bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nitric oxide impacts bovine sperm capacitation in a cGMP‐dependent and cGMP‐independent manner
Authors: Maciel Valter Luiz , Caldas‐Bussiere Maria Clara , Marín Diego Fernando Dubeibe , Paes de Carvalho Carla Sobrinho , Quirino Celia Raquel , Leal Ana Carolina de Macedo Soares 
Published: September 2019

AbstractWe aimed to elucidate whether NO acts in in vitro sperm capacitation in bovine via cGMP/PKG1 pathway. For this, cryopreserved bovine sperm were capacitated in vitro with 20 µg/ml heparin (Control) plus treatments: 1 mM L‐arginine (L‐arg, NO precursor), 50 µM Rp‐8‐Bromo‐β‐phenyl‐1,N2‐ethenoguanosine‐3′,5′‐cyclic monophosphorothioate (Rp‐8‐Br‐cGMPS, selective inhibitor of the binding site for cGMP in PKG1), 1 mM 2‐Phenyl‐4,4,5,5‐tetramethylimidazoline‐1‐oxyl 3‐oxide (PTIO, NO scavenger), and the combinations of L‐arg + RP‐8‐Br‐cGMPS and L‐arg + PTIO. Sperm motility and vigour were determined by phase‐contrast microscopy, capacitation status by chlortetracycline staining, and the intracellular concentration of cGMP was measured by ELISA. Data were subjected to analysis of variance and means compared with SNK test at 5% probability. Motility and vigour were lower in sperm treated with PTIO when compared to Control and other treatments (p < .05). The L‐arg treatment showed the highest percentage of capacitated sperm when compared to the Control and other treatments (Rp‐8‐Br‐cGMPS, L‐arg + Rp‐8‐Br‐cGMPS and PTIO) (69.8 ± 3.4%, 51.2 ± 3.0, 51.1 ± 2.1, 51.2 ± 3.0 and 45.5 ± 2.7, respectively) (p < .05). The capacitation ratio (%) was lower in treatments with Rp‐8‐Br‐cGMPS, L‐arg + Rp‐8‐Br‐cGMPS and PTIO, respectively (p < .05). Lastly, cGMP concentration (pmol/ml) was lower in PTIO and L‐arg + PTIO (1.3 ± 0.3 and 1.6 ± 0.4) and was higher in Rp‐8‐Br‐cGMPS and L‐arg + Rp‐8‐Br‐cGMPS (3.7 ± 0.4 and 4.0 ± 0.5) treatments. We showed that during in vitro capacitation of cattle: (a) NO influences sperm motility and vigour; (b) NO is associated with cGMP synthesis through two independent pathways and (c) the cGMP/PKG1 pathway has a partial role in sperm capacitation and does not involve the L‐arg/NO.
Inspiring Interest in and Curiosity for Physics: Looking Beyond the Stars
Authors: Chang Ngee-Pong 
Published: September 2019

As physics educators, our mission is not only to impart the knowledge of physics but to inspire interest in physics as well. [Formula: see text]In this article, I hope to open the eyes of some students who are interested and curious enough to want to look beyond the daily physics principles and think about the universe. [Formula: see text]The story of Newton, Hooke, and Halley provides a context and lesson for future generations of physicists on how to think outside the box, and look beyond the stars.
Acoustic Nanomotors for Detection of Human Papillomavirus–Associated Head and Neck Cancer
Authors: Qualliotine Jesse R. , Bolat Gulcin , Beltrán‐Gastélum Mara , de Ávila Berta Esteban‐Fernández , Wang Joseph , Califano Joseph A. 
Published: September 2019

ObjectiveHuman papillomavirus (HPV)–associated oropharyngeal cancer (OPC) is a lethal disease with increasing incidence; however, technologies for early detection are limited. Nanomotors are synthetic nanostructures that can be powered by different mechanisms and functionalized for specific applications, such as biosensing. The objective of this investigation was to demonstrate an in vitro proof of concept for a novel nanomotor‐based cancer detection approach toward in vivo detection of HPV‐OPC.Study DesignIn vitro cell line incubated with ultrasound‐propelled nanomotors.SettingBasic science and engineering laboratories.Subjects and MethodsUltrasound‐powered gold nanowire nanomotors were functionalized with graphene oxide and dye‐labeled single‐stranded DNA for the specific intracellular detection of HPV16 E6 mRNA transcripts. Nanomotors were incubated with HPV‐positive or HPV‐negative human OPC cells under static conditions or with an applied ultrasound field for 15 minutes. The resulting intracellular fluorescence was assessed with fluorescence microscopy and analysis software.ResultsNanomotors incubated with RNA extracted from HPV‐positive OPC cells resulted in 60.7% of maximal fluorescence recovery, while incubation with RNA extracted from HPV‐negative cells produced negligible fluorescence. Nanomotor incubation with intact HPV‐negative cells produced minimal fluorescence (0.01 au), while incubation with HPV‐positive cells produced a detectable signal (0.43 au) under static conditions and had 2.3‐times greater intensity when powered with ultrasound.ConclusionAcoustically powered nanomotors can successfully identify HPV16 E6 mRNA transcripts extracellularly and within intact cells. This work represents the first step toward a novel, practical approach to address the challenge of visually detecting HPV‐OPC in real time.
CO2-ak bultzatutako gizarte baterantz: landareetatik ikasten
Authors: Huizi-Rayo Uxua , San Sebastian Eider , Cepeda Javier 
Published: September 2019

Gaur egungo energia-beharra dela eta, energia-iturri fosilak agortu eta CO2- isuriak hazten ari dira. Horrek energia-iturri alternatiboekin bultzatutako gizartearen ideiari indarra ematen dio. CO2-emisioen papera klima-aldaketan aski ezaguna da. Horregatik, kutsatzaile horren birziklapenak energia-beharra eta klima-aldaketa erregula ditzakeela pentsatzen da. CO2 eraldatzeko prozesu bat baino gehiago badaude ere, gehienetan tenperatura eta boltaje oso altuak behar dira, erredukzio fotokatalitikoaren kasuan izan ezik. Horrek landareen fotosintesia simulatzen du, baina, prozesuari bere etekin guztia ateratzeko, fotokatalizatzaileak hobetu behar dira. MOFak metalak eta molekula organikoak koordinatuz osatutako material nanoporotsu mota bat dira, adsortzioaren bidez molekula txikiak harrapatu ditzaketen zulo edo kanalak dituztenak. MOFaren diseinu egokiarekin, metalak eta ligandoak ondo aukeraturik, material horiek CO2-aren erredukzio fotokatalitikoa burutzeko gai izan daitezke.
Fluorescence anti-Stokes de l'Er3+ et du Tm3+ par transfert d'energie
Authors: Auzel F. , Deutschbein O. 
Published: September 2019

Abstract
Antistokes fluorescence in the green and blue region, excited by infrared radiation (λ=0.97μm) is observed with a relatively high efficiency in substances which contain not only a luminescent activator (Er3+ or Tm3+), but also a sensitizer which has a strong infrared absorption (Yb3+). Polycrystalline mixed tungstates and germanate glasses have been studied. The green fluorescence of Er3+ increases as a square-law function of the excitation intensity, the blue fluorescence of Tm3+ as a cubic function. This effect has been used to build an optical amplifier which has served to measure the excitation spectra. The mechanism of excitation is the following: Absorption of the infrared radiation by the sensitizer, followed by energy transfers to the luminescent activator, which is excited stepwise up to the initial level of luminescence. Oscillator strengths for some transitions between excited levels have been calculated.
Excess Electrons and Positive Charge Carriers in Liquid Methane. I
Authors: Bakale George , Schmidt Werner F. 
Published: September 2019

Abstract
The drift velocities of radiation-induced excess electrons and positive charge carriers in liquid methane were measured at different electric field strengths and several temperatures. For the excess electrons the drift velocity increases up to 1.5 kV cm-1 proportional to the electric field strength and a mobility of (400±50) cm2 V-1 s-1 at T = 111 °K was obtained. Above 1.5 kV cm-1 the drift velocity varies with E½. The temperature coefficient of the mobility is negative. For the positive charge carriers the measurements were carried out up to electric field strengths of 50 kV cm-1 and the drift velocity remained proportional to the field giving a mobility of (2.5 ± 0.5) · 10-3 cm2 V-1 s-1 at 7 = 111 °K. The mobility increased with increasing temperature.
The reaction of excess electrons with oxygen was also studied and a rate constant of 8.4 · 1011 l mole-1 s-1 was obtained.
Exploring Learners Experience of Gamified Practicing: For Learning or for Fun?
Authors: Dicheva Darina , Irwin Keith , Dichev Christo 
Published: September 2019

Gamification in education primarily aims at increasing learners’ motivation to engage in given learning activities and thereby promote desired learning behaviors. Despite its potential and the increasing number of gamified systems their success is still limited due to the fact that gamifying learning is a challenging process. Part of the challenge stems from an insufficient understanding of the motivational mechanisms of gamification on learners’ experience and from the insufficient attention to factors contributing to gameful experiences and promoting sustainable engagement. In addition, there is limited empirical evidence on motivational influences driving students to participate in gamified learning activities. Another factor slowing down the progress of successfully gamified applications is the scarcity of tools that would enable instructors to design and implement gamified activities as part of their instructional strategy. In an attempt to address these challenges, the present paper examines the difficulties inherent in the gamification of educational activities and how the OneUp gamification platform can support instructors in overcoming them with the mechanisms it provides for affording gameful experiences and sustainable engagement. To add to the understanding of the motivational influences towards gamified learning activities, the paper presents a preliminary study of student motivation to use OneUp for out-of-class practicing in a gamified Data Structures course. The outcomes of this study are based on data collected through a focus group, survey data, and system logs.
MANUFACTURING TECHNIQUE OF METAL CERAMIC RESTORATION WITH IMPLANT-ABUTMENT AT GINGIVAL RESORPTION CASE
Authors: Paramarta Galih , Inayati Eny 
Published: September 2019

Background: The low levels of patient satisfaction and confidence in the restoration of the gingival resorption results in impaired quality of life in both functional and psychosocial aspects. It is necessary to manufacture a dental restoration to restore the mastication, phonetic, and aesthetic functions associated with soft tissue repair. Manufacture of gingiva ceramic restorations which are believed to be an alternative to restore the aesthetic function and harmonization of the patient’s teeth. In terms of manufacturing techniques, this restoration has a level of difficulty in achieving a balance between “White aesthetic” and “aesthetic pink”. Objective: This study aims to provide knowledge to the reader of Manufacturing Technique of Metal Ceramic Restoration with Implant-Abutment at Gingival Resorption Case. Reviews: Gingiva ceramic restorations can be used to create esthetic implant-supported restorations when bone and gingival tissues are deficient and surgical replacement of such tissues is not possible. Dental technicians should be able to analyze the three-dimensional shapes of gingival anatomy, color, texture, smile components, the balance of the “pink aesthetic” and “white aesthetic”. Conclusion: Manufacturing Technique of Metal Ceramic Restoration with Implant-Abutment at Gingival Resorption Case including the manufacture of metal coping, oxidation, application of opaque coating, application of the first “white aesthetic” ceramics (Opaque Dentine, Dentine, Enamel and transpa on crown coping restorations), Application of opaque gingiva layer, gingival ceramics build-up "pink aesthetics”, first firing, followed by correction build-up, and second firing, and ends with the glazing process.
TRANSLATION STUDY OF REFUSAL STRATEGY IN BEAUTIFUL MALICE NOVEL (A PRAGMATIC APPROACH)
Authors: Soe Wyut Yee 
Published: September 2019

<p class="IsiAbstrakabstractcontent">The study aims to know translation technique and translation quality of refusal strategy in Beautiful Malice novel. It was descriptive qualitative method with embedded case study. The data were obtained through content analysis and focus group discussion. They were then analyzed into domain, taxonomy and componential analysis to formulate cultural theme. The result shows that writer uses indirect and direct refusal strategy in which indirect one is mostly used. Besides, translator transfers those refusal strategies by using established equivalence, modulation, explicitation, variation, implicitation, pure borrowing, reduction, addition, addaptation, and paraphrase in which established equivalent is most frequently used. With using those techniques, the study indicates that translation quality is very good. They are 2.8 of accuracy and 2.9 of acceptability and readablity. Imperfection of translation quality is affected from the use of reduction and addition in relation to accuracy and the use of deletion in relation to those aspects of translation quality. </p>
Identification of small ruminant external parasite species in Tanqua Abergelle and Kola Tembien districts of Tigray region, Northern Ethiopia
Authors: Assefa Guash Abay 
Published: September 2019

Abstract
Background Ecto-parasites are a major concern in sheep and goat flocks, wherever sheep and goat are kept. A study was carried out in the districts of Kola Tembien and Tanqua Abergelle from September 2014 to June 2017 with the objectives of identifying external parasites to their species level.Methods A cross-sectional study design was conducted. Randomly selected sheep and goat was clinically examined for the presence of ecto-parasites or lesions and identified in National animal health diagnostic and investigation center (NAHDIC) by using taxonomical and entomological examination. About 237 live sheep and goats have been examined randomly for the presence of external parasites.Results All identified species of external parasites were found in both species. Accordingly, eight species of external parasites have been identified with their prevalence as follows; Amblyoma varigatum (35.4%), Ripicephulus parvus (22.8%), Ripicephulus praetexatus (8.4%), Ripicephulus pulchellus (7.6%), Ctenocephalides felis felis (5.1%), Linognatus africanus (11.8%), Ripicephalus evertsi (2.5%), Hayaloma trancatum (6.5%). Both in sheep and goats, there was no significant difference between infestation of external parasites and the associated risk factors except for Amblyoma varigatum which was higher in Tanqua Abergelle (25.7%) than Kola Tembien (9.7%). The odd of infestation by Amblyoma varigatum in goat was 3.5 higher than in sheep.Conclusions Our study revealed that eight species of external parasites has been found with a higher rate of infestation. Therefore, to minimize the prevalence of external parasites urgent and well coordinated external parasites control measures should be taken.
THR alpha and its subtypes alpha1 and alpha2 are prognostic markers for survival of ovarian cancer patients
Authors: Ditsch Nina , Heublein Sabine , Jeschke Udo , Sattler Cornelia , Kuhn Christina , Hester Anna , Czogalla Bastian , Trillsch Fabian , Mahner Sven , Engel Jutta , Mayr Doris , Schmoeckel Elisa 
Published: September 2019

Abstract
Background

Ovarian cancer is most lethal in comparison to all other gynecological cancer cases. Within this study, we aimed to investigate the expression of the thyroid hormone receptor alpha and its influence on patient survival in ovarian cancer (OvCa).

Methods

The presence of the thyroid hormone receptors THRα, THRα1 and -2 were investigated in 156 ovarian cancer samples using immunohistochemistry (IHC) using semi-quantitative immunoreactivity (IR) scores and correlated to clinical, pathological data, subtype of ovarian cancer, clinical data, staining of 20 already described OvCa marker proteins and overall survival (OS).

Results

Patients with clear cell OvCa showed the highest THRα expression and nuclear THRα expression is associated with reduced survival in this group. In contrast, nuclear expressed THRα1 is a positive prognosticator for all OvCa patients. Nuclear THRα2 is a positive prognosticator for OvCa patients of the serous subtype. Cytoplasmic expression of THRα2 accompanies with reduced OS in all OvCa patients, whereas cytoplasmic expression of THRα1 is associated with reduced OS in mucinous OvCa patients only. In addition, THRα expression correlates to gonadotropin receptors, steroid hormone receptors, TA-MUC1 and glycodelin.

Conclusion

Thyroid hormones promote ovarian cancer cell proliferation. The further investigation of thyroid hormones and its specific receptors offer the possibility to investigate new endocrine targets against ovarian cancer.
Network properties of cancer prognostic genes and their gene sets in the human protein interactome
Authors: Zhang Jifeng , Jiang Cheng , Ji Zhicheng , Wang Chenrun 
Published: September 2019

Abstract
Background Identifying prognostic genes (PG) is crucial for estimating survival time and providing pinpoint treatments for patients with cancer. However, prognostic genes sets (PGS) reported in most existing research have low reproducibility and overlap ever between the same cancers or their subtypes. Their common characteristic as well as the molecular mechanism of action is still elusive. Methods Here, we obtained nine prognostic gene sets (including 1,439 prognostic genes) of different types of cancer from 23 high quality literatures, and systemically investigated eight network topological properties for PG and PGS compared with background and four other gene sets (cancer gene set CA, essential gene set ES, housekeeping gene set HK, and metastasis-angiogenesis gene set MA) based on the HPRD and String networks. Results The results showed that PG did not occupy key positions in the human protein interactome network, and were more similar to ES rather than CA. Also, PGS had significantly small intraset distance (IAD) and interset distance (IED) in comparison with random sets. Further, we also found that PGS tended to have be distributed within network modules rather than between modules, the functional intersection of the modules enriched with PGS was closely related to cancer. Conclusions Our research reveals the common properties of cancer PG and PGS in the human protein interactome network, and can help us understand and discover cancer prognostic biomarkers.
Borders on the old maps of Jizera Mountain
Authors: Böhm Hynek , Šmída Jiří 
Published: September 2019

Abstract
Old maps, mainly from the period between 1890 and 1940, have been collected in the framework of the project “Old Maps of the Jizera Mountains”. These maps provide us with a complex picture, mainly of tourism, in this currently Czech–Polish territory.
The territory of the Jizera Mountains was inhabited mainly by a German-speaking population on both sides of the border until 1945. Yet it is interesting to examine how the border between the two states – in those times Czechoslovakia and Germany, now Czechia and Poland – was illustrated on these old maps. This article argues that the border was not perceived as a barrier as such until later on, mainly due to the ethnic change in the borderlands. It also reveals that the borders on the maps are of manifold nature and cannot be simply limited to the national borders.
THE IMPACT OF CIGARETTE ADVERTISEMENTS ON ADOLESCENTS : A LITERATURE REVIEW
Authors: Nurcahyani Eti Wiyati , Kresyca Fera , Indriyani Nadila Sopya , Nurcandra Fajaria 
Published: September 2019

Cigarette consumption occurs throughout the world and is widespread among teenagers. Moreover, the existence of cigarette advertisements as a promotional media that is easily found in various mass media, can provide smoking influences to adolescents. The purpose of this study was to determine the effects of cigarette advertising on adolescent smoking initiation. This study uses a literature review. Google Scholar, Sciene Direct, and Proquest are used to select literature related to the topic to be discussed. The analysis in this study was also assisted with several articles found on the internet. Based on research, the influence of various types of cigarette advertisements in general in various countries can lead to smoking initiation among adolescents. The image in cigarette advertisements makes smokers look attractive. Teenagers who are exposed to cigarette advertisements are more open and have a high curiosity about the use of cigarettes. Some actions need to be taken to reduce cigarette consumption in adolescents, such as providing cigarette education to adolescents, as well as enforcement of strong anti-smoking advertising, promotion and sponsorship policies. So, the teenagers know and understand the harmful effects of using these cigarettes.
Keywords: Cigarette advertisement, adolescent health, smoking.
UJI KELIMPAHAN BAKTERI COLIFORM PADA MAKANAN JAJANAN BAKSO TUSUK YANG DIJUAL DI LINGKUNGAN SDN 82 KUDAMATI DAN SDN 2 TANAH TINGGI AMBON
Authors: Tahya Aknes , Kaihena M , Watuguly Theopilus 
Published: September 2019

Background: Snack food is part of food that is inseparable from the community. Snack food consists of various types, one of which is skewer meatballs. Skewers are a type of snack food made from flour and meat that is round and then boiled until cooked, has a savory and chewy taste. One of the bacteria that often contaminates snacks is coliform bacteria. Coliform bacteria is a group of rod-shaped gram negative bacteria.
Method: The sample used in the study was skewered meatball samples taken at the location of SDN 82 Kudamati and SDN 2 Tanah Tinggi Ambon. Testing for the presence of coliform bacteria was carried out at the Indonesian Institute of Research and Standardization Laboratory in Ambon. The type of research used is descriptive. Calculation of the number of bacterial colonies using the standard Total Plate Count (TPC).
Results: The study showed that the two positive samples contained coliform bacteria with the highest number of coliform bacteria colonies in code A1 1.55 x 105 at location A SDN 82 Kudamati and the lowest at code B1 1.49 x 103 at location B SDN 2 Tanah Tinggi. If compared with SNI for processed meat and chicken products is 1 x 105 colonies / gram, which means the highest number of colonies cannot be consumed.
Conclusion: Skewers meatball snacks sold in SDN 82 Kudamati and Ambon Tanah 2 Elementary School 2, positive for coliform bacteria
PERBEDAAN HASIL BELAJAR SISWA KELAS VII SMP NEGERI 9 AMBON MENGGUNAKAN MODEL PEMBELAJARAN LAPS-HEURISTIC DAN MODEL PEMBELAJARAN MEANDS-ENDS ANALYSIS DIPADUKAN DENGAN MEDIA MOVIE KONSEP EKOSISTEM
Authors: Jacob Frensca A , Tuaputty H 
Published: September 2019

Background: To achieve learning objectives, teachers are required to be able to choose and integrate learning models and learning media that are suitable for application in a particular concept. Although the development of the learning model has undergone a change, in reality teachers still have difficulty in determining the learning model that is suitable to be applied at an educational level.
Method: The study was conducted on 18 September - 18 October 2018 using descriptive methods, student learning outcomes were seen through the final grades assessed based on cognitive (LKS), affective and psychomotor aspects and pre-post test scores. Measurement data were analyzed using t test formula.
Results: Based on the t test, the results of tcount 14.066 and ttable 1.658, tcount> ttable, then the null hypothesis (HO) is rejected and the alternative hypothesis (Ha) is accepted. This shows that there are differences in learning outcomes of class VII students of SMP Negeri 9 Ambon using Laps-Heuristic learning models and Meands-Ends Analysis combined with Movie media.
Conclusion: There are differences in student learning outcomes by using Laps-Heuristic and Meands-Ends Analysis learning models for the ecosystem concept in class VII Ambon 9 State Junior High School. Meands-Ends Analysis learning is more effective for improving student learning outcomes than Laps-Heuristic learning models.