bluuebunny/crossref_metadata_2025_split huggingface dataset:
Force-induced decline of FOXM1 in human periodontal ligament cells contributes to osteoclast differentiation
Authors: Li Qian , Zhang Jianyun , Liu Dawei , Liu Yunan , Zhou Yanheng 
Published: March 2019

ABSTRACT

Objectives:
To investigate whether Forkhead family transcription factors are responsive to mechanical force and the resulting influence on the osteoclast differentiation mediated by human periodontal ligament cells (PDLCs).

Materials and Methods:
A high-throughput RNA sequencing assay was performed in compressive force–stimulated and control human PDLCs. Alteration of FOXM1, a member of the Forkhead family transcription factors, was further confirmed by Western blotting and quantitative reverse-transcription polymerase chain reaction. Expression of FOXM1 was inhibited by either small interfering RNA (siRNA) transfection or addition of its specific inhibitor Siomycin A. Then, cells were exposed to compressive force and co-cultured with the murine macrophage cell line Raw264.7, followed by tartrate-resistant acid phosphatase staining assay. Expression changes of receptor activator of nuclear factor κB ligand (RANKL) and osteoprotegetin (OPG) caused by FOXM1 suppression were measured. Alkaline phosphatase (ALP) staining, ALP activity assay, and crystal violet staining assay were performed after FOXM1 inhibition.

Results:
FOXM1 transcription decreased after mechanical stimulation in PDLCs. Inhibition of FOXM1 promoted force-induced osteoclast differentiation of RAW264.7 and upregulated the RANKL/OPG ratio in PDLCs. Interference of FOXM1 led to promoted osteogenic differentiation but decreased proliferation of PDLCs.

Conclusions:
FOXM1 is a novel mechano-responsive gene in human PDLCs. Suppressing FOXM1 expression could promote osteoclast differentiation as well as RANKL/OPG in human PDLCs. FOXM1 also plays a role in controlling PDLC differentiation and proliferation capacity.
Asymmetric dental arch treatment with Forsus fatigue appliances: Long-term results
Authors: Akın Mehmet , Erdur Emire Aybuke , Öztürk Onur 
Published: March 2019

ABSTRACT

Objectives:
To investigate the treatment efficacy and follow-up stability of the asymmetric Forsus appliance by evaluating longitudinal changes in dental arch asymmetry on digital dental models from 21 patients.

Materials and Methods:
Maxillary and mandibular reference lines were used for measurements of intra-arch asymmetry at pretreatment (T1), posttreatment (T2), and 4.2 years after treatment (T3). Maxillary and mandibular measurements were performed relative to the dental midline and anterior reference line on digital dental models. To determine the amount of asymmetry between the Class I and Class II sides of a given arch, all maxillary and mandibular parameters were measured on each side of the model separately. Repeated-measures analysis of variance/paired sample t-tests were performed to evaluate dental arch asymmetries at the P &lt; .05 level.

Results:
The alveolar transverse dimensions of the posterior segment of both arches were increased during treatment (P &lt; .05) and remained stable during the retention period. Class II subdivision malocclusion was caused by distal positioning of the mandibular canine, premolars, and first molar on the Class II side (P &lt; .05). Asymmetry was resolved by treatment with asymmetric Forsus appliances. The resolved asymmetry remained stable over the long term. There were no significant differences between T2 and T3 (P &gt; .05).

Conclusions:
The asymmetric Forsus appliance can be used to treat dental arch asymmetry in patients with Class II subdivision malocclusions.
Phototherapy is unable to exert beneficial effects on orthodontic tooth movement in rat molars
Authors: Friedrichsdorf Simone P. , Zaniboni Emily , Simões Alyne , Arana-Chavez Victor E. , Dominguez Gladys C. 
Published: March 2019

ABSTRACT

Objectives:
To investigate the effects of irradiation lasers and light-emitting diode (LED) light on root resorption in rat molars during orthodontic tooth movement (OTM).

Materials and Methods:
Twenty-one 12-week-old Wistar male rats were divided into three groups: OTM only (control [CG]), OTM and LED irradiation (DG), and OTM and low laser irradiation therapy (LG). The distance between the first and second molars was used to evaluate the amount of tooth movement. The mesial surfaces of the distopalatal roots were analyzed by scanning electron microscopy, and the area of the resorption was calculated.

Results:
Statistical analysis showed a decreased amount of tooth movement in the exposed DG or LG compared with CG, which was statistically significant (P = .031 and P = .004, respectively). However, when the DG and LG groups were compared, no statistically significant differences were found (P = .504). The root resorption areas were similar between CG and DG. However, statistically significant differences were found between LG and CG (P = .014) and LG and DG (P = .038).

Conclusions:
Phototherapy did not enhance tooth movement, while infrared laser irradiation did increase root resorption.
Early Intervention for Diabetes in Medical and Surgical Inpatients Decreases Hyperglycemia and Hospital-Acquired Infections: A Cluster Randomized Trial
Authors: Kyi Mervyn , Colman Peter G. , Wraight Paul R. , Reid Jane , Gorelik Alexandra , Galligan Anna , Kumar Shanal , Rowan Lois M. , Marley Katie A. , Nankervis Alison J. , Russell David M. , Fourlanos Spiros 
Published: March 2019

OBJECTIVE
To investigate if early electronic identification and bedside management of inpatients with diabetes improves glycemic control in noncritical care.

RESEARCH DESIGN AND METHODS
We investigated a proactive or early intervention model of care (whereby an inpatient diabetes team electronically identified individuals with diabetes and aimed to provide bedside management within 24 h of admission) compared with usual care (a referral-based consultation service). We conducted a cluster randomized trial on eight wards, consisting of a 10-week baseline period (all clusters received usual care) followed by a 12-week active period (clusters randomized to early intervention or usual care). Outcomes were adverse glycemic days (AGDs) (patient-days with glucose &lt;4 or &gt;15 mmol/L [&lt;72 or &gt;270 mg/dL]) and adverse patient outcomes.

RESULTS
We included 1,002 consecutive adult inpatients with diabetes or new hyperglycemia. More patients received specialist diabetes management (92% vs. 15%, P &lt; 0.001) and new insulin treatment (57% vs. 34%, P = 0.001) with early intervention. At the cluster level, incidence of AGDs decreased by 24% from 243 to 186 per 1,000 patient-days in the intervention arm (P &lt; 0.001), with no change in the control arm. At the individual level, adjusted number of AGDs per person decreased from a mean 1.4 (SD 1.6) to 1.0 (0.9) days (−28% change [95% CI −45 to −11], P = 0.001) in the intervention arm but did not change in the control arm (1.8 [2.0] to 1.5 [1.8], −9% change [−25 to 6], P = 0.23). Early intervention reduced overt hyperglycemia (55% decrease in patient-days with mean glucose &gt;15 mmol/L, P &lt; 0.001) and hospital-acquired infections (odds ratio 0.20 [95% CI 0.07–0.58], P = 0.003).

CONCLUSIONS
Early identification and management of inpatients with diabetes decreased hyperglycemia and hospital-acquired infections.
ANALISIS TINGKAT KEPUASAN MAHASISWA TERHADAP METODE DAN MEDIA PEMBELAJARAN PADA MATA KULIAH MANAJEMEN SUMBERDAYA MANUSIA SEMESTER 2 KELAS A DI JURUSAN MANAJEMEN
Authors: Harmen Hilma , Agustini Fauzia , Aprinawati Aprinawati 
Published: March 2019

Penelitian ini bertujuan untuk menganalisis Tingkat Perbandingan Kepuasan Mahasiswa Terhadap Media dan Metode Pembelajaran Pada Mata Kuliah Pembelajaran Pada Mata Kuliah Manajemen SDM kelas 2 A Tahun Ajaran 2016 -2017 di jurusan Manajemen Fakultas Ekonomi Unimed. Yang menjadi variable bebas dalam penelitian ini adalah Media Pembelajaran dan Metode Pembelajaran. Sedangkan variable terikatnya adalah Kepuasan Mahasiswa. Populasi Penelitian adalah mahasiswa di Jurusan Manajemen semester 2 (dua) kelas A tahun ajaran 2016-2017 sebanyak kurang lebih 40 orang. Sampel yang digunakan adalah total samping. Pengumpulan data menggunakan angket atau kuesioner. Teknik analisis data dalam penelitian ini menggunakan Analisis deskriptif. Mahasiswa merasa puas terhadap media yang digunakan di dalam proses pembelajaran matakuliah MSDM. Dengan tingkat persentasi jawaban setuju pada tiap-tiap indikator antara 78% - 95%. Mahasiswa merasa puas terhadap metode yang di gunakan Dosen dalam pembelajaran mata kuliah Manajemen SDM, Dengan tingkat persentasi jawaban setuju pada tiap-tiap indikator antara 67% - 95%. Kata Kunci : Kepuasan, Metode pembelajaran, Media pembelajaran
Current Needs and Difficulties in English Language Skill Faced by The Tourism Students
Authors: . Ratnah 
Published: March 2019

Recently, the development of tourism is increasing sharply and hotel industry has important role to support the tourism development in terms of providing the comfortable accommodation and excellent services and English language competencies should be possessed by the hotel employees in order to provide excellent services. However, some hotel employees still have low competence in English language skill. The purpose of this study is to find out the language skills that are mostly needed and categorized as the problems by the hotel employees in their work. This study employed quantitative study by using questionnaire and interview as the method of data collection. The sample of this study was taken by using purposive sampling by taking 30 tourism students who have just completed their six-month internship program in the industry. Based on the results of data analysis, it has been found that most language skills (listening, speaking, and writing) are categorized “High” used by the hotel employees, as their score between 3.41-4.20. While reading skills are categorized “Moderate” as its mean score between 2.61-3.40. And also All the language skills (listening, speaking, reading, and writing) are categorized “High” as problems faced by the hotel employees, as their score between 3.41-4.20.
Hepatic computed tomography and cholangiography by use of gadoxetic acid in healthy cats
Authors: Pilton Joanna L. , Chau Jennifer , Foo Timothy S. , Hall Evelyn J. , Martinez-Taboada Fernando , Podadera Juan M. , Makara Mariano A. 
Published: March 2019

Abstract

OBJECTIVE
To evaluate 3 doses of gadoxetic acid (Gd-EOB-DPTA) for hepatic CT and cholangiography in cats and to determine optimal timing for hepatobiliary image acquisition and evaluation of the contrast-enhanced hepatobiliary anatomy.

ANIMALS
6 healthy cats.

PROCEDURES
Cats were anesthetized; sequential CT scans were performed 0, 5, 25, 45, 65, and 85 minutes after IV administration of Gd-EOB-DTPA at low (0.0125 mmol/kg), medium (0.1 mmol/kg), and high (0.3 mmol/kg) doses. Hepatobiliary enhancement for each dose was objectively assessed over time and by use of a subjective semiquantitative visual assessment score.

RESULTS
No contrast-related adverse effects were detected. Each increase in dose of contrast medium resulted in a significant increase in HU across the hepatobiliary system. The liver had a significantly higher number of HU at 45 minutes, with homogenous enhancement at all doses of contrast medium. Contrast-enhanced cystic and bile duct HU were significantly higher and maximal at 65 minutes. Contrast-enhanced gallbladder HU did not plateau by 85 minutes. At a high dose of contrast medium, 12 of 60 (20%) biliary tract scores indicated no enhancement, 34 (57%) indicated poor enhancement, and 14 (23%) indicated moderate enhancement. No cat had excellent enhancement of the biliary tract at any dose.

CONCLUSIONS AND CLINICAL RELEVANCE
Gd-EOB-DTPA–enhanced hepatic CT and cholangiography in cats were safely performed and provided good hepatic enhancement but poor to moderate enhancement of the biliary tract. This technique may be useful for assessing the liver parenchyma in cats, but its value for assessing the biliary tract is questionable.
Predictive values of serum uric acid and Alanine-aminotransferase for fatty liver index in Montenegrin population
Authors: Klisic Aleksandra , Kavaric Nebojsa , Ninic Ana 
Published: March 2019

Summary

Background
Alanine-aminotransferase (ALT) and uric acid cut-off levels used in non-alcoholic fatty liver disease (NAFLD) diagnosis are advised to be lowered. Due to contradictory results on the utility of both these biomarkers for NAFLD screening, we aimed to determine their cut-off levels that can be applied to Montenegrin population with the fatty liver disease.

Methods
A total of 771 volunteers were enrolled. A fatty liver index (FLI) score ≥60 was used as proxy of NAFLD. The receiver operating characteristic curve analysis with the area under the curve (AUC) was used to determine the cut-off values of ALT and uric acid associated with FLI ≥60.

Results
ALT was independent predictor of FLI in both men and women, whereas serum uric acid was its independent predictor only in women. Lower cut-off levels of ALT are associated with the increased prevalence of NAFLD [i.e., ALT was 19 IU/L (AUC=0.746, sensitivity 63%, specificity 72%, P<0.001) in women and 22 IU/L (AUC=0.804, sensitivity 61%, specificity 95%, P<0.001) in men]. The cut-off value for uric acid was 274 μmol/L (AUC=0.821, sensitivity 68%, specificity 82%, P<0.001) in women.

Conclusions
Lower cut-off levels of ALT in both genders, and serum uric acid in females, can be reliable predictors of the FLI.
Effects of an eight-week exercise program on parameters of the lipid profile of female students
Authors: Juhas Irina , Skof Branko , Popović Dejana , Matić Milan , Janković Nenad 
Published: March 2019

Summary

Background
Increased lipid levels are one of the major risk factors for the development of cardiovascular diseases. The aim of the current study was to evaluate the effect of short-term (8 weeks) aerobic exercise of moderate to vigorous intensity on lipid profile in young healthy females.

Methods
27 female students (mean age 20.5 ± 1 year) completed 8 weeks of aerobic training that included two exercise sessions of continuous aerobic activity of moderate intensity (running 35–60 min) and one exercise session with interval training of vigorous intensity. Intervention aerobic capacity and lipid profile were examined before and after the exercise.

Results
Exercise intervention has caused a decrease of low density lipoprotein levels per 9.8% (from 2.52 ± 0.47 to 2.27 ± 0.53 mmol/L; p < 0.001) and significant improvement of high density lipoprotein (HDL) levels per 22.7% (from 1.29 ± 0.24 to 1.59 ± 0.24 mmol/L; p < 0.001), total cholesterol/HDL ratio per 17.2 % and aerobic capacity (VO2 max) per 3.8 % (43.9 ± 3.7 to 45.56 ± 3.63 mLO2/kg/min).

Conclusions
The results support the hypothesis that a short-term aerobic exercise intervention of moderate to vigorous intensity may have significant effects on blood lipid profile in young healthy females.
Diagnostic benefits of mindin as a prostate cancer biomarker
Authors: Hanousková Lenka , Řezáč Jakub , Veselý Štĕpán , Průša Richard , Kotaška Karel 
Published: March 2019

Summary

Background
It has been shown that decreased expression and activity of extracellular matrix protein mindin correlate with various types of cancers including breast, colon and lung cancers. The aim of the presented study was to investigate the serum mindin levels in prostate cancer.

Methods
Mindin concentrations in serum were measured in 56 patients with prostate cancer (mean age 68 years) and in control group of 29 healthy men (mean age 64 years) using commercially available enzymatic immunoassay (Cusabio, WuHan, China). The patients were divided with respect to the severity of the disease into two groups according to the EAU guidelines (stage 1, 2 – less severe tumours, stage 3, 4 – severe tumours).

Results
Serum mindin concentrations were significantly elevated in the group of healthy individuals unlike in the patients with prostate cancer (2.12 ng/mL vs 0.78 ng/mL, with P=0.0007, AUC=0.705). Patients with less severe tumours (stage 1, 2) and severe tumours (stage 3, 4) had significantly decreased levels of S-mindin as well (P=0.0037), although the difference in serum mindin concentrations between the patients with less severe and severe tumours was not significant.

Conclusions
Concentrations of mindin were decreased in patients with prostate cancer and reduced in patients with less severe prostate cancer as well. Mindin appears to be a promising diagnostic marker useful in the diagnosis of prostate cancer.
Changes in the blood fatty-acid profile associated with oxidative-antioxidant disturbances in coronary atherosclerosis
Authors: Ragino Yuliya I. , Shramko Viktoriya S. , Stakhneva Ekaterina M. , Chernyak Elena I. , Morozov Sergey V. , Shakhtshneider Elena V. , Polonskaya Yana V. , Shcherbakova Liliia V. , Chernyavskiy Alexander M. 
Published: March 2019

SummaryBackgroundThe objective of this work was to study the profile of fatty acids and to search for associations of fatty acids with oxidative-antioxidant parameters and an oxidative-inflammatory biomarker (lipoprotein-associated phospholipase A2) in men with coronary atherosclerosis and coronary heart disease.MethodsAnalysis of 20 fatty acids was performed in 60 men with angiographically confirmed coronary atherosclerosis and coronary heart disease and in a control group of men without coronary heart disease. Serum fatty-acid content was evaluated by high-performance gas-liquid chromatography. The blood levels of oxidative stress, total antioxidative defence, and lipoprotein-associated phospholipase 2 were analyzed.ResultsIn the group of men with coronary atherosclerosis the levels of myristic and palmitic fatty acids were higher by 59% and 22%, respectively. An increase in the weight percentage of monounsaturated fatty acids was noted, such as palmitoleic, oleic, and octadecenic. Significantly lower levels of polyunsaturated fatty acids, such as linolic, eicosadienoic, eicosatrienoic, arachidonic, eicosapentaenoic, γ-linolenic, docosapentaenoic, and docosahexaenoic were detected in the group with coronary atherosclerosis. The lipoprotein-associated phospholipase A2 level was higher by 48%. Oxidative stress was higher by 17%, and the total antioxidant defence in serum was lower by 45%. We found correlations between fatty acids and oxidative-antioxidative alterations. The relative risk of vulnerable atherosclerotic plaques correlated with increased levels of palmitic, stearic, oleic, and linolic fatty acids.ConclusionsSignificant alterations in the profile of fatty acids are associated with oxidative-antioxidative alterations and are accompanied by an increase in free-radical formation, which can probably serve as a risk factor of atherosclerosis.
Characteristics of selected active substances used in disinfectants and their virucidal activity against ASFV
Authors: Juszkiewicz Małgorzata , Walczak Marek , Woźniakowski Grzegorz 
Published: March 2019

Abstract
African swine fever (ASF), caused by African swine fever virus (ASFV), is currently one of the most important and serious diseases of pigs, mainly due to the enormous sanitary and socio-economic consequences. It leads to serious economic losses, not only because of the near 100% mortality rate, but also through the prohibitions of pork exports it triggers. Currently neither vaccines nor safe and effective chemotherapeutic agents are available against ASFV. The disease is controlled by culling infected pigs and maintaining high biosecurity standards, which principally relies on disinfection. Some countries have approved and/or authorised a list of biocides effective against this virus. This article is focused on the characteristics of chemical substances present in the most popular disinfectants of potential use against ASFV. Despite some of them being approved and tested, it seems necessary to perform tests directly on ASFV to ensure maximum effectiveness of the disinfectants in preventing the spread of ASF in the future.
Genetic diversity of Campylobacter jejuni isolated from the poultry food chain
Authors: Wieczorek Kinga , Osek Jacek 
Published: March 2019

Abstract

Introduction:
Campylobacter jejuni is one of the most frequently reported causes of foodborne bacterial enteric disease worldwide. The main source of these microorganisms is contaminated food, especially of poultry origin. There are several molecular methods for differentiation of Campylobacter isolates at the subgenus level, and one of these is porA-typing based on the sequencing of the major outer-membrane protein (MOMP) encoding gene. The aim of the study was to test the molecular relationship of C. jejuni strains isolated at different points along the poultry food chain and assess the population structure of the isolates.

Material and Methods: A total of 451 C. jejuni were used in the study, and a DNA fragment of 630 bp of the MOMP encoding gene was amplified and sequenced.

Results: One hundred and ten sequence types were identified, with 69 (62.7%) unique to the isolates' origin and 30 not present in the database. The most prevalent nucleotide variant 1 was detected in 37 (8.2%) strains. These isolates were identified in all poultry sources tested, especially in faeces (15 isolates) but also in poultry carcasses and meat (11 isolates in each).

Conclusion: The porA typing method was highly discriminative for C. jejuni of poultry origin since the Simpson's diversity index (D) achieved a value of 0.876, indicating considerable diversity in the bacterial population tested. The method may be further used for epidemiological investigation purposes.
Retrospective analysis of indications and complications related to implantation of permanent pacemaker: 25 years of experience in 31 dogs
Authors: Noszczyk-Nowak Agnieszka , Michałek Marcin , Kapturska Karolina , Cepiel Alicja , Janiszewski Adrian , Pasławski Robert , Skrzypczak Piotr , Pasławska Urszula 
Published: March 2019

Abstract

Introduction: Pacemaker implantation is the only effective symptomatic treatment for life-threatening bradyarrhythmias. Major complications observed after implantation of cardiac pacemakers include lead dislocation, loss of pulse generator function, and inadequate stimulation. The aim of this retrospective single-centre study was to analyse the indications for pacemaker implantation and the incidence and types of complications associated with this procedure in dogs treated for symptomatic bradyarrhythmia.

Material and Methods: The retrospective analysis included 31 dogs with symptomatic bradyarrhythmia, implanted with permanent cardiac pacemakers in 1992–2017. The list of analysed variables included patient age, breed, sex, indication for pacemaker implantation, comorbidities, and the incidence of procedure-related complications along with the type thereof.

Results: The most common indication for pacemaker implantation was 3rd degree AVB, followed by SSS, advanced 2nd degree AVB, and PAS. Pacemaker implantation was associated with a 35% overall complication rate and 6.45% periprocedural mortality. There were no significant differences in terms of procedure-related complications with regard to age, sex, breed, indications for pacemaker implantation, or comorbidities.

Conclusions: Cardiac pacing is the only effective treatment of symptomatic bradycardia, but as an invasive procedure, may pose a risk of various complications, including death.