness of R. rugosum in wheat and a suppressive effect of wheat on A. mexicana. The results indicate that a wheat crop can be included in crop rotation programs where crop fields are infested with A. mexicana. High seed retention in R. rugosum indicates the possibility to manage this weed through seed catching and harvest weed seed destruction.
Targeted exon skipping with AAV-mediated split adenine base editors
Authors: Winter Jackson , Luu Alan , Gapinske Michael , Manandhar Sony , Shirguppe Shraddha , Woods Wendy S. , Song Jun S. , Perez-Pinera Pablo 
Published: August 2019

AbstractTechniques for exclusion of exons from mature transcripts have been applied as gene therapies for treating many different diseases. Since exon skipping has been traditionally accomplished using technologies that have a transient effect, it is particularly important to develop new techniques that enable permanent exon skipping. We have recently shown that this can be accomplished using cytidine base editors for permanently disabling the splice acceptor of target exons. We now demonstrate the application of CRISPR-Cas9 adenine deaminase base editors to disrupt the conserved adenine within splice acceptor sites for programmable exon skipping. We also demonstrate that by altering the amino acid sequence of the linker between the adenosine deaminase domain and the Cas9-nickase or by coupling the adenine base editor with a uracil glycosylase inhibitor, the DNA editing efficiency and exon-skipping rates improve significantly. Finally, we developed a split base editor architecture compatible with adeno-associated viral packaging. Collectively, these results represent significant progress toward permanent in vivo exon skipping through base editing and, ultimately, a new modality of gene therapy for the treatment of genetic diseases.
A metabolic profile of all-cause mortality risk identified in an observational study of 44,168 individuals
Authors: Deelen Joris , Kettunen Johannes , Fischer Krista , van der Spek Ashley , Trompet Stella , Kastenmüller Gabi , Boyd Andy , Zierer Jonas , van den Akker Erik B. , Ala-Korpela Mika , Amin Najaf , Demirkan Ayse , Ghanbari Mohsen , van Heemst Diana , Ikram M. Arfan , van Klinken Jan Bert , Mooijaart Simon P. , Peters Annette , Salomaa Veikko , Sattar Naveed , Spector Tim D. , Tiemeier Henning , Verhoeven Aswin , Waldenberger Melanie , Würtz Peter , Davey Smith George , Metspalu Andres , Perola Markus , Menni Cristina , Geleijnse Johanna M. , Drenos Fotios , Beekman Marian , Jukema J. Wouter , van Duijn Cornelia M. , Slagboom P. Eline 
Published: August 2019

AbstractPredicting longer-term mortality risk requires collection of clinical data, which is often cumbersome. Therefore, we use a well-standardized metabolomics platform to identify metabolic predictors of long-term mortality in the circulation of 44,168 individuals (age at baseline 18–109), of whom 5512 died during follow-up. We apply a stepwise (forward-backward) procedure based on meta-analysis results and identify 14 circulating biomarkers independently associating with all-cause mortality. Overall, these associations are similar in men and women and across different age strata. We subsequently show that the prediction accuracy of 5- and 10-year mortality based on a model containing the identified biomarkers and sex (C-statistic = 0.837 and 0.830, respectively) is better than that of a model containing conventional risk factors for mortality (C-statistic = 0.772 and 0.790, respectively). The use of the identified metabolic profile as a predictor of mortality or surrogate endpoint in clinical studies needs further investigation.
Air pollution exposure associates with increased risk of neonatal jaundice
Authors: Zhang Liqiang , Liu Weiwei , Hou Kun , Lin Jintai , Song Changqing , Zhou Chenghu , Huang Bo , Tong Xiaohua , Wang Jinfeng , Rhine William , Jiao Ying , Wang Ziwei , Ni Ruijing , Liu Mengyao , Zhang Liang , Wang Ziye , Wang Yuebin , Li Xingang , Liu Suhong , Wang Yanhong 
Published: August 2019

AbstractClinical experience suggests increased incidences of neonatal jaundice when air quality worsens, yet no studies have quantified this relationship. Here we reports investigations in 25,782 newborns showing an increase in newborn’s bilirubin levels, the indicator of neonatal jaundice risk, by 0.076 (95% CI: 0.027–0.125), 0.029 (0.014–0.044) and 0.009 (95% CI: 0.002–0.016) mg/dL per μg/m3 for PM2.5 exposure in the concentration ranges of 10–35, 35–75 and 75–200 μg/m3, respectively. The response is 0.094 (0.077–0.111) and 0.161 (0.07–0.252) mg/dL per μg/m3 for SO2 exposure at 10–15 and above 15 μg/m3, respectively, and 0.351 (0.314–0.388) mg/dL per mg/m3 for CO exposure. Bilirubin levels increase linearly with exposure time between 0 and 48 h. Positive relationship between maternal exposure and newborn bilirubin level is also quantitated. The jaundice−pollution relationship is not affected by top-of-atmosphere incident solar irradiance and atmospheric visibility. Improving air quality may therefore be key to lowering the neonatal jaundice risk.
Probing molecular mechanisms of M13 bacteriophage adhesion
Authors: Lim Chanoong , Ko Jina , Jeon Dasom , Song Yoojung , Park Jinwoo , Ryu Jungki , Lee Dong Woog 
Published: August 2019

AbstractM13 bacteriophages can provide a versatile platform for nanobiotechnology because of their unique biological and physicochemical properties. Polypeptides on their surfaces can be finely tuned on demand through genetic engineering, enabling tailored assembly of multiple functional components through specific interactions. Their versatility has been demonstrated by synthesizing various unprecedented hybrid materials for energy storage, biosensing, and catalysis. Here we select a specific type of genetically engineered M13 bacteriophage (DSPH) to investigate the origin of interactions. The interaction forces between the phage-coated surface and five different functionalized self-assembled monolayers are directly measured using a surface forces apparatus. We confirm that the phages have strong adhesion energies in acidic environments due to π-π stacking and hydrophobic interactions, while hydrogen bonding interactions remain relatively weak. These results provide quantitative and qualitative information of the molecular interaction mechanisms of DSPH phages, which can be utilized as a database of the bacteriophage interactions.
Determining Emotional Tone and Verbal Behavior in Patients With Tinnitus and Hyperacusis: An Exploratory Mixed-Methods Study
Authors: Kimball Suzanne H. , Hamilton Toby , Benear Erin , Baldwin Jonathan 
Published: August 2019

PurposeThe purpose of this study was to evaluate the emotional tone and verbal behavior of social media users who self-identified as having tinnitus and/or hyperacusis that caused self-described negative consequences on daily life or health.Research Design and MethodAn explanatory mixed-methods design was utilized. Two hundred “initial” and 200 “reply” Facebook posts were collected from members of a tinnitus group and a hyperacusis group. Data were analyzed via the LIWC 2015 software program and compared to typical bloggers. As this was an explanatory mixed-methods study, we used qualitative thematic analyses to explain, interpret, and illustrate the quantitative results.ResultsOverall, quantitative results indicated lower overall emotional tone for all categories (tinnitus and hyperacusis, initial and reply), which was mostly influenced by higher negative emotion. Higher levels of authenticity or truth were found in the hyperacusis sample but not in the tinnitus sample. Lower levels of clout (social standing) were indicated in all groups, and a lower level of analytical thinking style (concepts and complex categories rather than narratives) was found in the hyperacusis sample. Additional analysis of the language indicated higher levels of sadness and anxiety in all groups and lower levels of anger, particularly for initial replies. These data support prior findings indicating higher levels of anxiety and depression in this patient population based on the actual words in blog posts and not from self-report questionnaires. Qualitative results identified 3 major themes from both the tinnitus and hyperacusis texts: suffering, negative emotional tone, and coping strategies.ConclusionsResults from this study suggest support for the predominant clinical view that patients with tinnitus and hyperacusis have higher levels of anxiety and depression than the general population. The extent of the suffering described and patterns of coping strategies suggest clinical practice patterns and the need for research in implementing improved practice plans.
Effects of Grade and School Services on Children's Responsibility for Hearing Aid Care
Authors: Klein Kelsey E. , Spratford Meredith , Redfern Alexandra , Walker Elizabeth A. 
Published: August 2019

Purpose
We investigated trends in hearing aid maintenance and assumption of responsibility for hearing aids in school-age children who are hard of hearing. Specifically, we examined the extent to which families own necessary hearing aid maintenance equipment, whether and by whom hearing aid maintenance tasks are being completed, and the effects of grade and receipt of an Individualized Education Program (IEP) or 504 plan on a child's assumption of responsibility for hearing aid care.

Method
Participants included 167 children who are hard of hearing in 1st to 4th grade. Caregivers reported whether the families owned various types of hearing aid maintenance equipment (listening tube, battery tester, and dri-aid kit) and who normally completes various hearing aid maintenance tasks. Information about children's audiological characteristics was also collected.

Results
Thirty-two percent of families reported not owning at least 1 piece of hearing aid maintenance equipment. Using a battery tester and performing a listening check were the maintenance tasks completed the least frequently, with 49% and 28% of caregivers reporting that these tasks are not completed regularly, respectively. Children's responsibility for hearing aid maintenance increased with grade. After controlling for maternal education and degree of hearing loss, children with an IEP or 504 plan took more responsibility for hearing aid maintenance tasks than children without these services.

Conclusion
Important hearing aid maintenance tasks, such as listening checks, are not completed regularly for many children, even when families own the necessary equipment. Ensuring that children who are hard of hearing have an IEP or 504 plan throughout elementary school may support self-advocacy and encourage children to take responsibility for their hearing aids, which may lead to more consistent hearing aid functioning.
Optimization of biogas composition in experimental studies
Authors: Dimitrov Radostin , Ivanov Zdravko , Zlateva Penka , Mihaylov Veselin 
Published: August 2019

The article is focused on the potential and application of biogas, as an alternative fuel from Renewable Energy Sources, for use mainly in gas-generator stations. Biogas fuel is basically a mixture of methane and carbon dioxide. Its composition depends on the type of raw material used for its production. Methane concentration in biogas is between 50÷80%. To be possible engine to work with maximum efficiency with different biogas fuels, it is necessary to modify specific adjustment parameters depending on the concentration of methane in the mixture. This requires the creation of a biogas simulation system for different concentrations of the main components.
The aim is to investigate and determine the optimum and permissible biofuel blend concentrations and their impact on engine performance and fuel consumption. Biogas can be used as a fuel to produce electricity, heat or steam or as fuel for internal combustion engine, and its use will help to reduce harmful emissions into the atmosphere.
Addressing Decision Making in Progesterone Treatment for History of Preterm Delivery
Authors: Zuponcic Jacqueline , Cottrell Connie , Lavin Justin , Facchini Wendy , Li Marissa 
Published: August 2019

Introduction The United States ranks 27th among nations worldwide for infant mortality with a rate of 6.1 deaths per 1,000 live births. The majority of perinatal morbidity and mortality is related to preterm birth, defined as delivery prior to 37 weeks' gestation. Among the risk factors for preterm birth is prior preterm birth, which is associated with a 1.5- to 2.0-fold increase in risk. At the present time, there is only one Food and Drug Administration approved treatment for the prevention of preterm birth among women with a history of prior spontaneous premature delivery, intramuscular 17-α-hydroxyprogesterone caproate (17-OHP), administered once weekly from 20 to 36 weeks' gestation. However, many eligible pregnant patients decline this therapy.
Methods This was a prospective, cohort study involving patients who were identified as candidates for 17-OHP treatment at their first obstetric visit and asked to complete a short survey regarding their history of preterm birth. Those patients who consented to a follow-up phone call were asked to participate in a focus group discussion regarding their experience with progesterone and the health care system.
Results During the 1-year study period, 55 progesterone candidates were identified, 43 accepted treatment, 7 refused, and 5 either initiated prenatal care too late to receive injections or did not follow-up. Those who accepted treatment appeared to cope better with treatment side effects, and/or had traumatic emotional reactions regarding their prior premature birth outcomes. Women who declined treatment often cited pain with injection, had fatalistic beliefs regarding their care, and/or had personal concerns related to full-term pregnancy.
Discussion Maternal health care providers should always discuss the implications of prematurity at the time of the index premature delivery and again at the first prenatal visit of the subsequent pregnancy. Providers need to be prepared to employ various techniques for patient counseling and education. Small changes in office practice, like having fewer care providers involved in patient care or providing distractions for children, may make the difference between a patient who is open or closed to treatment options.
Animal Models of Hirschsprung's Disease: State of the Art in Translating Experimental Research to the Bedside
Authors: Fujiwara Naho , Nakazawa-Tanaka Nana , Yamataka Atsuyuki 
Published: August 2019

AbstractHirschsprung's disease (HSCR) is caused by incomplete colonization of enteric neural crest-derived cell (ENCC) in the bowel, the failure of ENCCs to proliferate, differentiate, and migrate leads to an absence of enteric neurons in the distal colon, resulting in colonic motility dysfunction. Various animal models of HSCR have been important in the understanding of the anatomy and pathophysiology of the disease and in the discovery of genes involved in HSCR. Four types of HSCR animal models have been developed: teratogen-induced, surgically created, naturally occurring models, and knockout models. Mutations in several genes affect enteric nervous system (ENS) development and can have pleiotropic effects on this system. Furthermore, certain animal models are informative regarding how such molecules control the development and functional differentiation of the ENS. In this article, we summarize recent advances in this field and highlight opportunities for new discoveries.
Thrombosis Risk in Cancer Patients Receiving Red Blood Cell Transfusions
Authors: Ramsey Glenn , Lindholm Paul F. 
Published: August 2019

AbstractPatients with cancer have increased risk of thrombosis and often need red blood cell (RBC) transfusions. However, RBC transfusions may also promote thrombosis because of raised hematocrit and viscosity, storage-related RBC damage, and exposure to thrombogenic mediators from obsolescent RBCs. The authors conducted a literature survey for studies examining whether RBC transfusions were associated with increased risk of venous thromboembolism (VTE) in cancer patients. In perioperative cancer surgery patients with categorical comparisons of any versus no RBC transfusion, increased risk of VTE with RBC transfusion was found in 11 of 31 studies, 5 by univariate correlation only and 6 in multivariate analysis. All six multivariate-positive studies had intermediate overall rates of thrombosis (1.4–6.0%), and three were in urological surgery series. In the larger studies of > 2,000 patients (range: 2,219–44,656), the maximum odds ratio among the multivariate-positive studies was 1.3. Perioperative RBC transfusion volume was more strongly associated with VTE risk, with a positive association in six of seven studies. One large registry-based study of hospitalized cancer patients, not restricted to the perioperative setting, found an adjusted odds ratio of 1.60 (95% confidence interval: 1.53–1.67) for VTE risk in patients receiving RBCs compared with nontransfused patients.
Population-Based Estimation of Dental Caries and Periodontal Disease Rates of Gravid and Recently Postpartum Women in Lilongwe, Malawi
Authors: Antony Kathleen M. , Kazembe Peter N. , Pace Ryan M. , Levison Judy , Mlotha-Namarika Jesse , Phiri Henry , Chiudzu Grace , Harris R. Alan , Aagaard Joshua , Twyman Nicholas , Ramin Susan M. , Raine Susan P. , Belfort Michael A. , Aagaard Kjersti M. 
Published: August 2019

Objectives The objective of this study was to determine the rate of dental caries and periodontal disease among gravid and recently postpartum women at five delivery centers within and surrounding Lilongwe, Malawi.
Study Design We partnered with obstetric specialists, community health workers, and dentists to perform dental history interviews and dental examinations during the study period from December 2012 to May 2014. Dental examinations were performed according to World Health Organization standards to assess periodontal and oral health status.
Results Among the 387 gravid and recently postpartum women, the rate of dental caries was 69.3% and the rate of composite dental disease (caries and periodontal disease) was 76.7%. The majority (69.5%) of women examined had a decayed-missing-filled (DMF) index greater than or equal to one; the average DMF Index was 2.48. The majority of women had never seen a dentist (62.8%). However, most did perform oral hygiene, two or more times per day (90.2%); most women reported brushing with toothpaste (88.1%).
Conclusion When assessing this population for dental caries and periodontal disease, the rate of dental disease was high. Therefore, this may be an ideal setting to test for impactful interventions aimed at reducing caries and periodontal disease.
TNFR2 promotes Treg-mediated recovery from neuropathic pain across sexes
Authors: Fischer Roman , Sendetski Maksim , del Rivero Tania , Martinez George F. , Bracchi-Ricard Valerie , Swanson Kathryn A. , Pruzinsky Elizabeth K. , Delguercio Niky , Rosalino Michael J. , Padutsch Tanja , Kontermann Roland E. , Pfizenmaier Klaus , Bethea John R. 
Published: August 2019

Significance
Tumor necrosis factor (TNF) is a cytokine that induces signaling via two receptors, TNFR1 and TNFR2. TNF signaling via TNFR1 contributes to development and maintenance of neuropathic pain. Here, we show that TNFR2 is essential for recovery from neuropathic pain across sexes. Treatment of male and female neuropathic mice with a TNFR2 agonist resulted in long-lasting recovery from neuropathic pain. We identified Tregs as the cellular mediator of the analgesic effect of TNFR2. Indeed, TNFR2 agonist administration alleviated peripheral and central inflammation and promoted neuroprotection in a Treg-dependent manner, indicating that TNFR2-dependent modulation of immunity is neuroprotective. We therefore argue that TNFR2 agonists might be a class of nonopioid drugs that can promote long-lasting pain recovery in males and females.
Awakening: Predicting external stimulation to force transitions between different brain states
Authors: Deco Gustavo , Cruzat Josephine , Cabral Joana , Tagliazucchi Enzo , Laufs Helmut , Logothetis Nikos K. , Kringelbach Morten L. 
Published: August 2019

Significance
We describe a quantitative and robust definition of a brain state as an ensemble of “metastable substates,” each with a probabilistic stability and occurrence frequency. Fitting this to a generative whole-brain model provides an innovative avenue for predicting where simulated brain stimulation can force transitions between different brain states. We provide proof-of-concept by systematically applying this model framework to neuroimaging data of the human sleep cycle and show where to stimulate to awaken the human sleeping brain and vice versa. These results suggest an avenue for using causal whole-brain models to discover in silico where to force a transition between brain states, which may potentially support recovery in disease.