bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hippo Signaling Pathway Dysregulation in Human Huntington’s Disease Brain and Neuronal Stem Cells
Authors: Mueller Kaly A. , Glajch Kelly E. , Huizenga Megan N. , Wilson Remi A. , Granucci Eric J. , Dios Amanda M. , Tousley Adelaide R. , Iuliano Maria , Weisman Elizabeth , LaQuaglia Michael J. , DiFiglia Marian , Kegel-Gleason Kimberly , Vakili Khashayar , Sadri-Vakili Ghazaleh 
Published: July 2018

AbstractThe Hippo signaling pathway is involved in organ size regulation and tumor suppression. Although inhibition of Hippo leads to tumorigenesis, activation of Hippo may play a role in neurodegeneration. Specifically, activation of the upstream regulator, mammalian sterile 20 (STE20)-like kinase 1 (MST1), reduces activity of the transcriptional co-activator Yes-Associated Protein (YAP), thereby mediating oxidative stress-induced neuronal death. Here, we investigated the possible role of this pathway in Huntington’s disease (HD) pathogenesis. Our results demonstrate a significant increase in phosphorylated MST1, the active form, in post-mortem HD cortex and in the brains of CAG knock-in HdhQ111/Q111 mice. YAP nuclear localization was also decreased in HD post-mortem cortex and in neuronal stem cells derived from HD patients. Moreover, there was a significant increase in phosphorylated YAP, the inactive form, in HD post-mortem cortex and in HdhQ111/Q111 brain. In addition, YAP was found to interact with huntingtin (Htt) and the chaperone 14-3-3, however this interaction was not altered in the presence of mutant Htt. Lastly, YAP/TEAD interactions and expression of Hippo pathway genes were altered in HD. Together, these results demonstrate that activation of MST1 together with a decrease in nuclear YAP could significantly contribute to transcriptional dysregulation in HD.
Toward Electrically Tunable, Lithography-Free, Ultra-Thin Color Filters Covering the Whole Visible Spectrum
Authors: Aalizadeh Majid , Serebryannikov Andriy E. , Khavasi Amin , Vandenbosch Guy A. E. , Ozbay Ekmel 
Published: July 2018

AbstractThe possibility of real-time tuning of optical devices has attracted a lot of interest over the last decade. At the same time, coming up with simple lithography-free structures has always been a challenge in the design of large-area compatible devices. In this work, we present the concept and the sample design of an electrically tunable, lithography-free, ultra-thin transmission-mode color filter, the spectrum of which continuously covers the whole visible region. A simple Metal-Insulator-Metal (MIM) cavity configuration is used. It is shown that using the electro-optic dielectric material of 4-dimethyl-amino-N-methyl-4-stilbazoliumtosylate (DAST) as the dielectric layer in this configuration enables efficient electrical tuning of the color filter. The total thickness of the structure is 120 nm, so it is ultra-thin. The output color gets tuned from violet to red by sweeping the applied voltage from −12 to +12 Volts (V). We present an in-detail optimization procedure along with a simple calculation method for the resonance wavelength of the MIM cavity that is based on circuit theory. Such power-efficient structures have a large variety of potential applications ranging from optical communication and switching to displays and color-tunable windows.
Hordes of Phages in the Gut of the Tilapia Sarotherodon melanotheron
Authors: Bettarel Yvan , Combe Marine , Adingra Antoinette , Ndiaye Awa , Bouvier Thierry , Panfili Jacques , Durand Jean-Dominique 
Published: July 2018

AbstractPreliminary studies conducted on the human gastro-intestinal tract have revealed that enteric viral communities play a preponderant role in microbial homeostatis. However to date, such communities have never been investigated in the fish gut. Herein, we examined the main ecological traits of viruses in the digestive tract of a euryhaline fish, the tilapia Sarotherodon melanotheron. Individuals were collected at 8 different sites in Senegal covering a salinity gradient from 3 to 104‰, and showing large disparities in their organic pollutant concentrations. Results showed that the gut of S. melanotheron is home to a highly abundant viral community (0.2–10.7 × 109 viruses ml−1), distinct from the surrounding water, and essentially composed of phages of which a substantial proportion is temperate (the fraction of lysogenized cells-FLC ranging from 8.1 to 33.0%). Also, a positive and significant correlation was detected between FLC and the concentrations of polycyclic aromatic hydrocarbon in sediment, while no clear relationships were found between salinity and any of the microbial parameters considered. Finally, our data suggest that virus-bacteria interactions within the fish intestine are likely sensitive to the presence of particular xenobiotics, which may compromise the balance in the gut microbiota, and subsequently affect the health of their host.
Increased aqueous autotaxin and lysophosphatidic acid levels are potential prognostic factors after trabeculectomy in different types of glaucoma
Authors: Igarashi Nozomi , Honjo Megumi , Kurano Makoto , Yatomi Yutaka , Igarashi Koji , Kano Kuniyuki , Aoki Junken , Aihara Makoto 
Published: July 2018

AbstractWe explored the potential relevance of aqueous lysophosphatidic acid (LPA) and autotaxin (ATX) levels on postoperative outcomes of trabeculectomy, and the effects of ATX on fibrotic response in cultured human conjunctiva fibroblast (HCF) cells. We enrolled 70 glaucomatous eyes which underwent trabeculectomy, and quantified aqueous LPA and ATX. Those eyes were followed up for 12 months, and postoperative filtering blebs were evaluated using anterior segment optical coherence tomography. Also, the ATX-induced fibrotic changes in HCFs and the effects of an ATX inhibitor were assessed. Measured aqueous ATX and LPA levels were significantly different between glaucoma subtypes. In multivariate analyses, aqueous ATX levels were significantly correlated with the presence of needlings at 1, 3, 6 and 12 months after surgery. Exfoliative glaucoma, whose ATX level was significantly high, showed significantly increased numbers of needlings and a lower cumulative success rate without needlings. An in vitro study showed that fibrotic changes were upregulated by ATX treatment in HCFs, which was significantly suppressed by an ATX inhibitor. We presently demonstrate that aqueous ATX may be a prognostic factor affecting the fibrotic response in HCFs and bleb formation, and inhibition of ATX could be a therapeutic target after trabeculectomy.
Exploring the pathology of an epidermal disease affecting a circum-Antarctic sea star
Authors: Núñez-Pons Laura , Work Thierry M. , Angulo-Preckler Carlos , Moles Juan , Avila Conxita 
Published: July 2018

AbstractOver the past decade, unusual mortality outbreaks have decimated echinoderm populations over broad geographic regions, raising awareness globally of the importance of investigating such events. Echinoderms are key components of marine benthos for top-down and bottom-up regulations of plants and animals; population declines of these individuals can have significant ecosystem-wide effects. Here we describe the first case study of an outbreak affecting Antarctic echinoderms and consisting of an ulcerative epidermal disease affecting ~10% of the population of the keystone asteroid predator Odontaster validus at Deception Island, Antarctica. This event was first detected in the Austral summer 2012–2013, coinciding with unprecedented high seawater temperatures and increased seismicity. Histological analyses revealed epidermal ulceration, inflammation, and necrosis in diseased animals. Bacterial and fungal alpha diversity was consistently lower and of different composition in lesioned versus unaffected tissues (32.87% and 16.94% shared bacterial and fungal operational taxonomic units OTUs respectively). The microbiome of healthy stars was more consistent across individuals than in diseased specimens suggesting microbial dysbiosis, especially in the lesion fronts. Because these microbes were not associated with tissue damage at the microscopic level, their contribution to the development of epidermal lesions remains unclear. Our study reveals that disease events are reaching echinoderms as far as the polar regions thereby highlighting the need to develop a greater understanding of the microbiology and physiology of marine diseases and ecosystems health, especially in the era of global warming.
Dopamine-grafted heparin as an additive to the commercialized carboxymethyl cellulose/styrene-butadiene rubber binder for practical use of SiOx/graphite composite anode
Authors: Lee Kukjoo , Lim Sanghyun , Go Nakgyu , Kim Jaemin , Mun Junyoung , Kim Tae-Hyun 
Published: July 2018

AbstractGraphite is used commercially as the active material in lithium ion batteries, frequently as part of a graphite/SiOx composite. Graphite is used in conjunction with SiOx to overcome the limited energy density of graphite, and to lessen the adverse effects of volume expansion of Si. However, electrodes based on graphite/SiOx composites can be made with only 3–5 wt % SiOx because of the increased failure of electrodes with higher SiOx contents. Here, we developed a new polymer binder, by combining dopamine-grafted heparin with the commercial binder carboxymethyl cellulose (CMC)/styrene butadiene rubber (SBR), in order to more effectively hold the SiOx particles together and prevent disintegration of the electrode during charging and discharging. The crosslinking using acid-base interactions between heparin and CMC and the ion-conducting sulfonate group in heparin, together with the strong adhesion properties of dopamine, yielded better physical properties for the dopamine-heparin-containing CMC/SBR-based electrodes than for the commercial CMC/SBR-based electrodes, and hence yielded excellent cell performance with a retention of 73.5% of the original capacity, a Coulombic efficiency of 99.7% at 150 cycles, and a high capacity of 200 mAh g−1 even at 20 C. Furthermore, a full cell test using the proposed electrode material showed stable cell performance with 89% retention at the 150th cycle.
Storm surge variation along the coast of the Bohai Sea
Authors: Feng Jianlong , Li Delei , Li Yan , Liu Qiulin , Wang Aimei 
Published: July 2018

AbstractThe present study mainly investigates the storm surge variations at different temporal scales using hourly tide gauge data in the Bohai Sea. The seasonal variation, inter-decadal variation, long-term trend and the tide-surge interaction were analyzed separately. The results show that the storm surges in the southwest Bohai Sea are larger than those in the north. The storm surges were more serious in winter (Oct. to Mar.) than in summer half of the year. Significant inter-decadal variations exist in the Bohai Sea, and the extreme storm surge events have been intensifying since 2010. Storm surge intensities at three of the tide gauges (Qinhuangdao, Huludao and Tanggu) exhibited a decreasing trend from 1980 to 2016, with trends significant at the 95% level at Qinhuangdao and Tanggu. Significant tide-surge interactions were observed at all four tide gauges. The tide-surge interaction that results in peak surges mostly occurs during the flood and ebb tides. There is a statistically significant negative correlation between storm surge intensity and the Arctic Oscillation (AO) at Longkou and Tanggu, while there is a significantly positive correlation between storm surge intensity and the Siberian High (SH) at Huludao, Qinhuangdao and Tanggu. A linear regression analysis revealed that the variations of the AO and SH explained 19–48% of the variations in the storm surge intensity in the Bohai Sea.
Palmitate-induced lipotoxicity is crucial for the pathogenesis of nonalcoholic fatty liver disease in cooperation with gut-derived endotoxin
Authors: Ogawa Yuji , Imajo Kento , Honda Yasushi , Kessoku Takaomi , Tomeno Wataru , Kato Shingo , Fujita Koji , Yoneda Masato , Saito Satoru , Saigusa Yusuke , Hyogo Hideyuki , Sumida Yoshio , Itoh Yoshito , Eguchi Kosei , Yamanaka Takeharu , Wada Koichiro , Nakajima Atsushi 
Published: July 2018

AbstractAlthough previous studies have indicated important roles of palmitate, a saturated fatty acid, in the pathogenesis of nonalcoholic fatty liver disease (NAFLD), it remains unclear how palmitate contributes to inflammation and fibrosis in the liver. Administration of palmitate in high fat diet (HFD)-fed but not basal diet (BD)-fed mice resulted in an increase in serum alanine aminotransferase (ALT) levels. Surprisingly, combined administration of very low dose lipopolysaccharide in palmitate-treated mice led to a marked increase in serum ALT levels despite BD-fed conditions. Administration of palmitate alone in BD-fed mice caused inflammatory cell infiltration and liver fibrosis mediated by the toll-like receptor 4 pathway without ALT elevation. In addition, a significant correlation between serum free fatty acid levels and liver fibrosis stage was observed in patients with NAFLD. These results indicate that palmitate may play crucial roles in the pathogenesis of NAFLD in the presence of gut-derived endotoxin.
Super-wide viewing-zone holographic 3D display using a convex parabolic mirror
Authors: Sando Yusuke , Satoh Kazuo , Kitagawa Takahiro , Kawamura Makoto , Barada Daisuke , Yatagai Toyohiko 
Published: July 2018

AbstractTo enlarge both horizontal (azimuthal) and vertical (zenithal) viewing zones simultaneously, a convex parabolic mirror is placed after passing through the hologram. Viewers perceive a three-dimensional (3D) object inside the parabolic mirror as a virtual image by capturing the wavefront radially reflected from the parabolic mirror. The optical experiment using the convex parabolic mirror has demonstrated an extremely wide viewing zone with an azimuthal range of 180° and zenithal range of 90°. The viewing zone and the shape of the parabolic surface are analyzed. The hologram is designed considering the parabolic mirror reflection, and its diffraction calculation method based on Fermat’s principle is also proposed.
Complementary intestinal mucosa and microbiota responses to caloric restriction
Authors: Duszka Kalina , Ellero-Simatos Sandrine , Ow Ghim Siong , Defernez Marianne , Paramalingam Eeswari , Tett Adrian , Ying Shi , König Jürgen , Narbad Arjan , Kuznetsov Vladimir A. , Guillou Hervé , Wahli Walter 
Published: July 2018

AbstractThe intestine is key for nutrient absorption and for interactions between the microbiota and its host. Therefore, the intestinal response to caloric restriction (CR) is thought to be more complex than that of any other organ. Submitting mice to 25% CR during 14 days induced a polarization of duodenum mucosa cell gene expression characterised by upregulation, and downregulation of the metabolic and immune/inflammatory pathways, respectively. The HNF, PPAR, STAT, and IRF families of transcription factors, particularly the Pparα and Isgf3 genes, were identified as potentially critical players in these processes. The impact of CR on metabolic genes in intestinal mucosa was mimicked by inhibition of the mTOR pathway. Furthermore, multiple duodenum and faecal metabolites were altered in CR mice. These changes were dependent on microbiota and their magnitude corresponded to microbial density. Further experiments using mice with depleted gut bacteria and CR-specific microbiota transfer showed that the gene expression polarization observed in the mucosa of CR mice is independent of the microbiota and its metabolites. The holistic interdisciplinary approach that we applied allowed us to characterize various regulatory aspects of the host and microbiota response to CR.
MiR-146a induction by cyanobacterial lipopolysaccharide antagonist (CyP) mediates endotoxin cross-tolerance
Authors: Molteni Monica , Bosi Annalisa , Saturni Vincenzo , Rossetti Carlo 
Published: July 2018

AbstractEndotoxin tolerance is a phenomenon characterized by a reduced capacity of monocytes and macrophages to respond to repeated stimulation with lipopolysaccharide (LPS) which has been suggested to represent a way of controlling the intensity and duration of innate immune response. During endotoxin tolerance, monocytes undergo functional re-programming primarily by epigenetic regulation. Recently, micro-RNA (miR)-146a has been demonstrated to be the major player of the negative regulation of the pro-inflammatory response, affecting TNF-α production. In this study, we have employed CyP, a cyanobacterial LPS antagonist acting on TLR4-MD2 complex, for priming human monocytes and evaluating their response to a subsequent challenge withE.coliLPS. Results show that CyP is able to induce cross-tolerance toE.coliLPS by inhibiting TNF-α production. The mechanism of action is mediated by a specific induction of miR-146a and reduction of IRAK1 and TRAF6 expressions in human monocytes by CyP priming. Up-regulation of miR-146a by CyP alone, affects subsequent cell response in term of TNF-α production even when monocytes are incubated with other TLR ligands, as lipoteichoic acid (LTA), thus confirming miR-146a as a critical player mediating TNF-α regulation during cross-tolerance with CyP.
Skeletal muscle cutoff values for sarcopenia diagnosis using T10 to L5 measurements in a healthy US population
Authors: Derstine Brian A. , Holcombe Sven A. , Ross Brian E. , Wang Nicholas C. , Su Grace L. , Wang Stewart C. 
Published: July 2018

AbstractMeasurements of skeletal muscle cross-sectional area, index, and radiation attenuation utilizing clinical computed tomography (CT) scans are used in assessments of sarcopenia, the loss of skeletal muscle mass and function associated with aging. To classify individuals as sarcopenic, sex-specific cutoffs for ‘low’ values are used. Conventionally, cutoffs for skeletal muscle measurements at the level of the third lumbar (L3) vertebra are used, however L3 is not included in several clinical CT protocols. Non-contrast-enhanced CT scans from healthy kidney donor candidates (age 18–40) at Michigan Medicine were utilized. Skeletal muscle area (SMA), index (SMI), and mean attenuation (SMRA) were measured at each vertebral level between the tenth thoracic (T10) and the fifth lumbar (L5) vertebra. Sex-specific means, standard deviations (s.d.), and sarcopenia cutoffs (mean-2 s.d.) at each vertebral level were computed. Associations between vertebral levels were assessed using Pearson correlations and Tukey’s difference test. Classification agreement between different vertebral level cutoffs was assessed using overall accuracy, specificity, and sensitivity. SMA, SMI, and SMRA L3 cutoffs for sarcopenia were 92.2 cm2, 34.4 cm2/m2, and 34.3 HU in females, and 144.3 cm2, 45.4 cm2/m2, and 38.5 HU in males, consistent with previously reported cutoffs. Correlations between all level pairs were statistically significant and high, ranging from 0.65 to 0.95 (SMA), 0.64 to 0.95 (SMI), and 0.63 to 0.95 (SMRA). SMA peaks at L3, supporting its use as the primary site for CT sarcopenia measurements. However, when L3 is not available alternative levels (in order of preference) are L2, L4, L5, L1, T12, T11, and T10. Healthy reference values reported here enable sarcopenia assessment and sex-specific standardization of SMA, SMI, and SMRA in clinical populations, including those whose CT protocols do not include L3.
Trichosanthes kirilowii lectin alleviates diabetic nephropathy by inhibiting the LOX1/NF-κB/caspase-9 signaling pathway
Authors: Lu Jiandong , Peng Jinting , Xiang Min , He Liangping , Wang Dongcai , Xiong Guoliang , Li Shunmin 
Published: July 2018

Trichosanthes kirilowii lectin (TKL) has been reported to exert hypoglycemic effects in alloxan-induced diabetic mice. However, there is no evidence showing that it helps to prevent diabetic nephropathy (DN). We used a high glucose (HG)-induced HK-2 cell model and a streptozocin (STZ)-induced Wistar rat model to investigate the effects of TKL on DN, as well as the mechanisms for those effects. Our results showed that TKL significantly increased the viability of HG-treated HK-2 cells and inhibited cell apoptosis. In vivo experiments demonstrated that TKL attenuated STZ-induced histopathological damage and the inflammatory response in rat kidney tissues. Pre-treatment of HK-2 cells or STZ-treated rats with polyinosinic acid (Poly IC), an inhibitor of lectin-like oxLDL receptor 1 (LOX1), blocked the protective effect of TKL against HG- or STZ-induced damage to kidney tissue, indicating that TKL might exert its effect via LOX1-mediated endocytosis. Additional results suggested that TKL inhibits the phosphorylation of IκB kinase β (IKKβ) and the nuclear factor-κB (NF-κB) inhibitor protein (IκBα), and thereby reduces the nuclear translocation of NF-κB (p65). ChIP assay data indicated that TKL markedly inhibits the binding of p65 to the CASP9 gene in HG-treated HK-2 cells, subsequently suppressing transcription of the CASP9 gene. In the dual-luciferase reporter assay, TKL significantly inhibited luciferase activity in cells co-transfected with p65 and a wild-type capase-9 construct instead of mutated caspase-9 constructs.
Taken together, our results show that TKL helps to protect against DN by inhibiting the LOX1/NF-κB/caspase-9 signaling pathway, suggesting TKL as a promising agent for treating DN.
The SLC34A2-ROS-HIF-1-induced up-regulation of EZH2 expression promotes proliferation and chemo-resistance to apoptosis in colorectal cancer
Authors: Li Xu , Xing Junjie , Wang Hantao , Yu Enda 
Published: July 2018

Abstract
Growing evidence has uncovered that SLC34A2 plays an evident role in the progression in several types of tumors. However, the biological function and underlying molecular mechanisms of SLC34A2 remain largely unknown. Here, we indicated that SLC34A2 expression was markedly increased in SW480 and HT29 cell line cells compared with that in normal colorectal epithelial cell line cells. Array analysis displayed that the expression of enhancer of zeste 2 (EZH2) decreased considerably when SLC34A2 was knocked down. We demonstrated that SLC34A2 induced EZH2 expression and activated its promoter activity. Serial 5′ deletion and site-directed mutagenesis revealed that the induction of EZH2 expression by SLC34A2 was dependent upon the hypoxia-inducible factor 1 (HIF-1)-2 binding site directly within EZH2 promoter. Moreover, HIF-1 activation was proved essential for SLC34A2-induced EZH2 expression. Reactive oxygen species (ROS) generation contributed to the stabilization of HIF-1α by leading to the binding of HIF-1α to the EZH2 promoter, which resulted in increased EZH2 expression. Additionally, we showed that the inhibition of both HIF-1α expression and ROS generation by YC-1 or BHA, respectively, decreased SLC34A2-induced EZH2 overexpression. Significantly, SLC34A2-induced EZH2 overexpression promoted the proliferation and chemo-resistance to apoptosis in colorectal cancer (CRC) cells in vitro and in vivo. Altogether, we conclude that the SLC34A2-ROS-HIF-1-induced overexpression of EZH2 promotes CRC cells proliferation and chemo-resistance to apoptosis. SLC34A2-ROS-HIF-1-EZH2 signaling pathway might serve as a novel therapeutic target against CRC.