bluuebunny/crossref_metadata_2025_split huggingface dataset:
Research on the influence of radiotherapy-related genes on immune infiltration, immunotherapy response and prognosis in melanoma based on multi-omics
Authors: Shi Yujing , Zhao Wantong , Ding Yuanjian , Ge Xiaolin , Ju Mengyang 
Published: December 2024

BackgroundSkin cutaneous melanoma (SKCM) is a significant oncological challenge due to its aggressive nature and poor treatment outcomes. This study explores the comprehensive effects of radiotherapy (RT) in SKCM, focusing on cell signaling pathways, immune infiltration, immune gene correlations, immunotherapy response, and prognosis.MethodsUsing the Cancer Genome Atlas (TCGA) database, differentially expressed genes (DEGs) in SKCM patients undergoing RT were identified. A risk score model based on these DEGs was developed to assess the effects of RT-related genes on drug sensitivity, immune cell infiltration, immunotherapy response, and prognosis through multi-omics analysis. Human melanoma cells UACC62 and UACC257 were irradiated with 8 Gy gamma ray to establish an in vitro model, verifying the impact of radiotherapy on gene expression.ResultsThe risk score demonstrated significant prognostic value and emerged as an independent prognostic factor. miRNA-mRNA and transcription factor regulatory networks underscored its clinical significance. Four key genes were identified: DUSP1, CXCL13, SLAMF7, and EVI2B. Analysis of single-cell and immunotherapy datasets indicated that these genes enhance immune response and immunotherapy efficacy in melanoma patients. PCR results confirmed that gamma rays increased the expression of these genes in human melanoma cells UACC62 and UACC257.ConclusionUsing a multi-omics approach, we analyzed and validated the impact of RT on the immune landscape of melanoma patients. Our findings highlight the critical role of RT-related genes in predicting SKCM prognosis and guiding personalized therapy strategies, particularly in the context of immunotherapy. These contribute to understanding the role of radiotherapy combined with immunotherapy in melanoma.
Comparison of antifungal drugs in the treatment of invasive pulmonary aspergillosis: a systematic review and network meta-analysis
Authors: Cheng Jing , Han Hedong , Kang Wenwen , Cai Zijin , Zhan Ping , Lv Tangfeng 
Published: December 2024

BackgroundVoriconazole, isavuconazole, and amphotericin (AmB) formulations are currently recommended to treat invasive pulmonary aspergillosis (IPA). We aimed to estimate the efficacy of different antifungal drugs in the initial treatment of IPA.MethodsWe included all available randomized controlled trials (RCTs) evaluating first-line treatments for IPA by searching PubMed, Medline, EMBASE, the Cochrane Library, and the ClinicalTrials.gov database. We performed a network meta-analysis to compare the relative efficacy of different drugs in treating IPA. The primary outcomes were the overall response and all-cause mortality (ACM).ResultsEight studies were identified that compared different drugs including voriconazole, isavuconazole, posaconazole, anidulafungin, liposomal AmB (L-AmB) at standard, high and low doses (3-5 mg/kg/d; 10 mg/kg/d; 1 mg/kg/d), AmB deoxycholate (dAmB) and amphotericin B colloidal dispersion (ABCD). We found that second-generation triazole antifungal drugs containing voriconazole, isavuconazole, and posaconazole exhibited significantly superior overall response to dAmB and ABCD. Voriconazole was ranked as the best drug on network rank analysis. We found no difference in efficacy between triazole antifungals and L-AmB. A combination of voriconazole with anidulafungin, isavuconazole and voriconazole showed significantly better safety than dAmB.ConclusionThe efficacy of second-generation triazole antifungal drugs for the first-line treatment of IPA is comparable with L-AmB and is better than both dAmB and ABCD. Isavuconazole may show better safety than voriconazole and posaconazole. Combination therapy with voriconazole and anidulafungin may serve as an alternative option for IPA patients with limited drug tolerance.Systematic review registrationhttps://inplasy.com/.
Association between chronic pain and cognitive frailty among middle-aged and elderly individuals: evidence from the China Health and Retirement Longitudinal Study
Authors: Li Tianjiao , Li Lingxuan , Xie Hongyang , Ping Rongyu , Guo Yane , Li Dongmei , Zhang Yuwei , Bai Xiujuan , Sun Bo 
Published: December 2024

BackgroundFrailty, particularly cognitive frailty, is an escalating public health issue. Cognitive frailty is defined by the simultaneous presence of physical frailty and cognitive impairment, without a confirmed diagnosis of dementia, and has become a significant geriatric syndrome. This study aimed to explore the association between chronic pain and the risk of cognitive frailty.MethodsWe utilized data from two waves (2011 and 2015) of the China Health and Retirement Longitudinal Study (CHARLS), conducting both cross-sectional and longitudinal analyses involving 17,705 Chinese adults aged 45 years and older. Chronic pain was defined as pain reported at both time points. Cognitive function was evaluated using a questionnaire adapted from the Telephone Interview for Cognitive Status. The frailty index (FI) was derived from a 30-item assessment. Cognitive frailty was characterized by the co-occurrence of cognitive impairment and physical frailty.ResultsAmong the 14,285 participants, 5.39% exhibited cognitive frailty at baseline. Both cross-sectional and longitudinal analyses indicated that individuals suffering from chronic pain faced a higher likelihood of developing cognitive frailty compared to those without pain. After adjusting for potential confounders, multivariate models also indicated a higher odds of cognitive frailty for participants with chronic pain.ConclusionChronic pain is significantly associated with an elevated risk of cognitive frailty among middle-aged and elderly individuals. These findings highlight the importance of managing chronic pain to mitigate the risk of cognitive frailty, thereby potentially enhancing the quality of life for the aging population and alleviating the economic burden on families and society.
Probing the diagnostic values of plasma cf-nDNA and cf-mtDNA for Parkinson’s disease and multiple system atrophy
Authors: Ying Chao , Li Yuan , Zhang Hui , Pang Shimin , Hao Shuwen , Hu Songnian , Zhao Lifang 
Published: December 2024

BackgroundCell loss and mitochondrial dysfunction are key pathological features of idiopathic Parkinson’s disease (PD) and multiple system atrophy (MSA). It remains unclear whether disease-specific changes in plasma circulating cell-free nuclear DNA (cf-nDNA) and mitochondrial DNA (cf-mtDNA) occur in patients with PD and MSA. In this study, we investigated whether plasma cf-nDNA, cf-mtDNA levels, as well as cf-mtDNA integrity, are altered in patients with PD and MSA.MethodsTaqMan probe-based quantitative PCR was employed to measure plasma cf-nDNA levels, cf-mtDNA copy numbers, and cf-mtDNA deletion levels in 171 participants, including 76 normal controls (NC), 62 PD patients, and 33 MSA patients. A generalized linear model was constructed to analyze differences in circulating cell-free DNA (cfDNA) biomarkers across clinical groups, while a logistic regression model was applied to assess the predictive values of these biomarkers for developing PD or MSA. Spearman correlations were used to explore associations between the three cfDNA biomarkers, demographic data, and clinical scales.ResultsNo significant differences in plasma cf-nDNA levels, cf-mtDNA copy numbers, or cf-mtDNA deletion levels were observed among the PD, MSA, and NC groups (all P &gt; 0.05). Additionally, these measures were not associated with the risk of developing PD or MSA. In PD patients, cf-nDNA levels were positively correlated with Hamilton Anxiety Rating Scale scores (Rho = 0.382, FDR adjusted P = 0.027). In MSA patients, cf-nDNA levels were positively correlated with International Cooperative Ataxia Rating Scale scores (Rho = 0.588, FDR adjusted P = 0.011) and negatively correlated with Montreal Cognitive Assessment scores (Rho = −0.484, FDR adjusted P = 0.044). Subgroup analysis showed that PD patients with constipation had significantly lower plasma cf-mtDNA copy numbers than those without constipation (P = 0.049). MSA patients with cognitive impairment had significantly higher cf-nDNA levels compared to those without (P = 0.008).ConclusionPlasma cf-nDNA level, cf-mtDNA copy number, and cf-mtDNA deletion level have limited roles as diagnostic biomarkers for PD and MSA. However, their correlations with clinical symptoms support the hypothesis that cell loss and mitochondrial dysfunction are involved in PD and MSA development.
Combining dosiomics and machine learning methods for predicting severe cardiac diseases in childhood cancer survivors: the French Childhood Cancer Survivor Study
Authors: Bentriou Mahmoud , Letort Véronique , Chounta Stefania , Fresneau Brice , Do Duyen , Haddy Nadia , Diallo Ibrahima , Journy Neige , Zidane Monia , Charrier Thibaud , Aba Naila , Ducos Claire , Zossou Vincent S. , de Vathaire Florent , Allodji Rodrigue S. , Lemler Sarah 
Published: December 2024

BackgroundCardiac disease (CD) is a primary long-term diagnosed pathology among childhood cancer survivors. Dosiomics (radiomics extracted from the dose distribution) have received attention in the past few years to assess better the induced risk of radiotherapy (RT) than standard dosimetric features such as dose-volume indicators. Hence, using the spatial information contained in the dosiomics features with machine learning methods may improve the prediction of CD.MethodsWe considered the 7670 5-year survivors of the French Childhood Cancer Survivors Study (FCCSS). Dose-volume and dosiomics features are extracted from the radiation dose distribution of 3943 patients treated with RT. Survival analysis is performed considering several groups of features and several models [Cox Proportional Hazard with Lasso penalty, Cox with Bootstrap Lasso selection, Random Survival Forests (RSF)]. We establish the performance of dosiomics compared to baseline models by estimating C-index and Integrated Brier Score (IBS) metrics with 5-fold stratified cross-validation and compare their time-dependent error curves.ResultsAn RSF model adjusted on the first-order dosiomics predictors extracted from the whole heart performed best regarding the C-index (0.792 ± 0.049), and an RSF model adjusted on the first-order dosiomics predictors extracted from the heart’s subparts performed best regarding the IBS (0.069 ± 0.05). However, the difference is not statistically significant with the standard models (C-index of Cox PH adjusted on dose-volume indicators: 0.791 ± 0.044; IBS of Cox PH adjusted on the mean dose to the heart: 0.074 ± 0.056).ConclusionIn this study, dosiomics models have slightly better performance metrics but they do not outperform the standard models significantly. Quantiles of the dose distribution may contain enough information to estimate the risk of late radio-induced high-grade CD in childhood cancer survivors.
Metabolomics and network pharmacology–guided analysis of TNF-α expression by Argemone mexicana (Linn) targeting NF-kB the signalling pathway in cancer cell lines
Authors: Kulshrestha Sunanda , Goel Anjana , Banerjee Subhadip , Sharma Rohit , Khan Mohammad Rashid , Chen Kow-Tong 
Published: December 2024

IntroductionCancer has emerged as one of the leading causes of fatality all over the world. Phytoconstituents are being studied for their synergistic effects, which include disease prevention by altering molecular pathways and immunomodulation without side effects. The present experiment aims to explore the cancer preventive activities of Argemone mexicana Linn leaves extract in skin cancer cell lines (A431) and colon cancer cell lines (COLO 320DM)). In addition, TNF-α expression patterns and NF-kB signaling pathways have been examined.MethodsLC/MS study of Argemone mexicana Linn extracts in various solvents revealed anti-cancerous phytoconstituents. Network pharmacology analysis used Binding DB, STRING, DAVID, and KEGG for data mining to evaluate predicted compounds using functional annotation analysis. Cytoscape 3.2.1 created “neighbourhood approach” and networks. The MNTD of these extracts was tested on L929 fibroblasts. Skin cancer (A431) and colon cancer (COLO 320DM) cell lines were tested for IC50 inhibition. Evaluation of TNF-α and NF-kB expression in cell culture supernatants and homogenates reveals anti-cancerous effects.ResultsLC-MS analysis of extracts predicted the presence of anticancer alkaloids Berberine, Atropine, Argemexicin, and Argemonin. In Network pharmacology analysis, enrichment was linked to the PI3-AKT pathway for both cancer types. MNTD was calculated at 1000μg/ml in L929. The ethanolic extract at 1000μg/ml significantly inhibited skin cancer cell proliferation by 67% and colon cancer cells by 75%. Ethanolic extract significantly reduced TNF-α expression in both cell lines (p&lt;0.001), with the highest inhibition at 1000μg/ml. In TNF-α stimulated cell lines, 1000μg/ml ethanolic extract significantly reduced the regulation of the NF-kB pathway, which plays a role in cancer progression (p&lt;0.001).ConclusionArgemone mexicana Linn. known as ‘swarnkshiri’ in Ayurveda has been reported to be used by the traditional healers for the treatment of psoriasis and its anti-inflammatory and anti-cancerous properties, according to the Indian Medicinal Plant dictionary. In the experiment, the abatement in the expression of inflammatory cytokine TNF-α and inhibition of NF-kB transcription factor activation could be linked with the downregulation of cancer cell proliferation. The study revealed the anticancer activity of Argemone mexicana Linn in the cancer cell lines and paved a pathway for molecular approaches that could be explored more in In vivo studies.
Comparative transcriptomics of a generalist aphid, Myzus persicae and a specialist aphid, Lipaphis erysimi reveals molecular signatures associated with diversity of their feeding behaviour and other attributes
Authors: Sharma Manvi , Oraon Praveen Kumar , Srivastava Rakesh , Chongtham Rubina , Goel Shailendra , Agarwal Manu , Jagannath Arun 
Published: December 2024

IntroductionAphids are phloem sap-sucking insects and are a serious destructive pest of several crop plants. Aphids are categorized as “generalists” or “specialists” depending on their host range. Myzus persicae (Sulz.) is a generalist aphid with a broad host range while Lipaphis erysimi (Kalt.), a specialist aphid, has a narrow host range. Aphid infestation involves several sequential stages including host recognition and selection, overcoming primary plant defence barriers, feeding on phloem sap and detoxification of host defence responses. Information on the molecular basis of variations between generalist and specialist aphids with reference to the above processes is limited.MethodsIn the current study, we generated transcriptome data of M. persicae and L. erysimi from adult and nymph stages and analysed the differential expression of genes between adults of the generalist and specialist aphid and similarly, between nymphs of the two aphid species. We categorized these differentially expressed genes into nine different categories namely, chemosensation-related, plant cell wall degrading enzymes, detoxification-related, digestive enzymes, peptidases, carbohydrate-, lipid-, amino acid-metabolism and reproduction. We also identified putative effector molecules in both M. persicae and L. erysimi from the transcriptome data,Results and discussionGene expression analysis identified 7688 and 8194 differentially expressed unigenes at adult and nymph stages, respectively of M. persicae and L. erysimi. M. persicae showed significantly higher levels of expression in a greater number of unigenes (5112 in adults and 5880 in nymphs) in contrast to the specialist, L. erysimi (2576 in adults and 2314 in nymphs) in both developmental stages. In addition, M. persicae displayed a greater number (350 in adults and 331 in nymphs) of upregulated unigenes involved in important processes such as host recognition, plant cell wall degradation, detoxification, digestion and metabolism, which correlate with its dynamic and polyphagous nature in contrast to the specialist (337 in adults and 251 in nymphs). We also observed a greater number of putative effectors in M. persicae (948 in adults and 283 in nymphs) than L. erysimi (797 in adults and 245 in nymphs). Based on our analysis, we conclude that the generalist aphid, M. persicae has a more diversified and stronger arsenal of genes that influence its polyphagous feeding behaviour and effective response to plant defence mechanisms against insect-herbivory. Our study provides a compendium of such candidate genes that would be most useful in studies on aphid biology, evolution and control.
Acupuncture and related therapies for anxiety and depression in patients with premature ovarian insufficiency and diminished ovarian reserve: a systematic review and meta-analysis
Authors: Huang Sidan , Zhang Danni , Shi Xuliang , Zhang Yi , Wang Xuesong , She Yanfen , Liang Ce , Li Xinyue , Zaslawski Christopher 
Published: December 2024

BackgroundThe decreased ovarian function has a negative impact on the mental health of women and increases the risk of anxiety and depression. A growing number of clinical studies have demonstrated that acupuncture-related therapies can effectively and safely restore hormone levels and improve ovarian reserve function. However, the effectiveness of acupuncture-related therapies in alleviating anxiety and depression symptoms in patients with ovarian hypofunction has not been thoroughly evaluated. Therefore, this study conducted a systematic review and meta-analysis to assess the impact of the different acupuncture-related therapies on the mental health of patients with ovarian hypofunction.MethodsWe comprehensively searched eight famous databases for randomized controlled trials up to October 30, 2024. Databases include PubMed, Web of Science, EMBASE and Cochrane Library, China Biomedical (CBM), China National Knowledge Infrastructure (CNKI), Wanfang Database and VIP Database.ResultsThe study included 12 RCTs, involving 780 patients with ovarian hypofunction, including 403 patients with POI, 297 patients with DOR, and 80 patients with POF. Acupuncture-related therapy was obviously superior to hormone therapy in relieving anxiety symptoms (SMD: -0.90; 95%CI: -1.28, -0.53; P&lt;0.000 01) and depressive symptoms (SMD: -0.82; 95% CI: -1.25, -0.40; P=0.0001).ConclusionsAcupuncture-related therapy was more effective than hormone therapy in improving anxiety and depression symptoms in patients with ovarian hypofunction. This study supports the use of acupuncture-related therapies for women experiencing decreased ovarian function associated with mental health issues.Systematic review registrationhttps://www.crd.york.ac.uk/, identifier CRD42023488015.
Willingness to pay for improved solid waste management and associated factors among households in Debre Berhan town, North Shoa Zone, Amhara, Ethiopia, 2022
Authors: Chernet Dawit , Sema Wondimu , Gebeyehu Amare , Wogayehu Belachew Tekleyohannes 
Published: December 2024

BackgroundThe willingness to pay for better solid waste management serves as a framework that allows households to contribute a maximum amount of money to service improvement.ObjectivesThis study determined the willingness to pay for improved solid waste management and the associated factors among households in Debre Berhan town, Ethiopia, in 2022.MethodsA community-based cross-sectional study was conducted in Debre Berhan town, from June to July 2022. Multistage sampling techniques were employed to select household heads using semi-structured and pre-tested questionnaires. Data were entered using EpiData version 4.6 and analyzed using Stata version 14. The multivariable Tobit regression model was built upon a univariable Tobit regression framework. The relationship between dependent and independent variables was declared statistically significant in the final model at a p-value of ≤0.05 in the 95% confidence interval.ResultsThis study included 518 households, with a response rate of 99.23%. A total of 472 households 91.1% (95% CI: 88.1–93.5) reported their willingness to pay for improved solid waste management. The average monthly willingness to pay was 54.83 Ethiopian Birr. The amount of solid waste generated (β: 10.49; 95% CI: 2.85–18.12), knowledge (β: 7.33; 95% CI: 2.43–12.22), perception (β: 10.01; (95% CI: 5.83–14.19), monthly income (β: 13.23; 95% CI: 6.20–20.26), education level (β: 14.30; 95% CI: 3.48–13.63), house ownership (β: 8.56; 95% CI: 3.48–13.63), family size (β: 3.88; 95% CI: 2.01–5.74), and being a female household head (β: 5.73; 95% CI: 1.73–9.70) were the significantly associated factors.ConclusionWillingness to pay for improved solid waste management services was high. The town municipality should take advantage of this willingness to pay by installing an improved solid waste management, which can help alleviate budget constraints through active household participation in funding these services.
Arquitetura e Saudabilidade em Clínica de Imaginologia: análise do impacto do espaço interno na saúde de seus ocupantes
Authors: Maksoud Lara Bezerra , Pirró Lúcia Fernanda de Souza 
Published: December 2024

Ambientes clínicos auxiliares, como de imaginologia, costumam ser pouco difundidos em pesquisas relativas à qualidade arquitetônica em hospitais e o impacto de seus espaços sobre seus ocupantes. As pesquisas exibidas comumente se atentam às áreas de atendimento imediato, como Unidades de Urgência e Emergência (UUE), Unidades de Terapia Intensivas (UTI) e alas para recuperação. A reflexão acerca da humanização ambiental em projetos hospitalares, reforça a necessidade de estímulos ambientais que influenciem positivamente seus ocupantes, aprimorando a saúde e bem-estar. O objetivo da presente pesquisa foi analisar ambientes da subunidade de ressonância magnética de uma clínica de imaginologia, aferindo os impactos dos ambientes averiguados na saúde de seus usuários e contribuindo para reflexões acima do tema abordado. O trabalho se baseou em revisão literária (levantamento de dados secundários) e um estudo de caso, de uma clínica de imaginologia privada, onde foram aplicados métodos de avaliação pós-ocupação (APO), como walkthrough[1] e levantamento espacial (levantamento de dados primários). Como resultados, são apontados os principais problemas provenientes de espaços com má qualidade ambiental e os impactos sobre a saúde de seus usuários. Ao fim, são apresentadas alternativas, de baixo custo e fácil implantação, capazes de enriquecer o ambiente e aprimorar a saúde dos ocupantes.

[1] Método de análise que consiste na observação in loco, para averiguar e identificar os principais aspectos existentes no ambiente averiguado.
NGHIÊN CỨU TÍNH CHẤT Y SINH CỦA MÀNG SrTiO3:Er3+/ Yb3+ LÊN TITAN ĐỊNH HƯỚNG ỨNG DỤNG TRONG CẤY GHÉP Y SINH
Authors: Tuyền Nguyễn Thị Thanh , Anh Nguyễn Thị Mai , Hà Lê Tiến , Tuấn Tạ Quốc , Hướng Trần Văn , Vượng Phạm Hùng 
Published: December 2024

Trong nghiên cứu này, màng SrTiO3:Er3+ /Yb3+ phủ lên titan được chế tạo thành công bằng phương pháp thủy nhiệt ở 200°C cho mục đích cấy ghép y sinh. Các ion đất hiếm Er3+ và Yb3+ được đồng pha tạp vào màng nền SrTiO3 bằng cách cố định nồng độ Er3+ và thay đổi nồng độ Yb3+. Cấu trúc và tính chất của màng được đặc trưng bởi nhiễu xạ tia X (XRD), và ảnh hiển vi điện tử quét (SEM). Lớp phủ của vật liệu có cấu trúc perovskite được xử lý ở nhiệt độ 800°C. Vật liệu hình thành các màng vảy có kích thước khá đồng đều, có chiều dày thành vảy khoảng từ 2 - 5 nm và kích thước vảy khoảng 3 -6 μm. Tính chất bề mặt được khảo sát bằng phương pháp đo góc thấm ướt với vật liệu SrTiO3:Er3+ /Yb3+ có khả năng thấm ướt cao hơn vượt trội so với Ti. Kính hiển vi laser quét hội tụ đồng tiêu (CLSM) được sử dụng để kiểm tra trạng thái phân bố của tế bào và xác nhận tế bào thận chuột bám dính tốt trên mẫu thử nghiệm sau 48h nuôi cấy. Kết quả chỉ ra rằng, lớp màng SrTiO3:Er3+ /Yb3+ có tính chất y sinh tốt phù hợp cho mục đích ứng dụng làm vật liệu cấy ghép y sinh.