bluuebunny/crossref_metadata_2025_split huggingface dataset:
Competing endogenous RNA network crosstalk reveals novel molecular markers in colorectal cancer
Authors: Samir Nehal , Matboli Marwa , El‐Tayeb Hanaa , El‐Tawdi Ahmed , Hassan Mohmed K. , Waly Amr , EL‐Akkad Hesham A. E. , Ramadan Mohamed G. , Al‐Belkini Tarek N. , El‐Khamisy Sherif , El‐Asmar Farid 
Published: May 2018

AbstractThe competing endogenous RNA networks play a pivotal role in cancer diagnosis and progression. Novel properstrategies for early detection of colorectal cancer (CRC) are strongly needed. We investigated a novel CRC‐specific RNA‐based integrated competing endogenous network composed of lethal3 malignant brain tumor like1 (L3MBTL1) gene, long non‐coding intergenic RNA‐ (lncRNA RP11‐909B2.1) and homo sapiens microRNA‐595 (hsa‐miRNA‐595) using in silico data analysis. RT‐qPCR‐based validation of the network was achieved in serum of 70 patients with CRC, 40 patients with benign colorectal neoplasm, and 20 healthy controls. Moreover, in cancer tissues of 20 of the 70 CRC cases were involved in the study. The expression of RNA‐based biomarker network in both CRC and adjacent non‐tumor tissues and their correlation with the serum levels of this network members was investigated. Lastly, the expression levels of the chosen ceRNA was verified in CRC cell line. Our results revealed that the three RNAs‐based biomarker network (long non‐coding intergenic RNA‐[lncRNA RP11‐909B2.1], Homo sapiens microRNA‐595 [hsa‐miRNA‐595], and L3MBTL1 mRNA), had high sensitivity and specificity for discriminating CRC from healthy controls and also from benign colorectal neoplasm. The data suggest that among these three RNAs, serum lncRNA RP11‐909B2.1 could be a promising independent prognostic factors in CRC. The circulatory RNA based biomarker panel can act as potential biomarker for CRC diagnosis and prognosis.
Prednisone Pharmacokinetics During Pregnancy and Lactation
Authors: Ryu Rachel J. , Easterling Thomas R. , Caritis Steve N. , Venkataramanan Raman , Umans Jason G. , Ahmed Mahmoud S. , Clark Shannon , Kantrowitz‐Gordon Ira , Hays Karen , Bennett Brooke , Honaker Matthew T. , Thummel Kenneth E. , Shen Danny D. , Hebert Mary F. 
Published: May 2018

AbstractTo evaluate the steady‐state pharmacokinetics of prednisone and its metabolite prednisolone in pregnant and lactating female subjects, 19 subjects received prednisone (4‐40 mg/day orally) in early (n = 3), mid (n = 9), and late (n = 13) pregnancy as well as postpartum with (n = 2) and without (n = 5) lactation. Serial blood and urine samples were collected over 1 dosing interval. Prednisone and its metabolite, prednisolone, steady‐state noncompartmental pharmacokinetic parameters were estimated. During pregnancy, prednisone apparent oral clearance increased with dose (35.1 ± 11.4 L/h with 5 mg, 52.6 ± 5.2 L/h with 10 mg, and 64.3 ± 6.9 L/h with 20 mg, P = .001). Similarly, unbound prednisone apparent oral clearance increased with dose. In addition, prednisolone renal clearance increased with dose (0.3 ± 0.3 L/h with 5 mg, 0.5 ± 0.4 L/h with 10 mg, and 1.3 ± 1.1 L/h with 20 mg, P = .002). Higher prednisone (r = 0.57, P ≤ .05) and prednisolone (r = 0.75, P ≤ .05) concentrations led to a higher percentage of unbound drug. Breast‐milk/plasma area under the concentration‐time curve ratios were 0.5‐0.6 for prednisone and 0.02‐0.03 for prednisolone. Relative infant doses were 0.35% to 0.53% and 0.09% to 0.18%, for prednisone and prednisolone, respectively. Prednisone and prednisolone exhibit dose‐ and concentration‐dependent pharmacokinetics during pregnancy, and infant exposure to these agents via breast milk is minimal.
Changes in deciduous and permanent dentinal tubules diameter after several conditioning protocols: In vitro study
Authors: de los Angeles Moyaho‐Bernal María , Contreras‐Bulnes Rosalía , Rodríguez‐Vilchis Laura Emma , Rubio‐Rosas Efraín 
Published: May 2018

AbstractInnovators conditioning protocols are emerged in permanent dentin, however for deciduous dentin the information is limited; the aim of this study was to evaluate in vitro diameter of deciduous and permanent dentinal tubules after several conditioning protocols. Eighty dentin samples were distributed in sixteen groups (n = 5 p/g) and dentin surface was conditioned as follow: G1D/G1P acid etching; G2D/G2P, self‐etch adhesive; G3D/G3P, G4D/G4P, Er: YAG laser irradiation at 200 mJ‐25.5 J/cm2 and 300 mJ‐38.2 J/cm2, at 10 Hz under water spray respectively; G5D/G5P, G6D/G6P, G7D/G7P, and G8D/G8P were irradiated under the same energy densities followed phosphoric acid or self‐etch adhesive conditioning. The sample dentin of deciduous and permanent teeth was analyzed with scanning electron microscopy and tubule diameter was evaluated by Image Tools Scandium program. Data were subjected to one‐way analysis ANOVA to compare among groups with a level of significance at p ≤ .05. For deciduous dentin, diameters were from 1.52 ± 0.32 µm in G3D to 3.88 ± 0.37 µm in G1D; narrowest and widest diameter, respectively (p < .000). While permanent dentin tubules exhibited diameters from 1.16 ± 0.16/1.19 ± 0.12 µm in G7P/G8P to 2.76 ± 0.28 µm in G6P; narrowest and widest diameter, respectively (p < .000). All dentin conditioning protocols produced more open dentin tubules (diameter size) in deciduous dentin than permanent, specific conditioning protocols are required for each tissue (deciduous or permanent dentin), since same protocol produced stronger effects on primary dentin, which is important for dental clinical success in children and adolescents.
Gross morphology and scanning electron microscopy of the Bagrus Bayad (Forskal, 1775) oropharyngeal cavity with emphasis to teeth‐food adaptation
Authors: Alsafy Mohamed A. M. , Bassuoni Naglaa F. , Hanafy Basma G. 
Published: May 2018

AbstractBagrus Bayad (Forskal, 1775) is a bottom feeder fish feeds on different food items. Its oral cavity anatomy has many types of teeth adapted to the capacity and varieties of feeding. The aim of the present study was to use the gross anatomy and scanning electron microscopy to identify the anatomical characteristic, especially of the teeth and taste buds distributed on the roof and floor of the oropharyngeal cavity. The results clarified that Bagrus Bayad has two types of teeth; curved pointed and blunt teeth of different sizes. The teeth appeared on epithelial protrusions. The pharyngeal region had upper and lower pharyngeal teeth, the upper arranged into two oval prominences while the lower ones arranged into two triangular areas. The higher number of the pointed teeth is prove the carnivorous nature of the Bagrus Bayad. Three types of taste bud (I, II, & III) were found in the oropharyngeal cavity. The tongue present in the floor and free from teeth and characterized by the presence of taste bud type I and II. Many microridges of different arrangement observed on the surface epithelium of oropharyngeal cavity. The upper and lower valve characterized by the microridges, pores and taste buds. The knowledge of the nature of the oropharyngeal cavity of the Bagrus Bayad helps in identifying new and better methods for feeding in aquaculture.
Using multidimensional topological data analysis to identify traits of hip osteoarthritis
Authors: Rossi‐deVries Jasmine , Pedoia Valentina , Samaan Michael A. , Ferguson Adam R. , Souza Richard B. , Majumdar Sharmila 
Published: May 2018

BackgroundOsteoarthritis (OA) is a multifaceted disease with many variables affecting diagnosis and progression. Topological data analysis (TDA) is a state‐of‐the‐art big data analytics tool that can combine all variables into multidimensional space. TDA is used to simultaneously analyze imaging and gait analysis techniques.PurposeTo identify biochemical and biomechanical biomarkers able to classify different disease progression phenotypes in subjects with and without radiographic signs of hip OA.Study TypeLongitudinal study for comparison of progressive and nonprogressive subjects.PopulationIn all, 102 subjects with and without radiographic signs of hip osteoarthritis.Field Strength/Sequence3T, SPGR 3D MAPSS T1ρ/T2, intermediate‐weighted fat‐suppressed fast spin‐echo (FSE).AssessmentMultidimensional data analysis including cartilage composition, bone shape, Kellgren–Lawrence (KL) classification of osteoarthritis, scoring hip osteoarthritis with MRI (SHOMRI), hip disability and osteoarthritis outcome score (HOOS).Statistical TestsAnalysis done using TDA, Kolmogorov–Smirnov (KS) testing, and Benjamini‐Hochberg to rank P‐value results to correct for multiple comparisons.ResultsSubjects in the later stages of the disease had an increased SHOMRI score (P < 0.0001), increased KL (P = 0.0012), and older age (P < 0.0001). Subjects in the healthier group showed intact cartilage and less pain. Subjects found between these two groups had a range of symptoms. Analysis of this subgroup identified knee biomechanics (P < 0.0001) as an initial marker of the disease that is noticeable before the morphological progression and degeneration. Further analysis of an OA subgroup with femoroacetabular impingement (FAI) showed anterior labral tears to be the most significant marker (P = 0.0017) between those FAI subjects with and without OA symptoms.Data ConclusionThe data‐driven analysis obtained with TDA proposes new phenotypes of these subjects that partially overlap with the radiographic‐based classical disease status classification and also shows the potential for further examination of an early onset biomechanical intervention.Level of Evidence: 2Technical Efficacy: Stage 2J. Magn. Reson. Imaging 2018;48:1046–1058.
Blood perfusion of patellar bone measured by dynamic contrast‐enhanced MRI in patients with patellofemoral pain: A case–control study
Authors: van der Heijden Rianne A. , Poot Dirk H.J. , Ekinci Melek , Kotek Gyula , van Veldhoven Peter L.J. , Klein Stefan , Verhaar Jan A.N. , Krestin Gabriel P. , Bierma‐Zeinstra Sita M.A. , van Middelkoop Marienke , Oei Edwin H.G. 
Published: May 2018

BackgroundAltered perfusion might play an important role in the pathophysiology of patellofemoral pain (PFP), a common knee complaint with unclear pathophysiology.PurposeTo investigate differences in dynamic contrast‐enhanced (DCE)‐MRI perfusion parameters between patients with PFP and healthy control subjects.Population/Subjects/Phantom/Specimen/Animal ModelThirty‐five adult patients with PFP and 44 healthy adult control subjects.Field Strength/Sequence3T DCE‐MRI consisting of a sagittal, anterior‐posterior, frequency‐encoded, fat‐suppressed 3D spoiled gradient‐echo sequence with intravenous contrast administration.AssessmentPatellar bone volumes of interest (VOIs) were delineated by a blinded observer. Quantitative perfusion parameters (kep and ktrans) were calculated from motion‐compensated DCE‐MRI data by fitting Tofts' model. Weighted mean and unweighted median values of kep and ktrans were computed within the patellar bone VOIs.Statistical TestsDifferences in patellar bone perfusion parameters were compared between groups by linear regression analyses, adjusted for confounders.ResultsMean differences of weighted mean and unweighted median were 0.0039 (95% confidence interval [CI] –0.0013; 0.0091) and 0.0052 (95% CI –0.0078; 0.018) for ktrans, and 0.046 (95% CI –0.021; 0.11) and 0.069 (95% CI –0.017; 0.15) for kep, respectively.All perfusion parameters were not significantly different between groups (P‐values: 0.32; 0.47 for ktrans, and 0.24; 0.15) for kep. However, a significant difference in variance between populations was observed for ktrans (P‐value 0.007).Data ConclusionHigher patellar bone perfusion parameters were found in patients with PFP when compared to healthy control subjects, but these differences were not statistically significant. This result, and the observed significant difference in ktrans variance, warrant further research.Level of Evidence: 1Technical Efficacy: Stage 3J. Magn. Reson. Imaging 2018;47:1344–1350.
Diagnostic performance of intravoxel incoherent motion diffusion‐weighted imaging in the assessment of the dynamic status of myocardial perfusion
Authors: An Dong‐Aolei , Chen Bing‐Hua , Rui‐Wu  , Shi Ruo‐Yang , Bu Jun , Ge Heng , Hu Jiani , Xu Jian‐Rong , Wu Lian‐Ming 
Published: May 2018

BackgroundAcute myocardial infarction (AMI) is a major cause of morbidity and mortality worldwide and places a significant financial burden on our society.PurposeTo determine the feasibility of cardiac intravoxel incoherent motion (IVIM) in the consecutive evaluation of myocardial perfusion in myocardial infarction patients postpercutaneous coronary intervention (PCI) and to investigate the dynamic biological phenomena in myocardial perfusion after AMI.Study TypeProspective observational study.PopulationTwenty ST‐segment elevation myocardial infarction (STEMI) patients after reperfusion therapy and 12 healthy volunteers served as controls.Field Strength/SequenceCardiac MRI at 3T, including steady‐state free precession (SSFP) cine imaging, T2‐short time inversion recovery (T2‐STIR), late gadolinium enhancement (LGE), T2 mapping, and IVIM diffusion‐weighted imaging (DWI) were performed.AssessmentMyocardial T2 value and IVIM‐DWI‐associated parameters (ADCfast, ADCslow, and f value) of the infarcted myocardium at different timepoints, remote myocardium, and normal myocardium were analyzed by two experienced radiologists.Statistical TestsIndependent sample's t‐test, Pearson's, and Spearman's correlation and interobserver variability were applied. P ≤ 0.05 was considered significant.ResultsThe T2 value in ischemic myocardium measured on day 3 (73.58 ± 4.37 msec) was greater than at any other timepoint (24 hours, day 7, day 30; 66.66 ± 4.71 msec, 68.36 ± 4.18 msec, 64.98 ± 5.39 msec, respectively, P < 0.001). ADCfast and f values were significantly lower in ischemic myocardium than in the remote myocardium as well. The f value in ischemic myocardium at day 3 (0.0989 ± 0.02) was lower than at any other timepoint (24 hours, 7 day, 30 day; 0.1203 ± 0.02, 0.1109 ± 0.02, 0.1213 ± 0.02, respectively, P < 0.001.Data ConclusionThis preliminary study demonstrated that a dynamic process exists in the status of myocardial edema and myocardial perfusion in MI patients after PCI. The findings suggest myocardial perfusion would be best evaluated between day 3 and day 7.Level of Evidence: 2Technical Efficacy: Stage 2J. Magn. Reson. Imaging 2018;48:1602–1609
Effects of silymarin on metabolic syndrome: a review
Authors: Vahabzadeh Maryam , Amiri Nafise , Karimi Gholamreza 
Published: May 2018

AbstractMetabolic syndrome is one of the rising global health problems and medical challenges due to several clinical complications it may cause, for example increasing the risk of myocardial infarction and hypertension. However, great attention has been directed toward determining the worthiness of herbal medicines. There are emerging studies on preventive and therapeutic effects of silymarin on different components of metabolic syndrome. Extracted from the dried seeds of milk thistle plant (Silybum marianum L.), silymarin has been used in the treatment of different diseases for many years. Several protective effects have been identified for this herb such as decreasing insulin resistance, regulating blood pressure and lipid profile, as well as antioxidant and cytoprotective effects. This review aims to discuss available human and experimental researches into the promising effects of silymarin on different elements of metabolic syndrome. All related human and experimental papers published from 2012 to date were included in this review. Reviewing different human and experimental studies into the effects of silymarin on metabolic syndrome, we deduced that silymarin possesses promising effects on different components of this syndrome. Although the complete mechanism of action and target organs for silymarin require further verification and investigation, high‐risk individuals may benefit from supplementation with this herbal medicine. © 2018 Society of Chemical Industry
Diagnostic Performance of Transvaginal Ultrasound for Detecting Cervical Invasion In Women With Endometrial Carcinoma: A Systematic Review and Meta‐analysis
Authors: Alcázar Juan Luis , Pérez Lidia , Güell Olga , Haro Núria , Manzour Nabil , Chacon Enrique , Jurado Matías 
Published: May 2018

ObjectivesTo evaluate the role of transvaginal ultrasound (TVUS) for diagnosing cervical invasion in the preoperative assessment of endometrial carcinoma.MethodsA search for studies evaluating the role of TVUS for assessing cervical invasion in endometrial carcinoma from January 1990 to December 2016 was performed in the PubMed/MEDLINE, Web of Science, www.ClinicalTrials.gov, and www.who.int/trialsearchdatabases. The quality of the studies was evaluated by the Quality Assessment of Diagnostic Accuracy Studies 2.ResultsWe identified 211 citations. Ultimately, 17 studies comprising 1751 women were included. The mean prevalence of cervical invasion was 16.3%. The risk of bias was high in 7 studies for the domains “patient selection” and “index test,” whereas it was considered low for the “reference test” domain. Overall, the pooled estimated sensitivity, specificity, positive likelihood ratio, and negative likelihood ratio of TVUS for detecting cervical invasion were 63% (95% confidence interval [CI], 51%–74%), 91% (95% CI, 87%–94%), 10.2 (95% CI, 5.7–18.3), and 0.38 (95% CI, 0.28–0.53), respectively. Heterogeneity was high for both sensitivity and specificity.ConclusionsTransvaginal ultrasound has acceptable diagnostic performance for detecting cervical invasion in women with endometrial carcinoma.
Southern Kalahari piospheres: Looking beyond the sacrifice zone
Authors: Perkins Jeremy S. 
Published: May 2018

AbstractVegetation data collected around 8 piospheres in the southern Kalahari of Botswana show livestock impacts on vegetation cover and composition to be of limited spatial extent around the watering point. Dune crests can become active up to a distance of 1.2 km from the borehole with the latter sitting within an area of bare ground and a spectacular cauldron of active dunes some 50–200 m out. Data from an abandoned borehole show that the bare ground zone or “sacrifice zone” does recover, albeit with forbs and annuals, which serve to stabilise the formerly active dunes. Greater concern over the impact of piospheres lies beyond the sacrifice zone to the effects livestock keeping has had upon the resilience of the Kalahari ecosystem and the creation of large tracts of “empty Savannah”. Livestock expansion has acted to block migratory corridors that link the protected areas and in turn trigger largely reactive wildlife management measures. These include fencing and Artificial Waterpoint Provision in an attempt to redress spiralling human wildlife conflict and the isolation of wild ungulates from historical sources of surface water, particularly in a drought. Successive aerial surveys of Kalahari ungulates show the failure of migratory ungulates such as blue wildebeest and red hartebeest to recover from the drastic die‐offs that occurred in the 1980s drought. The need to manage the Kalahari ecosystem as a whole to ensure coexistence between domestic and wild ungulate populations and a greater diversity of livelihood opportunities to the benefit of impoverished rural communities is emphasised.
Whole body and local hyperthermia enhances the photosensitizing efficacy of 3‐[(1′‐hexyloxy)ethyl]‐3‐Devinylpyropheophorbide‐a (HPPH)
Authors: Srivatsan Avinash , Sen Arindam , Cheruku Ravindra R. , Missert Joseph R. , Durrani Farukh A. , Guru Khurshid , Pandey Ravindra K. 
Published: May 2018

Background and ObjectivesIn this study, we evaluated the impact of hyperthermia in photosensitizing efficacy of 3‐[(1′‐hexyloxy)ethyl‐3‐devinylpyropheophorbide‐a (HPPH or Photochlor) for the treatment of cancer by photodynamic therapy (PDT).Study Design/Materials and MethodsThe outcome of both whole body hyperthermia (WBH) and local hyperthermia (LH) in combination with HPPH‐PDT was determined in BALB/c and nude mice bearing Colon26 and U87 tumors, respectively. LH was performed by using an indigenously designed heating device, that was heated to the required temperature using a circulating water bath. The device which has flexible membrane on one side was placed on skin above the tumor. The temperature of the tumor was monitored using a thermocouple sensor placed on the surface of the tumor capable of measuring the temperature within 0.1°C. Uptake of the photosensitizer in tumors was determined by fluorescence using an IVIS or a Nuance Imaging System. The PDT was performed by exposing the tumors to 665 nm laser loght, (135 J/cm2, 75 mW/cm2) at the maximal uptake time of HPPH. Tumor size was measured daily using vernier calipers.ResultsThe improved PDT efficacy (long‐term percentage tumor cure) in combination with hyperthermia is possible due to an increase in tumor‐uptake of the photosensitizer (PS), confirmed by in vivo fluorescence imaging and also by increased tumor perfusion and decreased hypoxia as have been reported previously (Sen et al. [2011] Cancer Res. 71:3872‐3880 In Vivo. 20:689‐695). Interestingly, compared to whole body hyperthermia, the 14C‐ HPPH biodistribution data under local hyperthermia showed similar tumor‐uptake in BALB/c mice bearing Colon26 tumors, but significantly lower uptake in other organs and in the blood.ConclusionOur study demonstrates that both, fever range whole body and local hyperthermia in combination with HPPH‐PDT enhances the long‐term tumor cure of BALB/c and nude mice implanted with Colon26 and U87 tumors respectively. Lasers Surg. Med. 50:506–512, 2018. © 2018 Wiley Periodicals, Inc.
5‐ALA in the management of malignant glioma
Authors: Stepp Herbert , Stummer Walter 
Published: May 2018

BackgroundPatients suffering from malignant gliomas have a poor prognosis. For the surgical treatment of these tumors, 5‐aminolevulinic acid (5‐ALA) has become a new standard.AimsThis review intends to provide an overview over current status, significance, limitations, and future perspectives of 5‐ALA based fluorescence guided surgery and photodynamic therapy for brain tumor patients.Materials and MethodsFrom peer reviewed publications on the many aspects connected with this topic, those with potential clinical relevance were selected and put in the context of our own experience.Results and DiscussionThe high tumor selectivity of accumulation of fluorescent protoporphyrin IX (PpIX) after systemic administration of 5‐ALA enables intra‐operative fluorescence guidance, which is unimpaired by brainshift and does not require expensive equipment. The neurosurgical aim of complete resection of enhancing tumor can now more easily be achieved, which improves prognosis in these patients. Nevertheless, despite better surgery tumors will inevitably recur. In order to further prolong survival, the phototoxic properties of PpIX are presently being exploited in clinical trials of post‐operative or interstitial photodynamic therapy (PDT).Conclusion5‐ALA based fluorescence guidance and PDT offer an intriguing new option for the management of malignant gliomas. Lasers Surg. Med. 50:399–419, 2018. © 2018 The Authors. Lasers in Surgery and Medicine Published by Wiley Periodicals LLC