bluuebunny/crossref_metadata_2025_split huggingface dataset:
The VAR2CSA malaria protein efficiently retrieves circulating tumor cells in an EpCAM-independent manner
Authors: Agerbæk Mette Ø. , Bang-Christensen Sara R. , Yang Ming-Hsin , Clausen Thomas M. , Pereira Marina A. , Sharma Shreya , Ditlev Sisse B. , Nielsen Morten A. , Choudhary Swati , Gustavsson Tobias , Sorensen Poul H. , Meyer Tim , Propper David , Shamash Jonathan , Theander Thor G. , Aicher Alexandra , Daugaard Mads , Heeschen Christopher , Salanti Ali 
Published: August 2018

AbstractIsolation of metastatic circulating tumor cells (CTCs) from cancer patients is of high value for disease monitoring and molecular characterization. Despite the development of many new CTC isolation platforms in the last decade, their isolation and detection has remained a challenge due to the lack of specific and sensitive markers. In this feasibility study, we present a method for CTC isolation based on the specific binding of the malaria rVAR2 protein to oncofetal chondroitin sulfate (ofCS). We show that rVAR2 efficiently captures CTCs from hepatic, lung, pancreatic, and prostate carcinoma patients with minimal contamination of peripheral blood mononuclear cells. Expression of ofCS is present on epithelial and mesenchymal cancer cells and is equally preserved during epithelial–mesenchymal transition of cancer cells. In 25 stage I–IV prostate cancer patient samples, CTC enumeration significantly correlates with disease stage. Lastly, rVAR2 targets a larger and more diverse population of CTCs compared to anti-EpCAM strategies.
Enhanced photon collection enables four dimensional fluorescence nanoscopy of living systems
Authors: Masullo Luciano A. , Bodén Andreas , Pennacchietti Francesca , Coceano Giovanna , Ratz Michael , Testa Ilaria 
Published: August 2018

AbstractThe theoretically unlimited spatial resolution of fluorescence nanoscopy often comes at the expense of time, contrast and increased dose of energy for recording. Here, we developed MoNaLISA, for Molecular Nanoscale Live Imaging with Sectioning Ability, a nanoscope capable of imaging structures at a scale of 45–65 nm within the entire cell volume at low light intensities (W-kW cm−2). Our approach, based on reversibly switchable fluorescent proteins, features three distinctly modulated illumination patterns crafted and combined to gain fluorescence ON–OFF switching cycles and image contrast. By maximizing the detected photon flux, MoNaLISA enables prolonged (40–50 frames) and large (50 × 50 µm2) recordings at 0.3–1.3 Hz with enhanced optical sectioning ability. We demonstrate the general use of our approach by 4D imaging of organelles and fine structures in epithelial human cells, colonies of mouse embryonic stem cells, brain cells, and organotypic tissues.
Ideal Cardiovascular Health Metrics Associated with Reductions in the Risk of Extracranial Carotid Artery Stenosis: a Population-based Cohort Study
Authors: Wang Junyou , Shao Bo , Da Lin  , He Xijun , Zhang Yongqiang , Zhang Li , Jiang Tian , Xu Jinzhong , Wang Youxin , Wu Jing , Zhou Yong , Chen Junzheng , Teng Lingfang 
Published: August 2018

AbstractThe cardiovascular health (CVH) metrics are closely related to the risk of stroke. Extracranial carotid artery stenosis (ECAS) represents an important risk factor for ischemic stroke. The present study aims to explore the longitudinal effect of the baseline CVH metrics on the development of ECAS. Totally 5,440 participants were randomly enrolled in the Asymptomatic Polyvascular Abnormalities Community study from 2010 to 2011. Information regarding the seven CVH metrics was collected at baseline. ECAS was assessed by performing carotid duplex sonography at baseline (2010–2011) and during the follow-up (2012–2013). Finally 3,487 subjects were included, and 976 participants developed ECAS during the 2-year follow-up. The optimum CVH status was associated with a 42% (95% confidence interval: 0.40–0.85) decreased risk of the incidence of ECAS after adjusting for age, sex, weight, education, income, alcohol use, waist-hip ratio, triglycerides, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, uric acid, homocysteine, and C-reactive protein. Ideal physical activity, total cholesterol and fasting blood glucose were independent protective factors of ECAS. In this cohort study, the ideal baseline CVH status was negatively associated with the occurrence of ECAS during the follow-up. This study provides practical insight for further developing effective screening strategies or implementing the best medical treatment.
Impact of the Reticular Stress and Unfolded Protein Response on the inflammatory response in endometrial stromal cells
Authors: Grasso E. , Gori S. , Soczewski E. , Fernández L. , Gallino L. , Vota D. , Martínez G. , Irigoyen M. , Ruhlmann C. , Lobo T. F. , Salamone G. , Mattar R. , Daher S. , Leirós C. Pérez , Ramhorst R. 
Published: August 2018

AbstractDuring decidualization, endometrial stromal cells undergo reticular stress (RS) and unfolded protein response (UPR), allowing the endoplasmic reticulum-expansion and immunomodulators production. Physiological RS generates the activation of sensing proteins, inflammasome activation and mature-IL-1β secretion, associated with pro-implantatory effects. We focus on the impact of RS and UPR on decidualized cells and whether they induce a physiological sterile inflammatory response through IL-1β production. Human endometrial stromal cell line (HESC) after decidualization treatment with MPA + dibutyryl-cAMP (Dec) increased the expression of RS-sensors (ATF6, PERK and IRE1α) and UPR markers (sXBP1 and CHOP) in comparison with Non-dec cells. Then we found increased NLRP3 expression in Dec cells compared with Non-dec cells. In fact STF-083010 (an IRE1α inhibitor) prevented this increase. Downstream, increased levels of active caspase-1 on Dec cells were detected by FAM-Flica Caspase-1 associated with an increase in IL-1β production. Moreover, the treatment with STF-083010 decreased the invasion index observed in Dec cells, evaluated by an in vitro model of implantation. In endometrial biopsies from recurrent spontaneous abortion patients an increased expression of IRE1α was found in comparison with fertile women; while recurrent implantation failure samples showed a lower expression of sXBP1, TXNIP and NLRP3 than fertile women, suggesting that RS/UPR tenors might condition endometrial receptivity.
Macroecological patterns of the phytoplankton production of polyunsaturated aldehydes
Authors: Cózar Andrés , Morillo-García Soledad , Ortega María J. , Li Qian P. , Bartual Ana 
Published: August 2018

AbstractThe polyunsaturated aldehydes (PUAs) are bioactive metabolites commonly released by phytoplankton species. Based primarily on laboratory experiments, PUAs have been implicated in deleterious effects on herbivores and competing phytoplankton species or in the regulation of the rates of bacterial organic matter remineralization; however, the role of the PUAs at an ecosystem level is still under discussion. Using data of PUA production in natural phytoplankton assemblages over a wide range of conditions, we analyzed macroecological patterns aiming for a comprehensive environmental contextualization that will further our understanding of the control and ecologic role played by these compounds. PUA composition changed from the predominance of decadienal in oligotrophy, octadienal in eutrophy, and heptadienal at intermediate conditions. The production of PUAs per unit biomass also showed a strong relationship with the trophic status, sharply increasing towards oligotrophic conditions and with small-sized cells reaching the highest production rates. High ratios of dissolved inorganic nitrogen to dissolved inorganic phosphorus also promoted PUA production, albeit to a considerably lesser extent. Although the allelopathic use of PUAs to outcompete other phytoplankton or reduce herbivory may be key in some environments and interactions, the macroecological patterns found here, showing higher production towards the poorest waters and among the small species typically populating these environments, support and link at the large scale the hypotheses of the nutrient-derived stress as driver for the production of PUAs together with the use of these compounds as boosters for the nutrient remineralization.
Pregnancy Outcomes in Women of Advanced Maternal Age: a Retrospective Cohort Study from China
Authors: Shan Dan , Qiu Pei-Yuan , Wu Yu-Xia , Chen Qian , Li Ai-Lin , Ramadoss Sivakumar , Wang Ran-Ran , Hu Ya-Yi 
Published: August 2018

AbstractThis retrospective cohort study attempts to investigate pregnancy complications and adverse pregnancy outcomes in women of advanced maternal age (AMA). Data were extracted from electronic medical records system at West China Second University Hospital of Sichuan University from January 2013 to July 2016. The study cohort consisted 8 subgroups of women on 4 different age levels (20–29 years, 30–34 years, 35–39 years and ≥40 years) and 2 different parities (primiparity and multiparity). In the study period, 38811 women gave birth at our hospital, a randomized block was used to include 2800 women of singleton pregnancy >28 gestational weeks, with 350 patients in each subgroup. Maternal complications and fetal outcomes were collected and defined according to relevant guidelines. Confounding factors representing maternal demographic characteristics were identified from previous studies and analysed in multivariate analysis. There was an increasing trend for the risks of adverse pregnancy outcomes with increasing age, especially in AMA groups. Our study showed that AMA, primiparity, maternal overweight or obesity, lower educational level and residence in rural area increased pregnancy complications and adverse fetal outcomes. Increased professional care as well as public concern is warranted.
Regulation of cofilin phosphorylation in glomerular podocytes by testis specific kinase 1 (TESK1)
Authors: Wang Liming , Buckley Anne F. , Spurney Robert F. 
Published: August 2018

AbstractExpression of a constitutively active Rho A (V14Rho) in podocytes in vivo induces albuminuria and foot process (FP) effacement. These effects may be mediated by the Rho A effector Rho kinase (ROK); but inhibition of ROK with Y27632 failed to attenuate albuminuria or FP effacement in V14Rho mice. ROK activates LIM kinases (LIMKs), which phosphorylate and inhibit the actin depolymerizing factor cofilin 1 (CFL1). Sustained phosphorylation of CFL1 is implicated in human nephrotic diseases, but Y27632 did not inhibit phosphorylation of CFL1 in vivo, despite effective ROK inhibition. CFL1 is also phosphorylated by testis-specific kinase 1 (TESK1) on the same serine residue. TESK1 was expressed in podocytes, and, similar to the in vivo situation, Y27632 had little effect on phospho-CFL1 (pCFL1) levels in cultured podocytes. In contrast, Y27632 reduced pCFL1 levels in TESK1 knockout (KO) cells. ROK inhibition enhanced podocyte motility but, the motility promoting effect of Y27632 was absent in TESK1 KO podocytes. Thus, TESK1 regulates podocyte cytoskeletal dynamics in glomerular podocytes and may play an important role in regulating glomerular filtration barrier integrity in glomerular disease processes.
Gold nanoparticles-induced cytotoxicity in triple negative breast cancer involves different epigenetic alterations depending upon the surface charge
Authors: Surapaneni Sunil Kumar , Bashir Shafiya , Tikoo Kulbhushan 
Published: August 2018

AbstractGold nanoparticles (AuNPs) are used enormously in different cancers but very little is known regarding their molecular mechanism and surface charge role in the process of cell death. Here, we elucidate the molecular mechanism by which differentially charged AuNPs induce cytotoxicity in triple negative breast cancer (TNBC) cells. Cytotoxicity assay revealed that both negatively charged (citrate-capped) and positively charged (cysteamine-capped) AuNPs induced cell-death in a dose-dependent manner. We provide first evidence that AuNPs-induced oxidative stress alters Wnt signalling pathway in MDA-MB-231 and MDA-MB-468 cells. Although both differentially charged AuNPs induced cell death, the rate and mechanism involved in the process of cell death were different. Negatively charged AuNPs increased the expression of MKP-1, dephosphorylated and deacetylated histone H3 at Ser10 and K9/K14 residues respectively whereas, positively charged AuNPs decreased the expression of MKP-1, phosphorylated and acetylated histone H3 at Ser 10 and K9/K14 residues respectively. High-resolution transmission electron microscopy (HRTEM) studies revealed that AuNPs were localised in cytoplasm and mitochondria of MDA-MB-231 cells. Interestingly, AuNPs treatment makes MDA-MB-231 cells sensitive to 5-fluorouracil (5-FU) by decreasing the expression of thymidylate synthetase enzyme. This study highlights the role of surface charge (independent of size) in the mechanisms of toxicity and cell death.
Parasite transmission between trophic levels stabilizes predator–prey interaction
Authors: Rogawa Akiyoshi , Ogata Shigeki , Mougi Akihiko 
Published: August 2018

AbstractManipulative parasites that promote their transmission by altering their host’s phenotype are widespread in nature, which suggests that host manipulation allows the permanent coexistence of the host with the parasite. However, the underlying mechanism by which host manipulation affects community stability remains unelucidated. Here, using a mathematical model, we show that host manipulation can stabilise community dynamics. We consider systems wherein parasites are transmitted between different trophic levels: intermediate host prey and final host predator. Without host manipulation, the non-manipulative parasite can destabilise an otherwise globally stable prey–predator system, causing population cycles. However, host manipulation can dampen such population cycles, particularly when the manipulation is strong. This finding suggests that host manipulation is a consequence of self-organized behavior of the parasite populations that allows permanent coexistence with the hosts and plays a key role in community stability.
Hazardous gases (CO, NOx, CH4 and C3H8) released from CO2 fertilizer unit lead to oxidative damage and degrades photosynthesis in strawberry plants
Authors: Muneer Sowbiya , Lee Jeong Hyun 
Published: August 2018

AbstractCO2 boilers/direct heating systems used in greenhouses often lead to incomplete combustion, which results in the formation of hazardous gases, such as carbon monoxide (CO), nitroxide (NOX) and other hydrocarbons. In this study, strawberry plants that were grown on rockwool cubes were transferred to airtight bottles and treated with CO, NOX, CH4 and C3H8 gases for 1–48 hours. Oxidative damage due to hazardous gases was observed, as indicated by H2O2 and $${{\bf{O}}}_{{\bf{2}}}^{{\boldsymbol{-}}1}$$

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2

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determination. Photosynthetic pigments were reduced, and stomatal guard cells were damaged and remained closed compared to the control. The activity of other photosynthetic parameters was negatively related to hazardous gases. Reduction in the expression of multiprotein complexes was highly observed under hazardous gas treatments. This study highlighted that hazardous gases (CO, NOX, CH4 and C3H8) emitted due to incomplete combustion of CO2 fertilization units/or direct heating systems resulted in the formation of ROS in shoots and limited photosynthetic metabolism. We predicted that major steps must be incorporated to improve the efficiency of CO2 boiler/heating systems to decrease the emission of these hazardous gases and other hydrocarbons and to reduce the observed risks that are key to the reduction of crops.
Subsynchronous resonance assessment for a large system with multiple series compensated transmission circuits
Authors: Cheng Yunzhi , Huang Shun Hsien (Fred) , Rose Jonathan , Pappu Venkata Ajay , Conto Jose 
Published: August 2018

Generators connecting to a series compensated transmission system can be exposed to subsynchronous resonance (SSR) and require a comprehensive vulnerability assessment. This study presents an SSR assessment framework and tools to more efficiently assess all types of generation in a large system with multiple generators and meshed series capacitors. The developed framework covers torsional interaction, torque amplification, and induction generator effect/control interaction and includes three assessment stages: topology screening, frequency scan, and detailed electromagnetic transient study. Topology screening based on max‐flow min‐cut theorem is applied to identify the set of transmission outages forming radial connections between generators and series capacitors. Frequency scan analysis is then conducted to further analyse the SSR vulnerability with all the possible combinations of transmission outages identified in the topology screening results. Nearby generators are modelled by frequency‐dependent impedance tables, which consider the equivalent impedance looking into the generators over the range of 1–120 Hz. An electromagnetic transient simulation will be required to confirm the SSR vulnerability identified in the frequency scan analysis. The developed framework and tools have successfully been applied to a large power system with multiple series compensated circuits to ensure reliable generation interconnection and operation.
The XXL Survey
Authors: Logan C. H. A. , Maughan B. J. , Bremer M. N. , Giles P. , Birkinshaw M. , Chiappetti L. , Clerc N. , Faccioli L. , Koulouridis E. , Pacaud F. , Pierre M. , Ramos-Ceja M. E. , Vignali C. , Willis J. 
Published: August 2018

Context. The XMM-XXL survey has used observations from the XMM-Newton observatory to detect clusters of galaxies over a wide range in mass and redshift. The moderate PSF (FWHM ~ 6″ on-axis) of XMM-Newton means that point sources within or projected onto a cluster may not be separated from the cluster emission, leading to enhanced luminosities and affecting the selection function of the cluster survey.
Aims. We present the results of short Chandra observations of 21 galaxy clusters and cluster candidates at redshifts z > 1 detected in the XMM-XXL survey in X-rays or selected in the optical and infra-red.
Methods. With the superior angular resolution of Chandra, we investigate whether there are any point sources within the cluster region that were not detected by the XMM-XXL analysis pipeline, and whether any point sources were misclassified as distant clusters.
Results. Of the 14 X-ray selected clusters, 9 are free from significant point source contamination, either having no previously unresolved sources detected by Chandra or with less than about 10% of the reported XXL cluster flux being resolved into point sources. Of the other five sources, one is significantly contaminated by previously unresolved AGN, and four appear to be AGN misclassified as clusters. All but one of these cases are in the subset of less secure X-ray selected cluster detections and the false positive rate is consistent with that expected from the XXL selection function modelling. We also considered a further seven optically selected cluster candidates associated with faint XXL sources that were not classed as clusters. Of these, three were shown to be AGN by Chandra, one is a cluster whose XXL survey flux was highly contaminated by unresolved AGN, while three appear to be uncontaminated clusters. By decontaminating and vetting these distant clusters, we provide a pure sample of clusters at redshift z > 1 for deeper follow-up observations, and demonstrate the utility of using Chandra snapshots to test for AGN in surveys with high sensitivity but poor angular resolution.
Memory Impairment Allied to Temporal Lobe Epilepsy and its Deterioration by Phenytoin: A Highlight on Ameliorative Effects of Levetiracetam in Mouse Model
Authors: Mohan Asher , Krishna Kamsagara 
Published: August 2018

Abstract
Background/Objectives Memory impairment (MI) and epilepsy go hand in hand, mainly in conditions of temporal lobe epilepsy (TLE). This disease comorbidity has been reported to worsen upon treatment. Hence this study aims to evaluate the extent of aggravating effect of phenytoin (PHT) at normal and reduced doses on MI associated with TLE and additionally assesses the protective effect of levetiracetam (LEV) on these adverse effects.
Methods Swiss albino mice of either sex (n = 36) were used for this study in which seizures were induced by intraperitoneal administration of pilocarpine (300 mg/kg i.p.) followed by evaluation of antiepileptic activity by technique of Racine's scale for convulsive scores. Errors (a factor denoting MI) were assessed using radial arm maze. Finally brain biochemical measures of acetylcholinesterase and glutamate along with cresyl violet staining and estimation of total neuronal number of the hippocampus were performed.
Results Exacerbation of MI by PHT was observed, where the extent of MI was found to be lesser in the reduced dose approach (PHTR: 28.50 ± 1.03; p ≤ 0.05). However, this tactic in dose reduction was interfered with the antiepileptic potential of the drug. Attenuation of MI upon combining with LEV, without an interference in the principal treatment, was observed equally in the behavioral and brain aspects of the study (PHTN + LEV: 1.33 ± 0.33 and PHTR + LEV: 1.00 ± 0.17; p ≤ 0.05).
Conclusions The promising effects of LEV could thus aid in proposing a new management remedy for TLE to minimize the adverse effect associated with it.
Arterial Endovascular Interventions in Acute Mesenteric Ischemia
Authors: Miller Geoffrey , Stone James , Wilkins Luke 
Published: August 2018

AbstractAcute mesenteric ischemia (AMI) is a true medical emergency and requires a multi-disciplinary treatment approach. AMI occurs when there is a sudden decrease in blood flow resulting in hypoperfusion to the intestines and may lead to bowel infarction. There are many potential etiologies of AMI that include arterial embolus, arterial, or venous thrombosis; traumatic injury; aortic dissection; intestinal obstruction; non-occlusive mesenteric ischemia, and vasculitis. Given mortality rates that approach 90%, rapid diagnosis is essential to decrease risk of bowel infarction. While surgical management has traditionally been the treatment of choice, endovascular management is being used with increasing frequency. Further, utilizing endovascular treatment options together with surgical intervention has shown promising results. The endovascular approach to treatment of AMI will depend on the underlying etiology. In addition, the acuity of patient presentation, presence, or absence of bowel infarction; patient stability; and availability of a hybrid operating room will influence the treatment plan of a patient with AMI. Early diagnosis with advanced cross-sectional imaging along with assessment of the underlying risk factors will optimize chances of early intervention and improve patient outcomes.
Klassifikation subaxialer Halswirbelsäulenverletzungen
Authors: Schopper Clemens , Schultheiss Markus 
Published: August 2018

ZusammenfassungIm klinischen Alltag wurden Verletzungen der unteren Halswirbelsäule lange Zeit analog zu den Brust- und Lendenwirbelsäulenverletzungen gemäß der AO-Klassifikation beschrieben. Im Rahmen dieser Übersicht wird nun die neue AOSpine-Klassifikation für Verletzungen der subaxialen Halswirbelsäule vorgestellt.