bluuebunny/crossref_metadata_2025_split huggingface dataset:
LINC complex-Lis1 interplay controls MT1-MMP matrix digest-on-demand response for confined tumor cell migration
Authors: Infante Elvira , Castagnino Alessia , Ferrari Robin , Monteiro Pedro , Agüera-González Sonia , Paul-Gilloteaux Perrine , Domingues Mélanie J. , Maiuri Paolo , Raab Matthew , Shanahan Catherine M. , Baffet Alexandre , Piel Matthieu , Gomes Edgar R. , Chavrier Philippe 
Published: June 2018

AbstractCancer cells’ ability to migrate through constricting pores in the tissue matrix is limited by nuclear stiffness. MT1-MMP contributes to metastasis by widening matrix pores, facilitating confined migration. Here, we show that modulation of matrix pore size or of lamin A expression known to modulate nuclear stiffness directly impinges on levels of MT1-MMP-mediated pericellular collagenolysis by cancer cells. A component of this adaptive response is the centrosome-centered distribution of MT1-MMP intracellular storage compartments ahead of the nucleus. We further show that this response, including invadopodia formation in association with confining matrix fibrils, requires an intact connection between the nucleus and the centrosome via the linker of nucleoskeleton and cytoskeleton (LINC) complex protein nesprin-2 and dynein adaptor Lis1. Our results uncover a digest-on-demand strategy for nuclear translocation through constricted spaces whereby confined migration triggers polarization of MT1-MMP storage compartments and matrix proteolysis in front of the nucleus depending on nucleus-microtubule linkage.
Information Spread and Topic Diffusion in Heterogeneous Information Networks
Authors: Molaei Soheila , Babaei Sama , Salehi Mostafa , Jalili Mahdi 
Published: June 2018

AbstractDiffusion of information in complex networks largely depends on the network structure. Recent studies have mainly addressed information diffusion in homogeneous networks where there is only a single type of nodes and edges. However, some real-world networks consist of heterogeneous types of nodes and edges. In this manuscript, we model information diffusion in heterogeneous information networks, and use interactions of different meta-paths to predict the diffusion process. A meta-path is a path between nodes across different layers of a heterogeneous network. As its most important feature the proposed method is capable of determining the influence of all meta-paths on the diffusion process. A conditional probability is used assuming interdependent relations between the nodes to calculate the activation probability of each node. As independent cascade models, we consider linear threshold and independent cascade models. Applying the proposed method on two real heterogeneous networks reveals its effectiveness and superior performance over state-of-the-art methods.
Quantitative assessment of individual populations within polymicrobial biofilms
Authors: Lopes Susana Patrícia , Azevedo Nuno Filipe , Pereira Maria Olívia 
Published: June 2018

AbstractSelecting appropriate tools providing reliable quantitative measures of individual populations in biofilms is critical as we now recognize their true polymicrobial and heterogeneous nature. Here, plate count, quantitative real-time polymerase chain reaction (q-PCR) and peptide nucleic acid probe-fluorescence in situ hybridization (PNA-FISH) were employed to quantitate cystic fibrosis multispecies biofilms. Growth of Pseudomonas aeruginosa, Inquilinus limosus and Dolosigranulum pigrum was assessed in dual- and triple-species consortia under oxygen and antibiotic stress. Quantification methods, that were previously optimized and validated in planktonic consortia, were not always in agreement when applied in multispecies biofilms. Discrepancies in culture and molecular outcomes were observed, particularly for triple-species consortia and antibiotic-stressed biofilms. Some differences were observed, such as the higher bacterial counts obtained by q-PCR and/or PNA-FISH (≤4 log10 cells/cm2) compared to culture. But the discrepancies between PNA-FISH and q-PCR data (eg D. pigrum limited assessment by q-PCR) demonstrate the effect of biofilm heterogeneity in method’s reliability. As the heterogeneity in biofilms is a reflection of a myriad of variables, tailoring an accurate picture of communities´ changes is crucial. This work demonstrates that at least two, but preferentially three, quantification techniques are required to obtain reliable measures and take comprehensive analysis of polymicrobial biofilm-associated infections.
Experimental evidence that thermal selection shapes mitochondrial genome evolution
Authors: Lajbner Zdeněk , Pnini Reuven , Camus M. Florencia , Miller Jonathan , Dowling Damian K. 
Published: June 2018

AbstractMitochondria are essential organelles, found within eukaryotic cells, which contain their own DNA. Mitochondrial DNA (mtDNA) has traditionally been used in population genetic and biogeographic studies as a maternally-inherited and evolutionary-neutral genetic marker. However, it is now clear that polymorphisms within the mtDNA sequence are routinely non-neutral, and furthermore several studies have suggested that such mtDNA polymorphisms are also sensitive to thermal selection. These observations led to the formulation of the “mitochondrial climatic adaptation” hypothesis, for which all published evidence to date is correlational. Here, we use laboratory-based experimental evolution in the fruit fly, Drosophila melanogaster, to test whether thermal selection can shift population frequencies of two mtDNA haplogroups whose natural frequencies exhibit clinal associations with latitude along the Australian east-coast. We present experimental evidence that the thermal regime in which the laboratory populations were maintained drove changes in haplogroup frequencies across generations. Our results strengthen the emerging view that intra-specific mtDNA variants are sensitive to selection, and suggest spatial distributions of mtDNA variants in natural populations of metazoans might reflect adaptation to climatic environments rather than within-population coalescence and diffusion of selectively-neutral haplotypes across populations.
Quantum walks on regular uniform hypergraphs
Authors: Liu Ying , Yuan Jiabin , Duan Bojia , Li Dan 
Published: June 2018

AbstractQuantum walks on graphs have shown prioritized benefits and applications in wide areas. In some scenarios, however, it may be more natural and accurate to mandate high-order relationships for hypergraphs, due to the density of information stored inherently. Therefore, we can explore the potential of quantum walks on hypergraphs. In this paper, by presenting the one-to-one correspondence between regular uniform hypergraphs and bipartite graphs, we construct a model for quantum walks on bipartite graphs of regular uniform hypergraphs with Szegedy’s quantum walks, which gives rise to a quadratic speed-up. Furthermore, we deliver spectral properties of the transition matrix, given that the cardinalities of the two disjoint sets are different in the bipartite graph. Our model provides the foundation for building quantum algorithms on the strength of quantum walks on hypergraphs, such as quantum walks search, quantized Google’s PageRank, and quantum machine learning.
Environmental drivers of spatiotemporal foraging intensity in fruit bats and implications for Hendra virus ecology
Authors: Giles John R. , Eby Peggy , Parry Hazel , Peel Alison J. , Plowright Raina K. , Westcott David A. , McCallum Hamish 
Published: June 2018

AbstractIn the Australian subtropics, flying-foxes (family Pteropididae) play a fundamental ecological role as forest pollinators. Flying-foxes are also reservoirs of the fatal zoonosis, Hendra virus. Understanding flying fox foraging ecology, particularly in agricultural areas during winter, is critical to determine their role in transmitting Hendra virus to horses and humans. We developed a spatiotemporal model of flying-fox foraging intensity based on foraging patterns of 37 grey-headed flying-foxes (Pteropus poliocephalus) using GPS tracking devices and boosted regression trees. We validated the model with independent population counts and summarized temporal patterns in terms of spatial resource concentration. We found that spatial resource concentration was highest in late-summer and lowest in winter, with lowest values in winter 2011, the same year an unprecedented cluster of spillover events occurred in Queensland and New South Wales. Spatial resource concentration was positively correlated with El Niño Southern Oscillation at 3–8 month time lags. Based on shared foraging traits with the primary reservoir of Hendra virus (Pteropus alecto), we used our results to develop hypotheses on how regional climatic history, eucalypt phenology, and foraging behaviour may contribute to the predominance of winter spillovers, and how these phenomena connote foraging habitat conservation as a public health intervention.
Pyruvate dehydrogenase complex plays a central role in brown adipocyte energy expenditure and fuel utilization during short-term beta-adrenergic activation
Authors: Held Ntsiki M. , Kuipers Eline N. , van Weeghel Michel , van Klinken Jan Bert , Denis Simone W. , Lombès Marc , Wanders Ronald J. , Vaz Frédéric M. , Rensen Patrick C. N. , Verhoeven Arthur J. , Boon Mariëtte R. , Houtkooper Riekelt H. 
Published: June 2018

AbstractActivation of brown adipose tissue (BAT) contributes to total body energy expenditure through energy dissipation as heat. Activated BAT increases the clearance of lipids and glucose from the circulation, but how BAT accommodates large influx of multiple substrates is not well defined. The purpose of this work was to assess the metabolic fluxes in brown adipocytes during β3-adrenergic receptor (β3-AR) activation.T37i murine preadipocytes were differentiated into brown adipocytes and we used Seahorse respirometry employing a set of specific substrate inhibitors in the presence or absence of β3-AR agonist CL316,243. The main substrate used by these brown adipocytes were fatty acids, which were oxidized equally during activation as well as during resting condition. [U-13C]-glucose tracer-based metabolomics revealed that the flux through the TCA cycle was enhanced and regulated by pyruvate dehydrogenase (PDH) activity. Based on 13C-tracer incorporation in lipids, it appeared that most glucose was oxidized via TCA cycle activity, while some was utilized for glycerol-3-phosphate synthesis to replenish the triglyceride pool. Collectively, we show that while fatty acids are the main substrates for oxidation, glucose is also oxidized to meet the increased energy demand during short term β3-AR activation. PDH plays an important role in directing glucose carbons towards oxidation.
Temporal window for detection of inflammatory disease using dynamic cell tracking with time-lapse MRI
Authors: Masthoff Max , Gran Sandra , Zhang Xueli , Wachsmuth Lydia , Bietenbeck Michael , Helfen Anne , Heindel Walter , Sorokin Lydia , Roth Johannes , Eisenblätter Michel , Wildgruber Moritz , Faber Cornelius 
Published: June 2018

AbstractTime-lapse MRI was implemented for dynamic non-invasive cell tracking of individual slowly moving intravascular immune cells. Repetitive MRI acquisition enabled dynamic observation of iron oxide nanoparticle (ION) labelled cells. Simulations of MRI contrast indicated that only cells moving slower than 1 µm/s were detectable. Time-lapse MRI of the brain was performed after either IONs or ION-labelled monocytes were injected intravenously into naïve and experimental autoimmune encephalomyelitis (EAE) bearing mice at a presymptomatic or symptomatic stage. EAE mice showed a reduced number of slow moving, i.e. patrolling cells before and after onset of symptoms as compared to naïve controls. This observation is consistent with the notion of altered cell dynamics, i.e. higher velocities of immune cells rolling along the endothelium in the inflamed condition. Thus, time-lapse MRI enables for assessing immune cell dynamics non-invasively in deep tissue and may serve as a tool for detection or monitoring of an inflammatory response.
Cooperative bi-exponential decay of dye emission coupled via plasmons
Authors: Lyvers David P. , Moazzezi Mojtaba , de Silva Vashista C. , Brown Dean P. , Urbas Augustine M. , Rostovtsev Yuri V. , Drachev Vladimir P. 
Published: June 2018

AbstractBi-exponential decay of dye fluorescence near the surface of plasmonic metamaterials and core-shell nanoparticles is shown to be an intrinsic property of the coupled system. Indeed, the Dicke, cooperative states involve two groups of transitions: super-radiant, from the most excited to the ground states and sub-radiant, which cannot reach the ground state. The relaxation in the sub-radiant system occurs mainly due to the interaction with the plasmon modes. Our theory shows that the relaxation leads to the population of the sub-radiant states by dephasing the super-radiant Dicke states giving rise to the bi-exponential decay in agreement with the experiments. We use a set of metamaterial samples consisting of gratings of paired silver nanostrips coated with Rh800 dye molecules, having resonances in the same spectral range. The bi-exponential decay is demonstrated for Au\SiO2\ATTO655 core-shell nanoparticles as well, which persists even when averaging over a broad range of the coupling parameter.
LIM Protein Ajuba associates with the RPA complex through direct cell cycle-dependent interaction with the RPA70 subunit
Authors: Fowler Sandy , Maguin Pascal , Kalan Sampada , Loayza Diego 
Published: June 2018

AbstractDNA damage response pathways are essential for genome stability and cell survival. Specifically, the ATR kinase is activated by DNA replication stress. An early event in this activation is the recruitment and phosphorylation of RPA, a single stranded DNA binding complex composed of three subunits, RPA70, RPA32 and RPA14. We have previously shown that the LIM protein Ajuba associates with RPA, and that depletion of Ajuba leads to potent activation of ATR. In this study, we provide evidence that the Ajuba-RPA interaction occurs through direct protein contact with RPA70, and that their association is cell cycle-regulated and is reduced upon DNA replication stress. We propose a model in which Ajuba negatively regulates the ATR pathway by directly interacting with RPA70, thereby preventing inappropriate ATR activation. Our results provide a framework to further our understanding of the mechanism of ATR regulation in human cells in the context of cellular transformation.
Quantitative proteomic analysis using iTRAQ to identify salt-responsive proteins during the germination stage of two Medicago species
Authors: Long Ruicai , Gao Yanli , Sun Hao , Zhang Tiejun , Li Xiao , Li Mingna , Sun Yan , Kang Junmei , Wang Zhen , Ding Wang , Yang Qingchuan 
Published: June 2018

AbstractSalt stress is one of the primary abiotic stresses responsible for decreasing crop yields worldwide. Germinating seeds can be greatly influenced by saline conditions. In this study, the physiological and phenotypic changes induced by salt treatments (10–50 mM NaCl and Na2SO4 mixtures) were analysed for Zhongmu-3 (Medicago sativa) and R108 (Medicago truncatula) seedlings. Our observations indicated that Zhongmu-3 was more salt-tolerant than R108. To characterize the protein expression profiles of these two Medicago species in response to salt stress, an iTRAQ-based quantitative proteomic analysis was applied to examine salt-responsive proteins. We identified 254 differentially changed salt-responsive proteins. Compared with control levels, the abundance of 121 proteins increased and 44 proteins decreased in salt-treated Zhongmu-3 seedlings, while 119 proteins increased and 18 proteins decreased in R108 seedlings. Moreover, 48 differentially changed proteins were common to Zhongmu-3 and R108 seedlings. A subsequent functional annotation indicated these proteins influenced diverse processes, such as catalytic activity, binding, and antioxidant activity. Furthermore, the corresponding transcript levels of 15 differentially changed proteins were quantified by qRT-PCR. The data presented herein provide new insights into salt-responsive proteins, with potential implications for enhancing the salt tolerance of Medicago species.
Deutschlandweites Vergiftungsspektrum im Kindes- und
Jugendalter
Authors: Geith Stefanie , Ganzert Martin , Schmoll Sabrina , Acquarone Daniela , Deters Michael , Sauer Oliver , Stürer Andreas , Tutdibi Erol , Wagner Rafael , Eyer Florian 
Published: June 2018

Zusammenfassung
Hintergrund Vergiftungen verursachen bei Kindern den Großteil der
Notfalleinsätze in Deutschland, die durch präventive Maßnahmen verhindert
oder gemildert werden könnten. Daher ist für den Pädiater das Wissen um
häufige Intoxikationen essentiell. Die vorliegende Arbeit zeigt allgemeine
und epidemiologische Daten zu Vergiftungen sowie einen Überblick über die
häufigsten einzelnen Noxen und -kategorien im Kindes- und Jugendalter.
Methoden Retrospektiv wurden Vergiftungsfälle bei Kindern und
Jugendlichen aus 6 deutschen Giftnotrufzentralen (2012–2016 und 2002–2016)
ausgewertet. Kategorielle Daten sind als Mittelwerte±Standardabweichung,
häufigste Noxen nach Punkten angegeben.
Ergebnisse Die Anruferzahl insbesondere der Laien nahm ab 2002
deutlich zu. Zwei Drittel der Fälle traten bei Klein- und Vorschulkindern
auf, häufiger bei Jungen (50%) als bei Mädchen (44%), bei Jugendlichen
überwiegen weibliche Patienten (>60%). Im Alter<14 Jahre sind
Intoxikationen auf Unfälle in Haushalt, Kindertagespflege oder Schulen
zurückzuführen (>95%), bei Jugendlichen treten suizidale Intoxikationen
und Abusus (13%) in den Vordergrund. 90% der Fälle verlaufen asymptomatisch
oder leicht, wobei der Anteil der klinisch symptomatischen Fälle mit dem
Alter zunimmt (Jugendliche 13% vs. Säuglinge 1%). Vergiftungen mit
Medikamenten stellen bei Jugendlichen die häufigste Gruppe dar, bei Kindern
tensidhaltige Reinigungsmittel und Kosmetika, Sanitärreinigungsmittel,
Tabak, Knicklicht und Entkalker in Lösung.
Diskussion und Schlussfolgerung Stetig steigende Anruferzahlen von
Fachpersonal und Laien veranschaulichen die Bedeutung der
Giftnotrufzentralen. Obwohl Vergiftungen bei Kindern und Jugendlichen meist
asymptomatisch oder mit leichten Symptomen verlaufen, darf die Relevanz
präventiver Maßnahmen v. a. bei Kindern<7 Jahren nicht unterschätzt
werden.
Cost-effectiveness of Carbetocin versus Oxytocin for Prevention of Postpartum Hemorrhage Resulting from Uterine Atony in Women at high-risk for bleeding in Colombia
Authors: Gil-Rojas Yaneth , Lasalvia Pieralessandro , Hernández Fabián , Castañeda-Cardona Camilo , Rosselli Diego 
Published: June 2018

Objective To assess the cost-effectiveness of carbetocin versus oxytocin for prevention of postpartum hemorrhage (PPH) due to uterine atony after vaginal delivery/cesarean section in women with risk factors for bleeding.
Methods A decision tree was developed for vaginal delivery and another one for cesarean, in which a sequential analysis of the results was obtained with the use of carbetocin and oxytocin for prevention of PPH and related consequences. A third-party payer perspective was used; only direct medical costs were considered. Incremental costs and effectiveness in terms of quality-adjusted life years (QALYs) were evaluated for a one-year time horizon. The costs were expressed in 2016 Colombian pesos (1 USD = 3,051 Col$).
Results In the vaginal delivery model, the average cost of care for a patient receiving prophylaxis with uterotonic agents was Col$ 347,750 with carbetocin and Col$ 262,491 with oxytocin, while the QALYs were 0.9980 and 0.9979, respectively. The incremental cost-effectiveness ratio is above the cost-effectiveness threshold adopted by Colombia. In the model developed for cesarean section, the average cost of a patient receiving prophylaxis with uterotonics was Col$ 461,750 with carbetocin, and Col$ 481,866 with oxytocin, and the QALYs were 0.9959 and 0.9926, respectively. Carbetocin has lower cost and is more effective, with a saving of Col$ 94,887 per avoided hemorrhagic event.
Conclusion In case of elective cesarean delivery, carbetocin is a dominant alternative in the prevention of PPH compared with oxytocin; however, it presents higher costs than oxytocin, with similar effectiveness, in cases of vaginal delivery.
L1-referenced phonological processing in Japanese-English bilinguals
Authors: Broekhuyse Michelle , Taft Marcus 
Published: June 2018

Abstract
Previous research investigating second-language processing difficulties experienced by Japanese native speakers is consistent with the notion that these individuals are representing foreign words in terms of their native phonological structure at an abstract underlying level. This issue is illustrated here by an experiment that compared Japanese-English bilinguals and native English speakers on their immediate recall of English pseudo-words. Recall performance was dependent on the number of phonemes within list items for the monolingual participants, but on the number of morae for the Japanese bilinguals, indicating that they were indeed automatically activating “Japanized” abstract representations upon encountering the non-native constructions. It is particularly noteworthy that this appeared to be true regardless of the age at which the bilinguals had learnt English.
Cognitive-linguistic skills and reading and writing in Korean Hangul, Chinese Hanja, and English among Korean children
Authors: Cho Jeung-Ryeul 
Published: June 2018

Abstract
This study examined the contribution of orthographic awareness (OA), rapid automatized naming of numbers (RAN), phonological awareness (PA), morphological awareness (MA) in L1 Korean to word reading and writing in L1 Korean Hangul, Chinese Hanja, and L2 English among 98 Korean 5th graders. Korean language and orthography have relatively transparent phonological and morphological structures. Korean children learn Hangul in kindergarten, Hanja in elementary school as an elective subject, and English in Grade 3 as an L2. Results showed that Korean OA accounted for significant variance of Hangul reading; and Korean PA explained English reading and writing and Hangul writing; Korean MA explained writing in Hangul, Hanja, and English; RAN explained English reading only. These results suggest that MA is the meta-linguistic skill that transfers across alphabetic and non-alphabetic languages, whereas PA transfers across alphabetic languages. However, orthographic awareness is language-specific.
Constituent processing or Gestalt processing?
Authors: Pae Hye K. , Kim Sun-A , Luo Xiao (Peter) 
Published: June 2018

Abstract
This study examined how native speakers of Korean extracted letter-feature information from mutilated texts (i.e., top-half and bottom-half), compared to native speakers of Chinese and English. Hypothesized were (1) the upper-part saliency and (2) L1 script effects on L2 reading. A computer-based naming test was administered. Results showed eminent upper-part effects possibly due to more ascenders being at the top than descenders at the bottom of the English letters, but the magnitude of the effects was different among the three groups. Overall, the Korean group seemed to rely more on letter-constituent information drawn from letter features, while the Chinese participants are likely to rely on gestalt information of the word. The results were interpreted with L1 script effects and typology relatedness.
Ḥassāniyya Arabic in contact with Berber
Authors: Taine-Cheikh Catherine 
Published: June 2018

Abstract
Generally speaking, it is at the edges of the Arabic speaking world that one finds the most borrowings and where the influence
of contact on the internal development of Arabic is most visible. Although Mauritanian Ḥassāniyya is an exception to this
general trend (Taine-Cheikh 1994, 2007),
the dialect has nonetheless retained traces of the region’s past and namely of the very gradual disappearance of Zenaga
Berber.
My goal here is to assess, based on the study of a particular lexical sub-category (verb forms with quadriliteral roots), the
influence Berber may have had on lexical formation in Ḥassāniyya Arabic.