bluuebunny/crossref_metadata_2025_split huggingface dataset:
Metabolic reprogramming via mitochondrial delivery for enhanced maturation of chemically induced cardiomyocyte‐like cells
Authors: Nam Yena , Song Yoonji , Seo Seung Ju , Ko Ga Ryang , Lee Seung Hyun , Cha Eunju , Kwak Su Min , Kim Sumin , Shin Mikyung , Jin Yoonhee , Lee Jung Seung 
Published: November 2024

AbstractHeart degenerative diseases pose a significant challenge due to the limited ability of native heart to restore lost cardiomyocytes. Direct cellular reprogramming technology, particularly the use of small molecules, has emerged as a promising solution to prepare functional cardiomyocyte through faster and safer processes without genetic modification. However, current methods of direct reprogramming often exhibit low conversion efficiencies and immature characteristics of the generated cardiomyocytes, limiting their use in regenerative medicine. This study proposes the use of mitochondrial delivery to metabolically reprogram chemically induced cardiomyocyte‐like cells (CiCMs), fostering enhanced maturity and functionality. Our findings show that mitochondria sourced from high‐energy‐demand organs (liver, brain, and heart) can enhance structural maturation and metabolic functions. Notably, heart‐derived mitochondria resulted in CiCMs with a higher oxygen consumption rate capacity, enhanced electrical functionality, and higher sensitivity to hypoxic condition. These results are related to metabolic changes caused by increased number and size of mitochondria and activated mitochondrial fusion after mitochondrial treatment. In conclusion, our study suggests that mitochondrial delivery into CiCMs can be an effective strategy to promote cellular maturation, potentially contributing to the advancement of regenerative medicine and disease modeling.
Defining the features and structure of neutralizing antibody targeting the silent face of the SARS‐CoV‐2 spike N‐terminal domain
Authors: Zhang Zhaoyong , Zhang Yuanyuan , Zhang Yuting , Cheng Linling , Zhang Lu , Yan Qihong , Liu Xuesong , Chen Jiantao , Dai Jun , Guo Yingying , Wei Peilan , Xiong Xinyi , Xiao Juxue , Zhu Airu , Zhuo Jianfen , Cai Ruoxi , Zhang Jingjun , Rao Haiyue , Qu Bin , Zhang Shengnan , Feng Jiaxin , Cheng Jinling , Su Jingyi , Chen Canjie , Li Shu , Zhang Yuanyuan , Chen Lei , Jin Yingkang , Xu Yonghao , Liu Xiaoqing , Li Yimin , Zhao Jingxian , Wang Yanqun , Zhou Qiang , Zhao Jincun 
Published: November 2024

AbstractResearch on virus/receptor interactions has uncovered various mechanisms of antibody‐mediated neutralization against severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2). However, understanding of neutralization by antibodies targeting the silent face, which recognize epitopes on glycan shields, remains limited, and their potential protective efficacy in vivo is not well understood. This study describes a silent face neutralizing antibody, 3711, which targets a non‐supersite on the N‐terminal domain (NTD) of the spike protein. Cryo‐EM structure determination of the 3711 Fab in the spike complex reveals a novel neutralizing epitope shielded by glycans on the spike's silent face. Antibody 3711 inhibits the interaction between the receptor‐binding domain (RBD) and human angiotensin‐converting enzyme 2 (hACE2) through steric hindrance and exhibits superior in vivo protective effects compared to other reported NTD‐targeted monoclonal antibodies (mAbs). Competition assays and antibody repertoire analysis indicate the rarity of antibodies targeting the 3711‐related epitope in SARS‐CoV‐2 convalescents, suggesting the infrequency of NTD silent face‐targeted neutralizing antibodies during SARS‐CoV‐2 infection. As the first NTD silent face‐targeted neutralizing antibody against SARS‐CoV‐2, the identification of mAb 3711, with its novel neutralizing mechanism, enhances our understanding of neutralizing epitopes on glycan shields and elucidates epitope‐guided viral mutations that evade specific antibodies.
Extracellular vesicles in cancers: mechanisms, biomarkers, and therapeutic strategies
Authors: Ma Yuxi , Zhang Xiaohui , Liu Cuiwei , Zhao Yanxia 
Published: November 2024

AbstractExtracellular vesicles (EVs) composed of various biologically active constituents, such as proteins, nucleic acids, lipids, and metabolites, have emerged as a noteworthy mode of intercellular communication. There are several categories of EVs, including exosomes, microvesicles, and apoptotic bodies, which largely differ in their mechanisms of formation and secretion. The amount of evidence indicated that changes in the EV quantity and composition play a role in multiple aspects of cancer development, such as the transfer of oncogenic signals, angiogenesis, metabolism remodeling, and immunosuppressive effects. As EV isolation technology and characteristics recognition improve, EVs are becoming more commonly used in the early diagnosis and evaluation of treatment effectiveness for cancers. Actually, EVs have sparked clinical interest in their potential use as delivery vehicles or vaccines for innovative antitumor techniques. This review will focus on the function of biological molecules contained in EVs linked to cancer progression and their participation in the intricate interrelationship within the tumor microenvironment. Furthermore, the potential efficacy of an EV‐based liquid biopsy and delivery cargo for treatment will be explored. Finally, we explicitly delineate the limitations of EV‐based anticancer therapies and provide an overview of the clinical trials aimed at improving EV development.
Ocular immune‐related diseases: molecular mechanisms and therapy
Authors: Wang Yakun , Gao Shangze , Cao Fan , Yang Hui , Lei Fengyang , Hou Shengping 
Published: November 2024

AbstractOcular immune‐related diseases, represent a spectrum of conditions driven by immune system dysregulation, include but not limit to uveitis, diabetic retinopathy, age‐related macular degeneration, Graves’ ophthalmopathy, etc. The molecular and cellular mechanisms underlying these diseases are typically dysfunctioned immune responses targeting ocular tissues, resulting in inflammation and tissue damage. Recent advances have further elucidated the pivotal role of different immune responses in the development, progression, as well as management of various ocular immune diseases. However, there is currently a relative lack of connection between the cellular mechanisms and treatments of several immune‐related ocular diseases. In this review, we discuss recent findings related to the immunopathogenesis of above‐mentioned diseases. In particular, we summarize the different types of immune cells, inflammatory mediators, and associated signaling pathways that are involved in the pathophysiology of above‐mentioned ophthalmopathies. Furthermore, we also discuss the future directions of utilizing anti‐inflammatory regime in the management of these diseases. This will facilitate a better understanding of the pathogenesis of immune‐related ocular diseases and provide new insights for future treatment approaches.
Programmed cell death: molecular mechanisms, biological functions, diseases, and therapeutic targets
Authors: Qian Shen'er , Long Yao , Tan Guolin , Li Xiaoguang , Xiang Bo , Tao Yongguang , Xie Zuozhong , Zhang Xiaowei 
Published: November 2024

AbstractProgrammed cell death represents a precisely regulated and active cellular demise, governed by a complex network of specific genes and proteins. The identification of multiple forms of programmed cell death has significantly advanced the understanding of its intricate mechanisms, as demonstrated in recent studies. A thorough grasp of these processes is essential across various biological disciplines and in the study of diseases. Nonetheless, despite notable progress, the exploration of the relationship between programmed cell death and disease, as well as its clinical application, are still in a nascent stage. Therefore, further exploration of programmed cell death and the development of corresponding therapeutic methods and strategies holds substantial potential. Our review provides a detailed examination of the primary mechanisms behind apoptosis, autophagy, necroptosis, pyroptosis, and ferroptosis. Following this, the discussion delves into biological functions and diseases associated dysregulated programmed cell death. Finally, we highlight existing and potential therapeutic targets and strategies focused on cancers and neurodegenerative diseases. This review aims to summarize the latest insights on programmed cell death from mechanisms to diseases and provides a more reliable approach for clinical transformation.
Bioinspired micro‐ and nanostructured systems for cancer therapy
Authors: Yang Rui , Zhang Bing , Fei Xiawei , Cong Shanshan , Zhao Shaojie , Zhou Tao , Shen Yanting 
Published: November 2024

AbstractNumerous organisms in nature have demonstrated enhanced biocompatibility, precise tumor targeting capabilities, and efficient tissue traversal within the human body. Drawing inspiration from these organisms, researchers have employed bioengineering, bioconjugation, and micro‐ or nanotechnology to fabricate bioinspired micro‐ and nanostructured systems. These systems play a crucial role in addressing the limitations of conventional anticancer drugs and nanomaterials concerning biocompatibility, effective penetration of physiological barriers, as well as selective tumor targeting, thereby leading to improved therapeutic efficacy while minimizing nonspecific adverse effects on healthy cells. Consequently, extensive exploration of these bioinspired micro‐ and nanostructured systems has been undertaken across various cancer treatment modalities with some progressing into preclinical or clinical stages. However, our understanding of this field remains limited which may impede research progress, clinical translation efforts, and practical applications. Therefore, this study presents a systematic classification of bioinspired micro‐ and nanostructured systems for cancer therapy that comprehensively elucidates their sources of inspiration and design principles. Furthermore, it extensively discusses the current status of clinical translation efforts while identifying prevailing challenges and exploring future prospects. This work will establish a robust theoretical framework and serve as a valuable reference to facilitate advancements in research and clinical application within this field.
Platform or Third‐Party Service Provider? An Analysis of Logistics Mode Selection Strategy for Manufacturers in E‐Commerce Supply Chains
Authors: Chen Ting , Zhang Xiuyi , Jia Fu 
Published: November 2024

ABSTRACTIn recent years, the rise of e‐commerce has led to the dominance of some platforms in the market. Both these platforms and third‐party logistics providers are now offering online logistics services. Under this context, the brand manufacturers face challenges of deciding whether to outsource their logistics needs to third‐party logistics service providers (LSPs), rely on the e‐commerce platform itself, or establish collaborative partnerships with them. The combination between the decision to outsource or cooperate and the choice of service providers yields four typical scenarios: outsourcing to the platform without cooperation (Case PB), outsourcing to the platform with cooperation (Case P), outsourcing to the LSP without cooperation (Case LB), and outsourcing to the LSP with cooperation (Case L). This paper proposes a game‐theoretic model to investigate the manufacturer's optimal selection of logistics mode. We examine the economic and environmental performances of four logistics modes, with a particular emphasis on the mutual benefits and costs shared between manufacturers and their logistics partners. Our results reveal that collaboration in sustainable logistics improvement leads to higher sustainability levels, augmented logistics fees, and increased sales volumes compared to noncollaborative strategies. Additionally, we find that outsourcing to the e‐commerce platform generally results in higher profits and superior environmental performance for manufacturers, contingent upon the sustainability enhancement being sufficiently effective. Empirical evidence from surveys and interviews with industry experts supports our theoretical findings, underscoring the critical role of collaboration in achieving both economic and environmental sustainability within the supply chain. This study contributes to the literature on logistics outsourcing by evaluating manufacturers' strategic decisions in selecting logistics modes and accentuates the importance of cooperation in advancing sustainable logistics practices.
Cortical Functional Connectivity Changes in the Body‐First and Brain‐First Subtypes of Parkinson's Disease
Authors: Conti Matteo , D'Onofrio Valentina , Bovenzi Roberta , Ferrari Valerio , Di Giuliano Francesca , Cerroni Rocco , Pierantozzi Mariangela , Schirinzi Tommaso , Mercuri Nicola Biagio , Antonini Angelo , Guerra Andrea , Stefani Alessandro 
Published: November 2024

AbstractBackgroundRapid eye movement (REM) sleep behavior disorder (RBD) may precede motor symptoms in Parkinson's disease (PD) by years. According to a recent hypothesis, premotor RBD (pRBD) is a marker of the PD body‐first subtype, where synucleinopathy originates from the peripheral autonomic nervous system. Conversely, in the brain‐first subtype, pathology would arise in the brain. Functional connectivity (FC) could provide additional insight into the neurodegenerative process of these putative PD subtypes.ObjectivesWe aim to analyze the possible FC differences between early‐stage PD patients with (PDpRBD+) and without (PDpRBD−) pRBD using high‐density electroencephalography (EEG).MethodsWe enrolled 28 PDpRBD+, 35 PDpRBD−, and 35 healthy controls (HC). Data were recorded with a 64‐channel EEG system, and a source‐reconstruction method was used to identify brain‐region activity. FC was calculated using the weighted phase‐lag index in θ, α, β, and low‐γ bands. Statistical analysis was conducted using network‐based statistic.ResultsWe found a significant trend of decreased α‐FC across PDpRBD+, PDpRBD−, and HC, mainly in prefrontal and temporal areas. The altered α‐FC correlated with Montreal Cognitive Assessment scores in PDpRBD+ and, to a lesser extent, PDpRBD− and with gait/postural disturbances in PDpRBD+ patients only. PDpRBD+ and PDpRBD− had similarly increased FC than HC in a β band network, predominantly involving sensorimotor and limbic areas. The increased β network FC was related to bradykinesia severity in both PD subgroups.ConclusionsCompared to PDpRBD− (brain‐first subtype), PDpRBD+ group (body‐first subtype) demonstrates specific EEG‐FC dysfunctions in the α band, which may reflect early involvement of the cholinergic ascending system. © 2024 International Parkinson and Movement Disorder Society.
Anatomical Landmarks of the Facial Artery and Vein for Intraoral Anastomosis: A Cadaveric Study
Authors: Nakatsuka Kengo , Yano Tomoyuki , Omotehara Takuya , Kawata Shinichi , Itoh Masahiro 
Published: November 2024

ABSTRACTBackgroundIntraoral anastomosis is a widely used technique for microvascular alveolar ridge augmentation and midface reconstruction. However, the predictable anatomical positioning of facial structures, such as the vessels, parotid duct, and facial nerve in the buccal region, has remained unclear. Therefore, we aimed to obtain the anatomical characteristics of these locations to establish surgical landmarks for the intraoral anastomosis of facial vessels.MethodsA total of 26 sides from 13 formaldehyde‐fixed cadavers approximately a month after fixation with a mean age at death of 86.6 ± 11.2 years (range: 55–104 years) were anatomically examined. Facial vessels, nerves, and the parotid duct were dissected intraorally. From the oral cavity side, the X‐axis was defined as the line from the labial commissure to the lowest point of the intertragic notch.ResultsFrom the oral cavity side, all branches of the facial nerve were found under the facial artery and vein. The positioning order along the X‐axis was the facial artery, vein, and parotid duct exit. The facial artery was 21.3 ± 2.2 mm and the facial vein was 39.2 ± 2.7 mm from the labial commissure. Ninety‐two percent of facial veins were found within 15–20 mm of the facial artery on the X‐axis. The parotid duct exit was 46.8 ± 2.0 mm from the labial commissure. In the buccal region, the vessel calibers of the facial artery and vein were 1.8 ± 0.2 and 2.1 ± 0.2 mm, respectively.ConclusionKnowledge of the anatomical relations among the facial artery, vein, parotid duct, and facial nerve from the oral cavity side can enhance the safety and efficacy of midface reconstruction surgeries involving intraoral anastomosis procedures.
Multiband and Wider Bandwidth Plasma Antenna Design Using 3D FDTD
Authors: Avci Selçuk Alparslan , Afacan Erkan 
Published: November 2024

ABSTRACTIn this study, the far‐field characteristics of a plasma dielectric resonator antenna are investigated. The internal structure of the antenna is filled with a plasma material. Surface currents are obtained in an imaginary closed area formed at a certain point outside the plasma antenna. Then, the near field‐far field transformation is performed with the FFT method using the surface currents. The field distributions in the far field of the plasma antenna have been evaluated using three‐dimensional FDTD. The system has shown to work in multiband with wider bandwidths.
Exploring the Perceived Difficulty and Importance of Lower Limb Physical Activities for People With and Without Osteoarthritis: A Discrete Choice Experiment
Authors: Pierobon Andrés , Taylor Will , Siegert Richard , Willink Robin , Bennell Kim , Allen Kelli , Whittaker Jackie , Pearson Jake , Norton Marrissa , Clark Jane , Tay Hilal Ata , Schiphof Dieuwke , Darlow Ben 
Published: November 2024

ABSTRACTBackgroundMany outcome measures used in lower‐limb osteoarthritis (OA) present ceiling effects. This compromises the ability of those measures to accurately assess people with higher levels of physical function. Understanding of the difficulty and importance of physical activities would enable the inclusion of challenging and meaningful activities in new outcome measures.PurposeTo explore the perceived difficulty and importance of 40 physical activities by people with and without lower limb OA.MethodsWe conducted a discrete choice experiment (DCE) using 1000minds software. We recruited people with and without OA using OA databases and social media. Participants were asked to complete two comparison tasks, first about the relative difficulty and then about the importance of the physical activities. Pairwise comparisons were presented (i.e., two alternatives at a time), and participants selected the most difficult/important.ResultsWe analysed data from 613 participants, of whom 215 had OA. Rankings of difficulty and importance were obtained. No major differences existed in the difficulty ranking between people with and without OA. People with OA rated activities like kneeling and balancing activities as more important than those without OA. In contrast, people without OA rated jogging, squatting, and running as more important than those with OA. Challenging activities were generally rated as less important.ConclusionsA DCE ranked 40 different lower limb physical activities in terms of difficulty and importance. Challenging activities were found to be less important than easier ones. People with OA gave more importance to easier activities than people without OA.
Analysis of the parametric configuration impact on BallBot control performance
Authors: Pham Anh‐Duc , Thai Ba Hoa , Dang Phuoc Vinh , Vo Nhu Thanh 
Published: November 2024

AbstractThe BallBot, a versatile robot system, finds applications in various domains of life. It comprises a frame moved by three wheels mounted on a ball. The robot performance is significantly influenced by its parametric configuration, including body mass, chassis size, and ball diameter. This study examines the impact of these configuration parameters on the control of the BallBot. The mathematical model of the BallBot is discussed, considering the assumptions and coordinate systems. To control the robot, a Linear Quadratic Regulator controller is designed. Subsequently, the simulation model is used to assess the effects of changing the initial parametric configuration. It is observed that altering the robot mass has a notable impact on the BallBot response, while changes in the ball diameter have a relatively insignificant effect.
Diabetes Education Program for Nursing Students: A Systematic Review and Meta‐Analysis
Authors: Ahn Jeong‐Ah , Kim Eun‐Mi , Lee Jung Eun , Kim Kyoung‐A 
Published: November 2024

ABSTRACTAimThe purpose of this study was to summarise the current state of the science on diabetes mellitus education programs for nursing students.DesignA systematic review and meta‐analysis.MethodsEligible studies were identified by searching PubMed, EMBASE, CINAHL, and Cochrane Library databases. Randomised controlled trials and quasi‐experimental studies, published in English between 2013 and 2022, that examined diabetes education programs for nursing students were considered in the review. The quality of the articles was evaluated using the Joanna Briggs Institute's Critical Appraisal Checklist. Key information such as authors, study focus, population, sample size, details of intervention and control group treatments, outcome variables, and main findings were extracted and summarised in a data extraction form for further analyses and syntheses.ResultsThe literature search identified 464 articles, from which 13 studies were evaluated in the systematic review. Most studies (n = 12, 92.3%) used technology‐based teaching methods, such as high‐fidelity simulations, mobile applications, and virtual reality simulations. Regarding the evaluation of diabetes education program effectiveness, the majority of studies showed significant improvements in knowledge (n = 8, 61.5%), followed by satisfaction with learning (n = 4, 30.8%), nursing skill performance (n = 3, 23.1%), and self‐confidence (n = 3, 23.1%) in nursing students. In meta‐analyses, technology‐based teaching interventions, compared to traditional education, showed no statistically significant improvement in diabetes knowledge (standard mean difference 9.52, 95% CI [−0.18, 19.21], p = 0.05) and self‐efficacy (standard mean difference 24.09, 95% CI [−10.75, 58.92], p = 0.18). Despite this, technology‐based methods demonstrated favourable effects on knowledge and self‐efficacy against traditional education. Findings highlight the importance of emerging technology‐based diabetes education programs tailored for nursing students, crucial for enhancing positive educational outcomes.No Patient or Public Contribution.