bluuebunny/crossref_metadata_2025_split huggingface dataset:
PPE39 of the Mycobacterium tuberculosis strain Beijing/K induces Th1-cell polarization through dendritic cell maturation
Authors: Choi Hong-Hee , Kwon Kee Woong , Han Seung Jung , Kang Soon Myung , Choi Eunsol , Kim Ahreum , Cho Sang-Nae , Shin Sung Jae 
Published: August 2019

ABSTRACT
In a previous study, we have identified MTBK_24820, the complete protein form of PPE39 in the hypervirulent Mycobacterium tuberculosis (Mtb) strain Beijing/K by using comparative genomic analysis. PPE39 exhibited vaccine potential against Mtb challenge in a murine model. Thus, in this present study, we characterize PPE39-induced immunological features by investigating the interaction of PPE39 with dendritic cells (DCs). PPE39-treated DCs display reduced dextran uptake and enhanced MHC-I, MHC-II, CD80 and CD86 expression, indicating that this PPE protein induces phenotypic DC maturation. In addition, PPE39-treated DCs produce TNF-α, IL-6 and IL-12p70 to a similar and/or greater extent than lipopolysaccharide-treated DCs in a dose-dependent manner. The activating effect of PPE39 on DCs was mediated by TLR4 through downstream MAPK and NF-κB signaling pathways. Moreover, PPE39-treated DCs promoted naïve CD4+ T-cell proliferation accompanied by remarkable increases of IFN-γ and IL-2 secretion levels, and an increase in the Th1-related transcription factor T-bet but not in Th2-associated expression of GATA-3, suggesting that PPE39 induces Th1-type T-cell responses through DC activation. Collectively, the results indicate that the complete form of PPE39 is a so-far-unknown TLR4 agonist that induces Th1-cell biased immune responses by interacting with DCs.
This article has an associated First Person interview with the first author of the paper.
Myosin V regulates synaptopodin clustering and localization in the dendrites of hippocampal neurons
Authors: Konietzny Anja , González-Gallego Judit , Bär Julia , Perez-Alvarez Alberto , Drakew Alexander , Demmers Jeroen A. A. , Dekkers Dick H. W. , Hammer John A. , Frotscher Michael , Oertner Thomas G. , Wagner Wolfgang , Kneussel Matthias , Mikhaylova Marina 
Published: August 2019

ABSTRACT
The spine apparatus (SA) is an endoplasmic reticulum-related organelle that is present in a subset of dendritic spines in cortical and pyramidal neurons, and plays an important role in Ca2+ homeostasis and dendritic spine plasticity. The protein synaptopodin is essential for the formation of the SA and is widely used as a maker for this organelle. However, it is still unclear which factors contribute to its localization at selected synapses, and how it triggers local SA formation. In this study, we characterized development, localization and mobility of synaptopodin clusters in hippocampal primary neurons, as well as the molecular dynamics within these clusters. Interestingly, synaptopodin at the shaft-associated clusters is less dynamic than at spinous clusters. We identify the actin-based motor proteins myosin V (herein referring to both the myosin Va and Vb forms) and VI as novel interaction partners of synaptopodin, and demonstrate that myosin V is important for the formation and/or maintenance of the SA. We found no evidence of active microtubule-based transport of synaptopodin. Instead, new clusters emerge inside spines, which we interpret as the SA being assembled on-site.
The cytoplasmic C-terminal region of the ATP11C variant determines its localization at the polarized plasma membrane
Authors: Takayama Masahiro , Takatsu Hiroyuki , Hamamoto Asuka , Inoue Hiroki , Naito Tomoki , Nakayama Kazuhisa , Shin Hye-Won 
Published: August 2019

ABSTRACT
ATP11C, a member of the P4-ATPase family, is a major phosphatidylserine (PS)-flippase located at the plasma membrane. ATP11C deficiency causes a defect in B-cell maturation, anemia and hyperbilirubinemia. Although there are several alternatively spliced variants derived from the ATP11C gene, the functional differences between them have not been considered. Here, we compared and characterized three C-terminal spliced forms (we designated as ATP11C-a, ATP11C-b and ATP11C-c), with respect to their expression patterns in cell types and tissues, and their subcellular localizations. We had previously shown that the C-terminus of ATP11C-a is critical for endocytosis upon PKC activation. Here, we found that ATP11C-b and ATP11C-c did not undergo endocytosis upon PKC activation. Importantly, we also found that ATP11C-b localized to a limited region of the plasma membrane in polarized cells, whereas ATP11C-a was distributed on the entire plasma membrane in both polarized and non-polarized cells. Moreover, we successfully identified LLXY residues within the ATP11C-b C-terminus as a critical motif for the polarized localization. These results suggest that the ATP11C-b regulates PS distribution in distinct regions of the plasma membrane in polarized cells.
Price Improvement and Execution Risk in Lit and Dark Markets
Authors: Brolley Michael 
Published: August 2019

Dark pools offer price improvement over displayed quotes, but nondisplayed liquidity implies execution uncertainty. Because investor limit orders also provide price improvement with execution risk, dark pools offer a natural substitute. In a model of informed trading in a market with a displayed limit order book and a dark pool that offers price improvement, higher valuation investors sort into order types with lower execution risk, generating an “immediacy hierarchy.” Dark pool price improvement predicts the order in the hierarchy: a price improvement closer to (farther from) the midquote positions dark orders below (above) limit orders, which improves (worsens) market quality and welfare. A dark pool that is operated by the limit order book is welfare improving, and welfare reduces with an independently operated pool. Because active and passive order flow migrate to the dark pool where price impact occurs only post-trade, price efficiency worsens with any positive level of dark trading. This paper was accepted by Gustavo Manso, finance.
Judgment Error in Lottery Play: When the Hot Hand Meets the Gambler’s Fallacy
Authors: Kong Qingxia , Granic Georg D. , Lambert Nicolas S. , Teo Chung Piaw 
Published: August 2019

We demonstrate that lottery markets can exhibit the “hot-hand” phenomenon, in which past winning numbers tend to have a greater share of the betting proportion in future draws even though past and future events are independent. This is surprising as previous works have instead documented the presence of an opposite effect, the “gambler’s fallacy” in the U.S. lottery market. The current literature also suggests that the gambler’s fallacy prevails when random numbers are generated by mechanical devices, such as in lottery games. We use two sets of naturally occurring data to show that both the gambler’s fallacy and the hot-hand fallacy can exist in different types of lottery games. We then run online experimental studies that mimic lottery games with one, two, or three winning numbers. Our experimental results show that the number of winning prizes impacts behavior. In particular, whereas a single-prize game leads to a strong presence of the gambler’s fallacy, we observe a significant increase in hot-hand behavior in multiple-prize games with two or three winning numbers. This paper was accepted by David Simchi-Levi, behavioral economics.
Jumping the Line, Charitably: Analysis and Remedy of Donor-Priority Rule
Authors: Dai Tinglong , Zheng Ronghuo , Sycara Katia 
Published: August 2019

The ongoing shortage of organs for transplantation has generated an expanding literature on efficient and equitable allocation of the donated cadaveric organs. By contrast, organ donation has been little explored. In this paper, we develop a parsimonious model of organ donation to analyze the welfare consequences of introducing the donor-priority rule, which grants registered organ donors priority in receiving organs should they need transplants in the future. We model an individual’s decision to join the donor registry, which entails a trade-off between abundance of supply, exclusivity of priority, and cost of donating (e.g., psychological burden). Assuming heterogeneity in the cost of donating only, we find the introduction of the donor-priority rule leads to improved social welfare. By incorporating heterogeneity in the likelihood of requiring an organ transplant and in organ quality, we show that, in contrast to the literature, introducing the donor-priority rule can lower social welfare because of unbalanced incentives across different types of individuals. In view of the potentially undesirable social-welfare consequences, we consider a freeze-period remedy, under which an individual is not entitled to a higher queueing priority until after having been on the organ-donor registry for a specified period of time. We show this additional market friction helps rebalance the incentive structure, and in conjunction with the donor-priority rule, can guarantee an increase in social welfare by boosting organ supply without compromising organ quality or inducing excessively high costs of donating. This paper was accepted by Gad Allon, operations management.
How delivery suite co-ordinators create situational awareness in the multidisciplinary team
Authors: Bunford Debra , Hamilton Sharon 
Published: August 2019

Background The role played by the delivery suite co-ordinator in providing safe care has often been overlooked, especially in major maternity policies. There is a paucity of research into this role, how it operates and what is expected by hospitals. Aim To analyse job descriptions to gain an understanding of organisations' expectations of the delivery suite co-ordinator role. Method The study authors undertook qualitative documentary analysis followed by logic modelling. Findings Delivery suite co-ordinators play a vital role in creating a safe clinical environment by fostering situational awareness on the delivery suite. This is achieved through strong leadership skills, which enable the gathering, management and communication of information to and from the multidisciplinary team, and facilitate joint decision-making and planning. Conclusions The delivery suite co-ordinator role requires a different skill set to the clinical midwife. Clinical midwives need support to develop the leadership skills required for this role.
ANÁLISE ESPACIAL E EPIDEMIOLÓGICA DE HEPATITES B E C E ÍNDICE DE DESENVOLVIMENTO HUMANO MUNICIPAL, NO ESTADO DO PARÁ
Authors: Gonçalves Nelson Veiga , Vieira Dayseanne Costa , Miranda Claudia do Socorro Carvalho , Palácios Vera Regina da Cunha Menezes , Costa Simone Beverly Nascimento da , Guedes Juan Andrade , Santos Bruno de Oliveira , Costa Rodrigo Junior Farias da , E Silva Sheila Cristina Martins , Oliveira Rafael Aleixo Coelho de 
Published: August 2019

Considerando as hepatites B e C um grave problema de saúde pública no Brasil, este estudo objetivou analisar a distribuição espacial e socioeconômica destas doenças, nos municípios atendidos por três Centros Regionais de Saúde (CRS), da Secretaria de Saúde Pública do Estado do Pará, de 2010 a 2014. Foi realizado um estudo descritivo e transversal com dados do Ministério da Saúde e do Instituto Brasileiro de Geografia e Estatística. As análises epidemiológicas mostraram que o 1º CRS notificou maior número de casos, sobretudo em 2012 e 2014. O perfil dos indivíduos mais acometidos foi sexo masculino (58,9%), pardos (65,1%), adultos (65,6%), baixa escolaridade (27,3%) e residentes em zona urbana (93,0%). Os fatores de riscos mais significativos nos três CRS foram as doenças sexualmente transmissíveis e uso de medicamentos injetáveis. A análise espacial entre os parâmetros do IDHM e da taxa de incidência mostrou uma autocorrelação inversa entre essas duas variáveis. Foi observado que áreas com IDHM médio e baixo apresentaram altas taxas de incidência. As análises geradas foram eficazes para construir um cenário epidemiológico contextualizado das hepatites B e C. Ressaltamos a necessidade de expansão de programas de controle destas doenças nas áreas estudadas.
ESTUDO SOBRE A INFLUÊNCIA DE VARIÁVEIS METEREOLÓGICAS NOS CASOS DE ACIDENTES POR ANIMAIS PEÇONHENTOS EM LAGES – SC
Authors: Teixeira Schier Daiane , Rafaeli Lemos Mayara , Guimarães Camargo Campos Claudia , Cardoso Josiane Teresinha 
Published: August 2019

A incidência de acidentes por animais peçonhentos se constitui como a segunda causa de notificação epidemiológica no Brasil. Em 2016, do total de casos notificados, 16% correspondem à região sul. Sendo assim, com o objetivo de analisar a possível relação entre os fatores meteorológicos e os casos de notificação de acidentes por animais peçonhentos no município de Lages-SC entre os anos de 2011 a 2017, buscou-se dados a respeito do número de notificações, além de informações sobre a precipitação e temperatura do ar para o período de interesse. As informações relativas aos casos de acidentes por animais peçonhentos foram obtidas pelos indicadores compulsórios do SINAN, enquanto que os dados meteorológicos foram fornecidos pela EPAGRI/CIRAM. O coeficiente de correlação de Pearson foi calculado utilizando-se o software Excel, em que se verificou que os acidentes por animais peçonhentos apresentaram correlação com a temperatura do ar para todos os anos de estudo, sendo o coeficiente mais alto obtido para o ano de 2013 (r = 0,75). Além disso, os casos possuem maiores frequências para janeiro, sente este o mês com temperatura média do ar mais elevada. Dessa forma, torna-se evidente que medidas de cunho preventivo necessitam ser tomadas para diminuir os registros de acidentes por animais peçonhentos, principalmente nos meses de temperaturas mais altas, em que a população possui maior risco de contato com os mesmos e, portanto, deve ser alertada para tomar maiores precauções neste período.
Embedded minimal surfaces of finite topology
Authors: Meeks III William H. , Pérez Joaquín 
Published: August 2019

Abstract
In this paper we prove that a complete, embedded minimal surface
M in

ℝ
3

{\mathbb{R}^{3}}

with finite topology and compact boundary (possibly
empty) is conformally a compact Riemann surface

M
¯

{\overline{M}}

with
boundary punctured in a finite number of interior points and that
M can be represented in terms of meromorphic data on its conformal
completion

M
¯

{\overline{M}}

. In particular, we demonstrate that M is
a minimal surface of finite type and describe how this property
permits a classification of the asymptotic behavior of M.
Modeling of the harmonization method for executing a multi-unit construction project
Authors: Tomczak Michał 
Published: August 2019

Abstract
One of the key problems in managing the realization of a construction project is the selection of appropriate working crews and coordinating their activities in a way that ensures the highest degree of implementation of defined goals (minimizing the project duration and/or reducing downtime and related costs). Most of the existing methods of work harmonization used in construction industry allow obtaining the desired results only in relation to the organization of the processes realization in repetitive linear projects. In case of realization of non-linear construction objects or construction units, it is usually necessary to choose between the reduction of the project implementation time and maintaining the continuity of crews work on the units. It was found that there is a lack in the literature of developed method enabling harmonization of crews’ work, while minimizing the downtime at work and the duration of the entire project taking into account additional constraints, e.g. the need to not exceed the deadlines for the realization of the project stages.
The article presents the concept of a multi-criteria optimization method of harmonizing the execution of non-linear processes of a multi-unit construction project in deterministic conditions. It will enable the reduction of realization time and downtimes in work, taking into account the preferences of the decision maker regarding the relevance of the optimization criteria. A mathematical model for optimizing the selection of crews and order of completion of units in multi-unit construction projects was also developed. In order to present the possibility of usage of the developed concept, an example of the optimal selection of crews and their work schedule was solved and presented. The proposed method may allow for better use of the existing production potential of construction enterprises and ensure synchronization of the crews employed during the work, especially in the case of difficulties in acquiring qualified staff in construction industry.
The GMO Pipeline, Import Bans and Asynchronous Approvals – The Impact on Agricultural Trade
Authors: Pavleska Marija , Kerr William A 
Published: August 2019

Abstract
The divergence in approaches to regulation of genetically modified organisms (GMOs) among countries is a contentious policy issue that has externalities that inhibit trade in agricultural products. One result is that approvals of GMO events is internationally asynchronous. Countries whose approval processes are slower or more stringent often impose import bans or other non-tariff barriers on imports of GM products they have rejected or have yet to approve. These trade barriers have been the subject of considerable investigation. As more and more GMO events are approved in some countries, but not in others, the probability of unintended mingling of GM crops in shipments of non-GM crops increases. Shipments of non-GM crops with a low level presence of non approved GM crops are routinely rejected by importing countries. This growing disruption to trade has not received a great deal of attention. This paper uses information from the GMO research and commercialization pipeline to estimate the potential impact of increases in mingling on international trade flows using a CGE model – GTAP. The results suggest that the growing potential for mingling will have a considerable detrimental impact on trade flows and, hence, tolerance levels should be an important question for multilateral trade negotiations.
Next generation sequencing-guided anti-infective therapy of large liver abscess due to Edwardsiella tarda: a case report
Authors: Zhu Zhong-Zheng , Zhu Jiayi , Zhu Kexuan , Wang Hui , Xu Qing , Bai Chong 
Published: August 2019

AbstractBackgroundLiver abscess is a potentially fatal disease, however identification of its causative pathogen remains challenging, which is crucial for guiding appropriate antibiotic therapy. To the best of our knowledge, the use of plasma next-generation sequencing (NGS) based pathogen analysis in patients with liver abscess has never been reported.Case presentationWe report a case with large liver abscess due toEdwardsiella tardathat was diagnosed by the plasma-based NGS assay within 24 h. After a 2-week single agent anti-infection therapy, the patient completely recovered. A second NGS assay following anti-infection therapy failed to detect the pathogenic DNA. Follow-up magnetic resonance imaging (MRI) of the abdomen on 1 month and 3 months showed a gradually decrease in size of the liver abscess, and the 6-month MRI showed that the abscess disappeared completely.ConclusionsOur data indicated that the plasma NGS test can sensitively and minimal-invasively detect pathogens within a clinically actionable timeframe, and antibiotic alone is an alternative to first-line treatment for large liver abscess with the aid of NGS for accurate detection of the pathogenic bacterial genome.
Range extension and phylogenetic relationships of Akodon josemariarguedasi (Rodentia: Cricetidae) with comments on the aerosus species group
Authors: Ruelas Dennisse , Pacheco Víctor , Jiménez Carlos F. 
Published: August 2019

Abstract
The José María Arguedas’ grass mouse, Akodon josemariarguedasi, is currently known from the central Yungas of Peru, ranging from the right (south) side of the Río Huallaga in Huánuco Department (in the north) to Junín Department (in the south); its phylogenetic affinities are unknown. We collected four specimens from Quintao, Tayacaja, Huancavelica Department allocated to this species, extending the species range 144 km farther south from its previous known distribution; and inferred the phylogenetic relationships of this species within the aerosus species group based on molecular analyses of the mitochondrial cytochrome b gene. We confirm that A. josemariarguedasi is a member of the aerosus species group and it is a sister taxon to Akodon mollis “Ancash” and then to Akodon torques. Also, we found that Akodon kotosh is closely related to Akodon orophilus, and not A. josemariarguedasi. Finally, we confirm that Akodon budini and Akodon siberiae are also members of the aerosus species group.
Crystal structure of (1,4,8,11-tetraazacyclotetradecane)palladium(II) tetracyanoplatinate(II), C14H24N8PdPt
Authors: Ha Kwang 
Published: August 2019

Abstract
C14H24N8PdPt, monoclinic, P21/c (no. 14), a = 9.0747(3) Å, b = 8.3376(3) Å, c = 14.4578(6) Å, β = 123.8260(10)°, V = 908.73(6) Å3, Z = 2, R
gt(F) = 0.0115, wR
ref(F
2) = 0.0235, T = 223 K.
Carbon dot-based fluorometric optical sensors: an overview
Authors: Bagheri Samira , TermehYousefi Amin , Mehrmashhadi Javad 
Published: August 2019

AbstractFluorescent carbon dots (CDs) are a new class of carbon nanomaterials and have demonstrated excellent optical properties, good biocompatibility, great aqueous solubility, low cost, and simple synthesis. Since their discovery, various synthesis methods using different precursors were developed, which were mainly classified as top-down and bottom-up approaches. CDs have presented many applications, and this review article mainly focuses on the development of CD-based fluorescent sensors. The sensing mechanisms, sensor design, and sensing properties to various targets are summarized. Broad ranges of detection, including temperature, pH, DNA, antibiotics, cations, cancer cells, and antibiotics, have been discussed. In addition, the challenges and future directions for CDs as sensing materials are also presented.
GC-MS analysis and biological activities of Thymus vulgaris and Mentha arvensis essential oil
Authors: Ahmad Khan Ashfaq , Shoaib Amjad Muhammad , Saboon  
Published: August 2019

Abstract

Background
Essential oils are chemical products produced by odoriferous glands from a variety of plants. These essential oil have many health benefits i.e. antiseptic, anti-inflammatory and antimicrobial activities. So due to these medicinal properties present study was designed to analyze essential oil of Thymus vulgaris and Mentha arvensis for their chemical composition and biological activities.

Materials and methods
Essential oil from these plants were extracted by hydrodistillation method, and analyzed by GC-MS. To test the microbial activity of these oil disk diffusion method and micro wells method were used. For free radical scavenging DPPH assay was used. However total phenolic content was measured by colorimetric method.

Results
The GC-MS analysis of T. vulgaris oil showed the presence of 47 chemical compounds among which thymol, terpinene, p-cymene and carvacrol were major. However essential oil of M. arvensis showed the presence of 28 constituents, among which the Menthone, Menthol, Isomenthone, Eucalyptol, neo-Menthol, cis-Piperitone oxide, Linalool, Thymol, Limonene, and α-Phellandrene were major. Essential oil from both these plant tested for antimicrobial activity showed that the T. vulgaris oil was effective against seven bacterial strains and the essential oil of M. arvensis was effective against six bacterial strain. The antioxidant activity of both samples by DPPH assay which showed positive result.

Conclusion
As both species showed the presence of active components, positive microbial activities, and antioxidant activity so, research should be carried on for further biological activities of these oil for betterment of living beings.