bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synthesis, Molecular Properties Prediction and Antimicrobial Activity of Imidazolyl Schiff Bases, Triazoles and Azetidinones
Authors: Rekha Tamatam , Nagarjuna Ummadi , Padmaja Adivireddy , Padmavathi Venkatapuram 
Published: April 2019

AbstractBenzylidenehydrazinyl imidazoles (3) are prepared from 2‐hydrazinyl imidazoles (2) on treatment with hydrazine. The imine functionality in 3 is utilized to develop 5′‐aryl‐N‐(4‐aryl‐1H‐imidazol‐2‐yl)‐1H‐1,2,3‐triazol‐1‐amines (5) by 1,3‐dipolar cycloaddition of diazomethane followed by aromatization with I2 in DMSO. Compounds 3 are also explored to prepare 4′‐aryl‐1‐(4‐aryl‐1H‐imidazol‐2‐ylamino)‐3‐chloroazetidin‐2‐ones (6) on treatment with chloroacetyl chloride. The Molinspiration calculations predicted that 3, 5 and 6 have molecular hydrophobicity, conformational flexibility, good intestinal absorption and bioactivity scores. The chloro, bromo and nitro substituted imidazolyl azetidinones (6c, 6d, 6f) and nitro substituted imidazolyl triazole (5f) exhibited excellent antibacterial activity on B. subtilis, whereas chloro and nitro substituted imidazolyl triazoles (5c, 5f) showed prominent antifungal activity on A. niger.
VitaFlow™ transcatheter valve system in the treatment of severe aortic stenosis: One‐year results of a multicenter study
Authors: Zhou Daxin , Pan Wenzhi , Wang Jianan , Wu Yongjian , Chen Mao , Modine Thomas , Mylotte Darren , Piazza Niccolo , Ge Junbo 
Published: April 2019

AbstractObjectiveThis study reports the 1‐year clinical outcomes of the VitaFlow™ transcatheter aortic valve system in the treatment of severe aortic stenosis.BackgroundThe VitaFlow™ system (MicroPort®, Shanghai, China) was developed as a novel transcatheter aortic valve replacement system to mitigate or circumvent some of the challenges associated with heavily calcified valves and bicuspid valves.MethodsFrom September 2014 to November 2017, a prospective, multicenter, single arm study was conducted in 11 centers in China. The primary end point was all cause mortality at 12 months.ResultsOne hundred and ten symptomatic aortic stenosis patients (60 men, 50 women; mean age 77.73 ± 4.78 years) at prohibitive or high risk for surgery were enrolled. Mean society of thoracic surgeons score was 8.84 ± 5.58%. All‐cause mortality was 2.7% at 1‐year. Major stroke, major vascular complication, coronary artery obstruction, new pacemaker implantation occurred in 2.7, 2.7, 1.8, and 19.1% at 1‐year follow‐up, respectively. No patients had moderate or severe paravalvular leak at 1‐year. At 1 year follow‐up, 97% of patients had New York heart association ≤II. Patients with bicuspid valves had similar outcomes as those patients with tricuspid aortic valve stenosis.ConclusionsThe 12‐month clinical results support the safety and efficacy of VitaFlow™ in the treatment of patients with severe aortic stenosis, including patients with bicuspid aortic valve.
Coronary lithotripsy for failed rotational atherectomy, cutting balloon, scoring balloon, and ultra‐high‐pressure non‐compliant balloon
Authors: Venuti Giuseppe , D'Agosta Guido , Tamburino Corrado , La Manna Alessio 
Published: April 2019

AbstractCalcified and undilatable stenosis still represents a challenge in percutaneous coronary interventions (PCI), due to the higher risk of suboptimal result with consequent worse clinical outcomes. Unfortunately, the dedicated technologies and devices, such as specialized balloon and atherectomy systems, do not always provide adequate plaque modification and optimal vessel preparation allowing optimal stent delivery. The intravascular lithotripsy (IVL) is a technology derived from urology that has been tested in peripheral and coronary calcified plaques, with promising preliminary results. We present a case of a patient undergoing planned PCI of the right coronary artery targeting an undilatable lesion, already resistant to both specialized balloons and rotational atherectomy. Using the IVL system, we were able to break the calcium, guarantying optimal stent expansion with good final result.
Conservatively managed saddle pulmonary embolism
Authors: Kristensen Anna Meta Dyrvig , Rosberg Victoria , Juel Jacob , Pareek Manan 
Published: April 2019

AbstractMassive, submassive, and nonmassive pulmonary embolism relate to the hemodynamic state, while saddle pulmonary embolus is a purely radiologic term. Patients with saddle embolus often present with hemodynamic compromise. However, treatment depends on the clinical presentation, and stable patients with a saddle pulmonary embolus can respond well to conventional anticoagulation.
Switchable C−H Alkylation of Aromatic Acids with Maleimides in Water: Carboxyl as a Diverse Directing Group
Authors: Pu Fan , Liu Zhong‐Wen , Zhang Lin‐Yan , Fan Juan , Shi Xian‐Ying 
Published: April 2019

AbstractA ruthenium‐catalyzed protocol to access conjugate addition or decarboxylative conjugate addition of aromatic acids with maleimides has been developed. The reaction shows interesting chemoselectivity with different substituted benzoic acids. The reaction pathway of C−H alkylation is controlled by the intrinsic property of aromatic acids but not reaction conditions. Under almost the same reaction conditions, carboxyl can act as either a classical directing group or a traceless directing group, thereby generating two kinds of products, i. e., 2‐alkyl substituted benzoic acids and alkyl substituted benzenes. These two reactions proceeded under mild and redox‐neutral conditions in neat water under the atmosphere of air, and could be easily scaled up to grams. The decarboxylative conjugate addition, where carboxyl acts as a traceless directing group, can be realized without the addition of any ligand, silver or copper salt.
Enzyme Immobilization in MOF‐derived Porous NiO with Hierarchical Structure: An Efficient and Stable Enzymatic Reactor
Authors: Gao Xia , Ding Yu , Sheng Yude , Hu Mancheng , Zhai Quanguo , Li Shuni , Jiang Yucheng , Chen Yu 
Published: April 2019

AbstractMOF‐derived porous NiO with hierarchical structure (MHNiO) was prepared based on thermolysis of Ni metal organic framework (Ni‐MOF), which was used as carrier for the immobilization of horseradish peroxidase (HRP) and cytochrome C (Cyt c). The pore size of MHNiO was tuned as 11.8 nm to match the size of free enzyme so as to depress the aggregation of the enzymes in the pore. Meanwhile, the hierarchical structure allowed the substrates concentrated in the vicinity of the enzymes. The obtained enzymatic reactors exhibited better thermal stability, storage stability and reusability. For example, over 83 % of its original activity after 1 h incubation at 70 °C could be remained compared to 18.1 % or 38.4 % remained activity of free HRP and Cyt c, respectively; After 12 cycles of use, over 53 % of original catalytic activity of both the enzymatic reactor could be maintain. The enzymatic kinetic data (Km, Vmax, kcat) and thermodynamic data (Ka, ΔH, ΔS and ΔG) indicated that the improved catalytic performance was attributed to the affinity, selectivity and binding of enzyme to substrate. The two enzymatic reactors could be applied in the fast degradation of 2,4‐dichlorophenol and rifaximin in artificial wastewater, a complete degradation of 2 mg ⋅ mL−1 of 2,4‐dichlorophenol or 20 μg ⋅ mL−1 of rifaximin was achieved in only 20 min.
Electrochemical Ceramic Membrane Reactors in Future Energy and Chemical Process Engineering
Authors: Heddrich Marc P. , Gupta Sanchit , Santhanam Srikanth 
Published: April 2019

AbstractElectrochemical ceramic membrane reactors (ECMRs) with their unique ability to efficiently couple electrical, chemical, and thermal energy sectors can be a large part of the transition toward renewable energies and defossilized economies. ECMRs utilize ceramic conductors to extract or distribute oxide ions or protons – directly controlled by an external electric current. This article gives an overview of ECMR properties along with the functionalities and advantages. For many reactions, e.g., the direct synthesis of ammonia, ECMRs are not available at the preferred temperature range of 300 – 550 °C. To evidence the possibility of such reactors, functional materials are reviewed. A simple thermodynamic methodology is proposed to determine the suitability of reactions for particular combinations of coupled energy sectors.
Consistency and reproducibility of next‐generation sequencing in cytopathology: A second worldwide ring trial study on improved cytological molecular reference specimens
Authors: Pisapia Pasquale , Malapelle Umberto , Roma Gianluca , Saddar Sonika , Zheng Qi , Pepe Francesco , Bruzzese Dario , Vigliar Elena , Bellevicine Claudio , Luthra Rajyalakshmi , Nikiforov Yuri E. , Mayo‐de‐Las‐Casas Clara , Molina‐Vila Miguel Angel , Rosell Rafael , Bihl Michel , Savic Spasenija , Bubendorf Lukas , de Biase Dario , Tallini Giovanni , Hwang David H. , Sholl Lynette M. , Vander Borght Sara , Weynand Birgit , Stieber Daniel , Vielh Philippe , Rappa Alessandra , Barberis Massimo , Fassan Matteo , Rugge Massimo , De Andrea Carlos E. , Lozano Maria D. , Lupi Cristiana , Fontanini Gabriella , Schmitt Fernando , Dumur Catherine I. , Bisig Bettina , Bongiovanni Massimo , Merkelbach‐Bruse Sabine , Büttner Reinhard , Nikiforova Marina N. , Roy‐Chowdhuri Sinchita , Troncone Giancarlo ,   the Molecular Cytopathology Meeting Group
Published: April 2019

BackgroundArtificial genomic reference standards in a cytocentrifuge/cytospin format with well‐annotated genomic data are useful for validating next‐generation sequencing (NGS) on routine cytopreparations. Here, reference standards were optimized to be stained by different laboratories before DNA extraction and to contain a lower number of cells (2 × 105). This was done to better reflect the clinical challenge of working with insufficient cytological material.MethodsA total of 17 worldwide laboratories analyzed customized reference standard slides (slides A‐D). Each laboratory applied its standard workflow. The sample slides were engineered to harbor epidermal growth factor receptor (EGFR) c.2235_2249del15 p.E746_A750delELREA, EGFR c.2369C>T p.T790M, Kirsten rat sarcoma viral oncogene homolog (KRAS) c.38G>A p.G13D, and B‐Raf proto‐oncogene, serine/threonine kinase (BRAF) c.1798_1799GT>AA p.V600K mutations at various allele frequencies (AFs).ResultsEGFR and KRAS mutation detection showed excellent interlaboratory reproducibility, especially on slides A and B (10% and 5% AFs). On slide C (1% AF), either the EGFR mutation or the KRAS mutation was undetected by 10 of the 17 laboratories (58.82%). A reassessment of the raw data in a second‐look analysis highlighted the mutations (n = 10) that had been missed in the first‐look analysis. BRAF c.1798_1799GT>AA p.V600K showed a lower concordance rate for mutation detection and AF quantification.ConclusionsThe data show that the detection of low‐abundance mutations is still clinically challenging and may require a visual inspection of sequencing reads to detect. Genomic reference standards in a cytocentrifuge/cytospin format are a valid tool for regular quality assessment of laboratories performing molecular studies on cytology with low‐AF mutations.
Investigating color appearance in optical see‐through augmented reality
Authors: Hassani Nargess , Murdoch Michael J. 
Published: April 2019

AbstractColor appearance for real objects has been studied over decades and it has been well modeled. However, in augmented reality (AR) environments, virtual content is added to a real background and a mixed appearance is perceived. In this research, we studied color appearance in AR and investigated the applicability of the CAM16 color appearance model, one of the most comprehensive current color appearance models, in an AR environment. Using a benchtop optical mixing apparatus as an AR simulator, objective measurements of mixed colors in AR were performed. Then a psychophysical color matching experiment was performed with combinations of mixed foreground and background colors. The results showed that CAM16 is not accurate in predicting the color appearance in AR environment; therefore, it was modified with the addition of chromatic simultaneous contrast, resulting in an improved fit to the AR experiment data. A second psychophysical color matching experiment was performed on a single display to compare the color perception in AR with color perception in real world from a single display.
Use of Serine/Threonine Ligation for the Total Chemical Synthesis of HMGA1a Protein with Site‐Specific Lysine Acetylations
Authors: Liu Heng , Liu Han , Li Xuechen 
Published: April 2019

AbstractHigh‐mobility‐group (HMG) proteins are a class of abundant non‐histone nuclear proteins, among which HMGA1a is well‐known for its association with transcription regulation as well as tumor formation and disease development. To study the functions of post‐translational modifications, homogeneous HMGA1a protein with site‐specific lysine acetylations (64/66/70/73) has been chemically synthesized. The full‐length HMGA1a protein was assembled through two Ser/Thr ligations of three peptide fragments at Gly37‐Thr37 and Thr75‐Thr76 sites, respectively. Further in vitro studies with chemically synthesized proteins suggested that these acetylations did not significantly affect the CK2‐catalyzed phosphorylation on the HMGA1a acidic tail.
Tandem Systems for Two‐Photon Uncaging of Bioactive Molecules
Authors: Klausen Maxime , Dubois Victor , Verlhac Jean‐Baptiste , Blanchard‐Desce Mireille 
Published: April 2019

AbstractPhotodeprotection of biological ligands with the combination of photolabile protecting groups (PPGs) and two‐photon (2P) excitation techniques offers the possibility to control physiological phenomena with utmost spatial and temporal precision. Yet, designing PPGs with sufficient 2P photosensitivity still remains challenging. Direct enhancement of the 2P absorption properties usually relies on modifications of the PPG structure, which sometimes results in unpredictable and counterproductive effects on the uncaging quantum yield. In that respect, combining known PPGs with a suitable two‐photon light‐harvesting antenna within a modular architecture offers a promising alternative. In this Minireview, we highlight this versatile strategy, with a discussion of different tandem 2P uncagers, relying on photoinduced electron transfer (PET), triplet‐ energy transfer (TT‐ET), or Förster resonance energy transfer (FRET) sensitization to enhance the 2P sensitivity of PPGs.
Unravelling the Aptamer‐Analyte Interaction Dynamics through Fluorescence Quenching in Graphene Quantum Dots (GQDs) Based Homogeneous Assays
Authors: Ang Wei Li , Bonanni Alessandra 
Published: April 2019

AbstractGraphene quantum dots (GQDs) are used here as a biosensing platform for the recognition of the major food contaminant ochratoxin A (OTA), with a fluorescently labelled DNA aptamer (FAM OTA aptamer) functioning as the biorecognition element. The detection principle lies in the formation of noncovalent interactions between the FAM OTA aptamer and the GQD surface, and the consequent fluorescence quenching. The further change in the fluorescence signal, induced by the formation of the FAM OTA Aptamer/OTA conjugate during the detection step, could then be correlated to the presence and concentration of the target analyte. Upon tuning the concentration of GQDs, a switch in the biorecognition mechanism occurred. Specifically, while a lower GQD concentration (0.060 mg/mL) resulted in a restoration of the fluorescence intensity upon incubation with OTA, a higher GQD concentration (0.150 mg/mL) provided a further quenching of the final fluorescence intensity. Upon further calibration study, it was discovered that the latter mechanism provided a better option in terms of linearity of response, detection limit and selectivity.
Tuning the Luminescent Properties of Ruthenium(II) Amino‐1,10‐Phenanthroline Complexes by Varying the Position of the Amino Group on the Heterocycle
Authors: Abel Anton S. , Zenkov Ilya S. , Averin Alexei D. , Cheprakov Andrey V. , Bessmertnykh‐Lemeune Alla G. , Orlinson Boris S. , Beletskaya Irina P. 
Published: April 2019

AbstractEight 1,10‐phenanthrolines bearing one or two 2‐(1‐adamantyloxy)ethylamino substituents attached to different positions of the heterocyclic core were prepared according to SNAr or palladium‐catalyzed amination reactions. Their reaction with cis‐Ru(bpy)2Cl2 (bpy=2,2’‐bipyridine) was investigated and Ru(bpy)2(L)(PF6)2 (phen=1,10‐phenanthroline) (L=amino‐substituted 1,10‐phenanthroline) complexes were obtained in good yields. The electronic structure and emissive properties of these complexes are strongly dependent on the position of the amino substituent in the heterocycle. Emission bands of the complexes bearing 2‐ and 4‐substituted 1,10‐phenanthroline ligands are red‐shifted (up to 56 nm) and less intense compared to that of the parent [Ru(phen)(bpy)2](PF6)2. In contrast, the introduction of the substituent in 3‐ or 5‐position of 1,10‐phenanthroline ring induces only small decrease of luminescence and the brightness of the complex with the 3‐substituted ligand is comparable to that of the parent complex.
Strategic responses of stigmatized Chinese manufacturing firms to formal and informal environmental regulative pressures through enhanced corporate social responsibility effort
Authors: Xu Jia , Wei Jiuchang , (Allan) Chen Haipeng 
Published: April 2019

AbstractLinking two forms of regulative pressures to different motivations for firms' strategic choices, we propose that stigmatized firms' effort to enhance their corporate social responsibility (CSR) reflects legitimacy considerations when faced with formal regulative pressures, but efficiency considerations when faced with informal regulative pressures. We provide empirical support for our theory by investigating publicly listed Chinese manufacturing firms' environmental CSR performance after they are stigmatized for environmental pollution. Our data strongly support our predictions. Stigmatized firms show a lower CSR effort when formal regulative pressure is low and they can easily shun responsibility for polluting the environment, whereas they show a higher CSR effort when informal regulative pressure is high and they cannot easily shun responsibility for the pollution. These findings highlight the unique insight that can be gained by studying stigmatized firms' reactions to different types of regulative pressures as a function of responsibility dilution in an emerging economy.
Naturally processed HLA‐DR3‐restricted HHV‐6B peptides are recognized broadly with polyfunctional and cytotoxic CD4 T‐cell responses
Authors: Becerra‐Artiles Aniuska , Cruz John , Leszyk John D. , Sidney John , Sette Alessandro , Shaffer Scott A. , Stern Lawrence J. 
Published: April 2019

AbstractHuman herpes virus 6B (HHV‐6B) is a widespread virus that infects most people early in infancy and establishes a chronic life‐long infection with periodic reactivation. CD4 T cells have been implicated in control of HHV‐6B, but antigenic targets and functional characteristics of the CD4 T‐cell response are poorly understood. We identified 25 naturally processed MHC‐II peptides, derived from six different HHV‐6B proteins, and showed that they were recognized by CD4 T‐cell responses in HLA‐matched donors. The peptides were identified by mass spectrometry after elution from HLA‐DR molecules isolated from HHV‐6B‐infected T cells. The peptides showed strong binding to matched HLA alleles and elicited recall T‐cell responses in vitro. T‐cell lines expanded in vitro were used for functional characterization of the response. Responding cells were mainly CD3+CD4+, produced IFN‐γ, TNF‐α, and low levels of IL‐2, alone or in combination, highlighting the presence of polyfunctional T cells in the overall response. Many of the responding cells mobilized CD107a, stored granzyme B, and mediated specific killing of peptide‐pulsed target cells. These results highlight a potential role for polyfunctional cytotoxic CD4 T cells in the long‐term control of HHV‐6B infection.
Fluid simulation in a cyclone reverse circulation well washing device based on computational fluid dynamics
Authors: Wang Chun Sheng , Zhang Lei 
Published: April 2019

AbstractThis paper instructs the working principle of the well washing device and its parameter optimization in practical applications. A fluent fluid engineering simulation software was used to establish the calculation model of the bottom flow field. The fluid area inside the well washing device with different orifice diameters at 30, 40, 50, and 60 degrees was simulated, and the gas‐solid two‐phase distribution cloud map and corresponding data were obtained. The results show that the cleaning effect is optimal and with no dead angle when θ = 30° and d = 4 mm. Moreover, the optimization design using simulation technology is reliable and simple. Based on this conclusion, a patent for a cyclone reverse circulation well washing device was invented. Under the same conditions, the above results were also verified by comparison with ordinary washing devices.
Sediment and fluvial particulate carbon flux from an eroding peatland catchment
Authors: Li Changjia , Holden Joseph , Grayson Richard 
Published: April 2019

AbstractErosion and the associated loss of carbon is a major environmental concern in many peatlands and remains difficult to accurately quantify beyond the plot scale. Erosion was measured in an upland blanket peatland catchment (0.017 km2) in northern England using structure‐from‐motion (SfM) photogrammetry, sediment traps and stream sediment sampling at different spatial scales. A net median topographic change of –27 mm yr–1 was recorded by SfM over the 12‐month monitoring period for the entire surveyed area (598 m2). Within the entire surveyed area there were six nested catchments where both SfM and sediment traps were used to measure erosion. Substantial amounts of peat were captured in sediment traps during summer storm events after two months of dry weather where desiccation of the peat surface occurred. The magnitude of topographic change for the six nested catchments determined by SfM (mean value: 5.3 mm, standard deviation: 5.2 mm) was very different to the areal average derived from sediment traps (mean value: –0.3 mm, standard deviation: 0.1 mm). Thus, direct interpolation of peat erosion from local net topographic change into sediment yield at the catchment outlet appears problematic. Peat loss measured at the hillslope scale was not representative of that at the catchment scale. Stream sediment sampling at the outlet of the research catchment (0.017 km2) suggested that the yields of suspended sediment and particulate organic carbon were 926.3 t km–2 yr–1 and 340.9 t km–2 yr–1, respectively, with highest losses occurring during the autumn. Both freeze–thaw during winter and desiccation during long periods of dry weather in spring and summer were identified as important peat weathering processes during the study. Such weathering was a key enabler of subsequent fluvial peat loss from the catchment. © 2019 John Wiley & Sons, Ltd.