bluuebunny/crossref_metadata_2025_split huggingface dataset:
Stability-Indicating Liquid Chromatographic Method Development for the Simultaneous Determination of Amitriptyline Hydrochloride and Propranolol Hydrochloride in Tablet Dosage Form
Authors: Chabala Obi Reddy , Haque Md Simon , Thirumoorthy Durai Ananda Kumar 
Published: December 2024

Abstract
The combination of the tricyclic antidepressant amitriptyline hydrochloride (AMH) and the non-selective beta-adrenergic blocker propranolol hydrochloride (PPH) is used for migraine prophylaxis. Higher doses of AMH trigger cardiac arrhythmias, anxiety, tachycardia, convulsions, hyperglycemia and anticholinergic side effects. The combined dosage formulation of AMH and PPH leads to drug–drug interactions; causes sedation, xerostomia, dysuria, insomnia and bradycardia; and results in patient non-compliance. The quantification of AMH and PPN becomes essential, especially for combination formulations, in addition to regular quality control to avoid clinical issues. Considering these facts into account, the reverse-phase -high-performance liquid chromatography (RP-HPLC) method was developed in accordance with International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human Use Q2(R1) guidelines for the simultaneous determination of AMH and PPH. The HPLC separation was performed on an HPLC system (Shimadzu, Japan, Prominence I series 2030C) using a Shimadzu Shim-Pack GIST C18 column (100 mm × 4.6 mm, 5 μ), which was equipped with an ultraviolet detector at the isosbestic point 238 nm. The mixture of acetonitrile and orthophosphoric acid (pH 3.5) in a ratio of 35:65 v/v with a flow rate of 0.75 mL/min was used as the mobile phase. The regression coefficients of AMH (r2 &gt; 0.998) and PPH (r2 &gt; 0.999) show good linearity between peak areas and drug concentration ranges. The limits of detection (AMH = 0.67 μg/mL, PPH = 0.67 μg/mL) and limits of quantification (AMH = 2.04 μg/mL, PPH = 2.05 μg/mL) demonstrated the higher detection sensitivity of the proposed method.
Imaging using diffusion-weighted whole-body imaging with background signal suppression for methotrexate-associated lymphoproliferative disorder: A case report
Authors: Mochizuki Takeshi , Otani Naoko , Ando Mari , Hiroshima Ryo , Yano Koichiro , Ikari Katsunori , Okazaki Ken 
Published: December 2024

ABSTRACT
Patients with rheumatoid arthritis (RA) receiving immunosuppressive therapy including methotrexate (MTX) are at risk of developing lymphoproliferative disorder (LPD). Herein, we report the case of a 61-year-old man who has been treated with MTX and sulfasalazine for seropositive RA since the age of 52 years. He underwent diffusion-weighted whole-body imaging with background signal suppression (DWIBS), which revealed high-intensity lesions in the affected lymph nodes of the cervical, clavicular, and axillary regions. Follow-up DWIBS after MTX withdrawal showed the suppression or disappearance of the high-intensity lesions. This case demonstrates the potential of DWIBS as a new standard imaging modality for MTX-LPD in patients with RA in clinical practice.
Foot‐and‐mouth disease virus 2B protein antagonizes STING‐induced antiviral activity by targeting YTHDF2
Authors: Xue Qiao , Ma Ke , Yang Fan , Liu Huisheng , Cao Weijun , Liu Pengfei , Zhu Zixiang , Zheng Haixue 
Published: December 2024

AbstractFoot‐and‐mouth disease virus (FMDV) infection modulates the retinoic acid‐inducible gene I (RIG‐I)‐like receptors (RLRs) pathways to inhibit the innate immune responses in the host. However, the mechanism by which FMDV antagonizes the DNA‐induced signaling pathway remains to be clarified. In this study, we determined that FMDV infection inhibited stimulator of interferon genes (STING) at the levels of both mRNA and protein expression, and FMDV 2B and 3Cpro proteins promoted STING decline. FMDV 3Cpro induced the decrease in STING depending on its protease activity. FMDV 2B reduced STING expression by disrupting its mRNA level. Mechanistically, 2B inhibited the mRNA of STING by recruiting YTH m6A RNA‐binding protein 2 (YTHDF2) to bind to STING mRNA, repressing the generation of FMDV‐induced type‐I interferon and facilitating virus replication. This effect was triggered by residue 105 of 2B. The 2B K105A mutant FMDV was successfully rescued, and further studies showed that the pathogenicity was attenuated by mutation at site K105 of FMDV 2B. YTHDF2 also promoted FMDV replication through interferon‐dependent and interferon‐independent pathways. Moreover, YTHDF2‐deficient mice showed stronger resistance to FMDV infection. Our study reveals a potential mechanism for FMDV 2B negatively modulating innate immunity at transcriptional levels, promoting the understanding of immune evasion and YTHDF2 function in the FMDV infection process.
Acute-on-Chronic Inflammation and Patients’ Risk for Renal Support in Critically Ill Patients
Authors: Graham Julie-Kathryn , Quillin-Mcewan Molly , Kelley Christina 
Published: December 2024

Hypertension (HTN) and heart failure (HF) can chronically activate the renin-angiotensin-aldosterone system, a mechanism designed to maintain hemodynamic stability by reabsorption of water and electrolytes. Additionally, this system activates the sympathetic nervous system to increase vagal tone. When these patients face acute illness requiring hospitalization, the acute stressor or pathogen also activates the sympathetic nervous system. The combination of activation of both systems puts patients at increased risk of organ failure, specifically renal failure. With early recognition of renal insult, organ damage can be reversed. C-reactive protein (CRP) and D-dimer are commonly used to measure acute inflammation. These biomarkers can alert critical care nurses to excessive inflammation in patients with underlying HTN and HF, enabling nurses to make informed decisions to intervene at the earliest sign of renal failure. This retrospective study of adult SARS-CoV-2 patients in an intensive care unit setting sought to examine the relationship of CRP, D-dimer, and the need for eventual renal support in patients with HF and HTN. Of the sample (n + 189), mean age was 62 (SD = 14.0), and most (70.9%) were male. Thirty-nine patients (20.6%) required renal support. Of the cases requiring renal support, 21 (53.8%) had a history of prior renal disease (P < 0.001, r = 0.351). History of HTN was significantly correlated with requirement for renal support (P = 0.010, r = 0.187). D-dimer (P = 0.038, η = 1.0) and CRP (P = 0.018, η = 0.924) were also significant. Survival was significantly worse in the renal support group (P < 0.001, r = −0.310). D-dimer and CRP were correlated with more severe illness and need for renal support. Study findings have implications for future validation research of chronic inflammation and risk for renal support during acute severe illness.
Adult Pancreatoblastoma Presenting with Obstructive Jaundice: A Case Report and Literature Review
Authors: Leong Yeu Chyi , Liu Hung-Wei , Chen Yaw-Sen 
Published: December 2024

Abstract
Pancreatoblastoma (PB), a rare malignancy originating from pancreatic exocrine cells, predominantly affects pediatric patients but is exceptionally rare in adults. We present a case of adult PB presenting with obstructive jaundice secondary to a pancreatic head mass. Despite nonspecific clinical and radiological manifestations, pathological evaluations confirmed the diagnosis. Treatment involved Roux-en-Y cholecytojejunostomy biliary bypass followed by systemic chemotherapy and local radiotherapy due to advanced disease stage with liver metastases. This case highlights the diagnostic challenges and aggressive nature of PB in adults, necessitating interdisciplinary collaboration for optimal management.
Gender-affirming Chest Surgery: A Prospective Series of 155 Consecutive Cases of Double Incision Mastectomy
Authors: Vico Pierre G. , Salmon Salomé 
Published: December 2024

Background:
Mastectomy is the first single surgery that transgender men and nonbinary people frequently undergo. We present a prospective series of 155 cases of double incision mastectomy (DIM) with nipple-areolar complex (NAC) graft.

Methods:
From January 2019 to December 2022, 165 outpatients were operated on, and 155 are included in the study. We analyzed age, resection weight, blood loss, duration of the procedure, complications, and follow-up. The Student t test combined with the nonparametric Mann-Whitney U test was used for statistical analysis. If the P value was less than 0.05, the test was considered significant.

Results:
A total of 111 (72.6%) patients experienced no complications. Complications (27.4%) were minor: 4 seromas, 8 hematomas, 10 partial necrosis of the NAC, and 8 depigmentation of the NAC. Thirteen patients presented axillary dog ears requiring secondary excision. Two patients had hypertrophic scars. No case of infection was observed. We find a statistically significant correlation between hematoma and perioperative blood loss (P < 0.05), and between dog ears requiring secondary procedure and body mass index (P < 0.001), weight of resection (P < 0.01), and blood loss (P < 0.001).

Conclusions:
DIM with NAC graft is a safe, reliable, top surgery procedure. Complications are minor, and postoperative morbidity is low. This study shows a correlation between blood loss during surgery and postoperative hematoma, and between dog ears requiring a surgery and body mass index, weight of resection, and blood loss. This surgery gives nice aesthetic results enhancing patients’ quality of life by improving body image.
Staff and work environment factors associated with workplace violence in hospitals: Comparison of units with high versus low rates of violence
Authors: Derscheid Della J. , Arnetz Judith E. 
Published: December 2024

Abstract

Objective
This study examined healthcare worker perceptions of physical environment and safety climate contributors to workplace violence.

Methods
Inpatient hospital employee (2015) convenience sample survey (N = 327) comparisons were via Mann Whitney, Wilcoxon Rank Sum, or Chi-square to determine response differences by workplace violence experience (yes/no), and hospital units (higher/lower) violence rates.

Results
Employees with workplace violence experience and units with higher violence rates identified a higher number of contributing physical environment factors. Safety climate ratings were higher among employees without workplace violence experience but did not differ between hospital units with higher versus lower violence rates.

Conclusions
Prioritization of staff perceived climate safety and physical environment contributors can support organizational efforts to mitigate specific workplace violence hazards on hospital units.
Patterns of Mental Health and Resilience among Nurses and Physicians throughout the COVID-19 Pandemic: A Three-Year Longitudinal Study
Authors: Vala Katelyn C. , Fishbein Joanna S. , Bellehsen Mayer H. , Parashar Nidhi , Yacht Andrew C. , Young John Q. , Schwartz Rebecca M. 
Published: December 2024

Abstract

Objective
The COVID-19 pandemic is associated with increased difficulties in emotional wellbeing among healthcare workers. The current investigation assesses how changes in depression and anxiety symptoms fluctuate relative to resilience across three time points: baseline, one-year, and two-year follow up among a cohort of physicians and nurses (N = 728).

Methods
Generalized linear mixed modeling for repeated measures was used to assess the association between resilience and mental health symptoms using validated measures for all variables.

Results
The odds of probable anxiety were reduced by 81% for each one-unit increase in resilience (OR = 0.19, 95% CI [0.14, 0.26]). The odds of probable depression were reduced by 88% for each one-unit increase in BRS (OR = 0.12, 95% CI [0.07, 0.2]).

Conclusions
Results highlight the importance of developing and implementing interventions that foster resilience among healthcare workers.
Mental Health Support for First Responders Provided Outside of California’s Workers’ Compensation System
Authors: Quigley Denise D. , Qureshi Nabeel , Dworsky Michael , Meredith Lisa S. 
Published: December 2024

Abstract

Objective
Concern about psychiatric injuries for first responders has grown. We examine alternatives to workers’ compensation (WC).

Method
We conducted 15 interviews across 5 California departments contracted with non-WC mental health (MH) providers. We performed content analysis.

Results
MH support outside of WC most often was a dedicated contract with outside MH providers for assessment, diagnosis, and treatment. Departments had formal financing mechanisms with union and city support designating funding for MH care. Frustration and past WC experiences drove the desire for WC alternatives. First responders reported high levels of stigma associated with MH care. Compared to WC, these alternatives allowed more comprehensive services to injured workers along with ease of accessing care, expediting the healing process.

Conclusion
Contracting culturally competent MH providers outside of WC for first responders has potential and needs formal evaluation.
Cost-effectiveness of a Low-cost Educational Messaging and Prescription-fill Reminder Intervention to Improve Medication Adherence Among Individuals With Intellectual and Developmental Disability and Hypertension
Authors: Chen Brian , McDermott Suzanne , Salzberg Deborah , Zhang Wanfang , Hardin James W. 
Published: December 2024

Background:
Adults with intellectual and developmental disabilities (IDDs) have a similar prevalence of hypertension as the general population, but a higher rate of medication nonadherence at 50% compared with the average of 30%.

Objectives:
To assess the cost-effectiveness of educational messaging and prescription-fill reminders to adults with IDD and hypertension and their helpers among Medicaid members in a randomized control trial.

Research Design:
The authors calculated net cost savings by subtracting per-participant intervention costs from differences in spending between preintervention/postintervention cases versus controls. Using bootstrap samples, they assessed the probability of positive cost savings. They used quantile and logistic regression to examine which members contributed to the cost savings and to identify future high-cost members at baseline.

Subjects:
Four hundred twelve members with IDD and their helpers were recruited from the South Carolina Medicaid agency in 2018.

Measures:
Intervention costs were determined using labor and communication costs. Health expenditures were obtained from South Carolina’s all-payer claims database, using actual Medicaid expenditures and total all-payer expenditures estimated with cost-to-charge ratios.

Results:
The intervention, which cost $26.10 per member, saved $1008.02 in all-payer spending and $1126.42 in Medicaid payments per member, respectively, with 78% and 91% confidence. Cost savings occurred among members above the 85th percentile of spending, and those using the emergency department or inpatient services at least twice at baseline were predicted to be future high-cost members.

Conclusions:
The intervention is cost-saving, and insurers can prospectively identify and target members who will likely benefit.
Safety observations in neuroblastoma patients undergoing 18F-mFBG PET
Authors: Pandit-Taskar Neeta , Basu Ellen , Balquin Eloisa , Mozley P. David , Jacobson Arnold F. , Modak Shakeel 
Published: December 2024

Objective
Limited safety data have been published on fluorine-18 (18F) meta-fluorobenzylguanidine (mFBG), a new PET radiopharmaceutical for imaging neural crest and neuroendocrine tumors. As part of a prospective clinical trial, safety data in patients with neuroblastoma were collected and analyzed.

Methods
Between April 2015 and January 2022, 27 patients with neuroblastoma underwent 18F-mFBG PET imaging as part of an ongoing single-center phase 1/2 trial (NCT 02348749). Pre- and postinjection safety assessments were performed, including vital sign measurement and observation for occurrence of adverse events (AEs).

Results

mFBG administration resulted in no significant changes in measured vital signs. Two subjects had transient, grade 1 facial flushing shortly after the administration, which resolved within a few minutes. Neither subject had a clinically significant change in pulse or blood pressure on postadministration measurements.

Conclusion
In this investigation of the potential clinical utility of mFBG PET imaging, no significant adverse safety signals were noted. Two mild, self-limited AEs were observed, without associated changes in vital signs. No grade 2 or higher AEs were noted. The findings are consistent with a favorable safety profile for mFBG in the target population of patients with neuroblastoma.
The evolution and hotspots of radioactive iodine therapy in hyperthyroidism: a bibliometric analysis
Authors: Chen Shi-qi , Gou Rui-qin , Zhang Qing 
Published: December 2024

Objective
Hyperthyroidism, a prevalent endocrine disorder, disrupts metabolic balance and cardiovascular health, affecting millions globally. Radioactive iodine (RAI), a treatment for hyperthyroidism, employs high-energy beta particles to reduce thyroid tissue, lowering volume and hormone levels. This study utilizes bibliometric analysis to outline RAI’s evolution and identify hotspots in hyperthyroidism treatment.

Methods
A total of 2904 articles and reviews published between 1981 and 2023 were retrieved from the Web of Science Core Collection using the research strategy. Bibliometric analyses, employing VOSviewer and CiteSpace, were performed to visualize the cooperation network, evolution, and hot topics.

Results
Annual publications rose in waves, 2904 papers from 2564 institutions, and 84 countries. The USA led, contributing the largest share, with the journal Thyroid dominating publication. The University of Pisa contributed the most articles. Co-occurrence analysis classified keywords into five clusters: treatment mechanism, safety, effectiveness assessment, individualized radioactive dosage, and management. The development of RAI therapy for hyperthyroidism can be divided into three stages: safety and efficacy assessment, personalized treatment plans, and treatment of drug-resistant and surgery-resistant hyperthyroidism.

Conclusion
Attention to RAI in hyperthyroidism should be significantly increased. It is necessary to establish collaborations between authors, countries, and institutions to promote the development of this field. Recent research has focused on personalized radioactive dosage formulation and follow-up. Future studies are likely to concentrate on drug-resistant and surgery-resistant hyperthyroidism, which is also worthy of investigation. These findings provide a new perspective on the study of RAI in hyperthyroidism, potentially contributing to the improvement of the quality of life for patients with hyperthyroidism.
The potential of circulating nonesterified fatty acids and sphingolipids in the biological understanding of cognitive decline and dementia
Authors: Moseholm Kristine F. , Meineche Josefine T. , Jensen Majken K. 
Published: December 2024

Purpose of review
Cognitive decline and late-onset dementia pose significant challenges in aging societies, and many dementia cases could be prevented or delayed through modification of associated risk factors, many of which are tied to cardiovascular and metabolic dysfunction. As individuals age, the blood-brain barrier becomes more permeable, easing the exchange of molecules between the bloodstream and the brain. Consequently, blood-based biological markers (so-called biomarkers) provide a minimally invasive and accessible means of accessing molecular changes associated with aging and neurodegeneration.

Recent findings
Circulating free fatty acids, also called nonesterified fatty acids (NEFAs), and sphingolipids are associated with cardiovascular disease, insulin resistance, and diabetes; thus, could be promising candidates as biomarkers for cognitive decline and dementia.

Summary
The opportunity to study such minimally invasive biomarkers further opens up potential new avenues for improved understanding of the underlying biology of diseases of the brain
When a Horseshoe Is Not a Lucky Find
Authors: Yim Wesley , Subramaniam Sathyaseelan 
Published: December 2024

Abstract
Identification of a neonatal horseshoe kidney in the emergency department is uncommon but has implications for future healthcare planning for patients. We present a case of a neonate with bloody diapers who was evaluated with renal point-of-care ultrasound (POCUS) that identified a horseshoe kidney. The ultrasound was remarkable for its clear visualization of the isthmus of the horseshoe kidney in the midline abdomen. Midline interrogation is not part of the routine technique for ultrasound imaging of the kidney but should be considered when the limits of the lower pole of bilateral kidneys are difficult to visualize. This case highlights the importance of understanding normal anatomical landmarks when performing renal POCUS to identify renal anomalies.
“Room of Horrors”
Authors: Capua Tali , Arnon Maya , Rozenberg Moriya , Perets Efrat , Samuel Nir , Levy Dana Adatto , Elmaliach Nadav , Padova Hagit , Rimon Ayelet 
Published: December 2024

Objective:
This pilot study aimed to assess the effectiveness of a “room of horrors” (RoH) simulation in identifying patient safety threats in a pediatric emergency department (PED) and to evaluate health care workers’ (HCWs’) perceptions of the experience.

Methods:
We developed an RoH simulation featuring 25 potential safety hazards derived from actual PED incidents and “never events.” The teams of physicians and nurses who participated in the simulation identified as many hazards as they could within a 10-minute window followed by a debriefing session during which errors were corrected and missed hazards were pointed out. The participants’ perceptions were measured on a 5-point Likert scale of a questionnaire.

Results:
Twenty sessions that included a total of 45 participants were conducted. The teams identified an average of 23 of 25 safety threats, yielding a mean detection rate of 86% for electronic medical record errors, 95% for physical space errors, and 97% for communication errors. The simulation received high ratings for overall positive experience (4.88/5) and impact on daily activities (4.68/5).

Conclusion:
The RoH simulation experience was effective in evaluating the level of retention of earlier training and the ability of multidisciplinary teams to correctly identify major safety threats in a PED setting. The participants found the experience valuable and engaging. Future research should focus on other means by which the retention of learned skills can be reinforced and new safety threats can be identified with a high level of alertness.
Malignant Phyllodes Tumor With Sphenoid Bone Metastasis Detected on 99mTc-MDP SPECT/CT and 18F-FDG PET/CT
Authors: Wakankar Ritwik , Dharmashaktu Yamini , Venugopal Akhil , Kumar Rakesh 
Published: December 2024

Abstract
Phyllodes tumors (PTs) are rare fibroepithelial tumors accounting for less than 1% of all breast neoplasms. They are malignant in 20% of cases. Only a few cases of malignant PT metastatic to bone have been reported. We report a case of metastatic malignant PT of the breast involving the greater wing of the sphenoid bone and discuss the radiological findings of 99mTc-methylene diphosphonate skeletal scintigraphy, SPECT/CT, and 18F-FDG PET/CT. To our knowledge, this is the first time such a case has been reported in literature.