bluuebunny/crossref_metadata_2025_split huggingface dataset:
OPTIMIZATION OF RED PIGMENT PRODUCTION BY MONASCUS PURPUREUS FTC 5356 USING RESPONSE SURFACE METHODOLOGY
Authors: M. Said Farhan , Hamid Nor Farhana Binti 
Published: June 2018

Factors such as environmental conditions and nutrients are significant for successful growth and reproduction of microorganisms. Manipulations of the factors are the most effective way to stimulate the growth of the microorganism, which can be used to optimize the yield of a product. In this study, Central Composite Design (CCD) of Response Surface Methodology (RSM) was used to optimize the production of red pigment by Monascus purpureus FTC 5356 using the petioles of oil palm fronds (OPF) as a substrate in solid state fermentation (SSF). The data was analyzed using Design Expert Software. The optimum combination predicted via RSM was confirmed through experimental work. The interactions between three variables such as initial moisture content (%), initial pH value (pH), and peptone concentration (%) were studied and modelled. The statistical analysis of the results showed that the optimal conditions for red pigment production 47 AU/g with the biomass of 425.1 mg/g was at 55% initial moisture content, 3% of peptone, and at pH 3.Â The RSM results showed that the initial pH value had a significant effect on red pigment production (P-value <0.05). The validation of these results was also conducted by fermentation with predicted conditions and it was found that there was a discrepancy of 0.39% between the values of the experimental result and those of the predicted values. ABSTRAK: Keadaan persekitaran dan nutrien merupakan faktor-faktor penting dalam pertumbuhan mikroorganisma. Manipulasi faktor-faktor tersebut adalah kaedah terbaik bagi meningkatkan pertumbuhan mikroorganisma dan mengoptimumkan penghasilan produk. Kajian ini mengguna pakai Rekaan Gabungan Pusat (CCD) melalui Kaedah Tindak balas Permukaan (RSM) bagi penghasilan pigmen merah optimum oleh Monascus purpureus FTC 5356 menggunakan batang pelepah kelapa sawit (OPF) sebagai perumah dalam proses penapaian pepejal (SSF). Data telah dianalisis menggunakan perisian Design Expert. Gabungan parameter optimum seperti cadangan RSM telah disahkan secara eksperimen. Interaksi antara tiga pemboleh ubah seperti kandungan lembapan awal (%), nilai pH awal (pH), dan kepekatan pepton (%) telah dikaji dan dimodelkan. Analisis statistik menunjukkan penghasilan optimal pigmen merah adalah pada 47 AU/g dengan biomas sebanyak 425.1 mg/g, pada 55% lembapan awal, 3% pepton dan pada pH 3. Hasil keputusan RSM menunjukkan pH awal memberikan kesan signifikan kepada penghasilan pigmen merah (nilai P <0.05). Pengesahan analisis juga telah dijalankan melalui proses penapaian dan hasil ujikaji mendapati 0.39% lebih tinggi daripada nilai jangkaan.
DIFFERENTIAL APPROACH OF CONTINUOUS AND INTERMITTENT RENAL REPLACEMENT THERAPY APPLYING IN CARDIAC SURGERY ACUTE KIDNEY INJURY
Authors: Zakon K. , Romanova V. , Dudarenko V. , Arbuzova I. , Radchenko G. 
Published: June 2018

Background: In-hospital mortality of cardiac surgery patients with AKI is 3-7 times higher than those without AKI. This prospective observational study was dedicated to evaluate a differential approach ofapplying continuous and intermittent modalities of RRT in cardiac surgery patients.
Methods. One hundred and six adult cardiac surgery patients admitted hospital in 2008-2011 years, who had AKI and met inclusion criteria were allocated in CRRTor IRRTgroup.
Results. Observed in-hospital mortality of CRRT patients was significantly lower than predicted by APACHE II (р=0,01), in contrast with IRRT group. The in-hospital mortality of cardiac surgery patients with AKI and multiorgan dysfunction treated with CRRT was significantly lower than in patients treated with IRRT (32,5% vs 67,6%, р=0,012) and lower than predicted by APACHE II (32,5% vs 50%, р=0,025), while in IRRT group observed in-hospital mortality was significantly higher (67,6% vs 46%, р=0,012) than predicted.
Discussion. The complexity of the treatment of cardiac surgery patients with AKI and, especially, the subset with critical illness, could explain the lack of association between RRT modality and renal recovery and the in-hospital mortality of whole cohort, as well. This is a basis for differential and complementary applying ofdifferent RRT modalities according to the specific clinical situation.
Conclusions. Differential applying of continuous RRT modalities for the treatment of cardiac surgery patients with
AKI, as a component of MOF, and intermittent RRT for the patients, who have not multiorgan dysfunction, could contribute to reducing in-hospital mortality in this cohort.
NON-SPECIFIC PROTECTION FACTORS AND INFLAMMATORY MEDIATORS IN URINE OF CHILDREN WITH GLOMERULONEPHRITIS IN THE PRESENCE OF HERPESVIRAL INFECTION. NOTICE I. DETERMINATION OF SIGA AND MCP-1
Authors: Bagdasarova I. , Kruglikova I. , Kornilina E. , Kruglikov V. 
Published: June 2018

The aim of this work was the study of indicators characterizing the local immunity in patients with various forms of glomerulonephritis by presence of herpesviral infection.
Materials and methods. A quantitative estimation of the content of sIgA and MCP-1 in urine of 47patients with glomerulonephritis and 10 apparently healthy individuals was conducted.
Results. The decrease of sIgA level and reliable increase ofMCP-1 level were observed in urine of patients with glomerulonephritis. There was a significant increase in the level of these indices in hormone-resistant patients with nephrotic form of glomerulonephritis; in patients with severe course of the disease and also in the absence of remission. More such changes were found in children with active (reactivated) herpesviral infections.
Conclusion. There has been confirmed the importance of the study of immunologic parameters in the urine of patients with glomerulonephritis to characterize immunopathological process in the kidney and prognosis of the disease.
CORRECTION OF IRON DEFICIENCY IN CKD V HD PATIENTS WITH ANEMIA
Authors: Kolesnyk N. 
Published: June 2018

Hemodialysys results in loss of15-20 ml of blood or 5-7 mg of iron during each session or at least 1 g/ year. Additional iron may be lost through the gastrointestinal tract and during the menstruation in women. Excluding others possible reasons (genitourinary issues, laboratory investigations iron losses can amount minimum 1.7g per year). Dietary iron intake (0.5 – 1.0 g/year) only partially offsets iron deficit.
According to KDOQI Anemia Guidelines (2006), “the average iv iron needed to maintain a stable serum ferritin (SF) level and in that way neutral iron balance, appears to be range 22-65 mg/week; this amount corresponds with SFl > 200 mg/ at but the same time there is no sufficient evidence to recommend routine administration of iron if SF > 500 mg/l.
KDIGO Guidelines (2012) do not recommend routine use of iron supplementation in patients with transferrin saturation (TSAT) > 30% and SF > 500 mg/1.
Thereby the target SF and TSAT levels in CKD VD patients remain controversial and in practice are determined by specific clinical tasks requiring resolution: to increase hemoglobin level without erythropoiesis stimulating agents (ESA) administration, or to decrease ESA dose in its turn depends on specific patient’s iron status (absolute or functional iron deficit) and treatment period, – predialysis or dialysis.
CARRIAGE OF OPPORTUNISTIC MICROORGANISMS AND SURVIVAL OF PATIENTS WITH CHRONIC KIDNEY DISEASE STAGE 5 D
Authors: Shifris I. M. 
Published: June 2018

Despite of significant development of dialysis technology, mortality rates of CKD V D stage patients remain unsatisfactorily high. Next to cardiovascular diseases, infections are seems to be as a major causes of morbidity, hospitalization and mortality in this population. Staphylococcus aureus, especially MRSA, infections are a major cause of morbidity and hospitalization in CKD V D stage patients. Preceding MRSA colonization views as a risk factor for subsequent MRSA infections in future.
Aim. The aim of this study was to explore the effects of opportunistic pathogens colonization on survival in patients with CKD VD stage.
Materials and methods. This prospective cohort, open-label study included 255patients with CKD V D st. (198 HD and 57 PD patients). Patients were randomized into two groups, depending on the MRSA colonization history: first group (n=66) included patients with identified MRSA colonization and the second group (n=189) included patients with colonization of other opportunistic pathogens. The groups were representative according to gender, age, type of kidney affections and renal replacement therapy (RRT) modality. The endpoint was the total number of deaths. All cases, which took place from 01.08.2011 to 01.08.2016 year, were analyzed. The Kaplan-Meier method was perfomed for evaluation of survival.

Results. The analysis allowed to state that during analyzed period in total were 75 deaths: in the first group ofpatients - 32 (48.5%), and in the second - 43 (22.8%) cases; %2= 14,38, p = 0,000078; RR – 2,131, 95% ffl: 1,484-3,060.
The survival rate of patients was significantly lower in the first group, irrespective of RRT modality. The 3-years cumulative proportion surviving was 53% and 79% in the first (MRSApositive) and second groups, respectively; p< 0,001.
Conclusion. This study demonstrated that MRSA asymptomatic colonization has a significant negative effect on survival in the patient population with CKD VD stage
PHARMACO-ECONOMICBENEFITS OF CORRECTION OF ANEMIA WITH CONTINUOUS ERYTHROPOIETIN RECEPTOR ACTIVATOR:HEMODIALYSIS VSHEMODIAFILTRATION
Authors: Novakivskyy V. 
Published: June 2018

Anemia is an integral component of CKD. The prescribing of erythropoiesis stimulating agents (ESA) for the treatment of anemia constitutes a significant burden on health budgets. Using of continuous erythropoietin receptor activator (CERA) and convective techniques can improve health indicators and economic results.
The aim of the study was to evaluate the effects of hemodiafiltration treatment (HDF) on the pharmaco-economic efficiency of anemia treatment in comparison with haemodialysis (HD).
Methods. A prospective cross-sectional study involving 40 patients with CKD stage V who receiving dialysis treatment at the LLC “Fresenius Medical Care Ukraine ” Medical center in Cherkasy city. The follow-up period was 2 years. First, data was analyzed while patients received HD over a period of twelve months. Then, the same patients were evaluated during treatment with HDF for at least another twelve months.

Result. The average dose of CERA reduced from 60 mg to 44 mg per month (p = 0.002) after the transferred ofpatients to HDF treatment. There were a significantly increased the levels of Kt / V (p = 0.04) and serum albumin (p = 0.04). A higher number of patients achieved the target hemoglobin results.
Conclusions. The comparison of pharmaco-economic component of anemia correction with CERA cross-sectional study demonstrates significant advantages of HDF treatment compared to HD.
THE ROLE OF GUT INDIGENOUS MICROBIOTA IN THE DEVELOPMENT OF OXIDATIVE STRESS IN WOMEN WITH RECURRENT PYELONEPHRITIS
Authors: Stashevskyi N. V. , King L. V. , Myhal L. , Stepanova N. M. 
Published: June 2018

The aim of our study was to investigate the oxidative stress intensity depending on the content of Lactobacillus spp. in the colon of patients with recurrent pyelonephritis.
Material and methods. The observational study involved 60 women with chronic kidney disease (CKD) stage I-II: uncomplicated pyelonephritis, aged 39.5 ± 3.2 years. According to the quantitative content ofLactobacillus spp. in the patients’ intestine, the women were allocated into two groups: the first group of the patients (n = 34) had a deficit ofLactobacillus spp. in the intestine, and the second one (n = 26) had a normal content of Lactobacillus spp. Along with the standard diagnostic methods, we defined the content of malondialdehyde levels in the serum (MDAs) and erythrocytes (MDAe) spectrophotometrically as an indicator of lipid peroxidation. Such parameters as the concentration of ceruloplasmin (CP), transferrin (TR) and sulfhydryl groups (SH-groups) in the blood and total peroxidase activity (TPA) in erythrocyte were studied as the indicators of antioxidant system. In addition, we calculated of oxidative stress index (OSI).
Results. The analysis of lipid peroxidation defined a significant increase of MDA level in the serum (p = 0.008) and urine (p = 0.003) ofpatients with deficit of intestine lactobacillus spp. Moreover, in the patients ofGroup II we observed the high level of serum OSI (p = 0.03). By contrast, the TPA level was significantly reduced (p = 0.02).
Conclusions. The results of our work confirm the experimental studies data, which demonstrate the leading role of gut indigenous microbiota in the development of oxidative stress.
MORPHOFUNCTIONAL STATEOFKIDNEYS OF RATSAFTER INJECTIONOFPLACENTAL CRYOEXTRACT IN HEYMANN NEPHRITIS
Authors: Kondakov I.I. , Repin N.V. , Topchy I.I. , Marchenko L.N. , Vaskovich A.M. , Kirienko A.N. , Govorukha T.P. 
Published: June 2018

Изучение иммунного механизма поражения почек на модели нефрита Хеймана (НХ), вызывающего изменения сосудов почечных клубочков, их морфологических и морфометрических показателей, представляют значительный интерес
Цель работы: изучить влияние введения криоэкстракта аллогенной плаценты (КЭП) на функциональные и морфологические показатели почек при экспериментальном НХ.
Материал и методы. Крысы-самцы 4-х месячного возраста были разделены на 3 группы: I – интактные; 2 – животные с моделью НХ; 3 – животные с моделью НХ, которым на 28 день после иммунизации 3 раза за неделю внутримышечно вводили КЭП. Животных 2-й и 3-й групп выводили из эксперимента на 45 и 60 сутки. Исследованы биохимические, функциональные и морфологические показатели почек на всех стадиях развития НХ и после введения КЭП.
Результаты и их обсуждение. У животных 2 и 3 группы на 28-е сутки возрастало количество циркулирующих иммунных комплексов (ЦИК) в крови и иммунных депозитов на базальных мембранах клубочков, что сопровождалось нарушением их структуры и выделительной функции почек. Выявлены морфометрические различия в размерах клубочков в динамике НХ. Через 60 суток в ткани почек экспериментальных животных наблюдались признаки фокального мезангиального пролиферативного гломерулонефрита как при введении КЭП, так и без него. При введении КЭП отложения депозитов иммунных комплексов не обнаруживалось.
Выводы: При НХ на 28 сутки отмечено возрастание ЦИК в крови, отложение депозитов иммунных комплексов на базальных мембранах капилляров клубочков, сужение их просвета и пролиферация мезангиоцитов, что сопровождалось нарушением выделительной функции почек. Введение КЭП нормализовывало функцію почек, снижало к 45 суткам уровни комплемента ЦИК до показателей нормы.
AGE AND GENDER-SPECIFICS OF CARDIOVASCULAR MORTALITY IN HEMODIALYSIS PATIENTS
Authors: Shifris I. 
Published: June 2018

Cardiovascular diseases are the leading causes of death in hemodialysis (HD) patients. An analysis of the epidemiological evidence is the basis for the development of the measures and recommendations directed on reducing prevalence of fatal events, including cardiovascular.
Aim. The study of cardiovascular mortality in patients who received treatment for renal replacement therapy, based on the demographic and gender characteristics, type of kidney damage and duration of HD treatment.
Methods. Cardiovascular mortality causes in 89 patients who received programmed hemodialysis from January 1, 2007 to December 31, 2011 in Kyiv City Scientific and Practical Center of Nephrology and Dialysis were analyzed.
Results. Cardiovascular disease was the primary cause of death in the study population (64, 96 %). The annual cardiovascular mortality rate has a significant positive trend in the HD patients. It was ascertained its declining from 10,26% in 2007 to 6,69% in 2011.
About 40% of cardiovascular causes of death occurred during the first 90 days of HD treatment, 73,53% of them were among males.
The number of fatality authentically is higher for younger women with non- diabetic renal glomerular lesions and middle-aged patients with diabetes mellitus. CVD, as the cause of death, was significantly more often for males of middle age with the glomerular (diabetic and non-diabetic) diseases and of the elderly - with nonglomerular and non-diabetic glomerular origin.
Conclusion. According to the study the series of the regularities of the structure of cardiovascular mortality has been established. The significant association between the sex of the patient and the type of renal disease was identified in each age group.
STUDY OF INFLUENCE ZINC LEVEL IN BLOOD IN PATIENTS WITH CHRONIC PYELONEPHRITIS ON THE ACTIVITY OF INFLAMMATORY PROCESSES IN KIDNEYS
Authors: Lisnyanska I. S. 
Published: June 2018

Materials and methods: examined 67 patients with chronic pyelonephritis. All patients were determined level of zinc in the blood and levels of inflammatory cytokines, C-reactive protein in the urine.
Results. We found a significant decrease in the level of zinc in the blood of patients with chronic pyelonephritis (p<0.05). As for the performance of proinflammatory cytokines and CRP, we found a significant increase in IL-6 (p<0.001), TNF-α (p<0.001) and CRP (p<0.05) in the urine, indicating that the activity of the inflammatory response in situ. In applying the basic therapy levels of zinc in the blood were not significantly changed (p>0.05), and the application of basic therapy in combination with zinc sulfate significantly increased (p<0.05) and almost no difference in the values of group norms. Analyzing changes in cytokines and CRP in urine during treatment, we found a significant decrease in these indicators as the application of basic care and the application tested therapy, but the application of the second we found a greater decrease in these indicators, in some cases reaching values of group norms.
Conclusions. The preparation of zinc may be a promising direction of the treatment of chronic pyelonephritis, since increases this microelement in the blood and reduces the activity of inflammatory response in the kidney.
CHANGES OF NONESSENTIAL TRACE ELEMENTS LEVELS IN WHOLE BLOOD OF END STAGE RENAL DISEASE ADULT PATIENTS
Authors: Makarov O. O. , Pisarev E. O. , Sheiman B. S. , Kulizkiy M. V. , Peretyatko D. V. , Vihor V. O. , Prodanchuk M. G. 
Published: June 2018

Aims: Accumulation of trace elements occurs in conditions of decreased or termination of kidneys functions. In some conditions increased trace elements can obtain toxic features. On other hand, researches are showing that concentration of some trace elements could be decreased in ERSD patients, too. The most important factor affecting trace element concentration in ERSD patient is the degree of renal failure and using of replacement therapy.
Materials and methods: We determined the concentration of microelements (beryllium, boron, aluminum, vanadium, chromium, nickel, arsenic, rubidium, strontium, cadmium, cesium, barium, thallium and lead) in whole blood of 41 ESRD patients with chronic kidney disease stage VD, who were treated with hemodialysis / hemodiafiltration and in 61 conditionally healthy donors. Determination of whole bloods trace elements content was conducted using inductive coupled plasma mass- spectrometry (ICPMS).
Results: It is determinate that levels of beryllium, boron, aluminum, vanadium, chromium, strontium, cadmium, barium, thallium and lead is reliable increased in ESRD patients. Decreased levels are observed for nickel, arsenic, cesium and rubidium.
Conclusions: ESRD is accompanied with substantial and multidirectional changes of trace elements blood levels. During researches has shown that in ESRD patients processes of trace elements accumulation are prevailing over elimination ones.
THE QUALITY OF SLEEP IN PATIENTS WITH CKD UNDERGOING HEMODIALYSIS
Authors: Gonchar Y. 
Published: June 2018

The aim of study was to determine the quality of sleep and the quality of life in patients with CKD undergoing hemodialysis.

Український журнал нефрології та діалізу Оригінальні наукові роботи
Methods. This study included 70 patients undergoing hemodialysis (mean age 48,74+1,51 years, mean duration of dialysis 41,55+4,51 months). Sleep quality was measured using the Pittsburgh Sleep Quality Index (PSQI). Quality of life was measured using questionnaire SF–36.
Results. 41 (58,57%) patients with a global PSQI score >5 were considered as a “bad sleeper”. Poor sleep was associated with age (r 0,24, p <0,05), high BMI(r 0,27, p <0,05), low diuresis (r 0,25, p <0,05), increase interdialytic weight gain (r 0,26, p <0,05). There was relationship between the poor sleep and low scores of all scales SF–36.
Conclusions. Sleep disorders are common among patients with CKD undergoing hemodialysis. Poor sleep has a negative impact on quality of life.
INFLUENCE CHRONICKIDNEYDISEASEPROGRESSES TO QUALITYOFLIFE
Authors: Krasiuk I. V. 
Published: June 2018

Aim. To determine the characteristics of quality of life in patients with chronic kidney disease (CKD ) II–Vst.
Methods. 171 patients with CKD II–IVst, except for routine clinical and laboratory examinations, have been studied the quality of life (QOL) using a questionnaire assessing quality of life SF– 36.
Results. It was found most of the QOL was significantly worse with the progression of CKD. Total QOL indicators and indicator “effect of kidney disease”. most significantly reduced. Correlation most QOL with age, hemoglobin, blood pressure levels was revealed.
Conclusions. QOL decrease with the progression of CKD. Age, hemoglobin level, blood pressure affect QOL.
NON-SPECIFIC PROTECTION FACTORS AND INFLAMMATORY MEDIATORS IN URINE OF CHILDREN WITH GLOMERULONEPHRITIS IN THE PRESENCE OF HERPESVIRAL INFECTION NOTICE 2. DETERMINATION OF THE LEVEL OF IL-8 AND SLPI
Authors: Bagdasarova I. , Kruglikova I. , Kornilina E. , Kruglikov V. 
Published: June 2018

The aim of this work was the study of indicators characterizing the local immunity in patients with various forms of glomerulonephritis by presence of herpesviral infection.
Materials and methods. A quantitative estimation of the content of IL-8 and SLPI in urine of 47 patients with glomerulonephritis and 10 apparently healthy individuals was conducted.
Results. A insignificant increase of IL-8 level in urine was showed in patients with glomerulonephritis. The level of urinary SLPI was reliably higher than the control value, at the same time the majority ofpatients (74,0 %) did not exceed the upper limit of the physiological norm. The essential increase ofIL-8 level in urine was observed in 13 sick children, and of SLPI – in 12 patients. The increase of IL-8 and SLPI levels was observed more frequently in patients with hematuric form of glomerulonephritis, with nephrotic steroidoresistant variant of nephrotic form, in the absence of remission, and in the case of IL-8 - with severe course of the disease. More such changes were found in children with active (reactivated) herpesviral infections.
Conclusion. There has been confirmed the importance of the study of immunologic parameters in the urine of patients with glomerulonephritis to characterize immunopathological process in the kidney and prognosis of the disease.