bluuebunny/crossref_metadata_2025_split huggingface dataset:
Caveolin-1 as a pathophysiological factor and target in psoriasis
Authors: Kruglikov Ilja L. , Scherer Philipp E. 
Published: February 2019

AbstractLow expression of caveolin-1 (Cav-1) is typical in psoriatic lesions and overexpression of Cav-1 leads to a reduction of inflammation and suppression of epidermal hyperproliferation, thus ameliorating these two well-known hallmarks of psoriasis. At the same time, the interfacial layers of the white adipose tissue (WAT) adjacent to psoriatic lesions demonstrate much higher stiffness, which also points to a modification of Cav-1 expression in this tissue. These processes are connected with each other and regulated via exosomal exchange. Here we discuss the role of Cav-1 expression in inflammatory and hyperproliferative processes and analyze the ways to provide spatially different modulation of Cav-1 expression in the skin and WAT. Such modulation can be induced by different pharmacological and physical factors. These include application of mechanical stress and supra-physiological temperatures. Cav-1 should therefore be considered as an important target in treatment of psoriasis.
Asymmetric MXene/monolayer transition metal dichalcogenide heterostructures for functional applications
Authors: Li Baihai , Guo Haoran , Wang Yunrui , Zhang Wenxu , Zhang Qiuju , Chen Liang , Fan Xiaoli , Zhang Wanli , Li Yanrong , Lau Woon-Ming 
Published: February 2019

AbstractA versatile two-dimensional (2D) molecular bilayer heterostructure of asymmetric MXene/monolayer transition metal dichalcogenide (aMXene/mTMDC) with a high interfacial built-in electric field is here simulated, where aMXene is an aMXene with the top or bottom electronegative atom plane of MXene removed. The asymmetric structural design of aMXene leads to a high dipole moment perpendicular to the 2D molecular plane. Although the unpassivated metal atoms in the aMXene are unstable and electropositive, coupling them to the electronegative chalcogenide atoms in an aMXene/mTMDC bilayer resolves this deficiency. The dipole field tunable by the specific composition of aMXene/mTMDC is leveraged to engineer unusual band structures, band alignments, and charge redistribution/injection in the bilayer. The simulated design of several aMXene/mTMDC bilayers for possible use in spintronics, microelectronics/optoelectronics, and catalysis/photocatalysis are shown.
Characterization of Cellulose synthase-like D (CSLD) family revealed the involvement of PtrCslD5 in root hair formation in Populus trichocarpa
Authors: Peng Xiaopeng , Pang Hongying , Abbas Manzar , Yan Xiaojing , Dai Xinren , Li Yun , Li Quanzi 
Published: February 2019

AbstractCellulose synthase-like D (CSLD) family was characterized for their expression and functions in Populus trichocarpa. Ten members, PtrCslD1-10, were identified in the P. trichocarpa genome, and they belong to 4 clades by phylogenetic tree analysis. qRT-PCR and promoter:GUS assays in Arabidopsis and P. trichocarpa displayed divergent expression patterns of these 10 PtrCSLD genes in root hairs, root tips, leaves, vascular tissues, xylem and flowers. Among PtrCslD2, PtrCslD4, PtrCslD5, PtrCslD6, and PtrCslD8 that all exhibited expression in root hairs, only PtrCslD5 could restore the root hairless phenotype of the atcsld3 mutant, demonstrating that PtrCslD5 is the functional ortholog of AtCslD3 for root hair formation. Our results suggest more possible functions for other PtrCslD genes in poplar.
Long non-coding RNA repertoire and open chromatin regions constitute midbrain dopaminergic neuron - specific molecular signatures
Authors: Gendron J. , Colace-Sauty C. , Beaume N. , Cartonnet H. , Guegan J. , Ulveling D. , Pardanaud-Glavieux C. , Moszer I. , Cheval H. , Ravassard P. 
Published: February 2019

AbstractMidbrain dopaminergic (DA) neurons are involved in diverse neurological functions, including control of movements, emotions or reward. In turn, their dysfunctions cause severe clinical manifestations in humans, such as the appearance of motor and cognitive symptoms in Parkinson’s Disease. The physiology and pathophysiology of these neurons are widely studied, mostly with respect to molecular mechanisms implicating protein-coding genes. In contrast, the contribution of non-coding elements of the genome to DA neuron function is poorly investigated. In this study, we isolated DA neurons from E14.5 ventral mesencephalons in mice, and used RNA-seq and ATAC-seq to establish and describe repertoires of long non-coding RNAs (lncRNAs) and putative DNA regulatory regions specific to this neuronal population. We identified 1,294 lncRNAs constituting the repertoire of DA neurons, among which 939 were novel. Most of them were not found in hindbrain serotonergic (5-HT) neurons, indicating a high degree of cell-specificity. This feature was also observed regarding open chromatin regions, as 39% of the ATAC-seq peaks from the DA repertoire were not detected in the 5-HT neurons. Our work provides for the first time DA-specific catalogues of non-coding elements of the genome that will undoubtedly participate in deepening our knowledge regarding DA neuronal development and dysfunctions.
Effects and Components of Placebos with a Psychological Treatment Rationale – Three Randomized-Controlled Studies
Authors: Gaab Jens , Kossowsky Joe , Ehlert Ulrike , Locher Cosima 
Published: February 2019

AbstractIn recent years, placebos have evolved from a mean to control for ‘therapeutic chaff’ to something that has clinically relevant effects with biological underpinning and that is considered to have clinical as well as scientific potential. However, the wealth of scientific placebo research is conceptualized in a biomedical context, i.e. based on placebos provided with a biomedical treatment rationale, whereas little is known about effects and mechanisms of placebos provided with a psychological treatment rationale. This has important repercussions not only on placebo research, but also on attempts to establish specificity of psychological interventions, such as psychotherapy. Therefore, we set out to assess the effects and possible components of placebos provided with a psychological treatment rationale in three experiments on healthy subjects. We show that placebos provided with a psychological treatment rationale are effective in short- as well as mid-term, but only when provided by a trustworthy, friendly and empathetic experimenter. These findings indicate that placebos are effective outside the medical context and thus need be controlled for in non-medical trials. Furthermore, it highlights and confirms the importance of a plausible psychological treatment rationale in the context of a therapeutic alliance for psychological interventions, such as psychotherapy.
Aberrant DNA methylation of M1-macrophage genes in coronary artery disease
Authors: Bakshi Chetan , Vijayvergiya Rajesh , Dhawan Veena 
Published: February 2019

AbstractM1 and M2 macrophage balance in atherosclerosis has attracted much interest. Though, it remains unknown how macrophage heterogeneity is regulated. Moreover, the regulation of macrophage polarization and activation also involve DNA methylation. However, it remains ambiguous which genes are under direct regulation by DNA methylation. Our aim was to evaluate the gene-specific promoter DNA methylation status of M1/M2 polarization markers in PBMCs of CAD patients. A case-control study was performed with 25 CAD patients and 25 controls to study the promoter DNA methylation status of STAT1, STAT6, MHC2, IL12b, iNOS, JAK1, JAK2 and SOCS5 using MS-HRM analysis. Our data indicates that there was a clear-cut difference in the pattern of gene-specific promoter DNA methylation of CAD patients in comparison to controls. A significant difference was observed between the percentage methylation of STAT1, IL12b, MHC2, iNOS, JAK1 and JAK2 in CAD patients and control subjects. In conclusion, our data show that MS-HRM assay is a rapid and inexpensive method for qualitatively identifying aberrant gene-specific promoter DNA methylation changes in CAD. Furthermore, we propose that gene-specific promoter DNA methylation based on monocyte/macrophage might aid as diagnostic marker for clinical application or DNA methylation-related drug interventions may offer novel possibilities for atherosclerotic disease management.
Structure-efficiency relationship of photoinduced electron transfer-triggered nitric oxide releasers
Authors: Ieda Naoya , Oka Yumina , Yoshihara Toshitada , Tobita Seiji , Sasamori Takahiro , Kawaguchi Mitsuyasu , Nakagawa Hidehiko 
Published: February 2019

AbstractSpatiotemporally controllable nitric oxide (NO) releasers are required for biological studies and as candidate therapeutic agents. Here, we investigate the structure-efficiency relationship of a series of photoinduced electron transfer-triggered NO releasers based on our reported yellowish-green light-controllable NO releaser, NO-Rosa. The distance between the NO-releasing N-nitrosoaminophenol moiety and the rosamine antenna moiety was critical for efficient NO release. Notably, substitution at the phenolic hydroxyl group blocked NO release. We synthesized NO-Rosa-Gal bearing D-galactose (Gal) at this location, and showed that hydrolysis by β-galactosidase restored the photoresponse. This represents proof-of-concept of a strategy for highly specific control of NO release by using a double-lock system involving both enzymatic reactivation and photo-control.
Low efficiency of large volcanic eruptions in transporting very fine ash into the atmosphere
Authors: Gouhier Mathieu , Eychenne Julia , Azzaoui Nourddine , Guillin Arnaud , Deslandes Mathieu , Poret Matthieu , Costa Antonio , Husson Philippe 
Published: February 2019

AbstractVolcanic ash clouds are common, often unpredictable, phenomena generated during explosive eruptions. Mainly composed of very fine ash particles, they can be transported in the atmosphere at great distances from the source, having detrimental socio-economic impacts. However, proximal settling processes controlling the proportion (ε) of the very fine ash fraction distally transported in the atmosphere are still poorly understood. Yet, for the past two decades, some operational meteorological agencies have used a default value of ε = 5% as input for forecast models of atmospheric ash cloud concentration. Here we show from combined satellite and field data of sustained eruptions that ε actually varies by two orders of magnitude with respect to the mass eruption rate. Unexpectedly, we demonstrate that the most intense eruptions are in fact the least efficient (with ε = 0.1%) in transporting very fine ash through the atmosphere. This implies that the amount of very fine ash distally transported in the atmosphere is up to 50 times lower than previously anticipated. We explain this finding by the efficiency of collective particle settling in ash-rich clouds which enhance early and en masse fallout of very fine ash. This suggests that proximal sedimentation during powerful eruptions is more controlled by the concentration of ash than by the grain size. This has major consequences for decision-makers in charge of air traffic safety regulation, as well as for the understanding of proximal settling processes. Finally, we propose a new statistical model for predicting the source mass eruption rate with an unprecedentedly low level of uncertainty.
Author Correction: Mechanochromism induced through the interplay between excimer reaction and excited state intramolecular proton transfer
Authors: Wei Yu-Chen , Zhang Zhiyun , Chen Yi-An , Wu Cheng-Ham , Liu Zong-Ying , Ho Ssu-Yu , Liu Jiun-Chi , Lin Jia-An , Chou Pi-Tai 
Published: February 2019

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Population projections for U.S. counties by age, sex, and race controlled to shared socioeconomic pathway
Authors: Hauer Mathew E. 
Published: February 2019

AbstractSmall area and subnational population projections are important for understanding long-term demographic changes. I provide county-level population projections by age, sex, and race in five-year intervals for the period 2020–2100 for all U.S. counties. Using historic U.S. census data in temporally rectified county boundaries and race groups for the period 1990–2015, I calculate cohort-change ratios (CCRs) and cohort-change differences (CCDs) for eighteen five-year age groups (0–85+ ), two sex groups (Male and Female), and four race groups (White NH, Black NH, Other NH, Hispanic) for all U.S counties. I then project these CCRs/CCDs using ARIMA models as inputs into Leslie matrix population projection models and control the projections to the Shared Socioeconomic Pathways. I validate the methods using ex-post facto evaluations using data from 1969–2000 to project 2000–2015. My results are reasonably accurate for this period. These data have numerous potential uses and can serve as inputs for addressing questions involving sub-national demographic change in the United States.
Drug-Eluting Balloons and Drug-Eluting Stents in the Treatment of Peripheral Vascular Disease
Authors: Lindquist Jonathan , Schramm Kristofer 
Published: February 2019

AbstractIn the last 20 years, peripheral artery disease (PAD) has been increasingly recognized as a significant cause of morbidity and mortality in the United States. The endovascular treatment of PAD has seen a marked rise as minimally invasive techniques and devices have been refined. Two newer devices, drug-eluting stents and drug-eluting balloons, are on the forefront of the battle against limb loss from PAD. This review focuses on the data backing the use of drug-eluting technologies for use in the peripheral arterial system.
Development and Formative Assessment of the Hearing Loss Toolkit for Self-Management
Authors: Oree Preyanca , Sanchez Victoria , Reed Nicholas , Chisolm Theresa , Arnold Michelle 
Published: February 2019

AbstractHearing aids are a demonstrated efficacious intervention for age-related hearing loss, and research suggests that good hearing loss self-management skills improve amplification satisfaction and outcomes. One way to foster self-management skills is through the provision of patient education materials. However, many of the available resources related to the management of hearing loss do not account for health literacy and are not suitable for use with adults from varying health literacy backgrounds. To address this issue, we developed the Hearing Loss Toolkit for Self-Management as part of a manualized, best practices hearing intervention used in large clinical trial. We incorporated health literacy recommendations from the U.S. Department of Health and Human Services, the Centers for Disease Control and Prevention, and the Agency for Healthcare Research and Quality in a series of modules that address a variety of common problem areas reported by adults with hearing loss. A formative assessment consisting of feedback questionnaires, semistructured interviews, and a focus group session with representatives from the target audience was conducted. Findings from the development assessment process demonstrate that the Hearing Loss Toolkit for Self-Management is suitable for use with adults with age-related hearing loss who have varying health literacy backgrounds and abilities.
Superior Sagittal Sinus Invasion by Malignant Glioma: Case Report and Literature Review
Authors: Figueiredo Gonçalo , Moreira Sérgio , Pinheiro Célia , Calheiros Alfredo 
Published: February 2019

AbstractAnaplastic oligodendrogliomas (AOs) correspond to ∼ 23% of all oligodendrogliomas. They correspond to a tumor with malignant histological characteristics, focal or diffuse, associated with a worse prognosis. In the present case report, we describe the case of a 30-year-old female submitted to resection of a right parietal lesion whose histology showed to be an AO. She underwent complementary treatment with chemotherapy and radiotherapy according to the Roger Stupp protocol. Four years after the initial diagnosis, there was tumor recurrence within the superior sagittal sinus, with no evidence of recurrence elsewhere. In the literature, we have found no similar published case reinforcing the rarity of this condition
Identification of the Regulatory Elements and Target Genes of Megakaryopoietic Transcription Factor MEF2C
Authors: Kong Xianguo , Ma Lin , Chen Edward , Shaw Chad , Edelstein Leonard 
Published: February 2019

AbstractMegakaryopoiesis produces specialized haematopoietic stem cells in the bone marrow that give rise to megakaryocytes which ultimately produce platelets. Defects in megakaryopoiesis can result in altered platelet counts and physiology, leading to dysfunctional haemostasis and thrombosis. Additionally, dysregulated megakaryopoiesis is also associated with myeloid pathologies. Transcription factors play critical roles in cell differentiation by regulating the temporal and spatial patterns of gene expression which ultimately decide cell fate. Several transcription factors have been described as regulating megakaryopoiesis including myocyte enhancer factor 2C (MEF2C); however, the genes regulated by MEF2C that influence megakaryopoiesis have not been reported. Using chromatin immunoprecipitation-sequencing and Gene Ontology data we identified five candidate genes that are bound by MEF2C and regulate megakaryopoiesis: MOV10, AGO3, HDAC1, RBBP5 and WASF2. To study expression of these genes, we silenced MEF2C gene expression in the Meg01 megakaryocytic cell line and in induced pluripotent stem cells by CRISPR/Cas9 editing. We also knocked down MEF2C expression in cord blood-derived haematopoietic stem cells by siRNA. We found that absent or reduced MEF2C expression resulted in defects in megakaryocytic differentiation and reduced levels of the candidate target genes. Luciferase assays confirmed that genomic sequences within the target genes are regulated by MEF2C levels. Finally, we demonstrate that small deletions linked to a platelet count-associated single nucleotide polymorphism alter transcriptional activity, suggesting a mechanism by which genetic variation in MEF2C alters platelet production. These data help elucidate the mechanism behind MEF2C regulation of megakaryopoiesis and genetic variation driving platelet production.
Modification of excitation and charge transfer in cavity quantum-electrodynamical chemistry
Authors: Schäfer Christian , Ruggenthaler Michael , Appel Heiko , Rubio Angel 
Published: February 2019

Significance
Excitation and charge transfer are fundamental processes in nature, and controlling these processes is a major goal of quantum chemistry. While these processes are well understood for the usual free-space case, when the electromagnetic vacuum is changed due to, e.g., a cavity, these processes can be dramatically different. We consider these changes in transfer processes with real-space donor–acceptor models, where we put an emphasis on the impact of electron–electron correlations. We find results in line with recent experiments, where strong light–matter interaction leads to enhanced transfer reactions, even when in the corresponding free-space situation no transfer should be possible. We highlight that the processes depend crucially on the Coulomb and self-polarization interactions.
Infiltration of CD8
+
T cells into tumor cell clusters in triple-negative breast cancer
Authors: Li Xuefei , Gruosso Tina , Zuo Dongmei , Omeroglu Atilla , Meterissian Sarkis , Guiot Marie-Christine , Salazar Adam , Park Morag , Levine Herbert 
Published: February 2019

Significance
The infiltration of cytotoxic T cells into tumors is a critical factor in immunotherapy efficacy. We developed an algorithm to quantify infiltration level using whole-section immunohistofluorescence images from different patients. We observed consistent infiltration patterns of cytotoxic T cells (on the tumor cell cluster level) at the core and margin of each tumor. These spatial distributions enabled us to generate several hypotheses for mechanisms underlying the various infiltration levels. We used mathematical models to test which hypothetical mechanisms could recapitulate these patterns. One hypothesis, that of a fibrotic barrier, was shown to be unlikely. The other, involving a T cell chemorepellent, agrees with the data and can be tested in future experiments that directly measure the chemokine spatial patterns.
Differences in Human Leukocyte Antigen Expression Between Breast Implant–Associated Anaplastic Large Cell Lymphoma Patients and the General Population
Authors: Tevis Sarah E , Hunt Kelly K , Miranda Roberto N , Lange Caitlin , Butler Charles E , Clemens Mark W 
Published: February 2019

Abstract

Background
Breast implant–associated anaplastic large cell lymphoma (BIA-ALCL) is an uncommon T-cell lymphoma associated with textured-surface breast implants. Human leukocyte antigen (HLA) polymorphisms have been described with other forms of lymphoma, but have not been described for BIA-ALCL.

Objectives
The aim of this study was to evaluate HLA polymorphisms in BIA-ALCL patients.

Methods
We prospectively evaluated HLA alleles in patients with BIA-ALCL. HLA was analyzed by probe-based sequence-specific testing and sequence-based typing. The frequencies of HLA-A, HLA-B, HLA-C, HLA-DRB1, and HLA-DQB1 alleles were evaluated. Allele frequencies in the Caucasian European general population were obtained from the National Marrow Donor Program to serve as normative controls. We estimated the relative risk of BIA-ALCL with 95% confidence intervals from a t test.

Results
Thirteen patients who had undergone BIA-ALCL and HLA testing were identified from 2017 to 2018. Patients carried 10, 11, and 9 HLA-A, HLA-B, and HLA-C alleles, respectively. There were 8 DRB1 alleles and 5 DQB1 alleles in the BIA-ALCL patients. The A*26 allele occurred significantly more frequently in the general population compared with BIA-ALCL patients (0.2992 vs 0.07692, P &lt; 0.001).

Conclusions
Our results identify a difference between HLA A*26 in patients who develop BIA-ALCL and the general population, and may signify genetic susceptibility factors responsible for germline genetic variation in HLA in patients with BIA-ALCL. Further work is needed to elucidate if these alleles are predictive for BIA-ALCL in women with textured-surface breast implants.
Level of Evidence: 4