bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rapamycin ameliorates psoriasis by regulating the expression and methylation levels of tropomyosin viaERK1/2 andmTORpathways in vitro and in vivo
Authors: Gao Minhong , Si Xiaoqing 
Published: July 2018

AbstractPsoriasis is a chronic inflammatory disease, affecting more than millions of people in the world. Recently, themTORinhibitor rapamycin (RAPA) was reported to be involved in the pathogenesis of psoriasis. However, the underlying mechanism remains unclear. Haematoxylin and eosin staining was used to examine the effects ofRAPAon inflammatory level of lesional tissues from patients with psoriasis and animal models. Quantitative real‐timePCR, immunohistochemistry and western blot assay were performed to assess the effects ofRAPAon tropomyosins (TPMs) expression in patients with psoriasis, cell models and animal models. Phalloidin staining was used to assess theRAPAeffects on cell skeleton. The effects ofRAPAon cell proliferation and cell cycle were detected byCCK‐8 assay, EdU staining and flow cytometry. Methylation status ofTPMs was analysed by methylation‐specificPCR. The expression ofTPM1 andTPM2 was significantly downregulated, while their methylation level was obviously higher in the lesional tissues, cell models and animal models of psoriasis. After treated withRAPA, the expression and methylation levels ofTPMs were all restored in the cell models and animal models of psoriasis.RAPAinhibited cell proliferation and decreased the ratio of S phase cell in Hacat or human epidermal keratinocytes cell models of psoriasis. Finally, the activatedERK1/2 andmTORpathways in the cell model and animal model of psoriasis were suppressed by the treatment ofRAPA.RAPAcould be used as an effective agent for the treatment of psoriasis by decreasing the methylation level ofTPM1 andTPM2 via inhibiting theERK1/2 andmTORsignalling pathways.
Clinical outcomes and patient satisfaction following total hip and knee arthroplasty in patients with inherited bleeding disorders: A 20‐year single‐surgeon cohort
Authors: Anderson J. A. , Mason J. A. , Halliday B. 
Published: July 2018

IntroductionPersons with haemophilia and other inherited bleeding disorders are prone to disabling joint arthropathy frequently requiring arthroplasty for end‐stage joint disease. Higher complication rates and more modest post‐operative functional outcomes have previously been described.AimTo evaluate the clinical outcomes and patient satisfaction of persons with inherited bleeding disorders (predominantly haemophilia) undergoing total hip and knee replacement.MethodsRetrospective, single‐centre cohort study with longitudinal assessment of patients with inherited bleeding disorders who underwent total hip and knee replacement over a 20‐year period. Eligible patients were clinically assessed with Harris Hip Score (HHS), Knee Society Score (KSS), pain visual analogue scale (VAS) and a patient satisfaction questionnaire.ResultsThirty‐one patients (48 joints) met the inclusion criteria. Mean age at surgery was 49.3 years (SD: 13.1, range 21‐75 years) with a mean follow‐up of 9.33 years (1.7‐19.3). The majority (26/31) of patients had haemophilia A, predominantly severe haemophilia A (22/26). Reported pain levels were low, and patient satisfaction was high. Joint‐specific outcome scores were “good” to “excellent” in 67% of total hip replacement patients and 92% of total knee replacement patients. A low complication rate was observed, with 2 patients requiring revision surgery and 4 patients requiring re‐operation without implant revision.ConclusionArthroplasty is a reliable procedure in patients with inherited bleeding disorders with end‐stage hip or knee arthropathy. The overall complexity of this group is highlighted, and the need for multidisciplinary care is emphasised.
Developing a generic tool to routinely measure the impact of health libraries
Authors: Ayre Stephen , Brettle Alison , Gilroy Dominic , Knock Douglas , Mitchelmore Rebecca , Pattison Sophie , Smith Susan , Turner Jenny 
Published: July 2018

AbstractBackgroundHealth libraries contribute to many activities of a health care organisation. Impact assessment needs to capture that range of contributions.ObjectivesTo develop and pilot a generic impact questionnaire that: (1) could be used routinely across all English NHS libraries; (2) built on previous impact surveys; and (3) was reliable and robust.MethodsThis collaborative project involved: (1) literature search; (2) analysis of current best practice and baseline survey of use of current tools and requirements; (3) drafting and piloting the questionnaire; and (4) analysis of the results, revision and plans for roll out.FindingsThe framework selected was the International Standard Methods And Procedures For Assessing The Impact Of Libraries (ISO 16439). The baseline survey (n = 136 library managers) showed that existing tools were not used, and impact assessment was variable. The generic questionnaire developed used a Critical Incident Technique. Analysis of the findings (n = 214 health staff and students), plus comparisons with previous impact studies indicated that the questionnaire should capture the impact for all types of health libraries.ConclusionsThe collaborative project successfully piloted a generic impact questionnaire that, subject to further validation, should apply to many types of health library and information services.
Development and validation of a liquid chromatography/tandem mass spectrometry method for the simultaneous quantification of serotonin and thromboxane B2 from activated platelets
Authors: Minet Valentine , Evrard Jonathan , Vancraeynest Christelle , Dogné Jean‐Michel , Mullier François , Pochet Lionel 
Published: July 2018

AbstractIntroductionAvailability of a rapid and reliable platelet activation assay avoiding limitations of current techniques would be valuable to diagnose heparin‐induced thrombocytopenia and platelet secretion disorders.ObjectivesThe first aim was to develop and validate an ultra‐performance liquid chromatography‐tandem mass spectrometry (UHPLC‐MS/MS) method to quantify in a single run TxB2 synthesized and serotonin released from platelets. The second aim was to use our method in association with light transmission aggregometry (LTA) to select good platelet responders for the diagnosis of HIT.MethodsElectrospray ionization and chromatographic separation were optimized for the simultaneous dosage of serotonin and TxB2. The method was validated according to the European Medicines Agency (EMA) guideline for bioanalytical method validation. LTA was performed with monoclonal anti‐CD9 (clone ALB6) as platelet activator to select good responders.ResultsDetection was performed using a tandem mass spectrometer with alternated positive and negative electrospray ionization. The total run time was 6 minutes. The method was validated for calibration curves, precision, accuracy, lower limit of quantification, carry‐over, selectivity, and matrix effect. Platelet response to ALB6 was highly variable among donors.ConclusionWe developed and validated a UHPLC‐MS/MS method for the simultaneous quantification of TxB2 and serotonin.
The caring behaviours of Danish pharmaconomists: insight for pharmacy technician practice around the world
Authors: Desselle Shane P , Hoh Ryan , Rossing Charlotte , Holmes Erin R , Gill Amanpreet , Zamora Lemuel 
Published: July 2018

AbstractObjectivesPharmacy workforce support personnel are being accorded greater scopes of practice, especially Danish pharmaconomists [pharmacy technician, experts in pharmaceuticals with a 3-year degree]. The aims of this study were to assess pharmaconomists’ caring behaviours and identify factors related to those behaviours.MethodsA self-administered survey was distributed to a random sample of pharmaconomists in Denmark. The survey assessed caring behaviours using the Caring Behaviours Assessment and acquired data to ascertain their employers’ culture, respondent’s need for achievement, personality traits, commitment and work-related characteristics. Descriptive statistics provided insight into pharmacomomists’ predispositions, and bivariate analyses were used to identify associations of pharmaconomists’ caring with other variables under study.Key findingsOver 300 pharmaconomists responded (52.2% response rate). Pharmaconomists reported generally high levels (well above the median on the 5-point scale) of caring behaviours. They reported higher levels (upper range of 5-point scale) of empathic behaviours, such as treating patients with respect and seeing things from the patient’s point of view but somewhat lower levels of encouraging the patient to talk about how they feel and praising the patient’s efforts, which could otherwise help patients cope with and improve their self-efficacy for disease management. Pharmaconomist caring was associated with practice setting, organisational culture and commitment to their employer.ConclusionsPharmaconomists reported performing behaviours that were empathic, but less frequently those associated with facilitating greater patient autonomy. Caring behaviours were associated with a number of variables related to practice setting. The findings can help to inform educational pedagogy and pharmacy personnel management.
Amyloid precursor protein is overexpressed in bladder cancer and contributes to the malignant bladder cancer cell behaviors
Authors: Zhang Dongqing , Zhou Changkuo , Li Yan , Gao Lijian , Pang Zhipeng , Yin Gang , Shi Benkang 
Published: July 2018

ObjectivesTo investigate the expression of amyloid precursor protein in bladder cancer, and to study its role in malignant bladder cancer cell behaviors.MethodsImmunohistochemistry and western blotting analysis were used to detect the amyloid precursor protein level in bladder cancer tissues and cell lines. The effect of amyloid precursor protein on bladder cancer cell proliferation, migration, invasion and cell cycle was evaluated by using small interfering ribonucleic acid. The levels of RAS, RAF, MEK, phosphorylated MEK, extracellular regulated protein kinases, phosphorylated extracellular regulated protein kinases, protein kinase B and phosphorylated protein kinase B were determined by western blot after amyloid precursor protein knockdown. The effect of amyloid precursor protein on the extracellular regulated protein kinases pathway was further evaluated using extracellular regulated protein kinases pathway agonist, ceramide C6.ResultsThe expression of amyloid precursor protein was significantly increased in the human bladder cancer tissues compared with matched normal bladder tissues. The overexpression of amyloid precursor protein was significantly associated with tumor stage, tumor size, histological grade and lymph node metastasis. The proliferation, migration and invasion of human bladder cancer cells were significantly inhibited by the silencing of amyloid precursor protein. In addition, knockdown of amyloid precursor protein arrested the cell cycle progression of bladder cancer cells in the G2/M phase. Mechanistic analysis showed that knockdown of amyloid precursor protein significantly decreased the phosphorylation of extracellular regulated protein kinases. Ceramide C6 could rescue the malignant bladder cancer cell behaviors inhibited by the silencing of amyloid precursor protein.ConclusionsAmyloid precursor protein is upregulated in bladder cancer, and might be closely associated with bladder cancer cell growth and survival. Amyloid precursor protein could potentially be used as a therapeutic target for bladder cancer treatment.
Mesurer le travail des enfants: qui faut‐il interroger?
Authors: JANZEN Sarah A. 
Published: July 2018

RésuméLa mesure du travail des enfants est une entreprise difficile et incertaine, mais cruciale puisque le ciblage de l'aide en dépend. Dans ce contexte, l'auteure montre à partir de données tanzaniennes, et en utilisant deux définitions du travail des enfants, que le choix du répondant influe considérablement sur les chiffres obtenus, supérieurs de 35 à 65 pour cent quand ce sont les déclarations des enfants qui sont prises en compte plutôt que celles des adultes. Le biais touche 14 à 31 pour cent de l'échantillon. L'écart diminue avec l'âge de l'enfant et se creuse lorsque le répondant adulte est hostile au travail des enfants.
Les transferts de fonds et leur effet sur les indicateurs du marché du travail: illustration par le cas mexicain
Authors: SHARMA Amarendra , CÁRDENAS Oscar 
Published: July 2018

RésuméL'effet des envois de fonds sur les indicateurs du marché du travail est abondamment traité dans la littérature, à partir de microdonnées en général. Les macrodonnées, plus rarement utilisées, posent un problème d'endogénéité lié aux corrélations possibles de la variable relative aux transferts. Dans cette analyse, fondée sur des données de panel relatives au Mexique, les auteurs cherchent à contourner le problème en utilisant un estimateur GMM en système. Ils montrent que les envois de fonds élèvent les taux d'activité tout en réduisant le nombre des heures travaillées, la fréquence de l'emploi «critique» (c'est‐à‐dire de mauvaise qualité) et la durée des épisodes de chômage.
L'inspection du travail, un outil efficace quand les règles et sanctions varient selon les effectifs de l'entreprise? Analyse à partir du cas péruvien
Authors: VIOLLAZ Mariana 
Published: July 2018

RésuméL'auteure analyse l'effet de la rigueur du dispositif de mise en application de la législation sur le respect effectif des droits au travail lorsque les règles et sanctions dépendent des effectifs de l'entreprise. À partir de données de l'enquête auprès des ménages du Pérou (2008–2013), elle examine l'influence en la matière de facteurs déjà connus (effet dissuasif de l'inspection, glissement vers le secteur informel) mais aussi celui du «redimensionnement», stratégie utilisée par les employeurs pour se prévaloir de régimes plus favorables. Elle montre que l'intensité du dispositif de contrôle n'influe que modérément sur le degré de conformité. L'effet «redimensionnement» est lui aussi limité.
Nonisothermal crystallization kinetics and stability of leucite and kalsilite from K2O‐Al2O3‐SiO2 glasses
Authors: Christopoulou Georgia , Modarresifar Farid , Allsopp Benjamin L. , Jones Alan H. , Bingham Paul A. 
Published: July 2018

AbstractThe crystallization mechanisms and elemental stability of leucite and kalsilite formed from K2O‐Al2O3‐SiO2 glasses were investigated by X‐ray powder diffraction (XRD), X‐ray fluorescence (XRF), Raman spectroscopy and differential scanning calorimetry (DSC). Glass samples with compositions along the leucite‐kalsilite tie‐line were produced by melt processing and were then heat‐treated at 850, 950, and 1250°C for times ranging from 5 minutes to 1000 hours. Kalsilite is an unstable phase that behaves as an intermediate precursor to leucite. Crystalline materials in which kalsilite is the major phase lose potassium upon prolonged heat treatment (1000 hours at 1250°C), in contrast to those with leucite, in which little or no compositional alteration is detected. The formation of leucite from stoichiometric kalsilite is accompanied by the formation of potassium‐doped alumina. The activation energies for leucite and kalsilite crystallization, determined via application of the Kissinger equation to thermal analysis data, were 579 and 548 kJ/mol, respectively. Finally, production of pure leucite can be achieved with more favorable crystallization kinetics when starting with off‐stoichiometric compositions.
Doping manganese into CsPb(Cl/Br)3 quantum dots glasses: Dual‐color emission and super thermal stability
Authors: He Meiling , Cheng Yinzi , Shen Linli , Zhang Huiling , Shen Chenyang , Xiang Weidong , Liang Xiaojuan 
Published: July 2018

AbstractCsPbX3 (X = Cl, Br, I) perovskite quantum dots (QDs) represent bright and tunable photoluminescence, it is regrettable that the air instability and poor water resistant properties prevent their application in optoelectronic devices. At the same time, the toxicity of lead is also a major factor restricting its development. As a consequence, we demonstrate the partial replacement of Pb with Mn through conventional melt‐quenching and heat‐treatment method preparation of Mn‐doped CsPb(Cl/Br)3 QD glass. Mn‐doped CsPb(Cl/Br)3 QD glass exhibits high luminescent intensity like QDs. It is important that Mn‐doped CsPb(Cl/Br)3 QD glass with Dual‐Color maintained the same lattice structure like Mn‐doped CsPb(Cl/Br)3 QDs, and highly homogeneous spectral characteristics of Mn luminescence. The intensity and position of this Mn‐related emission are also tunable by altering the experimental parameters, such as the Pb‐to‐Mn feed ratio, annealing temperature. More importantly, the as‐prepared orange Mn‐doped CsPb(Cl/Br)3 QD glass was employed to fabricate white LEDs combined with a commercial Ce3+:Y3Al5O12 phosphor‐in‐glass (Ce‐PiG) on top of a InGaN blue chip. And the constructed WLEDs generate a warm white with an optimal luminous efficacy (LE) of 67.00 lm/W, a high CRI of 81.4, and a low CCT of 4902 K.
Association of mood disorders with cardiovascular disease risk factors in overweight and obese youth with elevated blood pressure
Authors: Medrano Leah , Amatya Kaushalendra , Vizthum Diane , Fadrowski Jeffrey J. , Brady Tammy M. 
Published: July 2018

The American Heart Association defines mood disorders (MDO) as a tier‐II cardiovascular disease risk factor in children. Cross‐sectional analysis of overweight/obese children referred to an obesity hypertension clinic revealed 37% had a MDO (defined by clinical diagnosis or Patient Health Questionnaire‐9/‐A score ≥10), 55% had confirmed hypertension, and 75% left ventricular hypertrophy (LVH). Children with MDOs were older, had greater measures of adiposity, and had a greater prevalence of hypertension (78%) than those without MDOs (42%; P = .04). Hypertensive children were 2.8 times more likely to have a MDO than those without (52% vs 18%; P = .02). Multivariable logistic regression revealed a statistically significant independent association of MDOs with hypertension (Odds Ratio [OR] 6.3, P = .048), but not LVH (LVMI ≥ 51 g/m2.7; OR 1.13, P = .88). Overall, the prevalence of MDOs in this group of overweight/obese children with elevated blood pressure was well above national averages, suggesting that at‐risk youth, particularly those with confirmed hypertension, should be regularly screened for MDOs.
Photobiomodulation and different macrophages phenotypes during muscle tissue repair
Authors: Souza Nadhia H. C. , Mesquita‐Ferrari Raquel A. , Rodrigues Maria Fernanda S. D. , da Silva Daniela F. T. , Ribeiro Beatriz G. , Alves Agnelo N. , Garcia Mónica P. , Nunes Fábio D. , da Silva Junior Evaldo M. , França Cristiane M. , Bussadori Sandra K. , Fernandes Kristianne P. S. 
Published: July 2018

AbstractMacrophages play a very important role in the conduction of several regenerative processes mainly due to their plasticity and multiple functions. In the muscle repair process, while M1 macrophages regulate the inflammatory and proliferative phases, M2 (anti‐inflammatory) macrophages direct the differentiation and remodelling phases, leading to tissue regeneration. The aim of this study was to evaluate the effect of red and near infrared (NIR) photobiomodulation (PBM) on macrophage phenotypes and correlate these findings with the repair process following acute muscle injury. Wistar rats were divided into 4 groups: control; muscle injury; muscle injury + red PBM; and muscle injury + NIR PBM. After 2, 4 and 7 days, the tibialis anterior muscle was processed for analysis. Macrophages phenotypic profile was evaluated by immunohistochemistry and correlated with the different stages of the skeletal muscle repair by the qualitative and quantitative morphological analysis as well as by the evaluation of IL‐6, TNF‐α and TGF‐β mRNA expression. Photobiomodulation at both wavelengths was able to decrease the number of CD68+ (M1) macrophages 2 days after muscle injury and increase the number of CD163+ (M2) macrophages 7 days after injury. However, only NIR treatment was able to increase the number of CD206+ M2 macrophages (Day 2) and TGF‐β mRNA expression (Day 2, 4 and 7), favouring the repair process more expressivelly. Treatment with PBM was able to modulate the inflammation phase, optimize the transition from the inflammatory to the regeneration phase (mainly with NIR light) and improve the final step of regeneration, enhancing tissue repair.
Clinical associations between acetylcholine levels and cholinesterase activity in saliva and gingival crevicular fluid and periodontal diseases
Authors: Apatzidou Danae A. , Iskas Achilleas , Konstantinidis Antonis , Alghamdi Abeer M. , Tumelty Maria , Lappin David F. , Nile Christopher J. 
Published: July 2018

AbstractAimThe oral mucosa possesses a non‐neuronal cholinergic system. This study aimed to determine clinical evidence for a role of cholinergic mechanisms in the pathogenesis of periodontal diseases.Materials and MethodsFifty healthy participants, 52 patients with gingivitis and 49 with periodontitis were recruited. Full periodontal parameters were recorded and saliva and gingival crevicular fluid (GCF) collected. Levels of acetylcholine and inflammatory mediators were quantified using commercially available assay kits. Acetylcholinesterase and butyrylcholinesterase activities were measured using a published biochemical assay.ResultsAcetylcholine levels are significantly elevated in saliva and GCF, whereas GCF levels of butyrylcholinesterase activity are significantly decreased, in patients with periodontal diseases. Acetylcholine levels in saliva and GCF correlated positively with clinical markers of disease severity and with increased levels of IL‐17A and IL‐17F. In contrast, butyrylcholinesterase activity levels in GCF showed significant negative correlations with clinical markers of disease severity and IL‐17A and IL‐17F levels. None of the findings were due to smoking.ConclusionsElevated acetylcholine levels and reduced butyrylcholinesterase activity are clinically associated with periodontal diseases and elevated levels of IL‐17A and IL‐17F. Therefore, non‐neuronal cholinergic mechanisms may influence IL‐17 biology and the aetiopathogenesis of periodontal diseases and therefore are possible therapeutic targets.