bluuebunny/crossref_metadata_2025_split huggingface dataset:
Proteomic characterization of primary cultured myocytes in a fish model at different myogenesis stages
Authors: Millan-Cubillo Antonio F. , Martin-Perez Miguel , Ibarz Antoni , Fernandez-Borras Jaume , Gutiérrez Joaquim , Blasco Josefina 
Published: October 2019

AbstractMyogenesis is a complex two-phase process of proliferation and differentiation, which seems to be greatly conserved in vertebrates. For the first time in fish, we identify the changes that occur in the proteome during this process in a gilthead sea bream (Sparus aurata) myocyte primary cell culture (on days 4, 8 and 12), using 2-D gel electrophoresis and LC-MS/MS. A significant increase of myogenin expression at day 8 marked the transition from proliferation to differentiation. Of the 898 spots in the proteome analysis, the 25 protein spots overexpressed on day 4 and the 15 protein spots overexpressed on day 8 indicate the end of proliferation and the beginning of differentiation, respectively. Proliferation was characterized by enrichment of proteins involved in actin cytoskeleton remodelling and in cellular metabolic processes (transcription, ubiquitination, response to stress and glucose metabolism). During differentiation, 41 proteins were overexpressed and 51 underexpressed; many of them related to biosynthetic processes (RNA and protein synthesis and folding, and pentose pathways), terminal myotube formation and muscle contraction. The main cellular processes of both phases of muscle development in fish are similar with those observed in mammals but extended in time, allowing sequential studies of myogenesis.
The role of EUS in diagnosis and treatment of liver disorders
Authors: Campos Sara , Poley Jan-Werner , van Driel Lydi , Bruno Marco J. 
Published: October 2019

Abstract
Background and aim Transabdominal ultrasound (US), computed tomographic scanning (CT) and magnetic resonance imaging (MRI) are established diagnostic tools for liver diseases. Percutaneous transhepatic cholangiography is used to perform hepatic interventional procedures including biopsy, biliary drainage procedures, and radiofrequency ablation. Despite their widespread use, these techniques have limitations. Endoscopic ultrasound (EUS), a tool that has proven useful for evaluating the mediastinum, esophagus, stomach, pancreas, and biliary tract, has an expanding role in the field of hepatology complementing the traditional investigational modalities. This review aimed to assess the current scientific evidence regarding diagnostic and therapeutic applications of EUS for hepatic diseases.
Comparison of small-bowel colon capsule endoscopy system to conventional colonoscopy for the evaluation of ulcerative colitis activity
Authors: Adler Samuel N. , González Lama Yago , Matallana Royo Virginia , Suárez Ferrer Cristina , Schwartz Avraham , Bar-Gil Shitrit Ariella 
Published: October 2019

Abstract
Background and aims Diagnosis and monitoring of ulcerative colitis (UC) includes conventional colonoscopy. This procedure is invasive and does not exclude small-bowel Crohn’s disease (CD). Current therapeutic goals include mucosal healing which may lead to an increased number of endoscopic procedures in many patients. The small-bowel colon capsule endoscopy (SBC-CE) system visualizes the small bowel and colon. The aim of this study was to evaluate the performance and adverse events of SBC-CE in patients with UC.
Methods This was a prospective, feasibility study involving two study sites. Patients with active UC underwent SBC-CE and colonoscopy. Kappa statistics were performed to assess the agreement between SBC-CE and colonoscopy. Adverse events (AEs) data were collected throughout and following the procedure.
Results In total, 30 consecutive patients were recruited, and 23 of those were included in the final analysis. For the primary end point, evaluation of the extent of UC disease in the colon, the percent agreement between SBC-CE and colonoscopy was moderate (56.5 %); kappa coefficient 0.42. The percent agreement between SBC-CE and colonoscopy for UC disease activity, based on Mayo endoscopic sub-score, was 95.7 %; kappa coefficient 0.86. Disease activity in the more proximal small bowel was detected in two patients with SBC-CE. No SBC-CE device-related AEs were reported.
Conclusions When comparing SBC-CE to conventional colonoscopy, there was a moderate agreement for the extent of UC disease and a very good overall agreement between the two modalities for UC disease activity.
Endoscopic ultrasound-guided fine-needle biopsy is superior to fine-needle aspiration in assessing pancreatic neuroendocrine tumors
Authors: Leeds John S , Nayar Manu K. , Bekkali Noor L.H. , Wilson Colin H. , Johnson Sarah J. , Haugk Beate , Darne Antony , Oppong Kofi W. 
Published: October 2019

Abstract
Background and study aims Pancreatic neuroendocrine tumors (PanNETs) outcomes are dependent upon grading by Ki67. This study compared endoscopic ultrasound-guided fine-needle biopsy (EUS-FNB) to fine-needle aspiration (FNA) in assessing PanNETs.
Patients and methods All pancreatic histology for PanNET between January 2009 and June 2017 was included if EUS sampling was performed prior to surgical resection. Ki67 and grade from FNA and FNB samples was compared to surgical histology using correlation coefficient and kappa values. Subgroup analysis was performed for purely solid lesions, lesions < 2 cm and FNB needle type.
Results One hundred sixity-four patients had PanNET of which 57 underwent surgical resection. Thirty-five lesions underwent FNA and 26 FNB (4 had both) confirming PanNET. 23/ of 35 FNA samples reported Ki67/grading compared to all 26 FNB samples (P = 0.0006). Compared to surgical histology, Ki67 on FNA correlated poorly overall (r = –0.08), in solid lesions (r = –0.102) and lesions < 2 cm (r = –0.149) whereas FNB correlated moderately overall (r = 0.65), in solid lesions (r = 0.64) and lesions < 2 cm (r = 0.61). Tumor grade showed poor agreement (kappa) with FNA overall (0.026), in solid lesions (0.044) and lesions < 2 cm (0.00) whereas FNB showed moderate-good agreement overall (0.474), in solid lesions (0.58) and lesions < 2 cm (0.745). Fork-tip FNB needles Ki67 showed strong correlation with surgical histology (r = 0.788) compared to reverse bevel FNB needles (r = 0.521). Both FNB needles showed moderate agreement with tumor grade.
Conclusion FNB samples were significantly more likely than FNA to provide adequate material for Ki67/grading and showed a closer match to surgical histology. FNB needle types require prospective investigation.
„Versorgungsforscher übernehmen Verantwortung“
Authors: Schmitt Jochen , Klinkhammer-Schalke Monika 
Published: October 2019

Liebe Kolleginnen und Kollegen, sehr geehrte Damen und Herren,die Versorgungsforschung hat sich in den vergangenen Jahren in Deutschland zu einer tragenden Säule der medizinischen Forschung entwickelt. Sie kann heute auch hierzulande als etabliert gelten. Dies verdanken wir klugen Förderprogrammen des Bundesministeriums für Bildung und Forschung (BMBF) des Bundesministeriums für Gesundheit (BMG) und nicht zuletzt dem Innovationsfonds des Gemeinsamen Bundesausschusses (G-BA).
Gastric Pneumatosis: Emphysematous Gastritis versus Gastric Emphysema
Authors: Paliwal Amit Kumar , Girdhar Sachin , Pattanayak Somali , Kumar Brajesh 
Published: October 2019

AbstractPresence of air in the wall of the stomach is known as gastric pneumatosis. It may be associated with a benign condition like gastric emphysema (GE) to life threatening condition emphysematous gastritis (EG). Differentiation between two entities based on clinical presentation, predisposing factors, and radiological findings is important as EG has more complications and higher rates of mortality. The treatment in GE is conservative while treatment in EG is evolving. We present a case of a diabetic patient who developed EG following abdominal surgery and managed conservatively with favorable outcome.
Composites Associated with Pulp-Protection Material: Color-Stability Analysis after Accelerated Artificial Aging
Authors: Cruvinel Diogo Rodrigues , Garcia Lucas da Fonseca Roberti , Consani Simonides , Pires-de-Souza Fernanda de Carvalho Panzeri 
Published: October 2019

ABSTRACTObjectives: This study assessed the color stability of two composites associated with two pulp protectors submitted to accelerated artificial aging (AAA).Methods: 60 test specimens were made with 0.5 mm of protection material (calcium hydroxide - CH or glass ionomer cement - GIC) and 2.5 mm of restoration material (Concept or QuixFil) and divided into 3 groups (n=10) according to the type of protection material/composite, and the control group (no protection). After polishing, color readings were obtained with a spectrophotometer (PCB 6807 Byk Gardner) before and after AAA for 384 hours, and L*, a*, and b* coordinates and total color variation (ΔE) were analyzed (2-way ANOVA, Bonferroni, α=05).Results: Composites placed on CH presented lower L* levels than those on GIC, which presented higher L* values than the control group and lower b* values than those of the CH group. The Concept composite presented higher ΔE levels for all groups, differing statistically from QuixFil, except when placed on GIC.Conclusions: It was concluded that the protection material could affect the color stability and AAA is a factor that enhances this effect, depending on the type of composite used. (Eur J Dent 2010;4:6-11)
Monomer Release from Resin Based Dental Materials Cured With LED and Halogen Lights
Authors: Ak Asli Topaloglu , Alpoz A. Riza , Bayraktar Oguz , Ertugrul Fahinur 
Published: October 2019

ABSTRACTObjectives: To measure the release of TEGDMA and BisGMA from two commercially available composite resins; Filtek Z 250 (3M ESPE, Germany), Leaddent (Leaddent, Germany) and two fissure sealants; Helioseal F (3M ESPE, Germany) Enamel Loc (Premiere Rev, USA) over 1, 3 and 7 days after polymerization with standard quartz-tungsten halogen Coltolux II (QHL) (Coltene Switzerland) and a standard blue light emitting diode Elipar Freelight 2 (3M ESPE, Germany).Methods: 9 samples of each material were placed in disc shaped specimens in 1 mm of thickness and 10 mm in diameter (n=36). Each material was polymerized using LED for 20 s (n=12), 40 s (n=12) and halogen for 40 s (n=12), respectively. High Performance Liquid Chromatography (HPLC) was used to measure the amount of monomers released over 1, 3 and 7 days. Data was analyzed using one way ANOVA and Bonferroni test for multiple comparisons with a significance level of .05.Results: LED 20 sec group showed the highest release of monomers at 1, 3 and 7 days in sealant groups. Halogen 40 sec group resulted highest release of monomers for Leaddent at all time intervals (P<.05)Conclusions: Efficiency of the curing unit and applying the recommended curing time of the light activated resin based dental materials is very important to protect the patient from potential hazards of residual monomers. (Eur J Dent 2010;4:34-40)
The REVision Using Imaging to Guide Staging and Evaluation (REVISE) in ACL Reconstruction Classification
Authors: de SA Darren , Crum Raphael J. , Rabuck Stephen , Ayeni Olufemi , Bedi Asheesh , Baraga Michael , Getgood Alan , Kaar Scott , Kropf Eric , Mauro Craig , Peterson Devin , Vyas Dharmesh , Musahl Volker , Lesniak Bryson P. 
Published: October 2019

AbstractRevision anterior cruciate ligament (ACL) procedures are increasing in incidence and possess markedly inferior clinical outcomes (76% satisfaction) and return-to-sports (57%) rates than their primary counterparts. Given their complexity, a universal language is required to identify and communicate the technical challenges faced with revision procedures and guide treatment strategies. The proposed REVision using Imaging to guide Staging and Evaluation (REVISE) ACL (anterior cruciate ligament) Classification can serve as a foundation for this universal language that is feasible and practical with acceptable inter-rater agreement. A focus group of sports medicine fellowship-trained orthopaedic surgeons was assembled to develop a classification to assess femoral/tibial tunnel “usability” (placement, widening, overlap) and guide the revision reconstruction strategy (one-stage vs. two-stage) post–failed ACL reconstruction. Twelve board-certified sports medicine orthopaedic surgeons independently applied the classification to the de-identified computed tomographic (CT) scan data of 10 patients, randomly selected, who failed ACL reconstruction. An interclass correlation coefficient (ICC) was calculated (with 95% confidence intervals) to assess agreement among reviewers concerning the three major classifications of the proposed system. Across surgeons, and on an individual patient basis, there was high internal validity and observed agreement on treatment strategy (one-stage vs. two-stage revision). Reliability testing of the classification using CT scan data demonstrated an ICC (95% confidence interval) of 0.92 (0.80–0.98) suggesting “substantial” agreement between the surgeons across all patients for all elements of the classification. The proposed REVISE ACL Classification, which employs CT scan analysis to both identify technical issues and guide revision ACL treatment strategy (one- or two-stage), constitutes a feasible and practical system with high internal validity, high observed agreement, and substantial inter-rater agreement. Adoption of this classification, both clinically and in research, will help provide a universal language for orthopaedic surgeons to discuss these complex clinical presentations and help standardize an approach to diagnosis and treatment to improve patient outcomes. The Level of Evidence for this study is 3.
Results of Using a “2-in-1” Single-Stage Revision Total Knee Arthroplasty for Infection with Associated Bone Loss: Prospective 2-Year Follow-Up
Authors: Holland George , Brown Gavin , Goudie Stuart , Brenkel Ivan , Walmsley Philip J. 
Published: October 2019

AbstractProsthetic joint infections provide a complex challenge for management, owing to their often difficult diagnoses, need for multiple surgeries, and increased technical and financial requirements. The ‘2 in 1’ single-stage approaches have been recently advocated in the field of arthroplasty on account of their reduction in risks, costs, and complications. The aim of our study was to investigate the outcomes of this variant of single-stage revision, which is used in the setting of infection following primary total knee replacement (TKR) and associated bone loss. Prospective data were collected from all patients presenting with an infection following primary TKR over an 8-year period (2009–2017). We examined revision procedures that were undertaken as a single-stage procedure and had bone loss present. Patients were followed-up for evidence of recurrent infection. Functional assessments were conducted using range of motion, Oxford Knee Score (OKS), American Knee Society Score (AKSS), and Short Form-12 (SF-12) survey. Twenty-six patients were included in the analysis, two of whom had previously failed 2 stage revision; another three among them had failed debridement, antibiotics, irrigation, and implant retention procedures. The mean age was 72.5 years, mean body mass index was 33.4, and median American Society of Anesthesiologists (ASA) physical status classification was 2. The mean time to revision was 3.5 years (3 months to 12 years). Six patients had actively been discharging sinuses at the time of surgery. Only 4/26 patients possessed no positive microbiological cultures from deep tissue samples or joint aspirates. One patient was afflicted with a recurrence of infection. This patient did not require further surgery and was successfully treated with the help of long-term antibiotic suppression. There were statistically significant improvements in both the pain component of AKSS scores (preoperative 4.3 to postoperative 32.4) and the functional component of AKSS scores (preoperative 10.7 to postoperative 15.7). There was no significant improvement in flexion; however, mean extension (increased from 18.5 to 6.9 postoperative) and total range of motion (increased from 69.2 preoperative to 90.3 postoperative) both showed statistically significant improvements. The use of “2-in-1” single-stage revision can be considered as an effective option for treating infection following TKR and associated bone loss.
A Comparative Study of Orotracheal Intubation Guided by Airtraq and McCoy Laryngoscope in Patients with Rigid Cervical Collar In-situ Simulating Cervical Immobilization for Traumatic Cervical Spine Injury
Authors: Diwan Akhil , Purohit Shobha 
Published: October 2019

Abstract
Background Immobilization of cervical spine is of utmost importance in patients with cervical injury, making intubation a difficult task due to the application of cervical collar. This study was done to assess which laryngoscope (Airtraq or McCoy) is better for intubation and prevents the deleterious effects of hypoxia by comparing the intubation time.
Methods A prospective interventional randomized study was undertaken in 60 adult patients of American Society of Anesthesiologists (ASA) grade I and II, aged between 20 and 50 years, scheduled for various surgical procedures requiring tracheal intubation for anesthesia. Patients were randomly allocated to undergo intubation with either the Airtraq (Group A; n = 30) laryngoscope or McCoy (Group B; n = 30). Patients were intubated following the standard anesthetic protocol, and the differences in duration of intubation, changes in the hemodynamic parameters in response to intubation, modified intubation difficulty score, and airway complications between the Airtraq and the McCoy laryngoscope were compared.
Results Overall intubation success rates were 100% with both devices and a similar number of intubation attempts were required. Though the mean time required for successful intubation was less with the Airtraq (25.2 ± 5.11 seconds) than the McCoy laryngoscope (27.3 ± 4.47 seconds); it was statistically insignificant (p = 0.14). Intubation difficulty score and ease of insertion were significantly less in Airtraq laryngoscope when compared with McCoy laryngoscope. Increase in the heart rate and mean arterial pressure was significantly more just after intubation with McCoy in comparison with Airtraq laryngoscope. However, the frequencies of airway complications were similar.
Conclusion Intubation time despite being lesser with the Airtraq than the McCoy laryngoscope was statistically insignificant, and overall success rates between the two devices were similar.
Effect of Previous Calcium Hydroxide Dressing on the Sealing Properties of the New Endodontic Cement Apical Barrier
Authors: Bidar Maryam , Disfani Reza , Gharagozlo Salman , Rouhani Armita , Forghani Maryam 
Published: October 2019

ABSTRACTObjectives: Using the fluid filtration method, an in vitro study was conducted to evaluate the effects of medication with calcium hydroxide on the sealing ability of the New Endodontic Cement (NEC) apical barrier in the short and long terms. Methods: Fifty extracted single rooted teeth were prepared and apical resorption produced using acid sulfuric for four days. The teeth were randomly divided into two experimental groups (n=20) and two control groups. In group 1, calcium hydroxide was placed into all canals for one week and in group 2, no medication was used. In both groups, a 4 mm NEC apical plug was placed in the canals and the remaining portion of the canals was filled with gutta-percha. The amount of microleakage of all samples was evaluated after one week and three months. The data were statistically analyzed using two-way ANOVA. Results: There was no significant difference between the two groups in either time period (P>.05). In both group 1 and group 2, microleakage increased after three months but this increase was not statistically significant (P>.05). Conclusions: According to the result of this study, medication with calcium hydroxide had no adverse effect on the short- and long-term sealing properties of an NEC apical plug. (Eur J Dent 2011;5:260-264)
Characteristics of occlusion in primary dentition of preschool children of Udaipur, India
Authors: Hegde Sapna , Panwar Sunil , Bolar Dinesh Rao , Sanghavi Mitesh Bharat 
Published: October 2019

ABSTRACTObjective: The present study aimed to study the occlusion characteristics of the primary dentition in a group of 3-5 year-old Indian children and the differences with age in the same group.Methods: The study was a cross-sectional survey based on examination of the primary dentition of 200 preschool children aged 3-5 years who were selected from nursery schools in Udaipur, India, using a stratified random sampling technique. The study group was assessed for the several occlusal parameters, which included primary molar and canine relationship, degree of overjet and overbite, anterior and posterior crossbite, and the presence or absence of physiologic spaces and crowding.Results: Flush terminal plane was more common at 3-4 years of age, mesial step at 4-5 years, and class I canine relationship in both age groups. The prevalence of overjet less than 1 mm and overjet exceeding 1 mm was almost comparable in both groups. Overbite with less than 30% overlap was most frequently observed in both age groups, with 30-60% overlap following closely behind. Physiologic and primate spacing were observed in less than half of the children examined, while a small percentage displayed the presence of either single-segment or two-segment crowding.Conclusion: The present study provided insight into the state of dentition, occlusal pattern, spacing, and crowding in the primary dentition of Indian children of Udaipur. (Eur J Dent 2012;6:51-55)
Cell mechanosensing is regulated by substrate strain energy rather than stiffness
Authors: Panzetta Valeria , Fusco Sabato , Netti Paolo A. 
Published: October 2019

Significance
In the long-lasting debate about the duality of the force/deformation cell sensing mechanism we focused attention on the question of whether the cell is sensitive to the stiffness or to the energy required to deform the surrounding material. To address the role of strain energy as a regulator of cell mechanosensing we investigated the cytoskeleton mechanics of cells seeded on linearly elastic substrates, applying several levels of deformation before and after cell seeding. The results indicated that cells recognize the prestrain, exhibiting a stiffer cytoskeleton on stretched material compared to cells seeded on unstretched substrate. This observation clearly suggests that cell mechanosensing is not mediated only by the stiffness but also by the deformation energy of the substrate.
Muscle acetylcholine receptor conversion into chloride conductance at positive potentials by a single mutation
Authors: Cetin Hakan , Epstein Max , Liu Wei W. , Maxwell Susan , Rodriguez Cruz Pedro M. , Cossins Judith , Vincent Angela , Webster Richard , Biggin Philip C. , Beeson David 
Published: October 2019

Significance
We report on a single mutation in the α1-subunit M2 helix (p.α1Leu251Arg) of the muscle acetylcholine receptor (AChR) found in a patient with congenital myasthenic syndrome (CMS) that is shown to convert the AChR into chloride conductance at positive potentials. Constriction of the channel pore with partial desolvation and stabilization of the permeating chloride ions by the arginine residues is revealed as the underlying mechanism. This article is of general interest because it describes a mechanism for the transformation of the muscle AChR into an inhibitory channel, and presents a report of charge selectivity conversion in association with a naturally occurring single mutation. Our findings might also give explanation to a pathomechanism in CMS.