bluuebunny/crossref_metadata_2025_split huggingface dataset:
Altering microtubule stability affects microtubule clearance and nuclear extrusion during erythropoiesis
Authors: Xie Songbo , Yan Bing , Feng Jie , Wu Yuhan , He Na , Sun Lei , Zhou Jun , Li Dengwen , Liu Min 
Published: April 2019

AbstractMammalian erythrocytes are highly specialized cells that have adapted to lose their nuclei and cellular components during maturation to ensure oxygen delivery. Nuclear extrusion, the most critical event during erythropoiesis, represents an extreme case of asymmetric partitioning that requires a dramatic reorganization of the cytoskeleton. However, the precise role of the microtubule cytoskeleton in the enucleation process remains controversial. In this study, we show that microtubule reorganization is critical for microtubule clearance and nuclear extrusion during erythropoiesis. Using a rodent anemia model, we found that microtubules were present in erythroblasts and reticulocytes but were undetectable in erythrocytes. Further analysis demonstrated that microtubules became disordered in reticulocytes and revealed that microtubule stabilization was critical for tubulin degradation. Disruption of microtubule dynamics using the microtubule‐stabilizing agent paclitaxel or the microtubule‐destabilizing agent nocodazole did not affect the efficiency of erythroblast enucleation. However, paclitaxel treatment resulted in the retention of tubulin in mature erythrocytes, and nocodazole treatment led to a defect in pyrenocyte morphology. Taken together, our data reveals a critical role for microtubules in erythrocyte development. Our findings also implicate the disruption of microtubule dynamics in the pathogenesis of anemia‐associated diseases, providing new insight into the pathogenesis of the microtubule‐targeted agent‐associated anemia frequently observed during cancer chemotherapy.
A lymph node metastasis‐related protein‐coding genes combining with long noncoding RNA signature for breast cancer survival prediction
Authors: Sui Yujie , Ju Chunyan , Shao Bin 
Published: April 2019

AbstractIntegrating protein‐coding gene (PCG) with long noncoding RNA (lncRNA) expression profiles, our aim is to identify a multidimensional transcriptome model that can predict individual prognosis of patients with breast cancer (BRCA). After diverse bioinformatics and statistical analyses, we obtained gene expression profiles of 1,016 BRCA samples from Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) database, and constructed a prognostic signature, which is composed of four PCGs (TFCP2, LRRC75B, PROSER2, and STOML1) and one lncRNA (AL355592.1). In the training set, the multidimensional transcriptome signature could part patients with BRCA into two groups with different survival, defined as high‐ and low‐risk group by Kaplan–Meier (KM) analysis (p < 0.001). In the other five validation datasets, the PCG‐lncRNA signature showed a similar predictive performance in BRCA by KM (p < 0.05). The prognostic independence for the PCG‐lncRNA model was verified by the multivariable Cox regression analysis. Because Chi‐squared test showed the signature was associated with lymph node metastasis status, stratification analysis found that it could further subdivide lymph node metastasis status more precisely in BRCA. The function analysis suggested that the genes from the signature were associated with immunity‐related pathways. In summary, we constructed a PCG‐lncRNA signature, which could accurately predict survival in patients with BRCA.
Effect of strontium substituted ß‐TCP associated to mesenchymal stem cells from bone marrow and adipose tissue on spinal fusion in healthy and ovariectomized rat
Authors: Salamanna Francesca , Giavaresi Gianluca , Contartese Deyanira , Bigi Adriana , Boanini Elisa , Parrilli Annapaola , Lolli Roberta , Gasbarrini Alessandro , Barbanti Brodano Giovanni , Fini Milena 
Published: April 2019

AbstractDespite alternatives to autogenous bone graft for spinal fusion have been investigated, it has been shown that osteoconductive materials alone do not give a rate of fusion comparable with autogenous bone. This study analyzed a strontium substituted ß‐tricalcium phosphate (Sr‐ßTCP) associated with syngeneic, unexpanded, and undifferentiated mesenchymal stem cells from bone marrow (BMSC) or adipose tissue (ADSC) as a new tissue engineering approach for spinal fusion procedures. A posterolateral fusion was performed in 15 ovariectomized (OVX) and 15 sham‐operated (SHAM) Inbred rats. Both SHAM and OVX animals were divided into three groups: Sr‐ßTCP, Sr‐ßTCP + BMCSs, and Sr‐ßTCP + ADSCs. Animals were euthanized 8 weeks after surgery and the spines evaluated by manual palpation, micro‐CT, and histology. For both SHAM and OVX animals, the fusion tissue in the Sr‐ßTCP + BMSCs group was more solid. This effect was significantly higher in OVX animals by comparing the Sr‐ßTCP + BMCSs group with Sr‐ßTCP + ADSCs. Radiographical score, based on micro‐CT 2D image, highlighted that the Sr‐ßTCP + BMCSs group presented a similar fusion to Sr‐ßTCP and higher than Sr‐ßTCP + ADSCs in both SHAM and OVX animals. Micro‐CT 3D parameters did not show significant differences among groups. Histological score showed significantly higher fusion in Sr‐ßTCP + BMSCs group than Sr‐ßTCP and Sr‐ßTCP + ADSCs, for both SHAM and OVX animals. In conclusion, our results suggest that addition of BMSCs to a Sr‐ßTCP improve bone formation and fusion, both in osteoporotic and nonosteoporotic animal, whereas spinal fusion is not enhanced in rats treated with Sr‐ßTCP + ADSCs. Thus, for conducting cells therapy in spinal surgery BMSCs still seems to be a better choice compared with ADSCs.
Brefeldin A impairs porcine oocyte meiotic maturation via interruption of organelle dynamics
Authors: Cui Zhaokang , Yu Lingzhu , Shi Yang Xiayan , Zhang Yu , Shi Xiaoyan , Li Yu , Chen Qiuju , Xiong Bo 
Published: April 2019

AbstractBrefeldin A (BFA) is a lactone antibiotic synthesized from palmitic acid by several fungi that could block anterograde transport of proteins from endoplasmic reticulum to Golgi apparatus by reversible disruption of the Golgi complex. Previous investigations have shown that BFA induces the apoptosis of cancer cells in mitosis and impairs asymmetric spindle positioning in meiosis. Here, we document that exposure to BFA in porcine oocytes compromises the meiotic maturation via disrupting both nuclear and cytoplasmic maturation. We found that BFA exposure collapsed the cytoskeleton assembly by showing the aberrant spindle organization with misaligned chromosomes and defective actin dynamics. Furthermore, the distribution of both mitochondria and cortical granules (CGs), two important indexes of cytoplasmic maturation of oocytes, was disturbed following BFA exposure. We finally validated that the localization of ovastacin, a component of CGs that is essential for the postfertilization removal of sperm‐binding sites in the zona pellucida, was also perturbed in BFA‐exposed oocytes, which might weaken their fertilization capacity. Collectively, these findings indicate that Golgi‐mediated protein transport is indispensable for the porcine oocyte meiotic maturation.
Messenger RNA and protein expression of tumor necrosis factor α and its receptors in human follicular granulosa cells
Authors: Salmassi Ali , Fattahi Amir , Simon Nora , Latifi Zeinab , Ghasemnejad Tohid , Nouri Mohammad , Schmutzler Andreas G. 
Published: April 2019

AbstractTo evaluate the concentration of tumor necrosis factor α (TNF‐α) and its soluble receptors (sTNFR I and II) in serum and follicular fluid (FF) at the time of oocyte retrieval and to detect expression of TNF‐α and its receptors by luteinized granulosa cells (GCs). In a cross‐sectional study and through an in vitro fertilization–intracytoplasmic sperm injection (IVF–ICSI) program, 81 women undergoing oocyte retrieval were recruited. Serum and FF were obtained from 81 women. GCs were pooled from 20 patients (from six different days of oocyte retrievals, 5–16 follicles per patient). TNF‐α and its soluble receptors concentration were determined by enzyme‐linked immunosorbent assay and also their expression by immune cytochemistry and reverse‐transcription polymerase chain reaction analysis. The median TNF‐α concentration in serum was 4.06 pg/ml (interquartile range [IQR], 3.71–6.14) and significantly higher than that in FF with 3.50 pg/ml (IQR, 3.05–5.01), p < 0.001. The sTNFR I and II levels in serum were lower and higher than FF, respectively. The TNF‐α levels in serum and FF of good responders were higher than low responders (p = 0.017 and 0.021, respectively). TNF‐α cut‐off level for low responders versus good responders was 4.174 pg/ml in serum with a pregnancy rate of 25.8% and 40% for below and above of this level, respectively (p = 0.19). For FF, the cut‐off value was 3.89 pg/ml. TNF‐α and its receptors were expressed by GCs. The presence of TNF‐α and its soluble receptors in serum and FF and their expression by GCs suggest an important role for this cytokine in ovarian function.
Demethoxycurcumin: A naturally occurring curcumin analogue for treating non‐cancerous diseases
Authors: Hatamipour Mahdi , Ramezani Mahin , Tabassi Sayyed Abolghasem Sajadi , Johnston Thomas P. , Sahebkar Amirhossein 
Published: April 2019

AbstractTurmeric extracts contain three primary compounds, which are commonly referred to as curcuminoids. They are curcumin, demethoxycurcumin (DMC), and bisdemethoxycurcumin. While curcumin has been the most extensively studied of the curcuminoids, it suffers from low overall oral bioavailability due to extremely low absorption as a result of low water solubility and instability at acidic pH, as well as rapid metabolism and clearance from the body. However, DMC, which lacks the methoxy group on the benzene ring of the parent structure, has much greater chemical stability at physiological pH and has been recently reported to exhibit antitumor properties. However, the treatment of noncancerous diseases with DMC has not been comprehensively reviewed. Therefore, here we evaluate published scientific literature on the therapeutic properties of DMC. The beneficial pharmacological actions of DMC include anti‐inflammatory, neuroprotective, antihypertensive, antimalarial, antimicrobial, antifungal, and vasodilatory properties. In addition, DMC's ability to ameliorate the effects of free radicals and an environment characterized by oxidative stress caused by the accumulation of advanced glycation end‐products associated with diabetic nephropathy, as well as DMC's capacity to inhibit the migration and proliferation of vascular smooth muscle cells following balloon angioplasty are also addressed. This review collates the available literature regarding the therapeutic possibilities of DMC in noncancerous conditions.
High expression of SLC38A1 predicts poor prognosis in patients with de novo acute myeloid leukemia
Authors: Li Yan , Shao Haigang , Da Zhenzhen , Pan Jinlan , Fu Bin 
Published: April 2019

AbstractThe glutamine amino acid transporter solute carrier family 38 member 1 (SLC38A1) is associated with the occurrence and progression of solid tumors. However, it has not yet been assessed in patients with hematologic malignancy. Herein, we investigated SLC38A1 expression and explored its clinical implications in acute myeloid leukemia (AML). The results showed that patients with high SLC38A1 expression had a lower mutation rate of NPM1 gene and higher incidence of adverse‐risk karyotype (p = 0.0010 and 0.0051, respectively). Patients with a high level of SLC38A1 expression presented significantly shorter overall survival in whole‐cohort, chemotherapy‐only, and non‐inv(16) AML (p = 0.0049, 0.0247, and 0.0005 respectively). Moreover, both univariate and multivariate analyses showed that high SLC38A1 expression was an independent unfavorable prognostic biomarker for AML (p = 0.0057 and 0.0483, respectively). In summary, our study revealed SLC38A1 as a valuable prognostic and predictive marker for AML. Further, glutamine transporter SLC38A1 might serve as a potential target for the development of novel therapeutic drugs in the treatment of AML.
Effects of extrusion types, screw speed and addition of wheat gluten on physicochemical characteristics and cooking stability of meat analogues
Authors: Samard Sasimaporn , Gu Bon‐Yeob , Ryu Gi‐Hyung 
Published: April 2019

AbstractBACKGROUNDPlant protein‐based products such as meat analogues have been receiving attention over the years. However, comparisons of product properties and mechanisms applied in the production of low‐ and high‐moisture meat analogues have not been reported. In this study, the effects of extrusion types (low‐ and high‐moisture extrusion cooking), absence or presence of added wheat gluten, as well as screw speed (150 and 200 rpm) on the physicochemical properties of meat analogues were evaluated. The mechanism of protein texturization of low‐ and high‐moisture meat analogues was studied.RESULTSExtrusion types and addition of wheat gluten had a major influence on physicochemical characteristics which were critical in controlling the fibrous texture of the final product, while screw speed had a minor impact on springiness only (P < 0.001). All high‐moisture meat analogues (HMMAs) were associated with a higher integrity index and greater stability of springiness and cutting strength than low‐moisture meat analogues (LMMAs) using the same formula and screw speed, while the nitrogen solubility index of HMMAs was lower. Based on the physicochemical properties determined, the higher cross‐link formation in HMMAs is proposed to occur in the cooling die section.CONCLUSIONOur findings show that the utilization of high‐moisture extrusion cooking and the incorporation of wheat gluten into the formula at 400 g kg−1 could impart a fibrous and compact structure to extrudates similar to that of actual muscle meat, with a greater integrity index and texture stability. © 2019 Society of Chemical Industry
Outcomes analysis of Goldilocks mastectomy and breast reconstruction: A single institution experience of 96 cases
Authors: Chaudhry Arif , Oliver Jeremie D. , Vyas Krishna S. , Alsubaie Saad A. , Manrique Oscar J. , Martinez‐Jorge Jorys 
Published: April 2019

AbstractBackgroundThe Goldilocks mastectomy procedure involves local contouring of completely autologous breast tissue created by preserving and de‐epithelializing the residual mastectomy flaps. The purpose of this study was to provide outcomes data for 96‐Goldilocks mastectomy procedures analyzing indications, complications, relevant comorbidities, and adjuvant cancer treatment impacting reconstructive and aesthetic outcomes.MethodsComprehensive review of every patient who underwent Goldilocks mastectomy from 2012 to 2018 included relevant medical and surgical comorbidities, as well as complication profiles. Aesthetic outcomes were also assessed in those with postoperative imaging available.ResultsA total of 53 patients (96 breasts) were included in this study. Bilateral cases consisted of 81.1% of the total cohort (n = 86 cases), and 18.9% (n = 10 cases) were unilateral procedures. Mean age at the time of reconstruction was 55.8 (33‐77) years. Mean body mass index (BMI) at the time of reconstruction was 33.7 (19.2‐54.6). The overall complication rate was 9.38% (seroma = 2, hematoma = 1, cellulitis = 2, wound dehiscence = 3, and operating room take back = 1).ConclusionsGoldilocks breast reconstruction is a safe, effective option in patients with higher than average BMI or excess local breast tissue, or in patients meeting these criteria preferring a single‐stage reconstruction. This study qualifies its use in patients with higher than average risk factors for more extensive reconstructive modalities.
Preclinical imaging of epidermal growth factor receptor with ABY‐029 in soft‐tissue sarcoma for fluorescence‐guided surgery and tumor detection
Authors: Samkoe Kimberley S. , Sardar Hira S. , Bates Brent D. , Tselepidakis Niki N. , Gunn Jason R. , Hoffer‐Hawlik Kevin A. , Feldwisch Joachim , Pogue Brian W. , Paulsen Keith D. , Henderson Eric R. 
Published: April 2019

AbstractBackground and ObjectivesFluorescence‐guided surgery using epidermal growth factor receptor (EGFR) targeting has been performed successfully in clinical trials using a variety of fluorescent agents. We investigate ABY‐029 (anti‐EGFR Affibody® molecule labeled with IRDye 800CW) compared with a small‐molecule perfusion agent, IRDye 700DX carboxylate, in a panel of soft‐tissue sarcomas with varying levels of EGFR expression and vascularization.MethodsFive xenograft soft‐tissue sarcoma cell lines were implanted into immunosuppressed mice. ABY‐029 and IRDye 700DX were each administered at 4.98 μM. Fluorescence from in vivo and ex vivo (fresh and formalin‐fixed) fixed tissues were compared. The performance of three fluorescence imaging systems was assessed for ex vivo tissues.ResultsABY‐029 is retained longer within tumor tissue and achieves higher tumor‐to‐background ratios both in vivo and ex vivo than IRDye 700DX. ABY‐029 fluorescence is less susceptible to formalin fixation than IRDye 700DX, but both agents have disproportional signal loss in a variety of tissues. The Pearl Impulse provides the highest contrast‐to‐noise ratio, but all systems have individual advantages.ConclusionsABY‐029 demonstrates promise to assist in wide local excision of soft‐tissue sarcomas. Further clinical evaluation of in situ or freshly excised ex vivo tissues using fluorescence imaging systems is warranted.
What if something unexpected happens to my brand? Spillover effects from positive and negative events in a co‐branding partnership
Authors: Koschate‐Fischer Nicole , Hoyer Wayne D. , Wolframm Christiane 
Published: April 2019

AbstractBrands sometimes enter into co‐branding agreements and major events can occur to one of the brands which can have either negative or positive consequences for the brands involved in the partnership. The current study investigates as yet unanswered research questions regarding whether and how positive and negative events happening to one brand in a co‐branding partnership affect the brand equities of the other brands in the partnership. The authors provide and test a new integrated attribution‐diagnosticity framework to explain the process that underlies the magnitude of these spillover effects. Findings show that while positive and negative event‐related spillover effects occur between the co‐brand and both parent brands, they were surprisingly absent between the parent brands. Further, the (a)symmetry of parent brand equity before the event influences the magnitude of event‐related spillover the parent brands receive from the co‐brand. Theoretical and practical implications of these findings are discussed.
A size‐dependent Bernoulli‐Euler beam model based on strain gradient elasticity theory incorporating surface effects
Authors: Fu Guangyang , Zhou Shenjie , Qi Lu 
Published: April 2019

AbstractIn this paper, a size‐dependent model for Bernoulli‐Euler beam is developed by using a general strain gradient theory and a surface elasticity theory. The general strain gradient theory is used to capture the strain gradient effects while the surface elasticity theory is applied to explain the surface effects. As comparison, the approximate theories including the couple stress theory and the simplified strain gradient theory are also used to describe the strain gradient effects. The Hamilton's principle is used to derive the governing equations, boundary conditions and initial conditions. The static bending and free vibration behaviour of nanobeams with different boundary conditions are analysed. Compared with the results predicted by the couple stress theory with surface effects and the simplified strain gradient theory with surface effects, because the general strain gradient theory contains the influences of all strain gradients, the deflection predicted by the general strain gradient theory with surface effects is smallest while the natural frequency is highest. The general strain gradient theory reflects size effects more accurately.
Universal Graphs and Good for Games Automata: New Tools for Infinite Duration Games
Authors: Colcombet Thomas , Fijalkow Nathanaël 
Published: April 2019

AbstractIn this paper, we give a self contained presentation of a recent breakthrough in the theory of infinite duration games: the existence of a quasipolynomial time algorithm for solving parity games. We introduce for this purpose two new notions: good for small games automata and universal graphs.The first object, good for small games automata, induces a generic algorithm for solving games by reduction to safety games. We show that it is in a strong sense equivalent to the second object, universal graphs, which is a combinatorial notion easier to reason with. Our equivalence result is very generic in that it holds for all existential memoryless winning conditions, not only for parity conditions.
Two-Way Parikh Automata with a Visibly Pushdown Stack
Authors: Dartois Luc , Filiot Emmanuel , Talbot Jean-Marc 
Published: April 2019

AbstractIn this paper, we investigate the complexity of the emptiness problem for Parikh automata equipped with a pushdown stack. Pushdown Parikh automata extend pushdown automata with counters which can only be incremented and an acceptance condition given as a semi-linear set, which we represent as an existential Presburger formula over the final values of the counters. We show that the non-emptiness problem both in the deterministic and non-deterministic cases is . If the input head can move in a two-way fashion, emptiness gets undecidable, even if the pushdown stack is visibly and the automaton deterministic. We define a restriction, called the single-use restriction, to recover decidability in the presence of two-wayness, when the stack is visibly. This syntactic restriction enforces that any transition which increments at least one dimension is triggered only a bounded number of times per input position. Our main contribution is to show that non-emptiness of two-way visibly Parikh automata which are single-use is NExpTime-c. We finally give applications to decision problems for expressive transducer models from nested words to words, including the equivalence problem.
Trees in Partial Higher Dimensional Automata
Authors: Dubut Jérémy 
Published: April 2019

AbstractIn this paper, we give a new definition of partial Higher Dimension Automata using lax functors. This definition is simpler and more natural from a categorical point of view, but also matches more clearly the intuition that pHDA are Higher Dimensional Automata with some missing faces. We then focus on trees. Originally, for example in transition systems, trees are defined as those systems that have a unique path property. To understand what kind of unique property is needed in pHDA, we start by looking at trees as colimits of paths. This definition tells us that trees are exactly the pHDA with the unique path property modulo a notion of homotopy, and without any shortcuts. This property allows us to prove two interesting characterisations of trees: trees are exactly those pHDA that are an unfolding of another pHDA; and trees are exactly the cofibrant objects, much as in the language of Quillen’s model structure. In particular, this last characterisation gives the premisses of a new understanding of concurrency theory using homotopy theory.