bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptome profiling of laser-captured germ cells and functional characterization of zbtb40 during MT-induced spermatogenesis in orange-spotted grouper (Epinephelus coioides)
Authors: Liu Xiaochun , Wu Xi , Yang Yang , Zhong Chaoyue , Guo Yin , Li Shuisheng , Lin Haoran 
Published: July 2019

Abstract
Background: Spermatogenesis is an intricate process regulated by a finely organized network. The orange-spotted grouper (Epinephelus coioides) is a protogynous hermaphroditic fish, but the process of its spermatogenesis is not well-understood. In the present study, transcriptome sequencing of the male germ cells from orange-spotted grouper was performed to explore the molecular mechanisms underlying spermatogenesis.

Results: In this study, the orange-spotted grouper was induced to change sex from female to male by 17alpha-methyltestosterone implantation. During the artificial spermatogenesis, different cell types from cysts containing spermatogonia, spermatocytes, spermatids, and spermatozoa were isolated by laser capture microdissection. Subsequently, transcriptomic analysis for the isolated cells were performed. A series of genes was used to verify and investigate the expression patterns in spermatogenesis. Furthermore, we also analyzed the expression of the same set of genes involved with steroid metabolism and sex throughout spermatogenesis (early-mid, late, and maturing stages) in the orange-spotted grouper. Several generally female-related genes took significantly changes in sex reversal hinted that the female-related genes in previously recognized may also play vital roles in spermatogenesis and sex reversal. In the transcriptomic data, we focused on zbtb family genes, which may be related to the process of spermatogenesis. Their expression patterns and cellular localization were examined, and the location of Eczbtb40 in different gonadal stages was investigated. We found that Eczbtb40 was expressed throughout spermatogenesis. These preliminary findings suggest that Eczbtb40 is highly conserved during vertebrate evolution and plays roles in spermatogenesis. Besides, the expression of Eczbtb40 and Eccyp17a1a overlapped in male germ cells, especially spermatogonium and spermatocyte, which suggested that Eczbtb40 might interact with Eccyp17a1a participant in spermatogenesis and sex reversal.

Conclusions: The present study first depicted RNA sequencing of the male germ cells from orange-spotted grouper, and identified many important functional genes and pathways involved in spermatogenesis. The Eczbtb40 gene was subjected to molecular characterization and expression pattern analysis. These results will contribute to future studies of the molecular mechanism of spermatogenesis and sex reversal.
Drug Therapy Problem and its Contributing Factors among Pediatric patients with Infectious Diseases admitted to Jimma University Medical Center, South West Ethiopia: Prospective observational study
Authors: Feyissa Desalegn Mechessa , Melaku Tsegaye , Dessalegn Dula 
Published: July 2019

Abstract
Background: Drug therapy problem is a significant challenge to provide high quality health care service for the patients. It is associated with morbidity, mortality, increased hospital stay and reduced quality of life. Moreover, pediatric patients are quite susceptible to drug therapy problems.Thus this study aimed to assess drug therapy problem and its contributing factors among pediatric patients diagnosed with infectious disease admitted to pediatric ward of Jimma university medical center, from April ,2018 to June 30, 2018.
Methodology: Prospective observational study was conducted among pediatric patients with infectious disease admitted to Jimma university medical center from April 01,2018 to June 30, 2018. Drug therapy problems were identified by using Cipolle’s and strand’s drug therapy problem classification method. Patient’s written informed consent was obtained after explaining the purpose of the study. Patient’s specific data were collected using structured questionnaire. Data was entered into Epi data version 4.0.2 and then exported to statistical software package version 21.0 for analysis. To identify predictors of drug therapy problems occurrence, multiple stepwise backward logistic regression analysis were done. The 95% CI was used to show the accuracy of data analysis and statistical significance was considered at p-value <0.05.
Results: A total of 304 pediatric patients were included in the study. Of these, 226(74.3%) patients had at least one drug therapy problem during their hospital stay. A total of 356 drug therapy problems were identified among two hundred twenty six patients. Non-compliance (28.65%) and dose too low (27.53%) were the most common type of drug therapy problems while disease comorbidity [AOR=3.39, 95% CI= (1.89-6.08)], Polypharmacy [AOR=3.16, 95% CI= (1.61-6.20)] and more than six days stay in hospital [AOR=3.37, 95% CI= (1.71-6.64) were independent predictors of drug therapy problem occurrence.
Conclusion and recommendation: Drug therapy problems were common in pediatric patients with infectious disease in the study area. Presence of comorbidity, Polypharmacy and Prolonged hospital stay were the predictors of drug therapy problem in this finding. Therefore, to overcome the significant gaps in pediatric pharmaceutical care, clinical pharmacists, Pediatricians and other health care professional have to work in collaboration.
Keywords: Drug therapy problem, Pediatric, infectious disease, Ethiopia
Effect Of Long Term Inhaled Corticosteroid Therapy On Adrenal Suppression, Growth And Bone Health In Children With Asthma
Authors: Anuradha K W D A , Prematilake G L D C , Batuwita B A U I , Kannangoda KASR , Hewagamage U S , Wijeratne S , Lankatilake Kantha , de Silva KSH 
Published: July 2019

Abstract
EFFECT OF LONG TERM INHALED CORTICOSTEROID THERAPY ON ADRENAL SUPPRESSION, GROWTH AND BONE HEALTH IN CHILDREN WITH ASTHMA
Anuradha KWDA1, Prematilake GLDC2, Batuwita BAUI3, Kannangoda KASR4, Hewagamage US5,Wijeratne S6, Lankatilake Kantha7, de Silva KSH8

Abstract
Background
Primary objective was to describe the effect of long term inhaled corticosteroid therapy (ICS) on adrenal suppression, growth and bone health in children with Asthma. Secondary objectives were to describe the association between the dose of ICS and duration of therapy on adrenal functions, growth and bone health in children with asthma, describe their growth ,biochemical parameters associated with bone health and vitamin D levels in comparison to an age and sex matched group of children with asthma who were not on long term ICS.
Method
Seventy children with asthma on ICS and 70 controls were studied. The estimated adult heights were interpreted relative to their Mid Parental Height (MPH) range. Serum calcium, alkaline phosphatase and vitamin D levels were assayed in both groups and cortisol value at 30 minutes following a low dose short synacthen test was obtained from the study group. The average daily dose of ICS was categorized as low, medium and high (100-200, 200-400, >400 ug /day ) respectively according to published literature.
Results
Heights of all children were within the MPH range. There was no statistically significant difference in the bone profiles and vitamin D levels between the two groups (Ca: p=0.554, vitamin D: p=0.187) but vitamin D levels were insufficient (<50 nmol/l) in 34% of cases and 41% of controls. Suppressed cortisol levels were seen in 24%. Doses of ICS were low, medium and high in 56%, 32% and 12% of children respectively. The association between adrenal suppression with longer duration of therapy (p<0.01) and with increasing dose of ICS (p<0.001) were statistically significant.
Conclusion
ICS had no impact on the growth and bone profiles but its dose and duration were significantly associated with adrenal suppression.
Key Words- Asthma, Inhaled corticosteroids, Growth, Adrenal suppression, Bone Metabolism, Vitamin D
Neuroprotective Effects of Idebenone on hydrogen peroxide (H2O2)-induced Oxidative Damage in Retinal ganglion cell-5 (RGC-5) Cells
Authors: Wang Yuping , Wang Jing , Zhang Xi , Feng Yifan , Yuan Yuanzhi 
Published: July 2019

Abstract
Purposes
To investigated the neuroprotective effect of Idebenone against H2O2-induced oxidative damage in RGC-5 cells.
Methods
RGC-5 cells were treated with different concentrations (5, 10, 20μM) of idebenone for 12h prior to addition of 300µM H2O2 for 12 h. The apoptosis of RGC-5 cells were detected by flow cytometry. The changes of mitochondrial membrane potential were detected by JC-1 staining. The autophagy in RGC-5 cells was observed by transmission electron microscopy, and the expression level of autophagy-related protein light chain3, Beclin-1 and mitochondrial membrane potential-related protein Cyt-c in RGC-5 cells were measured by Western blot analysis.

Results

Flow cytometry showed that the apoptosis rates in control group, H2O2 group and H2O2-treatment with Idebenone pretreatment groups were (6.48±0.55)%, (27.34±0.51)%, (22.88±0.52)%, (15.45±0.81)%, (12.59±0.58)%, respectively(F = 559.7, P <0.0001). After incubation with H2O2, the number of autophagosomes increased significantly, while which was decreased in H2O2-treatment with Idebenone pretreatment groups. After incubation of RGC-5 cells with H2O2, the mitochondrial membrane potential was significantly decreased, while idebenone could prevent the decrease of MMP. Contrast with control group, LC3 II /I, the expression levels of Beclin-1 and Cyt-c in H2O2 group increased significantly(P<0.05); while contrast with H2O2 group, LC3 II/I, the expression of Beclin-1 and Cyt-c in H2O2-treatment with Idebenone pretreatment groups was significantly decreased(P<0.05).

Conclusion
Idebenone may have protective effects on RGC-5 cells suffering from oxidative damage induced by H2O2 through improving antioxidant capacity, reducing mitochondrial membrane potential decline and the activity of autophagy.
The effect of dot size in random-dot stereograms on the results of stereoacuity measurements
Authors: Zhao Lingzhi , Wu Huang 
Published: July 2019

Abstract
Background

The aim of this study was to evaluate the effect of the size of the dots in random-dot stereograms on the results of stereoacuity measurements.

Methods

A stereopsis measurement system was created using a phoropter and two 4K smartphones. Three dot sizes, including 1×1 pixel, 6×6 pixels, and 10×10 pixels (equivalent to decimal acuity 6.0, 1.0, and 0.6, respectively), were used to form random-dot arrays, and each test pattern had one digit hidden within it. The resulting stereograms were tested on 30 subjects with normal acuity and stereoacuity.

Results

Stereoacuity measured with the 1-pixel dots was significantly worse than that measured with the 6-pixel dots (Mann-Whitney U test, Z=-6.531, P<0.001) and the 10-pixel dots (Z=-6.494, P<0.001). No significant difference was found between 6-pixel dot and 10-pixel dot stereograms (Z=-0.443, P=0.658).

Conclusion

The size of the dots in random-dot stereograms affects the test results significantly when the dots are too small for the eye to resolve.
Quantitative Comparison of Two-dimensional and Three-dimensional Strain Measurement Using MRI Feature Tracking in Repair Fontan Patients and Normal Child Volunteers
Authors: Hu Liwei , Wang Qian , Gregory Barton P , Ouyang RongZhen , Sun Aimin , Guo Chen , Han Tongtong , Zhong Yumin 
Published: July 2019

Abstract
Purpose
The accuracy of 2D and 3D strain analyses was evaluated by comparing strain and cardiac function parameters in Fontan repair patients and normal child volunteers.
Methods
We retrospectively enrolled 32 patients with Fontan circulation and 32 child volunteers who had undergone clinical cardiac magnetic resonance (CMR) assessment of the dominant ventricle with a 1.5-Tesla MRI scanner. Global and regional strain (2D and 3D) of the dominant ventricle in both groups was assessed using CMR feature-tracking. Correlations between cardiac function and strain data were assessed using Pearson’s correlation coefficient values. The intraclass correlation coefficient (ICC) was determined to evaluate repeatability and agreement.
Results: The 3D global radial strain (GRS), global circumferential strain (GCS), and global longitudinal strain (GLS) showed significant differences between the Fontan repair patients and volunteers (3D GRS: 36.35 ± 16.72 vs. 44.96 ± 9.98; 3D GLS: -8.86 ± 6.84 vs. -13.67 ± 2.44; 3D GCS: -13.70 ± 7.84 vs. -18.01 ± 1.78; p < 0.05, respectively). The ejection fraction (EF) and 3D GCS were significantly associated (r = -0.491, p = 0.004). The 3D GCS showed correlations with the indexed end-diastolic volume (EDV) (r = 0.523, p = 0.002) and indexed end-systolic volume (ESV) (r = 0.602, p < 0.001). 3D strain showed good reproducibility, with GCS showing the best inter-observer agreement (ICC, 0.87), followed by GLS (ICC, 0.84) and GRS (ICC, 0.80).
Conclusion:3D GCS is feasible, highly reproducible, and strongly correlated with conventional cardiac function measures. 3D GCS assessments may be useful for monitoring abnormal myocardial motion in patients with Fontan circulation.
Keywords Strain; Feature tracking; Fontan operation; Pediatric
The shape of the glucose response curve during an OGTT heralds β−cell function in a large Chinese population
Authors: Cheng Xinqi , Yang Na , Li Yuxiu , Sun Qi , Qiu Ling , Xu Lingling , Ping Fan , Li Wei , Zhang Huabing 
Published: July 2019

Abstract
Background

The shape of the glucose response during an oral glucose tolerance test can detect β-cell function and insulin resistance. But there were few studies in Chinese, so we aimed to verify the utility of these connections in a large Chinese population.

Methods

A total of 11,866 times of 3-h OGTT were categorized to either a monophasic or a multiphasic group based on the shape of the glucose response. Homeostasis model assessments of fasting insulin resistance, Matsuda index, insulinogenic index and the disposition index were assessed by plasma glucose and serum insulin concentration obtained at fasting or during an OGTT.

Results

Individuals with a monophasic shape had significantly higher glucose, insulin, and had significantly lower insulin sensitivity and impaired β-cell function than multiphasic group. In addition, Individuals were younger with a multiphasic shape compared to those with a monophasic shape.

Conclusion

The monophasic OGTT glucose response curve could reflect impaired β-cell function in a large Chinese population.
Failure patterns after curative resection for intrahepatic cholangiocarcinoma: Possible implications for postoperative radiotherapy
Authors: Yu Wei , Hu Chunxiu , Shui Yongjie , Wu Kui , Zhang Lili , Chen Ying , Li Chao , Xu Jing , Wei Qichun 
Published: July 2019

Abstract
Objectives: To explore the patterns of failures and areas at highest risk of recurrence for postoperative intrahepatic cholangiocarcinoma (IHCC), with the aim to guide IHCC adjuvant radiotherapy.
Methods: Patients with IHCC who had undergone radical surgery at our institution from July 2010 to August 2017 were retrospectively analyzed. The survival and prognostic factors were analyzed by univariate and multivariate analysis. All sites of recurrence were found out and classified as the surgical margin, regional lymph nodes, liver remnant and distant metastasis. According to the recurring area at highest risk, the target volume of adjuvant radiotherapy was proposed.
Results: The median follow-up time was 23.5 months (2-85 months). The median progression free survival (PFS) and overall survival (OS) were 12.1 months and 24.8 months, respectively. Seventy-three (73/127, 57.5%) IHCC patients developed tumor recurrence. Initial recurrences occurred in the potential postoperative radiotherapy (PORT) volume, remnant liver and distant sits were 46 (46/73, 63.0%), 36 (36/73, 49.3%) and 22 (22/73, 30.1%) cases, respectively. Of the 46 patients whose initial recurrence inside the potential PORT volume, 29 (29/73, 39.7%) developed recurrence only inside the potential PORT volume, including 13 tumor bed recurrences, 7 lymph node metastases, and 9 with both tumor bed recurrences and lymph node metastases. The most common lymph node metastases sites were nodes around the abdominal aorta, followed by lymph nodes along the celiac artery, the common hepatic artery, and in the hepatoduodenal ligament.
Conclusions: High proportion of the recurrences occurred only inside the potential PORT volume, implying the necessary of adjuvant radiotherapy with the aim to improve the local-regional control. Surgical margins and lymph node stations No.16a2, 9, 8, 12, 13, and 14 are suggested to be included in the radiation volume.
A cerebral Arteriovenous Malformation mistakenly diagnosed as Dry Eye and Glaucoma: a case report
Authors: Shen Shuhao , Liu Xiaoyong , Chen Jian , Yang Chengyou , Shi Changzheng , Zhou Qing 
Published: July 2019

Abstract
Background

To report a case of a cerebral Arteriovenous Malformation(AVM) with eye symptoms firstly and review the characteristic of this case and the main confusing point for diagnosis of such case.

Case presentation

A 58-year-old women presented to the ophthalmology clinic with 1 and an half year of right eye redness, Ocular hypertension and recurrent headache. One and a half year ago she was diagnosed as right eye dry eye and glaucoma and had received treatment according to diagnosis, however all the treatment did not lead to any improvement of redness and headache. On physical examination, it revealed dry eye and severe corkscrew hyperemia with dilated vessels in the right eye. After we consider that the symptom may be related to intracranial abnormal vessels, Computed tomography angiography and venography (CTA+CTV) were performed and the results showed an arteriovenous malformation (AVM) of right parietal-occipital area in the brain. The AVM was definitely located by the further examination of Digital subtraction angiography (DSA). After the AVM endovascular embolism treatment, the conjunctival congestion of the right eye was significantly relieved and the intraocular pressure was decreased to normal.

Conclusion

In clinical practice, when found corkscrew hyperemia accompanied by neurological symptoms, it might be considered as a result from cerebral vessels diseases, so in this case ophthalmologists diagnosis should combine disease history and imaging examination.
In Silico ADME and QSAR Studies on a Set of Coumarin Derivatives As Acetylcholinesterase Inhibitors Against Alzheimer’s Disease: CoMFA, CoMSIA, Topomer CoMFA, and HQSAR
Authors: More Uttam Ashok , Patel Sameera , Rahevar Vidhi , Noolvi Malleshappa Ningappa , Aminabhavi Tejraj M. , Joshi Shrinivas D. 
Published: July 2019

Background:Alzheimer’s disease (AD) is increasingly being recognized as one of the lethal diseases in older people. Acetylcholinesterase (AChE) has proven to be the most promising target in AD, used for designing drugs against AD.Methods:In silico studies, 2D- or 3D-QSAR like hologram QSAR (HQSAR), Topomer comparative molecular field analysis (Topomer CoMFA), comparative molecular field analysis (CoMFA), and comparative molecular similarity indices analysis (CoMSIA) methods were used to generate QSAR models for acetylcholinesterase inhibitors.Results:Acetylcholinesterase inhibitors used for the present study contain a series of 7- hydroxycoumarin derivatives connected by piperidine, piperazine, tacrine, triazole, or benzyl fragments through alkyl or amide spacer training set compounds were used to generate best model with a HQSAR q2 value of 0.916 and r2 value of 0.940; a Topomer CoMFA q2 value of 0.907 and r2 value of 0.959, CoMFA q2 value of 0.880 and r2 value of 0.960; and a CoMSIA q2 value of 0.865 and r2 value of 0.941. In addition, contour plots obtained from QSAR models suggested the significant regions that influenced the AChE inhibitory activity.Conclusion:In light of these results, this study provides knowledge about the structural requirements for the development of more active acetylcholinesterase inhibitors. In addition, the predicted ADME profile helps us to find CNS active molecules, the obtained prediction compared with well-known AChE inhibitors viz., ensaculin, tacrine, galantamine, rivastigmine, and donepezil. Based on the knowledge obtained from these studies, the hybridization approach is one of the best ways to find lead compounds and these findings can be useful in the treatment of Alzheimer's disease.
Tools for the assessment of breast cancer screening beliefs in women: a literature review
Authors: Liu Na , Wang Jie , Chen Dan-Dan , Sun Wei-Jia , Zhang Wei 
Published: July 2019

Aim
: This review aims to compare the development, characteristics and validity of three widely used breast cancer screening belief tools (the Champion’s Health Belief Model Scale [CHBMS], the Breast Module of a Cancer Awareness Measures [BCAM] and the Breast Cancer Screening Beliefs Questionnaire [BCSBQ]).
Materials & methods:
Literature reports were retrieved from electronic databases, including PubMed, EMBASE, Web of Science and the Cochrane Library, and other references. All three tools had good reliability and validity. Certain significant differences between these tools should be noted.
Results:
CHBMS, BCAM and BCSBQ are valid and reliable instruments within certain populations. Due to the high heterogeneity among the targeted population, their characteristics and those of the instruments should be fully considered for clinical decision-making.
Conclusion:
The conclusion of this review contributes to the development of a more comprehensive and objective instruments based on the deficiencies of the existing studies.
PEMANFAATAN CARBON CURING AMPAS TEBU SEBAGAI BAHAN TAMBAHAN DALAM CAMPURAN BATA BETON (PAVING BLOCK) DITINJAU DARI DAYA SERAP AIR DAN KUAT TEKAN
Authors: Telaumbanua Nofriadi 
Published: July 2019

Paving block merupakan alternatif konstruksi bangunan yang penting sebagai penutup permukaan tanah. Dalam studi ini ampas karbon digunakan sebagai aditif dalam campuran paving blok. Ampas tebu tersebut disembuhkan pada 2000C selama 1,5 jam. Pengawetan karbon disaring menggunakan mesh no.100 atau 150 μm. Paving blok dibuat dengan perbandingan 1: 2: 1. Konsentrasi pengawetan karbon adalah bervariasi sebesar 0%, 2,5%, 5%, 7,5%, 10% dari volume semen. Hasil penyerapan air paving blok konvensional (0%) adalah 2,81%, semakin besar paving block bila menambahkan curing karbon. Hasil uji kuat tekan menunjukkan adanya peningkatan kuat tekan. Nilai pers yang diperoleh dari penelitian ini pada konsentrasi 5%, 7,5%, dan 10% carbon curing menunjukkan paving block untuk memenuhi standar mutu D SNI-03-0691-1996. Hasil penyerapan air sesuai dengan standar SNI-03-0691-1996 dan kekuatan tekan yang meningkat akibat kerapatan partikel material karena pencampuran curing karbon yang mampu mengisi rongga pada material.Kata kunci : Paving Block, Carbon Curing , Penyerapan air, kuat tekan
PENGARUH PENGGUNAAN LKS BERBASIS GUIDED INQUIRY TERHADAP HASIL BELAJAR FISIKA SISWA KELAS X MIPA SMA
Authors: Kurniawan Rudi 
Published: July 2019

Penelitian ini dilatar belakangi oleh kurang terlibatkannya siswa dalam menemukan fakta, konsep, dan prinsip fisika dalam LKS konvensional, yang membuat siswa mendapatkan hasil belajar yang rendah. Peneliti berinovasi menggunakan LKS berdasarkan tuntunan membimbing yang memecahkan masalah yang diberikan oleh guru. Jenis penelitian ini adalah percobaan kuasi dengan desain kelompok kontrol posttest saja. Pengambilan sampel dilakukan dengan teknik cluster random sampling dan uji hipotesis menggunakan uji t. Kelas eksperimen diperoleh nilai rata-rata tes akhir yaitu 80,57 dan persentase aktivitas siswa tersebut 72,38% dan kelas kontrol adalah 72,32 dan 59,11%. Penelitian ini menunjukkan hasil belajar fisika siswa yang bagus dengan menggunakan LKS pada materi kerja dan energi di kelas X MIPA SMA Negeri 1 Pasaman. Kata kunci : LKS, Inkuiri terbimbing, Hasil Belajar siswa