bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cross-decoding reveals shared brain activity patterns between saccadic eye-movements and semantic processing of implicitly spatial words
Authors: Ostarek Markus , Paridon Jeroen van , Huettig Falk 
Published: September 2018

AbstractProcessing words with referents that are typically observed up or down in space (up/down words) influences the subsequent identification of visual targets in congruent locations. Eye-tracking studies have shown that up/down word comprehension shortens launch times of subsequent saccades to congruent locations and modulates concurrent saccade trajectories. This can be explained by a task-dependent interaction of semantic processing and oculomotor programs or by a direct recruitment of direction-specific processes in oculomotor and spatial systems as part of semantic processing. To test the latter possibility, we conducted a functional magnetic resonance imaging experiment and used multi-voxel pattern analysis to assess 1) whether the typical location of word referents can be decoded from the fronto-parietal spatial network and 2) whether activity patterns are shared between up/down words and up/down saccadic eye movements. In line with these hypotheses, significant decoding of up vs. down words and cross-decoding between up/down saccades and up/down words were observed in the frontal eye field region in the superior frontal sulcus and the inferior parietal lobule. Beyond these spatial attention areas, typical location of word referents could be decoded from a set of occipital, temporal, and frontal areas, indicating that interactions between high-level regions typically implicated with lexical-semantic processing and spatial/oculomotor regions constitute the neural basis for access to spatial aspects of word meanings.
A synthetic biology platform for the reconstitution and mechanistic dissection of LINC complex assembly
Authors: Majumder Sagardip , Willey Patrick T. , DeNies Maxwell S. , Liu Allen P. , Luxton G.W. Gant 
Published: September 2018

ABSTRACTThe linker of nucleoskeleton and cytoskeleton (LINC) is a conserved nuclear envelope-spanning molecular bridge that is responsible for the mechanical integration of the nucleus with the cytoskeleton. LINC complexes are formed by a transluminal interaction between the outer and inner nuclear membrane KASH and SUN proteins, respectively. Despite recent structural insights, our mechanistic understanding of LINC complex assembly remains limited by the lack of an experimental system for its in vitro reconstitution and manipulation. Here, we describe artificial nuclear membranes (ANMs) as a synthetic biology platform based on mammalian cell-free expression for the rapid reconstitution of SUN proteins in supported lipid bilayers. We demonstrate that SUN1 and SUN2 are oriented in ANMs with solvent-exposed C-terminal KASH-binding SUN domains. We also find that SUN2 possesses a single transmembrane domain, while SUN1 possesses three. Finally, SUN protein-containing ANMs bind synthetic KASH peptides, thereby reconstituting the LINC complex core. This work represents the first in vitro reconstitution of KASH-binding SUN proteins in supported lipid bilayers using cell-free expression, which will be invaluable for testing proposed models of LINC complex assembly and its regulation.
The Plant PTM Viewer, a central resource exploring plant protein modifications. From site-seeing to protein function
Authors: Willems Patrick , Horne Alison , Goormachtig Sofie , Smet Ive De , Botzki Alexander , Breusegem Frank Van , Gevaert Kris 
Published: September 2018

SUMMARYPosttranslational modifications (PTMs) of proteins are central in any kind of cellular signaling. Modern mass spectrometry technologies enable comprehensive identification and quantification of various PTMs. Given the increased number and types of mapped protein modifications, a database is necessary that simultaneouly integrates and compares site-specific information for different PTMs, especially in plants for which the available PTM data are poorly catalogued. Here, we present the Plant PTM Viewer (http://www.psb.ugent.be/PlantPTMViewer), an integrative PTM resource that comprises approximately 200,000 PTM sites for 17 types of protein modifications in plant proteins from five different species. The Plant PTM Viewer provides the user with a protein sequence overview in which the experimentally evidenced PTMs are highlighted together with functional protein domains or active site residues. The PTM sequence search tool can query PTM combinations in specific protein sequences, whereas the PTM BLAST tool searches for modified protein sequences to detect conserved PTMs in homologous sequences. Taken together, these tools facilitate to assume the role and potential interplay of PTMs in specific proteins or within a broader systems biology context. The Plant PTM Viewer is an open repository that allows submission of mass spectrometry-based PTM data to remain at pace with future PTM plant studies.
Laboratory surrogate markers of residual HIV replication among distinct groups of individuals under antiretroviral therapy
Authors: Giron L.B. , Tenore S.B. , Janini L.M. , Sucupira M.C. , Diaz R.S. 
Published: September 2018

AbstractBackgroundResidual HIV-1 replication among individuals under antiretroviral therapy (ART) relates to HIV micro-inflammation.ObjectivesTo determine levels of residual HIV replication markers among distinct subgroups of antiretroviral-treated individuals.Methods116 patients were distributed into 5 treatment groups: first-line suppressive ART with non-nucleoside reverse-transcriptase inhibitor (NNRTI) (n=26), first-line suppressive ART with boosted protease inhibitors (PI-r) (n=25), salvage therapy using PI-r (n=27), salvage therapy with PI-r and raltegravir (n=22) and virologic failure (n=16). Episomal and total DNA quantitation was evaluated. ELISA was used for HIV antibody and LPS quantitation.ResultsEpisomal DNA was positive in 26% to 38% of individuals under suppressive ART, being higher among individuals experiencing virologic failure (p=0.04). HIV proviral load was higher among patients with detectable episomal DNA (p=0.01). Individuals receiving initial PI-r treatment presented lower HIV antibodies (p=0.027) and LPS (p=0.029) than individuals receiving NNRTI. There was a negative correlation between episomal DNA quantitation and suppressive ART duration (p=0.04), CD4+ T-cell count (p=0.08), and CD8+ T-cell count (p=0.07).ConclusionsResidual HIV replication has been inferred among individuals under suppressive ART according to episomal DNA detection. Residual replication may decrease with longer periods of suppressive ART and higher levels of CD4+ and CD8+ T cells. The relationship between episomal DNA and total DNA suggests a replenishment of the proviral reservoir with impacts on HIV persistence. Lower antibody and LPS levels among patients with initial PI-r ART suggest these regimens may more effectively suppress HIV with higher capacity to decrease the HIV antigenic component.
Endothelial cells form transient Notch-dependent NO-containing cystic structures during zebrafish cerebrovascular development
Authors: Kugler E. , Chhabria K. , Daetwyler S. , Huisken J. , Plant K. , Savage A.M. , Wilkinson R.N. , Armitage P.A. , Chico T.J.A. 
Published: September 2018

AbstractEndothelial cell behaviour during blood vessel formation is highly complex and dynamic. Transgenic zebrafish have provided many new insights into these processes, due to their ability to provide detailed in vivo imaging.We here report a previously undescribed endothelial cell behaviour during zebrafish embryonic development. Endothelial cells of the cerebral vessels of 3-5d post fertilisation embryos extruded large membranous spherical structures. These were only found on the cerebral vessels, and did not detach from the parent vessel, instead regressing back into the endothelial cell. These structures did not communicate with the vessel lumen, exhibited periodic oscillations in size and shape, and were enriched with filamentous actin at their neck. Due to their unknown nature and spherical appearance we termed these structures kugeln (German for sphere).Pharmacological inhibition of vascular endothelial growth factor (VEGF) signalling significantly increased kugel number while Notch inhibition significantly reduced both kugel number and diameter. Kugeln contain little cytoplasm, but are highly positive for nitric oxide (NO) reactivity, suggesting they represent a novel NO containing organelle specific to the cerebral vessels.
In vivo assay of cortical microcircuitry in frontotemporal dementia: a platform for experimental medicine studies
Authors: Shaw Alexander D , Hughes Laura E , Moran Rosalyn , Coyle-Gilchrist Ian , Rittman Tim , Rowe James B 
Published: September 2018

AbstractThe analysis of neural circuits can provide critical insights into the mechanisms of neurodegeneration and dementias, and offer potential quantitative biological tools to assess novel therapeutics. Here we use behavioural variant frontotemporal dementia (bvFTD) as a model disease. We demonstrate that inversion of canonical microcircuit models to non-invasive human magnetoecphalography can identify the regional- and laminar-specificity of bvFTD pathophysiology, and their parameters can accurately differentiate patients from matched healthy controls. Using such models, we show that changes in local coupling in frontotemporal dementia underlie the failure to adequately establish sensory predictions, leading to altered prediction error responses in a cortical information-processing hierarchy. Using machine learning, this model-based approach provided greater case-control classification accuracy than conventional evoked cortical responses. We suggest that this approach provides an in vivo platform for testing mechanistic hypotheses about disease progression and pharmacotherapeutics.
A transgenic mutant mouse line accompanied by the complete deletion of interleukin-33 showed insulin and leptin resistances
Authors: Watanabe Taku , Takeuchi Tomonori , Kubota Naoto , Wainai Tasuku , Kataoka Keisuke , Nakaya Toshitaka , Sugimoto Ayako , Sato Takahiro , Ohira Hiroshi , Tsujino Ichizo , Kumagai Katsuyoshi , Kubota Tetsuya , Hasegawa Chiaki , Tokuyama Kumpei , Ueki Kohjiro , Yamauchi Toshimasa , Mishina Masayoshi , Kadowaki Takashi 
Published: September 2018

ABSTRACTInterleukin (IL) −33 has been identified as a member of the IL-1 family. Members of the IL-1 family have been reported to be involved in the regulation of energy homeostasis and glucose metabolism. Homozygous transgenic mutant mice of FLP14 line, that we previously generated, unexpectedly developed mature-onset obesity and diabetes. Through genetic investigations, we found that insertion of the transgenes had resulted in complete deletion of the Il33 gene. These obese male homozygous mutant mice exhibited hyperphagia with hyperleptinemia and insulin resistance caused by increased hepatic gluconeogenesis and decreased glucose uptake in skeletal muscle. As a result of examining preobese male homozygous mutant mice to investigate with the exclusion of the effect of obesity, hyperphagia with hyperleptinemina and insulin resistance caused by decreased glucose uptake in skeletal muscle were already observed, but the increased hepatic glucose production was not. To investigate whether the insulin resistance was caused by deletion of the Il33 gene, we treated these preobese homozygous mutant mice with recombinant IL-33 protein and noted a significant improvement in insulin resistance. Thus, insulin resistance in these homozygous mutant mice was caused, at least in part, by IL-33 deficiency, suggesting a favorable role of IL-33 for glucose metabolism in the skeletal muscle.
The landscape of somatic mutation in normal colorectal epithelial cells
Authors: Lee-Six Henry , Ellis Peter , Osborne Robert J. , Sanders Mathijs A. , Moore Luiza , Georgakopoulos Nikitas , Torrente Franco , Noorani Ayesha , Goddard Martin , Robinson Philip , Coorens Tim H. H. , O’Neill Laura , Alder Christopher , Wang Jingwei , Fitzgerald Rebecca C. , Zilbauer Matthias , Coleman Nicholas , Saeb-Parsy Kourosh , Martincorena Inigo , Campbell Peter J. , Stratton Michael R. 
Published: September 2018

AbstractThe colorectal adenoma-carcinoma sequence has provided a paradigmatic framework for understanding the successive somatic genetic changes and consequent clonal expansions leading to cancer. As for most cancer types, however, understanding of the earliest phases of colorectal neoplastic change, which may occur in morphologically normal tissue, is comparatively limited because of the difficulty of detecting somatic mutations in normal cells. Each colorectal crypt is a small clone of cells derived from a single recently-existing stem cell. Here, we whole genome sequenced hundreds of normal crypts from 42 individuals. Signatures of multiple mutational processes were revealed, some ubiquitous and continuous, others only found in some individuals, in some crypts or during some phases of the cell lineage from zygote to adult cell. Likely driver mutations were present in ∼1% of normal colorectal crypts in middle-aged individuals, indicating that adenomas and carcinomas are rare outcomes of a pervasive process of neoplastic change across morphologically normal colorectal epithelium.
The influence of human and relational capital on the rapid internationalization of firms
Authors: Baier-Fuentes Hugo , Hormiga Esther , Amorós José Ernesto , Urbano David 
Published: September 2018

PurposeThe purpose of this paper is to compare the influence of entrepreneurs’ human and relational capital on the rapid internationalization of their firms from two economically different contexts.Design/methodology/approachThis study was developed using data that were collected from the Global Entrepreneurship Monitor of Spain and Chile. A logistic regression analysis was used to examine and compare the influence of some elements of entrepreneurs’ human and relational capital on the likelihood of their firms’ rapid internationalization.FindingsThe results revealed that Chilean entrepreneurs rely more on their formal education or experience to rapidly internationalize their firms. In contrast, Spanish entrepreneurs complement their formal education with their relational capital to conduct international entrepreneurial activities.Research limitations/implicationsThe implications of this study are related to the role that public policies play in promoting these types of entrepreneurial initiatives.Originality/valueThis study presents several contributions. First, it advances the understanding of entrepreneurial internationalization in emerging economic contexts. Second, it provides a comparative study regarding entrepreneurial internationalization, which is considered a fundamental current in the field of international entrepreneurship. Finally, this comparative study improves our understanding of the influence of different economic contexts on entrepreneurial internationalization.
How customer orientation leads to customer satisfaction
Authors: Tseng Lu-Ming 
Published: September 2018

PurposeFinancial service workers’ etiquette and creativity have been less mentioned in the research on bank marketing. The purpose of this paper is to examine the mediating effects of financial service workers’ etiquette and creativity on the relationship between customer orientation and customer satisfaction.Design/methodology/approachData are collected from 336 insurance customers in Taiwan, and partial least squares analysis is used to test the hypotheses.FindingsThe findings show that financial service workers’ customer orientation (perceived by customers) is positively related to the customers’ perception of financial service workers’ etiquette and creativity. Moreover, it is found that the financial service workers’ etiquette and creativity partially mediate the relationship between customer orientation and customer satisfaction.Originality/valueThis is the first study to highlight the roles that financial service workers’ etiquette and creativity play in the relationship between customer orientation and customer satisfaction.
Improved energy conversion efficiency of the ultrasonic motor with surface texture
Authors: Liu Xiaoliang , Qiu Jinhao , Zhao Gai 
Published: September 2018

Purpose
This paper aims to investigate the effect of frictional materials and surface texture on the energy conversion efficiency and the mechanical output performance of the ultrasonic motor (USM).

Design/methodology/approach
A newly designed testing system was set up to measure the mechanical output performance of the USM. The influence of different frictional materials on the output performance of the USM was studied under the same assembly process and parameters. The surface texture was fabricated by laser ablation processing. The effects of surface texture and input parameters on the energy conversion efficiency and mechanical output performance of the USM were studied.

Findings
The results show that polyimide (PI) composites as frictional material can significantly improve the output performance of the USM compared to polytetrafluoroethylene (PTFE) composites. When the pre-load is 240 N, the energy conversion efficiency of the USM using textured PI composites as frictional material can reach 41.93 per cent, increased by 29.21 per cent compared to PTFE composites, and the effective output range of the USM is increased to 0.7-1.1 N m. Besides, the pre-load and surface texture have a great influence on the output performance of the USM.

Originality/value
PI composites can improve the mechanical output performance of the USM. Surface texture can also improve the interface tribological properties and the energy conversion efficiency based on the advanced frictional materials, which will contribute to the increment of the output performance of the USM under the same input conditions.
Hybrid flow shop with multiprocessor task scheduling based on earliness and tardiness penalties
Authors: Engin Orhan , Engin Batuhan 
Published: September 2018

Purpose
Hybrid flow shop with multiprocessor task (HFSMT) has received considerable attention in recent years. The purpose of this paper is to consider an HFSMT scheduling under the environment of a common time window. The window size and location are considered to be given parameters. The research deals with the criterion of total penalty cost minimization incurred by earliness and tardiness of jobs. In this research, a new memetic algorithm in which a global search algorithm is accompanied with the local search mechanism is developed to solve the HFSMT with jobs having a common time window. The operating parameters of memetic algorithm have an important role on the quality of solution. In this paper, a full factorial experimental design is used to determining the best parameters of memetic algorithm for each problem type. Memetic algorithm is tested using HFSMT problems.

Design/methodology/approach
First, hybrid flow shop scheduling system and hybrid flow shop scheduling with multiprocessor task are defined. The applications of the hybrid flow shop system are explained. Also the background of hybrid flow shop with multiprocessor is given in the introduction. The features of the proposed memetic algorithm are described in Section 2. The experiment results are presented in Section 3.

Findings
Computational experiments show that the proposed new memetic algorithm is an effective and efficient approach for solving the HFSMT under the environment of a common time window.

Originality/value
There is only one study about HFSMT scheduling with time window. This is the first study which added the windows to the jobs in HFSMT problems.
Image search and retrieval problems in web search engines
Authors: Norouzi Yaghoub , Homavandi Hoda 
Published: September 2018

PurposeThe purpose of this paper is to investigate image search and retrieval problems in selected search engines in relation to Persian writing style challenges.Design/methodology/approachThis study is an applied one, and to answer the questions the authors used an evaluative research method. The aim of the research is to explore the morphological and semantic problems of Persian language in connection with image search and retrieval among the three major and widespread search engines: Google, Yahoo and Bing. In order to collect the data, a checklist designed by the researcher was used and then the data were analyzed by descriptive and inferential statistics.FindingsThe results indicate that Google, Yahoo and Bing search engines do not pay enough attention to morphological and semantic features of Persian language in image search and retrieval. This research reveals that six groups of Persian language features include derived words, derived/compound words, Persian and Arabic Plural words, use of dotted T and the use of spoken language and polysemy, which are the major problems in this area. In addition, the results suggest that Google is the best search engine of all in terms of compatibility with Persian language features.Originality/valueThis study investigated some new aspects of the above-mentioned subject through combining morphological and semantic aspects of Persian language with image search and retrieval. Therefore, this study is an interdisciplinary research, the results of which would help both to offer some solutions and to carry out similar research on this subject area. This study will also fill a gap in research studies conducted so far in this area in Farsi language, especially in image search and retrieval. Moreover, findings of this study can help to bridge the gap between the user’s questions and search engines (systems) retrievals. In addition, the methodology of this paper provides a framework for further research on image search and retrieval in databases and search engines.