bluuebunny/crossref_metadata_2025_split huggingface dataset:
An opinion paper of the Cardiology Practice and Research Network of the American College of Clinical Pharmacy: Recommendations for training of cardiovascular pharmacy specialists in postgraduate year 2 residency programs
Authors: Ng Tien M. H. , DiDomenico Robert J. , Ripley Toni L. , Benge Cassandra D. , Buckley Leo F. , Campbell Kristen B. , Hale Genevieve M. , Macaulay Tracy E. , Nappi Jean M. , Pickworth Kerry K. , Short Marintha R. 
Published: June 2019

AbstractPharmacists in direct patient care settings are expanding their roles and responsibilities. These changes mandate a deeper and broader understanding of disease states, as well as influencing social, behavioral, and environmental factors. Existing guidelines and accreditation standards related to the training of graduate or cardiovascular pharmacist specialists (ie, Accreditation Council for Graduate Medical Education, American Society of Health‐System Pharmacists, American College of Clinical Pharmacy [ACCP]) provide some guidance on essential competencies. However, they stop short of providing recommendations for how to achieve all the objectives. The purpose of this paper is to build upon existing guidelines/standards, describing our recommendations for pharmacy residency training of a cardiovascular clinical specialist. The paper is broken down into the following sections: (1) Skills and Competencies (Building Clinical Skills, Application of Clinical Knowledge and Skills, Drug Information, Research and Scholarship, Teaching Skills, Interpersonal, Communication, and Presentation Skills), (2) Personal and Professional Growth (Growing Interpersonal Skills, Engaging with the Profession), (3) Program Design (Resident Selection, Preceptors and Mentoring, Expectations for Progress/Milestones, Program and Learning Experience Structure), and (4) Clinical and Therapeutic Content Expertise or Medical Knowledge. After each recommendation, specific details are provided to aid in conceptualizing how each can be achieved. Some recommendations are considered essential whereas others are designated as optional. This paper represents the opinion of the Cardiology Practice and Research Network of the American College of Clinical Pharmacy. It does not necessarily represent an official ACCP commentary, guideline, or statement of policy or position.
Diaphragm‐based optical fiber sensor for pulse wave monitoring and cardiovascular diseases diagnosis
Authors: Wang Jingyi , Liu Kewei , Sun Qizhen , Ni Xiaoling , Ai Fan , Wang Senmao , Yan Zhijun , Liu Deming 
Published: June 2019

AbstractArterial pulse wave has been considered as a vital sign in assessment of cardiovascular diseases. Noninvasive pulse sensor with compact structure, immunity to electro‐magnetic interference and high sensitivity is the research focus in recent years. While, optical fiber biosensor is a competitive option to meet these needs. Here, a diaphragm‐based optical fiber pulse sensor was proposed to achieve high‐precision radial pulse wave monitoring. A wearable device was developed, composed of a sports wristband and an aluminum diaphragm‐based optical fiber sensor tip of only 1 cm in diameter, which was highly sensitive to the weak acoustic signal. In particular, coherent phase detection was adopted to improve detection signal‐to‐noise ratio, so as to recover the high‐fidelity pulse waveforms. A clinical experiment was carried out to detect and morphological analyze the pulse waveforms of four subjects, the results of which preliminarily demonstrated the feasibility of pulse diagnosis method. The proposed pulse fiber sensor provides a comfortable way for pulse diagnosis, which is promising in early cardiovascular diseases indicating.
LncRNA CCAT1 promotes autophagy via regulating ATG7 by sponging miR‐181 in hepatocellular carcinoma
Authors: Guo Jianbo , Ma Yingbo , Peng Xueqiang , Jin Hongyuan , Liu Jingang 
Published: June 2019

AbstractBackgroundHepatocellular carcinoma (HCC) is a significant clinical challenge, and the mechanisms underlying HCC pathogenesis remain incompletely understood. Colon cancer associated transcript 1 (CCAT1), is one novel long noncoding RNA (lncRNA) which is upregulated in HCC. Autophagy is a vital process in HCC progression, and it is unknown whether CCAT1 regulates autophagy in HCC.Materials and methodsImmunofluorescence staining and transmission electron microscopy were used to analyze autophagy activity. Luciferase assay was performed to confirm miRNA‐181a‐5p (miR‐181a‐5p) bind CCAT1 and ATG7.ResultsCCAT1 levels were higher in tissue and cell lines of HCC. In function research, we found that CCAT1 facilitates HCC cell autophagy and cell proliferation. Our results show that, mechanistically, CCAT1 promotes autophagy through functioning as a sponge for miR‐181a‐5p, and then regulating ATG7 expression.ConclusionOur findings indicate CCAT1 may play a role in regulating autophagy by sponging miR‐181a‐5p in HCC.
Exosomal microRNA and stroke: A review
Authors: Ghoreishy Abdolreza , Khosravi Alireza , Ghaemmaghami Amir 
Published: June 2019

AbstractBlood vessels rupture or occlusion in brain results in stroke. Stroke is the major reason for mortality and dysfunction worldwide. Despite several attempts, there are no any approved therapeutic approaches for stroke subjects. The most neuroprotective agents showed the positive effects in preclinical reports, while there are no significant therapeutic impacts in the clinical trials. MicroRNAs (miRNAs) are small noncoding RNAs which involved in the modulation of a variety of cellular and molecular pathways. Given that deregulation of these molecules is related to initiation and progression of stroke. Exosomes are nano‐carriers which are able to transfer different cargos such as miRNAs to recipient cells. Increasing evidence revealed that exosomal miRNAs are one of very important factors which are involved in the pathogenesis of stroke. Hence, more understanding about the role of exosomal miRNAs in stroke pathogenesis could contribute in discovering and developing new therapeutic approaches. Moreover, it has been proved the exosomal miRNAs could be used as noninvasive biomarkers in diagnosis and monitoring response to therapy in subjects with stroke. Herein for first time, we summarized different exosomal miRNAs involved in pathogenesis of stroke.
CRISPR‐Cas system: Toward a more efficient technology for genome editing and beyond
Authors: Ahmadzadeh Vahideh , Farajnia Safar , Baghban Roghayyeh , Rahbarnia Leila , Zarredar Habib 
Published: June 2019

AbstractGenome engineering technology is of great interest for biomedical research that enables scientists to make specific manipulation in the DNA sequence. Early methods for introducing double‐stranded DNA breaks relies on protein‐based systems. These platforms have enabled fascinating advances, but all are costly and time‐consuming to engineer, preventing these from gaining high‐throughput applications. The CRISPR‐Cas9 system, co‐opted from bacteria, has generated considerable excitement in gene targeting. In this review, we describe gene targeting techniques with an emphasis on recent strategies to improve the specificities of CRISPR‐Cas systems for nuclease and non‐nuclease applications.
Upregulation of miR‐675‐5p induced by lncRNA H19 was associated with tumor progression and development by targeting tumor suppressor p53 in non–small cell lung cancer
Authors: Zheng Zi‐Hui , Wu Dong‐Mei , Fan Shao‐Hua , Zhang Zi‐Feng , Chen Gui‐Quan , Lu Jun 
Published: June 2019

AbstractLung cancer is the main cause of cancer‐related death, and the proportion of non–small cell lung cancer (NSCLC) on lung cancer is 85%, while more than 80% lung cancer patients are diagnosed with chronic obstructive pulmonary disease (COPD). In this study, we aimed to explore the potential mechanism of COPD induced NSCLC. Luciferase assay and reverse transcription‐polymerase chain reaction (RT‐PCR) were conducted to study the regulatory relationship between P53 and microRNA‐675 (miR‐675). Real‐time PCR, Western‐blot analysis, and MTT assay were performed to explore the impact of H19 and miR‐675 in the signaling pathway involved in COPD induced NSCLC. In NSCLC patients with COPD, H19 and miR‐675 levels were strikingly upregulated while P53 level was significantly downregulated. P53 was identified as a target gene of miR‐675, and H19 remarkably upregulated miR‐675, while H19 siRNA notably inhibited miR‐675. In addition, miR‐675 and H19 dramatically suppressed the expression of P53 and Bax while inducing the expression of Bcl‐2. Finally, H19 and miR‐675 induced proliferation of A549 and MRC‐5 cells. These finding indicated that COPD (hypoxia)‐induced H19 promoted expression of miR‐675 associated with NSCLC though target apoptosis‐related protein P53, BAX, and Bcl‐2.
Long noncoding RNA OIP5‐AS1 aggravates cell proliferation, migration in gastric cancer by epigenetically silencing NLRP6 expression via binding EZH2
Authors: Bai Yunlei , Li Sheng 
Published: June 2019

AbstractThe critical role of long noncoding RNAs (lncRNAs) in the development of multiple cancers has been revealed either functioning as a tumor initiator or a cancer suppressor. A widely recognized OIP5 antisense RNA 1 (lncRNA OIP5‐AS1) has been validated to be an essential regulator of the tumorigenesis of various malignancies. Whereas, the potential role and the exact mechanism of lncRNA OIP5‐AS1 by which OIP5‐AS1 mediates gastric cancer (GC) progression remains vague. Therefore, first our work probed its expression levels in GC cell lines and related normal cells by real‐time quantitative polymerase chain reaction. The heightened level of OIP5‐AS1 was detected in GC cell lines. In terms of its cellular effects, we performed a series of functional experiments and as presented in the assays, the proliferative potential and motility was diminished. However, more apoptotic cells were induced with the introduction of OIP5‐AS1 silencing. Meanwhile, higher Nod‐like receptor pyrin domain‐containing protein 6 (NLRP6) and enhancer of zeste homolog 2 (EZH2) expression in the GC cells was monitored. Besides, OIP5‐AS1 was disclosed to locate mainly in the nucleus. In terms of mechanism, OIP5‐AS1 directly bound to EZH2 and obstructed NLRP6 expression, speeding up GC progression.
MicroRNA‐323‐3p promotes myogenesis by targeting Smad2
Authors: Qin Jin , Sun Yunmei , Liu Shuge , Zhao Rui , Zhang Qiyue , Pang Weijun 
Published: June 2019

AbstractSkeletal muscle is an important and complex organ with multiple biological functions in humans and animals. Proliferation and differentiation of myoblasts are the key steps during the development of skeletal muscle. MicroRNA (miRNA) is a class of 21‐nucleotide noncoding RNAs regulating gene expression by combining with the 3′‐untranslated region of target messenger RNA. Many studies in recent years have suggested that miRNAs play a critical role in myogenesis. Through high‐throughput sequencing, we found that miR‐323‐3p showed significant changes in the longissimus dorsi muscle of Rongchang pigs in different age groups. In this study, we discovered that overexpression of miR‐323‐3p repressed myoblast proliferation and promoted differentiation, whereas the inhibitor of miR‐323‐3p displayed the opposite results. Furthermore, we predicted Smad2 as the target gene of miR‐323‐3p and found that miR‐323‐3p directly modulated the expression level of Smad2. Then luciferase reporter assays verified that Smad2 was a target gene of miR‐323‐3p during the differentiation of myoblasts. These findings reveal that miR‐323‐3p is a positive regulator of myogenesis by targeting Smad2. This provides a novel mechanism of miRNAs in myogenesis.
The inhibition of BDNF/TrkB/PI3K/Akt signal mediated by AG1601 promotes apoptosis in malignant glioma
Authors: Yin Hongqiang , Jiang Yu , Zhang Yinguo , Ge Hui , Yang Zhuo 
Published: June 2019

AbstractMalignant glioma is the most aggressive primary brain tumor and has a poor survival rate. Even if extensive methods are preformed to treat glioma, the mortality rate is still very high. It is necessary for discovering and developing new drugs for malignant glioma treatment. AG1601 is one of AG‐series drugs, including AG1031 and AG1503, and it has been optimized on the original basis. In our study, we found that AG1601 markedly inhibited proliferation and promoted C6 glioma cell apoptosis in vitro. AG1601 also reduced the size and weight of glioma in vivo. The growth ability of glioma was significantly inhibited after treatment with AG1601. It also showed that the expression levels of BDNF/TrkB/PI3K/Akt signal related proteins were obviously decreased in C6 glioma cells after treatment with AG1601 in vivo and in vitro. We also found that BDNF, as the activator of BDNF/TrkB/PI3K/Akt signal, reversed the anti‐proliferation and pro‐apoptosis of C6 glioma cells caused by AG1601. K252a, a specific inhibitor of TrkB, and AG1601 in combination aggravated C6 glioma cell apoptosis. These results indicate that AG1601 has good effects on the anti‐proliferation and pro‐apoptosis of malignant glioma via BDNF/TrkB/PI3K/Akt signal and could be considered as a potential drug in treating malignant glioma.
Association and in silico investigations of miR‐302c insertion/deletion variant as a novel biomarker with susceptibility to gastric cancer
Authors: Raad Mohammad , Bayat Amir , Sharafshah Alireza , Amiri Ali Zahedi , Zohour Mostafa Montazer , Ahmadvand Mohammad 
Published: June 2019

AbstractGastric cancer (GC) is the fifth most prevalent malignant tumor and the third most frequent cause of cancer mortality worldwide. rs199971565 is an insertion/deletion (INDEL) located in microRNA‐302c (miR‐302c) seed site, which may affect its function and biogenesis. There is no genetic association study investigating this INDEL with any disease till now. Thus, the current study was conducted to investigate the association of rs199971565 with susceptibility to GC in an Iranian population. In addition, in silico studies were performed to reveal the possible functional significance of this INDEL. A total of 378 subjects were genotyped through amplification refractory mutation system PCR (ARMS‐PCR) after DNA extraction from peripheral blood by the salting out procedure. Also, in silico analyses were performed through databases and web tools including MiRNASNP V2.0, miRWalk V2.0, miRTarBase, DAVID V6.8, RNAfold, PHDcleave, miRmap, and STarMir. Results revealed that there was an association between rs199971565 and the incidence risk of GC under a recessive (P = .04, odds ratio [OR] = 18.73; 95% confidence interval [CI] = 1.07‐326.95) model of inheritance. Also, compared to the Ins allele, the Del allele significantly increased the risk of GC (P = .01, OR = 2.02; 95% CI = 1.11‐3.66). Further analyses showed no significant association in age and sex between two study groups (P = .216 and P = .798, respectively). In conclusion, for the first time, this study indicated the association and in silico investigations of rs199971565 and suggested it as a novel INDEL biomarker located in the seed site of miR‐302c, which may have crucial roles in the susceptibility to GC and its incidence risk.
Analytical comparison between two hematological analyzer systems: Mindray BC‐5180 vs Sysmex XN‐1000
Authors: Wang Jing , Zhao Shengmei , Su Zhenguo , Liu Xiangdong 
Published: June 2019

AbstractBackgroundTo compare the Mindray BC‐5180 and Sysmex XN‐1000 instruments by analyzing the results of complete blood count in the external quality assessment in Shandong Province in 2018.MethodsIn the external quality assessment, 10 batches of quality control materials were issued throughout the year. The test items were WBC, RBC, Hb, PLT, and HCT. The laboratories using Mindray BC‐5180 and Sysmex XN‐1000 were screened, and the results were analyzed by t test, Passing‐Bablok regression analysis, and Bland‐Altman analysis.ResultsThirty‐six laboratories using Mindray BC‐5180 instruments and thirty‐six laboratories using Sysmex XN‐1000 instruments were screened, and the average difference between the two instruments results is not significant (P > 0.05, t test). Passing‐Bablok regression analysis showed that the 95% confidence interval of the regression equation interception of each test item included 0, and the 95% confidence interval of the slope contained 1, r > 0.98, which showed that the correlation is good. The Bland‐Altman analysis showed that both instruments had more than 95% of the points within the 95% consistency limit (WBC97.2%, RBC95.6%, PLT97.2%, Hb96.7%, HCT97.5%). Within the consistency limit, the absolute value of the difference between the Mindray BC‐5180 instrument and the Sysmex XN‐1000 instrument is WBC 0.14%, RBC 0.26%, PLT 2.7%, and Hb 1.9%. HCT is 0.69%, and the difference is clinically acceptable.ConclusionIt can be considered that the two instruments have good correlation and consistency, and the two instruments can replace each other.
Microtubule‐interfering agents, spindle defects, and interkinetochore tension
Authors: Qi Feifei , Zhou Jun , Liu Min 
Published: June 2019

AbstractMicrotubule‐interfering agents have been very useful both as biological tools in studying mitosis and as chemotherapeutic agents against cancer. It remains poorly understood how these agents converge on the spindle assembly checkpoint (SAC) to halt mitotic progression, while inhibiting microtubule dynamics by different mechanisms. Cells arrested at mitosis by various microtubule‐interfering agents exhibit strikingly different defects in the mitotic spindle. However, all the arrested cells possess the 3F3/2 phosphoepitope at the sister kinetochores of chromosomes, indicating the decrease of tension across the paired kinetochores. In addition, microtubule‐interfering agents result in a comparable reduction in the distance between sister kinetochores, suggesting that these agents decrease interkinetochore tension to similar degrees. Here, we discuss recent progress that suggests impairment of kinetochore‐microtubule attachment and reduction of interkinetochore tension as common mechanisms underlying the persistent SAC activation in response to diverse microtubule‐interfering agents.
Inhalation Aerosol Therapy in the Treatment of Chronic Rhinosinusitis: A Prospective Randomized Study
Authors: Velepič Marko , Manestar Dubravko , Perković Ivona , Škalamera Dunja , Braut Tamara 
Published: June 2019

AbstractThe purpose of the study was to compare treatment of chronic rhinosinusitis (CRS) with topical glucocorticoids and saline irrigation versus aerosol inhalation therapy. Patients diagnosed with CRS were randomly divided into 2 groups. In the first group, patients were treated with topical glucocorticoids (mometasone furoate, 100 µg in each nostril once daily) and saline irrigation (150 mL twice a day) for 2 weeks. In the second group, patients were treated with inhalation aerosol therapy composed of essential oils, saline, glucocorticoids, and antibiotics, once daily 5 times per week (Monday through Friday), for 2 weeks. The effect of the treatments was compared between the 2 groups. In the first group there was no significant improvement in the Glasgow Health Status Inventory (GHSI) (P = .29). In the second group the improvement in GHSI score was significant (P = .037). It was shown that in the first group the Glasgow Benefit Inventory score was significantly lower than in the second group (P = .002), which means that the improvement in the health status after the therapy was better in the second group. A Lund‐Kennedy score showed statistical improvement in both groups (both P < .001). Improvement was also compared between the groups. The results were not significant (P = .11). The authors concluded that, in this preliminary research, inhalation aerosol therapy composed of essential oils, saline, glucocorticoids, and antibiotics led to better subjective results than intranasal glucocorticoid therapy and saline irrigation in the treatment of CRS. Further investigations with more participants, longer periods of treatment, and different validation tools are needed to confirm our results.
Optimization of the 3D electro‐Fenton process in removal of acid orange 10 from aqueous solutions by response surface methodology
Authors: Mohammadi Hamed , Alinejad Abdolazim , Khajeh Mahsa , Darvishmotevalli Mohammad , Moradnia Maryam , Tehrani Ashraf Mazaheri , Hosseindost Gholamreza , Zare Mohammad Reza , Mengelizadeh Nezamaddin 
Published: June 2019

AbstractBACKGROUNDIn recent decades, rapid population growth and industrial development have led to the release of toxic compounds into water resources. Several technologies have been proposed to address the problem, and among them, the 3D electro‐Fenton process has found great popularity due to its high efficiency and simple operation. In the 3D electro‐Fenton process, the type of particle electrode is one of the most important factors owing to its impact on the reduction of energy consumption and generation of reactive species. In the present study, the optimization of the 3D electro‐Fenton process coupled with iron‐coated nickel foam (NF‐Fe), as a new particle electrode, in the removal of acid orange 10 was investigated using response surface methodology (RSM).RESULTSThe RSM results showed that the quadratic model has a high R2 and high F‐value based on ANOVA analysis. The optimum efficiency of dye removal (99.15%) was obtained at pH 5.6, an NF‐Fe dosage of 0.68 g L−1, a time of 34.6 min, and current density of 23.62 mA cm−2. Comparative tests showed the high efficiency of the 3D electro‐Fenton process compared to other electrochemical systems. Quenching experiments demonstrated the production of different reactive species in the 3D process. The stability experiment proved that the NF‐Fe has a high potential to improve the long‐term degradation of dye.CONCLUSIONSDue to its high efficiency, production of more reactive species and excellent stability of the NF‐Fe electrode, this 3D electro‐Fenton process is an excellent option for removal of dye from aqueous solutions. © 2019 Society of Chemical Industry
Comprehensive review and future perspectives on the photocatalytic hydrogen production
Authors: Corredor Juan , Rivero María J , Rangel Carmen M , Gloaguen Frederic , Ortiz Inmaculada 
Published: June 2019

AbstractHydrogen represents a renewable energy alternative that may positively contribute to get over the global energy crisis while at the same time reducing its environmental burden. Overcoming the challenge of reaching this potential could be helped by careful choice of hydrogen (H2) sources. Photocatalytic generation of H2, although a minor alternative, appears to be a very good option at the time that liquid wastes are being degraded; therefore, this approach has given rise to an increasing number of interesting studies.
Here, we aim to provide an integrated overview of the different photocatalytic, heterogeneous, homogeneous and hybrid systems. First, we categorize the units and mechanisms that take part in the photocatalytic process, and secondly we analyze their role and draw comparative conclusions. Thus, we analyze the role of (i) the electron source to carry out proton reduction, (ii) the proton source, which can be free protons in the medium or a proton donor compound, (iii) the catalyst nature and concentration, and (iv) the photosensitizer nature and concentration. We also provide an analysis of the influence of the solvent, especially in homogenous systems as well as the influence of pH. We provide a comparison of the photocatalytic performance, highlighting the advantages and disadvantages, of different systems. Thus, this review is, on the one hand, an update on the state of the art of photocatalytic generation of H2 from a full perspective that integrates homogeneous, heterogeneous and hybrid systems, and, on the other, a source of useful information for future research. © 2019 Society of Chemical Industry