bluuebunny/crossref_metadata_2025_split huggingface dataset:
The influence of the ratio of liver cells and bone marrow in the implantable cell-engineering structures of the liver on the recovery efficiency of functional and morphological parameters in chronic liver failure
Authors: Shagidulin M. Yu. , Onishchenko N. A. , Krasheninnikov M. E. , Nikolskaya A. O. , Volkova E. A. , Iljinsky I. M. , Mogeiko N. P. , Sevastianov V. I. , Gautier S. V. 
Published: May 2019

Aim:to determinate the most effective liver cells and multipotent mesenchymal stromal cells of bone marrow (MMSC BM) ratio into implantable cell engineering constructions (CECs) used for chronic liver failure (CLF) correcting.Materials and methods.For creating liver CECs it was used a biopolymer implant – a composition of a heterogeneous collagen-containing gel (BMCG) (Sphero®GEL trademark) containing viable liver cells and MMSC BM in the following ratios – 1 : 1; 5 : 1 and 10 : 1 respectively. CECs with different ratios of liver cells and MMSC BM were implanted into liver of rats in which chronic liver failure (CLF), was modeled by using CCl4. The effectiveness of the regulatory effects of CECs (with different cell ratios) on regenerative processes in livers were assessed by using biochemical, morphological and morphometric methods at different periods after their implantation.Results.Corrective effect of CECs with different cell composition on biochemical and morphological parameters of livers at chronic liver failure was established. During studying the liver CECs with various cell ratios of liver cells and MMSC BM (1 : 1; 5 : 1 and 10 : 1 respectively), it was found that the most optimal ratio of cells into the CECs is 5 : 1, because at this ratio of cells, there were a more distinct normalization of the morphological and functional liver parameters within 365 days after modeling CLF and maintenance of the structural homeostasis into the CECs. Themselves, which allows predicting their long-term regulatory effect on the liver tissue in CLF and maintaining its normal structural and functional state.Conclusion.The effective correction of chronic liver failure can be carried out by using the implanted liver CECs, in which donor liver cells and MMSC BM where presented in ratios – 1 : 1; 5 : 1 and 10 : 1. But analysis of prolonged correction of liver morphological and functional parameters at CECs using it was allow to recommend the preferences using of CECs with ratio 5 : 1, because prolonged preservation of structural homeostasis into these CECs makes possible to prognosticate their prolonged regulatory action on the liver tissue at CLF, especially for recipients on a waiting list for liver transplantation.
ThinPrep ve Konvansiyonel Yöntem ile Çalışılan Servikal Smear sonuçların Değerlendirilmesi
Authors: SEMİZ Altuğ , OZBAY Koray , YILANLIOGLU Cihangir , DENİZ Alparslan , ARİSOY Resul 
Published: May 2019

Amaç: Hastanemizde yapılmış olan servikal smear sonuçlarının yıllara ve hasta yaşına göre değerlendirilmesi, anormal sonuç oranlarının ve dağılımının araştırılması, kullanılan inceleme yöntemine göre sonuç dağılımında farklılık olup olmadığının belirlenmesi ve sonuçların ülkemiz epidemiyolojik verilerine katkıda bulunmasıdır.Materyal ve Metot: 2010 Haziran-2014 Aralık tarihleri arasında Şişli Memorial Hastanesi jinekoloji polikliniğine başvuran ve servikal smear testi yapılan hastaların sonuçlarını retrospektif olarak analiz ettik. Çalışmamıza 21-65 yaş arası 32.617 hasta dahil edildi. Bulgular: Olguların dağılımı incelendiğinde 29.831 (%91,5) olgunun patoloji sonucu normal, 1.713 olguda (%5,25) ASCUS, 135 olguda (%0,4) ASC-H, 21 olguda (%0,06) AGC, 767 olguda (%2,35) LSIL, 131 olguda (%0,4) HSIL, üç olguda adeno kanser ve iki olguda da skuamöz kanser tespit edilmiştir. Tüm olgular değerlendirildiğinde anormal sonuç oranımız %8,5 olarak saptanmıştır. LSIL (35,9±8,0 yıl) ve HSIL (34,4±7,2 yıl) olgularının anlamlı olarak daha genç yaşlarda olduğu saptandı. Sonuç: Çalışmamızda, ThinPrep yöntemi ile yıllar bazında prekanseröz lezyon saptanma oranlarında bir artış saptadık.
Две в одной
Authors: Боков Андрей Владимирович 
Published: May 2019

Modern architecture is not something that had been born from nothing at the turn of 20th century as somebody thinks. The roots of this phenomenon derive from the profane paradigm which constantly stays in a dialogue with the Sacred one and goes through all the history of human settlements.
The remarkable events of architectural history and the current practice are the consequences of the relations (domination or balance) between the Sacred (high, ideal) and the profane (practical, functional) paradigms.
Основные тенденции жилищной экономики российских городов
Authors: Косарева Надежда Борисовна , Пузанов Александр Сергевич , Полиди Татьяна Дмитриевна 
Published: May 2019

Urban environment quality and quality of living patterns are the key factors for Russian cities’ competitiveness increase. They both are to a large extent driven by the housing economy characteristics. The article provides analysis of basic trends in housing economy of Russian cities including:

housing consumption;
structure of housing and ownership rights;
housing price dynamics and the level of housing affordability;
condition of housing stock and utility infrastructure, investment in renovation of the housing stock;
state of competition at hosing market and supply elasticity to demand for differentiated and good quality urban environment;
state regulation of the housing economy — support for demand and supply, taxation policies.
Youtube como herramienta de marketing turístico de Galicia
Authors: Crespo Pereira Verónica 
Published: May 2019

A pesar de que Youtube nació como un instrumento social con el paso del tiempo ha evolucionado hasta convertirse en una herramienta de “social media marketing”. La presente investigación toma como objeto de estudio el uso de la plataforma Youtube como herramienta de marketing turístico de Galicia desde el punto de vista de organizaciones públicas y privadas y de usuarios particulares. Con el fin de determinar la finalidad de los vídeos para cada tipo de usuario, (organizaciones públicas turísticas, empresas turísticas y usuarios particulares), se llevó a cabo un análisis de contenido de 80 vídeos que permitieron establecer una categorización sobre los vídeos difundidos relacionados con el turismo de Galicia y las claves sobre las preferencias de los espectadores sobre dichos vídeos.
Paralelamente, se analizó la identidad proyectada de Galicia en Youtube a partir de los cinco vídeos más vistos relacionados con la región, así como la imagen percibida por los espectadores sobre dichos vídeos.
El trabajo cooperativo en base al modelo del cerebro total: una experiencia docente
Authors: Estrada Marta , Monferrer Diego , Segarra Mercedes , Moliner Miguel Angel 
Published: May 2019

Las exigencias planteadas en el Espacio Europeo nos llevan a plantearnos fórmulas que fomenten el aprendizaje cooperativo en el aula. En este trabajodescribimos una experiencia docente en la que, basándonos en el Modelo del Cerebro Total de Herrmann (1989), se forman los equipos de trabajo (con perfiles homogéneos y heterogéneos) para el desarrollo práctico de la asignatura Técnicas de Ventas del Grado de ADE. Los objetivos a alcanzar son tres: 1) fomentar la cooperación; 2) analizar el funcionamiento, rendimiento y satisfacción de los equipos de trabajo; 3) obtener información sobre cómo diseñar equipos eficientes y optimizar sus recursos. El objetivo, en última instancia, es ajustar nuestros métodos de enseñanza a los estilos de aprendizaje de los alumnos.
Rituximab and Immune Molecule Modulation in Burkitt’s Lymphoma Cell Lines
Authors: Quilty Rebecca , Smith Kendra , Jones David , Drover Sheila 
Published: May 2019

Objectives: Burkitt’s lymphoma is an aggressive B cell malignancy that is associated with EBV infection. The monoclonal antibody rituximab is used to treat many B cell malignancies, including Burkitt’s lymphoma. Studying immune molecule modulation in Burkitt’s lymphoma allows insight on developing new or refining existing immunotherapy agents for refractory or chemotherapy-resistant patients. The main purpose of this study was to explore rituximab’s impact on expression of immune molecules associated with immune activation or immune inhibition.
Methods: Burkitt’s lymphoma cell lines Raji, Ramos, Bjab, and an EBV-transformed B cell line, COX, were cultured and treated with an optimally-determined concentration of rituximab or human IgG for 24 hours. Immune modulation was determined by flow cytometric analysis of HLA-I, HLA-DR, PD-L1, and CD40. Three experiments were conducted for Raji, Ramos, Bjab, while 2 experiments were conducted for COX.
Results: Treatment of cells with rituximab, 10 µg/ml, completed downregulated CD20 expression and modulated expression of immune molecules. Compared to human IgG control, rituximab treatment decreased HLA-1, HLA-DR and CD40 expression on all cell lines, but significantly only for HLA-I on Bjab. Interestingly, the immune inhibitor PD-L1 was decreased on EBV-positive COX and Raji, but increased on EBV-negative Ramos and Bjab.
Conclusion: Downregulation of HLA-I could contribute to an immune escape mechanism. As this was a small study, there is limited transferability of the results to the clinical setting and further experiments are needed.
Cutting-edge Innovations in Cardiac Health: Galvanic Insights from a Clinician-Scientist, Dr. Benjamin Hibbert
Authors: Khan Faizan , Staibano Phillip , Deng Mimi , Fei Linda Yi Ning 
Published: May 2019

Dr. Benjamin Hibbert, MD, PhD, FRCPC is an interventional cardiologist, an assistant professor, as well as the director of the Vascular Biology and Experimental Medicine Laboratory at the University of Ottawa Heart Institute (UOHI). With a focus on performing revolutionary bench-to-bedside research, Dr. Hibbert’s clinical and basic science research interests include the development of novel cardiac biomarkers, elucidating the mechanisms that underlie pathological arterial remodelling in transplant vasculopathy, and the pharmacodynamics of adjuvant antiplatelet and antithrombotic agents in cardiac disease. We had the privilege of speaking with Dr. Hibbert about his career path, research experiences, and perspectives on the importance of the clinician-investigator program in training the oncoming generation of clinician-scientists.
Effects of Salacia oblonga on aluminium induced changes in membrane ATPases in brain cortex and hematological parameters in Wistar albino rats
Authors: C. M. Divyashanthi , A. Nandhini , Datchanamurty Barathane 
Published: May 2019

Background: The current study was done to evaluate the effect of hydro alcoholic extract of Salacia oblonga on aluminum induced toxicity in brain cortex and blood in Wistar albino rats.Methods: The experimental animals were divided into four groups, each group comprising of six animals for 36 days of experimental duration. We investigated Na+/K+, Mg2+, Ca2+ ATPases enzyme activity in brain cortex and hematological changes if any, upon administration of aluminum chloride (Alcl3) (300 mg/kg b.w), hydro alcoholic extract of Salacia oblonga (67 mg/kg b.w) and Alcl3+ Salacia oblanga with control (distilled water).Results: In brain cortex, Salacia caused an increased in activity of ATPases. Combined administration of Salacia suppressed the influence of aluminum on the ATPases in the brain cortex (p<0.05). Both aluminum as well as Salacia did not cause any alteration in the hemoglobin content of blood in Wistar albino rats. The erythrocytes count was also not altered by treatment with either aluminum or Salacia. Combined treatment with Salacia suppressed the influence of aluminum with reference to neutrophil count and significant increases in monocyte as well as lymphocyte count were seen. Thus, repeated administration of aluminum causes a decrease in neutrophil and increase in lymphocyte count.Conclusions: All the ATPases in brain cortex were found to be affected by aluminum administration and Salacia is found to counteract the ATPase effect to a particular extent implying the presence of an active principle that can counteract the aluminium toxicity indicating its possible usefulness in aluminum toxicity.
Study of fetomaternal outcome in second stage caesarean section
Authors: Goswami Kamal D. , Parmar Manisha M. , Kunjadiya Avnika N. 
Published: May 2019

Background: The incidence of second stage caesarean section is more in developing countries. Caesarean sections done at full cervical dilatation with impacted fetal head are difficult and associated with an increased incidence of maternal and fetal complications.Methods: This was prospective observational study conducted at a tertiary teaching institute. All second stage caesarean sections performed between September 2017 to August 2018 were analysed in terms of incidence, indications of caesarean-section, intra-operative and postoperative complications, maternal and fetal outcome.Results: During the study period there were total 7270 deliveries. Out of this 1884 deliveries were done by caesarean section. Out of them 50 were 2nd stage caesarean sections contributing to 2.65% of total sections. Patwardhan method was used in 50% cases for deliveryof deeply engaged head. Intra-operative complications were higher in terms of atonic pph (8%), extension of uterine incision (16%), in 3 cases bladder injury was noticed. Obstetric hysterectomy was required in 4% cases. 14% cases had postoperative febrile illness and 8% cases had wound infection. 44% babies required NICU admissions and neonatal death was 18%.Conclusions: Caesarean section in the 2nd stage of labour is associated with significantly increased maternal morbidity. Neonatal morbidity and mortality also increases. A proper judgement is required by a skilled obstetrician to take a decision for caesarean section at full cervical dilatation.
Clinical profile of children with tuberculosis from a semi urban referral centre in South India: a prospective observational study
Authors: G. Anuradha , G. Muraleetharan 
Published: May 2019

Background: Global TB report 2018 reports that in India, an estimated 2.2 lakh children become ill with tuberculosis (TB) each year. In spite of new rapid diagnostic methods, lack of gold standard test for confirming childhood tuberculosis remains an obstacle for the effective reporting of childhood TB and hence these cases often remain under diagnosed. The main objective was to study the clinical profile of childhood tuberculosis with relevant laboratory investigations aiding in early diagnosis.Methods: A prospective observational hospital-based study was conducted in IRT-Perundurai medical college hospital from April 2016 to March 2018. Children less than 18 years of age with tuberculosis were included in the study. Demographic details, presenting symptoms, family history of TB contact, nutritional status and clinical examination findings were documented. Complete blood count, ESR, Mantoux test, chest X-ray and sputum analyses for AFB were done for all patients.Results: Out of the total 124 children studied, the most common age of presentation of tuberculosis was >10 years of age (n=58, (46.8%)). There was a female preponderance (n=71 (57.25%)). Pulmonary tuberculosis (n= 87, (70.16%)) was the most common form followed by extra pulmonary (n=32, (25.80%)) and disseminated type (n=5, (4%)). Lymph node TB was the most common manifestation (n=20, 62.50%) among extra pulmonary form. Cough (n=75, 60.5%) with sputum (n=55,44.4%) were the predominant symptoms noted followed by fever (n=53, 42.7%). Bacteriological diagnosis was possible in 19.5% (n=17) patients only.Conclusions: This study reinforces that the diagnosis of childhood tuberculosis is based on the constellation of symptom evaluation, contact history, clinical examination, with relevant laboratory investigations.
Organizational commitment, job satisfaction, organizational justice and self-efficacy among nurses
Authors: Chegini Zahra , Janati Ali , Asghari-Jafarabadi Mohammad , Khosravizadeh Omid 
Published: May 2019

Background & Aim: Nursing shortage is a growing global challenge in healthcare organizations. Promoting nurses’ organizational commitment may help alleviate nursing shortage. This study sought to evaluate the relationships between organizational commitment, job satisfaction, organizational justice, and self-efficacy among nurses.
Materials & Methods: This cross-sectional study was conducted on 401 Iranian nurses randomly selected through two-stage cluster sampling. Data were collected using self-administered questionnaires and analyzed using the SPSS (v.17.0) and the Amos (v.17.0) software.
Results: The goodness of fit indices were as the following: χ2/df=2.76 (P<0.001), GFI=0.93; AGFI=0.87, NFI=0.96, RMSEA=0.068, and CFI=0.95. Organizational commitment had significant positive relationships with self-efficacy (β3=0.28, P<0.001) and job satisfaction (γ3=0.73, P<0.001), while organizational justice had significant positive relationship with job satisfaction (γ2=0.89, P<0.001). Moreover, job satisfaction had a mediating role in the relationship of organizational justice with organizational commitment.
Conclusion: Hospital managers can promote nurses’ organizational commitment through employing strategies to enhance their perceived organizational justice and thereby, improving their job satisfaction.
Collaborative Filtering Using Fuzzy Clustering for Categorical Multivariate Data Based on q-Divergence
Authors: Kondo Tadafumi ,   Shibaura Institute of Technology 3-7-5 Toyosu, Koto, Tokyo 135-8548, Japan, Kanzawa Yuchi 
Published: May 2019

In this study, a collaborative filtering method that uses fuzzy clustering and is based on q-divergence is proposed for categorical multivariate data. The results of experiments conducted on an artificial dataset indicate that the proposed method is more effective than the conventional one if the number of clusters and the initial setting are adequately set. Furthermore, the results of the experiments conducted on three real datasets indicate that the proposed method outperforms the conventional method in terms of	recommendation accuracy as well.
Transcriptional characterisation of the Aiptasia pallida pedal disc
Authors: Davey Peter A , Rodrigues Marcelo , Clarke Jessica L , Aldred Nick 
Published: May 2019

Abstract
Background

Biological adhesion (bioadhesion), enables organisms to attach to surfaces as well as to a range of other targets. Bioadhesion evolved numerous times independently and is ubiquitous throughout the kingdoms of life. To date, investigations have focussed on various taxa of animals, plants and bacteria, but the fundamental processes underlying bioadhesion and the degree of conservation in different biological systems remain poorly understood. This study had two aims: 1) To characterise tissue-specific gene regulation in the pedal disc of a basal metazoan; the model cnidarian Aiptasia pallida, and 2) to elucidate the specific genes involved in pedal disc adhesion.

Results

Five hundred and forty-seven genes were differentially expressed in the pedal disc compared to the rest of the animal. Four hundred and twenty-seven genes were significantly upregulated and one hundred and twenty genes were significantly downregulated. Forty-one condensed gene ontology terms and 19 protein superfamily classifications were enriched in the pedal disc. Eight condensed gene ontology terms and 11 protein superfamily classifications were depleted. Enriched superfamilies were consistent with classifications identified previously as important for the bioadhesion of unrelated marine invertebrates. A host of genes involved in regulation of extra cellular matrix generation and degradation were identified, as well as others related to development and immunity. Ab initio prediction identified 173 upregulated genes that putatively code for extracellularly secreted proteins.

Conclusion

The analytical workflow facilitated identification of genes involved in adhesion, immunity, defence and development of the A. pallida pedal disc. When defence, immunity and development-related genes were identified, those remaining corresponded most closely to formation of the extracellular matrix (ECM), implicating ECM in the adhesion of anemones to surfaces. This study therefore provides a valuable high-throughput resource for the bioadhesion community and lays a foundation for further targeted research to elucidate bioadhesion in the Cnidaria.
Molecular Detection and Glycoprotein B (UL55) Genotyping of Cytomegalovirus among Sudanese Renal Transplant Recipients
Authors: Ahmed Hind Haidar , Omer Alfadil Elobeid , Altyab Hisham N. 
Published: May 2019

Abstract
Background

Cytomegalovirus (CMV) is the most common opportunistic pathogen among solid organ transplant recipients’ especially renal transplants with significant morbidity and mortality. This study was designed to detect CMV DNA and to determine the frequency of different glycoprotein B (UL55) genotypes among Sudanese renal transplant recipients.

Methods

One hundred and four renal transplant recipients were included in this study. A blood specimen was collected from each recipient. DNA was extracted from plasma using QIAamp DNA mini kit. CMV amplification and quantification (estimation of viral load) was performed using CMV Real – RT Quant kits. Genotyping of Human CMV gB was carried out by nested PCR and sequencing of the highly diverse region of glycoprotein B.

Results

Cytomegalovirus (CMV) DNA (viremia) was detected in 40/104 (38.5%) of renal transplant recipients. The average of CMV DNA viral load was 358 x104 copies/ml (6.5 log10) ranged from 62 copies/ml (1.8 log 10) to 1.43x108 copies/ml (9 log10). CMV viremia was detected in (60%) of recipients of less than 1-12 months, (17%) of 13-24, (10%) of 25-36, (5%) of 37- 48 and (8%) in more than 48 months post-transplantation with no significant association (P. value = 0.296) between CMV viremia and post renal transplantation time. The association between the type of immunosuppressive drugs and high viral loads (more than 1000 copies /ml) showed a significant difference (P. value =0.05). The correlation between CMV loads of more than 1000 copies/ml and the presence of symptoms of CMV disease were highly significant (P.value =0.00). Fever 7(41%), fever and leucopenia 6(35%) and gastrointestinal disease 4(24%) were the most common presenting symptoms of CMV disease. CMV-genotyping revealed 8 cases (80%) for gB3, and 2 cases (20%) for gB4 genotypes. The most frequent genotype among Sudanese renal transplant recipients was gB3 and no mixed genotypes were observed.

Conclusions

The frequency of CMV DNA is high among Sudanese renal transplant recipients. CMV viremia viral loads were slightly lower in asymptomatic patients. CMV gB3 is the most predominant glycoprotein B genotype in Sudanese renal transplant recipients.
Synthesis and Characterization of Enaminone Derivatives of Barbituric Acid
Authors: FIRINCI Erkan 
Published: May 2019

In this study, four enaminones derived from barbituric acid were synthesized with the reaction of 5-formylbarbituric acid and selected primer amines with high yield. The structures of allcompounds were proved by using ATR-FTIR, 1H and 13C NMR spectroscopy. Also, 19F NMR spectroscopy was used for characterization of 2d. The obtained spectroscopic results have compliedwith reported similar derivatives in literature.