bluuebunny/crossref_metadata_2025_split huggingface dataset:
Yellow Perch Sperm Motility, Cryopreservation, and Viability of Resulting Larvae and Juveniles
Authors: Miller Mackenzie E , Kemski Megan , Grayson John D , Towne Kristen , Dabrowski Konrad 
Published: February 2018

Abstract
The sperm of Yellow Perch Perca flavescens of two different age-classes, age 0 and 3, were cryopreserved using two different cryoprotectants (dimethylsulfoxide [DMSO] and methanol [MetOH]) with two freezing methods (pellet and vial). The viability and quality of the progenies obtained from fertilization with cryopreserved sperm were then examined. The motility of Walleye Sander vitreus sperm was examined following cryopreservation and ultraviolet (UV) irradiation, with the aim of using heterologous cryopreserved sperm to inseminate Yellow Perch eggs to ensure gynogenesis. The first experiment compared the motility of fresh sperm and pellet method cryopreserved sperm devoid of salmon seminal plasma. Despite the high motility of fresh sperm—75% and 100%—of males age 0 and 3, respectively, the postthaw motility of seminal plasma devoid cryopreserved sperm was 0%. The second experiment addressed the efficiency of pellet and vial freezing methods with DMSO and MetOH supplemented with salmon seminal plasma. Cryopreserved sperm was thawed and motility measured. Sperm motility was not significantly different between pellet (13.3 ± 10.4%) and vial (10.3 ± 12.9%) methods in the absence of sperm extender; however, sperm motility of the pellet method was further improved (20 ± 8.7%) with the addition of sperm extender after thawing, while motility of the vial method sperm (10 ± 7.1%) was not. Cryopreserved sperm was further evaluated based on fertilization rate and ultimate survival and growth of larvae and juveniles through 14 d posthatch. Effects of UV exposure on fresh and pellet method cryopreserved sperm following UV irradiation were also examined. The motility of control sperm cryopreserved with DMSO and MetOH, and UV-exposed sperm cryopreserved with DMSO decreased from 100% motility before cryopreservation (fresh sperm) to 75% following cryopreservation, while UV-exposed sperm cryopreserved with MetOH decreased from 100% to 50%. This experiment provides significant new data to improve the effectiveness of straightforward cryopreservation techniques for Yellow Perch.
Earthy and Musty Off-Flavor Episodes in Catfish Split-Pond Aquaculture Systems
Authors: Schrader Kevin K , Tucker Craig S , Brown Travis W , Whitis Gregory N 
Published: February 2018

Abstract
The interest and use of variations of partitioned aquaculture systems (PAS) by the catfish farming industry in the southeastern USA continues to grow. Split-pond systems, one type of PAS, are designed to improve the management of dissolved oxygen levels and fish waste products (e.g., ammonia) compared with conventional earthen ponds that have been used for many decades. While recent studies of split-pond systems have focused on design, water flow rates, and management practices, there has not been any assessment of the occurrences and intensities of common “off-flavor” episodes in these split-ponds. For the current study, pond water samples were collected from split-ponds at a commercial fish farm in western Alabama and at a research facility in western Mississippi approximately every 3–4 weeks during the fish grow-out period (May to November) during 2014 and 2015. In addition, fillet samples from hybrid catfish (Channel Catfish Ictalurus punctatus × Blue Catfish Ictalurus furcatus) were obtained in late summer and autumn from these same split-ponds. Concentrations of the “earthy” and “musty” off-flavor compounds geosmin and 2-methylisoborneol (MIB), respectively, in water and fillet samples were determined. Sensory analysis was also performed on catfish fillets collected during 2014. The highest geosmin and MIB concentrations detected in split-pond water during the study were 898 and 22,651 ng/L, respectively. Geosmin and MIB concentrations in split-pond water were often higher in summer and autumn, and concentrations were similar to those reported previously for conventional ponds. The highest geosmin and MIB concentrations detected in fillets during the study were 1,712 and 29,851 ng/kg, respectively. Concentrations of these compounds in fillets and the description and intensities determined via sensory analysis were similar to those reported previously for off-flavor catfish from conventional ponds. Accordingly, catfish farmers using split-ponds can use similar management approaches as those used to mitigate earthy and musty off-flavor problems in catfish raised in conventional ponds (e.g., applications of algicides).
Evaluation of Poultry By-Product Meal as an Alternative to Fish Meal in the Diet of Juvenile Black Sea Bass Reared in a Recirculating Aquaculture System
Authors: Dawson Matthew R , Alam Md Shah , Watanabe Wade O , Carroll Patrick M , Seaton Pamela J 
Published: February 2018

Abstract
A feeding trial was conducted to determine the maximum substitution limits of poultry by-product meal (PBM; 66% crude protein) protein for fish meal (FM; 59% crude protein) protein in the diet of juvenile Black Sea Bass Centropristis striata (family Serranidae). Eight isonitrogenous (44% crude protein) and isolipidic (13% crude lipid) diets were formulated to replace FM protein with PBM protein at 0 (control), 40%, 50%, 60%, 70%, 80%, 90%, and 100% in Black Sea Bass diets. Diets were fed twice daily to triplicate groups of juveniles (initial mean weight = 1.2 g) to apparent satiation for 8 weeks in a recirculating aquaculture system. Final survival was excellent (95–100%) in all diet treatments, with no significant differences. No significant differences in body weight gain (BWG) were observed in fish fed the 40–90% PBM protein diets (1,136–1,357%) compared with the control diet (1,307%). However, BWG of fish fed the 100% PBM protein diet (1,045%) was significantly lower than in the control group. Regression analysis with BWG indicated that PBM protein can replace FM protein in Black Sea Bass diets at levels as high as 81.8%, with no reduction in fish growth performance. For fish fed diets with up to 90% PBM protein, feed conversion (1.08–1.17) and protein efficiency ratios (2.01–2.14) were not significantly different from fish fed a control 100% FM-protein-based diet (0.99 and 2.29, respectively). Apparent digestibility coefficients of dietary protein remained high (81.6–87.0%) under all levels of FM replacement with PBM protein. After the feeding trial, whole body and muscle protein content and the concentrations of whole body n-3 polyunsaturated fatty acids showed no significant differences among the treatments at FM protein replacement levels up to 90%. Poultry by-product meal is a promising alternative protein source for sustainable diet development in Black Sea Bass.
Environmental Tax, Polluting Plants’ Strategies and Effectiveness: Evidence from China
Authors: He Pan , Zhang Bing 
Published: February 2018

AbstractAlthough environmental taxes have become a popular policy tool, their effectiveness for pollution control and impact on the compliance strategies of agents remains questionable. This research uses a quasi‐experimental design to examine the effectiveness of the Pay for Permit policy, an environmental tax that has been imposed on water pollution emissions in Lake Tai Basin, Jiangsu, China, since 2009. A plant‐level panel dataset from 2007 to 2010 is used for both difference‐in‐differences and difference‐in‐difference‐in‐differences analyses to compare the pollution discharge, pollution abatement, and pollution generation of policy participants and control groups. The results indicate that treated plants reduce their emissions by about 40 percent after two years of the policy implementation. Thus, the policy generated approximately a 7 percent decrease in the industrial chemical oxygen demand emission in the entire Lake Tai Basin based on the emission level of 2007. Pollution is primarily reduced via end‐of‐pipe abatement instead of cleaner production. Our results show the effectiveness of environmental taxes in controlling industrial pollution, and indicate that the tax may not motivate the adoption of innovative techniques in the short term.
The molecular pathology of cancer: from pan‐genomics to post‐genomics
Authors: Huntsman David G , Ladanyi Marc 
Published: February 2018

AbstractAs the cancer genomics of most major cancer types have been comprehensively catalogued over the past decade through a variety of national and international efforts, the delineation of cancer subtypes has been refined, and our understanding of critical cancer drivers and of the potentially targetable vulnerabilities that they create has grown tremendously. The 2018 Annual Review Issue of the Journal of Pathology provides in‐depth assessments of how these pan‐genomic approaches have enabled advances in cancer classification, targeted therapy selection, and assessment of cancer progression, all of which are now genomically informed, using several cancer types as examples. Beyond these areas of by now conventional pan‐genomic tumour analysis, there are also reviews of diverse ‘post‐genomic’ areas, such as the analysis of circulating free tumour DNA in plasma, concurrent germline cancer predisposition profiling in the setting of apparently sporadic cancer, genetic alterations in epigenetic control and DNA repair, proteomics of tumour heterogeneity, computational pathology, and the roles of the cellular stress response and the microbiome in human cancers. As we are able to derive more and more biologically useful information from diverse human biospecimens, these many advances are informing and transforming the practice of cancer pathology. Copyright © 2018 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Early hemodynamic changes after fetal aortic stenosis valvuloplasty predict biventricular circulation at birth
Authors: Prosnitz Aaron R. , Drogosz Monika , Marshall Audrey C. , Wilkins‐Haug Louise E. , Benson Carol B. , Sleeper Lynn A. , Tworetzky Wayne , Friedman Kevin G. 
Published: February 2018

AbstractObjectiveTo describe the early hemodynamic changes after fetal aortic valvuloplasty (FAV) for evolving hypoplastic left heart syndrome due to mid‐gestational aortic stenosis and to assess whether these early changes predict biventricular (BiV) circulation at neonatal discharge.MethodWe retrospectively reviewed all technically successful FAV cases resulting in live birth between 2000 and 2015 (n = 93, 45% BiV circulation at neonatal discharge). Paired testing methods were used to compare pre‐intervention and post‐intervention measures of left ventricular hemodynamics. Logistic regression was used to determine whether these changes were predictive of post‐natal outcome.ResultsMeasures of left heart physiology were markedly abnormal pre‐FAV and improved significantly post‐FAV. No subjects had systolic antegrade transverse aortic arch flow pre‐FAV and 65% of subjects had antegrade flow post‐FAV. The number of subjects with abnormal left‐to‐right patent foramen ovale flow decreased, and the number with biphasic mitral valve inflow increased. The median left ventricular ejection fraction improved after intervention. Amongst the pre‐post changes, gaining partially or exclusively antegrade systolic arch flow was the most significant independent predictor of BiV circulation (OR 9.80 and 19.83, respectively, both P < 0.001).ConclusionTechnically successful FAV is associated with immediate improvements in left heart physiology that are predictive of BiV circulation at neonatal discharge.
Membranotropic peptides mediating viral entry
Authors: Falanga Annarita , Galdiero Massimiliano , Morelli Giancarlo , Galdiero Stefania 
Published: February 2018

AbstractThe means used by enveloped viruses to bypass cellular membranes are well characterized; however, the mechanisms used by non‐enveloped viruses to deliver their genome inside the cell remain unresolved and poorly defined. The discovery of short, membrane interacting, amphipathic or hydrophobic sequences (known as membranotropic peptides) in both enveloped and non‐enveloped viruses suggests that these small peptides are strongly involved in breaching the host membrane and in the delivery of the viral genome into the host cell. Thus, in spite of noticeable differences in entry, this short stretches of membranotropic peptides are probably associated with similar entry‐related events. This review will uncover the intrinsic features of viral membranotropic peptides involved in viral entry of both naked viruses and the ones encircled with a biological membrane with the objective to better elucidate their different functional properties and possible applications in the biomedical field.
Development of a solid‐phase traceless‐Ugi multicomponent reaction for backbone anchoring and cyclic peptide synthesis
Authors: Jobin Steve , Beaumont Catherine , Biron Eric 
Published: February 2018

AbstractA new one‐pot methodology to anchor peptides by their backbone to a solid support using an isocyanide‐based multicomponent reaction is described. The approach uses a microwave‐assisted Ugi four‐component reaction to simultaneously condense and bind an N‐protected amino acid and an amino ester to a supported aldehyde. Afterward, the generated backbone anchored dipeptide can be used in solid‐phase peptide synthesis to prepare head‐to‐tail cyclic peptides. Moreover, we also show that peptide fragment ligation can be performed with the described one‐pot anchoring. The backbone anchored peptides can be efficiently released from the resin by microwave‐assisted acidolysis with trifluoroacetic acid. This straightforward one‐pot anchoring approach was also applied to condense fragments and prepare a variety of linear and macrocyclic peptides.
Any Thing for Anyone? A New Digital Divide in Internet‐of‐Things Skills
Authors: van Deursen Alexander J. A. M. , Mossberger Karen 
Published: February 2018

AbstractThe “Internet‐of‐Things” (IoT) promises social benefits across a range of policy areas, such as energy, health, transportation, public safety, and environmental policy, but attention to the skills needed by individuals who use it will be an important issue for public policy, in order to ensure full exploitation of these technologies and to avoid unintended consequences. We argue that comparative advantages of the IoT to people will vary based on differentiated skills and resources, enabling smaller groups of people to benefit, and disadvantaging others in new ways. This need for renewed attention to digital skills and knowledge might at first seem paradoxical, given that many of these technologies operate autonomously and behind the scenes. We discuss evolving digital technologies and related skills, and explain from a systems perspective how the IoT differs from prior technologies, with a premium placed on user knowledge and strategic skills. Finally, we bring together the issues of the digital divide and IoT skills, and set an agenda for future IoT public policy and research.
Health‐related quality of life and care satisfaction outcomes: Informing psychosocial oncology care among Latina and African‐American young breast cancer survivors
Authors: Ashing Kimlin Tam , George Marshalee , Jones Veronica 
Published: February 2018

AbstractObjectiveWhen breast cancer occurs in young women, the medical, physical, psychosocial, and overall impacts can be more severe warranting targeted medical and psychosocial oncology care. Yet, despite their risk for poorer survival and survivorship outcomes, little research has focused on this group with critical gaps concerning ethnic minorities who are particularly medically vulnerable. Therefore, this preliminary study examined demographic characteristics and patient centered outcomes, ie, health‐related quality of life (HRQOL), quality care satisfaction to inform targeted psychosocial oncology care among African‐American and Latinas young breast cancer survivors (YBCS).MethodsA total of 116 African‐American and Latina YBCS aged ≥21 to 50 years were recruited from cancer registries and community agencies. Based on prior research and the literature, Latinas were categorized into English language proficient (ELP) and Spanish language proficient (SLP) based on their choice of language to conduct the study including completion of the measures.ResultsSLP Latinas reported lower educational attainment and income (P < 0.001) and were more likely to report having a mastectomy (P < 0.01) but less likely to report breast reconstruction (P < 0.05). Satisfaction with care was correlated with patient‐provider communication and overall HRQOL (P < 0.01) and physical, social/family, emotional and functional wellbeing (P < 0.01). SLP Latinas had lower emotional wellbeing than African‐American YBCS (P < 0.01) and lower functional wellbeing than ELP Latina YBCS (P < 0.05). SLP Latina YBCS were less satisfied with their care compared with African‐American and ELP Latina YBCS (P < 0.01). Financial toxicity seems to directly influence both access to care and quality care and survivorship outcomes.ConclusionsInvestigating demographic characteristics and medical outcomes including HRQOL outcomes and satisfaction with care among ethnic minority YBCS is needed to advance the science as well as assist health professionals with precision care delivery. Greater translational and patient‐centered research must focus on at‐risk population such as YBCS to inform precision psychosocial oncology care and reduce health disparities.
Training using a new multidirectional reach tool improves balance in individuals with stroke
Authors: Khumsapsiri Numpung , Siriphorn Akkradate , Pooranawatthanakul Kanokporn , Oungphalachai Tanyarut 
Published: February 2018

AbstractBackground and purposePrevious studies suggested that limits of stability (LOS) training with visual feedback using commercial equipment could be used to improve balance ability in individuals with stroke. However, this system is expensive. In this study, we created a new tool from inexpensive elements based on LOS training using visual feedback. The aim of this study was to investigate the effect of training using a new multidirectional reach tool on balance in individuals with stroke.MethodsA single‐blind randomized control trial was conducted. Individuals with stroke (n = 16; age range 38–72 years) were recruited. Participants in the experimental group were trained with the multidirectional reach training for 30 min and conventional physical therapy for 30 min per day, 3 days a week for 4 weeks. Participants in the control group received conventional physical therapy for 30 min per day, 3 days a week for 4 weeks. The outcomes were LOS, weight‐bearing squat, and Fullerton Advanced Balance scale. All of the outcome measures were measured at pretraining, post‐training, and 1 month follow‐up.ResultsAt post‐training and 1‐month follow‐up, the participants in the experimental group had an improvement of dynamic balance than the control group. Furthermore, the activity assessment by Fullerton Advanced Balance scale was more improved at 1 month follow‐up in the experimental group than control group.ConclusionThe results of this study provide evidence that training using a new multidirectional reach tool is effective for improving balance in individuals with stroke.
Multisensory exercise programme improves cognition and functionality in institutionalized older adults: A randomized control trial
Authors: Moreira Natália Boneti , Gonçalves Gabriele , da Silva Talihina , Zanardini Francisco Ernesto Halila , Bento Paulo Cesar Barauce 
Published: February 2018

AbstractAimThe aim of this study was to verify the effects of a multisensory exercise programme on the cognition and functionality of institutionalized older adults.MethodsForty‐five volunteers were randomly allocated to 2 groups, the multisensory exercise programme (n = 24) and the control group that received no treatment (n = 21). The programme consisted of 3 50‐min sessions of progressive exercises per week for 16 weeks that challenged their strength, balance, coordination, multisensory stimulation, and flexibility in different tasks. Cognition (Montreal Cognitive Assessment), balance (Berg Scale), mobility (Timed Up and Go), and functional performance (Physical Performance Test) were measured preintervention and postintervention. Statistical analyses were performed using Student's t test and 2‐way ANOVA.ResultsThe multisensory exercise programme showed statistically significant improvements (p < .05) on cognition (effect size [ES]: 0.92), balance (ES: 0.77), mobility (ES: 0.51), and functional performance (ES: 0.86) as compared with the control group, which showed no statistical significant differences at the postintervention time point.ConclusionsThe multisensory exercise programme improved the cognition and functionality of institutionalized older adults. The introduction of a motor and multisensory‐based approach in care routines may improve residents' health and engagement to the environment.
The prognosis of different distant metastases pattern in prostate cancer: A population based retrospective study
Authors: Shou Jiafeng , Zhang Qi , Wang Shuai , Zhang Dahong 
Published: February 2018

BackgroundThe present of metastases is a poor prognostic factor in prostate cancer, but the prognostic impact of different distant metastases pattern is unclear. The aim of this study is to investigate the impact of different distant metastases pattern on the survival of patients with stage IV prostate cancer.MethodsData queried for this study include prostate cancer (2010‐2014) from the Surveillance, Epidemiology, and End Results (SEER) program. Metastatic distribution information was provided for bone, brain, liver and lung. The overall survival was calculated by the Kaplan‐Meier method. Multivariable Cox regression models were used to analyze survival outcome and risk factors.ResultsA total of 265 900 eligible patients were identified from SEER database. Among these patients, stage of IV prostate cancer accounted for 7.53% (20 034/265 900) at diagnosis. Patients who suffered metastasis to either one of the four organs occupied 61.24% (12 268/20 034) in stage of IV patients. Comparing with other three single metastases, the patients with liver metastasis exhibited worst OS whose mean survival was 17.529 months (P < 0.001). The mean survival of metastases with bong and lung was 25.238 months, which was the best survival of the six forms with two metastatic sites (P < 0.001). The results of univariate survival analysis showed that metastatic forms, race, N‐classification and differentiated grade did not have impact on the overall survival of patients with three metastatic sites (all, P > 0.05).ConclusionsIn analysis of both one and two metastatic sites, patients with liver metastasis seemed to have worse survival outcome. On the other hand, bone metastasis had better outcome than other three visceral metastases. Knowledge of these differences in metastatic patterns may help to better guide pre‐treatment evaluation of prostate cancer and make determination regarding curative‐intent interventions.