bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reversal of homocysteine-induced neurotoxicity in rat hippocampal neurons by astaxanthin: evidences for mitochondrial dysfunction and signaling crosstalk
Authors: Wang Xian-jun , Chen Wang , Fu Xiao-ting , Ma Jin-kui , Wang Mei-hong , Hou Ya-jun , Tian Da-chen , Fu Xiao-yan , Fan Cun-dong 
Published: October 2018

AbstractElevated plasma level of homocysteine (Hcy) represents an independent risk for neurological diseases, and induction of oxidative damage is considered as one of the most important pathomechanisms. Astaxanthin (ATX) exhibits strong antioxidant activity in kinds of experimental models. However, the potential of ATX against Hcy-induced neurotoxicity has not been well explored yet. Herein, the neuroprotective effect of ATX against Hcy-induced neurotoxicity in rat hippocampal neurons was examined, and the underlying mechanism was evaluated. The results showed that ATX pre-treatment completely reversed Hcy-induced neurotoxicity through inhibiting cell apoptosis in rat primary hippocampal neurons. The mechanical investigation revealed that ATX effectively blocked Hcy-induced mitochondrial dysfunction by regulating Bcl-2 family and opening of mitochondrial permeability transition pore (MPTP). ATX pre-treatment also attenuated Hcy-induced oxidative damage via inhibiting the release of intracellular reactive oxide species (ROS) and superoxide anion through regulating MPTP opening. Moreover, normalization of MAPKs and PI3K/AKT pathways also contributed to ATX-mediated protective effects. Taken together, these results above suggested that ATX has the potential to reverse Hcy-induced neurotoxicity and apoptosis by inhibiting mitochondrial dysfunction, ROS-mediated oxidative damage and regulation of MAKPs and AKT pathways, which validated the strategy of using ATX could be a highly effective way in combating Hcy-mediated neurological disorders.
Rapid diagnostic assay for detection of cellulose in urine as biomarker for biofilm-related urinary tract infections
Authors: Antypas Haris , Choong Ferdinand X. , Libberton Ben , Brauner Annelie , Richter-Dahlfors Agneta 
Published: October 2018

AbstractThe ability of uropathogenic Escherichia coli (UPEC) to adopt a biofilm lifestyle in the urinary tract is suggested as one cause of recurrent urinary tract infections (UTIs). A clinical role of UPEC biofilm is further supported by the presence of bacterial aggregates in urine of UTI patients. Yet, no diagnostics exist to differentiate between the planktonic and biofilm lifestyle of bacteria. Here, we developed a rapid diagnostic assay for biofilm-related UTI, based on the detection of cellulose in urine. Cellulose, a component of biofilm extracellular matrix, is detected by a luminescent-conjugated oligothiophene, which emits a conformation-dependent fluorescence spectrum when bound to a target molecule. We first defined the cellulose-specific spectral signature in the extracellular matrix of UPEC biofilm colonies, and used these settings to detect cellulose in urine. To translate this optotracing assay for clinical use, we composed a workflow that enabled rapid isolation of urine sediment and screening for the presence of UPEC-derived cellulose in <45 min. Using multivariate analysis, we analyzed spectral information obtained between 464 and 508 nm by optotracing of urine from 182 UTI patients and 8 healthy volunteers. Cellulose was detected in 14.8% of UTI urine samples. Using cellulose as a biomarker for biofilm-related UTI, our data provide direct evidence that UPEC forms biofilm in the urinary tract. Clinical implementation of this rapid, non-invasive and user-friendly optotracing diagnostic assay will potentially aid clinicians in the design of effective antibiotic treatment.
Whole genome sequencing, variant analysis, phylogenetics, and deep sequencing of Zika virus strains
Authors: Shrivastava Susmita , Puri Vinita , Dilley Kari A. , Ngouajio Erica , Shifflett Jessica , Oldfield Lauren M. , Fedorova Nadia B. , Hu Lihui , Williams Torrey , Durbin Alan , Amedeo Paolo , Rashid Sujatha , Shabman Reed S. , Pickett Brett E. 
Published: October 2018

AbstractThe recent emergence of Zika virus (ZIKV) has been concentrated in the Caribbean, Southeastern United States, and South- and Central America; resulting in travel-based cases being reported around the globe. As multi-disciplinary collaborations are combatting the ZIKV outbreak, the need to validate the sequence of existing strains has become apparent. Here, we report high-quality sequence data for multiple ZIKV strains made publicly available through the National Institutes of Health- (NIH) funded biorepository, BEI Resources (www.beiresources.org). Next-generation sequencing, 3′ rapid amplification of cDNA ends (RACE), and viral genome annotation pipelines generated GenBank sequence records for 16 BEI Resources strains. Minor variants, consensus mutations, and consensus insertions/deletions were identified within the viral stocks using next-generation sequencing (NGS) and consensus changes were confirmed with Sanger sequencing. Bioinformatics analyses of the sequencing results confirm that the virus stocks available to the scientific research community through BEI Resources adequately represent the viral population diversity of ZIKV.
Realization of real-time X-ray stereoscopic vision during interventional procedures
Authors: Deng Kai , Wei Bo , Chen Mo , Huang Zhiyin , Wu Hao 
Published: October 2018

AbstractDuring interventional procedures, the deficiencies of nonstereoscopic vision increase the difficulty of identifying the anteroposterior direction and pathways of vessels. Therefore, achieving real-time stereoscopic vision during interventional procedures is meaningful. Pairs of X-ray images were captured with identical parameter settings, except for different rotation angles (represented as the α angle). The resulting images at these α angles were used as left-eye and right-eye views and were horizontally merged into single left-right 3D images. Virtual reality (VR) glasses were used for achieving stereo vision. Pairs of X-ray images from four angiographies with different α angles (1.8–3.4°) were merged into left-right 3D images. Observation with VR glasses can produce realistic stereo views of vascular anatomical structure. The results showed that the optimal α angles accepted by the brain for generating stereo vision were within a narrow range (approximately 1.4–4.1°). Subsequent tests showed that during transcatheter arterial chemoembolization, 3D X-ray stereoscopic images provided significantly improved spatial discrimination and convenience for identifying the supply vessels of a liver tumor and its anteroposterior direction compared with plain X-ray images (all P < 0.01). Real-time X-ray stereoscopic vision can be easily achieved via the straightforward method described herein and has the potential to benefit patients during interventional procedures.
Using patient-derived iPSCs to develop humanized mouse models for chronic myelomonocytic leukemia and therapeutic drug identification, including liposomal clodronate
Authors: Taoka Kazuki , Arai Shunya , Kataoka Keisuke , Hosoi Masataka , Miyauchi Masashi , Yamazaki Sho , Honda Akira , Aixinjueluo Wei , Kobayashi Takashi , Kumano Keiki , Yoshimi Akihide , Otsu Makoto , Niwa Akira , Nakahata Tatsutoshi , Nakauchi Hiromitsu , Kurokawa Mineo 
Published: October 2018

AbstractChronic myelomonocytic leukemia (CMML) is an entity of myelodysplastic syndrome/myeloproliferative neoplasm. Although CMML can be cured with allogeneic stem cell transplantation, its prognosis is generally very poor due to the limited efficacy of chemotherapy and to the patient’s age, which is usually not eligible for transplantation. Comprehensive analysis of CMML pathophysiology and the development of therapeutic agents have been limited partly due to the lack of cell lines in CMML and the limited developments of mouse models. After successfully establishing patient’s derived disease-specific induced pluripotent stem cells (iPSCs) derived from a patient with CMML, we utilized these CMML-iPSCs to achieve hematopoietic re-differentiation in vitro, created a humanized CMML mouse model via teratomas, and developed a drug-testing system. The clinical characteristics of CMML were recapitulated following hematopoietic re-differentiation in vitro and a humanized CMML mouse model in vivo. The drug-testing system using CMML-iPSCs identified a MEK inhibitor, a Ras inhibitor, and liposomal clodronate as potential drugs for treating CMML. Clodronate is a drug commonly used as a bisphosphonate for osteoporosis. In this study, the liposomalization of clodronate enhanced its effectiveness in these assays, suggesting that this variation of clodronate may be adopted as a repositioned drug for CMML therapy.
Identification and Characterization of USP7 Targets in Cancer Cells
Authors: Georges Anna , Marcon Edyta , Greenblatt Jack , Frappier Lori 
Published: October 2018

AbstractThe ubiquitin specific protease, USP7, regulates multiple cellular pathways relevant for cancer through its ability to bind and sometimes stabilize specific target proteins through deubiquitylation. To gain a more complete profile of USP7 interactions in cancer cells, we performed affinity purification coupled to mass spectrometry to identify USP7 binding targets in gastric carcinoma cells. This confirmed reported associations of USP7 with USP11, PPM1G phosphatase and TRIP12 E3 ubiquitin ligase as well as identifying novel interactions with two DEAD/DEAH-box RNA helicases, DDX24 and DHX40. Using USP7 binding pocket mutants, we show that USP11, PPM1G, TRIP12 and DDX24 bind USP7 through its TRAF domain binding pocket, while DHX40 interacts with USP7 through a distinct binding pocket in the Ubl2 domain. P/A/ExxS motifs in USP11 and DDX24 that are critical for USP7 binding were also identified. Modulation of USP7 expression levels and inhibition of USP7 catalytic activity in multiple cells lines showed that USP7 consistently stabilizes DDX24, DHX40 and TRIP12 dependent on its catalytic activity, while USP11 and PPM1G levels were not consistently affected. Our study better defines the mechanisms of USP7 interaction with known targets and identifies DDX24 and DHX40 as new targets that are specifically bound and regulated by USP7.
Diagnosis of Idiopathic Pulmonary Fibrosis in a Possible Usual Interstitial Pneumonia Pattern: a meta-analysis
Authors: Kim Heekyung , Yoon Soon Ho , Hong Hyunsook , Hahn Seokyung , Goo Jin Mo 
Published: October 2018

AbstractThis study aimed to determine whether a surgical lung biopsy is essential for IPF diagnosis with the possible UIP CT pattern. We performed literature searches of the MEDLINE and EMBASE databases and included studies that conducted a radiologic-pathologic evaluation of IPF according to the 2011 guideline. Outcomes were pooled using a random-effects model. Twelve studies were included. Pooled proportions of IPF for a UIP pattern were 99% (95%CI, 93% to 100%; I2 = 51.7%) and for a possible UIP pattern were 94% (scenario inclusive of probable IPF; 95%CI, 87% to 99%; I2 = 82.9%) and 88% (scenario exclusive of probable IPF; 95%CI, 79% to 95%; I2 = 82.7%). The pooled percentage difference in the proportion of IPF between the UIP and possible UIP patterns was −2% (95%CI, −4% to 1%; I2 = 0.0%) in the former scenario and 4% (95%CI, 0% to 8%; I2 = 0.1%) in the latter scenario. The proportion of IPF with the possible UIP pattern was moderately correlated with the prevalence of IPF (correlation coefficient, 0.605; 95%CI, 0.550–0.860). There was a negligible pooled percentage difference in the proportion of IPF between the UIP and possible UIP patterns, indicating that IPF diagnosis can be confirmed without biopsy in suspected IPF cases with the possible UIP pattern.
Antiviral activity of the mineralocorticoid receptor NR3C2 against Herpes simplex virus Type 1 (HSV-1) infection
Authors: Haas Jürgen G. , Weber Julia , Gonzalez Orland , Zimmer Ralf , Griffiths Samantha J. 
Published: October 2018

AbstractAnalysis of a genome-scale RNA interference screen of host factors affecting herpes simplex virus type 1 (HSV-1) revealed that the mineralocorticoid receptor (MR) inhibits HSV-1 replication. As a ligand-activated transcription factor the MR regulates sodium transport and blood pressure in the kidney in response to aldosterone, but roles have recently been elucidated for the MR in other cellular processes. Here, we show that the MR and other members of the mineralocorticoid signalling pathway including HSP90 and FKBP4, possess anti-viral activity against HSV-1 independent of their effect on sodium transport, as shown by sodium channel inhibitors. Expression of the MR is upregulated upon infection in an interferon (IFN) and viral transcriptional activator VP16-dependent fashion. Furthermore, the MR and VP16, together with the cellular co-activator Oct-1, transactivate the hormone response element (HRE) present in the MR promoter and those of its transcriptional targets. As the MR induces IFN expression, our data suggests the MR is involved in a positive feedback loop that controls HSV-1 infection.
Thermal pace-of-life strategies improve phenological predictions in ectotherms
Authors: Struelens Quentin , Rebaudo François , Quispe Reinaldo , Dangles Olivier 
Published: October 2018

AbstractPhenological variability among populations is widespread in nature. A few predictive phenological models integrate intrapopulational variability, but none has ever explored the individual strategies potentially occurring within a population. The “pace-of-life” syndrome accounts for such individual strategies, but has yet to be explored under a phenological context. Here we integrated, for the first time, the slow-fast thermal strategies stemming from the “pace-of-life” into a mechanistic predictive framework. We obtained 4619 phenological observations of an important crop pest in the Bolivian Andes by individually following 840 individuals under five rearing temperatures and across nine life stages. The model calibrated with the observed individual “pace-of-life” strategies showed a higher accuracy in phenological predictions than when accounting for intrapopulational variability alone. We further explored our framework with generated data and suggest that ectotherm species with a high number of life stages and with slow and/or fast individuals should exhibit a greater variance of populational phenology, resulting in a potentially longer time window of interaction with other species. We believe that the “pace-of-life” framework is a promising approach to improve phenological prediction across a wide array of species.
Plasma lipidomic analysis reveals strong similarities between lipid fingerprints in human, hamster and mouse compared to other animal species
Authors: Kaabia Zied , Poirier Julie , Moughaizel Michelle , Aguesse Audrey , Billon-Crossouard Stéphanie , Fall Fanta , Durand Manon , Dagher Elie , Krempf Michel , Croyal Mikaël 
Published: October 2018

AbstractCardiovascular diseases are often associated with impaired lipid metabolism. Animal models are useful for deciphering the physiological mechanisms underlying these pathologies. However, lipid metabolism is contrasted between species limiting the transposition of findings from animals to human. Hence, we aimed to compare extended lipid profiles of several animal species to bring new insights in animal model selections. Human lipid phenotype was compared with those of 10 animal species. Standard plasma lipids and lipoprotein profiles were obtained by usual methods and lipidomic analysis was conducted by liquid chromatography-high-resolution mass spectrometry (LC-HRMS). As anticipated, we found contrasted lipid profiles between species. Some of them exhibited similar plasma lipids to human (non-human primate, rat, hamster, pig), but only usual lipid profiles of pigs were superimposable with human. LC-HRMS analyses allowed the identification of 106 other molecular species of lipids, common to all samples and belonging to major lipid families. Multivariate analyses clearly showed that hamster and, in a lower extent mouse, exhibited close lipid fingerprints to that of human. Besides, several lipid candidates that were previously reported to study cardiovascular diseases ranged similarly in human and hamster. Hence, hamster appeared to be the best option to study physiological disturbances related to cardiovascular diseases.
Behavior Assessment Battery: Normative and Psychometric Investigation Among Polish Adults Who Do and Do Not Stutter
Authors: Węsierska Katarzyna , Vanryckeghem Martine , Krawczyk Aleksandra , Danielowska Marta , Faściszewska Maria , Tuchowska Jadwiga 
Published: October 2018

PurposeThe aim of this study was to establish normative data for Polish persons who stutter (PWS) and persons who do not stutter (PWNS) with the adapted version of the Behavior Assessment Battery (BAB; Vanryckeghem & Brutten, 2018) for adults and to investigate whether or not gender and age influenced the results. In addition, the BAB subtests' reliability and validity were examined.MethodA Polish version of the BAB test procedures, including the Speech Situation Checklist–Emotional Reaction, Speech Situation Checklist–Speech Disruption, Behavior Checklist, and Communication Attitude Test for Adults Who Stutter (Vanryckeghem & Brutten, 2018), were individually administered to 123 adults who stutter and 151 adults who do not stutter between the ages of 17 and 70.ResultsPWS scored statistically significantly higher on all BAB subtests compared to PWNS. No significant between-groups gender or age difference was detected. The test data indicate high internal validity. The test items correlate well with the total score and significantly differentiate the two groups. The test battery validly differentiates PWS from PWNS with high accuracy.ConclusionsThe Polish version of the BAB for adults confirms data collected in previous cross-cultural investigations. The results once again differentiate PWS from PWNS in a powerful way. Compared to PWNS, PWS report significantly more anxiety and speech breakdown when speaking in particular speech situations. They also report their attitude toward speech to be significantly more negative compared to the typical speaker and indicate using a significant number of coping behaviors to avoid or escape difficult speaking conditions. The BAB test battery, in its totality, assists in multidimensional assessment, pre- and posttreatment comparison, and identification of treatment targets based on the answers of PWS to each test item.
Psychokardiologische Grundlagen: Wie schädigt Stress das Herz-Kreislauf-System?
Authors: Kuntz-Hehner Stefanie , Angerer Peter 
Published: October 2018

ZusammenfassungAkuter, wiederholter und chronischer Stress in verschiedenen Lebensbereichen erhöht das Risiko für die Entstehung, die klinische Erstmanifestation und eine ungünstige Prognose einer kardiovaskulären Erkrankung. Diese epidemiologisch auf breiter Basis nachgewiesenen Zusammenhänge lassen sich durch biologische Stressreaktionen, emotionale Reaktionen und Verhalten plausibel erklären. Aus physiologischen Stressreaktionen entstehen pathophysiologische Abläufe, die schließlich die Atherogenese fördern und klinische Ereignisse wie akute Koronarsyndrome, Herzinsuffizienz, Herzrhythmusstörungen oder Schlaganfälle auslösen können. Dabei können sich stressinduzierte intermediäre Erkrankungen wie Depressionen, arterielle Hypertonie oder Diabetes mellitus ihrerseits langfristig auf weitere kardiovaskuläre Krankheitsmanifestationen auswirken. Das Verständnis dieser pathophysiologischen Wege bietet somit Ansatzpunkte für gezielte Interventionen.
Außerberuflicher Stress und kardiovaskuläre Erkrankungen
Authors: Sperlich Stefanie 
Published: October 2018

ZusammenfassungDieser Beitrag resümiert die Bedeutung außerberuflicher Stressoren für kardiovaskuläre Erkrankungen im Hinblick auf die psychosozialen Faktoren „soziale Beziehungen“, „kritische Lebensereignisse“, „Arbeitslosigkeit und finanzielle Belastungen“, „soziale Diskriminierung“, „unbezahlte Pflegetätigkeit“, „Haus- und Familienarbeit“ sowie „Vereinbarkeit von Beruf und Familie“, die auf der Grundlage einer systematischen Literaturrecherche ermittelt wurden. Die Sichtung der Studien ergab eine hohe empirische Evidenz für einen Zusammenhang zwischen unzureichender sozialer Unterstützung und kardiovaskulären Erkrankungen, während die empirische Beweislage insbesondere für soziale Diskriminierungserfahrungen, unbezahlte Pflege- bzw. Haus- und Familienarbeit sowie die Vereinbarkeit von Beruf und Familie geringer ausfällt. Die vorliegenden Ergebnisse verweisen jedoch grundsätzlich auf die Bedeutung dieser Stressoren für die Entstehung von kardiovaskulären Risikofaktoren sowie die Inzidenz und Prognose von kardiovaskulären Erkrankungen. Weiterführende Studien sind angezeigt, um die Evidenzlage zu fundieren und weiterführende Aussagen über genderspezifische Einflüsse und besonders belastete Personengruppen treffen zu können.
Stress in der Prävention und Rehabilitation der koronaren Herzkrankheit
Authors: Marx Roger , Bassenge Dirk 
Published: October 2018

ZusammenfassungIn den Forschungen der letzten Jahre hat sich Stress als ein eigenständiger Risikofaktor für die Entwicklung und das Fortschreiten der koronaren Herzkrankheit herausgestellt. Die transaktionale Stresstheorie beleuchtet die stressbezogenen Transaktionen zwischen Person und Umwelt und legt den Schwerpunkt auf die individuellen Bewertungs- und Bewältigungsprozesse. In der Prävention stellt Stress einen Risikofaktor dar, der sowohl im Rahmen der Verhaltensprävention (Verbesserung der eigenen Gesundheitskompetenz) als auch der Verhältnisprävention (Berücksichtigung der sozialen Situation) modifiziert werden kann. In der kardiologischen Rehabilitation stehen weniger die strukturellen als die interpersonalen bzw. die personalen Interventionsmaßnahmen im Vordergrund. Es wird für alle Patienten ein niederschwelliges Angebot vorgehalten, in dem Stressbewältigungskompetenzen gefördert werden. Darüber hinaus haben sich vertiefende Angebote mit verschiedenen empirisch evaluierten Programmen etabliert. Hier wird ein multimodales Stressbewältigungstraining absolviert, das sich an moderne Stresstheorien anlehnt. Es sollen Verhaltensmuster erlernt werden, die in den Alltag transferiert werden.
Asthma neuraltherapeutisch behandeln
Authors: Wander Rainer 
Published: October 2018

ZusammenfassungJeder segmentale Reiz bedeutet auch segmentaler Stress und induziert eine neurogene Entzündung. Nach den Mechanismen der Stressverarbeitung kann dies
zeitabhängig krankmachen. Das in der Neuraltherapie favorisierte Lokalanästhetikum Procain kann mit seinen Spaltprodukten im Gewebe eine echte antientzündliche
Wirkung und damit eine Antistresswirkung entfalten und ist damit allen anderen Lokalanästhetika überlegen.Am Beispiel von Asthma werden neuraltherapeutische Strategien zur Behandlung von Atemwegserkrankungen vorgestellt: In der Praxis hat sich ein 3-stufiges Vorgehen
bewährt: die Behandlung im Segment, im erweiterten Segment sowie die Behandlung über das Störfeld. Zwei Fälle aus der Praxis zeigen exemplarisch die möglichen
Behandlungserfolge.
Asthma und Vitamin D
Authors: Spitz Jörg 
Published: October 2018

ZusammenfassungDeutschlandweit sind über 3 Millionen Erwachsene von Asthma bronchiale und über 12 Millionen von allergischer Rhinitis betroffen. Studien belegen, dass Vitamin D
eng mit der Regulation des Immunsystems verbunden ist. Die gezielte Beseitigung eines Vitamin-D-Mangels führt daher zu deutlich positiven Auswirkungen bei
diesen Erkrankungen sowie bei Infektionskrankheiten und Autoimmunerkrankungen. Die Voraussetzung für eine erfolgreiche Vorbeugung und Behandlung ist eine
tägliche Dosis des Sonnenhormons, die den Vitamin-D-Spiegel ganzjährig in einem Bereich von 40–60 ng/ml hält.