bluuebunny/crossref_metadata_2025_split huggingface dataset:
Latent change‐on‐change between amyloid accumulation and cognitive decline
Authors: Klinger Hannah M. , Healy Brian C. , Hanseeuw Bernard J. , Jones Rich N. , Boyle Rory , Townsend Diana L. , Properzi Michael J. , Coughlan Gillian T. , Seto Mabel , Birkenbihl Colin , Farrell Michelle E. , Papp Kathryn V. , Chhatwal Jasmeer P. , Yang Hyun‐Sik , Schultz Aaron P. , Amariglio Rebecca E. , Jacobs Heidi I. L. , Price Julie C. , Johnson Keith A. , Rentz Dorene M. , Sperling Reisa A. , Buckley Rachel F. 
Published: October 2024

AbstractINTRODUCTIONWhile the influence of cross‐sectional β‐amyloid (Aβ) on longitudinal changes in cognition is well established, longitudinal change‐on‐change between Aβ and cognition is less explored.METHODSA series of bivariate latent change score models (LCSM) examined the relationship between changes in 11C‐Pittsburgh Compound‐B (PiB) positron emission tomography (PET) and the Preclinical Alzheimer's Cognitive Composite‐5 (PACC‐5) while adjusting for covariates, including cross‐sectional medial temporal lobe (MTL) tau‐PET burden. We selected 352 clinically normal older participants with up to 9 years of PiB‐PET and PACC‐5 data from the Harvard Aging Brain Study (HABS).RESULTSAβ accumulation was associated with subsequent cognitive decline beyond the effects of cross‐sectional Aβ burden. Within this model including covariates such as age, sex, and apolipoprotein ε4 (APOEε4) status, we found no evidence supporting previously published associations between cross‐sectional tau‐PET and cognitive intercept/slope.DISCUSSIONShort‐term Aβ changes are significantly associated with cognitive decline in clinically normal older adults and may eclipse the effect of cross‐sectional Aβ and MTL tau.Highlights
Aβ accumulation is associated with subsequent cognitive decline.
High Aβ burden is not the sole metric signaling impending cognitive decline.
Contrary to prior work, MTL tau‐PET and cognition were not associated in our models.
Models of bivariate latent Aβ and cognitive change may eclipse the effects of MTL tau.
Metabolic alterations in human pulmonary artery smooth muscle cells treated with PDGF‐BB
Authors: Zhang Meng‐Jie , Kou Jie‐Jian , Zhang Hong‐Da , Xie Xin‐Mei , Zhou Yun‐Feng , Yuan Ping , Pang Xiao‐Bin , Zhao Lu‐Ling , Qiu Jing , He Yang‐Yang 
Published: October 2024

AbstractBackgroundMetabolic abnormalities are considered to play a key regulatory role in vascular remodeling of pulmonary arterial hypertension. However, to date, there is a paucity of research documenting the changes in metabolome profiles within the supernatants of pulmonary artery smooth muscle cells (PASMC) during their transition from a contractile to a synthetic phenotype.MethodsCCK‐8 and Edu staining assays were used to evaluate the cell viability and proliferation of human PASMCs. IncuCyte ZOOM imaging system was used to continuously and automatically detect the migration of the PASMCs. A targeted metabolomics profiling was performed to quantitatively analyze 121 metabolites in the supernatant. Orthogonal partial least squares discriminant analysis was used to discriminate between PDGF‐BB‐induced PASMCs and controls. Metabolite set enrichment analysis was adapted to exploit the most disturbed metabolic pathways.ResultsHuman PASMCs exhibited a transformation from contractile phenotype to synthetic phenotype after PDGF‐BB induction, along with a significant increase in cell viability, proliferation, and migration. Metabolites in the supernatants of PASMCs treated with or without PDGF‐BB were well profiled. Eleven metabolites were found to be significantly upregulated, whereas seven metabolites were downregulated in the supernatants of PASMCs induced by PDGF‐BB compared to the vehicle‐treated cells. Fourteen pathways were involved, and pyruvate metabolism pathway was ranked first with the highest enrichment impact followed by glycolysis/gluconeogenesis and pyrimidine metabolism.ConclusionsSignificant and extensive metabolic abnormalities occurred during the phenotypic transformation of PASMCs. Disturbance of pyruvate metabolism pathway might contribute to pulmonary vascular remodeling.
A Zn‐MOF With Its Mixed‐Matrix Membranes for Convenient and Efficient Visualization Detection of 3‐Nitrotyrosine Biomarker in Aqueous Media and Practical Application in Serum
Authors: Ru Jing , Shi Yi‐Xuan , Yang Qing‐Yun , Li Teng , Shi Yang , Wang Hai‐Ying , Guo Qiang 
Published: October 2024

ABSTRACTInstant and accurate detection of 3‐nitrotyrosine (3‐NT) biomarker is an important but challenging task in clinical diagnosis, treatment, and monitoring of diseases. Herein, this work reported a Zn (II)–based metal–organic framework (MOF) {[Zn2(BDPT)(DIMB)]}n, denoted as Zn‐BDPT, synthesized by solvothermal method using bis(3,5‐dicarboxyphenyl)terephthalamide (H4BDPT) and 1,4‐di(1H‐imidazol‐1‐yl)butane (DIMB). Neighboring [Zn (COO)2] clusters were connected by BDPT4− units and DIMB to form a (2,4,4)‐connected three‐dimensional (3D) architecture. X‐ray powder diffraction (PXRD) and thermogravimetric (TGA) experiments indicated that Zn‐BDPT had good purity and stability. Zn‐BDPT could selectively and rapidly recognize 3‐NT in an aqueous environment. For rapid economic detection of 3‐NT in actual samples, mixed‐matrix membranes (MMMs) based on Zn‐BDPT were successfully prepared and named Zn‐BDPT@PMMA. The addition of 3‐NT under the UV lamp displayed an evident visible fluorescence quenching effect. Quantitative titration analysis of Zn‐BDPT@PMMA in real serum sample obtained the Ksv value of 1.13 × 104 M−1 and a limit of detection (LOD) of 0.17 μM. This report demonstrated that MOF‐based MMMs could be used as ideal candidate for luminescent probe in actual application.
Phase Regulation Promotes High Rate‐Long Term Na4Fe3(PO4)2P2O7 Cathode for Sodium‐Ion Batteries
Authors: Yang Haiyan , Li Xinhai , Wang Zhixing , Guo Huajun , Duan Hui , Wang Jiexi , Li Guangchao , Yan Guochun 
Published: October 2024

AbstractSodium‐ion batteries (SIBs) have evoked much attention, benefiting from the advantages of low cost, high safety and excellent performance at low temperature. Especially, Na4Fe3(PO4)2P2O7 (NFPP) cathode is considered to be one of the best candidates for SIBs cathode with abundant resources and long‐term cycling stability. However, the impurities of NaFePO4 (NFP) and Na2FeP2O7 (NFPO) formed synchronously with NFPP which restrict the further application of NFPP. It is meaningful to clear the formation process and regulate the contents of NFP and NFPO. Therefore, NFPP cathodes with different contents of NFP and NFPO were prepared through high energy ball milling cooperated with post‐heat treatment by controlling the Fe concentration in reactants. The NFPP‐2.85 showed the best electrochemical performance because of the high content of NFPP and transition zone between NFPP and NFPO which fasts the Na+ transport kinetics. When employed as cathode for SIBs, the as‐prepared NFPP‐2.85 showed a specific capacity of 111.8 mAh g−1 at 0.1 C and maintained at 68.9 mAh g−1 even at 100 C. The retention ratio was as high as 93.6 % after 1500 cycles at 20 C, implying superior high rate‐long term cycling stability. This work provides a new way for impurities regulation and the improvement of NFPP electrochemical performance.
Group Integrative Dynamic Factor Models With Application to Multiple Subject Brain Connectivity
Authors: Kim Younghoon , Fisher Zachary F. , Pipiras Vladas 
Published: October 2024

ABSTRACTThis work introduces a novel framework for dynamic factor model‐based group‐level analysis of multiple subjects time‐series data, called GRoup Integrative DYnamic factor (GRIDY) models. The framework identifies and characterizes intersubject similarities and differences between two predetermined groups by considering a combination of group spatial information and individual temporal dynamics. Furthermore, it enables the identification of intrasubject similarities and differences over time by employing different model configurations for each subject. Methodologically, the framework combines a novel principal angle‐based rank selection algorithm and a noniterative integrative analysis framework. Inspired by simultaneous component analysis, this approach also reconstructs identifiable latent factor series with flexible covariance structures. The performance of the GRIDY models is evaluated through simulations conducted under various scenarios. An application is also presented to compare resting‐state functional MRI data collected from multiple subjects in autism spectrum disorder and control groups.
High‐Dimensional Bayesian Semiparametric Models for Small Samples: A Principled Approach to the Analysis of Cytokine Expression Data
Authors: Poli Giovanni , Argiento Raffaele , Amedei Amedeo , Stingo Francesco C. 
Published: October 2024

ABSTRACTIn laboratory medicine, due to the lack of sample availability and resources, measurements of many quantities of interest are commonly collected over a few samples, making statistical inference particularly challenging. In this context, several hypotheses can be tested, and studies are not often powered accordingly. We present a semiparametric Bayesian approach to effectively test multiple hypotheses applied to an experiment that aims to identify cytokines involved in Crohn's disease (CD) infection that may be ongoing in multiple tissues. We assume that the positive correlation commonly observed between cytokines is caused by latent groups of effects, which in turn result from a common cause. These clusters are effectively modeled through a Dirichlet Process (DP) that is one of the most popular choices as nonparametric prior in Bayesian statistics and has been proven to be a powerful tool for model‐based clustering. We use a spike–slab distribution as the base measure of the DP. The nonparametric part has been included in an additive model whose parametric component is a Bayesian hierarchical model. We include simulations that empirically demonstrate the effectiveness of the proposed testing procedure in settings that mimic our application's sample size and data structure. Our CD data analysis shows strong evidence of a cytokine gradient in the external intestinal tissue.
Protein Scaffold‐Mediated Multi‐Enzyme Self‐Assembly and Ordered Co‐Immobilization of Flavin‐Dependent Halogenase‐Coenzyme Cycle System for Efficient Biosynthesis of 6‐Cl‐L‐Trp
Authors: Liu Han‐Yu , Ning Pan , Qian Feng , Wang Yao‐Wu , Zhang Hai‐Min , Wang Pu 
Published: October 2024

ABSTRACTFlavin‐dependent halogenase (FDH) is highly prized in pharmaceutical and chemical industries for its exceptional capacity to produce halogenated aromatic compounds with precise regioselectivity. This study has devised a multi‐enzyme self‐assembly strategy to construct an effective and reliable in vitro coenzyme cycling system tailored for FDHs. Initially, tri‐enzyme self‐assembling nanoclusters (TESNCs) were developed, comprising glucose dehydrogenase (GDH), flavin reductase (FR) and FDH. The TESNCs exhibited enhanced thermal stability and conversion efficiency compared to free triple enzyme mixtures during the conversion of L‐Trp to 6‐Cl‐L‐Trp, resulting in a 2.1‐fold increase in yield. Subsequently, an ordered co‐immobilization of GDH, FR, and FDH was established, further amplifying the stability and catalytic efficiency of the FDH coenzyme cycle system. Compared to the free TESNCs, the immobilized TESNCs demonstrated a 4.2‐fold increase in catalytic efficiency in a 5 mL reaction system. This research provides an effective strategy for developing a robust and efficient coenzyme recycling system for FDHs.
Online Eye Tracking for Aphasia: A Feasibility Study Comparing Web and Lab Tracking and Implications for Clinical Use
Authors: van Boxtel Willem S. , Linge Michael , Manning Rylee , Haven Lily N. , Lee Jiyeon 
Published: October 2024

ABSTRACTBackground & AimsStudies using eye‐tracking methodology have made important contributions to the study of language disorders such as aphasia. Nevertheless, in clinical groups especially, eye‐tracking studies often include small sample sizes, limiting the generalizability of reported findings. Online, webcam‐based tracking offers a potential solution to this issue, but web‐based tracking has not been compared with in‐lab tracking in past studies and has never been attempted in groups with language impairments.Materials & MethodsPatients with post‐stroke aphasia (n = 16) and age‐matched controls (n = 16) completed identical sentence‐picture matching tasks in the lab (using an EyeLink system) and on the web (using WebGazer.js), with the order of sessions counterbalanced. We examined whether web‐based eye tracking is as sensitive as in‐lab eye tracking in detecting group differences in sentence processing.ResultsPatients were less accurate and slower to respond to all sentence types than controls. Proportions of gazes to the target and foil picture were computed in 100 ms increments, which showed that the two modes of tracking were comparably sensitive to overall group differences across different sentence types. Web tracking showed comparable fluctuations in gaze proportions to target pictures to lab tracking in most analyses, whereas a delay of approximately 500–800 ms appeared in web compared to lab data.Discussion & ConclusionsWeb‐based eye tracking is feasible to study impaired language processing in aphasia and is sensitive enough to detect most group differences between controls and patients. Given that validations of webcam‐based tracking are in their infancy and how transformative this method could be to several disciplines, much more testing is warranted.
Biological and Biophysical Methods for Evaluation of Inhibitors of Sortase A in Staphylococcus aureus: An Overview
Authors: Dewan Debajit , Basu Aaheli , Dolai Debraj , Pal Sarmistha 
Published: October 2024

ABSTRACTStaphylococcus aureus, one of the most notorious pathogens, develops antibiotic resistance by the formation of a thick layer of exopolysaccharides known as biofilms. Sortase A, a transpeptidase responsible for biofilm formation and attachment to the host surface, has emerged as an important drug target for development of anti‐virulence agents. A number of sortase A inhibitors, both peptide and non‐peptides are reported which involved the use of several experiments which may provide insights regarding binding affinity, specificity, safety, and efficacy of ligands. In this review, we focus on the principles, pros and cons, and the type of information obtained from biophysical (FRET assay, Microscale Thermophoresis, Surface Plasmon Resonance, CD spectroscopy etc.) and biological (cell viability assay, biofilm formation assay, CLSM, western blot analysis, in vivo characterization on mice etc.) methods for estimation of probable sortase A inhibitors, which might be helpful to the researchers who might be interested to delve into the development of sortase A inhibitors as a drug, to address the burning question of antimicrobial resistance (AMR).
Marker‐Dependent Cleavage of RNA by Binary (split) DNAzyme (BiDz) and Binary DNA Machines (BiDM)
Authors: Dubovichenko Mikhail V. , Nedorezova Daria D. , Patra Christina , Drozd Valeria S. , Andrianov Vladimir S. , Ashmarova Anna I. , Nnanyereugo Vivian O. , Eldeeb Ahmed A. , Kolpashchikov Dmitry M. 
Published: October 2024

AbstractOligonucleotide gene therapy (OGT) can be used to suppress specific RNA in cells and thus has been explored for gene therapy. Despite extensive effort, there is no clinically significant OGT for treating cancer. Low efficiency of OGT is one of the problems. Earlier, we proposed to address this problem by suppressing the most vital genes in cancer cells e. g. housekeeping genes. To achieve specific activation of the OGT agents in cancer but not in normal cells, we designed a binary (split) DNAzyme (BiDz) activated by cancer‐related nucleic acid sequences. This work is devoted to BiDz optimization for efficient cleavage of structured RNA targets upon activation with a cancer marker‐related sequence. To achieve efficient binding of folded RNA, the BiDz was equipped with RNA binding/unwinding arms to produce ‘Binary DNA‐nanomachines’ (BiDM). It was demonstrated that BiDM can improve both the rate and selectivity of RNA cleavage in comparison with BiDz. For the best selectivity, single‐nucleotide variations should be recognized by the strand detached from the common DNA scaffold of BiDM. Further development of DNA nanotechnology‐inspired agents can advance OGT in treating cancer, viral infections, and genetic disorders.
Intravascular Ultrasound Assessment of Distal Trans‐Radial Access in Patients Undergoing Percutaneous Coronary Intervention
Authors: Poletti Enrico , Colletti Giuseppe , Zivelonghi Carlo , Benedetti Alice , Moroni Alice , El Jattari Hicham , Natalis Alexandre , Rubino Francesca , Scott Benjamin , Bentakhou Elias , Haine Steven E. F. , Agostoni Pierfrancesco , Ungureanu Claudiu 
Published: October 2024

ABSTRACTBackgroundDistal trans‐radial access (dTRA) for percutaneous coronary interventions (PCI) is increasingly gaining attention due to its potential to mitigate radial artery occlusion (RAO). However, a comprehensive understanding of the mechanical impact of the devices on the radial artery (RA) wall remains limited. Using a complete intravascular ultrasound (IVUS) evaluation of the RA, including also the vascular access site, we aimed to evaluate all the consequences related to the catheterization on the RA wall, starting from the vascular access, comparing conventional sheath and sheathless approaches.MethodsThis is an observational, prospective, multicenter study aimed to assess the entire RA wall immediately after IVUS‐guided PCI via‐dTRA. IVUS assessment included quantitative measurements (minimal lumen area [MLA], minimal vessel area [MVA]) and qualitative observations (dissections, vasospasm). Study objectives included delineating RA wall structure post‐PCI and comparing findings between conventional and sheathless approaches.ResultsFifty patients (21 [42%] with conventional sheath, 29 [58%] sheathless) were enrolled between March 2023 and February 2024. Female patients were more prevalent in the convention sheath group (38% vs. 7%, p < 0.001). Sheathless approach utilized 7‐French guiding catheters more frequently (33% vs. 86%, p < 0.001). Post‐procedural IVUS identified dissections in 12% of cases, with no significant difference between approaches. Arterial vasospasm was present in a quarter of patients, numerically higher in the conventional sheath group (29% vs. 21%, p = 0.5). MLA and MVA were comparable between groups, though MLA and MVA were lowest at the proximal segment of the RA only in the conventional sheath group (p < 0.001). No RAO was documented during the IVUS evaluation.ConclusionsThe intravascular assessment of dTRA after coronary interventions, utilizing either conventional or sheathless approaches, including large‐bore guiding catheters, demonstrated a relatively low incidence of access‐related complications such as dissection and vasospasm, without affecting the flow and patency of the proximal RA.
Anion‐substituted Hybrid Zinc Halides with Remarkable Dielectric Switches
Authors: Zhou Lin , Lu Zheng Lan , Mo Wen Feng , Yu Yu Hui , Zhai Li Xia , Wei Wen Juan , Yao Huilu 
Published: October 2024

AbstractThermo‐responsive dielectric switching hybrid halides, displaying reversible dielectric bistability, have recently made them highly versatile and attractive for their structural tunability and rich functional properties. However, the substantial improvement of the dielectric switching operating range is limited near room temperature, and obtaining ultra‐wide dielectric switching temperature region is a major challenge. Herein, a remarkable dielectric switching effect is reported with ultra‐wide dielectric switching temperature region in a hybrid halide system, caused by dipolar orientational polarization ascribing order‐disorder phase transitions. The new A2BX4 hybrid halide [C6H5(CH2)4NH3]2ZnBr4 (4PBA‐ZnBr4) with an ultra‐wide dielectric hysteresis loop of about 55 K near room temperature is designed successfully through halogen substitution. Meanwhile, structurally similar hybrid materials 4PBA‐ZnI4 were designed as controls. More interestingly, the crystal structure, phase transition temperature (Tc), dielectric and semiconductor properties exhibit significant discrepancies as the halogen atoms vary from Br to I. This discovery provides an efficiently regulated anionic framework to construct hybrid halides with ultra‐wide high dielectric switching and reliable cycling stability by assembling with various cations, making them potentially excellent candidates for highly responsive room temperature smart switches or transducer devices.
Triarylmethanol Derivatives with Ultralong Organic Room‐Temperature Phosphorescence
Authors: Li Jinwei , Duan Ruikang , Zhu Yanmei , Chen Mulin , Dai Xianyin , Duan Guiyun , Ge Yanqing 
Published: October 2024

AbstractTriphenylmethyl‐based compounds such as rhodamines and fluoresceine representing an old and well‐known class of triphenylmethane dyes, are widely used in fluorescent labeling of bioimaging. Inspired by ultralong room temperature phosphorescence of triphenylphosphine derivatives, herein we report a methoxy substituted triarylmethanol ((4‐methoxyphenyl)diphenylmethanol, LJW‐1) exhibits ultralong room temperature phosphorescence (RTP) under ambient condition with afterglows of about 7 seconds. Its multiple C−H ⋅ ⋅ ⋅ π intermolecular interactions, C−H ⋅ ⋅ ⋅ O intermolecular interactions, hydrogen bond and π‐π interactions are beneficial for forming rigid environment in the aggregated state which is evidently an important factor in the appearance of excellent RTP. Different substituents on triarylmethanol result in compounds with different lifetimes varying from 7 μs to 818 ms. The substituent effects on the phosphorescence of triarylmethanol derivatives provide an efficient method for the design of organic RTP materials and may be enlightening the phosphorescence research of triarylmethanol derivatives.
Trade‐offs between defense and competitive traits in a planktonic predator–prey system
Authors: Réveillon Tom , Becks Lutz 
Published: October 2024

AbstractPredator–prey interactions are crucial components of populations and communities. Their dynamics depend on the covariation of traits of the interacting organisms, and there is increasing evidence that intraspecific trade‐off relationships between defense and competitive traits are important drivers of trophic interactions. However, quantifying the relevant traits forming defense–competitiveness trade‐offs and how these traits determine prey and predator fitness remains a major challenge. Here, we conducted feeding and growth experiments to assess multiple traits related to defense and competitiveness in six different strains of the green alga Chlamydomonas reinhardtii exposed to predation by the rotifer Brachionus calyciflorus. We found large differences in defense and competitive traits among prey strains and negative relationships between these traits for multiple trait combinations. Because we compared trait differences among strains whose ancestors evolved previously in controlled environments where selection favored either defense or competitiveness, these negative correlations suggest the presence of a trade‐off between defense and competitiveness. These differences in traits and trade‐offs translated into differences in prey and predator fitness, demonstrating the importance of intraspecific trade‐offs in predicting the outcome of predator–prey interactions.