bluuebunny/crossref_metadata_2025_split huggingface dataset:
Challenges and opportunities of Italian-qualified pharmacists relocated to work in Great Britain: A cross-sectional survey
Authors: Pasculli Giuseppe , Giua Corrado , Keber Enrico , Cancellu Osvaldo , Arakawa Naoko , Manfrin Andrea 
Published: November 2024

Background: The Italian university system is actively revising the Master of Pharmacy (MPharm) curriculum to reflect post-COVID healthcare needs and a greater clinical focus. Understanding the challenges and opportunities faced by Italian pharmacists that relocated to Great Britain (GB) might provide valuable insights regarding the transferability of knowledge and skills obtained in the Italian pharmacy degree.
Aim: The study aimed to capture the perceptions of Italian pharmacists practising in GB regarding the Italian pharmacy degree programme and to explore the challenges and opportunities they face in their new professional environment, ultimately to inform the Italian government and related stakeholders (Ministry of University and Research, MUR) about the need to update the Italian MPharm curriculum to make it more clinically relevant and aligned with international standards.
Materials and methods: The authors developed a 50-item survey, pilot tested and reviewed for face and content validity by an expert panel. Participants’ characteristics, reasons for moving to GB, and data on their experience of practising as a pharmacist in Italy and GB were collected using Likert-type items and open- and closed-ended questions. Convenience sampling was used, and a sample power calculation was not deemed necessary. Statistical analysis involved descriptive analysis presenting frequencies and percentages for Likert-type and categorical items.
Results: An estimated 281 pharmacists qualified in Italy are registered with the General Pharmaceutical Council in GB. Of these, 54 took part in the survey, resulting in a probable coverage of the sample population of 19.2%. Respondents pointed to the lack of job satisfaction and limited future perspectives as the main reasons to leave Italy (38.9% and 83.3%, respectively). Other reasons to relocate to GB were clinically focused job opportunities and better salaries (68.5% and 79.6%, respectively). Notably, almost two-thirds of the participants affirmed that Italian pharmacy degrees were insufficient to meet the requirements for practising as a pharmacist in GB.
Conclusion: Different approaches to teaching clinical pharmacy and practical aspects seen in the pharmacy degree programmes in Italy and GB highlight a clear need for aligning the Italian pharmacy curriculum with the modern requirements of the pharmacy profession. Additionally, this alignment would facilitate the movement of Italian pharmacists into the GB healthcare system and beyond.
La période oratoire dans les remontrances parlementaires (entre la fin du xvie et le début du xviiie siècle)
Authors: Hache Sophie 
Published: November 2024

Quelles formes les périodes empruntent-elles dans les discours français, avec quels usages et quels effets ? L’étude d’une catégorie particulière de discours permet de nourrir cette réflexion : les remontrances parlementaires, qui constituent un genre oratoire institutionnel dont la forme est codifiée de la Renaissance à la Révolution, et qui relèvent du style élevé traditionnellement associé aux périodes. En s’appuyant sur deux recueils publiés à un siècle d’intervalle, le Recueil des remonstrances et actions publiques, faictes en la cour de Parlement et les Remontrances faites aux ouvertures de la Saint Martin de la Cour Souveraine de Lorraine et Barrois, l’article s’intéresse aux caractères qui font de la période un modèle : sa fréquence d’emploi reste stable, tandis que ses formes deviennent plus régulières et repérables, sur le plan syntaxique, mais aussi rythmique – conformément aux prescriptions des traités d’éloquence.
Fasting Plasma Glucose Levels in Relation to the Nutritional Status, Dietary Intake, and Lifestyle Factors among a Group of Sri Lankan Undergraduates: A Cross-Sectional Study
Authors: Kaldera P.H. , Wickramatilake C.M. 
Published: November 2024

Introduction: Diabetes mellitus (DM) is a global public health concern intricately linked to dietary habits, physical inactivity, and other lifestyle factors.
Objectives: This cross-sectional study aimed to elucidate the associations between fasting plasma glucose (FPG) levels, nutritional status, dietary patterns, and lifestyle factors among undergraduates at the Faculty of Allied Health Sciences, University of Ruhuna, Sri Lanka.
Materials & Methods: It was a descriptive cross-sectional study. Data were collected via a self-administered questionnaire. Anthropometric measurements were obtained following standard protocols. Fasting plasma glucose was quantified using the GOD- PAP method. Statistical analysis was performed using appropriate statistical tools.
Results: Participants (n = 100) were in the age range of 20 - 28 years and 58% were females. Their FPG levels ranged from 70 to 108 mg/dL with 3% of impaired fasting glycaemia (IFG) and no instances of DM. Mean (SD) BMI was 20.9 (3.4) kg/m2. Among them, 29% were overweight or obese. A statistically significant positive correlation was observed between FPG and both waist circumference (p=0.004) and BMI (p=0.019). Dietary assessment revealed suboptimal adherence to recommended portion sizes across all six food categories. Despite 82% consuming three main meals per day, meal-skipping was reported by 36% of the participants. Adequate water intake was reported among 60%, the majority abstained from alcohol and smoking. However, a considerable percentage reported consumption of junk foods. Physical inactivity and inadequate sleep were reported by 73% and 72% of the participants respectively.
Conclusions: The prevalence of IFG among the studied undergraduate population is low. However, the majority exhibited suboptimal dietary practices and insufficient engagement in physical activities, which warrant further attention for preventive health strategies.
Yes-associated protein-mediated melatonin regulates the function of periodontal ligament stem cells under oxidative stress conditions
Authors: Gu Ke , Feng Xiao-Mei , Sun Shao-Qing , Hao Xing-Yao , Wen Yong 
Published: November 2024

BACKGROUND
Human periodontal ligament stem cells (PDLSCs) regenerate oral tissue. In vitro expansion causes replicative senescence in stem cells. This causes intracellular reactive oxygen species (ROS) accumulation, which can impair stem cell function. Tissue engineering efficiency is reduced by exogenous ROS stimulation, which causes premature senescence under oxidative stress. Melatonin (MT), a powerful free radical scavenger, can delay PDLSCs senescence but may not maintain stemness under oxidative stress. This experiment examined the effects of hydrogen peroxide-induced oxidative stress on PDLSCs’ apoptosis, senescence, and stemness.
AIM
To determine if MT can reverse the above effects along with the underlying molecular mechanisms involved.
METHODS
PDLSCs were isolated from human premolars and cultured in different conditions. Flow cytometry was used to characterize the cell surface markers of PDLSCs. Hydrogen peroxide was used to induce oxidative stress in PDLSCs. Cell cycle, proliferation, apoptosis, differentiation, ROS, and senescence-associated β-galactosidase activity were assessed by various assays. Reverse transcription-polymerase chain reaction and western blot were used to measure the expression of genes and proteins related to stemness and senescence.
RESULTS
MT increases Yes-associated protein expression and maintains cell stemness in an induced inflammatory microenvironment, which may explain its therapeutic effects. We examined how MT affects PDLSCs aging and stemness and its biological mechanisms.
CONCLUSION
Our study reveals MT’s role in regulating oxidative stress in PDLSCs and Yes-associated protein-mediated activity, providing insights into cellular functions and new therapeutic targets for tissue regeneration.
Efficacy of serum-free cultured human umbilical cord mesenchymal stem cells in the treatment of knee osteoarthritis in mice
Authors: Xiao Kai-Zhen , Liao Gui , Huang Guang-Yu , Huang Yun-Long , Gu Rong-He 
Published: November 2024

BACKGROUND
We investigated the efficacy of intra-articular injection of human umbilical cord mesenchymal stem cells (hUC-MSCs) for the treatment of osteoarthritis (OA) progression in the knee joint. Although many experimental studies of hUC-MSCs have been published, these studies have mainly used fetal bovine serum-containing cultures of hUC-MSCs; serum-free cultures generally avoid the shortcomings of serum-containing cultures and are not subject to ethical limitations, have a wide range of prospects for clinical application, and provide a basis or animal experimentation for clinical experiments.
AIM
To study the therapeutic effects of serum-free hUC-MSCs (N-hUCMSCs) in a mouse model of knee OA.
METHODS
Fifty-five male C57BL/6 mice were randomly divided into six groups: The blank control group, model control group, serum-containing hUC-MSCs (S-hUCMSC) group, N-hUCMSC group and hyaluronic acid (HA) group. After 9 weeks of modeling, the serum levels of interleukin (IL)-1β and IL-1 were determined. Hematoxylin-eosin staining was used to observe the cartilage tissue, and the Mankin score was determined. Immunohistochemistry and western blotting were used to determine the expression of collagen type II, matrix metalloproteinase (MMP)-1 and MMP-13.
RESULTS
The Mankin score and serum IL-1 and IL-1β and cartilage tissue MMP-1 and MMP-13 expression were significantly greater in the experimental group than in the blank control group (P < 0.05). Collagen II expression in the experimental group was significantly lower than that in the blank control group (P < 0.05). The Mankin score and serum IL-1 and IL-1β and cartilage tissue MMP-1 and MMP-13 levels the experimental group were lower than those in the model control group (P < 0.05). Collagen II expression in the experimental group was significantly greater than that in the model control group (P < 0.05).
CONCLUSION
N-hUCMSC treatment significantly alleviate the pathological damage caused by OA. The treatment effects of the S- hUCMSC group and HA group were similar.
Exosome-based strategy against colon cancer using small interfering RNA-loaded vesicles targeting soluble a proliferation-inducing ligand
Authors: Kim Hyung-Jin , Lee Do Sang , Park Jung Hyun , Hong Ha-Eun , Choi Ho Joong , Kim Ok-Hee , Kim Say-June 
Published: November 2024

BACKGROUND
Recent advancements in nanomedicine have highlighted the potential of exosome (Ex)-based therapies, utilizing naturally derived nanoparticles, as a novel approach to targeted cancer treatment.
AIM
To explore the targetability and anticancer effectiveness of small interfering peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 RNA (siPIN1)-loaded soluble a proliferation-inducing ligand (sAPRIL)-targeted Exs (designated as tEx[p]) in the treatment of colon cancer models.
METHODS
tEx was generated by harvesting conditioned media from adipose-derived stem cells that had undergone transformation using pDisplay vectors encoding sAPRIL-binding peptide sequences. Subsequently, tEx[p] were created by incorporating PIN1 siRNA into the tEx using the Exofect kit. The therapeutic efficacy of these Exs was evaluated using both in vitro and in vivo models of colon cancer.
RESULTS
The tEx[p] group exhibited superior anticancer effects in comparison to other groups, including tEx, Ex[p], and Ex, demonstrated by the smallest tumor size, the slowest tumor growth rate, and the lightest weight of the excised tumors observed in the tEx[p] group (P < 0.05). Moreover, analyses of the excised tumor tissues, using western blot analysis and immunohistochemical staining, revealed that tEx[p] treatment resulted in the highest increase in E-cadherin expression and the most significant reduction in the mesenchymal markers Vimentin and Snail (P < 0.05), suggesting a more effective inhibition of epithelial-mesenchymal transition tEx[p], likely due to the enhanced delivery of siPIN1.
CONCLUSION
The use of bioengineered Exs targeting sAPRIL and containing siPIN1 demonstrated superior efficacy in inhibiting tumor growth and epithelial-mesenchymal transition, highlighting their potential as a therapeutic strategy for colon cancer.
Evaluation of Antibacterial and Antibiofilm Effects from Soil Streptomyces spp. against Multidrug-Resistant Bacteria
Authors: Prastya Muhammad Eka , Simbolon Sumihartati , Priyanto Jepri  Agung , Hasidu La Ode Abdul Fajar , Permatasari Vera , Primahana Gian , Dewi Rizna Triana , Kristiana Rhesi , Suryanti Erma 
Published: November 2024

The global increase in multidrug-resistant (MDR) bacterial infection has rapidly gained concern globally. This study aimed to investigate antibacterial and antibiofilm potential of 25 soil actinomycete strains against MDR strains including Escherichia coli strain M4, Pseudomonas aeruginosa strain M19, Klebsiella pneumoniae strain M19, Bacillus subtilis strain M18, and Methicillin Resistant Staphylococcus aureus (MRSA). In this study, three actinomycete isolates encoded APM-7, APM-11, and APM-21 showed a strong and broad antibacterial spectrum. The minimum inhibitory concentration (MIC) of extracts derived from these isolates was ranged from 78 μg/ml to 10,000 μg/ml. In addition, The extracts also displayed significant biofilm inhibition values ranging from 6.06 to 72.4%. Based on the results, APM-21 extract had the best antibacterial and antibiofilm activities with the strongest values against MRSA. According to the nucleotide sequencing of the 16S rRNA gene, APM-7, APM-11, and APM-21 strains possessed similar identities with Streptomyces cyaneus, Streptomyces coerulescens, and Streptomyces panayensis, respectively. Based on Liquid Chromatography Tandem-Mass Spectrometry (LC-MS/MS) analysis, two antibacterial compounds, namely rancimanycin III, and enteromycin were detected in all those three extracts. Interestingly, APM-21 extract also contained two prominent antibacterial substances including paramagnetoquinone C, and caerulomycin I, suggesting their contribution to the most potential activities. Moreover, new insights were provided into a promising candidate for use in an active compound combating strategy to control MDR bacterial strain infection.
Evaluation of Hursitism for Polycystic Ovary Syndrome Women At Al- Ramadi City
Authors: A.R. Alkubaisi , Aftan Al hayani Dhyauldeen , Subhi Noori Saif , Riyadh Abdul Kareem Al-Hetty Hussein 
Published: November 2024

The hyper-androgen and huritism in face and body in women is important diseases. It can be used as a possible marker in patients with ovary polycystic syndrome (PCOS). This study was carried out to evaluate DHEA and Testosterone levels associated with huritism and PCOS was studied in serum samples from pregnant, and non-pregnant patients and controls attending clinic Specialized. The study included 38 samples divided into three groups: 21 patients subjects, 6 pregnant women, 15 non-pregnant women with PCOS syndrome, and 17 normal subjects as control of the same age group. The target was selecting pregnant women due to changes in hormonal levels and physiological changes, as well as comparing them with non-pregnant women and control samples. The level of DHEAS was considerably higher in the median among pregnant women than in the control group and between non-pregnant and control groups without statistically significant differences between pregnant and non-pregnant values. Similarly, testosterone shows a statistically significant higher median among pregnant than control and also between non-pregnant and control groups without statistically significant difference between pregnant and non-pregnant values. In conclusion, this study detected a significant increase in testosterone and DHEA in non-pregnant women and then followed by pregnant women compared to the control, and age and the rise of these hormones are factors that cause the appearance of thick hair and thus cause polycystic ovary, which may lead to infertility.
Independent prognostic value of lipocalin-2 in congenital heart disease-associated pulmonary artery hypertension
Authors: Guo Zhang-Ke , Chen Ping-Gui , Li Yao-Xuan , Jiao Hong , Kong Xiao-Hui , Bai Song , Li Xiao-Feng , Liu Ai-Jun , Wang Guo-Liang 
Published: November 2024

BACKGROUND
Timely and accurate evaluation of the patient's pulmonary arterial pressure (PAP) is of great significance for the treatment of congenital heart disease. Currently, there is no non-invasive gold standard method for evaluating PAP.
AIM
To assess the prognostic value of lipocalin-2 (LCN2) in relation to PAP in patients with congenital heart disease associated with pulmonary artery hypertension.
METHODS
We conducted a retrospective analysis of 69 pediatric patients diagnosed with ventricular septal defects. The patients’ clinical and laboratory data were collected. The serum LCN2 concentrations were compared between the pulmonary arterial hypertension (PAH) group and the nonPAH group. The correlation of LCN2 concentration with PAH classification was evaluated using binary logistic regression analysis. The receiver operating characteristic (ROC) curve was used to evaluate the diagnostic potential of LCN2 for PAH.
RESULTS
Serum LCN2 concentration significantly correlated with patients’ mean PAP (r = 0.544, P < 0.001), but not correlated with creatinine (P = 0.446) or blood urea nitrogen (P = 0.747). LCN2 levels were significantly correlated with PAH in both univariate [odds ratio (OR) 1.107, 95%CI: 1.033-1.185, P = 0.004)] and multivariate regression analysis (OR 1.150, 95%CI: 1.027-1.288, P = 0.015). ROC curve analysis revealed an area under the curve of 0.783 for LCN2. At the cutoff value of 19.42 ng/mL, the sensitivity and specificity of LCN2 for diagnosing PAH is 90.19% and 55.56%, respectively. LCN2 concentration also significantly correlated with the post-repair mean PAP in patients with congenital heart disease (r = 0.532, P = 0.009).
CONCLUSION
LCN2 is emerging as a candidate biomarker for assessing PAP in patients with congenital heart disease. Its high sensitivity in diagnosing PAH makes it a valuable tool in patient management.
Role of a new inflammation predictor in predicting recurrence of atrial fibrillation after radiofrequency catheter ablation
Authors: Wang Yu-Jie , Liu Ke-Sen , Meng Xiang-Jiang , Han Xue-Fu , Nie Lu-Jing , Feng Wen-Jiu , Chen Yan-Bo 
Published: November 2024

BACKGROUND
Radiofrequency catheter ablation (RFCA) has become an important strategy for treating atrial fibrillation (AF), and postoperative recurrence represents a significant and actively discussed clinical concern. The recurrence after RFCA is considered closely related to inflammation. Systemic immune inflammation index (SII) is a novel inflammation predictor based on neutrophils, platelets, and lymphocytes, and is considered a biomarker that comprehensively reflects the immune inflammatory status of the body.
AIM
To explore the predictive effect of the SII on AF recurrence after RFCA and its predictive value in combination with the existing APPLE score for AF recurrence after RFCA in patients with non-valvular AF (NVAF).
METHODS
We retrospectively included 457 patients with NVAF first receiving RFCA and classified them into the recurrent or non-recurrent group. We also investigated the predictive role of SII on AF recurrence following RFCA. Finally, we explored and compared the additional predictive value of the SII after combining with the APPLE score.
RESULTS
After 12 months of follow-up, 113 (24.7%) patients experienced recurrence. High SII has been demonstrated to be an independent predictor for postoperative AF recurrence. Receiver operating characteristic and decision curve analysis (DCA), as well as net reclassification improvement (NRI) and integrated discrimination improvement (IDI) results, showed that SII combined with the APPLE score had higher predictive efficiency than using the SII or APPLE score alone. The area under the curve of the combined model (0.662, 95% confidence interval: 0.602-0.722) significantly increased compared with that of the SII and APPLE scores alone (P < 0.001). The combined model resulted in an NRI of 29.6% and 34.1% and IDI of 4.9% and 3.5% in predicting AF recurrence compared with the SII and APPLE scores alone, respectively (all P < 0.001). The SII, APPLE score, and their combination demonstrated greater clinical utility than did the treat-all and treat-none strategies over the 20–80% risk threshold according to the DCA.
CONCLUSION
The SII was a predictor of recurrence after RFCA of AF. Moreover, the SII enhanced the predictability of the APPLE score for post-RFCA AF recurrence, providing valuable insights for physicians to optimise patient selection and develop personalised treatment plans.
ԱԼ-ԲԱԼԱԶՈՒՐԻԻ «ԱԶՆՎԱԿԱՆՆԵՐԻ ԾԱԳՈՒՄՆԱԲԱՆՈՒԹՅՈՒՆՆԵՐԸ» ԱՇԽԱՏՈՒԹՅԱՆ ԽԱԲԱՐՆԵՐԻ ՎԵՐԼՈՒԾՈՒԹՅՈՒՆՆ՝ ԸՍՏ ԱՐԺԵՔԱՅԻՆ ՀԱՄԱԿԱՐԳԻ
Authors: Էլոյան Ալիս 
Published: November 2024

Ծագումնաբանությունն Արևելքի և Արևմուտքի շատ ժողովուրդների ավան­դական մշակույթի կարևոր ոլորտներից է: Ծագումնաբանական գիտելիքը (nasab) հա­­տուկ նշա­նակություն է ունեցել արաբների համար ոչ միայն նախաիսլամական և իսլամական ժամանակաշրջանում, այլև այն լայն տարածում ունի նաև ժամանակակից արաբա­կան աշխարհում: Ծա­գումնաբանական երկերն արաբական ավանդական գրավոր մշակույթի հիմ­­նա­կան մասն են կազմում։ Եվ ուշագրավ է տեքստաբանական վերլուծության ենթարկել 9-րդ դարի հայտ­նի պատ­միչ Ահմադ իբն Յահյա իբն Ջաբիր ալ-Բալազուրիի` արաբամուսուլմանական ծագումնաբանական գիտության կա­րևո­րա­գույն երկի` «Գիրք ազնվականների ծագումնաբանության մասին» («Kitāb Ansāb al-Ashrāf») –խաբարները։ Սույն հոդվածում փորձ է արվում պատմահամեմատական, պատմավեր­լուծա­կան, տիպաբանական և համադրական մեթոդներով՝ վեր հանել խաբարների խորքային առնչությունները, ինչպես նաև բնութագրել դրանցում առկա իմաստային գերակայություններն ըստ հաջորդայ­նութ­յան՝ առաջարկելով սեփական դասակարգումը: Սույն հոդվածի նպատակն է լուսաբանել խաբարների իմաստային և արժեքային, մշակութային շերտերը, որը կօգնի խաբա­ների համա­կող­մանի և ամբողջական ընկալմանը պատմական համատեքստում։
ПРОБЛЕМАТИКА ФИНАНСОВОГО РАВНОВЕСИЯ ОРГАНИЗАЦИЙ В СФЕРЕ ОБРАЗОВАНИЯ: ЗАРУБЕЖНЫЙ ОПЫТ
Authors: Е.В. Кроер 
Published: November 2024

В статье проведен анализ проблематики финансового равновесия зарубежных образовательных организаций. Рассмотрены условия финансового равновесия образовательной организации, принципы, разработан алгоритм достижения финансового равновесия. Представлены стратегии покрытия финансового дефицита образовательной организации, включающие, в том числе, образовательные инициативы, способствующие достижению финансового равновесия образовательных организаций.
The article analyzes the problems of financial balance of foreign educational organizations. The conditions of financial balance of an educational organization, principles, and an algorithm for achieving financial balance are considered. Strategies for covering the financial deficit of an educational organization are presented, including, among other things, educational initiatives that contribute to achieving financial balance of educational organizations.