bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mechanical Stimuli Affect E. Coli Heat Stable Enterotoxin (ST)-Cyclic GMP Signaling in a Human Enteroid Intestine-Chip Diarrhea Model
Authors: Sunuwar Laxmi , Yin Jianyi , Kasendra Magdalena , Karalis Katia , Kaper James , Fleckenstein James , Donowitz Mark 
Published: September 2019

ABSTRACTModeling host-pathogen interactions with human intestinal epithelia using enteroid monolayers on permeable supports (such as Transwells) represents an alternative to animal studies or use of colon cancer-derived cell lines. However, the static monolayer model does not expose epithelial cells to mechanical forces normally present in the intestine, including luminal flow and serosal blood flow (shear force) or peristaltic forces. To determine the contribution of mechanical forces in the functional response of human small intestine to a pathogen virulence factor, human jejunal enteroids were cultured as monolayers in microengineered fluidic-based Organ-Chips (Intestine-Chips), exposed to enterotoxigenic E. coli heat-stable enterotoxin A (ST), and evaluated under conditions of static fluid, apical and basolateral flow, and flow plus repetitive stretch. Application of flow increased epithelial cell height, transcription of the cyclic nucleotide transporting protein MRP4, and apical and basolateral secretion of cGMP under baseline, unstimulated conditions. Addition of ST under flow conditions increased apical and basolateral secretion of cGMP relative to static conditions, but did not enhance intracellular cGMP accumulation. Cyclic stretch did not have any significant effect beyond that contributed by flow. This study demonstrates that fluid flow application initiates changes in intestinal epithelial cell characteristics relative to static culture conditions under both baseline conditions and with exposure to ST enterotoxin, and suggests that further investigations of application of these mechanical forces will provide insights into physiology and pathophysiology that more closely resembles intact intestine than study under static conditions.
Cysteine crosslinking in native membranes establishes the transmembrane architecture of Ire1
Authors: Vaeth Kristina , Mattes Carsten , Reinhard John , Covino Roberto , Stumpf Heike , Hummer Gerhard , Ernst Robert 
Published: September 2019

AbstractThe endoplasmic reticulum (ER) is a key organelle of membrane biogenesis and crucial for the folding of both membrane and secretory proteins. Sensors of the unfolded protein response (UPR) monitor the unfolded protein load in the ER and convey effector functions for maintaining ER homeostasis. Aberrant compositions of the ER membrane, referred to as lipid bilayer stress, are equally potent activators of the UPR. How the distinct signals from lipid bilayer stress and unfolded proteins are processed by the conserved UPR transducer Ire1 remains unknown. Here, we have generated a functional, cysteine-less variant of Ire1 and performed systematic cysteine crosslinking experiments in native membranes to establish its transmembrane architecture in signaling-active clusters. We show that the transmembrane helices of two neighboring Ire1 molecules adopt an X-shaped configuration independent of the primary cause for ER stress. This suggests that different forms of stress converge in a common, signaling-active transmembrane architecture of Ire1.SummaryThe endoplasmic reticulum (ER) is a hotspot of lipid biosynthesis and crucial for the folding of membrane and secretory proteins. The unfolded protein response (UPR) controls the size and folding capacity of the ER. The conserved UPR transducer Ire1 senses both unfolded proteins and aberrant lipid compositions to mount adaptive responses. Using a biochemical assay to study Ire1 in signaling-active clusters, Väth et al. provide evidence that the neighboring transmembrane helices of clustered Ire1 form an ‘X’ irrespectively of the primary cause of ER stress. Hence, different forms of ER stress converge in a common, signaling-active transmembrane architecture of Ire1.
Endogenous retroviruses are a source of enhancers with oncogenic potential in acute myeloid leukaemia
Authors: Deniz Özgen , Ahmed Mamataz , Todd Christopher D. , Rio-Machin Ana , Dawson Mark A. , Branco Miguel R. 
Published: September 2019

AbstractAcute myeloid leukemia (AML) is a highly aggressive hematopoietic malignancy, defined by a series of genetic and epigenetic alterations, which result in deregulation of transcriptional networks. One understudied but important source of transcriptional regulators are transposable elements (TEs), which are widespread throughout the human genome. Aberrant usage of these sequences could therefore contribute to oncogenic transcriptional circuits. However, the regulatory influence of TEs and their links to disease pathogenesis remain unexplored in AML. Using epigenomic data from AML primary samples and leukemia cell lines, we identified six endogenous retrovirus (ERV) families with AML-associated enhancer chromatin signatures that are enriched in binding of key regulators of hematopoiesis and AML pathogenesis. Using both CRISPR-mediated locus-specific genetic editing and simultaneous epigenetic silencing of multiple ERVs, we demonstrate that ERV deregulation directly alters the expression of adjacent genes in AML. Strikingly, deletion or epigenetic silencing of an ERV-derived enhancer suppressed cell growth by inducing apoptosis in leukemia cell lines. Our work reveals that ERVs are a previously unappreciated source of AML enhancers that have the potential to play key roles in leukemogenesis. We suggest that ERV activation provides an additional layer of gene regulation in AML that may be exploited by cancer cells to help drive tumour heterogeneity and evolution.
An African origin forMycobacterium bovis
Authors: Loiseau Chloé , Menardo Fabrizio , Aseffa Abraham , Hailu Elena , Gumi Balako , Ameni Gobena , Berg Stefan , Rigouts Leen , Robbe-Austerman Suelee , Zinsstag Jakob , Gagneux Sebastien , Brites Daniela 
Published: September 2019

ABSTRACTBackground and objectivesMycobacterium bovisandMycobacterium capraeare two of the most important agents of tuberculosis (TB) in livestock and the most important causes of zoonotic TB in humans. However, little is known about the global population structure, phylogeography and evolutionary history of these pathogens.MethodologyWe compiled a global collection of 3364 whole-genome sequences fromM. bovisandM. capraeoriginating from 35 countries and inferred their phylogenetic relationships, geographic origins and age.ResultsOur results resolved the phylogenetic relationship among the four previously defined clonal complexes ofM. bovis, and another eight newly described here. Our phylogeographic analysis showed thatM. bovislikely originated in East Africa. While some groups remained restricted to East- and West Africa, others have subsequently dispersed to different parts of the world.Conclusions and implicationsOur results allow a better understanding of the global population structure ofM. bovisand its evolutionary history. This knowledge can be used to define better molecular markers for epidemiological investigations ofM. bovisin settings where whole genome sequencing cannot easily be implemented.
Sequence and Structural Determinants of Ligand-dependent Alternating Access of a MATE Transporter
Authors: Jagessar Kevin L. , Claxton Derek P. , Stein Richard A. , Mchaourab Hassane S. 
Published: September 2019

AbstractMATE transporters are ubiquitous ion-coupled antiporters that extrude structurally- and chemically-dissimilar molecules and have been implicated in conferring multidrug resistance. Here, we integrate Double Electron Electron Resonance (DEER) in conjunction with functional assays and site-directed mutagenesis of conserved residues to illuminate principles of ligand-dependent alternating access of PfMATE, a proton-coupled MATE from the hyperthermophilic archaeon Pyrococcus furiosus. Pairs of spin labels monitoring the two sides of the transporter reconstituted into nanodiscs reveal large amplitude movement of helices that alter the orientation of a putative substrate binding cavity. We found that acidic pH favors formation of an inward-facing (IF) conformation, whereas elevated pH (>7) and the substrate rhodamine 6G stabilizes an outward-facing (OF) conformation. PfMATE isomerization between outward-facing and inward-facing conformations is driven by protonation of a previously unidentified intracellular glutamate residue that is critical for drug resistance. Our results can be framed in a mechanistic model of transport that addresses central aspects of ligand coupling and alternating access.
Postcolonial hybridity, diaspora and accountancy
Authors: Kaifala Gabriel Bamie , Gallhofer Sonja , Milner Margaret , Paisey Catriona 
Published: September 2019

Purpose
The purpose of this paper is to explore perceptions and lived experiences of Sierra Leonean chartered and aspiring accountants, vis-à-vis their professional identity with a particular focus on two elements of postcolonial theory, hybridity and diaspora.

Design/methodology/approach
A qualitative methodological framework was employed. Semi-structured interviews were conducted with 18 participants about their perceptions of their professional identity and their professional experiences both within and outside Sierra Leone.

Findings
The current professionalisation process is conceptualised as a postcolonial third space where hybrid professional accountants are constructed. Professional hybridity blurs the local/global praxis being positioned as both local and global accountants. Participants experience difficulty “fitting into” the local accountancy context as a consequence of their hybridisation. As such, a diaspora effect is induced which often culminates in emigration to advanced countries. The paper concludes that although the current model engenders emancipatory social movements for individuals through hybridity and diaspora, it is nonetheless counterproductive for Sierra Leone’s economic development and the local profession in particular.

Research limitations/implications
This study has significant implications for understanding how the intervention of global professional bodies in developing countries shapes the professionalisation process as well as perceptions and lived experiences of chartered and aspiring accountants in these countries.

Originality/value
While extant literature implicates the legacies of colonialism/imperialism on the institutional development of accountancy (represented by recognised professional bodies), this paper employs the critical lens of postcolonial theory to conceptualise the lived experiences of individuals who are directly impacted by such institutional arrangements.
On theoretical engorgement and the myth of fair value accounting in China
Authors: Nobes Christopher 
Published: September 2019

Purpose
The purpose of this paper is to provide “Comments” on two previous papers in this journal about fair value in Chinese accounting. It extends those papers by considering developments since 2006.

Design/methodology/approach
The paper analyses the contents of Chinese Accounting Standards, dividing the references to fair value into several different categories. This analysis is compared to the findings of the two previous papers. This paper then re-assesses the evidence about the alleged pressures from international institutions on Chinese accounting.

Findings
The two previous papers greatly overstate the importance of fair value in Chinese accounting, partly through misinterpreting Chinese standards and partly because of a lack of caveat that the instructions about fair value often relate to special circumstances or unusual companies. The theorising about Chinese enthusiasm for fair value is misguided: the present author suggests that China became keen to adopt international standards despite their use of fair value not because of it, and that China removed much of the fair value when it adapted international standards. The extension of the analysis beyond 2006 provides a fuller coverage but does not alter the conclusions.

Research limitations/implications
The earlier of the two papers examined has been extensively cited. Researchers need to be warned that the technical content and the conclusions of both papers are questionable. Authors should define terms clearly and should provide sufficient reference detail to enable readers to check findings.

Practical implications
Multinational companies, auditors and financial analysts should not be misled into thinking that Chinese accounting makes extensive use of fair value accounting.

Originality/value
This paper critically re-assesses two previous papers, starting with detailed technical data and moving through to the influence of international institutions. This paper also newly extends the analysis of Chinese standards beyond 2006.
The symbol of Saint Gióng and the Gióng festival in the historical context of Vietnam
Authors: Dinh Hai Hong 
Published: September 2019

Purpose
The purpose of this paper is to trace the way in which a popular ritual became one of Vietnam’s most important festivals, elevated as a celebration of national heroism and charts its gradual transformation in modern society.

Design/methodology/approach
This research focuses on the combination of a fertility rite and narratives of St Gióng based on nationalism or heroism created a special festival reflecting many traditional cultural characteristics of Vietnam and the Việt people and the transformation of St Gióng from a mythological to a national symbol of heroism in anti-invader history was recorded in texts.

Findings
The paper casts light on the mythologization and historicization of St Gióng in Vietnam’s particular historical context by decoding the Gióng symbol as a core element of the folktales and myths about St Gióng to understand the formation and development of St Gióng in the cultural history of Vietnam.

Research limitations/implications
The paper is not exploring the Gióng symbol within a larger cultural context of nationalism and ethnosymbolic approach in a comparison of national symbolism and heroism.

Practical implications
The paper includes implications for advised scholars to conduct further exploration of the symbol and myth of not only St Gióng in Vietnam but also Kubera in India and Vaisravana in China to connect Kubera, Vaisravana and St Gióng under the connection of literal myth and heroic symbol.

Social implications
The paper shows how processes of historicizing myth and mythologizing history are important features of Vietnamese socio-historical research.

Originality/value
The paper shows how a fertility rite became a historical festival and the figure of St Gióng became a symbol of patriotic heroism.
Value co-creation for innovation: evidence from Indonesian Organic Community
Authors: Widjojo Handyanto , Fontana Avanti , Gayatri Gita , Soehadi Agus W. 
Published: September 2019

Purpose
The purpose of this paper is to explore how value co-creation in the Indonesian Organic Community overcomes the resource limitations of small enterprises through the integration of collective resources to drive innovation. A framework is derived and developed from service-dominant logic (SDL) and supported by consumer culture theory (CCT). It also offers a specific strategy that is required for the growth and sustainability of the organic-products entrepreneurship.

Design/methodology/approach
Applied thematic analysis was performed by combining observation and in-depth interviews to multi-actors in the community.

Findings
The result shows that a collaboration network with external actors and the dynamic interaction within the community drive resource integration forming value co-creation platform and lead to innovation in product, process, marketing and organization.

Originality/value
A combination of SDL and CCT provides a new marketing perspective of value co-creation concept. SDL offers an understanding of multi-actor value co-creation that is built from the knowledge and skills-based resources. CCT unveils the roles of the community in developing the positive perception of organic products in the market ecosystem.
The determinants of art prices: an analysis of Joan Miró
Authors: Cinefra Jennifer , Garay Urbi , Mibelli Claudia , Pérez Eduardo 
Published: September 2019

Purpose
Relatively little is known about the determinants of the prices of paintings. The purpose of this paper is to analyse the price determinants of the art of Joan Miró, one of the great masters of Modern Art.

Design/methodology/approach
The authors analysed 255 artworks by Miró sold at Sotheby’s and Christie’s between 2003 and 2017, and performed a hedonic price regression to measure the impact of a series of variables on the prices of this artist’s works.

Findings
Miró’s works command higher prices, ceteris paribus, when they were painted on canvas, were sold at Sotheby’s and in New York City or London, were traded during the evening session and depending on the period in which they had been painted, the size of their surface area, the number of words used to describe the respective lot and whether they had appeared in an art book. The prices of Miró’s paintings increased substantially between 2003 and 2008 and then declined, coinciding with the global financial crisis of 2009.

Research limitations/implications
The results were obtained from prices established in art auctions, which represent only one portion of the market.

Originality/value
This is the first exhaustive study carried out on the determinants of the prices of Joan Miró’s works. The artist represents an ideal case due to the large number of his works that have been sold at auctions. As yet, only studies of Pablo Picasso and Andy Warhol have been conducted. Joan Miró has well-defined artistic periods, which also allows us to determine the impact on the price of the works of the period in which it was created. This paper also offers a methodological contribution to parties involved in the art sector (artists, galleries, collectors, investors, museums, etc.).
Aluminum formwork system: loss and productivity
Authors: Abreu Marina Macedo de , Lordsleem Jr Alberto Casado 
Published: September 2019

Purpose
The purpose of this paper is to establish and evaluate the concrete loss and labor productivity (LP) indicators in the concreting step of aluminum formwork system (AFS) in construction in Brazil. The loss and productivity indicators are directed to a regional database (Pernambuco, Brazil).

Design/methodology/approach
Case study was selected as the most appropriate approach. The methodology included data collection in the construction project with 10 residential towers of 320 apartments, in the city of Jaboatão dos Guararapes, Brazil, throughout 82 concrete pouring days using 415 concrete mixer trucks, with a total of 2,582.50 m3 of concrete.

Findings
The findings identified an average concrete loss of 2.6 percent and the LP indicator varying between 0.15 and 0.97 WH/m3. It could be verified that the loss indicators were influenced mainly by the learning effect associated to the qualification of the labor. In addition, the productivity indicators were strongly influenced by delays at the beginning of the concrete pouring and by problems coming from the sequence of concrete supply.

Originality/value
LP indicators are still literature restricted, especially considering only the concreting step. The direct observations of this study allow the identification of factors that inhibit productivity. The comparison of indicators for the concreting service between the ASF and the conventional system attests to the speed, low cost and efficiency of the system studied in this paper.
An exact GITT solution for static bending of clamped parallelogram plate resting on an elastic foundation
Authors: Fu Guangming , Peng Yudan , Sun Baojiang , An Chen , Su Jian 
Published: September 2019

Purpose
The purpose of this study is to propose generalised integral transform technique (GITT) to obtain the exact solutions for bending of clamped parallelogram plate resting on elastic foundation.

Design/methodology/approach
The GITT is used to solve the bending problem of the full clamped parallelogram plate under an elastic foundation. The auxiliary problem was developed and the corresponding eigenfunction and eigenvalue were calculated simultaneously. The original partial differential governed equation has been represented by the transformed ordinary differential equation system and solved by the subroutine DBVPFD from International Mathematics and Statistics Library.

Findings
The GITT has been proven to be an efficient approach to solve the bending problem of the plate with different loads, boundary conditions and elastic foundations. The parametric study indicates that the elastic foundation modulus has significant contribution in reducing the vertical deflections and moments for both rectangular and parallelogram plates. With the increasing of aspect ratio (a/b) and the elastic foundation modulus, the trends of the deflection and moment reduction decreased significantly.

Originality/value
The present hybrid analytical-numerical methodology was first used to solve the mechanics problem of the clamped parallelogram plate resting on elastic foundation. Excellent convergence and high accuracy was observed by comparing with the published results. It exhibits potential application to investigate the mechanics problem of the composite plate with different boundary conditions in the shipbuilding and civil engineering.
The recontextualisation of youth wellbeing in Australian schools
Authors: Whatman Susan , Thompson Roberta , Main Katherine 
Published: September 2019

PurposeThe purpose of this paper is to suggest how well-being messages are recontextualized into school-based contexts from an analysis of national policy and state curricular approaches to health education as reported in the findings of two selected case studies as well as community concerns about young people’s well-being.Design/methodology/approachA cross-sectional review of Australian federal and state-level student well-being policy documents was undertaken. Using two case examples of school-based in-curricular well-being programs, the paper explores how discourses from these well-being policy documents are recontextualized through progressive fields of translation and pedagogic decision making into local forms of curriculum.FindingsPedagogic messages about well-being in Australia are often extra-curricular, in that they are rarely integrated into one or across existing subject areas. Such messages are increasingly focused on mental health, around phenomena such as bullying. Both case examples clearly demonstrate how understandings of well-being respond to various power relations and pressures emanating from stakeholders within and across official pedagogic fields and other contexts such as local communities.Originality/valueThe paper focusses on presenting an adaptation of Bernstein’s (1990) model of social reproduction of pedagogic discourse. The adapted model demonstrates how “top-down” knowledge production from the international disciplines shaping curriculum development and pedagogic approaches can be replaced by community context-driven political pressure and perceived community crises. It offers contemporary insight into youth-at-risk discourses, well-being approaches and student mental health.