bluuebunny/crossref_metadata_2025_split huggingface dataset:
Acceptability and feasibility of a school-based contraceptive clinic in a low-income community in South Africa
Authors: Khoza Nondumiso , Zulu Phindile , Shung-King Maylene 
Published: March 2019

AbstractAimTo investigate how acceptable and feasible a school-based contraceptive clinic (SBCC) would be in a low-income South African community.BackgroundTeenage pregnancy is an important issue in South Africa, with significant health and social consequences. Issues regarding lack of confidentiality in an intimate community, unwelcoming health workers, long distances to clinics and perceptions of contraceptive side effects may all inhibit contraceptive use by adolescents. Although SBCC has been initiated and investigated in other countries, this approach is inadequately researched in South Africa.MethodsA mixed method study was conducted to assess the attitudes of one community towards establishment of an SBCC in their area. Methods of data collection included: focus group discussions (FGDs) with teenage girls from a local high school; a key informant interview with the school principal; a structured survey, including open-ended questionnaires with randomly selected parents of teenage girls from the same community; and a documentary analysis to explore relevant legal and policy considerations.FindingsTeenage girls, the school principal and parents with teenage daughters largely supported the idea of an SBCC, but with concerns about confidentiality, the possibility of increased promiscuity and contraceptive side effects. While legal statutes and policies in South Africa do not pose any barriers to the establishment of an SBCC, some logistical barriers remain.
A Migration Model for the Polar Spiral Troughs of Mars
Authors: Bramson A. M. , Byrne S. , Bapst J. , Smith I. B. , McClintock T. 
Published: March 2019

AbstractMars' iconic polar spiral troughs are 400–1,000‐m‐deep depressions in the north polar layered deposits. As the north polar layered deposits accumulate, troughs migrate approximately poleward, anti‐parallel to the local wind patterns. Insolation is suspected to drive ice retreat through sublimation. Sublimation at the trough wall produces a growing sublimation lag that modulates further retreat; however, winds move material off the retreating slope faces, thinning the lag. Discontinuities in stratigraphy seen by radar highlight Trough Migration Paths (TMPs), which provide a record of the troughs' position, formation, and evolution to the present day. We investigate two adjacent troughs presently near 87°N to evaluate the mass balance conditions at those sites. We constrain the contribution of insolation‐induced sublimation to the migration in the observed TMPs. We present a phenomenological model that combines our simulations of the sublimation conditions at paleo‐trough surfaces with accumulation rates to create synthetic TMPs that are tunable to the observations. Models using nominal values of lag diffusivity, albedo, and atmospheric water vapor abundance and in which the trough walls have been covered in a lag on the order of millimeters thick and formed ~2.3 Myr ago match the observed trough migration and align with expectations of trough ages. Thicker lags, and/or older troughs, would generate TMPs of constant slope, which does not match the observed paths. We demonstrate the viability of our new theoretical model for predicting conditions that lead to trough migration, allowing us to connect observable TMPs to Martian climate processes.
Widespread losses of pollinating insects in Britain
Authors: Powney Gary D. , Carvell Claire , Edwards Mike , Morris Roger K. A. , Roy Helen E. , Woodcock Ben A. , Isaac Nick J. B. 
Published: March 2019

AbstractPollination is a critical ecosystem service underpinning the productivity of agricultural systems across the world. Wild insect populations provide a substantial contribution to the productivity of many crops and seed set of wild flowers. However, large-scale evidence on species-specific trends among wild pollinators are lacking. Here we show substantial inter-specific variation in pollinator trends, based on occupancy models for 353 wild bee and hoverfly species in Great Britain between 1980 and 2013. Furthermore, we estimate a net loss of over 2.7 million occupied 1 km2 grid cells across all species. Declines in pollinator evenness suggest that losses were concentrated in rare species. In addition, losses linked to specific habitats were identified, with a 55% decline among species associated with uplands. This contrasts with dominant crop pollinators, which increased by 12%, potentially in response agri-environment measures. The general declines highlight a fundamental deterioration in both wider biodiversity and non-crop pollination services.
Rapid mantle flow with power-law creep explains deformation after the 2011 Tohoku mega-quake
Authors: Agata Ryoichiro , Barbot Sylvain D. , Fujita Kohei , Hyodo Mamoru , Iinuma Takeshi , Nakata Ryoko , Ichimura Tsuyoshi , Hori Takane 
Published: March 2019

AbstractThe deformation transient following large subduction zone earthquakes is thought to originate from the interaction of viscoelastic flow in the asthenospheric mantle and slip on the megathrust that are both accelerated by the sudden coseismic stress change. Here, we show that combining insight from laboratory solid-state creep and friction experiments can successfully explain the spatial distribution of surface deformation in the first few years after the 2011 Mw 9.0 Tohoku-Oki earthquake. The transient reduction of effective viscosity resulting from dislocation creep in the asthenosphere explains the peculiar retrograde displacement revealed by seafloor geodesy, while the slip acceleration on the megathrust accounts for surface displacements on land and offshore outside the rupture area. Our results suggest that a rapid mantle flow takes place in the asthenosphere with temporarily decreased viscosity in response to large coseismic stress, presumably due to the activation of power-law creep during the post-earthquake period.
IL-4 together with IL-1β induces antitumor Th9 cell differentiation in the absence of TGF-β signaling
Authors: Xue Gang , Jin Guangxu , Fang Jing , Lu Yong 
Published: March 2019

AbstractIL-9-producing CD4+(Th9) cells are a subset of CD4+T-helper cells that are endowed with powerful antitumor capacity. Both IL-4 and TGF-β have been reported to be indispensable for Th9 cell-priming and differentiation. Here we show, by contrast, that Th9 cell development can occur in the absence of TGF-β signaling. When TGF-β was replaced by IL-1β, the combination of IL-1β and IL-4 efficiently promoted IL-9-producing T cells (Th9IL-4+IL-1β). Th9IL-4+ IL-1βcells are phenotypically distinct T cells compared to classic Th9 cells (Th9IL-4+TGF-β) and other Th cells, and are enriched for IL-1 and NF-κB gene signatures. Inhibition of NF-κB but not TGF-β-signaling negates IL-9 production by Th9IL-4+IL-1βcells. Furthermore, when compared with classic Th9IL-4+TGF-βcells, Th9IL-4+IL-1βcells are less exhausted, exhibit cytotoxic T effector gene signature and tumor killing function, and exert a superior antitumor response in a mouse melanoma model. Our study thus describes an alternative pathway for Th9 cell differentiation and provides a potential avenue for antitumor therapies.
Probing boundary conditions of Productive Failure and analyzing the role of young students’ collaboration
Authors: Mazziotti Claudia , Rummel Nikol , Deiglmayr Anne , Loibl Katharina 
Published: March 2019

AbstractProductive Failure (PF) facilitates students’ conceptual knowledge by delaying instruction until after problem solving. While PF is well investigated in middle and high school students, little is known about its effectiveness in younger students. Studies in younger samples, which implemented delayed instruction designs similar to those used in PF studies, showed mixed results. However, these studies did not implement two core design components of PF: (1) contrasting and comparing student-generated solutions and the canonical solution during the instructional phase (contrasting activity), and (2) students’ collaboration in small groups during the initial problem-solving phase. Both components can be expected to contribute to the effectiveness of PF. In a quasi-experimental study with 228 fifth graders, we implemented the first component (contrasting activity) with all students to establish whether under this condition, problem solving prior to instruction would be more effective for younger students’ conceptual knowledge acquisition than direct instruction (i.e., problem solving after instruction). Further, we experimentally tested the effect of the second component (collaborative vs. individual problem solving) on students’ conceptual knowledge and the number of solution ideas generated during initial problem solving. We found no empirical support for either of our hypotheses. To explore the extent to which students’ collaboration actually achieved its potential and relates to students’ conceptual knowledge and solution ideas in PF, we conducted analyses of collaborative processes. Our study adds to the mixed results regarding the superiority of problem solving prior to instruction for young students, thus opening the discussion about age-related prerequisites as boundary conditions for PF.
Suboptimal human multisensory cue combination
Authors: Arnold Derek H. , Petrie Kirstie , Murray Cailem , Johnston Alan 
Published: March 2019

AbstractInformation from different sensory modalities can interact, shaping what we think we have seen, heard, or otherwise perceived. Such interactions can enhance the precision of perceptual decisions, relative to those based on information from a single sensory modality. Several computational processes could account for such improvements. Slight improvements could arise if decisions are based on multiple independent sensory estimates, as opposed to just one. Still greater improvements could arise if initially independent estimates are summed to form a single integrated code. This hypothetical process has often been described as optimal when it results in bimodal performance consistent with a summation of unimodal estimates weighted in proportion to the precision of each initially independent sensory code. Here we examine cross-modal cue combination for audio-visual temporal rate and spatial location cues. While suggestive of a cross-modal encoding advantage, the degree of facilitation falls short of that predicted by a precision weighted summation process. These data accord with other published observations, and suggest that precision weighted combination is not a general property of human cross-modal perception.
A compact tunable quadrupole lens for brighter and sharper ultra-fast electron diffraction imaging
Authors: Yang Xi , Yu Lihua , Smaluk Victor , Wang Guimei , Hidaka Yoshitreu , Shaftan Timur , Doom Lewis , Padrazo Danny , Li Junjie , Fedurin Mikhail , Wan Weishi , Zhu Yimei 
Published: March 2019

AbstractIn this article, we report our proof-of-principle design and experimental commissioning of a broadly tunable and low-cost transverse focusing lens system for MeV-energy electron beams. The lens system based on electromagnetic (EM) quadrupoles has been built as a part of the existing instrument for ultra-fast electron diffraction (UED) experiments at the Accelerator Test Facility II (ATF-II) at Brookhaven National Laboratory (BNL). We experimentally demonstrated the independent control of the size and divergence of the beam with the charge ranging from 1 to 13 pC. The charge density and divergence of the beam at the sample are the most important factors determining the quality of the Bragg-diffraction image (BDI). By applying the Robust Conjugate Directional Search (RCDS) algorithm for online optimization of the quadrupoles, the transverse beam size can be kept constant down to 75 µm from 1 to 13 pC. The charge density is nearly two orders of magnitude higher than the previously achieved value using a conventional solenoid. Using the BDI method we were able to extract the divergence of the beam in real-time and apply it to the emittance measurement for the first time. Our results agree well with simulations and with the traditional quadrupole scan method. The real-time divergence measurement opens the possibility of online optimization of the beam divergence (<0.2 mrad) at the sample with the increased beam charge. This optimization is crucial for the future development of single-shot ultra-fast electron microscope (UEM). Finally, we demonstrated BDI with significant improvement, up to 3 times higher peak intensity and 2 times sharper Bragg-diffraction peaks at 13 pC. The charge is now limited by the laser power and increasing charge may improve the quality of BDI further. The capability we demonstrated here provides us with opportunities for new sciences using near-parallel, bright and ultrafast electron beams for single-shot imaging, to directly visualize the dynamics of defects and nanostructured materials, or even record molecular movie, which are impossible using present electron-beam technologies.
Mitochondria transfer from mesenchymal stem cells structurally and functionally repairs renal proximal tubular epithelial cells in diabetic nephropathy in vivo
Authors: Konari Naoto , Nagaishi Kanna , Kikuchi Shin , Fujimiya Mineko 
Published: March 2019

AbstractThe underlying therapeutic mechanism of renal tubular epithelium repair of diabetic nephropathy (DN) by bone marrow-derived mesenchymal stem cells (BM-MSCs) has not been fully elucidated. Recently, mitochondria (Mt) transfer was reported as a novel action of BM-MSCs to rescue injured cells. We investigated Mt transfer from systemically administered BM-MSCs to renal proximal tubular epithelial cells (PTECs) in streptozotocin (STZ)-induced diabetic animals. BM-MSCs also transferred their Mt to impaired PTECs when co-cultured in vitro, which suppressed apoptosis of impaired PTECs. Additionally, BM-MSC-derived isolated Mt enhanced the expression of mitochondrial superoxide dismutase 2 and Bcl-2 expression and inhibited reactive oxygen species (ROS) production in vitro. Isolated Mt also inhibited nuclear translocation of PGC-1α and restored the expression of megalin and SGLT2 under high glucose condition (HG) in PTECs. Moreover, isolated Mt directly injected under the renal capsule of STZ rats improved the cellular morphology of STZ-PTECs, and the structure of the tubular basement membrane and brush border in vivo. This study is the first to show Mt transfer from systemically administered BM-MSCs to damaged PTECs in vivo, and the first to investigate mechanisms underlying the potential therapeutic effects of Mt transfer from BM-MSCs in DN.
Development of model web-server for crop variety identification using throughput SNP genotyping data
Authors: Singh Rajender , Iquebal M. A. , Mishra C. N. , Jaiswal Sarika , Kumar Deepender , Raghav Nishu , Paul Surinder , Sheoran Sonia , Sharma Pradeep , Gupta Arun , Tiwari Vinod , Angadi U. B. , Kumar Neeraj , Rai Anil , Singh G. P. , Kumar Dinesh , Tiwari Ratan 
Published: March 2019

AbstractCrop varieties or genotypes of a given species are pivotal for agricultural production and ownership, management and improvement of their germplasm is a great challenge. Its morphological identification requires time, cost and descriptors are often compromised statistically due to phenotypic plasticity. Development of DNA based signature of varieties can overcome these limitations. There is a global need to implement world trade organization (WTO) and intellectual property rights (IPR) guidelines of Plant Breeders Rights (PBR) where DUS (distinctness, uniformity and stability) testing can be supplemented by DNA profile. Universalization and minimization of SNP number without compromising identification accuracy is the major challenge in development of varietal profile by rapid genotype assay. Besides this, there is no server-based approach reducing computational skill with global accessibility of referral phenotypic and genotypic data. We report world’s first model web server for crop variety identification using >350 Indian wheat varieties and Axiom 35 K SNP chip data. Standard filtering and linkage disequilibrium approach were used to develop varietal signature in Linux using HTML, Java, PHP and MySQL with provision of QR code generator to facilitate bar-coding. Phylogenetic tree constructed by selected SNPs confirms six major trait based clusters of varieties and their pedigree. Our user friendly server based tool, VISTa (Variety Identification System of Triticum aestivum) (http://webtom.cabgrid.res.in/vista) can be used in DUS testing having dispute resolution of sovereignty and access benefit sharing (ABS) issues. This model approach can be used in other crops with pan-global level management of crop germplasm in endeavour of crop productivity.
Practical realization of a sub-λ/2 acoustic jet
Authors: Veira Canle Daniel , Kekkonen Tuukka , Mäkinen Joni , Puranen Tuomas , Nieminen Heikki J. , Kuronen Antti , Franssila Sami , Kotiaho Tapio , Salmi Ari , Hæggström Edward 
Published: March 2019

AbstractStudies in optics and acoustics have employed metamaterial lenses to achieve sub-wavelength localization, e.g. a recently introduced concept called ‘acoustojet’ which in simulations localizes acoustic energy to a spot smaller than λ/2. However previous experimental results on the acoustojet have barely reached λ/2-wide localization. Here we show, by simulations and experiments, that a sub-λ/2 wide localization can be achieved by translating the concept of a photonic jet into the acoustic realm. We performed nano- to macroscale molecular dynamics (MD) and finite element method (FEM) simulations as well as macroscale experiments. We demonstrated that by choosing a suitable size cylindrical lens, and by selecting the speed-of-sound ratio between the lens material(s) and the surrounding medium, an acoustic jet (‘acoustic sheet’) is formed with a full width at half maximum (FWHM) less than λ/2. The results show, that the acoustojet approach can be experimentally realized with easy-to-manufacture acoustic lenses at the macroscale. MD simulations demonstrate that the concept can be extended to coherent phonons at nanoscale. Finally, our FEM simulations identify some micrometer size structures that could be realized in practice. Our results may contribute to starting a new era of super resolution acoustic imaging: We foresee that jet generating constructs can be readily manufactured, since suitable material combinations can be found from nanoscale to macroscale. Tight focusing of mechanical energy is highly desirable in e.g. electronics, materials science, medicine, biosciences, and energy harvesting.
The putative tumour suppressor protein Latexin is secreted by prostate luminal cells and is downregulated in malignancy
Authors: Seed Robert I. , Taurozzi Alberto J. , Wilcock Daniel J. , Nappo Giovanna , Erb Holger H. H. , Read Martin L. , Gurney Mark , Archer Leanne K. , Ito Saburo , Rumsby Martin G. , Petrie John L. , Clayton Aled , Maitland Norman J. , Collins Anne T. 
Published: March 2019

AbstractLoss of latexin (LXN) expression negatively correlates with the prognosis of several human cancers. Despite association with numerous processes including haematopoietic stem cell (HSC) fate, inflammation and tumour suppression, a clearly defined biological role for LXN is still lacking. Therefore, we sought to understand LXN expression and function in the normal and malignant prostate to assess its potential as a therapeutic target. Our data demonstrate that LXN is highly expressed in normal prostate luminal cells but downregulated in high Gleason grade cancers. LXN protein is both cytosolic and secreted by prostate cells and expression is directly and potently upregulated by all-trans retinoic acid (atRA). Whilst overexpression of LXN in prostate epithelial basal cells did not affect cell fate, LXN overexpression in the luminal cancer line LNCaP reduced plating efficiency. Transcriptome analysis revealed that LXN overexpression had no direct effects on gene expression but had significant indirect effects on important genes involved in both retinoid metabolism and IFN-associated inflammatory responses. These data highlight a potential role for LXN in retinoid signaling and inflammatory pathways. Investigating the effects of LXN on immune cell function in the tumour microenvironment (TME) may reveal how observed intratumoural loss of LXN affects the prognosis of many adenocarcinomas.
Progesterone elevation on the day of hCG trigger has detrimental effect on live birth rate in low and intermediate ovarian responders, but not in high responders
Authors: Wu Ze , Dong Yunhua , Ma Yanping , Li Yonggang , Li Lei , Lin Na , Li Yunxiu , Zhuan Li , Bai Yun , Luo Xi , Kang Xiaomin 
Published: March 2019

AbstractProgesterone elevation (PE) on the day of hCG trigger is associated with decreased pregnancy outcome in fresh cycles. Evidence for this comes from overall patient estimates that mostly ignore different ovarian responses. To compare the impacts of PE on the day of hCG trigger on live birth rates (LBs) in low, intermediate and high ovarian responders and to explore the cut-off value for PE in different populations according to the ovarian response, we retrospectively analyzed a total of 2,351 patients receiving fresh assisted reproduction technology (ART) transfer cycles with GnRH agonist using a long or short protocol. Trend and multivariate logistic regression analyses were performed to identify the cutoff values of PE and to evaluate the effects of PE on LB rates (LBRs) in different ovarian responders. The study found that PE has a detrimental effect on LBRs in low to intermediate ovarian responders rather than in high responders. The cut-off values for PE were 1.0 ng/mL and 2.0 ng/mL for low and intermediate ovarian responders, respectively. The different associations between PE and LBRs according to ovarian response could more accurately predict the prognosis of the IVF cycle and could be used to optimize the treatment of patients undergoing In Vitro Fertilization (IVF)/ Intracytoplasmic Sperm Injection (ICSI).
Identification and characterization of the lncRNA signature associated with overall survival in patients with neuroblastoma
Authors: Yerukala Sathipati Srinivasulu , Sahu Divya , Huang Hsuan-Cheng , Lin Yenching , Ho Shinn-Ying 
Published: March 2019

AbstractNeuroblastoma (NB) is a commonly occurring cancer among infants and young children. Recently, long non-coding RNAs (lncRNAs) have been using as prognostic biomarkers for therapeutics and interventions in various cancers. Considering the poor survival of NB, the lncRNA-based therapeutic strategies must be improved. This work proposes an overall survival time estimator called SVR-NB to identify the lncRNA signature that is associated with the overall survival of patients with NB. SVR-NB is an optimized support vector regression (SVR)-based method that uses an inheritable bi-objective combinatorial genetic algorithm for feature selection. The dataset of 231 NB patients that contains overall survival information and expression profiles of 783 lncRNAs was used to design and evaluate SVR-NB from the database of gene expression omnibus accession GSE62564. SVR-NB identified a signature of 35 lncRNAs and achieved a mean squared correlation coefficient of 0.85 and a mean absolute error of 0.56 year between the actual and estimated overall survival time using 10-fold cross-validation. Further, we ranked and characterized the 35 lncRNAs according to their contribution towards the estimation accuracy. Functional annotations and co-expression gene analysis of LOC440896, LINC00632, and IGF2-AS revealed the association of co-expressed genes in Kyoto Encyclopedia of Genes and Genomes pathways.