bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dynamin binding protein is required forXenopus laeviskidney development
Authors: DeLay Bridget D. , Baldwin Tanya A. , Miller Rachel K. 
Published: September 2018

ABSTRACTThe adult human kidney contains over one million nephrons, with each nephron consisting of a tube containing segments that have specialized functions in nutrient and water absorption and waste excretion. The embryonic kidney ofXenopus laevisconsists of a single functional nephron composed of regions that are analogous to those found in the human nephron, making it a simple model for the study of nephrogenesis. The exocyst complex, which traffics proteins to the cell membrane in vesicles via CDC42, is essential for normal kidney development. Here, we show that the CDC42-GEF, dynamin binding protein (Dnmbp/Tuba), is essential for nephrogenesis inXenopus.dnmbpis expressed inXenopusembryo kidneys during development, and knockdown of Dnmbp using two separate morpholino antisense oligonucleotides results in reduced expression of late pronephric markers, whereas the expression of early markers of nephrogenesis remains unchanged. A greater reduction in expression of markers of differentiated distal and connecting tubules was seen in comparison to proximal tubule markers, indicating that Dnmbp reduction may have a greater impact on distal and connecting tubule differentiation.dnmbpknockout using CRISPR results in a similar reduction of late markers of pronephric tubulogenesis. Overexpression ofdnmbpin the kidney also resulted in disrupted pronephric tubules, suggesting thatdnmbplevels in the developing kidney are tightly regulated, with either increased or decreased levels leading to developmental defects. Together, these data suggest that Dnmbp is required for nephrogenesis.
Integrated Analysis Revealed Hub Genes in Breast Cancer
Authors: Jin Haoxuan , Huang Xiaoyan , Shao Kang , Li Guibo , Wang Jian , Yang Huanming , Hou Yong 
Published: September 2018

AbstractThe aim of this study was to identify the hub genes in breast cancer and provide further insight into the tumorigenesis and development of breast cancer. To explore the hub genes in breast cancer, we performed an integrated bioinformatics analysis. Two gene expression profiles were downloaded from the GEO database. The differentially expressed genes (DEGs) were identified by using the “limma” package. Then, we performed Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis to explore the functional annotation and potential pathways of the DEGs. Next, protein–protein interaction (PPI) network analysis and weighted gene coexpression network analysis (WGCNA) were conducted to screen for hub genes. To confirm the reliability of the identified hub genes, we obtained TCGA-BRCA data by using WGCNA to screen for genes that were strongly related to breast cancer. By combining the results from the GEO and TCGA datasets, we finally identified 15 real hub genes in breast cancer. Finally, we performed an overall survival analysis to explore the connection between the expression of hub genes and the overall survival time of breast cancer patients. We found that for all hub genes, higher expression was associated with significantly shorter overall survival times among breast cancer patients.
A reverse Turing-test for predicting social deficits in people with Autism
Authors: d’Arc Baudouin Forgeot , Devaine Marie , Daunizeau Jean 
Published: September 2018

Abstract:Social symptoms of autism spectrum disorder (ASD) are typically viewed as consequences of an impaired Theory of Mind, i.e. the ability to understand others’ covert mental states. Here, we test the assumption that such “mind blindness” may be due to the inability to exploit contextual knowledge about, e.g., the stakes of social interactions, to make sense of otherwise ambiguous cues (e.g., idiosyncratic responses to social competition). In this view, social cognition in ASD may simply reduce to non-social cognition, i.e. cognition that is not informed by the social context. We compared 24 adult participants with ASD to 24 neurotypic participants in a repeated dyadic competitive game against artificial agents with calibrated mentalizing sophistication. Critically, participants were framed to believe that they were competing against humans (social framing) or not (non-social framing), hence the “reverse Turing test”. In contrast to control participants, the strategy of people with ASD is insensitive to the game’s framing, i.e. they do not constrain their understanding of others’ behaviour with the contextual knowledge about the game (cf. competitive social framing). They also outperform controls when playing against simple agents, but are outperformed by them against recursive algorithms framed as human opponents. Moreover, computational analyses of trial-by-trial choice sequences in the game show that individuals with ASD rely on a distinctive cognitive strategy with subnormal flexibility and mentalizing sophistication. These computational phenotypes yield 79% diagnosis classification accuracy and explain 62% of the severity of social symptoms in people with ASD.
Fast fluorescence lifetime imaging reveals the aggregation processes of α-synuclein and polyglutamine in aging Caenorhabditis elegans
Authors: Laine Romain F. , Sinnige Tessa , Ma Kai Yu , Haack Amanda J. , Poudel Chetan , Gaida Peter , Curry Nathan , Perni Michele , Nollen Ellen A.A. , Dobson Christopher M. , Vendruscolo Michele , Kaminski Schierle Gabriele S. , Kaminski Clemens F. 
Published: September 2018

AbstractThe nematode worm Caenorhabditis elegans has emerged as an important model organism to study the molecular mechanisms of protein misfolding diseases associated with amyloid formation because of its small size, ease of genetic manipulation and optical transparency. Obtaining a reliable and quantitative read-out of protein aggregation in this system, however, remains a challenge. To address this problem, we here present a fast time-gated fluorescence lifetime imaging (TG-FLIM) method and show that it provides functional insights into the process of protein aggregation in living animals by enabling the rapid characterisation of different types of aggregates. More specifically, in longitudinal studies of C. elegans models of Parkinson’s and Huntington’s diseases, we observed marked differences in the aggregation kinetics and the nature of the protein inclusions formed by α-synuclein and polyglutamine. In particular, we found that α-synuclein inclusions do not display amyloid-like features until late in the life of the worms, whereas polyglutamine forms amyloid characteristics rapidly in early adulthood. Furthermore, we show that the TG-FLIM method is capable of imaging live and non-anaesthetised worms moving in specially designed agarose micro-chambers. Taken together, our results show that the TG-FLIM method enables high-throughput functional imaging of living C. elegans that can be used to study in vivo mechanisms of aggregation and that has the potential to aid the search for therapeutic modifiers of protein aggregation and toxicity.
Complexity Matching and Requisite Variety
Authors: Mahmoodi Korosh , West Bruce J. , Grigolini Paolo 
Published: September 2018

AbstractComplexity matching emphasizes the condition necessary to efficiently transport information from one complex system to another and the mechanism can be traced back to the 1957Introduction to Cyberneticsby Ross Ashby. Unlike this earlier work we argue that complexity can be expressed in terms of crucial events, which are generated by the processes of spontaneous self-organization. Complex processes, ranging from biological to sociological, must satisfy the homeodynamic condition and host crucial events that have recently been shown to drive the information transport between complex systems. We adopt a phenomenological approach, based on the subordination to periodicity that makes it possible to combine homeodynamics and self-organization induced crucial events. The complexity of crucial events is defined by the waiting-time probability density function (PDF) of the intervals between consecutive crucial events, which have an inverse power law (IPL) PDFψ(τ) ∝1/(τ)μwith 1 <μ< 3. We establish the coupling between two temporally complex systems using a phenomenological approach inspired by models of swarm cognition and prove that complexity matching, namely sharing the same IPL indexμ, facilitates the transport of information, generating perfect synchronization, reminiscent of, but distinct from chaos synchronization. This advanced form of complexity matching is expected to contribute a significant progress in understanding and improving the bio-feedback therapies.Author SummaryThis paper is devoted to the control of complex dynamical systems, inspired to real processes of biological and sociological interest. The concept of complexity we adopt focuses on the assumption that the processes of self-organization generate intermittent fluctuations and that the time distance between two consecutive fluctuations is described by a distribution density with an inverse power law structure making the second moment of these time distances diverge. These fluctuations are called crucial events and are responsible for the ergodicity breaking that is widely revealed by the experimental observation of biological dynamics. We argue that the information transport from one to another complex system is ruled by these crucial events and we propose an efficient theoretical prescription leading to qualitative agreement with experimental results, shedding light into the processes of social learning. The theory of this paper is expected to have important medical applications, such as an improvement of the biofeedback techniques, the heart-brain communication and a significant progress on cognition and the contribution of emotions to cognition.
Minority community willingness to pay for earthquake insurance
Authors: Zhang Chun-Min , Qian Zhen-Wei 
Published: September 2018

Purpose
The purpose of this paper is to investigate the relationship between potential affecting factors and the local communities’ willingness to pay (WTP) for housing earthquake insurance (HEI) in the context of ethnic minority communities.

Design/methodology/approach
A literature review was done to identify possible factors affecting WTP for HEI. Fieldwork was conducted in 2017 in Dali Minority Autonomous Prefecture, where the first Chinese HEI was launched in 2015. Interviews were done in two earthquake-prone counties, as the main ethnic minority communities in the area. A total of 536 questionnaires were collected and used as empirical data for testing the impacts mechanism.

Findings
Respondents’ risk perception, risk exposure, self-prevention behaviors, government aid, insurance experience and sociodemographic characteristics were hypothesized as theoretical indicators correlated to WTP for HEI. Empirical analysis results predict that WTP for HEI is significantly influenced by risk perception, insurance experience, government aid, and age and out-migrating labors. It is evident that higher risk perception and more insurance experience lead to stronger desire for HEI coverage. However, dependency on government aid negatively affects WTP for HEI. Moreover, WTP for HEI is negative in relation to age and out-migrating labors. Surprisingly, ethnic-culture factors were not statistically significant to WTP for HEI.

Originality/value
This paper is an attempt to identify and verify factors affecting WTP for HEI, bridging the gap of inadequate research on WTP for HEI in ethnic minority communities.
Drawing the case studies together: synthesis of case studies and group discussions
Authors: Gibson Terry David , Tongwa Aka Festus , Bari Sarwar , Chantry Guillaume , Gupta Manu , Molina Jesusa Grace , Shresha Nisha , Norton John , Parajuli Bhubaneswari , Rahmawati Hepi , Aretaake Ruiti 
Published: September 2018

Purpose
The purpose of this paper is to individually examine the findings from eight case studies presented in this special issue and comparatively identify the findings regarding local learning and action.

Design/methodology/approach
Underlying research questions regarding power and powerlessness in regard to addressing underlying risk factors affecting local populations form the basis for the discussion. Proceedings of a collaborative workshop conducted with the contributing authors are analysed qualitatively to identify learning relating to the research questions emerging from the case studies individually and collectively.

Findings
A number of strategies and tactics for addressing underlying risk factors affecting local populations were identified from the case studies, including collaboration and cohesion. Campaigning, lobbying, communications and social mobilisation in an attempt to bridge the gap between local concerns and the decision-making of government and other powerful actors. Innovation and local mobilisation to address shortcomings in government support for disaster reduction and development. Communications as a first base to influence behaviour of both communities and government. Social change through empowerment of women to act in disaster reduction and development.

Research limitations/implications
The outcomes of the action research conducted by the authors individually and collectively highlight the necessity for bridging different scales of action through a range of strategies and tactics to move beyond local self-reliance to influence on underlying risk factors. The action research process employed may have wider applications in gathering and formalising local-level experience and knowledge.

Practical implications
The case studies and their analysis present a range of practical strategies and tactics to strengthen local resilience and address underlying risk factors which are replicable in other contexts.

Originality/value
Practitioners are activists and do not often engage in critical reflection and analysis. The method presented here offers a means of achieving this in order to generate learning from local-level experience. The findings contribute to the consideration of cross-scale action to address underlying risk factors which impact local communities.
Relationships between supply performance of pharmacies in Chile and their quality of customer service
Authors: Rojas Fernando , Coluccio Giulani , Vega Juan 
Published: September 2018

Purpose
This paper aims to describe relationships between constructs adapted to the pharmaceutical field with respect to the measurement of the supply chains strategic and operational performance in private pharmacies, relating it to the quality of service perceived by users.

Design/methodology/approach
Relationships among the indicated constructs were validated through the use of structural equation models. This study includes the use of questionnaires adapted and applied to suppliers (pharmaceutical chemists and supply staff), operational managers (pharmacy assistants) and users (patients and clients) who visit different segments of pharmacies, such as chain businesses and independents located in the region of Valparaíso, Chile. The sample collected information from 128 establishments and 601 people, through which different relationships between segments were compared.

Findings
A structural model was validated on the basis of statistical principles. Furthermore, positive relationships were observed in the constructs studied where pharmacy chains had a statistical significance of less than 5 per cent between the responsibility of the supply process and the experience of the users, whereas independent pharmacies had a very significant causal relationship between operational managers and users.

Practical implications
The proposal can help predict the quality of service perceived by the user based on the behavior of sales and supply staff, which may be similar in other markets with similar characteristics.

Originality/value
The structural model proposed is original and adapts measurement scales validated from previous studies to be able to apply them in the pharmaceutical retail market.
Understanding the career development and employability of information technology students
Authors: McKenzie Sophie , Coldwell-Neilson Jo , Palmer Stuart 
Published: September 2018

Purpose
The purpose of this paper is to understand the career development and employability needs of undergraduate information technology (IT) students at an Australian University, and their relation to students’ career interest. While many factors and stakeholders contribute to student career development, this study focused specifically on the student experience. Social cognitive career theory (SCCT) is used as an approach to understand the students’ needs of career development and employability.

Design/methodology/approach
An online survey was completed by 126 IT students to record information about students’ career development and employability background and needs.

Findings
The results demonstrate that SCCT helps understand the factors that impact on IT students’ career development, with their outcome expectations and self-efficacy informed by prior studies in IT and their need for access to “IT professionals” to contribute towards their career interest. In addition, IT students rely on academic achievement and experiential learning, rather than career resources, to guide their career development and employability.

Research limitations/implications
The data collected in this study are limited to one discipline (IT) at one university, which necessarily limits the generalisability of the specific results.

Practical implications
Career development is a complex, life-stage-dependant and discipline-specific process that varies for every decision maker. This research makes an important contribution in presenting the IT student experience and demonstrates how an appropriate career development model can help understand students’ needs. This outcome will help educators better support IT students to build the career interest.

Originality/value
This study explored the often-overlooked student experience of career development, providing valuable insight into IT students’ needs.
Affect vs cognition as antecedents of selection behaviour of elective courses using fsQCA
Authors: Jain Vishal , Jain Parul 
Published: September 2018

Purpose
The purpose of this paper is to investigate students’ attitude based on affective, behavioural and cognitive components. It will ascertain whether there is a link between the three components of attitude, which leads the possible classification of the elective courses.

Design/methodology/approach
The current study considers the students of the International Business Administration Department from Rustaq College of Applied Sciences, Ministry of Higher Education, Sultanate of Oman, during the academic year 2016–2017. The list of the elective courses was obtained from the existing study plan. A total of 101 students assessed elective courses’ affective and cognitive learning with the use of a web-based survey instrument.

Findings
An empirical analysis of the selection criterion was performed employing fuzzy set qualitative comparative analysis. The results of this study found that students rated 17 elective courses into 8 different configurations (triodes) based on various degrees assigned to attitudinal variables.

Research limitations/implications
The present study explores the interaction between affective and cognitive factors in determining the selection behaviour of students. It is an investigation into the context of student choices regarding elective courses, especially the decision to select or not to select available courses.

Originality/value
The world of feelings and beliefs is always open to learning and self-development for the students. Students are continuously involved in taking charge of high-stakes decisions; one of them is the selection of elective courses. However, the critical components into the overall evaluations of their selection behaviour, such as feelings and beliefs, are not well studied.
Customer orientation or employee orientation: which matters more? The moderating role of firm size
Authors: Park Cheol , Jun Jongkun , Lee Thaemin , Lee Heejung 
Published: September 2018

PurposeThis study aims to examine several antecedents of employee satisfaction (ES) and turnover intention (TI), including customer orientation (CO) and employee orientation (EO). The purposes are to investigate the effect of EO and CO and their interaction on employee performance, and to verify the moderating effect of firm size.Design/methodology/approachA mixed model with firm size as a potential moderator was constructed through a hierarchical linear modeling approach with data collected from 1,006 employees at 127 firms.FindingsThe results indicate that customer and EO and their interaction affected ES, CO and its interaction with EO significantly affected TI and the effects differed according to firm size. These results suggest that the influence of customer and EO depends on firm size.Originality/valueThis study contributes to verifying the effect of EO and CO and the interaction effects on employee performance, an area that has remained unexamined in the literature. It also investigates the moderating effect of firm size on EO and CO, which affects employee performance. It is suggested that companies determine whether EO or CO matters more according to the size of company.
“Shopping for Items” or “Partnering for Performance”? A framework of purchasing practices for value co-creation in post-outsourcing buyer–supplier relationships
Authors: Makkonen Hannu , Nordberg-Davies Sini , Olkkonen Rami 
Published: September 2018

Purpose
The article aims to further understanding of purchasing practices in post-outsourcing buyer–supplier relationships, and it provides a holistic approach and onceptualizations with which to balance the perspectives of purchasing as an intra-firm operational function and as a strategic activity embedded in relationship and network management.

Design/methodology/approach
The research is based on an inductive-oriented case study. The data comprised 61 thematic interviews conducted at 17 buyer companies (electricity distribution companies), 11 service supplier companies and six third-party organizations. The case features a setting in which a growing number of electricity distribution companies have outsourced operational functions regarding network construction, maintenance and fault repair and purchased these services from service suppliers representing a developing service market.

Findings
The study explicates the role of purchasing as an element with the most impact on post-outsourcing buyer–supplier relationship outcomes and development. The performance of the buyer–supplier relationship either accelerates or inhibits the new service supplier’s entry to the field and motivates or demotivates the electricity distribution companies to outsource their activities. This mechanism links to the respective development of supplier markets and buyer industry transformation.

Originality/value
The focal study provides a holistic approach and conceptualizations with which to balance the perspectives of purchasing as an intra-firm operational function and as a strategic activity embedded in relationship and network management. In particular, the study provides conceptual development on purchasing practices in post-outsourcing relationships and concrete managerial implications for dealing with such circumstances. The focal study includes case notes that facilitate using the study for teaching purposes.