bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of hydroxyapatite nanoparticles and wedelolactone on osteoblastogenesis from bone marrow mesenchymal stem cells
Authors: Dong Peipei , Zhu Di , Deng Xue , Zhang Yanjie , Ma Jinhui , Sun Xiaoxin , Liu Yanqiu 
Published: October 2018

AbstractRegenerative medicine has a high demand for the development of scaffold materials combined with other osteogenic inducers to generate bioactive composite materials for bone replacement therapies. Previously, we reported that wedelolactone promoted osteoblastogenesis of bone marrow mesenchymal stem cells (BMSCs). In this study, the effect of hydroxyapatites (HAps), bone composite materials we prepared, and the combined effect of wedelolactone and HAps on osteoblastogenesis differentiation was first evaluated. Three kinds of HAps constructed by a rod‐like shape with particle size of 25 nm (HAp‐1), 37 nm (HAp‐2), and 33 nm (HAp‐3) did not affect BMSC survival, but induced activity of alkaline phosphatase(ALP), a marker enzyme for osteoblastogenesis. HAp‐1 treatment resulted in a more significant increase in the number of ALP staining‐positive BMSC, and maintained an extended time for the increased number of ALP staining‐positive BMSC. Moreover, HAp‐1 combined with wedelolactone induced a higher ALP activity for a longer time than HAp‐2 and HAp‐3, and also increased the bone mineralization level. Osteoblastogenesis‐related marker genes expression including osteorix, osteocalcin, and runx2 were increased after BMSC were treated with HAp‐1 for 6 days. Although three kinds of HAps treatment for 9 days increased osteorix mRNA expression, osteocalcin, and runx2 mRNA expression levels were upregulated only by HAp‐1. Similarly, only HAp‐1 enhanced wedelolactone‐induced osteocalcin, osteorix, and runx2 mRNA expression after 9 days treatment. Together, these results suggested that HAps with different sizes generated different effect on osteoblastogenesis. HAp‐1 combined with wedelolacone can exert an enhanced effect on osteoblastogenesis, which has potential for treating osteoporosis. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 145–153, 2019.
Synthesis, characterization, antioxidant, antidiabetic, anticholinergic, and antiepileptic properties of novel N‐substituted tetrahydropyrimidines based on phenylthiourea
Authors: Maharramova Gunel , Taslimi Parham , Sujayev Afsun , Farzaliyev Vagif , Durmaz Lokman , Gulçin İlhami 
Published: October 2018

AbstractIn the presence of trifluoroacetic acid, on the basis of three‐component condensation of phenylthiourea with its salicylaldehyde and methyl‐3‐oxobutanoate, an efficient method for the synthesis of 1‐(4‐(2‐hydroxyphenyl)‐6‐methyl‐1‐phenyl‐2‐thioxo‐1,2,3,4‐tetrahydropyrimidin‐5‐yl)ethanone (I) has been worked out. These novel N‐substituted tetrahydropyrimidines based on phenylthiourea showed good inhibitory action against acetylcholinesterase (AChE), α‐glycosidase, and human carbonic anhydrase (hCA) isoforms I and II. K
i values of AChE enzyme were in the range of 0.48 to 7.46 nM. The hCA I and II were effectively inhibited by the compounds, with
K
i values in the range of 502.44 to 923.11 nM for hCA I and 400.32 to 801.57 nM for hCA II, respectively. The antioxidant activity of the novel N‐substituted tetrahydropyrimidines based on phenylthiourea was investigated by using different in vitro antioxidant assays; including 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH·) radical scavenging, Cu
2+ and Fe
3+ reducing activities.
Common health problems in safety‐net primary care: Modeling the roles of trauma history and mental health
Authors: Williams Allison B. , Smith Erin R. , Trujillo Michael A. , Perrin Paul B. , Griffin Sarah , Rybarczyk Bruce 
Published: October 2018

AbstractObjectiveThis study described trauma exposure and investigated mediational effects of mental health on the relationships between trauma and pain, sleep, smoking, and general health.MethodParticipants were 210 low‐income primary care patients. The study used a crosssectional, self‐report survey design.ResultsEighty‐five percent of the sample reported adult trauma and 54% reported four or more childhood traumas. Moderate or higher depression and anxiety levels were present in 59% and 48% of participants, respectively. Structural equation model fit was good for sleep, pain, and general health, showing that trauma indirectly affected health variables via mental health.ConclusionsParticipants endorsed substantial adult and childhood trauma, which likely had cascading effects on mental health and common primary care presenting health issues.
The psychometric properties of the Collaborative Assessment and Management of Suicidality rating scale
Authors: Corona Christopher D. , Gutierrez Peter M. , Wagner Barry M. , Jobes David A. 
Published: October 2018

AbstractObjectiveThe CAMS Rating Scale (CRS) is an adherence measure for the Collaborative Assessment and Management of Suicidality (CAMS), a suicide‐specific clinical intervention. This study examined the ability of the CRS to assess adherence to CAMS.MethodsVideo‐recorded therapy sessions of clinicians delivering either CAMS or Enhanced Care‐As‐Usual (E‐CAU) were rated with the CRS. These ratings (N = 98) were used to evaluate criterion validity, internal consistency, and factor structure.ResultsCriterion validity and factor analyses did not support the organization of the CRS into its current subscales. Furthermore, the identified factor model and item‐level statistics revealed weak CRS items. Finally, internal consistency was higher among CAMS clinicians than among clinicians delivering E‐CAU.ConclusionThese results establish the CRS as a measure that can effectively assess the adherence to CAMS in its current form. Potential revisions to future iterations of the CRS are discussed.
Selective removal of tungstate anions from aqueous solutions by surface anion‐imprinted ceramic membranes
Authors: Dong Zhihui , Zeng Jianxian , Zhou Hu , Lv Chaoqiang , Ma Yichang , Zeng Jiehui 
Published: October 2018

AbstractBACKGROUNDIt is significantly important to develop new technologies and materials for removal of tungstate anions (W(VI)) from aqueous solutions. An ion imprinted membrane not only possesses all virtues of a separation membrane, but also has tailor‐made recognition sites. In this work, a novel surface anion‐imprinted ceramic membrane (IIP‐PEI/CM) was fabricated to remove W(VI) selectively from aqueous solutions.RESULTSFT‐IR, XPS, TGA, SEM, streaming potential, pore size distribution and water flux confirmed the successful fabrication of IIP‐PEI/CM. With increasing pH, binding capacities (Qe) of W(VI) onto IIP‐PEI/CM and non‐imprinted membrane (NIP‐PEI/CM) rapidly decreased. At pH = 2.0, Qe values of IIP‐PEI/CM and NIP‐PEI/CM were 139.4 and 47.9 mg 100 g−1, respectively. Binding equilibrium was achieved within 3 min, showing an ultra‐fast kinetic process. Compared with NIP‐PEI/CM, IIP‐PEI/CM had higher selectivity for W(VI) in the presence of Mo(VI) and Cr(VI) ions, and its selectivity coefficients for W(VI)/Mo(VI) and W(VI)/Cr(VI) were 15.47 and 19.74, respectively. During dynamic filtration, IIP‐PEI/CM was effective and selective for removal of W(VI), and it had a good reusability. Protonated amine groups within PEI participated the uptake of W(VI) by electrostatic interactions.CONCLUSIONIIP‐PEI/CM showed ultrafast and selective binding of W(VI) anions, and has potential application for W(VI) removal/recovery from aqueous solutions. © 2018 Society of Chemical Industry
The effects of endogenous and exogenous catecholamines on hypoxic cardiac performance in red‐bellied piranhas
Authors: Joyce William , Williams Catherine J. A. , Iversen Sofie , Henriksen Per Guldhammer , Bayley Mark , Wang Tobias 
Published: October 2018

AbstractCatecholamines protect the fish heart during hypoxia. However, the humoral adrenergic stress response may only be invoked in extremis. We investigated the hypothesis that endogenous (e.g., neuronal) myocardial catecholamines may also impact cardiac performance during hypoxia in a hypoxia‐tolerant tropical fish, the red‐bellied piranha (Pygocentrus nattereri). First, we measured endogenous tissue catecholamines and in vitro catecholamine release from piranha myocardium using ultraperformance liquid chromatography. Ventricle homogenates contained detectable levels of both adrenaline (7.27 ng/g) and noradrenaline (14.48 ng/g), but only noradrenaline was released from ventricular tissue incubated in Ringer’s solution. Noradrenaline released in this assay was not affected by hypoxia but was promoted by the catecholamine releasing agent tyramine. Our second series of experiments explored cardiac contractile performance in vitro using tyramine, exogenous noradrenaline or adrenaline, and propranolol (a β‐adrenoceptor antagonist). In ventricular strip preparations, β‐adrenergic blockade with propranolol had no effects on twitch force or contraction kinetics in either normoxia or hypoxia, confirming that spontaneous endogenous catecholamine release did not impact cardiac performance. However, in the absence of propranolol, tyramine mimicked the positive inotropic effect of noradrenaline (10 µM) during hypoxia, although adrenaline was capable of generating larger effects. Our results suggest that, although it is not spontaneously released, inducible endogenous noradrenaline release may have a significant β‐adrenoceptor‐dependent impact on hypoxic performance in the fish heart.
Light at night affects hippocampal and nidopallial cytoarchitecture: Implication for impairment of brain function in diurnal corvids
Authors: Taufique Sheikh Tahajjul , Prabhat Abhilash , Kumar Vinod 
Published: October 2018

AbstractOur previous studies have shown that light at night (LAN) impaired cognitive performance and affected neurogenesis and neurochemistry in the cognition‐associated brain regions, particularly the hippocampus (HP) and lateral caudal nidopallium (NCL) of Indian house crows (Corvus splendens). Here, we examined the cytoarchitecture and mapped out the morphology of neurons and glia–neuron density in HP and NCL regions of crows that were first entrained to 12‐hr light (LL): 12‐hr darkness (LD) and then exposed to the light regime in which 12‐hr darkness was either replaced by daytime light (i.e., constant light, LL) or by dim light (i.e., dim light at night, dLAN), with controls continued on LD 12:12. Compared with LD, there was a significant decrease in the soma size, suggesting reduced neuronal plasticity without affecting the neuronal density of both HP and NCL of crows under LL and dLAN conditions. In parallel, we found a reduced number of glia cells and, hence, decreased glia–neuron ratio positively correlated with soma size in both, HP and NCL regions. These results for the first time demonstrate LAN‐induced negative effects on the brain cytoarchitecture of a diurnal species and give insight for possible influence on the brain health and functions in animals including humans that might be inadvertently exposed to LAN in an emerging night‐illuminated urban environment.
Strong chromatic index of unit distance graphs
Authors: Dębski Michał 
Published: October 2018

AbstractThe strong chromatic index of a graph , denoted by , is defined as the least number of colors in a coloring of edges of , such that each color class is an induced matching (or: if edges and have the same color, then both vertices of are not adjacent to any vertex of ). A graph is a
unit distance graph in if vertices of can be uniquely identified with points in , so that is an edge of if and only if the Euclidean distance between the points identified with and is 1. We would like to find the largest possible value of , where is a unit distance graph (in and ) of maximum degree . We show that , where is a unit distance graph in of maximum degree . We also show that the maximum possible size of a strong clique in unit distance graph in is linear in and give a tighter result for unit distance graphs in the plane.
Improved, one‐pot synthesis of 6‐[18F]fluorodopamine and quality control testing for use in patients with neuroblastoma
Authors: Vāvere Amy L. , Neumann Kiel D. , Butch Elizabeth R. , Hu Bao , DiMagno Stephen G. , Snyder Scott E. 
Published: October 2018

6‐[18F]Fluorodopamine ([18F]F‐DA) is taken into cells via the norepinephrine transporter (NET). Recent [18F]F‐DA positron emission tomography–computed tomography (PET‐CT) imaging of adult neuroendocrine tumors shows a dramatic improvement in sensitivity over the standard‐of‐care, meta‐iodobenzylguanidine (MIBG) single‐photon emission computed tomography (SPECT)–CT. A new precursor (ALPdopamine™) allows no‐carrier‐added synthesis resulting in high–molar activity [18F]F‐DA. Automated synthesis of [18F]F‐DA was performed in a single reactor using a two‐step procedure: 1) fluorination via thermolysis of a diaryliodonium salt precursor, followed by 2) acid hydrolysis. Phase transfer agents, Kryptofix 222 and two tetraalkylammonium salts, were investigated. Optimized synthesis of [18F]F‐DA was achieved in 56 to 60 minutes (26% end of synthesis [EOS], nondecay corrected). The product passed all Food and Drug Administration (FDA)–required quality control testing for human use. Accumulation of [18F]F‐DA in SK‐N‐BE(2)‐C (high NET expression) cells was significantly higher than in SH‐EP (minimal NET expression) cells (P < 0.0001). ALPdopamine provides an effective scaffold for the routine production of [18F]F‐DA for human use. Validation of uptake by neuroblastoma (NB) cell lines supports the use of [18F]F‐DA for imaging NB patients. A pediatric NB imaging trial using [18F]F‐DA PET has been approved (Investigational New Drug application (IND) no. 138638) based on the methods reported here. We expect [18F]F‐DA will be localized in NB tumors and that high‐quality functional images will be obtained within minutes after injection.
Quantitative MRI of visually intact rotator cuff muscles by multiecho Dixon‐based fat quantification and diffusion tensor imaging
Authors: Kälin Pascal S. , Huber Florian A. , Hamie Qeumars M. , Issler Lydia S. , Farshad‐Amacker Nadja A. , Ulbrich Erika J. , Guggenberger Roman 
Published: October 2018

BackgroundQuantitative MRI allows assessment of shoulder rotator cuff (RC) muscles by Dixon MR sequences with calculation of fractional fat content (FF%) maps and diffusion tensor imaging (DTI) including tractography.PurposeTo compare FF% and DTI derived parameters among visually intact RC muscles, to compare 2D with 3D DTI measurements and to establish normative values.Study TypeProspective.SubjectsForty patients aged >18 years undergoing shoulder MR arthrography were included.Field Strength/SequenceMR arthrography of the shoulder including 3D multiecho Dixon and 3D echo‐planar DTI sequences (15 gradient encoding directions, b‐value 600 s/mm2) was performed at 3.0T.AssessmentMuscles affected by RC tears or fatty infiltration of Goutallier grade ≥1 were excluded. Two independent radiologists measured FF%, apparent diffusion coefficient (ADC), and fractional anisotropy (FA) by region‐of‐interest (ROI) placements at the Y‐position of the scapula and 3D tractography of each muscle with qualitative evaluation was performed.Statistical TestsIntraclass correlation coefficients (ICCs) and Cohen's kappa were used for interreader agreement and Pearson correlation coefficient to correlate quantitative measures with each other and age, independent‐samples t‐test, one‐way analysis of variance (ANOVA), and Kruskal–Wallis test were performed to investigate differences between genders and muscles.ResultsQualitative and quantitative measurements showed moderate (κ = 0.41–0.56) to almost perfect (ICC = 0.75–0.99) agreement. There were weak but significant positive correlations of FF% with age (r = 0.273, P < 0.05) and FA‐2D (r = 0.319–0.383, P < 0.05). Significant differences were found among RC muscles for ADC, radial diffusivity (RD), and tract homogeneity (all P < 0.05) but not between genders (all P ≥ 0.05). High correlations of 2D with 3D measurements for ADC (r = 0.639, P < 0.001) and FA (r = 0.628, P < 0.001) were seen.Data ConclusionQuantitative MRI with estimation of FF% and DTI parameters shows significant age‐associated changes and differences among visually intact RC muscles. High reproducibility and correlations of 2D with 3D DTI measurements can be expected.Level of Evidence: 2Technical Efficacy: Stage 2J. Magn. Reson. Imaging 2019;49:109–117.
Evaluation of Effects of TGF‐β1 Inhibition on Gastric Cancer in Nude Mice by Diffusion Kurtosis Imaging and In‐Line X‐ray Phase Contrast Imaging With Sequential Histology
Authors: Shi Bowen , Yuan Fei , Yan Fuhua , Zhang Huan , Pan Zilai , Chen Weibo , Wang Guilong , Tan Jingwen , Zhang Yang , Ren Yuqi , Du Lianjun 
Published: October 2018

BackgroundAccurate and complete response evaluation after treatment is important to implement individualized therapy for gastric cancer.PurposeTo investigate the effectiveness of diffusion kurtosis imaging (DKI) and in‐line X‐ray phase contrast imaging (ILXPCI) in the assessment of the therapeutic efficacy by transforming growth factor beta 1 (TGF‐β1) inhibition.Study TypeProspective animal study.Animal ModelThirty nude mice subcutaneous xenotransplantation tumor model of gastric cancer for DKI and 10 peritoneal metastasis nude mice model for ILXPCI.Field Strength/SequenceExaminations before and serially at 7, 14, 21, and 28 days after TGF‐β1 inhibition treatment were performed at 3T MRI including T2‐weighted imaging (T2WI) and DKI with five b values of 0, 500, 1000, 1500, 2000 s/mm2; ILXPCI examinations were performed at 14 days after treatment.AssessmentDKI parameters (apparent diffusion coefficient [ADC], diffusivity [D] and kurtosis [K]) were calculated by two experienced radiologists after postprocessing.Statistical TestsFor the differences in all the parameters between the baseline and each timepoint for both the treated and the control mice, the Mann–Whitney test was used. The Spearman correlation test was used to evaluate correlations among the DKI parameters and corresponding pathologic necrosis fraction (NF).ResultsADC, D, and K values were significantly different between the two groups after treatment (P < 0.05). Serial measurements in the treated group showed that the ADC, D, and K values were significantly different at 7, 14, 21, and 28 days compared with baseline (P < 0.05). There were significant correlations between DKI parameters and NF (ADC, r = 0.865, P < 0.001; D, r = 0.802, P < 0.001; K, r = –0.944, P < 0.001). The ILXPCI results in the treated group showed a stronger absorption area than the control group.Data ConclusionDKI may be used to evaluate the complete course therapeutic effects of gastric cancer induced by TGF‐β1 inhibition, and the ILXPCI technique will improve the tumor microstructure resolution.Level of Evidence: 1Technical Efficacy: Stage 4J. Magn. Reson. Imaging 2019;49:1553–1564.
Evaluation of Focal Laser Ablation of Prostate Cancer Using High Spectral and Spatial Resolution Imaging: A Pilot Study
Authors: Wang Shiyang , Fan Xiaobing , Yousuf Ambereen , Eggener Scott E. , Karczmar Gregory , Oto Aytekin 
Published: October 2018

BackgroundFocal laser ablation (FLA) is a minimally invasive thermal ablation, guided by MRI through an optical fiber, to induce coagulative necrosis in cancer.PurposeTo evaluate the feasibility of high spectral and spatial resolution imaging using multiecho gradient echo (MEGE) MRI for identification of ablation zones, after FLA of prostate cancers.Study TypeProspective.PopulationFourteen patients with biopsy‐confirmed localized prostate cancers.Field Strength/SequenceFLA was performed under monitored conscious sedation with a 1.5T MRI scanner. Axial MEGE images were acquired before and after the last FLA. Pre‐ and postcontrast enhanced T1‐weighted (pT1W) images were acquired to assess the FLA zone as a reference standard.AssessmentThe maps and water resonance peak height (WPH) images were calculated from the MEGE data. Ablation area was outlined using an active contour method. The maximum ablation area and total ablation volume were calculated from and WPH images, and compared with the sizes measured from pT1W images.Statistical TestsNonparametric Kruskal–Wallis tests were performed to determine whether there was significant difference in calculated ablation areas and volumes between , WPH, and pT1W images.ResultsAverage (38.9 ± 14.1 msec) in the ablation area was significantly shorter (P = 0.03) than the preablation area (57.8 ± 25.3 msec). The normalized WPH value over the ablation area (1.3 ± 0.6) was significantly decreased (P = 0.02) more than the preablation area (2.0 ± 0.9). The maximum ablation areas measured by (295.7 ± 96.4 mm2), WPH (312.2 ± 63.0 mm2), and pT1W (320.3 ± 82.9 mm2) images were all similar. Furthermore, there was no significant difference (P = 0.31) for measured ablation volumes 3310.5 ± 649.5, 3406.4 ± 684.9, and 3672.5 ± 832.4 mm3 between , WPH, and pT1W images, respectively.Data Conclusion and WPH images provide acceptable measurements of ablation zones during FLA treatment of prostate cancers without the need for contrast agent injection. This might allow repeated assessment following each heating period so that subsequent ablations can be optimized.Level of Evidence: 2Technical Efficacy: Stage 5J. Magn. Reson. Imaging 2019;49:1374–1380.
Epidemiology of human adenovirus infection in children hospitalized with lower respiratory tract infections in Hunan, China
Authors: Xie Leyun , Zhang Bing , Xiao Niguang , Zhang Fei , Zhao Xin , Liu Qin , Xie Zhiping , Gao Hanchun , Duan Zhaojun , Zhong Lili 
Published: October 2018

AbstractTo investigate the current genotypes of circulating human adenovirus (HAdV) strains, we molecularly genotyped HAdV in the nasopharyngeal aspirates (NPAs) of patients with acute lower respiratory tract infections (ALRTIs) and attempted to determine their associations with clinical symptoms. A total of 4751 NPA samples were collected from 4751 patients admitted to Hunan Provincial People's Hospital from September 2007 to March 2014, of which 447 (9.4%) samples were HAdV positive. Fourteen different HAdV types were identified; HAdV types 1 to 7 (HAdV 1‐7) were identified in 95.7% of the 447 NPA samples with HAdV‐7 and HAdV‐3 being the most prevalent. In addition, 93.3% (417 of 447) of patients were younger than 5 years. The incidence of HAdV infection peaked in summer. Different HAdV types showed a predilection for different age groups and different seasonal distribution patterns. Coinfection of HAdVs and other respiratory viruses was detected in 63.3% (283 of 447) of the HAdV‐positive samples. The most common clinical diagnosis was pneumonia and the most common symptoms were fever and cough. In comparison with children infected with HAdV‐3 alone, those infected with HAdV‐7 alone had an increased frequency of severe pneumonia involvement (11.6% vs 32.4%; P = 0.031), higher intensive care unit admission rates (7.0% vs 26.5%; P = 0.019), and a longer length of hospital stay (P = 0.03). Mixed infections in younger children were associated with a longer hospital stay (P = 0.023). Our results demonstrate the recent changes in the trends of circulating HAdV genotypes associated with ALRTIs in Hunan China.
The Horizon and History of Asia Pacific Heart Rhythm Society
Authors: Hiraoka Masayasu 
Published: October 2018

AbstractThe Asia Pacific Heart Rhythm Society (APHRS) has grown very rapidly and efficiently after its founding in 2008. The success of our organization and rapid growth was due to the enthusiastic support by pioneer electrophysiologists and efforts by many physicians to cure heart rhythm disorders. With the experience of preceding arrhythmia symposia of inter‐cities or international basis in the region, the organization could quickly grow to become APHRS. The mission of APHRS is to promote basic research and improve clinical practice, optimize care of patients with heart rhythm disorders, and to train and encourage young physicians to actively participate and promote the activity of our society.