bluuebunny/crossref_metadata_2025_split huggingface dataset:
Safety and efficacy of outpatient intravenous diuresis in decompensated heart failure: a systematic review
Authors: Kalkur Roshni S. , Hintz John P. , Pathangey Girish , Manning Katharine A. 
Published: November 2024

IntroductionHeart failure (HF) burdens the US healthcare system, with annual costs exceeding $30 billion. Outpatient intravenous (OP IV) diuresis in clinic or home settings may potentially improve outcomes and reduce costs, though limited data exists. This systematic review evaluates the safety, efficacy, and outcomes of OP IV diuresis in managing decompensated HF as a hospitalization alternative.MethodsFollowing PRISMA 2020 guidelines, this systematic review used MeSH terms in MEDLINE, SCOPUS, CINAHL Complete, and Cochrane Central. From 16 selected studies, 15 were single-center; 6 prospective, 9 retrospective; and 1 was a randomized trial comparing OP IV diuresis to oral home regimen. Demographics, visit data, and outcomes were collected, and 30-day outcomes were compared to inpatient IV (IP IV) diuresis from 2021 Medicare HF hospitalizations.ResultsThe review included 1,590 unique patients treated with OP IV diuretics, with a mean age of 70 ± 6 years, 69.7% male, and 74.8% NYHA III-IV. Minimal adverse post-diuresis events such as hypokalemia, hypotension, and worsening renal function occurred (4.5%, 0.7%, and 2.3% respectively). Post-visit mean weight loss was −2.2 ± 1.1 kg. The 30-day readmission rate for OP IV diuresis was significantly lower than IP IV diuresis (20.0% vs. 22.6%; p = 0.0.401), and 30-day mortality was also lower (5.6% vs. 10.7%; p = 0.003).DiscussionOP IV diuresis is a safe and effective treatment for decompensated HF with minimal risk of adverse events. Data demonstrate reduced 30-day readmission, mortality rates, cost. These findings highlight the potential of OP IV diuresis as an enhanced alternative HF care; however, further randomized control trials are needed to evaluate long-term outcomes.
The effect of seaweed fertilisation on sulfur isotope ratios (δ34S) and grain size in barley: implications for agronomy and archaeological research
Authors: Blanz Magdalena , Gröcke Darren R. , Martin Peter , Church Mike J. 
Published: November 2024

IntroductionStable sulfur isotope ratios (δ34S) in bone collagen are often employed to study the consumption of marine and freshwater fish, wetland grazing, marine foraging patterns, and the possible geographic origins of food sources. However, a recent small-scale crop experiment showed that biofertilisation with seaweed can elevate δ34S in Celtic beans by ca. 10 ‰. Consumption of this food could erroneously suggest a marine diet and therefore has important implications for the reconstruction of past diets and dietary origins. However, limited research has so far been undertaken on cereals.MethodsTo address this issue, a large-scale field trial was undertaken on the Orkney Islands, whereby bere barley (a Scottish landrace, Hordeum vulgare L.) was biofertilised with seaweed at different dosages (25 t/ha, 50 t/ha), with a mineral NPK fertiliser, and left unfertilised as a control.ResultsThe total barley biomass yield was higher and barley grains were enlarged following all fertilisation treatments compared to the control barley. Barley grain and straw from seaweed-fertilised crops had more elevated δ34S values by around 2–3 ‰ compared to unfertilised plants, while the NPK-fertilised grains and plants had δ34S values 1 ‰ lower.DiscussionThese results confirm previous hypotheses that seaweed fertilisation can elevate cereal δ34S values. The comparatively small δ34S difference between control and seaweed fertilised crops in this field trial is likely due to background elevated δ34S values in the soil (+12.7 ‰), which in turn may be due to long-term exposure to oceanic-influenced rain and sea spray and/or possible historical application of seaweed, or the underlying bedrock composition. The results of this study show that seaweed fertilisation can increase barley grain sizes and δ34S values, and thus should be considered when reconstructing land management and dietary practices in the archaeological record.
Why not ask them? A systematic scoping review of research on dyadic teacher–student relationships as perceived by students with emotional and behavioral problems
Authors: Vösgen-Nordloh Meike , Kulawiak Pawel R. , Bolz Tijs , Koomen Helma M. Y. , Hennemann Thomas , Leidig Tatjana 
Published: November 2024

IntroductionMeta-analytical findings indicate that high-quality dyadic teacher-student relationships (TSRs) can act as social protective factors against the development or persistence of emotional and behavioral problems (EBPs) by fostering students’ social-emotional and cognitive learning. However, previous research primarily focused on samples of students without EBPs and relied on teacher-rated TSRs. Research on dyadic TSRs from the perspective of students with EBPs is scarce, yet their self-perceptions could offer valuable insights into whether and how dyadic TSRs serve as protective factors for them. Therefore, this systematic scoping review is guided by the central question of how research on dyadic TSRs from the perspective of students with EBPs has been conducted and what insights have been gained to date.MethodsThis question is explored through a narrative synthesis of existing studies, thereby identifying current research approaches, empirical findings, practical implications, and future research needs. A database search using keywords related to EBPs and TSRs yielded 24 included studies.Results and discussionSynthesizing these studies reveals five overarching conclusions. First, students with EBPs and their teachers tend to experience dyadic TSRs less favorably than typically developed (TD) students and their teachers. Second, relationship-enhancing interventions focusing on either teacher-student interactions, contextual factors or individual characteristics of teachers and students appear to be effective for students with EBPs. Third, students with EBPs seem to perceive stronger positive affective relationships with their teachers than teachers do with them. Fourth, students with EBPs may perceive their dyadic TSRs as ambivalent, exhibiting both highly positive and highly negative aspects. Fifth, for the social–emotional and academic development of students with EBPs, dyadic TSR-quality seems to be a risk or protective factor, acting differently than in TD-student. However, due to the diverse nature of the included studies, these conclusions remain only preliminary. Consequently, the review concludes with 10 key recommendations that might guide future research on dyadic TSRs from the perspective of students with EBPs.
IL7R, GZMA and CD8A serve as potential molecular biomarkers for sepsis based on bioinformatics analysis
Authors: Li Jin , Wang Lantao , Yu Bin , Su Jie , Dong Shimin 
Published: November 2024

PurposeSepsis is an unusual systemic reaction to what is sometimes an otherwise ordinary infection, and it probably represents a pattern of response by the immune system to injury. However, the relationship between biomarkers and sepsis remains unclear. This study aimed to find potential molecular biomarkers, which could do some help to patients with sepsis.MethodsThe sepsis dataset GSE28750, GSE57065 was downloaded from the GEO database, and ten patients with or without sepsis from our hospital were admitted for RNA-seq and the differentially expressed genes (DEGs) were screened. The Metascape database was used for functional enrichment analysis and was used to found the differential gene list. Protein-protein interaction network was used and further analyzed by using Cytoscape and STRING. Logistic regression and Correlation analysis were used to find the potential molecular biomarkers.ResultsTaking the intersection of the three datasets yielded 287 differential genes. The enrichment results included Neutrophil degranulation, leukocyte activation, immune effectors process, positive regulation of immune response, regulation of leukocyte activation. The top 10 key genes of PPI connectivity were screened using cytoHubba plugin, which were KLRK1, KLRB1, IL7R, GZMA, CD27, PRF1, CD8A, CD2, IL2RB, and GZMB. All of the hub genes are higher expressed in health group of different databases. Logistic regression showed that IL7R, GZMA and CD8A proteins were analyzed and all of them were statistically significant. Correlation analysis showed that there was a statistically significant correlation between IL7R, GZMA and CD8A.ConclusionKLRK1, KLRB1, IL7R, GZMA, CD27, PRF1, CD8A, CD2, IL2RB, GZMB are key genes in sepsis, which associated with the development of sepsis. However, IL7R, GZMA and CD8A may serve as the attractively potential molecular biomarkers for sepsis.
Characteristics of cytokines/chemokines associated with disease severity and adverse prognosis in COVID-19 patients
Authors: Cheng Jianghao , Wang Haozhen , Li Chaodan , Yu Jianhua , Zhu Mingli 
Published: November 2024

BackgroundCytokines and chemokines as crucial participants in innate immune response play significant roles during SARS-CoV-2 infection, yet excessive immune response exacerbates the severity of COVID-19.PurposeThis study aims to investigate the involvement of which cytokines/chemokines in the cytokine storm of COVID-19, as well as the changes in cytokine/chemokine levels during the course of COVID-19, simultaneously exploring the diagnostic and prognostic value of the relevant cytokines/chemokines for COVID-19.MethodsFlow cytometry was employed to detect the levels of cytokines and chemokines in the serum of 50 COVID-19 patients.ResultsCompared with severe COVID-19 patients, the levels of cytokines IL-6, IL-8, IL-10, sCD25, and chemokines IP-10 and MIG in the peripheral blood of non-severe patients were significantly reduced, while only IL-6, IL-10, and IP-10 levels were significantly decreased compared to non-survivors of COVID-19. Meanwhile, serum concentrations of IP-10, MCP-1, sTREM-1, IL-10, and the neutrophil-to-lymphocyte ratio (NLR) in peripheral blood could distinguish between COVID-19 survivors and non-survivors and were significantly associated with mortality. Among them, the concentration of IP-10 was shown to be the most powerful indicator for predicting adverse outcomes in COVID-19 patients (AUC: 0.715); however, its combined detection with the conventional inflammatory marker NLR did not improve the predictive value for adverse outcomes in COVID-19 patients. Additionally, serum IP-10 levels were negatively correlated with peripheral blood NK cell count and total lymphocyte count, while sTREM-1 levels were positively correlated with peripheral blood CD4+ T cell count and CD3+ T cell count. Meanwhile, IL-8 levels were positively correlated with total lymphocyte count in peripheral blood. Finally, the serum levels of cytokines/chemokines in non-survivors of COVID-19 increased significantly before death, while in survivors, they returned to normal levels before discharge.ConclusionsSeverely ill and non-surviving COVID-19 patients exhibit compromised immune function, with significantly higher levels of inflammation, cytokine/chemokine storms, and immune dysregulation compared to non-severe patients. Serum concentrations of IP-10, MCP-1, sTREM-1, and IL-10 levels can serve as biomarkers to predict adverse outcomes in COVID-19.
Associations between glycan signature alterations and the cellular antigenic properties of passaged chondrocytes
Authors: Homan Kentaro , Tokuhiro Taiki , Onodera Tomohiro , Hanamatsu Hisatoshi , Furukawa Jun-ichi , Ebata Taku , Matsuoka Masatake , Kadoya Ken , Terkawi M. Alaa , Iwasaki Norimasa 
Published: November 2024

BackgroundCartilage repair is a significant clinical challenge because of the limited intrinsic healing capacity. Current therapeutic strategies, such as cell transplantation therapy, aim to overcome this challenge by replacing damaged tissue with healthy cells. However, the long-term survival and functionality of transplanted cells remain major hurdles.ObjectiveThis study investigated the impact of chondrocyte passaging on glycan profiles and their antigenic properties. We hypothesized that alterations in glycan composition due to passaging may contribute to the enhanced ability to activate macrophages, thereby affecting the outcome of cell transplantation therapy.MethodsPeritoneal macrophages and primary articular chondrocytes were isolated from C57BL/6 mice to establish direct and indirect coculture models. Macrophage activation was assessed by measuring the concentrations of IL-6 and nitric oxide in the culture supernatants or their gene expression. Glycome analysis of various glycoconjugates was performed by glycoblotting methods combined with the SALSA procedure for N-glycans and GSLs and the BEP method for O-glycans.ResultsOur results revealed that direct coculture of macrophages with passaged chondrocytes increased the production of proinflammatory cytokines, including IL-6 and NO, as the number of passages increased. With increasing passage number, the expression of GD3 substantially decreased, and the expression of GM3, especially GD1a, significantly increased. Coculturing passaged GM3S knockout chondrocytes with macrophages significantly suppressed IL-6 expression, implying reduced macrophage activation.ConclusionThe observed activation of macrophages due to alterations in the glycan profile of chondrocytes provides a possible explanation for the antigenicity and immune rejection of transplanted cells.
Development of a pan-genotypic monoclonal antibody-based competitive ELISA for the detection of antibodies against Bovine viral diarrhea virus
Authors: Qi Shuhui , Wang Jing , Le Ting , Sun Chao , Chang Jitao , Jiang Zhigang , Yin Xin , Pang Quanhai 
Published: November 2024

IntroductionBovine viral diarrhea virus (BVDV), a positive-sense single-stranded RNA virus, causes significant economic losses in the cattle industry. Current diagnostic methods for BVDV exhibit variable sensitivity and specificity, underscoring the need for more rapid and accurate detection approaches. Here, we developed a novel competitive ELISA (cELISA) to detect antibodies against the BVDV E2 protein.Methods and resultsWe generated three monoclonal antibodies (mAbs)—3E6, 2D5, and 5B9—by immunizing mice with purified BVDV E2 protein expressed in Expi293F cells. Among these, mAb 3E6 displayed superior competitive binding abilities to the E2 protein, enabling effective differentiation between BVDV positive and negative sera. Remarkably, mAb 3E6 exhibited pan-genotypic recognition of various BVDV strains, including BVDV-1a, -1b, -1c, -1m, -1p, -1v, and -2a, while showing no cross-reactivity with the classical swine fever virus (CSFV). Computational modeling using AlphaFold 3 identified domain B of the E2 protein as the primary binding site for mAb 3E6. Building upon these findings, we established a cELISA employing mAb 3E6 and recombinant E2 protein. Receiver-operating characteristic (ROC) analysis revealed outstanding diagnostic performance, achieving a sensitivity of 99.26% and specificity of 98.99%. Further tests confirmed the cELISA's specificity for detecting BVDV-specific antibodies, with no cross-reactivity with antisera from animals infected or immunized against BCoV, BHV-1, BRV, AKAV, LSDV, BLV, and CSFV. Consistency was observed between results from the BVDV E2 cELISA and traditional virus neutralization test (VNT), demonstrating high sensitivity for monitoring antibody dynamics. In performance evaluations, the established cELISA exhibited high concordance with VNT in assessing 160 vaccinated sera and 190 clinical samples.DiscussionThe BVDV E2 cELISA, utilizing mAb 3E6 to target domain B of the BVDV E2 protein, represents a reliable and effective serological diagnostic tool for the detection of antibodies against both BVDV-1 and BVDV-2. This methodology holds significant promise for applications in clinical diagnosis and the evaluation of vaccine efficacy.
Reevaluating 30 cmH2O endotracheal tube cuff pressure: risks of airway mucosal damage during prolonged mechanical ventilation
Authors: Mu Guo , Wang Feixiang , Li Qiang , Yu Xuan , Lu Bin 
Published: November 2024

BackgroundThe optimal endotracheal tube (ETT) cuff pressure remains contentious. In the traditional consideration that the level 30 cmH2O is considered safe, balancing the prevention of reflux aspiration against airway mucosal damage. Whether this pressure level can cause potential damage to the airway mucosa remains to be discussed.MethodsAirway mucosa damage and structural changes at 30 cmH2O were examined in patients under general anesthesia and in rabbit mechanical ventilation models. Prior to this, we also interviewed some anesthesiologists about the level of concern about ETT cuff pressure.ResultsA total of 634 valid questionnaires suggested that anesthesiologists generally do not pay enough attention to ETT cuff pressure and the average established cuff pressure significantly exceeded 30 cmH2O. Airway mucosa images of 100 general anesthesia patients with different ventilation duration indicated that maintaining the pressure at 30 cmH2O did not cause significant damage to airway mucosa in a short period of time, while it still caused damage to airway mucosa in patients with long-term ventilation, with damage severity increasing with longer ventilation periods. This correlated strongly with postoperative sore throat (R2 = 0.3884, p &lt; 0.001). In rabbits, 4 h of ventilation at this pressure resulted in significant loss of ciliated epithelium and inflammation. Calculations suggested an effective dose (ED50) to prevent mucosal injury at a cuff pressure of 25.64 cmH2O (95% CI: 19.268–29.367 cmH2O).ConclusionThe currently established cuff pressure of 30 cmH2O is associated with airway mucosal damage in both clinical and animal models. Lowering the safety threshold of cuff pressure may be necessary to mitigate mucosal injury.
The clinical value of fibrosis indices for predicting the hemorrhagic transformation in patients with acute ischemic stroke after intravenous thrombolysis
Authors: Chen Jiahao , Li Xiaoqin , Hu Rui , Hu Chuanchen 
Published: November 2024

BackgroundThe incidence of stroke in China is approximately 343 per 100,000 people each year, the highest rate worldwide. Hemorrhagic transformation (HT), particularly symptomatic intracerebral hemorrhage (sICH) following acute ischemic stroke (AIS) with or without intravenous thrombolysis (IVT), can lead to rapid neurological deterioration, poor prognosis, and even death. Non-alcoholic fatty liver disease (NAFLD) has been identified as a risk factor for stroke occurrence and associated with poor long-term functional outcomes. Nonetheless, no studies have examined the association between liver fibrosis and HT in AIS patients who underwent IVT.MethodA total of 826 patients with AIS who underwent IVT were included in this study. We calculated nine validated liver fibrosis indices to assess the extent of liver fibrosis. HT was detected by follow-up cranial CT/MRI within 24 h post-IVT and was classified as either hemorrhagic infarction (HI) or parenchymal hematoma (PH). Symptomatic intracranial hemorrhage was defined as a sudden symptomatic neurological deterioration, indicated by an increase in (National Institutes of Health Stroke Scale) NIHSS score of 4 points or more.ResultThe median values of fibrosis-4 (FIB-4), modified FIB-4 (mFIB-4), aspartate aminotransferase (AST)-platelet ratio index (APRI), Forns index, alanine aminotransferase (ALT)/AST (ARR), AST/ALT ratio-platelet ratio index (AARPRI), fibrosis quotient (FibroQ), and Fibrosis Index were significantly higher, while the fibrosis-5 (FIB-5) was significantly lower in the HT and sICH groups (all P &lt; 0.001). After adjusting for potential confounders, all nine liver fibrosis indices remained associated with HT and sICH. Receiver operating characteristic (ROC) curve analysis revealed that the FibroQ score had the best predictive ability for HT (AUC = 0.707, CI = 0.652–0.762, P &lt; 0.001), while FIB-4 had the best predictive ability for sICH (AUC = 0.802, CI = 0.711–0.892, P &lt; 0.001).ConclusionLiver fibrosis, as validated by FIB-4, mFIB-4, FIB-5, APRI, Forns index, ARR, AARPRI, FibroQ, and Fibrosis Index, was associated with HT and sICH in AIS patients after IVT.
The indirect effect of nonadherence on health-related quality of life in older adults with neurological disorders: implications for clinical endpoints and interventions
Authors: Schönenberg Aline , Mendorf Sarah , Prell Tino 
Published: November 2024

ObjectiveThis study assessed how Health-Related Quality of Life (HRQoL) and nonadherence to medication are linked, to determine whether HRQoL is a suitable endpoint for clinical trials evaluating nonadherence.BackgroundHRQoL is often used as an endpoint in clinical trials to determine the effectiveness of nonadherence interventions. However, the relationship between HRQoL and nonadherence is not clear, as some interventions find an effect of nonadherence on HRQoL while others do not. Since both HRQoL and nonadherence are latent constructs, it is of interest to understand the factors that link them.MethodsMedication nonadherence was assessed in 731 older adults with neurological disorders using the Stendal Adherence to Medication Score (SAMS). Regression and network analyses were performed to examine the association between the SAMS and HRQoL (SF-36). Cognitive function, depressive symptoms, mobility, and healthcare satisfaction were included as covariates.ResultsThere was a weak association between the SAMS and HRQoL only for the mental component scale. The relationship between the SAMS and HRQoL appears indirect, as its effect is nullified upon the inclusion of covariates, especially depressive symptoms. Network analyses showed that the effect of nonadherence on HRQoL is mainly delivered by depressive symptoms, while cognition and satisfaction with healthcare contribute to a lesser extent.ConclusionNonadherence and HRQoL are both latent variables influenced by similar factors. The effect of nonadherence on HRQoL seems to be indirect and mainly delivered by depressive symptoms, possibly via motivational pathways. These associations need to be considered when selecting clinical endpoints and planning interventions.