bluuebunny/crossref_metadata_2025_split huggingface dataset:
Innovation for sustainable development in the food industry: Retro and forward‐looking innovation approaches to improve quality and healthiness
Authors: León‐Bravo Verónica , Moretto Antonella , Cagliano Raffaella , Caniato Federico 
Published: June 2019

AbstractSustainable development in the food industry requires companies to be more innovative with their products and supply chain as a strategy for developing healthier and higher quality food products, and achieving better economic, environmental, and social performance. A clear path to follow when companies decide to develop innovations for sustainability is still lacking, especially with a focus on quality and healthiness. This study analyzes six case studies in the food industry in Italy, identifying two innovative approaches towards sustainable development for quality and healthiness, namely, forward‐looking innovation and retro‐innovation. The first one represents a typical business model redesign oriented to sustainability, that is, developing new products and reconfiguring the supply chain processes for entering new market segments, applied mainly in smaller companies. The second one, indeed, implies a supply chain restructuring strategy while going back to traditional agricultural processes, especially applied in companies with standard or certified products. Both approaches target sustainability performances and allow an improvement of food quality and healthiness.
Nickel‐Based Transition Metal Nitride Electrocatalysts for the Oxygen Evolution Reaction
Authors: Tareen Ayesha Khan , Priyanga G. Sudha , Khan Karim , Pervaiz Erum , Thomas Tiju , Yang Minghui 
Published: June 2019

AbstractElectrocatalysis is an efficient and promising means of energy conversion, with minimal environmental footprint. To enhance reaction rates, catalysts are required to minimize overpotential. Alternatives to noble metal electrocatalysts are essential to address these needs on a large scale. In this context, transition metal nitride (TMN) nanoparticles have attracted much attention owing to their high catalytic activity, distinctive electronic structures, and enhanced surface morphologies. Nickel‐based materials are an ideal choice for electrocatalysts given nickel's abundance and low cost in comparison to noble metals. In this Minireview, advancements made specifically in Ni‐based binary and ternary TMNs as electrocatalysts for the oxygen evolution reaction (OER) are critically evaluated. When used as OER electrocatalysts, Ni‐based nanomaterials with 3 D architectures on a suitable support (e.g., a foam support) speed up electron transfer as a result of well‐oriented crystal structures and also assist intermediate diffusion, during reaction, of evolved gases. 2 D Ni‐based nitride sheet materials synthesized without supports usually perform better than 3 D supported electrocatalysts. The focus of this Minireview is a systematic description of OER activity for state‐of‐the‐art Ni‐based nitrides as nanostructured electrocatalysts.
A High‐Sensitivity 10‐Color Flow Cytometric Minimal Residual Disease Assay in B‐Lymphoblastic Leukemia/Lymphoma Can Easily Achieve the Sensitivity of 2‐in‐106and Is Superior to Standard Minimal Residual Disease Assay: A Study of 622 Patients
Authors: Tembhare Prashant R. , Subramanian PG Papagudi G. , Ghogale Sitaram , Chatterjee Gaurav , Patkar Nikhil V. , Gupta Avinash , Shukla Rahul , Badrinath Yajamanam , Deshpande Nilesh , Narula Gaurav , Rodrigues Pearl , Girase Karishma , Dhaliwal Dilshad , Prasad Maya , Shetty Dhanalaxmi , Banavali Shripad , Gujral Sumeet 
Published: June 2019

BackgroundFlow‐cytometric minimal residual disease (FC‐MRD) monitoring is a well‐established risk‐stratification factor in B‐lymphoblastic leukemia/lymphoma (‐B‐ALL) and is being considered as a basis for deintensification or escalation in treatment protocols. However, currently practiced standard FC‐MRD has limited sensitivity (up to 0.01%) and higher false MRD‐negative rate. Hence, a highly sensitive, widely applicable, and easily reproducible FC‐MRD assay is needed, which can provide a reliable basis for therapeutic modifications.MethodsA 10‐color high‐event analysis FC‐MRD assay was studied for the evaluation of MRD status at postinduction, (PI; day‐35), postconsolidation, (PC; day‐78), and subsequent follow‐up time‐points (SFU) in bone marrow samples from pediatric B‐ALL.ResultsOne‐thousand MRD samples (PI‐62.2%; PC‐26.5%; and SFU‐11.3%) from 622 childhood B‐ALL patients were studied. High‐event analysis was performed with median 4,452,000 events (range, 839,000 to 8,866,000 events) and >4 million events in 71% samples. MRD was measurable in 43.2% of PI‐samples, in 29.4% PC‐samples, and in 32.7% SFU‐samples. To simulate comparison with standard FC‐MRD, we reanalyzed MRD results gating only first 500,000 and first 1000,000 events in 122 PI‐MRD positive samples with MRD levels <0.02%. Of these samples gated for 500,000 events and 1000,000 events, 32% and 21.3% were found to be falsely MRD‐negative, respectively.ConclusionsWe report an easily reproducible high‐sensitivity 10‐color FC‐MRD assay with the sensitivity of 2‐in‐106(0.0002%). It allowed the detection of low‐level MRD in samples, which could have been reported negative using the standard FC‐MRD with limited event analysis. Thus, this high‐sensitivity MRD‐methodology can provide a reliable basis for therapeutic modifications in B‐ALL. © 2019 International Clinical Cytometry Society
Essential waters: Young bull sharks in Fiji's largest riverine system
Authors: Glaus Kerstin B. J. , Brunnschweiler Juerg M. , Piovano Susanna , Mescam Gauthier , Genter Franziska , Fluekiger Pascal , Rico Ciro 
Published: June 2019

AbstractCoastal and estuarine systems provide critical shark habitats due to their relatively high productivity and shallow, protected waters. The young (neonates, young‐of‐the‐year, and juveniles) of many coastal shark species occupy a diverse range of habitats and areas where they experience environmental variability, including acute and seasonal shifts in local salinities and temperatures. Although the location and functioning of essential shark habitats has been a focus in recent shark research, there is a paucity of data from the South Pacific. In this study, we document the temporal and spatial distribution, age class composition, and environmental parameters of young bull sharks (Carcharhinus leucas) in the Rewa, Sigatoka, and Navua Rivers, Fiji's three largest riverine systems. One hundred and seventy‐two young bull sharks were captured in fisheries‐independent surveys from January 2016 to April 2018. The vast majority of the captures were neonates. Seasonality in patterns of occurrence of neonate individuals suggests a defined parturition period during summer. Environmental parameters between the Rewa and the Sigatoka River differed significantly, as did the recorded young bull sharks abundance. According to the surveys, young bull sharks occur in all three rivers with the Rewa River likely representing essential habitat for newly born bull sharks. These results enhance the understanding of bull shark ecology in Fiji and provide a scientific basis for the implementation of local conservation strategies that contribute to the protection of critical habitats.
Analysis of antioxidant effect of two tripeptides isolated from fermented grains (Jiupei) and the antioxidative interaction with 4‐methylguaiacol, 4‐ethylguaiacol, and vanillin
Authors: Jiang Yunsong , Zhao Dongrui , Sun Jinyuan , Luo Xuelian , Li Hehe , Sun Xiaotao , Zheng Fuping 
Published: June 2019

AbstractJiupei (fermented grains) is the raw material for Baijiu distillation. The antioxidant activities of peptides Val‐Asn‐Pro (VNP) and Tyr‐Gly‐Asp (YGD) identified from Jiupei were evaluated according to in vitro chemical assays (e.g., 2,2′‐azino‐bis (3‐ethylbenzothiazoline‐6‐sulfonic acid) diammonium salt, 1,1‐diphenyl‐2‐picrylhydrazyl, and oxygen radical absorbance capacity) and 2,2′‐azobis (2‐methylpropionamide)‐dihydrochloride‐activated HepG2 cell model. The interaction on antioxidant activities between peptides (VNP and YGD) and three functional phenols (4‐methylguaiacol, 4‐ethylguaiacol, and vanillin) which were found in Baijiu was also measured. On the basis of the results, two peptides exhibited strong antioxidant ability in oxygen radical absorbance capacity (ORAC) assay. Furthermore, they suppressed the generation of reactive oxygen species. The intracellular antioxidant enzymatic system and nonenzymatic system were also regulated by VNP and YGD. In addition, it was confirmed that partial auxo‐action between peptides and phenols appeared mostly in chemical assays. The findings above might indicate that VNP and YGD are potent natural antioxidants in Jiupei even in Baijiu through distillation process and lay the foundation for illustrating the interactions among different functional substances in Baijiu.
Accuracy evaluation of automated electrochemiluminescence immunoassay for everolimus and sirolimus compared to liquid chromatography‐tandem mass spectrometry
Authors: Lee Eun Jin , Kim Hyun‐Ki , Ahn Sunyoung , Lee Woochang , Kim Hyun Soo , Chun Sail , Min Won‐Ki 
Published: June 2019

AbstractBackgroundWe evaluated the analytical performance of a newly developed electrochemiluminescence immunoassay for everolimus and sirolimus compared to that of liquid chromatography‐tandem mass spectrometry (LC‐MS/MS).MethodsAccording to Clinical and Laboratory Standards Institute guidelines, the analytical performance including precision, recovery, linearity, and carryover was evaluated. For correlation evaluation, the results of Elecsys® analysis of everolimus and sirolimus were compared with those of LC‐MS/MS using 120 samples from patients treated with everolimus or sirolimus.ResultsThe within‐run and total imprecision values were as follows: 2.3%‐4.5% and 4.5%‐6.4% for the everolimus assay; 3.3%‐4.8% and 4.7%‐8.1% for the sirolimus assay, respectively. The measured concentration was linear over the range of 0.718‐27.585 ng/mL for everolimus analysis and 0.789‐26.880 ng/mL for sirolimus analysis (all R2 > 0.99). Recovery was 93.5%‐105.5% for the everolimus assay and 99.2%‐109.1% for the sirolimus assay (except lowest levels). Carryover was −1.09% for the everolimus assay and −0.12% for the sirolimus assay. The results of the two chemiluminescence immunoassays showed acceptable correlations with those of LC‐MS/MS (R = 0.9585 and R = 0.9799, respectively). The two immunoassays showed slightly proportional biases compared to LC‐MS/MS.ConclusionElecsys® Everolimus and Sirolimus assays showed acceptable analytical performance in precision, linearity, and correlation compared to LC‐MS/MS These methods can be adopted in the clinical laboratory for rapid therapeutic drug monitoring of patients who require treatment with immunosuppressants.
Serum ubiquitin C‐terminal hydrolase L1 predicts cognitive impairment in patients with acute organophosphorus pesticide poisoning
Authors: Pang Li , Liu Junlan , Li Wei , Xia Yan , Xing Jihong 
Published: June 2019

AbstractBackgroundTo assess the usefulness of serum C‐terminal hydrolase L1 (UCH‐L1) level as a biomarker for predicting cognitive impairment in patients with acute organophosphorus pesticide poisoning (AOPP).MethodsTwo hundred and seven adult patients with AOPP were included in this study. Serum UCH‐L1 levels were assessed on admission (Day 1 postpoisoning) and on Days 3 and 7 postpoisoning. The associations between serum UCH‐L1 levels, other clinical predictors, and cognitive function evaluated on Day 30 postpoisoning were investigated.ResultsOn multivariate analysis, serum UCH‐L1 levels on admission (odds ratio [OR] 1.889, 95% confidence interval [CI] 1.609‐3.082, P = 0.002) and 24‐hour APACHE II score (OR 1.736, 95% CI 1.264‐3.272, P = 0.012) were independent predictors of cognitive impairment on Day 30 postpoisoning. Based on the receiver operating characteristic curve, serum UCH‐L1 levels >5.9 ng/mL on admission predicted cognitive impairment on Day 30 postpoisoning with 86.1% sensitivity and 72.5% specificity (area under the curve, 0.869; 95% CI 0.815‐0.923). On admission [8.51 (6.53‐10.22) ng/mL vs 4.25 (2.57‐6.31) ng/mL, P < 0.001] and Day 3 [9.31 (7.92‐10.98) ng/mL vs 3.32 (2.25‐5.13) ng/mL, P < 0.001] and Day 7 [4.96 (3.28‐7.26) ng/mL vs 2.27 (1.55‐3.24) ng/mL, P < 0.001] postpoisoning, serum UCH‐L1 concentration was significantly higher in patients that developed cognitive impairment compared to those that did not.ConclusionThis study demonstrates that serum UCH‐L1 level has potential as a novel biomarker for predicting cognitive impairment 30 days after AOPP.
The association between the ApoE polymorphisms and the MRI‐defined intracranial lesions in a cohort of southern China population
Authors: Li Zhuoran , Yang Na , Lei Xiuxia , Lin Chuying , Li Nan , Jiang Xinqing , Wei Xinhua , Xu Banglao 
Published: June 2019

AbstractBackgroundThe apolipoprotein E (APOE) ε4 allele is considered as a risk factor for Alzheimer's disease (AD). However, the association of APOE allele with MRI evidence of intracranial lesions has not been well understood.MethodsQuantitative real‐time PCR was performed to detect the APOE genotype; MRI was examined for intracranial lesions. Their association was evaluated in a cohort of 226 AD patients and 2607 healthy individuals in southern China.ResultsThe frequencies of ε2, ε3, and ε4 alleles were 8.0%, 82.9%, and 9.1% in the whole study population. The frequency of APOE‐ε4 allele was significantly higher in the AD subjects than that in the control group (14.4% vs 8.6%, P < 0.001). We found that brain atrophy occurred at a rate of 12.3% in ε4 allele group vs 8.5% in non‐ε4 genotype group, with a significance of P = 0.008. Severe brain atrophy occurred at a rate of 1.0% in ε4 allele group vs 0.2% in non‐ε4 genotype group (P = 0.011). The individuals carrying APOE ε4/ε4 had an odds ratio (OR) of 7.64 (P < 0.01) for developing AD, while the APOE ε3/ε4 gene carriers had an OR of 1.47 (P = 0.031) and the OR in APOE ε2/ε3 carriers is 0.81 (P = 0.372). Interestingly, we found that the risk of ε4/ε4 allele carrier developing AD was significantly higher in male (P < 0.001) than female (P = 0.478).ConclusionCompared to ε2 and ε3 alleles, the presence of APOE‐ε4 allele might increase the risk for AD in a dose‐dependent manner in southern China. Moreover, the presence of APOE‐ε4 allele results in a higher incidence of brain atrophy.
Morphochemical characterization, microhardness, water sorption, and solubility of regular viscosity bulk fill and traditional composite resins
Authors: Melo Rafaela A. , Bispo Andressa de Souza Leite , Barbosa Gustavo A. S. , Galvão Marilia R. , de Assunção Isauremi V. , Souza Rodrigo O de Assunção , Borges Boniek C. D. 
Published: June 2019

AbstractObjectiveTo evaluate the morphology of filler particles, chemical composition, microhardness (MH), water sorption (WSp), and solubility (WSl) of a regular viscosity bulk fill and traditional composite resins.MethodsEighty samples (Ø:5 mm; height: 4 mm) were prepared according to the factors “composite” (Aura/SDI, FiltekZ250 XT/3M, Aura Bulk Fill/SDI, and Filtek Bulk Fill/3M) and “filling technique” (incremental and bulk) (n = 10). Vickers MH was measured on the top and bottom surfaces of each samples, and then WSp and WSl were obtained by means of mass gain and loss. Morphology of filler particles and chemical characteristics of composites were evaluated by scanning electron microscopy (SEM) and energy dispersity spectroscopy (EDS) in additional samples (n = 1/group). Data were analyzed using two‐ and three‐way ANOVA and Tukey's tests (p < .05).ResultsNo significant difference was found for WSp among the groups. Comparing composites in the incremental technique, Aura bulk fill composite showed lower WSI than the other materials and in the bulk fill technique, Filtek Bulk Fill showed the lowest value. Filtek Bulk Fill showed higher MH than the other composites on the bottom surface when samples were produced by bulk filling.ConclusionThe composites presented good physical properties, but the bulk fill ones showed better results for the bottom microhardness and solubility, although chemical elements and morphology were similar in general.
The new route for realization of 1‐μm‐pixel‐pitch high‐resolution displays
Authors: Choi Ji Hun , Yang Jong‐Heon , Pi Jae‐Eun , Hwang Chi‐Young , Kim Yong‐Hae , Kim Gi Heon , Kim Hee‐Ok , Hwang Chi‐Sun 
Published: June 2019

AbstractA new pixel structure for the realization of a 1‐μm‐pixel‐pitch display was developed. This structure, named vertically stacked thin‐film transistor (VST), was based on the conventional back‐channel etched thin‐film transistor (TFT), but all the layers except the horizontal gate line were vertically stacked on the embedded data line, enabling the implementation of high‐resolution display panels. The VST device with a channel length of 1 μm showed a high field effect mobility of more than 50 cm2/Vs and low subthreshold slope of 78 mV per decade. It also shows a high uniform electrical characteristic over the entire 6‐in. wafer. The development of a new pixel architecture is expected to enable the implementation of 1‐μm‐pixel‐pitch high‐resolution displays such as spatial light modulators for digital holograms.
Determination of five nucleosides by LC‐MS/MS and the application of the method to quantify N6‐methyladenosine level in liver messenger ribonucleic acid of an acetaminophen‐induced hepatotoxicity mouse model
Authors: Liu Ruijuan , Zhao Fang , Wei Jingyao , Yu Pei , Zhang Ji , Wang Yueqin , Li Zeyun , Zhang Jiali , Zhang Xiaojian , Tian Xin 
Published: June 2019

AbstractRibonucleic acid N6‐methyladenosine methylation plays an important role in a variety of biological processes and diseases. Acetaminophen‐induced hepatotoxicity is one of the major challenges faced by clinicians. To date, the link between N6‐methyladenosine and acetaminophen‐induced hepatotoxicity has not been studied. In this study, a simple ultra high performance liquid chromatography with tandem mass spectrometry method was developed for the simultaneous determination of five nucleosides (adenosine, uridine, cytidine, guanosine, and N6‐methyladenosine) in messenger ribonucleic acid. After enzymatic digestion of messenger ribonucleic acid, the nucleosides sample was separated on an Acquity UPLC column with gradient elution using methanol and 0.02% formic acid water, and detected by a Qtrap 4500 mass spectrometer with an electrospray ionization mode. The method was validated over the concentration ranges of 4–800 ng/mL for adenosine, uridine, cytidine, and guanosine and 0.1–20 ng/mL for N6‐methyladenosine. It was successfully applied to the determination of N6‐methyladenosine levels in liver messenger ribonucleic acid in an acetaminophen‐induced hepatotoxicity mouse model and a control group. This study offers a method for the determination of nucleoside contents in epigenetic studies and constitutes the first step toward the investigation of ribonucleic acid methylation in acetaminophen‐induced hepatotoxicity, which will facilitate the elucidation of its mechanism.
Phthalate sum determination in farmland soils using high‐performance liquid chromatography with tandem mass spectrometry
Authors: Han Yangying , Ren Yanmei , Duan Jiahui , Liu Laping , Gao Jinming , Liu Shuhui 
Published: June 2019

AbstractA selective and low organic‐solvent‐consuming method of sample preparation combined with high‐performance liquid chromatography and tandem mass spectrometry is introduced for phthalate sum analysis in farmland soil. Sample treatment involves a one‐step hydrolysis of phthalates using methanol and alkaline and tetrabutylammonium bromide for 20 min at 80℃. Then, the resulting phthalic acid in the acidified hydrolysate is extracted using an octanol‐based supramolecular solvent without purification. Under optimized conditions, the correlation coefficients were 0.992–0.999 and standard errors (Sy/x) were 0.018–0.138 for calibration curves within the range of 50–2000 ng/mL. No obvious matrix effect occurred between the pure supramolecular solvent and soil extract. The recovery rates ranged from 91 to 107% with the relative standard deviation ranging from 0.5–7.3%. Intra‐ and interday repeatability, expressed as relative standard deviation, was less than 8.0 and 11.0%, respectively. The detected limit was 2.49 nmol/g, and the quantification limit was 3.64 nmol/g. Fifteen soil samples were analysed, and the background corrected phthalate sum ranged from 1.44 to 120 nmol/g.
Temperature‐dependent rate constant for the reaction of F atoms with HNO3
Authors: Bedjanian Yuri 
Published: June 2019

AbstractThe kinetics of the reaction of F atom with HNO3, source of NO3 radicals widely used in laboratory studies, has been investigated at nearly 2.7 mbar total pressure of helium over a wide temperature range, T = 220‐700 K, using a low‐pressure discharge flow reactor combined with an electron impact ionization quadrupole mass spectrometer. The rate constant of the reaction F + HNO3 → NO3 + HF (1) was determined using both relative rate method and absolute measurements under pseudo–first‐order conditions, monitoring the kinetics of F‐atom consumption in excess of HNO3, k1 = (8.2 ± 0.4) × 10−12 exp((315 ± 15)/T) cm3 molecule−1 s−1 (where the uncertainties represent precision at the 2σ level, the estimated total uncertainty on k1 being 15% at all temperatures). The reaction rate constant was found to be in excellent agreement with the only previous temperature‐dependent study. Experiments on detection of the reaction product, HF, have shown that NO3 and HF forming channel of the title reaction is the dominant, if not unique, on the whole temperature range of the study.
Two Specific Sulfatide Species Are Dysregulated during Renal Development in a Mouse Model of Alport Syndrome
Authors: Gessel Megan M. , Spraggins Jeffrey M. , Voziyan Paul A. , Abrahamson Dale R. , Caprioli Richard M. , Hudson Billy G. 
Published: June 2019

AbstractAlport syndrome is caused by mutations in collagen IV that alter the morphology of renal glomerular basement membrane. Mutations result in proteinuria, tubulointerstitial fibrosis, and renal failure but the pathogenic mechanisms are not fully understood. Using imaging mass spectrometry, we aimed to determine whether the spatial and/or temporal patterns of renal lipids are perturbed during the development of Alport syndrome in the mouse model. Our results show that most sulfatides are present at similar levels in both the wild‐type (WT) and the Alport kidneys, with the exception of two specific sulfatide species, SulfoHex‐Cer(d18:2/24:0) and SulfoHex‐Cer(d18:2/16:0). In the Alport but not in WT kidneys, the levels of these species mirror the previously described abnormal laminin expression in Alport syndrome. The presence of these sulfatides in renal tubules but not in glomeruli suggests that this specific aberrant lipid pattern may be related to the development of tubulointerstitial fibrosis in Alport disease.