bluuebunny/crossref_metadata_2025_split huggingface dataset:
HCV and HBV prevalence based on home blood self-sampling and screening history in the general population in 2016: contribution to the new French screening strategy
Authors: Brouard Cécile , Saboni Leïla , Gautier Arnaud , Chevaliez Stéphane , Rahib Delphine , Richard Jean-Baptiste , Barin Francis , Larsen Christine , Sommen Cécile , Pillonel Josiane , Delarocque-Astagneau Elisabeth , Lydié Nathalie , Lot Florence 
Published: August 2019

Abstract
Background

The advent of effective direct-acting antivirals (DAAs), has prompted an assessment of the French Hepatitis C virus (HCV) screening strategy, which historically targeted high-risk groups. One of the options put forward is the implementation of combined (i.e., simultaneous) HCV, Hepatitis B virus (HBV) and HIV screening for all adults at least once during their lifetime (“universal combined screening”). However, national population-level data are lacking to guide decision-making regarding which new strategy to implement. Accordingly, we aimed to provide updated data for both chronic hepatitis C (CHC) and B (CHB) prevalence and for HCV and HBV screening history, using data from the BaroTest and 2016 Health Barometer (2016-HB) studies, respectively.

Methods

2016-HB was a national cross-sectional phone based health survey conducted in 2016 among 20,032 randomly selected individuals from the general population in metropolitan France. BaroTest was virological sub-study nested in 2016-HB. Data collected for Barotest were based on home blood self-sampling on dried blood spots (DBS).

Results

From 6,945 analyzed DBS, chronic hepatitis C (CHC) and B (CHB) prevalence was estimated at 0.30% (95% Confidence Interval (CI): 0.13-0.70) and 0.30% (95% CI: 0.13-0.70), respectively. The proportion of individuals aware of their status was estimated at 80.6% (95% CI: 44.2-95.6) for CHC and 17.5% (95% CI: 4.9-46.4) for CHB. Universal combined screening would involve testing between 32.6% and 85.3% of 15-75 year olds according to whether we consider only individuals not previously tested for any of the three viruses, or also those already tested for one or two of the viruses.

Conclusions

Our data are essential to guide decision-making regarding which new HCV screening recommendation to implement in France. They also highlight that efforts are still needed to achieve the WHO’s targets for eliminating these diseases. Home blood self-sampling may prove to be a useful tool for screening and epidemiological studies.
The predictive prognostic factors and risk factors of lymph node metastasis in patients with early gastric cancer
Authors: zu hongliang , Wang huiling , MA yan , xue yingwei 
Published: August 2019

Abstract
Background: Determining the prognosis of early gastric cancer (EGC) is very important for the selection of preoperative treatment strategies. The purpose of this paper was to investigate the clinicopathological features and prognostic factors in EGC and the related risk factors of lymph node metastasis (LNM).
Methods: From March 2007 through December 2010, 1004 patients who underwent gastrectomy at Harbin Medical University were retrospectively identified; 120 patients were diagnosed with EGC. The clinicopathological features and prognostic factors were analysed by univariate and multivariate analyses. Multivariate logistic regression analysis was used to discern risk factors for LNM in EGC.
Results: The incidence of EGC was 11.96%. A univariate analysis showed that age, preoperative haemoglobin (Hb) level, prealbumin level, tumour size and LNM were significant prognostic factors. A multivariate analysis showed that the preoperative Hb level and LNM were independent prognostic factors. A multivariate logistic regression analysis revealed that age, Ca-199 level and macroscopic tumour type were independent risk factors for LNM in EGC.
Conclusions: Preoperative Hb level and LNM were both independent prognostic factors for EGC. These factors may help surgeons implement appropriate treatment strategies during the perioperative period.
Retrospective analysis of the efficacy and safety of rivaroxaban in the treatment of hepatic sinus obstruction syndrome caused by Gynura segetum
Authors: Bing Hao , Li Dan , Chang Bing , Li Yiling , He Xiangmin , Tong Jing , Wang Ying , Ao Ran , Wang Ningning 
Published: August 2019

Abstract
Background

PA-HSOS is a rare disease and there is no specific treatment for PA-HSOS. Anticoagulant, antithrombotic and microcirculation therapy can alleviate the progression of PA-HSOS. The application of rivaroxaban in patients with PA-HSOS has not yet been reported. The aim of this study was to analyze the efficacy and safety of rivaroxaban in the treatment of pyrrolizidine alkaloid-induced hepatic sinusoidal obstruction syndromecaused by Gynura segetum (PA-HSOS) .

Methods

A retrospective analysis was conducted with the clinical data of patients with PA-HSOS in the acute/subacute phase caused by taking Gynura segetum. The patients were divided into warfarin and rivaroxaban groups according to the anticoagulant therapy they received. Related biochemical indicators were monitored during hospitalization. Liver ultrasound, liver elastography and related biochemical indicators were reviewed every two weeks or one month after discharge. The patients were followed until 1 year after complete remission or death. The efficacy and safety of rivaroxaban was compared with that of warfarin according to the patients’ hepatic venous recanalization rates and the occurrence of bleeding events.

Results

The study included 20 patients, with 10 patients in the warfarin group and 10 patients in the rivaroxaban group. The results showed that the average anticoagulant course in the rivaroxaban group was significantly shorter than that in the warfarin group (P=0.007). With treatment, the remission rates of the rivaroxaban group and the warfarin group reached 90%. There was no significant difference in the incidence of adverse reactions or bleeding events between the two groups (P>0.05).

Conclusions

Compared with warfarin, rivaroxaban, a new oral anticoagulant, is convenient and safe for clinical use. It has an obvious effect on PA-HSOS and a low risk of bleeding. It provides a new anticoagulant treatment for PA-HSOS.
Thioredoxin system protein expression in carcinomas of the pancreas, bile duct and ampulla.
Authors: Al-hadyan Khaled S. , Storr Sarah J. , Zaitoun Abed M. , Lobo Dileep N. , Martin Stewart G. 
Published: August 2019

Abstract

Background: Pancreatic cancer (PC), including the ampulla and bile duct, is very aggressive, and thus difficult to treat with effective therapies. The current treatment options have failed to improve PC five-year survival rates over the last 30 to 40 years, which remain very low, at ~3%; there is, therefore, an urgent need to identify new targets and treatment modalities (1).
Methods: The protein expression of thioredoxin (Trx), thioredoxin reductase (TrxR) and thioredoxin interacting protein (TxNIP) was assessed in two cancer patient cohorts by standard immunohistochemistry using tissue microarrays. The first cohort was composed of 85 pancreatic adenocarcinomas (PAD) and the second of 145 cancers of the bile duct and ampulla.
Results: In the PAD cohort, high cytoplasmic TrxR expression significantly associated with lymph node metastasis (P = 0.033). High expression of cytoplasmic (P = 0.018) and nuclear (P = 0.006) Trx were significantly associated with better overall survival, with nuclear Trx expression remaining significantly associated with survival in multivariate Cox-regression (Hazard Ratio (HR) 0.316; 95% Confidence Interval (95% CI) 0.174-0.573; P < 0.0001) when potentially confounding factors were included (gender, age, tumour size, tumour grade, tumour stage, lymph node status, perineural and venous invasion).
In cancers of the bile duct and ampulla, high expression of nuclear TrxR and high cytoplasmic TxNIP were associated with patients aged above 60 years (P = 0.024 and P = 0.049 respectively). Associations were also observed between high nuclear TrxR expression and the presence of venous (P = 0.001) and perineural (P = 0.021) invasion. Low cytoplasmic TxNIP expression was also associated with the presence of perineural invasion (P = 0.025). High expression of cytoplasmic TxNIP was significantly associated with better overall survival (P = 0.0002), which remained significant in multivariate Cox-regression analysis (HR 0.548; 95% CI 0.340-0.882; P = 0.013) when potentially confounding factors were included (tumour grade, stage, lymph node status, perineural and venous invasion).
Conclusion: Current findings demonstrate the prognostic importance of Trx system protein expression in pancreatic, bile duct and ampullary cancers, with expression of certain members potentially being involved in disease progression. Current findings warrant a larger follow-up study.
lncRNA ABHD11-AS1 drives gastric cancer development by targeting miR-1301-3p/PDPK1 signaling
Authors: xin hairong , yan zhefeng , cao jie 
Published: August 2019

Abstract
Background

Long noncoding RNAs (lncRNAs) has long been reported to associate with multitudinous kinds of cancer. The ABHD11-AS1, a newly identified lncRNAs, has been demonstrated as a new marker of gastric cancer (GC). In this study, a significant upregulation of ABHD11-AS1 was observed in GC, compared to adjacent normal tissues.

Method

Immunohistochemical analysis for ABHD11-AS1, 3-phosphoinositide dependent protein kinase 1 (PDPK1), miR-1301-3p was performed on gastric tumor and adjacent normal tissues. The gene expression was evaluated by qRT-PCR. The proliferation was assayed by CCK8 and Annexin V/PI assay.

Results

ABHD11-AS1 is highly expressed in GC tissues compared with adjacent normal tissues(*p<0.01) Knock-down of ABHD11-AS1 could suppress the aberrant proliferation of GC cells compared with si-Scramble(*p<0.01). LncRNA-ABHD11-AS1 served as a sponge for miR-1301-3p in GC cell. miR-1301-3p/ABHD11-AS1 regulated the expression of PDPK1 in GC cells compared with control *p<0.01). miR-1301-3p/ ABHD11-AS1 co-mediated proliferation and apoptosis of GC cell compared with si-Scramble/Mock and si-ABHD11-AS1/Mock (*p<0.01)

Conclusion

PDPK1 was a downstream target of miR-133a-3p and ABHD11-AS1 subsequently exerted its biological effects via modulating the expression of PDPK1in gastric cancer cells.

Key

lncRNA,ABHD11-AS1, gastric cancer, miR-1301-3p, PDPK1
The prognostic value of B7-H6 in esophageal squamous cell carcinoma
Authors: Yang Shu , Zhou Huan , Dong Jun , Guo Liyi , Wang Xicheng , Wang Kailin , Cai Xiuyu 
Published: August 2019

Abstract
Background

B7-h6, a member of the B7 family molecules, participates in the clearance of tumor cells by binding to NKp30 on NK cells. The expression of B7-H6 in esophageal squamous cell carcinoma and the clinical significance is unknown. The goal of this study was to determine the expression of B7-H6 in esophageal squamous cell carcinoma and the clinical significance of B7-H6 expression.

Patients and methods

We retrospectively collected clinical data from 145 patients diagnosed with esophageal squamous cell carcinoma between January 2007 and December 2008. These patients had all previously undergone surgical treatment for esophageal cancer, were clearly diagnosed, and had not received chemotherapy or radiotherapy. In addition, pathological tissue samples from the 145 patients were collected to detect the expression of B7-H6 by immunohistochemistry. The chi-square (χ2) test was used to analyse the relationships between B7-H6 and clinicopathological characteristics. The prognosis of the patients were analysed by Cox proportional hazards regression analysis and Kaplan-Meier analysis.

Results

B7-H6 was present in 133/145 (91.72%) of the esophageal squamous cell carcinoma samples and all localized in the cytoplasm. The expression level of B7-H6 was correlated with T stage (P=0.036) and lymphatic metastasis status (P=0.044). According to the results of the ROC curve analysis, H-score =90 was selected as the cut-off value. The 145 patients were divided into two groups, the high B7-H6 expression (H-score>90) group and the low B7-H6 expression (H-score≤90) group. Cox proportional hazards regression analysis indicated that tumour size (P=0.021), B7-H6 expression (P=0.025) and lymphatic metastasis status (P=0.049) were independent prognostic factors for esophageal squamous cell carcinoma. Kaplan-Meier analysis with the log-rank test demonstrated that the patients with high B7-H6 expression (P = 0.003), lymphatic metastasis (P <0.001) or a tumour size ≥ 3.0 cm (P = 0.001) had significantly worse survival than those with low B7-H6 expression, no lymphatic metastasis or a tumour size < 3.0 cm respectively.

Conclusion

B7-H6 is widely expressed in esophageal squamous cell carcinoma and high expression of B7-H6 can be used as a predictor of poor prognosis.
Production and assessment of polyclonal antibody against the whole virion of Goatpox virus and sheeppox virus in the rabbit
Authors: Jing Zhizhong , Hurisa Takele Tesgera , Jia Huaijie , Chen Guohua , Xiang Fang Yong , He Xiao-Bing , Wang Xia oxia 
Published: August 2019

Abstract

Abstract
Background: Antibodies have emerged as essential tools of biomedical researches and are of great commercial and medical values. They are the fastest growing product segments of the pharmaceutical industry. Polyclonal antibodies are antibodies that secreted by different B cell lineages within the body. In this study production of polyclonal antibody against Goatpox and Sheeppox virion in rabbit was performed and subsequently; it will be used as the development of ELISA for detection of poxvirus in different species of animals.
Results: After 0.5mg/ml of the whole virion of the poxvirus was injected subcutaneously at multiple sites, the blood was collected at an interval of 14 days and antibody titration was conducted. GTPV A27 antigen was coated and indirect ELISA method was used for the titration of antibody then the mean of OD values for positive and negative results were analyzed by Microsoft excel window 7. The blood was collected and serum was prepared for IgG purification. The IgG was purified by Ammonium sulfate precipitation method which was dialyzed against 5lire PBS overnight at +4oc. The protein concentration was determined spectrophotometrically at 280nm wavelength and estimated as 2.29µg/µl and 2.18µg/µl against Goatpox virus and sheeppox virus respectively. Protein bands were checked by SDS PAGE and the molecular weights of 67kDa and 25kDas were estimated for IgG. similarly, antigen-antibody detection was checked by western blot.
Conclusions: Based on the results of this study, the virion of Goatpox virus and Sheeppox virus is strong enough to produce antibody to develop ELISA kit for detection of poxviruses by coating GPTV A27 antigen. This study is the first to be conducted for the production of antibody from virions of Goatpox and sheeppox virus which was tittered by coating GTPV A27 antigen.
Improvement and Use of CRISPR/Cas9 to Engineer a Sperm-marking Strain for the Invasive Fruit Pest Drosophila suzukii
Authors: Ahmed Hassan M. M. , Hildebrand Luisa , Wimmer Ernst A. 
Published: August 2019

Abstract
Background: The invasive fruit pest Drosophila suzukii has been reported for the first time in Europe and USA in 2008 and has spread since then. The adoption of type II clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) as a tool for genome manipulation has opened the doors for researchers to develop novel biotechnologically based pest control approaches. Stage and or tissue specific genes are of particular importance in the field of insect biotechnology. The enhancer/promoter of the spermatogenesis specific beta-2-tubulin (β2t) gene was used to drive the expression of fluorescent proteins or effector molecules in testes of agricultural pests and diseases vectors for sexing, monitoring and reproductive biology studies. Here, we demonstrate an improvement in the efficiency of CRISPR/Cas-based genome editing in D. suzukii and establish a sperm-marking system.Results: To improve genome editing we isolated and tested the D. suzukii endogenous promoters of the small nuclear RNA gene U6 to drive the expression of a guide RNA and the Ds heat shock protein 70 promoter to express Cas9. As a comparison, we used recombinant Cas9 protein and in vitro transcribed gRNA as a preformed ribonucleoprotein. To evaluate the homology-dependent repair (HDR)-based genome editing efficiency, we used as a target platform a previously established transgenic line that expresses DsRed ubiquitously. In addition, we isolated Ds_β2t gene and used its promoter to drive the expression of a red fluorescence protein in the sperm. A transgenic sperm-marking strain was then established by the improved HDR-based genome editing.Conclusion: The use of the endogenous promoters of D. suzukii U6 and hsp70 genes to drive the expression of gRNA and Cas9, respectively, showed improved efficiency of gene editing compared to previous reports. The sperm-marking system should help to monitor the success of pest control campaigns in the context of the Sterile Insect Technique and provides a tool for basic research in reproductive biology of this invasive pest. Furthermore, the promoter of the β2t gene can be used in developing novel transgenic pest control approaches. The CRISPR/Cas9 system can be used as an additional tool for the modification of previously established transgenes.
Transcriptome profiling of the elongating internode of cotton (Gossypium hirsutum L.) seedlings in response to mepiquat chloride
Authors: Wang Li , Yin Ying , Wang Li-Feng , Wang Menglei , Zhao Miao , Tian Ye , Li Yong-Fang 
Published: August 2019

Abstract
Background

The plant growth retardant mepiquat chloride (MC) has been extensively used to produce compact plant canopies and increase yield in cotton (Gossypium hirsutum L.). Previous studies mainly focused on the role of gibberellins (GA) in MC-induced growth inhibition of cotton. However, the molecular mechanism underlying MC-induced growth retardation has remained largely unknown.

Results

In the present study, we conducted histological, transcriptomic, and phytohormone analyses of the second elongating internodes of cotton seedlings treated with MC. Histological analysis revealed that the MC-mediated shortening of internodes was caused by the suppression of cell division and decrease in cell length; this phenotype was confirmed by transcriptome profiling. Many genes related to cell growth were significantly downregulated in MC-treated internodes, such as cell cycle, cell wall biosynthesis and modification, and transport protein aquaporins. Furthermore, the expression of genes related to secondary metabolism, especially lignin and flavonoid, was down-regulated by MC treatment. The expression of genes related to GA, auxin, brassinosteroid (BR), and ethylene metabolism and signaling was remarkably suppressed, whereas that of genes related to cytokinin (CK) and abscisic acid (ABA) metabolism was induced by MC. Consistent with RNA-Seq analysis, significant decrease in endogenous GA, auxin, and BR content, but an increase in CK content, was observed in cotton internodes after MC treatment. In addition, many transcription factors (TFs) such as bHLH, AP2-EREBP, Orphans, MYB, GRF, and TCP were differentially regulated by MC; these TFs are associated with cell division and expansion, phytohormone signaling, and circadian rhythm.

Conclusions

This study provides novel insights into the molecular mechanism underlying the MC-mediated inhibition of internode elongation in cotton seedlings. MC reduces internode elongation by suppressing the biosynthesis and downstream signaling cascades of GA, auxin, and BR; altering the expression of many TFs; and further reducing cell division and expansion.
The effects of food simulating liquids on surface roughness, hardness, and solubility of bulk fill composites
Authors: Cabadağ Özge Gizem , GÖNÜLOL NİHAN 
Published: August 2019

Abstract
BackgroundThe aim of this study was to compare the effects of food simulating liquids (FSLs) on the surface roughness, surface hardness and solubility of bulk fill composites. MethodsTwo-hundred samples were prepared from four high viscosity bulk fill composites (SonicFillTM, Tetric® EvoCeram Bulk Fill, Beautifil-Bulk Restorative, FiltekTM Bulk Fill Posterior Restorative) and a microhybrid composite (FiltekTM Z250). After the initial weights of the samples were recorded (m1), surface roughness measurements were evaluated with a profilometer. The samples were stored in 4 different FSLs for 7 days, then the second surface roughness values were recorded. Then the samples were stored in a desiccator to reach the constant mass and the values were recorded as m2. The solubility levels were calculated according to the ISO 4049: 2009 specification. The surface hardness values of the samples were determined by using the Vickers microhardness measuring device. Twenty specimens from each group were evaluated in terms of surface morphology with scanning electron microscope (SEM).ResultsBeautifil Bulk Restorative was the most affected group in terms of surface roughness after storage in FSLs and citric acid caused the highest values in this group (p<0,005). Beautifil Bulk Restorative and Filtek Z250 groups showed the highest surface hardness values, while the Tetric EvoCeram group had the lowest. The highest solubility values were detected in Beautifil Bulk Restorative group, and citric acid and ethanol caused the highest solubility values on all the tested composites. ConclusionsBeautifil Bulk Restorative group showed significant differences from the other groups in all tested parameters.
Oral Impact on Daily Performance (OIDP) scale for use in Sri Lankan Adolescents: Modification and validation of a Sinhalese version
Authors: Amilani Uttara , Jayasekara Prasanna , Perera Irosha R , Carter Hannah E , Senanayake Sameera , Kularatna Sanjeewa 
Published: August 2019

Abstract
Background Oral Health Related Quality of Life (OHRQoL) surveys play an important role in understanding subjective patient experiences in oral health care. The Oral Impact on Daily Performance (OIDP) scale is a validated OHRQoL tool that measures the impact and extent to which an individual’s daily activities may be compromised by their oral health. It is commonly used to facilitate oral health service planning. The aim of this study was to modify and validate a Sinhalese version of the OIDP for use in Sri Lankan adolescents. Methods The first stage involved cultural adaptation of the tool through translation and modification. After translation and cultural adaptation, the modified OIDP was tested on 220, 15-19 year old adolescents in Sri Lanka. The adolescents completed the modified OIDP scale together with the questions evaluation their self-rated oral health as a self-administered questionnaire. This involved the exploring factor structure, validation and a reliability assessment. Factorability was assessed by inspection of correlation matrix and Kaiser-Meyer-Olkin and Bartlett's Test of Sphericity tests as a measure of sampling adequacy. An exploratory factor analysis was carried out using Principal Component Analysis method and factors were rotated using the oblimin method. Results 220 adolescents participated in factor analysis and validation studies. The Kaiser-Meyer-Olkin measure was 0.87 and Bartlett’s test of Sphericity was significant (p<0.001) Cronbach’s alpha was calculated as 0.88, indicating a high level of internal consistency. The principal component analysis produced two factors with Eigen values ranging from 1.12 to 4.40, explaining 70.0% of total variance. Concurrent validity was satisfactory as the OIDP score increased when the adolescents’ perceived oral health decreased. Conclusion This study showed that the modified OIDP scale is applicable for use among adolescents in Sri Lanka. It has promising psychometric properties but further research is required to use this tool in other cohorts.