bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrative analysis of differentially expressed genes and miRNAs predicts complex T3-mediated protective circuits in a rat model of cardiac ischemia reperfusion
Authors: Forini Francesca , Nicolini Giuseppina , Kusmic Claudia , D’Aurizio Romina , Rizzo Milena , Baumgart Mario , Groth Marco , Doccini Stefano , Iervasi Giorgio , Pitto Letizia 
Published: September 2018

AbstractThyroid hormone (T3) dyshomeostasis in the cardiac ischemia-reperfusion (IR) setting negatively impacts on mitochondria function and extracellular matrix remodeling. The modulation of cardiac miRNAs may represent the underlying molecular mechanisms, but a systems biology perspective investigating this critical issue in depth is still lacking. A rat model of myocardial IR, with or without an early short-term T3-replacement, was used to predict putative T3-dependent miRNA-gene interactions targeted to mitochondria quality control and wound healing repair. As evidenced by mRNA and miRNA expression profiling, the T3 supplementation reverted the expression of 87 genes and 11 miRNAs that were dysregulated in the untreated group. In silico crossing and functional analysis of the T3-associated differentially expressed transcripts, identified a signature of interconnected miRNA-gene regulatory circuits that confer resistance to noxious cascades of acute stress. In this network the T3-down-regulated Tp53, Jun and Sp1 transcription factors emerge as critical nodes linking intrinsic cell death and oxidative stress pathways to adverse remodeling cascades. The data presented here provide a novel insight into the molecular basis of T3 cardioprotection in the early post-IR phase and highlight the contribution of a previously unappreciated complex T3-regulatory network that may be helpful in translating T3 replacement into clinical practice.
The sense of agency shapes body schema and peripersonal space
Authors: D’Angelo Mariano , di Pellegrino Giuseppe , Seriani Stefano , Gallina Paolo , Frassinetti Francesca 
Published: September 2018

AbstractBody schema, a sensorimotor representation of the body used for planning and executing movements, is plastic because it extends by using a tool to reach far objects. Modifications of peripersonal space, i.e., a functional representation of reach space, usually co-occur with body schema changes. Here, we hypothesized that such plastic changes depend on the experience of controlling the course of events in space trough one’s own actions, i.e., the sense of agency. In two experiments, body schema and peripersonal space were assessed before and after the participants’ sense of agency over a virtual hand was manipulated. Body schema and peripersonal space enlarged or contracted depending on whether the virtual hand was presented in far space, or closer to the participants’ body than the real hand. These findings suggest that body schema and peripersonal space are affected by the dynamic mapping between intentional body movements and expected consequences in space.
Nanoparticles reveal Extreme Size-Sorting and Morphologies in Complex Coacervate Superstructures
Authors: ten Hove Jan Bart , van Oosterom Matthias N. , van Leeuwen Fijs W. B. , Velders Aldrik H. 
Published: September 2018

AbstractWe here provide detailed insight in self-assembled complex coacervate systems exploiting gold nanoparticles for cryoTEM contrast. Nanoparticle-containing dendrimicelles are formed from fifth-generation dendrimer-encapsulated nanoparticles (DENs) and dendrimer-stabilized nanoparticles (DSNs). The complex coacervate structures self-organize in biconcave thin water layers into size-sorted monolayer superstructures. The embedded nanoparticles are a straightforward tool to visualize dendrimicelles and determine the aggregation number and polydispersity. The superstructure shows extreme size-sorting patterns which, contrary to related systems with higher generation dendrimers, consists not only of dendrimicelles but also much bigger complex coacervate nanoassemblies, such as vesicles.
A combination of curcumin and oligomeric proanthocyanidins offer superior anti-tumorigenic properties in colorectal cancer
Authors: Ravindranathan Preethi , Pasham Divya , Balaji Uthra , Cardenas Jacob , Gu Jinghua , Toden Shusuke , Goel Ajay 
Published: September 2018

AbstractCombining anti-cancer agents in cancer therapies is becoming increasingly popular due to improved efficacy, reduced toxicity and decreased emergence of resistance. Here, we test the hypothesis that dietary agents such as oligomeric proanthocyanidins (OPCs) and curcumin cooperatively modulate cancer-associated cellular mechanisms to inhibit carcinogenesis. By a series of in vitro assays in colorectal cancer cell lines, we showed that the anti-tumorigenic properties of the OPCs-curcumin combination were superior to the effects of individual compounds. By RNA-sequencing based gene-expression profiling in six colorectal cancer cell lines, we identified the cooperative modulation of key cancer-associated pathways such as DNA replication and cell cycle pathways. Moreover, several pathways, including protein export, glutathione metabolism and porphyrin metabolism were more effectively modulated by the combination of OPCs and curcumin. We validated genes belonging to these pathways, such as HSPA5, SEC61B, G6PD, HMOX1 and PDE3B to be cooperatively modulated by the OPCs-curcumin combination. We further confirmed that the OPCs-curcumin combination more potently suppresses colorectal carcinogenesis and modulated expression of genes identified by RNA-sequencing in mice xenografts and in colorectal cancer patient-derived organoids. Overall, by delineating the cooperative mechanisms of action of OPCs and curcumin, we make a case for the clinical co-administration of curcumin and OPCs as a treatment therapy for patients with colorectal cancer.
Late Pleistocene glacial transitions in North America altered major river drainages, as revealed by deep-sea sediment
Authors: Fildani Andrea , Hessler Angela M. , Mason Cody C. , McKay Matthew P. , Stockli Daniel F. 
Published: September 2018

AbstractSediment eroded from continents during ice ages can be rapidly (<104 years) transferred via rivers to the deep-sea and preserved in submarine fans, becoming a viable record of landscape evolution. We applied chemical weathering proxies and zircon geo-thermo-chronometry to late Pleistocene sediment recovered from the deep-sea Mississippi fan, revealing interactions between the Laurentide ice sheet (LIS) and broader Mississippi–Missouri catchment between ca. 70,000 and 10,000 years ago (70 to 10 ka). Sediment contribution from the Missouri catchment to the Mississippi fan was low between 70 and 30 ka but roughly doubled after the Last Glacial Maximum (LGM). Therefore, pre-LGM glacial advance profoundly altered the vast Missouri drainage through ice dams and/or re-routing of the river, thereby controlling the transfer of continental debris and freshwater toward southern outlets.
Different multi-year mean temperature in mid-summer of South China under different 1.5 °C warming scenarios
Authors: Qu Xia , Huang Gang 
Published: September 2018

AbstractThe Paris Agreement proposed a goal of “pursuing efforts to limit the temperature increase to 1.5 °C above pre-industrial levels”. The Community Earth System Model, version 1, with the Community Atmosphere Model, version 5 (CESM1-CAM5), designed a set of experiments that fulfilled the 1.5 °C warming goal. By analyzing the outputs, this study aims to present projections associated with warming in South China (SC). Interestingly, if the global mean temperature (GMT) overshoots to 1.7 °C above the pre-industrial levels in 2050 and back to 1.5 °C by 2100, additional warming in the SC mid-summer will occur when approaching 2100 compared to that in the scenario under which the GMT stabilizes at an increase of 1.5 °C after the mid-2040 s. In the final 1 to 3 decades of 21st century in most parts of SC, the multi-year mean warming differences, as well as the difference of extreme hot days, between the two scenarios are significant among the ensembles in mid-summer. Under the scenario in which the GMT overshoots an increase of 1.5 °C, the decrease of mid-level clouds leads to increased downwards solar radiation in the SC and warms the surface, resulting in increases in both outgoing longwave radiation and latent heat flux into the atmosphere and maintenance of the surface balance of the heat budget.
Randomized trial examining effectiveness of lifestyle intervention in reducing gestational diabetes in high risk Chinese pregnant women in Hong Kong
Authors: Chan Ruth Suk-Mei , Tam Wing-Hung , Ho Ivan Chak-Hang , Kwan Macy Wai-Chi , Li Liz Sin , Sea Mandy Man-Mei , Woo Jean 
Published: September 2018

AbstractGestational diabetes mellitus (GDM) is of public health concern. This trial examined whether a clinically proven lifestyle modification program (LMP) in early pregnancy was superior to routine antenatal care in improving GDM, maternal and infant outcomes. Chinese pregnant women at risk of GDM (n = 220) were recruited at or before 12-week gestation and randomized to either a LMP group or a routine care control group. Eighty subjects completed a dietitian-led LMP including dietary and exercise components from early pregnancy till 24-week gestation. Data were compared with those of 86 control subjects. Twenty three (26.7%) control subjects and 20 (25.0%) LMP subjects developed GDM (p = 0.798). The proportion of infants born large for gestational age and macrosomia was similar between groups. The LMP group showed a lower proportion of excessive gestational weight gain (GWG). Subgroup analysis suggested that those with higher LMP adherence showed more desirable dietary composition and energy intake, and lower proportion of excessive GWG compared with the low LMP adherence group and the control group. The potential effect of LMP on GDM and other maternal and infant outcomes, in particular GWG, as well as barriers for making lifestyle changes warrant further investigations (ClinicalTrials.gov NCT02368600).
Recognition of Schizophrenia with Regularized Support Vector Machine and Sequential Region of Interest Selection using Structural Magnetic Resonance Imaging
Authors: Chin Rowena , You Alex Xiaobin , Meng Fanwen , Zhou Juan , Sim Kang 
Published: September 2018

AbstractStructural brain abnormalities in schizophrenia have been well characterized with the application of univariate methods to magnetic resonance imaging (MRI) data. However, these traditional techniques lack sensitivity and predictive value at the individual level. Machine-learning approaches have emerged as potential diagnostic and prognostic tools. We used an anatomically and spatially regularized support vector machine (SVM) framework to categorize schizophrenia and healthy individuals based on whole-brain gray matter densities estimated using voxel-based morphometry from structural MRI scans. The regularized SVM model yielded recognition accuracy of 86.6% in the training set of 127 individuals and validation accuracy of 83.5% in an independent set of 85 individuals. A sequential region-of-interest (ROI) selection step was adopted for feature selection, improving recognition accuracy to 92.0% in the training set and 89.4% in the validation set. The combined model achieved 96.6% sensitivity and 74.1% specificity. Seven ROIs were identified as the optimal discriminatory subset: the occipital fusiform gyrus, middle frontal gyrus, pars opercularis of the inferior frontal gyrus, anterior superior temporal gyrus, superior frontal gyrus, left thalamus and left lateral ventricle. These findings demonstrate the utility of spatial and anatomical priors in SVM for neuroimaging analyses in conjunction with sequential ROI selection in the recognition of schizophrenia.
The m6A-methylase complex recruits TREX and regulates mRNA export
Authors: Lesbirel Simon , Viphakone Nicolas , Parker Matthew , Parker Jacob , Heath Catherine , Sudbery Ian , Wilson Stuart A. 
Published: September 2018

AbstractN6-methyladenosine (m6A) is the most abundant internal modification of eukaryotic mRNA. This modification has previously been shown to alter the export kinetics for mRNAs though the molecular details surrounding this phenomenon remain poorly understood. Recruitment of the TREX mRNA export complex to mRNA is driven by transcription, 5′ capping and pre-mRNA splicing. Here we identify a fourth mechanism in human cells driving the association of TREX with mRNA involving the m6A methylase complex. We show that the m6A complex recruits TREX to m6A modified mRNAs and this process is essential for their efficient export. TREX also stimulates recruitment of the m6A reader protein YTHDC1 to the mRNA and the m6A complex influences the interaction of TREX with YTHDC1. Together our studies reveal a key role for TREX in the export of m6A modified mRNAs.
Huayu Tongmai Granules protects against vascular endothelial dysfunction via up-regulating miR-185 and down-regulating RAGE
Authors: Liu Xiaoming , Wang Dongli , Yang Xiaoni , Lei Lei 
Published: September 2018

Objective: Receptor of advanced glycation end products (RAGE) is a membrane protein that contributes to the initiation and progression of diabetic vascular complications, which is reported as a target of miR-185. Huayu Tongmai Granules is a Chinese herbal compound that is capable of treating diabetic angiopathy. The present study was designed to explore the molecular biological mechanism by which Huayu Tongmai Granules protects against diabetic angiopathy.
Methods: The rat model of diabetes and hyperglucose cell model were established. The blood glucose was detected to verify whether the model was successfully established. Besides, serum nitric oxide (NO) and reactive oxygen species (ROS) concentrations of the rats in each group were determined. The quantitative real-time PCR analysis was performed to examine the mRNA expression levels of miR-185 and other miRNAs in femoral artery of rats or human umbilical vein endothelial cell line. Additionally, the protein levels of RAGE or Bax were determined using Western blotting. Cell apoptosis was determined by terminal dUTP nick-end labeling assay or flow cytometry.
Results: In the present study, we found that Huayu Tongmai Granules significantly decreased blood glucose and serum ROS and up-regulated serum NO concentration. MiR-185 was low-expressed in diabetic rats; however, Huayu Tongmai Granules intervention up-regulated miR-185. Stable overexpression of miR-185 directly suppressed the expression of RAGE and further suppressed endothelial cell apoptosis.:
ConclusionHuayu Tongmai Granules appears to have a therapeutic effect on diabetic angiopathy that is most probably mediated by miR-185/RAGE axis.
Ernährungstherapie der nichtalkoholischen
Fettleber
Authors: Worm Nicolai 
Published: September 2018

ZusammenfassungLange Zeit unterschätzt, hat sich die nichtalkoholische Fettlebererkrankung
innerhalb von 3 Jahrzehnten zur am weitesten verbreiteten Lebererkrankung in den
Industrienationen entwickelt 1. Mittlerweile
erlangt sie epidemische Ausmaße und ist damit zu einer bedrohlichen
Volkskrankheit geworden.
Inherited Forms of Primary Hyperaldosteronism: New Genes, New Phenotypes and Proposition of A New Classification
Authors: Perez-Rivas Luis , Williams Tracy , Reincke Martin 
Published: September 2018

AbstractPrimary aldosteronism is a common cause of endocrine hypertension. It results from the excess production of aldosterone by the adrenal cortex and is related to increased morbidity and mortality. Most cases of PA are sporadic but inherited patterns of the disease have been reported in the literature. Four forms of familial hyperaldosteronism (FH-I- FH-IV) are currently recognized, and the genetic basis has been clarified in recent years. In FH-I patients, aldosterone excess is produced by a CYP11B1/CYP11B2 fusion gene and it is suppressed by glucocorticoid treatment. FH-II is caused by mutations in the inwardly rectifying chloride channel CLCN2. FH-III is caused by mutations in KCNJ5, a gene coding for an inward rectifier K+ channel and mutations in the T-type calcium channel subunit CACNA1H cause FH-IV. In this review we summarize the knowledge on inherited forms of primary aldosteronism, the genetic alterations that cause them and the implications it may have for the classification. Based on current evidence, we propose the term “familial hyperaldosteronism” to refer only to inherited forms of primary aldosteronism with a known genetic basis.
Rosehip Oil Promotes Excisional Wound Healing by Accelerating the Phenotypic Transition of Macrophages
Authors: Lei Zhiyong , Cao Zhijian , Yang Zaiwang , Ao Mingzhang , Jin Wenwen , Yu Longjiang 
Published: September 2018

AbstractPoor wound healing is a major and global threat to public health. Efforts have been made to better understand the underlying mechanisms and develop effective remedies, though the advancements that have been made are still limited. As there are no effective and generally applicable therapies available for skin injuries and fibrosis, it is urgent to develop new drugs and therapies that facilitate wound healing and effectively improve scars. In this study, GC-MS analysis was performed to identify the chemical composition of rosehip oil. The excisional wound healing model and the carrageenan-induced paw edema method were respectively applied to evaluate the wound healing activity and anti-inflammatory activity of rosehip oil. Hematoxylin and eosin staining was used to assess the pathological changes of sections, and Sirius-red staining was performed to analyze the ratio of collagen I/III in wound tissues. Immunohistological staining for CD68, CCR7 (CD197), CD163, TGF-β1, and α-SMA was applied to determine the macrophage phenotypes transition (M1-to-M2) and demonstrate the scar-improving efficacy of rosehip oil on wound healing. Results showed that rosehip oil significantly promoted wound healing and effectively improved scars. This efficacy might be exerted by accelerating the macrophage phenotypes transition and inhibiting the process of epithelial-mesenchymal transition.
Fetal Congenital Pulmonary Airway Malformation: The Role of an Objective Measurement of Cardiomediastinal Shift
Authors: Sparks Teresa , Gosnell Kristen , Blat Cinthia , Norton Mary , Lee Hanmin , Gonzalez-Velez Juan , Goldstein Ruth , Shulman Rachel 
Published: September 2018

Objective To examine the relationship between cardiomediastinal shift angle (CMSA) and adverse perinatal outcomes and hydrops in cases of congenital pulmonary airway malformation (CPAM).
Study Design This retrospective study evaluated CPAM cases referred to our institution from 2008 to 2015. The primary outcome was a composite score for adverse perinatal outcome. CMSA was measured for each case and evaluated for its association with the primary outcome. The prediction accuracy of CMSA for adverse perinatal outcome was assessed using receiver operator characteristic (ROC) curves.
Results Eighteen (21.2%) of the 85 cases experienced an adverse perinatal outcome. Increases in CMSA were associated with adverse perinatal outcomes and hydrops in bivariate analyses. Adjusted analyses found each 10-degree increase in CMSA to be associated with increased odds of an adverse perinatal outcome (adjusted odds ratio [aOR] 2.2, 95% confidence interval [CI]: 1.4–3.3) and hydrops (aOR 3.0, 95% CI: 1.5–6.1). CMSA performed well and was comparable to CPAM volume ratio in predicting adverse perinatal outcomes (area under the curve 0.81 and 0.84, respectively).
Conclusion We describe a novel measurement of mediastinal shift in cases of CPAM and its relationship with adverse perinatal outcomes and hydrops. These findings may shape the evaluation and management of CPAMs, improve our understanding of their prognosis, and influence patient counseling.
Olfactory Nerve Schwannoma: A Case Report and Review of the Literature
Authors: Taha Mahmoud , AlBakry Amr , ElSheikh Magdy , AbdelBary Tarek 
Published: September 2018

AbstractSchwannomas are benign tumors, which arise from the Schwann cells of the central or peripheral nerves. They form 8% of all intracranial tumors and most of the cases arise from vestibular division of the 8th cranial nerve. Rare cases are shown to arise from the olfactory or optic nerve, being devoid of myelin sheath. Up to date and according to our best of knowledge, 66 cases have been reported till now. Here we present a review of the literature and a case report of a 56-year-old male with an accidently discovered anterior cranial fossa schwannoma, following a road traffic accident. Tumor was completely excised, using a right frontal approach. Histopathology revealed Antoni-A cellular pattern. Although rare, but olfactory nerve schwannomas should be included in the differential diagnosis in anterior cranial fossa space occupying lesions, and the approach should be designed taking into consideration, this rare entity.
Papaverine and its derivatives radiosensitize solid tumors by inhibiting mitochondrial metabolism
Authors: Benej Martin , Hong Xiangqian , Vibhute Sandip , Scott Sabina , Wu Jinghai , Graves Edward , Le Quynh-Thu , Koong Albert C. , Giaccia Amato J. , Yu Bing , Chen Ching-Shih , Papandreou Ioanna , Denko Nicholas C. 
Published: September 2018

Significance
Oxygen tension plays a critical role in the response to radiation therapy (RT). Here we show that hypoxic tumors can be sensitized to RT by targeting mitochondrial respiration. We identified the 150-year-old FDA-approved drug papaverine as a mitochondrial complex I inhibitor. A single dose of the drug prior to RT alleviates hypoxia in model tumors and strikingly enhances the response to RT. Well-oxygenated normal tissues are not radiosensitized. Removal of papaverine’s phosphodiesterase 10A inhibitory activity by structural modification has identified potentially safer generation of complex I-inhibiting radiosensitizers.
mRNA vaccination with charge-altering releasable transporters elicits human T cell responses and cures established tumors in mice
Authors: Haabeth Ole A. W. , Blake Timothy R. , McKinlay Colin J. , Waymouth Robert M. , Wender Paul A. , Levy Ronald 
Published: September 2018

Significance
The RNA delivery field is mostly focused on lipid nanoparticles (LNPs). Although promising, LNPs have several limitations with respect to pharmacokinetics, biodistribution, and toxicity. The mechanism of RNA charge-altering releasable transporters (CART) delivery and release is unique. It proceeds dynamically with a controllable change in physical properties. Differing from all mRNA delivery systems, a key attribute of CARTs is a charge-altering degradation mechanism, which transforms the initial polycationic CART into neutral byproducts, thereby enabling endosomal escape, release, and subsequent translation of the polyanionic mRNA cargo. With this study, we introduce a potentially general approach to therapeutic vaccination enabled by a dynamic drug-delivery system (mRNA-CART) and demonstrate its utility in suppressing tumor formation and in eliminating established tumors.