bluuebunny/crossref_metadata_2025_split huggingface dataset:
Changes in Practitioners’ Attitudes, Perceived Training Needs and Self-Efficacy over the Two-Year Implementation of an Evidence-Based Parenting Program
Authors: Côté Marie-Kim , Gagné Marie-Hélène 
Published: August 2019

Abstract
Background: Evidence-based family support programs such as the Triple P – Positive Parenting Program have the potential to enhance the well-being of children and families. However, they cannot achieve their expected outcomes if insufficient attention is paid to the implementation process. It has been demonstrated that practitioners’ attitudes towards evidence-based programs (EBPs), perceived training needs and self-efficacy for working with parents influence implementation outcomes (e.g., program acceptability, adoption, adherence and sustainability). At the same time, the experience of being involved in the implementation process of an EBP could enhance practitioners’ perceptions of the initiative. This study aimed to assess changes in practitioner’s attitudes, perceived training needs and self-efficacy over a two-year EBP implementation process, in interaction with their appraisal of their organization’s capacity to implement the EPB
Methods: In the province of Quebec, Canada, Triple P was implemented and evaluated in two communities. Ninety-nine practitioners from various organizations completed questionnaires shortly before their training in Triple P and two years later.
Results: Findings show that practitioners who displayed more initial skepticism regarding their organization’s capacity to implement the program reported greater improvements in attitudes over time, while practitioners who showed more optimism at baseline reported a greater decrease in their perceived training needs. Practitioners’ self-efficacy increased moderately regardless of perceived organizational capacity.
Conclusions: These results are encouraging given that more positive perceptions of EBPs could foster the systematic use of these programs in communities, for the potential benefit of a greater number of families.
Obesity, Diabetes and the Risk of Colorectal Adenoma and Cancer
Authors: Soltani Ghodratollah , Poursheikhani Arash , Yassi Maryam , Hayatbakhsh Abdorasool , Kerachian Matin , Kerachian Mohammad Amin 
Published: August 2019

Abstract
Background: Colorectal cancer (CRC) is the fourth most commonly diagnosed gastrointestinal (GI) malignancy and the third leading cause of cancer-related death worldwide. In the current case-control study, an association between diagnosis of CRC, obesity and diabetes was investigated.
Methods: Demographic characteristics, colonoscopy reports, history of drug, smoking, and medical history were collected from patients referred to a colonoscopy unit. The location, size and number of the polyps were recorded during the colonoscopy. Statistically, t-test was conducted for mean comparison for the groups. Pearson's chi-squared test (χ2) was applied to categorize variables. Five classification methods based on the important clinicopathological characteristics such as age, BMI, diabetes, family history of colon cancer was performed to predict the results of colonoscopy.
Results: Overall, 693 patients participated in this study. In the present study, 115 and 515 patients were evaluated for adenoma/adenocarcinoma and normal colonoscopy, respectively. The mean age of patients positive for adenoma or adenocarcinoma were significantly higher than the negative groups (p value <0.001). Incidence of overweight and/or obesity (BMI> 25 kg/m2) were significantly higher in adenoma positive patients as compared to controls (49.9% and 0.9% respectively, p value= 0.04). The results also demonstrated a significant association between suffering from diabetes and having colon adenoma (OR=1.831, 95%CI=1.058–3.169, p value=0.023). The experimental results of 5 classification methods on higher risk factors between colon adenoma and normal colonoscopy data were more than 82% and less than 0.42 for the percentage of classification accuracy and root mean squared error, respectively.
Conclusions: In the current study, the occurrence of obesity measured based on BMI and diabetes in the adenoma positive patient group was significantly higher than the control group although there was no notable association between obesity, diabetes and adenocarcinoma.
Nanopore native RNA sequencing of a human poly(A) transcriptome: RNA extraction, cDNA conversion and direct RNA and cDNA library preparation for Oxford Nanopore
Authors: Workman Rachael E. , Tang Alison D. , Tang Paul S. , Jain Miten , Tyson John R. , Razaghi Roham , Zuzarte Philip C. , Gilpatrick Timothy , Payne Alexander , Quick Joshua , Sadowski Norah , Holmes Nadine , Jesus Jaqueline Goes de , Jones Karen L. , Snutch Terrance P. , Loman Nicholas , Paten Benedict , Loose Matthew , Simpson Jared T. , Olsen Hugh E. , Brooks Angela N. , Akeson Mark , Timp Winston 
Published: August 2019

Abstract
High throughput cDNA sequencing technologies have dramatically advanced our understanding of transcriptome complexity and regulation. However, these methods lose information contained in biological RNA because the copied reads are often short and because modifications are not carried forward in cDNA. We address these limitations using a native poly(A) RNA sequencing strategy developed by Oxford Nanopore Technologies (ONT). Our study focused on poly(A) RNA from the human cell line GM12878, generating 9.9 million aligned sequence reads. These native RNA reads had an aligned N50 length of 1294 bases, and a maximum aligned length of over 21,000 bases. A total of 78,199 high-confidence isoforms were identified by combining long nanopore reads with short higher accuracy Illumina reads. We describe methods for extracting intact RNA, poly-A selection, cDNA conversion for a portion of sample, and library preparation for both direct RNA and cDNA libraries.
MR Imaging Features of Orbital Langerhans Cell Histiocytosis
Authors: wu chunnan , Mu xuetao , Hei yan , lan pengyu , li kuncheng 
Published: August 2019

Abstract
Background: To investigate the magnetic resonance imaging (MRI) features of orbital Langerhans cell histiocytosis (LCH) to improve diagnostic accuracy.
Methods: We retrospectively reviewed clinical manifestations and MRI findings of 23 patients with histopathology-confirmed LCH of the orbit. The findings were evaluated for the following: (a) symptoms, (b) disease duration，(c) location, (d) configuration, (e) margin, (f) MR imaging signal intensity and enhanced performance. Results: Eighteen patients (78%) in our series were male, only five (22%) patients were female, and the mean age at presentation was 6.3 years. The common symptoms include swollen eyelids, exophthalmos, and a palpable mass. Fourteen patients presented with swollen eyelids and/or exophthalmos. Twenty-two cases involved unilateral orbits, and one case involved bilateral orbits. In our study, there was one patient with cough and expectoration, and one patient with polydipsia and polyuria. Lesions were located in the superior or superlateral orbital roof of seventeen patients (74%). Lesions formed masses or irregular shapes. The 12 out of 23 (52.2%)cases appeared heterogeneous isointense and 10 out of 23 (43.5%) cases showed iso-hypointense on T1-weighted imaging, there were 15 out of 23 (65.2%) cases showed hyper-hypointense mixed signals on T2-weighted imaging. 7 cases found patchy hyperintense signal on T1WI, and 11 cases showed markedly hyperintense signal near the edge of lesions on T2WI. After enhancement, 21 out of 23 (91.3%) cases lesions presented marked enhancement at the edges and the surrounding tissues, and with heterogeneous obvious enhancement of the lesion center. Besides, four cases lesions were surrounded by a low circular signal. Conclusion: There were several characteristics MRI features that can provide crucial information for clinicians and improve our understanding and the diagnostic accuracy of the orbital LCH.
MSCs helped reduce scarring in the cornea after fungal infection when combined with anti-fungal treatment
Authors: Zhou Yue , Chen Yuqing , Wang Suiyue , Qin Fangyuan , liya Wang 
Published: August 2019

Abstract
Background

Fungal Keratitis (FK) is an infective keratopathy with extremely high blindness rate. The damaging effect of this disease is not only the destruction of corneal tissue during fungal infection, but also the cornea scar formed during the healing period after infection control, which can also seriously affect a patient's vision. The purpose of the study was to observe the effect of umbilical cord mesenchymal stem cells (uMSCs) on corneal scar formation in FK.

Methods

The FK mouse model was made according to a previously reported method. Natamycin eye drops were used for antifungal treatment 24 hours after modeling. There are four groups involved in the study, including control group, FK group, vehicleinj FK group and uMSCsinj FK group. Mice in uMSCsinj FK group received repeated subconjunctival injections of uMSCs for 3 times at the 1d, 4d and 7d after FK modeling. At 14d, 21d and 28d after trauma, clinical observation, histological examination, second harmonic generation and molecular assays were performed.

Results

The uMSCs topical administration reduced corneal scar formation area and corneal opacity, accompanying with decreased corneal thickness and inflammatory cell infiltration, following down-regulated fibrotic-related factors α-SMA, TGFβ1, CTGF, and COLI and finally inhibited phosphorylation of TGFβ1/Smad2 signaling pathway during FK corneal fibrosis.

Conclusion

The results confirmed that uMSCs can improve corneal opacity during the scar formation stage of FK, and exert anti-inflammatory and anti-fibrotic effects..
Ultrasound-guided quadratus lumborum block for postoperative pain control in patients undergoing unilateral inguinal hernia repair, a comparative study between two approaches
Authors: Fawzy Maher , Nasr Mohamed AR. , Ahmed Abeer , Waheb Mohsen M. , Hussam Ayman M. , Fouad Eman , Aboeldahb Hesham , Saad Dalia , Osman Safinaz , Fahmy Rania Samir , Farid Mohamed 
Published: August 2019

Abstract
Background: early postoperative ambulation and reduction of hospital stay necessitate efficient postoperative analgesia. Quadrates Lumborum Block (QLB) has been described to provide adequate postoperative analgesia after abdominal surgery. This randomized comparative trial was designed to compare the duration of analgesia provided by two different QLB approaches; the posterior QLB (QLB-2) and transmuscular QLB (QLB-3) in patients undergoing surgical repair of unilateral inguinal hernia.
Methods: Forty patients, aged from 18-50 years, ASA physical status I or II, scheduled for unilateral inguinal hernia repair were enrolled. At the end of the surgical procedure and before recovery from general anesthesia, Patients were randomly assigned into two groups to receive either posterior QLB (Group QLB-2) or transmuscular QLB (Group QLB-3) using 20 ml 0.25% bupivacaine. Duration of analgesia, postoperative VAS and postoperative opioid consumption were recorded.
Results: duration of block was significantly longer in QLB-3 group when compared to QLB-2 group (20.1 + 6.2 hours versus 12.0 + 4.8 respectively) with P value of < 0.001. A statistically significant lower VAS score was recorded in QLB-3 group immediately and 12 hrs postoperative. QLB-3 group showed a statistically significant delayed time of first analgesic request and less postoperative morphine consumption with P value of < 0.001 and 0.001 respectively.
Conclusions: Ultrasound guided postsurgical transmuscular approach of QLB (QLB-3) using 20 ml 0.25% bupivacaine produces more postoperative analgesic effect and less postoperative opioid consumption when compared to posterior QLB approach (QLB-2) in patients underwent unilateral inguinal hernia repair under general anesthesia.
Trial Registration: ClinicalTrials.gov identifier: NCT03526731- on 16 May 2018
Keywords: Quadratus lumborum block - Ultrasound-guided transmuscular quadratus lumborum blockade - ultrasound-guided lumbar plexus technique – ultrasound guided transversus abdominus plan block.
Load monitoring on Pilates training: a study protocol for a randomized clinical trial
Authors: Cavina Allysiê P. S. , Junior Eduardo Pizzo , Machado Aryane Flauzino , Biral Taise Mendes , Pastre Carlos Marcelo , Vanderlei Franciele Marques 
Published: August 2019

Abstract
Background: Currently there are campaigns to raise the awareness of the need to practice some physical exercise with several objectives, mainly as a preventive character. From this perspective, we can see the use of the Pilates method as an instrument of therapeutic exercise for the protection and promotion of health. However, despite being popularly performed, there is still no scientific evidence on the standardization of the use of the method and its progression to an adequate prescription of physical training. Therefore, the purpose of the study was to develop a protocol to monitor the progression of Pilates loads daily between the basic, intermediate and advanced levels, as well as to analyze the effect of the method on the psychometric, cardiorespiratory and autonomic parameters. Methods: there will be a total of 36 sessions of Pilates mat for 54 healthy men. In each training session, initially, cardiorespiratory parameters, pain through the Visual Analogue Scale (VAS), and a psychometric questionnaire will be collected for the volunteers. Heart rate (HR), subjective perception of effort (SPE), and RR intervals will be measured during the sessions for subsequent use to analyze the progression of the loads by monitoring the internal training load and heart rate variability (HRV), respectively. At the end of the sessions, cardiorespiratory parameters, the VAS, the psychometric questionnaire will be measured again and the participants will only be released after 15 minutes of rest for the final HR analysis and to re-respond to the PSE scale. Before and after the 36 sessions of training, participants will also be evaluated in relation to psychometric, cardiorespiratory, and autonomic parameters. Discussion: this study deserves to be highlighted as it is a parallel randomized clinical trial with standardization of training, with the purpose of monitoring the prescription of loads of the method, as well as verifying its efficacy in clinical, cardiorespiratory, and autonomic outcomes. The easy reproducibility of the protocol from its description, also improves the study, besides providing support for prescribing the method to the professionals involved.
The efficacy and safety of Shenzhu Guanxin Recipe Granules for treatment of patients with coronary artery disease: protocol for a double-blind, randomized controlled trial
Authors: jin xiao , Biqi Pan , Wu Huanlin , Wu Bingxin , Li Yukai , Wang Xia , Liu Guoqing , Dang Xiaojing , Xu Danping 
Published: August 2019

Abstract
Background

Coronary artery disease (CAD) is one of the most common types of the cardiovascular diseases, previous pilot trials suggested that Traditional Chinese Medicine brought clinical benefits for patients with CAD. We will conduct this trial to determine the efficacy and safety of Shenzhu Guanxin Recipe Granules for treatment of patients with coronary artery disease.

Patients and Methods

This randomized controlled trial recruited 194 patients who were diagnosed CAD by clinical manifestations and examinations, in which coronary computed tomography angiography (CCTA) showed 50-70% of stenosis, with soft or mixed plaque. The included participants were randomly assigned to the case group and control group with a 1:1 allocation ratio; patients in the case group received SGR and usual care, and those in the control group received placebo (6 g/day for 6 months) and usual care. The endpoint of the study included calcium coverage score (CCS), C-Reactive protein (CRP), and the levels of blood lipids, tumor necrosis factor-α (TNF-α), interleukin-1 (IL-1), interleukin-6 (IL-6), and ATP-binding membrane cassette transporter A1 (ABCA1) were calculated before recruiting at6th month. The indicators were Seattle Angina Questionnaire (SAQ) and TCM syndrome questionnaire scores at 0th, 3rd, and 6th months.

Discussion

This clinical trial may provide a reliable evidence regarding the clinical effectiveness and safety of SGR therapy for patients with CAD by clinical manifestations and examinations, in which coronary computed tomography angiography (CCTA) showed 50-70% of stenosis, with soft or mixed plaque.
Comparative study between intrathecal dexmedetomidine and intrathecal magnesium sulfate in prevention of post spinal shivering in uroscopic surgery. (RCT)
Authors: Omar Heba , adel Wessam , Hassan Mohamed Mahmoud , Hassan Amany , Hassan Passaint , Elshall Ahmed , Khalid Dalia , Mostafa Maha , Zarif pierre , hossameldin Mona , Wedad Mai , abdelhamid Bassant Mohamed 
Published: August 2019

Abstract
Background: Hypothermia and shivering are associated common complications after spinal anesthesia especially in uroscopic procedures when large amounts of cold intraluminal irrigating fluids are used. Magnesium sulphate and dexmedetomidine are the most effective adjuvants with least side effects. Our aim of the study is to compare the effect of intrathecal dexmedetomidine versus intrathecal magnesium sulfate in prevention of post spinal shivering.
Methods: This prospective randomized, double-blinded controlled study was conducted at Kasr El-Aini Hospital on 105 patients scheduled for uroscopic surgeries. patients were randomly allocated into three groups using computerized generated random tables, Group C (n=35) received 2.5 ml hyperbaric bupivacaine 0.5% (12.5 mg) +0.5 ml normal saline, Group M (n=35) received 2.5 ml hyperbaric bupivacaine 0.5% (12.5 mg) +25 mg magnesium sulfate in 0.5 ml saline and Group D (n=35) received 2.5 ml hyperbaric bupivacaine 0.5% (12.5mg) + 5 μg dexmedetomidine in 0.5 ml saline. Primary outcomes were the incidence and intensity of shivering. Secondary outcomes were incidence of hypothermia (Temp < 36° C), sedation, the use of meperidine to control shivering and complications, bradycardia, nausea and vomiting.
Results: C group showed statistically significant higher number of total patients who developed shivering (21), patients who developed grade IV shivering (20) and patients who needed meperidine (21) to treat shivering than M group (8,5,5) and D group (5,3,6) which were comparable to each other.
Time needed to give meperidine after giving the block was similar in the three groups. Hypothermia didn’t occur in any patient in the three groups.
The three groups were comparable regarding occurrence of nausea, vomiting, bradycardia & hypotension. All patients of C group, 32 patients in M group and 33 patients in D group had sedation score of 2. 3 patients in M group and 2 patients in D group had a sedation score of 3.
Conclusions: intrathecal injection of dexmedetomidine and magnesium sulfate were both effective in reducing the incidence of post spinal shivering. So, we encourage the use of magnesium sulphate being more physiological, readily available in most operating theatres and much cheaper than dexmedetomidine.
Acupuncture for poststroke hemiplegia focusing on bilateral cerebral connections: study protocol for a randomized controlled trial
Authors: Jiang Lan , Geng Hualei , Lu Mengxin , Du Zhongming , Chen Pei , Han Xiao , Wang Yue , Tang Lixin , Tan Zhongjian , Zhang Hua , Zou Yihuai 
Published: August 2019

Abstract
Background:
Acupuncture is safe and effective for improving the motor function of poststroke hemiplegic patients, but there still exists a certain gap between clinical practice and its neural mechanisms. The cerebral functional reconstruction after unilateral motor pathway injury exhibits a bilateral tendency, however current studies seldom pay attention to it. Hence, based on bilateral cerebral connections, the underlying mechanism of acupuncture in stroke rehabilitation remains research space to date.
Methods: This is a single-center, randomized controlled, paralleled neuroimaging trial, with patients, outcome assessors, and data statisticians blinded. Stroke patients with motor dysfunction meeting the inclusion criteria will be randomly assigned (2:1) to receive either true acupoints treatment or sham acupoints treatment (5 sessions per week for 2 weeks). All the patients will receive conventional treatment and rehabilitation. Participants will undergo the neuroimaging scanning before and after the entire acupuncture treatment. The voxel-mirrored homotopic connectivity (VHMC) will be the primary outcome and the primary effect indicator. The secondary outcomes comprise clinical part and neuroimaging part, which included Fugl-Meyer assessments (FMA), the National Institutes of Health stroke scales (NIHSS), fractional anisotropy (FA) and gray matter volume (GMV). The Needle Sensation Assessment Scale (NSAS) is an additional outcome. The correlation analysis will be explored between the neuroimage indicators and clinical motor assessments.
Discussion:
From the perspective of bilateral connections, this trial will investigate the cerebral influence of acupuncture treatment on poststroke patients with motor dysfunction, promoting application of acupuncture in stroke rehabilitation.
Trial registration:
Chinese Clinical Trials Registry, ChiCTR 1800016263. Registered on 22 May, 2018.
Keywords: Acupuncture, Poststroke hemiplegia, Functional magnetic resonance imaging, Diffusion tensor imaging, Cerebral mechanism, Bilateral cerebral connections
Structural network topology correlates to cognitive impairment and iPTH level in End-stage renal disease patients with peritoneal dialysis
Authors: Wang Longsheng , Zou Liwei , Wu Hanqiu , Shan Yanqi , Zhao Ru , Liu Guiling , Tao Fangbiao , Zheng Suisheng , Gong Xijun 
Published: August 2019

Abstract
Background: The burden of cognitive impairment in patients with end-stage renal disease (ESRD) undergoing peritoneal dialysis has received more attention lately. It associates with hospitalization, mortality and poorer quality of life. We aimed to assess the topological alterations of the brain white matter structural network in ESRD and the correlation between network metrics with Montreal Cognitive Assessment scores and clinical data.
Methods: The study included 25 ESRD patients with secondary hyperparathyroidism (SHPT group), 25 patients without SHPT (Non-SHPT group) and 25 healthy controls (HC group) of comparable age and sex. Montreal Cognitive Assessment was used to assess the cognitive function. The white matter (WM) structural network was constructed by diffusion tensor imaging (DTI) technique, and then we used graph theoretical approaches to detect changes in the global and regional properties of the WM networks in these participants through deterministic tractography method.
Results: ESRD patients showed cognitive impairment compared to HC, and the SHPT patients had lower cognitive scores than the Non-SHPT patients. The global topological organization and local efficiency of the WM network was significantly disrupted in the SHPT but not in the Non-SHPT patients compared with the HC group. Moreover, lower regional efficiency was found in the ESRD patients, mainly distributed in the frontal and parietal cortices. In addition, an association was observed between intact parathyroid hormone (iPTH), shortest path length and cognitive impairment, and the iPTH level was negatively correlated with small-worldness by two indexes, the normalized clustering coefficient and the normalized shortest path length.
Conclusion: The present study indicated that the brain structural connectome in ESRD patients with high iPTH levels was disrupted in association with cognitive impairment and it is a potential connectome-based biomarker for early detection.