bluuebunny/crossref_metadata_2025_split huggingface dataset:
An approach for vascular tumor growth based on a hybrid embedded/homogenized treatment of the vasculature within a multiphase porous medium model
Authors: Kremheller Johannes , Vuong Anh‐Tu , Schrefler Bernhard A. , Wall Wolfgang A. 
Published: August 2019

AbstractThe aim of this work is to develop a novel computational approach to facilitate the modeling of angiogenesis during tumor growth. The preexisting vasculature is modeled as a 1D inclusion and embedded into the 3D tissue through a suitable coupling method, which allows for nonmatching meshes in 1D and 3D domain. The neovasculature, which is formed during angiogenesis, is represented in a homogenized way as a phase in our multiphase porous medium system. This splitting of models is motivated by the highly complex morphology, physiology, and flow patterns in the neovasculature, which are challenging and computationally expensive to resolve with a discrete, 1D angiogenesis and blood flow model. Moreover, it is questionable if a discrete representation generates any useful additional insight. By contrast, our model may be classified as a hybrid vascular multiphase tumor growth model in the sense that a discrete, 1D representation of the preexisting vasculature is coupled with a continuum model describing angiogenesis. It is based on an originally avascular model which has been derived via the thermodynamically constrained averaging theory. The new model enables us to study mass transport from the preexisting vasculature into the neovasculature and tumor tissue. We show by means of several illustrative examples that it is indeed capable of reproducing important aspects of vascular tumor growth phenomenologically.
Activity‐Based Kinome Profiling Using Chemical Proteomics and ATP Acyl Phosphates
Authors: Franks Caroline E. , Hsu Ku‐Lung 
Published: August 2019

AbstractHuman kinases are a large family of proteins (500+) that catalyze ATP‐dependent phosphorylation of protein and metabolite substrates to regulate diverse facets of cell biology. Dysregulation and mutations of protein kinases are linked to human disease, providing opportunities for developing pharmacological agents as potential therapy. Assessing the selectivity of pharmacological compounds targeting this enzyme class is critical given that off‐target activity of kinase inhibitor drugs may result in toxicity. This set of protocols outlines use of ATP acyl phosphate activity–based probes to evaluate the potency and selectivity of kinase inhibitors via fluorescent gel– and mass spectrometry–based detection methods. These competitive chemical proteomic assays can evaluate engagement of >200 native kinase targets directly in complex proteomes. © 2019 by John Wiley & Sons, Inc.
Site‐ and Structure‐Specific Quantitative N‐Glycoproteomics Using RPLC‐pentaHILIC Separation and the Intact N‐Glycopeptide Search Engine GPSeeker
Authors: Xiao Kaijie , Tian Zhixin 
Published: August 2019

AbstractSite‐ and structure‐specific quantitative N‐glycoproteomics characterization of differentially expressed N‐glycosylation at the intact N‐glycopeptide level with distinct chromatographic separation and structure‐specific fragment ions has become possible with the recent development of RPLC‐pentaHILIC 2DLC separation and use of the intact N‐glycopeptide search engine GPSeeker. Here we provide a detailed protocol for this GPSeeker‐centered structure‐specific isotopic‐labeling quantitative N‐glycoproteomics pipeline. The protocols include sample preparation of a 1:1 mixture of light (‐CH3)2 and heavy (‐13CD2H)2 dimethylated intact N‐glycopeptides from LO2 and HepG2 cells, RPLC‐pentaHILIC 2DLC separation of the mixture, intact N‐glycopeptide database search and identification using GPSeeker, and quantitation of differentially expressed intact N‐glycopeptides using the quantitation module GPSeekerQuan. © 2019 by John Wiley & Sons, Inc.
Detection of Δ9 THC in oral fluid following vaporized cannabis with varied cannabidiol (CBD) content: An evaluation of two point‐of‐collection testing devices
Authors: Arkell Thomas R. , Kevin Richard C. , Stuart Jordyn , Lintzeris Nicholas , Haber Paul S. , Ramaekers Johannes G. , McGregor Iain S. 
Published: August 2019

AbstractPoint‐of‐collection testing (POCT) for Δ9‐tetrahydrocannabinol (THC) in oral fluid is increasingly used to detect driving under the influence of cannabis (DUIC). However, previous studies have questioned the reliability and accuracy of two commonly used POCT devices, the Securetec DrugWipe® 5 s (DW5s) and Dräger DrugTest® 5000 (DT5000). In the current placebo controlled, double‐blind, crossover study we used liquid chromatography‐tandem mass spectrometry (LC–MS/MS) to accurately quantify cannabinoid concentrations in the oral fluid of 14 participants at various timepoints (10, 60, 120, and 180 minutes) following vaporization of 125 mg of THC‐dominant (11% THC; <1% CBD), THC/CBD equivalent (11% THC; 11% CBD) and placebo (<1% THC; <1% CBD) cannabis. At each timepoint, oral fluid was also screened using the DW5s (10 ng/mL THC cut‐off) and DT5000 (10 ng/mL THC cut‐off). LC–MS/MS analysis showed peak oral fluid THC concentrations at the 10 minute timepoint with a rapid decline thereafter. This trajectory did not differ with THC dominant and THC/CBD equivalent cannabis. With a 10 ng/mL confirmatory cut‐off, 5% of DW5s test results were false positives and 16% false negatives. For the DT5000, 10% of test results were false positives and 9% false negatives. Neither the DW5s nor the DT5000 demonstrated the recommended >80% sensitivity, specificity and accuracy. Accuracy was lowest at 60 minutes, when THC concentrations were often close to the screening cut‐off (10 ng/mL). POCT devices can be useful tools in detecting recent cannabis use; however, limitations should be noted, and confirmatory LC–MS/MS quantification of results is strongly advisable.
Religiosity and the value of being moral: Understanding the meaning of morality through a personal values perspective
Authors: Sverdlik Noga , Rechter Eyal 
Published: August 2019

AbstractWe demonstrate how religiosity predicts relationships between personal values and perceptions of morality in four studies across two cultures (Jews in Israel and mainly Christians in the US). In Studies 1A (N = 337) and 1B (N = 200), we explored the commonalities and differences between religious and non‐religious participants in the association between values and the importance of being moral. In Studies 2A (N = 131) and 2B (N = 250), we tested the role of religiosity in the association between values and evaluations of others’ morality. Power (negatively) and Benevolence (positively) were associated with morality across levels of religiosity. The associations with Conservation were more positive for more religious participants; the associations with Universalism were more positive for less religious participants. Finally, the associations with at least one of the Openness‐to‐change values were more negative if a person was more religious. Studies 2A and 2B showed these associations existed over and above differences in personal values.
Determination of Imidacloprid Based on the Development of a Glassy Carbon Electrode Modified with Reduced Graphene Oxide and Manganese (II) Phthalocyanine
Authors: Paula Santos Anna , Ferreira Oliveira Ana Elisa , César Pereira Arnaldo 
Published: August 2019

AbstractAn electrochemical sensor of glassy carbon electrode modified with reduced graphene oxide and manganese (II) phthalocyanine (GCE/rGO/MnPc) was developed as an effective alternative in the determination of imidacloprid in honey samples. The peak current variation obtained with the proposed sensor, in the presence of imidacloprid, was higher compared to the bare GCE. The followed experimental conditions were optimized: reduced graphene oxide concentration (2.0 mg mL−1), manganese (II) phthalocyanine concentration (1.5 mg mL−1), electrolyte pH (6.5) and electrolyte concentration (1,50 mol L−1). The study also showed that the process of reduction of imidacloprid is irreversible and diffusion‐controlled, with a single reduction peak of approximately −0.9 V corresponding to the reduction of the nitro group (−NO2) present in the structure, generating a derived from hydroxylamine, in a process involving about four electrons. The determination of imidacloprid in honey samples exhibited recovery values within the EPA range (between 90.5 and 101.9 %). The proposed sensor GCE/rGO/MnPc can be used as an effective alternative in the determination of imidacloprid in honey samples.
Slippage mutation rates in 15 autosomal short tandem repeat loci for forensic purposes in a Southeastern Brazilian population
Authors: Hamester Fernanda Irma Remus , da Silva Débora Santos , Leboute Ana Paula Magalhães , Motta Carlos Henrique Ares , Alho Clarice Sampaio 
Published: August 2019

AbstractWell‐defined estimates of mutation rates in highly polymorphic tetranucleotide STR loci are a prerequisite for human identification in genetics laboratory routines useful for civil and criminal investigations. Studying 15 autosomal STR loci of forensic interest (CSF1PO, D2S1338, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D19S433, D21S11, FGA, TH01, TPOX, and vWA), we detected 193 slippage mutations (189 one‐step and four two‐step mutations) in 148 875 parent‐child allelic transfers from 5171 paternity cases with true biological relationship (15 096 individuals; 4754 trios and 417 duos; 9925 meiosis) from the state of São Paulo, a very representative population of Brazil. The overall mutation rate was 1.3 × 10−3 and the highest rates were observed at loci vWA (2.8 × 10−3), FGA and D18S51 (2.7 × 10−3 for both), while loci TH01 and TPOX did not present any mutations. The mean slippage mutation rate of paternal origin (1.8 × 10−3) was six times higher than that observed for maternal origin (0.3 × 10−3).
Acetate formation during recombinant protein production in Escherichia coli K‐12 with an elevated NAD(H) pool
Authors: Han Qi , Eiteman Mark A. 
Published: August 2019

AbstractAcetate formation is a disadvantage in the use of Escherichia coli for recombinant protein production, and many studies have focused on optimizing fermentation processes or altering metabolism to eliminate acetate accumulation. In this study, E. coli MEC697 (MG1655 nadR nudC mazG) maintained a larger pool of NAD(H) compared to the wild‐type control, and also accumulated lower concentrations of acetate when grown in batch culture on glucose. In steady‐state cultures, the elevated total NAD(H) found in MEC697 delayed the threshold dilution rate for acetate formation to a growth rate of 0.27 h−1. Batch and fed‐batch processes using MEC697 were examined for the production of β‐galactosidase as a model recombinant protein. Fed‐batch culture of MEC697/pTrc99A‐lacZ compared to MG1655/pTrc99A‐lacZ at a growth rate of 0.22 h−1 showed only a modest increase of protein formation. However, 1 L batch growth of MEC697/pTrc99A‐lacZ resulted in 50% lower acetate formation compared to MG1655/pTrc99A‐lacZ and a two‐fold increase in recombinant protein production.
Seismic performance and design of bridge column‐to‐pile shaft pipe‐pin connections in precast and cast‐in‐place bridges
Authors: Mehraein Mehrdad , Saiidi Saiid 
Published: August 2019

SummaryHinge or “pin” connections may be used in integral bridges to connect columns to pile shafts to reduce the foundation force demand. Used in combination with prefabricated columns, pins facilitate accelerated bridge construction (ABC). These innovative methods could improve the quality and economy of project compared with conventional construction in seismic regions. This study developed pipe pins that reduce moment transfer between the column and pile shaft under seismic excitations. The pipe pins consist of two steel pipes and a rod that transfer shear and tension while allowing rotation between the column and shaft. The primary objective of this research was to investigate the seismic performance and develop design guidelines of column‐to‐pile shaft pipe pins for cast‐in‐place and precast constructions. This research was composed of experimental and analytical studies. The experimental portion of the study consisted of testing of a large‐scale bent model subjected to seismic loadings. The test results confirmed that the proposed design method meets the safety and performance requirements of the codes under seismic loadings. The pins maintained structural integrity with minimal damage, while the columns reached the full plastic hinge capacity. The analytical studies consisted of (a) a simple stick model to be used as a design tool, (b) a finite element model (FEM) for global analysis of bridges, and (c) an elaborate FEM to investigate the microscopic performance and interaction of the components. The analytical models were subsequently used in parametric studies.
Mechanical behavior of lead rubber bearings under and after nonproportional plane loading
Authors: Wang Shiang‐Jung , Lin Wang‐Chuen , Chiang Yu‐Sheng , Hwang Jenn‐Shin 
Published: August 2019

SummaryLead rubber bearings, which have been extensively applied in many seismic isolation designs for buildings, infrastructures, and facilities worldwide, were tested under unilateral reversal loading as well as nonproportional plane loading including circular, figure‐eight, and square orbits in this study. The test results indicate that unlike the unilateral hysteretic behavior, the bilateral one of lead rubber bearings is too complicated to be characterized adequately by a simplified bilinear hysteretic model. It is mainly attributed to the bilateral coupling effect, which can be clearly observed from the abnormal deformation of the mesh pattern drawn on the rubber cover during the tests. In addition, after being subjected to nonproportional plane loading, the tested bearings reveal visible permanent twisting deformation. The profiles of the cut bearings present the fracture of the inside lead plugs. Even so, the further unilateral reversal loading test results prove that the fracture might not affect the whole hysteretic behavior and mechanical properties very much. The applicability, robustness, and generalization of adopting three previously developed analytical models for describing the coupled bilateral hysteretic behavior of lead rubber bearings are further demonstrated by comparing their predictions with the nonproportional plane loading test results. Although the coefficients are identified from unilateral reversal loading tests, the three analytical models can still have an acceptable prediction capability.
Relationship Between Methylmercury Contamination and Proportion of Aquatic and Terrestrial Prey in Diets of Shoreline Spiders
Authors: Ortega-Rodriguez Celeste L. , Chumchal Matthew M. , Drenner Ray W. , Kennedy James H. , Nowlin Weston H. , Barst Benjamin D. , Polk D. Kirkland , Hall MacGregor N. , Williams Edward B. , Lauck Kyle C. , Santa-Rios Andrea , Basu Niladri 
Published: August 2019

Abstract
Terrestrial organisms such as shoreline spiders that consume prey from aquatic food webs can be contaminated with methylmercury (MeHg). However, no studies have examined the relationship between MeHg contamination of shoreline spider taxa and the proportion of aquatic and terrestrial prey in their diets. The present study had 2 objectives: 1) determine concentrations of MeHg in 7 taxa of shoreline spiders, and 2) assess the relationship between concentrations of MeHg in spiders and the proportion of aquatic and terrestrial prey in spider diets. We collected shoreline spiders, emergent aquatic insects, and terrestrial insects from in and around 10 experimental ponds. Methylmercury concentrations were greatest in spiders, intermediate in aquatic insects, and lowest in terrestrial insects. The elevated MeHg concentrations in spiders indicate that they were feeding, at least in part, on emergent aquatic insects. However, variability in MeHg concentration observed among spider taxa suggested that the proportion of aquatic and terrestrial prey in spider diets likely varied among taxa. We estimated the proportion of aquatic and terrestrial prey in the diet of each spider taxon from the nitrogen (δ15N) and carbon (δ13C) isotope values of spiders and their potential aquatic and terrestrial prey items. The median proportion of aquatic prey in spider diets varied by almost 2-fold, and MeHg concentrations in shoreline spiders were strongly correlated with the proportion of aquatic prey in their diet. In the present study, we demonstrate for the first time that the degree of connectivity to aquatic food webs determines MeHg contamination of shoreline spiders. Environ Toxicol Chem 2019;38:2503–2508. © 2019 SETAC
Abstract
The concentration of MeHg in spider taxa increased as a function of the proportion of aquatic prey in their diet, demonstrating for the first time that the degree of connectivity to aquatic food webs determines MeHg contamination of shoreline spiders.
X‐ray computed tomography for quality inspection of agricultural products: A review
Authors: Du Zhe , Hu Yongguang , Ali Buttar Noman , Mahmood Ashraf 
Published: August 2019

AbstractThe quality of agricultural products relates to the internal structure, which has long been a matter of interest in agricultural scientists. However, inspection methods of the opaque nature of internal information on agricultural products are usually destructive and require sample separation or preparation. X‐ray computed tomography (X‐ray CT) technology is one of the important nondestructive testing (NDT) technologies without sample separation and preparation. In this study, X‐ray CT technology is used to obtain two‐dimensional slice images and three‐dimensional tomographic images of samples. The purpose of the review was to provide an overview of the working principle of X‐ray CT technology, image processing, and analysis. This review aims to focus on the development of the agricultural products (e.g., wheat, maize, rice, apple, beef) and its applications (e.g., internal quality evaluation, microstructure observation, mechanical property measurement, and others) using CT scanner. This paper covers the aspects regarding the advantages and disadvantages of NDT technology, especially the unique advantages and limitations of X‐ray CT technology on the quality inspection of agricultural products. Future prospects of X‐ray CT technology are also put forward to become indispensable to the quality evaluation and product development on agricultural products.
The Ensemble Mars Atmosphere Reanalysis System (EMARS) Version 1.0
Authors: Greybush Steven J. , Kalnay Eugenia , Wilson R. John , Hoffman Ross N. , Nehrkorn Thomas , Leidner Mark , Eluszkiewicz Janusz , Gillespie Hartzel E. , Wespetal Matthew , Zhao Yongjing , Hoffman Matthew , Dudas Patrick , McConnochie Timothy , Kleinböhl Armin , Kass David , McCleese Daniel , Miyoshi Takemasa 
Published: August 2019

AbstractThe Ensemble Mars Atmosphere Reanalysis System (EMARS) dataset version 1.0 contains hourly gridded atmospheric variables for the planet Mars, spanning Mars Year (MY) 24 through 33 (1999 through 2017). A reanalysis represents the best estimate of the state of the atmosphere by combining observations that are sparse in space and time with a dynamical model and weighting them by their uncertainties. EMARS uses the Local Ensemble Transform Kalman Filter (LETKF) for data assimilation with the GFDL/NASA Mars Global Climate Model (MGCM). Observations that are assimilated include the Thermal Emission Spectrometer (TES) and Mars Climate Sounder (MCS) temperature retrievals. The dataset includes gridded fields of temperature, wind, surface pressure, as well as dust, water ice, CO2 surface ice and other atmospheric quantities. Reanalyses are useful for both science and engineering studies, including investigations of transient eddies, the polar vortex, thermal tides and dust storms, and during spacecraft operations.
Inhibitory milieu at the multiple sclerosis lesion site and the challenges for remyelination
Authors: Galloway Dylan A. , Gowing Elizabeth , Setayeshgar Solmaz , Kothary Rashmi 
Published: August 2019

AbstractRegeneration of myelin, following injury, can occur within the central nervous system to reinstate proper axonal conductance and provide trophic support. Failure to do so renders the axons vulnerable, leading to eventual degeneration, and neuronal loss. Thus, it is essential to understand the mechanisms by which remyelination or failure to remyelinate occur, particularly in the context of demyelinating and neurodegenerative disorders. In multiple sclerosis, oligodendrocyte progenitor cells (OPCs) migrate to lesion sites to repair myelin. However, during disease progression, the ability of OPCs to participate in remyelination diminishes coincident with worsening of the symptoms. Remyelination is affected by a broad range of cues from intrinsic programming of OPCs and extrinsic local factors to the immune system and other systemic elements including diet and exercise. Here we review the literature on these diverse inhibitory factors and the challenges they pose to remyelination. Results spanning several disciplines from fundamental preclinical studies to knowledge gained in the clinic will be discussed.
HPV‐negative and HPV‐positive HNSCC cell lines show similar numerical but different structural chromosomal aberrations
Authors: Arenz Andrea , Patze Johannes , Kornmann Evelyn , Wilhelm Jochen , Ziemann Frank , Wagner Steffen , Wittig Andrea , Schoetz Ulrike , Engenhart‐Cabillic Rita , Dikomey Ekkehard , Fritz Barbara 
Published: August 2019

AbstractBackgroundIt was tested whether the difference in carcinogenesis between noxa and human papillomavirus (HPV)‐driven head and neck squamous cell carcinoma (HNSCC) is associated with a variation in genomic instability.MethodsConventional and molecular cytogenetics in HPV‐positive and HPV‐negative HNSCC cell lines.ResultsNumerical aneuploidy determined by multicolor fluorescence in situ hybridization and DNA ploidy was very similar for both entities with most chromosomes being present either in quadruplicate or triplicate, and only few are still diploid with, however, a striking similarity in the overall pattern. A clear difference was seen concerning the translocations formed, with no difference in the total amount but with a significantly higher genomic instability of HPV‐positive cell lines at chromosome 3 as compared to HPV‐negative cells.ConclusionThe different processes of carcinogenesis of HPV‐positive and HPV‐negative HNSCC appear to result in a similar pattern of numerical but a clear difference in structural chromosomal aberrations.
Human resource management systems, employee well‐being, and firm performance from the mutual gains and critical perspectives: The well‐being paradox
Authors: Ho Hoang , Kuvaas Bård 
Published: August 2019

AbstractIn this study, we explored the additive, interactive, and nonlinear relationships among human resource management (HRM) systems, employee well‐being, and firm performance. Based on a sample of 14,384 employees nested within 1,347 firms, we obtained three main findings. First, HRM systems yield a performance effect that exceeds the effect of single practice, suggesting positive synergies among HRM practices. Second, the opportunity bundle has a positive impact on firm performance, but when integrating it with skills and motivation bundles, the result becomes negative, indicating dis‐synergy of interactions among HRM bundles. Third, at moderate levels of adoption, HRM practices are positively correlated with employee well‐being and higher levels of commitment, job satisfaction, and management relations, as well as lower levels of anxiety. However, at high levels, the relationship is less positive and even turns negative with lower levels of job satisfaction and management relations. To close, we present research implications and future directions after discussing our results.