bluuebunny/crossref_metadata_2025_split huggingface dataset:
Omeprazole‐induced hypertrichosis in two children
Authors: Elosua‐González Marta , Campos‐Domínguez Minia , Bancalari Daniel , Noguera‐Morel Lucero , Hernández‐Martín Angela , Huerta‐Aragonés Jorge , Torrelo Antonio 
Published: March 2018

AbstractOmeprazole significantly increases duodenal prostaglandin E2 synthesis. Prostaglandins are involved in hair growth regulation: prostaglandin E2 and prostaglandin F2 alpha stimulate hair growth, and prostaglandin D2 has an inhibitory effect. The use of omeprazole can cause acquired generalized hypertrichosis by increasing prostaglandin E2 levels.
Efficacy and tolerability of magnesium plus protein for managing hypomagnesemia in pediatric kidney transplant patients
Authors: Lewis Teresa V. , Neely Stephen , Turman Martin A. 
Published: March 2018

AbstractWe sought to investigate whether magnesium oxide bound to soy protein (MGP) increases serum magnesium concentrations with less diarrhea compared to commonly prescribed magnesium salts. Subjects were switched to MGP at a near‐equivalent daily elemental magnesium dose. Mean serum magnesium levels were compared. If magnesium levels remained <1.7 mg/dL after switching to MGP, subjects were enrolled into Part 2 and received a one‐time MGP dose adjustment. The MGP daily dose was increased by 266 mg. For both parts 1 and 2, subjects recorded the number and quality of their stools to assess gastrointestinal (GI) tolerability of MGP. Twelve pediatric kidney transplant recipients completed Part 1. Mean serum magnesium levels increased from 1.61 (SD 0.1) on standard MG to 1.69 (SD 0.1); t(11) = 2.6, P = .02 on MGP. Five subjects completed Part 2, and all achieved serum magnesium ≥1.7 mg/dL (mean 1.75 mg/dL, SD 0.06; t(4) = 2.7, P = .06). Subjects reported the same number of, but looser bowel movements with MGP; however, individuals did not perceive intolerable GI symptoms with MGP therapy and all chose to remain on MGP at the end of the study. At an equivalent mg/kg/d dose of elemental magnesium, serum magnesium levels on MGP were significantly higher.
Genesis and Metallogenic Process of the Lujing Uranium Deposit, Southwest Jiangxi Province, China: Constraints of Micropetrography and S–C–O Isotopes
Authors: Zhang Xiao‐Tian , Pan Jia‐Yong , Xia Fei , Zhang Yong , Liu Guo‐Qi , Liu Ying , Zhong Fu‐Jun 
Published: March 2018

AbstractThe Lujing uranium deposit, located in the southeastern part of the Nanling metallogenic province, is one of the representative granite‐related hydrothermal uranium deposits in South China. Basic geology, geochemistry, and geochronology of the deposit have been extensively studied. However, there is still a chronic lack of systematic research on the genesis and metallogenic process of the deposit. Thus, we recently carried out an electron microprobe and stable isotopic analysis. The main research results and progresses are as follows:Uranium minerals in this deposit include coffinite, pitchblende, and uranothorite, and small amounts of uranium exist in accessory minerals in the form of isomorphism. Coffinite, which occurs predominantly as the pseudomorphs after pitchblende, also occurs as a primary mineral and is locally formed from the remobilization of uranium from adjacent uranium‐bearing minerals. The mineralizing fluid was originally composed of a magmatic fluid generated by late Yanshanian magmatism. The high As content of pyrite in ores may reflect the addition of meteoric water, or the formation water (or both), to the magmatic hydrothermal system. The δ34S values vary from −14.4‰ to 13.9‰ (mean δ34S = −3.9‰), showing a range that is similar to nearby Cambrian metamorphic strata and Indosinian granites, indicating that these host rocks represent the source of sulfur; however, the possibility of a mantle source cannot be completely ruled out. According to our new isotopic data and recent Pb isotopic data, we conclude that the uranium in ores was derived by leaching dominantly from the uranium‐rich host rocks, especially the Cambrian metamorphic strata. The δ13CPDB values (−8.75‰ to 1.40‰; mean δ13CPDB = −5.41‰) and δ18OSMOW values (5.45–18.62‰; mean δ18O = 13.02‰) of reddish calcite from the ore‐forming stage suggest that the CO2 in the mineralizing fluids was derived predominantly from the mantle, with a small component contributed by marine carbonates. Based on these new data and previous research results, this paper proposes that uranium metallogenesis in the Lujing deposit is closely associated with mafic magmatism resulting from crustal extension during the Cretaceous to Paleogene in South China.
Three-by-Three Basketball: Inertial Movement and Physiological Demands During Elite Games
Authors: Montgomery Paul G. , Maloney Brendan D. 
Published: March 2018

Purpose: To determine the demands of elite male and female 3 × 3 basketball games and compare these between various competition levels. Methods: A total of 361 males and 208 females competing in the Under-18 World, Senior European, and World Championships and selected professional tournaments had game demands assessed by wearable technology (global positioning system, inertial measurement, heart rate) along with postgame blood lactate and perceived responses. Differences in the means were compared using magnitude-based inferences and reported with effect size and 90% confidence limits, along with the percentage difference (effect size; ±90% confidence limits, %) of log-transformed data. Results: PlayerLoad™ and PlayerLoad·min−1 during play was 127.5 (31.1) and 6.7 (1.5) for males and 128.5 (32.0) and 6.5 (1.4) for females, respectively, with small differences between junior, senior, and professional levels. There were small differences in accelerations >3.5 m·s−1 between competition levels up to 0.31; ±0.20, 6.9% for males and 0.29; ±0.19, 10.8% for females and for decelerations >3.5 m·s−1, 0.29; ±0.19, 15.6% for males and 0.26; ±0.19, 5.4% for females, with European Championships generally greater than other levels. Average game heart rate was 165 (18) and 164 (12) beats·min−1 for males and females, with no difference between levels. Average rating of perceived exertion was 5.7 (2.1) and 5.4 (2.0) for males and females. Conclusions: 3 × 3 basketball games require high-speed inertial movements within limited distance, creating a relatively high physiological response. Practitioners working with 3 × 3 players should endeavor to focus on the attributes that will improve these player characteristics for greater success.
Effect of Intermittent Hypoxia Training for Dizziness: A Randomized Controlled Trial
Authors: Bao Xiao , Tan Jie-Wen , Long Ying , Liu Howe , Liu Hui-Yu 
Published: March 2018

Objective: To study the effect of intermittent hypoxia training (IHT) for dizziness. Design: A single-blind, randomized controlled trial. All participants were recruited from a rehabilitation department in an acute university-affiliated hospital. Intervention: Participants with dizziness were randomly assigned to 2 groups (IHT group and control group). The Dizziness Handicap Inventory, Activities-specific Balance Confidence Scale, and Vertigo Visual Analog Scale were conducted at baseline, end of the fourth week. Results: Among 52 subjects, there were18 males and 34 females, ages 35 to 62 years old (mean [SD] = 46.9 [7.93]). Time length since onset ranged from 12 to 34 months (20.2 [7.15] mo). Dizziness Handicap Inventory, Activities-specific Balance Confidence Scale, Vertigo Visual Analog Scale scores, and attack frequencies of dizziness were improved after IHT intervention in the end of the fourth week. There were significant differences between the IHT group and the control group in the Dizziness Handicap Inventory, Activities-specific Balance Confidence Scale, Vertigo Visual Analog Scale scores, and attack frequencies of dizziness at the end of the fourth week (P < .05). No adverse events occurred during the study. Conclusion: IHT could improve dizziness after intervention at the end of the fourth week. IHT could be the effective method for treating dizziness.
Pharmacokinetics of Intravenous Posaconazole in Critically Ill Patients
Authors: Sime Fekade B. , Stuart Janine , Butler Jenie , Starr Therese , Wallis Steven C. , Pandey Saurabh , Lipman Jeffrey , Roberts Jason A. 
Published: March 2018

ABSTRACT

To date, there is no information on the intravenous (i.v.) posaconazole pharmacokinetics for intensive care unit (ICU) patients. This prospective observational study aimed to describe the pharmacokinetics of a single dose of i.v. posaconazole in critically ill patients. Patients with no history of allergy to triazole antifungals and requiring systemic antifungal therapy were enrolled if they were aged ≥18 years, central venous access was available, they were not pregnant, and they had not received prior posaconazole or drugs interacting with posaconazole. A single dose of 300 mg posaconazole was administered over 90 min. Total plasma concentrations were measured from serial plasma samples collected over 48 h, using a validated chromatographic method. The pharmacokinetic data set was analyzed by noncompartmental methods. Eight patients (7 male) were enrolled with the following characteristics: median age, 46 years (interquartile range [IQR], 40 to 51 years); median weight, 68 kg (IQR, 65 to 82 kg); and median albumin concentration, 20 g/liter (IQR, 18 to 24 g/liter). Median (IQR) pharmacokinetic parameter estimates were as follows: observed maximum concentration during sampling period (
C
max
), 1,702 ng/ml (1,352 to 2,141 ng/ml); area under the concentration-time curve from zero to infinity (AUC
0–∞
), 17,932 ng · h/ml (13,823 to 27,905 ng · h/ml); clearance (CL), 16.8 liters/h (11.1 to 21.7 liters/h); and volume of distribution (
V
), 529.1 liters (352.2 to 720.6 liters). The
V
and CL were greater than 2-fold and the AUC
0–∞
was 39% of the values reported for heathy volunteers. The AUC
0–∞
was only 52% of the steady-state AUC
0–24
reported for hematology patients. The median of estimated average steady-state concentrations was 747 ng/ml (IQR, 576 to 1,163 ng/ml), which is within but close to the lower end of the previously recommended therapeutic range of 500 to 2,500 ng/ml. In conclusion, we observed different pharmacokinetics of i.v. posaconazole in this cohort of critically ill patients compared to those in healthy volunteers and hematology patients.
Influence of Reactive Oxygen Species onDe NovoAcquisition of Resistance to Bactericidal Antibiotics
Authors: Hoeksema Marloes , Brul Stanley , ter Kuile Benno H. 
Published: March 2018

ABSTRACTThe radical-based theory proposes that three major classes of bactericidal antibiotics, i.e., β-lactams, quinolones, and aminoglycosides, have in common the downstream formation of lethal levels of reactive oxygen species (ROS) as part of the killing mechanism. If bactericidal antibiotics exhibit a common mechanism, then it is to be expected that the acquisition of resistance against these drugs would have some shared traits as well. Indeed, cells made resistant to one bactericidal antibiotic more rapidly became resistant to another. This effect was absent after induced resistance to a bacteriostatic drug.De novoacquisition of resistance to one bactericidal antibiotic provided partial protection to killing by bactericidal antibiotics from a different class. This protective effect was observed in short-term experiments. No protective effect was detected during 24-h exposures, suggesting that cross-resistance did not occur. In the wild-type strain, exposure to bactericidal antibiotics increased intracellular ROS levels. This increase in ROS levels was not observed when strains resistant to these drugs were exposed to the same concentrations. These results indicate thatde novoacquisition of resistance to the bactericidal drugs tested involves a common cellular response that provides protection against ROS accumulation upon exposure to this type of antibiotics. A central mechanism or at least a few common elements within the separate mechanisms possibly play a role during the acquisition of antibiotic resistance.
CrpP Is a Novel Ciprofloxacin-Modifying Enzyme Encoded by the Pseudomonas aeruginosa pUM505 Plasmid
Authors: Chávez-Jacobo Víctor M. , Hernández-Ramírez Karen C. , Romo-Rodríguez Pamela , Pérez-Gallardo Rocío Viridiana , Campos-García Jesús , Gutiérrez-Corona J. Félix , García-Merinos Juan Pablo , Meza-Carmen Víctor , Silva-Sánchez Jesús , Ramírez-Díaz Martha I. 
Published: March 2018

ABSTRACT

The pUM505 plasmid, isolated from a clinical
Pseudomonas aeruginosa
isolate, confers resistance to ciprofloxacin (CIP) when transferred into the standard
P. aeruginosa
strain PAO1. CIP is an antibiotic of the quinolone family that is used to treat
P. aeruginosa
infections.
In silico
analysis, performed to identify CIP resistance genes, revealed that the 65-amino-acid product encoded by the
orf131
gene in pUM505 displays 40% amino acid identity to the
Mycobacterium smegmatis
aminoglycoside phosphotransferase (an enzyme that phosphorylates and inactivates aminoglycoside antibiotics). We cloned
orf131
(renamed
crpP
, for
c
iprofloxacin
r
esistance
p
rotein,
p
lasmid encoded) into the pUCP20 shuttle vector. The resulting recombinant plasmid, pUC-
crpP
, conferred resistance to CIP on
Escherichia coli
strain J53-3, suggesting that this gene encodes a protein involved in CIP resistance. Using coupled enzymatic analysis, we determined that the activity of CrpP on CIP is ATP dependent, while little activity against norfloxacin was detected, suggesting that CIP may undergo phosphorylation. Using a recombinant His-tagged CrpP protein and liquid chromatography–tandem mass spectrometry, we also showed that CIP was phosphorylated prior to its degradation. Thus, our findings demonstrate that CrpP, encoded on the pUM505 plasmid, represents a new mechanism of CIP resistance in
P. aeruginosa
, which involves phosphorylation of the antibiotic.
Cysteamine, an Endogenous Aminothiol, and Cystamine, the Disulfide Product of Oxidation, Increase Pseudomonas aeruginosa Sensitivity to Reactive Oxygen and Nitrogen Species and Potentiate Therapeutic Antibiotics against Bacterial Infection
Authors: Fraser-Pitt Douglas J. , Mercer Derry K. , Smith Daniel , Kowalczuk Aleksandra , Robertson Jennifer , Lovie Emma , Perenyi Peter , Cole Michelle , Doumith Michel , Hill Robert L. R. , Hopkins Katie L. , Woodford Neil , O'Neil Deborah A. 
Published: March 2018

ABSTRACTCysteamine is an endogenous aminothiol produced in mammalian cells as a consequence of coenzyme A metabolism through the activity of the vanin family of pantetheinase ectoenzymes. It is known to have a biological role in oxidative stress, inflammation, and cell migration. There have been several reports demonstrating anti-infective properties targeting viruses, bacteria, and even the malarial parasite. We and others have previously described broad-spectrum antimicrobial and antibiofilm activities of cysteamine. Here, we go further to demonstrate redox-dependent mechanisms of action for the compound and how its antimicrobial effects are, at least in part, due to undermining bacterial defenses against oxidative and nitrosative challenges. We demonstrate the therapeutic potentiation of antibiotic therapy againstPseudomonas aeruginosain mouse models of infection. We also demonstrate potentiation of many different classes of antibiotics against a selection of priority antibiotic-resistant pathogens, including colistin (often considered an antibiotic of last resort), and we discuss how this endogenous antimicrobial component of innate immunity has a role in infectious disease that is beginning to be explored and is not yet fully understood.
Efficacy of Cry1Ac protein against gypsy moth and fall webworm in transgenic poplar (Populus davidiana × Populus bolleana) by bioassay
Authors: Ding Liping , Chen Yajuan , Wang Hongzhi , Wei Jianhua 
Published: March 2018

A modified Bacillus thuringiensis (Bt) cry1Ac gene was introduced into poplar ‘Shanxin’ (Populus davidiana × Populus bolleana) by Agrobacterium-mediated transformation. Seventeen cry1Ac transgenic lines were regenerated. The integration and expression of the cry1Ac gene in these transgenic lines were analyzed by polymerase chain reaction (PCR) and reverse-transcription quantitative PCR, respectively. Variable amounts of cry1Ac mRNA accumulated in different transgenic plants. The expression of Cry1Ac toxin protein in transgenic plants was analyzed by enzyme-linked immunosorbent assay, the concentration ranged from 0.12 to 21.00 μg g−1. The concentration of Cry1Ac protein was highly consistent with mRNA amount in each transgenic line. Leaf section bioassays were conducted using the larvae of both gypsy moth (Lymantria dispar) and fall webworm (Hyphantria cunea). Those plants, except lines 10 and 11, caused rapid mortality of all gypsy moth and fall webworm larvae with almost no defoliation, while lines 10 and 11, with Cry1Ac protein concentrations of 0.12 and 0.76 μg g−1, respectively, provided relatively weak insect protection. Results of insect bioassays indicate that Cry1Ac protein expression over 1.3 μg g−1 consistently produced high insect mortality for both species of moths. The correlation between concentration of Cry1Ac protein and insect mortality is important to understand for managing insect pests with transgenic plants. Transgenic poplars with high insect resistance will be useful tools for managing Lepidoptera pests of these trees.
ProCMotive
Authors: Mosenia Arsalan , Bechara Jad F. , Zhang Tao , Mittal Prateek , Chiang Mung 
Published: March 2018

In recent years, numerous vehicular technologies, e.g., cruise control and steering assistant, have been proposed and deployed to improve the driving experience, passenger safety, and vehicle performance. Despite the existence of several novel vehicular applications in the literature, there still exists a significant gap between resources needed for a variety of vehicular (in particular, data-dominant, latency-sensitive, and computationally-heavy) applications and the capabilities of already-in-market vehicles. To address this gap, different smartphone-/Cloud-based approaches have been proposed that utilize the external computational/storage resources to enable new applications. However, their acceptance and application domain are still very limited due to programmability, wireless connectivity, and performance limitations, along with several security/privacy concerns.
In this paper, we present a novel reference architecture that can potentially enable rapid development of various vehicular applications while addressing shortcomings of smartphone-/Cloud-based approaches. The architecture is formed around a core component, called SmartCore, a privacy/security-friendly programmable dongle that brings general-purpose computational and storage resources to the vehicle and hosts in-vehicle applications. Based on the proposed architecture, we develop an application development framework for vehicles, that we call ProCMotive. ProCMotive enables developers to build customized vehicular applications along the Cloud-to-edge continuum, i.e., different functions of an application can be distributed across SmartCore, the user's personal devices, and the Cloud.
In order to highlight potential benefits that the framework provides, we design and develop two different vehicular applications based on ProCMotive, namely, Amber Response and Insurance Monitor. We evaluate these applications using real-world data and compare them with state-of-the-art technologies.
Intratumoral CpG-B Promotes Antitumoral Neutrophil, cDC, and T-cell Cooperation without Reprograming Tolerogenic pDC
Authors: Humbert Marion , Guery Leslie , Brighouse Dale , Lemeille Sylvain , Hugues Stephanie 
Published: March 2018

Abstract
Cancer immunotherapies utilize distinct mechanisms to harness the power of the immune system to eradicate cancer cells. Therapeutic vaccines, aimed at inducing active immune responses against an existing cancer, are highly dependent on the immunological microenvironment, where many immune cell types display high levels of plasticity and, depending on the context, promote very different immunologic outcomes. Among them, plasmacytoid dendritic cells (pDC), known to be highly immunogenic upon inflammation, are maintained in a tolerogenic state by the tumor microenvironment. Here, we report that intratumoral (i.t.) injection of established solid tumors with CpG oligonucleotides-B (CpG-B) inhibits tumor growth. Interestingly, control of tumor growth was independent of tumor-associated pDC, which remained refractory to CpG-B stimulation and whose depletion did not alter the efficacy of the treatment. Instead, tumor growth inhibition subsequent to i.t. CpG-B injection depended on the recruitment of neutrophils into the milieu, resulting in the activation of conventional dendritic cells, subsequent increased antitumor T-cell priming in draining lymph nodes, and enhanced effector T-cell infiltration in the tumor microenvironment. These results reinforce the concept that i.t. delivery of TLR9 agonists alters the tumor microenvironment by improving the antitumor activity of both innate and adaptive immune cells.
Significance: Intratumoral delivery of CpG-B disrupts the tolerogenic tumor microenvironment and inhibits tumor growth. Cancer Res; 78(12); 3280–92. ©2018 AACR.
The Circular RNA circPRKCI Promotes Tumor Growth in Lung Adenocarcinoma
Authors: Qiu Mantang , Xia Wenjia , Chen Rui , Wang Siwei , Xu Youtao , Ma Zhifei , Xu Weizhang , Zhang Erbao , Wang Jie , Fang Tian , Hu Jingwen , Dong Gaochao , Yin Rong , Wang Jun , Xu Lin 
Published: March 2018

Abstract
Somatic copy number variations (CNV) may drive cancer progression through both coding and noncoding transcripts. However, noncoding transcripts resulting from CNV are largely unknown, especially for circular RNAs. By integrating bioinformatics analyses of alerted circRNAs and focal CNV in lung adenocarcinoma, we identify a proto-oncogenic circular RNA (circPRKCI) from the 3q26.2 amplicon, one of the most frequent genomic aberrations in multiple cancers. circPRKCI was overexpressed in lung adenocarcinoma tissues, in part due to amplification of the 3q26.2 locus, and promoted proliferation and tumorigenesis of lung adenocarcinoma. circPRKCI functioned as a sponge for both miR-545 and miR-589 and abrogated their suppression of the protumorigenic transcription factor E2F7. Intratumor injection of cholesterol-conjugated siRNA specifically targeting circPRKCI inhibited tumor growth in a patient-derived lung adenocarcinoma xenograft model. In summary, circPRKCI is crucial for tumorigenesis and may serve as a potential therapeutic target in patients with lung adenocarcinoma.
Significance: These findings reveal high expression of the circular RNA circPRKCI drives lung adenocarcinoma tumorigenesis. Cancer Res; 78(11); 2839–51. ©2018 AACR.