bluuebunny/crossref_metadata_2025_split huggingface dataset:
A new microdispersed albumin derivative potentially useful for radio-guided surgery of occult breast cancer lesions
Authors: Caviglioli Gabriele , Chinol Marco , Baldassari Sara , Garaboldi Lucia , Zuccari Guendalina , Petretto Andrea , Drava Giuliana , Sinico Chiara , Paganelli Giovanni 
Published: April 2019

AbstractThis paper describes a new nuclear imaging agent, 2-(4-isothiocyanatobenzyl)−1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid of human albumin (HAC), potentially suitable for application in the Radio-guided Occult Lesion Localization (ROLL) of non-palpable mammalian cancerous lesions, as a tool to overtake the short radio-signal half-life of the technetium-99m based radiopharmaceutical currently used. This conjugate is a microsized powder aggregate, water-insoluble between pH 3 and 8.5, obtained by conjugating the protein with the macrocyclic chelating agent DOTA through a one-pot reaction in aqueous medium. The product has been fully characterized and is stable to the thermal conditions adopted for labeling; after radiolabeling with longer half-life radionuclides such as 177Lu or 111In, it has shown radiochemical purity (RCP) >90% and resulted stable when stored in saline or plasma for 6 days at 37 °C. A μPET/CT study, performed in vivo on adult female rats, showed that the radioactivity of HAC labeled with 64Cu remained located in the mammary glands for at least 40 h, without diffusion or drainage in healthy tissues or in the lymphatic circulation. This new imaging agent might make the ROLL procedure more accessible, safe and flexible, promoting a significant time and cost reduction of this intervention. Moreover, HAC might also be used in other radio-guided surgical procedures in oncology.
East China Sea increasingly gains limiting nutrient P from South China Sea
Authors: Huang Ting-Hsuan , Chen Chen-Tung Arthur , Lee Jay , Wu Chau-Ron , Wang You-Lin , Bai Yan , He Xianqiang , Wang Shu-Lun , Kandasamy Selvaraj , Lou Jiann-Yuh , Tsuang Ben-Jei , Chen Hsien-Wen , Tseng Ruo-Shan , Yang Yiing Jang 
Published: April 2019

AbstractThe Taiwan Strait (TS) directly connects two of the richest fishing grounds in the world - the East China Sea (ECS) and the South China Sea (SCS). Carbon and nutrient supplies are essential for primary production and the Yangtze River is an important source for the ECS. However the ECS is severely P-limited. The TS transports an order of magnitude more carbon and a factor of two more phosphate (P) to the ECS than the Yangtze River does. To evaluate the temporal variability of these supplies, the total alkalinity (TA), dissolved inorganic carbon (DIC), nitrate plus nitrite (N), P, and silicate (Si) fluxes through the TS were estimated using empirical equations for these parameters and the current velocity, which was estimated using the Hybrid Coordinate Ocean Model (HYCOM). These empirical equations were derived from in situ salinity and temperature and measured chemical concentrations that were collected during 57 cruises (1995–2014) with a total of 2096 bottle samples. The 24-month moving averages of water, carbon, and nutrient fluxes significantly increase with time, so does the satellite chlorophyll a concentration. More importantly, the increased supply of the badly needed P from the TS is more than that from the Yangtze River.
Mechanized transplanting with side deep fertilization increases yield and nitrogen use efficiency of rice in Eastern China
Authors: Zhu Conghua , Xiang Jing , Zhang Yuping , Zhang Yikai , Zhu Defeng , Chen Huizhe 
Published: April 2019

AbstractThe application of nitrogen (N) fertilizer deep in soil at the same time as mechanical transplanting of rice seedlings is an effective alternative to conventional broadcasting of fertilizer, but its effects on yields and profitability have not been analysed in detail. Here, we analysed the effects of a side deep application of N fertilizer at transplanting on the N uptake, N use efficiency (NUE), grain yield, and economic profitability of two rice (Oryza sativa L.) cultivars; Yongxian15 (early season) and Yongyou1540 (middle/late season). In the field experiments, two types of N fertilizer (urea (U) and controlled-release urea (CRU)) were surface broadcasted manually (B) or mechanically fertilized at 5.5 ± 0.5 cm soil depth (M) (UB, UM, and CRUM treatments, respectively). The blank control had no N fertilizer (N0). Each N-fertilizer treatment had similar effects on N uptake, grain yield, NUE, and economic profitability in the early, middle, and late seasons. Compared with manually applied fertilizer, mechanically applied fertilizer increased grain yield and NUE in both cultivars. In Yongxian15 and Yongyou1540, the mechanical side deep application of N-fertilizer increased the N recovery efficiency by 62.50–91.57% and 24.38–64.24%, respectively, the N agronomy efficiency by 33.65–63.14% and 22.64–44.70%, respectively; and the grain yield by 6.30–11.64% and 6.23–13.11%, respectively. The CRUM treatments had the highest benefit–cost ratio because of high gross returns and low fertilization costs. The mechanized side deep application of N fertilizer can increase the efficiency and profitability of rice production.
Colorimetric Gas Sensing Washable Threads for Smart Textiles
Authors: Owyeung Rachel E. , Panzer Matthew J. , Sonkusale Sameer R. 
Published: April 2019

AbstractA fabrication method for a stable entrapment of optically responsive dyes on a thread substrate is proposed to move towards a detection system that can be integrated into clothing. We use the dyes 5,10,15,20-Tetraphenyl-21H,23H-porphine manganese(III) chloride (MnTPP), methyl red (MR), and bromothymol blue (BTB), for a proof-of-concept. Our optical approach utilizes a smartphone to extract and track changes in the red (R), green (G) and blue (B) channel of the acquired images of the thread to detect the presence of an analyte. We demonstrate sensing of 50–1000 ppm of vapors of ammonia and hydrogen chloride, components commonly found in cleaning supplies, fertilizer, and the production of materials, as well as dissolved gas sensing of ammonia. The devices are shown to be stable over time and with agitation in a centrifuge. This is attributed to the unique dual step fabrication process that entraps the dye in a stable manner. The facile fabrication of colorimetric gas sensing washable threads is ideal for the next generation of smart textile and intelligent clothing.
Personalized repetitive transcranial magnetic stimulation temporarily alters default mode network in healthy subjects
Authors: Singh Aditya , Erwin-Grabner Tracy , Sutcliffe Grant , Antal Andrea , Paulus Walter , Goya-Maldonado Roberto 
Published: April 2019

AbstractHigh frequency repetitive transcranial magnetic stimulation (HF-rTMS) delivered to the left dorsolateral prefrontal cortex (DLPFC) is an effective treatment option for treatment resistant depression. However, the underlying mechanisms of a full session of HF-rTMS in healthy volunteers have not yet been described. Here we investigated, with a personalized selection of DLPFC stimulation sites, the effects driven by HF-rTMS in healthy volunteers (n = 23) over the default mode network (DMN) in multiple time windows. After a complete 10 Hz rTMS (3000 pulses) session, we observe a decrease of functional connectivity between the DMN and the subgenual Anterior Cingulate Cortex (sgACC), as well as the ventral striatum (vStr). A negative correlation between the magnitude of this decrease in the right sgACC and the harm avoidance domain measure from the Temperament and Character Inventory was observed. Moreover, we identify that coupling strength of right vStr with the DMN post-stimulation was proportional to a decrease in self-reports of negative mood from the Positive and Negative Affect Schedule. This shows HF-rTMS attenuates perception of negative mood in healthy recipients in agreement with the expected effects in patients. Our study, by using a personalized selection of DLPFC stimulation sites, contributes understanding the effects of a full session of rTMS approved for clinical use in depression over related brain regions in healthy volunteers.
RNA interference and validation of reference genes for gene expression analyses using qPCR in southern pine beetle, Dendroctonus frontalis
Authors: Kyre Bethany R. , Rodrigues Thais B. , Rieske Lynne K. 
Published: April 2019

AbstractRNA interference (RNAi) is a highly specific gene-silencing mechanism that can cause rapid insect mortality when essential genes are targeted. RNAi is being developed as a tool for integrated pest management of some crop pests. Here we focus on an aggressive forest pest that kills extensive tracts of pine forests, the southern pine beetle (SPB),Dendroctonus frontalis. We sought to identify reference genes for quantitative real-time PCR (qPCR) and validate RNAi responses in SPB by mortality and gene silencing analysis. Using an adult beetle feeding bioassay for oral ingestion of dsRNA, we measured the expression and demonstrated knockdown of target genes as well as insect mortality after ingestion of target genes. Our study validates reference genes for expression analyses and demonstrates highly effective RNAi responses in SPB, with RNAi response to some target dsRNAs causing 100% beetle mortality after ingestion.
Whole exome sequencing identifies novel USH2A mutations and confirms Usher syndrome 2 diagnosis in Chinese retinitis pigmentosa patients
Authors: Ng Tsz Kin , Tang Wenyu , Cao Yingjie , Chen Shaowan , Zheng Yuqian , Xiao Xiaoqiang , Chen Haoyu 
Published: April 2019

AbstractRetinitis pigmentosa (RP) is a common phenotype in multiple inherited retinal dystrophies (IRD). Disease gene identification can assist the clinical diagnosis of IRD patients for better clinical management, treatment and counseling. In this study, we aimed to delineate and characterize the disease-causing mutations in Chinese familial and sporadic patients with initial diagnosis of RP. Four unrelated Chinese families and 118 sporadic RP patients were recruited for whole exome sequencing analysis. A total of 5 reported and 3 novel USH2A mutations were identified in four Chinese probands. The probands and their family members showed typical RP features and mild to severe hearing impairment, confirming the diagnosis of Usher syndrome 2 (USH). Moreover, 11 sporadic RP patients were identified to carry the compound heterozygous mutations in the USH2A gene, confirming the diagnosis of USH2. The patients carried the truncating mutations had a younger age of first visit than the patients carried only the missense mutations (p = 0.017). In summary, this study revealed 8 novel USH2A variants in Chinese familial and sporadic RP patients, assuring that whole exome sequencing analysis is an adequate strategy to facilitate the clinical diagnosis of USH from the sporadic RP patients.
Baseline impulsivity may moderate L-DOPA effects on value-based decision-making
Authors: Petzold Johannes , Kienast Annika , Lee Ying , Pooseh Shakoor , London Edythe D. , Goschke Thomas , Smolka Michael N. 
Published: April 2019

AbstractResearch has indicated a major role of dopamine in decision-making processes, but the underlying mechanisms remain largely unknown due to inconsistency in effects of dopaminergic drugs. To clarify the impact of dopamine on impulsive choice, we administered 150 mg L-DOPA to 87 healthy adults in a randomized, placebo-controlled, double-blind, crossover study, evaluating performance in four value-based decision-making tasks. We predicted that baseline impulsivity would moderate L-DOPA effects. In support of our hypothesis, L-DOPA had no main effect on impulsive choice, but reduced risk-seeking for gains in more-impulsive subjects. Because L-DOPA effects may be influenced by body weight, we repeated our analyses on data from half of the sample (n = 44) with lower weight, anticipating a stronger effect. In addition to the effect on risk-seeking for gains, low-weight participants also exhibited baseline-dependent effects of L-DOPA on loss aversion and delay discounting. Our results are consistent with the hypothesis of an inverted U-shaped dopamine function in which both low and high extremes of dopamine signaling are associated with high-impulsive choice. Consideration of differential baseline impulsivity and body weight may resolve previous seemingly paradoxical pharmacological results and might deepen our understanding of dopaminergic mechanisms underlying impulsivity.
Transcriptional profiling of Auricularia cornea in selenium accumulation
Authors: Li Xiaolin , Yan Lijuan , Li Qiang , Tan Hao , Zhou Jie , Miao Renyun , Ye Lei , Peng Weihong , Zhang Xiaoping , Tan Wei , Zhang Bo 
Published: April 2019

AbstractAuricularia corneais a widely cultivated edible fungus with substantial nutritive value. This study aimed to enrich the multifunctional bionutrient element selenium inA. corneato improve its quality and explore the accumulation of selenium in the fungus using high-throughput RNA-Seq technology. In general, the treatment group with a 100 µg/g supply of selenium outperformed the other treatment groups in terms of high yield, rich crude polysaccharides and a high total selenium concentration. Additional evidences demonstrated the budding and mature phases were two typical growth stages ofA. corneaand were important for the accumulation of selenium. Therefore, the budding and mature phase tissues ofA. corneain the treatment group with a 100 µg/g supply of selenium were used for transcriptome analysis and compared to those of a control group that lacked additional selenium. A total of 2.56 × 105 unigenes fromA. corneatranscriptome were assembled and annotated to five frequently used databases including NR, GO, KEGG, eggNOG and SwissProt. GO and KEGG pathway analysis revealed that genes involved in metabolic process and translation were up-expressed at the budding stage in response to selenium supplementation, including amino acid metabolism, lipid metabolism, ribosome. In addition, the differential gene expression patterns ofA. corneasuggested that the up-expressed genes were more likely to be detected at the budding stage than at the mature stage. These results provide insights into the transcriptional response ofA. corneato selenium accumulation.
Investigation of the adsorption capacity of the enterosorbent Enterosgel for a range of bacterial toxins, bile acids and pharmaceutical drugs
Authors: Howell Carol A. , Mikhalovsky Sergey V. , Markaryan Elena N. , Khovanov Alexander V. 
Published: April 2019

AbstractOral intestinal adsorbents (enterosorbents) are orally administered materials which pass through the gut where they bind (adsorb) various substances. The enterosorbent Enterosgel (Polymethylsiloxane polyhdrate) is recommended as a symptomatic treatment for acute diarrhoea and chronic diarrhoea associated with irritable bowel syndrome (IBS). Since 1980’s there have been many Enterosgel clinical trials, however, the detailed mechanism of Enterosgel action towards specific toxins and interaction with concomitantly administered medications has not been fully investigated. Ourin vitrostudy assessed the adsorption capacity of Enterosgel for bacterial enterotoxins and endotoxin, bile acids and interaction with the pharmaceutical drugs; Cetirizine and Amitriptyline hydrochloride. Our data demonstrate the good adsorption capacity of Enterosgel for bacterial toxins associated with gastrointestinal infection, with a lower than the comparator charcoal Charcodote capacity for bile acids whose levels can be raised in IBS patients. Adsorption capacity for the two drugs varied but was significantly lower than Charcodote. These findings suggest that the mechanism of Enterosgel action in the treatment of gastrointestinal infection or IBS is adsorption of target molecules followed by removal from the body. This therapy offers a drug free approach to prevention and treatment of infectious and chronic non-infectious diseases, where intestinal flora and endotoxemia play a role.
Metabolic stress controls mutant p53 R248Q stability in acute myeloid leukemia cells
Authors: Allende-Vega Nerea , Villalba Martin 
Published: April 2019

AbstractEliminating mutant p53 (mt p53) protein could be a useful strategy to treat mt p53 tumors and potentially improve the prognosis of cancer patients. In this study, we unveil different mechanisms that eliminate p53-R248Q, one of the most frequent mutants found in human cancers. We show that the Hsp90 inhibitor 17-AAG eliminates R248Q by stimulating macroautophagy under normal growth conditions. Metabolic stress induced by the pyruvate dehydrogenase kinase-1 (PDK1) inhibitor dichloroacetate (DCA) inhibits the macroautophagy pathway. This induces the accumulation of R248Q, which in addition further inhibits macroautophagy. Combination of DCA and 17-AAG further decreases the autophagy flux compared to DCA alone. Despite this, this co-treatment strongly decreases R248Q levels. In this situation of metabolic stress, 17-AAG induces the binding of p53-R248Q to Hsc70 and the activation of Chaperone-Mediated Autophagy (CMA), leading to higher R248Q degradation than in non-stress conditions. Thus, different metabolic contexts induce diverse autophagy mechanisms that degrade p53-R248Q, and under metabolic stress, its degradation is CMA-mediated. Hence, we present different strategies to eliminate this mutant and provide new evidence of the crosstalk between macroautophagy and CMA and their potential use to target mutant p53.
Reducing Mcl-1 gene dosage induces dopaminergic neuronal loss and motor impairments in Park2 knockout mice
Authors: Ekholm-Reed Susanna , Baker Robert , Campos Alexandre R. , Stouffer David , Henze Martha , Wolf Dieter A. , Loring Jeanne F. , Thomas Elizabeth A. , Reed Steven I. 
Published: April 2019

AbstractMutations in the PARK2 gene are associated with early onset Parkinsonism. The Park2−/− mouse, however, does not exhibit neurodegeneration or other Parkinson’s disease (PD) phenotypes. Previously, we discovered that translation of Mcl-1, a pro-survival factor, is upregulated in the Park2−/− mouse, suggesting a compensatory mechanism during development. Here we generated the Park2−/−Mcl-1+/− mouse and show that by reducing Mcl-1 gene dosage by 50%, the Park2−/− genotype is sensitized, conferring both dopaminergic neuron loss and motor impairments. We propose that this murine model could be a useful tool for dissecting PD etiology and developing treatment strategies against this neurodegenerative disease.
Role of Orexin-B/Orexin 2 receptor in myocardial protection
Authors: Imperatore Roberta , Cristino Luigia 
Published: April 2019

Abstract
Emerging evidence attributes to orexins/hypocretins (ORs) a protective function in the regulation of cardiovascular responses, heart rate, and hypertension. However, little is known about any direct effect of orexins in the heart function. This is of special relevance considering that cardiovascular diseases, including myocardial infarction and heart failure, are one of the major causes of mortality in the world. In the article published in Clinical Science (2018) (vol. 132, 2547–2564), Patel and colleagues investigated the role of orexins in myocardial protection. Intriguingly, they revealed a source of orexin-A (OR-A) and orexin-B (OR-B) in the heart and cardiomyocytes of the rat. More interestingly, these peptides exert a direct effect on the heart rate by acting in an autocrine/paracrine manner on their respective receptors (OXRs). Indeed, OR-B, but not OR-A, by acting through orexin receptor-2 (OX2R), exerts direct cardioprotective effects in heart failure models. OR-B/OX2R signalling enhances myosin light chain (MLC) and troponin-I (TnI) phosphorylation in a dose-dependent manner, leading to an increase in the strength of their twitch contraction. This effect is mediated by extracellular signal-regulated kinase 1/2 (ERK1/2) and Akt phosphorylation, both in the rat myocardial tissue and human heart samples. A negative correlation between OX2R expression and clinical severity of symptoms has been found in patients with heart failure. Thus, in addition to the known central effects of orexins/OX2R, the work of Patel and colleagues (Clinical Science (2018) 132, 2547–2564) reports a direct action of OR-B on the heart rate pinpointing to OX2R as a potential therapeutic target for prevention and treatment of cardiovascular disease.
Die klinische Anwendung von Milnacipran in Österreich und Deutschland
Authors: Hienert Marius , Ritter Philipp , Bauer Michael , Lanzenberger Rupert , Kasper Siegfried 
Published: April 2019

ZusammenfassungMilnacipran ist ein Antidepressivum aus der Gruppe der Serotonin-Noradrenalin-Wiederaufnahmehemmer, zu welcher auch Venlafaxin und Duloxetin gehören. Im Gegensatz zu den anderen beiden Vertretern dieser Gruppe bindet dieses Psychopharmakon in etwa gleich stark an den Serotonin- sowie Noradrenalintransporter und führt zu einer Erhöhung des jeweiligen Neurotransmitters außerhalb der Nervenzelle. In den USA wird Milnacipran zur Therapie des Fibromyalgie-Syndroms eingesetzt, während es in Europa zur Behandlung der Depression zugelassen ist. Milnacipran hat aufgrund seiner Pharmakokinetik und Pharmakodynamik besondere Eigenschaften, wobei die antriebssteigernde Wirkung, Verbesserung der sozialen und sexuellen Funktion, ein gutes Nebenwirkungsprofil sowie aufgrund von fehlender CYP450-Interaktion ein geringes Wechselwirkungspotenzial zu erwähnen sind. In diesem Artikel werden die molekularen und klinischen Effekte sowie die Neben- und Wechselwirkung von Milnacipran erläutert. Auch wird auf die Indikationsstellung für eine Therapie mit Milnacipran und mögliche Dosierungsschemen eingegangen. Abschließend werden Fallvignetten vorgestellt. Diese Arbeit stellt eine praxisnahe Darstellung über Pharmakologie und die Einsatzmöglichkeiten von Milnacipran für den Anwender dar.
Endovascular Treatment of Intracranial Atherosclerotic Stenosis
Authors: Nordmeyer Hannes , Chapot René , Haage Patrick 
Published: April 2019

Background Intracranial atherosclerotic stenosis (ICAS) causes 5 – 10 % of all ischemic strokes in the European population. Indication for endovascular treatment is a special challenge and the selection of material as well as interventional techniques essentially differs from the treatment of extracranial stenoses. According to recent studies patient selection became evidence based; however the method should not be abandoned. New technical approaches can contribute to avoid complications.
Method We performed a review of the literature with regard to conservative as well as endovascular treatment of ICAS. Different technical approaches are discussed and strategies to avoid complications are stressed. Based on the treatment indication, the positions of the authorities and the professional societies are taken into account.
Results and Conclusion A single self-expanding stent is approved for the treatment of ICAS. Balloon mounted and other self-expanding Stents are available for off-label use. Anatomical conditions and features of the stenosis determine the choice of material. Distal wire perforations causing intracranial bleedings may occur during exchange manoeuvres and constitute one of the technical complications in the treatment of ICAS. In contrast, there is hardly any efficient way to eliminate the risk of ischemia in the territory of perforating arteries arising from the intracranial posterior circulation and the middle cerebral artery. The results of the randomized prospective trials strengthen the conservative treatment of ICAS. Endovascular treatment should not be withheld from patients with either hemodynamic stenosis, recurrent ischemic events under best medical treatment in the territory of the stenosed vessel or acute occlusions of a stenosis.
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