bluuebunny/crossref_metadata_2025_split huggingface dataset:
Considerations from the 2017 IMFAR Preconference on Measuring Meaningful Outcomes from School‐Age to Adulthood
Authors: Bal Vanessa H. , Hendren Robert L. , Charman Tony , Abbeduto Leonard , Kasari Connie , Klinger Laura Grofer , Ence Whitney , Glavin Tara , Lyons Gregory , Rosenberg Erin 
Published: October 2018

The autism spectrum disorder (ASD) research community is increasingly considering the importance of measuring outcomes that are meaningful to individuals with ASD and their families. The 2017 IMFAR preconference aimed to gain the perspectives of how to define and measure “meaningful outcomes” from 280 participants, including people with ASD and their families, service providers, and researchers. Six themes were identified: (a) the definition of “outcome” varies by context and perspective; (b) the need to broaden the scope of what researchers measure; (c) the need for new assessment tools; (d) the need to expand data analytic methods; (e) where to focus (with emphasis on considering different developmental stages and aspects of diversity); and (f) a need for community partnerships to bridge research and daily practice. The challenge that the research community now faces is how to move the evidence base for clinical practice forward while keeping alive the divergence of views and considerations that are relevant for thinking about complex outcomes for the highly heterogeneous group of individuals with ASD. This commentary provides recommendations, with an emphasis on lifespan viewpoints that encompass individual strengths and preferences. Autism Research 2018, 11: 1446–1454. © 2018 International Society for Autism Research, Wiley Periodicals, Inc.Lay SummaryThe 2017 IMFAR preconference aimed to gain the perspectives of how to define and measure “meaningful outcomes” from a variety of stakeholders. This commentary outlines the six themes identified from keynote and panel presentations and audience‐participated discussions. Recommendations are made to emphasize perspectives that look across the lifespan and encompass individual strengths and preferences.
HMGB1 knock‐down promoting tumor cells viability and arrest pro‐apoptotic proteins via Stat3/NFκB in HepG2 cells
Authors: Alduais Salah , Alduais Yaser , Wu Xiaolei , Li Haosen , Mao Jing 
Published: October 2018

AbstractBackground/Aim: High mobility group box 1 protein (HMGB1) is functionally dynamic and pleiotropic molecule, it has the potential to promote both cell survival and death by regulating multiple signaling pathways, including inflammation and apoptosis. This study aimed at investigating the role of silencing HMGB1 on tumor cells apoptosis and pro‐inflammatory proteins expression in hepatocellular HepG2 cancer cells. Methods: HepG2 cells was transfected with si‐RNA HMGB1, and the effect on pro‐apoptotic proteins expressions like Bax, Bcl2, and pro‐inflammatory cytokines like, p65‐NFκB, and Cyclooxygenase‐2 (Cox2) was assessed using western blot, and also cells apoptosis and proliferation was assessed using annexin V FITC and Calcien AM expression in flow cytometry and fluorescence. Results: HMGB1 silencing was found significantly increase tumor cells viability with significant decrease of pro‐apoptotic proteins, also antiapoptotic protein Bcl2 was significantly up‐regulated, which suggests a possible role in restricting apoptosis. Furthermore, HMGB1 knocked down found to inhibit Stat3 phosphorylation and significantly affect NFkB p65/Cox2 expression which suggests a link between HMGB1 and Stat3 activation. Our results revealed that HMGB1 knocked down may suppress cells apoptosis and enhance HepG2 cells viability via NFkB/Cox2 and Stat3. © 2018 BioFactors, 44(6):570–576, 2018
Synthesis of Trinucleotide Building Blocks in Solution and on Solid Phase
Authors: Suchsland Ruth , Appel Bettina , Müller Sabine 
Published: October 2018

AbstractWe have developed two methods, in solution and on solid phase, that give easy access to trinucleotide phosphoramidites capable of undergoing coupling reactions by the solid‐phase phosphoramidite approach. The solution protocol is characterized by application of 5′‐O‐dimethoxytrityl (DMT) and 3′‐O‐tert‐butyldimethylsilyl (TBDMS) as a pair of orthogonal protecting groups and 2‐cyanoethyl (CE) for protection of the phosphate. Starting with suitably functionalized monomers, synthesis proceeds in the 3′‐ to 5′‐direction, delivering the fully protected trinucleotide. The 3′‐O‐protecting group is cleaved followed by phosphitylation of the free 3′‐OH group. The solid‐phase protocol is based on standard phosphoramidite chemistry in conjunction with a dithiomethyl linkage connecting the 3′‐starting nucleoside to the polymer. The disulfide bridge can be cleaved under neutral conditions for release of the trinucleotide from the support preserving all other protecting groups. © 2018 by John Wiley & Sons, Inc.
Clinical follow up and the impact of the Paris system in the assessment of patients with atypical urine cytology
Authors: Northrup Victoria , Acar Behram Cenk , Hossain Mohammad , Acker Matthew R. , Manuel Eric , Rahmeh Tarek 
Published: October 2018

BackgroundUrinary cytology is routinely used in the diagnosis of urothelial neoplasms, with good sensitivity for high‐grade urothelial carcinoma (HGUC) but less so for low‐grade urothelial neoplasm (LGUN). There is significant interobserver and interinstitutional variability, especially for the atypical category. The Paris system for reporting urinary cytology (TPS) was introduced to better define the various categories, especially atypical cytology.MethodsWe retrospectively reviewed 630 atypia of undetermined significance (AUS) cases and reclassified them based on TPS. In total, 501 cases previously reported as negative for malignancy had their medical records reviewed to serve as negative controls.ResultsOf 630 AUS cases, 299 (47.5%) were reclassified as negative for HGUC (NHGUC), 313 (49.7%) as atypical urothelial cells (AUCs) and 18 (2.9%) as suspicious for HGUC (SHGUC). Based on our institution's previous reporting system, the rate of underlying or subsequent HGUC was 2.8% for AUS, and 0% for negative. When AUS cases were reclassified under TPS, the rates were 1.5% for NHGUC, 4.8% for AUC, and 0% for SHGUC. Review of medical records showed that patients with AUS were more likely to be followed‐up compared with those with negative urine cytology (77.8% compared with 54.3%), particularly those under the care of non‐urologists.ConclusionsAUS diagnosis is associated with more patient follow up compared with NEG urine particularly among non‐urologists. Reclassifying according to TPS results in significant reduction in the rate of AUS and thus unnecessary testing. This reduction however may be at the expense of slightly decreased detection rate of HGUC.
IgG4‐related disease of the paratestis with the scrotal fluid: A case report
Authors: Mochizuki Kunio , Hanai Yuki , Nakazawa Kumiko , Murata Shiori , Kasai Kazuki , Mochizuki Naoko , Tahara Ippei , Kondo Tetsuo 
Published: October 2018

AbstractIgG4‐related disease (IgG4‐RD) can affect various organs, and the pancreas and salivary gland are representative examples. We report a rare case of IgG4‐RD of the paratestis. A 74‐year‐old man presented with left scrotal swelling. Scrotopuncture drainage and cytology confirmed a clear, yellow retention liquid (130 mL) with many small, similar lymphocytes and a few plasmacytes. Many lymphoid cells were immunopositive for CD3 on a cell block section, indicating that a predominant type of lymphoid cells was T cell. There were also some CD20 immunopositive cells and a few IgG4 immunopositive cells. Two months later the left scrotal swelling had returned, and he underwent radical inguinal orchiectomy. Microscopically, there was considerable lymphoplasmacytic inflammatory infiltration, fibrosis and abundant IgG4 immunopositive cells in the paratesticular region. The histopathologic and immunohistochemistry findings were consistent with IgG4‐RD. However, the abundant T cells in the scrotal fluid complicated the cytological diagnosis in our case.
Generation and characterization of pathogenic Mab21l2(R51C) mouse model
Authors: Tsang Shun‐Wa , Guo Yanjiang , Chan Long‐Hei , Huang Yingyu , Chow King L. 
Published: October 2018

AbstractMAB21L2(R51C) is one of the five documented MAB21L2 mutations in human patients with bilateral eye malformations identified via whole exome sequencing. In addition to the eye abnormality, patients with MAB21L2
R51C/+ mutation also have skeletal dysplasia and intellectual disability. To evaluate the pathology of this mutant allele systematically in understanding the functional role of MAB21L2 in human development, we introduce the R51C mutation into the mouse genome by CRISPR/Cas9 system to generate a mouse model for detailed characterization. The Mab21l2
R51C/+ mice have eyeless phenotype and skeletal abnormalities. Micro‐computed tomography (micro‐CT) analysis showed the Mab21l2
R51C/+ mice have no eye balls but with two abnormal tissues underneath the brain. Histological analysis revealed that the early eye development in the mutant embryos is interrupted. In addition, Mab21l2
R51C/+ mice also have joint fusion phenotype; the humerus is fused with radius, whereas femur is fused with tibia. Limbs in the mutant animals are distinctly shorter than the wild type; and deltoid tuberosities in humeri are absent in these Mab21l2
R51C/+ mice. In summary, we showed that our Mab21l2
R51C/+ mutant mice have recapitulated the pathological features in eye and bone of human patients. Further analyses of the mutant phenotype with molecular markers will provide insight on how MAB21L2 guides the optic differentiation and skeletogenesis, revealing specific underlying pathogenic mechanism of the MAB21L2(R51C) mutation.
Forecasting private consumption with Google Trends data
Authors: Woo Jaemin , Owen Ann L. 
Published: October 2018

AbstractThis paper examines the predictive relationship of consumption‐related and news‐related Google Trends data to changes in private consumption in the USA. The results suggest that (1) Google Trends‐augmented models provide additional information about consumption over and above survey‐based consumer sentiment indicators, (2) consumption‐related Google Trends data provide information about pre‐consumption research trends, (3) news‐related Google Trends data provide information about changes in durable goods consumption, and (4) the combination of news and consumption‐related data significantly improves forecasting models. We demonstrate that applying these insights improves forecasts of private consumption growth over forecasts that do not utilize Google Trends data and over forecasts that use Google Trends data, but do not take into account the specific ways in which it informs forecasts.
Loss of TP53I11 Enhances the Extracellular Matrix‐independent Survival by Promoting Activation of AMPK
Authors: Xiao Tongqian , Xu Zhongjuan , Zhou Yuanshuai , Zhang Hai , Geng Junsa , Liang Yu , Qiao Hong , Suo Guangli 
Published: October 2018

AbstractExtracellular matrix (ECM)‐independent survival is an essential prerequisite for tumor metastasis, and a hallmark of epithelial cancer stem cells and epithelial–mesenchymal transition (EMT). Here, we found that loss of TP53I11 enhanced, and overexpression of TP53I11 suppressed the ECM‐independent survival, EMT, and migration in MCF10A cells. TP53I11 has long been considered as a transcriptional target of TP53. However, we found that TP53I11 regulated the ECM‐independent survival by a TP53‐independent way. As a metabolic sensor, AMPK promoted anoikis resistance by inhibiting AKT/m‐TOR/p70S6K signaling pathway. It was recently revealed that the reciprocal inhibitory relationship between AKT and AMPK regulated adaptation of cells to ECM‐detachment. Our results demonstrated that loss of TP53I11 promoted the activation of AKT/m‐TOR pathway, increased PGC‐1α expression and thereby enhanced OXPHOS in attach‐cultured MCF10A cells, but promoted AMPK activation to inhibit AKT/m‐TOR/p70S6K signaling pathway in detach‐cultured MCF10A cells. This indicates that TP53I11 functions as a mediator to balance activation of AKT and AMPK to adapt cells to different cellular contexts such as ECM‐attachment and ‐detachment. © 2018 IUBMB Life, 71(1):183–191, 2019
Sulphur contamination impact on seasonal and surface water chemistry on a reforested area of a former sulphur mine
Authors: Likus‐Cieślik Justyna , Smoliński Adam , Pietrzykowski Marcin , Bąk Andrzej 
Published: October 2018

AbstractThis work presents an assessment of seasonal changes and a chemometric study of surface water parameters on reclaimed and reforested areas of a former sulphur mine where the mineral was extracted using the Frasch method. Water samples were collected four‐times at 15‐monitored sampling points located on streams and ponds. Base chemical parameters of water (such as pH, EC, ion concentrations, and total P, S, and Al) were determined in laboratory conditions. A chemometric study with the application of the principal component analysis PCA method using the EM (expectation‐maximisation algorithm) procedure was employed to determine seasonal changes and similarities or dissimilarities between the water samples. The obtained results indicate an overall low seasonal and spatial variability of the investigated surface water parameters: mean pH for seasons from 6.2 to 6.7, EC 1,819–1,990 μS cm−1, S 380.7–484.2 mg L−1. However, the occurrence of hot spots was found (in three out of 15 monitoring points), which typically displayed very low pH (below 4.0), high EC (above 2,000 μS cm−1) and sulphur content (above 400 mg L−1), or a high proportion of calcium ions associated with the neutralisation of sulphurous soils. Trace elements occur in very low concentrations because most of the sampling points displayed high pH. The research was carried out in a unique location where high‐sulphur concentration in soils was not correlated with acidification but rather with alkalisation (hotspots). This phenomenon was related to the specificity of reclamation where post‐flotation lime were used.
Wind and trophic status explain within and among‐lake variability of algal biomass
Authors: Rusak J. A. , Tanentzap A. J. , Klug J. L. , Rose K. C. , Hendricks S. P. , Jennings E. , Laas A. , Pierson D. , Ryder E. , Smyth R. L. , White D. S. , Winslow L. A. , Adrian R. , Arvola L. , de Eyto E. , Feuchtmayr H. , Honti M. , Istvánovics V. , Jones I. D. , McBride C. G. , Schmidt S. R. , Seekell D. , Staehr P. A. , Zhu G. 
Published: October 2018

AbstractPhytoplankton biomass and production regulates key aspects of freshwater ecosystems yet its variability and subsequent predictability is poorly understood. We estimated within‐lake variation in biomass using high‐frequency chlorophyll fluorescence data from 18 globally distributed lakes. We tested how variation in fluorescence at monthly, daily, and hourly scales was related to high‐frequency variability of wind, water temperature, and radiation within lakes as well as productivity and physical attributes among lakes. Within lakes, monthly variation dominated, but combined daily and hourly variation were equivalent to that expressed monthly. Among lakes, biomass variability increased with trophic status while, within‐lake biomass variation increased with increasing variability in wind speed. Our results highlight the benefits of high‐frequency chlorophyll monitoring and suggest that predicted changes associated with climate, as well as ongoing cultural eutrophication, are likely to substantially increase the temporal variability of algal biomass and thus the predictability of the services it provides.
Defining Palliative Care Needs in Parkinson's Disease
Authors: Kluger Benzi M. , Shattuck Jo , Berk Julie , Sebring Kelly , Jones Wallace , Brunetti Fabian , Fairmont Isabel , Bowles Daniel W. , Sillau Stefan , Bekelman David B. 
Published: October 2018

AbstractObjectivesPalliative care addresses the suffering of patients and families affected by progressive illness through the management of medical symptoms, psychosocial issues, and spiritual concerns. Although there is an emerging interest in applying palliative care to Parkinson's disease (PD), potential palliative care needs have not been systematically investigated in PD patients. Our primary objective was to determine the prevalence of clinically significant symptomatic, psychosocial, and spiritual issues in PD and understand their impact on health‐related quality of life (HRQOL). Secondary objectives included comparing the level of palliative care needs of PD patients to advanced cancer patients and assessing preferences for advance care planning.MethodsNinety PD patients and 47 patients with advanced cancer were surveyed regarding potential palliative care needs, including symptom burden, mood, anticipatory grief, and spiritual well‐being. PD patients completed additional scales regarding HRQOL, motor symptoms, cognitive impairment, and preferences regarding advance care planning.ResultsPotential palliative care needs, including high symptom burden and grief, were common in PD patients and contributed to HRQOL even when controlling for depression and motor severity. In all domains investigated, PD patients had similar or higher levels of palliative care needs as patients with advanced cancer. PD patients expressed a desire to complete advance directives early in the disease course and with a physician.ConclusionsPalliative care needs contribute to HRQOL in PD and are of similar severity as cancer patients. This study supports and helps focus efforts to integrate palliative care principles in PD care across the spectrum of the disease.
Does prenylation predict progression in NAFLD?
Authors: Ghorbani Peyman , Smith Tyler KT , Fullerton Morgan D 
Published: October 2018

AbstractNon‐alcoholic fatty liver disease (NAFLD) often develops in concert with related metabolic diseases, such as obesity, dyslipidemia and insulin resistance. Prolonged lipid accumulation and inflammation can progress to non‐alcoholic steatohepatitis (NASH). Although factors associated with the development of NAFLD are known, triggers for the progression of NAFLD to NASH are poorly understood. Recent findings published in The Journal of Pathology reveal the possible regulation of NASH progression by metabolites of the mevalonate pathway. Mevalonate can be converted into the isoprenoids farnesyldiphosphate (FPP) and geranylgeranyl diphosphate (GGPP). GGPP synthase (GGPPS), the enzyme that converts FPP to GGPP, is dysregulated in humans and mice during NASH. Both FPP and GGPP can be conjugated to proteins through prenylation, modifying protein function and localization. Deletion or knockdown of GGPPS favors FPP prenylation (farnesylation) and augments the function of liver kinase B1, an upstream kinase of AMP‐activated protein kinase (AMPK). Despite increased AMPK activation, livers in Ggpps‐deficient mice on a high‐fat diet poorly oxidize lipids due to mitochondrial dysfunction. Although work from Liu et al provides evidence as to the potential importance of the prenylation portion of the mevalonate pathway during NAFLD, future studies are necessary to fully grasp any therapeutic or diagnostic potential. Copyright © 2018 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Benzothiazole‐Based‐Bioconjugates with Improved Antimicrobial, Anticancer and Antioxidant Potential
Authors: Kumari Bhawana , Chauhan Kalpana , Trivedi Jalpa , Jaiswal Varun , Kanwar Shamsher S. , Pokharel Yuba Raj 
Published: October 2018

AbstractMicro‐organism resistance to the drugs has made it necessary to explore novel antibacterial drugs that have significant toxicities. Herein, we report the bioconjugates of N‐acetyl‐D‐glucosamine with benzothiazole derivatives via ester linkage, which display potent effect against pathogenic micro‐organisms (E. coli, S. aureus and C. albicans). These newly synthesized compounds have also been evaluated for anticancer (Hep‐2 and caco cell lines) and antioxidant activities. The results supported that bioconjugates suppress pathogenic infections (in‐vitro) more effectively in comparison to equivalent dosage of non‐conjugated benzothiazole. Specifically, bioconjugates of Schiff derivative of methoxy‐benzothiazol (compound 8) and nitro‐ benzothiazole (compound 9) showed MIC value of 1.25 μg/mL against C. albicans. The above proved potent compound 9, furthermore, revealed significant activity against Hep‐2 and caco cell lines. In conclusion, this study proved that integration of biocomponent enhances the competence in biological application and the results can be explored as a lead for further improvization for commercialization.
Advanced Bio‐Based UV‐Curable Anticorrosive Coatings Reinforced by hBN
Authors: Zhao Hongran , Ding Jiheng , Yu Haibin 
Published: October 2018

AbstractHere, furfuryl methacrylate (FAM) was first successfully synthesized. Bulk hexagonal boron nitride (hBN) nanosheets were exfoliated and dispersed in FAM based on the strong π‐π interaction between hBN and FAM. The few‐layer hBN nanosheets as‐prepared possessed an average thickness of ∼3 nm (less than 10 layers) and exhibited superior storage stabilization under environment condition in FAM. Moreover, we creatively applied FAM solutions containing 0.25‐0.75 wt% of hBN as reactive diluents for the epoxidized soybean oil methacrylate (ESOM) matrix to effectively enhance its corrosion protection by suppressing the penetration of aggressive species. The electrochemical results revealed that the impedance of the coating containing 0.75 wt. % hBN is ∼200 times bigger than that of the blank coating specimen.
A data decomposition method for stepwise migration of complex legacy data
Authors: Martens Andreas , Book Matthias , Gruhn Volker 
Published: October 2018

SummarySooner or later, in almost every company, the maintenance and further development of large enterprise information technology (IT) applications reaches its limit. From the point of view of cost as well as technical capability, legacy applications must eventually be replaced by new enterprise IT applications. Data migration is an inevitable part of making this switch. While different data migration strategies can be applied, incremental data migration is one of the most popular strategies, due to its low level of risk: the entire data volume is split into several data tranches, which are then migrated in individual migration steps. The key to a successful migration is the strategy for decomposing the data into suitable tranches. This paper presents a method for data decomposition where the entire data volume of a monolithic enterprise IT application is split into independent data migration tranches. Each tranche comprises the data to be migrated in one migration step, which is usually executed during the application's downtime window. Unlike other methods, which describe data migration in a highly abstract way, we propose specific heuristics for data decomposition into independent data packages (tranches). The data migration approach described here is being applied in one of the largest migration projects currently underway in the European health care sector, comprising millions of customer records.