bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nats’eji (healing): Examining patient and provider experiences with hospital-based Indigenous wellness services in Northwest Territories, Canada
Authors: Roher Sophie Isabelle Grace , Andrew Paul , Chatwood Susan , Fairman Kimberly , Galloway Tracey , Mashford-Pringle Angela , Gibson Jennifer L. 
Published: November 2024

Abstract
Objective
This study aimed to examine how Indigenous patients and biomedical healthcare providers understand and experience the Indigenous wellness services at a hospital in the Northwest Territories.

Methods
The qualitative study (May 2018–June 2022) was overseen by a regional Indigenous Community Advisory Committee. Guided by Two-Eyed Seeing and post-colonial theory, the study employed a community-engaged research design, and included two strategies for data generation: (1) interviews with Indigenous Elders, patient advocates, biomedical healthcare providers, policy makers, and hospital administrators (n = 41), and (2) iterative sharing circles with Indigenous Elders (n = 4). Data from the interviews and first sharing circle were transcribed, thematically analyzed, and presented to the sharing circle Elders for validation.

Results
The study revealed three overarching and related themes: (1) Elders and patient advocates emphasized that while the Indigenous wellness services at the hospital play a pivotal role connecting patients with cultural supports, the hospital was still not effectively bringing Indigenous healing practices into hospital care; (2) participants identified that structural factors (i.e., policy and governance decisions) shaped patients’ experiences with the wellness services; and (3) participants underscored that deeply rooted forces (i.e., racism, colonialism, and biomedical dominance) inhibit the integration of Indigenous healing practices.

Conclusion
The findings extend understandings of hospital-based Indigenous wellness services by surfacing relationships between deeply rooted forces, organizational structures, and Indigenous patients’ experiences. Altogether, they suggest that to advance care for Indigenous patients and improve the integration of Indigenous healing practices, a system-wide transformation is necessary, which includes Indigenous governance at the hospital and a recognition of the value of Indigenous healing practices.
Predictors of New-Onset Atrial Fibrillation in Patients With Chronic Coronary Artery Disease After Coronary Artery Bypass Grafting: a Prospective, Observational, Single-Centre, Non-Randomized Study
Authors: Sokolova N. Yu. , Savelyeva E. A. , Martynova K. A. , Makhauri A. D. , Medzhidov S. R. 
Published: November 2024

Aim. To study the factors that influence the occurrence of postoperative atrial fibrillation (POAF) in patients with chronic ischemic heart disease (IHD) after coronary artery bypass grafting (CABG).Material and methods. This single-center prospective observational non-randomized study included 152 patients with chronic IHD. Mean age of patients was 64.4±5.9 years. All patients after CABG were divided into two groups based on the occurrence of atrial fibrillation (AF) in the early postoperative period: group 1, with POAF (n=43; 28.3%) and group 2, without POAF (n=109; 71.7%). The primary study endpoint was new-onset POAF in the early postoperative (hospital) period after CABG. The secondary study endpoint was in-hospital postoperative complications (non-fatal/fatal acute coronary syndrome (ACS), non-fatal/fatal stroke, major bleeding, death).Results. Patients with POAF had significantly more pronounced structural and functional changes in the heart than patients with preserved sinus rhythm after CABG: larger left ventricular (LV) volume, greater LV myocardial mass, lower LV systolic function parameters and impaired diastolic function, and an enlarged left atrial (LA) cavity. Analysis of in-hospital complications did not show any differences between the groups associated with the development of POAF. The following risk factors for POAF were identified: age older than 65 years (p=0.022), body mass index ≥30.5 kg/m2 (p=0.020), epicardial adipose tissue thickness >10.5 mm (p=0.015), indexed LA volume >33 ml/m2 (p<0.001), LV myocardial mass index >115 g/m2 (p=0.042), left main coronary artery disease >50% (p=0.043), duration of cardiopulmonary bypass during CABG >60 min (p=0.019), blood potassium concentration in the early postoperative period after CABG (on the first day) <3.6 mmol/l (p<0.001), and pericardial effusion volume in the early postoperative period >88 ml (p<0.001).Conclusion. Determining the risk of developing POAF is important and necessary for the closest monitoring of a patient with chronic IHD in the postoperative period.
4‑Year Experience of the Cardio-Oncology Center of Sechenov University: Single-Center Epidemiological Study
Authors: Kirichenko Yu. Yu. , Ilgisonis I. S. , Chernichkina A. D. , Palienko A. V. , Buduscheva O. I. , Pakhtusov N. N. , Khabarova N. V. , Belenkov Yu. N. 
Published: November 2024

Aim. To present the four-year experience and the accomplishments of the Scientific and Practical Cardio-Oncology Center of the Sechenov University.Material and methods. The records of patients referred for cardio-oncology consultation from January 2020 through March 2024 were retrospectively analyzed. The patients’ cardiovascular (CV) status was assessed at baseline and after optimizing the cardiac therapy during the antitumor treatment. The endpoints were the completion of all antitumor therapy courses and the level of overall and CV mortality.Results. Among 233 enrolled patients (66% women), a considerable part belonged to the group of high/very high cardio-oncological risk (n=134, 57%). Various cardiovascular toxicities were observed in 22% of patients. At baseline, these patients significantly more frequently had heart failure and ischemic heart disease as well as previous radiation and chemotherapy. After the optimization of cardiac therapy, 88% of patients successfully completed all scheduled treatments. The overall mortality, including the CV mortality, was 14% (n=7).Conclusion. Creation of cardio-oncological services allows considerably reducing the probability of adverse CV events during the antitumor therapy and successfully completing all scheduled treatments in most patients..
Flavor Analysis and Strain Exploitation of Fermented Rice Noodles
Authors: Chen Di , Gu Zhengbiao , Li Zhaofeng , Hong Yan , Cheng Li , Li Caiming 
Published: November 2024

<p align="justify">Fermented rice noodles is popular for its unique flavor, but the study on flavor is lacking, especially the screening of corresponding strains. For the first time, the relationship of microorganism and flavor was unveiled, based on the research of a typical fermented rice noodles from Guilin in China. Flavors were dissected by HPLC-MS, GC-MS, GC-MS-O (Gas chromatography-mass and spectrometry-olfactometry) analyses, while microbial community was investigated by amplicon sequencing and metagenomics, afterwards microorganisms and flavors were connected via correlation analysis and function annotation, hen strains could be explored based on the analyses. Ethanol, 1-butanol,3-methyl-, 2-butanone, 3-hydroxy-, acetic acid and phenylethanol were discovered to be key flavors, biogenic amines were not abundant enough to have bad effect on flavor, but putrescine, tyramine and histamine produced in fermentation needed supervision for safety. Analysis indicated that fermentation was dominated by lactic acid bacteria. Further analysis revealed that Lactobacillus, Acetobacter and Lactococcus were primary creators for these key flavors. In particular, Lactobacillus showed great potential in flavor forming. Additionally, later experiment suggested that samples fermented by L. fermentum and L. plantarum (both belong to Lactobacillus) possessed great flavors, when L. fermentum also showed the ability to inhibit mold and reduce biogenic amine content.</p>
Biocontrol Efficacy of Trichoderma and Bacillus Isolates against Sclerotium rolfsii under in vitro Conditions
Authors: Vamshi J. , Devi G. Uma , Somraj B. , maheswari T. Uma , Supriya K. , Sudini Hari Kishan 
Published: November 2024

Background: Sclerotium rolfsii is a polyphagous pathogen which is uneconomical and difficult to control due to its broad host range and persistence due to its tough resting structures, i.e., sclerotia. Chemical control has been supplanted by biological phytopathogen control. Antibiotic microorganisms are regarded as one of the viable management techniques in an integrated approach for ecofriendly management. Methods: Twenty three Trichoderma spp. and twenty one Bacillus species were isolated at Groundnut Pathology Laboratory, ICRISAT, Patancheru from rhizospheric soils of Telangana during rabi 2022-23 and 2023-24 and examined for antagonistic activity against Sclerotium rolfsii in an effort to design an effective biocontrol system for the management of Sclerotium rolfsii in groundnut. Result: The highest antagonistic activity against Sclerotium rolfsii has been observed in Trichoderma viride and Bacillus cereus. In the dual culture assay, Trichoderma viride and Bacillus cereus inhibited Sclerotium rolfsii by up to 65.33 per cent and 58.22 per cent, respectively. In the metabolites assay, Trichoderma viride and Bacillus cereus inhibited Sclerotium rolfsii by 63.33 per cent and 55.40 per cent, respectively. Thus, Trichoderma viride and Bacillus cereus displayed promising biofungicidal capabilities against the stem rot pathogen. ​
A Randomized Controlled Trial on the Effect of Zucchini Seed Oil on Nipple Pain Among Lactating Women
Authors: Saffari Mehri , Ghorbani Mohammad , Mahmoudian Azamsadat , Khajavian Nasim , Bahri Narjes 
Published: November 2024

Objective:Nipple pain is one of the most common reasons for early termination of breastfeeding; thus, effective treatment of this condition is very important. Previous studies have proposed the anti-inflammatory and analgesic properties of theCucurbita pepoplant. The present study was conducted to determine the effects ofC. peposeed oil on the severity of nipple pain in lactating women.Methods:In this randomized clinical trial study, 60 lactating women with nipple pain were randomly assigned to either the intervention group (zucchini seed oil) or the control group (breast milk). In the intervention group, 0.5 mL of zucchini seed oil was applied to the nipples and areola three times a day after each breastfeeding session to ensure the absence of an allergy to zucchini compounds. In the control group, three to four drops of breast milk were applied to the nipples and areola after each breastfeeding session. Pain score was recorded using a visual analog scale before the intervention and on the third and eighth day after the intervention. Data analysis was done using the Statistical Software for Social Sciences software (version 24). Apvalue of less than .05 was considered statistically significant.Results:Before the intervention, there was no significant difference between the intervention and control groups with regard to mean pain severity (p= .330). On the third day, the mean pain intensity in the intervention group significantly decreased compared with the control group (p= .023). On the eighth day, the mean pain severity was significantly lower in the intervention group compared with the control group (p= .016).Conclusions:The findings of this study indicate that zucchini seed oil extract was effective in reducing the severity of nipple pain in lactating women. Therefore, use of this oil extract is suggested for treatment of nipple pain in lactating women.
Numerical Investigation and Design Optimization of Centrifugal Water Pump with Splitter Blades Using Response Surface Method
Authors: Abuan Justin , Honra Jaime 
Published: November 2024

The use of centrifugal pumps for water supply and distribution applications is evident in every industry as they efficiently transport fluid from a certain point. However, the sector faces severe concerns regarding its operating efficiency due to accumulated losses and sudden power consumption growth. Thus, it is crucial to find a remedy to mitigate these concerns and improve the performance of the centrifugal pump. This study aims to provide an improved design of centrifugal water pumps by analyzing the effects of changing its design parameters. Splitter blades, with a length of 80% of the main blade, will be included in the design. Using ANSYS software to generate a baseline design model, CFD analysis will be conducted. Then, an optimization study will be performed using the Response Surface Method (RSM) to formulate an optimized design. Results indicate that finding a perfect balance between the placement of the splitter blades, the design of the volute tongue clearance and thickness, and configuring the ellipse ratio of the splitter blades improves the pump&#039;s performance. The optimal design results in 27.35%, 15.70%, 28.18%, 16.67%, and 8.36% improvement in total efficiency, total head, static efficiency, static head, and power consumption, respectively.
Distal Nerve Transfers for Restoration of Finger Flexion and Extension in Klumpke Birth Palsy
Authors: Chim Harvey 
Published: November 2024

Case:
Traditional surgical intervention for brachial plexus birth injury (BPBI) has involved proximal nerve grafting. At the age of 6 months, an infant with Klumpke birth palsy had not recovered finger flexion and extension. He underwent extensor carpi radialis brevis to anterior interosseous nerve and supinator-to-posterior interosseous nerve transfers. Three months postoperatively, he was noted to have recovery of finger flexion and extension which continued to improve to 18 months postoperative follow-up.

Conclusion:
This case shows effectiveness of early surgical intervention using distal nerve transfers, as opposed to traditional proximal nerve grafting, for reanimating the hand in BPBI.
Efficacy of Liposome-Encapsulated Vancomycin Against Methicillin-Resistant Staphylococcus aureus
Authors: Erdene Enkhtaivan , Munkhjargal Odonchimeg , Dorjbal Enkhjargal , Oidov Baatarkhuu , Byambaa Ariunsanaa 
Published: November 2024

Abstract

Bacterial infections significantly contribute to global morbidity and mortality, with antibiotic-resistant strains, such as Methicillin-resistant Staphylococcus aureus (MRSA), presenting severe treatment challenges. This study investigates the potential of liposome-encapsulated vancomycin as a novel treatment to combat antibiotic resistance. Phospholipids were extracted from egg yolk, and liposomes were prepared using the freeze-thaw method. The liposome-encapsulated vancomycin formulation was characterized using infrared spectroscopy, atomic force microscopy (AFM), and high-performance liquid chromatography (HPLC). Infrared spectroscopy confirmed the structural integrity and purity of the phospholipids, while AFM revealed a uniform liposome size, with an average diameter of 157 nm and a polydispersity index (PDI) of 0.0442, indicating high stability and suitability for drug delivery. The antibacterial efficacy of liposome-encapsulated vancomycin was tested against MRSA and Staphylococcus aureus, demonstrating that the encapsulated vancomycin inhibited bacterial growth at doses significantly lower than free vancomycin. Liposome-encapsulated vancomycin achieved 100% inhibition of MRSA and S. aureus at higher dilutions, while free vancomycin was only partially effective. These findings highlight the enhanced potential of liposome-based drug delivery in reducing bacterial load and overcoming antibiotic resistance. The study emphasizes the promise of nanotechnology in improving antibiotic efficacy, offering a potential solution to the global health crisis posed by antibiotic-resistant pathogens.
Men’s views on factors contributing to their poor health-seeking behavior in Limpopo Province, South Africa
Authors: Chavalala Lazarros , Lebese Rachele Tsakane , Makhado Lufuno 
Published: November 2024

Abstract

Background
The reasons for men not to seek healthcare seem similar across the world. They avoid going for regular medical check-ups, and preventive care and often disregard symptoms or delay seeking medical attention when sick, in pain, or even when their lives are in danger.
Methods
This study sought to explore the views of men on factors contributing to poor health-seeking behavior among men in Mopani, Vhembe, and Capricorn district municipalities. A qualitative descriptive design was used and individual semi-structured interviews were used to collect data in May and June 2024 from 21 men who were purposively selected. Collected data were audio recorded and transcribed verbatim for analysis. Tech’s eight steps were used to analyze data and guide the development of emerged main themes and sub-themes. Trustworthiness was ensured through credibility, confirmability, dependability, and transferability. Ethical approval was obtained from the University of Venda research ethics committee with Ethics Approval Number: FHS/21/PH/26/1215
Results
Self-medication, fear of knowing own health status, peer and community elders influence, stigma and masculinity beliefs were among the reasons participants avoided utilizing health services. Educational and health promotional campaigns, challenging societal norms, and employing more nurses were suggested as strategies to improve men’s health-seeking behavior.
Conclusions:
It is evident from the findings that there are several factors that act as barriers for men to seek health care services at public health facilities. There is a need to challenge societal views of men and masculinity beliefs to improve men’s health-seeking behavior.
Dengue epidemiology and molecular identification of vector mosquitoes (Aedes sp.) in Mizoram, Northeast India
Authors: Ramliana Van , Vanlalawmpuii Ralte , Rosangkima Gabriel , Pautu Lalfakzuala , Ropuia Hun , Kumar Nachimuthu S. , Lalthanzara Hmar 
Published: November 2024

Abstract

Background
Dengue is the most important arboviral disease, and Aedes aegypti and Aedes albopictus are the major vectors. It is one of the most recent emerging diseases in Mizoram state with a fast-growing burden over the years. The present study aimed to explore the epidemiological patterns of dengue in Mizoram, and to identify vector mosquito species. Methods
In the present study, two years secondary data of dengue disease in Mizoram was studied. Dengue vector mosquitoes and dengue virus serotypes circulating in vector mosquitoes in Aizawl City were identified by real-time RT-PCR and C-prM gene sequence analysis.
Results
In Mizoram State, the annual case prevalence of dengue was found to be 300.45 per 100,000 persons. Seasonality of the disease was also noted, with the peak season occurring from July to December. There is a significant positive relationship between age and the disease prevalence. DENV was detected in 10 pools of Ae. aegypti with a minimum infection rate (MIR) of 23.04, and 8 pools of Ae. albopictus with a minimum infection rate of 18.51. The viral isolates were found to correspond to serotypes 2 (DENV2) and 3 (DENV3). DENV2 serotype was more common, and co-infection with DENV2 and DENV3 was also detected in one locality.
Conclusions
There is an increasing trend in dengue infection in Mizoram, and seasonality of the disease was observed with the peak season during July to December. The higher incidence rate among the adults indicates that outdoor activities could be related to the high risk of the infection. Early identification and frequent monitoring of infected vector mosquitoes will yield an early warning indicator for forecasting dengue epidemics in the future.
Advanced pharmacological and pharmacokinetic evaluation of 1,3 dimethylpurine-2,6-dione derivative (GR-14) with prominent mood modulating activity in rats
Authors: Cios Agnieszka , Chłoń-Rzepa Grażyna , Jastrzębska-Więsek Magdalena , Pociecha Krzysztof , Wójcik-Pszczoła Katarzyna , Pękala Elżbieta , Wesołowska Anna 
Published: November 2024

Abstract

Background Research on new candidates for antidepressant/anxiolytic drugs from the group of long-chain arylpiperazines (LCAPs) containing a 1,3-dimethylpurine-2,6-dione as a terminal amide fragment fits into modern exploration trend. This study aimed to examine, for the first time in rats, pharmacodynamic (antidepressant- and anxiolytic-like) and pharmacokinetic properties of 7-(5-(4-(3-chlorophenyl)piperazin-1-yl)pentyl)-1,3-dimethyl-3,7-dihydro-1H-purine-2,6-dione hydrochloride (GR-14).
Methods Antidepressant- and anxiolytic-like activities have been conducted in the forced swim (FST) and Vogel conflict drinking tests, respectively. Pharmacokinetic characteristic of GR-14, distribution into rat tissues as well as several in vitro ADME-Tox parameters (hepatocytotoxic, neurocytotoxic, metabolic stability) have been defined.
Results GR-14 produces strong and dose-dependent antidepressant- and anxiolytic-like effects in both tests used. Pharmacokinetic findings demonstrate that GR-14 reveals linear pharmacokinetics tested after intravenous (iv) and was rapidly absorbed after oral (po) administration. It rapidly crosses the blood-brain barrier (BBB) which is vital for therapeutic effects in vivo in psychiatric diseases, depression and anxiety. Moreover, it is slowly eliminated from the brain, maintaining concentrations higher than those in plasma at the last time point measured. Further studies have also shown that GR-14 is characterized as average high clearance drug in rat liver microsomes ​​and exerts neither hepatocytotoxic or neurocytotoxic effects in vitro.
Conclusion The tested derivative GR-14 presents prominent mood modulating activity in rats, has promising pharmacokinetic parameters and a good safety profile. The beneficial pharmacology and pharmacokinetics of GR-14 in vivo are in a high concordance with its profile in vitro, thus underlining very hopeful properties to support the early development process.
Multimodal tumor suppression by METTL3 gene knockdown in melanoma and colon cancer cells
Authors: Bazargani Arezoo , Taha Masoumeh Fakhr , Soltani Bahram Mohammad , Javeri Arash 
Published: November 2024

Abstract

The m6A methyltransferase METTL3 is involved in regulating mRNA biogenesis, degradation and translation via N6-methyladenosine (m6A) modification. Altered m6A homeostasis is linked to development, progression, invasion and metastasis of some cancers. In the current study, we aimed to investigate the impact of METTL3 knockdown by shRNA on proliferation and invasiveness of human colorectal and melanoma cancer cell lines. A specific shRNA against METTL3 mRNA was designed and inserted into an expression vector. Highly invasive colorectal cancer cell line SW480 and melanoma cell line A375 were cultured and transfected by METTL3-shRNA and scramble-control vectors and kept under culture condition for two weeks. The cells were harvested for analysis of gene expression by qPCR, invasion assay using 3D spheroid assay and cell cycle and apoptosis analyses. In the METTL3-shRNA transfected cells, the expression of METTL3, VIM, SNAI1, SNAI2, ZEB1, CDH1 and TGFB1 genes were downregulated significantly compared to the scramble-control transfected cells. Expression of b-catenin, N-cadherin, vimentin, ZEB1, pro- and active MMP2, OCT4A, SOX2 and MYC proteins were also downregulated following METTL3 knockdown. Transfection by METTL3-shRNA reduced proliferation rate of the cells and increased the apoptotic rate significantly. Both migration and invasion rate of the cancer cells transfected with METTL3-shRNA were significantly decreased. These results have shown a pro-oncogenic role for METTL3 in colorectal and melanoma cancer cells and provided evidence that METTL3 inhibition may serve as a common approach for tumor suppression among different types of cancer although this requires further investigation.