bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcorneal electrical stimulation restores DNA methylation changes in retinal degeneration
Authors: Tew Ben Yi , Gooden Gerald C. , Lo Pei-An , Pollalis Dimitrios , Ebright Brandon , Kalfa Alex J. , Gonzalez-Calle Alejandra , Thomas Biju , Buckley David N. , Simon Thomas , Yang Zeyi , Iseri Ege , Dunton Cody L. , Backman Vadim , Louie Stan , Lazzi Gianluca , Humayun Mark S. , Salhia Bodour 
Published: December 2024

BackgroundRetinal degeneration is a major cause of irreversible blindness. Stimulation with controlled low-level electrical fields, such as transcorneal electrical stimulation (TES), has recently been postulated as a therapeutic strategy. With promising results, there is a need for detailed molecular characterization of the therapeutic effects of TES.MethodsControlled, non-invasive TES was delivered using a custom contact lens electrode to the retinas of Royal College of Surgeons (RCS) rats, a model of retinal degeneration. DNA methylation in the retina, brain and cell-free DNA in plasma was assessed by reduced representation bisulfite sequencing (RRBS) and gene expression by RNA sequencing.ResultsTES induced DNA methylation and gene expression changes implicated in neuroprotection in the retina of RCS rats. We devised an epigenomic-based retinal health score, derived from DNA methylation changes observed with disease progression in RCS rats, and showed that TES improved the epigenomic health of the retina. TES also induced DNA methylation changes in the superior colliculus: the brain which is involved in integrating visual signaling. Lastly, we demonstrated that TES-induced retinal DNA methylation changes were detectable in cell-free DNA derived from plasma.ConclusionTES induced DNA methylation changes with therapeutic effects, which can be measured in circulation. Based on these changes, we were able to devise a liquid biopsy biomarker for retinal health. These findings shed light on the therapeutic potential and molecular underpinnings of TES, and provide a foundation for the further development of TES to improve the retinal health of patients with degenerative eye diseases.
Validation of GLIM criteria for hospital malnutrition diagnosis by comparison of three different anthropometric approaches to evaluate reduced muscle mass: a prospective cohort study
Authors: Jazinaki Mostafa Shahraki , Safarian Mohammad , Arabi Seyyed Mostafa , Jamali Jamshid , Norouzy Abdolreza 
Published: December 2024

Background and aimThe Global Leadership Initiative on Malnutrition (GLIM) recently proposed a new malnutrition diagnostic tool known as the GLIM criteria. The GLIM criteria need confirmed validation before being widely used in each population or healthcare system. This study aimed to investigate the validation of the GLIM criteria for malnutrition diagnosis in hospitalized patients.MethodsThe content validity was assessed by calculating the content validity ratio (CVR) and content validity index (CVI). Subjective global assessment (SGA) is considered the reference tool to diagnose malnutrition in concurrent validation. In addition, the Kuder–Richardson 20 was used to evaluate the reliability of the GLIM criteria. Furthermore, hospital mortality, length of hospitalization (LOS), prolonged hospital stays (LOS &gt;6 days), 30-day hospital readmission, and 30- and 60-day mortality were identified as malnutrition-related outcomes in predictive validity.ResultsA total of 332 adult/elderly hospitalized patients (median age: 58 (IQR: 24.7), 60.5% men) were enrolled to present the study. Appling GLIM criteria by considering the calf circumference &lt; 31 cm in both genders or mid-upper arm (MUAC) &lt; 23 cm in men and MUAC &lt;22 cm in women as reduced muscle mass had an appropriate accuracy (84.6 and 83.4%, respectively), good ability to distinguish malnourished patients (AUC ROC: 0.85 and 0.83, respectively), satisfactory sensitivity (89.58 and 84.02%, respectively), and satisfactory specificity (81 and 83%, respectively) compared to the SGA tool. Furthermore, the reliability of the GLIM criteria for malnutrition diagnosis in hospitalized patients was acceptable in all 3 applied approaches (KR-20 &gt; 0.5). The malnutrition diagnosed by GLIM criteria could significantly predict the odds of prolonged hospital stays, 30-day hospital readmission, and 60-day mortality, while it had no significant association with the risk of hospital mortality.ConclusionThe current study revealed that applying GLIM criteria had satisfactory validity in diagnosing hospital malnutrition in non-critically ill hospitalized patients.
Angiotensin-I-converting enzyme inhibitory peptides from eel (Anguilla japonica) bone collagen: preparation, identification, molecular docking, and protective function on HUVECs
Authors: Xiang Huan , Huang Hui , Shao Yanqiu , Hao Shuxian , Li Laihao , Wei Ya , Chen Shengjun , Zhao Yongqiang 
Published: December 2024

IntroductionHypertension is a chronic cardiovascular disease, which can trigger some disease such as heart failure, loss of vision or kidney. There were various peptides derived from food that are recognized for their ability to inhibit ACE activity, potentially leading to a reduction in blood pressure levels in vivo. The primary objective of this research is to discover ACE inhibitory peptides from protein hydrolysates of eel bone collagen (EBCHs).MethodsTo begin, EBCHs were created and then divided through the process of ultrafiltration. The second step involved screening of peptides capable of inhibiting ACE by combining peptidomics and molecular docking. And the mechanism by which ACE interacts with peptides has been studied. Finally, the hypotensive mechanism of identified peptide through cell experiments with HUVEC (Human Umbilical Vein Endothelial Cells).ResultsEel (Anguilla japonica) bone collagen was hydrolyzed by alcalase and the hydrolysate was separated into three fractions, among which the F2 displayed a higher level of ACE inhibitory activity. According to molecular docking calculations, a total of 615 peptides were identified through nano-HPLC-MS/MS, with the prediction of seven newly discovered ACE inhibitory peptides (PMGPR, GPMGPR, GPAGPR, GPPGPPGL, GGPGPSGPR, GPIGPPGPR, GPSGAPGPR). Notably, GPPGPPGL had the lowest IC50 value of 535.84 μM among the identified peptides, indicating its potency as an ACE inhibitor. The ACE S2 pocket formed hydrogen and hydrophobic interactions with GPPGPPGL. Lineweaver-Burk plots revealed that GPPGPPGL competitively bound to ACE’s active site residues. Treatment with GPPGPPGL significantly increased nitric oxide secretion (p &lt; 0.01) and decreased endothelin-1 (ET-1) production in HUVECs.DiscussionOur findings suggest that combining peptidomics with molecular docking is effective for rapidly screening ACE inhibitory peptides. Future studies should assess the bioavailability and in vivo activity of the identified peptide GPPGPPGL from EBCHs.
Prognostic value of the HALP score in metastatic castration-resistant prostate cancer: an analysis combined with time to castration resistance
Authors: Gökmen İvo , Demir Nazan , Peker Pınar , Özcan Erkan , Akgül Fahri , Bayrakçı İsmail , Divriklioğlu Didem , Erdoğan Bülent , Topaloğlu Sernaz , Hacıoğlu Muhammet Bekir 
Published: December 2024

ObjectiveThe aim of our study was to assess the impact of the combination of HALP score with TTCR score on OS and PFS in PC patients who developed castration resistance.Patients and methodsThe study enrolled 152 patients with metastatic disease who had received either ARTAs or docetaxel as first-line treatment. The median cut-off was 30.83 months for the HALP score and 16.1 months for TTCR determined by ROC analysis. Based on these cut-off values, patients were categorized into low-high HALP score and TTCR &lt;16.1 months-TTCR ≥16.1 months groups. The combination of HALP score and TTCR was then stratified by risk into three new groups: Factor 0, Factor 1, and Factor 2.ResultsPFS was significantly shorter in the TTCR &lt;16.1 months group compared to the TTCR ≥16.1 months group, as well as in the low-HALP score group compared to the high-HALP score group. Furthermore, as the number of factors increased, a significant increase in OS and PFS was observed in the groups formed by the combination of HALP score and TTCR.ConclusionWe have validated the predictive capability of combining low HALP score (&lt;30.38) and short TTCR (&lt;16.1 months) parameters in estimating the OS and PFS durations of mCRPC patients, both recognized as unfavorable prognostic indicators.
Integrated bioinformatics and interaction analysis to advance chronotherapies for mental disorders
Authors: Bhatnagar Apoorva , Raj Gupta , Das Sandip , Kannihali Arpita , Rajakumara Eerappa , Murray Greg , Ray Sandipan 
Published: December 2024

IntroductionRobust connections have been identified between the pathophysiology of mental disorders and the functioning of the circadian system. The overarching objective of this study was to investigate the potential for circadian rhythms to be leveraged for therapeutics in mental disorders.MethodsWe considered two approaches to chronotherapy–optimal timing of existing medications (“clocking the drugs”) and redressing circadian abnormalities with small molecules (“drugging the clock”). We assessed whether circadian rhythm-modulating compounds can interact with the prominent drug targets of mental disorders utilizing computational tools like molecular docking and molecular dynamics simulation analysis.ResultsFirstly, an analysis of transcript-level rhythmic patterns in recognized drug targets for mental disorders found that 24-hour rhythmic patterns were measurable in 54.4% of targets in mice and 35.2% in humans. We also identified several drug receptors exhibiting 24-hour rhythmicity involved in critical physiological pathways for neural signaling and communication, such as neuroactive ligand-receptor interaction, calcium signaling pathway, cAMP signaling pathway, and dopaminergic and cholinergic synapses. These findings advocate that further research into the timing of drug administration in mental disorders is urgently required. We observed that many pharmacological modulators of mammalian circadian rhythms, including KL001, SR8278, SR9009, Nobiletin, and MLN4924, exhibit stable binding with psychotropic drug targets.DiscussionThese findings suggest that circadian clock-modulating pharmacologically active small molecules could be investigated further for repurposing in the treatment of mood disorders. In summary, the present analyses indicate the potential of chronotherapeutic approaches to mental disorder pharmacotherapy and specify the need for future circadian rhythm-oriented clinical research.
Yinzhihuang injection as adjuvant treatment for neonatal hyperbilirubinemia: a systematic review and meta-analysis of randomized clinical trials
Authors: Li Junqiang , Zhang Liyu , Chen Zhongguo , Qin Min 
Published: December 2024

BackgroundNeonatal hyperbilirubinemia (NH) is a common phenomenon for neonate inpatients, and Yinzhihuang (YZH) injection is a well-known Chinese herbal formula for the treatment of NH. This study aims to evaluate the efficacy of YZH injection on NH.MethodsA comprehensive literature search was performed in four Chinese databases [China National Knowledge Infrastructure (CNKI), Chinese Biomedical Literature (CBM), China Science Journal Database (VIP), and Wan Fang ] and four English language databases (PubMed, Web of Science, EMbase and the Cochrane Library) from inception to 16 April 2024. Randomized controlled trials on YZH injection for NH were collected. Review Manager 5.4 software was used for meta-analysis and bias risk assessment.ResultTwenty-two trials involving 2,714 neonates were included. Significant differences were detected between YZH injection group and control group for total effective rate (RR = 1.15, 95%CI: 1.11 to 1.20, p &lt; 0.00001), serum bilirubin level (the third day, MD = −36.80, 95%CI: −52.07 to −21.53; the fifth day, MD = −4.18, 95%CI: −7.50 to −0.86, p &lt; 0.00001), and time to jaundice resolution (MD = −2.46, 95%CI -2.81 to −2.10, p &lt; 0.00001). Adverse effects of YZH injection were described in four trials, with an incidence of 8.60%.ConclusionThe use of YZH injection as an adjuvant to NH appears to be safe and may offer superior benefits compared to routine treatment alone for NH. However, these potential benefits require confirmation in future trials employing rigorous methodology. The current evidence is not enough to recommend YZH injection as a routine treatment for NH.Systematic Review RegistrationPROSPERO (https://www.crd.york.ac.uk/prospero/), identifier, CRD42024556811.
Intergenerational support, activities of daily living, and the interaction on psychological distress in older adults
Authors: Zheng Juan , Xu Jianqiang , Wu Yuhang , Xu Shuo , Gao Yang 
Published: December 2024

ObjectivesThe purpose of this study is to examine the effects of intergenerational support and activities of daily living (ADL) on psychological distress in older adults, and to analyse the effects of the interaction between ADL and intergenerational support on psychological distress in older adults.MethodsA probability sample of 1,065 older adults aged 60 and above was conducted in Xuzhou, China, from 18 June to 26 August 2023. ADL was assessed using the ADL scale combined with the Barthel Index; Intergenerational support was measured using the Intergenerational Support Scale; and psychological distress was measured using the Kessler 10 scale. An ordered multichotomous logistic regression model was constructed to analyse the factors influencing psychological distress in the elderly. The interaction model was constructed by one-way analysis of variance to further analyse the effect of the interaction between financial support, instrumental support and emotional support and ADL on psychological distress in the elderly.ResultsElderly living in rural areas, with an annual household income of less than 30,000 yuan, who engaged in manual labor before retirement, who did not receive financial support and who received instrumental support were more likely to experience psychological distress, and the higher the degree of restriction in ADL, the higher the risk of psychological distress. A significant interaction was observed between ADL and financial support (F = 221.570, p &lt; 0.05), as well as between ADL and emotional support (F = 399.805, p &lt; 0.01). Additionally, a significant interaction was found among ADL, financial support, and instrumental support (F = 227.187, p &lt; 0.05).Conclusion(1) A significant association between place of residence, annual household income, occupation, financial support, instrumental support, ADL, and psychological distress in older adults; (2) When the level of ADL was moderate, the risk of psychological distress in older adults who received emotional support was significantly lower than that of older people who did not receive emotional intergenerational support; When the level of ADL was completely independent, financial support was beneficial in reducing psychological distress in the elderly; (3) the risk of psychological distress in the elderly who received instrumental support increased with the severity of ADL limitations, regardless of whether they received additional financial support.
The development of the Compassion Satisfaction and Compassion Fatigue scale
Authors: Halamová Júlia , Kanovský Martin , Krizova Katarina , Šoková Bronislava , Baránková Martina , Figley Charles 
Published: December 2024

BackgroundThere is a high probability of compassion fatigue occurring in helping professionals who work with traumatized clients or patients. Several instruments exist for measuring compassion fatigue, but all of them have methodological flaws. The original Compassion Satisfaction/Fatigue Self-Test for Helpers is time-consuming and its psychometric properties, including factor structure, have not been supported in the research.MethodsTherefore, the goal of this study was to apply a Mokken scale analysis for polytomous items to shorten the Compassion Satisfaction/Fatigue Self-Test for Helpers and improve its psychometric properties. In addition, we wanted to create norms for the helping professional population. The research sample consisted of 2,320 participants from various helping professions.ResultsTo improve scalability, most of the scale items were removed. The resulting item scalability coefficients ranged from 0.349 to 0.655 and Molenaar–Sijtsma reliability coefficient ranged between 0.75 and 0.87. The final revised and shortened Compassion Satisfaction and Compassion Fatigue scale (CSCFS) consisted of 5 items for the Compassion Satisfaction—Personal Integrity and Happiness subscale, 5 items for the Compassion Satisfaction—Work Competence and Happiness subscale, 9 items for the Compassion Fatigue—Secondary Traumatic Stress subscale, and 7 for the Compassion Fatigue—Burnout subscale. The newly revised subscales have good reliability coefficients.ConclusionThe CSCFS appears to be a valid and reliable instrument for assessing compassion satisfaction and compassion fatigue among helping professionals. More research is required to support its factor structure in a range of settings. We recommend testing usability across different helping professions and cultures.
Microbial and chemical stability of lumpfish (Cyclopterus lumpus) roe during refrigerated storage in acid-salt brines with or without antioxidants
Authors: Jensen Hanne Aarslev , Plaza Laura Garcia , Houmann Rannvá Høgnadóttir , Andreasen Pernille Bak , Bøknæs Niels , Kragh Martin Laage , Hansen Lisbeth Truelstrup , Mejlholm Ole , Jacobsen Charlotte , Dalgaard Paw , Sørensen Ann-Dorit Moltke 
Published: December 2024

Immediately after the harvest of lumpfish (Cyclopterus lumpus) roe in artic regions, a common practice is to preserve the fresh roe in brine to produce a salted intermediate product (SIP), which can be transported and stored refrigerated for up to 1 year prior to retail processing. Because the roe is susceptible to microbial growth and lipid oxidation, the brines have been composed of mixes of salt (&gt;10%) and benzoic acid to manage the quality and safety of the SIP. However, a demand for reducing the use of salt and benzoic acid is appearing on the European market. Therefore, the aim of this study was to evaluate the effect of five different brines comprised of combinations of salt, organic acids, and antioxidants on controlling microbial and chemical changes of the SIP, to be able to avoid the use of benzoic acid and reduce the salt content. Freshly harvested roe was mixed with brines to obtain 7 or 10% (w/w) NaCl and combinations of acetic, benzoic and/or lactic acids with or without addition of VivOx 7.5 (IFF Inc.) or Herbalox D-450 (Kalsec Inc.) as antioxidants. Brined roes were stored refrigerated (1.94°C ± 0.56°C) with sampling after 50, 80, 120, 210, 288 and 353 days. Regardless of the treatment applied, aerobic viable counts increased to &gt;6.1 log CFU/g after 120 days of storage and the microbiota in all treatments became dominated by Debaromyces hansenii as identified using MALDI-TOF and metagenome sequencing. The addition of antioxidants reduced the formation of lipid hydroperoxides and thiobarbituric acid reactive substances. Both Herbalox and VivOx reduced the formation of 1-penten-3-ol, whereas VivOx increased the formation of pentanal in the roe. Concentrations of 2-methyl-butanol and 3-methyl-butanol were markedly reduced by treatments that included benzoic acid, which may be related to differences in the activity of the microbiota. Herbalox was more efficient than VivOx in reducing oxidative changes whereas the effect of organic acids on oxidative alterations remained inconclusive. In conclusion, it was possible to reduce the salt content and avoid the use of benzoic acid without affecting the microbial and oxidative stability of the SIP as well as its safety.
Методы обнаружения помех при приеме сигналов глобальных навигационных спутниковых систем. Часть II
Authors: К.Ю. Коломенский 
Published: December 2024

Рассмотрены методы обнаружения непреднамеренных и преднамеренных помех глобальным навигационным спутниковым системам (ГНСС). Во второй части рассматриваются методы обнаружения преднамеренных имитационных помех ГНСС – спуфинга. Описывается методика проведения спуфинг-атак, предлагается вариант классификации методов обнаружения спуфинга, анализируются методы обнаружения спуфинга в порядке увеличения сложности их реализации от простых методов с использованием типовых приемных модулей ГНСС до антиспуфинговых комплексов на основе анализа пространственных характеристик сигналов с использованием антенных решеток.
Methods for detecting unintended and intentional interference to global navigation satellite systems (GNSS) are considered. The second part discusses methods of detecting intentional simulated GNSS interference – spoofing. The techniques of spoofing attacks are described, a classification of spoofing detection methods is proposed, and detection methods are analyzed in order of increasing complexity of their implementation from simple methods using typical GNSS receiving modules to anti-spoofing complexes based on the spatial analysis with antenna arrays.
Моделирование сигналов сетей связи с кодами Уолша в виде стохастических дифференциальных уравнений
Authors: Е.И. Глушанков , В.Я. Конторович , С.Г. Егоров 
Published: December 2024

Синтезированы стохастические дифференциальные уравнения для моделирования сигналов в сетях связи с использованием кодов Уолша. Предложена математическая модель сигналов, полученная при помощи уравнений ФоккераПланка-Колмогорова и методов марковских процессов, для описания нелинейных преобразований в групповых трактах. Исследуются сигналы кодов Уолша, обеспечивающие эффективную передачу данных. Представлена аппроксимация полученной плотности распределения случайного процесса на основе рядов Грамма-Шарлье. Проведено сравнение различных методов аппроксимации с целью оценки эффективности и точности моделирования сигналов. Представленные результаты предлагают удобную модель для статистической симуляции процессов обработки сигналов с целью оптимизации систем связи.
Stochastic differential equations for modeling signals in communication networks using Walsh codes are synthesized. A mathematical model of signals, obtained using Fokker-Planck-Kolmogorov equations and Markov process methods, is proposed to describe nonlinear transformations in group paths. Walsh code signals that provide efficient data transmission are investigated. An approximation of the obtained distribution density of a random process based on Gramm-Charlier series is presented. Different approximation methods are compared in order to evaluate the efficiency and accuracy of signal modeling. The presented results offer a convenient model for statistical simulation of signal processing processes in order to optimize communication systems.