bluuebunny/crossref_metadata_2025_split huggingface dataset:
iTRAQ-based quantitative proteomic analysis of embryonic developmental stages in Amur sturgeon, Acipenser schrenckii
Authors: Jin Shubo , Sun Dajiang , Song Dan , Wang Nianmin , Fu Hongtuo , Ji Feng , Zhang Ying 
Published: April 2018

AbstractThe Amur sturgeon, Acipenser schrenckii, is an important aquaculture species in China with annual production of about 150 thousand tons in 2015. In this study, we investigated the regulatory proteins and pathways affecting embryonic development of Amur sturgeon, by analyzing of the differential proteomes among four embryonic developmental stages using isobaric tags for relative and absolute quantitation (iTRAQ), combined with the analysis of effects of microelements and antioxidants on embryonic development. Seventy-four, 77, and 76 proteins were differentially expressed according to iTRAQ analysis between the fertilized egg and blastula, blastula and neurula, and neurula and heart-beat stages, respectively. GO and KEGG enrichment analyses indicated that Gluconeogenesis, Ribosome and Proteasome were the most enriched pathways, which may promote energy formation, immune system protection and protein synthesis process in A. schrenckii. The measurement of microelements indicated that Mn, Cu and Fe were obtained from their parents or water environment in A. schrenckii, while Zn plays vital roles throughout embryonic development. The dramatically high level of malondialdehyde (MDA) across the embryonic development may be the main reason leading to a low hatching rate in A. schrenckii. This study provides the basis for further proteome analysis of embryonic development in A. schrenckii.
Aloperine Protects Mice against Bleomycin-induced Pulmonary Fibrosis by Attenuating Fibroblast Proliferation and Differentiation
Authors: Yin Wanling , Han Jing , Zhang Zhijun , Han Zaomu , Wang Siyuan 
Published: April 2018

AbstractAloperine is a quinolizidine alkaloid extracted from Sophora alopecuroides. It has been proven to alleviate oxidative stress and effectively promote tumor cell apoptosis in mice. Herein, we investigated whether aloperine could also mediate its protective effects on bleomycin (BLM)-induced pulmonary fibrosis. Pathological staining, western blot, RT-PCR and flow cytometry were used to evaluate the impact of aloperine on the development of pulmonary fibrosis. The effect of aloperine on fibroblast proliferation, differentiation and related signaling pathways were next investigated to demonstrate the underlying mechanisms. In the present report, we showed that aloperine provided protection for mice against BLM-induced pulmonary fibrosis as manifested by the attenuated lung injury and reduced fibrosis along with alleviated fibroblast proliferation and differentiation. Additionally, we provided in vitro evidence revealing that aloperine inhibited cellular proliferation in PDGF-BB-stimulated mouse lung fibroblasts by repressed PI3K/AKT/mTOR signaling and fibroblast to myofibroblast differentiation by repressed TGF-β/Smad signaling. Overall, our data showed that aloperine could protect the mice against BLM-induced pulmonary fibrosis by attenuated fibroblast proliferation and differentiation, which indicated that aloperine may be therapeutically beneficial for IPF patients.
Proteomics Studies on the three Larval Stages of Development and Metamorphosis of Babylonia areolata
Authors: Shen Minghui , Di Guilan , Li Min , Fu Jingqiang , Dai Qi , Miao Xiulian , Huang Miaoqin , You Weiwei , Ke Caihuan 
Published: April 2018

AbstractThe ivory shell, Babylonia areolata, is a commercially important aquaculture species in the southeast coast of mainland China. The middle veliger stage, later veliger stage, and juvenile stage are distinct larval stages in B. areolata development. In this study, we used label-free quantification proteomics analysis of the three developmental stages of B. areolata. We identified a total of 5,583 proteins, of which 1,419 proteins expression level showed significant differential expression. The results of gene ontology enrichment analysis showed that the number of proteins involved in metabolic and cellular processes were the most abundant. Those proteins mostly had functions such as binding, catalytic activity and transporter activity. The results of Kyoto Encyclopedia of Genes and Genomes enrichment analysis showed that the number of proteins involved in the ribosome, carbon metabolism, and lysosome pathways were the most abundant, indicating that protein synthesis and the immune response were active during the three stages of development. This is the first study to use proteomics and real-time PCR to study the early developmental stages of B. areolata, which could provide relevant data on gastropod development. Our results provide insights into the novel aspects of protein function in shell formation, body torsion, changes in feeding habits, attachment and metamorphosis, immune-related activities in B. areolata larvae.
Population structure of Environmental and Clinical Legionella pneumophila isolates in Catalonia
Authors: Quero Sara , Párraga-Niño Noemí , Barrabeig Irene , Sala Maria Rosa , Pedro-Botet M. Luisa , Monsó Eduard , Jané Mireia , Sabrià Miquel , Garcia-Núñez Marian 
Published: April 2018

AbstractLegionella is the causative agent of Legionnaires’ disease (LD). In Spain, Catalonia is the region with the highest incidence of LD cases. The characterisation of clinical and environmental isolates using molecular epidemiology techniques provides epidemiological data for a specific geographic region and makes it possible to carry out phylogenetic and population-based analyses. The aim of this study was to describe and compare environmental and clinical isolates of Legionella pneumophila in Catalonia using sequence-based typing and monoclonal antibody subgrouping. A total of 528 isolates were characterised. For data analysis, the isolates were filtered to reduce redundancies, and 266 isolates (109 clinical and 157 environmental) were finally included. Thirty-two per cent of the clinical isolates were ST23, ST37 and ST1 while 40% of the environmental isolates were ST284 and ST1. Although the index of diversity was higher in clinical than in environmental ST isolates, we observed that clinical STs were similar to those recorded in other regions but that environmental STs were more confined to particular study areas. This observation supports the idea that only certain STs trigger cases or outbreaks in humans. Therefore, comparison of the genomes of clinical and environmental isolates could provide important information about the traits that favour infection or environmental persistence.
Measurements of particle backscatter, extinction, and lidar ratio at 1064 nm with the rotational raman method in Polly-XT
Authors: Engelmann Ronny , Haarig Moritz , Baars Holger , Ansmann Albert , Kottas Michael , Marinou Eleni 
Published: April 2018

We replaced a 1064-nm interference filter of a Polly-XT lidar system by a 1058-nm filter to observe pure rotational Raman backscattering from atmospheric Nitrogen and Oxygen. Polly-XT is compact Raman lidar with a Nd:YAG laser (20 Hz, 200 mJ at 1064 nm) and a 30-cm telescope mirror which applies photomultipliers in photoncounting mode.
We present the first measured signals at 1058 nm and the derived extinction profile from measurements aboard RV Polarstern and in Leipzig. In combination with another Polly-XT system we could also derive particle backscatter and lidar ratio profiles at 1064 nm.
Retrieval of atmospheric backscatter and extinction profiles with the aladin airborne demonstrator (A2D)
Authors: Geiss Alexander , Marksteiner Uwe , Lux Oliver , Lemmerz Christian , Reitebuch Oliver , Kanitz Thomas , Straume-Lindner Anne Grete 
Published: April 2018

By the end of 2017, the European Space Agency (ESA) will launch the Atmospheric laser Doppler instrument (ALADIN), a direct detection Doppler wind lidar operating at 355 nm. An important tool for the validation and optimization of ALADIN’s hardware and data processors for wind retrievals with real atmospheric signals is the ALADIN airborne demonstrator A2D. In order to be able to validate and test aerosol retrieval algorithms from ALADIN, an algorithm for the retrieval of atmospheric backscatter and extinction profiles from A2D is necessary.
The A2D is utilizing a direct detection scheme by using a dual Fabry-Pérot interferometer to measure molecular Rayleigh signals and a Fizeau interferometer to measure aerosol Mie returns. Signals are captured by accumulation charge coupled devices (ACCD). These specifications make different steps in the signal preprocessing necessary.
In this paper, the required steps to retrieve aerosol optical products, i. e. particle backscatter coefficient βp, particle extinction coefficient αp and lidar ratio Sp from A2D raw signals are described.
Evaluierung der mundgesundheitsbezogenen Lebensqualität bei seltenen Erkrankungen mit oraler Beteiligung
Authors: Hanisch Marcel , Jung Susanne , Kleinheinz Johannes 
Published: April 2018

Zusammenfassung
Ziel der Studie Um die medizinische Versorgung von Menschen mit Seltenen Erkrankungen (SE) zu verbessern wurde 2013 durch das Bundesministerium für Gesundheit ein „Nationaler Aktionsplan für Menschen mit Seltenen Erkrankungen“ (NAMSE) ins Leben gerufen. Ziel des NAMSE ist dabei, eine medizinisch adäquate Versorgung für die Betroffenen aufzubauen sowie Informationen über die aktuelle Versorgungssituation von SE zu gewinnen. Vor diesem Hintergrund sollen Informationen zur die mundgesundheitsbezogenen Lebensqualität (OHRQoL) bei Menschen mit SE und oraler Beteiligung gewonnen werden.
Methodik Unter dem Hintergrund des NAMSE wurde eine Spezialsprechstunde für SE mit oraler Beteiligung eingerichtet. Mittels dem standardisiertem OHIP-14 Fragebogen wurden Informationen zur OHRQoL sowie allgemeine Informationen zur Erkrankung gewonnen.
Ergebnisse Insgesamt wurden 50 Patienten in der Sprechstunde behandelt. Diese nahmen durchschnittlich eine einfache Anfahrtsstrecke von 143 km auf sich. Im Mittel mussten die Betroffenen 5 Jahre auf die finale Diagnose ihrer SE warten. Die Betroffenen weisen einen durchschnittlichen OHIP-14 Gesamt-Score von 26,4 auf.
Schlußfolgerung Der lange Anfahrtsweg zeigt, welchen Aufwand Patienten insbesondere bei mehrfachen Vorstellungen auf sich nehmen müssen. Hieraus entsteht die Forderung, künftig flächendeckend Spezialsprechstunden für SE mit oraler Beteiligung aufzubauen, wie dies auch im NAMSE gefordert wird. Der Vergleich mit den wenigen vorhandenen Daten lässt auf eine deutlich schlechtere OHRQoL in unserem Patientenkollektiv schließen. Seltene Erkrankungen mit oraler Beteiligung scheinen insgesamt von einer früheren Diagnose der Erkrankung zu profitieren, als SE ohne orale Beteiligung.
Psychosoziale Beratung in einem gemeindepsychiatrischen Zentrum – eine qualitative Studie über die Erfahrungen der Nutzer
Authors: Schwarz Julian , Berghöfer Anne , Brückner Burkhart , Birker Thomas , Stöckigt Barbara 
Published: April 2018

Zusammenfassung
Ziel Analyse der Erfahrungen von Nutzern einer innovativen, rechtskreisübergreifenden psychosozialen Beratungsstelle.
Methode Neun Einzelinterviews wurden qualitativ-inhaltsanalytisch ausgewertet.
Ergebnisse Das Angebot stärkte psychosoziale Ressourcen und die Autonomie der Nutzer. Positiv wurde die aufsuchende Beratung bewertet, kritisiert wurde die begrenzte Sichtbarkeit des Angebots.
Schlussfolgerungen Eine rechtskreisübergreifende Beratungsstelle kann Hilfesuchende in Krisen auffangen und passgenau gemeindepsychiatrische oder psychosoziale Angebote vermitteln.
Wrist Arthroscopy in Juvenile Idiopathic Arthritis: A Review of Current Literature and Future Implications
Authors: Vermaak P. , Southwood T. , Lindau T. , Jester A. , Oestreich K. 
Published: April 2018

Introduction Juvenile Idiopathic Arthritis (JIA) is the most common rheumatological condition in children and frequently affects the wrist. The roles for wrist arthroscopy and arthroscopic synovectomy (AS) in JIA are unclear. Our aim was to find the current evidence supporting its use.
Methods Systematic literature review of relevant publications from 1990 to present in the Cochrane Library, Clinical Knowledge Summaries, DynaMed, PEMSoft, NICE Guidance, MEDLINE, EMBASE, and PubMed.
Results We found no publications detailing the use of arthroscopy or AS specifically in patients with JIA involving the wrist. There is evidence that AS reduces pain, improves function, and induces remission in patients with rheumatoid arthritis resistant to medical management.
Discussion and Conclusion Although there is paucity in evidence for the use of AS in the wrists of patients with JIA, studies suggest it to be safe and effective, and could be applied to patients with refractive JIA. It is possible that early identification of patients suffering from JIA with extensive joint destruction and little symptoms could benefit from AS, delaying joint destruction and preserving function.
The Impact of Hypoxemia on the Development of Retinopathy of Prematurity in Infants Less Than 29 Weeks of Gestation
Authors: Afshar Narges , Baier Ronald , Tagin Mohamed , Cabo Cecilia , Minski John , Elsayed Yasser 
Published: April 2018

Objective To study the impact of cumulative exposure to hypoxemia on the development of retinopathy of prematurity (ROP) in preterm infants less than 29 weeks' gestation.
Study Design This is a retrospective analysis of the effect of cumulative exposure to hypoxemia during the first 10 weeks of life in preterm infants <29 weeks' gestation. Cumulative time spent at various levels of oxygen saturation was calculated by converting the daily percentage of time to minutes per day. Cumulative exposure to hypoxemia (cT<80 or oxygen saturation <80%) was calculated weekly and compared between outcomes. The primary outcome was the development of ROP requiring treatment.
Results Cumulative hypoxemia exposure was significantly associated with ROP requiring treatment. When adjusted for other neonatal morbidities, only gestation was consistently associated with ROP requiring treatment.
Conclusion Cumulative exposure to hypoxemia in the first few weeks was not associated with ROP or treatment of ROP after adjustment for confounders.
DRUG-NEM: Optimizing drug combinations using single-cell perturbation response to account for intratumoral heterogeneity
Authors: Anchang Benedict , Davis Kara L. , Fienberg Harris G. , Williamson Brian D. , Bendall Sean C. , Karacosta Loukia G. , Tibshirani Robert , Nolan Garry P. , Plevritis Sylvia K. 
Published: April 2018

Significance
Single-cell high-throughput technologies enable the ability to identify combination cancer therapies that account for intratumoral heterogeneity, a phenomenon that has been shown to influence the effectiveness of cancer treatment. We developed and applied an approach that identifies top-ranking drug combinations based on the single-cell perturbation response when an individual tumor sample is screened against a panel of single drugs. This approach optimizes drug combinations by choosing the minimum number of drugs that produce the maximal intracellular desired effects for an individual sample.
A new perspective on the Luxembourgish genitive
Authors: Döhmer Caroline 
Published: April 2018

Abstract
This paper deals with genitive constructions in the Luxembourgish language. It is often assumed that the Luxembourgish genitive has only survived in idioms and archaic structures (Russ 1996: 74, Schanen & Zimmer 2012: 64), but I will investigate this claim in more detail. Based on a qualitative corpus analysis of spoken and written Luxembourgish (over 62 million tokens), different constructions will be systematically compiled and analyzed. Due to the rising interest in morphosyntactic variation and the improved data situation, this descriptive analysis investigates the current status of the genitive within a single system of grammar, i.e. Luxembourgish. The paper explores six main areas that are relevant for Luxembourgish genitives: attributes, genitive complements of verbs and adjectives, prepositions, adverbials, family names, and partitives. The structures obtained from the corpus will then be discussed from a formal and a functional perspective in order to illustrate Luxembourgish case typology. As the data shows, the genitive behaves differently according to the different areas that have been selected beforehand. On the one hand, genitives can be found in lexicalized structures, i.e. adverbials. On the other hand, different genitives occasionally appear as verb or adjective complements. This phenomenon is partly caused by the expansion of the Luxembourgish language into the written domain. Another main finding is that Luxembourgish makes full use of an independent set of partitive pronouns and determiners that have been derived from former genitives.
On the motivation of genitive‑s omission in Contemporary German
Authors: Zimmer Christian 
Published: April 2018

Abstract
In Contemporary German strong, masculine and neuter nouns in genitive phrases either take a suffix (‑s or -es) or remain uninflected for case (e.g. Nutzung d-es Internet‑s vs. Nutzung d-es Internet-Ø, ‘use of the-gen.sg internet-gen.sg/Ø’). This paper is concerned with a corpus-based description of this variation on the basis of which an explanation of the phenomenon is given. Using data taken from synchronic and diachronic corpora (
DECOW2012
and Deutsches Textarchiv) and psycholinguistic evidence from a self-paced reading task it is shown that there is a functional motivation for the fact that a well-delimited group of nouns (i.e. peripheral nouns, such as loan words, abbreviations and proper names) tend to remain uninflected for case whilst prototypical German nouns are almost always inflected for genitive case. This variation results from the competition between two motivations: syntactic agreement on the one hand and a hitherto not very well described factor called morphological schema constancy on the other hand. The latter refers to the maintenance of word form stability e.g. through the avoidance of inflectional suffixes. However, these motivations are weighted differently depending on the nature of the noun in question: Since syntactic agreement is still important for the grammatical system, prototypical nouns are unaffected by ‑s omission. Thus, in general, the genitive singular resists the general German(ic) tendency towards a reduction of nominal case markers so far.
From genitive suffix to linking element
Authors: Kopf Kristin 
Published: April 2018

Abstract
Present-day German uses two formally different patterns of compounding in N+N compounds. The first combines bare stems (e.g. Tisch+decke ‘tablecloth’) while the second contains an intervening linking element (le) as in Geburt‑s-ort ‘birth-le-place’. The linked compounding type developed in Early New High German (1350–1650) from phrasal constructions by reanalyzing genitive attributes as first constituents of compounds. The present paper uses corpus data to explore three key stages in this development: In the initial stage, it shows how prenominal non-specific genitive constructions lent themselves to reanalysis due to their functional overlap and formal similarity. Additionally, compounds seem to have replaced not only prenominal genitives, but also structurally different postnominal genitives. In the second stage, the new compounding pattern increases in productivity between 1500 and 1710, especially compared to the older pattern without linking elements. The last stage pertains to changes in spelling practice. It shows that linked compounds were written separately in the beginning. Their gradual graphematic integration into directly connected words was reversed by a century of hyphenation (1650–1750). This is strikingly different from present-day spelling practice and shows that the linked pattern was still perceived as marked.
From genitive inflection to possessive marker?
Authors: Ackermann Tanja 
Published: April 2018

Abstract
This paper deals with the status of the s-marker with personal names in German from a diachronic perspective. On the basis of corpus data it is argued that the deflexion of personal names in genitive phrases is much more advanced than it has been claimed in the literature: only the invariant marker ‑s occurs frequently, namely in constructions of the type Hildes Wohnung ‘Hilde’s flat’. Thus, it becomes obvious that such constructions, which are less widely studied than their English or Swedish counterparts, play a special role. It will be argued that an interpretation of the ‑s in these adnominal possessive constructions as a kind of prenominal genitive use fails. Given this, a comparison with the development of possessive ‑s in the history of English reveals that there are striking similarities with the developments in German.
Genitives and proper name compounds in German
Authors: Schlücker Barbara 
Published: April 2018

Abstract
This paper deals with an issue related to the syntax-morphology interface, namely the comparison of proper name compounds and genitives in German. It has been claimed in the literature that proper name compounds have recently increased in use at the expense of the genitive. For this reason, proper name compounds are sometimes considered as competitors to the genitive. A prerequisite for this assumption is that both constructions are equivalent with respect to meaning and function. The paper examines the relevant properties of both constructions. It is argued that there is no true meaning equivalence between proper name compounds and genitives. However, specific subtypes of proper name compounds and genitives that exhibit a substantial amount of semantic overlap are identified. It is discussed under which circumstances they can be used interchangeably.
Mirativity and egophoricity in Kurtöp
Authors: Hyslop Gwendolyn 
Published: April 2018

Abstract
The terms ‘mirative’ and ‘egophoric’ have often been employed to describe various epistemic contrasts which, on the surface, appear to be quite similar. Kurtöp, a Tibeto-Burman language of Northeastern Bhutan, contrasts both mirativity and egophoricity, providing evidence that they are two separate categories. Mirativity in Kurtöp is a pervasive feature of the grammar; it occurs in imperfective and perfective aspect, in addition to being encoded in affirmative and negative forms of existential and equational copulas. In statements, the mirative is used to encode that knowledge was new or unexpected to the speaker while non-mirative forms encode old knowledge. In questions, only non-mirative forms are used. In contrast to mirative marking, egophoricity is marked in perfective aspect only. An egophoric form encodes that the speaker has intimate knowledge of an event or intention of an event; a non-egophoric form does not encode this personal knowledge. Unusually, egophoric marking in Kurtöp includes a distinction as to whether knowledge is exclusive to the speaker, or is thought to be shared. Mirative and egophoric forms have particular tendencies to co-occur with certain subjects, especially in elicitation. However, when we look within natural discourse, these categories are complex, with subtler meanings than basic elicitation would suggest.