bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fabrication of single gel with different mechanical stiffness using three‐dimensional mold
Authors: Kazi Gulsan Ara Sathi , Rahman Kazi Anisur , Farahat Mahmoud , Matsumoto Takuya 
Published: May 2018

AbstractThe hydrogel is considering as functional substrates for three‐dimensional (3D) environment mimicking the native tissue in vitro. To get the cell or tissue culture result in different stiffness, researchers used separate gel at different times. Sometimes these results are manipulated by surrounding environment. To overcome this, we prepared a single hydrogel with different young modulus using a 3 D printed mold and cell culture and tissue culture was performed to check the functional capacity. In this proposed device we successfully produced a multiproperties agarose hydrogel on a single platform. We designed different mold pattern to confirm that this gel formation technique can be used for any types of design and many different concentrated hydrogels can be combined together. The cell and tissue culture results showed that even in same planer surface, each gel solely maintains their own physical properties and control the cell and tissue adherence and proliferation behavior. The protocol is fairly simple and reproducible. The design helps to produce consistent gel thickness, shape, and size. This 3 D mold has provided a new way tostudy the fundamental cellular responses to engineered microenvironments that may have a high implementation in both biological and healthcare‐related applications. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 6–11, 2019.
LncRNA MEG3 repressed malignant melanoma progression via inactivating Wnt signaling pathway
Authors: Li Peng , Gao Ying , Li Jun , Zhou Yu , Yuan Jing , Guan Huiwen , Yao Peng 
Published: May 2018

AbstractAccumulating evidence has indicated that MEG3 can serve as a tumor suppressive lncRNA in various tumors. It is aberrantly expressed in multiple cancers. However, the biological roles of MEG3 in melanoma are poorly understood. Therefore, in our study, we concentrated on the biological mechanism of MEG3 in melanoma progression. First, we observed that MEG3 was obviously decreased in melanoma cells including A375, SK‐MEL‐1, B16, and A2058 cells compared to human epidermal melanocytes HEMa‐LP. MEG3 was restored by transfecting LV‐MEG3 in to A375 and A2058 cells. Subsequently, we found that overexpression of MEG3 was able to inhibit cell proliferation and colony formation capacity. Meanwhile, melanoma cell apoptosis was induced by up‐regulation of MEG3. Overexpression of MEG3 greatly repressed melanoma cell migration and invasion ability. In addition, Wnt signaling pathway has been identified in the progression of various cancers. Here, in our study, it was indicated that Wnt signaling was highly activated in melanoma cells with β‐catenin expression significantly increased and GSK‐3β decreased. Interestingly, MEG restoration strongly inactivated Wnt signaling pathway by reducing β‐catenin and CyclinD1, elevating GSK‐3β levels in vitro. Finally, in vivo experiments were carried out to confirm the inhibitory roles of MEG3 in vivo. Taken these together, we suggested that MEG3 can inhibit melanoma development through blocking Wnt signaling pathway.
Attachment‐based interventions for families with young children
Authors: Woodhouse Susan S. 
Published: May 2018

AbstractGiven a large body of research indicating links between child attachment and later mental health outcomes, interventions that promote children's secure attachment to their caregivers have the potential to contribute to prevention of psychopathology and promotion of well‐being. A number of attachment‐based interventions have been developed to support parents, enhance caregiving quality, and promote children's attachment security with the aim of improving children's mental health. There is now a growing evidence base to support the efficacy of a number of these interventions. The present literature review and introduction to the special issue on attachment‐based interventions for families with young children describes key aspects of attachment theory and research that form the theoretical and empirical background for attachment‐based interventions, and introduces seven case studies illustrating five different attachment‐based interventions. The case studies represent a variety of attachment‐based models, including both group and individual treatments, and show applications across a range of caregiver contexts.
Family social capital mediates the effect of poverty on children's anxiety and depression
Authors: Li Chunkai , Liang Zurong , Yin Xican , Zhang Qiunv 
Published: May 2018

AbstractThis study aims to examine how family social capital mediates the relationship between poverty and children's anxiety and depression in mainland China. Data were collected with multistage cluster random sampling method in Xiushui City in mainland China. The sample comprised 1,314 school‐aged children (Grades 4–9). Data included children's basic demographics, internalizing symptoms, and parenting process. Structural equation modeling was used to test the hypothesized model. The results demonstrated a good fit for the sample. The overall model explained 12.7% and 16.1% of the variance for children's anxiety and depression, respectively. Poverty had a significantly direct effect on children's anxiety and depression, and family social capital could partially mediate the influence of poverty on children's internalizing symptoms. Family social capital plays an important mediating role between poverty and children's mental health. Our findings provide strong evidence for social work intervention. Family social capital is essential for parent–child interactions, especially in underprivileged families.
Validation of an abbreviated Sociopolitical Control Scale for Youth among a sample of underresourced urban youth of color
Authors: Lardier David T. , Reid Robert J. , Garcia‐Reid Pauline 
Published: May 2018

AbstractEmpowerment is a higher order multilevel framework that is used to understand and evaluate individuals, groups, organizations, and communities as they engage in the practice and execution of the participatory process. The intrapersonal component of psychological empowerment has been examined through sociopolitical control and occupies two dimensions: leadership competence and policy control. Though the Sociopolitical Control Scale for Youth (SPCS‐Y) has been examined using a 17‐item scale, Christens, Krauss, and Zeldin (2016) recently assessed the factorial validity of an abbreviated SPCS‐Y among a sample of Malaysian adolescents. Yet, there is a need to further examine this abbreviated SPCS‐Y among a sample of U.S adolescents. This study tested the factor structure of the abbreviated SPCS‐Y among a sample of urban youth of color (N = 383). Using multivariate analysis of variance (MANOVA) we examined the relationship leadership competence and policy control had with conceptually related variables. Analyses supported the bidimensional factor structure and the factorial validity of the abbreviated SPCS‐Y. MANOVA results also indicate that participants with both higher leadership competence and policy control also had higher composite scores among conceptually related variables.
The development of the Systems and Individual Responsibility for Poverty (SIRP) Scale
Authors: Shor Rachel , Calton Jenna M. , Cattaneo Lauren B. 
Published: May 2018

AbstractPoverty is a widespread social problem that affects a substantial number of Americans each year. Attitudes can affect a range of judgments, behavioral intentions, and actions related to addressing this problem. However, existing tools that measure attitudes toward those in poverty do not fully capture the deficit ideology that is a critical component of beliefs about social class. We developed the Systems and Individual Responsibility for Poverty (SIRP) Scale to address this gap. This article describes the development of an initial pool of 20 items, and then the refinement and validation of the final 17‐item measure. Exploratory factor analysis (N = 260) and confirmatory factor analysis (N = 280) yielded 2 factors: Individual Responsibility and Systems Responsibility. The Individual Responsibility factor comprises two subscales, differentiating between reasons for getting into and not getting out of poverty. The SIRP is a new tool for evaluating community psychologists’ efforts to shift deficit‐oriented thinking about poverty, and is likely to be particularly useful in education contexts. In future work, community psychologists might adapt the measure to assess deficit oriented thinking about other problems, and to explore how such attitudes are related to behavior.
Cavities shield birds from effects of artificial light at night on sleep
Authors: Raap Thomas , Pinxten Rianne , Eens Marcel 
Published: May 2018

AbstractLight pollution is an ever increasing worldwide problem disrupting animal behavior. Artificial light at night (ALAN) has been shown to affect sleep in wild birds. Even cavity‐nesting bird species may be affected when sleeping inside their cavity. Correlational studies suggest that light from outside the cavity/nest box, for example from street lights, may affect sleep. We used an experimental design to study to what extent nest boxes shield animals from effects of ALAN on sleep. We recorded individual sleep behavior of free‐living great tits (Parus major) that were roosting in dark nest boxes and exposed their nest box entrance to ALAN the following night (1.6 lux white LED light; a similar light intensity as was found at nest boxes near street lights). Their behavior was compared to that of control birds sleeping in dark nest boxes on both nights. Our experimental treatment did not affect sleep behavior. Sleep behavior of birds in the control group did not differ from that of individuals in the light treated group. Our results suggest that during winter cavities shield birds from some effects of ALAN. Furthermore, given that effects of ALAN and exposure to artificial light are species‐, sex‐, and season‐dependent, it is important that studies using wild animals quantify individual exposure to light pollution, and be cautious in the interpretation and generalization of the effects, or lack thereof, from light pollution. Rigorous studies are necessary to examine individual light exposure and its consequences in cavity‐ and open‐nesting birds.
Developmental plasticity in reptiles: Insights from temperature‐dependent gene expression in wall lizard embryos
Authors: Feiner Nathalie , Rago Alfredo , While Geoffrey M. , Uller Tobias 
Published: May 2018

AbstractMany features of the development of reptiles are affected by temperature, but very little is known about how incubation temperature affects gene expression. Here, we provide a detailed case study of gene expression profiles in common wall lizard (Podarcis muralis) embryos developing at stressfully low (15°C) versus benign (24°C) temperature. For maximum comparability between the two temperature regimes, we selected a precise developmental stage early in embryogenesis defined by the number of somites. We used a split‐clutch design and lizards from four different populations to evaluate the robustness of temperature‐responsive gene expression profiles. Embryos incubated at stressfully low incubation temperature expressed on average 20% less total RNA than those incubated at benign temperatures, presumably reflecting lower rates of transcription at cool temperature. After normalizing for differences in total amounts of input RNA, we find that approximately 50% of all transcripts show significant expression differences between the two incubation temperatures. Transcripts with the most extreme changes in expression profiles are associated with transcriptional and translational regulation and chromatin remodeling, suggesting possible epigenetic mechanisms underlying acclimation of early embryos to cool temperature. We discuss our findings in light of current advances in the use of transcriptomic data to study how individuals acclimatize and populations adapt to thermal stress.
Egr2 mediates the differentiation of mouse uterine stromal cells responsiveness to HB‐EGF during decidualization
Authors: Yue Liang , Yu Hai‐Fan , Yang Zhan‐Qing , Tian Xue‐Chao , Zheng Lian‐Wen , Guo Bin 
Published: May 2018

AbstractAlthough Egr2 is involved in regulating the folliculogenesis and ovulation, there is almost no data describing its physiological function in embryo implantation and decidualization. Here, we showed that Egr2 mRNA was distinctly accumulated in subluminal stromal cells around implanting blastocyst on day 5 of pregnancy as well as in estrogen‐activated implantation uterus. Estrogen induced the expression of Egr2 in uterine epithelia. Elevated expression of Egr2 mRNA was also observed in the decidual cells. Silencing of Egr2 by specific siRNA weakened the proliferation of uterine stromal cells and reduced the expression of Ccnd1, Ccnd3, Cdk4, and Cdk6. Furthermore, Egr2 advanced the expression of Prl8a2, Prl3c1, and Pgr, the well‐established differentiation markers for decidualization. Administration of exogenous recombinant heparin‐binding EGF‐like growth factor (rHB‐EGF) to uterine stromal cells resulted in an increase in the level of Egr2 mRNA. Moreover, siRNA‐mediated attenuation of Egr2 impeded the stimulation of HB‐EGF on stromal cell differentiation. Knockdown of Egr2 led to a reduction in the expression of Cox‐2, mPGES‐1, Vegf, Trp53, and Mmp2. Further analysis found that Egr2 may serve as an intermediate to mediate the regulation of HB‐EGF on Cox‐2, mPGES‐1, Vegf, Trp53, Mmp2, and Ccnd3. Collectively, Egr2 may play an important role during embryo implantation and decidualization.
Synthesis and Antibacterial Evaluation of 1,2,3‐Triazole‐based Quinazolines Using Click Chemistry in the Presence of Salophen Schiff Base Ligand
Authors: Kazemi Shaghayegh Sadat , Keivanloo Ali , Nasr‐Isfahani Hossein , Amin Amir Hossein 
Published: May 2018

The reaction of 2‐chloro‐4‐(prop‐2‐ynyloxy)quinazoline or 2‐chloro‐N‐(prop‐2‐ynyl)quinazolin‐4‐amine with aromatic azides in the presence of the salophen Schiff base ligand afforded new derivatives of 1,2,3‐triazole‐based quinazoline scaffold in high‐to‐excellent reaction yields. The main advantage of this procedure is that the toxicity of the copper catalyst used could be decreased to 2 mol% by complexation with the salophen ligand. All the synthesized compounds were screened for their in vitro antibacterial activities against the Gram‐positive and Gram‐negative bacteria using the well‐diffusion method.
Perceived organizational obstruction: A mediator that addresses source–target misalignment between abusive supervision and OCBs
Authors: Mackey Jeremy D. , McAllister Charn P. , Brees Jeremy R. , Huang Lei , Carson Jack E. 
Published: May 2018

SummaryWe examine perceived organizational obstruction as a mediator in the relationship between abusive supervision and subordinates' organizational citizenship behaviors directed toward organizations. We seek to provide a nuanced understanding of why subordinates who perceive supervisory mistreatment would target organizations with behavioral responses. Specifically, we study the implications of examining relationships between inconsistent sources of social exchange perceptions (e.g., supervisory perceptions) and targets of social exchange behaviors (e.g., organizational responses), which we refer to as social exchange source–target misalignment. Results from 3 studies (Study 1: n = 109; Study 2: n = 213; Study 3: n = 228) demonstrate evidence that abusive supervision is indirectly and negatively associated with organizational citizenship behaviors directed toward organizations through perceived organizational obstruction and that this conditional indirect effect is stronger for subordinates who perceive higher levels of supervisor organizational embodiment than others. Examining the social exchange tandem of perceived organizational obstruction and supervisor organizational embodiment provides a novel and useful means of aligning sources and targets of negative social exchange relationships across subordinates, supervisors, and organizations in order to advance our understanding of the social exchange antecedents and consequences of perceived organizational obstruction.
Quality perception, purchase intention, and the impact of information on the evaluation of refined Pacific cupped oysters (Crassostrea gigas) by Dutch consumers
Authors: van Houcke Jasper , Altintzoglou Themistokilis , Linssen Jozef , Luten Joop 
Published: May 2018

AbstractBACKGROUNDOyster refinement using land‐based pond systems is a new activity in the Dutch oyster sector. It increases the oyster's tissue weight and changes its sensorial properties. However, the response of Dutch consumers towards refined oysters is unknown. The research aim was to gain insight into the importance of oyster quality parameters, drivers for oyster consumption, and acceptance of refined oysters by Dutch consumers, taking into account the information given to them about the product and process.RESULTSTaste, texture, and odor are the most important oyster quality characteristics for Dutch consumers. The outcome of questionnaires showed that willingness to buy and pay is influenced by factors such as the oysters' country of origin, cultivation area, and flavor profile. Refinement did not affect willingness to buy and pay. Furthermore Dutch consumers seem to have a preference for the flavor profile of refined oysters. Consumer evaluation showed that refined Pacific cupped oysters were perceived as sweeter compared with non‐refined oysters. When information on the cultivation process was disclosed, overall appreciation of refined oysters by consumers increased.CONCLUSIONNew insights in the importance of oyster quality characteristics for Dutch consumers are generated that can be used in the development of refined Pacific cupped oysters. © 2018 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
Inhibiting tumor necrosis factor‐alpha at time of induced intervertebral disc injury limits long‐term pain and degeneration in a rat model
Authors: Evashwick‐Rogler Thomas W. , Lai Alon , Watanabe Hironobu , Salandra Jonathan M. , Winkelstein Beth A. , Cho Samuel K. , Hecht Andrew C. , Iatridis James C. 
Published: May 2018

BackgroundPainful intervertebral disc (IVD) degeneration has tremendous societal costs and few effective therapies. Intradiscal tumor necrosis factor‐alpha (TNFα) is commonly associated with low back pain, but the direct relationship remains unclear.PurposeTreatment strategies for low back pain require improved understanding of the complex relationships between pain, intradiscal pro‐inflammatory cytokines, and structural IVD degeneration. A rat in vivo lumbar IVD puncture model was used to 1) determine the role of TNFα in initiating painful IVD degeneration, and 2) identify statistical relationships between painful behavior, IVD degeneration, and intradiscal pro‐inflammatory cytokine expression.MethodsLumbar IVDs were punctured anteriorly and injected with TNFα, anti‐TNFα, or saline and compared with sham and naive controls. Hindpaw mechanical hyperalgesia was assayed weekly to determine pain over time. 6‐weeks post‐surgery, animals were sacrificed, and IVD degeneration, IVD height, and intradiscal TNFα and interleukin‐1 beta (IL‐1β) expressions were assayed.ResultsIntradiscal TNFα injection increased pain and IVD degeneration whereas anti‐TNFα alleviated pain to sham level. Multivariate step‐wise linear regression identified pain threshold was predicted by IVD degeneration and intradiscal TNFα expression. Pain threshold was also linearly associated with IVD height loss and IL‐1β.DiscussionThe significant associations between IVD degeneration, height loss, inflammation, and painful behavior highlight the multifactorial nature of painful IVD degeneration and the challenges to diagnose and treat a specific underlying factor. We concluded that TNFα is an initiator of painful IVD degeneration and its early inhibition can mitigate pain and degeneration. Intradiscal TNFα inhibition following IVD injury may warrant investigation for its potential to alter downstream painful IVD degeneration processes.
Thyroid Microcalcifications in the Absence of Identifiable Nodules and Their Association With Thyroid Cancer
Authors: Whittle Carolina , García Marisol , Horvath Eleonora , Slater Jeannie , Carrasco Carmen 
Published: May 2018

ObjectivesTo determine ultrasound (US) and clinical findings of thyroid microcalcifications in the absence of a nodule and their association with the risk of malignancy.MethodsThe Institutional Review Board approved a 5‐year retrospective study. Twenty‐one patients with clustered or scattered thyroid microcalcifications in the absence of nodules on US images who underwent fine‐needle aspiration biopsies (FNABs) were included. Demographic and clinical data, US findings, and pathologic results were registered. Patients with a suspicion of malignancy or papillary thyroid carcinoma (PTC) on FNAB underwent thyroidectomy. Patients with benign results on FNAB underwent clinical and US surveillance.ResultsThe mean age of the 21 patients was 33.2 years (29.5 years in patients with PTC patients and 39.4 years in those with benign findings; P = .034). Eleven of 21 patients had clustered microcalcifications (9 had cancer), and 10 of 21 patients had scattered microcalcifications (4 of 10 had cancer; P = 0.063). Sixty‐two percent of the patients had FNAB findings that were suspicious for cancer or had a diagnosis of cancer. Eleven of 13 patients had surgical thyroidectomy performed in our institution; in all cases, Hashimoto thyroiditis was confirmed. Univariate and multivariate analysis showed that only age was significant (odds ratio, 0.9; P < .05).ConclusionsOur study suggests that the presence of thyroid microcalcifications without a nodule is suspicious for PTC. We found that both patterns were suspicious for PTC, particularly in young patients. Special concern arises for those clustered microcalcifications on a background of Hashimoto thyroiditis.
A Comparison of 1‐ and 3.2‐MHz Low‐Intensity Pulsed Ultrasound on Osteogenesis on Porous Titanium Alloy Scaffolds: An In Vitro and In Vivo Study
Authors: Feng Lifang , Liu Xiaohan , Cao Hongjuan , Qin Limei , Hou Wentao , Wu Lin 
Published: May 2018

ObjectivesLow‐intensity pulsed ultrasound (LIPUS) combined with porous scaffolds can be used as a new therapy to treat bone defect repair. The aim of this study was to evaluate the effects of 1 and 3.2 MHz LIPUS on osteogenesis on porous Ti64 alloy scaffolds for both in vitro and in vivo studies.MethodsScaffolds were randomly divided into the high‐frequency ultrasound group, low‐frequency ultrasound group, and control group. Mouse pre‐osteoblast cells were cultured with porous Ti‐6Al‐4V scaffolds in vitro to evaluate cell proliferation and differentiation. In addition, scaffolds were implanted into rabbit mandibular defects in vivo. The effects of LIPUS on bone regeneration were evaluated by observing the micro–computed tomography (micro‐CT), toluidine blue staining, and von Kossa staining.ResultsThe results revealed no significant difference in the cell counting kit‐8 values between the ultrasound groups and control groups (P > .05). Compared with the control group, ultrasound promoted alkaline phosphatase activity and osteocalcin levels of the cells on the scaffolds (P < .05), but there was no significant difference between the two frequencies. In addition, histomorphologic analyses revealed that the volume and amount of new bone formation increased and that bone maturity improved in the ultrasound groups compared with the control group, but no significant difference was noted between the two frequencies.ConclusionsUnder the present experimental conditions, LIPUS promoted osteoblast differentiation and promoted bone maturity on porous Ti64 scaffolds. No significant differences were noted between the two frequencies.