bluuebunny/crossref_metadata_2025_split huggingface dataset:
Maximum Standardized Uptake Value (SUVmax) of Primary Tumor Predicts Occult Neck Metastasis in Oral Cancer
Authors: Morand Grégoire B. , Vital Domenic G. , Kudura Ken , Werner Jonas , Stoeckli Sandro. J. , Huber Gerhard F. , Huellner Martin W. 
Published: August 2018

AbstractThe aim of this study was to investigate the predictability of occult lymph node metastasis using maximum standardized uptake value (SUVmax) in the primary tumor on pre-treatment 18-fluorodeoxyglucose positron emission tomography FDG-PET in oral squamous cell carcinoma (OSCC) patients who were clinically node negative (cN0) before surgery. A retrospective analysis of all patients treated at the University Hospital Zurich from 2007 to 2016 for OSCC with available pre-therapeutic FDG-PET was performed. We assessed the correlation of SUVmax of the primary tumors with the presence of occult nodal disease in the neck dissection specimen (pN+). The study included a total of 71 patients. In the nodal negative group (cN0/pN0), the median SUVmax of primary tumors was 9.0 (interquartile range (IQR) 7.4–13.9), while it was 11.4 (IQR 9.9–15.7) in the occult metastatic group (cN0/pN+). The difference was statistically significant (independent samples median test, P = 0.037). In a multivariable model, the only independent predictor of occult metastatic disease for cN0 patients was a SUVmax ≥ 9.5 (P = 0.028). Further, primary tumors with SUVmax ≥ 9.5 had a significantly higher risk of local recurrence (Log rank test, P = 0.020). In conclusion, we showed that higher SUVmax (≥9.5) of the primary tumor is associated with higher occurrence of occult metastatic nodal disease and worse local survival. High SUVmax of the primary tumor may encourage clinicians towards more aggressive treatment.
Y1 receptor deficiency in β-cells leads to increased adiposity and impaired glucose metabolism
Authors: Loh Kim , Shi Yan-Chuan , Bensellam Mohammed , Lee Kailun , Laybutt D. Ross , Herzog Herbert 
Published: August 2018

AbstractInsulin secretion from pancreatic β-cells is critical for maintaining glucose homeostasis and deregulation of circulating insulin levels is associated with the development of metabolic diseases. While many factors have been implicated in the stimulation of insulin secretion, the mechanisms that subsequently reduce insulin secretion remain largely unexplored. Here we demonstrate that mice with β-cell specific ablation of the Y1 receptor exhibit significantly upregulated serum insulin levels associated with increased body weight and adiposity. Interestingly, when challenged with a high fat diet these β-cell specific Y1-deficient mice also develop hyperglycaemia and impaired glucose tolerance. This is most likely due to enhanced hepatic lipid synthesis, resulting in an increase of lipid accumulation in the liver. Together, our study demonstrates that Y1 receptor signaling negatively regulates insulin release, and pharmacological inhibition of Y1 receptor signalling for the treatment of non-insulin dependent diabetes should be taken into careful consideration.
Expression, purification, and characterization of biologically active full-length Mason-Pfizer monkey virus (MPMV) Pr78Gag
Authors: Pitchai Fathima Nuzra Nagoor , Ali Lizna , Pillai Vineeta Narayana , Chameettachal Akhil , Ashraf Syed Salman , Mustafa Farah , Marquet Roland , Rizvi Tahir Aziz 
Published: August 2018

AbstractMPMV precursor polypeptide Pr78Gag orchestrates assembly and packaging of genomic RNA (gRNA) into virus particles. Therefore, we have expressed recombinant full-length Pr78Gag either with or without His6-tag in bacterial as well as eukaryotic cultures and purified the recombinant protein from soluble fractions of the bacterial cultures. The recombinant Pr78Gag protein has the intrinsic ability to assemble in vitro to form virus like particles (VLPs). Consistent with this observation, the recombinant protein could form VLPs in both prokaryotes and eukaryotes. VLPs formed in eukaryotic cells by recombinant Pr78Gag with or without His6-tag can encapsidate MPMV transfer vector RNA, suggesting that the inclusion of the His6-tag to the full-length Pr78Gag did not interfere with its expression or biological function. This study demonstrates the expression and purification of a biologically active, recombinant Pr78Gag, which should pave the way to study RNA-protein interactions involved in the MPMV gRNA packaging process.
Particle slip velocity influences inertial focusing of particles in curved microchannels
Authors: Deshpande Saurabh , Tallapragada Phanindra 
Published: August 2018

AbstractSize based separation and identification of particles in microfluidics through purely hydrodynamic means has gained significant interest due to a number of possible biomedical applications. Curved micro-channels, particularly spiral micro-channels with rectangular cross-section and the dynamics of particles in such channels have been extensively researched to achieve size based separation of particles. In this paper we present evidence that sheds new light on the dynamics of particles in such curved channels; that a unique particle slip velocity is associated with the focusing positions in the cross sections, which leads to a balance of forces. Our experiments therefore imply that the forces acting on the particle lead to convergence to an attractor in both the physical space (the cross section of the channel) and the slip velocity space.
An Integrated Local Classification Model of Predicting Drug-Drug Interactions via Dempster-Shafer Theory of Evidence
Authors: Shi Jian-Yu , Shang Xue-Qun , Gao Ke , Zhang Shao-Wu , Yiu Siu-Ming 
Published: August 2018

AbstractDrug-drug interactions (DDIs) may trigger adverse drug reactions, which endanger the patients. DDI identification before making clinical medications is critical but bears a high cost in clinics. Computational approaches, including global model-based and local model based, are able to screen DDI candidates among a large number of drug pairs by utilizing preliminary characteristics of drugs (e.g. drug chemical structure). However, global model-based approaches are usually slow and don’t consider the topological structure of DDI network, while local model-based approaches have the degree-induced bias that a new drug tends to link to the drug having many DDI. All of them lack an effective ensemble method to combine results from multiple predictors. To address the first two issues, we propose a local classification-based model (LCM), which considers the topology of DDI network and has the relaxation of the degree-induced bias. Furthermore, we design a novel supervised fusion rule based on the Dempster-Shafer theory of evidence (LCM-DS), which aggregates the results from multiple LCMs. To make the final prediction, LCM-DS integrates three aspects from multiple classifiers, including the posterior probabilities output by individual classifiers, the proximity between their instance decision profiles and their reference profiles, as well as the quality of their reference profiles. Last, the substantial comparison with three state-of-the-art approaches demonstrates the effectiveness of our LCM, and the comparison with both individual LCM implementations and classical fusion algorithms exhibits the superiority of our LCM-DS.
Thermal and impact histories of 25143 Itokawa recorded in Hayabusa particles
Authors: Terada K. , Sano Y. , Takahata N. , Ishida A. , Tsuchiyama A. , Nakamura T. , Noguchi T. , Karouji Y. , Uesugi M. , Yada T. , Nakabayashi M. , Fukuda K. , Nagahara H. 
Published: August 2018

AbstractUnderstanding the origin and evolution of near-Earth asteroids (NEAs) is an issue of scientific interest and practical importance because NEAs are potentially hazardous to the Earth. However, when and how NEAs formed and their evolutionary history remain enigmas. Here, we report the U-Pb systematics of Itokawa particles for the first time. Ion microprobe analyses of seven phosphate grains from a single particle provide an isochron age of 4.64 ± 0.18 billion years (1σ). This ancient phosphate age is thought to represent the thermal metamorphism of Itokawa’s parent body, which is identical to that of typical LL chondrites. In addition, the incorporation of other particles suggests that a significant shock event might have occurred 1.51 ± 0.85 billion years ago (1σ), which is significantly different from the shock ages of 4.2 billion years of the majority of shocked LL chondrites and similar to that of the Chelyabinsk meteorite. Combining these data with recent Ar-Ar studies on particles from a different landing site, we conclude that a globally intense impact, possibly a catastrophic event, occurred ca. 1.4 Ga ago. This conclusion enables us to establish constraints on the timescale of asteroid disruption frequency, the validity of the crater chronology and the mean lifetime of small NEAs.
Direct compression of 170-fs 50-cycle pulses down to 1.5 cycles with 70% transmission
Authors: Jeong Young-Gyun , Piccoli Riccardo , Ferachou Denis , Cardin Vincent , Chini Michael , Hädrich Steffen , Limpert Jens , Morandotti Roberto , Légaré François , Schmidt Bruno E. , Razzari Luca 
Published: August 2018

AbstractWe present a straightforward route for extreme pulse compression, which relies on moderately driving self-phase modulation (SPM) over an extended propagation distance. This avoids that other detrimental nonlinear mechanisms take over and deteriorate the SPM process. The long propagation is obtained by means of a hollow-core fiber (HCF), up to 6 m in length. This concept is potentially scalable to TW pulse peak powers at kW average power level. As a proof of concept, we demonstrate 33-fold pulse compression of a 1 mJ, 6 kHz, 170 fs Yb laser down to 5.1 fs (1.5 cycles at 1030 nm), by employing a single HCF and subsequent chirped mirrors with an overall transmission of 70%.
Course and characteristics of work disability 3 years before and after lumbar spine decompression surgery– a national population-based study
Authors: Dorner Thomas E. , Helgesson Magnus , Nilsson Kerstin , Pazarlis Konstantinos A. , Ropponen Annina , Svedberg Pia , Mittendorfer-Rutz Ellenor 
Published: August 2018

AbstractDespite decompression surgery being a widespread intervention for patients with dorsopathies (i.e. back pain) affecting the lumbar spine, the scientific knowledge on patterns and characteristics of work disability before and after the surgery is limited. Sickness absence (SA) and disability pension (DP) were examined three years before and after surgery in 8558 patients aged 25–60 years who underwent lumbar spine decompression surgery in Sweden. They were compared to individuals with diagnosed dorsopathies but no surgery and individuals from the general population as matched comparison groups. According to Group Based Trajectory models, in patients with decompression surgery, 39% had low levels of SA/DP during the entire study period and 15% started with low levels of SA/DP, which increased in the year before, and declined to almost zero in the second year after surgery. Three trajectory groups (12%, 17%, and 18%) started at different levels of SA/DP, which increased in the years before, and declined in the third year after surgery. The trajectory groups in the comparison groups showed lower levels of work disability. Sex, education, and the use of antidepressants and analgesics the year before surgery played an important role to explain the variance of trajectory groups in patients with surgery.
Intestinal regulation of suppression of tumorigenicity 14 (ST14) and serine peptidase inhibitor, Kunitz type -1 (SPINT1) by transcription factor CDX2
Authors: Danielsen E. Thomas , Olsen Anders Krüger , Coskun Mehmet , Nonboe Annika W. , Larsen Sylvester , Dahlgaard Katja , Bennett Eric Paul , Mitchelmore Cathy , Vogel Lotte Katrine , Troelsen Jesper Thorvald 
Published: August 2018

AbstractThe type II membrane-anchored serine protease, matriptase, encoded by suppression of tumorgenicity-14 (ST14) regulates the integrity of the intestinal epithelial barrier in concert with its inhibitor, HAI-1 encoded by serine peptidase inhibitor, Kunitz type -1 (SPINT1). The balance of the protease/inhibitor gene expression ratio is vital in preventing the oncogenic potential of matriptase. The intestinal cell lineage is regulated by a transcriptional regulatory network where the tumor suppressor, Caudal homeobox 2 (CDX2) is considered to be an intestinal master transcription factor. In this study, we show that CDX2 has a dual function in regulating both ST14 and SPINT1, gene expression in intestinal cells. We find that CDX2 is not required for the basal ST14 and SPINT1 gene expression; however changes in CDX2 expression affects the ST14/SPINT1 mRNA ratio. Exploring CDX2 ChIP-seq data from intestinal cell lines, we identified genomic CDX2-enriched enhancer elements for both ST14 and SPINT1, which regulate their corresponding gene promoter activity. We show that CDX2 displays both repressive and enhancing regulatory abilities in a cell specific manner. Together, these data reveal new insight into transcriptional mechanisms controlling the intestinal matriptase/inhibitor balance.
Next-generation amplicon sequencing identifies genetically diverse human astroviruses, including recombinant strains, in environmental waters
Authors: Hata Akihiko , Kitajima Masaaki , Haramoto Eiji , Lee Suntae , Ihara Masaru , Gerba Charles P. , Tanaka Hiroaki 
Published: August 2018

AbstractHuman astroviruses are associated with gastroenteritis and known to contaminate water environments. Three different genetic clades of astroviruses are known to infect humans and each clade consists of diverse strains. This study aimed to determine the occurrence and genetic diversity of astrovirus strains in water samples in different geographical locations, i.e., influent and effluent wastewater samples (n = 24 each) in Arizona, U.S., and groundwater (n = 37) and river water (n = 14) samples collected in the Kathmandu Valley, Nepal, using next-generation amplicon sequencing. Astrovirus strains including rare types (types 6 and 7 classical human astroviruses), emerging type (type 5 VA-astroviruses), and putative recombinants were identified. Feline astrovirus strains were collaterally identified and recombination between human and feline astroviruses was suggested. Classical- and VA-astroviruses seemed to be prevalent during cooler months, while MLB-astroviruses were identified only during warmer months. This study demonstrated the effectiveness of next-generation amplicon sequencing for identification and characterization of genetically diverse astrovirus strains in environmental water.
First evidence of the presence and activity of archaeal C3 group members in an Atlantic intertidal mudflat
Authors: Lavergne Céline , Hugoni Mylène , Dupuy Christine , Agogué Hélène 
Published: August 2018

AbstractThe phylogenetic assignment of archaeal communities is constantly evolving, and the recent discovery of new phyla that grouped into superphyla has provided novel insights into archaeal ecology and evolution in ecosystems. In intertidal sediments, archaea are known to be involved in key functional processes such as organic matter turnover, but the ecological relevance of the rarest archaeal groups is poorly investigated, due partly to the lack of cultivated members. The high resolution of microbial diversity provided by high-throughput sequencing technologies now allows the rare biosphere to be described. In this work, we focused on the archaeal C3 group, showing that this phylum is not only present (at the DNA level) independently of sediment depth but also active (at the RNA level) in specific sediment niches depending on vertical physicochemical gradients. Moreover, we highlight the ambiguous phylogenetic affiliation of this group, indicating the need of further research to get new insights into the role of the C3 group.
Reversine inhibits Colon Carcinoma Cell Migration by Targeting JNK1
Authors: Jemaà Mohamed , Abassi Yasmin , Kifagi Chamseddine , Fezai Myriam , Daams Renée , Lang Florian , Massoumi Ramin 
Published: August 2018

AbstractColorectal cancer is one of the most commonly diagnosed cancers and the third most common cause of cancer-related death. Metastasis is the leading reason for the resultant mortality of these patients. Accordingly, development and characterization of novel anti-cancer drugs limiting colorectal tumor cell dissemination and metastasis are needed. In this study, we found that the small molecule Reversine reduces the migration potential of human colon carcinoma cells in vitro. A coupled kinase assay with bio-informatics approach identified the c-Jun N-terminal kinase (JNK) cascade as the main pathway inhibited by Reversine. Knockdown experiments and pharmacological inhibition identified JNK1 but not JNK2, as a downstream effector target in cancer cell migration. Xenograft experiments confirm the effect of JNK inhibition in the metastatic potential of colon cancer cells. These results highlight the impact of individual JNK isoforms in cancer cell metastasis and propose Reversine as a novel anti-cancer molecule for treatment of colon cancer patients.
Transition processes of a static multilevel atom in the cosmic string spacetime with a conducting plane boundary
Authors: Cai Huabing , Ren Zhongzhou 
Published: August 2018

AbstractWe investigate the transition processes of a static multilevel atom in interaction with a fluctuating vacuum quantum electromagnetic field in the cosmic string spacetime in the presence of an infinite, perfectly conducting plane. Using the formalism proposed by DDC, we find that the presence of the boundary modifies both vacuum fluctuations and radiation reaction contributions to the atomic spontaneous emission rate. Our results indicate that the total decay rate and the boundary-induced contribution both depend upon the atom-string distance, the atom-plate separation, the extent of the polar angle deficit induced by the string, and the atomic polarization direction. By adjusting these parameters, the atomic decay rate can be either enhanced or weakened significantly by the boundary. Moreover, the presence of the boundary can distinguish certain polarization directions that bring about the same decay rate in the case of a free cosmic string spacetime. Theoretically, our work suggests a more flexible means to adjust and control the radiative processes of atoms.
Estimation of elevated intracranial pressure in infants with hydroce-phalus by using transcranial Doppler velocimetry with fontanel compression
Authors: Yoshizuka Teiko , Kinoshita Masahiro , Iwata Sachiko , Tsuda Kennosuke , Kato Takenori , Saikusa Mamoru , Shindou Ryota , Hara Naoko , Harada Eimei , Takashima Sachio , Takeshige Nobuyuki , Saitoh Shinji , Yamashita Yushiro , Iwata Osuke 
Published: August 2018

AbstractFor infants with acute progressive hydrocephalus, invasive drainage of cerebrospinal fluid (CSF) is performed until a ventriculo-peritoneal shunt can be inserted. Surrogate markers of intracranial pressure (ICP) may help optimise the timing of invasive procedures. To assess whether RI with/without fontanel compression helps distinguish between infants with normal (<5 cmH2O), mild (5–11 cmH2O), and moderate (>11 cmH2O) ICP elevation, 74 ICP measures before/after CSF removal and 148 related Doppler measures of the middle cerebral artery were assessed. Higher RI was associated with fontanel compression, elevated ICP, and their interaction (all p < 0.001). Without compression, differences in RI were observed between normal and moderate (p < 0.001) and between mild and moderate ICP elevation (p = 0.033). With compression, differences in RI were observed for all pairwise comparisons among normal, mild, and moderate ICP elevation (all p < 0.001). Without compression, areas under the receiver-operating characteristic curve for prediction of mild and moderate ICP elevation were 0.664 (95% confidence interval (CI), 0.538–0.791; p = 0.020) and 0.727 (95% CI, 0.582–0.872; p = 0.004), respectively, which improved to 0.806 (95% CI, 0.703–0.910; p < 0.001) and 0.814 (95% CI, 0.707–0.921; p < 0.001), respectively, with compression. RI with fontanel compression provides improved discrimination of infants with absent, mild, and moderate ICP elevation.
A nano-silicate material with exceptional capacity for CO2 capture and storage at room temperature
Authors: Cavalcanti Leide P. , Kalantzopoulos Georgios N. , Eckert Juergen , Knudsen Kenneth D. , Fossum Jon Otto 
Published: August 2018

AbstractIn order to mitigate climate change driven by the observed high levels of carbon dioxide (CO2) in the atmosphere, many micro and nano-porous materials are being investigated for CO2 selectivity, capture and storage (CCS) purposes, including zeolites, metal organic frameworks (MOFs), functionalized polymers, activated carbons and nano-silicate clay minerals. Key properties include availability, non-toxicity, low cost, stability, energy of adsorption/desorption, sorbent regeneration, sorption kinetics and CO2 storage capacity. Here, we address the crucial point of the volumetric capture and storage capacity for CO2 in a low cost material which is natural, non-toxic, and stable. We show that the nano-silicate Nickel Fluorohectorite is able to capture 0.79 metric tons of CO2 per m3 of host material - one of the highest capacities ever achieved - and we compare volumetric and gravimetric capacity of the best CO2 sorbent materials reported to date. Our results suggest that the high capture capacity of this fluorohectorite clay is strongly coupled to the type and valence of the interlayer cation (here Ni2+) and the high charge density, which is almost twice that of montmorillonite, resulting in the highest reported CO2 uptake among clay minerals.
Serotonin depletion causes valproate-responsive manic-like condition and increased hippocampal neuroplasticity that are reversed by stress
Authors: Maddaloni Giacomo , Migliarini Sara , Napolitano Francesco , Giorgi Andrea , Nazzi Serena , Biasci Daniele , De Felice Alessia , Gritti Marta , Cavaccini Anna , Galbusera Alberto , Franceschi Sara , Lessi Francesca , Ferla Marco La , Aretini Paolo , Mazzanti Chiara Maria , Tonini Raffaella , Gozzi Alessandro , Usiello Alessandro , Pasqualetti Massimo 
Published: August 2018

AbstractAbnormal hippocampal neural plasticity has been implicated in behavioural abnormalities and complex neuropsychiatric conditions, including bipolar disorder (BD). However, the determinants of this neural alteration remain unknown. This work tests the hypothesis that the neurotransmitter serotonin (5-HT) is a key determinant of hippocampal neuroplasticity, and its absence leads to maladaptive behaviour relevant for BD. Depletion of brain 5-HT in Tph2 mutant mice resulted in reduced behavioural despair, reduced anxiety, marked aggression and lower habituation in novel environments, reminiscent of bipolar-associated manic behaviour. Treatment with valproate produced a substantial improvement of the mania-like behavioural phenotypes displayed by Tph2 mutants. Brain-wide fMRI mapping in mutants revealed functional hippocampal hyperactivity in which we also observed dramatically increased neuroplasticity. Importantly, remarkable correspondence between the transcriptomic profile of the Tph2 mutant hippocampus and neurons from bipolar disorder patients was observed. Chronic stress reversed the emotional phenotype and the hippocampal transcriptional landscape of Tph2 mutants. These changes were associated with inappropriate activation of transcriptional adaptive response to stress as assessed by gene set enrichment analyses in the hippocampus of Tph2 mutant mice. These findings delineate 5-HT as a critical determinant in BD associated maladaptive emotional responses and aberrant hippocampal neuroplasticity, and support the use of Tph2−/− mice as a new research tool for mechanistic and therapeutic research in bipolar disorder.