bluuebunny/crossref_metadata_2025_split huggingface dataset:
Feasibility and safety of arthroscopic medial glenohumeral ligament and subscapularis tendon repair with knotless anchors: A cadaveric study in dogs
Authors: Penelas Alexandra , Gutbrod Andreas , Kühn Karolin , Pozzi Antonio 
Published: August 2018

AbstractObjectiveTo test the feasibility and safety of an arthroscopic imbrication of the medial glenohumeral ligament (MGHL) and the subscapularis tendon with knotless anchors in dogs.Study designCadaveric study.Sample populationTen limbs.MethodsCranial and caudal arms of the MGHL were imbricated with a 2.9‐ or a 2.4‐mm knotless anchor. A horizontal mattress suture secured with a 3.5‐mm knotless anchor was used to imbricate the subscapularis tendon. Computed tomography measurements included (1) the bone stock around the anchors, (2) the angle between the anchor and the joint surface (insertion angle), and (3) the angle formed by lines tangent to the cortices of the bones (safety angle). Limbs were dissected to assess the position of anchors. Safety and insertion angles and bone stock were compared among anchors with a Kruskal‐Wallis test (P < .05).ResultsSurgical repairs were achieved in all limbs, with only 2 of 30 anchors incorrectly placed, both in the glenoid. The safety angle of the humeral anchor (HA; median, 89°) was greater than that of the cranial glenoid anchor (CrGA; P = .0017) and the caudal glenoid anchor (CdGA; P < .001). The insertion angle of the HA (median, 68°) was also greater than that of the other anchors (P < .001 and P = .001). The insertion angle of the CrGA (median, 26°) was greater (P = .0191) than that of the CdGA (median, 7°). All anchors were inserted at the MGHL and subscapularis footprint.ConclusionArthroscopic imbrication of MGHL and subscapularis tendon was feasible. HA were safer to place than glenoid anchors.Clinical significanceThe results of this feasibility study justify further evaluation of the indications and outcomes of this technique in dogs with shoulder instability.
Sensors and signals: the role of reactive oxygen species in hypoxic pulmonary vasoconstriction
Authors: Smith Kimberly A. , Schumacker Paul T. 
Published: August 2018

AbstractWhen lung cells experience hypoxia, the functional response, termed hypoxic pulmonary vasoconstriction, activates a multitude of pathways with the goal of optimizing gas exchange. While previously controversial, overwhelming evidence now suggests that increased reactive oxygen species – produced at complex III of the mitochondrial electron transport chain and released into the intermembrane space – is the cellular oxygen signal responsible for triggering hypoxic pulmonary vasoconstriction. The increased reactive oxygen species (ROS) activate many downstream targets that ultimately lead to increased intracellular ionized calcium concentration and contraction of pulmonary arterial smooth muscle cells. While the specific targets of ROS signals are not completely understood, it is clear that this signalling pathway is critical for development and for normal lung function in newborns and adults.
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Operational
Time and Melt Fraction Based Optimization of a Phase Change Material Longitudinal Fin Heat
Sink
Authors: Jaya
    Krishna D. 
Published: August 2018

Abstract
In the present study, the numerical investigation has been performed for a phase change material (PCM)-based longitudinal fin heat sink. The fins are taken as an integral part of the heat sink and are made up of aluminum. The PCM considered in the study is RT44HC. Heat is transferred to the heat sink through its horizontal base. In order to simulate the melting behavior of the PCM, volume of fluid model has been used. To attain the best configuration with optimum operational time, Taguchi method has been used followed by analysis of melt fraction and maximum base temperature. The optimized heat sink configuration with maximum operational time has been obtained at the critical temperatures of 54.8 °C, 63 °C, and 72.6 °C.
Numerical Analysis of Conjugated Convection-Conduction Heat Transfer in a Microtube Gas Flow
Authors: Ramadan K. M. 
Published: August 2018

Abstract
Numerical solutions for conjugate heat transfer of a hydro-dynamically fully developed, thermally developing, steady, incompressible laminar gas flow in a microtube with uniform wall heat flux boundary condition are presented. The mathematical model takes into account effects of rarefaction, viscous dissipation, flow work, shear work, and axial conduction in both the wall and the fluid. The effect of the tube wall thickness, the wall-to-fluid thermal conductivity ratio, as well as other factors on heat transfer parameters is investigated, and comparisons with the case of zero wall thickness are presented as appropriate. The results illustrate the significance of heat conduction in the tube wall on convective heat transfer and disclose the significant deviation from those with no conjugated effects. Increasing the wall thickness lowers the local Nusselt number. Increasing the wall-to-fluid thermal conductivity ratio also results in lower Nusselt number. In relatively long and thick microtubes with high wall-to-fluid thermal conductivity ratio, the local Nusselt number exhibits minimum values in the entrance regions and at the end sections due to axial conduction effects. The analysis presented also demonstrate the significance of rarefaction, shear work, axial conduction, as well as the combined viscous dissipation and flow work effects on heat transfer parameters in a microtube gas flow. The combined flow work and viscous dissipation effects on heat transfer parameters are significant and result in a reduction in the Nusselt number. The shear work lowers the Nusselt number when heat is added to the fluid.
Developmental Dynamics of Long Noncoding RNA Expression during Sexual Fruiting Body Formation in Fusarium graminearum
Authors: Kim Wonyong , Miguel-Rojas Cristina , Wang Jie , Townsend Jeffrey P. , Trail Frances 
Published: August 2018

ABSTRACT

Long noncoding RNA (lncRNA) plays important roles in sexual development in eukaryotes. In filamentous fungi, however, little is known about the expression and roles of lncRNAs during fruiting body formation. By profiling developmental transcriptomes during the life cycle of the plant-pathogenic fungus
Fusarium graminearum
, we identified 547 lncRNAs whose expression was highly dynamic, with about 40% peaking at the meiotic stage. Many lncRNAs were found to be antisense to mRNAs, forming 300 sense-antisense pairs. Although small RNAs were produced from these overlapping loci, antisense lncRNAs appeared not to be involved in gene silencing pathways. Genome-wide analysis of small RNA clusters identified many silenced loci at the meiotic stage. However, we found transcriptionally active small RNA clusters, many of which were associated with lncRNAs. Also, we observed that many antisense lncRNAs and their respective sense transcripts were induced in parallel as the fruiting bodies matured. The nonsense-mediated decay (NMD) pathway is known to determine the fates of lncRNAs as well as mRNAs. Thus, we analyzed mutants defective in NMD and identified a subset of lncRNAs that were induced during sexual development but suppressed by NMD during vegetative growth. These results highlight the developmental stage-specific nature and functional potential of lncRNA expression in shaping the fungal fruiting bodies and provide fundamental resources for studying sexual stage-induced lncRNAs.

IMPORTANCE
Fusarium graminearum
is the causal agent of the head blight on our major staple crops, wheat and corn. The fruiting body formation on the host plants is indispensable for the disease cycle and epidemics. Long noncoding RNA (lncRNA) molecules are emerging as key regulatory components for sexual development in animals and plants. To date, however, there is a paucity of information on the roles of lncRNAs in fungal fruiting body formation. Here we characterized hundreds of lncRNAs that exhibited developmental stage-specific expression patterns during fruiting body formation. Also, we discovered that many lncRNAs were induced in parallel with their overlapping transcripts on the opposite DNA strand during sexual development. Finally, we found a subset of lncRNAs that were regulated by an RNA surveillance system during vegetative growth. This research provides fundamental genomic resources that will spur further investigations on lncRNAs that may play important roles in shaping fungal fruiting bodies.
Three-year outcomes after high hyperopia correction using photorefractive keratectomy with a large ablation zone
Authors: Kaluzny Bartlomiej J , Piotrowiak-Slupska Ilona , Kaszuba-Modrzejewska Magdalena , Stachura Joanna , Arba-Mosquera Samuel , Verma Shwetabh 
Published: August 2018

AimTo evaluate refractive and visual outcomes of photorefractive keratectomy (PRK) to treat high hyperopia using an aberration-neutral profile and large ablation zone.MethodsThis was a retrospective, consecutive observational case series at the Oftalmika Eye Hospital, Bydgoszcz, Poland. We included 51 consecutive eyes of 34 patients who underwent alcohol-assisted PRK to correct hyperopia within the range of +3.6 to +6.15 D (mean+4.61±0.67 D). Procedures were performed with an Amaris 750S excimer laser (Schwind eye-tech-solutions GmbH, Kleinostheim, Germany) using an aberration-neutral profile and a 10 mm total ablation zone. Refractive results, predictability, safety and efficacy were evaluated 3 years postoperatively.ResultsAt 1-year postsurgery, the mean manifest refraction spherical equivalent (MRSE) was −0.002±0.43 D and mean cylinder was −0.181±0.31 D, while the values were +0.09±0.46 D and −0.15±0.26 D, respectively, at 2 years (MRSE p<0.001) and +0.15±0.44 D and −0.15±0.26 D, respectively, at 3 years (MRSE p<0.001). 78% of eyes were within ±0.50 D of the attempted spherical equivalent correction. Three years postoperatively, 22% of eyes lost one line of corrected distance visual acuity and 27% gained a line or two. The change in the mean corneal spherical aberrations for the 6 mm zone was from 0.27±0.07 to 0.08±0.13 µm.ConclusionsHigh hyperopia correction with PRK using an aberration-neutral profile and large ablation zone provides good efficacy, safety, predictability and visual outcomes. Relatively low change of corneal spherical aberrations and low increase of hyperopia in the first three postoperative years were observed.
Prevalence of anaphylaxis among adults admitted to critical care for severe asthma exacerbation
Authors: Akenroye Ayobami T , Ajala Abayomi , Azimi-Nekoo Elham , de Vos Gabriele S 
Published: August 2018

BackgroundIn asthmatics, making a diagnosis of anaphylaxis could be challenging as respiratory symptoms are a common feature of both conditions. Identifying anaphylaxis is important to providing appropriate care. A prior study showed that anaphylaxis is sometimes misdiagnosed as acute asthma in children. We sought to identify the percentage of adults admitted to the intensive care unit (ICU) with asthma exacerbations who met criteria for anaphylaxis.MethodsRetrospective chart review of adults admitted with acute asthma to the ICU at Jacobi Medical Center, a Level 1 trauma centre in Bronx, New York. Study period was January 2012 to December 2014. Using the criteria outlined in the World Allergy Organization’s Anaphylaxis Guidelines, we identified patients who met criteria for anaphylaxis.Results105 patients were identified: 17 were excluded because their main reason(s) for admission was not asthma. 7 (8%) of the 88 cases eligible for the study met diagnostic criteria for anaphylaxis while 3 (3.4%) were highly likely to have had anaphylaxis. The baseline characteristics of the seven patients were similar to that of the overall population studied. They however had shorter onset of symptoms (median (IQR): 3.5 (2–6) vs 24 hours (12–72), p<0.001), were more likely to have been intubated (71%vs31%, p=0.04), received intramuscular epinephrine by the emergency medical services or in the emergency room (86%vs42%, p=0.04) and received antihistamines (28.6%vs1.2%, p=0.02). None of these seven patients died. Only one of the seven patients was prescribed an epinephrine pen injector and referred to an allergist at discharge.ConclusionIn this single-centre retrospective review, 3.4% of adults admitted to the ICU for acute severe asthma also met criteria for anaphylaxis.
A mixed methods study of the impact of consultant overnight working in an English Emergency Department
Authors: Penn Marion L , Monks Thomas , Pope Catherine , Clancy Mike 
Published: August 2018

BackgroundThere is a growing expectation that consultant-level doctors should be present within an ED overnight. However, there is a lack of robust evidence substantiating the impact on patient waiting times, safety or the workforce.ObjectivesTo evaluate the impact of consultant-level doctors overnight working in ED in a large university hospital.MethodsWe conducted a controlled interrupted time series analysis to study ED waiting times before and after the introduction of consultant night working. Adverse event reports (AER) were used as a surrogate for patient safety. We conducted interviews with medical and nursing staff to explore attitudes to night work.ResultsThe reduction seen in average time in department relative to the day, following the introduction of consultant was non-significant (−12 min; 95% CI −28 to 4, p=0.148). Analysis of hourly arrivals and departures indicated that overnight work was inherited from the day. There were three (0.9%) moderate and 0 severe AERs in 1 year. The workforce reported that night working had a negative impact on sleep patterns, performance and well-being and there were mixed views about the benefits of consultant night presence. Additional time off during the day acted as compensation for night work but resulted in reduced contact with ED teams.ConclusionsOur single-site study was unable to demonstrate a clinically important impact of consultant night working on total time patients spend in the department. Our analysis suggests there may be more potential to reduce total time in department during the day, at our study site. Negative impacts on well-being, and likely resistance to consultant night working should not be ignored. Further studies of night working are recommended to substantiate our results.
Eighty-two-year-old man with a systolic murmur
Authors: Kupsky Daniel F , Ananthasubramaniam Karthikeyan , Lee James 
Published: August 2018

Clinical IntroductionAn 82-year-old man with a history of coronary artery bypass surgery, hypertension and small bowel gastrointestinal stromal tumour underwent cardiac risk evaluation prior to surgical resection of his tumour. He was asymptomatic from a cardiovascular perspective, but his activity level was less than four metabolic equivalents. Physical examination was notable for a 2/6 systolic murmur at the apex. ECG showed sinus rhythm. A transthoracic echocardiogram was performed (figure 1 and online supplementary video 1).Supplementary file 1Figure 1Transthoracic echocardiography. (A) Mitral valve continuous wave Doppler and (B) tricuspid valve continuous wave Doppler.QUESTION:The findings in figure 1 are most likely due to which of the following?Atrioventricular conduction block.Acute severe aortic regurgitation.Patent ductus arteriosus.Atrial flutter.Severe mitral stenosis.
Testing messages about comparative risk of electronic cigarettes and combusted cigarettes
Authors: Yang Bo , Owusu Daniel , Popova Lucy 
Published: August 2018

IntroductionHealth agencies are grappling with communicating risks of electronic cigarettes (e-cigarettes) compared with combusted cigarettes. This study examined smokers’ responses to two types of comparative risk messages with one type incorporating more negative antismoking elements in the design.MethodsIn an online experiment, 1400 US adult (18+ years) current smokers and recent quitters were randomised to view one of three comparative risk messages about e-cigarettes (CR messages), one of three comparative risk messages that included more negative antismoking elements in the design (CR− messages) or a control message. Selection of outcomes was guided by the antismoking message impact framework. Multivariate analyses of covariance and logistic regression models analysed effects of messages on message evaluations, e-cigarette-related and cigarette-related beliefs and behavioural intentions.ResultsBoth CR and CR− messages decreased smokers’ intentions to smoke cigarettes, increased intentions to switch to e-cigarettes completely and increased perceptions that e-cigarettes are less harmful than combusted cigarettes. Neither message type increased dual use intentions relative to exclusive e-cigarettes use or smoking cessation. CR messages decreased perceived absolute risks of e-cigarettes and self-exempting beliefs about smoking, whereas CR− versus CR messages produced higher self-efficacy to quit smoking.ConclusionComparative risk communication might encourage smokers to switch to lower-harm tobacco products. Comparative risk messages with more negative antismoking elements in the design might be particularly effective, because they led to higher self-efficacy to quit smoking. Regulatory agencies may consider using comparative risk messages with more negative antismoking elements to educate the public about lower risk of e-cigarettes.
Skeletal muscle fiber characteristics in patients with chronic heart failure: impact of disease severity and relation with muscle oxygenation during exercise
Authors: Niemeijer Victor M. , Snijders Tim , Verdijk Lex B. , van Kranenburg Janneau , Groen Bart B. L. , Holwerda Andrew M. , Spee Ruud F. , Wijn Pieter F. F. , van Loon Luc J. C. , Kemps Hareld M. C. 
Published: August 2018

Skeletal muscle function in patients with heart failure and reduced ejection fraction (HFrEF) greatly determines exercise capacity. However, reports on skeletal muscle fiber dimensions, fiber capillarization, and their physiological importance are inconsistent. Twenty-five moderately impaired patients with HFrEF and 25 healthy control (HC) subjects underwent muscle biopsy sampling. Type I and type II muscle fiber characteristics were determined by immunohistochemistry. In patients with HFrEF, enzymatic oxidative capacity was assessed, and pulmonary oxygen uptake (V̇o2) and skeletal muscle oxygenation during maximal and moderate-intensity exercise were measured using near-infrared spectroscopy. While muscle fiber cross-sectional area (CSA) was not different between patients with HFrEF and HC, the percentage of type I fibers was higher in HC (46 ± 15 vs. 37 ± 12%, respectively, P = 0.041). Fiber type distribution and CSA were not different between patients in New York Heart Association (NYHA) class II and III. Type I muscle fiber capillarization was higher in HFrEF compared with HC[capillary-to-fiber perimeter exchange (CFPE) index: 5.70 ± 0.92 vs. 5.05 ± 0.82, respectively, P = 0.027]. Patients in NYHA class III had slower V̇o2 and muscle deoxygenation kinetics during onset of exercise and lower muscle oxidative capacity than those in class II ( P < 0.05). Also, fiber capillarization was lower but not compared with HC. Higher CFPE index was related to faster deoxygenation ( rspearman = −0.682, P = 0.001), however, not to muscle oxidative capacity ( r = −0.282, P = 0.216). Type I muscle fiber capillarization is higher in HFrEF compared with HC but not in patients with greater exercise impairment. Greater capillarization may positively affect V̇o2 kinetics by enhancing muscle oxygen diffusion. NEW & NOTEWORTHY The skeletal myopathy of chronic heart failure (HF) includes a greater percentage of fatigable type II fibers and, for less impaired patients, greater skeletal muscle fiber capillarization. Near-infrared spectroscopy measurements of skeletal muscle oxygenation indicate that greater capillarization may compensate for reduced blood flow in mild HF by enhancing the diffusive capacity of skeletal muscle. This thereby augments and speeds oxygen extraction during contractions, which is translated into faster pulmonary oxygen uptake kinetics.
Acclimatization of low altitude-bred deer mice (Peromyscus maniculatus) to high altitude
Authors: Dane D. Merrill , Cao Khoa , Lu Hua , Yilmaz Cuneyt , Dolan Jamie , Thaler Catherine D. , Ravikumar Priya , Hammond Kimberly A. , Hsia Connie C. W. 
Published: August 2018

A colony of deer mice subspecies ( Peromyscus maniculatus sonoriensis) native to high altitude (HA) has been maintained at sea level for 18–20 generations and remains genetically unchanged. To determine if these animals retain responsiveness to hypoxia, one group (9–11 wk old) was acclimated to HA (3,800 m) for 8 wk. Age-matched control animals were acclimated to a lower altitude (LA; 252 m). Maximal O2uptake (V̇o2max) was measured at the respective altitudes. On a separate day, lung volume, diffusing capacity for carbon monoxide (DLCO), and pulmonary blood flow were measured under anesthesia using a rebreathing technique at two inspired O2tensions. The HA-acclimated deer mice maintained a normal V̇o2maxrelative to LA baseline. Compared with LA control mice, antemortem lung volume was larger in HA mice in a manner dependent on alveolar O2tension. Systemic hematocrit, pulmonary blood flow, and standardized DLCOdid not differ significantly between groups. HA mice showed a higher postmortem alveolar-capillary hematocrit, larger alveolar ducts, and smaller distal conducting structures. In HA mice, absolute volumes of alveolar type I epithelia and endothelia were higher whereas that of interstitia was lower than in LA mice. These structural changes occurred without a net increase in whole-lung septal tissue-capillary volumes or surface areas. Thus, deer mice bred and raised to adulthood at LA retain phenotypic plasticity and adapt to HA without a decrement in V̇o2maxvia structural (enlarged airspaces, alveolar septal remodeling) and nonstructural (lung expansion under hypoxia) mechanisms and without an increase in systemic hematocrit or compensatory lung growth.NEW & NOTEWORTHY Deer mice ( Peromyscus maniculatus) are robust and very active mammals that are found across the North American continent. They are also highly adaptable to extreme environments. When introduced to high altitude they retain remarkable adaptive ability to the low-oxygen environment via lung expansion and remodeling of existing lung structure, thereby maintaining normal aerobic capacity without generating more red blood cells or additional lung tissue.