bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mesenchymal stem cell therapies for intervertebral disc degeneration: Consideration of the degenerate niche
Authors: Vickers Louise , Thorpe Abbey A. , Snuggs Joseph , Sammon Christopher , Le Maitre Christine L. 
Published: May 2019

AbstractWe have previously reported a synthetic Laponite crosslinked poly N‐isopropylacrylamide‐co‐N, N′‐dimethylacrylamide (NPgel) hydrogel, which induces nucleus pulposus (NP) cell differentiation of human mesenchymal stem cells (hMSCs) without the need for additional growth factors. Furthermore NP gel supports integration following injection into the disc and restores mechanical function to the disc. However, translation of this treatment strategy into clinical application is dependent on the survival and differentiation of hMSC to the correct cell phenotype within the degenerate intervertebral disc (IVD). Here, we investigated the viability and differentiation of hMSCs within NP gel within a catabolic microenvironment. hMSCs were encapsulated in NPgel and cultured for 4 weeks under hypoxia (5% O2) with ± calcium, interleukin‐1β (IL‐1β), and tumor necrosis factor alpha (TNFα) either individually or in combination to mimic the degenerate environment. Cell viability and cellular phenotype were investigated. Stem cell viability was maintained within hydrogel systems for the 4 weeks investigated under all degenerate conditions. NP matrix markers: Agg and Col II and NP phenotypic markers: HIF‐1α, FOXF1, and PAX1 were expressed within the NPgel cultures and expression was not affected by culture within degenerate conditions. Alizarin red staining demonstrated increased calcium deposition under cultures containing CaCl2 indicating calcification of the matrix. Interestingly matrix metalloproteinases (MMPs), ADAMTS 4, and Col I expression by hMSCs cultured in NPgel was upregulated by calcium but not by proinflammatory cytokines IL‐1β and TNFα. Importantly IL‐1β and TNFα, regarded as key contributors to disc degeneration, were not shown to affect the NP cell differentiation of mesenchymal stem cells (MSCs) in the NPgel. In agreement with our previous findings, NPgel alone was sufficient to induce NP cell differentiation of MSCs, with expression of both aggrecan and collagen type II, under both standard and degenerate culture conditions; thus could provide a therapeutic option for the repair of the NP during IVD degeneration.
Ecological engineering projects increased vegetation cover, production, and biomass in semiarid and subhumid Northern China
Authors: Niu Quanfu , Xiao Xiangming , Zhang Yao , Qin Yuanwei , Dang Xinghai , Wang Jie , Zou Zhenhua , Doughty Russell B. , Brandt Martin , Tong Xiaowei , Horion Stephanie , Fensholt Rasmus , Chen Chi , Myneni Ranga B. , Xu Weiheng , Di Guangzhi , Zhou Xiaoming 
Published: May 2019

AbstractMultiple ecological engineering projects have been implemented in semiarid and subhumid Northern China since 1978 with the purpose to combat desertification, control dust storms, and improve vegetation cover. Although a plethora of local studies exist, the effectiveness of these projects has not been studied in a systematic and comprehensive way. Here, we used multiple satellite‐based time‐series data as well as breakpoint analysis to assess shifts in leaf area index (a proxy for green vegetation cover), gross primary production, and aboveground biomass in Northern China. We documented increased vegetation growth in northwest and southeastern parts of the region, despite drought anomalies as documented by the standardized precipitation‐evapotranspiration index during 1982–2016. Significant breakpoints in leaf area index were observed for over 72.5% of the southeastern and northwestern regions, and 70.6% of these breakpoints were detected after 1999, which correspond well to the areas with the highest ecological engineering efforts. Areas with negative trends were mainly located in the Inner Mongolian Plateau, Hulun Biur, Horqin Sand Land, and urban areas. The Loess Plateau had the largest increase in vegetation growth, followed by the north parts of Northern China where biomass increased more in the provinces of Shanxi, Liaoning, Shannxi, Hebei, and Beijing than Xinjiang, Inner Mongolia, Tianjin, and Qinghai. Our results show that multiple ecological engineering projects in the region have increased vegetation cover, production, and aboveground biomass that have led to improved environmental conditions in the study area.
Distinct response patterns of soil bacteria to oxalate imply their role in buffering soil acidification: Evidence from red soils with long‐term fertilisation regimes
Authors: Xu Qicheng , Li Kaisong , Ruan Yang , Kong Yali , Liu Manqiang , Ling Ning , Shen Qirong 
Published: May 2019

AbstractLong‐term chemical‐only fertilisation (NPK) decreases soil pH, but this soil acidification is rarely reported with manure fertilisation (M). Soil acidification always leads to a negative effect on sustainable development. As oxalate oxidation is accompanied by soil alkalisation, it is still unknown how and to what extent bacterial oxalate oxidation in soils with different fertilisation regimes contributes to buffering of soil acidification. To assess the potential role of oxalotrophy in buffering soil acidification, two soils that underwent contrasted fertilisation regimes for over 30 years were used. The oxalotrophic communities were characterised based on a biomarker of oxalotrophic taxa (the frc gene), and their responsiveness to calcium oxalate (CaOx) addition was assessed. In addition, stable isotope probing was employed to explore the active bacteria responding to CaOx addition. Despite similar abundances of oxalotrophic bacteria between NPK and M soils before microcosm incubation, the M soil harboured more oxalotrophic bacteria belonging to Deltaproteobacteria, and the NPK soil was colonised by more oxalotrophic bacteria affiliated with Actinobacteria, Alphaproteobacteria, and Betaproteobacteria. In the incubation experiments, the M soil took half of the time that the NPK soil needed to deplete the added CaOx. The faster CaOx consumption rate could be explained by the higher functional redundancy and higher proportion of fast‐response oxalotrophic taxa in M soil. The most dominant active oxalotrophic taxa were Oxalicibacterium and Burkholderia in the M and NPK soils, respectively. Furthermore, the better performance of oxalate oxidation was conjectured to contribute to a higher soil acidification buffering ability. This study obtained an important cue to the feasibility of active oxalotrophic taxa in buffering acidification in soils with different fertilisation regimes, which provides valuable evidence for exploring alternative ways for alleviating soil acidification.
Plant‐mycorrhizae association affects plant diversity, biomass, and soil nutrients along temporal gradients of natural restoration after farmland abandonment in the Loess Plateau, China
Authors: Liu Hongfei , Liang Chutao , Ai Zemin , Zhang Jiaoyang , Wu Yang , Xu Hongwei , Xue Sha , Liu Guobin 
Published: May 2019

AbstractThe interaction between plants and arbuscular mycorrhizal fungi in the rhizosphere plays a vital role in driving vegetation recovery and restoration of soil nutrients. However, how this interaction affects vegetation succession and how soil nutrient recovery is driven by vegetation restoration and rhizosphere processes are still largely unknown. In this study, a well‐documented grassland restoration chronosequence on the Loess Plateau, China (fields at 0, 7, 12, 17, 22, and 32 years after farmland abandonment and a natural grassland reference) was selected. The species richness and diversity reached maximum values between 17 and 22 years after farmland abandonment, whereas the plant total above and belowground biomasses simultaneously peaked at 22 years and then remained stable. In the dominant plant rhizosphere and bulk soil concentrations of total glomalin‐related soil protein (including both old and recently produced fungal proteins) substantially increased from 3.58 to 4.87 g kg−1 and from 2.67 to 3.86 g kg−1, respectively, between 12 and 32 years after farmland abandonment. The concentrations of soil organic carbon (SOC) and total nitrogen (TN) in the plant rhizosphere and bulk soil significantly increased between 17 and 32 years and reached the levels of the natural grassland. The aboveground plant biomass, soil SOC, and TN concentrations were positively correlated with the glomalin‐related soil protein (GRSP) concentration (p < .05). Our study suggested that interactions among plant‐mycorrhizae association, plant diversity, and biomass promote GRSP and nutrient accumulation in the plant rhizosphere and bulk soil, and GRSP largely contributes to SOC stabilization and the accumulation of SOC and TN.
Nitrogen transformations differentially affect nutrient‐limited primary production in lakes of varying trophic state
Authors: Scott J. Thad , McCarthy Mark J. , Paerl Hans W. 
Published: May 2019

AbstractThe concept of lakes “evolving” phosphorus (P) limitation has persisted in limnology despite limited direct evidence. Here, we developed a simple model to broadly characterize nitrogen (N) surpluses and deficits, relative to P, in lakes and compared the magnitude of this imbalance to estimates of N gains and losses through biological N transformations. The model suggested that approximately half of oligotrophic lakes in the U.S.A. had a stoichiometric N deficit, but 72–89% of eutrophic and hypereutrophic lakes, respectively, had a similar N deficit. Although reactive N appeared to accumulate in the most oligotrophic lakes, net denitrification perpetuated the N deficit in more productive lakes. Productive lakes exported reactive N via biological N transformations regardless of their N deficit. The lack of N accumulation through N fixation underscores the need for a modern eutrophication management approach focused on reducing total external nutrient loads, including both N and P.
Recent Advances in the Design of Ionenes: Toward Convergence with High‐Performance Polymers
Authors: Bara Jason E. , O'Harra Kathryn E. 
Published: May 2019

AbstractIonenes, condensation polymers wherein the charge (typically cationic) lies directly within the polymer backbone, have been known for over 85 years. Historically, ionenes have been synthesized from 3odiamines and α,ω‐dihaloalkanes, forming chains of ammonium cations tethered by flexible hydrocarbon linkages with “free” halide anions. However, the requisite building blocks of ionenes are by no means limited only to such molecules. In recent years, ionenes with more sophisticated backbone chemistries have been produced, with a trend toward the use of functionalities associated with classical “high‐performance” condensation polymers such as polyimides and polyarylamides. The expansion of ionenes is also catalyzed by the rapid growth of research in imidazolium‐based ionic liquids (ILs), wherein the combination of ionenes with ILs can yield unexpected behaviors. Furthermore, when considering the largely unexplored experimental space in anionic ionenes, the opportunities for new materials are virtually endless. This review primarily focuses on developments in ionenes published in the scientific literature since 2010, but also includes some older examples that may not have received sufficient attention at the time of their original publication yet can provide some key lessons for the future of ionene design.
The interaction effect of mobile phone screen and product orientation on perceived product size
Authors: Schmidt Lennard , Maier Erik 
Published: May 2019

AbstractPerceived product size is a key concern in online retail, particularly in fashion and grocery. The screen on which consumers view a product (e.g., desktop or mobile) might constitute a frame that biases size perception, on the basis of assimilation and contrast effects (pool and store theory). The rise of mobile commerce exacerbates this issue, as framing effects might be stronger versus desktop settings as screens are smaller. Further, as mobile phone's screen orientation varies situationally (vertical vs. horizontal), the perceived product size might vary, depending on the interaction of screen and product orientation. By introducing the framing ratio as a means to predict extent, dimensionality and symmetry of size biases, we generalize specific findings from extant research. Empirically, four experimental studies demonstrate that contextual frames (i.e., vertical vs. horizontal screens) and product orientation (e.g., jeans vs. shoes) interact to bias the size perception, in that sizes are overestimated on the dimension that approaches the frame (high framing ratio), compared with conditions where the frame is distant (low framing ratio). If product size is misperceived, willingness to pay might be affected (e.g., for groceries). Thus, size perceptions have a direct impact on managerially relevant variables.
Biochemical and clinical response after umbilical cord blood transplant in a boy with early childhood‐onset beta‐mannosidosis
Authors: Lund Troy C. , Miller Weston P. , Eisengart Julie B. , Simmons Katrina , Pollard Laura , Renaud Deborah L. , Wenger David A. , Patterson Marc C. , Orchard Paul J 
Published: May 2019

AbstractBackgroundDeficiency in the enzyme β‐mannosidase was described over three decades ago. Although rare in occurrence, the presentation of childhood‐onset β‐mannosidase deficiency consists of hypotonia in the newborn period followed by global development delay, behavior problems, and intellectual disability. No effective pharmacologic treatments have been available.MethodsWe report 2‐year outcomes following the first umbilical cord blood transplant in a 4‐year‐old boy with early childhood‐onset disease.ResultsWe show restoration of leukocyte β‐mannosidase activity which remained normal at 2 years posttransplant, and a simultaneous increase in plasma β‐mannosidase activity and dramatic decrease in urine‐free oligosaccharides were also observed. MRI of the brain remained stable. Neurocognitive evaluation revealed test point gains, although the magnitude of improvement was less than expected for age, causing lower IQ scores that represent a wider developmental gap between the patient and unaffected peers.ConclusionOur findings suggest that hematopoietic cell transplant can correct the biochemical defect in β‐mannosidosis, although preservation of the neurocognitive trajectory may be a challenge.
Effect quantification and value prediction of factors in noninvasive detection for specific fetal copy number variants by semiconductor sequencing
Authors: Zhang Chunhua , Liang Bo , Qiao Longwei , Xuan Liming , Li Hong , He Quanze , Wu Xiaojuan , Lu Jiafeng , Yu Bin , Wang Ting 
Published: May 2019

AbstractBackgroundThe detection limit of noninvasive prenatal testing (NIPT) by next generation sequencing for any given fetal copy number variants (CNV) can be influenced by several factors. In this study, we quantified the effects and predicted the value of parameters for CNVs detection by NIPT.MethodsGenomic DNA from patient's leucocytes with 3.16 Mb microdeletion in 22q11.21 was mixed with DNA from aborted fetal tissues without CNV at various concentrations by an enzyme digestion method. Abnormal DNA at 0% served as negative control. Sequencing of mixture samples (at 0%, 4%, 12%, and 20%) by Ion Proton Sequencer was performed at flow 500, with WISECONDOR as the pipeline in CNV‐calling and bin of 500, 750 and 1,000 kb for counting unique reads. The parameters were evaluated with Box–Behnken design. The region with Z score ≦−3 was marked as a potential microdeletion.ResultsThe equation of Z score depending on fetal fraction, unique read number and bin size was obtained by Box–Behnken design. The negative effect was quantified as the coefficient in the equation. The smallest values of these parameters were defined as 4 M unique read number, and 10.08% fetal DNA concentration at bin of 750 kb for detecting subchromosomal microdeletion of 3.16 Mb.ConclusionThe quantification of effect and value of parameters as well as the method used in this study can benefit the establishment of quality standards for CNVs detection and interpretation of CNVs detection results.
Contralateral hemi‐fifth‐lumbar nerve transfer for unilateral lower limb dysfunction due to incomplete traumatic spinal cord injury: A report of two cases
Authors: Yu Bao‐Fu , Qiu Yan‐Qun , Du Mao‐Xin , Yin Hua‐Wei , Shen Jun , Ye Xuan , Cai Ze‐Yu , Xu Wen‐Dong 
Published: May 2019

AbstractCurrent strategies for the chronic stage of spinal cord injury (SCI) had seen little progress. In this report, we present the use of contralateral L5 nerve transfer for the treatment of incomplete SCI patients with unilateral lower limb dysfunction in two male patients. One was diagnosed with L2 vertebral fracture and dislocation combined with coni medullaris injury 10 months prior, and the other was diagnosed with T6 and T7 vertebral fractures with SCI 24 months prior. The patients were treated with decompression surgery within 24 hr after injury. The patients reached a recovery plateau after 6–8 months of spontaneous recovery of locomotion and sustained paralysis in the right leg and were left confined to the wheelchair. The score on the lower‐extremity Fugl‐Meyer assessment (FMA‐LE) was 7 for both patients. The patients were then enrolled, and they underwent half of the anterior root of the contralateral L5 transfer to S1 and S2 to improve lower limb motor function. A posterior approach was performed to expose the L5, S1, and S2 nerve roots. Half of the anterior root of the left L5 was cut, and end‐to‐end neurorrhaphy from the left L5 to the right S1 and S2 was performed subdurally. After the surgery, routine rehabilitation treatments were prescribed. Muscle strength decreased transiently in the donor‐side before recovering within 12 months postoperatively. Muscle strength was significantly improved on the affected side 2 years postoperatively, when the FMA‐LE scores increased to 14 and 15, respectively. The patients regained independent walking ability with crutches. This report suggests that contralateral hemi‐5th‐lumbar nerve transfer is safe and can benefit incomplete SCI patients with unilateral lower limb dysfunction.
Visual Performance in the “Real World”: Contrast Sensitivity, Visual Acuity, and Effects of Macular Carotenoids
Authors: Roark Mark W. , Stringham James M. 
Published: May 2019

AbstractVisual acuity (VA) is compared to contrast sensitivity (CS) testing in assessing “real‐world” visual performance, and it is recommended that both should be measured routinely in the clinic. The role of nutritional intervention in improving visual performance is reviewed and emphasized. A brief history and illustration of both VA and CS, within the scope of visual performance, is presented. Parameters for effective CS testing in the clinic, and guidelines for interpretation of results, including a new model for understanding the visual impact of changes in CS, are also presented. Relevant research that supports the use of the macular carotenoids lutein, zeaxanthin, and meso‐zeaxanthin to enhance visual performance is reviewed with suggested guidelines for supplementation. CS testing is easily performed at a single intermediate target size and is an excellent tool for the accurate assessment of a patient's overall visual experience. Research continues to uncover the strong link between nutrition and visual performance; the macular carotenoids appear to be especially effective in this regard, and their benefits to visual performance now importantly include contrast sensitivity. Clinicians can provide an improved level of care by incorporating into the examination protocol CS testing and, where appropriate, nutritional counseling and intervention.
Blueberry Supplementation Mitigates Altered Brain Plasticity and Behavior after Traumatic Brain Injury in Rats
Authors: Krishna Gokul , Ying Zhe , Gomez‐Pinilla Fernando 
Published: May 2019

ScopeTraumatic brain injury (TBI) compromises neuronal function required for hippocampal synaptic plasticity and cognitive function. Despite the high consumption of blueberries, information about its effects on brain plasticity and function under conditions of brain trauma is limited. The efficacy of dietary blueberry (BB) supplementation to mitigate the effects of TBI on plasticity markers and associated behavioral function in a rodent model of concussive injury are assessed.Methods and resultsRats were maintained on a diet supplemented with blueberry (BB, 5% w/w) for 2 weeks after TBI. It is found that BB supplementation mitigated a loss of spatial learning and memory performance after TBI, and reduced the effects of TBI on anxiety‐like behavior. BB supplementation prevents a reduction of molecules associated with the brain‐derived neurotrophic factor (BDNF) system action on learning and memory such as cyclic‐AMP response element binding factor (CREB), calcium/calmodulin‐dependent protein kinase II (CaMKII). In addition, BB supplementation reverses an increase of the lipid peroxidation byproduct 4‐hydroxy‐nonenal (4‐HNE) after TBI. Importantly, synaptic and neuronal signaling regulators change in proportion with the memory performance, suggesting an association between plasticity markers and behavior.ConclusionData herein indicate that BB supplementation has a beneficial effect in mitigating the acute aspects of the TBI pathology.
A continuous‐discontinuous model for crack branching
Authors: Tamayo‐Mas Elena , Feliu‐Fabà Jordi , Casado‐Antolin Montserrat , Rodríguez‐Ferran Antonio 
Published: May 2019

SummaryA new continuous‐discontinuous model for fracture that accounts for crack branching in a natural manner is presented. It combines a gradient‐enhanced damage model based on nonlocal displacements to describe diffuse cracks and the extended finite element method (X‐FEM) for sharp cracks. Its most distinct feature is a global crack tracking strategy based on the geometrical notion of medial axis: the sharp crack propagates following the direction dictated by the medial axis of a damage isoline. This means that, if the damage field branches, the medial axis automatically detects this bifurcation, and a branching sharp crack is thus easily obtained. In contrast to other existing models, no special crack‐tip criteria are required to trigger branching. Complex crack patterns may also be described with this approach, since the X‐FEM enrichment of the displacement field can be recursively applied by adding one extra term at each branching event. The proposed approach is also equipped with a crack‐fluid pressure, a relevant feature in applications such as hydraulic fracturing or leakage‐related events. The capabilities of the model to handle propagation and branching of cracks are illustrated by means of different two‐dimensional numerical examples.