bluuebunny/crossref_metadata_2025_split huggingface dataset:
Resveratrol Targets Urokinase-Type Plasminogen Activator Receptor Expression to Overcome Cetuximab-Resistance in Oral Squamous Cell Carcinoma
Authors: Uzawa Katsuhiro , Amelio Antonio L. , Kasamatsu Atsushi , Saito Tomoaki , Kita Akihiro , Fukamachi Megumi , Sawai Yuki , Toeda Yuriko , Eizuka Keitaro , Hayashi Fumihiko , Kato-Kase Ikuko , Sunohara Masataka , Iyoda Manabu , Koike Kazuyuki , Nakashima Dai , Ogawara Katsunori , Endo-Sakamoto Yosuke , Shiiba Masashi , Takiguchi Yuichi , Yamauchi Mitsuo , Tanzawa Hideki 
Published: August 2019

AbstractDrug resistance to anti-cancer agents is a major concern regarding the successful treatment of malignant tumors. Recent studies have suggested that acquired resistance to anti-epidermal growth factor receptor (EGFR) therapies such as cetuximab are in part caused by genetic alterations in patients with oral squamous cell carcinoma (OSCC). However, the molecular mechanisms employed by other complementary pathways that govern resistance remain unclear. In the current study, we performed gene expression profiling combined with extensive molecular validation to explore alternative mechanisms driving cetuximab-resistance in OSCC cells. Among the genes identified, we discovered that a urokinase-type plasminogen activator receptor (uPAR)/integrin β1/Src/FAK signal circuit converges to regulate ERK1/2 phosphorylation and this pathway drives cetuximab-resistance in the absence of EGFR overexpression or acquired EGFR activating mutations. Notably, the polyphenolic phytoalexin resveratrol, inhibited uPAR expression and consequently the signaling molecules ERK1/2 downstream of EGFR thus revealing additive effects on promoting OSCC cetuximab-sensitivity in vitro and in vivo. The current findings indicate that uPAR expression plays a critical role in acquired cetuximab resistance of OSCC and that combination therapy with resveratrol may provide an attractive means for treating these patients.
Impact of ICM disturbances on the mean pressure profile of galaxy clusters: A prospective study of the NIKA2 SZ large program with MUSIC synthetic clusters
Authors: Ruppin F. , Sembolini F. , De Petris M. , Adam R. , Cialone G. , Macías-Pérez J. F. , Mayet F. , Perotto L. , Yepes G. 
Published: August 2019

Context. The mean pressure profile of the galaxy cluster population plays an essential role in cosmological analyses. An accurate characterization of the shape, intrinsic scatter, and redshift evolution of this profile is necessary to estimate some of the biases and systematic effects that currently prevent cosmological analyses based on thermal Sunyaev-Zel’dovich (tSZ) surveys from obtaining precise and unbiased cosmological constraints. This is one of the main goals of the ongoing NIKA2 SZ large program, which aims at mapping the tSZ signal of a representative cluster sample selected from thePlanckand ACT catalogs and spans a redshift range 0.5 <  z <  0.9.Aims. To estimate the impact of intracluster medium (ICM) disturbances that can be detected by NIKA2 on the mean pressure profile of galaxy clusters, we realized a study based on a synthetic cluster sample that is similar to that of the NIKA2 SZ large program.Methods. To reach this goal we employed the hydrodynamical N-body simulation Marenostrum MUltidark SImulations of galaxy Clusters (MUSIC). We simulated realistic NIKA2 andPlancktSZ observations, which were jointly analyzed to estimate the ICM pressure profile of each cluster. A comparison of the deprojected profiles with the true radial profiles directly extracted from the MUSIC simulation allowed us to validate the NIKA2 tSZ pipeline and to study the impact of ICM disturbances on the characterization of the ICM pressure distribution even at high redshift. After normalizing each profile by the integrated quantities estimated under the hydrostatic equilibrium hypothesis, we evaluated the mean pressure profile of the twin sample and show that it is compatible with that extracted directly from the MUSIC simulation in the scale range that can be recovered by NIKA2. We studied the impact of cluster dynamical state on both its shape and associated scatter.Results. We observe that atR500the scatter of the distribution of normalized pressure profiles associated with the selected morphologically disturbed clusters is 65% larger than that associated with relaxed clusters. Furthermore, we show that using a basic modeling of the thermal pressure distribution in the deprojection procedure induces a significant increase of the scatter associated with the mean normalized pressure profile compared to the true distribution extracted directly from the simulation.Conclusions. We conclude that the NIKA2 SZ large program will facilitate characterization of the potential redshift evolution of the mean pressure profile properties due to the performance of the NIKA2 camera, thereby allowing for a precise measurement of cluster morphology and ICM thermodynamic properties up toR500at high redshift.
Kontrastmittelsonografie bei einem seltenen bilateralen
renalen Karzinom mit sekundärer Entzündung und Nekrose bei einer
Katze
Authors: Fromme Vivian , Köhler Claudia , Langguth Johann , Morales Isabel Cabrera , Alef Michaele , Kiefer Ingmar 
Published: August 2019

ZusammenfassungEin 17 Jahre alter, kastrierter Europäisch-Kurzhaar-Kater wurde aufgrund von
Inappetenz vorgestellt. Die klinische Untersuchung war ohne besonderen Befund.
Bei der sonografischen Untersuchung des Abdomens konnten an beiden Nieren
überwiegend reflexarme Umfangsvermehrungen im Rinden- und Kapselbereich
dargestellt werden. Die Ausprägung der Nierenveränderungen ließ sich durch
Kontrastmittelsonografie (contrast enhanced ultrasound, CEUS) und Kontrast-CT
eindrücklich darstellen. Von den Umfangsvermehrungen wurden in Narkose unter
Ultraschallkontrolle Feinnadelaspirate gewonnen, wobei teilweise eine in der
Kleintiermedizin unübliche Aspiration von dorsal erfolgte. Die entnommenen
Proben waren diagnostisch; die zytologische Untersuchung ergab beidseits den
deutlichen Verdacht auf ein renales Karzinom mit sekundärer Entzündung und
Nekrose. Unter symptomatischer Therapie besserte sich der Zustand der Katze
zunächst, doch wurde das Tier 2 Wochen später euthanasiert.
Bilateraler Reflux – pro intravesikale Techniken
Authors: Kranz Jennifer , Steffens Joachim 
Published: August 2019

ZusammenfassungAlle Antirefluxplastiken zeichnen sich durch eine hohe Erfolgsrate von über 90 % aus. Gemeinsames Ziel aller Verfahren ist die Bildung eines submukösen Tunnels, der viermal länger ist als der Ureterdurchmesser. Weltweit haben sich bei einem nicht dilatierten Harnleiter die extravesikale Antirefluxplastik nach Lich-Gregoir und beim dilatierten Ureter die Psoas Hitch-Ureterozystoneostomie bewährt. Zu den intravesikalen Operationsverfahren zählen die Technik nach Politano-Leadbetter und die gekreuzt-trigonale Reimplantation nach Cohen. Diese offen oder laparoskopisch/robotisch durchgeführten Standardverfahren weisen gegenüber einer endoskopischen Unterspritzung des refluxiven Harnleiterostiums höhere Erfolgsraten auf.Indikation zu einer operativen Refluxkorrektur sind der höhergradige Refluxgrad IV und V, zusätzliche Begleitanomalien (Hutch-Divertikel, Ureter duplex, ektope Harnleitermündung) und eine erfolglose endoskopische Ostiumunterspritzung 1. Zudem ist eine ausreichende Nierenfunktion von ≥ 15 % der refluxiven Niere wichtig. Bei eingeschränkter Partialfunktion kann kein Nierenfunktionsgewinn erwartet werden. Im postoperativen Verlauf ergibt sich bei einer hochgradig eingeschränkten Nierenfunktion meist ein weiterer Funktionsverlust.
Analysis of Panax ginseng miRNAs and Their Target Prediction Based on High-Throughput Sequencing
Authors: Wang Yingfang , Peng Mengyuan , Chen Yanlin , Wang Wenjuan , He Zhihua , Yang Zemin , Lin Zhiyun , Gong Mengjuan , Yin Yongqin , Zeng Yu 
Published: August 2019

Abstract
Panax ginseng has been widely and effectively used as medicine for thousands of years. However, only limited studies have been conducted to date on ginseng miRNAs. In the present study, we collected 3 ginseng samples from the Changbai Mountain in China. Small RNA libraries were constructed and sequenced on the Illumina HiSeq platform. Sequencing analyses identified 3798 miRNAs, including 298 known miRNAs and 3500 potentially novel miRNAs. The miR166, miR159, and miR396 families were among the most highly expressed miRNAs in all libraries. The results of miRNA expression analyses were validated by qRT-PCR. Target gene prediction through computational and pathway annotation analyses revealed that the primary pathways were related to plant development, including metabolic processes and single-organism processes. It has been reported that plant miRNAs might be one of the hidden bioactive ingredients in medicinal plants. Based on the combined use of RNAhybrid, Miranda, and TargetScan software, a total of 50,992 potential human genes were predicted as the putative targets of 2868 miRNAs. Interestingly, the enriched KEGG pathways were associated with some human diseases, especially cancer, immune system diseases, and neurological disorders, and this could support the clinical use of ginseng. However, the human targets of ginseng miRNAs should be confirmed by further experimental validation. Our results provided valuable insight into ginseng miRNAs and the putative roles of these miRNAs.
A Clean Cut: Minimally Invasive Mitral Valve Replacement after Heart Transplantation
Authors: Kremer Jamila , Al-Maisary Sameer Saeed Ahmed , Szabó Gábor 
Published: August 2019

A 50-year-old female presented with progressive heart failure due to obstruction of the mitral valve after heart transplantation in 2008. Through the occlusion catheter (IntraClude Intra-Aortic Occlusion Device), aortic cross-clamping, antegrade cardioplegia, and aortic root venting were performed.Our case reports the first published study on minimally invasive mitral valve replacement for valve stenosis through intraluminal aortic clamping. The hazard of traumatic injuries can be reduced by avoiding resternotomy and circumventing adhesions between the aorta and the pulmonary artery after heart transplantation. Minimally invasive valve surgery is a safe and effective method with regard to short- and long-term results, especially in redo operations.
Laparoscopic Distal Pancreatectomy of a Solid Pseudopapillary Tumor (SPT) Achieves Long-Term Oncologic Safety and Multiorgan Preservation
Authors: ElHaddad Ahmed , Gasparella Paolo , Castellani Christoph , Singer Georg , Sorantin Erich , Zach Klara , Till Holger 
Published: August 2019

AbstractThe oncological safety of a laparoscopic approach for solid pseudopapillary tumors (SPTs) of the pancreas remains a matter of debate. We present the long-term follow-up of an adolescent girl with an SPT in the pancreatic tail. A multimodality workup including magnetic resonance imaging (MRI) revealed a complex, spherical mass of 4.4 cm × 3.6 cm × 4 cm most likely located in the pancreatic tail. All routine laboratory investigations and tumor markers were within normal limits (alpha fetoprotein [AFP], cancer antigen 125 [CA125], CA 19–9, carcinoembryonic antigen [CEA], adrenocorticotropic hormone [ACTH]). Diagnostic laparoscopy was performed to verify the origin of the tumor in the pancreatic tail. In a three-port technique the tumor was mobilized of the splenic vessels until a distal pancreatectomy could be completed. Histopathological examination confirmed the complete resection of a low-grade malignant SPT. The postoperative course was unremarkable. Regular pediatric oncological follow-up examinations for 3 years, including MRI every 6 months, ruled out recurrence and confirmed preservation of splenic and pancreatic functions. While data about the technical feasibility of a laparoscopic approach to pancreatic SPT are already available, this pediatric case report adds a long-term oncological and functional success to the available literature.
A semiclassical theory of phase-space dynamics of interacting bosons
Authors: Mathew R , Tiesinga E 
Published: August 2019

Abstract
We study the phase-space representation of dynamics of bosons in the semiclassical regime where the occupation number of the modes is large. To this end, we employ the van Vleck-Gutzwiller propagator to obtain an approximation for the Green’s function of the Wigner distribution. The semiclassical analysis incorporates interference of classical paths and reduces to the truncated Wigner approximation (TWA) when the interference is ignored. Furthermore, we identify the Ehrenfest time after which the TWA fails. As a case study, we consider a single-mode quantum nonlinear oscillator, which displays collapse and revival of observables. We analytically show that the interference of classical paths leads to revivals, an effect that is not reproduced by the TWA or a perturbative analysis.
Collective oscillation modes of a superfluid Bose–Fermi mixture
Authors: Wen Wen , Wang Ying , Wang Jianyong 
Published: August 2019

Abstract
In this work, we present a theoretical study for the collective oscillation modes, i.e. quadrupole, radial and axial mode, of a mixture of Bose and Fermi superfluids in the crossover from a Bardeen-Cooper-Schrieffer (BCS) superfluid to a molecular Bose–Einstein condensate (BEC) in harmonic trapping potentials with cylindrical symmetry of experimental interest. To this end, we start from the coupled superfluid hydrodynamic equations for the dynamics of Bose–Fermi superfluid mixtures and use the scaling theory that has been developed for a coupled system. The collective oscillation modes of Bose–Fermi superfluid mixtures are found to crucially depend on the overlap integrals of the spatial derivations of density profiles of the Bose and Fermi superfluids at equilibrium. We not only present the explicit expressions for the overlap density integrals, as well as the frequencies of the collective modes provided that the effective Bose–Fermi coupling is weak, but also test the valid regimes of the analytical approximations by numerical calculations in realistic experimental conditions. In the presence of a repulsive Bose–Fermi interaction, we find that the frequencies of the three collective modes of the Bose and Fermi superfluids are all upshifted, and the change speeds of the frequency shifts in the BCS–BEC crossover can characterize the different groundstate phases of the Bose–Fermi superfluid mixtures for different trap geometries.
Effect of microstructures and textures on the anisotropy of mechanical properties of AZ31 magnesium alloy sheets subjected to high strain rate rolling
Authors: Liu Xiao , Wang Yangyang , Zhu Biwu , Xie Chao , Sun Youping , Yang Hui 
Published: August 2019

Abstract
The AZ31 magnesium alloy sheets with different microstructures and textures were produced by high strain rate rolling (HSRR) at various temperatures and strain rates. Tensile tests were carried out on AZ31 magnesium alloy sheets along rolling direction (RD), transverse direction (TD) and 45° along the rolling direction (45° RD) at room temperature. The microstructures and textures were determined. The Schmid factors (SFs) contour lines were also calculated. The tilt angle of the c-axis towards the RD affects the anisotropy of tensile strength. For the AZ31 magnesium alloy sheets subjected to relatively low strain rate rolling, the anisotropy of yield strength is comparatively obvious, due to the elongated grains. When the loading direction is close to basal plane, prismatic slip is not favored during tension deformation at room temperature. The anisotropy of yield strength mainly depends on the critical resolved shear stress/Schmid factor (CRSS/m) ratios of second-order (2nd-order) pyramidal slip.
Accurate estimation of microbial sequence diversity with Distanced
Authors: Hackmann Timothy J 
Published: August 2019

AbstractMotivationMicrobes are the most diverse organisms on the planet. Deep sequencing of ribosomal DNA (rDNA) suggests thousands of different microbes may be present in a single sample. However, errors in sequencing have made any estimate of within-sample (alpha) diversity uncertain.ResultsWe developed a tool to estimate alpha diversity of rDNA sequences from microbes (and other sequences). Our tool, Distanced, calculates how different (distant) sequences would be without sequencing errors. It does this using a Bayesian approach. Using this approach, Distanced accurately estimated alpha diversity of rDNA sequences from bacteria and fungi. It had lower root mean square prediction error (RMSPE) than when using no tool (leaving sequencing errors uncorrected). It was also accurate with non-microbial sequences (antibody mRNA). State-of-the-art tools (DADA2 and Deblur) were far less accurate. They often had higher RMSPE than when using no tool. Distanced thus represents an improvement over existing tools. Distanced will be useful to several disciplines, given microbial diversity affects everything from human health to ecosystem function.Availability and implementationDistanced is freely available at https://github.com/thackmann/Distanced.Supplementary informationSupplementary data are available at Bioinformatics online.
Physiological consequences of rising water salinity for a declining freshwater turtle
Authors: Agha Mickey , Yanagitsuru Yuzo R , Fangue Nann A , Nowakowski A Justin , Kojima Laura V , Cech Joseph J , Riley Melissa K , Freeman Janna , Cocherell Dennis E , Todd Brian D 
Published: August 2019

AbstractSea-level rise, drought and water diversion can all lead to rapid salinization of freshwater habitats, especially in coastal areas. Increased water salinities can in turn alter the geographic distribution and ecology of freshwater species including turtles. The physiological consequences of salinization for freshwater turtles, however, are poorly known. Here, we compared the osmoregulatory response of two geographically separate populations of the freshwater Western Pond Turtle (Actinemys marmorata)—a species declining across its range in western North America—to three constant salinities: 0.4 ppt, 10 ppt and 15 ppt over 2 weeks. We found that turtles from a coastal estuarine marsh population regulated their plasma osmolality at lower levels than their conspecifics from an inland freshwater creek population 45 km away. Plasma osmolalities were consistently lower in estuarine marsh turtles than the freshwater creek turtles over the entire 2-week exposure to 10 ppt and 15 ppt water. Furthermore, estuarine marsh turtles maintained plasma osmolalities within 1 SD of their mean field osmolalities over the 2-week exposure, whereas freshwater creek turtles exceeded their field values within the first few days after exposure to elevated salinities. However, individuals from both populations exhibited body mass loss in 15 ppt water, with significantly greater loss in estuarine turtles. We speculate that the greater ability to osmoregulate by the estuarine marsh turtles may be explained by their reduced feeding and drinking in elevated salinities that was not exhibited by the freshwater creek population. However, due to mass loss in both populations, physiological and behavioural responses exhibited by estuarine marsh turtles may only be effective adaptations for short-term exposures to elevated salinities, such as those from tides and when traversing saline habitats, and are unlikely to be effective for long-term exposure to elevated salinity as is expected under sea-level rise.
A new unbiased and highly automated approach to find new prognostic markers in preclinical research
Authors: Neidnicht Martin , Mittermüller Daniela , Effenberger-Neidnicht Katharina 
Published: August 2019

Abstract
Data acquisition in (pre)clinical studies is often based on a hypothesis. Numerical algorithms, however, may help to find biomarkers from existing data without formulating any hypothesis. By simply assessing whether a statistical relationship exists between two parameters from a (unlimited) database, every (in)conceivable combination of data becomes a hypothesis. The aim was to create an unbiased and highly automated approach for secondary analysis of (pre)clinical research, including the possibility of a non-linear functional relationship. In our example, an almost homogeneous database was formed by overall 45 parameters (vital, blood and plasma parameters) measured in 11 individual experimental studies at 6 different time points using 57 rats without and 63 rats with systemic inflammation following lipopolysaccharide infusion. For each rat, four group classifiers (treatment, survival, study, ID) were used to get valid samples by a later filtering of the statistical base. Any information about the hypothesis leading to the respective studies was suppressed. In order to assess whether a statistical relationship exists, a total of six different functional prototypes (linear and non-linear) were postulated and examined for their regression. Regression quality, correlation and significance were obtained in form of matrices. In our example, ultimately 510 300 regressions were optimized, automatically evaluated and filtered. The developed algorithm is able to reveal statistical relationships from a nearly crude database with low effort by systematic and unbiased analysis. The finding of well-known correlations proves its reliability, whose validity could be increased by clean aggregation of different studies. In addition, new interesting hints for future research could be gained. Thus, unknown markers can be found which are associated with an increased risk of death during systemic inflammation and sepsis. A further development of the program is planned including multiple regressions (more than two parameters could be related to each other) or cluster analysis.
Non-invasive assessment of ventriculo-arterial coupling using aortic wave intensity analysis combining central blood pressure and phase-contrast cardiovascular magnetic resonance
Authors: Bhuva Anish N , D’Silva A , Torlasco C , Nadarajan N , Jones S , Boubertakh R , Van Zalen J , Scully P , Knott K , Benedetti G , Augusto J B , Bastiaenen Rachel , Lloyd G , Sharma S , Moon J C , Parker K H , Manisty C H , Hughes Alun D 
Published: August 2019

Abstract

Background
Wave intensity analysis (WIA) in the aorta offers important clinical and mechanistic insight into ventriculo-arterial coupling, but is difficult to measure non-invasively. We performed WIA by combining standard cardiovascular magnetic resonance (CMR) flow-velocity and non-invasive central blood pressure (cBP) waveforms.

Methods and results
Two hundred and six healthy volunteers (age range 21–73 years, 47% male) underwent sequential phase contrast CMR (Siemens Aera 1.5 T, 1.97 × 1.77 mm2, 9.2 ms temporal resolution) and supra-systolic oscillometric cBP measurement (200 Hz). Velocity (U) and central pressure (P) waveforms were aligned using the waveform foot, and local wave speed was calculated both from the PU-loop (c) and the sum of squares method (cSS). These were compared with CMR transit time derived aortic arch pulse wave velocity (PWVtt). Associations were examined using multivariable regression. The peak intensity of the initial compression wave, backward compression wave, and forward decompression wave were 69.5 ± 28, −6.6 ± 4.2, and 6.2 ± 2.5 × 104 W/m2/cycle2, respectively; reflection index was 0.10 ± 0.06. PWVtt correlated with c or cSS (r = 0.60 and 0.68, respectively, P &lt; 0.01 for both). Increasing age decade and female sex were independently associated with decreased forward compression wave (−8.6 and −20.7 W/m2/cycle2, respectively, P &lt; 0.01) and greater wave reflection index (0.02 and 0.03, respectively, P &lt; 0.001).

Conclusion
This novel non-invasive technique permits straightforward measurement of wave intensity at scale. Local wave speed showed good agreement with PWVtt, and correlation was stronger using the cSS than the PU-loop. Ageing and female sex were associated with poorer ventriculo-arterial coupling in healthy individuals.