bluuebunny/crossref_metadata_2025_split huggingface dataset:
Expert consensus document: Reporting checklist for quantification of pulmonary congestion by lung ultrasound in heart failure
Authors: Platz Elke , Jhund Pardeep S. , Girerd Nicolas , Pivetta Emanuele , McMurray John J.V. , Peacock W. Frank , Masip Josep , Martin‐Sanchez Francisco Javier , Miró Òscar , Price Susanna , Cullen Louise , Maisel Alan S. , Vrints Christiaan , Cowie Martin R. , DiSomma Salvatore , Bueno Hector , Mebazaa Alexandre , Gualandro Danielle M. , Tavares Mucio , Metra Marco , Coats Andrew J.S. , Ruschitzka Frank , Seferovic Petar M. , Mueller Christian ,   on behalf of the Study Group on Acute Heart Failure of the Acute Cardiovascular Care Association and the Heart Failure Association of the European Society of Cardiology
Published: June 2019

AbstractLung ultrasound is a useful tool for the assessment of patients with both acute and chronic heart failure, but the use of different image acquisition methods, inconsistent reporting of the technique employed and variable quantification of ‘B‐lines,’ have all made it difficult to compare published reports. We therefore need to ensure that future studies utilizing lung ultrasound in the assessment of heart failure adopt a standardized approach to reporting the quantification of pulmonary congestion. Strategies to improve patient care by use of lung ultrasound in the assessment of heart failure have been difficult to develop. In the present document, key aspects of standardization are discussed, including equipment used, number of chest zones assessed, the method of quantifying B‐lines, the presence and timing of additional investigations (e.g. natriuretic peptides and echocardiography) and the impact of therapy. This consensus report includes a checklist to provide standardization in the preparation, review and analysis of manuscripts. This will serve as a guide for investigators and clinicians and enhance the quality and transparency of lung ultrasound research.
On the use of correction factors for the mathematical modeling of insulator based dielectrophoretic devices
Authors: Hill Nicole , Lapizco‐Encinas Blanca H. 
Published: June 2019

AbstractMathematical modeling is a fundamental component in the development of new microfluidics techniques and devices. Modeling allows for the rapid testing of new system configurations while saving resources. Microscale electrokinetic (EK) techniques have significantly benefited by the advances in modeling programs and software packages. However, EK phenomena are complex to model, as they dynamically affect system characteristics, including the physical properties of the particles and fluid within the system. Insulator‐based dielectrophoresis (iDEP) is an EK technique that has received important attention during the last two decades. In particular, numerous research groups that study iDEP systems employ a combination of modeling and experimentation for developing new iDEP systems. An important fraction of these research groups has adopted the practice of employing “correction factors” to account for EK phenomena that cannot be accurately predicted in their models due to model complexity and limitations in computing resources. The present review article aims to provide the reader with an overview of the most common approaches in the use of correction factors for the modeling of iDEP systems.
Near‐fault acceleration pulses and non‐acceleration pulses: Effects on the inelastic displacement ratio
Authors: Chang Zhiwang , De Luca Flavia , Goda Katsuichiro 
Published: June 2019

SummaryNear‐fault ground motions can impose particularly high seismic demands on the structures due to the pulses that are typically observed in the velocity time‐histories. The velocity pulses can be further categorized into either a distinct acceleration pulse (acc‐pulse) or a succession of high‐frequency, one‐sided acceleration spikes (non‐acc‐pulse). The different characteristics of velocity pulses imply different frequency content of the ground motions, potentially causing different seismic effects on the structures. This study aims to investigate the characteristics of the two types of velocity pulses and their impacts on the inelastic displacement ratio (CR) of single‐degree‐of‐freedom systems. First, a new method that enables an automated classification of velocity pulses is used to compile a ground motion dataset which consists of 74 acc‐pulses and 45 non‐acc‐pulses. Several intensity measures characterizing different seismological features are then compared using the two groups of records. Finally, the influences of acc‐pulses and non‐acc‐pulses on the CR spectra are studied; the effects of pulse period and hysteretic behavior are also considered. Results indicate that the characteristics of the two types of velocity pulses differ significantly, resulting in clearly distinct CR spectral properties between acc‐pulses and non‐acc‐pulses. Interestingly, mixing acc‐pulses and non‐acc‐pulses can lead to local “bumps” that were found in the CR spectral shape by previous studies. The findings of this study highlight the importance of distinguishing velocity pulses of different types when selecting near‐fault ground motions for assessing the nonlinear dynamic response of structures.
Latest Permian–early Triassic arc amalgamation of the Eastern Tianshan (NW China): Constraints from detrital zircons and Hf isotopes of Devonian–Triassic sediments
Authors: Chen Zhenyu , Xiao Wenjiao , Windley Brian F. , Schulmann Karel , Mao Qigui , Zhang Zhiyong , Zhang Ji'en , Li Yongchen , Song Shuaihua 
Published: June 2019

The temporal–spatial framework of arc amalgamation is an important key for understanding the anatomy of orogenic collages, present and past. The Junggar‐Balkhash Ocean was a major branch of the southern Paleo‐Asian Ocean where several arcs were amalgamated. Among them, the Dananhu arc with its characteristic juvenile magmatism and lack of Precambrian basement is an efficient recorder of the evolutional history. From our U–Pb and Lu–Hf isotopic analyses of Devonian to Triassic sediments in the Dananhu arc and Permian sediments in the Yamansu‐Central Tianshan arc, we discovered two major changes in detrital zircon provenance of the Dananhu arc: (a) An increasing input of Precambrian ages from the Mongolia collage at 850, 1,850, and 2,500 Ma, together with Phanerozoic zircons ranging from 420 to 480 Ma with negative εHf(t) value since ca. 288 Ma; (b) the presence of the Yamansu‐Central Tianshan arclike Precambrian age cluster of 1,400–1,600 Ma around 243 Ma. In combination with regional tectonothermal events, these two changes correspond to the formation of the Harlik‐Dananhu composite arc in the latest Carboniferous–early Permian followed by its collision with the Yamansu‐Central Tianshan arc in the latest Permian–early Triassic, which marks the termination of the eastern Junggar‐Balkash Ocean. Analysis of the sedimentary successions within the intraoceanic arcs sheds light on the amalgamation history of the Central Asian Orogenic Belt.
On the relevance of EEG resting theta activity for the neurophysiological dynamics underlying motor inhibitory control
Authors: Pscherer Charlotte , Mückschel Moritz , Summerer Lena , Bluschke Annet , Beste Christian 
Published: June 2019

AbstractThe modulation of theta frequency activity plays a major role in inhibitory control processes. However, the relevance of resting theta band activity and of the ability to spontaneously modulate this resting theta activity for neural mechanisms underlying inhibitory control is elusive. Various theoretical conceptions suggest to take these aspects into consideration. In the current study, we examine whether the strength of resting theta band activity or the ability to modulate the resting state theta activity affects response inhibition. We combined EEG‐time frequency decomposition and beamforming in a conflict‐modulated Go/Nogo task. A sample of N = 66 healthy subjects was investigated. We show that the strength of resting state theta activity modulates the effects of conflicts during motor inhibitory control. Especially when resting theta activity was low, conflicts strongly affected response inhibition performance and total theta band activity during Nogo trials. These effects were associated with theta‐related activity differences in the superior (BA7) and inferior parietal cortex (BA40). The results were very specific for total theta band activity since evoked theta activity and measures of intertrial phase coherency (phase‐locking factor) were not affected. The data suggest that the strength of resting state theta activity modulates processing of a theta‐related alarm or surprise signal during inhibitory control. The ability to voluntarily modulate theta band activity did not affect conflict‐modulated inhibitory control. These findings have important implications for approaches aiming to optimize human cognitive control.
Evaluating Noninvasive Markers to Identify Advanced Fibrosis by Liver Biopsy in HBV/HIV Co‐infected Adults
Authors: Sterling Richard K. , King Wendy C. , Wahed Abdus S. , Kleiner David E. , Khalili Mandana , Sulkowski Mark , Chung Raymond T. , Jain Mamta K. , Lisker‐Melman Mauricio , Wong David K. , Ghany Marc G. ,   the HIV‐HBV Cohort Study of the Hepatitis B Research Network
Published: June 2019

Noninvasive biomarkers are used increasingly to assess fibrosis in patients with chronic liver disease. We determined the utility of dual cutoffs for noninvasive biomarkers to exclude and confirm advanced fibrosis in hepatitis B virus (HBV)–human immunodeficiency virus (HIV) co‐infected patients receiving combined antiretroviral therapy. Participants were anti‐HIV/hepatitis B surface antigen–positive adults from eight clinical sites in the United States and Canada of the Hepatitis B Research Network. Fibrosis was staged by a central pathology committee using the Ishak fibrosis score (F). Clinical, laboratory, and vibration‐controlled transient elastography (VCTE) data were collected at each site. Dual cutoffs for three noninvasive biomarkers (aspartate aminotransferase‐to‐platelet ratio index, Fibrosis‐4 index [FIB‐4], and liver stiffness by VCTE) with the best accuracy to exclude or confirm advanced fibrosis (F ≥ 3) were determined using established methodology. Of the 139 enrolled participants, 108 with a liver biopsy and having at least one noninvasive biomarker were included: 22% had advanced fibrosis and 54% had normal alanine aminotransferase. The median (interquartile range) of APRI (n = 106), FIB‐4 (n = 106), and VCTE (n = 63) were 0.34 (0.26‐0.56), 1.35 (0.99‐1.89), and 4.9 (3.8‐6.8) kPa, respectively. The area under the curve for advanced fibrosis was 0.69 for APRI, 0.66 for FIB‐4, and 0.87 for VCTE. VCTE cutoffs of 5.0 kPa or less (to exclude) and 8.8 kPa or greater (to confirm) advanced fibrosis had a sensitivity of 92.3% and specificity of 96.0%, respectively, and accounted for 65.1% of participants. Among the 34.9% with values between the cutoffs, 26.1% had advanced fibrosis. Considering APRI or FIB‐4 jointly with VCTE did not improve the discriminatory capacity. Conclusion: VCTE is a better biomarker of advanced fibrosis compared with APRI or FIB‐4 in HBV/HIV co‐infected adults on combined antiretroviral therapy. Using VCTE dual cutoffs, approximately two‐thirds of patients could avoid biopsy to determine advanced fibrosis.
Renal cell carcinoma treatment during pregnancy: Histopathological findings suggestive of rapid tumor growth
Authors: Ohyama Takehiro , Shimbo Masaki , Endo Fumiyasu , Kyono Yoko , Akitani Fumi , Hayashi Tokuhito , Komatsu Kenji , Matsushita Kazuhito , Suzuki Kosuke , Hattori Kazunori 
Published: June 2019

IntroductionDiagnosis of renal cell carcinoma during pregnancy is rare. We report a case of renal cell carcinoma during pregnancy with rapid growth.Case presentationA 39‐year‐old woman presented to our hospital for treatment of renal tumor at 22 weeks gestation. The tumor had a cystic lesion with a partition and showed rapid growth from 28 mm to 32 mm over a period of 4 weeks. The tumor was diagnosed as renal cell carcinoma and an open partial nephrectomy was scheduled at 26 weeks gestation. The operation and perioperative course were successful. Pathological findings confirmed the tumor to be clear cell renal cell carcinoma with G2 > G3, Fuhrman grade 2, pT1a, negative surgical margin, and positive detection of progesterone receptor.ConclusionWe reported the successful management of a patient who was diagnosed with renal cell carcinoma during pregnancy. We also had a suggested association between rapid growth tumor and progesterone based on histopathological analysis of the tumor.
High‐content analysis boosts identification of the initial cause of triptolide‐induced hepatotoxicity
Authors: Zheng Nan , Wang Tiantian , Wei Aili , Chen Wei , Zhao Changqi , Li Hua , Wang Lili 
Published: June 2019

AbstractTriptolide (TP) has been widely used in China for more than 40 years as an immunosuppressive agent. Recently, serious concerns have been raised over TP‐induced liver injury, though the real hepatotoxic mechanism is still unclear, particularly in terms of the initial cause. To our knowledge, this study is the first to screen systematically the mechanism of TP‐induced toxicity through a global cytotoxicity profile high‐content analysis using three independent cytotoxic assay panels with multiple endpoints of cytotoxicity, including cell loss, mitochondrial membrane potential, nuclear membrane permeability, manganese superoxide dismutase, phosphorylated gamma‐H2AX, light chain 3B, lysosome, reactive oxygen species and glutathione. We assessed nine parameters and four stress response pathway models by labeling nuclear factor erythroid 2‐related factor 2, activating transcription factor 6, hypoxia inducible factor 1α and nuclear factor κB and found that all testing parameters except glutathione and manganese superoxide dismutase showed concentration‐ and time‐dependent changes, as well as increased cell loss after TP treatment. Considering that RNA polymerase II is the molecular target of TP, we quantified transcription from inducible genes, bromodeoxyuridine incorporation, and expression from transiently transfected green fluorescence protein plasmids in HepG2 cells. The results show that inhibition of global transcription by TP took place at earlier times and at lower concentrations than those observed for cell death. Therefore, global transcriptional suppression and the cell dysfunction it drives play a central role in TP‐induced hepatotoxicity. This provides valuable information for the safe use of TP in the clinic.
Pentosidine Is Associated With Cortical Bone Geometry and Insulin Resistance in Otherwise Healthy Children
Authors: Kindler Joseph M , Laing Emma M , Liu Weixi , Dain Joel A , Lewis Richard D 
Published: June 2019

ABSTRACT
Pentosidine is an advanced glycation end product (AGE) associated with fracture in adults with diabetes. AGE accumulation in bone collagen contributes to bone fragility but might also adversely influence bone turnover and, consequently, bone geometry. The relationships between AGEs and bone health have yet to be studied in children. Thus, the objective of this study was to assess relationships between pentosidine and cortical bone volumetric density, geometry, and estimated strength in children. Participants were otherwise healthy black and white boys and girls, ages 9 to 13 years, who were at sexual maturation stage 2 or 3 (N = 160). Tibia and radius cortical bone and muscle area (66% site) were assessed via pQCT. In fasting sera, insulin, glucose, and pentosidine were measured. The Quantitative Insulin Sensitivity Check Index (QUICKI), a measure of insulin sensitivity, was calculated. While controlling for race, sex, maturation, and height, pentosidine negatively correlated with QUICKI (P &lt; 0.05). In unadjusted analyses, pentosidine was associated with lower radius and tibia cortical volumetric bone mineral density, bone mineral content (Ct.BMC), area (Ct.Ar), and thickness (Ct.Th); a larger radius endosteal circumference (Endo.Circ); and lower tibia polar strength strain index (all P &lt; 0.05). While controlling for race, sex, maturation, height, and muscle area, pentosidine was negatively associated with tibia Ct.BMC, Ct.Ar, and Ct.Th but positively associated with Endo.Circ (all P &lt; 0.05). Linear regression revealed a significant interaction between pentosidine and QUICKI in relation to tibia Ct.Th (pinteraction = 0.049), indicating that the negative relationship between pentosidine and Ct.Th was stronger in those with lower QUICKI (ie, greater insulin resistance). This is the first study to report evidence of a potentially adverse influence of AGEs on bone strength in otherwise healthy children. This relationship was strongest in children with the greatest insulin resistance, supporting further work in youth with chronic metabolic health conditions. © 2019 American Society for Bone and Mineral Research.
Enolase1 overexpression regulates the growth of gastric cancer cells and predicts poor survival
Authors: Qiao Hui , Wang Yufeng , Zhu Bingdong , Jiang Lei , Yuan Wenzhen , Zhou Yongning , Guan Quanlin 
Published: June 2019

AbstractGastric cancer has become the third most common cancer around the world. In patients with gastric cancer, the 5‐year survival rate is still low. However, the mechanism underlying gastric cancer remains largely unknown. As a glycolytic enzyme, enolase 1 (ENO1) is widely expressed in most tissues. The functions of ENO1 have been reported in various types of cancer. Here in this study, we identified that ENO1 promoted the growth of gastric cancer cells through diverse mechanisms. Our immunohistochemical, bioinformatic and Western blot data showed that ENO1 was significantly overexpressed in human gastric cancer cell lines and tissues. The survival analysis revealed that ENO1 overexpression predicted poor survival in the patients suffering gastric cancer. Knockdown of ENO1 expression repressed the rate of proliferation and capacity of colony formation in two human gastric cancer cell lines (MGC‐803 and MKN‐45). In addition, knockdown of the expression of ENO1 led to the arrest of the cell cycle at the G1 phase and promoted the apoptosis of MKN‐45 and MGC‐803 cells. The further microarray and bioinformatic analysis revealed that ENO1 regulated the expression of diverse genes, many of which are involved in the progress of cancer. Taken together, our data demonstrated that ENO1 was an oncogene‐like factor and might serve as a promising target for the treatment of human gastric cancer.
miR‐34a‐5p was involved in chronic intermittent hypoxia‐induced autophagy of human coronary artery endothelial cells via Bcl‐2/beclin 1 signal transduction pathway
Authors: Lv Xin , Wang Kexin , Tang Wenwen , Yu Lei , Cao Huan , Chi Weiwei , Wang Baoshan 
Published: June 2019

AbstractAutophagy refers to the genetically regulated process to regulate the survival and death of cells, which is conserved in evolution. Typically, autophagy exerts a vital part under physiopathological conditions. Whether autophagy can be resulted from chronic intermittent hypoxia (CIH), a prominent characteristic of obstructive sleep apnea‐hypopnea syndrome (OSAHS), remains to be investigated. Furthermore, microRNAs (miRNAs) can serve as the regulating factors in a variety of benign and malignant diseases; nonetheless, it remains to be fully illustrated about the way by which miRNAs modulate autophagy. According to our results, for human coronary artery endothelial cells (HCAECs), CIH increased the expression of autophagy‐associated proteins, which depended on the concentration and time; besides, it could promote autophagic vacuole (AV) formation. In addition, CIH could activate beclin 1, which was dependent on dose and time. In HCAECs, microRNA‐34a‐5p (miR‐34a‐5p) was overexpressed after exposed to CIH, and its target protein B‐cell lymphoma 2 (Bcl‐2) was downregulated. Moreover, inhibiting miR‐34a‐5p increased Bcl‐2 and p62 expression, while downregulating beclin 1, Vps34, Atg5, and LC3 levels, implying the role of miR‐34a‐5p in CIH‐induced autophagy. Moreover, exogenous upregulation of Bcl‐2 could block miR‐34a‐5p influence on CIH‐induced autophagy through suppressing beclin 1 expression. Additionally, beclin 1 could enhance the autophagy induced by CIH. In conclusion, overexpression of miR‐34a‐5p activated beclin 1 through Bcl‐2 inhibition in CIH and participated in CIH‐induced autophagy.
Elucidation of the mechanism underlying CD44v6‐induced transformation of IEC‐6 normal intestinal epithelial cells
Authors: Chung Shin‐Yi , Huang Wen‐Chen , Chen Zong‐Siang , Chao Ta‐Chung , Su Yeu 
Published: June 2019

AbstractThe transformation abilities of CD44s and CD44v6 in normal intestinal epithelial cells have not yet been reported. Herein, we established both CD44s and CD44v6 overexpressing stable clones from rat IEC‐6 cells and demonstrated that the CD44v6 clones had higher saturation density and anchorage independence. Additionally, CD44v6 clones were more resistant to oxaliplatin and irinotecan which might be attributed to a significantly increased B‐cell lymphoma 2 level and a reduced DNA damage response in these cells. Moreover, c‐Met and vascular endothelial growth factor receptor 2 signalings were involved in modulating the saturation density in CD44v6 clones. Interestingly, higher activation of both AKT and extracellular‐signal‐regulated kinase (ERK) were detected in CD44v6 clones which might account in part for the cell density‐independent nuclear localization of Yes‐associated protein (YAP). To no surprise, increases of both saturation density and anchorage independence in CD44v6 clones were markedly diminished by PI3K, AKT, MEK, and ERK inhibitors as well as YAP knockdown. By contrast, overexpression of a constitutively active YAP robustly increased the aforementioned phenotypes in IEC‐6 cells. Collectively, our results suggest that upregulation of CD44v6, but not CD44s, induces the transformation of normal intestinal epithelial cells possibly via activating the c‐Met/AKT/YAP pathway which might also explain the important role of CD44v6 in the initiation of various carcinomas.
Assessment of niobium phosphate as heterogeneous catalyst in esterification with methyl acetate
Authors: Alves Matheus AL , Pinheiro Nadini SC , Brondani Leoni N , Celante Dian , Ketzer Felipe , Castilhos Fernanda 
Published: June 2019

AbstractBACKGROUNDThe aim of this work was to investigate the esterification reaction from oleic acid with methyl acetate (MeA) catalyzed by niobium phosphate to produce methyl esters. This catalyst has an amorphous structure and large surface area. Catalytic tests were performed according to an experimental design. The results were analyzed statistically and all of the variables were significant, especially the temperature.RESULTSA kinetic study of the reaction was carried out and the most suitable reaction conditions were 240 °C, 10% (m/m) of catalyst and MeA: oleic acid molar ratio 10:1. After 2 h, it was possible to obtain ≈80% (m/m) methyl oleate content. A noncatalytic test corroborated niobium phosphate activity in the reaction. A kinetic model fitted experimental data for the catalyzed and noncatalyzed reactions; kinetics parameters for Arrhenius equation were estimated. In a reuse test, the catalyst showed a gradual decrease in catalytic activity over five reaction cycles.CONCLUSIONNiobium phosphate can be considered as a promising heterogeneous catalyst for biodiesel synthesis. © 2019 Society of Chemical Industry