bluuebunny/crossref_metadata_2025_split huggingface dataset:
PO-027 Effects of exercise-induced miRNAs on mitochondrial autophagy signaling pathway in PI3K/Akt/mTOR
Authors: Meng Chang 
Published: October 2018

Objective To explain the regulation of mitochondrial autophagy by exercise-induced miRNAs, it provides a new reference for the prevention and treatment of diseases such as inflammation and tumor.
Methods Through the literature data method, this paper refers to more than 100 articles and writes this review, which provides relevant research directions for relevant researchers.
Results Exercise promotes the up-regulation of miR-30, miR-223 and miR-210 expression through the PI3K /Akt / mTOR signaling pathway to inhibit its related target proteins, and promote mitochondrial autophagy to affect inflammation, tumor and other diseases.
Conclusions In recent years, people's concept of exercise has undergone a slow and fundamental change. In addition to medical factors, the role of exercise factors in the prevention or treatment of various diseases such as inflammation and cancer has gradually strengthened. Mitochondrial autophagy has a significant effect on tumor cells. The successful discovery of PI3K/Akt/mTOR signaling pathway provides more support for the involvement of tumor-like diseases. miRNAs may regulate PI3K/Akt/mTOR signaling in mitochondrial autophagy. Pathways significantly affect tumor-like diseases. Exercise is widely recommended as a means of preventing and treating various diseases such as inflammation and tumor. The induction of normoxic exercise and hypoxic exercise has a significant effect on the regulation of PI3K/Akt/mTOR signaling pathway in mitochondrial autophagy by miRNAs. In the future, studies on the regulation of mitochondrial autophagy by miRNAs can focus on the relationship with exercise. At present, the research on mitochondrial autophagy regulated by exercise-induced miRNAs is still insufficient. As a new discovery, it will receive more and more attention. miRNAs regulate the regulation of PI3K/Akt/mTOR signaling pathway in mitochondrial autophagy, which provides a new idea for solving the treatment problems of inflammation, tumor and other diseases.
PO-029 Advances in research on hypoxia-mediated miRNAs affecting glucose metabolism
Authors: Jiang Han 
Published: October 2018

Objective This article reviews the role of miRNAs in promoting insulin sensitivity, controlling insulin synthesis, and regulating insulin resistance in hypoxia exercise, and discusses the relationship between miRNAs and glucose metabolism, and the mechanism of hypoxia-induced regulation of miRNAs in glucose metabolism. It provides a theoretical basis for better research on the prevention and treatment of disorders of glucose metabolism.
Methods This paper uses the literature data method, collects a large number of documents and cites more than 90 articles for comprehensive statistical analysis to write this article, which provides researchers with relevant research directions.
Results Studies have shown that miRNAs such as miR-138, miR-26b, miR-27a, miR-122, miR-802 and miR-143 have regulatory effects on obesity; some miRNAs such as miR-128, miR-7, miR-25, miR -92a, miR-375 and miR-15 family (miR-15a and miR-15b) and other miRNAs play an important role in regulating glycolipid metabolism, thereby maintaining a stable state of glycolipid metabolism. Expressions such as miR-802 and miR-143 are up-regulated in the liver of obese patients, resulting in impaired glucose tolerance.
Conclusions By regulating the expression of target genes and maintaining the homeostasis of glucose metabolism, miRNAs can effectively improve or prevent obesity and disorders of glucose metabolism such as type 2 diabetes. Current studies have shown that miRNAs affect glucose metabolism from insulin sensitivity, insulin synthesis and insulin resistance. At the same time, studies have shown that exercise intervention can effectively improve glucose metabolism. However, the research on the metabolism of miRNAs in glucose metabolism during hypoxia is still insufficient. It is for further study. Studying the mechanism of the effects of miRNAs on glucose metabolism in hypoxic exercise can not only provide a theoretical basis for scientific hypoglycemic and weight control, but also can be used as an intervention for the prevention and control of diseases related to glucose metabolism disorders. In the future, drugs can regulate the expression of miRNAs, thereby providing a new therapeutic approach for the treatment of diseases caused by abnormal glucose and lipid metabolism.
PO-032 The protective effects of curcumin on exercise-induced renal ischemia reperfusion injury in rats
Authors: Li Fang , Cao Jianmin , Zhou Haitao , Niu Yanlong 
Published: October 2018

Objective This study was designed to investigate the effects of curcumin on inflammatory factors and ECM expression in exercise-induced renal ischemia reperfusion injury in rats.
Methods Sixty 7-week-old male SD rats were divided randomly into three groups: group A (normal control group, n=12), group B (overtraining group, n=24) and group C (curcumin + overtraining group, n=24). Group B and C performed 6 weeks of incremental load training on the treadmill. 24 hours after the last training, the rats were anesthetized intraperitoneally, the morphology of renal tissue and the deposition of glomerular ECM were observed using light microscope，the related biochemical indexes were tested.
Results (1) the renal structure of rats in group A were normal, histopathological changes were observed in group B and C, Paller score of group B were significantly higher than group A(P <0.01), and that of group C were significantly lower than group B(P <0.05).
(2) Blood urea nitrogen (BUN) and serum creatinine (Scr), serum and renal inflammatory factors , TGF-β protein expression level and glomerular ECM deposition of group B were significantly higher than group A(P<0.01) and those of group C were lower than group B(P<0.05).
Conclusions Supplementation of curcumin can effectively protect rats from exercise-induced renal ischemia reperfusion injury, by inhibiting the up-regulation of inflammatory cytokines and TGF-β expression and maintaining the dynamic balance of ECM .
PO-122 Influence of Winter Training on Physiological and Biochemical Indexes of Bicyclist
Authors: Lv Yihao 
Published: October 2018

Objective The research took the bicyclists in Zhejiang for preparing of the Thirteenth National Games in winter training as the subjects. Through physiological and biochemical index of testing during the winter training, we attempted to find out some rules or problems by exploring the characteristics of their training during winter training, Reached on body function and the training intensity of athletes for effective monitoring and adjustment effect during winter training, and to provide a reference for the development of scientific in bicycle training.
Methods During the long term bicycle training, some physical fitness evaluation indices includingVO2max,anaerobic power,body composition,Hb,Bun,CK,T and NEUT% were tested and analyzed systematically. Master the bike athletes body function changes during the winter training, and discuss the relationship between these indexes and body function condition.
Results VO2 max, VO2 max relative value almost no change during the winter training; The Large Anaerobic work, 30s average anaerobic work and an body composition indicators no significant difference (p < 0.05), but had a significant change. Hb, Bun, CK,T,NEUT% based standard value in the normal range, throughout the winter training period, Hb, BUN and CK, T, NEUT % has experienced cyclical changes along with the change of sports load: Hb, T at the beginning of the winter training has a downward trend, CK and NEUT % in the early part of the winter training has a rising trend, but as for the winter training gradually restored, BUN rising during the winter training, two weeks before the end of winter training to get down.
Conclusions 1, the pros and cons of aerobic capacity is not the main factors influencing the cycling short athlete special performance, but of eliminating anaerobic lactic acid after training to play an active role, aerobic ability training should be strengthened.
2, anaerobic metabolism energy is the important factors that affect cycling short athlete sport result, peak power and 30s average power to scientific evaluation of the athlete's training effect.
3, decreased body fat percentage and lean body mass increase, to improve the athlete special scores play a positive role.
4, during the winter training athletes Hb, CK, T, NEUT % changes over motion load, NEUT % can be used as auxiliary training monitoring indexes of female athletes on the biochemical indexes of the sensitivity is higher than male athletes.
5, in the case of lean body mass is not fall, blood urea to maintain at a high level of cycling short athletes may not bring much impact performance.
6, Because of the mobilization can bear the load and strength are not the same, the same load and intensity produced by the reflect function will not the same, further analysis is needed to combined with physiological and biochemical indexes and individual training plan situation carries on the comprehensive consideration, so as to improve the accuracy of measurement analysis.
PO-131 Impact of short-term inhibition of PKA in Nucleus Accumbens on voluntary wheel running
Authors: Mao Xuansong , Grigsby Kolter , Booth Frank 
Published: October 2018

Objective Based upon a Booth lab goal of establishing molecular regulators of physical activity motivation, my current study focuses on the effects of short-term inhibition of protein kinase A (PKA) activity in the nucleus accumbens (NAc). The NAc is a brain region integral to motivated behaviors. Downstream immediate-early gene (IEG) expression from PKA has been shown to exhibit rapid responses to acute stimuli, such as voluntary wheel-running behavior. According to previous work in our lab, long-term NAc overexpression of the endogenous PKA inhibitor, Protein Kinase Inhibitor Alpha (PKIα), increased nightly running distance in rats selectively bred for low voluntary running (LVR) behavior (Mol Neurobiol 2018 Jun 21). However, paradoxically, the same PKIα overexpression failed to increase running distance in wild-type (WT) rats. It is known that chronic manipulation of the NAc PKA pathway produces different molecular (gene expression profiles) and behavioral outcomes from that of acute manipulations. Given the above, the goal of the current work is to determine how short-term inhibition of PKA in the NAc influences its downstream gene networks and the nightly voluntary running behavior in WT rats.
Methods An ex vivo preparation of the NAc was utilized to determine the effects of Rp-cAMPS, a selective protein kinase A inhibitor, upon its stimulation of dopamine D1-like receptor agonist SKF 38393 on downstream gene expression level in sedentary WT female rats. Further, real-time PCR was implemented to analyze the transcriptional expression of IEGs (Homer-1, Arc, Zif268) following Rp-cAMPS administration.
Results Data showed that there were no significant difference of mRNA level for Homer-1, Arc or Zif268 among the vehicle, 50uM, 100uM and 200uM Rp-cAMPS treatment groups upon the stimulation of 10uM SKF 38393.
Conclusions In addition to the PKA, other protein kinases such as Ca++ activated and growth factor activated kinases have both been shown to phosphorylate CREB at Ser133, and thus, lead to activation of gene transcription. Given the above results of the ex vivo experiment, in which NAc slices were treated with multiple dosages of Rp-cAMPS concurrent with the stimulation of SKF 38393, it is possible that other protein kinase pathways could be compensating the effects of short-term inhibition of PKA and, in turn, lead to no difference of IEG expression. Further experiments will need to be performed in order to testify this hypothesis.
PO-134 Optogenetics Active SNc DA Neurons Improve Locomotor Activity on Exercise-Induced fatigue Rats
Authors: Li Ke , Chen MengJiao , Li JiaXin , Zhao XuDong , Qiao Decai , Hou LiJuan 
Published: October 2018

Objective After optogenetics activing SNc DANs, locomotor activity ability of exercise-induced fatigue rats were recorded to research the role of nigra-striatum DA system in central mechanism of motor control.
Methods Used male SD rats (220~240g), randomly divided into 6 groups: sham control group (SCG), optogenetics control group (OCG), fatigue group (FG), sham fatigue group (SFG) and optogenetics fatigue group (OFG). Virus ware injected in the right SNc at coordinates (AP:-5.30 mm, R: 2.00 mm, H-8.00 mm). The SCG/SFG injected 1 μ l saline, OCG/OFG injected 1 μl mixed virus(ChR2 with TH Cre,1:1), the fiber implantation site was deeper than the virus injection about 1 mm. Three weeks after surgery, rats attend 7D exhaustive treadmill exercise. After fatigue running, OCG/OFG/SCG/SFG were in the open field. We used two different light delivery schedules: 10 ms pulses at 20 Hz (10s) and 10 ms pulses at 3 Hz (10s). Sampling time were quiet state, 1D exhaustion, 7D exhaustion and recovery 24h. Animals were euthanized after completion of the behavioral tests. Brains were sectioned coronally in 50-μm slices, images were taken using a fluorescence microscope and determined the anatomical location of the optical fiber.
Results (1) OCG/OFG obtain expression of ChR2 in DANs, this was not observed in SCG/SFG; Activation of OCG DANs with 20Hz increased the normalized mean global activity (laser on/laser off), and a significant increment in normalized mean global activity (laser on/laser off) when DANs were activated with 3Hz/20Hz in OFG (p<0.05), showing successful transfection of OCG/OFG. (2) Stimulation at 20 Hz was sufficient to improve global activity, the effects of 3 Hz did not significant, showing 20Hz stimulation possibility be related to motor regulation. (3)The global activity and total distance of rats after 7D exhaustion ware significantly lower than of quiet state (p<0.05), the difference from the quiet state and after 24h recovery is not significant, indicating that the decline in locomotor ability caused by exercise induced-fatigue is reversible. (4) The 20 Hz stimulation significantly improved the global activity of 1D and 7D exhausted rats (p<0.05), indicating that 20 Hz stimulation can effectively improve the rats locomotor activity with exercise-induced fatigue compared with 3 Hz stimulation.
Conclusions (1) ChR2 virus transfection can effectively active the nigra-striatum DA system, causing changes in motor performance and increasing the locomotor ability of rats; (2) The locomotor ability of rats with exercise-induced fatigue to decrease. The total distance and global activity decreased significantly with exercise-induced fatigue; (3) Photoactivation of SNc DANs can improve the locomotor activity of rats with exercise-induced fatigue. From the perspective of stimulating effect, 20Hz is the most obvious activation reference point (NSFC: 31401018, SKXJX: 2014014, Corresponding houlj@bnu.edu.cn).
PO-138 Mechanism of Cortical Information Output after Exercise Fatigue Induced by D2DR
Authors: Wang Xiaoxin , Li Ke , Hou Lijuan 
Published: October 2018

Objective In this experiment, the Local field potential (LFPs) was observed in the substantia nigra compact and electrical activity change in corticostriatal pathway after D2DR intervention in exercise-induced fatigue rats. We analyzed the changes of DA neuron discharge and D2DR mediated corticostriatal pathway information transmission. To explore the mechanism of D2D2 mediated DA system in the information output of cortical M1 region.
Methods Wistar rats were used to establish the model of exercise-induced fatigue. The rats were divided into control group (CG), 7 days fatigue group (7FG) and 24 hour recovery group (24RG). We used in vivo multichannel recording technology to record electrical activity in the M1, striatum and substantia nigra compact of rats and observed the electrophysiological changes after D2DR intervention. We also detected the expression of TH proteins in the dorsolateral striatum before and after exercise-induced fatigue by immunohistochemistry.
Results 1) Compared with group CG, the expression of TH protein in the dorsolateral area of striatum was significantly decreased in group 7FG (P<0.05). 2) Compared with the CG group, the power spectral density of the θ, α and β band of the SNc was increased after seven days of exhaustion exercise(P < 0.05); After 24 hours of recovery, the PSD value decreased significantly compared with the 7FG group(P<0.05). 3)Compared with the CG group the power spectral density of alpha (7-13Hz) and beta (15-30Hz) bands in the M1 region and striatum was increased in 7FG after injection D2DR agonist(P < 0.05) .
Conclusions After exercise-induced fatigue, the activity of substantia nigra was increased, and the activity of M1 and striatum was lower than that of the blank control group after the D2DR agonist injection. As a key receptor for the DA signal system, D2DR regulates the electrical activity of the nigrostriatal DA pathway and affects the comprehensive information output of the cortex, which can be regarded as a target for exercise-induced fatigue (NSFC: 31401018, SKXJX: 2014014, Corresponding houlj@bnu.edu.cn).
PO-142 Effects of hypoxia preconditioning on acute hypoxic exercise-induced phosphorylation of AMPKα in mice skeletal muscle
Authors: Wei Hao , Wang Lin jia , Zhang Ying 
Published: October 2018

Objective AMP-activated protein kinase (AMPK) is a metabolic energy sensor and its activation plays an important role in the regulation of energy homeostasis. Increasing evidence indicates that AMPK activation depend on the phosphorylation sites in AMPKα. Thr172 is involved in AMPK activation, whereas Ser485/491 are not. Under suitable stress stimulations, the phosphorylation of AMPKα at the Thr172 site can increase AMPK activation. However, serious hypoxic exercise or taking antioxidants before exercise can reduce the activation of AMPK by phosphorylating AMPK α1Ser485/α2Ser491 sites. The aim of this study was to investigate the effects of hypoxia preconditioning on exhaustive exercise under hypoxic condition induced AMPKα Thr172 and Ser485/491 phosphorylation in mice skeletal muscle.
Methods The 40 eight-week-old male C57BL/6J wild type mice were randomly divided into four groups (10 mice /group): non-hypoxia preconditioning control group (NC), hypoxia preconditioning control group (HC), non-hypoxia preconditioning acute hypoxic exercise group (NE), and hypoxia preconditioning acute hypoxic exercise group (HE). Hypoxia preconditioning groups were exposure in hypoxia for 48h, with the oxygen concentration was 11.2%. Meanwhile, non-hypoxia preconditioning was in the normoxic condition for 48h. After hypoxia preconditioning, acute hypoxic exercise groups finished an exhaustive exercise. Tibialis anterior muscles of mice were collected immediately after the exhaustive exercise. The protein expression of the total AMPKα, Thr172-AMPKα phosphorylation, and Ser485-AMPKα1/Ser491-AMPKα2 phosphorylation were measured by Western Blot. Thr172-AMPKα phosphorylation to total AMPKα ratio and Ser485-AMPKα1/Ser491-AMPKα2 phosphorylation to total AMPKα ratio was calculated.
Results Compared with NE group, The Thr172-AMPKα phosphorylation to total AMPKα ratio was increased significantly, whereas the relative expression of Ser485-AMPKα1/Ser491-AMPKα2 phosphorylation to total AMPKα ratio seemed to decreased in skeletal muscle of HE group.
Conclusions The 48h hypoxia preconditioning could improve the AMPK activation by Thr172-AMPKα phosphorylation in mice skeletal muscle following an exhaustive exercise under the hypoxic condition.
PO-145 Effects of HIF-1α on Nrf2-ARE antioxidant signal in mice skeletal muscle after acute exhaustive exercise
Authors: Wang Linjia , Li Tieying , Zhang Ying 
Published: October 2018

Objective Hypoxia or exercise could lead to oxidative stress. Hypoxia inducible factor-1 (HIF-1) is an oxygen sensor and the expression of its α subunit can be regulated by hypoxia. NF-E2-related factor 2 (Nrf2) is an important modifier of cellular responses to oxidative stress. A major mechanism in defense oxidative stress is the activation of the Nrf2-ARE antioxidant pathway. But whether the increase of HIF-1α could affect the Nrf2-ARE antioxidant signal, and further influence the oxidative stress status in vivo remains unknown. In this study, we wished to examine the effect of HIF-1α on Nrf2-ARE antioxidant pathway in mice skeletal muscle after acute exhaustion exercise.
Methods HIF-1α high expression (H) and C57BL/6J mice(W) were used at 20 respectively and each kind of mice were randomly divided into two groups: control (C) and exercise (E). The treadmill exercise was preformed at the acute exhaustion exercise. On the day of acute exercise, mice allocated to perform treadmill running were subject to 5% incline and 5min at 10m/min, and then increased 3m/min every 3 minutes. Mice were sacrificed at the indicated time points following treadmill running.Nrf2, phosphor-Nrf2 (Ser40), nuclear Nrf2 protein were measured by Western Blot and Nrf2-ARE binding activity, the mRNA and proetin levels of Nrf2 target genes, key antioxidant enzymes (SOD1, SOD2, CAT, NQO-1) and ROS level, were also measured in skeletal muscles after the interventions.
Results (1)The results showed that compared with WC, RNA and protein expression level of Nrf2 were increased in HC skeletal muscles. Nrf2-ARE binding activity, Nrf2 target gene SOD1, SOD2, NQO-1 mRNA expression and NQO-1 protein expression were also increased in HC skeletal muscles. Meanwhile, ROS level in HC skeletal muscles decreased significantly.
(2) After the acute exhaustion exercise, high HIF-1α expression mice (HE) had higher expression of p-Nrf2(Ser 40) and nuclear Nrf2 protein than the wide type mice(WE). The mRNA expression of SOD1 and mRNA /protein of NQO-1 in HE increased as well. In contrast, ROS level decreased significantly in HE muscles.
ConclusionsThe result indicated the proper high expression of HIF-1α could promote the antioxidant capacity of skeletal muscle in mice through Nrf2-ARE pathway.