bluuebunny/crossref_metadata_2025_split huggingface dataset:
CD9 in acute myeloid leukemia: Prognostic role and usefulness to target leukemic stem cells
Authors: Touzet Lucas , Dumezy Florent , Roumier Christophe , Berthon Céline , Bories Claire , Quesnel Bruno , Preudhomme Claude , Boyer Thomas 
Published: February 2019

AbstractCD9 is a cell surface protein and belongs to the tetraspanin family. Its role in carcinomagenesis has been widely studied in solid tumors but remains controversial, depending on the cancer type. Although CD9 seems to be associated with unfavorable outcome and disease progression in acute lymphoblastic leukemia (ALL), this marker has not yet been studied in acute myeloid leukemia (AML). First, we explored its prognostic role and its association with biological factors in a cohort of 112 AML patients treated with intensive chemotherapy. CD9 was expressed in 40% of AML and was associated with a favorable outcome (event‐free survival and relapse‐free survival) in univariate (P = 0.009 and P = 0.048, respectively) and multivariate (P = 0.004 and P = 0.039, respectively) analyses. Interestingly, CD9 expression was different between the more immature physiologic and AML cells (CD34+CD38−) as it was also expressed in AML on putative leukemic stem cells (LSCs) but not on hematopoietic stem cells (HSCs). Hence, CD9 could be a very relevant marker for minimal residual disease (MRD) monitoring in AML based on LSC targeting.
The average relative dose intensity of R‐CHOP is an independent factor determining favorable overall survival in diffuse large B‐cell lymphoma patients
Authors: Długosz‐Danecka Monika , Szmit Sebastian , Ogórka Tomasz , Skotnicki Aleksander B. , Jurczak Wojciech 
Published: February 2019

AbstractThe prognosis of diffuse large B‐cell lymphoma (DLBCL) patients depends on lymphoma‐ and patient‐related risk factors and is best estimated by the international prognostic index (IPI). The aim of the study was to determine whether the average relative dose intensity (ARDI) of an anthracycline‐containing regimen could predict DLBCL outcome independently from the IPI. We analyzed 223 white Caucasian DLBCL patients who completed at least four cycles of first‐line immunochemotherapy with rituximab, doxorubicin, cyclophosphamide, vincristine, and prednisone (R‐CHOP). The ARDI was calculated by specially developed software in each individual patient, simultaneously with the chemotherapy prescription, which instantly revealed all causes of its decrease. The relevance of the ARDI for progression‐free/overall survival (PFS/OS) was evaluated. Prolonged intervals between cycles of immunochemotherapy—the most common cause of decreased ARDI (49.3%, 110/223)—were due to neutropenia (absolute neutrophil count <1.0 × 109/L) and infections. Reductions in cytostatic doses were observed in 19.7% (44/223) of patients, mainly as the consequence of cardiotoxicity (23/223, 10.3%). The OS varied significantly when the ARDI was >90% (P < 0.00001). Multivariate analysis confirmed that an ARDI>90% was an IPI‐independent predictor of prolonged PFS (HR = 0.31; 95%CI: 0.20‐0.47; P < 0.00001) and OS (HR = 0.32; 95%CI: 0.21‐0.48; P < 0.00001). With an analytic tool allowing real‐time ARDI assessment, it was possible to maintain an ARDI above 90% in 161 of 223 patients (72%). DLBCL patients with an ARDI >90% have significantly better outcome regardless of the IPI; therefore, our official recommendation is an adequate dose density through efficient neutropenia prophylaxis and cardiac protection.
Isoalantolactone inhibits IKKβ kinase activity to interrupt the NF‐κB/COX‐2‐mediated signaling cascade and induces apoptosis regulated by the mitochondrial translocation of cofilin in glioblastoma
Authors: Xing Jin‐Shan , Wang Xun , Lan Yu‐Long , Lou Jia‐Cheng , Ma Binbin , Zhu Tingzhun , Zhang Hongqiang , Wang Dongsheng , Yu Zhikuan , Yuan Zhongbo , Li Xin‐Yu , Zhang Bo 
Published: February 2019

AbstractIsoalantolactone (IATL), a sesquiterpene lactone compound, possesses many pharmacological and biological activities, but its role in glioblastoma (GBM) treatment is still unknown. The aim of the current study was to investigate the antiglioma effects of IATL and to explore the underlying molecular mechanisms. In the current study, the biological functions of IATL were examined by MTT, cell migration, colony formation, and cell apoptosis assays. Confocal immunofluorescence techniques, chromatin immunoprecipitation, and pull‐down assays were used to explore the precise underlying molecular mechanisms. To examine IATL activity and the molecular mechanisms by which it inhibits glioma growth in vivo, we used a xenograft tumor mouse model. Furthermore, Western blotting was used to confirm the changes in protein expression after IATL treatment. According to the results, IATL inhibited IKKβ phosphorylation, thus inhibiting both the binding of NF‐κB to the cyclooxygenase 2 (COX‐2) promoter and the recruitment of p300 and eventually inhibiting COX‐2 expression. In addition, IATL induced glioma cell apoptosis by promoting the conversion of F‐actin to G‐actin, which in turn activates the cytochrome c (Cyt c) and caspase‐dependent apoptotic pathways. In the animal experiments, IATL reduced the size and weight of glioma tumors in xenograft mice and inhibited the expression of COX‐2 and phosphorylated NF‐κB p65 in the transplanted tumors. In conclusion, the current study indicated that IATL inhibited the expression of COX‐2 through the NF‐κB signaling pathway and induced the apoptosis of glioma cells by increasing actin transformation. These results suggested that IATL could be greatly effective in GBM treatment.
Non‐acute promyelocytic leukemia variant, acute myeloid leukemia with translocation (11;17)
Authors: Altahan Rahaf , Altahan Shatha , Khalil Salem 
Published: February 2019

Key Clinical Messaget(11;17) is a rare but recognized finding usually found in Acute Promyelocytic Leukemia with variant RARA translocation (APLv). We present a case of Acute Myeloid Leukemia with t(11;17) that has different break points than those occurring in APLv. The diagnosis of acute myeloid leukemia, not otherwise specified, acute monoblastic leukemia was reached after a thorough investigation. Reaching the correct diagnosis and distinguishing these two entities are essential as they have different management, prognosis, and overall survival.
Modeling of High‐Pressure Ethene Homo‐ and Copolymerization
Authors: Peikert Paul , Pflug Kristina M. , Busch Markus 
Published: February 2019

AbstractWhile reaction engineering of low‐molecular weight compounds mainly focuses on equilibria and selectivities, polymer properties are tremendously influenced by molecular weight distribution as well as branching structure. In order to determine the branching structure of low‐density polyethylene (LDPE) copolymers in dependence on chosen process conditions, a Monte‐Carlo approach was developed. By modeling the topology as well as the comonomer distribution in the polymer chains a deeper insight in the process‐microstructure‐properties relationship is gained.
Breast cancer deaths averted over 3 decades
Authors: Hendrick R. Edward , Baker Jay A. , Helvie Mark A. 
Published: February 2019

BackgroundFrom 1975 to 1990, female breast cancer mortality rates in the United States increased by 0.4% per year. Since 1990, breast cancer mortality rates have fallen between 1.8% and 3.4% per year, a decrease that is attributed to increased mammography screening and improved treatment.MethodsThe authors used age‐adjusted female breast cancer mortality rate and population data from the Surveillance, Epidemiology, and End Results (SEER) program to estimate the number of breast cancer deaths averted by screening mammography and improved treatment since 1989. Four different assumptions regarding background mortality rates (in the absence of screening mammography and improved treatment) were used to estimate deaths averted for women aged 40 to 84 years by taking the difference between SEER‐reported mortality rates and background mortality rates for each 5‐year age group, multiplied by the population for each 5‐year age group. SEER data were used to estimate annual and cumulative breast cancer deaths averted in 2012 and 2015 and extrapolated SEER data were used to estimate deaths averted in 2018.ResultsThe number of single‐year breast cancer deaths averted ranged from 20,860 to 33,842 in 2012, from 23,703 to 39,415 in 2015, and from 27,083 to 45,726 in 2018. Breast cancer mortality reductions ranged from 38.6% to 50.5% in 2012, from 41.5% to 54.2% in 2015, and from 45.3% to 58.3% in 2018. Cumulative breast cancer deaths averted since 1989 ranged from 237,234 to 370,402 in 2012, from 305,934 to 483,435 in 2015, and from 384,046 to 614,484 in 2018.ConclusionsSince 1989, between 384,000 and 614,500 breast cancer deaths have been averted through the use of mammography screening and improved treatment.
Water as an Effective Additive for High‐Energy‐Density Na Metal Batteries? Studies in a Superconcentrated Ionic Liquid Electrolyte
Authors: Ferdousi Shammi A. , Hilder Matthias , Basile Andrew , Zhu Haijin , O'Dell Luke A. , Saurel Damien , Rojo Teofilo , Armand Michel , Forsyth Maria , Howlett Patrick C. 
Published: February 2019

AbstractThe effect of water on the properties of superconcentrated sodium salt solutions in ionic liquids (ILs) was investigated to design electrolytes for sodium battery applications with water as an additive. Water was added to a 50 mol % solution of NaFSI [FSI=bis(fluorosulfonyl)imide] in the ionic liquid N‐methyl‐N‐propylpyrrolidinium bis(fluorosulfonyl)imide (C3mpyrFSI). Although the thermal properties (e.g., glass transition temperature) showed little dependence on the water content, the viscosity and, in particular, the ionic conductivity were strongly affected. The Na|Na symmetrical cell cycling performance was strongly dependent on the applied current density as well as on the water content. At higher current densities (1.0 mA cm−2) the polarization profiles showed a water dependence, suggesting that water was actively involved in the formation of an improved solid electrolyte interface layer (SEI) for high‐water‐content samples (1000–5000 ppm), resulting in improved long‐term cycling stability. The initial impedance of cells cycled at 1.0 mA cm−2 (measured after 20 cycles) was elevated after water addition, and large polarizations occured for the “wet” samples. However, with further cycling the wet cells began to exhibit lower polarization and improved stability compared to the “dry” sample. The Na|NaFePO4 cell cycling performance was also demonstrated with minimal effect on the cell capacity, further highlighting the negligible activity of water in these electrolyte systems. In fact, reduced cell polarization and a more clearly defined charge profile were evident after water addition. The work shown here suggests that water may be used as a convenient and inexpensive additive for superconcentrated sodium IL electrolytes for improved device performance.
Natural Deep Eutectic Solvents as Multifunctional Media for the Valorization of Agricultural Wastes
Authors: Ma Yunjian , Li Peilin , Li Yongru , Willot Sébastien J.‐P. , Zhang Wuyuan , Ribitsch Doris , Choi Young Hae , Verpoorte Robert , Zhang Tianyu , Hollmann Frank , Wang Yonghua 
Published: February 2019

AbstractThe use of natural deep eutectic solvents (NADES) as multifunctional solvents for limonene bioprocessing was reported. NADES were used for the extraction of limonene from orange peel wastes, as solvent for the chemoenzymatic epoxidation of limonene, and as sacrificial electron donor for the in situ generation of H2O2 to promote the epoxidation reaction. The proof‐of‐concept for this multifunctional use was provided, and the scope and current limitations of the concept were outlined.
Innovations in biological assessments of chronic stress through hair and nail cortisol: Conceptual, developmental, and methodological issues
Authors: Liu Cindy H. , Doan Stacey N. 
Published: February 2019

AbstractMuch of the existing research on biological mechanisms underlying the stress experience has focused largely on moment‐to‐moment stress, rather than on chronic stress, an arguably more powerful predictor of long‐term outcomes. Recent methodological innovations have paved the way for new lines of research on chronic stress, with promising implications for developmental researchers and for those who study health and adversity. In particular, there are increasing studies that have focused on chronic stress assessments by relying on cortisol derived from hair and nails as a biomarker for chronic stress. In this paper, we provide an overview of their use, describe how hair and nail cortisol ought to be conceptualized differently across the lifespan, how developmental factors may impact its interpretation, and the circumstances under which its use may be more methodologically sensible. The purpose of this review is to provoke further discussion and encourage careful research designs that utilize hair and nail cortisol for understanding the effects of chronic stress exposure from the early developmental period, across adverse contexts, and in association with psychological and physical health outcomes.
Aggregation, segregation, and dispersal of homotypic germ plasm RNPs in the early zebrafish embryo
Authors: Eno Celeste , Hansen Christina L. , Pelegri Francisco 
Published: February 2019

BackgroundIn zebrafish and many other organisms, specification of primordial germ cells (PGCs) requires the transmission of maternally‐derived germ plasm. Zebrafish germ plasm ribonucleoparticles (RNPs) aggregate along the cleavage furrows during the first several cell cycles, segregate asymmetrically during the cleavage stages, and undergo cytoplasmic dispersal in the late blastula.ResultsFor all tested germ plasm RNAs [carbonic anhydrase 15b (ca15b), deleted in azoospermia‐like (dazl), dead end (dnd), nanos 3 (nos3), regulator of G‐protein signaling14a (rgs14a), and vasa/DEAD box polypeptide 4 (vasa/ddx4)], RNPs are homotypic (containing a single RNA type), with RNPs packing tightly yet remaining distinct within germ plasm aggregates. Homotypic clustering of RNAs within RNPs is observed before aggregation in the cortex and is maintained through germ plasm recruitment, asymmetric segregation and RNP dispersal. We also identify a step of germ plasm fragmentation during the cleavage stages that precedes RNP dispersal.ConclusionsOur findings suggest that germ plasm aggregates act as subcellular compartments that temporarily collect and carry single RNA‐type RNPs from fertilization until their cytoplasmic dispersal in PGCs at the end of the blastula period, and describe a previously unknown fragmentation step that allows for an increase in the pool of germ plasm‐carrying cells, presumably PGCs. Developmental Dynamics 248:306–318, 2019. © 2019 Wiley Periodicals, Inc.
Long‐term follow‐up study of low‐weight avoidant restrictive food intake disorder compared with childhood‐onset anorexia nervosa: Psychiatric and occupational outcome in 56 patients
Authors: Lange C.R. André , Ekedahl Fjertorp Hanna , Holmer Riitta , Wijk Elin , Wallin Ulf 
Published: February 2019

AbstractObjectiveTo compare long term outcome between childhood‐onset Anorexia Nervosa (AN) and low‐weight Avoidant/Restrictive Food Intake Disorder (ARFID) in regard to psychiatric diagnoses, social and occupational functioning.MethodA consecutive series of 56 children originally treated for low‐weight restrictive eating disorder (ED) were followed up after a mean of 15.9 years. ARFID‐diagnoses were assigned retrospectively.ResultsThirty‐seven patients originally had AN and 19 patients were diagnosed retrospectively with ARFID. At follow‐up, in the AN‐group 21.6% had a current ED, 24.3% had another psychiatric diagnosis, and 54.1% did not have any psychiatric diagnosis. In the ARFID‐group, 26.3% had a current ED, 26.3% had another psychiatric diagnosis, and 47.4% had no psychiatric diagnosis. In the ARFID‐group ED diagnoses at follow‐up were all ARFID, whereas the AN‐group showed heterogeneity. Morgan Russell Outcome Assessment Schedule indicated similar outcome in the AN‐ and ARFID‐group. Occupational functioning did not differ significantly between the AN‐ and ARFID‐group.DiscussionThe AN‐group showed high rate of ED at follow up. The ARFID‐group had a similar outcome to AN. In the ARFID‐group, all ED‐cases at follow up had ARFID, possibly indicating symptomatic stability. Low‐weight ARFID should be treated as seriously as childhood onset AN.
Phenotypic plasticity of four Chenopodiaceae species with contrasting saline–sodic tolerance in response to increased salinity–sodicity
Authors: Huang Yingxin , Fan Gaohua , Zhou Daowei , Pang Jiayin 
Published: February 2019

AbstractIt is unknown whether phenotypic plasticity in fitness‐related traits is associated with salinity–sodicity tolerance. This study compared growth and allocation phenotypic plasticity in two species with low salinity–sodicity tolerance (Chenopodium acuminatum and C. stenophyllum) and two species with high salinity–sodicity tolerance (Suaeda glauca and S. salsa) in a pot experiment in the Songnen grassland, China. While the species with low tolerance had higher growth and allocation plasticity than the highly tolerant species, the highly tolerant species only adjusted their growth traits and maintained higher fitness (e.g., plant height and total biomass) in response to increased soil salinity–sodicity, with low biomass allocation plasticity. Most plasticity is “apparent” plasticity (ontogenetic change), and only a few traits, for example, plant height:stem diameter ratio and root:shoot biomass ratio, represent “real” plasticity (real change in response to the environment). Our results show that phenotypic plasticity was negatively correlated with saline–sodic tolerance and could be used as an index of species sensitivity to soil salinity–sodicity.
Water salinity and inundation control soil carbon decomposition during salt marsh restoration: An incubation experiment
Authors: Wang Faming , Kroeger Kevin D. , Gonneea Meagan E. , Pohlman John W. , Tang Jianwu 
Published: February 2019

AbstractCoastal wetlands are a significant carbon (C) sink since they store carbon in anoxic soils. This ecosystem service is impacted by hydrologic alteration and management of these coastal habitats. Efforts to restore tidal flow to former salt marshes have increased in recent decades and are generally associated with alteration of water inundation levels and salinity. This study examined the effect of water level and salinity changes on soil organic matter decomposition during a 60‐day incubation period. Intact soil cores from impounded fresh water marsh and salt marsh were incubated after addition of either sea water or fresh water under flooded and drained water levels. Elevating fresh water marsh salinity to 6 to 9 ppt enhanced CO2 emission by 50%−80% and most typically decreased CH4 emissions, whereas, decreasing the salinity from 26 ppt to 19 ppt in salt marsh soils had no effect on CO2 or CH4 fluxes. The effect from altering water levels was more pronounced with drained soil cores emitting ~10‐fold more CO2 than the flooded treatment in both marsh sediments. Draining soil cores also increased dissolved organic carbon (DOC) concentrations. Stable carbon isotope analysis of CO2 generated during the incubations of fresh water marsh cores in drained soils demonstrates that relict peat OC that accumulated when the marsh was saline was preferentially oxidized when sea water was introduced. This study suggests that restoration of tidal flow that raises the water level from drained conditions would decrease aerobic decomposition and enhance C sequestration. It is also possible that the restoration would increase soil C decomposition of deeper deposits by anaerobic oxidation, however this impact would be minimal compared to lower emissions expected due to the return of flooding conditions.
Different roles of elevational and local environmental factors on abundance‐based beta diversity of the soil Enchytraeidae on the Changbai Mountain
Authors: Jiang Xiaoming , Chen Jing , Xie Zhicai 
Published: February 2019

AbstractThe elevational alpha biodiversity gradient in mountain regions is one of the well‐known ecological patterns, but its beta diversity pattern remains poorly known. Examining the beta diversity and its components could enhance the understanding of community assembly mechanism. We studied the beta diversity pattern of the soil enchytraeids along a distinct elevational gradient (705–2,280 m) on the Changbai Mountain, the best‐preserved mountain in northeastern China. The overall abundance‐based community dissimilarity was relatively high (ca. 0.70), largely due to the balanced‐variation component (85%). The overall dissimilarity and its balanced‐variation (substitution) component were related to both local environmental heterogeneity and elevational distance, with the environmental relationships being stronger. In contrast, the abundance‐gradient (subsets) component was not related to the two gradients. The same important spatial and environmental variables were detected in structuring overall dissimilarity and substitution component, different from that in subsets component. Variation partitioning analysis showed that environmental control played a more important role than spatial (vertical and horizontal) factors in structuring the patterns of overall beta diversity and its two components. The predictive power of multivariate analysis was higher for the substitution component (nearly 50%) and overall dissimilarity (35%), but much lower for subsets components (<4%). These findings implied that abundance‐based beta diversity patterns of the soil enchytraeids were the results of different ecological processes (e.g., environmental sorting and dispersal limitation), operating in the two antithetic components. Our study showed the substitution and loss of individuals reflecting different ecological processes and highlights the importance of partitioning beta diversity in assessing biodiversity patterns and their causes.
Structure and genetic variability of golden mussel (Limnoperna fortunei) populations from Brazilian reservoirs
Authors: Furlan‐Murari Pâmela Juliana , Ruas Claudete de Fatima , Ruas Eduardo Augusto , Benício Lucas Milanez , Urrea‐Rojas Angela Maria , Poveda‐Parra Angela Rocio , Murari Emerson , Lima Ed Christian Suzuki de , Souza Felipe Pinheiro de , Lopera‐Barrero Nelson Mauricio 
Published: February 2019

AbstractThe golden mussel, Limnoperna fortunei a highly invasive species in Brazil, has generated productive, economical, and biological impacts. To evaluate genetic structure and variability of L. fortunei populations present in fish farms in the reservoirs of Canoas I (CANFF), Rosana (ROSFF), and Capivara (CAPFF) (Paranapanema River, Paraná, Brazil), eight microsatellite loci were amplified. Five of those eight loci resulted in 38 alleles. The observed heterozygosity (Ho) was lower than the expected heterozygosity (He) in all populations, with a deviation from the Hardy–Weinberg equilibrium (HWE). The average value for the inbreeding coefficient (Fis) was positive and significative for all populations. There was higher genetic variability within populations than among them. The fixation index (Fst) showed a small genetic variability among these populations. The occurrence of gene flow was identified in all populations, along with the lack of a recent bottleneck effect. The clustering analysis yielded K = 2, with genetic similarity between the three populations. The results demonstrate low genetic structure and suggest a founding population with greater genetic variability (ROSFF). Our data point to the possible dispersal of L. fortunei aided by anthropic factors in the upstream direction. It was concluded that the three populations presented a unique genetic pool for Paranapanema River, with occurrence of gene flow.