bluuebunny/crossref_metadata_2025_split huggingface dataset:
Penerapan Akuntansi Persediaan Barang Dagang pada UD Prapatan Motor Kudus
Authors: Rakhmawati Ita 
Published: September 2019

<p><em>Merchandise inventory is one account that has significant values </em><em></em><em>and functions. The amount of inventory must not be too much because it will affect to liquidity. However, it should not be too small because it will eliminate potential of revenue from customers. This research aim to whether the accounting treatment of good inventory methods on Prapatan Motor Entreprise. </em><em>This research uses qualitative approach, The types of data collected are primary and secondary data. This study related of recording, assessment, and reporting of finished goods inventory . This study concludes</em><em> Prapatan Motor Entreprise have execute an accounting inventory process. The company use physical recording methods and average methods on inventory assesment.on the end of accounting period, Inventory present on financial position and cost of production report. But the company don’t have inventory control card so must stock opname periodically.</em></p><p> </p>
Application of Fluorescent Markers to Determine the Formation Fluid Inflow After MFrac
Authors: Guryanov Andrey , Gazizov Ruslan , Medvedev Evgeny , Ovchinnikov Kirill , Buzin Pavel , Katashov Alexander 
Published: September 2019

Abstract
The primary objective of the technical development and underlying principles described in this article is the creation of physicochemical principles, of which the practical implementation allows users to quickly and accurately conduct production logging of horizontal wells after conducting multi-stage hydraulic fracturing.
The main physical phenomenon applied in the described method is the fluorescence of polymer microspheres – marker-reporters ranging in size from several hundred nanometers to several microns and containing quantum dots. Marker-reporters pass from synthesis and injection of proppant/sand into the polymer shell at our company's production facilities to high-precision instrumental determination of their concentration in formation fluid samples using flow cytofluorometry method in the laboratory. This method includes the following stages:
Synthesis of marker-reporters containing quantum dots Preparation of polymer-coated proppant / sand with markers Injection of the marked polymer-coated proppant / sand into the well during MFrac, followed by formation fluid filtration through it Formation fluid sampling Sample preparation for obtaining samples to be analyzed with the flow cytometer Determination of marker-reporter concentrations in the samples by flow cytofluorometry data processing, also with our corporate software based on machine- learning principles
All the stages mentioned above are constantly being improved and optimized. The description of each stage of the relevant technological process is described below with a "historical reference" to the technological development behind it. The characteristics of the marked polymer-coated proppant Geosplit are also provided herein.
Study of the Prospects for Gas-Containing Terrigenous Deposits of the Middle and Lower Jurassic on One of the Areas within the Afghan-Tajik Depression
Authors: Tillyabaev Muzafar , Abidov Khurshid , Klevitskiy Alexandr , Levin Dmitry , Shamkolovich Galina 
Published: September 2019

Abstract
Given the current degree of exploration maturity within the Afghan-Tajik Depression, much of which lies within the southeastern Republic of Uzbekistan, there is a vital need to conduct geological studies of the Lower to Middle Jurassic strata and to elaborate a geological exploration program that will achieve the most comprehensive substantiation of hydrocarbon potential associated with these sediments. Since none of the prospecting and appraisal wells has reached the deep-seated Lower to Middle Jurassic sediments in the region of interest, an in-depth study of available seismic data aimed at estimating their hydrocarbon potential becomes a matter of high priority. This paper discusses the studies conducted in one of 3D seismic areas on the Lower to Middle Jurassic sediments. Porosity data from analogous fields were statistically analysed for the purpose of estimating hydrocarbon resources. This step helped substantiate volumetric parameters even using extremely scarce actual information. The completed studies have resulted in recommendations for drilling prospecting wells into the target Lower to Middle Jurassic strata as well as predicting the depth of burial at which producing sandstones occur.
Transcriptomic and evolutionary analysis of the mechanisms by which P. argentatum, a rubber producing perennial, responds to drought
Authors: Nelson Andrew D. L. , Ponciano Grisel , McMahan Colleen , Ilut Daniel C. , Pugh N. Ace , Elshikha Diaa Eldin , Hunsaker Douglas J. , Pauli Duke 
Published: September 2019

Abstract
Background Guayule (Parthenium argentatum Gray) is a drought tolerant, rubber producing perennial shrub native to northern Mexico and the US Southwest. Hevea brasiliensis, currently the world’s only source of natural rubber, is grown as a monoculture, leaving it vulnerable to both biotic and abiotic stressors. Isolation of rubber from guayule occurs by mechanical harvesting of the entire plant. It has been reported that environmental conditions leading up to harvest have a profound impact on rubber yield. The link between rubber biosynthesis and drought, a common environmental condition in guayule’s native habitat, is currently unclear. Results We took a transcriptomic and comparative genomic approach to determine how drought impacts rubber biosynthesis in guayule. We compared transcriptional profiles of stem tissue, the location of guayule rubber biosynthesis, collected from field-grown plants subjected to water-deficit (drought) and well-watered (control) conditions. Plants subjected to the imposed drought conditions displayed an increase in production of transcripts associated with defense responses and water homeostasis, and a decrease in transcripts associated with rubber biosynthesis. An evolutionary and comparative analysis of stress-response transcripts suggests that more anciently duplicated transcripts shared among the Asteraceae, rather than recently derived duplicates, are contributing to the drought response observed in guayule. In addition, we identified several deeply conserved long non-coding RNAs (lncRNAs) containing microRNA binding motifs. One lncRNA in particular, with origins at the base of Asteraceae, may be regulating the vegetative to reproductive transition observed in water-stressed guayule by acting as a miRNA sponge for miR166. Conclusions These data represent the first genomic analyses of how guayule responds to drought like conditions in agricultural production settings. We identified an inverse relationship between stress-responsive transcripts and those associated with precursor pathways to rubber biosynthesis suggesting a physiological trade-off between maintaining homeostasis and plant productivity. We also identify a number of regulators of abiotic responses, including transcription factors and lncRNAs, that are strong candidates for future projects aimed at modulating rubber biosynthesis under water-limiting conditions common to guayules’ native production environment.
Scalable methanol-free production of recombinant glucuronoyl esterase in Pichia pastoris
Authors: Conacher Cleo G. , García-Aparicio María , Coetzee Gerhardt , Zyl W.H. (Emile) van , Görgens Johann F. 
Published: September 2019

Abstract
Objective: Glucuronoyl esterase (GE) is an emerging enzyme that improves fractionation of lignin-carbohydrate complexes. However, the commercial availability of GE is limited, which hinders the research of GE-based bioprocesses for its industrial application in lignocellulose biorefineries. This study evaluated a workable, cost-effective, and commercially scalable production strategy to improve the ease of GE-based research. This strategy consisted of a constitutive and methanol-free enzyme production step coupled with a two-step filtration process. The aim was to determine if this strategy can yield copious amounts of GE, by secretion into the extracellular medium with an acceptable purity that could allow its direct application. This approach was further validated for cellobiose dehydrogenase, another emerging lignocellulose degrading enzyme which is scarcely available at high cost. Results: The secreted recombinant enzymes were functionally produced in excess of levels previously reported for constitutive production (1489-2780 mg.L -1 ), and were secreted at moderate to high percentages of the total extracellular protein (51-94 %). The constant glycerol feed, implemented during fed-batch fermentation, lead to a decline in growth rate and plateaued productivity. Tangential flow ultrafiltration was used to concentrate cell-free enzyme extracts 5-6-fold, reaching enzyme activity levels (1020-202 U.L -1 ) that could allow their direct application.
Comparative analysis of IGFBP-3 gene sequence in Egyptian sheep, cattle, and buffalo
Authors: Saleh Ahmed Abdel-kader , Rashad Amr M.A. , Hassanine Nada. N.A.M. , Sharaby Mahmoud A. , Zhao Yongju 
Published: September 2019

Abstract
Objective: A total of 205 animals from four Egyptian livestock species; cattle (n=18), buffaloes (n=12), sheep (n=150) and goats (n=25) were used in this study to detect polymorphism and perform comparative analysis for IGFBP-3 gene using DNA sequencing and (PCR-RFLP).
Results: The amplified fragments were found to be of length 654 bp in sheep, 651bp in cattle and 655 bp in buffalo. For Falahy goats, PCR was performed to amplify a 316 bp fragment from exon 2 of the IGFBP-3 gene. The digestion of 654 bp with HaeIII restriction enzyme yielded a single restriction pattern for goats, while for cattle, 3 genotypes were identified; (AA),(AB), and (BB). Moreover, for buffalo one genotype (AA) only was found with HaeIII and TaqI restriction enzymes, separately. Also, the digestion profile for goats with HaeIII revealed one pattern only. Nucleotide sequencing of the amplified fragments of IGFBP-3 gene in sheep, cattle, buffalo, and goat was submitted to the NCBI GenBank (Accession no. MG738671.1, MG738673.1, MG738674.1, and MG738672.1, respectively). The nucleotide sequencing analysis indicated similarity percentages in IGFBP-3 gene fragments of 88.54, 89.63 and 95.06 % between ''sheep and cattle '', ''sheep and buffalo '', and ''cattle and buffalo'', respectively.
A protocol for Epon-embedding-based correlative super-resolution light and electron microscopy
Authors: Xu Pingyong , Xu Tao , Zhang Mingshu , Fu Zhifei , Peng Dingming , Xue Fudong , Zhang Rui , He Wenting 
Published: September 2019

Abstract
This protocol describes a detailed step-by-step sample preparation procedure for Epon based post-embedding correlative super-resolution light and electron microscopy (SR-CLEM). The newly developed fluorescent protein, mEosEM, is the core in this protocol. The advantage of this method is to simultaneously obtain high-quality LM and EM images from the same ultrathin section of Epon-embedded sample after conventional chemical fixation.
Powdery and Downy mildew infections regulates a cascade of defense-responsive genes in Vitis Vinifera
Authors: Goyal Neetu , Bhatia Garima , Garewal Naina , Upadhyay Anuradha , Singh Kashmir 
Published: September 2019

Abstract
Background: Grape cultivation is severely affected by powdery mildew and downy mildew worldwide leading to significant losses in productivity and quality. To control such diseases in an environment friendly way, it is essential to understand the molecular mechanisms of fungal resistance in grapes. When the plant is infected with pathogen, various defense mechanisms are activated in plants at molecular level, the ultimate aim of all is to provide immunity against infection. Results: In our study, we tried to understand the Salicylic acid (SA) mediated pathway of ETI through a cascade of defense molecules ultimately leading to activation and expression of PR proteins that provides SAR in plants. We performed genome-wide identification of defensive molecules expressed during PM and DM infection in grapevine. Consequently, we identified 2, 4, 7, 4, 2, 1, 20 and 7 differentially expressed PM-responsive defensive genes ( NBS-LRR, EDS1, NDR1, NPR, PAD4, RAR1, TFs and PR ) and 28, 2, 5, 4, 1, 39 and 19 differentially expressed DM-responsive defensive genes ( NBS-LRR, EDS1, NDR1, NPR, RAR1, TFs and PR ) in V. vinifera. Next, the co-expression between the identified defensive genes was performed that tells us about the sequence of events that occurs during a signaling cascade in response to PM and DM-infection. Further, we characterized the identified genes on the basis of physico-chemical properties, chromosomal positions, gene and protein structure analysis and functional annotation. Conclusion: The powdery and downy mildew responsive defensive genes identified in this study can be used to develop new and improved PM and DM-resistant grape varieties in future.
Factors Affecting the Implementation of Continuous Quality Improvement in Health Facilities of Southern Nation and Nationalities People Region (SNNPR)
Authors: Yeshanew Addisu Gize , Wendwessen Nebiyou , Teklemariam Dereje 
Published: September 2019

Abstract
Continuous Quality improvement has become an important aspect of healthcare organizations. The objective of this study was to identify contributing factors for implementing continuous quality improvement projects in health facilities for the better delivery of health care service in the case of south nation and nationalities people, Ethiopia. Cross-sectional study was conducted from April to May 2018 in SNNPR of two district woredas health facilities. Descriptive, bivariate and multivariate analysis used, during the analysis, 0.05 p-value and 95% confidence interval (CI) was used to judge the significance of the associations. So that p-value less than 0.05 were taken as significant association. The finding of this study showed that, a total of 144 health professionals were participated, giving a response rate of 100%. Majority of the respondents (75%) were male and 35% said that their respective health facility implemented continuous quality improvement project. More than halve of (51.4%) the leaders were not receptive for new ideas; moreover, majority of leader were not encouraging learning (62.5%) and not engaged in quality improvement project implementation process (66%). Variables like leaders receptive to new ideas, leaders share information/ data about health facility service delivery status, health facility has a quality improvement project plan, staff know using indicators to tell progress about service delivery, health facility assess client satisfaction level, were the independent predictors of continuous quality improvement(CQI) project implementation.
Evaluation and comparison of six GRACE models to stratify undifferentiated chest pain at the emergency department
Authors: Zheng Wen , Wang Guangmei , Ma Jingjing , Wu Shuo , Zhang He , Zheng Jiaqi , Xu Feng , Wang Jiali , Chen Yuguo 
Published: September 2019

Abstract
Background

The Global Registry of Acute Coronary Events (GRACE) score is recommended for stratifying chest pain. However, there are six formulas used to calculate the GRACE score for different outcomes of acute coronary syndrome (ACS), including death (Dth) or composite of death and myocardial infarction (MI), during in hospital (IH), in 6 months after discharge (OH6m) or from admission to 6 months later (IH6m). The more appropriate one for stratification of undifferentiated chest pain remains unclear. We aimed to provide firstly comprehensive evaluation and comparison of six GRACE models to predict 30-day major adverse cardiac events (MACE) in acute chest pain at the emergency department (ED).

Methods

Patients with acute chest pain were consecutively recruited from August 24, 2015 to September 30, 2017 in EDs of two public hospitals in China. The primary outcome was MACE within 30 days, including death, acute myocardial infarction (MI), emergency revascularization, cardiac arrest and cardiogenic shock. GRACE scores were calculated retrospectively using the prospectively obtained data. Correlation, calibration, discrimination and reclassification of six GRACE models were evaluated.

Results

A total of 2886 patients were analyzed, with 590 (20.4%) patients getting outcomes. The GRACE (IH6mDthMI), GRACE (IHDthMI), GRACE (IHDth), GRACE (IH6mDth), GRACE (OH6mDth) and GRACE (OH6mDthMI) showed positive linear correlation with actual MACE rates (r≥0.568, p<0.001), first two of which exerted very strong relationships (r>0.9). All these models had good calibration (Hosmer-Lemeshow goodness-of-fit test, p≥0.073) except GRACE (IHDthMI) (p<0.001). The corresponding c-statistics were 0.82(0.81,0.83), 0.83(0.81,0.84), 0.75(0.73,0.76), 0.73(0.72,0.75), 0.72(0.70,0.73) and 0.70(0.68,0.71). Improvement in AUC, NRI and IDI (p<0.001) represented that GRACE (IH6mDthMI) and GRACE (IHDthMI) were superior to other four models in discrimination and reclassification.

Conclusions

The GRACE (IH6mDthMI) and GRACE (IHDthMI) outperformed other GRACE models in discriminating high or low-risk of 30-day MACE in patients with chest pain. The reasonable application of appropriate GRACE models should be recommended on stratification of undifferentiated chest pain presenting to the ED.
Upregulation of pERK and c-JUN by γ-tocotrienol and not α-tocopherol are essential to the differential effect on apoptosis in prostate cancer cells.
Authors: Moore Christine , Palau Victoria , Mahboob Rashid , Lightner Janet , Stone William , Krishnan Koyamangalath 
Published: September 2019

Abstract
Background: α-tocopherol (AT) and γ-tocotrienol (GT3) are vitamin E isoforms considered to have potential chemopreventive properties. AT has been widely studied in vitro and in clinical trials with mixed results. The latest clinical study (SELECT trial) tested AT in prostate cancer patients, determined that AT provided no benefit, and could promote cancer. Conversely, GT3 has shown antineoplastic properties in several in vitro studies, with no clinical studies published to date. GT3 causes apoptosis via upregulation of the JNK pathway; however, inhibition results in a partial block of cell death. We compared side by side the mechanistic differences in these cells in response to AT and GT3.
Methods: The effects of GT3 and AT were studied on androgen sensitive LNCaP and androgen independent PC-3 prostate cancer cells. Their cytotoxic effects were analyzed via MTT and confirmed by metabolic assays. Cellular pathways were studied by immunoblot. Quantitative analysis and the determination of relationships between cell signaling events were analyzed for both agents tested. Non-cancerous prostate RWPE-1 cells were also included as a control.
Results: The RAF/RAS/ERK pathway was significantly activated by GT3 in LNCaP and PC-3 cells but not by AT. This activation is essential for the apoptotic affect by GT3 as demonstrated the complete inhibition of apoptosis by MEK1 inhibitor U0126. Phospho-c-Jun was upregulated by GT3 but not AT. No changes were observed on AKT for either agent, and no release of cytochrome c into the cytoplasm was detected. Caspases 9 and 3 were efficiently activated by GT3 on both cell lines irrespective of androgen sensitivity, but not in cells dosed with AT. Cell viability of non-cancerous RWPE-1 cells was affected neither by GT3 nor AT.

Conclusions:
c-JUN is a recognized master regulator of apoptosis as shown previously in prostate cancer. However, the mechanism of action of GT3 in these cells also include a significant activation of ERK which is essential for the apoptotic effect of GT3. The activation of both, ERK and c-JUN, is required for apoptosis and may suggest a relevant step in ensuring circumvention of mechanisms of resistance related to the constitutive activation of MEK1.
Mental health help-seeking barriers, facilitators and interventions. A systematic review
Authors: Aguirre Antonia , Silva Ignacio , Billings Jo , Jimenez Magdalena , Rowe Sarah 
Published: September 2019

Abstract
Background: Increasing rates of mental health problems among adolescents are alarming. Teens who are most in need of mental health attention are reluctant to seek help. A better understanding of the help-seeking in this population is crucial to overcome this gap. Methods: Five databases were searched to identify the principal barriers, facilitators and interventions targeting help-seeking for common mental health problems in adolescents aged 10-19 years. The search was performed in June 2018 and updated in March 2019. Two independent screening processes were made using the eligibility criteria. Quality assessment of each study was performed and findings summarised using a narrative synthesis. Results: 90 studies meet the inclusion criteria for this review for barrier and facilitators (n=54) and interventions (n=36). Stigma and negative beliefs towards mental health services and professionals were the most cited barriers. Facilitators included previous positive experience with health services and mental health literacy. Most interventions were based on psychoeducation, which focused on general mental health knowledge, suicide and self-harm, stigma and depression. Other types of interventions included the use of multimedia and online tools, peer training and outreach initiatives. Overall, the quality of studies was low to medium and there was no general agreement regarding help-seeking definition and measurements. Conclusion: Most of interventions took place in an educational setting however, it is important to consider adolescents outside the educational system. Encouraging help-seeking should come with the increased availability of mental health support for all adolescents in need, but this is still a major challenge for Child and Adolescent Mental Health Services. There is also a need to develop shared definitions, theoretical frameworks and higher methodological standards in research regarding help-seeking behaviours in adolescents. This will allow more consistency and generalisability of findings, improving the development of help-seeking interventions and ensuring timely access to mental health treatments.
Opposite roles of BAP1 in overall survival of uveal melanoma and cutaneous melanoma
Authors: Liu-Smith Feng 
Published: September 2019

Abstract
Background: BAP1 germline mutations predispose individuals to a number of cancer types including uveal melanoma (UM) and cutaneous melanoma (CM) which are distinctively different in the oncogenic pathways. BAP1 loss was common in UM and was associated with a worse prognosis. BAP1 loss was rare in CM and the outcome was unclear. Methods: This study used TCGA UM and CM databases for survival analysis for patients with different BAP1 status and mRNA expression levels. Cox regression model was used for adjusting to known prognosis factors. Results: BAP1- (loss or low expression) predicted a poor overall survival in UM (Cox HR = 0.062, logrank p =0.007) but a contrasting better overall survival in CM (HR = 1.69, p =0.009). Multi-covariate Cox regression analysis indicated BAP1 was a significant predictor for overall survival after adjusting for age of diagnosis, presence of ulceration, Breslow depth and CM stages in patients older than 50 years but not in younger patients. Co-expression analysis revealed no shared genes in BAP1 altered UM and CM tumors, further supporting a completely distinctive role of BAP1 in CM and UM. Conclusions: low BAP1 mRNA was significantly associated with a better overall survival in CM patients, in sharp contrast to its tumor suppressor role in UM where low or loss of BAP1 indicated a worse overall survival. Function of BAP1 may be dependent on cellular context.