bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mouse acute leukemia develops independent of self-renewal and differentiation potentials in hematopoietic stem and progenitor cells
Authors: Dong Fang , Bai Haitao , Wang Xiaofang , Zhang Shanshan , Wang Zhao , Xie Miner , Zhang Sen , Wang Jinhong , Hao Sha , Cheng Tao , Ema Hideo 
Published: February 2019

AbstractThe cell of origin, defined as the normal cell in which the transformation event first occurs, is poorly identified in leukemia, despite its importance in understanding of leukemogenesis and improving leukemia therapy. Although hematopoietic stem cells (HSCs) and hematopoietic progenitor cells (HPCs) were used for leukemia models, whether their self-renewal and differentiation potentials influence the initiation and development of leukemia is largely unknown. In this study, the self-renewal and differentiation potentials in 2 distinct types of HSCs (HSC1 [CD150+CD41−CD34−Lineage−Sca-1+c-Kit+ cells] and HSC2 [CD150−CD41−CD34−Lineage−Sca-1+c-Kit+ cells]) and 3 distinct types of HPCs (HPC1 [CD150+CD41+CD34−Lineage−Sca-1+c-Kit+ cells], HPC2 [CD150+CD41+CD34+Lineage−Sca-1+c-Kit+ cells], and HPC3 [CD150−CD41−CD34+Lineage−Sca-1+c-Kit+ cells]) were isolated from adult mouse bone marrow, and examined by competitive repopulation assay. Then, cells from each population were retrovirally transduced to initiate MLL-AF9 acute myelogenous leukemia (AML) and the intracellular domain of NOTCH-1 T-cell acute lymphoblastic leukemia (T-ALL). AML and T-ALL similarly developed from all HSC and HPC populations, suggesting multiple cellular origins of leukemia. New leukemic stem cells (LSCs) were also identified in these AML and T-ALL models. Notably, switching between immunophenotypical immature and mature LSCs was observed, suggesting that heterogeneous LSCs play a role in the expansion and maintenance of leukemia. Based on this mouse model study, we propose that acute leukemia arises from multiple cells of origin independent of the self-renewal and differentiation potentials in hematopoietic stem and progenitor cells and is amplified by LSC switchover.
Development of a prognostic model of respiratory insufficiency or death in amyotrophic lateral sclerosis
Authors: Ackrivo Jason , Hansen-Flaschen John , Wileyto E. Paul , Schwab Richard J. , Elman Lauren , Kawut Steven M. 
Published: February 2019

A clinically useful model to prognose onset of respiratory insufficiency in amyotrophic lateral sclerosis (ALS) would inform disease interventions, communication and clinical trial design. We aimed to derive and validate a clinical prognostic model for respiratory insufficiency within 6 months of presentation to an outpatient ALS clinic.We used multivariable logistic regression and internal cross-validation to derive a clinical prognostic model using a single-centre cohort of 765 ALS patients who presented between 2006 and 2015. External validation was performed using the multicentre Pooled Resource Open-Access ALS Clinical Trials (PRO-ACT) database with 7083 ALS patients. Predictors included baseline characteristics at first outpatient visit. The primary outcome was respiratory insufficiency within 6 months, defined by initiation of noninvasive ventilation, forced vital capacity (FVC) <50% predicted, tracheostomy, or death.Of 765 patients in our centre, 300 (39%) had respiratory insufficiency or death within 6 months. Six baseline characteristics (diagnosis age, delay between symptom onset and diagnosis, FVC, symptom onset site, amyotrophic lateral sclerosis functional rating scale-revised (ALSFRS-R) total score and ALSFRS-R dyspnoea score) were used to prognose the risk of the primary outcome. The derivation cohort c-statistic was 0.86 (95% CI 0.84–0.89) and internal cross-validation produced a c-statistic of 0.86 (95% CI 0.85–0.87). External validation of the model using the PRO-ACT cohort produced a c-statistic of 0.74 (95% CI 0.72–0.75).We derived and externally validated a clinical prognostic rule for respiratory insufficiency in ALS. Future studies should investigate interventions on equivalent high-risk patients.
Postnatal liver functional maturation requires Cnot complex-mediated decay of mRNAs encoding cell cycle and immature liver genes
Authors: Suzuki Toru , Kikuguchi Chisato , Nishijima Saori , Nagashima Takeshi , Takahashi Akinori , Okada Mariko , Yamamoto Tadashi 
Published: February 2019

ABSTRACT
Liver development involves dramatic gene expression changes mediated by transcriptional and post-transcriptional control. Here, we show that the Cnot deadenylase complex plays a crucial role in liver functional maturation. The Cnot3 gene encodes an essential subunit of the Cnot complex. Mice lacking Cnot3 in liver have reduced body and liver masses, and they display anemia and severe liver damage. Histological analyses indicate that Cnot3-deficient (Cnot3−/−) hepatocytes are irregular in size and morphology, resulting in formation of abnormal sinusoids. We observe hepatocyte death, increased abundance of mitotic and mononucleate hepatocytes, and inflammation. Cnot3−/− livers show increased expression of immune response-related, cell cycle-regulating and immature liver genes, while many genes relevant to liver functions, such as oxidation-reduction, lipid metabolism and mitochondrial function, decrease, indicating impaired liver functional maturation. Highly expressed mRNAs possess elongated poly(A) tails and are stabilized in Cnot3−/− livers, concomitant with an increase of the proteins they encode. In contrast, transcription of liver function-related mRNAs was lower in Cnot3−/− livers. We detect efficient suppression of Cnot3 protein postnatally, demonstrating the crucial contribution of mRNA decay to postnatal liver functional maturation.
Sound localization behavior inDrosophila melanogasterdepends on inter-antenna vibration amplitude comparisons
Authors: Batchelor Alexandra V. , Wilson Rachel I. 
Published: February 2019

ABSTRACTDrosophila melanogaster hear with their antennae: sound evokes vibration of the distal antennal segment, and this vibration is transduced by specialized mechanoreceptor cells. The left and right antennae vibrate preferentially in response to sounds arising from different azimuthal angles. Therefore, by comparing signals from the two antennae, it should be possible to obtain information about the azimuthal angle of a sound source. However, behavioral evidence of sound localization has not been reported in Drosophila. Here, we show that walking D. melanogaster do indeed turn in response to lateralized sounds. We confirm that this behavior is evoked by vibrations of the distal antennal segment. The rule for turning is different for sounds arriving from different locations: flies turn toward sounds in their front hemifield, but they turn away from sounds in their rear hemifield, and they do not turn at all in response to sounds from 90 or −90 deg. All of these findings can be explained by a simple rule: the fly steers away from the antenna with the larger vibration amplitude. Finally, we show that these behaviors generalize to sound stimuli with diverse spectro-temporal features, and that these behaviors are found in both sexes. Our findings demonstrate the behavioral relevance of the antenna's directional tuning properties. They also pave the way for investigating the neural implementation of sound localization, as well as the potential roles of sound-guided steering in courtship and exploration.
Apoptosis induction on human breast cancer T47D cell line by extracts of Ancorina sp.
Authors: Tunjung Woro Anindito Sri , Sayekti Puspa Restu 
Published: February 2019

Background:Breast cancer is the second leading cause of death in women. Alternative medicine with high efficacy is needed for breast cancer treatments, for example induction of apoptosis using natural products. It has been found that many natural apoptosis-inducing compounds are isolated from marine sponge. The objective of this study is to analyze the ability of extracts of the spongeAncorinasp. to induce apoptosis on human breast cancer T47D cell line and find out its mechanism.Methods:T47D cells were treated with crude extracts of methanol, dichloromethane:methanol (1:1) and dichloromethaneAncorinasp. for 24 h, and doxorubicin was used as a positive control. Methods used for this study were MTT assay to examine cell viability and determine IC50of the three extracts, while the percentage of apoptosis and caspase-3 were investigated by flow cytometry.Results:IC50values of methanol, dichloromethane:methanol (1:1), and dichloromethane extract were 84.25, 121.45, and 99.85μg/mL respectively. The percentages of apoptotic cells after treatment with methanol, dichloromethane:methanol (1:1), and dichloromethane extracts were 88.68, 27.54 and 53.63% respectively, whereas the percentage of caspase-3 was 77.87, 12.66 and 12.97%, respectively.Conclusions:These results revealed that all extracts ofAncorinasp. have strong or moderate cytotoxicity and have the ability to induce apoptosis on T47D human breast cancer cell line. However, methanol crude extract has high efficacy to induce apoptosis through caspase-3 activation compared to the other extracts. Hence methanol extract warrants further investigation as a natural medicine for human breast cancer.
The challenge of differentiating vaso-occlusive crises from osteomyelitis in children with sickle cell disease and bone pain: A 15-year retrospective review
Authors: Fontalis A. , Hughes K. , Nguyen M. P. , Williamson M. , Yeo A. , Lui D. , Gelfer Y. 
Published: February 2019

Purpose The paediatric sickle cell disease (SCD) osteomyelitis (OM) incidence is 0.3% to 12%. Differentiating vaso-occlusive crises (VOC) from OM is a diagnostic challenge, with limited evidence guiding management. We present a 15-year review of a paediatric sickle cell cohort. We aim to identify OM incidence and provide a management protocol for these children presenting with bone pain. Methods A prospective database of children with haemoglobinopathies (2002 to 2017) was analyzed for temperature, C-reactive protein (CRP) and white cell count (WCC) on admission as well as imaging, treatment and cultures. OM diagnosis was supported by imaging and blood cultures. VOC was defined as bone pain that improved without antibiotics. Results Over 15 years, 96 children with SCD presented 358 times to hospital. Empirical antibiotics were given in 308 presentations. There were five cases of OM (1.4%); two acute and three chronic. In all, 50 presentations of VOC were identified. No significant differences in age were noted between the OM and VOC group. Temperature and CRP were significantly elevated in the OM group with no significant difference in WCC. Cultures were only positive in the chronic OM admissions. There were no cases of septic arthritis. No surgical intervention was required. Conclusion In children with SCD presenting with persistent bone pain, fever, elevated CRP and WCC, OM should be suspected and prompt antibiotic treatment started. Our treatment pathway was successful avoiding OM in 98.6% and septic arthritis in 100%. Further research on novel biological markers distinguishing OM from VOC should be investigated. Level of Evidence III
The effect of plasma generated ozone for cold storage the broiler chicken meat
Authors: MERISA YULIANI MERISA , MAHFUDZ L D , NURWANTORO NURWANTORO , NUR M 
Published: February 2019

Yuliani M, Mahfudz LD, Nurwantoro, Nur M. 2019. The effect of plasma generated ozone for cold storage the broiler chicken meat. Nusantara Bioscience 11: 12-17. The aim of this study was to determine the effect of combination treatment washing chicken meat with three ozone concentrations in the water during 10 min and stored at 2-7˚C without ozonation and with ozonation, on the shelf life of chicken meat. The study was conducted with a complete random factorial design (CRD) factorial pattern 3 x 2 with 3 replications. The data were analyzed by using analysis of variance (ANOVA). If the results were significant, the analysis followed Duncan's New Multiple Range Test (DMRT). The results of statistical analysis showed that average pH values, color scores intensity b * for brightness (yellowness) and Peroxide value (PV) showed significant differences (P <0.05). H 2 S test showed that combination treatment washing chicken meat with ozone concentrations 3ppm in the water during 10 min and stored at 2-7˚C with ozonation was able to inhibit the start process of decomposition chicken meat until the 10th days. The conclusion of these results that combination treatment washing chicken meat with ozone concentrations 3ppm in the water during 10 min and stored at 2-7˚C with ozonation can extend the shelf life of chicken meat until the 10th days.
The Possibilities of Different Geographies
Authors: Carr Jane , Sharp Bruce 
Published: February 2019

Abstract
The Possibilities of Different Geographies is the title of a dance work Jane Carr and Bruce Sharp first created in 1997 that investigated the significance of human embodiment. Twenty years later they revisited the themes informing their earlier work in order to create a participatory performance-installation focused on the significance of the embodied dimensions of inter-subjective experience.
The authors present the philosophical and political ideas underpinning their aims to challenge the boundaries that act as limits upon how humans experience their embodied identities and reflect on how, in developing the project, artistic and activist principles became interwoven. They describe the creation of movement scores for participants to perform and consider how elements of movement, sound, lighting and opportunities for reflection contribute to an environment that affords creative participation focused on the inter-corporeal dimension of human geographies.
Parenting and out-of-school music participation: An interrogation of concerted cultivation
Authors: Conkling Susan Wharton 
Published: February 2019

Abstract
I inquired with parents and primary caregivers about why they supported their children’s participation in music activities in an out-of-school space and what they expected from such participation. To interrogate Lareau’s concept of concerted cultivation, I was interested in exploring the extent to which there might be differences in parental or primary caregiver expectations by race or socio-economic status. In the Boston Children’s Chorus (BCC), I found a community youth music programme where household income, race and ethnicity were reasonably diversified. Structured interviews with 132 parents and primary caregivers suggested that practices and discourse most worth analysis in regard to parenting and community music operate not at the level of race, ethnicity or social class, as Lareau suggests, but instead they operate at a local network level. The ways in which parent or caregiver trust in the community youth music programme developed through these local networks, and the ways in which the community youth music programme may have expanded social networks for families emerged as themes for further examination.
The role of community-based music and sports programmes in parental views of children’s social skills and personality
Authors: Ilari Beatriz , Perez Priscilla , Wood Alison , Habibi Assal 
Published: February 2019

Abstract
In this article, we report on parental perceptions of socio-emotional skills and personality of children who were involved in community-based music and sports extracurricular (EC) programmes, and a group of children not participating in EC activities. This study is part of the USC brain and music project that investigated the effects of music education on the cognitive and socioemotional development of children from underserved neighbourhoods in Los Angeles for 5 consecutive years. Our primary aim with this study was to examine parental views on the potential effects of EC programmes on children’s socio-emotional skills and personality. Parents were interviewed yearly and were asked to rate their child’s skills and personality. After four years, parents of children involved in EC activities rated their children higher on the emotional stability personality trait, lower on aggression and lower on hyperactivity compared to children not involved in EC activities despite no differences in these measures at the beginning of the study and before children’s entry into their programmes. These and other findings are discussed in light of Bronfenbrenner and Morris’s Bioecological Model of Human Development. The implications for community music are also outlined.
Menopausal Hormone Therapy and Risk of Diverticulitis
Authors: Jovani Manol , Ma Wenjie , Joshi Amit D. , Liu Po-Hong , Nguyen Long H. , Cao Yin , Tam Idy , Wu Kana , Giovannucci Edward L. , Chan Andrew T. , Strate Lisa L. 
Published: February 2019

Introduction:
Diverticulitis is a significant cause of morbidity among older women, and little attention has been paid to understanding its etiology. We have shown that menopausal hormone therapy (MHT) is associated with the risk of inflammatory bowel disease. In this study, we prospectively examined the association between MHT and the risk of incident diverticulitis.

Methods:
We studied 65,367 postmenopausal women enrolled in the Nurses' Health Study who provided detailed information on hormone use and other medical and lifestyle factors biennially, and on diet every 4 years. Between 2008 and 2014, participants reported any episodes of diverticulitis that required antibiotics and the date of occurrence. We used Cox proportional hazards regression models to estimate multivariable-adjusted hazard ratios (HRs) and 95% confidence intervals (CIs).

Results:
Over 24 years encompassing 1,297,165 person-years of follow-up, we documented 5,425 incident cases of diverticulitis. We observed an increased risk of diverticulitis among both current (HR 1.28; 95% CI 1.18–1.39) and past (HR 1.35; 95% CI 1.25–1.45) MHT users compared to never users. The increased risk was observed among participants using estrogen only (HR 1.30; 95% CI 1.20–1.41) and those using combined estrogen and progesterone (HR 1.31; 95% CI 1.21–1.42) compared to nonusers. The risk did not increase with longer duration of use (P-trend = 0.76). The association between MHT and diverticulitis was not modified by age, body mass index, past oral contraceptive use, or fiber intake (all P-interaction >0.11).

Conclusions:
Menopausal hormone therapy was associated with an increased risk of diverticulitis. Further studies are needed to understand the potential mechanisms that may underlie this association.
À propos du « monde ancien » dans l’œuvre d’Henry Bauchau : une approche du Boulevard périphérique
Authors: HALEN Pierre 
Published: February 2019

Pierre Halen propose dans cet article une approche du roman Le Boulevard périphérique d’Henry Bauchau. C’est un roman en partie autobiographique qui brasse des questions difficiles, inaudibles peut-être jusqu’à un certain point, qui en font une sorte de méditation sur le XXe siècle que l’auteur a traversé de part en part. Plus explicitement que jamais dans l’œuvre de Bauchau, le sujet qui s’énonce en « je » dans Le Bouvevard périphérique est en effet le témoin à la fois attentif et circonspect d’une histoire collective, d’un « nous ». Or, cette histoire s’énonce difficilement, charriant ces moments lumineux que le sujet ne peut que célébrer, mais aussi ce qu’il s’angoisse d’avoir peut-être perdu.
Henry Bauchau : de la nécessité dans la création
Authors: POUTIER Irène 
Published: February 2019

Irène Poutier propose dans cet article un rapprochement avec Antonin Artaud et Simone Weil, deux compagnons plus ou moins masqués de l’œuvre de Bauchau, pour mieux souligner l’impérieuse nécessité que représente l’art pour cet écrivain : celle de se soumettre à une forme d’obéissance pour créer, d’accepter sa condition pour y parvenir, et de reconnaître dans ses infirmités – la cécité notamment – la possibilité même de la vision et de la créativité.
L’ art comme lieu du sens et de la vérité corporelle chez Henry Bauchau
Authors: LECLERCQ Jean 
Published: February 2019

Jean Leclercq propose une étude du journal d’Henry Bauchau Le Présent d’incertitude, selon la double perspective d’un questionnement de l’écrivain de sa propre pratique artistique comme de celle de ses personnages, Orion en l’occurrence : art en acte, roman de l’art en acte… Dans les deux cas, il semble que l’expérience artistique élabore dans une symbiose constructive ou reconstructrice un naturalisme spirituel, une « mystique océane », faisant de la création une expression d’expériences pures, un moment de reviviscence heureuse des affects de la vie.
Le récit mythique dans Géologie : l’émergence d’une parole-trace.
Authors: LAMBERT Jérémy 
Published: February 2019

L’article se penche sur l’ensemble intitulé « Caste des Guerriers » inclus dans Géologie, premier recueil poétique publié par Henry Bauchau. Il y relève, dans une lecture minutieuse du texte, les traces de la mythologie nordique et montre l’utilisation qui en est faite par le poète pour exprimer sa recherche de sens propre. Il souligne l’exploitation d’une « matière-émotion », la langue poétique devenant indice, trace d’un au-delà du signe.
Naissance des Antigones
Authors: HENROT Geneviève 
Published: February 2019

L’article propose une étude génétique du poème « Les deux Antigone », rédigé d’août à octobre 1982. L’étude matérielle et génétique du dossier témoigne d’une réécriture par vagues. Par le biais d’une étude syntaxique, sémantico-référentielle et actancielle centrée sur les reprises, on voit apparaître un faisceau de traits communs entre « L’escalier bleu » et « Les deux Antigone ». Mais surtout, on observe le traitement du personnage d’Antigone qui devient une qualité d’être à laquelle l’identification affective et spirituelle devient possible pour le poète.
Endosymbiont bacteria Holospora undulata confers oxidative tolerance in host Paramecium caudatum
Authors: Stewart Rainey A 
Published: February 2019

Paramecium is a genus of ciliated protozoan that, while unicellular, has a complex intracellular structure, comparable to Metazoan cells, which has made them excellent models for the study of genetics and cellular functions. Holospora undulata is a bacterial endosymbiont specific to the species Paramecium caudatum; they are unable to grow outside of P. caudatum. The presence of this endosymbiont has proven to have an effect on the subsequent gene expression and cellular maintenance of its host cells. Recent studies have demonstrated that infection by H. obtusa increases the expression of host heat-shock genes and leads to both resistance at normally-lethal high temperatures and heat resistance in ciliary movement (Fujishima, Kawai, & Yamamoto, 2005; Hori & Fujishima, 2003). Heat-shock resistance occurs because bacterial DNA triggers the upregulation of its P. caudatum host’s heat-shock genes (i.e., hsp60 and hsp70), although the mechanisms are not known (Hori & Fujishima, 2003). These studies demonstrate that infection of P. caudatum by H. undulata (a closely-related species to H. obtusa) induces heat-shock resistance, but fail to address whether H. undulata protects against other common environmental stressors such as oxidative damage. To determine if infection by H. undulata has the ability to induce additional tolerances, we examined differences in oxidative tolerance, based on percent survival, between P. caudatum with and without H. undulata infection. Samples of both lines were treated with increasing concentrations of hydrogen peroxide, the number of surviving cells were counted, and the percent survivability of each sample was calculated. There was an approximate 20% increase in survival when P. caudautum was infected with H. undulata—thus H. undulata infections confer oxidative tolerance. Further studies will be conducted to determine if an increase in survivability occurs in response to other damaging mechanisms. Future work will also investigate if the genes responsible for oxidative damage repair are upregulated, in addition to the already characterized heat-shock genes.