bluuebunny/crossref_metadata_2025_split huggingface dataset:
Perineal schwannoma (case report and review)
Authors: Bragina A. S. , Anosov I. S. , Khryukin R. Yu. , Maynovskaya O. A. 
Published: November 2024

Schwannoma (also known as neurilemmoma) is a type of benign tumor that originates from the myelin sheath around of peripheral nerves. It is composed of schwann cells, which are specialized cells that help to insulate nerve fibers. Schwannomas are relatively rare, accounting for only 5% of all benign tumors in the perineal region. They are usually asymptomatic and are only detected when they come into contact with other organs or structures, causing symptoms such as compression. Preoperative diagnosis of schwannoma is challenging, as it is often difficult to distinguish it from other types of tumors in the same area. The final diagnosis can only be confirmed by intraoperative histology.The method of choice for benign neurilemmomas is complete removal, as they continue growth after resection. Removing part of the tumor may be an option in cases where removing the neoplasm en bloc could damage adjacent structures, such as anal sphincter, levators, rectum, vagina and urethra.Clinical case: twenty-five years old female, had complaints of a tumor in the left buttock area and discomfort in a sitting position for 3 years. Pelvic MRI with intravenous contrast revealed a tumor 6.0 × 5.1 × 9.0 cm in the left ischial region. Surgical removal of the tumor was performed.
Role of fluorescence navigation with indocyanine green during lateral pelvic lymphodissection in the treatment of rectal cancer (meta-analysis)
Authors: Tishkevich I. S. , Alekseev M. V. , Khomyakov E. A. , Rybakov E. G. 
Published: November 2024

INTRODUCTION: intraoperative fluorescence navigation with indocyanine green (ICG) allows visualizing possible affected lymph nodes, which hypothetically provides more precise lateral pelvic node dissection (LPND).AIM: to compare immediate results of lateral pelvic node dissection (LPND) combined with fluorescence navigation with ICG and conventional LPND for the treatment of metastatic lesions of lateral pelvic lymph nodes in patients with rectal cancer.PATIENTS AND METHODS: search was performed in the PubMed library (National Library of Medicine, Bethesda, MD, USA) with keywords “indocyanine green,” “ICG“, ”lymph nodes,” “lateral lymph node dissection,” and “rectal cancer” in various combinations. Four non-randomized studies were included in meta-analysis, three retrospective and one prospective, on the outcomes of ICG use during LPND in patients with rectal cancer in the meta-analysis.RESULTS: significantly more lymph nodes were harvested in LPND + ICG group (MD = 4.5; 95% CI: 3.0–5.9; p < 0.00001). The operation time was longer in the ICG group (MD = 32.5; 95% CI: 2.8–62.3; p = 0.03). Intraoperative blood loss was higher in conventional LPND group (MD = −52.6; 95% CI: −89.8 — −15.3; p = 0.006). Hospital stay was significantly shorter in ICG group (MD = −1.2; 95% CI: −2.0 — −0.4; p = 0.003). Patients who underwent standard LPND were significantly more likely to have postoperative urinary retention (OR = 0.4; 95% CI: 0.1–0.9; p = 0.03).CONCLUSION: fluorescence navigation with ICG improves early results of LPND. Further accumulation of experience with dynamic follow-up of patients is crucial.
Impact of neoadjuvant chemotargeted therapy in patients with colorectal cancer and synchronous liver metastases in perioperative period
Authors: Yunusova N. V. , Dobrodeev A. Yu. , Kostromitsky D. N. , Afanasyev S. G. , Tarasova A. S. , Ermolenko R. V. , Babyshkina N. N. , Dronova T. A. , Ponomareva A. A. , Larionova I. V. 
Published: November 2024

AIM: assess the impact of neoadjuvant chemotargeted therapy in patients with colorectal cancer and synchronous liver metastases in perioperative period.PATIENTS AND METHODS: a pilot prospective study included 30 patients with colorectal cancer and synchronous liver metastases (mCRC). The combined treatment included 3 cycles of neoadjuvant FOLFOXIRI chemotherapy with the addition of targeted agents: cetuximab (24 patients with wtKRAS) and bevacizumab (6 patients with mtKRAS) followed by radical surgery.RESULTS: the clinical and radiological response of colorectal cancer liver metastases to neoadjuvant chemotherapy (NACT) was complete in 4 (13.3%) patients and partial in 26 (86.7%) patients. Partial response to NACT in the primary tumor occurred in all patients. Adverse events of NACT were detected in 12 (40%) patients, 1 (3.3%) of them produced grade III toxicity. All patients underwent radical surgery (R0) 3–4 weeks after NACT, 28 (93.3%) of them underwent simultaneous colorectal and liver resection. Postoperative complications occurred in 21 (70%) patients, including grade I and grade IIIa complications (according to Сlavien-Dindo classification) — 22 (73.3%) and 2 (6.7%), respectively. Histology revealed pathologic complete response (pCR) of liver metastases in 1 (3.6%) case and pathological grade 3 regression of the primary tumor (TRG3, Mandard A.M.) in 23 (76.7%) patients. Two (6.7%) patients with complete clinical and radiological response of liver metastases, who did not undergo liver resection, had no evidence of disease progression 12 months after the treatment.CONCLUSION: in mCRC with synchronous liver metastases, NACT according to the FOLFOXIRI regimen in combination with targeted agents with a moderate toxicity profile provide significant carcinocidal effect without having a negative impact in the perioperative period. The study is ongoing to analyze 2-year disease-free and overall survival of patients.
Does a surgical access for rectal cancer in older patients affect postoperative morbidity and oncological safety?
Authors: Polovinkin V. V. , Shiraliev R. M.-A. , Ermakov E. A. , Doronin N. V. , Balyan A. S. , Khalafyan A. A. , Karmaseva V. S. 
Published: November 2024

AIM: to evaluate the effect of a surgical access on postoperative morbidity and oncological safety in older patients.PATIENTS AND METHODS: a multicenter retrospective study included 179 patients aged 60–74 years who underwent surgery for middle and low rectal cancer (T1-3N0-N2bM0) in 2021-2023. The patients were divided into 3 groups: the first ones underwent robotic (ROB) surgery (n = 62), the second — laparoscopic (LAP) surgery (n = 55), and the third — open (OPEN) surgery (n = 62). Parameters of pre-, intra-, and postoperative periods and histopathological findings were evaluated. Neural network modeling was used to predict anastomotic leakage (AL).RESULTS: the blood loss was 150 (100; 200) ml with OPEN versus 100 (50; 100) with ROB and LAP, the operation time was 255 (210; 300) min with ROB versus 180 (150; 240) min with LAP and 140 (120; 150) min with OPEN. In ROB and LAP groups, anastomoses were formed in 61 (98.4%) and 54 (98.2%) cases compared with 45 (72.6%) cases in the OPEN group (p = 0.00001). The splenic flexure was mobilized in 54 (98.2%) cases in the LAP group, 55 (88.7%) cases in the OPEN group, and 50 (80.7%) cases in the ROB group (p = 0.01). Conversion rates were 10.9% (6/55) and 1.6% (1/62) in LAP and ROB groups, respectively (p = 0.00001). Postoperative complications in the OPEN group occurred in 48 (77.4%) cases compared with 31 (50%) and 12 (21.8%) in ROB and LAP groups (p = 0.02). Inflammatory complications predominated in the OPEN group. Distal and lateral margins, the quality of mesorectal excision, and the number of examined and affected lymph nodes did not differ. However, the good quality of mesorectal excision prevailed in ROB and OPEN groups, where as the satisfactory quality was more common in the LAP group. The most important predictors of AL were American Society of Anesthesiologists (ASA) physical status II, neoadjuvant chemoradiotherapy, stage I and IIa cancer, end-to-end anastomosis, Charlson Comorbidity Index scores of 3–4, and surgeon’s experience (20–40 operations for rectal cancer per year). The least important predictors were the level of mesenteric vessel ligation and the access.CONCLUSIONS: the surgical access does not affect the AL rate. The histology revealed that all the 3 approaches ensure compliance with principles of oncological safety. Compared with open surgery, robotic and laparoscopic surgery result in less blood loss and faster recovery of intestinal function; however, the operation time increases.
What lies ahead? Consumer expectations and wishes for organic animal farming: analyzing improvements to boost confidence in organic livestock farming
Authors: Bayer Elisa , Kühl Sarah 
Published: November 2024

IntroductionTo meet the policy target of increasing the share of organic farming in the EU to 25% by 2030, it is also essential to increase consumer demand for organic products. For many consumers, animal welfare is one of the main reasons to buy organics. Trust is inherently important when purchasing organic food, as most of the standards on which organic farming relies, such as more space, cannot be verified by the consumer. Consumer trust in organic animal farming is therefore an important factor in the expansion of the market share of organic products. The aim of this study is to identify specific trust-building “changes” in organic livestock husbandry to strengthen consumer trust in the long term.MethodA total of eight regulations or practices, e.g. early cow-calf separation or regulations on the use of antibiotics, were assessed in terms of consumer awareness. Further described changes to these practices or regulations were ranked regarding their potential to improve trust in organic animal husbandry.ResultsThe results show that most of the regulations and practices mentioned were not known to consumers, confirming a low level of consumer knowledge about specific aspects of organic farming. All eight possible changes would increase consumer trust, at least somewhat.DiscussionThe ranking shows that, in particular, the practice of dam rearing and an even stronger limitation of flock sizes for poultry have the highest potential to increase consumer trust in organic animal husbandry and can therefore be recommended as important strategies to meet consumer demands in the future.
Geometrically modified bovine pericardium membrane promotes the expression of molecules targeted for a faster integration and vascularization process
Authors: Morgante Olga , Della Rocca Ylenia , Marconi Guya Diletta , Mazzone Antonella , Cavalcanti Marcos F. X. B. , Trubiani Oriana , Diomede Francesca , Pizzicannella Jacopo 
Published: November 2024

IntroductionIn recent years, advancements in technology and the refinement of engineering techniques have facilitated the development of tissue engineering, placing particular emphasis on the use of 3D-biomaterials with several structural and chemical geometric features. In particular, increasing information on biomaterial geometric surfaces has allowed for a better understanding of tissue regenerative processes. In the present study a comparison between BioRipar®, bovine pericardium membrane, modified with micrometric roundish regular open pores (BioR-Ps) and BioRipar® without pores (BioR-NPs) has been investigated.MethodsThe expression of adhesion molecules such as: fibronectin, vimentin, focal adhesion kinase (FAK), integrin 1β, integrin α5, E-cadherin, and molecules involved in neovascularization processes such as: vascular endothelial growth factor (VEGF) and vascular endothelial growth factor receptor (VEGF-R) were evaluated in an in vitro model containing primary culture of human periodontal ligament stem cells (hPDLSCs) through multiparametric analysis.ResultsThe results indicated a markedly significant expression of all the abovementioned molecules in hPDLSCs cultured withBioR-Ps compared to hPDLSCs cultured with BioR-NPs. Scanning electron microscopy analysis indicated a marked interaction between the cells and the substrate, particularly evident in the proximity of open pores in the hPDLSCs cultured on the BioR-P surface compared to hPDLSCs cultured on the BioR-NP surface. Thus, the presence of micrometric open pores on the scaffold stimulates the proliferation potential of cells apart from their adhesion ability on the patch, particularly near the poresDiscussionExpression of angiogenic molecules strengthened the performance of the modified BioR-Ps. During synthesis, 3D-biomaterial micrometric open-pores enable better bonding between cells and materials, increasing contact area and promoting cellular molecular signals in biomaterial-guided tissue engineering.
Application of 3D printing in the treatment of diabetic foot ulcers: current status and new insights
Authors: Li Xinrui , Ai Xin , Wang Bo , Luo Mengqian , Miyamoto Akira , Kuchay Mohammad Shafi , Feng Dechao , Zhang Chi 
Published: November 2024

Background and AimsDiabetic foot ulcers (DFUs) are a serious complication of diabetes mellitus (DM), affecting around 25% of individuals with DM. Primary treatment of a DFU involves wound off-loading, surgical debridement, dressings to provide a moist wound environment, vascular assessment, and appropriate antibiotics through a multidisciplinary approach. Three-dimensional (3D) printing technology is considered an innovative tool for the management of DFUs. The utilization of 3D printing technology in the treatment of DFU involves the modernization of traditional methods and the exploration of new techniques. This review discusses recent advancements in 3D printing technology for the application of DFU care, and the development of personalized interventions for the treatment of DFUs.MethodsWe searched the electronic database for the years 2019–2024. Studies related to the use of 3D printing technology in Diabetic foot were included.ResultsA total of 25 identified articles based on database search and citation network analysis. After removing duplicates, 18 articles remained, and three articles that did not meet the inclusion criteria were removed after reading the title/abstract. A total of 97 relevant articles were included during the reading of references. In total, 112 articles were included.Conclusion3D printing technology offers unparalleled advantages, particularly in the realm of personalized treatment. The amalgamation of traditional treatment methods with 3D printing has yielded favorable outcomes in decelerating the progression of DFUs and facilitating wound healing. However, there is a limited body of research regarding the utilization of 3D printing technology in the domain of DFUs.
Saliva NIR spectroscopy and Aquaphotomics: a novel diagnostic approach to Paratuberculosis in dairy cattle
Authors: Behdad Saba , Pakdel Abbas , Massudi Reza 
Published: November 2024

IntroductionParatuberculosis is a granulomatous intestinal infection that affects ruminant animals worldwide. The disease is often detected when most animals are already infected due to the long incubation period and the high transmissibility of the infectious agent. The lack of a comprehensive method to diagnose Paratuberculosis is a global challenge. Therefore, a non-destructive, fast, and cost-effective diagnostic method for early detection of Paratuberculosis is crucial.MethodsNear-infrared spectroscopy (NIRS) and Aquaphotomics have the potential to diagnose the disease by detecting changes in biological fluids. This study aimed to investigate the diagnostic ability of NIRS and Aquaphotomics for Paratuberculosis in dairy cattle by monitoring and data mining of saliva. The diagnostic models were developed according to saliva spectra of dairy cattle in the NIR range and 12 water absorbance bands from 100 to 200 days after calving in two groups: positive and negative, based on the same results of seven ELISA tests of blood plasma, as a reference test.ResultsBoth NIRS and Aquaphotomics methods had high diagnostic accuracy. Using QDA and SVM models, 99% total accuracy, 98% sensitivity, and 100% specificity were achieved in internal validation. The total accuracy in external validation was 90%. This study presents two novel approaches to diagnosing Paratuberculosis in dairy cattle using saliva.DiscussionThe study found that changes in water absorbance spectral patterns of saliva caused by complex physiological changes, such as the amount of antibody related to Paratuberculosis in dairy cattle as biomarkers, are crucial in detecting Paratuberculosis using NIRS and Aquaphotomics.
Using a computational cognitive model to simulate the effects of personal and social network experiences on seasonal influenza vaccination decisions
Authors: Walsh Matthew M. , Parker Andrew M. , Vardavas Raffaele , Nowak Sarah A. , Kennedy David P. , Gidengil Courtney A. 
Published: November 2024

IntroductionSeasonal influenza poses significant societal costs, including illness, mortality, and reduced work productivity. Vaccination remains the most effective strategy for preventing the disease, yet vaccination rates in the United States fall below 50% for adults. Understanding the factors influencing vaccination decisions is crucial for designing interventions to improve uptake. This study investigates how personal experiences and the experiences of social contacts affect individual decisions to get vaccinated against influenza.MethodsA multi-year longitudinal survey study was conducted to examine the impact of personal and social network experiences on vaccination decisions. Participants' vaccination behaviors and experiences with influenza were tracked over time. To model these influences, we developed a memory-based vaccination decision model using the Adaptive Control of Thought – Rational (ACT-R) integrated cognitive architecture, which incorporates cognitive processes associated with memory and decision-making.ResultsThe survey results demonstrated that both personal experiences with influenza and the experiences of close social contacts significantly influenced vaccination decisions. The memory-based model, built within the ACT-R framework, effectively captured these effects, providing a computational representation of how personal and social factors contribute to vaccination behaviors.DiscussionThe findings suggest that personal and social experiences play a critical role in shaping vaccination decisions, which can inform the development of targeted interventions to increase vaccination uptake. By incorporating cognitive processes into the model, we identified potential strategies to enhance vaccine promotion efforts, such as recalling past experiences with illness to motivate individuals to get vaccinated.
Automated lung segmentation on chest MRI in children with cystic fibrosis
Authors: Ringwald Friedemann G. , Wucherpfennig Lena , Hagen Niclas , Mücke Jonas , Kaletta Sebastian , Eichinger Monika , Stahl Mirjam , Triphan Simon M. F. , Leutz-Schmidt Patricia , Gestewitz Sonja , Graeber Simon Y. , Kauczor Hans-Ulrich , Alrajab Abdulsattar , Schenk Jens-Peter , Sommerburg Olaf , Mall Marcus A. , Knaup Petra , Wielpütz Mark O. , Eisenmann Urs 
Published: November 2024

IntroductionSegmentation of lung structures in medical imaging is crucial for the application of automated post-processing steps on lung diseases like cystic fibrosis (CF). Recently, machine learning methods, particularly neural networks, have demonstrated remarkable improvements, often outperforming conventional segmentation methods. Nonetheless, challenges still remain when attempting to segment various imaging modalities and diseases, especially when the visual characteristics of pathologic findings significantly deviate from healthy tissue.MethodsOur study focuses on imaging of pediatric CF patients [mean age, standard deviation (7.50 ± 4.6)], utilizing deep learning-based methods for automated lung segmentation from chest magnetic resonance imaging (MRI). A total of 165 standardized annual surveillance MRI scans from 84 patients with CF were segmented using the nnU-Net framework. Patient cases represented a range of disease severities and ages. The nnU-Net was trained and evaluated on three MRI sequences (BLADE, VIBE, and HASTE), which are highly relevant for the evaluation of CF induced lung changes. We utilized 40 cases for training per sequence, and tested with 15 cases per sequence, using the Sørensen-Dice-Score, Pearson’s correlation coefficient (r), a segmentation questionnaire, and slice-based analysis.ResultsThe results demonstrated a high level of segmentation performance across all sequences, with only minor differences observed in the mean Dice coefficient: BLADE (0.96 ± 0.05), VIBE (0.96 ± 0.04), and HASTE (0.95 ± 0.05). Additionally, the segmentation quality was consistent across different disease severities, patient ages, and sizes. Manual evaluation identified specific challenges, such as incomplete segmentations near the diaphragm and dorsal regions. Validation on a separate, external dataset of nine toddlers (2–24 months) demonstrated generalizability of the trained model achieving a Dice coefficient of 0.85 ± 0.03.Discussion and conclusionOverall, our study demonstrates the feasibility and effectiveness of using nnU-Net for automated segmentation of lung halves in pediatric CF patients, showing promising directions for advanced image analysis techniques to assist in clinical decision-making and monitoring of CF lung disease progression. Despite these achievements, further improvements are needed to address specific segmentation challenges and enhance generalizability.
EGFR bypass activation mediates acquired resistance to regorafenib in hepatocellular carcinoma
Authors: Hu Lili , Shi Weiwei , Liu Kua , Ma Ding , Xin Qilei , Wang Zhongxia , Cao Yin , Zhang Guang 
Published: November 2024

BackgroundRegorafenib, a tyrosine kinase inhibitor (TKI), is used in the treatment of unresectable hepatocellular carcinoma (HCC). However, the occurrence of acquired resistance limits its antitumor efficacy. While multiple studies have highlighted the crucial role of bypass activation in acquired TKI resistance, few have focused on bypass activation in regorafenib resistance in HCC.MethodsHigh-throughput proteomics was used to identify differential proteins associated with bypass activation between acquired regorafenib-resistant cells and parental cells. The ability of epidermal growth factor receptor (EGFR) bypass inhibition to reverse resistance was evaluated both in vitro and in vivo using direct microscopic observation, the CCK-8 assay, colony formation assay, Annexin V-FITC/propidium iodide double staining, cell cycle analysis, western blotting, and a xenograft model.ResultsThe expression of EGFR, a member of the receptor tyrosine kinase (RTK) family, was significantly increased in acquired regorafenib-resistant HCC cells compared with parental cells. Pharmacological inhibition of EGFR with gefitinib restored the sensitivity of regorafenib-resistant HCC cells to regorafenib. In a xenograft mouse model, gefitinib sensitized resistant tumors to regorafenib. Additionally, levels of RAS, RAF, and P-ERK1/2, components of the downstream EGFR signaling pathway, were positively associated with EGFR expression.ConclusionEGFR overexpression promotes acquired resistance to regorafenib through RAS/RAF/ERK bypass activation in HCC. Inhibition of EGFR restores sensitivity to regorafenib, and the combination of gefitinib and regorafenib demonstrates significant antitumor efficacy both in vivo and in vitro. These findings suggest that this combination could be a potential strategy for patients with advanced HCC.