bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enhanced pharmacological efficacy of sumatriptan due to modification of its physicochemical properties by inclusion in selected cyclodextrins
Authors: Paczkowska Magdalena , Mizera Mikołaj , Sałat Kinga , Furgała Anna , Popik Piotr , Knapik-Kowalczuk Justyna , Krause Anna , Szymanowska-Powałowska Daria , Fojud Zbigniew , Kozak Maciej , Paluch Marian , Cielecka-Piontek Judyta 
Published: October 2018

AbstractThe study focused on the pharmacological action of sumatriptan, in particular its antiallodynic and antihyperalgesic properties, as an effect of cyclodextrinic inclusion of sumatriptan, resulting in changes of its physicochemical qualities such as dissolution and permeability through artificial biological membranes, which had previously been examinedin vitroin a gastro-intestinal model. The inclusion of sumatriptan into β-cyclodextrin and 2-hydroxylpropylo-β-cyclodextrin by kneading was confirmed with the use of spectral (fourier-transform infrared spectroscopy (FT-IR); solid state nuclear magnetic resonance spectroscopy with magic angle spinning condition,1H and13C MAS NMR) and thermal (differential scanning calorimetry (DSC)) methods. A precise indication of the domains of sumatriptan responsible for its interaction with cyclodextrin cavities was possible due to a theoretical approach to the analysis of experimental spectra. A high-performance liquid chromatography with a diode-array detector method (HPLC-DAD) was employed to determine changes in the concentration of sumatriptan during dissolution and permeability experiments. The inclusion of sumatriptan in complex with cyclodextrins was found to significantly modify its dissolution profiles by increasing the concentration of sumatriptan in complexed form in an acceptor solution compared to in its free form. Following complexation, sumatriptan manifested an enhanced ability to permeate through artificial biological membranes in a gastro-intestinal model for both cyclodextrins at all pH values. As a consequence of the greater permeability of sumatriptan and its increased dissolution from the complexes, an improved pharmacological response was observed when cyclodextrin complexes were applied.
Planarians as models to investigate the bioactivity of gold(I) complexes in vivo
Authors: Tunes Luiza G. , Allen John M. , Zayas Ricardo M. , do Monte-Neto Rubens L. 
Published: October 2018

AbstractGold(I)-containing complexes are used in drug discovery research for rheumatoid arthritis, cancer, and parasitic infections. In this study, we tested the bioactivity of gold(I) complexesin vivousing planarians. The planarianSchmidtea mediterraneapossesses orthologues of tumor suppressor genes, such asp53, that, when silenced, cause deregulation of cell proliferation and apoptosis. In this context, we tested two triethylphosphine-gold(I) complexes (AdO and AdT) to determine if they can attenuate phenotypes that result fromp53inhibition. First, we identified the drug concentration that did not affect survival or regeneration and evaluated the drug’s effect on cell division and apoptosis. We found that AdT treatment decreased the number of mitotic cells and that all drug treatments increased the number of apoptotic cells. We then performedp53(RNAi)and drug treatments concomitantly and observed the phenotype progression. Drug treatment increased survival three-fold and decreased apoptosis, which resulted in an attenuated phenotype. Our results indicate that planarians can be treated with gold(I) complexes, and that this treatment can diminish thep53(RNAi)phenotype and extend survival. In this work we show that planarians can be used as a model to study thein vivoeffect of gold(I) complexes and to further investigate their mechanisms of action.
Microearthquakes preceding a M4.2 Earthquake Offshore Istanbul
Authors: Malin Peter E. , Bohnhoff Marco , Blümle Felix , Dresen Georg , Martínez-Garzón Patricia , Nurlu Murat , Ceken Ulubey , Kadirioglu Filiz Tuba , Kartal Recai Feyiz , Kilic Tugbay , Yanik Kenan 
Published: October 2018

AbstractA primary hurdle in observing small foreshocks is the detection-limit of most seismic networks, which is typically about magnitude M1-1.5. We show that a start-up test of a borehole-based seismic network with a much lower detection limit overcame this problem for an Mw4.2 earthquake. This earthquake occurred offshore of Istanbul, Turkey, on a fault system that is likely to rupture in an M > 7 event in the coming decades. In the three days before and two after, a total of 62 or more earthquakes, including at least 18 foreshocks, came from the mainshock source area. The signal similarity of the foreshocks shows a clear increase during the hours before the Mw4.2 mainshock. Similar foreshock sequences have recently been reported for a few well monitored M > 7 plate-boundary earthquakes. The sequence surrounding the Mw4.2 gives the impression of stochastic failures that ended up interactively unloading stress concentrations. The Mw4.2 mainshock then resulted from the accumulated release of significantly smaller events, as suggested by other field and laboratory studies.
Effect of coffee or coffee components on gut microbiome and short-chain fatty acids in a mouse model of metabolic syndrome
Authors: Nishitsuji Kazuchika , Watanabe Syunsuke , Xiao Jinzhong , Nagatomo Ryosuke , Ogawa Hirohisa , Tsunematsu Takaaki , Umemoto Hitomi , Morimoto Yuki , Akatsu Hiroyasu , Inoue Koichi , Tsuneyama Koichi 
Published: October 2018

AbstractWe previously showed that male Tsumura Suzuki obese diabetes (TSOD) mice, a spontaneous mouse model of metabolic syndrome, manifested gut dysbiosis and subsequent disruption of the type and quantity of plasma short-chain fatty acids (SCFAs), and daily coffee intake prevented nonalcoholic steatohepatitis in this mouse model. Here, we present a preliminary study on whether coffee and its major components, caffeine and chlorogenic acid, would affect the gut dysbiosis and the disrupted plasma SCFA profile of TSOD mice, which could lead to improvement in the liver pathology of these mice. Three mice per group were used. Daily intake of coffee or its components for 16 wk prevented liver lobular inflammation without improving obesity in TSOD mice. Coffee and its components did not repair the altered levels of Gram-positive and Gram-negative bacteria and an increased abundance of Firmicutes in TSOD mice but rather caused additional changes in bacteria in six genera. However, caffeine and chlorogenic acid partially improved the disrupted plasma SCFA profile in TSOD mice, although coffee had no effects. Whether these alterations in the gut microbiome and the plasma SCFA profile might affect the liver pathology of TSOD mice may deserve further investigation.
Uranium (VI) detection in groundwater using a gold nanoparticle/paper-based lateral flow device
Authors: Quesada-González Daniel , Jairo Grace A. , Blake Robert C. , Blake Diane A. , Merkoçi Arben 
Published: October 2018

AbstractThe contamination in groundwater due to the presence of uranium is nowadays a subject of concern due to the severe health problems associated with renal failure, genotoxicity and cancer. The standard methods to detect uranium require time-consuming processes and expensive non-portable equipment, so these measurements are rarely performed in-field, which increases the time until water samples are analysed. Furthermore, the few portable methods available do not allow quantitative analysis and the detection limit is often not low enough to reach the recommendations for drinking water (30 ppb or 126 nM of uranium). For the first time, we propose a portable, fast, inexpensive and sensitive paper-based biosensor able to detect in situ U(VI) in water samples: U(VI) selective gold nanoparticle-based lateral flow strips. Antibody-coated gold nanoparticles are used as labels in the proposed lateral flow system because of their biocompatibility; in addition, these nanoparticles provide high sensitivity due to their intense plasmonic effect. The antibody used in the assay recognizes soluble U(VI) complexed to the chelator, 2,9-dicarboxyl-1,10-phenanthroline (DCP). Because of the small size of the U(VI)-DCP complex, this assay employs a competitive format that reaches a limit of detection of 36.38 nM, lower than the action level (126 nM) established by the World Health Organization and the U.S. Environmental Protection Agency for drinking waters.
Genome-wide Identification of Jatropha curcas MAPK, MAPKK, and MAPKKK Gene Families and Their Expression Profile Under Cold Stress
Authors: Wang Haibo , Gong Ming , Guo Junyun , Xin Hu , Gao Yong , Liu Chao , Dai Dongqin , Tang Lizhou 
Published: October 2018

AbstractMitogen-activated protein kinase (MAPK) cascades are fundamental signal transduction modules in all eukaryotic organisms, controlling cell division, growth, development, and hormone signaling. Additionally, they can be activated in response to a variety of biotic and abiotic stressors. Although the evolution and expression patterns of MAPK cascade families have been systematically investigated in several model plants (e.g.,Arabidopsis, rice, and poplar), we still know very little aboutMAPK,MAPKK, andMAPKKKfamilies inJatropha curcas, an economically important species. Therefore, this study performed genome-wide identification and transcriptional expression analysis of these three families inJ. curcas. We identified 12 J. curcas MAPK(JcMAPKs), 5JcMAPKKs, and 65JcMAPKKKs. Phylogenetic analysis classified allJcMAPKsandJcMAPKKsinto four subgroups, whereasJcMAPKKKswere grouped into three subfamilies (MEKK, RAF, and ZIK). Similarities in exon/intron structures supported the evolutionary relationships within subgroups and subfamilies. Conserved motif analysis indicated that allJ. curcasMAPK cascades possessed typical, 200–300 amino-acid protein kinase domains. MAPK cascade genes were presented throughout all 11 chromosomes. Gene duplication analysis suggested that afterJcMAPKandJcMAPKKKdiverged, 3 and 19 tandem duplicates occurred under strong purifying selection. Furthermore, RNA-seq and qRT-PCR analyses revealed that some MAPK cascade genes are predominantly expressed in specific tissues. Moreover, their expression levels significantly increased under cold treatment. Our results should provide insight into the roles of MAPK cascade genes in regulatingJ. curcasstress responses and in hormonal signal transduction. Furthermore, these data have important applications in the genetic improvement ofJ. curcas.
Randomised Clinical Trial for Postoperative Complications after Ex-PRESS Implantation versus Trabeculectomy with 2-Year Follow-Up
Authors: Arimura Shogo , Miyake Seiji , Iwasaki Kentaro , Gozawa Makoto , Matsumura Takehiro , Takamura Yoshihiro , Inatani Masaru 
Published: October 2018

AbstractWe compared complications between Ex-PRESS implantation and trabeculectomy for 2 years after surgery. Sixty-four open-angle glaucoma eyes were randomly assigned to treatment with trabeculectomy (n = 32) or Ex-PRESS implantation (n = 32). The primary outcomes were postoperative complications, including reduction of the endothelial cell density (ECD) of the cornea, cataract progression and the frequency of other late postoperative complications. The Ex-PRESS group had significantly greater reduction of postoperative corneal ECD than the trabeculectomy group did at 2 years after surgery (P = 0.026). Among the corneal areas measured using specular microscopy, the superior area, where the Ex-PRESS tube was inserted, had significantly more severe corneal ECD reduction than the inferior area after 2 years (−17.6% in superior area and −11.7% in inferior area, P = 0.04). More cataract progression occurred in the trabeculectomy group than in the Ex-PRESS group (P = 0.04). Twelve eyes (37.5%) in the trabeculectomy group and 4 eyes (12.5%) in the Ex-PRESS group underwent cataract surgery (P = 0.019). The total number of other postoperative complications between 3 months and 2 years was significantly higher in the trabeculectomy group than in the Ex-PRESS group (P = 0.02). Although Ex-PRESS implantation might be associated with an increased rate of corneal endothelial cell loss compared with trabeculectomy, it is beneficial for preventing cataract progression after filtering surgery.
Impact of Cardiovascular Magnetic Resonance Imaging on Identifying the Etiology of Cardiomyopathy in Patients Undergoing Cardiac Transplantation
Authors: Lin Lucy Q. , Kazmirczak Felipe , Chen Ko-Hsuan Amy , Okasha Osama , Nijjar Prabhjot S. , Martin Cindy M. , Akçakaya Mehmet , Farzaneh-Far Afshin , Shenoy Chetan 
Published: October 2018

AbstractErrors in identifying the etiology of cardiomyopathy have been described in patients undergoing cardiac transplantation. There are increasing data that cardiovascular magnetic resonance imaging (CMR) provides unique diagnostic information in heart failure. We investigated the association of the performance of CMR prior to cardiac transplantation with rates of errors in identifying the etiology of cardiomyopathy. We compared pre-transplantation clinical diagnoses with post-transplantation pathology diagnoses obtained from the explanted native hearts. Among 338 patients, there were 23 (7%) errors in identifying the etiology of cardiomyopathy. Of these, 22 (96%) occurred in patients with pre-transplantation clinical diagnoses of non-ischemic cardiomyopathy (NICM). Only 61/338 (18%) had CMRs prior to transplantation. There was no significant association between the performance of CMR and errors in the entire study cohort (p = 0.093). Among patients with pre-transplantation clinical diagnoses of NICM, there was a significant inverse association between the performance of CMR and errors (2.4% vs. 14.6% in patients with and without CMR respectively; p = 0.030). In conclusion, CMR was underutilized prior to cardiac transplantation. In patients with pre-transplantation clinical diagnoses of NICM – in whom 96% of errors in identifying the etiology of cardiomyopathy occurred – the performance of CMR was associated with significantly fewer errors.
Molecular signature of the putative stem/progenitor cells committed to the development of the bovine mammary gland at puberty
Authors: Finot Laurence , Chanat Eric , Dessauge Frederic 
Published: October 2018

AbstractMilk production is highly dependent on the extensive development of the mammary epithelium, which occurs during puberty. It is therefore essential to distinguish the epithelial cells committed to development from the related epithelial hierarchy. Using cell phenotyping and sorting, we highlighted four cell sub-populations within the bovine mammary gland at puberty. The CD49fhighCD24neg cells expressing CD10, KRT14, vimentin and PROCR corresponded to cells committed to the basal lineage. The CD49flow sub-population contained two cell subsets (CD49flowCD24neg and CD49flowCD24pos). Both subsets expressed hormone receptors including ER, PR and PRLR, as well as ALDH1 activity but only the CD49flowCD24pos subset expressed ELF5. These data indicated that the CD49flow sub-population is mainly composed of cells displaying a luminal phenotype and that this population comprises two luminal cell subsets, namely the CD24neg and CD24pos cells, likely committed to ductal and alveolar lineage, respectively. The putative mammary stem cell (MaSC) fraction was recovered in the CD49fhighCD24pos sub-population which were shown to form mammospheres in vitro. These cells differentially expressed CD10, KRT14 and KRT7, suggesting the existence of several putative MaSC sub-fractions. In-depth characterization of these epithelial sub-populations provides new insights into the bovine mammary epithelial cell lineage and suggests a common developmental lineage in mammals.
Early Identification of Dyslexia: Understanding the Issues
Authors: Colenbrander Danielle , Ricketts Jessie , Breadmore Helen L. 
Published: October 2018

Purpose
The purpose of this tutorial is to provide an overview of the benefits and challenges associated with the early identification of dyslexia.

Method
The literature on the early identification of dyslexia is reviewed. Theoretical arguments and research evidence are summarized. An overview of response to intervention as a method of early identification is provided, and the benefits and challenges associated with it are discussed. Finally, the role of speech-language pathologists in the early identification process is addressed.

Conclusions
Early identification of dyslexia is crucial to ensure that children are able to maximize their educational potential, and speech-language pathologists are well placed to play a role in this process. However, early identification alone is not sufficient—difficulties with reading may persist or become apparent later in schooling. Therefore, continuing progress monitoring and access to suitable intervention programs are essential.
What Speech-Language Pathologists Need to Know About Dyslexia
Authors: Hogan Tiffany P. 
Published: October 2018

Purpose
The purpose of this clinical forum is to provide a broad survey of up-to-date, evidence-based information about dyslexia that can be readily applied to clinical practice by speech-language pathologists. Topics include (a) theoretical and clinical frameworks for understanding dyslexia in relation to other language-based communication disorders, (b) the neurobiological basis of dyslexia, (c) the current state of dyslexia legislation, (d) issues around identification of dyslexia, (e) and evidence-based reviews of instruction for reading and writing.

Conclusion
Though there have been numerous papers written on the topic of dyslexia, this clinical forum is unique in that it is the only combination of articles to focus on dyslexia with the speech-language pathologist in mind.
High step‐up DC–DC converter with coupled inductor suitable for renewable applications
Authors: Pourjafar Saeed , Sedaghati Farzad , Shayeghi Hossein , Maalandish Mohammad 
Published: October 2018

In this study, a new non‐isolated high step‐up DC–DC converter is presented with high‐voltage gain which is suitable for renewable applications. The proposed converter uses coupled inductor and voltage multiplier cell (diode capacitor) for increasing the voltage level. The voltage gain of the proposed converter can be increased by selecting the appropriate turns ratio of coupled inductor. Voltage multiplier cell consists of two diodes and two capacitors which are used to obtain high‐voltage gain. The diode‐capacitor cell is used as a clamp circuit, which leads to reducing the voltage stress across the semiconductors. The proposed converter has a single power switch which causes the control of the proposed converter is simple. Also, the power switch is used with lower ON‐state resistant (RDS‐ON). The zero‐current switching of the diode is obtained in OFF state. Therefore, the conduction losses are decreased with lower normalised voltage stress across semiconductors. To prove the performance of the proposed converter, theoretical analysis and comparison with other converters are provided. To confirm the benefits of the proposed converter, a laboratory prototype with 20 V input voltage, 200 V output voltage and about 200 W power level at operating 25 kHz is built and tested.
Dynamic interaction between synchronous machine and DC‐power‐modulated LCC in electromechanical timescale
Authors: Hou Jiazuo , Ma Shicong , Gong Xuan , Hu Jiabing 
Published: October 2018

Active power modulation of line‐commutated converter (LCC)‐based high‐voltage direct current (HVDC) system is increasingly utilised, especially for the case of damping low‐frequency oscillation associated with synchronous machine (SM) in electromechanical timescale (around 1 Hz). Many papers have tried to analyse the mechanism of damping effects, but almost rely on numerical studies, which cannot reveal the dynamic interaction between SM and DC‐power‐modulated LCC. This study proposes a small‐signal model of DC‐power‐modulated LCC based on motion equation concept. With this model, analytical investigation of low‐frequency oscillation from the scope of damping and synchronising powers is presented. Comparisons of analytical results and eigenvalues draw some general conclusions which offer insight into the dynamic behaviour. By examining the case of an SM connected to a DC‐power‐modulated LCC, simulations in MATLAB/Simulink are conducted to verify the analytical results.
Research of non‐destructive testing for aluminium sheaths of HV cables using ultrasonic‐guided waves
Authors: Junping Cao , Hang Jiang , Shaohua Wang , Yong Yang , Luyao Zhou , Peng Jiang 
Published: October 2018

The method of fast non‐destructive testing for aluminium sheaths of high‐voltage (HV) cables using ultrasonic‐guided waves is proposed. The dispersion curve is calculated by solving the dispersion equation from the simplified model of aluminium sheaths of HV cables; thus, the appropriate wave mode and excitation frequency can be chosen. Three defects including crack, perforation, and sag have been set up. Then, the simulation experiments have been carried out for aluminium sheaths of HV cables based on the theoretical analysis and finite element simulation. The results show that cracks with the loss ratio of the sectional area over 4.25% can be detected by the T (0, 1) mode whose frequency is 32 kHz; the amplitude of the flaw echo increases along with the loss ratio of the crack. Theoretical analysis, finite element simulation, and experiments prove the feasibility of aluminium sheaths using ultrasonic‐guided waves, providing a new method for the defect testing.