bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome-Wide Discovery of DEAD-Box RNA Helicase Targets Reveals RNA Structural Remodeling in Transcription Termination
Authors: Lai Yu-Hsuan , Choudhary Krishna , Cloutier Sara C , Xing Zheng , Aviran Sharon , Tran Elizabeth J 
Published: March 2019

AbstractRNA helicases are a class of enzymes that unwind RNA duplexes in vitro but whose cellular functions are largely enigmatic. Here, we provide evidence that the DEAD-box protein Dbp2 remodels RNA-protein complex (RNP) structure to facilitate efficient termination of transcription in Saccharomyces cerevisiae via the Nrd1-Nab3-Sen1 (NNS) complex. First, we find that loss of DBP2 results in RNA polymerase II accumulation at the 3′ ends of small nucleolar RNAs and a subset of mRNAs. In addition, Dbp2 associates with RNA sequence motifs and regions bound by Nrd1 and can promote its recruitment to NNS-targeted regions. Using Structure-seq, we find altered RNA/RNP structures in dbp2∆ cells that correlate with inefficient termination. We also show a positive correlation between the stability of structures in the 3′ ends and a requirement for Dbp2 in termination. Taken together, these studies provide a role for RNA remodeling by Dbp2 and further suggests a mechanism whereby RNA structure is exploited for gene regulation.
Parental Perceptions, Beliefs and Attitudes towards Routine Childhood Vaccinations – United Arab Emirates Experience
Authors: H. Rabei, , H. Elamin, , D. AlSultani, , R. Albarghuthi, , H. AlTatari, 
Published: March 2019

Background: Immunizations have been considered by many as one of the greatest inventions of the 20th century. Despite their success, there had been rising dispute in regards to vaccinations, which led to the emergence of skeptics all around the world who are questioning their efficacy and raising concerns about their safety.
While increasing data are published internationally about this topic, little do we know about the figures in the United Arab Emirates (UAE)
Objectives: This study aims to explore the attitudes, beliefs and perceptions of parents across different Emirates in UAE about the routine vaccinations.
The results of the study will help health care providers have better understanding of the compliance to vaccinations in the area and therefore initiate targeted awareness campaigns to address main issues behind vaccine refusal and misconceptions around it.
Methods: An observational, cross-sectional study was approved by the institutional review board in accordance with the regulations of the Health Authority in Abu Dhabi (HAAD). Survey questionnaires consisting of 26 questions of qualitative and quantitative aspects were distributed to randomly selected parents across five different cities in United Arab Emirates.
Results: A total of 397 participants were included in our study. Parents have showed good perception of the effectiveness of vaccines as 90% agreed that vaccines are effective in preventing certain diseases. However, in regards to parents’ knowledge, 44% did not know that some vaccines prevent certain types of cancer. When looking at the rate of vaccination refusal in our study, we found that 10% of our population refuses to vaccinate their children. Three quarters of those did so because of either their belief that their child will develop his/her immunity naturally or that vaccinations are not safe. Also of note, one thirds of parents believed that vaccinations can cause serious side effects while 19% reported that they have not received sufficient information regarding vaccination from their child’s health care provider.
Conclusion: In our study, parents have demonstrated adequate knowledge and attitudes towards vaccinations. However, there still remains parents who refuse to vaccinate their children. Reasons behind the refusal can be tackled by the primary health care providers through adequate counseling and community based campaigns which aims to educate families about the safety and importance of vaccination.
Analysis of gastrointestinal drugs in patients admitted in CCU and ICCU of a tertiary care hospital in Goa, India
Authors: Kerkar Shantadurga S. , Bhandare Padma N. 
Published: March 2019

Background: Gastrointestinal (GI) diseases are a common problem worldwide. They are frequently encountered in non-critical as well as in critically ill hospitalized patients.Methods: This was a prospective and observational study conducted on patients admitted in CCU and ICCU of Goa Medical College and Hospital. Data was collected from the patients’ case records and was analysed referring to WHO prescribing indicators. Data was represented as Mean±SD, number and frequency.Results: A total of 2250 drugs were prescribed, out of which 376 (16.71%) were gastrointestinal drugs. Most common route of administration was oral 323 (85.90%). Prescription patterns of GI drugs were: 275 (73.14%) from National List of Essential Medicines, 20 (5.31%) as fixed dose combinations and 4 (1.06%) by generic names. Pantoprazole 183 (48.67%) was the most frequently prescribed drug for peptic ulcer in present study.Conclusions: From the findings of this study we noted that among the GI drugs used, those for peptic ulcer were the most commonly prescribed. Also majority were from the essential drug list. But prescribing by generic names was low. Review of drug utilization trends is a necessary aid to formulate and modify existing protocols and guidelines to improve treatment outcomes in a given setting.
Comparison of health supplement on strength, stamina and growth of rats
Authors: Pathak Swanand S. , Yadav Nikhil S. 
Published: March 2019

Background: Children require more nutrition as organs grow fast in this age group. Due to abundance of various commercial health supplements in market, parents are often confused about selection of health supplements. Traditional foods are rich source of various nutrients required by growing children. We conducted a study to compare strength, stamina and growth enhancing ability of commercial health supplement and natural health supplement on wistar rats.Methods: Total of 24 wistar rats were included. Animals were divided in four groups. Each group of rats except control group were receiving health supplements. Group I, group II, group III and group IV were receiving health supplement I, health supplement II, natural health supplement and no health supplement (control group) for 60 days. Strength and stamina of wistar rats in each group were analysed using grip strength meter and swim stress test, growth of rats in each group were analysed by measuring body length, tail length and body weight. Group of rat showing maximum growth (body length, tail length and body weight), strength and stamina were analysed.Results: Group II rats showed greater strength and stamina followed by group I, group III and group IV respectively. Group I rats showed maximum growth followed by group II, group III and group IV respectively.Conclusions: In the current study we found that strength and stamina were greater in group II rats followed by group I, group III and group IV respectively also group I rats showed maximum growth followed by group II, group III and group IV respectively.
Analysis of caesarean section rate according to modified Robson’s classification at tertiary care centre in Uttarakhand, India
Authors: Dogra Kusum , Arora Neetu , Sharma Bhawna , Tanwar Meenakshi 
Published: March 2019

Background: High caesarean section rate worldwide including India is matter of concern. The aim of this study is to analyse caesarean section rate at tertiary care centre according to Modified Robson’s classification.Methods: This retrospective study was conducted at Shri Guru Ram Rai Institute of Medical and Health Sciences (SGRRIMHS) and Shri Mahant Indiresh Hospital at Dehradun from April 2018 to September 2018. All women delivered during this period were classified according to modified Robson’s classification using their maternal characteristics and obstetric history. For each group, authors calculated the caesarean section rate within the group and its contribution to overall caesarean section rate.Results: Out of total 1302 women delivered, 395 underwent CS (30.3%).The major contribution to overall caesarean section rate was 33.4% by group 5 (Previous CS, singleton, cephalic, >37weeks) followed by 16.7% by group 1 (nullipara, singleton, cephalic, >37 weeks, spontaneous labour), 12.4% by group 3 ( multipara, singleton, cephalic, >37 weeks, spontaneous labour ).CS rates among various group ranges from 100% among women with abnormal lie (group 9) to 77.5% in nulliparous breech (group 6), 73.7% in previous CS (group 5) and least 11.2% in multipara induced or pre labour CS (group 4).Conclusions: Modified Robson classification is simple, systematic, reproducible and can be effectively utilized in analyzing delivering women. Major contribution to overall caesarean section is made by previous CS.
Emergency peripartum hysterectomy at a tertiary care hospital: analysis of epidemiology, clinical profile, indications and outcome
Authors: E. Anitha , S. Ramalakshmi 
Published: March 2019

Background: Peripartum hysterectomy is a life-saving obstetric procedure that is performed at the time of a caesarean section or postpartum following either vaginal delivery or caesarean section. The purpose of the present study was to determine the incidence, risk factors ,indications ,maternal and neonatal morbidity, mortality and complications of emergency peripartum hysterectomy performed at a tertiary teaching hospital in South India and to compare the results with other reports in literature.Methods: During the period of study between January 2015-December 2016, there were 50 cases of emergency peripartum hysterectomy at Tirunelveli medical college hospital, Tirunelveli, Tamil Nadu, South India. Medical, pathology and operation theatre records were analyzed retrospectively. Details of maternal age, parity, booking status, underlying risk factors, past obstetric history, gestational age at delivery ,mode of delivery ,indications for emergency peripartum hysterectomy, type of operation, intraoperative and post-operative complications, blood components transfusion ,maternal and neonatal outcomes were noted and analyzed.Results: During the 2-year study period there were 50 emergency peripartum hysterectomy out of 14,363 deliveries, a rate of 1 per 294 deliveries (3.4/1000 deliveries). 80% of hysterectomies were performed after caesarean delivery and 20% after vaginal delivery. The two major indications were abnormal placentation (40%) and uterine atony (28%). There were 6 maternal deaths among patients who underwent emergency peripartum hysterectomy during the period of study.Conclusions: Improved antenatal care, correction of anaemia, identification of risk factors for peripartum hysterectomy, timely referral, expedite management, timely decision, availability and liberal use of blood components and appropriate management of post-operative complications by experienced clinical team are the main stay for saving maternal lives. With increasing rate of caesarean section there is rise in the incidence of abnormal placentation.
Primary laryngeal tuberculosis-changing trends and masquerading presentations: a retrospective study
Authors: Ponni Satish , Venkatesan R. Sithananda Kumar , Saxena Sunil Kumar , Suryanarayanan Gopalakrishnan 
Published: March 2019

<p class="abstract"><strong>Background:</strong> Primary laryngeal tuberculosis is one of the common forms of head and neck tuberculosis. There is a changing trend in the manifestations of this disease and this retrospective study highlights the varying masquerading manifestations and the diagnostic and management dilemmas in managing such patients.</p><p class="abstract"><strong>Methods:</strong> This is a retrospective study of patients with laryngeal tuberculosis at a tertiary care hospital. Patients with primary laryngeal tuberculosis without pulmonary involvement where analyzed for the clinical manifestations, the diagnostic difficulties that where encountered and the management of these patients were analyzed. </p><p class="abstract"><strong>Results:</strong> Forty eight cases with laryngeal tuberculosis where identified. 12 patients with concomitant Pulmonary involvement where excluded from the study. Of the 36 patients with primary laryngeal tuberculosis 22 cases presented with atypical features like manifestation as acute epiglottitis (8 cases), mimicking laryngeal malignancy (10 cases) and polypoid lesions of the vocal cord (4 cases).</p><p class="abstract"><strong>Conclusions:</strong> Despite the rarity of primary laryngeal tuberculosis, a high index of suspicion is always necessary in any laryngeal lesions eluding diagnosis considering the varied manifestations of present day laryngeal tuberculosis.</p>
Saying It Like Indians: The Wooster Group’s Cry Trojans!, Sa(l)vage Ethnography, and the Politics of “Playing Injun”
Authors: Hollis Gavin 
Published: March 2019

In The Wooster Group's Cry Trojans!, the Trojan characters are depicted as Red Indians. The theatre company's play with white, American appropriations of Native American culture is the latest in a long line of controversial engagements with race in the company's history, dating back to Route 1 & 9 and The Emperor Jones. It also forms part of their more recent engagement with classical theatre, in particular Shakespeare, as Cry Trojans! has it origins in a much-reviled collaboration between the Wooster Group and The Royal Shakespeare Company in 2012. By their own admission, the Group chose to "Play Indian" to be "as American as possible," and also because (to quote Kate Valk, who played Cressida), they felt they "'... should say it like Indians,’ because I was thinking of English as a second language." The choice then comments on the Group's own play with authenticity, another constant enagement throughout its thrity-plus years history. By mimicking and appropriating Native American and First Nations cultural production (Smoke Signals and Atanarjuat in particular), the Group performs its own uneasy engagement with authenticity. However, in contrast to their staging of The Emperor Jones, which employed blackface to unpack Eugene O'Neill's "idea of a Negro," Cry Trojans! reduces native culture and history to a white, elite history of appropriation and genocide without positing alternative histories of resistance and/or re-appropriation. It does so most expicitly in the final scene, where a blanket, a property associated with Euro-American germ warfare and Native American genocide, is applied first to Troilus and then to Pandarus, the bequeather of poxy diseases, to suggest the imminent demise of the Trojan-Injuns. The Wooster Group seems to be aware of part of their appropriative formulations, and put on display both the work (their adoption of an Indian manner) and the work of art (the acts of appropriation that lead to their adoption of Indian manner): but for a Company so invested in the ironies of post-modernity, they do not in this production seem so aware of the depth of their own unironic complicities in the erasure of indigenous cultures and cultural forms. The production's employment of Native American/First Nations cultural production undermines their own claims of native cultural loss.
THE RELATIONSHIP BETWEEN SELF-ESTEEM, FAMILY RELATIONSHIPS AND SOCIAL SUPPORT AS THE PROTECTIVE FACTORS AND ADOLESCENT MENTAL HEALTH
Authors: Triana Rike , Keliat Budi Anna , Sulistiowati Ni Made Dian 
Published: March 2019

Purpose : Mental disorders begin to occur at the age of 10-29 years about 10-20 %. Protective factors to prevent mental disorders in adolescents were self-esteem, family relationships and social support. The purpose of this study was to determine the relationship between the protective factors of self-esteem, family relationships, and social support and adolescent mental health.
Methodology: This research employs a correlative design. The subjects were 452 students in 8 grade (aged < 15 years old) that chosen by a purposive sampling technique. Data were collected by five questionnaires: demographic data, Rossenberg Self-Esteem Scale, Family Relation Index, Child and Adolescent Social Support Scale, Mental Health Continuum Short Form.
Main Findings: The results show that the protective factors of self-esteem, family relationships, and social support have a positive and significant influence on adolescent mental health (p < 0,05).
Applications: These findings showed the important role of protective factors (self-esteem, family relationships, social support) to adolescent mental health. Schools, parents and mental health nurses need to develop programs to promote mental health by improving the protective factors of self-esteem, family relationships, and social support.
Novelty/Originality: There are no studies involving comprehensive protective factors include individuals, families and communities on adolescent mental health.
Kolaborasi Peta Indonesia dan Model Pembelajaran Teams Games Tournaments (TGT)
Authors: Sakdiyah Siti Halimatus , Triwahyudianto Triwahyudianto , Harun Harun 
Published: March 2019

Penggunaan media Peta Indonesia dan model pembelajaran TGT yang melibatkan siswa sebagai tutor sebaya mudah diterapkan tanpa ada perbedaan antara siswa yang pandai atau kurang, dimana kolaborasi ini dikemas dalam permainan agar menjadi pembelajaran yang lebih efektif dan menyenangkan, sehingga dapat meningkatkan aktivitas dan hasil belajar. Hasil observasi menunjukkan proses pembelajaran terjadi searah sehingga siswa lebih asyik bergurau daripada belajar. Dari kenyataan ini menyebabkan hasil belajar siswa cenderung rendah. Terbukti hanya 39,28% dari 28 siswa yang mencapai ketuntasan belajar, sedangkan 61,11% nilainya dibawah KKM 75.Tujuan penelitian untuk mendiskripsikan peningkatan aktivitas dan hasil belajar siswa kelas IV SD Islam Al Hikmah. Rancangan penelitian menggunakan penelitian tindakan kelas. Penelitian dilakukan pada siswa kelas IV SD Islam Al Hikmah. Subjek dalam penelitian terdiri dari 28 siswa. Nilai hasil belajar siswa kelas IV pada siklus I menunjukkan siswa yang tuntas 21 siswa dengan presentase 75%, sedangkan pada siklus II menunjukkan siswa yang tuntas 24 siswa dengan presentase 85%. Hasil dari penelitian ini dapat disimpulkan bahwa penggunaan Peta Indonesia dan model pembelajaran TGT dapat meningkatkan aktivitas dan hasil belajar siswa kelas IV SD Islam Al Hikmah Gadang Malang.
Perbandingan Pembelajaran PPKn pada Implementasi KTSP dan Kurikulum 2013 di Sekolah Menengah Pertama (SMP)
Authors: Hermuttaqien Bhakti Prima Findiga , Sata Haula Ria , Wadu Ludovikus Bomans 
Published: March 2019

Penelitian ini bertujuan untuk melihat perbandingan pembelajaran PPKn menggunakan KTSP dan Kurikulum 2013di SMP. Metode dalam penelitian ini yaitu deskriptif kuantitatif. Pemilihan sampel pada penelitian menggunakan teknik purposive (nonprobabilty) sampling. Frekuensi jawaban responden ketegori guru pada implementasi KTSP kategori pembukaan sebesar 54.5%, kategori inti sebesar 63.6%, kategori penutup sebesar 63.6% sedangkan penilaian kurikulum 2013 frekuensi jawaban responden kategori pembukaan sebesar 72.7%, kategori inti sebesar 63.6%, kategori penutup sebesar 81.8%. Disimpulkan dari penelitian menunjukkan terdapat perbandingan pembelajaran PPKn dengan implementasi KTSP dan Kurikulum 2013 di SMP Se Kecamatan Turen. Hal ini dibuktikan dengan nilai thitung 2.930 dan ttabel 2.201 yang artinya nilai thitung lebih besar dari ttabel dengan nilai sig 2 tailled 0,008<0,05.
Pengembangan Media Shapes untuk Kreativitas Anak Usia Dini Kelompok B
Authors: Kolta Ermiani N. , Sholeh Agus , Wijayanti Rina 
Published: March 2019

Penelitian ini bertujuan untuk mengetahui bentuk, hasil, kelayakan, kelebihan, kekurangan dari pengembangan media pembelajaran shapes untuk kreativitas anak usia dini. Penelitian ini menggunakan metode Research & Development (R&D) menurut Borg dan Gall. Kelayakan produk didasarkan pada hasil penilaian ahli media, ahli materi, dan anak didik kelompok B Taman Kanak-Kanak (TK) sebagai subjek uji coba. Instrumen pengumpulan data menggunakan observasi, wawancara, angket, dan dokumentasi. Teknik analisis data berupa data kualitatif dan data kuantitatif. Hasil penelitian menunjukan bahwa media shapes yang telah dikembangkan melalui serangkaian uji coba serta validasi ahli dinyatakan layak. Hal ini didukung oleh penilaian ahli media dengan rata-rata skor 3,77 termasuk dalam kategori layak, penilaian ahli materi dengan rata-rata skor 3,6 termasuk kategori layak, uji coba lapangan utama mendapatkan presentase 98,55% termasuk dalam kategori layak, dan uji coba lapangan operasional mendapatkan presentase 97,90% termasuk dalam kategori layak. Berdasarkan hasil penelitian yang telah dilakukan dapat disimpulkan bahwa media shapes untuk kreativitas anak usia dini kelompok B TK layak digunakan.
Biosurfactant production by Pseudomonas aeruginosa isolated from aquaculture farm soil and its optimisation
Authors: Nadella Ranjit kumar , Vaiyapuri Murugadas , kusunur Ahamed Basha , Joseph Toms Cheriath , Velayudhan Lalitha Kuttanappilly , Mothadaka Mukteswar Prasad 
Published: March 2019

In the present study, aquaculture farm soil was screened for the biosurfactant producing bacteria. Total of 43 distinct morphological colonies were isolated from the farm soil and their biosurfactant production was evaluated by employing different screening methods. Fourteen biosurfactant producing bacterial isolates were selected based on the formation of dark blue halos on CTAB agar, emulsification index, oil spreading assay and BATH assay. Based on the results, bacterial isolate (BHA 9) showed highest production of biosurfactant and selected for further studies. Biochemical characterisation revealed that the bacterial isolate responsible for biosurfactant production is Gram negative, slender long rod shape bacteria and oxidase and catalase positive. Molecular characterisation of 16S r-DNArevealed that it belongs to Pseudomonas aeruginosa . Optimization studies were carried out at different temperatures (25, 30, 35 and 40 o C) using four different carbon sources (1%) i.e ., glucose, sucrose, maltose and starch and four nitrogen sources (1%) viz ., peptone, ammonium nitrate, beef extract and yeast extract at different pH (6, 7, 8, 9 and 10) and NaCl levels (0.50, 1, 1.50 and 2%). Emulsification index and the bacterial biomass (OD 600 ) were recorded at 24, 48, 72 and 96 h intervals. Optimum condition for biosurfactant production by this bacterium was achieved when glucose and yeast extract was used as carbón and nitrogen sources, respectively maintaining a temperature of 35 o C, pH 8 and NaCl 1.5% measured in terms of emulsification index and bacterial biomass. This is the first reported study for the biosurfactant producing bacteria from aquaculture farm soil which may find its application in various fields.