bluuebunny/crossref_metadata_2025_split huggingface dataset:
Heart Disease in a Mutant Mouse Model of Spontaneous Eosinophilic Myocarditis Maps to Three Loci
Authors: Zimmermann Nives , Gibbons William J. , Homan Shelli M. , Prows Daniel R 
Published: August 2019

Abstract
Background

Heart disease (HD) is the major cause of morbidity and mortality in patients with hypereosinophilic diseases. Due to a lack of adequate animal models, our understanding of the pathophysiology of eosinophil-mediated diseases with heart complications is limited. We have discovered a mouse mutant, now maintained on an A/J inbred background, that spontaneously develops hypereosinophilia in multiple organs. Cellular infiltration into the heart causes an eosinophilic myocarditis, with affected mice of the mutant line (i.e., A/JHD) demonstrating extensive myocardial damage and remodeling that leads to HD and premature death, usually by 15-weeks old.

Results

Maintaining the A/JHD line for many generations established that the HD trait was heritable and implied the mode of inheritance was not too complex. Backcross and intercross populations generated from mating A/JHD males with females from four different inbred strains produced recombinant populations with highly variable rates of affected offspring, ranging from none in C57BL/6J intercrosses, to a few mice with HD using 129S1/SvImJ intercrosses and C57BL/6J backcrosses, but nearly 8% of intercrosses and >17% of backcrosses from SJL-related populations developed HD. Linkage analyses of these SJL/J derived recombinants identified three highly significant loci: a recessive locus mapping to distal chromosome 5 (LOD=4.88; named Emhd1 for eosinophilic myocarditis to heart disease-1); and two dominant variants mapping to chromosome 17, one (Emhd2; LOD=7.51) proximal to the major histocompatibility complex, and a second (Emhd3; LOD=6.89) that includes the major histocompatibility region. Haplotype analysis identified the specific crossovers that defined the Emhd1 (2.65Mb), Emhd2 (8.46Mb) and Emhd3 (14.59Mb) intervals.

Conclusions

These results indicate the HD trait in this mutant mouse model of eosinophilic myocarditis is oligogenic with reduced penetrance, due to multiple segregating variants and possibly additional genetic or nongenetic factors. The A/JHD mouse model represents a unique and valuable tool to understand the interplay of causal factors that underlie the pathology of this newly discovered eosinophil-associated disease with cardiac complications.
Social media interventions for mental health: A scoping review
Authors: Ali Nashva 
Published: August 2019

Abstract
Background: Social media in healthcare has been widely used over the past few years with increasing popularity worldwide. Social media platforms can potentially act as a possible mental health service provider by improving communication, awareness and education. Objective: The aim of this scoping review is to describe the use of social media interventions for mental health and to provide an understanding of the current state of knowledge. Methods: The search platforms utilized for the scoping review were PubMed and IEEE. The inclusion criteria were as follows: research-based articles, primary means for mental health service is social media, and articles that specify mental disorders (depression, anxiety, etc). Results: After applying the inclusion and exclusion criteria on 190 articles, 30 were included for the purpose of this review. Twelve studies originated from the USA while six studies were from the UK. While fourteen papers were on general mental health disorders, twelve papers focused on depression. The results generally showed that social media interventions for mental health had advantages over the conventional treatments. A lack of studies from developing countries, and fewer studies including the elderly population and the assumption of digital literacy are among the identified gaps. Conclusion: Social media has the potential to act as a service provider for users with mental health issues. However, the effectiveness of this method has not been accurately measured and more research is required for evaluating the effectiveness of using social media for treatment over conventional methods.
Systematic review of penile prosthesis infection and meta-analysis of diabetes mellitus role
Authors: Gon Lucas Mira , de Campos Caio Cesar Citatini , Voris Brunno Raphael Iamashita , Pásseri Luís Augusto , Fregonesi Adriano , Riccetto Cássio Luís Zanetini 
Published: August 2019

Abstract
Background: Infection is the most feared complication of penile prosthesis. Diabetes mellitus is a well known to increase risk of several kinds of infection, but its role upon penile prosthesis is not defined. The aim of this systematic review is to show the contemporary scenario of penile prosthesis infection and perform a meta-analysis about diabetes mellitus contribution to penile prosthesis infection. Methods: the review was performed with no language or time limitation, in 10 data bases. There were included articles about male population who received a penile prosthesis with no model restriction, with a minimum follow up of one year and proper outcomes report. Results: The mean incidence of infection after penile prosthesis implant ranged from 0.33 to 11.4%. In early 2000, after several improvements, the general incidence of infection was 3 to 5%. Then, the introduction of coated materials decreased infection to 0.3 to 2.7% . Also, no touch technique and centers of reference with high volume surgeons contributed to reduce infection rates. Meta-analysis showed that diabetes mellitus increases penile prosthesis infection with odds ratio of 1.56 (95% CI: 1.12 - 2.044). Conclusions: Penile prosthesis infection decreased in the last decades, but remains an important cause of reoperation and it is related to lower prosthesis survival. Meta-analysis concludes that diabetes mellitus increases penile prosthesis infection and it should be considered as a risk factor.
Analysis of erroneous data entries in paper based and electronic data collection
Authors: Ley Benedikt , Rijal Komal Raj , Marfurt Jutta , Adhikari Nabaraj , Banjara Megha , Shrestha Upendra Thapa , Thriemer Kamala , Price Ric N. , Ghimire Prakash 
Published: August 2019

Abstract
Objective: Electronic data collection (EDC) has become a suitable alternative to paper based data collection (PBDC) in biomedical research even in resource poor settings. During a survey in Nepal, data were collected using both systems and data entry errors compared between both methods.
Collected data were checked for completeness, values outside of realistic ranges, internal logic and date variables for reasonable time frames. Variables were grouped into 5 categories and the number of discordant entries were compared between both systems, overall and per variable category.
Results: Data from 52 variables collected from 358 participants were available. Discrepancies between both data sets were found in 12.6% of all entries (2352/18,616). Differences between data points were identified in 18.0% (643/3,580) of continuous variables, 15.8% of time variables (113/716), 13.0% of date variables (140/1,074), 12.0% of text variables (86/716), and 10.9% of categorical variables (1,370/12,530). Overall 64% (1,499/2,352) of all discrepancies were due to data omissions, 76.6% (1,148/1,499) of missing entries were among categorical data. Omissions in PBDC (n=1002) were twice as frequent as in EDC (n=497, p<0.001).
Data omissions, specifically among categorical variables were identified as the greatest source of error. If designed accordingly, EDC can address this short fall effectively.
Assessing the prevalence and risk factors of pre-diabetes among the community of Iganga Municipality, Uganda: A cross sectional study
Authors: Aramo Christine , Oyom Anthony Peter , Okello Emmanuel , Acam Victoria , Okiria John Charles , Mwambi Bashir , Oyet Caesar 
Published: August 2019

Abstract
Objective: The prevalence of pre-diabetes is increasing globally with more than 470 million people projected to develop pre-diabetes by 2030. In Africa, the average prevalence of pre-diabetes was estimated at 7.3% in 2015 and affected individual will develop type 2 diabetes mellitus within few decades. The aim of the study was to determine the prevalence of pre-diabetes and associated risk factors among residents of Iganga Municipality. A cross-sectional study was conducted among males and females aged 13-60 years. District health office provided updated household list from which sampling of the villages was performed based on probability proportionate to population. Consented participants were prepared for the study, allowing fasting for 8 to 10 hours before blood collection the next morning. Individuals with impaired fasting glucose, were subjected to OGTT. Results: 130 participants were enrolled, of which 98 were women. The mean age of the participants was 35 years. The prevalence of pre-diabetes was 3.8%. The proportion of impaired glucose tolerance was higher in current smokers (p = 0.01), obese participants (p = 0.002) and hypertensives (p <0.001). Prevalence of pre-diabetes is high in this community and is associated with current smoking, hypertension and high BMI.
Selenium, Copper, Zinc and Hypertension: An Analysis of the National Health and Nutrition Examination Survey (2011-2016)
Authors: Bastola Mrigendra Mani , Locatis Craig , Maisiak Richard , Fontelo Paul 
Published: August 2019

Abstract
Abstract:
Background: Hypertension is a major cardiovascular illness worldwide with many underlying causes. The role of trace elements selenium, copper, and zinc in hypertension is uncertain with only a few studies reported. The objective of this study was to evaluate the role of these trace elements in hypertension.
Method: Data from 6683 National Health and Nutrition Examination Survey (NHANES) participants from 2011 to 2016 were analyzed using Statistical Analytical System (SAS, version 9.4) software for the role of trace elements in hypertension in age range 8 to 80 years, irrespective of the antihypertensive medication taken.
Results: Findings showed a significant positive association between serum selenium levels and hypertension but not with serum copper and zinc. At optimal levels for transport and distribution, serum selenium levels of 120 µg/L or higher (reference level 70 -150 µg/L) were significantly associated with hypertension (OR = 1.56, 95% CI= 1.38 – 1.76) after adjusting for confounding factors. At serum selenium greater than 150 µg/L, the association with hypertension strengthened (OR= 1.91, 95% CI = 1.54-2.36).
Conclusion: A positive association was found between serum selenium and hypertension, irrespective of age or anti-hypertensive medications intake. These findings also suggest that the reference levels of serum selenium levels in healthy individuals may need to be re-determined. If validated, patients with hypertension may also need to be cautioned about selenium intake.
PTPIP51 crosslinks the NFκB signaling and the MAPK pathway in SKBR3 cells and provides a bypass mechanism for NFκB inhibition
Authors: Dietel Eric , Brobeil Alexander , Tag Claudia , Gattenloehner Stefan , Wimmer Monika 
Published: August 2019

Abstract
Background

Previous studies of our group revealed an association of the scaffolding protein Protein Tyrosine Phosphatase Interacting Protein 51 (PTPIP51) with the NFκB signaling on the RelA and IκB level. The NFκB signaling plays a pivotal role in many different tumor entities. Especially HER2 amplified breast cancer cells frequently display activation of the NFκB signaling. Here, the NFκB signaling is linked to the initiation, progression, and the metastasis of the breast cancer. Furthermore, a large body of evidence associates the NFκB signaling with the development of therapy resistance. We aimed to clarify the effects of NFκB inhibition on the NFκB- and the MAPK-related interactome of PTPIP51 in HaCat cells and SKBR3 cells and their correlation with cell viability.

Results

Both NFκB signaling inhibitors, PDTC and IKK-16, reduced the cell viability of SKBR3 cells. IKK-16 selectively reduced the cell viability in SKBR3 cells at 5µM, while the viability of HaCat cells was not affected. PDTC impaired the cell viability of both cell lines and induced a formation of the Raf1/14-3-3/PTPIP51 complex in SKBR3 cells, indicating a shift of PTPIP51 into MAPK signaling. The MAPK-related interactome of PTPIP51 remained unaffected by IKK-16.

Conclusion

(1)The effectiveness and selectivity of NFκB inhibition in malignant and non-malignant signaling systems depends on the level of the targeted signaling molecule. (2) PTPIP51 might serve as the mediator between the NFκB signaling and the MAPK pathway in SKBR3 cells upon NFκB inhibition.
Expansion, Exploitation and Extinction: Niche Construction in Ephemeral Landscapes
Authors: Wetherington Miles , Keymer Juan E. 
Published: August 2019

Abstract
We developed an interacting particle system (IPS) to study the effect of niche construction on metapopulation dynamics in ephemeral landscapes. Using finite scaling theory, we find a divergence in the qualitative behavior at the extinction threshold between analytic (mean field) and numerical (IPS) results when niche construction is confined to a small area in the spatial model. While increasing the area of niche construction leads to a faster rate of range expansion, it also causes a shift from a continuous to discontinuous phase transition, thus returning to the mean field prediction. Furthermore, in the discontinuous regime of the IPS, spatial clustering prior to a critical transition disappears. This is a significant finding as spatial clustering has been considered to be an early warning signal before ecosystems reach their 'tipping point'. In addition to maintaining stability, we find this local niche construction strategy has an advantage when in competition with an exploiter because of their ability to monopolize the constructed niche due to spatial adjacency. However, as the niche construction neighborhood expands this advantage disappears and the exploiter strategy out-competes the niche constructor. In some cases the exploiter pushes the niche constructor to extinction, thus a tragedy of the commons ensues leading to ecological suicide and a collapse of the niche.
Risk factors for parastomal hernia of loop stoma and relationships with other stoma complications in laparoscopic surgery
Authors: Shiraishi Takuya , Nishizawa Yuji , Ikeda Koji , Tsukada Yuichiro , Sasaki Takeshi , Ito Masaaki 
Published: August 2019

Abstract
Purpose: Laparoscopic approach is now a widespread technique used worldwide, but there are few recent studies on risk factors for parastomal hernia. Therefore, this study was performed to analyze the incidence of parastomal hernia in laparoscopic or open loop stoma, and to determine risk factors for parastomal hernia formation. Associations between parastomal hernia and other stoma-related complications were also analyzed.
Methods: A retrospective analysis of patient and surgical characteristics was performed in 153 consecutive cases with stoma creation in loop ileostomy or colostomy performed at our hospital from January to December 2016.
Results: Parastomal hernia developed in 77 cases (50.3%), including 39 (25.5%) diagnosed by physical examination and 38 (24.8%) detected by CT alone. In univariate analysis, a stoma that did not pass through the middle of the rectus abdominis muscle was a significant risk factor for parastomal hernia. This remained as the only independent risk factor for parastomal hernia formation in multivariate analysis. An additional analysis identified a laparoscopic approach as the only independent risk factor for formation of a stoma that did not pass through the middle of the rectus abdominis muscle. An analysis of stoma-related complications showed that peristomal skin disorders were significantly associated with parastomal hernia formation.
Conclusions: This study showed that a stoma that is not formed through the middle of the rectus abdominis muscle is a risk factor for parastomal hernia, and that a laparoscopic approach is associated with this risk factor. Moreover, a parastomal hernia is significantly associated with peristomal skin disorders.
Performance of family planning clinics in conducting recommended HIV counseling and testing in Mombasa County, Kenya: a cross-sectional study
Authors: Eastment McKenna , Wanje George , Richardson Barbra , Nassir Faiza , Mwaringa Emily , Barnabas Ruanne V. , Sherr Kenneth , Mandaliya Kishorchandra , Jaoko Walter , McClelland R. Scott 
Published: August 2019

Abstract
Background

A high proportion of African women utilize family planning (FP) services. Accordingly, incorporating HIV testing into FP services may strategically target the first WHO 90-90-90 goal of 90% of people living with HIV knowing their status.

Methods

The objective of this analysis was to determine the proportion of new FP clients counseled and tested for HIV, as well as correlates of HIV testing, in a random sample of 58 FP clinics in Mombasa County, Kenya. Structured interviews of FP clinic managers collected data on characteristics of FP clinics and staff. Study staff performed a 3-month review of FP registers, summarizing new client HIV testing and counseling (HTC). Because overall rates of HTC were quite low, a binary variable was created comparing clinics performing any HIV counseling and/or testing to clinics performing none. Generalized linear models were used to calculate prevalence ratios (PR) and identify correlates of HTC. Factors associated with any HTC with a p-value <0.10 in univariate analysis were included in a multivariate analysis.

Results

Of the 58 FP clinics, 26 (45%) performed any counseling for HIV testing, and 23 (40%) performed any HIV testing. Counseling for HIV testing was conducted for 815/4389 (19%) new clients, and HIV testing was performed for 420/4389 (10%). Clinics without trained HIV testing providers uniformly did not conduct HIV counseling and/or testing (0/12 [0%]), while 27/46 (59%) of clinics with ≥1 provider performed some HTC (p<0.001). In the subset of 46 clinics with ≥1 trained HIV testing provider, correlates of performing HTC included being a public versus non-public clinic (PR 1.70 95%CI 1.01-2.88), and having an HIV comprehensive care center (CCC) onsite (PR 2.05, 95%CI 1.04-4.06).

Conclusion

Trained HIV testing providers are crucial for FP clinics to perform any HTC. Approaches are needed to increase routine HTC in FP clinics including staffing changes and/or linkages with other testing services (in standalone VCT services or lab facilities) in order to improve the implementation of existing national guidelines. A future cluster randomized trial is planned to test an implementation strategy, the Systems Analysis and Improvement Approach (SAIA) to increase HTC in FP clinics.
Genome-wide miRNA profiling in plasma of pregnant women with Down syndrome fetuses
Authors: Zedníková Iveta , Chylíková Blanka , Šeda Ondřej , Korabečná Marie , Pazourková Eva , Břešťák Miroslav , Krkavcová Miroslava , Calda Pavel , Hořínek Aleš 
Published: August 2019

Abstract
ABSTRACT
Objective:
Down syndrome (DS) belongs to the most common causes of mental retardation in men, therefore new approaches allowing its rapid and effective prenatal detection are being explored. In our study we focused on diagnostic potential of plasma microRNAs. We follow up on our previous study in DS placentas, where seven miRNAs were found as being significantly elevated.
Methods:
A total of 70 first-trimester plasma samples of pregnant women were included to the present study (35 samples with DS fetuses; 35 with euploid fetuses). The genome-wide miRNA profiling was performed in the preliminary study using Affymetrix GeneChip™ miRNA 4.1 Array Strips. Selected miRNAs were then included to the following validation study using qPCR.
Results:
Based on the current pilot study results (12 miRNAs), our previous research on CVS samples (7 miRNAs) and literature (4 miRNAs), the group of 23 miRNAs were selected for validation study. Although the results of the preliminary study were promising, the following validation step using more sensitive technology RT-PCR and larger group of samples revealed no significant differences in miRNA profiles between compared groups.
Conclusion:
Our results suggest that testing of the first trimester plasma miRNAs is not suitable for NIPT. Different results could be theoretically achieved at later gestational ages; however, such a result probably would have limited utilization in clinical practice.
Frequency of trombophilia associated genes. Population – based study.
Authors: Wawrusiewicz-Kurylonek Natalia , Kretowski Adam Jacek , Posmyk Renata 
Published: August 2019

Abstract
Background: Thrombophilia is a hypercoagulable state that may have a genetic basis (inherited) or can be acquired. It is a multifactorial condition and only the mutual interactions between the environment and genes may lead to the development of clinical manifestation. This state is the main factor that promotes venous (rarely arterial) thromboembolism (VTE). The inherited thrombophilia is mainly associated with two pathogenic variants in the V coagulation factor (FV) and the prothrombin (FII) genes. The aim of our study was to evaluate the frequency of two pathogenic variants in FII and FV genes as an inherited thrombophilia factor in a representative group of Polish population in comparison with other described populations.
Methods: All studied groups consisted of 1414 unrelated patients at the age between 18 and 70. The individuals from the research group came from the Podlasie region of Poland. Genotyping of FII, FV and MTHFR variants was performed using the 7900HT Fast Real-Time PCR System and were genotyped by TaqMan assay.
Results: The pathogenic allele frequency for A allele was 0.03 (3%) and 0.07 (7%) for FII and FV genes, respectively. The GA/AA genotypes (c.*97G>A variant) were observed only in 33 (5.03%) individuals in the studied group. Additionally, the frequency of GA/AA genotypes was over 17.4% in the coagulation factor V. Co-instability of heterozygous genotype GA of variants FII and FV genes was observed only in 4 subjects.
Conclusions: Our results is similar to the frequency of the both FII and FV variants in the South–Eastern European countries population, whose value is about 5% and 2.5% respectively.