bluuebunny/crossref_metadata_2025_split huggingface dataset:
Heterogeneous localisation of membrane proteins in Staphylococcus aureus
Authors: Weihs Felix , Wacnik Katarzyna , Turner Robert D. , Culley Siân , Henriques Ricardo , Foster Simon J. 
Published: February 2018

AbstractThe bacterial cytoplasmic membrane is the interface between the cell and its environment, with multiple membrane proteins serving its many functions. However, how these proteins are organised to permit optimal physiological processes is largely unknown. Based on our initial findings that 2 phospholipid biosynthetic enzymes (PlsY and CdsA) localise heterogeneously in the membrane of the bacterium Staphylococcus aureus, we have analysed the localisation of other key membrane proteins. A range of protein fusions were constructed and used in conjunction with quantitative image analysis. Enzymes involved in phospholipid biosynthesis as well as the lipid raft marker FloT exhibited a heterogeneous localisation pattern. However, the secretion associated SecY protein, was more homogeneously distributed in the membrane. A FRET-based system also identified novel colocalisation between phospholipid biosynthesis enzymes and the respiratory protein CydB revealing a likely larger network of partners. PlsY localisation was found to be dose dependent but not to be affected by membrane lipid composition. Disruption of the activity of the essential cell division organiser FtsZ, using the inhibitor PC190723 led to loss of PlsY localisation, revealing a link to cell division and a possible role for FtsZ in functions not strictly associated with septum formation.
The Cue-Approach Task as a General Mechanism for Long-Term Non-Reinforced Behavioral Change
Authors: Salomon Tom , Botvinik-Nezer Rotem , Gutentag Tony , Gera Rani , Iwanir Roni , Tamir Maya , Schonberg Tom 
Published: February 2018

AbstractRecent findings show that preferences for food items can be modified without external reinforcements using the cue-approach task. In the task, the mere association of food item images with a neutral auditory cue and a speeded button press, resulted in enhanced preferences for the associated stimuli. In a series of 10 independent samples with a total of 255 participants, we show for the first time that using this non-reinforced method we can enhance preferences for faces, fractals and affective images, as well as snack foods, using auditory, visual and even aversive cues. This change was highly durable in follow-up sessions performed one to six months after training. Preferences were successfully enhanced for all conditions, except for negative valence items. These findings promote our understanding of non-reinforced change, suggest a boundary condition for the effect and lay the foundation for development of novel applications.
Dynamic control of particle separation in deterministic lateral displacement separator with viscoelastic fluids
Authors: Li Yuke , Zhang Hongna , Li Yongyao , Li Xiaobin , Wu Jian , Qian Shizhi , Li Fengchen 
Published: February 2018

AbstractWe proposed an innovative method to achieve dynamic control of particle separation by employing viscoelastic fluids in deterministic lateral displacement (DLD) arrays. The effects of shear-thinning and elasticity of working fluids on the critical separation size in DLD arrays are investigated. It is observed that each effect can lead to the variation of the critical separation size by approximately 40%. Since the elasticity strength of the fluid is related to the shear rate, the dynamic control can for the first time be easily realized through tuning the flow rate in microchannels.
Selective state spectroscopy and multifractality in disordered Bose-Einstein condensates: a numerical study
Authors: Werner Miklós Antal , Demler Eugene , Aspect Alain , Zaránd Gergely 
Published: February 2018

AbstractWe propose to apply a modified version of the excitation scheme introduced by Volchkov et al. on bosons experiencing hyperfine state dependent disorder to address the critical state at the mobility edge of the Anderson localization transition, and to observe its intriguing multifractal structure. An optimally designed, spatially focused external radio frequency pulse can be applied to generate transitions to eigenstates in a narrow energy window close to the mobility edge, where critical scaling and multifractality emerge. Alternatively, two-photon laser scanning microscopy is proposed to address individual localized states even close to the transition. The projected image of the cloud is shown to inherit multifractality and to display universal density correlations. Interactions – unavoidably present – are taken into account by solving the Gross-Pitaevskii equations, and their destructive effect on the spectral resolution and the multifractal spectrum is analyzed. Time of flight images of the excited states are predicted to show interference fringes in the localized phase, while they allow one to map equal energy surfaces deep in the metallic phase.
Factors influencing intention to obtain the HPV vaccine in South East Asian and Western Pacific regions: A systematic review and meta-analysis
Authors: Santhanes Diviya , Yong Che Pui , Yap Yan Ye , Saw Pui San , Chaiyakunapruk Nathorn , Khan Tahir Mehmood 
Published: February 2018

AbstractSince licensing in 2006, there has been poor uptake of the HPV vaccine among the targeted population in the South East Asia Region (SEAR) and Western Pacific Region (WPR). A systematic review was conducted to identify the studies exploring the relationship between factors and intention for HPV vaccination among women in SEAR and WPR countries. Nineteen studies were identified as suitable for qualitative synthesis, and three as suitable for meta-analysis. Most women had a positive intention to have an HPV vaccine (range 57%–85%). Having a positive intention to vaccinate was significantly higher among women not aware of HPV infection (OR: 1.34, 95% CI: 1.02–1.76) and HPV vaccine (OR: 1.57, 95% CI: 1.26–1.96). Lower knowledge level and less confidence in safety and efficacy of the vaccine, negatively affected intention to vaccinate. Perceiving the vaccine to be expensive, low perception of contracting HPV infection and cervical cancer, and lack of concrete recommendations from healthcare providers also negatively affected intention to vaccinate. This review suggests the decision-making processes of women in SEAR and WPR is influenced by the cost of vaccination, perceived efficacy and safety of vaccine, provision of information on vaccination, and the awareness about HPV infection and the HPV vaccine.
Clinical Utility of FDG PET/CT in Patients with Autoimmune Pancreatitis: a Case-Control Study
Authors: Cheng Mei-Fang , Guo Yue Leon , Yen Ruoh-Fang , Chen Yi-Chieh , Ko Chi-Lun , Tien Yu-Wen , Liao Wei-Chih , Liu Chia-Ju , Wu Yen-Wen , Wang Hsiu-Po 
Published: February 2018

AbstractAutoimmune pancreatitis (AIP) shares overlapping clinical features with pancreatic cancer (PC). Importantly, treatment of the two conditions is different. We investigated the clinical usefulness of 18F-fluorodeoxyglucose (FDG) positron emission tomography/computed tomography (PET/CT) in patients with suspected AIP before treatment. From September 2008 to July 2016, 53 patients with suspected AIP at National Taiwan University Hospital had PET/CT prior to therapy to exclude malignancy and evaluate the extent of inflammation. Their scans were compared with those from 61 PC patients. PET imaging features were analyzed using logistic regression. Significant differences in pancreatic tumor uptake morphology, maximum standardized uptake value, high-order primary tumor texture feature (i.e. high-gray level zone emphasis value), and numbers and location of extrapancreatic foci were found between AIP and PC. Using the prediction model, the area under curve of receiver-operator curve was 0.95 (P < 0.0001) with sensitivity, specificity, positive predictive, and negative predictive values of 90.6%, 84.0%, 87.9%, and 87.5% respectively, in differentiating AIP from PC. FDG PET/CT offers high sensitivity, albeit slightly lower specificity in differentiating AIP from PC. Nonetheless, additional systemic inflammatory foci detected by the whole body PET/CT help confirm diagnosis of AIP in these patients before initiating steroid therapy, especially when biopsy is inconclusive.
Input-Independent Energy Harvesting in Bistable Lattices from Transition Waves
Authors: Hwang Myungwon , Arrieta Andres F. 
Published: February 2018

AbstractWe demonstrate the utilisation of transition waves for realising input-invariant, frequency-independent energy harvesting in 1D lattices of bistable elements. We propose a metamaterial-inspired design with an integrated electromechanical transduction mechanism to the unit cell, rendering the power conversion capability an intrinsic property of the lattice. Moreover, focusing of transmitted energy to desired locations is demonstrated numerically and experimentally by introducing engineered defects in the form of perturbation in mass or inter-element forcing. We achieve further localisation of energy and numerically observe a breather-like mode for the first time in this type of lattice, improving the harvesting performance by an order of magnitude. Our approach considers generic bistable unit cells and thus provides a universal mechanism to harvest energy and realise metamaterials effectively behaving as a capacitor and power delivery system.
Rapid oxygen diffusion during high temperature alteration of zircon
Authors: Roberts Nick M. W. , Yang Qiong-Yan , Santosh M. 
Published: February 2018

AbstractThe mineral zircon through its isotopic and elemental signatures comprises the greatest archive recording the evolution of Earth’s continental crust. Recognising primary from secondary zircon compositional signatures is thus important for the accurate interpretation of this archive. We report two examples of metasedimentary rocks from high-grade shear zones within the Southern Granulite Belt of India, where anomalously high and homogeneous oxygen isotope signatures indicate disturbance of this isotopic system. Utilising the combined U-Pb-Hf-O and trace element signatures from these zircon grains, we postulate that fluid-assisted alteration has led to complete resetting of the oxygen isotope signatures. This case study presents a rarely observed natural example of potentially fast diffusion of oxygen under hydrous conditions. Given the pervasive nature of fluid interaction within high-grade and highly deformed rocks, we expect that such isotopic disturbance might be more common to nature than is currently reported. A lack of correlation between isotopic disturbance with cathodoluminescence or Th/U values, suggests that these altered zircon grains would not clearly be classified as metamorphic, in which case they would be expected to yield primary compositions. Caution is therefore advised when using detrital δ18O zircon compilations without a high level of scrutiny for primary versus secondary compositions.
Virtual Touch IQ elastography reduces unnecessary breast biopsies by applying quantitative “rule-in” and “rule-out” threshold values
Authors: Kapetas Panagiotis , Clauser Paola , Woitek Ramona , Pinker Katja , Bernathova Maria , Helbich Thomas H. , Baltzer Pascal A. 
Published: February 2018

AbstractOur purpose was to evaluate Virtual Touch IQ (VTIQ) elastography and identify quantitative “rule-in” and “rule-out” thresholds for the probability of malignancy, which can help avoid unnecessary breast biopsies. 189 patients with 196 sonographically evident lesions were included in this retrospective, IRB-approved study. Quantitative VTIQ images of each lesion measuring the respective maximum Shear Wave Velocity (SWV) were obtained. Paired and unpaired, non-parametric statistics were applied for comparisons as appropriate. ROC-curve analysis was used to analyse the diagnostic performance of VTIQ and to specify “rule-in” and “rule-out” thresholds for the probability of malignancy. The standard of reference was either histopathology or follow-up stability for >24 months. 84 lesions were malignant and 112 benign. Median SWV of benign lesions was significantly lower than that of malignant lesions (p < 0.001). The application of a “rule-out” threshold of 1.9 m/s lead to a sensitivity of >98% with a concomitant significant (p = 0.032) reduction in false positive cases of almost 15%, whereas a “rule-in” threshold of 6.5 m/s suggested a probability of malignancy of >95%. In conclusion, VTIQ elastography accurately differentiates malignant from benign breast lesions. The application of quantitative “rule-in” and “rule-out” thresholds is feasible and allows reduction of unnecessary benign breast biopsies by almost 15%.
Localization of lipopolysaccharide from Escherichia Coli into human atherosclerotic plaque
Authors: Carnevale Roberto , Nocella Cristina , Petrozza Vincenzo , Cammisotto Vittoria , Pacini Luca , Sorrentino Veronica , Martinelli Ombretta , Irace Luigi , Sciarretta Sebastiano , Frati Giacomo , Pastori Daniele , Violi Francesco 
Published: February 2018

AbstractExperimental studies showed that gut-derived lipopolysaccharide (LPS) is pro-atherogenic, however, its relationship with human atherosclerosis is still to be defined. We investigate if gut-derived LPS from Escherichia Coli localizes in human carotid plaque and its potential role as pro-inflammatory molecule in the atherosclerotic lesion. LPS from Escherichia Coli and Toll-like receptor 4 (TLR4) were studied in specimens from carotid and thyroid arteries of 10 patients undergoing endarterectomy and 15 controls matched for demographic and clinical characteristics. Blood LPS were significantly higher in patients compared to controls. Immunochemistry analysis revealed positivity for antibodies against LPS and TLR4 coincidentally with positivity for CD68 only in the atherosclerotic plaque of carotid arteries but not in thyroid arteries; the positivity for LPS and TLR4 was greater in the area with activated macrophages. LPS concentration similar to that detected in atherosclerotic plaque resulted in a dose-dependent TLR4-mediated Nox2 up-regulation by human monocytes. These data provide the first evidence that LPS from Escherichia Coli localizes in human plaque and may contribute to atherosclerotic damage via TLR4-mediated oxidative stress.
Quantitative Assessment of Liver Steatosis and Affected Pathways with Molecular Imaging and Proteomic Profiling
Authors: Urasaki Yasuyo , Zhang Chi , Cheng Ji-Xin , Le Thuc T. 
Published: February 2018

AbstractCurrent assessment of non-alcoholic fatty liver disease (NAFLD) with histology is time-consuming, insensitive to early-stage detection, qualitative, and lacks information on etiology. This study explored alternative methods for fast and quantitative assessment of NAFLD with hyperspectral stimulated Raman scattering (SRS) microscopy and nanofluidic proteomics. Hyperspectral SRS microscopy quantitatively measured liver composition of protein, DNA, and lipid without labeling and sensitively detected early-stage steatosis in a few minutes. On the other hand, nanofluidic proteomics quantitatively measured perturbations to the post-translational modification (PTM) profiles of selective liver proteins to identify affected cellular signaling and metabolic pathways in a few hours. Perturbations to the PTM profiles of Akt, 4EBP1, BID, HMGCS2, FABP1, and FABP5 indicated abnormalities in multiple cellular processes including cell cycle regulation, PI3K/Akt/mTOR signaling cascade, autophagy, ketogenesis, and fatty acid transport. The integrative deployment of hyperspectral SRS microscopy and nanofluidic proteomics provided fast, sensitive, and quantitative assessment of liver steatosis and affected pathways that overcame the limitations of histology.
A dataset mapping the potential biophysical effects of vegetation cover change
Authors: Duveiller Gregory , Hooker Josh , Cescatti Alessandro 
Published: February 2018

AbstractChanging the vegetation cover of the Earth has impacts on the biophysical properties of the surface and ultimately on the local climate. Depending on the specific type of vegetation change and on the background climate, the resulting competing biophysical processes can have a net warming or cooling effect, which can further vary both spatially and seasonally. Due to uncertain climate impacts and the lack of robust observations, biophysical effects are not yet considered in land-based climate policies. Here we present a dataset based on satellite remote sensing observations that provides the potential changes i) of the full surface energy balance, ii) at global scale, and iii) for multiple vegetation transitions, as would now be required for the comprehensive evaluation of land based mitigation plans. We anticipate that this dataset will provide valuable information to benchmark Earth system models, to assess future scenarios of land cover change and to develop the monitoring, reporting and verification guidelines required for the implementation of mitigation plans that account for biophysical land processes.
Inter-comparison of multiple statistically downscaled climate datasets for the Pacific Northwest, USA
Authors: Jiang Yueyang , Kim John B. , Still Christopher J. , Kerns Becky K. , Kline Jeffrey D. , Cunningham Patrick G. 
Published: February 2018

AbstractStatistically downscaled climate data have been widely used to explore possible impacts of climate change in various fields of study. Although many studies have focused on characterizing differences in the downscaling methods, few studies have evaluated actual downscaled datasets being distributed publicly. Spatially focusing on the Pacific Northwest, we compare five statistically downscaled climate datasets distributed publicly in the US: ClimateNA, NASA NEX-DCP30, MACAv2-METDATA, MACAv2-LIVNEH and WorldClim. We compare the downscaled projections of climate change, and the associated observational data used as training data for downscaling. We map and quantify the variability among the datasets and characterize the spatio-temporal patterns of agreement and disagreement among the datasets. Pair-wise comparisons of datasets identify the coast and high-elevation areas as areas of disagreement for temperature. For precipitation, high-elevation areas, rainshadows and the dry, eastern portion of the study area have high dissimilarity among the datasets. By spatially aggregating the variability measures into watersheds, we develop guidance for selecting datasets within the Pacific Northwest climate change impact studies.
Arthropods dataset from different genetically modified maize events and associated controls
Authors: Pálinkás Zoltán , Zalai Mihály , Szénási Ágnes , Dorner Zita , Kiss József , North Samuel , Woodward Guy , Balog Adalbert 
Published: February 2018

AbstractArthropods from four genetically modified (GM) maize hybrids (coleopteran resistant, coleopteran and lepidopteran resistant, lepidopteran resistant+herbicide tolerant and coleopteran resistant and herbicide tolerant) and non-GM varieties were sampled during a two-year field assessment. A total number of 363 555 arthropod individuals were collected. This represents the most comprehensive arthropod dataset from GM maize, and together with weed data, is reasonable to determine functional groups of arthropods and interactions between species. Trophic groups identified from both phytophagous and predatory arthropods were previously considered non-target organisms on which possible detrimental effects of Bacillus thuringiensis (Bt) toxins may have been directly (phytophagous species) or indirectly (predators) detected. The high number of individuals and species and their dynamics through the maize growing season can predict that interactions are highly correlational, and can thus be considered a useful tool to assess potential deleterious effects of Bt toxins on non-target organisms, serving to develop biosafety risk hypotheses for invertebrates exposed to GM maize plants.
Algorithm for islanding detection in photovoltaic generator network connected to low‐voltage grid
Authors: Gupta Nikita , Garg Rachana 
Published: February 2018

A new islanding detection method has been developed based on the analysis of negative sequence components of the voltage at the point of common coupling, using wavelet packet transform. The binary tree classifier is used for decision‐making mechanism. The proposed algorithm is able to reduce the non‐detection zone to zero and is able to detect islanding within 5 ms. The developed algorithm can also discriminate between the islanding and other events in the system. The simulation results carried out by MATLAB along with Simulink toolbox are used to test the performance of the proposed algorithm in a photovoltaic generator network connected to the low‐voltage grid.
The cosmological analysis of X-ray cluster surveys
Authors: Valotti A. , Pierre M. , Farahi A. , Evrard A. , Faccioli L. , Sauvageot J.-L. , Clerc N. , Pacaud F. 
Published: February 2018

Context. This paper is the fourth of a series evaluating the ASpiX cosmological method, based on X-ray diagrams, which are constructed from simple cluster observable quantities, namely: count rate (CR), hardness ratio (HR), core radius (rc), and redshift. Aims. Following extensive tests on analytical toy catalogues (Paper III), we present the results of a more realistic study over a 711 deg2 template-based maps derived from a cosmological simulation.
Methods. Dark matter haloes from the Aardvark simulation have been ascribed luminosities, temperatures, and core radii, using local scaling relations and assuming self-similar evolution. The predicted X-ray sky-maps were converted into XMM event lists, using a detailed instrumental simulator. The XXL pipeline runs on the resulting sky images, produces an observed cluster catalogue over which the tests have been performed. This allowed us to investigate the relative power of various combinations of the CR, HR, rc, and redshift information. Two fitting methods were used: a traditional Markov chain Monte Carlo (MCMC) approach and a simple minimisation procedure (Amoeba) whose mean uncertainties are a posteriori evaluated by means of synthetic catalogues. The results were analysed and compared to the predictions from the Fisher analysis (FA).
Results. For this particular catalogue realisation, assuming that the scaling relations are perfectly known, the CR-HR combination gives σ8 and Ωm at the 10% level, while CR-HR-rc-z improves this to ≤3%. Adding a second HR improves the results from the CR-HR1-rc combination, but to a lesser extent than when adding the redshift information. When all coefficients of the mass-temperature relation (M-T, including scatter) are also fitted, the cosmological parameters are constrained to within 5–10% and larger for the M-T coefficients (up to a factor of two for the scatter). The errors returned by the MCMC, those by Amoeba and the FA predictions are in most cases in excellent agreement and always within a factor of two. We also study the impact of the scatter of the mass-size relation (M-Rc) on the number of detected clusters: for the cluster typical sizes usually assumed, the larger the scatter, the lower the number of detected objects.
Conclusions. The present study confirms and extends the trends outlined in our previous analyses, namely the power of X-ray observable diagrams to successfully and easily fit at the same time, the cosmological parameters, cluster physics, and the survey selection, by involving all detected clusters. The accuracy levels quoted should not be considered as definitive. A number of simplifying hypotheses were made for the testing purpose, but this should affect any method in the same way. The next publication will consider in greater detail the impact of cluster shapes (selection and measurements) and of cluster physics on the final error budget by means of hydrodynamical simulations.