bluuebunny/crossref_metadata_2025_split huggingface dataset:
Whole-brain imaging and characterization ofDrosophilabrains based on one-, two-, and three-photon excitations
Authors: Hsu Kuo-Jen , Lin Yen-Yin , Chiang Ann-Shyn , Chu Shi-Wei 
Published: June 2018

AbstractTo study functional connectome, optical microscopy provides the advantages ofin vivoobservation, molecular specificity, high-speed acquisition, and sub-micrometer spatial resolution. Now, the most complete single-neuron-based anatomical connectome is built uponDrosophila; thus it will be a milestone to achieve whole-brain observation with sub-cellular resolution in livingDrosophila.Surprisingly, two-photon microscopy cannot penetrate through the 200-μm-thick brain, due to the extraordinarily strong aberration/scattering from tracheae. Here we achieve whole-Drosophila-brain observation by degassing the brain or by using three-photon microscopy at 1300-nm, while only the latter providesin vivofeasibility, reduced aberration/scattering and exceptional optical sectioning capability. Furthermore, by comparing one-photon (488-nm), two-photon (920-nm), and three-photon (1300-nm) excitations in the brain, we not only demonstrate first quantitative reduction of both scattering and aberration in trachea-filled tissues, but unravel that the contribution of aberration exceeds scattering at long wavelengths. Our work paves the way toward constructing functional connectome in a livingDrosophila.
CalmAn: An open source tool for scalable Calcium Imaging data Analysis
Authors: Giovannucci Andrea , Friedrich Johannes , Gunn Pat , Kalfon Jérémie , Koay Sue Ann , Taxidis Jiannis , Najafi Farzaneh , Gauthier Jeffrey L. , Zhou Pengcheng , Tank David W. , Chklovskii Dmitri , Pnevmatikakis Eftychios A. 
Published: June 2018

AbstractAdvances in fluorescence microscopy enable monitoring larger brain areasin-vivowith finer time resolution. The resulting data rates require reproducible analysis pipelines that are reliable, fully automated, and scalable to datasets generated over the course of months. Here we present CaImAn, an open-source library for calcium imaging data analysis. CaImAn provides automatic and scalable methods to address problems common to pre-processing, including motion correction, neural activity identification, and registration across different sessions of data collection. It does this while requiring minimal user intervention, with good performance on computers ranging from laptops to high-performance computing clusters. CaImAn is suitable for two-photon and one-photon imaging, and also enables real-time analysis on streaming data. To benchmark the performance of CaImAn we collected a corpus of ground truth annotations from multiple labelers on nine mouse two-photon datasets. We demonstrate that CaImAn achieves near-human performance in detecting locations of active neurons.
Separation and Loss of Centrioles From Primordidal Germ Cells to Mature Oocytes in the Mouse
Authors: Simerly Calvin , Manil-Ségalen Marion , Castro Carlos , Hartnett Carrie , Kong Dong , Verlhac Marie-Hélène , Loncarek Jadranka , Schatten Gerald 
Published: June 2018

AbstractOocytes, including those from mammals, lack centrioles, but neither the mechanism by which mature eggs lose their centrioles nor the exact stage at which centrioles are destroyed during oogenesis is known. To answer questions raised by centriole disappearance during oogenesis, using a transgenic mouse expressing GFP-centrin-2 (GFP CETN2), we traced their presence from e11.5 primordial germ cells (PGCs) through oogenesis and their ultimate dissolution in mature oocytes. We show tightly coupled CETN2 doublets in PGCs, oogonia, and pre-pubertal oocytes. Beginning with follicular recruitment of incompetent germinal vesicle (GV) oocytes, through full oocyte maturation, the CETN2 doublets separate within the pericentriolar material (PCM); concomitantly, a rise in single CETN2 pairs is identified. CETN2 dissolution accelerates following meiosis resumption. Remarkably, a single CETN2 pair is retained in the PCM of most meiotic metaphase-I and -II spindle poles. Partial dissolution of the CETN2 foci occurs even as other centriole markers, like Cep135, a protein necessary for centriole duplication, are maintained at the PCM. Furthermore, live imaging demonstrates that the link between the two centrioles breaks as meiosis resumes and that centriole association with the PCM is progressively lost. Microtubule inhibition shows that centriole dissolution is uncoupled from microtubule dynamics. Thus, centriole doublets, present in early G2-arrested meiotic prophase oocytes, begin partial reduction during follicular recruitment and meiotic resumption, much later than previously thought.
Revealing heterogeneity of brain imaging phenotypes in Alzheimer’s disease based on unsupervised clustering of blood marker profiles
Authors: Martí-Juan Gerard , Sanroma Gerard , Piella Gemma ,   for the Alzheimer’s Disease Neuroimaging Initiative and the Alzheimer’s Disease Metabolomics Consortium
Published: June 2018

AbstractAlzheimer’s disease (AD) affects millions of people and is a major rising problem in health care worldwide. Recent research suggests that AD could have different subtypes, presenting differences in how the disease develops. Characterizing those subtypes could be key to deepen the understanding of this complex disease. In this paper, we used a multivariate, non-supervised clustering method over blood-based markers to find subgroups of patients defined by distinctive blood marker profiles. Our analysis on ADNI database identified 4 possible subgroups, each with a different blood profile. More importantly, we show that subgroups with different profiles have a different relationship between brain phenotypes detected in magnetic resonance imaging and disease condition.
Tandem repeats drive variation of intrinsically disordered regions in budding yeast
Authors: Babokhov Michael , Reinfeld Bradley I. , Hackbarth Kevin , Bentov Yotam , Fuchs Stephen M. 
Published: June 2018

AbstractCopy-number variation in tandem repeat coding regions is more prevalent in eukaryotic genomes than current literature suggests. We have reexamined the genomes of nearly 100 yeast strains looking to map regions of repeat variation. From this analysis we have identified that length variation is highly correlated to intrinsically disordered regions (IDRs). Furthermore, the majority of length variation is associated with tandem repeats. These repetitive regions are rich in homopolymeric amino acid sequences but nearly half of the variation comes from longer-repeating motifs. Comparisons of repeat copy number and sequence between strains of budding yeast as well as closely related fungi suggest selection for and conservation of IDR-related tandem repeats. In some instances, repeat variation has been demonstrated to mediate binding affinity, aggregation, and protein stability. With this analysis, we can identify proteins for which repeat variation may play conserved roles in modulating protein function.
ER-to-Golgi trafficking of procollagen in the absence of large carriers
Authors: McCaughey Janine , Stevenson Nicola L. , Cross Stephen , Stephens David J. 
Published: June 2018

AbstractSecretion and assembly of collagen is fundamental to the function of the extracellular matrix. Defects in the assembly of a collagen matrix lead to pathologies including fibrosis and osteogenesis imperfecta. Owing to the size of fibril-forming procollagen molecules it is assumed that they are transported from the endoplasmic reticulum to the Golgi in specialised large COPII-dependent carriers. Here, analysing endogenous procollagen and a new engineered GFP-tagged form, we show that transport to the Golgi occurs in the absence of large carriers. Large GFP-positive structures are observed occasionally but these are non-dynamic, are not COPII-positive, and label with markers of the ER. We propose a “short-loop” model of COPII-dependent ER-to-Golgi traffic that, while consistent with models of ERGIC-dependent expansion of COPII carriers, does not invoke long-range trafficking of large vesicular structures. Our findings provide an important insight into the process of procollagen trafficking and reveal a short-loop pathway from the ER to the Golgi, without the use of large carriers.SummaryTrafficking of procollagen is essential for normal cell function. Here, imaging of GFP-tagged type I procollagen reveals that it is transported from the endoplasmic reticulum to the Golgi, without the use of large carriers.
Why has Japan’s economy been staggering? A competitiveness perspective
Authors: Parc Jimmyn 
Published: June 2018

Purpose
Once one of the most prominent economies in the world, Japan is currently suffering from economic stagnancy. To revitalize the economy effectively, the core problems of Japan and its economy need to be first identified. This paper aims to understand accurately what the fundamental reasons are for Japan’s current economic stagnancy.

Design/methodology/approach
The generalized double diamond model, which is an extension of Porter’s original diamond model is used to incorporate internationalization which is very important for Asian countries, notably Japan. Furthermore, its competitiveness is compared with neighboring countries that are in competition in the global market.

Findings
Japan’s current economic problems and its slow recovery are because of a lack of globalization vis-à-vis its counterparts in the region, rather than specific macro-economic factors. Hence, further globalization is crucial toward ensuring a further take-off for the economy.

Practical implications
Macro-economic policies may be important but cannot directly improve a nation’s competitiveness. This paper highlights the importance of globalization and concludes that multinational activities are crucial to enhance a nation’s competitiveness in both domestic and international scope.

Originality/value
This paper adopts the concept of national competitiveness to examine the fundamental economic problems of Japan’s slow recovery and stagnancy more comprehensively. In particular, it compares the competitiveness of Japan with its neighbors which are its economic competitors.
Evaluation of a resilience intervention for Filipino displaced survivors of Super Typhoon Haiyan
Authors: Hechanova Maria Regina , Docena Pierce S. , Alampay Liane Peña , Acosta Avegale , Porio Emma E. , Melgar Isabel E. , Berger Rony 
Published: June 2018

Purpose
The purpose of this paper is to evaluate the effect of a community-based resilience intervention for Filipino displaced survivors of Super Typhoon Haiyan.

Design/methodology/approach
The researchers used a quasi-experimental and mixed-method design comparing a treatment group with a control group across three time periods: before, immediately after, and six months after the intervention.

Findings
Results showed significant improvements in survivors’ anxiety scores and resilience scores compared to those who did not undergo the program. However, although there was an increase in adaptive coping of participants immediately after the program, there was a reduction in adaptive coping behaviors for all groups six months after the program. Focus group discussions revealed this might be due to significant environmental challenges among displaced survivors.

Research limitations/implications
A limitation of the study was the lack of randomization and a small sample size due to attrition.

Practical implications
The study highlights the positive effects of culturally adapted group interventions.

Social implications
The results suggest the importance of a systemic approach to enabling the recovery of displaced survivors in developing countries.

Originality/value
This study provides evidence for a resilience intervention developed in a low-middle income country in Southeast Asia.
“I don’t want trouble”
Authors: Longboan Liezel 
Published: June 2018

Purpose
Studies which look at disaster affected people’s use of communications technologies often fail to take into account people’s communication rights in their analyses, particularly their right to freedom of expression. The purpose of this paper is to draw attention to this issue by exploring the link between freedom of expression, community participation and disaster risk reduction in the use of digital feedback channels offered by aid and government agencies in the aftermath of Typhoon Haiyan.

Design/methodology/approach
Ethnographic fieldwork was undertaken in the Philippines between 2014 and 2015 in Tacloban City and Sabay Island, both in the Visayas, which have been affected by Typhoon Haiyan. A total of 101 in-depth interviews were conducted with affected people, local and national officials, community leaders, civil society groups, telecommunications companies and humanitarian agencies.

Findings
The interviews reveal that majority of disaster-affected Filipinos chose not to engage with formal feedback platforms offered by government and aid agencies out of fear of giving critical feedback to those in authority. They were concerned about the possibility of losing their entitlement to aid, of being reprimanded by government officers, and of the threat to their lives and of their loved ones if they expressed criticism to the government’s recovery efforts. Nonetheless, 15 per cent used backchannels while 10 per cent availed of the formal means to express their views about the recovery.

Research limitations/implications
The paper sought to draw links between people’s lack of engagement with the formal feedback mechanisms offered by government and aid agencies in the wake of Haiyan and the restrictive sociopolitical environment in the Philippines. Further research could be undertaken to examine how freedom of expression plays a role in disaster prevention and mitigation. Research into this area could potentially provide concrete steps to help prevent the occurrence of disasters and mitigate their impacts.

Originality/value
Freedom of expression and its place in disaster risk reduction is rarely explored in disaster studies. The paper addresses this oversight by examining the lack of engagement by communities affected by Haiyan with digital feedback channels provided by aid agencies and government. The findings suggest that despite the provisions for community participation in DRR under the Philippine Disaster Law, people are prevented to express criticism and dissent which puts into question the spirit and purpose of the law.
Considerations for future disaster registries
Authors: Petrsoric Lysa , Miller-Archie Sara A. , Welch Alice , Cone James , Farfel Mark 
Published: June 2018

Purpose
The purpose of this paper is to evaluate the effectiveness of a targeted outreach program that referred World Trade Center Health Registry (Registry) enrollees, to specific post-disaster health care available through the World Trade Center Health Program (WTCHP) and evaluate differences in outreach effectiveness based on demographic and health characteristics.

Design/methodology/approach
The Registry’s Treatment Referral Program (TRP) targeted 22,981 enrollees based on symptoms and conditions known to be related to 9/11, reported on a 2011-2012 follow-up survey. A call vendor was utilized for the initial outreach phone call. Enrollees who requested a WTCHP application had follow-up from TRP staff, which typically included 4-6 interactions per enrollee until outreach was completed.

Findings
As of 12/31/2015, the vendor had reached 8,778 (38 percent) of the targeted sample. TRP staff spoke to 6,016 (68 percent) enrollees reached by the vendor, 5,554 (92 percent) of whom requested a WTCHP application, and 2,425 (43 percent) reported having submitted the WTCHP application. Application requests and submissions differed by survivor or responder status, race, income and health symptoms.

Originality/value
Registries created for surveillance and research among disaster-exposed populations provide a unique and effective outreach approach. A dedicated treatment referral unit within a disaster registry is an effective means for conducting post-disaster outreach to a large, diverse sample of exposed individuals.
Managing emotional labor for service employees: an HRM-based approach
Authors: Pandey Jatin 
Published: June 2018

Purpose
This paper aims to describe strategies for human resource managers in dealing with job roles that involve use of emotional labor as part of service delivery.

Design/methodology/approach
The paper suggests improvements in the four processes of recruitment and selection, compensation management, training and development, and performance management to adapt to the needs of employees who perform emotional labor in their job roles.

Findings
The findings show that many interventions can be designed to help these workers; one of the key to these interventions is the recognition of emotional labor as being “affective job demand.” Selecting, rewarding, developing, and evaluating individuals with competencies requires to perform emotional labor can aid service organizations in enhancing their human capital for the long run.

Originality/value
The paper brings forth the importance of emotional labor in organizations and how it can be effectively managed.
Advice for getting the best out of your employees: ignore the problems
Authors: Davis Paul J. 
Published: June 2018

Purpose
The purpose of this paper is to provide HR practitioners with a model to engage employees in organizational improvement initiatives, which is positive, creative, and inclusive.

Design/methodology/approach
The design for this paper is based upon the author’s experience and reading, and the approach is to provide and explain a model with practical examples.

Findings
The author found that the appreciative inquiry method has wide applications with regard to improving organizations of all sizes and industries.

Originality/value
The findings of this paper are valuable to HR, organizational development, change managers, practitioners, and consultants. The model is easy to apply and inexpensive.
Integrated post-disaster medical assistance team scheduling and relief supply distribution
Authors: Wang Shengbin , Liu Feng , Lian Lian , Hong Yuan , Chen Haozhe 
Published: June 2018

Purpose
The purpose of this paper is to solve a post-disaster humanitarian logistics problem in which medical assistance teams are dispatched and the relief supplies are distributed among demand points.

Design/methodology/approach
A mixed integer-programming model and a two-stage hybrid metaheuristic method are developed to solve the problem. Problem instances of various sizes as well as a numerical example based on the 2016 Kyushu Earthquake in Japan are used to test the proposed model and algorithm.

Findings
Computational results based on comparisons with the state-of-the-art commercial software show that the proposed approach can quickly find near-optimal solutions, which is highly desirable in emergency situations.

Research limitations/implications
Real data of the parameters of the model are difficult to obtain. Future collaborations with organizations such as Red Cross and Federal Emergency Management Agency can be extremely helpful in collecting data in humanitarian logistics research.

Practical implications
The proposed model and algorithm can help governments and non-governmental organizations (NGOs) to effectively and efficiently allocate and coordinate different types of humanitarian relief resources, especially when these resources are limited.

Originality/value
This paper is among the first ones to consider both medical team scheduling (routing) and relief aid distribution as decision variables in the humanitarian logistics field. The contributions include developing a mathematical model and a heuristic algorithm, illustrating the model and algorithm using a numerical example, and providing a decision support tool for governments and NGOs to manage the relief resources in disasters.
Value-at-risk performance in emerging and developed countries
Authors: Gaio Luiz Eduardo , Pimenta Júnior Tabajara , Lima Fabiano Guasti , Passos Ivan Carlin , Stefanelli Nelson Oliveira 
Published: June 2018

Purpose
The purpose of this paper is to evaluate the predictive capacity of market risk estimation models in times of financial crises.

Design/methodology/approach
For this, value-at-risk (VaR) valuation models applied to the daily returns of portfolios composed of stock indexes of developed and emerging countries were tested. The Historical Simulation VaR model, multivariate ARCH models (BEKK, VECH and constant conditional correlation), artificial neural networks and copula functions were tested. The data sample refers to the periods of two international financial crises, the Asian Crisis of 1997, and the US Sub Prime Crisis of 2008.

Findings
The results pointed out that the multivariate ARCH models (VECH and BEKK) and Copula-Clayton had similar performance, with good adjustments in 100 percent of the tests. It was not possible to perceive significant differences between the adjustments for developed and emerging countries and of the crisis and normal periods, which was different to what was expected.

Originality/value
Previous studies focus on the estimation of VaR by a group of models. One of the contributions of this paper is to use several forms of estimation.
Financial inclusion and socioeconomic development: gaps and solution
Authors: Rastogi Shailesh , E. Ragabiruntha 
Published: June 2018

Purpose
The purpose of this paper is to identify factors relevant for financial inclusion (FI) and establish a model that shows how these factors lead to economic development (ED) through FI.

Design/methodology/approach
Primary data were collected through structured questionnaire. Out of 350, 311 respondents accurately filled the questionnaire. The data were collected from rural areas of Tamil Nadu. Exploratory factor analysis has been applied to evaluate drivers/factors relevant for FI. Confirmatory factor analysis has been applied to establish reliability and validity of the identified factors. A structural model has been proposed and empirically tested for ED through FI.

Findings
The main findings of the current paper are as follows: online banking (OB), understanding banking services (UBS) and financial literacy (FL) are the drivers of FI; FI can lead to ED, as the proposed model of ED, through FI, is supported in the paper (χ2/degree of freedom and CMIN/degree of freedom are less than 3; GFI and AGFI are more than 0.90 and 0.85, respectively). Behavior of the people, with respect to mode of financial transactions, has changed due to demonetization. (The χ2 test for mode of financial transaction is significant).

Research limitations/implications
The geographical reach of the sample should cover the whole India. The sample should also have equal representation from rural and urban areas.

Practical implications
The identified factors for FI (OB, UBS and FL) should be more focused to bring about better results for FI in India. These factors can lead to a more effective execution of FI initiatives. In addition to this, policy makers can be confident of relying upon FI as a tool for ED.

Originality/value
The identified three drivers for FI have not been explored earlier. In addition to this, ED (through FI) in the form of structural model has also not been tested earlier. Government of India can realign their policies toward FI by using findings of this paper. In addition to increasing the access of formal financial system to masses, more thrust can be given to OB and FL for better results of FI in India.