bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reversible Inhibition of the In Vitro Coagulation of Human Plasma by Lectins
Authors: Freyssinet J-M , Thevenon D , Souque A , Suscillon M 
Published: July 2018

SummaryWheat germ agglutinin (WGA) and concanavalin A (Con-A) (also red kidney bean agglutinin, PHA) have been found to be inhibitors of plasma clotting in vitro. At 40 µg/ml and 250 µg/ml (4.4 µM and 10 µM in carbohydrate binding sites, final concentrations) respectively, WGA and Con-A are able to double the activated partial thromboplastin time of normal human control plasma. Their inhibitory effect is due to their capacity to interact with the carbohydrate portion of blood clotting factors. It is totally abolished in the presence of specific saccharides for WGA or Con-A and is attenuated in the presence of 4% (v/v, final concentration) of human erythrocytes. The action of WGA is mediated by its ability to interact with N-acetylneuraminic acid. When purified phospholipid vesicles plus kaolin are used as an activator instead of cephalidin, this effect persists to the same extent. These two lectins also prolong the plasma clotting time using Russell's viper venom plus purified phospholipid vesicles as an activator. Quick's time was also prolonged by WGA and Con-A but to a lesser extent in this case. WGA can interact directly with some purified blood clotting factors (IX, X and II) in a classical lectin-glycoprotein precipitin reaction. When assessed at individual factors level in whole plasma using clotting assays, direct inhibitions by WGA are only apparent.
In Vivo Interactions of Autoantibodies to Factor VIII with the Factor VIII Complex
Authors: Allain J P , Croissant M P , Lerolle D , Houbouyan L , Zuzel M , Frommel D 
Published: July 2018

SummaryTwo non-haemophilic elderly patients who had developed autoantibodies to factor VIII were studied over a period of 9 months to 5 years. Sequential measurements of antibody to factor VIII (anti-VIII: C), factor VIII coagulant activity (VIII: C), factor VIII coagulant antigen (VIII: CAg), factor VIII-related antigen (VIIIR: Ag), and factor VIII ristocetin cofactor (VIII: WF) were performed. Before treatment, low VIII: C, norlnal or increased VIII: CAg and high VIIIR: Ag levels were found and were indicative of the presence of circulating immune complexes. Immunosuppressive therapy induced progressive correction of VIII: C and VIIIR: Ag values. High levels of VIII: CAg subsided in the patient who relapsed. It is suggested that antibodies to factor VIII bind and remove VIII: C from the circulation thereby inducing an increased synthesis of VIII: CAg which may be associated with an augmented release or production of VIIIR: Ag.
The State of Actin Polymerization in Tetracaine-Treated Platelets
Authors: Davies Gregg E , Palek Jiri 
Published: July 2018

SummaryAlthough there is considerable evidence that platelet activation is associated with polymerization of actin, it is not known whether there is some pre-existing F-actin even before activation. We have examined the state of actin polymerization in nonactivated platelets by deoxyribonuclease assay of G-actin and total actin. To fully suppress activation, platelets were prepared in the presence of tetracaine. The G-actin/total actin ratio in tetracaine-treated platelets prepared by three different methods is significantly less than one (average of all results = 0.61). We conclude that about 39% of total actin in nonactivated tetracaine-treated platelets may be present as F-actin.
The Use of Desmopressin Acetate (DDAVP) as a Test of the Fibrinolytic Capacity of Patients – Analysis of Responders and Non-Responders
Authors: Brommer E J P , Barrett-Bergshoeff M M , Allen R A , Schicht I , Bertina R M , Schalekamp M A D H 
Published: July 2018

SummaryIntravenous infusion of desmopressin (DDAVP, 0.4 μg/kg b.w. in 12’) causes an increase in the level of extrinsic plasminogen activator, measured in plasma euglobulin fractions with added C1-inactivator on fibrin plates. A poor response or no response at all was elicited in two out of 21 patients with spontaneous thrombosis, 18/38 with hyperlipoproteinaemia and 10/14 with terminal renal insufficiency requiring haemodialysis.Haemodilution during the first 30’ after starting the DDAVP-infusion occurred both in responders and in non-responders; so did haemodynamic reactions: increase in heart rate, drop in diastolic blood pressure, facial flushing. The rise of fibrinolytic activity was shown not to be associated with decreased hepatic blood flow. Normal factor VIII-rises in “non-responders” indicate the responsiveness of the receptive organs, including the hypothalamus, to DDAVP.Despite a normal baseline level of fibrinolytic activity in the blood, as occurs for instance in terminal renal insufficiency, the vascular endothelium may be refractory to stimulation. In some patients, especially in type IV hyperlipoproteinaemia, a selective defect of the release of plasminogen activator is postulated. In subjects with low fibrinolytic activity at rest, as observed in spontaneous thromboembolism and in hypertriglyceridaemia, the failure to release plasminogen activator upon stimulation with DDAVP might be a consequence of an impairment of synthesis as well.
Inhibition of Human and Rat Platelet Aggregation by Extracts of Mo-er (Auricularia auricula)
Authors: Agarwal K C , Russo F X , Parks R E 
Published: July 2018

SummaryHot water extracts of Mo-er (1 gm by 15 ml of water), an oriental food (Auricularia auricula), inhibit strongly both human and rat platelet ADP-induced aggregation. HPLC analysis of two varieties of Mo-er, A.auricula and A.polytricha (a black tree fungus), shows that they contain adenosine (Ado), 133 and 154 micrograms per gram of dry fungus, respectively. The inhibition of ADP-induced platelet aggregation by Mo-er extracts and by Ado was compared. Mo-er extracts caused a more rapid onset and a longer duration of inhibition than produced by equivalent amounts of Ado. Furthermore, Mo-er extract treated with adenosine deaminase to degrade the Ado retained the capacity to inhibit platelet aggregation. The inhibitory effects of Mo-er extracts on ADP-induced human platelet aggregation are greatly potentiated by the inhibitors of cyclic AMP phosphodiesterase such as oxagrelate (phthalazinol) and papaverine. The inhibition of platelet aggregation is only partially blocked by 2’,5’-dideoxy-adenosine (DDA), an inhibitor of platelet adenylate cyclase and 5’-deoxy, 5’-methylthioadenosine (MTA), an antagonist of Ado receptors. ADP-induced rat platelet aggregation is strongly inhibited by Mo-er extracts, but not by Ado. This inhibition is not reversed by either DDA or MTA. These findings indicate that Mo-er extracts contain an agent (or agents) in addition to Ado, that blocks platelet aggregation by a mechanism that does not involve the platelet cyclic AMP system.
Effects of Ethanol Intoxication and Hangover on Plasma Levels of Thromboxane B2 and 6-Keto-Prostaglandin F1α and on Thromboxane B2 Formation by Platelets in Man
Authors: Kangasaho M , Hillbom M , Kaste M , Vapaatalo H 
Published: July 2018

SummaryWe detected a significant decrease in plasma thromboxane B2 (TXB2) and 6-keto-prostaglandin F1α (6-keto-PGF1α) levels together with a significant increase in plasma 6-keto-PGF1α/TXB2 ratio in young healthy non-alcoholic male volunteers after acute ingestion of ethanol (1.5 g/kg). Paradoxically, during ethanol intoxication and the following hangover a significant increase in ADP-induced formation of TXB2 by platelet rich plasma could be observed, which suggests that ethanol intoxication via some unknown mechanism sensitizes platelets to produce TXB2. Whether these observations contribute to the increased risks of subarachnoid haemorrhage or ischaemic brain infarction among occasional heavy drinkers recently described by us remains to be proved.
Repeated Occurrence of Skin Necrosis Twice Following Coumarin Intake and Subsequently During Decrease of Vitamin K Dependent Coagulation Factors Associated with Cholestasis
Authors: Hofmann V , Frick P G 
Published: July 2018

SummaryA female patient is described who developed skin and subcutaneous fat necrosis on two occasions after intake of acenocoumarol.Several months later identical skin changes occurred during an episode of cholestasis associated with a prolongation of the prothrombin time to an extent comparable with therapeutic anticoagulation; intake of oral anticoagulants could be excluded.This association gives new insights in the pathogenetic mechanisms responsible for the so-called coumarin necrosis and indicates that it may be not due to drug toxicity or allergy.
Inhibition of Activated Coagulation Factor VII by Normal Human Plasma
Authors: Dahl P E , Abildgaard U , Larsen M L , Tjensvoll L 
Published: July 2018

SummaryAn amidolytic assay system with tissue thromboplastin (Tpl), purified coagulation factors VII and X, and the chromogenic substrate S-2222 was developed. Antithrombin III (AT) accounts for about one third of the total inhibition exerted by normal plasma in this test system. This effect of AT was prevented by adding purified AT blocking antibodies. Normal plasma and serum showed approximately similar inhibitory effects. The inhibition was probably directed against activated factor VII (F VIIa). Gel filtration of adsorbed normal plasma on Ultrogel AcA 34 showed three inhibitory peaks which were different from AT.
Home-Diagnosis of Deep Venous Thrombosis with Impedance Plethysmography
Authors: Peters S H A , Jonker J J C , de Boer A C , den Ottolander G J H 
Published: July 2018

SummaryIn order to assess the value of I.P.G. for the diagnosis of D.V.T. in general practice, an I.P.G. was carried out by a skilled technician in 255 consecutive patients with suspected D.V.T. at home. Ascending venography was carried out in 185 of these patients. In addition, blood for assay of AT III, platelet count, fibrinogen, a2-antiplasmin, ethanol gelation test and spontaneous platelet aggregation was collected at the time the I.P.G. was performed. In 61 patients (33%) venography showed the presence of D. V.T., and was negative in the remaining 124 patients. I.P.G. was positive in 51 of the 61 patients with D.V.T., a sensitivity of 84%. I.P.G. was normal in 115 of the 124 patients with a negative venogram, a specificity of 93%. The sensitivity of the I.P.G. for proximal vein thrombosis was 92% and for calf vein thrombosis 68%. Mean a2-antiplasmin concentration was significant (p Ã0.05) lower (101 ± 15%, mean ± SD) inpatients with D.V.T. compared with patients with a normal venogram (107 ± 11%, mean ± SD). No differences between the two groups were observed in the other coagulation parameters assayed, and none was of diagnostic value, either alone or in combination with I.P.G. This study shows that I.P.G. is of potential value for the home diagnosis of D.V.T., in particular proximal vein thrombosis. This is potentially	clinically useful, because these thrombi are thought to carry a high risk for pulmonary embolism.
Effects of Etamsylate on Platelet Functions and Arachidonic Acid Metabolism
Authors: Okuma M , Takayama H , Sugiyama T , Sensaki S , Uchino H 
Published: July 2018

SummaryThe effects of etamsylate on human platelet aggregation and ATP release as well as on the arachidonate metabolism by the platelet have been studied. Etamsylate enhanced these platelet functions induced by arachidonic add (AA), thromboxane A2, collagen and caldum ionophore A23187 but not those induced by ADP and epinephrine. In experiments with cyclooxygenase-inhibited platelets, AA-induced platelet aggregation was completely inhibited and it was not enhanced by etamsylate, while A23187-induced aggregation was partially inhibited and this aggregation was enhanced by etamsylate. Platelet AA metabolism including thrombin-induced AA liberation from phospholipids as well as the lipoxygenase and cyclo-oxygenase pathways was not significantly affected by etamsylate. These results suggested that etamsylate enhanced platelet response to thromboxane A2 and calcium ionophore and that this could be included as a mechanism for its potentiating effect on platelet functions.
Effect of a Selective Thrombin Inhibitor MCI-9038 on Fibrinolysis In Vitro and In Vivo
Authors: Tamao Y , Yamamoto T , Kikumoto R , Hara H , Itoh J , Hirata T , Mineo K , Okamoto S 
Published: July 2018

SummaryThe effect of a selective thrombin inhibitor, (2R, 4R)-4-methyl-1- [N2- [(3-methyl-1,2,3,4-tetrahydro-8-quinolinyl)sulfo-nyl]-L-arginyl]-2-piperidinecarboxylic acid (MCI-9038), on the fibrinolysis induced by t-PA and u-PA was studied in vitro and in vivo. MCI-9038 remarkably reduced the lysis time of the plasma clot generated by the addition of calcium chloride to the plasma at the concentration ranging from 0.01 to 0.3 μM. Heparin also reduced the plasma clot lysis time with a lower effect than MCI-9038. The fibrin crosslinkage in the plasma clot was inhibited by MCI-9038 or heparin. MCI-9038 potently inhibited the factor XIIIa generation from factor XIII by thrombin.The effect on the in vivo thrombolysis was studied on the arterial thrombosis generated by the endothelial cell injury of the rabbit carotid artery by acetic acid. t-PA dissolved the thrombi with the infusion at 0.96 mg/kg over 2 h without a significant activation of a systemic fibrinolysis. u-PA dissolved the thrombi with the infusion at 180,000 and 360,000 IU/kg over 2 h. At a dose of 0.48 mg/kg t-PA or 90,000 IU/kg u-PA, the thrombi were not dissolved, but the combined use of MCI-9038 at 1.2 mg/kg over 2 h effectively dissolved the thrombi. Thus, combination of MCI-9038 with plasminogen activators accelerated thrombolysis of an experimental thrombosis in rabbits.
Correlation of Antibodies to LAV/HTLV III in Hemophiliacs with the Use of Virus-Inactivated Clotting Factors
Authors: Dietz Brigitte , Klose H J , Gürtler L , Eberle J , Deinhardt F , Köhler-Vajta Katalin , Peller P 
Published: July 2018

SummaryThe retrovirus LAV/HTLV III, highly likely to be responsible for the acquired immunodeficiency syndrome (AIDS) in some recipients of blood products, can be inactivated by chemical and/ or heat treatment, so the use of virus-inactivated factor VIII and factor IX preparations for treating hemophilia A and B has become important. We examined hemophilic children and found that those children treated since 1979 with virus-inactivated preparations did not develop antibodies against LAV/HTLV III. In contrast, 77% of patients treated with conventional factor VIII or factor IX preparations had antibodies against this virus.
Role of Heparin in Tumor Cell-Induced Platelet Aggregation
Authors: Yamamoto Kazuo , Kitagawa Hisayo , Tanoue Kenjiro , Tsuruo Takashi , Yamamoto Naomasa , Yamazaki Hiroh 
Published: July 2018

SummaryB16 mouse melanoma cell lines (B16F1, B16F10 and B16BL6) were able to induce platelet aggregation, and concomitant release of ATP in heparinized platelet-rich plasma (PRP). In citrated PRP, these tumor cells did not induce platelet aggregation. Addition of heparin to citrated PRP enabled these tumor cells to induce aggregation. In heparinized PRP, platelet aggregates induced by B16F10 cells were dissociated by the addition of either 4 mM EDTA, 10 mM CaCl2 or 0.1 μg/ml protamine sulfate. B16F10-induced aggregation in heparinized PRP was inhibited by preincubation with anti-fibronectin antibody, but not with antifibrinogen or anti-von Willebrand factor antibodies. B16F10 cells induced aggregation in washed platelet suspension with the addition of heparinized platelet-poor plasma (PPP). Cryoprecipi-tate from human plasma showed the same effect in the presence of heparin if substituted for PPP. The mixture of purified fibronectin, von Willebrand factor, fibrinogen and heparin were less effective than cryoprecipitate on B16F10-induced aggregation of washed platelets. The results suggest that an interaction between fibronectin and heparin may be important in tumor cell-induced aggregation.
Neonatal Intrathoracic Gastric Volvulus in Marfan's Syndrome
Authors: Serradilla Javier , Bueno Alba , Torre Carlos , Gamarra Eduardo , Romo Martha , Nava Hurtado de Saracho Francisco , Barrial María , Cervantes Manuel , Santamaria Manuel 
Published: July 2018

AbstractWe report a 12-day-old male who was admitted with vomiting because of an unusual early complication of Marfan's syndrome (MS): a sliding hiatal hernia. Initial ultrasound showed no stomach at its normal position and the chest X-ray presented an intrathoracic gas bubble with the nasogastric tube inside. An upper gastrointestinal contrast study confirmed the complete herniation of the stomach into the thorax. Via an exploratory laparotomy it was carefully reintroduced into the abdomen, following a hiatal reconstruction. A Thal fundoplication and a gastrostomy were also performed to guarantee its fixation. Although characterized by cardiac/aortic abnormalities, MS should be considered in any infant with hiatal/paraesophageal hernia, which should be repaired early to avoid gastric ischemia/volvulus.
Volumenmanagement bei Sepsis und septischer Schock
Authors: Kochanek Matthias , Böll Boris 
Published: July 2018

AbstractSepsis and septic shock are common diseases with high mortality rates. Although volume therapy has been a central component of sepsis therapy for decades, the choice of optimal fluid and fluid intake is unclear. This paper summarizes findings on pathophysiology, clinical trial results, and current recommendations for optimal volume and fluid management in sepsis.
Comparison of Core Muscles Endurance in Sited Occupied Staff and Non-sited Occupied Staff of Shiraz University of Medical Sciences
Authors: Jabarifard Fatemeh , Ghafarinejad Farahnaz , Pirouzi Soraya , Samani Mahbobeh 
Published: July 2018

Abstract
Objective Core muscle dysfunction might serve as a risk factor for future musculoskeletal dysfunctions considering the high percentages of adults employed in mainly sedentary occupations in Iran, there is a need to clarify the strength of evidence on the potentially deleterious impact of prolonged sitting at work on the biomechanics of core/trunk muscles. This study aims to evaluate trunk/core muscle endurance in employees of seated and non-seated jobs in Shiraz University of Medical Sciences.
Method A total number of 100 employees of Shiraz University of Medical Sciences (SUMS) were studied in 2 groups, seated jobs (n=50) and non-seated jobs (n=50). Seated jobs (office employees) defined as the jobs’ nature requiring the employees to sit more than half of their work time in a day, whereas non-seated jobs were those requiring less than half a workday to sit. Trunk endurance time measured by the 4 different stabilization tests including McGill’s trunk flexor endurance test, Sorenson’s trunk extensor endurance test and right and left trunk lateral flexor endurance test (Side-Bridge test).
Results Statistical analysis was performed using the SPSS software version 21 (SPSS, Chicago, IL) Independent t test was used individuals in non-seated group had a statistically significant higher trunk endurance time for all 4 static tests (all P-values<0.001).
Conclusion Prolonged occupational sitting is associated with reduced core muscle endurance. It may cause relationship between weakened core/trunk muscles and development of specific occupational musculoskeletal dysfunctions such as low back pain.
Psychophysische Kurzzeit- und Langzeiteffekte einer einmaligen Massageanwendung – Ergebnisse einer naturalistischen Studie
Authors: Kiebgis Gabriele , Eggart Michael , Thormählen Christina , Müller-Oerlinghausen Bruno 
Published: July 2018

Zusammenfassung
Einleitung Massagen verschiedenster Art werden zunehmend als komplementäre Therapie bei einer Reihe von Indikationen z. B. auch im geriatrischen oder palliativmedizinischen Bereich oder in der Behandlung Frühgeborener, aber auch in der Prävention von „burn-out“-Syndromen eingesetzt. Kontrollierte Studien belegen den antidepressiven, allgemein entspannenden aber auch analgetischen Effekt. Kaum Daten existieren aber zu den subjektiven psychophysischen Massagewirkungen bei der typischen Klientel üblicher physiotherapeutischer Praxen.
Methoden Wir untersuchten die subjektiven Wirkungen einer einstündigen psychoaktiven Massage („Vasana“) bei 100 Probanden in einer offenen einarmigen Versuchsanordnung mit 3 sukzessiven Messzeitpunkten. Zur Datenerfassung benutzten wir speziell konstruierte Fragebögen und visuelle Analogskalen.
Ergebnisse Die in einer Physiotherapie-Praxis rekrutierte Probandengruppe hatte einen höheren Frauenanteil und wies mehr psychosomatische Beschwerden auf als die deutsche Normalbevölkerung. Unmittelbar nach der Massage stellte sich ein signifikant erhöhtes allgemeines Wohlgefühl im Vergleich zum 14 Tage zuvor erhobenen Ausgangsniveau ein, das nach 14 Tagen nicht mehr beobachtet wurde. Die 13 Dimensionen psychophysischer Befindlichkeit zeigten mehrheitlich signifikante Verbesserungen, z. B. weniger Ängstlichkeit und depressive Stimmung, weniger myofaszialer Schmerz. In einem Drittel der Probanden wurden positive Effekte auch noch nach 14 Tagen dokumentiert, z. B. erhöhte körperliche Aufmerksamkeit, weniger Schlafstörungen. Verschiedene neurobiologische Mechanismen können diese Effekte erklären.
Schlussfolgerungen Massage als eine Form der therapeutischen Berührung sollte verstärkt in moderne multidimensionale Behandlungspläne bei psychosomatischen/psychiatrischen Störungen integriert werden, dies gilt insbesondere auch für die Behandlung chronischer Schmerzzustände. Studien belegen, dass auch in der Geburtsvorbereitung und in der postpartalen Phase Massagen eine wichtige Rolle spielen können.
Influence of Isokinetic Knee Muscle Torque at Different Angular Velocities on Standing Posture Stability in Healthy Adults
Authors: Al-Abdulwahab Sami , Kachanathu Shaji , Alodaibi Faris 
Published: July 2018

Abstract
Objectives Knee muscle groups contribute to proper and safe static and dynamic standing posture stability. The impact of isokinetic knee muscle torque at various angular velocities on standing posture stability is poorly understood. We investigated the influence of isokinetic knee muscle torque at different angular velocities on standing posture stability.
Design Cross-sectional study.
Setting King Saud University Campus labs.
Method The study population was a convenience sample of 30 healthy volunteer college students with a mean age of 20±2 years. Isokinetic concentric peak torque (PT) of knee extensor (Q) and flexor (H) muscle groups as well as the flexor to extensor (H:Q) ratio at three different angular velocities: (60, 180, and 300°/s) were measured, respectively. The postural stability was also measured in both static and dynamic components. The Pearson correlation coefficient was used to examine the association between the different isokinetic components and postural stability (both static and dynamic).
Results The PT of Q and H muscle groups and the H:Q ratio at the 3 different angular velocities were significantly associated with static standing balance with eye open on a firm surface (p<0.033). Moreover, the PT of Q at 60 and 180°/s angular velocities was significantly associated with maximum and endpoint excursion components of dynamic standing balance (p<0.044).
Conclusions The PT of both Q and H muscle groups at different angular velocities are associated with maintenance of standing static balance. PT of Q only is significantly associated with control of dynamic standing balance in the lower angular velocities.