bluuebunny/crossref_metadata_2025_split huggingface dataset:
Magnetic viscosity effect on TEM data of an array with a fixed transmitter loop
Authors: Kozhevnikov N.O. , Antonov E.Yu. 
Published: June 2018

Abstract
In this paper, we present and discuss the results of modeling of the transient induction processes observed at different distances from the center of a transmitter loop in studies of a two-layer earth in which the magnetic susceptibility of the top layer or the base depends on frequency. Profiling graphs (the offset is plotted on the abscissa, and the EMF induced in the receiver coil at a fixed time is plotted on the ordinate) show that the polarity of the EMF changes as the offset increases. For the model with a magnetic layer of any thickness, the polarity of the EMF reverses immediately after the receiver crosses the loop wire. For the model with a magnetic base, the offset at which the EMF polarity reversal occurs is the larger the greater the thickness of the layer. For both models, the EMF at a fixed time depends on the thickness of the layer and the offset. Particularly strong dependence of the EMF on layer thickness or offset is observed near the loop side. Therefore, measurements near the wire make it possible to determine the thickness of the layer in the case where it is much less than the length of the loop side. The overall effect of magnetic relaxation and transient eddy currents leads to the fact that small changes in the layer thickness or offset can lead to a dramatic change in the transient response.
Association between ondansetron use and symptom persistence in children with concussions: A 5P substudy
Authors: Gravel Jocelyn , Boutis Kathy , Tang Ken , Beauchamp Miriam H. , Freedman Stephen B. , Dubrovsky Alexander Sasha , Gagnon Isabelle , Momoli Frank , Zemek Roger 
Published: June 2018

AbstractObjectiveOndansetron is increasingly administered to children suffering from concussion-associated nausea/vomiting. We examined the association between ondansetron administration and post-concussion symptoms in children at 1 week and 1 month following the concussion.MethodsThis was a secondary analysis of data collected prospectively in a cohort study conducted in nine pediatric emergency departments (EDs) (5P study). Participants were children ages between 5 and 17.99 years who sustained a concussion in the previous 48 hours. For the current study, only 5P participants who reported nausea and/or vomiting in the ED were eligible. The exposure of interest was ondansetron administration; the comparison group included all other participants. The primary outcome was an increase in at least three symptoms of the Post-Concussion Symptom Inventory score at 1 week and 1 month following trauma.ResultsAmong the 3,063 children included in the 5P study, 1805 (59%) reported nausea and provided data at 1 week and/or 1 month. Among them, 132 (7%) received ondansetron. Multivariable logistic regression adjusted for confounders did not show an association between ondansetron use and the risk of persistent post-concussion symptoms at 1 week (OR: 1.13 [95% CI: 0.86-1.49]), but it was associated with a higher risk at 1 month (OR: 1.33 [95% CI: 1.05-1.97]).ConclusionIn children presenting to the ED with an acute concussion, ondansetron use was associated with a higher risk of persistent post-concussion symptoms at 1 month. Although this may be related to the limitations of the design, it highlights the importance of evaluating this association using a randomized clinical trial.
Solid and fluid segments within the same molecule of stratum corneum ceramide lipid
Authors: Pham Quoc Dat , Mojumdar Enamul H. , Gooris Gert S. , Bouwstra Joke A. , Sparr Emma , Topgaard Daniel 
Published: June 2018

AbstractThe outer layer of the skin, stratum corneum (SC) is an efficient transport barrier and it tolerates mechanical deformation. At physiological conditions, the majority of SC lipids are solid, while the presence of a small amount of fluid lipids is considered crucial for SC barrier and material properties. Here we use solid-state and diffusion nuclear magnetic resonance to characterize the composition and molecular dynamics of the fluid lipid fraction in SC model lipids, focusing on the role of the essential SC lipid CER EOS, which is a ceramide esterified omega-hydroxy sphingosine linoleate with very long chain. We show that both rigid and mobile structures are present within the same CER EOS molecule, and that the linoleate segments undergo fast isotropic reorientation while exhibiting extraordinarily slow self-diffusion. The characterization of this unusual self-assembly in SC lipids provides deepened insight into the molecular arrangement in the SC extracellular lipid matrix and the role of CER EOS linoleate in the healthy and diseased skin.
Effectiveness of zinc-fortified water on zinc intake, status and morbidity in Kenyan pre-school children: a randomised controlled trial
Authors: Kujinga Prosper , Galetti Valeria , Onyango Elizabeth , Jakab Viktor , Buerkli Simone , Andang’o Pauline , Brouwer Inge D , Zimmermann Michael B , Moretti Diego 
Published: June 2018

AbstractObjectiveZn deficiency and diarrhoea are prevalent and may coexist in children living in low-resource settings. Recently, a novel approach for delivering Zn via microbiologically treated, Zn-fortified water was shown to be effective in improving Zn status in West African schoolchildren. We assessed the effectiveness of Zn-fortified, microbiologically purified water delivered as a household intervention on Zn intake, status and morbidity in children aged 2–6 years from rural western Kenya.DesignRandomised controlled trial. Intervention included households assigned to water treatment device with (ZFW) or without (FW) Zn delivery capabilitySettingRural households in Kisumu, western Kenya.SubjectsChildren aged 2–6 years.ResultsThe ZFW group had higher dietary Zn intake compared with the FW group. ZFW contributed 36 and 31 % of daily requirements for absorbable Zn in children aged 2–3 and 4–6 years, respectively, in the ZFW group. Consumption of Zn-fortified water resulted in lower prevalence of reported illness (risk ratio; 95 % CI) in the ZFW group compared with the FW group: for cold with runny nose (0·91; 0·83, 0·99; P=0·034) and abdominal pain (0·70; 0·56, 0·89; P=0·003) in the intention-to-treat analysis and for diarrhoea (0·72; 0·53, 0·96; P=0·025) in the per-protocol analysis. We did not detect an effect of treatment on plasma Zn concentration.ConclusionsDaily consumption of Zn-fortified, microbiologically treated water results in increased intake of absorbable dietary Zn and may help in preventing childhood infections in pre-school children in rural Africa.
Influence of Soil Moisture on Absorption, Translocation, and Metabolism of Florpyrauxifen-benzyl
Authors: Miller M. Ryan , Norsworthy Jason K. 
Published: June 2018

AbstractFlorpyrauxifen-benzyl is a new active ingredient that represents an additional tool in rice (Oryza sativaL.) weed control by providing an alternative mechanism of action. Studies were conducted to evaluate soil moisture influences on florpyrauxifen-benzyl absorption, translocation, and metabolism in three problematic weeds. In the absorption/translocation study, barnyardgrass [Echinochloa crus-galli(L.) P. Beauv.], hemp sesbania [Sesbania herbacea(Mill.) McVaugh], and yellow nutsedge (Cyperus esculentusL.) were treated with [14C]florpyrauxifen-benzyl under two soil moisture regimes (7.5% and 60% field capacity). Greater absorption occurred under moist conditions (60% soil moisture content). More translocation of the herbicide to the area above the treated leaf occurred under moist versus dry soil across all weed species.Sesbania herbaceatranslocated 25% of the absorbed herbicide above the treated leaf, a result greater than that of the other two weed species at 60% soil moisture. However, no differences in translocation occurred among the weed species at the 7.5% soil moisture regime. In the metabolism study, 95% of the herbicide recovered was in its acid form under the high soil moisture regime forS. herbacea, a species that shows extreme sensitivity to even low doses of this herbicide, and soil moisture influenced the amount of acid form found in all species. While these data provide a limited view into the physiological processes being affected, they do suggest that forE. crus-galli,S. herbacea, andC. esculentus, soil moisture content in the field will likely play a significant role in absorption, translocation, and metabolism of florpyrauxifen-benzyl.
Floe Size Effect on Wave‐Ice Interactions: Possible Effects, Implementation in Wave Model, and Evaluation
Authors: Boutin Guillaume , Ardhuin Fabrice , Dumont Dany , Sévigny Caroline , Girard‐Ardhuin Fanny , Accensi Mickael 
Published: June 2018

AbstractWind waves may play an important role in the evolution of sea ice. That role is largely determined by how fast the ice layer dissipates the wave energy. The transition from a continuous layer of ice to a series of broken floes is expected to have a strong impact on the several attenuation processes. Here we explore the possible effects of basal friction, scattering, and dissipation within the ice layer. The ice is treated as a single layer that can be fractured in many floes. Dissipation associated with ice flexure is evaluated using an anelastic linear dissipation and a cubic inelastic viscous dissipation. Tests aiming to reproduce a Marginal Ice Zone are used to discuss the effects of each process separately. Attenuation is exponential for friction and scattering. Scattering produces an increase in the wave height near the ice edge and broadens the wave directional spectrum, especially for short‐period waves. The nonlinear inelastic dissipation is larger for larger wave heights as long as the ice is not broken. These effects are combined in a realistic simulation of an ice break‐up event observed south of Svalbard in 2010. The recorded rapid shift from a strong attenuation to little attenuation when the ice is broken is only reproduced when using a nonlinear dissipation that vanishes when the ice is broken. A preliminary pan‐Arctic test of these different parameterizations suggests that inelastic dissipation alone is not enough and requires its combination with basal friction.
Subpolar North Atlantic Overturning and Gyre‐Scale Circulation in the Summers of 2014 and 2016
Authors: Holliday N. P. , Bacon S. , Cunningham S. A. , Gary S. F. , Karstensen J. , King B. A. , Li F. , Mcdonagh E. L. 
Published: June 2018

AbstractThe Atlantic Meridional Overturning Circulation (AMOC) is a key component of the global climate system through its transport of heat and freshwater. The subpolar North Atlantic (SPNA) is a region where the AMOC is actively developed and shaped though mixing and water mass transformation and where large amounts of heat are released to the atmosphere. Two hydrographic transbasin sections in the summers of 2014 and 2016 provide highly spatially resolved views of the SPNA velocity and property fields on a line from Canada to Greenland to Scotland. Estimates of the AMOC, isopycnal (gyre‐scale) transport, and heat and freshwater transport are derived from the observations. The overturning circulation, the maximum in northward transport integrated from the surface to seafloor and computed in density space, has a high range, with 20.6 ± 4.7 Sv in June–July 2014 and 10.6 ± 4.3 Sv in May–August 2016. In contrast, the isopycnal (gyre‐scale) circulation was lowest in summer 2014: 41.3 ± 8.2 Sv compared to 58.6 ± 7.4 Sv in 2016. The heat transport (0.39 ± 0.08 PW in summer 2014, positive is northward) was highest for the section with the highest AMOC, and the freshwater transport was largest in summer 2016 when the isopycnal circulation was high (−0.25 ± 0.08 Sv). Up to 65% of the heat and freshwater transport was carried by the isopycnal circulation, with isopycnal property transport highest in the western Labrador Sea and the eastern basins (Iceland Basin to Scotland).
A gene transfer event suggests a long-term partnership between eustigmatophyte algae and a novel lineage of endosymbiotic bacteria
Authors: Yurchenko Tatiana , Ševčíková Tereza , Přibyl Pavel , El Karkouri Khalid , Klimeš Vladimír , Amaral Raquel , Zbránková Veronika , Kim Eunsoo , Raoult Didier , Santos Lilia M A , Eliáš Marek 
Published: June 2018

Abstract
Rickettsiales are obligate intracellular bacteria originally found in metazoans, but more recently recognized as widespread endosymbionts of various protists. One genus was detected also in several green algae, but reports on rickettsialean endosymbionts in other algal groups are lacking. Here we show that several distantly related eustigmatophytes (coccoid algae belonging to Ochrophyta, Stramenopiles) are infected by Candidatus Phycorickettsia gen. nov., a new member of the family Rickettsiaceae. The genome sequence of Ca. Phycorickettsia trachydisci sp. nov., an endosymbiont of Trachydiscus minutus CCALA 838, revealed genomic features (size, GC content, number of genes) typical for other Rickettsiales, but some unusual aspects of the gene content were noted. Specifically, Phycorickettsia lacks genes for several components of the respiration chain, haem biosynthesis pathway, or c-di-GMP-based signalling. On the other hand, it uniquely harbours a six-gene operon of enigmatic function that we recently reported from plastid genomes of two distantly related eustigmatophytes and from various non-rickettsialean bacteria. Strikingly, the eustigmatophyte operon is closely related to the one from Phycorickettsia, suggesting a gene transfer event between the endosymbiont and host lineages in early eustigmatophyte evolution. We hypothesize an important role of the operon in the physiology of Phycorickettsia infection and a long-term eustigmatophyte-Phycorickettsia coexistence.
Pharmacological inactivation of the PI3K p110δ prevents breast tumour progression by targeting cancer cells and macrophages
Authors: Goulielmaki Evangelia , Bermudez-Brito Miriam , Andreou Margarita , Tzenaki Niki , Tzardi Maria , de Bree Eelco , Tsentelierou Eleftheria , Makrigiannakis Antonis , Papakonstanti Evangelia A. 
Published: June 2018

AbstractPatient selection for PI3K-targeted solid cancer treatment was based on the PIK3CA/PTEN mutational status. However, it is increasingly clear that this is not a good predictor of the response of breast cancer cells to the anti-proliferative effect of PI3K inhibitors, indicating that isoform(s) other than p110α may modulate cancer cells sensitivity to PI3K inhibition. Surprisingly, we found that although no mutations in the p110δ subunit have been detected thus far in breast cancer, the expression of p110δ becomes gradually elevated during human breast cancer progression from grade I to grade III. Moreover, pharmacological inactivation of p110δ in mice abrogated the formation of tumours and the recruitment of macrophages to tumour sites and strongly affected the survival, proliferation and apoptosis of grafted tumour cells. Pharmacological inactivation of p110δ in mice with defective macrophages or in mice with normal macrophages but grafted with p110δ-lacking tumours suppressed only partly tumour growth, indicating a requisite role of p110δ in both macrophages and cancer cells in tumour progression. Adoptive transfer of δD910A/D910A macrophages into mice with defected macrophages suppressed tumour growth, eliminated the recruitment of macrophages to tumour sites and prevented metastasis compared with mice that received WT macrophages further establishing that inactivation of p110δ in macrophage prevents tumour progression. Our work provides the first in vivo evidence for a critical role of p110δ in cancer cells and macrophages during solid tumour growth and may pave the way for the use of p110δ inhibitors in breast cancer treatment.
The transcription factor Zfp90 regulates the self-renewal and differentiation of hematopoietic stem cells
Authors: Liu Ting , Kong Wei-xia , Tang Xiao-yi , Xu Man , Wang Qing-han , Zhang Bin , Hu Liang-ding , Chen Hu 
Published: June 2018

AbstractHematopoietic stem cells (HSCs) can give rise to all blood cells that are essential to defend against pathogen invasion. The defective capability of HSC self-renewal is linked to many serious diseases, such as anemia. However, the potential mechanism regulating HSC self-renewal has not been thoroughly elucidated to date. In this study, we showed that Zfp90 was highly expressed in HSCs. Zfp90 deficiency in the hematopoietic system caused impaired HSPC pools and led to HSC dysfunction. We showed that Zfp90 deletion inhibited HSC proliferation, while HSC apoptosis was not affected. Regarding the mechanism of this effect on HSC proliferation, we found that Zfp90 interacted with Snf2l, a subunit of the NURF complex, to regulate Hoxa9 expression. Ectopic expression of Hoxa9 rescued the HSC repopulation capacity in Zfp90-deficient mice, which indicates that Hoxa9 is the downstream effector of Zfp90. In summary, our findings identify Zfp90 as a key transcription factor in determining the fate of HSCs.
Molecular characterization and integrative genomic analysis of a panel of newly established penile cancer cell lines
Authors: Zhou Qiang-hua , Deng Chuang-zhong , Li Zai-shang , Chen Jie-ping , Yao Kai , Huang Kang-bo , Liu Ting-yu , Liu Zhuo-wei , Qin Zi-ke , Zhou Fang-jian , Huang Wenlin , Han Hui , Liu Ran-yi 
Published: June 2018

AbstractCell line models are essential tools to study the molecular mechanisms underlying tumor initiation and progression. There are limited treatment options for penile squamous cell carcinoma (PSCC), accounting for 1–2% of male tumors in developing countries, and limited progress in preclinical research in PSCC due to lacking available models with identified genomic characteristics. Here, biological and molecular characteristics and whole-genomic alterations were analyzed in a panel of PSCC cell lines newly established in our laboratory. These cell lines were all human papillomavirus (HPV)-negative, epithelial-like, immortalized, and tumorigenic in nude mice, whereas they displayed different proliferation, migration and invasion capacities in vitro, and tumorigenic ability in nude mice. They were all cisplatin sensitive, anti-EGFR therapy resistant, and androgen irresponsive. Whole-genomic sequecing analysis revealed that transition mutations (C:G>T:A and T:A>C:G) were the most common substitution types in these cell lines, whereas ERCC5, TP53, PTH1, CLTCL1, NOTCH2, MAP2K3, CDK11A/B, USP6, ADCH5, BCLAF1, CDKN2A, FANCD2, HRAS, and NOTCH1 were the most frequently altered genes. Amplifications of MYC, PLAG1, NCOA2, RUNX1T1, COX6C, and EGFR and losses of FBXW7, TET2, XPC, and FANCE were frequently observed in cell lines. The exomic variations between cell lines and their corresponding cancer tissues were highly consistent. Genetic variations were mainly involved in the MAPK, Jak-STAT, TGF-beta, Notch, and apoptosis signaling pathways. Conclusively, these panel of PSCC cell lines established in our laboratory harbor some common or specific biological characteristics and genomic variations, and they may serve as optimal models to investigate the molecular mechanisms underlying the progression, metastasis, relapses, and treatment resistance of PSCC and to develop effective treatment strategy.
Inflammation, neurodegeneration and protein aggregation in the retina as ocular biomarkers for Alzheimer’s disease in the 3xTg-AD mouse model
Authors: Grimaldi Alfonso , Brighi Carlo , Peruzzi Giovanna , Ragozzino Davide , Bonanni Valentina , Limatola Cristina , Ruocco Giancarlo , Di Angelantonio Silvia 
Published: June 2018

AbstractAlzheimer's disease (AD) is the most common cause of dementia in the elderly. In the pathogenesis of AD a pivotal role is played by two neurotoxic proteins that aggregate and accumulate in the central nervous system: amyloid beta and hyper-phosphorylated tau. Accumulation of extracellular amyloid beta plaques and intracellular hyper-phosphorylated tau tangles, and consequent neuronal loss begins 10–15 years before any cognitive impairment. In addition to cognitive and behavioral deficits, sensorial abnormalities have been described in AD patients and in some AD transgenic mouse models. Retina can be considered a simple model of the brain, as some pathological changes and therapeutic strategies from the brain may be observed or applicable to the retina. Here we propose new retinal biomarkers that could anticipate the AD diagnosis and help the beginning and the follow-up of possible future treatments. We analyzed retinal tissue of triple-transgenic AD mouse model (3xTg-AD) for the presence of pathological hallmarks during disease progression. We found the presence of amyloid beta plaques, tau tangles, neurodegeneration, and astrogliosis in the retinal ganglion cell layer of 3xTg-AD mice, already at pre-symptomatic stage. Moreover, retinal microglia in pre-symptomatic mice showed a ramified, anti-inflammatory phenotype which, during disease progression, switches to a pro-inflammatory, less ramified one, becoming neurotoxic. We hypothesize retina as a window through which monitor AD-related neurodegeneration process.
Deducing the presence of proteins and proteoforms in quantitative proteomics
Authors: Bamberger Casimir , Martínez-Bartolomé Salvador , Montgomery Miranda , Pankow Sandra , Hulleman John D. , Kelly Jeffery W. , Yates John R. 
Published: June 2018

AbstractThe human genome harbors just 20,000 genes suggesting that the variety of possible protein products per gene plays a significant role in generating functional diversity. In bottom-up proteomics peptides are mapped back to proteins and proteoforms to describe a proteome; however, accurate quantitation of proteoforms is challenging due to incomplete protein sequence coverage and mapping ambiguities. Here, we demonstrate that a new software tool called ProteinClusterQuant (PCQ) can be used to deduce the presence of proteoforms that would have otherwise been missed, as exemplified in a proteomic comparison of two fly species, Drosophilamelanogaster and D. virilis. PCQ was used to identify reduced levels of serine/threonine protein kinases PKN1 and PKN4 in CFBE41o− cells compared to HBE41o− cells and to elucidate that shorter proteoforms of full-length caspase-4 and ephrin B receptor are differentially expressed. Thus, PCQ extends current analyses in quantitative proteomics and facilitates finding differentially regulated proteins and proteoforms.
High energy-density and reversibility of iron fluoride cathode enabled via an intercalation-extrusion reaction
Authors: Fan Xiulin , Hu Enyuan , Ji Xiao , Zhu Yizhou , Han Fudong , Hwang Sooyeon , Liu Jue , Bak Seongmin , Ma Zhaohui , Gao Tao , Liou Sz-Chian , Bai Jianming , Yang Xiao-Qing , Mo Yifei , Xu Kang , Su Dong , Wang Chunsheng 
Published: June 2018

AbstractIron fluoride, an intercalation-conversion cathode for lithium ion batteries, promises a high theoretical energy density of 1922 Wh kg–1. However, poor electrochemical reversibility due to repeated breaking/reformation of metal fluoride bonds poses a grand challenge for its practical application. Here we report that both a high reversibility over 1000 cycles and a high capacity of 420 mAh g−1 can be realized by concerted doping of cobalt and oxygen into iron fluoride. In the doped nanorods, an energy density of ~1000 Wh kg−1 with a decay rate of 0.03% per cycle is achieved. The anion’s and cation’s co-substitutions thermodynamically reduce conversion reaction potential and shift the reaction from less-reversible intercalation-conversion reaction in iron fluoride to a highly reversible intercalation-extrusion reaction in doped material. The co-substitution strategy to tune the thermodynamic features of the reactions could be extended to other high energy conversion materials for improved performance.