bluuebunny/crossref_metadata_2025_split huggingface dataset:
Establishing methods to monitor H5N1 influenza virus in dairy cattle milk
Authors: Stachler Elyse , Gnirke Andreas , McMahon Kyle , Gomez Michael , Stenson Liam , Guevara-Reyes Charelisse , Knoll Hannah , Hill Toni , Hill Sellers , Messer Katelyn S. , Arizti-Sanz Jon , Albeez Fatinah , Curtis Elizabeth , Samani Pedram , Wewior Natalia , O’Connor David H. , Vuyk William , Khoury Sophia , Schnizlein Matthew K. , Rockey Nicole C. , Broemmel Zachariah , Mina Michael , Madoff Lawrence C. , Wohl Shirlee , O’Connor Lorraine , Brown Catherine M. , Ozonoff Al , Park Daniel J. , MacInnis Bronwyn L. , Sabeti Pardis C. 
Published: December 2024

AbstractHighly Pathogenic Avian Influenza strain H5N1 has caused a multi-state outbreak among US dairy cattle, spreading across 15 states and infecting hundreds of herds since its onset. We rapidly developed and optimized PCR-based detection assays and sequencing protocols to support H5N1 molecular surveillance. Using 214 retail milk from 20 states for methods development, we found that H5N1 concentrations by digital PCR strongly correlated with qPCR cycle threshold (Ct) values, with dPCR exhibiting greater sensitivity. We also found that metagenomic sequencing after hybrid selection was best for higher concentration samples while amplicon sequencing performs best for lower concentrations. By establishing these methods, we were able to support the creation of a statewide surveillance program to test bulk milk samples monthly from all cattle dairy farms within Massachusetts, which remain negative to date. The methods, workflow, and recommendations described here provide a framework for others aiming to conduct H5N1 surveillance efforts.
A Decade of Radiological Examinations in the Emergency Department: A Monocentric Retrospective Observational Study
Authors: Goumballa Ndiaw , de Oliveira Fabien , Coisy Fabien , Goupil Jean , Frandon Julien , Longueville Florence , Daladouire Catherine , Grandpierre Romain Genre , Beregi Jean Paul 
Published: December 2024

AbstractPurposeTo analyze the evolution of various radiological examinations requested for patients admitted to the emergency department (ED) of a University Hospital.Materials and MethodsWe analyzed data on MRI, CT scan, Ultrasound (US), and X-ray activities, along with ED patient admissions over a ten-year period from 2014 to 2023. The patient data for each modality, categorized by age and sex, were extracted from the Radiological Information System database.ResultsThe number of patients undergoing radiological examinations increased from 35,532 in 2014 to 44,592 in 2023, marking a 25.9% increase, while the number of ED admissions rose from 71,776 to 103,456, a 44.1% increase. The ratio of radiological examinations to ED admissions decreased from 49.5% in 2014 to 43.1% in 2023. Over the study period, the number of patients receiving MRI and CT scans increased by 80.4% and 105.8%, respectively. The number of patients undergoing ultrasound remained relatively stable, with 2,616 in 2014 and 2,432 in 2023. In contrast, the number of X-ray patients decreased by 33.1% from 22,236 in 2014 to 14,847 in 2020 but rebounded to 20,492 in 2023. Male patients more frequently underwent CT (51.7%) and X-ray examinations (53.3%).ConclusionThis study highlights a significant increase in radiological activity within the emergency department, especially in CT scans and MRI usage over the ten-year period while ultrasound examinations stayed flat, accompanied by a decline in the ratio of radiological examinations to ED admissions.
Crackling Cloud: an event-driven, cloud-based CRISPR-Cas9 guide RNA design tool
Authors: Bradford Jacob , Joy Divya , Winsen Mattias , Meurant Nicholas , Wilkins Mackenzie , Wilson Laurence , Bauer Denis , Perrin Dimitri 
Published: December 2024

AbstractGene editing has been revolutionised by the CRISPR-Cas9 technology. The versatility and ease-of-use of the technology far exceeds its predecessors, however, the selection of a high-quality guide RNA (gRNA) is critical to directing it to a target site. Selecting gRNA calls upon high-performance algorithms that evaluate nuclease activity at on-target and off-target sites. While there are a suite of programs available, many struggle to analyse the largest genomes, or their predictive accuracy is low. We have previously published a program, named Crackling that is amongst the fastest and most accurate tools available, however, it requires an end-user to have access to a traditional high-performance computing environment. Here, we present an adaptation of Crackling, named Crackling Cloud, that takes advantage of modern serverless cloud technologies that are widely available to anyone, and do not consume resources and incur costs when sitting idle, but can scale to use large volumes of resources when analyses require that. Crackling Cloud is provided as a templated solution using technologies of Amazon Web Services, and is available for free on GitHub under the terms of the BSD 3-clause licence:https://github.com/bmds-lab/Crackling-AWS
Pseudomonas aeruginosaperforms chemotaxis to serotonin, dopamine, epinephrine, and norepinephrine
Authors: Monteagudo-Cascales Elizabet , Lozano-Montoya Andrea , Krell Tino 
Published: December 2024

AbstractBacteria use chemotaxis to move to favorable ecological niches. For many pathogenic bacteria, chemotaxis is required for full virulence, particularly for the initiation of host colonization. There do not appear to be limits to the type of compounds that attract bacteria, and we are just beginning to understand how chemotaxis adapts them to their lifestyles. Quantitative capillary assays for chemotaxis show thatP. aeruginosais strongly attracted to serotonin, dopamine, epinephrine, and norepinephrine. Chemotaxis to these compounds is greatly decreased in a mutant lacking the TlpQ chemoreceptor, and complementation of this mutant with a plasmid harboring thetlpQgene restores wild-type-like chemotaxis. Microcalorimetric titrations of the TlpQ sensor domain with these four compounds indicate that they bind directly to TlpQ. All four compounds are hormones and neurotransmitters that control a variety of processes and are also important signal molecules involved in the virulence ofP. aeruginosa. They modulate motility, biofilm formation, the production of virulence factors and serve as siderophores that chelate iron. Therefore, chemotaxis to these four compounds is likely to alterP. aeruginosavirulence. Additionally, we believe that this is the first report of bacterial chemotaxis to serotonin and dopamine. This study provides an incentive for research to define the contribution of chemotaxis to these host signaling molecules to the virulence ofP. aeruginosa.
Prp16 enables efficient splicing of introns with diverse exonic consensus elements in the short-intron richCryptococcus neoformanstranscriptome
Authors: Negi Manendra Singh , Krishnan Vishnu Priya , Saraf Niharika , Vijayraghavan Usha 
Published: December 2024

AbstractThe DEAH box splicing helicase Prp16 in budding yeast governs spliceosomal remodeling from the branching conformation (C complex) to the exon ligation conformation (C* complex). In this study, we examined the genome-wide functions of Prp16 in the short intron-rich genome of the basidiomycete yeastCryptococcus neoformans. The presence of multiple introns per transcript with intronic features more similar to higher eukaryotes makes it a promising model to study spliceosomal splicing. Using a promoter-shutdown conditional Prp16 knockdown strain, we uncovered its genome-wide but substrate-specific roles inC. neoformanssplicing. The splicing functions of Prp16 are dependent on its helicase motif I and motif II that are conserved motifs for helicase activity. A small subset of introns spliced independent of Prp16 activity, were investigated to discover that exonic sequences at the 5’ splice site (5’SS) and 3’ splice site (3’SS) with stronger affinity for U5 loop 1 as a common feature in these introns. Furthermore, short (60-100nts) and ultra-short introns (<60nts) prevalent in theC. neoformanstranscriptome were more sensitive to Prp16 knockdown than longer introns, indicating Prp16 is required for the efficient splicing of short and ultra-short introns. We propose that stronger U5 snRNA-pre-mRNA interactions enable the efficient transition of the spliceosome from the first to the second catalytic confirmation in Prp16 knockdown, particularly for short introns and introns with suboptimal features. This study provides insights into the fine-tuning spliceosomal helicase functions with variations incis-element features.
Mycobacterium tuberculosisCrgA Forms a Dimeric Structure with Its Transmembrane Domain Sandwiched between Cytoplasmic and Periplasmic β-Sheets, Enabling Multiple Interactions with Other Divisome Proteins
Authors: Shin Yiseul , Prasad Ramesh , Das Nabanita , Taylor Joshua A. , Qin Huajun , Hu Wenhao , Hu Yan-Yan , Fu Riqiang , Zhang Rongfu , Zhou Huan-Xiang , Cross Timothy A. 
Published: December 2024

AbstractCrgA is a key transmembrane (TM) protein in the cell division process ofMycobacterium tuberculosis(Mtb), the pathogen responsible for tuberculosis. While many of theMtbdivisome proteins have been identified, their structures and interactions remain largely unknown. Previous studies of CrgA using oriented-sample solid-state NMR have defined the tilt and rotation of the TM helices, but the cytoplasmic and periplasmic domains and even the oligomeric state were uncharacterized. Here, combining oriented-sample and magic-angle spinning solid-state NMR spectra, we solved the full-length structure of CrgA. The structure features a dimer with a TM domain sandwiched between a cytoplasmic β-sheet and a periplasmic β-sheet. The β-sheets stabilize dimerization, which in turn increases CrgA’s ability to participate in multiple protein interactions. Within the membrane, CrgA binds FtsQ, CwsA, PbpA, FtsI, and MmPL3 via its TM helices; in the cytoplasm, Lys23 and Lys25 project outward from the β-sheet to interact with acidic residues of FtsQ and FtsZ. The structural determination of CrgA thus provides significant insights into its roles in recruiting other divisome proteins and stabilizing their complexes forMtbcell wall synthesis and polar growth.
Structural mechanisms of PIP2activation and SEA0400 inhibition in human cardiac sodium-calcium exchanger NCX1
Authors: Xue Jing , Zeng Weizhong , John Scott , Attiq Nicole , Ottolia Michela , Jiang Youxing 
Published: December 2024

AbstractNa+/Ca2+exchangers (NCXs) transport Ca2+across the plasma membrane in exchange for Na+and play a vital role in maintaining cellular Ca2+homeostasis. Our previous structural study of human cardiac NCX1 (HsNCX1) reveals the overall architecture of the eukaryotic exchanger and the formation of the inactivation assembly by the intracellular regulatory domain that underlies the cytosolic Na+-dependent inactivation and Ca2+activation of NCX1. Here we present the cryo-EM structures of HsNCX1 in complex with a physiological activator phosphatidylinositol 4,5-bisphosphate (PIP2), or pharmacological inhibitor SEA0400 that enhances the inactivation of the exchanger. We demonstrate that PIP2binding stimulates NCX1 activity by inducing a conformational change at the interface between the TM and cytosolic domains that destabilizes the inactivation assembly. In contrast, SEA0400 binding in the TM domain of NCX1 stabilizes the exchanger in an inward-facing conformation that facilitates the formation of the inactivation assembly, thereby promoting the Na+-dependent inactivation of NCX1. Thus, this study reveals the structural basis of PIP2activation and SEA0400 inhibition of NCX1 and provides some mechanistic understandings of cellular regulation and pharmacology of NCX family proteins.
Engaging leaders, proactive followers: engaging leadership and followers’ job crafting, performance and intrapreneurship
Authors: Get Wasim , Oprea Bogdan 
Published: December 2024

PurposeIn the current economy, organizations operate in an unpredictable and changing environment in which employees’ performance and proactivity can represent strategic advantages. However, it is not clear how managers can stimulate the performance and proactivity of subordinates. The purpose of the current study was to investigate the relationships between engaging leadership (an approach to managing people that is focused on fulfilling followers’ psychological needs for autonomy, competence, relatedness and meaningfulness) and subordinates’ job performance and intrapreneurship (organizational venture creation and the strategic renewal generated by employees within the company they work for). Based on the self-determination theory and job demands-resources model, we expected followers’ work engagement and job crafting (changes regarding job resources and demands that employees proactively make) to mediate the link between engaging leadership and followers’ performance and intrapreneurship.Design/methodology/approachA sample of 401 employees completed the study questionnaire. Employees evaluated their direct supervisors’ engaging leadership and reported on their own work engagement, job crafting, performance and intrapreneurship. The proposed relationships were tested using SEM.FindingsResults indicated that the relationships between engaging leadership and followers’ job performance and intrapreneurship were mediated by followers’ work engagement and two job crafting components (increasing structural resources and increasing challenging demands).Originality/valueThis study expands the range of outcomes of engaging leadership. In addition, it provides an explanatory mechanism for the relationship between engaging leadership and followers’ proactivity.
Supervision mechanism of integrated construction consultancy services in China: tripartite game analysis
Authors: Mao Xingjie , Deng Binchao , Zhao Xianbo , Lv Xindong 
Published: December 2024

PurposeMegaprojects contribute greatly to China’s socio-economic development. However, the diverse stakeholders of megaprojects tend to be prone to conflict and plunge the project into lower performance than expected. This study aims to investigate (1) the optimal supervision mechanism under direct supervision of the owner and (2) the optimal indirect supervision mechanism of the owner, with involvement of integrated construction consultancy (ICC) in supervision and considerations into the impact of collusion between the ICC and the general contractor on supervision efficiency.Design/methodology/approachThis study applies the principal–agent theory and game theory to design effective incentive and punishment mechanisms for direct and indirect supervision by the owner. Through the theoretical framework of game theory, it analyses the strategic interactions between the owner, the ICC and the general contractor, and evaluates the effectiveness of various supervision mechanisms based on Nash equilibrium theory.FindingsThe results showed adopting the direct supervision mechanism when the probability of the general contractor’s opportunistic behavior being detected was higher or the occurrence had a greater impact on the owner. However, the increase in supervision cost would reduce the motivation of owner’s direct supervision and prompt the owner to choose the indirect supervision mechanism. Additionally, the indirect supervision mechanism was more likely to inhibit the collusion between the ICC and the general contractor. Thus, increasing penalties for ICC would result in improved supervision of ICC, thereby improving the benefits to the owner.Originality/valueThis study examines the dynamic interaction between the owner, the general contractor and the ICC. The study establishes a game tree and game matrix based on principal–agent theory, and analyses direct and indirect supervision models to determine the Nash equilibrium and optimal supervision strategy that is in the best interest of the owner. The study advocates for improved management and supervision mechanisms and argues that tailored supervision mechanisms can significantly improve the performance of megaprojects, which is well validated in megaprojects in China.
Principals’ leadership styles and teachers’ organizational citizenship behaviour: a systematic review of research
Authors: Ahmed Eman I. 
Published: December 2024

PurposeThe purpose of this review was to expand the knowledge base regarding the relationship between principals’ leadership styles and teachers’ organizational citizenship behaviours drawing on a database of 87 studies. The study utilized four different data sources (WoS, Scopus, ERIC, and Google Scholar).Design/methodology/approachDescriptive and content analysis methods were used to highlight the key tends in this literature, including growth trajectory, authorship trends, citation impact, quantitative research techniques, the composition of leadership styles, and detailed mediators and moderators.FindingsThe results revealed that transformational leadership was the most frequently studied leadership style. The study synthesized how principal leadership directly and indirectly affects teachers’ OCBs. The findings were interpreted considering existing educational leadership and management (EDLM) literature.Originality/valueGiven the rapid growth of research on the linkage between principals’ leadership styles and teachers’ OCBs and the fact that the field has been missing a comprehensive review to lay out the relevant literature on this topic, it is important to systematically review and scrutinize the relationship between leadership styles and teachers’ OCB. Reviewing this literature will provide guidance for future researchers through identifying major methodological gaps and suggesting recommendations for future directions. This study is the first review on the relationship between principals’ leadership styles and teachers’ organizational citizenship behaviours (OCBs).
Psychological flexibility as a resource for preventing compulsive work and promoting well-being: a JD-R framework study
Authors: Russo Angela , Mansouri Mohammed , Santisi Giuseppe , Zammitti Andrea 
Published: December 2024

Purpose
In today’s high-demand work environments, characterised by an expectation for individuals to possess resources to manage workloads effectively, workaholism poses a significant threat to employee well-being. This study aims to investigate the impact of work overload and psychological flexibility on compulsive work behaviours and well-being.

Design/methodology/approach
This study applies the Job Demands-Resources model and uses structural equation modelling to analyse data collected from 305 adult workers aged 19–65. Psychological flexibility and work overload are examined as antecedents of compulsive work behaviour, with flourishing and life satisfaction as outcomes.

Findings
The results indicate that compulsive work behaviour mediates the relationship between work overload and psychological flexibility on well-being outcomes. Psychological flexibility was found to be a crucial resource in reducing workaholic tendencies, leading to improved flourishing and life satisfaction.

Research limitations/implications
The implications for human resources include practical strategies and targeted interventions to help individuals navigate organisational demands, prevent compulsive work behaviours and improve overall well-being.

Originality/value
This study offers new insights into the role of psychological flexibility as a personal resource in reducing compulsive work tendencies and enhancing both hedonic and eudaimonic well-being in high-demand work environments.
The antecedent, moderator and consequence of humor behaviors in entrepreneurial teams
Authors: Fu Rong , Kong Xiangyi , Gao Lifang , Zhao Lina 
Published: December 2024

PurposeBy integrating insights from cognitive style literature and person-environment fit theory, our study aims to explore which type of entrepreneurs are more inclined to initiate humor behaviors, the circumstances under which such behaviors are more likely to be employed within entrepreneurial teams and the specific benefits of humor behaviors for initiators.Design/methodology/approachThis paper analyzes humor behaviors in entrepreneurial teams through a three-wave time-lagged field survey of 227 entrepreneurs in China.FindingsWe find that entrepreneurs with higher creating style are more likely to exhibit humor, making them more popular within entrepreneurial teams. Additionally, the positive relationship is amplified in the presence of high levels of conflict.Originality/valuePrevious studies provide substantial evidence of the positive effects of humor behaviors on their recipients in the workplace; however, there has been limited focus on the antecedents of humor behaviors and the benefits for the humor initiators. Our study addresses the gaps by providing empirical evidence that creating style of entrepreneurs may promote humor behaviors, particularly in the context of conflicts within entrepreneurial teams, and such behaviors could elevate entrepreneurs’ social acceptance within teams.
Elementary econometric and strategic analysis of curling matches
Authors: Fry John , Austin Mark , Fanzon Silvio 
Published: December 2024

PurposeWe develop a Markov model of curling matches. This enables strategic and econometric analyses to be performed alongside computer simulation work.Design/methodology/approachWe develop a Markov model of curling matches, parametrised by the probability of winning an end and the probability distribution of scoring ends. In practical applications, these end-winning probabilities can be estimated econometrically and are shown to depend on which team holds the hammer as well as the offensive and defensive strengths of the respective teams. Using a maximum entropy argument, based on the idea of characteristic scoring patterns in curling, we predict that the points distribution of scoring ends should follow a constrained geometric distribution.FindingsWe provide analytical results detailing when it is optimal to blank the end in preference to scoring one point and losing possession of the hammer. Statistical and simulation analysis of international curling matches is also performed.Originality/valueOur work adds to the theory and application of sports analytics, especially Markov models, and to the econometric and strategic analysis of curling matches.