bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ecological occurrence and plant regeneration of embryoid of the endangered and endemic plantDysosma versipellisin China
Authors: Tan Xiao-Ming , Zhou Ya-Qin , Wu Pei-Bin , Wei Ying , Yang Shi-Lin , Tang Hong-Zhen 
Published: November 2018

AbstractIn this study, the effective callus culture, somatic embryogenesis, and plant regeneration system ofDysosma versipellis, which is an endangered and endemic plant in China, were established under specific culture conditions. Using theD.versipellisleaves, petioles, and roots as explants, DPS software orthogonal design method and SPSS Duncan’s multiple range test were used to investigate their effects ofD.versipellison callus formation, embryoid induction, and plant regeneration by adding different phytohormones. Results showed that leaves and petioles were the most suitable materials in inducing callus. The effect of phytohormone on callus formation followed the order of 2,4-dichlorophenoxyacetic acid (2,4-D)>thidiazuron (TDZ)> kinetin>naphthylacetic acid (NAA)>2-ip. The best medium for callus formation was MS+2,4-D 1 mg/L+NAA 0.05 mg/L+TDZ 0.5 mg/L+2-ip 1 mg/L. The optimal medium to induce the formation of granular callus embryoid was MS+0.5 mg/L 6-BA+0.1 mg/L NAA, and the induction rate was 71.33%. The embryoid rooting and plant regeneration medium was MS+0.5 mg/L IBA+0.5 mg/L GA3. The optimal medium formula obtained in this study was suitable for the rapid induction of callus, embryoid, and plant regeneration ofD. versipellisunder in vitro culture conditions. Further study on the action mechanism, signal regulation mechanism, and artificial seed production of fungal elicitors affecting the accumulation of podophyllotoxin is important.
CRISPR/Cas9-mediated mutagenesis of phytoene desaturase in diploid and octoploid strawberry
Authors: Wilson Fiona , Harrison Kate , Armitage Andrew D. , Simkin Andrew J. , Harrison Richard J. 
Published: November 2018

ABSTRACTGene editing using CRISPR/Cas9 is a simple and powerful tool for elucidating genetic controls and for crop improvement. We demonstrate use of CRISPR/Cas methodology in diploid Fragaria vesca ssp. vesca ‘Hawaii 4’ and octoploid F. x ananassa ‘Calypso’ by targeting the visible endogenous marker gene PDS (phytoene desaturase). Agrobacterium-mediated transformation of leaf and petiole explants was used for efficient stable integration of constructs expressing plant codon-optimised Cas9 and single guide sequences under control of either the Arabidopsis U6-26 consensus promoter and terminator or Fragaria vesca U6III regulatory sequences. More than 80% (‘Hawaii 4’) and 50% (‘Calypso’) putative transgenic shoot lines exhibited mutant phenotypes. Of mutant shoot lines selected for molecular analysis, approximately 55% (‘Calypso’) and 75% (‘Hawaii 4’) included albino regenerants with bi-allelic target sequence variants. Our results indicate the PDS gene is functionally diploid in ‘Calypso’ and clearly demonstrate that CRISPR/Cas9 can be used to edit single copy genes at high frequency within the genome of the diploid and the same target in octoploid strawberry.
Spindle checkpoint silencing at kinetochores with submaximal microtubule occupancy
Authors: Etemad Banafsheh , Vertesy Abel , Kuijt Timo E.F. , Sacristan Carlos , van Oudenaarden Alexander , Kops Geert J.P.L. 
Published: November 2018

SummaryThe spindle assembly checkpoint (SAC) ensures proper chromosome segregation by monitoring kinetochore-microtubule interactions. SAC proteins are shed from kinetochores once stable attachments are achieved. Human kinetochores consist of hundreds of SAC protein recruitment modules and bind up to 20 microtubules, raising the question how the SAC responds to intermediate attachment states. We show that the ‘RZZS-MAD1/2’ module of the SAC is removed from kinetochores at low microtubule occupancy and remains absent at higher occupancies, while the ‘BUB1/R1’ module is retained at substantial levels irrespective of attachment states. Artificially tuning the affinity of kinetochores for microtubules further shows that ~50% occupancy is sufficient to shed MAD2 and silence the SAC. Kinetochores thus responds as a single unit to shut down SAC signaling at submaximal occupancy states, but retains one SAC module. This may ensure continued SAC silencing on kinetochores with fluctuating occupancy states while maintaining the ability for fast SAC re-activation.
Stemming the tide of work-related stress: it’s not rocket science, it’s neuroscience
Authors: Chase Maria Alexis 
Published: November 2018

Purpose
This paper aims to illuminate how the findings from neuroscience, translate into simple, elegant, and easily applied tools which offer highly effective strategies to help stem the rising tide of stress in global work-forces.

Design/methodology/approach
Focusing on how current working practices inadvertently activate the brains primal survival mechanism, this paper details the brain’s main organizing principle and the negative effect this has on performance and productivity.

Findings
Exemplifying why, how, and when this automated stress response is triggered, the paper suggests practical, real-world solutions to overcome the primal fight, flight, and freeze mechanism. As this mechanism is responsible for the majority of work-related stress, it discusses the brains capacity to re-wire and change behavioral responses to stress at an individual and organizational level

Originality/value
This study seeks to introduce the concept of aligning work place practices with biological processes. Hoping to open a debate around shifting cultural perceptions of what constitutes the real well-being of work forces, it offers a unique and fresh perspective into the machinery of the mind.
Competing values in Asian business: evidence from India and Dubai
Authors: Rao Indu 
Published: November 2018

Purpose
The purpose of this paper is to highlight the fact that investors in the Asian region are shifting their investments from one country to another, in this case, from India to Dubai, in the real estate and infrastructure industry. While countries compete to get investments, competing “values” at the workplace may also influence in attracting the investments. This paper makes use of competing values framework (CVF) to understand this phenomenon and to provide research evidence about differences in workplace cultures in India and Dubai. It is proposed that differences in workplace cultures, besides other non-cultural factors, may influence this phenomenon of shifting of investments between the two countries.

Design/methodology/approach
It is an inductive study to investigate why investors are shifting investments from India to Dubai in the real estate and infrastructure industry. This paper further explores literature to support our claim that workplace cultural differences may be responsible for the shifting investments. Next, this paper identifies the instrument called organizational culture assessment instrument using CVF to collect data and plot the cultural profiles at the two country sites.

Findings
The findings suggest that workplace cultures in the two country locations are different and could be a reason for Indians to shift their investments to Dubai in the real estate and infrastructure sector. There are both cultural and non-cultural factors, which are responsible for the shift in global investments.

Research limitations/implications
The study has several research implications. It highlights the possibility of a shift in global investments because of cultural and non-cultural differences at the workplace. Specifically, it provides evidence that workplace cultures are different in the two countries and could play a role in the competitiveness of firm and countries. This finding has implications for research in the fields of both strategy and international business.However, this is a preliminary study to explore a recent phenomenon and uses data from only one organization in two countries. Therefore, this paper accepts this as a limitation; however, it creates a potential for further exploration in many directions for future research.

Practical implications
Managers in multinational firms have to deal with subsidiaries in different countries with different cultures. While culture is not traditionally considered an important factor, the study highlights that it may have far-reaching influences on financial decisions. Therefore, managers need to understand cultures and create strategies to deal with diverse cultures.

Originality/value
It is perhaps the first attempt to investigate the workplace culture across India and Dubai in the real estate and infrastructure industry through empirical evidence. Further, in the context of shifting global investments across the two countries, it highlights the importance of workplace cultures towards economic and financial implications for countries in the Asian subcontinent.
Contextual layers of service experience in professional business services
Authors: Still Johanna , Komulainen Hanna , Nätti Satu 
Published: November 2018

Purpose
This study provides us with new knowledge in the form of conceptual framework of the contextual layers of service experience within professional business services. This study aims to answer the following questions: What kinds of contextual layers can be identified influencing service experience? How specific characteristics of professional service context may influence customer experience at these different layers?

Design/methodology/approach
The framework is based on extensive literature review considering research in the fields of service and relationship perspectives, likewise professional services.

Findings
The framework is based on extensive literature review in the fields of service and relationship perspectives, likewise professional services.

Originality/value
Only a limited number of studies seem to address the highly topical context of professional/knowledge-intensive business services and relationships. The authors tie the discussion concerning different contextual layers of service experience to this specific operating context with the aim of identifying their importance and influence in service experience. Related to this context, this study highlights the importance of understanding role of individuals in service experience, rarely emphasized in B2B dyadic setting. The framework also contributes to current discussion regarding service experience and “zooms in” to the context and its detailed levels.
Placing social capital in business networks: conceptualisation and research agenda
Authors: Bondeli Julia V. , Havenvid Malena Ingemansson , Solli-Sæther Hans 
Published: November 2018

Purpose
This paper aims to refine conceptual treatment of the social facet in business relationships and reinforce its significance in the industrial marketing and purchasing (IMP) research tradition by integrating the concept of social capital in its original interpretation into the actor-resource-activity (ARA) model.

Design/methodology/approach
The paper begins by indicating some typical conceptual challenges associated with application of social capital in IMP. This is followed by a conceptual clarification that explores the origin and the essence of social capital in economic sociology. Finally, the paper proposes integrating social capital in its original interpretation into IMP’s ARA model and presents four propositions on how social capital is created in interaction between business actors.

Findings
The paper shows how bridging Bourdieu’s theory of social capital with the IMP approach may solve the identified conceptual challenges. This paper’s main contribution is a cyclical model depicting how social capital is created in business networks. It is integrated into the ARA model and designed specifically for studying the social facet of business relationships.

Research limitations/implications
The paper is expected to aid IMP researchers in empirical contexts where the social component in business relationships is particularly prominent. As such, the novel approach presented could be used to further understand how social exchange processes are related to relationship governance, relationship initiation and development.

Originality/value
The proposed model shows how social capital is generated through the dynamic interplay in the social facets of actor, activity and resource dimensions, emphasising its creation dynamics. The model integrates insights from the classic works in economic sociology to strengthen the social side of IMP’s socioeconomic interface and is intended to be used as a tool for empirical application.
Algorithm applied in corporate sustainability
Authors: Barcellos de Paula Luciano , Gil-Lafuente Anna Maria 
Published: November 2018

PurposeScientific studies indicate that stakeholder’s engagement is a key factor for the creation of sustainable value in companies. This paper aims to evaluate the sustainability of a sports company and propose a tool to prioritize the relevant issues that this company should consider in its operations.Design/methodology/approachStakeholder theory and Global Reporting Initiative (GRI) are considered, and, from the Fuzzy Logic, the paper proposes a decision-making tool to establish the relevant issues. The “Experton Method” is used in this paper.FindingsThe algorithm used can help decision makers in processes that include the stakeholder’s opinions. In this case, a tool that treats qualitative data in a reliable manner is required. The methodology used allowed knowing the stakeholders expectations and to define priorities in sustainability issues. The results were captured in a materiality matrix. The manuscript concludes that including stakeholders in the decision-making process, the company generates trust and legitimacy of its stakeholders. This participatory approach and the use of algorithm help companies in materiality assessment, definition of priority issues and management of resources.Practical implicationsIn terms of managerial implications, this paper presents a useful tool that can help entrepreneurs in the decision-making to manage their suppliers. Using an algorithm of fuzzy logic applied in the supply chain management, it is indicated how to set priorities to build a consistent corporate social responsibility (CSR) plan to achieve corporation success. This methodology allows reducing subjectivity; it generates greater precision and decreases the risk in decision-making. At the same time, it promotes dialogue and collaboration among stakeholders to create value for stakeholders and CSR, and collaborate to have a responsible company performance. Furthermore, this paper provides theoretical applications in terms of the literature review on corporate sustainability, indicating that companies must consider the stakeholder’s engagement in its strategies. Based on the bibliometric study, there are knowledge gaps on the subject. For these reasons, an important contribution is observed at the academic level that allows expanding the frontier of knowledge on the subject.Originality/valueA tool for decision-making is presented with great utility for entrepreneurs in the processes of dialogue and stakeholder’s engagement, being a contribution for the creation of sustainable value. In addition, there is an important scientific contribution because the paper identifies in the literature the knowledge gaps on the subject.
The effect of tempeh intake on spatial memory in prediabetic rats
Authors: Ayuningtyas Annisa , Murbawani Etisa Adi , Nuryanto Nuryanto 
Published: November 2018

Purpose
Prediabetes can affect the central nervous system and lead to changes including impaired cognition and spatial memory. Isoflavones, especially genistein and daidzein, can increase spatial memory. Tempeh is a soy product with high levels of genistein and daidzein. The purpose of this paper is to determine the effect of tempeh on spatial memory of prediabetic Wistar rat.

Design/methodology/approach
A pre-and post-test randomised control group design was used. In total, 15 male Wistar rats were divided into three equal groups, Treatment 1 (T1), Treatment 2 (T2) and Treatment 3 (T3). All rats were injected with alloxan (120 mg/Kg body weight) to induce prediabetes. T1 was maintained on a diet of standard food for 14 days. T2 and T3 were given 9 g/200 g body weight and 18 g/200 g body weight of tempeh, respectively, for 14 days. The Morris water maze (MWM) test was used to test spatial memory.

Findings
While there were no statistically significant differences between the treatment groups, there was a difference with the tempeh-treated rats improving their spatial memory following tempeh ingestion with a decrease in the travel time to negotiate the MWM [T1(−8.36), T2 (−5.48) and T3 (3.66)]. Differences of travel time of the three groups was not significant (p = 0.677).

Originality/value
The result of the study indicates that tempeh may act as a functional food in reducing cognitive impairment associated with prediabetes and diabetes. However, as the result is not statistically significant, further research using animal models and human studies is warranted.
Ageing in an herbaceous plant: Increases in mortality and decreases in physiology and seed mass
Authors: Quarles Brandie M. , Roach Deborah Ann 
Published: November 2018

Abstract

Little is known about plant age‐dependent trait expression and how environmental conditions might affect ageing in the wild. This study evaluates age variation in multiple traits of a short‐lived perennial herb using a manipulative field experimental design.

Two different‐aged cohorts were followed in a field plot for over a year to evaluate trait expression in response to a competition treatment and seasonal stress. Traits measured included size, mortality, reproduction, and physiology, including photosynthetic efficiency and chlorophyll content (SPAD). We hypothesized that the stress of competition and seasonal changes would accentuate age‐dependent trait declines in older plants.

The results highlight consistent age differences in plant size, mortality, and seed size with older plants being smaller, more likely to die, and producing smaller seeds. Some of the ageing declines were sensitive to environmental conditions such that it was only during certain seasons when older plants had higher mortality, lower photosynthetic efficiency, and lower chlorophyll content than young plants. Age‐dependent trait expression also varied in response to competition such that age differences in size were only present in the “no competition treatment,” and old individuals in the competition treatment had a higher mortality than all other age‐environment combinations.

Synthesis. These findings show that ageing in plants is a complex phenotype where declines in traits are uncoordinated and can be, but are not always, sensitive to environmental conditions. This study shows age‐dependent maternal effects on offspring quality which, together with the decline in performance of older individuals, may have impacts on an individual’s fitness and on natural population demography.
Reconceptualizing developmental language disorder as a spectrum disorder: issues and evidence
Authors: Lancaster Hope S. , Camarata Stephen 
Published: November 2018

AbstractBackgroundThere is considerable variability in the presentation of developmental language disorder (DLD). Disagreement amongst professionals about how to characterize and interpret the variability complicates both the research on understanding the nature of DLD and the best clinical framework for diagnosing and treating children with DLD. We describe and statistically examine three primary possible models for characterizing the variability in presentation in DLD: predictable subtypes; individual differences; and continuum/spectrum.AimsTo test these three models of DLD in a population‐based sample using two distinct types of cluster analyses.Methods & ProceduresThis study included children with DLD (n = 505) from the US Epidemiological Study of Language Impairment database. All available language and cognitive measures were included. Two cluster methods were used: Ward's method and K‐means. Optimal cluster sizes were selected using Bayesian information criteria (BIC). Bootstrapping and permutation methods were used to evaluate randomness of clustering.Outcomes & ResultsBoth clustering analyses yielded more than 10 clusters, and the clusters did not have spatial distinction: many of these clusters were not clinically interpretable. However, tests of random clustering revealed that the cluster solutions obtained did not arise from random aggregation.Conclusions & ImplicationsNon‐random clustering coupled with a large number of non‐interpretable subtypes provides empirical support for the continuum/spectrum and individual differences models. Although there was substantial support for the continuum/spectrum model and weaker support for the individual differences model, additional research testing these models should be completed. Based on these results, clinicians working with children with DLD should focus on creating treatment plans that address the severity of functioning rather than seeking to identify and treat distinct subtypes. Additional consideration should be given to reconceptualizing DLD as a spectrum condition.
Attribute Specific Impacts of Stated Non‐Attendance in Choice Experiments
Authors: Mohanty Suva Kanta , Balcombe Kelvin , Bennett Richard , Nocella Giuseppe , Fraser Iain 
Published: November 2018

AbstractIn this paper, we generalise existing approaches to the treatment of stated attribute non‐attendance data in discrete choice experiments by allowing attribute specific impacts. We implement this approach by employing an extended hierarchical Bayes logit model specification. To illustrate this approach, we consider data collected to examine Indian consumers’ preferences for traditional aromatic rice varieties. Our results regarding stated attribute non‐attendance reveal that, our new approach shrinks marginal utilities of non‐attenders substantially compared to stated attenders, with significant differences in the shrinkage between some of the attributes. In addition, our results reveal the way in which non‐attendance of attributes interact with each other and the impact that this has on the distribution of willingness to pay estimates.
Evaluation of Nile Red‐Loaded Mesoporous Silica Nanoparticles for Developing Water‐Soaked Fingerprints on Thermal Paper
Authors: Wang Wenjing , Xing Jinfeng , Ge Zhiqiang 
Published: November 2018

AbstractNile red has been an alternative reagent for detecting latent fingerprints on wetted substrates. However, the presence of methanol in nile red solution could make injury to handlers and destroy the traces on surfaces, such as texts on thermal papers. A novel small particle reagent formulation constituting of mesoporous silica nanoparticles (MSNs) based on nile red was prepared to overcome the problem. Compared with the conventional reagents Oil Red O or nile red solution, the nile red‐loaded MSNs are highly selective to lipid residues of fingerprints and showed a greater ability to develop clear, sharp, and detailed fingerprints on thermal papers after these were immersed in water. In addition, it can retain texts on the thermal papers well and use only water as a solvent. These suggested that nile red‐loaded MSNs are a safe, efficient, and convenient method to develop latent fingerprints on wide range of substrates of forensic importance.