bluuebunny/crossref_metadata_2025_split huggingface dataset:
Theoretical Model and Experimental Research on Friction and Torque Characteristics of Hydro-viscous Drive in Mixed Friction Stage
Authors: Cui Hongwei , Wang Qiliang , Lian Zisheng , Li Long 
Published: October 2019

AbstractThe hydro-viscous drive (HVD) has been widely used in fan transmission in vehicles, fans, and scraper conveyors for step-less speed regulation or soft starting. In the mixed friction stage, the contact, friction, and torque characteristics of friction pairs are very complex and change at any time. The characteristics of the frictional and hydrodynamic lubrication states were studied in order to calculate and predict the friction and torque characteristics of the friction pairs in the mixed friction stage. The fluid torque was calculated by applying the average shear stress model and the load-carrying capacity of asperity was determined on the basis of the fractal contact theory. In addition, the contact friction coefficient of the friction pairs was taken into consideration and measured by using the MM1000-III friction and wear testing machine. The asperity friction torque and total torque in the mixed friction stage were obtained and finally, the test rig for the torque characteristics was set up. The results show that the contribution to the total torque is shared by the oil film and the asperity friction. The friction coefficient decreases sharply at first and then increases with a change in the relative rotational speed, following the Stribeck curve closely, and the contact frictional coefficient slowly decreases with increase in the pressure between the friction pairs. The torque between the friction pairs is provided by the asperity friction, and the torque due to the oil film reduces to zero. When the thickness of the oil film is small, a major contribution to the total torque is due to the asperity friction. The total torque also increases with the decrease in the film thickness ratio. Therefore, by theoretical analysis and experimental verification, the torque of the friction pairs in the mixed friction stage can be accurately calculated using the average shear stress model and asperity friction torque model.
PGM5 is a promising biomarker and may predict the prognosis of colorectal cancer patients
Authors: Sun Yifan , Long Haihua , Sun Lin , Sun Xiujuan , Pang Liping , Chen Jianlin , Yi Qingqun , Liang Tianwei , Shen Yongqi 
Published: October 2019

AbstractBackgroundPhosphoglucomutase (PGM), a key enzyme in the metabolism of glucose-1-phosphate and glucose-6-phosphate, has been found to be associated with proliferation, invasion, and metastasis of cancer. However, the expression and function of PGM5 in colorectal cancer (CRC) remains unknown.MethodsWe tested PGM5 mRNA and protein expression levels in 79 CRC tissue and their matched adjacent tissue samples by qRT-PCR and immunohistochemistry, respectively. Overall survival (OS) was estimated with the Kaplan–Meier method and compared between groups with the log-rank test. We performed multivariable Cox regression analyses to identify factors associated with CRC risk. The cell proliferation, migration and invasion abilities of CRC cells were detected by using CCK-8, Transwell migration and invasion assays, respectively.ResultsThe PGM5 protein levels expression in CRC tissues were significantly lower than those in the adjacent tissues (t = 5.035,P < 0.001), and Kaplan–Meier analysis indicated that low PGM5 expression were significantly associated with poor overall survival (P = 0.0069). Univariate and multivariate analyses demonstrated that PGM5 was an independent risk factor for overall survival (hazard ratio = 0.3951,P = 0.014). PGM5 overexpression significantly inhibited the proliferation, invasion and migration abilities of CRC cells. On the contrary, knockdown of PGM5 promotes the invasion and migration of CRC cells.ConclusionsPMG5 regulates proliferation, invasion, and migration in the CRC and decreased PGM5 is associated with poor prognosis. Therefore, PGM5 is a promising biomarker in CRC and decreased PGM5 may predict poor overall survival in patients with CRC.
Development of the Australian Cancer Atlas: spatial modelling, visualisation, and reporting of estimates
Authors: Duncan Earl W. , Cramb Susanna M. , Aitken Joanne F. , Mengersen Kerrie L. , Baade Peter D. 
Published: October 2019

AbstractBackgroundIt is well known that the burden caused by cancer can vary geographically, which may relate to differences in health, economics or lifestyle. However, to date, there was no comprehensive picture of how the cancer burden, measured by cancer incidence and survival, varied by small geographical area across Australia.MethodsThe Atlas consists of 2148 Statistical Areas level 2 across Australia defined by the Australian Statistical Geography Standard which provide the best compromise between small population and small area. Cancer burden was estimated for males, females, and persons separately, with 50 unique sex-specific (males, females, all persons) cancer types analysed. Incidence and relative survival were modelled with Bayesian spatial models using the Leroux prior which was carefully selected to provide adequate spatial smoothing while reflecting genuine geographic variation. Markov Chain Monte Carlo estimation was used because it facilitates quantifying the uncertainty of the posterior estimates numerically and visually.ResultsThe results of the statistical model and visualisation development were published through the release of the Australian Cancer Atlas (https://atlas.cancer.org.au) in September, 2018. The Australian Cancer Atlas provides the first freely available, digital, interactive picture of cancer incidence and survival at the small geographical level across Australia with a focus on incorporating uncertainty, while also providing the tools necessary for accurate estimation and appropriate interpretation and decision making.ConclusionsThe success of the Atlas will be measured by how widely it is used by key stakeholders to guide research and inform decision making. It is hoped that the Atlas and the methodology behind it motivates new research opportunities that lead to improvements in our understanding of the geographical patterns of cancer burden, possible causes or risk factors, and the reasons for differences in variation between cancer types, both within Australia and globally. Future versions of the Atlas are planned to include new data sources to include indicators such as cancer screening and treatment, and extensions to the statistical methods to incorporate changes in geographical patterns over time.
Circulating DNA as prognostic biomarker in patients with advanced hepatocellular carcinoma: a translational exploratory study from the SORAMIC trial
Authors: Alunni-Fabbroni Marianna , Rönsch Kerstin , Huber Thomas , Cyran Clemens C. , Seidensticker Max , Mayerle Julia , Pech Maciej , Basu Bristi , Verslype Chris , Benckert Julia , Malfertheiner Peter , Ricke Jens 
Published: October 2019

AbstractBackgroundLiquid biopsy based on cell-free DNA circulating in plasma has shown solid results as a non-invasive biomarker. In the present study we evaluated the utility of circulating free DNA (cfDNA) and the sub-type tumor DNA (ctDNA) in hepatocellular cancer (HCC) patients to assess therapy response and clinical outcome.MethodsA cohort of 13 patients recruited in the context of the SORAMIC trial with unresectable, advanced HCC and different etiological and clinicopathological characteristics was included in this exploratory study. Plasma samples were collected between liver micro-intervention and beginning of sorafenib-based systemic therapy and then in correspondence of three additional follow-ups. DNA was isolated from plasma and next generation sequencing (NGS) was performed on a panel of 597 selected cancer-relevant genes.ResultscfDNA levels showed a significant correlation with the presence of metastases and survival. In addition cfDNA kinetic over time revealed a trend with the clinical history of the patients, supporting its use as a biomarker to monitor therapy. NGS-based analysis on ctDNA identified 28 variants, detectable in different combinations at the different time points. Among the variants, HNF1A, BAX and CYP2B6 genes showed the highest mutation frequency and a significant association with the patients’ clinicopathological characteristics, suggesting a possible role as driver genes in this specific clinical setting.ConclusionsTaken together, the results support the prognostic value of cfDNA/ctDNA in advanced HCC patients with the potential to predict therapy response. These findings support the clinical utility of liquid biopsy in advanced HCC improving individualized therapy and possible earlier identification of treatment responders.
Two novel UPLC methods utilizing two different analytical columns and different detection approaches for the simultaneous analysis of velpatasvir and sofosbuvir: application to their co-formulated tablet
Authors: Moustapha Moustapha Eid , El-Gamal Rania Mohamed , Belal Fathalla Fathalla 
Published: October 2019

Abstract
In the present study two different RSLC columns, Acclaim RSLC 120 C18, 5.0 µm, 4.6 × 150 mm (column A) and Acclaim RSLC 120 C18, 2.2 µm, 2.1 × 100 mm (Column B) were utilized for the analysis of velpatasvir (VPS) in presence of sofosbuvir (SFV), where due to the encountered fluorescent properties of VPS fluorescent detection at 405 nm after excitation at 340 nm (Method 1) was used for its detection where the non-fluorescent SFV did not interfere. The same columns were further utilized for the simultaneous determination of SFV and VPS either in bulk form or in their combined tablet, where UV- spectrophotometric detection at 260 nm was selected for the simultaneous analysis of both drugs (Method 2). A mobile phase consisting of NaH2PO4, pH 2.5 (with phosphoric acid) and acetonitrile in a ratio of 60:40 v/v was used for both methods. The mobile phase was pumped at a flow rate of 1.0 mL/min when using column, A and 0.5 mL/min when using column B. The methods showed good linearity over the concentration ranges of 1.0–5.0 and 2.5–10.0 ng/mL for VPS when utilizing Method 1 A and B respectively. Where the linearity concentration range was from 30.0–150.0 to 120–600.0 ng/mL for VPS and SFV respectively when applying Method 2. Both methods 1 and 2 were performed by utilizing the two analytical columns. The different chromatographic parameters as retention time, resolution, number of theoretical plates (N), capacity factor, tailing factor and selectivity were carefully optimized. The results show that comparing the performance of the two utilized columns revealed that shorter column (2.1 mm × 100 mm) with small particle packing was superior to the longer column (4.6 × 150 mm) for the analysis of the studied drugs allowing a reduction of the analysis time by 70% without any detrimental effect on performance. This prompts the decrease of the investigation costs by saving money on organic solvents and expanding the overall number of analyses per day.
Fusion transcription factors for strong, constitutive expression of cellulases and xylanases in Trichoderma reesei
Authors: Derntl Christian , Mach Robert L. , Mach-Aigner Astrid R. 
Published: October 2019

Abstract

Background
The filamentous ascomycete T. reesei is industrially used to produce cellulases and xylanases. Cost-effective production of cellulases is a bottleneck for biofuel production. Previously, different strain and process optimizations were deployed to enhance enzyme production rates. One approach is the overexpression of the main activator Xyr1 and a second is the construction of synthetic transcription factors. Notably, these genetic manipulations were introduced into strains bearing the wild-type xyr1 gene and locus.

Results
Here, we constructed a Xyr1-deficient strain expressing a non-functional truncated version of Xyr1. This strain was successfully used as platform strain for overexpression of Xyr1, which enhanced the cellulase and xylanase production rates under inducing conditions, with the exception of lactose—there the cellulase production was severely reduced. Further, we introduced fusion transcription factors consisting of the DNA-binding domain of Xyr1 and the transactivation domain of either Ypr1 or Ypr2 (regulators of the sorbicillinoid biosynthesis gene cluster). The fusion of Xyr1 and Ypr2 yielded a moderately transactivating transcription factor, whereas the fusion of Xyr1 and Ypr1 yielded a highly transactivating transcription factor that induced xylanases and cellulases nearly carbon source independently. Especially, high production levels of xylanases were achieved on glycerol.

Conclusion
During this study, we constructed a Xyr1-deficient strain that can be fully reconstituted, which makes it an ideal platform strain for Xyr1-related studies. The mere overexpression of Xyr1 turned out not to be a successful strategy for overall enhancement of the enzyme production rates. We gained new insights into the regulatory properties of transcription factors by constructing respective fusion proteins. The Xyr1–Ypr1-fusion transcription factor could induce xylanase production rates on glycerol to outstanding extents, and hence could be deployed in the future to utilize crude glycerol, the main co-product of the biodiesel production process.
Expression of microRNA in human retinal pigment epithelial cells following infection with Zaire ebolavirus
Authors: Oliver Genevieve F. , Orang Ayla V. , Appukuttan Binoy , Marri Shashikanth , Michael Michael Z. , Marsh Glenn A. , Smith Justine R. 
Published: October 2019

Abstract

Objective
Survivors of Ebola virus disease (EVD) are at risk of developing blinding intraocular inflammation—or uveitis—which is associated with retinal pigment epithelial (RPE) scarring and persistence of live Zaire ebolavirus (EBOV) within the eye. As part of a large research project aimed at defining the human RPE cell response to being infected with EBOV, this work focused on the microRNAs (miRNAs) associated with the infection.

Results
Using RNA-sequencing, we detected 13 highly induced and 2 highly repressed human miRNAs in human ARPE-19 RPE cells infected with EBOV, including hsa-miR-1307-5p, hsa-miR-29b-3p and hsa-miR-33a-5p (up-regulated), and hsa-miR-3074-3p and hsa-miR-27b-5p (down-regulated). EBOV-miR-1-5p was also found in infected RPE cells. Through computational identification of putative miRNA targets, we predicted a broad range of regulatory activities, including effects on innate and adaptive immune responses, cellular metabolism, cell cycle progression, apoptosis and autophagy. The most highly-connected molecule in the miR-target network was leucine-rich repeat kinase 2, which is involved in neuroinflammation and lysosomal processing. Our findings should stimulate new studies on the impact of miRNA changes in EBOV-infected RPE cells to further understanding of intraocular viral persistence and the pathogenesis of uveitis in EVD survivors.
DYAMOND: the DYnamics of the Atmospheric general circulation Modeled On Non-hydrostatic Domains
Authors: Stevens Bjorn , Satoh Masaki , Auger Ludovic , Biercamp Joachim , Bretherton Christopher S. , Chen Xi , Düben Peter , Judt Falko , Khairoutdinov Marat , Klocke Daniel , Kodama Chihiro , Kornblueh Luis , Lin Shian-Jiann , Neumann Philipp , Putman William M. , Röber Niklas , Shibuya Ryosuke , Vanniere Benoit , Vidale Pier Luigi , Wedi Nils , Zhou Linjiong 
Published: October 2019

Abstract
A review of the experimental protocol and motivation for DYAMOND, the first intercomparison project of global storm-resolving models, is presented. Nine models submitted simulation output for a 40-day (1 August–10 September 2016) intercomparison period. Eight of these employed a tiling of the sphere that was uniformly less than 5 km. By resolving the transient dynamics of convective storms in the tropics, global storm-resolving models remove the need to parameterize tropical deep convection, providing a fundamentally more sound representation of the climate system and a more natural link to commensurately high-resolution data from satellite-borne sensors. The models and some basic characteristics of their output are described in more detail, as is the availability and planned use of this output for future scientific study. Tropically and zonally averaged energy budgets, precipitable water distributions, and precipitation from the model ensemble are evaluated, as is their representation of tropical cyclones and the predictability of column water vapor, the latter being important for tropical weather.
Automatic conversion of OSM data into LULC maps: comparing FOSS4G based approaches towards an enhanced performance
Authors: Patriarca J. , Fonte C. C. , Estima J. , de Almeida J.-P. , Cardoso A. 
Published: October 2019

AbstractOSM2LULC is a software package developed to automatically convert OpenStreetMap (OSM) data into Land Use Land Cover (LULC) maps using Free and Open Source Software for Geospatial (FOSS4G) tools. It needs to be highly efficient given the increasing detail of OSM data and the need to apply it to large extent regions. In this article, a comparison between the implementation of OSM2LULC in different available GIS platforms is made using both vector and raster data structures, which resulted in different versions. A description of the differences of each version is made and, to assess their performance, they were applied to four different study areas with different characteristics, in terms of available OSM data and area size. The performance of each version was evaluated taking into account: the overall processing time required to obtain LULC maps; and differences in the results obtained when different data structures (vector and raster) were used. Results showed that the adoption of a strategy that favors interoperability between FOSS4G and the combined use of both vector and raster data promotes a performance increase. After analysing the topological relationships of OSM data, the conversion to raster data format and the execution of procedural parts with such data indicated significant performance gains, without any positional distortions that significantly compromise the applicability of the final result in further case scenarios.
General principles and escalation options of immunotherapy in autoantibody-associated disorders of the CNS
Authors: Ayzenberg Ilya , Faissner Simon , Tomaske Laura , Richter Daniel , Behrendt Volker , Gold Ralf 
Published: October 2019

Abstract
Autoimmune diseases associated with antineuronal and antiglial autoantibodies (Abs) is one of the most rapidly expanding research fields in clinical neuroimmunology, with more than 30 autoantibodies described so far. Being associated with a wide range of clinical presentations these syndromes can be diagnostically challenging. Surface or intracellular antigen localizations are crucial for the treatment response and outcome. In the latter Abs are mostly of paraneoplastic cause and tumor management should be performed as soon as possible in order to stop peripheral antigen stimulation. Immunotherapy should be started early in both groups, before irreversible neuronal loss occurs. Despite serious prognosis, aggressive therapeutic approaches can be effective in many cases. In this article we review main pathogenic mechanisms leading to Abs-related syndromes and describe standard as well as emerging strategies of immunotherapy, including tocilizumab and bortezomib. Several special therapeutic approaches will be illustrated by clinical cases recently treated in our department.
Role of Patient Maximizing-Minimizing Preferences in Thyroid Cancer Surveillance
Authors: Evron Joshua M. , Reyes-Gastelum David , Banerjee Mousumi , Scherer Laura D. , Wallner Lauren P. , Hamilton Ann S. , Ward Kevin C. , Hawley Sarah T. , Zikmund-Fisher Brian J. , Haymart Megan R. 
Published: October 2019

PURPOSE To understand the effect of patient preferences on thyroid cancer surveillance intensity. PATIENTS AND METHODS Eligible patients diagnosed with thyroid cancer between January 1, 2014, and December 31, 2015, from the Georgia and Los Angeles County SEER registries were surveyed between February 2017 and October 2018 (N = 2,632; response rate, 63%). Patient reports on health care utilization in the past year and responses to the validated Medical Maximizer-Minimizer Scale were linked to SEER data in the 2,183 disease-free patients. Ordered logistic regression was performed using a cumulative logit with nonproportional odds. RESULTS Of disease-free patients, 31.6% were classified as minimizers, 42.5% as moderate maximizers, and 25.9% as strong maximizers. In the past year, 25.2%, 27.3%, and 38.5% of minimizers, moderate maximizers, and strong maximizers, respectively, had ≥ 4 doctor visits, and 18.3%, 24.9%, and 29.5%, respectively, had ≥ 2 neck ultrasounds. When controlling for age, sex, race and ethnicity, comorbidity, stage, and SEER site, strong maximizers (compared with minimizers) were significantly more likely to report ≥ 4 doctor visits (odds ratio [OR], 1.45; 95% CI, 1.10-1.92), ≥ 2 neck ultrasounds (OR, 1.58; 95% CI, 1.17-2.14), ≥ 1 radioactive iodine scan (OR, 1.73; 95% CI, 1.19-2.50), and ≥ 1 additional imaging study (OR, 2.06; 95% CI, 1.56-2.72). CONCLUSION Among patients with thyroid cancer who have been declared disease free, preference for a more maximal versus minimal approach to medical care is associated with increased number of physician visits and imaging tests. Because increased surveillance does not clearly correlate with improved outcomes, poses potential risks to patients, and contributes to increased healthcare costs, stronger consideration of the role of patient preferences is necessary when framing discussions on surveillance.
The Nuclear Receptor COUP-TFII Regulates Amhr2 Gene Transcription via a GC-Rich Promoter Element in Mouse Leydig Cells
Authors: Mehanovic Samir , Mendoza-Villarroel Raifish E , Viger Robert S , Tremblay Jacques J 
Published: October 2019

AbstractThe nuclear receptor chicken ovalbumin upstream promoter–transcription factor type II (COUP-TFII)/NR2F2 is expressed in adult Leydig cells, and conditional deletion of the Coup-tfii/Nr2f2 gene impedes their differentiation. Steroid production is also reduced in COUP-TFII–depleted Leydig cells, supporting an additional role in steroidogenesis for this transcription factor. COUP-TFII action in Leydig cells remains to be fully characterized. In the present work, we report that COUP-TFII is an essential regulator of the gene encoding the anti-Müllerian hormone receptor type 2 (Amhr2), which participates in Leydig cell differentiation and steroidogenesis. We found that Amhr2 mRNA levels are reduced in COUP-TFII–depleted MA-10 Leydig cells. Consistent with this, COUP-TFII directly activates a −1486 bp fragment of the mouse Amhr2 promoter in transient transfection assays. The COUP-TFII responsive region was localized between −67 and −34 bp. Chromatin immunoprecipitation assay confirmed COUP-TFII recruitment to the proximal Amhr2 promoter whereas DNA precipitation assay revealed that COUP-TFII associates with the −67/−34 bp region in vitro. Even though the −67/−34 bp region contains an imperfect nuclear receptor element, COUP-TFII–mediated activation of the Amhr2 promoter requires a GC-rich sequence at −39 bp known to bind the specificity protein (SP)1 transcription factor. COUP-TFII transcriptionally cooperates with SP1 on the Amhr2 promoter. Mutations that altered the GCGGGGCGG sequence at −39 bp abolished COUP-TFII–mediated activation, COUP-TFII/SP1 cooperation, and reduced COUP-TFII binding to the proximal Amhr2 promoter. Our data provide a better understanding of the mechanism of COUP-TFII action in Leydig cells through the identification and regulation of the Amhr2 promoter as a novel target.