bluuebunny/crossref_metadata_2025_split huggingface dataset:
H19/miR-194/E2F3 regulating loop promotes gastric cancer growth and metastasis
Authors: Qin Wanxiang , Shi Ying , Zhu Dan , Chen Yaohua , Li Yuping , Cui Jian 
Published: August 2019

Abstract
Background: Gastric cancer (GC) is one of the most frequent malignant digestive tumors, and second fatal cancer. This study was to investigate whether lncRNA-H19 can regulate E2F3 expression through competitive binding to microRNA-194 (miR-194), thus regulating GC growth and metastasis. Methods: H19, miR-194 and E2F3 expression levels in GC tissues and cell lines were investigated using quantitative reverse transcriptase-polymerase chain reaction (QRT-PCR). Meanwhile, the mRNA levels of H19 and E2F3 in gastric cancer tissues were also analyzed though the GEPIA web tool. The binding condition of miR-194 with H19 and E2F3 were investigated using a dual-luciferase reporter gene assay. The regulatory effects of H19 on proliferative, migratory and invasive abilities of AGS cells and SGC-7901 cells were detected by transwell assay and cell counting kit-8 (CCK-8). The regulatory interaction between H19 and miR-194, miR-194 and E2F3 were investigated using rescue experiments. Results: The results revealed that H19 was highly expressed in GC tissues and cell lines than those of controls. Downregulated H19 decreased the proliferation, migration and invasion of AGS cells and SGC-7901 cells. H19 was demonstrated that being the molecular sponge of miR-194 in regulating the growth of the GC cells. The level of E2F3 expression was also found significantly higher in GC tissues and cell lines than those of controls. And then, the mimics of miR-194 inhibited the expression of E2F3 in the GC cells. CCK-8 assay showed decreased proliferative ability induced by miR-194 mimics were reversed by E2F3 overexpression. Transwell assays showed decreased migratory and invasive ability induced by miR-194 mimics were reversed by E2F3 overexpression. Conclusions: This study demonstrates that H19 promotes GC growth and metastasis by regulating E2F3 via competitive binding to miRNA-194.
Chinese Classical Prescription(Liang-Xue-Di-Huang Decoction)for Hemorrhoidal Disease: study protocol for a randomized controlled trial
Authors: Zhou Qing , Shi Shuo-yang , He Zong-qi , Xu Cheng-biao , Geng Ji , Chen Tuo , Shen Zhao-feng , Zhang Dan , Jiang Feng , Chen Yu-Gen , Wu Ben-sheng 
Published: August 2019

Abstract
Abstract

Background: Hemorrhoidal disease (HD) is one of the commonest proctologic condition in the general population. Medical therapy for HD has not been formally confirmed due to the inconsistent of results. Liang-Xue-Di-Huang Decoction, a kind of ancient Chinese classical prescription, has been used to treat HD from the 19th century in China. However, clinical research of Liang-Xue-Di-Huang Decoction in the treatment of HD is lack. We designed this study to evaluate the efficacy and safety of Liang-Xue-Di-Huang Decoction in the treatment of HD.

Methods/Design: A randomized, controlled, double blind, double-mimetic agent and multicenter trial to evaluate the efficacy and safety of Liang-Xue-Di-Huang Decoction is proposed. HD patients (stage I, Ⅱ, Ⅲ) will be randomly assigned into Liang-Xue-Di-Huang Decoction with the addition of Diosmine mimetic agent group and Diosmine with the addition of Liang-Xue-Di-Huang Decoction mimetic agent group. Patients will receive a 7-day treatments and a 7-day follow-up. The primary outcome measure is the French Bleeding Score in 7 and 14 days. The Secondary outcome measures are Goligher Prolapse Score and Quality-of-Life Score in 7 and 14 days.

Discussion: This study will provide objective evidence to evaluate the efficacy and safety of Liang-Xue-Di-Huang Decoction in treatment of HD.

Trial registration: Chinese Clinical Trial Registry. ChiCTR-1900022531.Registered 15 Apr 2019, http://www.chictr.org.cn/listbycreater.aspx.
Community Based Essential Newborn Care Practices and Associated Factors among Women Who Gave Birth at Home in Last Twelve Months in Amaro Woreda, Southern Ethiopia, 2019
Authors: Agonafir Mamush , Shimbre Mulugeta Shegaze , Hussen Sultan , Temesgen Gebremaryam , Girmay Goytom , Tsegaye Behailu , Gebretsadik Woiynshet , Boda Belay , Fikadu Teshale 
Published: August 2019

Abstract
Introduction:- Significant numbers of women are giving birth at home; in this case community based newborn care is a means of bringing life-saving care to mothers and newborns at the community level. However, practice is challenging within the Ethiopian health system.

Objective:- The aim of this study was to assess prevalence of community based newborn care practices and its associated factors among women who gave birth at home in Amaro Woreda, southern Ethiopia, 2019.

Methods:- Across-sectional study was conducted on 490 women in the reproductive age groups of 15-49 in Amaro district and by using simple random sampling technique individual was recruited. Data collected through face-to-face interview at household level. EpiData version 3.1 statistical software was used for entry and SPSS version 20 for was used for data cleaning, management and analysis. Bi-variate and multivariate logistic regression analysis employed for analysis of factors associated with Community based newborn care practices.

Results:- A total 490 of study participants were included in the analysis and only 29% practiced community based essential newborn care. Educational status of father [AOR=2.28; 95%CI:1.07-4.84] & mother [AOR=0.35; 95%CI: 0.16-0.75], last delivery assisted by relative/friends [AOR=3.58; 95%CI: 1.66-7.73], having awareness about Community based newborn care [AOR=3.49; 95%CI: 2.11-5.77], awareness about newborn danger sign [AOR=2.18; 95%CI: 1.29-3.68] and having birth preparedness and complication readiness plan [AOR=3.52; 95%CI: 1.97-6.29] were an identified independent factors associated with Community based newborn care Practice.

Conclusion and recommendation:- Around three-fourth (71%) of mothers were not practicing Community based newborn care. Educational status of family, awareness about community based newborn care and newborn danger sign, last delivery assisted by relative or friends at home, and having birth preparedness and complication readiness plan were identified as independent factors for community based newborn care practice. Promotion of information at community level, women empowerment and health extension program strengthening are recommending.
Full Prevalence Of Gestational Diabetes In Urban Women In Blantyre, Malawi: A Cross Sectional Study And Comparison Of Who And IADPSG Criteria
Authors: Phiri Tamara Joy , Kasiya Marianne , Allain Theresa J 
Published: August 2019

Abstract
Aims This study aimed to establish the prevalence of gestational diabetes mellitus (GDM) among urban women in Blantyre where the prevalence was previously unknown. A comparison was made between the prevalence determined using WHO criteria and that using the recent International Association of Diabetes in Pregnancy Study Group (IADPSG) criteria. Methods 2274 consecutive women presenting to five antenatal clinic sites in Blantyre had a screening random blood glucose (RBG). Of these, a subsample of 250 women were randomly selected for an oral glucose tolerance test (OGTT). Logistic regression was used to quantify the association between various exposure variables and prevalence of diabetes. Results Mean age was 25 years (range 14 – 43 years), 66% were in the third trimester. Mean RBG was 5.1mmol/L (range 2.4 - 10.6). Overall prevalence of GDM was 1.6% and 24% using the WHO and IADPSG criteria respectively. GDM was associated with older maternal age, high parity and attendance at government antenatal clinics but not with MUAC, BMI, positive family history of diabetes or previous poor neonatal outcome. There was no correlation between random blood glucose and gestational diabetes diagnosed on the oral glucose tolerance test. Conclusions The prevalence of GDM in Blantyre using WHO criteria was low in the predominantly young population that was screened. A much higher proportion had GDM based on the IADPSG criteria and these may warrant long term follow up. GDM was not associated with some previously described risk factors for GDM suggesting a different risk factor profile compared to high income countries.
First referral to an Integrated Onco-Palliative Care program: a retrospective analysis of its timing
Authors: Barth Claire , Colombet Isabelle , Montheil Vincent , Huillard Olivier , Boudou-Rouquette Pascaline , Tlemsani Camille , Alexandre Jérôme , Goldwasser François , Vinant Pascale 
Published: August 2019

Abstract
Background

Palliative care (PC) referral is recommended early in the course of advanced cancer. This study aims to describe, in an integrated onco-palliative care program (IOPC), patient’s profile when first referred to this program, timing of this referral and its impact on end-of-life care.

Patients and Methods

The IOPC combined the weekly onco-palliative meeting (OPM) dedicated to patients for whom goals and organization of care need to be discussed, and/or the clinical evaluation by the PC team. We analyzed all patients first referred at OPM in 2011-2013. We defined the index of precocity (IP), as the ratio of the time from first referral to death by the time from diagnosis of incurability to death, ranging from 0 (late referral) to 1 (early referral).

Results

Of the 416 patients included, 57% presented with lung, urothelial cancers, or sarcoma. At first referral to IOPC, 76% were receiving antitumoral treatment, 63% were outpatients, 56% had a PS ≤2 and 46% had a serum albumin level >35g/l. The median [1st-3rd quartile] IP was 0.39 [0.16-0.72], ranging between 0.53 [0.20-0.79] (earliest, for lung cancer) to 0.16 [0.07-0.56] (latest, for prostate cancer). Among 367 decedents, 42 (13%) received antitumoral treatment within 14 days before death, and 157 (43%) died in PC units.

Conclusions

The IOPC is an effective organization to enable early integration of PC and decrease aggressiveness of care near the end-of life. The IP is a useful tool to model the trajectory of care while taking into account each cancer types and therapeutic advances.
Global, regional, and national prevalence of diabetes mellitus in patients with pulmonary tuberculosis: a systematic review and meta-analysis
Authors: Li Minmin , Chen Tao , Hua Zhongqiu , Zhu Ni , Wang Duolao , Li Jiamin , Li Zhaoqing , Kang yijun , Li Chao , Yan Hong 
Published: August 2019

Abstract
Background. The concept that people with pulmonary tuberculosis are at risk of developing diabetes mellitus has been raised. However, the prevalence of diabetes mellitus in patients with pulmonary tuberculosis have not been well established. We aim to estimate the global, regional, and national prevalence of diabetes mellitus in population with pulmonary tuberculosis.
Methods. In this systematic review and meta-analysis, we assessed observational studies of diabetes mellitus in people with pulmonary tuberculosis, using PubMed and Embase electronic bibliographic databases in English language, to identify articles published until August 31, 2018. We included original research studies published in a peer-reviewed journal and reported the prevalence of diabetes mellitus or had enough data to compute these estimates. Studies were excluded if they did not provide primary data or were case studies and reviews. Independent extraction of articles and collected detailed information by 2 authors using predefined questionnaire, including study quality indicators. The country-specific random-effects meta-analyses for countries with two or more available studies and a fractional response regression model to predict individual studies prevalence of diabetes mellitus in patients with pulmonary tuberculosis for countries with one or no study. The study is registered with PROSPERO, registration number CRD42018101989.
Results. We identified 18042 studies, and 127 were retained for data extraction across 46 countries. The global prevalence of diabetes mellitus in patients with pulmonary tuberculosis were estimated to be 12.07% (95%CI: 10.43-14.85). The prevalence of diabetes mellitus in patients with pulmonary tuberculosis was 13.38% (95%CI: 11.16-16.05) in region of Americas, 13.34% (95%CI: 12.82-14.61) in European region, 12.68% (95%CI: 9.15-16.37) in South-East Asia, 12.56% (95%CI: 11.79-22.70) in Western Pacific region, 10.95% (95%CI: 9.04-17.83) in Eastern Mediterranean region and 7.54% (95%CI: 6.51-8.77)in African region. The country with the highest estimated prevalence of pulmonary tuberculosis combined diabetes mellitus were Mauritius (39.65%, 95%CI: 4.22-90.74).
Conclusion. Our findings suggest that pulmonary tuberculosis combined diabetes mellitus is still prevalent. As such, diabetes mellitus deserves more attention from PTB health-care providers, researchers, policy makers, and stakeholders for improved detection, overall proper management, and efficient control of diabetes mellitus in people with pulmonary tuberculosis.
Contraceptive use and preference of HIV infected pregnant women living with HIV-negative partners in the central region of Cameroon: a cross sectional survey
Authors: Kuete Martin , Ngueye Hilary Christiane Sipeuwou , Wang Sean Zhe , Yuan HongFang , Ma XiuLan , Mandengue Christine , Ndaya Berthine Marie Miste , Nyangono Yannick Zomnyate , Zangue Carrel Raspail Founou , Xiong ChengLiang , Zhang HuiPing 
Published: August 2019

Abstract
Background

Evidences in sub-Saharan Africa including Cameroon indicate that most of HIV discordant couples want more children despite their HIV status. Investigate and establish contraception preferences among HIV infected individuals are fundamental and crucial to provide effective reproductive healthcare.Methods

We performed a cross-sectional study using structured questionnaire to explore HIV-positive pregnant women patterns including their family planning services, their preferences and its use, and their knowledge related to HIV/AIDS. Bivariate and multivariate analyses were conducted to explore associations and predictors of contraception preference and use; all tests were two sided significant at P < 0.05.Results

Overall, 94 HIV-positive pregnant women aged 30.70±5.50 years living with HIV-negative partners were from the different areas of the central region of Cameroon. Three-fourths were aware of the effectiveness of modern contraceptives and condoms, and only 28% had experienced modern contraption. 98% preferred to use traditional methods associated with infrequent condoms use. Multiple sociodemographic factors (marital status, group age, educational level, religion, occupation) affected contraceptive method preferences and its use ( P <0.05). These factors are the landmarks to predict discordant couples’ behavior in HIV disclosure, discussion and decision making for contraception, preventing mother to-child transmission and HIV-negative partner infection ( P <0.05).Conclusion

Despite the awareness of participants related both on contraception methods and HIV/AIDS matters, participants faced societal, cultural and demographic barriers to make own decision for contraception use. Promoting effective family planning services and given the entire range of contraception options may help women living with HIV to choose for effective ones and consequently reduce newly HIV infections.
Amount of maternal factor within the male pronucleus affects the reprogramming and developmental efficiency of androgenetic embryos
Authors: Liao Chen , Shen Xinghui , Zhang Yuwei , Lei Lei 
Published: August 2019

Abstract
Background

Uniparental embryos have uniparental genomes and are very useful models for studying parental specific gene expression or for exploring the biological significance of genomic imprinting in mammals. However, the early developmental efficiency of androgenetic embryos is significantly lower than that of parthenogenetic embryos. In addition, oocytes are able to reprogram the nuclei of sperm after fertilization to guarantee embryonic development by maternal derived reprogramming factors, which accumulate during oogenesis. However, importance of maternal materials in the efficiency of reprogramming the pronucleus of androgenetic embryos has not been ascertained.Results

Androgenetic embryos were constructed artificially by pronucleus transfer (PT) or double sperm injection (DS) in our experiments. Compared with the androgenetic embryos constructed artificially by DS, those constructed by PT, which derived from two zygotes, contained more maternal material (like Tet3 and H3.3). This study confirmed the better developmental potential of PT embryos, with higher blastocyst rates, the stronger expression of pluripotent genes, the lower expression of apoptotic genes, and superior blastocyst quality.Conclusions

The aggregation of more maternal materials in the paternal pronucleus facilitated the reprogramming of the paternal genome, improving embryonic development in pronucleus transfer androgenesis.
Virtual System can be considered interchangeable with shuttle run in healthy children: a cross-sectional study
Authors: moran cristiane , CORSO SIMONE DAL , BOMBIG MARIA TERESA , SERRA ANDREY JORGE , PEREIRA SILVANA ALVES , PECCIN MARIA STELLA 
Published: August 2019

Abstract
Background

Previous studies have evaluated the physiological responses to video game in children with different clinical conditions, however, although no study has compared video game active with an incremental field test in healthy children. The purpose of this study was to compare the maximum heart rate (HRmax) of children, their perceived exertion and motivation during the shuttle run test (SRT) and the Virtual Systems (VS) test, an interactive video game.Methods

This is a cross-sectional study of 235 children (9.0 ± 0.8 years, 109 boys). The two tests were performed one week apart and the children were instructed not to engage in any physical exercise or sports in the 24 hours preceding each test. Their heart rate was monitored at rest for one minute and then throughout the tests. To evaluate the influence of motivation on test execution (SRT and VS), at the end of the tests the children were asked to rate their motivation based on a scale from zero to 10, zero being “not cool” and 10 “awesome”. Perceived exertion at the end of the tests was assessed using the modified Borg scale.Results

HRmax did not differ between the SRT and VS (194.4 ± 10.2 bpm vs. 193.2 ± 13.8 bpm, respectively). Both tests were similar in intensity > and < 96% HRmax. The children showed greater exertion on the Borg scale and motivation during the VS. The multiple logistic regression model showed that motivation (p=0.98), sex (p=0.53), age (p=0.61), nutritional status (p=0.65), and speed (p=0.18) were not predictive factors of whether the child would reach HRmax.Conclusion

The VS can be used as a tool to evaluate the intensity of maximal exercise tests given that the percentage of children who achieved HRmax did not differ between the VS and SRT. The perceived exertion scales were correlated, but only the modified Borg scale correlated with HRmax in the SRT. The tests are motivational, and most children obtained the maximum score in the VS
Process Evaluation of Implementation Fidelity of the Integrated Chronic Disease Management Model in two districts, South Africa
Authors: Lebina Limakatso , Alaba Olufunke , Ringane Ashley , Hlongwane Khuthadzo , Pule Pogiso , Oni Tolu , Kawonga Mary 
Published: August 2019

Abstract
Background

The Integrated Chronic Disease Management (ICDM) model has been implemented in South Africa to enhance efficiency and quality of clinical services in Primary Healthcare (PHC) clinics in a context of a high prevalence of chronic conditions and multi-morbidity. This study aimed to assess implementation fidelity (adherence to guidelines) of the ICDM model.Methods

A cross-sectional study in sixteen PHC clinics in two health districts in South Africa: Dr Kenneth Kaunda (DKK) and West Rand (WR). A fidelity assessment tool with 89 activities and a total maximum score of 158, was developed from the four interrelated ICDM model components: facility re-organization, clinical supportive management, assisted self-management and strengthening of support systems. Value stream mapping of patient flow was used to analyze waiting time and identify areas of inefficiencies. ICDM items were scored based on structured observations, records reviews and interviews with healthcare workers. Fidelity scores were summarized using medians and proportions and compared by facilities and districts using Chi-Square and Kruskal Wallis test.Results

The monthly patient headcount over a six-months in these 16 PHC clinics was a median of 2430 (IQR: 1685-2942) individuals over 20 years. DKK district had more newly diagnosed TB patients per month [median 5.5 (IQR: 4.00-9.33) vs 2.0 (IQR: 1.67-2.92)], and fewer medical officers per clinic [median 1 (IQR: 1-1) vs 3.5 (IQR:2-4.5)] compared to WR district. The median fidelity scores in both districts for facility re-organization, clinical supportive management, assisted self-management and strengthening of support systems were 78% [29/37, IQR: 27-31)]; 77% [30/39 (IQR: 27-34)]; 77% [30/39 (IQR: 28-34)]; and 80% [35/44 (IQR: 30-37)], respectively. The overall median implementation fidelity of the ICDM model was 79% (125/158, IQR: 117-132); WR was 80% (126/158, IQR: 123-132) while DKK was 74% (117/158, IQR: 106-130), p=0.1409. The lowest clinic fidelity score was 66% (104/158) while the highest was 86% (136/158). Patient flow analysis showed long (2-5 hours) waiting times and one stream of care for acute and chronic services.Conclusion

There was some variability of scores on components of the ICDM model by PHC clinics. More research is needed on contextual adaptations of the model.
Investigation on the satisfaction degree of scientific research environment of leprosy prevention and control personnel in China
Authors: Zhou Guojiang , Li Chengyue , Wang Jingquan , Zhang Guocheng , Sun Hong , Law Chi Kin 
Published: August 2019

Abstract
Summary Objective: To understand the situation of scientific research environment of leprosy prevention and control personnel in China, and to provide policy basis for improving its scientific research conditions, improving the scientific research ability of leprosy prevention and control, and then improving the prevention and treatment level of leprosy. Methods Through multi-stage stratified random sampling, 95 organizations and 351 leprosy prevention and control personnel were recruited. by using homemade organization interview questionnaire and scientific research environment satisfaction questionnaire. Results The overall satisfaction of the scientific research environment of leprosy prevention and control personnel in china was low (14.6%). Single factor analysis shows that the satisfaction of leprosy epidemic areas category IIwith the use of scientific research funds and the difficulty of applying for the research projects is high;and the satisfaction of scientific research at the county level and below institutions is higher than that of provincial and ministerial institutions;and the standard of scientific research award of the skin disease hospitals (Institutes) is higher than that of the CDCs. The respondents considered that the main problems at present were poor scientific research conditions, lack of scientific research and academic atmosphere, and respondents generally agreed that the construction of scientific research infrastructure was the prioritize issue that needed to be solved urgently at present. Conclusion The satisfaction of the current research environment of leprosy prevention and treatment personnel in China is not high. The construction of scientific research infrastructure and scientific research technology platform, as well as the introduction, training and reform of compensation system of leprosy prevention and treatment talents, need to be improved.