bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rosmarinic Acid Restores Complete Transparency of Sonicated Human Cataract Ex Vivo and Delays Cataract Formation In Vivo
Authors: Chemerovski-Glikman Marina , Mimouni Michael , Dagan Yarden , Haj Esraa , Vainer Igor , Allon Raviv , Blumenthal Eytan Z. , Adler-Abramovich Lihi , Segal Daniel , Gazit Ehud , Zayit-Soudry Shiri 
Published: June 2018

AbstractCataract, the leading cause of vision impairment worldwide, arises from abnormal aggregation of crystallin lens proteins. Presently, surgical removal is the only therapeutic approach. Recent findings have triggered renewed interest in development of non-surgical treatment alternatives. However, emerging treatments are yet to achieve full and consistent lens clearance. Here, the first ex vivo assay to screen for drug candidates that reduce human lenticular protein aggregation was developed. This assay allowed the identification of two leading compounds as facilitating the restoration of nearly-complete transparency of phacoemulsified cataractous preparation ex vivo. Mechanistic studies demonstrated that both compounds reduce cataract microparticle size and modify their amyloid-like features. In vivo studies confirmed that the lead compound, rosmarinic acid, delays cataract formation and reduces the severity of lens opacification in model rats. Thus, the ex vivo assay may provide an initial platform for broad screening of potential novel therapeutic agents towards pharmacological treatment of cataract.
Management of Low and Intermediate Risk Adult Rhabdomyosarcoma: A Pooled Survival Analysis of 553 Patients
Authors: Elsebaie Maha A. T. , Amgad Mohamed , Elkashash Ahmed , Elgebaly Ahmed Saber , Ashal Gehad Gamal E. l. , Shash Emad , Elsayed Zeinab 
Published: June 2018

AbstractThis is the second-largest retrospective analysis addressing the controversy of whether adult rhabdomyosarcoma (RMS) should be treated with chemotherapy regimens adopted from pediatric RMS protocols or adult soft-tissue sarcoma protocols. A comprehensive database search identified 553 adults with primary non-metastatic RMS. Increasing age, intermediate-risk disease, no chemotherapy use, anthacycline-based and poor chemotherapy response were significant predictors of poor overall and progression-free survival. In contrast, combined cyclophosphamide-based, cyclophosphamide + anthracycline-based, or cyclophosphamide + ifosfamide + anthracycline-based regimens significantly improved outcomes. Intermediate-risk disease was a significant predictor of poor chemotherapy response. Overall survival of clinical group-III patients was significantly improved if they underwent delayed complete resection. Non-parameningeal clinical group-I patients had the best local control, which was not affected by additional adjuvant radiotherapy. This study highlights the superiority of chemotherapy regimens –adapted from pediatric protocols- compared to anthracycline-based regimens. There is lack of data to support the routine use of adjuvant radiotherapy for non-parameningeal group-I patients. Nonetheless, intensive local therapy should be always considered for those at high risk for local recurrence, including intermediate-risk disease, advanced IRS stage, large tumors or narrow surgical margins. Although practically difficult (due to tumor’s rarity), there is a pressing need for high quality randomized controlled trials to provide further guidance.
Nesfatin-1 in the dorsal raphe nucleus influences visceral sensitivity via 5-HT neurons in male maternally separated rats
Authors: Zhang Hui-Ai , Sang Nan , Ge Xian , Huang Qian , Li Xue-Liang , Sha Jie 
Published: June 2018

AbstractNesfatin-1, a satiety molecule processed from nucleobindin2 (NUCB2), is implicated in visceral hypersensitivity in rats and colocalized with 5-hydroxytryptamine (5-HT) in the dorsal raphe nucleus (DRN). Maternal separation (MS) in rats contributes to visceral hypersensitivity via elevated expression of 5-HT in the DRN. Intracerebroventricular injection of nesfatin-1 activates DRN 5-HT neurons. In this study, A model of visceral hypersensitivity was developed by subjecting rats to MS. Colorectal distension was used to detect visceral sensitivity, which was evaluated by abdominal withdrawal reflex (AWR) scores and electromyogram (EMG) magnitude. MS rats exhibited higher AWR scores and EMG magnitude compared with controls. The numbers of nesfatin-1- and tryptophan hydroxylase (TPH, the rate-limiting enzyme for 5-HT synthesis)-positive cells in the DRN were significantly elevated accordingly. Visceral hypersensitivity was significantly alleviated in MS rats treated with intra-DRN administration of anti-nesfatin-1/NUCB2, accompanied by decreased expression of 5-HT and TPH in the DRN, compared with the vehicle-treated group. In contrast, intra-DRN administration of nesfatin-1 into normal adult rats induced visceral hypersensitivity, which correlated with elevated expression of 5-HT and TPH in the DRN. In conclusion, Nesfatin-1 has critical effects on visceral hypersensitivity; the underlying mechanisms might be related to the activation of DRN 5-HT neurons.
A memristive plasticity model of voltage-based STDP suitable for recurrent bidirectional neural networks in the hippocampus
Authors: Diederich Nick , Bartsch Thorsten , Kohlstedt Hermann , Ziegler Martin 
Published: June 2018

AbstractMemristive systems have gained considerable attention in the field of neuromorphic engineering, because they allow the emulation of synaptic functionality in solid state nano-physical systems. In this study, we show that memristive behavior provides a broad working framework for the phenomenological modelling of cellular synaptic mechanisms. In particular, we seek to understand how close a memristive system can account for the biological realism. The basic characteristics of memristive systems, i.e. voltage and memory behavior, are used to derive a voltage-based plasticity rule. We show that this model is suitable to account for a variety of electrophysiology plasticity data. Furthermore, we incorporate the plasticity model into an all-to-all connecting network scheme. Motivated by the auto-associative CA3 network of the hippocampus, we show that the implemented network allows the discrimination and processing of mnemonic pattern information, i.e. the formation of functional bidirectional connections resulting in the formation of local receptive fields. Since the presented plasticity model can be applied to real memristive devices as well, the presented theoretical framework can support both, the design of appropriate memristive devices for neuromorphic computing and the development of complex neuromorphic networks, which account for the specific advantage of memristive devices.
Gene expression profiling of periodontitis-affected gingival tissue by spatial transcriptomics
Authors: Lundmark Anna , Gerasimcik Natalija , Båge Tove , Jemt Anders , Mollbrink Annelie , Salmén Fredrik , Lundeberg Joakim , Yucel-Lindberg Tülay 
Published: June 2018

AbstractPeriodontitis is a highly prevalent chronic inflammatory disease of the periodontium, leading ultimately to tooth loss. In order to characterize the gene expression of periodontitis-affected gingival tissue, we have here simultaneously quantified and localized gene expression in periodontal tissue using spatial transcriptomics, combining RNA sequencing with histological analysis. Our analyses revealed distinct clusters of gene expression, which were identified to correspond to epithelium, inflamed areas of connective tissue, and non-inflamed areas of connective tissue. Moreover, 92 genes were identified as significantly up-regulated in inflamed areas of the gingival connective tissue compared to non-inflamed tissue. Among these, immunoglobulin lambda-like polypeptide 5 (IGLL5), signal sequence receptor subunit 4 (SSR4), marginal zone B and B1 cell specific protein (MZB1), and X-box binding protein 1 (XBP1) were the four most highly up-regulated genes. These genes were also verified as significantly higher expressed in gingival tissue of patients with periodontitis compared to healthy controls, using reverse transcription quantitative polymerase chain reaction. Moreover, the protein expressions of up-regulated genes were verified in gingival biopsies by immunohistochemistry. In summary, in this study, we report distinct gene expression signatures within periodontitis-affected gingival tissue, as well as specific genes that are up-regulated in inflamed areas compared to non-inflamed areas of gingival tissue. The results obtained from this study may add novel information on the genes and cell types contributing to pathogenesis of the chronic inflammatory disease periodontitis.
Finite-size correction scheme for supercell calculations in Dirac-point two-dimensional materials
Authors: Rocha C. G. , Rocha A. R. , Venezuela P. , Garcia J. H. , Ferreira M. S. 
Published: June 2018

AbstractModern electronic structure calculations are predominantly implemented within the super cell representation in which unit cells are periodically arranged in space. Even in the case of non-crystalline materials, defect-embedded unit cells are commonly used to describe doped structures. However, this type of computation becomes prohibitively demanding when convergence rates are sufficiently slow and may require calculations with very large unit cells. Here we show that a hitherto unexplored feature displayed by several 2D materials may be used to achieve convergence in formation- and adsorption-energy calculations with relatively small unit-cell sizes. The generality of our method is illustrated with Density Functional Theory calculations for different 2D hosts doped with different impurities, all of which providing accuracy levels that would otherwise require enormously large unit cells. This approach provides an efficient route to calculating the physical properties of 2D systems in general but is particularly suitable for Dirac-point materials doped with impurities that break their sublattice symmetry.
Chemicals and microbes in bioaerosols from reaction tanks of six wastewater treatment plants: survival factors, generation sources, and mechanisms
Authors: Wang Yanjie , Lan Huachun , Li Lin , Yang Kaixiong , Qu Jiuhui , Liu Junxin 
Published: June 2018

AbstractSampling was conducted from biochemical reaction tanks of six municipal wastewater treatment plants in the Yangtze River and Zhujiang deltas and the Jing-Jin-Ji region to assess their morphology, level, and composition. Morphological observations suggested that particles were scattered amorphously with C, O, and Si as the major elements. Bioaerosols are composed of spatially varying levels of microorganisms and chemicals. As the sampling height increased, the level of the components in the bioaerosols decreased. Wastewater in the biochemical reaction tanks was identified as an important source of bioaerosols using SourceTracker analysis. The aerosolization of film drops produced by bursting of bubbles was the main reason for the generation of bioaerosols. Increasing the aeration rate of water may promote bioaerosol generation. Relative humidity, temperature, wind speed, and solar illumination influenced the survival of bioaerosols. Large particle sedimentation and wind diffusion significantly decreased the atmospheric aerosol concentration. When the sampling point height increased from 0.1 m to 3.0 m, the concentrations of the microorganisms and total suspended particles decreased by 23.71% and 38.74%, respectively. Considerable attention should be paid to the control of total suspended particles and microorganisms in bioaerosols.
Viscoelastic optical nonlocality of low-loss epsilon-near-zero nanofilms
Authors: de Ceglia Domenico , Scalora Michael , Vincenti Maria A. , Campione Salvatore , Kelley Kyle , Runnerstrom Evan L. , Maria Jon-Paul , Keeler Gordon A. , Luk Ting S. 
Published: June 2018

AbstractOptical nonlocalities are elusive and hardly observable in traditional plasmonic materials like noble and alkali metals. Here we report experimental observation of viscoelastic nonlocalities in the infrared optical response of epsilon-near-zero nanofilms made of low-loss doped cadmium-oxide. The nonlocality is detectable thanks to the low damping rate of conduction electrons and the virtual absence of interband transitions at infrared wavelengths. We describe the motion of conduction electrons using a hydrodynamic model for a viscoelastic fluid, and find excellent agreement with experimental results. The electrons’ elasticity blue-shifts the infrared plasmonic resonance associated with the main epsilon-near-zero mode, and triggers the onset of higher-order resonances due to the excitation of electron-pressure modes above the bulk plasma frequency. We also provide evidence of the existence of nonlocal damping, i.e., viscosity, in the motion of optically-excited conduction electrons using a combination of spectroscopic ellipsometry data and predictions based on the viscoelastic hydrodynamic model.
The soluble guanylate cyclase stimulator riociguat reduces fibrogenesis and portal pressure in cirrhotic rats
Authors: Schwabl Philipp , Brusilovskaya Ksenia , Supper Paul , Bauer David , Königshofer Philipp , Riedl Florian , Hayden Hubert , Fuchs Claudia Daniela , Stift Judith , Oberhuber Georg , Aschauer Stefan , Bonderman Diana , Gnad Thorsten , Pfeifer Alexander , Uschner Frank Erhard , Trebicka Jonel , Rohr-Udilova Nataliya , Podesser Bruno Karl , Peck-Radosavljevic Markus , Trauner Michael , Reiberger Thomas 
Published: June 2018

AbstractIn cirrhotic patients, portal hypertension (PHT) deteriorates survival, yet treatment options are limited. A major contributor to increased intrahepatic vasoconstriction in PHT is dysfunctional nitric-oxide signaling. Soluble guanylate cyclase (sGC) is the receptor of nitric-oxide and can be stimulated by riociguat. Riociguat is approved for pulmonary hypertension but has not been studied in liver cirrhosis. In this study we assessed the effects of riociguat on PHT and liver fibrosis in cholestatic (bile duct ligation, BDL) and toxic (carbon-tetrachloride, CCl4) rat models. In cirrhotic livers sGC expression was upregulated. In BDL rats, riociguat reduced liver fibrosis and decreased portal pressure without affecting systemic hemodynamics. In an early BDL disease stage, riociguat decreased bile duct proliferation, improved sinusoidal vascular dysfunction and inhibited angiogenesis. In advanced BDL riociguat exhibited anti-inflammatory effects. In CCl4 rats the beneficial effects of riociguat treatment were less pronounced and confined to an early disease stage. Similarly, in patients with cholestatic cirrhosis and PHT nitrates (that induce sGC activity) decreased portal pressure more effectively than in patients with non-cholestatic etiology. We also found an improvement of transaminases in patients with pulmonary hypertension receiving riociguat. Our findings support the clinical development of sGC stimulators in patients with cirrhotic PHT.
Subjective decision threshold for accurate visual detection performance in rats
Authors: Osako Yuma , Sakurai Yoshio , Hirokawa Junya 
Published: June 2018

AbstractThe dissociation between a subjective-criterion performance and forced performance in a sensory detection can provide critical insights into the neural correlates of sensory awareness. Here, we established a behavioral task for rats to test their spatial-visual cue detection ability, using a two alternative choice task with and without a third choice option where animals get rewards only in the objective absence of a visual cue. In the trials without the third option, spatial choice accuracy decreased from near perfect to near chance levels as the visual cue brightness decreased. In contrast, with the third option, the rats exhibited >90% spatial choice accuracy regardless of the cue brightness. The rats chose the third choice option less frequently when the cue was brighter, suggesting that rats have a generalized strategy to make spatial choices only when their internal detection criterion is met. Interestingly, even when the animals chose the third option, they could still significantly and correctly choose the direction of the visual stimuli if they were forced. Our data suggest that the rats’ variable detection performance with identical set of stimuli is derived from stochastic processing of visual signals with a certain internal detection threshold rather than general motivational threshold.
Jagged-1 induced molecular alterations in HPV associated invasive squamous cell and adenocarcinoma of the human uterine cervix
Authors: Tripathi Richa , Rath Gayatri , Hussain Showket , Jawanjal Poonam , Bandil Kapil , Sharma Vishwas , Bharadwaj Mausumi , Mehrotra Ravi 
Published: June 2018

AbstractThe majority of cervical cancer (CC) cases are attributable to HPV infection. Altered Notch pathway signals and HPV are believed to modify clinicopathogenesis of CC, however, the involvement of each molecular player and its mechanism is still not known. Jagged-1 (JAG1) is one of the ligands that induce Notch pathway. The involvement of JAG1 in the modulation of a disease condition is not very clear. Hence, this study aims to study the role of JAG1 in HPV-16/18 associated different histological sub-types of CC, especially ADC. 40 non-neoplastic cervical tissues, 30 precancer and 118 tumor specimens (total 188 tissue biopsies) were studied for the expression of the JAG1 protein through immunohistochemistry, immunoblotting and for HPV infection. Two folds increase of cytoplasmic (Mean ± S.E, 3.67 ± 0.33; p = 0.0001) and nuclear (3.70 ± 0.38, p = 0.0001) JAG1 expression was identified in normal (N) vs precancer and three folds cytoplasmic (4.44 ± 0.17, p = 0.0001) and nuclear (4.64 ± 0.17; p = 0.0001) in N vs. ISCC. Total 85% of ADC patients were found to be infected with HPV, which were 100% infected with HPV-16. These findings suggest the complex synergistic interplay between JAG1 and HPV in regulating clinicopathological progression of CC through its deregulation.
Dynamic features of water molecules in superconcentrated aqueous electrolytes
Authors: Han Sungho 
Published: June 2018

AbstractAn existence of ions dissolved in water has significant effects on bulk properties of water. Superconcentrated conditions have been recently proposed to provide a new concept of lithium ion batteries in order to overcome limitations for practical applications. In those conditions, water would undergo significant changes in structure and dynamics compared to its bulk properties. However, little is known about water in superconcentrated aqueous electrolytes. Here we study the properties of water in aqueous electrolytes with various salt concentrations via molecular dynamics simulations. We find that new dynamic features of water arise in the limit of an extremely high salt concentration. In particular, we observe a decoupled temporal character of water molecules exhibiting a subdiffusive translation and a diffusive rotation in the superconcentrated condition. Furthermore, we find that the rotational dynamics for each principal axis of a water molecule differently responds to the salt concentration, resulting in an occurrence of anisotropy in the rotation as the salt concentration increases. The superconcentrated environments also invoke new features in the hydrogen-bonding characteristics of water such as an emergence of two time scales in the hydrogen bond dynamics of water with respect to the salt concentration.
Influenza viral vectors expressing two kinds of HA proteins for bivalent vaccines against clade 2.3.4.4 and clade 2.3.2.1 H5 HPAIVs
Authors: Hou Guangyu , Li Jinping , Wang Yan , Wang Suchun , Peng Cheng , Yu Xiaohui , Jin Jihui , Jiang Wenming 
Published: June 2018

AbstractThe H5 highly pathogenic avian influenza viruses (HPAIVs) in China pose a serious challenge to public health and the poultry industry. In this study, we constructed a replication-competent recombinant influenza A virus of clade 2.3.4.4 Н5N1 expressing the clade 2.3.2.1 H5 HA1 protein from a tricistronic NS segment. We used a truncated NS1 protein of 73 amino acids combined with a heterologous dimerization domain to increase protein stability. H5 HA1 and nuclear export information were fused in frame with a truncated NS1 open reading frame, separated by 2A self-processing sites. The resulting PR8-H5-NS1(73)H5 stably expressed clade 2.3.4.4 H5 HA and clade 2.3.2.1 H5 HA1 proteins and exhibited similar in vitro growth kinetics as the parental PR8-2344H5 virus. PR8-H5-NS1(73)H5 induced specific hemagglutination-inhibition (HI) antibody against clade 2.3.4.4 H5 that was comparable to that of the combination vaccine of PR8-2344H5 and PR8-2321H5. HI antibody titers were significantly lower against clade 2.3.2.1 H5 virus than with the combination vaccine. PR8-H5-NS1(73)H5 completely protected chickens from both clade 2.3.4.4 and clade 2.3.2.1 H5 HPAIVs challenge. Our results suggested that PR8-H5-NS1(73)H5 was highly immunogenic and efficacious against both clade 2.3.4.4 and clade 2.3.2.1 H5 HPAIVs in chickens.
High‐reflection optical thin films based on SiO2/TiO2 nanoparticles multilayers by dip coating
Authors: Yuehui Wang , Xing Yang 
Published: June 2018

The alternately stacking layers of SiO2 and TiO2 nanoparticles with different refractive indices were fabricated to form the distributed Bragg reflectors on the silicon wafer by dip coating method. By appropriate design of the thickness of the quarter‐wave layers, the peak reflectance region can be tuned from the blue‐green to the infrared portion of the electromagnetic spectrum. The peak reflectance of 65% at 800 nm and at 1600 nm has been achieved using seven periods, respectively; meanwhile, compared with the simulation, 80% at 800 nm and 70% at 1620 nm for seven periods, respectively. The scanning electron microscopy and the atomic force microscope studies confirm the thickness uniformity achieved along the fabrication direction, and a good quality of surfaces and interfaces.
L’horloge circadienne à l’heure Nobel
Authors: Klarsfeld André , Birman Serge , Rouyer François 
Published: June 2018

L’attribution du prix Nobel 2017 de physiologie ou médecine à trois chercheurs américains - Jeffrey C. Hall (né le 3 mai 1945 à New York – University of Maine), Michael Rosbash (né le 7 mars 1944 à Kansas City - Brandeis University, Waltham et Howard Hughes Medical Institute) et Michael W. Young (né le 28 mars 1949 à Miami - Rockefeller University, New York), est difficilement contestable, tant ces chercheurs incarnent depuis près de 35 ans, l’émergence, puis le foisonnement des études moléculaires et cellulaires des rythmes circadiens. Mais ce prix a fait bien plus que trois heureux. Il apporte, en effet, une reconnaissance éclatante à un domaine, la chronobiologie, qui a longtemps fait figure, au mieux pour certains, d’aimable curiosité… La difficulté à identifier les rouages des horloges biologiques qui rythment nos jours et nos nuits, ou même à seulement les imaginer, y a bien sûr contribué. C’est pourquoi les travaux de Hall, Rosbash et Young – récompensés « pour leurs découvertes des mécanismes moléculaires qui contrôlent les rythmes circadiens » – ont revêtu une telle importance, même si la voie leur avait été ouverte un peu plus d’une décennie auparavant. Paradoxalement, le grand public a peut-être admis l’existence de nos horloges internes avant la communauté scientifique, car chacun peut faire l’expérience intime de rythmes journaliers, à commencer par l’alternance veille-sommeil, qui s’imposent à lui !