bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization of a murine model of non-lethal, symptomatic dengue virus infection
Authors: Sarathy Vanessa V. , White Mellodee , Li Li , Kaiser Jaclyn A. , Campbell Gerald A. , Milligan Gregg N. , Bourne Nigel , Barrett Alan D. T. 
Published: March 2018

AbstractThe mosquito-borne disease dengue is caused by four serologically- and genetically-related viruses, termed DENV-1 to DENV-4. Historical setbacks due to lack of human-like mouse models of dengue were partially remedied with characterization of lethal DENV-2 infection in immunocompromised AG129 mice (deficient in IFN-α/β/γ receptors). Recently, our group established lethal AG129 mouse infection models of DENV-1, DENV-3, and DENV-4 using human isolates. Here we compare a non-lethal, disseminated model of DENV-3 infection using strain D83-144 to that of the lethal outcome following infection by strain C0360/94. Both strains belong to DENV-3 genotype II and differ by only 13 amino acids. Intraperitoneal inoculation of AG129 mice with strain D83-144 led to clinical signs of dengue infection, such as cytokine induction, thrombocytopenia, and systemic infection. However, C0360/94 infection led to features of severe human dengue, including coagulopathy and lethal outcome, whereas D83-144 infection does not. This study is the first to investigate a low passage, non-mouse lethal strain in AG129 mice and demonstrates that D83-144 infection induces milder features of human dengue than those induced by lethal C0360/94 infection. The results suggest that the AG129 mouse model has applications to investigate factors associated with mild or severe disease.
In vitro effects of hydroxyapatite containing toothpastes on dentin permeability after multiple applications and ageing
Authors: Hiller Karl-Anton , Buchalla Wolfgang , Grillmeier Isabel , Neubauer Christina , Schmalz Gottfried 
Published: March 2018

AbstractThisin vitrostudy evaluated the effect of toothpastes with different active ingredients on dentin permeability using an extended protocol including multiple applications and several thermal ageing cycles in the presence or absence of human saliva. The Null hypothesis was that dentin permeability of a hydroxyapatite containing toothpaste (BR), a potassium nitrate (SP) and an arginine and calcium carbonate (EH) containing toothpaste were similar. Dentin permeability was measured as hydraulic conductance using a commercially available capillary flow system (Flodec, Geneva) and results were expressed as % relative to matching controls. Without saliva, the ranking (best first) of dentin permeability was BR(61%) < SP(87%) < EH(118%), with saliva EH(63%) < SP(72%) < BR(88%). Saliva increased or decreased permeability dependent upon the test material. BR reduced dentin permeability significantly more in absence of saliva, with saliva EH was superior to BR. Repeated material application decreased and thermal ageing increased dentin permeability. The different tooth pastes reduced permeability differently, the best being BR without saliva, the least EH without saliva. The newly introduced test conditions (ageing, saliva, multiple applications) influenced single results significantly, and as they better simulate thein vivosituation they should be considered to be included in furtherin vitropermeability testing of desensitizing preparations.
Solar cyclic variability can modulate winter Arctic climate
Authors: Roy Indrani 
Published: March 2018

AbstractThis study investigates the role of the eleven-year solar cycle on the Arctic climate during 1979–2016. It reveals that during those years, when the winter solar sunspot number (SSN) falls below 1.35 standard deviations (or mean value), the Arctic warming extends from the lower troposphere to high up in the upper stratosphere and vice versa when SSN is above. The warming in the atmospheric column reflects an easterly zonal wind anomaly consistent with warm air and positive geopotential height anomalies for years with minimum SSN and vice versa for the maximum. Despite the inherent limitations of statistical techniques, three different methods – Compositing, Multiple Linear Regression and Correlation – all point to a similar modulating influence of the sun on winter Arctic climate via the pathway of Arctic Oscillation. Presenting schematics, it discusses the mechanisms of how solar cycle variability influences the Arctic climate involving the stratospheric route. Compositing also detects an opposite solar signature on Eurasian snow-cover, which is a cooling during Minimum years, while warming in maximum. It is hypothesized that the reduction of ice in the Arctic and a growth in Eurasia, in recent winters, may in part, be a result of the current weaker solar cycle.
Below the Mesophotic
Authors: Baldwin C. C. , Tornabene L. , Robertson D. R. 
Published: March 2018

AbstractMesophotic coral ecosystems, which occur at depths of ~40 to 150 m, have received recent scientific attention as potential refugia for organisms inhabiting deteriorating shallow reefs. These ecosystems merit research in their own right, as they harbor both depth-generalist species and a distinctive reef-fish fauna. Reef ecosystems just below the mesophotic are globally underexplored, and the scant recent literature that mentions them often suggests that mesophotic ecosystems transition directly into those of the deep sea. Through submersible-based surveys in the Caribbean Sea, we amassed the most extensive database to date on reef-fish diversity between ~40 and 309 m at any single tropical location. Our data reveal a unique reef-fish assemblage living between ~130 and 309 m that, while taxonomically distinct from shallower faunas, shares strong evolutionary affinities with them. Lacking an existing name for this reef-faunal zone immediately below the mesophotic but above the deep aphotic, we propose “rariphotic.” Together with the “altiphotic,” proposed here for the shallowest reef-faunal zone, and the mesophotic, the rariphotic is part of a depth continuum of discrete faunal zones of tropical reef fishes, and perhaps of reef ecosystems in general, all of which warrant further study in light of global declines of shallow reefs.
Prediction of influenza-like illness based on the improved artificial tree algorithm and artificial neural network
Authors: Hu Hongping , Wang Haiyan , Wang Feng , Langley Daniel , Avram Adrian , Liu Maoxing 
Published: March 2018

AbstractBecause influenza is a contagious respiratory illness that seriously threatens public health, accurate real-time prediction of influenza outbreaks may help save lives. In this paper, we use the Twitter data set and the United States Centers for Disease Control’s influenza-like illness (ILI) data set to predict a nearly real-time regional unweighted percentage ILI in the United States by use of an artificial neural network optimized by the improved artificial tree algorithm. The results show that the proposed method is an efficient approach to real-time prediction.
Improved NO2 Gas Sensing Properties of Graphene Oxide Reduced by Two-beam-laser Interference
Authors: Guo Li , Hao Ya-Wei , Li Pei-Long , Song Jiang-Feng , Yang Rui-Zhu , Fu Xiu-Yan , Xie Sheng-Yi , Zhao Jing , Zhang Yong-Lai 
Published: March 2018

AbstractWe report on the fabrication of a NO2 gas sensor from room-temperature reduction of graphene oxide(GO) via two-beam-laser interference (TBLI). The method of TBLI gives the distribution of periodic dissociation energies for oxygen functional groups, which are capable to reduce the graphene oxide to hierarchical graphene nanostructures, which holds great promise for gaseous molecular adsorption. The fabricated reduced graphene oxide(RGO) sensor enhanced sensing response in NO2 and accelerated response/recovery rates. It is seen that, for 20 ppm NO2, the response (Ra/Rg) of the sensor based on RGO hierarchical nanostructures is 1.27, which is higher than that of GO (1.06) and thermal reduced RGO (1.04). The response time and recovery time of the sensor based on laser reduced RGO are 10 s and 7 s, which are much shorter than those of GO (34 s and 45 s), indicating that the sensing performances for NO2 sensor at room temperature have been enhanced by introduction of nanostructures. This mask-free and large-area approach to the production of hierarchical graphene micro-nanostructures, could lead to the implementation of future graphene-based sensors.
Identification of di-substituted ureas that prevent growth of trypanosomes through inhibition of translation initiation
Authors: Machado Fabricio Castro , Franco Caio Haddad , dos Santos Neto Jose Vitorino , Dias-Teixeira Karina Luiza , Moraes Carolina Borsoi , Lopes Ulisses Gazos , Aktas Bertal Huseyin , Schenkman Sergio 
Published: March 2018

AbstractSome 1,3-diarylureas and 1-((1,4-trans)−4-aryloxycyclohexyl)−3-arylureas (cHAUs) activate heme-regulated kinase causing protein synthesis inhibition via phosphorylation of the eukaryotic translation initiation factor 2 (eIF2) in mammalian cancer cells. To evaluate if these agents have potential to inhibit trypanosome multiplication by also affecting the phosphorylation of eIF2 alpha subunit (eIF2α), we tested 25 analogs of 1,3-diarylureas and cHAUs against Trypanosoma cruzi, the agent of Chagas disease. One of them (I-17) presented selectivity close to 10-fold against the insect replicative forms and also inhibited the multiplication of T. cruzi inside mammalian cells with an EC50 of 1–3 µM and a selectivity of 17-fold. I-17 also prevented replication of African trypanosomes (Trypanosoma brucei bloodstream and procyclic forms) at similar doses. It caused changes in the T. cruzi morphology, arrested parasite cell cycle in G1 phase, and promoted phosphorylation of eIF2α with a robust decrease in ribosome association with mRNA. The activity against T. brucei also implicates eIF2α phosphorylation, as replacement of WT-eIF2α with a non-phosphorylatable eIF2α, or knocking down eIF2 protein kinase-3 by RNAi increased resistance to I-17. Therefore, we demonstrate that eIF2α phosphorylation can be engaged to develop trypanosome-static agents in general, and particularly by interfering with activity of eIF2 kinases.
Comparison of Clinical Efficacy of Lateral and Lateral and Medial Double-plating Fixation of Distal Femoral Fractures
Authors: Bai Zhibiao , Gao Shichang , Hu Zhenming , Liang Anlin 
Published: March 2018

AbstractThe present study was performed to compare the clinical efficacy of lateral plate and lateral and medial double-plating fixation of distal femoral fractures and explore the indication of lateral and medial double-plating fixation of the distal femoral fractures. From March 2006 to April 2014, 48 and 12 cases of distal femoral fractures were treated with lateral plate (single plate) and lateral and medial plates (double plates), respectively. During the surgery, after setting the lateral plate for the distal femoral fractures, if the varus stress test of the knee was positive and the lateral collateral ligament rupture was excluded, lateral and medial double-plating fixation was used for the stability of the fragments. All the patients were followed up at an average period of 15.9 months. The average operation time, the intraoperative hemorrhage and the fracture union time of the two groups were compared. One year after operation, knee function was evaluated by the Kolmert’s standard. There was no significant difference in the average operation time, intraoperative hemorrhage, fracture healing time and excellent and good rates of postoperative knee function between two groups. Positive Varus stress test during operation can be an indication for lateral and medial double-plating fixation of distal femoral fractures.
Significant production of humic fluorescent dissolved organic matter in the continental shelf waters of the northwestern Pacific Ocean
Authors: Kim Jeonghyun , Cho Hyung-Mi , Kim Guebuem 
Published: March 2018

AbstractFluorescent dissolved organic matter (FDOM) is important for marine organisms and the global carbon cycle contributing to the optical properties of surface seawater and organic carbon budgets. Rivers are known to be the main source of FDOM in coastal oceans and marginal seas. In this study, however, we show that the contribution of FDOM produced from organic sediments of the northwestern Pacific continental shelf is similar to that from the Changjiang River. FDOM showed relatively higher concentrations at stations off the Changjiang River mouth and in the central Yellow Sea. Based on temperature-salinity diagrams, the major source of surface FDOM in summer surface waters was found to be from the Changjiang River while that observed in the winter water column was produced mainly in the continental shelf. A good correlation between 228Ra and the humic-like FDOM (FDOMH) during the winter suggests that the FDOMH is produced mainly from marine sediments and enriched in water over the water residence times. A simple mass balance calculation shows that the excess FDOMH fluxes produced from marine sediments account for 30–40% of the riverine source. This result suggests that the continental shelf is an important hidden source of FDOM in the upper ocean.
Executive control processes are associated with individual fitness outcomes following regular exercise training: blood lactate profile curves and neuroimaging findings
Authors: Pensel M. C. , Daamen M. , Scheef L. , Knigge H. U. , Rojas Vega S. , Martin J. A. , Schild H. H. , Strüder H. K. , Boecker H. 
Published: March 2018

AbstractCardiovascular training has been associated with neuroimaging correlates of executive control functions (ECF) in seniors and children/adolescents, while complementary studies in middle-aged populations are lacking. Ascribing a prominent role to cardiorespiratory fitness improvements, most studies concentrated on training-induced gains in maximal oxygen uptake (VO2max), although other fitness indices may provide complementary information. Here, we investigated the impact of long-term sub-maximal exercise training on interference control, considering individual training-induced shifts in blood lactate profile curves (BLC) and VO2max. Twenty-three middle-aged sedentary males (M = 49 years) underwent a six-month exercise program (intervention group, IG). Additionally, 14 individuals without exercise training were recruited (control group, CG, M = 52 years). Interference control was assessed before and after the intervention, using a functional magnetic resonance imaging (fMRI) flanker paradigm. Task performance and brain activations showed no significant group-by-time interactions. However, regression analyses in the IG revealed significant associations between individual fitness gains and brain activation changes in frontal regions, which were not evident for VO2max, but for BLC. In conclusion, training-induced plasticity of ECF-related brain activity can be observed in late middle adulthood, but depends on individual fitness gains. For moderate training intensities, BLC shifts may provide sensitive markers for training-induced adaptations linked to ECF-related brain function.
Mask Responses for Single-Pixel Terahertz Imaging
Authors: Augustin Sven , Frohmann Sven , Jung Peter , Hübers Heinz-Wilhelm 
Published: March 2018

AbstractTerahertz (THz) radiation meaning electromagnetic radiation in the range from 0.1 (3) to 10 (30) has the unique advantage of easily penetrating many obstructions while being non-hazardous to organic tissue since it is non-ionizing. A shortcoming of this domain is the limited availability of high-sensitivity detector arrays respective THz cameras with >1k pixels. To overcome the imaging limitations of the THz domain, compressive imaging in combination with an optically controllable THz spatial light modulator is a promising approach especially when used in a single-pixel imaging modality. The imaging fidelity, performance and speed of this approach depend crucially on the imaging patterns also called masks and their properties used in the imaging process. Therefore, in this paper, it is investigated how the image quality after reconstruction is specifically influenced by the different mask types and their properties in a compressive imaging modality. The evaluation uses an liquid-crystal display based projector as spatial light modulator to derive specific guidelines for the use of binary and true greyscale masks in THz single-pixel imaging setups respective THz single-pixel cameras when used in far-field applications e.g. stand-off security imaging.
Inter-patient image registration algorithms to disentangle regional dose bioeffects
Authors: Monti Serena , Pacelli Roberto , Cella Laura , Palma Giuseppe 
Published: March 2018

AbstractRadiation therapy (RT) technological advances call for a comprehensive reconsideration of the definition of dose features leading to radiation induced morbidity (RIM). In this context, the voxel-based approach (VBA) to dose distribution analysis in RT offers a radically new philosophy to evaluate local dose response patterns, as an alternative to dose-volume-histograms for identifying dose sensitive regions of normal tissue. The VBA relies on mapping patient dose distributions into a single reference case anatomy which serves as anchor for local dosimetric evaluations. The inter-patient elastic image registrations (EIRs) of the planning CTs provide the deformation fields necessary for the actual warp of dose distributions. In this study we assessed the impact of EIR on the VBA results in thoracic patients by identifying two state-of-the-art EIR algorithms (Demons and B-Spline). Our analysis demonstrated that both the EIR algorithms may be successfully used to highlight subregions with dose differences associated with RIM that substantially overlap. Furthermore, the inclusion for the first time of covariates within a dosimetric statistical model that faces the multiple comparison problem expands the potential of VBA, thus paving the way to a reliable voxel-based analysis of RIM in datasets with strong correlation of the outcome with non-dosimetric variables.
Simplifying research access to genomics and health data with Library Cards
Authors: Cabili Moran N. , Carey Knox , Dyke Stephanie O. M. , Brookes Anthony J. , Fiume Marc , Jeanson Francis , Kerry Giselle , Lash Alex , Sofia Heidi , Spalding Dylan , Tasse Anne-Marie , Varma Susheel , Pandya Ravi 
Published: March 2018

AbstractThe volume of genomics and health data is growing rapidly, driven by sequencing for both research and clinical use. However, under current practices, the data is fragmented into many distinct datasets, and researchers must go through a separate application process for each dataset. This is time-consuming both for the researchers and the data stewards, and it reduces the velocity of research and new discoveries that could improve human health. We propose to simplify this process, by introducing a standard Library Card that identifies and authenticates researchers across all participating datasets. Each researcher would only need to apply once to establish their bona fides as a qualified researcher, and could then use the Library Card to access a wide range of datasets that use a compatible data access policy and authentication protocol.
Cell–cell interactions between monocytes/macrophages and synoviocyte-like cells promote inflammatory cell infiltration mediated by augmentation of MCP-1 production in temporomandibular joint
Authors: Ibi Miho , Horie Sawa , Kyakumoto Seiko , Chosa Naoyuki , Yoshida Mariko , Kamo Masaharu , Ohtsuka Masato , Ishisaki Akira 
Published: March 2018

Many inflammatory cells are known to be home to inflamed temporomandibular joint (TMJ) tissues by stimulation with cytokines and chemokines produced by inflammatory lesions in the TMJ. However, how the inflammatory cells affect the progression of inflammation in TMJ synovial tissues after their homing to inflamed TMJ site is still uncertain. Here, we isolated and cultured TMJ synoviocyte-like cells (TMJSCs) from murine TMJ tissues. We demonstrated that interleukin 1β (IL-1β) up-regulated expression of monocyte chemoattractant protein 1 (MCP-1) in TMJSCs. In addition, we found that IL-1β-treated TMJSCs strongly promoted migratory activity of mouse monocyte/macrophage RAW264.7 cells through secretion of MCP-1. On the other hand, IL-1β up-regulated expression levels of intracellular adhesion molecule 1 (ICAM-1), a leukocyte adhesion ligand in TMJSCs. In addition, IL-1β promoted cell–cell adhesion between TMJSCs and RAW264.7 cells. Intriguingly, we also found that cell–cell interactions mediated through soluble factors other than IL-1β and cell–cell adhesion molecules between IL-1β-stimulated TMJSCs and RAW264.7 cells synergistically augmented secretion of MCP-1 from these cells. Therefore, these results suggested that the IL-1β-induced recruitment of monocyte/macrophage lineage cells to inflamed synovial membranes in TMJ was further augmented by the cell–cell interaction-induced secretion of MCP-1 from the inflammation site, possibly resulting in prolonged inflammatory responses in TMJ synovial tissue.
Serum, liver and bile sitosterol and sitostanol in obese patients with and without NAFLD
Authors: Tauriainen Milla-Maria , Männistö Ville , Kaminska Dorota , Vaittinen Maija , Kärjä Vesa , Käkelä Pirjo , Venesmaa Sari , Gylling Helena , Pihlajamäki Jussi 
Published: March 2018

Background and aims: Non-alcoholic fatty liver disease (NAFLD) associates with low levels of serum plant sterols in cross-sectional studies. In addition, it has been suggested that the hepatic sterol transport mechanisms are altered in NAFLD. Therefore, we investigated the association between serum, liver and bile plant sterols and sitostanol with NAFLD.:
MethodsOut of the 138 individuals (age: 46.3 ± 8.9, body mass index: 43.3 ± 6.9 kg/m², 28% men and 72% women), 44 could be histologically categorized to have normal liver, and 94 to have NAFLD. Within the NAFLD group, 28 had simple steatosis and 27 had non-alcoholic steatohepatitis. Plant sterols and sitostanol were measured from serum (n=138), liver (n=38), and bile (n=41). The mRNA expression of genes regulating liver sterol metabolism and inflammation was measured (n=102).:
ResultsLiver and bile sitostanol ratios to cholesterol were higher in those with NAFLD compared to those with histologically normal liver (all P&lt;0.022). Furthermore, liver sitostanol to cholesterol ratio correlated positively with histological steatosis and lobular inflammation (rs &gt; 0.407, P&lt;0.01 for both). In contrast, liver sitosterol to cholesterol ratio correlated negatively with steatosis (rs = −0.392, P=0.015) and lobular inflammation (rs = −0.395, P=0.014). Transcriptomics analysis revealed suggestive correlations between serum plant sterol levels and mRNA expression.:
ConclusionOur study showed that liver and bile sitostanol ratios to cholesterol associated positively and liver sitosterol ratio to cholesterol associated negatively with liver steatosis and inflammation in obese individuals with NAFLD..
Capacitor‐reused CMOS temperature sensor with duty‐cycle‐modulated output and 0.38°C () inaccuracy
Authors: Tang Zhong , Fang Yun , Yu Xiaopeng , Shi Zheng , Tan Nianxiong 
Published: March 2018

A new bipolar junction transistor‐based temperature sensor with duty‐cycle‐modulated output is presented. By employing a capacitor‐reused voltage to the duty‐cycle converter, the proposed design achieves both area and energy efficiencies simultaneously. The self‐controlled chopping, dynamic element matching and digital‐assisted calibration techniques are applied as well to further improve the accuracy. Fabricated in a standard 0.13 μm CMOS process, the proposed sensor achieves the measured 3 inaccuracy of from to after one‐point calibration while occupying a silicon area of 0.073 mm2.