bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lunar Tide in the F Region Ionosphere
Authors: Forbes Jeffrey M. , Zhang Xiaoli 
Published: August 2019

AbstractIn this paper the magnetic latitude (±50°) and longitude, local solar time (LST), and month‐to‐month variability of the M2 lunar semidiurnal tide in the ionosphere is revealed through analysis of topside F region electron density measurements by the Planar Langmuir Probe instrument on the CHAMP satellite during the two 5‐year periods 2001–2005 and 2005–2009. The local time precession rate of CHAMP is such that 5 years is required to obtain full LST coverage for every month of the year at each latitude of interest. Thus, each 5‐year period is compressed into one equivalent year. M2 amplitudes, expressed in terms of percent residuals from a background value, range between about 3% and 20% and vary considerably with LST and magnetic longitude. Longitude variations in principle arise from zonal asymmetries in lunar tidal forcing due to Earth and ocean tides, as well as magnitude of Earth's magnetic field. The O1 diurnal tide is also discussed in the context of CHAMP and other measurements of the F region ionosphere.
Nighttime Magnetic Perturbation Events Observed in Arctic Canada: 1. Survey and Statistical Analysis
Authors: Engebretson M. J. , Pilipenko V. A. , Ahmed L. Y. , Posch J. L. , Steinmetz E. S. , Moldwin M. B. , Connors M. G. , Weygand J. M. , Mann I. R. , Boteler D. H. , Russell C. T. , Vorobev A. V. 
Published: August 2019

AbstractThe rapid changes of magnetic fields associated with large, isolated magnetic perturbations with amplitudes |ΔB| of hundreds of nanotesla and 5‐ to 10‐min periods can induce bursts of geomagnetically induced currents that can harm technological systems. This paper presents statistical summaries of the characteristics of nightside magnetic perturbation events observed in Eastern Arctic Canada from 2014 through 2017 using data from stations that are part of four magnetometer arrays: MACCS, AUTUMNX, CANMOS, and CARISMA, covering a range of magnetic latitudes from 68 to 78°. Most but not all of the magnetic perturbation events were associated with substorms: roughly two thirds occurred between 5 and 30 min after onset. The association of intense nighttime magnetic perturbation events with magnetic storms was significantly reduced at latitudes above 73°, presumably above the nominal auroral oval. A superposed epoch study of 21 strong events at Cape Dorset showed that the largest |dB/dt| values appeared within an ~275‐km radius that was associated with a region of shear between upward and downward field‐aligned currents. The statistical distributions of impulse amplitudes of both |ΔB| and |dB/dt| fit well the log‐normal distribution at all stations. The |ΔB| distributions are similar over the magnetic latitude range studied, but the kurtosis and skewness of the |dB/dt| distributions show a slight increase with latitude. Knowledge of the statistical characteristics of these events has enabled us to estimate the occurrence probability of extreme impulsive disturbances using the approximation of a log‐normal distribution.
Adipose tissue contribution to plasma fibroblast growth factor 21 and fibroblast activation protein in obesity
Authors: van Baak Marleen A. , Vink Roel G. , Roumans Nadia J. T. , Cheng Christine C. , Adams Andrew C. , Mariman Edwin C. M. 
Published: August 2019

Abstract
Fibroblast growth factor 21 (FGF21) is an important regulator of energy metabolism. FGF21 is inactivated by fibroblast activation protein (FAP). We investigated whether FGF21 and/or FAP are secreted from human white adipose tissue of individuals with obesity by measuring total FGF21, active FGF21, and FAP concentrations in arterialized blood and venous blood draining the subcutaneous abdominal adipose tissue (scAT). Measurements were performed under fasting conditions and after a high fat meal before and after diet-induced weight loss in 16 adults with BMI 27–35 kg/m2. FGF21 was not released from scAT, neither before nor after weight loss in agreement with an undetectable gene expression of FGF21 in this tissue. Although scAT showed significant gene expression of FAP, no release of FAP from the tissue could be detected. The high fat meal increased postprandial circulating FGF21 but not FAP. Circulating levels of FAP but not FGF21 were significantly reduced after weight loss. On the other hand, FAP expression in scAT was increased. In conclusion, release from scAT does not appear to contribute to circulating concentrations of FGF21 and FAP and their responses to ingestion of a high fat meal or weight loss, respectively, in individuals with obesity.
Mass cytometry dissects T cell heterogeneity in the immune tumor microenvironment of common dysproteinemias at diagnosis and after first line therapies
Authors: Kourelis Taxiarchis V. , Villasboas Jose C. , Jessen Erik , Dasari Surendra , Dispenzieri Angela , Jevremovic Dragan , Kumar Shaji 
Published: August 2019

AbstractDysproteinemias progress through a series of clonal evolution events in the tumor cell along with the development of a progressively more “permissive” immune tumor microenvironment (iTME). Novel multiparametric cytometry approaches, such as cytometry by time-of-flight (CyTOF) combined with novel gating algorithms can rapidly characterize previously unknown phenotypes in the iTME of tumors and better capture its heterogeneity. Here, we used a 33-marker CyTOF panel to characterize the iTME of dysproteinemia patients (MGUS, multiple myeloma—MM, smoldering MM, and AL amyloidosis) at diagnosis and after standard of care first line therapies (triplet induction chemotherapy and autologous stem cell transplant—ASCT). We identify novel subsets, some of which are unique to the iTME and absent from matched peripheral blood samples, with potential roles in tumor immunosurveillance as well as tumor immune escape. We find that AL amyloidosis has a distinct iTME compared to other dysproteinemias with higher myeloid and “innate-like” T cell subset infiltration. We show that T cell immune senescence might be implicated in disease pathogenesis in patients with trisomies. Finally, we demonstrate that the early post-ASCT period is associated with an increase of senescent and exhausted subsets, which might have implications for the rational selection of post-ASCT therapies.
ERα-mediated alterations in circ_0023642 and miR-490-5p signaling suppress bladder cancer invasion
Authors: Wu Longxiang , Zhang Mengda , Qi Lin , Zu Xiongbing , Li Yuan , Liu Longfei , Chen Minfeng , Li Yangle , He Wei , Hu Xiheng , Mo Miao , Ou Zhenyu , Wang Long 
Published: August 2019

AbstractEpidemiological studies show obvious gender differences in the incidence and the prognosis of bladder cancer (BCa). Estrogen receptor alpha (ERα) was recently shown to play a protective role in BCa. However, the mechanisms by which ERα mediates BCa progression need to be further elucidated. In the present study, we explored the mechanisms by which ERα inhibits BCa invasion by modulating circRNA levels. ERα suppressed BCa invasion by decreasing circ_0023642 expression. Chromatin immunoprecipitation (ChIP) and luciferase assays revealed that ERα reduced circ_0023642 expression by regulating the expression of its host gene, UVRAG, at the transcriptional level. ERα decreased circ_0023642 levels and subsequently increased miR-490-5p expression, resulting in decreased EGFR expression to suppress BCa cell invasion. Circ_0023642 was demonstrated to directly bind to miR-490-5p. Notably, miR-490-5p regulated EGFR expression by binding to the miR-490-5p-binding site located in the 3′-untranslated region (UTR) of the EGFR mRNA. Preclinical studies using an in vivo mouse model also confirmed that this ERα/circ_0023642/miR-490-5p/EGFR signaling pathway suppressed BCa progression. Altogether, this newly identified pathway may serve as the basis for developing novel therapeutic strategies to treat BCa.
GREB1 induced by Wnt signaling promotes development of hepatoblastoma by suppressing TGFβ signaling
Authors: Matsumoto Shinji , Yamamichi Taku , Shinzawa Koei , Kasahara Yuuya , Nojima Satoshi , Kodama Takahiro , Obika Satoshi , Takehara Tetsuo , Morii Eiichi , Okuyama Hiroomi , Kikuchi Akira 
Published: August 2019

AbstractThe β-catenin mutation is frequently observed in hepatoblastoma (HB), but the underlying mechanism by which Wnt/β-catenin signaling induces HB tumor formation is unknown. Here we show that expression of growth regulation by estrogen in breast cancer 1 (GREB1) depends on Wnt/β-catenin signaling in HB patients. GREB1 is localized to the nucleus where it binds Smad2/3 in a competitive manner with p300 and inhibits TGFβ signaling, thereby promoting HepG2 HB cell proliferation. Forced expression of β-catenin, YAP, and c-Met induces HB-like mouse liver tumor (BYM mice), with an increase in GREB1 expression and HB markers. Depletion of GREB1 strongly suppresses marker gene expression and HB-like liver tumorigenesis, and instead enhances TGFβ signaling in BYM mice. Furthermore, antisense oligonucleotides for GREB1 suppress the formation of HepG2 cell-induced tumors and HB-like tumors in vivo. We propose that GREB1 is a target molecule of Wnt/β-catenin signaling and required for HB progression.
A next-generation tumor-targeting IL-2 preferentially promotes tumor-infiltrating CD8+ T-cell response and effective tumor control
Authors: Sun Zhichen , Ren Zhenhua , Yang Kaiting , Liu Zhida , Cao Shuaishuai , Deng Sisi , Xu Lily , Liang Yong , Guo Jingya , Bian Yingjie , Xu Hairong , Shi Jiyun , Wang Fan , Fu Yang-Xin , Peng Hua 
Published: August 2019

AbstractWhile IL-2 can potently activate both NK and T cells, its short in vivo half-life, severe toxicity, and propensity to amplify Treg cells are major barriers that prevent IL-2 from being widely used for cancer therapy. In this study, we construct a recombinant IL-2 immunocytokine comprising a tumor-targeting antibody (Ab) and a super mutant IL-2 (sumIL-2) with decreased CD25 binding and increased CD122 binding. The Ab-sumIL2 significantly enhances antitumor activity through tumor targeting and specific binding to cytotoxic T lymphocytes (CTLs). We also observe that pre-existing CTLs within the tumor are sufficient and essential for sumIL-2 therapy. This next-generation IL-2 can also overcome targeted therapy-associated resistance. In addition, preoperative sumIL-2 treatment extends survival much longer than standard adjuvant therapy. Finally, Ab-sumIL2 overcomes resistance to immune checkpoint blockade through concurrent immunotherapies. Therefore, this next-generation IL-2 reduces toxicity while increasing TILs that potentiate combined cancer therapies.
High-performance silk-based hybrid membranes employed for osmotic energy conversion
Authors: Xin Weiwen , Zhang Zhen , Huang Xiaodong , Hu Yuhao , Zhou Teng , Zhu Congcong , Kong Xiang-Yu , Jiang Lei , Wen Liping 
Published: August 2019

AbstractThe salinity gradient between seawater and river water is a clean energy source and an alternative solution for the increasing energy demands. A membrane-based reverse electrodialysis technique is a promising strategy to convert osmotic energy to electricity. To overcome the limits of traditional membranes with low efficiency and high resistance, nanofluidic is an emerging technique to promote osmotic energy harvesting. Here, we engineer a high-performance nanofluidic device with a hybrid membrane composed of a silk nanofibril membrane and an anodic aluminum oxide membrane. The silk nanofibril membrane, as a screening layer with condensed negative surface and nanochannels, dominates the ion transport; the anodic aluminum oxide membrane, as a supporting substrate, offers tunable channels and amphoteric groups. Thus, a nanofluidic membrane with asymmetric geometry and charge polarity is established, showing low resistance, high-performance energy conversion, and long-term stability. The system paves avenues for sustainable power generation, water purification, and desalination.
Catalytic amidation of natural and synthetic polyol esters with sulfonamides
Authors: Liu Hua , Zhu Yi-Ling , Li Zhi 
Published: August 2019

AbstractTriacylglycerides are naturally abundant and renewable feedstock for biofuels and chemicals. In this report, these seemingly stable compounds are shown to be reactive toward a variety of sulfonamides under Lewis acid catalysis. In these reactions, alkyl C(sp3)–O bonds are cleaved and C–N bonds constructed, providing functionalized value-added products directly from renewables. Mechanistic and scope study demonstrate that the origin of the reactivity could be the synergy of Lewis acid catalysis and neighboring group participation by the 2- or 3-acyloxy or acylamido group with respect to the reactive site. Since poly(ethylene terephthalate) (PET), a widely available consumer polyester, also contains 1,2-diol diester group as the repeating unit in the main chain, this chemistry can also be applied to efficient depolymerization of PET.
Revisiting the genetic diversity of emerging hantaviruses circulating in Europe using a pan-viral resequencing microarray
Authors: Filippone Claudia , Castel Guillaume , Murri Séverine , Ermonval Myriam , Korva Misa , Avšič-Županc Tatjana , Sironen Tarja , Vapalahati Olli , McElhinney Lorraine M. , Ulrich Rainer G. , Groschup Martin H. , Caro Valérie , Sauvage Frank , van der Werf Sylvie , Manuguerra Jean-Claude , Gessain Antoine , Marianneau Philippe , Tordo Noël 
Published: August 2019

AbstractHantaviruses are zoonotic agents transmitted from small mammals, mainly rodents, to humans, where they provoke diseases such as Hemorrhagic fever with Renal Syndrome (HFRS) and its mild form, Nephropathia Epidemica (NE), or Hantavirus Cardio-Pulmonary Syndrome (HCPS). Hantaviruses are spread worldwide and monitoring animal reservoirs is of primary importance to control the zoonotic risk. Here, we describe the development of a pan-viral resequencing microarray (PathogenID v3.0) able to explore the genetic diversity of rodent-borne hantaviruses endemic in Europe. Among about 800 sequences tiled on the microarray, 52 correspond to a tight molecular sieve of hantavirus probes covering a large genetic landscape. RNAs from infected animal tissues or from laboratory strains have been reverse transcribed, amplified, then hybridized to the microarray. A classical BLASTN analysis applied to the sequence delivered through the microarray allows to identify the hantavirus species up to the exact geographical variant present in the tested samples. Geographical variants of the most common European hantaviruses from France, Germany, Slovenia and Finland, such as Puumala virus, Dobrava virus and Tula virus, were genetically discriminated. Furthermore, we precisely characterized geographical variants still unknown when the chip was conceived, such as Seoul virus isolates, recently emerged in France and the United Kingdom.
Non-Contact Universal Sample Presentation for Room Temperature Macromolecular Crystallography Using Acoustic Levitation
Authors: Morris R. H. , Dye E. R. , Axford D. , Newton M. I. , Beale J. H. , Docker P. T. 
Published: August 2019

AbstractMacromolecular Crystallography is a powerful and valuable technique to assess protein structures. Samples are commonly cryogenically cooled to minimise radiation damage effects from the X-ray beam, but low temperatures hinder normal protein functions and this procedure can introduce structural artefacts. Previous experiments utilising acoustic levitation for beamline science have focused on Langevin horns which deliver significant power to the confined droplet and are complex to set up accurately. In this work, the low power, portable TinyLev acoustic levitation system is used in combination with an approach to dispense and contain droplets, free of physical sample support to aid protein crystallography experiments. This method facilitates efficient X-ray data acquisition in ambient conditions compatible with dynamic studies. Levitated samples remain free of interference from fixed sample mounts, receive negligible heating, do not suffer significant evaporation and since the system occupies a small volume, can be readily installed at other light sources.
Complex Susceptibilities and Chiroptical Effects of Collagen Measured with Polarimetric Second-Harmonic Generation Microscopy
Authors: Golaraei Ahmad , Kontenis Lukas , Mirsanaye Kamdin , Krouglov Serguei , Akens Margarete K. , Wilson Brian C. , Barzda Virginijus 
Published: August 2019

AbstractNonlinear optical properties of collagen type-I are investigated in thin tissue sections of pig tendon as a research model using a complete polarimetric second-harmonic generation (P-SHG) microscopy technique called double Stokes-Mueller polarimetry (DSMP). Three complex-valued molecular susceptibility tensor component ratios are extracted. A significant retardance is observed between the chiral susceptibility component and the achiral components, while the achiral components appear to be in phase with each other. The DSMP formalism and microscopy measurements are further used to explain and experimentally validate the conditions required for SHG circular dichroism (SHG-CD) of collagen to occur. The SHG-CD can be observed with the microscope when: (i) the chiral second-order susceptibility tensor component has a non-zero value, (ii) a phase retardance is present between the chiral and achiral components of the second-order susceptibility tensor and (iii) the collagen fibres are tilted out of the image plane. Both positive and negative areas of SHG-CD are observed in microscopy images, which relates to the anti-parallel arrangement of collagen fibres in different fascicles of the tendon. The theoretical formalism and experimental validation of DSMP imaging technique opens new opportunities for ultrastructural characterisation of chiral molecules, in particular collagen, and provides basis for the interpretation of SHG-CD signals. The nonlinear imaging of chiroptical parameters offers new possibilities to further improve the diagnostic sensitivity and/or specificity of nonlinear label-free histopathology.
Long-term isolation at a low effective population size greatly reduced genetic diversity in Gulf of California fin whales
Authors: Rivera-León Vania E. , Urbán Jorge , Mizroch Sally , Brownell Robert L. , Oosting Tom , Hao Wensi , Palsbøll Per J. , Bérubé Martine 
Published: August 2019

AbstractThe Gulf of California, Mexico is home to many cetacean species, including a presumed resident population of fin whales,Balaenoptera physalus. Past studies reported very low levels of genetic diversity among Gulf of California fin whales and a significant level of genetic differentiation from con-specifics in the eastern North Pacific. The aim of the present study was to assess the degree and timing of the isolation of Gulf of California fin whales in a population genetic analysis of 18 nuclear microsatellite genotypes from 402 samples and 565 mitochondrial control region DNA sequences (including mitochondrial sequences retrieved from NCBI). The analyses revealed that the Gulf of California fin whale population was founded ~2.3 thousand years ago and has since remained at a low effective population size (~360) and isolated from the eastern North Pacific (Nembetween 0.89–1.4). The low effective population size and high degree of isolation implied that Gulf of California fin whales are vulnerable to the negative effects of genetic drift, human-caused mortality and habitat change.
Differentiating cervical metastatic lymphadenopathy and lymphoma by shear wave elastography
Authors: Chae Soo Young , Jung Hye Na , Ryoo Inseon , Suh Sangil 
Published: August 2019

AbstractShear wave elastography (SWE) is a recent technological advance of ultrasonography (US) to assess tissue stiffness. The purpose of this study is to assess tissue stiffness of malignant cervical lymph nodes (LN) with SWE, to reveal diagnostic performance of SWE in differentiating metastatic LN from lymphoma, and to assess inter-observer agreement of SWE. We assessed 62 malignant LN (24 lymphomas and 38 metastatic LN) and their median speed was 6.34 m/s and median elasticity was 69.7 kPa. Add of SWE with conventional US improved diagnostic accuracy of differentiating metastasis from lymphoma (16.13, 8.07 and 11.3% for three radiologists). Kendall’s Coefficient of Concordance of three readers for analyzing SWE patterns was 0.86. SWE can be a useful tool to discriminate metastatic cervical LN from lymphoma with improvement of diagnostic accuracy when using with conventional US.
Phylogenetic characterization of bacterial endophytes from four Pinus species and their nematicidal activity against the pine wood nematode
Authors: Ponpandian Lakshmi Narayanan , Rim Soon Ok , Shanmugam Gnanendra , Jeon Junhyun , Park Young-Hwan , Lee Sun-Keun , Bae Hanhong 
Published: August 2019

AbstractRecently, bacterial endophytes (BEs) have gained importance in the agricultural sector for their use as biocontrol agents to manage plant pathogens. Outbreak of the pine wilt disease (PWD) in Korea has led researchers to test the feasibility of BEs in controlling the pine wood nematode (PWN)Bursaphelenchus xylophilus. In this study, we have reported the diversity and biocontrol activity of BEs against the PWN. By employing a culture-dependent approach, 1,622 BEs were isolated from the needle, stem, and root tissues ofP.densiflora,P.rigida,P.thunbergii, andP.koraiensisacross 18 sampling sites in Korea. We classified 389 members based on 16S rDNA analysis and taxonomic binning, of which, 215 operational taxonomic units (OTUs) were determined. Using Shannon’s indices, diversity across thePinusspecies and tissues was estimated to reveal the composition of BEs and their tissue-specific preferences. When their ethyl acetate crude extracts were analysed for biocontrol activity, 44 candidates with nematicidal activity were obtained. Among these,StenotrophomonasandBacillussp. exhibited significant inhibitory activity against PWN during their developmental stages. Altogether, our study furnishes a basic comprehension of bacterial communities found in thePinusspecies and highlights the potential of BEs as biocontrol agents to combat PWD.
PIDD interaction with KEAP1 as a new mutation-independent mechanism to promote NRF2 stabilization and chemoresistance in NSCLC
Authors: Ji Lili , Zhang Rui , Chen Jie , Xue Qun , Moghal Nadeem , Tsao Ming-Sound 
Published: August 2019

AbstractChemotherapy resistance is a major problem in non-small cell lung cancer (NSCLC) treatment. A major mechanism of chemoresistance involves stabilization of the NRF2 transcription factor. NRF2 levels are normally tightly regulated through interaction with KEAP1, an adaptor that targets NRF2 to the CUL3 E3 ubiquitin ligase for proteolysis. In NSCLC, aberrant NRF2 stabilization is best understood through mutations in NRF2, KEAP1, or CUL3 that disrupt their interaction. Biochemical studies, however, have revealed that NRF2 can also be stabilized through expression of KEAP1-interacting proteins that competitively sequester KEAP1 away from NRF2. Here, we have identified PIDD, as a novel KEAP1-interactor in NSCLC that regulates NRF2. We show that this interaction allows PIDD to reduce NRF2 ubiquitination and increase its stability. We also demonstrate that PIDD promotes chemoresistance in NSCLC cells both in vitro and in vivo, and that this effect is dependent on NRF2. Finally, we report that NRF2 protein expression in a NSCLC cohort exceeds the typical incidence of combined NRF2, KEAP1, and CUL3 mutations, and that NRF2 expression in this cohort is correlated with PIDD levels. Our data identify PIDD as a new NRF2 regulator, and suggest that variations in PIDD levels contribute to differential chemosensitivities among NSCLC patients.