bluuebunny/crossref_metadata_2025_split huggingface dataset:
A comparison of spatial heterogeneity with local cluster detection methods for chronic respiratory diseases in Thailand
Authors: Laohasiriwong Wongsa , Puttanapong Nattapong , Luenam Amornrat 
Published: March 2018

Background: The Centers for Disease Control and Prevention reported that deaths from chronic respiratory diseases (CRDs) in Thailand increased by almost 13% in 2010, along with an increased burden related to the disease. Evaluating the geographical heterogeneity of CRDs is important for surveillance. Previous studies have indicated that socioeconomic status has an effect on disease, and that this can be measured with variables such as night-time lights (NTLs) and industrial density (ID). However, there is no understanding of how NTLs and ID correlate with CRDs. We compared spatial heterogeneity obtained by using local cluster detection methods for CRDs and by correlating NTLs and ID with CRDs. Methods: We applied the spatial scan statistic in SaTScan, as well as local indices of spatial association (LISA), Getis and Ord’s local Gi*(d) statistic, and Pearson correlation. In our analysis, data were collected on gender, age, household income, education, family size, occupation, region, residential area, housing construction materials, cooking fuels, smoking status and previously diagnosed CRDs by a physician from the National Socioeconomic Survey, which is a cross-sectional study conducted by the National Statistical Office of Thailand in 2010. Results: According to our findings, the spatial scan statistic, LISA, and the local Gi*(d) statistic revealed similar results for areas with the highest clustering of CRDs. However, the hotspots for the spatial scan statistic covered a wider area than LISA and the local Gi*(d) statistic. In addition, there were persistent hotspots in Bangkok and the perimeter provinces. NTLs and ID have a positive correlation with CRDs. Conclusions: This study demonstrates that all the statistical methods used could detect spatial heterogeneity of CRDs. NTLs and ID can serve as new parameters for determining disease hotspots by representing the population and industrial boom that typically contributes to epidemics.
The effect of a health literacy approach to counselling on the lifestyle of women with gestational diabetes: A clinical trial
Authors: Gharachourlo Mehrafza , Mahmoodi Zohreh , Akbari Kamrani Mahnaz , Tehranizadeh Maryam , Kabir Kourosh 
Published: March 2018

Background: Gestational diabetes is a common pregnancy disorder that affects the mother’s and neonate’s health. The present study was conducted to investigate the effect of a health literacy approach to counselling on the lifestyle of women with gestational diabetes. The present randomized controlled clinical trial was conducted in 2017 using a parallel design. The subjects included 84 eligible women presenting to Alborz and Kamali Hospitals, Karaj, Iran. Methods: Convenience sampling was first used to select the subjects. They were then assigned to an intervention or control group based on randomized blocks of four. Both groups attended counselling sessions. The mothers in the intervention group attended six sessions of counselling with a health literacy approach in addition to counselling on routine pregnancy care. The control group attended counselling sessions on safe pregnancy care and received a training package containing all the subjects discussed in the intervention group. The Lifestyle Questionnaire and the Iranian Health Literacy Questionnaire were completed by the mothers at the beginning and at the end of the sessions as well as three weeks after the sessions. The data obtained were analyzed in SPSS-19. Results: According to the study findings, the scores of lifestyle (P=0.8) and health literacy (P=0.423) showed no significant differences between the intervention and control groups before the intervention. Significant differences were, however, observed in the mean scores of lifestyle and health literacy between the two groups immediately and three weeks after the intervention. Comparing the means showed a higher increase in the mean scores in the intervention group (P<0.001). Conclusions: Providing counselling services by midwives can significantly help modify mothers’ unhealthy lifestyle choices and increase their health literacy; therefore, reducing maternal and neonatal consequences, especially in high-risk pregnancies. Trial registration number: IRCT2017021427728N3 Trial registry: Iranian Registry of Clinical Trials Trial registration date: 5th April 2017
Investigating the role of Rts1 in DNA replication initiation
Authors: Wallis Ana B.A. , Nieduszynski Conrad A. 
Published: March 2018

Background:Understanding DNA replication initiation is essential to understand the mis-regulation of replication seen in cancer and other human disorders. DNA replication initiates from DNA replication origins. In eukaryotes, replication is dependent on cell cycle kinases which function during S phase. Dbf4-dependent kinase (DDK) and cyclin-dependent kinase (CDK) act to phosphorylate the DNA helicase (composed of mini chromosome maintenance proteins: Mcm2-7) and firing factors to activate replication origins. It has recently been found that Rif1 can oppose DDK phosphorylation. Rif1 can recruit protein phosphatase 1 (PP1) to dephosphorylate MCM and restricts origin firing. In this study, we investigate a potential role for another phosphatase, protein phosphatase 2A (PP2A), in regulating DNA replication initiation. The PP2A regulatory subunit Rts1 was previously identified in a large-scale genomic screen to have a genetic interaction withORC2(a DNA replication licensing factor). Deletion ofRTS1synthetically rescued the temperature-sensitive (ts-) phenotype ofORC2mutants.Methods:We deletedRTS1in multiple ts-replication factorSaccharomyces cerevisiaestrains, includingORC2. Dilution series assays were carried out to compare qualitatively the growth of double mutant∆rts1ts-replication factor strains relative to the respective single mutant strains. Results:No synthetic rescue of temperature-sensitivity was observed. Instead we found an additive phenotype, indicating gene products function in separate biological processes. These findings are in agreement with a recent genomic screen which found thatRTS1deletion in several ts-replication factor strains led to increased temperature-sensitivity.Conclusions:We find no evidence that Rts1 is involved in the dephosphorylation of DNA replication initiation factors.
Community engagement for malaria elimination in contested areas of the Karen/Kayin State, Myanmar: A case study on the Malaria Elimination Task Force
Authors: König Andrea , Kajeechiwa Ladda , Thwin May Myo , Nosten Suphak , Tun Saw Win , Tangseefa Decha , Nosten François 
Published: March 2018

Background: This study evinces how the community engagement (CE) approach of the Malaria Elimination Task Force (METF) in the Karen/Kayin State of southeast Myanmar contributed to generating participation in program activities, such as early detection and treatment, malaria blood surveys and mass drug administration. Methods: We investigated the CE approach through ethnographic and key-informant unstructured interviews as well as focus group discussions and participant observation with program participants METF team members. Interview transcripts were analysed manually applying inductive content analysis. Results: In the context of the Thai-Myanmar border, CE had two main dimensions: The first was to enable the politically sensitive entry of the program into intervention areas, the second was to create mutual understanding among the METF team and people from the population and gain trust. For the first, the METF needed to acknowledge, get permission and communicate with a complex landscape of governing actors and leaders. For the second, creating mutual understanding meant to spend time learning about villagers’ lifeworlds and adapting engagement accordingly to create understanding of the program in resonance with these lifeworlds. Gaining trust meant to leverage leadership and build trust through informal “horizontal” engagement and acts of caring. Three key dimensions of villagers’ lifeworlds that appeared most crucial for staff to learn about were: villagers’ experience with the outside world; leadership and governing relationships to villagers: authority and distrust; and their perceived need for malaria care. Conclusions: Successful CE is highly context specific. CE success was based on the adaptability of the approach towards the complex political and socio-cultural contexts of the different areas of the Karen/Kayin State. Hence, CE is not a fixed approach or strategy defined before entering the setting, but constantly evolving based on the encounters with stakeholders and people of the population.
Convergent morphology in Alpinieae (Zingiberaceae): Recircumscribing Amomum as a monophyletic genus
Authors: Boer Hugo de , Newman Mark , Poulsen Axel Dalberg , Droop A. Jane , Fér Tomáš , Thu Hiền Lê Thi , Hlavatá Kristýna , Lamxay Vichith , Richardson James E. , Steffen Karin , Leong-Škorničková Jana 
Published: March 2018

AbstractThe tropical ginger genus Amomum (Zingiberaceae) has always posed challenges for classification based on morphological characters. Previous molecular phylogenetic studies showed Amomum to be paraphyletic but limited sampling and absence of the data of the type Amomum subulatum made it impossible to resolve the paraphyly and make nomenclatural changes. Here, Amomum is further investigated in a multi–marker phylogenetic framework using matK and nrITS including multiple accessions of the type, the genus Elettaria and additional accessions of Amomum, Alpinia, Elettariopsis, Geocharis, Geostachys and Hornstedtia. Amomum is shown to consist of nine clades and Alpinia of six. The genera Elettaria, Elettariopsis, Plagiostachys, and species in Hornstedtia are nested within these clades. Morphological studies of species previously subsumed in Amomum support recognition of new genera that correspond to well–delimited clades in the phylogenetic framework presented here. Recircumscription of the paraphyletic genus Amomum facilitates identification and creates nomenclatural stability. Three genera, Conamomum, Meistera and Wurfbainia, are resurrected, and three new genera Epiamomum, Lanxangia and Sundamomum are described, together with a key to the genera and a nomenclatural synopsis placing 384 specific names (incl. all synonyms) into the new generic framework. Of these 129 represent new combinations and 3 are replacement names. Types of Geocharis and Geostachys are designated. Further studies and specific sampling will be needed to resolve other branches of Alpinioideae containing other polyphyletic genera.
Lachesiodendron, a new monospecific genus segregated from Piptadenia (Leguminosae: Caesalpinioideae: mimosoid clade): Evidence from morphology and molecules
Authors: Ribeiro Pétala Gomes , Luckow Melissa , Lewis Gwilym P. , Simon Marcelo F. , Cardoso Domingos , de Souza Élvia R. , Conceição Silva Ana Paula , Jesus Marcel Carvalho , dos Santos Francisco A.R. , Azevedo Vânia , de Queiroz Luciano Paganucci 
Published: March 2018

AbstractThe widest historical circumscription of the mimosoid legume genus Piptadenia (Caesalpinioideae: mimosoid clade) previously embraced morphologically disparate species that are currently ascribed to nine different genera. Previous molecular phylogenetic studies have demonstrated that the neotropical dry forest Piptadenia viridiflora is not closely related to Piptadenia s.str. Here we plug gaps in the DNA sequence dataset of a recently published study to build a more comprehensive phylogeny of the Piptadenia group and revise morphological evidence to define the phylogenetic relationship of P. viridiflora. Both morphological and molecular data confirm that P. viridiflora is an isolated lineage, thus warranting the description of the new monospecific genus Lachesiodendron. The new genus is diagnosed by the combination of paired stipular spines (vs. stipules not modified into spines in Piptadenia s.str.), prickles absent (vs. present), flowers in axillary spikes not grouped in compound inflorescences (vs. spikes grouped in compound pseudoracemes or panicles), flowers with a cylindrical corolla long exserted from the calyx (vs. corolla campanulate with petals free or slightly joined at the base) and long green stamens (vs. shorter and white, pale yellow or pink stamens). In addition, Lachesiodendron has pollen aggregated into 8–celled polyads with a hamulate exine, which further contrasts with the 12–celled polyads with a psilate exine in Piptadenia s.str.
Functioning of the various forms of mental health care in Poland in the years 2010–2013. Organizational, economic and financial aspects
Authors: Lisiecka-Biełanowicz Mira , Biechowska Daria , Cianciara Dorota 
Published: March 2018

Cel pracyGłównym celem analizy była weryfikacja hipotezy o znaczącym wzroście nakładów na świadczenia psychiatryczne na przestrzeni ostatnich lat. Skutkiem wysokiej dynamiki przyrostu nakładów będzie rozwój modelu psychiatrii środowiskowej oraz stopniowe odejście od szpitalnej opieki psychiatrycznej na rzecz opieki w systemie otwartym, w tym środowiskowym.MetodaW badaniu wykorzystano informacje o realizacji świadczeń w zakresie psychiatrycznej opieki zdrowotnej w ramach umów zawartych z NFZ, które zostały zgromadzone w systemie informatycznym Centrali NFZ. Część informacji pochodzi z roku 2010, który przyjęto jako datę bazową dla rozpoczęcia realizacji założeń Narodowego Programu Ochrony Zdrowia Psychicznego w 2011 roku. W celu porównania wykorzystano dane z realizacji poszczególnych świadczeń w roku 2013. Ponadto analizowano inne wybrane elementy organizacyjne i ekonomiczno-finansowe systemu opieki psychiatrycznej.WynikiW 2013 r. w stosunku do roku 2010 wzrosła liczba podmiotów psychiatrycznej opieki zdrowotnej: poradni zdrowia psychicznego (wzrost o 37 poradni), psychiatrycznych oddziałów dziennych (25 oddziałów) oraz zespołów leczenia środowiskowego (74 zespoły). Największy wzrost wartości kontraktów (ok. 150%) dotyczył zespołów leczenia środowiskowego.WnioskiMiędzy rokiem 2010 a 2013 wrosła wartość rozliczonych kontraktów w opiece psychiatrycznej, ogólnie i w każdej z trzech form opieki psychiatrycznej (tj. na oddziale dziennym, w poradniach zdrowia psychicznego oraz w zespołach środowiskowych). Największy wzrost nakładów objął zespoły leczenia środowiskowego, w mniejszym stopniu oddziały dzienne i ambulatoria. Przyjęte rozwiązania organizacyjne i ekonomiczno-finansowe w systemie opieki psychiatrycznej są zgodne z celami Narodowego Programy Ochrony Zdrowia Psychicznego, w tym zakładaną strukturą Centrów Zdrowia Psychicznego.
Are suicide risk factors gender specific?
Authors: Pawlak Joanna , Miechowicz Izabela , Dmitrzak-Węglarz Monika , Szczepankiewicz Aleksandra , Zaremba Dorota , Kapelski Paweł , Rajewska-Rager Aleksandra , Czerski Piotr , Skibińska Maria , Hauser Joanna 
Published: March 2018

Cel pracySamobójstwo stanowi istotne zagadnienie w opiece nad pacjentami psychiatrycznymi. Najwyższe ryzyko obserwowane jest u osób z zaburzeniami afektywnymi. Na podstawie literatury wytypowano 20 czynników związanych z zachowaniami samobójczymi (czynniki socjo-demograficzne, kliniczne i obciążenia rodzinne) i przeanalizowano ich znaczenie zależnie od płci pacjentów.MetodaDo badania włączono chorych z rozpoznaniem dużej depresji (MDD; n=249) i zaburzeń depresyjnych dwubiegunowych (BP; n=582). Zastosowano kwestionariusze SCID I (Structured Clinical Interview for DSM-IV Axis I), OPCRIT (Operational Criteria Diagnostic Checklist) oraz kwestionariusz obciążeń rodzinnych.WynikiW badanej populacji stwierdzono związek prób samobójczych z następującymi czynnikami: obciążenie rodzinne zaburzeniami psychicznymi, zaburzeniami afektywnymi i nadużywaniem/uzależnieniem od substancji psychoaktywnych, obciążenie rodzinne próbami/dokonanymi samobójstwami, występowanie w przebiegu epizodów depresyjnych następujących objawów: nadmierne poczucie winy, poczucie bezwartościowości i przedwczesne budzenie się rano oraz z objawami psychotycznymi. Wykazano także związek prób samobójczych w wywiadzie z posiadaniem potomstwa. Czynniki związane z próbami samobójczymi różniły się w podgrupach kobiet i mężczyzn. Wiek początku MDD i współwystępowanie z zaburzeniami afektywnymi nadużywania/uzależnienia od substancji psychoaktywnych było związane z próbami samobójczymi wyłącznie w grupie kobiet. Posiadanie potomstwa było związane z próbami samobójczymi w całej grupie oraz w podgrupie mężczyzn, natomiast nie miało znaczenia w podgrupie kobiet.WnioskiStwierdzono związek prób samobójczych z dziesięcioma spośród wytypowanych 20 czynników, jednak ich istotność (lub jej brak) różniła się pomiędzy grupą pacjentów płci męskiej i płci żeńskiej aż w połowie przypadków.
The Japanese registry for surgery of ischial pressure ulcers: STANDARDS-I
Authors: Yanagi Hideyuki , Terashi Hiroto , Takahashi Yoshimitsu , Okabe Katsuyuki , Tanaka Katsumi , Kimura Chu , Ohura Norihiko , Goto Takahiro , Hashimoto Ichiro , Noguchi Madoka , Sasayama Junichi , Shimada Kenichi , Sugai Ayumi , Tanba Mitsuko , Nakayama Takeo , Tsuboi Ryoji , Sugama Junko , Sanada Hiromi 
Published: March 2018

Objective: To clarify the surgical indications and the appropriate perioperative management of ischial pressure ulcers (PUs). Method: A two-year prospective, nationwide registry study was carried out across 26 medical institutions in Japan. All participating institutions managed ischial PUs according to the standardisation of total management and surgical application for the refractory decubitus (STANDARDS-I) perioperative protocol. Analysis was conducted on a range of clinically or statistically important variables for the achievement of primary or secondary endpoints: complete wound healing and hospital discharge at three months, and complete wound healing at one month after surgery, respectively. Results: A total of 59 patients took part in the study. All patients underwent surgery for ischial PUs during the study period. Patients who had achieved the primary endpoint had a higer preoperative functional independence measurement (FIM score), a higher ‘G’ score in the DESIGN-R scale and were more likely to have healed by primary intention. Patients who had achieved the secondary endpoint were more likely to have spastic paralysis, preoperative physiotherapy and localised infection of the wound, among other variables. Conclusion: This survey suggests that preoperative physiotherapy increases the speed of wound healing, and good granulation of the wound bed preoperatively increases the likelihood of woundless discharge from hospital, whereas the existence of comorbidities negatively influences the likelihood of woundless discharge from hospital. The study also suggests that the existence of spastic paralysis, preoperative infection of the wound, or surgical reduction of the ischial tubercle speeds up the healing of the wound. However, the wound failed to heal significantly more often in patients with increasing white blood cell count after surgery.
Urtica dioica inhibits cell growth and induces apoptosis by targeting Ornithine decarboxylase and Adenosine deaminase as key regulatory enzymes in adenosine and polyamines homeostasis in human breast cancer cell lines
Authors: Fattahi Sadegh , Ghadami Elham , Asouri Mohsen , Motevalizadeh Ardekanid Ali , Akhavan-Niaki Haleh 
Published: March 2018

Breast cancer is a heterogeneous and multifactorial disease with variable disease progression risk, and treatment response. Urtica dioica is a traditional herb used as an adjuvant therapeutic agent in cancer. In the present study, we have evaluated the effects of the aqueous extract of Urtica dioica on Adenosine deaminase (ADA) and Ornithine decarboxylase (ODC1) gene expression in MCF-7, MDA-MB-231, two breast cancer cell lines being estrogen receptor positive and estrogen receptor negative, respectively. Cell lines were cultured in suitable media. After 24 h, different concentrations of the extract were added and after 72 h, ADA and ODC1 gene expression as well as BCL2 and BAX apoptotic genes were assessed by Taqman real time PCR assay. Cells viability was assessed by MTT assay, and apoptosis was also evaluated at cellular level. The intra and extracellular levels of ODC1 and ADA enzymes were evaluated by ELISA. Results showed differential expression of ADA and ODC1 genes in cancer cell lines. In MCF-7 cell line, the expression level of ADA was upregulated in a dose-dependent manner but its expression did not change in MDA-MB cell line. ODC1 expression was increased in both examined cell lines. Also, increased level of the apoptotic BAX/BCL-2 ratio was detected in MCF-7 cells. These results demonstrated that Urtica dioica induces apoptosis in breast cancer cells by influencing ODC1 and ADA genes expression, and estrogen receptors. The different responses observed with these cell lines could be due to the interaction of Urtica dioica as a phytoestrogen with the estrogen receptor.
A rare case of complicated pericardial effusion with Elizabethkingia meningoseptica from Iran
Authors: Sedigh Ebrahim-Saraie Hadi , Heidari Hamid , Khashei Reza , Nabavizadeh Seyed Hesamedin 
Published: March 2018

Infections due to Elizabethkingia meningoseptica, a Gram-negative oxidative bacterium are frequently founded in neonatal and immunocompromised individuals. The notable characteristic of this organism is its multi-drug resistance to common antibiotics used for infections caused by Gram-negative bacteria. We report a rare case of complicated pericardial effusion due to E. meningoseptica in a 2-year-old boy, who was admitted with chief complaints of fever and tachypnea (mentioned by his parents) and suffered from a rare lung malignancy (lymphangioleiomyomatosis). He was successfully treated with vancomycin. E. meningoseptica infection is a rare situation in immunocompetent hosts, and we concluded that this infection was probably originated from device medicine or even hands of healthcare workers.
A placebohatás és az alkohol
Authors: Bodnár Vivien , Nagy Krisztina , Cziboly Ádám , Bárdos György 
Published: March 2018

Elméleti háttér
A placebohatás a mindennapi élet számos területén tetten érhető, amikor valamely pszichoaktív szerhez kötődő elvárás vezet szubjektív és fiziológiai változásokhoz, hatóanyag jelenléte nélkül.

Cél:
Szeretnénk feltárni, hogy az alkohol és a hozzá kapcsolódó elvárások – a társas hatásokkal együtt, illetve azoktól elkülönítve – hogyan befolyásolják a résztvevőket, különösen a memória, az egyensúly, és a szubjektív testi-lelki állapot kapcsán, az alkoholos, alkoholosnak vélt, illetve alkoholt nem tartalmazónak gondolt koktélok elfogyasztása esetében.

Módszerek
Kiegyenlített placebo elrendezésben, társas és egyéni feltételekben vizsgáltuk a rövidtávú memória, az egyensúlyérzék, és a szubjektív testi-lelki állapot alakulását, szó lista visszaidézése, „részegállás” megtartása, és egy 17 melléknévpárból álló szemantikus differenciál skála felhasználásával.

Eredmények
A szubjektív testi-lelki állapot mérése során feltárt szociális viselkedések és testi tünetek faktor mentén a vizsgált csoportok eltérő mintázatot mutattak: a résztvevők a manipulációnak megfelelő elvárásaik mentén számoltak be szubjektív tüneteikről, amelyeket a csoporthatások tovább erősítettek. A vizsgálatban mind egyéni, mind pedig társas fogyasztás esetében sikerült kimutatni az elvárások által indukált klasszikus placebohatást a megtévesztett, alkoholt valójában nem fogyasztók körében. Az alkoholt és a placebót fogyasztó csoport sem a szociális viselkedések, sem a testi tünetek, sem pedig az egyensúly romlása mentén nem különbözik szignifikánsan egymástól. A csoportos alkoholfogyasztás tovább erősíti az alkohol valós vagy vélt hatásait az egyensúlyra és a memóriára.

Következtetések
Kis mennyiségű alkohol fogyasztása esetében az alkohol indukálta állapot kialakulásáért az etanol csak részben felelős, az alkoholfogyasztást követő pszichés és fizikai változások jelentős része – elvárások és a társas közeg által meghatározottan – a placebohatásra vezethető vissza.