bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mycobacterium tuberculosis: Bacterial Fitness within the Host Macrophage
Authors: Huang Lu , Nazarova Evgeniya V. , Russell David G. 
Published: March 2019

ABSTRACTMycobacterium tuberculosishas evolved to become the single greatest cause of death from an infectious agent. The pathogen spends most of its infection cycle in its human host within a phagocyte. The bacterium has evolved to block the normal maturation and acidification of its phagosome and resides in a vacuole contiguous with the early endosomal network. Cytokine-mediated activation of the host cell can overcome this blockage, and an array of antimicrobial responses can limit its survival. The survival ofM. tuberculosisin its host cell is fueled predominantly by fatty acids and cholesterol. The ability ofM. tuberculosisto degrade sterols is an unusual metabolic characteristic that was likely retained from a saprophytic ancestor. Recent results with fluorescentM. tuberculosisreporter strains demonstrate that bacterial survival differs with the host macrophage population. Tissue-resident alveolar macrophages, which are biased towards an alternatively activated, M2-like phenotype, are more permissive to bacterial growth than monocyte-derived, inflammatory, M1-like interstitial macrophages. The differential growth of the bacterium in these different phagocyte populations appears to be linked to host cell metabolism.
The Intracellular Life Cycle of
Brucella
spp
Authors: Celli Jean 
Published: March 2019

ABSTRACT

Bacteria of the genus
Brucella
colonize a wide variety of mammalian hosts, in which their infectious cycle and ability to cause disease predominantly rely on an intracellular lifestyle within phagocytes. Upon entry into host cells,
Brucella
organisms undergo a complex, multistage intracellular cycle in which they sequentially traffic through, and exploit functions of, the endocytic, secretory, and autophagic compartments via type IV secretion system (T4SS)-mediated delivery of bacterial effectors. These effectors modulate an array of host functions and machineries to first promote conversion of the initial endosome-like
Brucella
-containing vacuole (eBCV) into a replication-permissive organelle derived from the host endoplasmic reticulum (rBCV) and then to an autophagy-related vacuole (aBCV) that mediates bacterial egress. Here we detail and discuss our current knowledge of cellular and molecular events of the
Brucella
intracellular cycle. We discuss the importance of the endosomal stage in determining T4SS competency, the roles of autophagy in rBCV biogenesis and aBCV formation, and T4SS-driven mechanisms of modulation of host secretory traffic in rBCV biogenesis and bacterial egress.
The Many Faces of Bacterium-Endothelium Interactions during Systemic Infections
Authors: Obino Dorian , Duménil Guillaume 
Published: March 2019

ABSTRACT
A wide variety of pathogens reach the circulatory system during viral, parasitic, fungal, and bacterial infections, causing clinically diverse pathologies. Such systemic infections are usually severe and frequently life-threatening despite intensive care, in particular during the age of antibiotic resistance. Because of its position at the interface between the blood and the rest of the organism, the endothelium plays a central role during these infections. Using several examples of systemic infections, we explore the diversity of interactions between pathogens and the endothelium. These examples reveal that bacterial pathogens target specific vascular beds and affect most aspects of endothelial cell biology, ranging from cellular junction stability to endothelial cell proliferation and inflammation.
New Age Strategies To Reconstruct Mucosal Tissue Colonization and Growth in Cell Culture Systems
Authors: Fasciano Alyssa C. , Mecsas Joan , Isberg Ralph R. 
Published: March 2019

ABSTRACT

Over the past few decades,
in vitro
cell culture systems have greatly expanded our understanding of host-pathogen interactions. However, studies using these models have been limited by the fact that they lack the complexity of the human body. Therefore, recent efforts that allow tissue architecture to be mimicked during
in vitro
culture have included the development of methods and technology that incorporate tissue structure, cellular composition, and efficient long-term culture. These advances have opened the door for the study of pathogens that previously could not be cultured and for the study of pathophysiological properties of infection that could not be easily elucidated using traditional culture models. Here we discuss the latest studies using organoids and engineering technology that have been developed and applied to the study of host-pathogen interactions in mucosal tissues.
How and why front-line health workers (did not) use a multifaceted mHealth intervention to support maternal and neonatal healthcare decision-making in Ghana
Authors: Amoakoh Hannah Brown , Klipstein-Grobusch Kerstin , Ansah Evelyn Korkor , Grobbee Diederick E , Yveoo Linda , Agyepong Irene 
Published: March 2019

IntroductionDespite increasing use of mHealth interventions, there remains limited documentation of ‘how and why’ they are used and therefore the explanatory mechanisms behind observed effects on beneficiary health outcomes. We explored ‘how and why’ an mHealth intervention to support clinical decision-making by front-line providers of maternal and neonatal healthcare services in a low-resource setting was used. The intervention consisted of phone calls (voice calls), text messaging (short messaging service (SMS)), internet access (data) and access to emergency obstetric and neonatal protocols via an Unstructured Supplementary Service Data (USSD). It was delivered through individual-use and shared facility mobile phones with unique Subscriber Identification Module (SIM) cards networked in a Closed User Group.MethodsA single case study with multiple embedded subunits of analysis within the context of a cluster randomised controlled trial of the impact of the intervention on neonatal health outcomes in the Eastern Region of Ghana was performed. We quantitatively analysed SIM card activity data for patterns of voice calls, SMS, data and USSD. We conducted key informant interviews and focus group discussions with intervention users and manually analysed the data for themes.ResultsOverall, the phones were predominantly used for voice calls (64%), followed by data (28%), SMS (5%) and USSD (2%), respectively. Over time, use of all intervention components declined. Qualitative analysis showed that individual health worker factors (demographics, personal and work-related needs, perceived timeliness of intervention, tacit knowledge), organisational factors (resource availability, information flow, availability, phone ownership), technological factors (attrition of phones, network quality) and client perception of health worker intervention usage explain the pattern of intervention use observed.ConclusionHow and why the mHealth intervention was used (or not) went beyond the technology itself and was influenced by individual and context-specific factors. These must be taken into account in designing similar interventions to optimise effectiveness.
Effect of the competency-basedHelping Mothers Survive Bleeding after Birth(HMS BAB) training on maternal morbidity: a cluster-randomised trial in 20 districts in Tanzania
Authors: Alwy Al-beity Fadhlun , Pembe Andrea , Hirose Atsumi , Morris Jessica , Leshabari Sebalda , Marrone Gaetano , Hanson Claudia 
Published: March 2019

BackgroundTraining health providers is an important strategy to improve health. We conducted a cluster-randomised two-arm trial in Tanzania to assess the effect of a 1-day competency-based training ‘Helping Mothers Survive Bleeding after Birth (HMS BAB)’ followed by eight weekly drills on postpartum haemorrhage (PPH)-related morbidity and mortality.MethodsTwenty districts in four purposefully selected regions in Tanzania included 61 facilities. The districts were randomly allocated using matched pairs to ensure similarity in terms of district health services in intervention and comparison districts. In the 10 intervention districts 331 health providers received the HMS BAB training. The other half continued with standard practices. We used the WHO’s near miss tool to collect information on severe morbidity (near misses) of all women admitted to study facilities. We performed interrupted time series analysis to estimate differences in the change of near miss per delivery rate and case fatality rates. We also assessed implementation of evidence-based preventive and basic management practices for PPH as secondary outcomes.ResultsWe included 120 533 facility deliveries, 6503 near misses and 202 maternal deaths in study districts during study period (November 2014 to January 2017). A significant reduction of PPH near misses was found among women who suffered PPH in the intervention district compared with comparison districts (difference-in-differences of slopes −5.3, 95% CI −7.8 to −2.7, p<0.001) from a baseline PPH-related near miss rate of 71% (95% CI 60% to 80%). There was a significant decrease in the long-term PPH near miss case fatality (difference-in-differences of slopes −4 to 0) (95% CI −6.5 to −1.5, p<0.01) in intervention compared with the comparison districts. The intervention had a positive effect on the proportion of PPH cases treated with intravenous oxytocin (difference-in-differences of slopes 5.2, 95% CI 1.4 to 8.9) (p <0.01).ConclusionThe positive effect of the training intervention on PPH morbidity and case fatality suggests that the training addresses important deficits in knowledge and skills.Trial registration numberPACTR201604001582128.
Prevalence and characteristics of chronic fatigue syndrome/myalgic encephalomyelitis (CFS/ME) in Poland: a cross-sectional study
Authors: Słomko Joanna , Newton Julia L , Kujawski Sławomir , Tafil-Klawe Małgorzata , Klawe Jacek , Staines Donald , Marshall-Gradisnik Sonya , Zalewski Pawel 
Published: March 2019

ObjectivesThe aim of this study was to estimate the prevalence of chronic fatigue syndrome/myalgic encephalomyelitis (CFS/ME) and describe illness characteristics in a community population in Poland. Design: cross-sectional study.SettingPoland.ParticipantsOf the cohort of 1400 who self-presented with fatigue only 69 subsequently were confirmed as having CFS/ME using the Fukuda criteria.Main outcome measuresParticipants completed the following screening symptom assessment tools: Chalder Fatigue Scale, Hospital Anxiety and Depression Scale (HADS), Epworth Sleepiness Scale (ESS), Composite Autonomic Symptom Score 31 (COMPASS 31), Quality of Life Scale (QOLS). Haemodynamic and autonomic parameters were automatically measured at rest with a Task Force Monitor.ResultsIn 1308, from 1400 (93%) individuals who identified themselves as fatigued, recognised chronic conditions were identified, for example, neurological (n=280, 21.5%), neurodegenerative (n=200, 15%), psychiatric (n=654, 50%) and immunologic (n=174, 13.5%) disorders. The remaining 69 participants (mean age 38.3±8.5) met the Fukuda defintion for CFS/ME and had baseline objective assessment. The majority had experienced symptoms for over 2 years with 37% having symptoms for 2–5 years and 21.7% for more than 10 years. The COMPASS 31 indicated that 50% have symptoms consistent with orthostatic intolerance. About 43/69 (62%) had Epworth sleepiness scores ≥10, ie, consistent with excessive daytime sleepiness, 26/69 (38%) had significant anxiety and 22/69 (32%) depression measured by HADS A & D. Quality of life is significantly impaired in those with Fukuda criteria CFS (QLS score 64±11) with significant negative relationships between quality of life and fatigue (p<0.0001), anxiety (p=0.0009), depression (p<0.0001) and autonomic symptoms (p=0.04).ConclusionThis is the first study to summarise illness characteristics of Polish CFS/ME patients. Our study has confirmed that fatigue is a common and under-recognised symptom affecting the Polish population.
Mapping genetic modifiers of radiation-induced cardiotoxicity to rat chromosome 3
Authors: Schlaak Rachel A. , Frei Anne , Schottstaedt Aronne M. , Tsaih Shirng-Wern , Fish Brian L. , Harmann Leanne , Liu Qian , Gasperetti Tracy , Medhora Meetha , North Paula E. , Strande Jennifer L. , Sun Yunguang , Rui Hallgeir , Flister Michael J. , Bergom Carmen 
Published: March 2019

Radiation therapy is used in ~50% of cancer patients to reduce the risk of recurrence and in some cases improve survival. Despite these benefits, doses can be limited by toxicity in multiple organs, including the heart. The underlying causes and biomarkers of radiation-induced cardiotoxicity are currently unknown, prompting the need for experimental models with inherent differences in sensitivity and resistance to the development of radiation-induced cardiotoxicity. We have identified the parental SS (Dahl salt-sensitive/Mcwi) rat strain to be a highly-sensitized model of radiation-induced cardiotoxicity. In comparison, substitution of rat chromosome 3 from the resistant BN (Brown Norway) rat strain onto the SS background (SS-3BN consomic) significantly attenuated radiation-induced cardiotoxicity. SS-3BN rats had less radiation-induced cardiotoxicity than SS rats, as measured by survival, pleural and pericardial effusions, echocardiogram parameters, and histological damage. Mast cells, previously shown to have predominantly protective roles in radiation-induced cardiotoxicity, were increased in the more resistant SS-3BN hearts postradiation. RNA sequencing from SS and SS-3BN hearts at 1 wk postradiation revealed 5,098 differentially expressed candidate genes across the transcriptome and 350 differentially expressed genes on rat chromosome 3, which coincided with enrichment of multiple pathways, including mitochondrial dysfunction, sirtuin signaling, and ubiquitination. Upstream regulators of enriched pathways included the oxidative stress modulating transcription factor, Nrf2, which is located on rat chromosome 3. Nrf2 target genes were also differentially expressed in the SS vs. SS-3BN consomic hearts postradiation. Collectively, these data confirm the existence of heritable modifiers in radiation-induced cardiotoxicity and provide multiple biomarkers, pathways, and candidate genes for future analyses. NEW & NOTEWORTHY This novel study reveals that heritable genetic factors have the potential to modify normal tissue sensitivity to radiation. Gene variant(s) on rat chromosome 3 can contribute to enhanced cardiotoxicity displayed in the SS rats vs. the BN and SS-3BN consomic rats. Identifying genes that lead to understanding the mechanisms of radiation-induced cardiotoxicity represents a novel method to personalize radiation treatment, as well as predict the development of radiation-induced cardiotoxicity.
Interdecadal Seesaw of Precipitation Variability between North China and the Southwest United States
Authors: Yang Qing , Ma Zhuguo , Wu Peili , Klingaman Nicholas P. , Zhang Lixia 
Published: March 2019

AbstractThis paper reports a consistent seesaw relationship between interdecadal precipitation variability over North China and the Southwest United States, which can be found in observations and simulations with several models. Idealized model simulations suggest the seesaw could be mainly driven by the interdecadal Pacific oscillation (IPO), through a large-scale circulation anomaly occupying the entire northern North Pacific, while the Atlantic multidecadal oscillation (AMO) contributes oppositely and less. Modulation of precipitation by the IPO tends to be intensified when the AMO is in the opposite phase, but weakened when the AMO is in the same phase. The warm IPO phase is associated with an anomalous cyclone over the northern North Pacific; consequently, anomalous southwesterly winds bring more moisture and rainfall to the Southwest United States, while northwesterly wind anomalies prevail over North China with negative rainfall anomalies. The east–west seesaw of rainfall anomalies reverses sign when the circulation anomaly becomes anticyclonic during the cold IPO phase. The IPO-related tropical SST anomalies affect the meridional temperature gradient over the North Pacific and adjacent regions and the mean meridional circulation. In the northern North Pacific, the atmospheric response to IPO forcing imposes an equivalent barotropic structure throughout the troposphere. An important implication from this study is the potential predictability of drought-related water stresses over these arid and semiarid regions, with the progress of our understanding and prediction of the IPO and AMO.
Atmospheric Duct Detection Using Wind Profiler Radar and RASS
Authors: Wang Hua , Su Shipeng , Tang Haichuan , Jiao Lin , Li Yunbo 
Published: March 2019

AbstractA method of detecting atmospheric ducts using a wind profiler radar (WPR) and a radio acoustic sounding system (RASS) is proposed. The method uses the RASS to measure the virtual temperature profile and calculate the Brunt–Väisälä frequency; it also uses the WPR to measure the spectral width of the atmosphere and the atmospheric refractive index structure constant. Then the profile of the atmospheric refractive index gradient and modified refractivity are calculated using virtual temperature, spectral width, and the atmospheric refractive index structure constant. Finally, the height and intensity of the atmospheric duct are calculated to achieve continuous monitoring of the atmospheric duct. To verify the height and intensity of the atmospheric duct, comparison experiments between WPR-RASS and radiosondes were carried out from June 2014 to June 2015 in Dalian, Liaoning Province, China. The results show that the profile of modified refractivity by WPR-RASS has exactly the same trend as the radiosondes, the two methods have a good consistency, and the atmospheric duct value from WPR-RASS is in good agreement with that from radiosondes.
Forest-Based and Semiparametric Methods for the Postprocessing of Rainfall Ensemble Forecasting
Authors: Taillardat Maxime , Fougères Anne-Laure , Naveau Philippe , Mestre Olivier 
Published: March 2019

AbstractTo satisfy a wide range of end users, rainfall ensemble forecasts have to be skillful for both low precipitation and extreme events. We introduce local statistical postprocessing methods based on quantile regression forests and gradient forests with a semiparametric extension for heavy-tailed distributions. These hybrid methods make use of the forest-based outputs to fit a parametric distribution that is suitable to model jointly low, medium, and heavy rainfall intensities. Our goal is to improve ensemble quality and value for all rainfall intensities. The proposed methods are applied to daily 51-h forecasts of 6-h accumulated precipitation from 2012 to 2015 over France using the Météo-France ensemble prediction system called Prévision d’Ensemble ARPEGE (PEARP). They are verified with a cross-validation strategy and compete favorably with state-of-the-art methods like analog ensemble or ensemble model output statistics. Our methods do not assume any parametric links between the variables to calibrate and possible covariates. They do not require any variable selection step and can make use of more than 60 predictors available such as summary statistics on the raw ensemble, deterministic forecasts of other parameters of interest, or probabilities of convective rainfall. In addition to improvements in overall performance, hybrid forest-based procedures produced the largest skill improvements for forecasting heavy rainfall events.
Assessment of knowledge, attitudes, and behaviors regarding breast and cervical cancer among women in western Turkey
Authors: Korkut Yasemin 
Published: March 2019

Objective We aimed to assess knowledge, attitudes, and practices regarding breast and cervical cancer and screening methods among women living western Turkey. Methods A questionnaire survey was administered to women aged ≥21 years. Data were collected using a 12-item questionnaire measuring women's knowledge, attitudes, and practice levels, including among participants who were health workers. Results A total 668 women were included in the study. The average age was 37.48 ± 11.85 years. Most women had a primary-level education (43.4%) and most (50.3%) were homemakers; 27.1% of participants were health care workers. The differences in age, education, and occupation among participants were evaluated according to whether participants perform breast self-examination and have undergone Pap testing. The distribution of women according to age group showed that with increased age, the frequency of performing these two behaviors decreased, with women over 55 years old accounting for a significantly higher proportion than other age groups. Conclusions In our study, the level of knowledge and attitudes regarding breast and cervical cancers among women was similar to that in previous studies and was higher than expected, especially among women who were health workers. However, all women had inadequate frequency of performing screening tests.
Predicting major mental illness: ethical and practical considerations
Authors: Lawrie Stephen M. , Fletcher-Watson Sue , Whalley Heather C. , McIntosh Andrew M. 
Published: March 2019

SummaryAn increasing body of genetic and imaging research shows that it is becoming possible to forecast the onset of major psychiatric disorders such as depression and schizophrenia before people become ill with ever improving accuracy. Practical issues such as the optimal combination of clinical and biological variables are being addressed, but the application of predictive algorithms to individuals or in routine clinical settings have yet to be tested. The development of predictive methods in mental health comes with substantial ethical questions, including whether people wish to know their level of risk, as well as individual and societal attitudes to the potential adverse effects of data sharing, early diagnosis and treatment, which so far have been largely ignored. Preliminary data suggests that at least some people think predictive research is valuable and would take part in such studies, and some would welcome knowing the results. Future initiatives should systematically assess opinions and attitudes in conjunction with scientific and technical advances.Declaration of interestIn the past 3 years, S.M.L. has received personal fees from Otsuaka, Sunovion and Janssen, and research grant support from Janssen and Lundbeck. A.M.M. has received research support from the Sackler Trust, Eli Lilly and Janssen. S.M.L. is part of the PSYSCAN consortium.