bluuebunny/crossref_metadata_2025_split huggingface dataset:
Die PPmP im Spiegel der Zitierungen
Authors: Brähler Elmar , Tibubos Ana 
Published: December 2018

Unsere Zeitschrift erfüllt für ihre Leserinnen und Leser viele Funktionen. Sie soll der Fort- und Weiterbildung dienen, auch fachpolitische Übersichten geben, aber vor allem auch über den wissenschaftlichen Fortschritt und die Arbeit in den Fachgebieten Psychosomatik, Psychotherapie und Medizinische Psychologie informieren.Die wissenschaftliche Reputation einer Zeitschrift wird oft daran festgemacht, in welcher Weise Beiträge in anderen wissenschaftlichen Aufsätzen rezipiert werden. Das Jahresende legt es nahe, wieder einmal einen Blick auf die Rezeption der Zeitschrift zu werfen. Wir wollen zum einen eine Längsschnittbetrachtung machen in dem Sinne, welche Artikel der Zeitschrift langfristig und kontinuierlich häufig zitiert wurden. Später wollen wir auf die Rezeption der in jüngster Zeit erschienenen Artikel eingehen.In Tab. 1 sind die Artikel der Zeitschrift aufgeführt, die durchschnittlich mindestens fünfmal im Jahr in der wissenschaftlichen Literatur zitiert wurden.
Zirkulierende Tumorzellen, zirkulierende Tumor-DNA und zirkulierende microRNA beim metastasierten Mammakarzinom – oder: Welche Rolle spielt die Liquid Biopsy beim Brustkrebs?
Authors: Polasik Arkadius , Tzschaschel Marie , Schochter Fabienne , de Gregorio Ameli , Friedl Thomas , Rack Brigitte , Hartkopf Andreas , Fasching Peter , Schneeweiss Andreas , Müller Volkmar , Huober Jens , Janni Wolfgang , Fehm Tanja 
Published: December 2018

ZusammenfassungDie Streuung von Tumorzellen und Entstehung solider Metastasen findet sowohl über das Lymph- als auch das Blutsystem statt. Der Nachweis zirkulierender Tumorzellen (CTCs) und der zirkulierenden Tumor-DNA (ctDNA) im venösen Blut ist sowohl beim frühen als auch beim metastasierten Mammakarzinom möglich. Ihre prognostische Relevanz wurde bereits mehrfach bewiesen. Dabei ist die repetitive Untersuchung der CTCs bzw. ctDNA im Sinne einer regelmäßigen „liquid biopsy“ jederzeit und problemlos möglich. Durch die zusätzlichen molekularen Analysen ist es möglich, Tumorcharakteristika und ihre Heterogenität, die mit möglichen Resistenzen einhergehen, zu definieren. Dies ermöglicht den Einsatz einer personalisierten und zielgerichteten Therapie, um neben einem verlängerten Gesamtüberleben auch die Verbesserung der Lebensqualität zu erreichen.
Die Rolle von Biomarkern bei Diagnose, Prognose und Therapie von Patienten unter immunonkologischer Therapie mit urologischen Malignomen
Authors: Compérat Eva , Eymerit Caroline , Moroch Julien , Varinot Justin 
Published: December 2018

ZusammenfassungIn den letzten Jahren haben Fortschritte in der Grundlagenforschung neue Einsichten in die Tumorbiologie gestattet, dadurch konnten neue Therapien entwickelt werden. Diese Übersichtsarbeit beleuchtet die Rolle des Pathologen und wie er anhand von Gewebemarkern helfen kann, eine Therapieentscheidung zu treffen. Wir erklären die Problematik der molekularen Blasentumoruntergruppen, die Rolle der neoadjuvanten Chemotherapie und die der Immuncheckpoint-Inhibitoren; des Weiteren gehen wir auf das Nierenkarzinom und dessen Therapiemöglichkeiten ein, mit besonderem Augenmerk auf das klarzellige Nierenkarzinom als häufigsten Nierenkarzinomtyp. Wir streifen kurz das Prostatakarzinom, das als hormonabhängiger Tumor wahrscheinlich einen anderen Therapieansatz braucht. Wir versuchen, die Machbarkeit und Grenzen vom pathologischen Standpunkt her zu beleuchten.
Percutaneous Gastrostomy Placement for Decompression of the Excluded Gastric Remnant following Roux-en-Y Gastric Bypass Surgery
Authors: Majumdar Shamaita , Tiwari Tatulya , Akinwande Olaguoke , Ramaswamy Raja 
Published: December 2018

Abstract
Purpose To evaluate the feasibility and safety of percutaneous gastrostomy for decompression of the excluded stomach in patients’ status post Roux-en-Y gastric bypass (RYGB).
Materials and Methods Between January 2001 and August 2017, 10 consecutive RYGB patients who underwent placement of a decompressive gastrostomy of the excluded stomach were identified in an institutional database. Technical success was defined as successful gastrostomy catheter placement in the bypassed stomach using fluoroscopy and/or ultrasound guidance. Clinical success was established if dilation of the excluded stomach improved after gastrostomy with resolution of associated symptoms. Charts were reviewed for treatment-related adverse events post-procedure.
Results The cohort was predominantly female (9/10), with an average age of 54 ± 14 years. Median follow-up was 35.2 months (range: 0.6–115). Indications for decompressive gastrostomy placement included small bowel obstruction (6/10) or afferent limb obstruction at the jejunojejunal anastomosis (4/10). The most common presenting symptoms were abdominal pain, distension, and vomiting. All patients had successful gastrostomy placement in the excluded remnant, using ultrasound and fluoroscopic guidance, with no procedural complications. The 12 to 16F Cope loop catheters was used in this cohort, and gastropexy sutures were used in two cases. All 10 patients demonstrated clinical resolution of symptoms after gastrostomy placement. Two patients developed minor complications of tube site leakage and poor tube function requiring gastrostomy exchange within 1 week of the procedure.
Conclusion Fluoroscopic and ultrasound-guided percutaneous gastrostomy catheter placement is a safe, effective, and feasible approach to treating dilation of the excluded gastric remnant in RYGB patients.
Dried blood spot microbiological assay indicates high prevalence of folate deficiency in rural adult men and women in the Haitian Central Plateau
Authors: Harrell Shreckengost Constance , Kim Andrew W , Whitaker Sarah H , Weng Lei , Pearce Bradley D , Kaiser Bonnie N 
Published: December 2018

Abstract

Background
Although folate deficiency is linked to adverse health effects, limited data exist characterizing the problem in rural settings. This study determined the prevalence of folate deficiency and anemia in rural adults in the Haitian Central Plateau using combined laboratory methods.

Methods
Dried blood spots (DBSs) and hemoglobin measurements were collected from adult men and women selected by cluster random sampling in Haiti’s Central Plateau. DBSs were analyzed for folate using a microbiological assay. Hemoglobin levels were determined using both a HemoCue photometer and the sodium lauryl sulfate microplate method. Red cell folate (RCF) levels were determined by normalizing DBS folate to hemoglobin.

Results
Of the 197 subjects assessed for hemoglobin, 11.4% of males and 21.0% of females were anemic (male: hemoglobin<12 g/dL; female: hemoglobin<11 g/dL). Of the 173 subjects assessed for RCF, 27.9% of men and 14.9% of women were folate deficient (RCF<340 nmol/L). Among reproductive-age women, 83.6% had RCF levels associated with a risk of neural tube defects of >14 per 10 000 live births (RCF≤699 nmol/L).

Conclusions
Adults in the Haitian Central Plateau suffer from high rates of anemia and folate deficiency, putting the population at elevated risk for disease. DBSs and microbiological assay make folate evaluation feasible, even in low-resource regions.
Possible cold-adaptation for the fungal kinesin in compensation for thermal stability acquired by single amino acid substitution
Authors: Shimizu Youské , Togawa Toru , Chaen Shigeru 
Published: December 2018

AbstractThe amino acid sequence of the motor domain of AnKinA, kinesin-1 from Aspergillus nidulans, growing optimally at 37°C, was compared with that of SbKin1, kinesin-1 from the snow mold Sclerotinia borealis. For cold-adaptation, some enzymes are thought to exhibit augmented protein structure flexibility, acquired most effectively by substituting a glycine residue for another amino acid residue. By the comparison described above, two glycine residues proximal to tightly bound ADP were identified in the SbKin1 motor domain, of which the corresponding residues of AnKinA were non-glycine ones (P60 and S323). We made AnKinA recombinant kinesin (AnKinA-WT (WT)) along with P60G and S323G mutants. From the basal ATPase activity (without microtubules), these kinesins showed similar characteristics in activation energies, while deviation from the linearity of the ATPase activity time-course was detected at 34°C for WT and P60G but at 24°C for S323G. The microtubule translocation velocity of WT, P60G or S323G exhibited an activation energy of 60, 58 or 53 kJ/mol, respectively; for S323G, the activation energy was lower and the velocity at low temperatures was higher than those for the other two. These results suggest that the point mutation S323G would offer possible cold-adaptation in compensation for thermal stability.
Understanding memories of the Holocaust—A new approach to neural networks in the digital humanities
Authors: Blanke Tobias , Bryant Michael , Hedges Mark 
Published: December 2018

Abstract
This article addresses an important challenge in artificial intelligence research in the humanities, which has impeded progress with supervised methods. It introduces a novel method to creating test collections from smaller subsets. This method is based on what we will introduce as distant supervision’ and will allow us to improve computational modelling in the digital humanities by including new methods of supervised learning. Using recurrent neural networks, we generated a training corpus and were able to train a highly accurate model that qualitatively and quantitatively improved a baseline model. To demonstrate our new approach experimentally, we employ a real-life research question based on existing humanities collections. We use neural network based sentiment analysis to decode Holocaust memories and present a methodology to combine supervised and unsupervised sentiment analysis to analyse the oral history interviews of the United States Holocaust Memorial Museum. Finally, we employed three advanced methods of computational semantics. These helped us decipher the decisions by the neural network and understand, for instance, the complex sentiments around family memories in the testimonies.
The Colic of Madrid (1788–1814): Experts, Poisons, Politics, and War at the End of the Ancien Régime in Spain
Authors: Bertomeu-Sánchez José Ramón 
Published: December 2018

Summary
This paper explores the management of health hazards caused by the use of lead compounds in food vessels. My focus is on the regulations promulgated in 1801 in Spain. The main protagonists of the paper are late eighteenth-century members of the Madrid Academy of Medicine interested in public health issues, chemists with contrasting views on the regulation of toxic hazards, enlightened artisans with particular ways of knowing and doing, and French military surgeons involved in the long-term controversy concerning the causes and remedies of what was known as the ‘colic of Madrid’. Many general issues regarding the regulation of toxic risks are discussed: the disputed fields of expertise, the changing social anxieties, the diverse agency of the stakeholders and decision makers, the technologies for identifying poisons and for assessing their effects, and the persistent tensions and conflicts inside the various professional and academic groups.
Galectin-3, A Novel Endogenous Trem2 Ligand, Regulates Inflammatory Response and Aβ Fibrilation in Alzheimer’s Disease
Authors: Boza-Serrano Antonio , Ruiz Rocío , Sanchez-Varo Raquel , Yang Yiyi , García-Revilla Juan , Jimenez-Ferrer Itzia , Paulus Agnes , Wennström Malin , Vilalta Anna , Allendorf David , Davila Jose Carlos , Dunning Christopher , Stegmayr John , Jiménez Sebastian ,   The Netherland Brain, Roca-Ceballos Maria Angustias , Navarro-Garrido Victoria , Swanberg Maria , Hsieh Christine L. , Miguel-Real Luis , Englund Elisabet , Linse Sara , Leffler Hakon , Nilsson Ulf J. , Gutierrez Antonia , Brown Guy C. , Vitorica Javier , Venero Jose Luis , Deierborg Tomas 
Published: December 2018

ABSTRACTAlzheimer’s disease (AD) is a progressive neurodegenerative disease in which the formation of extracellular aggregates of amyloid beta (Aβ) peptide, intraneuronal tau neurofibrillary tangles and microglial activation are major pathological hallmarks. One of the key molecules involved in microglial activation is galectin-3 (gal3), and we demonstrate here for the first time a key role of gal3 in AD pathology. Gal3 was highly upregulated in the brains of AD patients and 5xFAD (familial Alzheimer’s disease) mice, and found specifically expressed in microglia associated with Aβ plaques. Single nucleotide polymorphisms in the LGALS3 gene, which encodes gal3, were associated to an increased risk of AD. Gal3 deletion in 5xFAD mice attenuated microglia-associated immune responses, particularly those associated with TLR and TREM2/DAP12 signaling. In vitro data demonstrated the requirement of gal3 to fully activate microglia in response to fibrillar Aβ. Gal3 deletion decreased the Aβ burden in 5xFAD mice and improved cognitive behavior. Electron microscopy of gal3 in AD mice demonstrated i) a preferential expression of gal3 by plaque-associated microglia, ii) its presence in the extracellular space and iii) its association to Aβ plaques. Low concentrations (1 nM) of pure gal3 promoted cross-seeding fibrilization of pure Aβ. Importantly, a single intrahippocampal injection of gal3 along with Aβ monomers in WT mice was sufficient to induce the formation of insoluble Aβ aggregates that were absent when gal3 was lacking. High-resolution microscopy (STORM) demonstrated close co-localization of gal3 and TREM2 in microglial processes, and a direct interaction was shown by a fluorescence anisotropy assay involving the gal3 CRD domain. Furthermore, gal3 stimulated the TREM2-DAP12 signaling pathway. In conclusion, we provide evidence that gal3 is a central regulator of microglial immune response in AD. It drives proinflammatory activation and Aβ aggregation, as well as acting as an endogenous ligand to TREM2, a key receptor driving microglial response under disease conditions. Gal3 inhibition may, hence, be a potential pharmacological approach to counteract AD.
Biomass segregation between biofilm and flocs improves the control of nitrite-oxidizing bacteria in mainstream partial nitritation and anammox processes
Authors: Laureni Michele , Weissbrodt David G. , Villez Kris , Robin Orlane , de Jonge Nadieh , Rosenthal Alex , Wells George , Nielsen Jeppe Lund , Morgenroth Eberhard , Joss Adriano 
Published: December 2018

AbstractThe control of nitrite-oxidizing bacteria (NOB) challenges the implementation of partial nitritation and anammox (PN/A) processes under mainstream conditions. The aim of the present study was to understand how operating conditions impact microbial competition and the control of NOB in hybrid PN/A systems, where biofilm and flocs coexist. A hybrid PN/A moving-bed biofilm reactor (MBBR; also referred to as integrated fixed film activated sludge or IFAS) was operated at 15 °C on aerobically pre-treated municipal wastewater (23 mgNH4-N·L−1). Ammonium-oxidizing bacteria (AOB) and NOB were enriched primarily in the flocs, and anammox bacteria (AMX) in the biofilm. After decreasing the dissolved oxygen concentration (DO) from 1.2 to 0.17 mgO2·L−1 - with all other operating conditions unchanged - washout of NOB from the flocs was observed. The activity of the minor NOB fraction remaining in the biofilm was suppressed at low DO. As a result, low effluent NO3− concentrations (0.5 mgN·L−1) were consistently achieved at aerobic nitrogen removal rates (80 mgN·L−1·d−1) comparable to those of conventional treatment plants. A simple dynamic mathematical model, assuming perfect biomass segregation with AOB and NOB in the flocs and AMX in the biofilm, was able to qualitatively reproduce the selective washout of NOB from the flocs in response to the decrease in DO-setpoint. Similarly, numerical simulations indicated that flocs removal is an effective operational strategy to achieve the selective washout of NOB. The direct competition for NO2− between NOB and AMX - the latter retained in the biofilm and acting as a “NO2-sink” - was identified by the model as key mechanism leading to a difference in the actual growth rates of AOB and NOB (i.e., μNOB < μAOB in flocs) and allowing for the selective NOB washout. Experimental results and model predictions demonstrate the increased operational flexibility, in terms of variables that can be easily controlled by operators, offered by hybrid systems as compared to solely biofilm systems for the control of NOB in mainstream PN/A applications.HighlightsHybrid PN/A systems provide increased operational flexibility for NOB controlAOB and NOB enrich primarily in the flocs, and AMX in the biofilm (“NO2-sink”)AMX use NO2− allowing to differentiate AOB and NOB growth ratesA decrease in DO or an increase in floc removal leads to selective NOB washout from flocsThe activity of the minor NOB fraction in the biofilm is suppressed at limiting DO
Evolution of the HIV-1 RRE during natural infection reveals nucleotide changes that correlate with altered structure and increased activity over time
Authors: Sherpa Chringma , Jackson Patrick E. H. , Gray Laurie R. , Anastos Kathryn , Le Grice Stuart F. J. , Hammarskjold Marie-Louise , Rekosh David 
Published: December 2018

ABSTRACTAbstractThe HIV-1 Rev Response Element (RRE) is acis-acting RNA element characterized by multiple stem-loops. Binding and multimerization of the HIV Rev protein on the RRE promotes nucleocytoplasmic export of incompletely spliced mRNAs, an essential step in HIV replication. Most of our understanding of the Rev-RRE regulatory axis comes from studies of lab-adapted HIV clones. However, in human infection, HIV evolves rapidly and mechanistic studies of naturally occurring Rev and RRE sequences are essential to understanding this system. We previously described the functional activity of two RREs found in circulating viruses in a patient followed during the course of HIV infection. The “early” RRE was less functionally active than the “late” RRE despite differing in sequence by only four nucleotides. In this study, we describe the sequence, function, and structural evolution of circulating RREs in this patient using plasma samples collected over six years of untreated infection. RRE sequence diversity varied over the course of infection with evidence of selection pressure that led to sequence convergence as disease progressed. An increase in RRE functional activity was observed over time, and a key mutation was identified that correlates with a major conformational change in the RRE and increased functional activity. Additional mutations were found that may have contributed to increased activity as a result of greater Shannon entropy in RRE stem-loop II, which is key to primary Rev binding.ImportanceHIV-1 replication requires interaction of the viral Rev protein with acis-acting regulatory RNA, the Rev Response Element (RRE), whose sequence changes over time during infection within a single host. In this study, we show that the RRE is subject to selection pressure and that RREs from later time points in infection tend to have higher functional activity. Differences in RRE functional activity are attributable to specific changes in RNA structure. Our results suggest that RRE evolution during infection may be important for HIV pathogenesis and that efforts to develop therapies acting on this viral pathway should take this into account.
Dissociated cortical phase- and amplitude-coupling patterns in the human brain
Authors: Siems Marcus , Siegel Markus 
Published: December 2018

AbstractCoupling of neuronal oscillations may reflect and facilitate the communication between neuronal populations. Two primary neuronal coupling modes have been described: phase-coupling and amplitude-coupling. Theoretically, both coupling modes are independent, but so far, their neuronal relationship remains unclear. Here, we combined MEG, source-reconstruction and simulations to systematically compare cortical phase-coupling and amplitude-coupling patterns in the human brain. Importantly, we took into account a critical bias of amplitude-coupling measures due to phase-coupling. We found differences between both coupling modes across a broad frequency range and most of the cortex. Furthermore, by combining empirical measurements and simulations we ruled out that these results were caused by methodological biases, but instead reflected genuine neuronal amplitude coupling. Overall, our results suggest that cortical phase- and amplitude-coupling patterns are non-redundant, which may reflect at least partly distinct neuronal mechanisms. Furthermore, our findings highlight and clarify the compound nature of amplitude coupling measures.HighlightsSystematic comparison of cortical phase- and amplitude-coupling patternsDemonstration of genuine amplitude coupling independent of phase coupling biasAmplitude- and phase coupling patterns differ across many cortical regions and frequencies
Species-specific strategies increase unpredictability of escape flight in eared moths
Authors: Hügel Theresa , Goerlitz Holger R. 
Published: December 2018

ABSTRACTMany prey animals form mixed-species groups. Mixed-species groups provide various benefits ranging increased food intake to increased chance of predator detection. The escape-tactic diversity hypothesis predicts another benefit. It postulates that the overall unpredictability of evasive movement is increased if multiple species with different evasive tactics mix, resulting in enhanced predator protection for the whole group. Echolocating bats and eared moths are a textbook example of predator-prey interactions. Moths exhibit evasive flight with diverse tactics; however, the variability of their evasive flight within and between species has never been systematically quantified. We therefore recorded flight strength of eight moth species in response to the same level of simulated bat predation. We show species-specific and size-independent differences in both overall flight strength and in change of flight strength over time, confirming the escape-tactic diversity hypothesis for eared moths. Additionally, we show strong inter-individual differences in evasive flight within some species. This diversity in escape tactic between eared moths increases the overall unpredictability of their evasive flight in mixed-species groups, likely providing better protection against predatory bats for the single individual.
Structural venomics: evolution of a complex chemical arsenal by massive duplication and neofunctionalization of a single ancestral fold
Authors: Pineda Sandy S. , Chin Yanni K-Y. , Undheim Eivind A.B. , Senff Sebastian , Mobli Mehdi , Dauly Claire , Escoubas Pierre , Nicholson Graham M. , Kaas Quentin , Mattick John S. , King Glenn F. 
Published: December 2018

AbstractSpiders are the most successful venomous animals on the planet, with more than 47,000 extant species. Most spider venoms are dominated by disulfide-rich peptides (DRPs) with a diverse range of pharmacological activities. Although some venoms contain thousands of unique peptides, little is known about the mechanisms used to generate such complex chemical arsenals. We used a combined transcriptomic, proteomic and structural biology approach to demonstrate that the lethal Australian funnel-web spider produces 33 superfamilies of venom peptides and proteins, more than described for any other arachnid. We show that 15 of the 26 DRP superfamilies form an ultra-stable inhibitor cystine knot motif, and that these DRPs are the major contributor to the diversity of the venom peptidome. NMR data reveal that most of these DRPs are structurally related and range in complexity from simple to highly elaborated knottin domains that likely evolved from a single ancestral fold.