bluuebunny/crossref_metadata_2025_split huggingface dataset:
Outcomes Using High KDPI Kidneys in Recipients Over 65 y of Age
Authors: Goussous Naeem , De Leon Fransia , Alghannam Karima , Howard Brian C. , Than Peter A. , Wang Aileen X , Sageshima Junichiro , Perez Richard V. 
Published: November 2024

Background.
Kidney transplantation has been shown to improve life expectancy when compared with remaining on dialysis. However, there is an ever-expanding shortage of available organs for transplantation. The use of high kidney donor profile index (KDPI; >85) kidneys is 1 strategy to address this shortage. The current study aims to evaluate the advantage of accepting a high KDPI versus a low KDPI kidney (KDPI ≤85) in patients 65 y or older.

Methods.
A single-center retrospective review of all patients, ages 65 y or older, who underwent deceased donor kidney transplantation between 2010 and 2020 was performed. Outcomes and wait times of recipients undergoing low KDPI (KDPI ≤85) versus high KDPI (KDPI >85) kidney transplantation were compared. Significance was defined as P < 0.05.

Results.
Four hundred ninety-two patients were identified; 317 (64.4%) were men with a median age at transplantation of 69 y. Four hundred five patients received low KDPI kidneys, whereas the remaining received high KDPI grafts. High KDPI kidneys were procured from older donors (60 versus 47, P < 0.001) and had shorter cold ischemic time (25.0 versus 28.3 h, P = 0.01) compared with low KDPI kidneys. There was no significant difference in graft and patient survival between low and high KDPI recipients, with 85.1% and 86.2% grafts functioning at a follow-up of 4.85 (2.9–7.0) y, respectively. Pretransplant wait time was significantly shorter in the high KDPI group (2.7 [1.8–4.1] versus 3.5 [2.3–4.8] y; P = 0.004).

Conclusions.
In patients 65 y or older undergoing deceased donor kidney transplantation, high KDPI kidneys may offer shorter pretransplant waiting times without compromising graft or patient survival.
HIF2α negatively regulates MYCN protein levels and promotes a low-risk noradrenergic phenotype in neuroblastoma
Authors: Yuan Juan , Maitra Subhamita , Antoniou Eirini , Zhu Jiacheng , Li Wenyu , Demirel Ilknur Safak , Toskas Kostantinos , Martinez Iria Laura , Ozcimen Lacin , Lindehell Henrik , Kogner Per , Bedoya-Reina Oscar C. , Schlisio Susanne , Holmberg Johan 
Published: November 2024

AbstractThe role of HIF2α, encoded byEPAS1, in neuroblastoma remains controversial. Here we demonstrate that induction of high levels of HIF2α in MYCN-amplified neuroblastoma cells results in a rapid and profound reduction of the oncoprotein MYCN. This is followed by an upregulation of genes characteristic of noradrenergic cells in the adrenal medulla. Additionally, upon induction of HIF2α, the proliferation rate drops substantially, and cells develop elongated neurite-like protrusions, indicative of differentiation.In vivoHIF2α induction in established xenografts significantly attenuates tumour growth. Notably, analysis of sequenced neuroblastoma patient samples, revealed a negative correlation betweenEPAS1andMYCNexpression and a strong positive correlation betweenEPAS1expression, high expression levels of noradrenergic markers, and improved patient outcome. This was paralleled by analysis of human adrenal medulla datasets whereinEPAS1expression was prominent in populations with high expression levels of genes characteristic of noradrenergic chromaffin cells. Our findings show that high levels of HIF2α in neuroblastoma, leads to drastically reduced MYCN protein levels, cell cycle exit, and noradrenergic cell differentiation. Taken together, our results challenge the dogma that HIF2α acts as an oncogene in neuroblastoma and rather suggest that HIF2α has potential tumour suppressor capacity in this particular disease.Significance statementHIF2α has been proposed as a neuroblastoma oncogene and a tractable target for clinical intervention, this has been questioned by several studies. Thus, it is necessary to move beyond correlative studies and better determine the function of HIF2α in neuroblastoma. Our study shows that induced expression of HIF2α inMYCN-amplified neuroblastoma substantially reduces MYCN protein levels and attenuates proliferation while it induces several features of noradrenergic differentiation and impedes xenograft tumour growth. Together with bioinformatic analysis of sequenced neuroblastoma patient samples and the developing human adrenal medulla, this couples HIF2α to low-risk neuroblastoma with a substantially better patient outcome. Thus, in neuroblastoma HIF2α exhibit tumour suppressor capacity rather than oncogenic capacity.
Three STEPs forward: A trio of unexpected structures of PTPN5
Authors: Guerrero Liliana , Ebrahim Ali , Riley Blake T. , Kim Sean H. , Bishop Anthony C. , Wu Jiaqian , Han Ye Na , Tautz Lutz , Keedy Daniel A. 
Published: November 2024

AbstractProtein tyrosine phosphatases (PTPs) play pivotal roles in myriad cellular processes by counteracting protein tyrosine kinases. Striatal-enriched protein tyrosine phosphatase (STEP, PTPN5) regulates synaptic function and neuronal plasticity in the brain and is a therapeutic target for several neurological disorders. Here, we present three new crystal structures of STEP, each with unexpected features. These include high-resolution conformational heterogeneity at multiple sites, and a highly coordinated citrate molecule in the active site, a previously unseen conformational change at an allosteric site, an intramolecular disulfide bond that was characterized biochemically but had never been visualized structurally, and two serendipitous covalent ligand binding events at surface-exposed cysteines that are nearly or entirely unique to STEP among human PTPs. Together, our results offer new views of the conformational landscape of STEP that may inform structure-based design of allosteric small molecules to specifically inhibit this biomedically important enzyme.
Exploring the Hypothetical Role ofBacteroidesSpecies in Depression Progression: Insights from Metagenomic Analysis
Authors: Li Zihua , Sun Jingxi , Yang Jinlong , Han Panpan , Min Liping , Cheng Yongkang , Zou Yuanqiang , Liu Zhuhua 
Published: November 2024

AbstractDepression, a psychiatric disorder with significant morbidity and mortality, has a complex etiology. Recent advances in microbiome research have highlighted the potential role of gut microbiota in depression pathogenesis. This study utilized shotgun metagenomic sequencing to compare the fecal microbiota of 28 depression patients and 26 healthy individuals. Significant differences in gut microbiota composition were observed between the two groups. We generated 350 non-redundant high-quality metagenome-assembled Genomes (MAGs) by binning and conducted comparisons between the depression and control groups. Notably, we found that the MAGs enriched in people with depression mostly belonged toBactendicating a close link betweenBacteroidesabundance and the development of depression, suggesting thatBacteroidesmight be a potential culprit for deroides, ipression. In the depression group, we found that the module of nitric oxide synthesis was remarkably enriched, and allBacteroidesMAGs were annotated to nitric oxide synthase, suggesting that increased levels of Bacteroides may contribute to elevated nitric oxide synthesis. Specifically, the mean relative abundance about the genomes ofBacteroides xylanisolvens,Bacteroides caccae,Bacteroides fragilis,Bacteroides stercorisandBacteroides ovatusshowed strong discriminatory power in distinguishing depressed patients from healthy individuals (AUC=0.834). This research shed light on the potential role of gut microbiota in depression and highlights specific metabolic pathways and microbial markers for further investigation.ImprotanceThis research highlighted significant differences in the composition and function of gut microbiota between individuals with depression and healthy individuals, particularly the enrichment ofBacteroidesMAGs in depression patients. The upregulation of the nitric oxide synthesis pathway associated with these MAGs belong toBacteroidesin the gut of depression patients had also been observed. The mean relative abundance of a specific set ofBacteroidesMAGs had been identified, which could accurately classify individuals with depression from healthy individuals (AUC=0.834). Our results suggest the importance of exploring microbial markers as potential diagnostic and therapeutic targets in managing depression.
A process-based model simulating the life cycle ofCulex pipiness.s./Cx. torrentiumin Germany
Authors: Rauhöft L. , Duve P. , Martins Afonso S.M. , Jöst H. , Şuleşco T. , Sauer F.G. , Lühken R. 
Published: November 2024

AbstractMosquitoes are well known for their ability to transmit pathogens, including various arthropod-borne viruses (arboviruses) that are of veterinary and medical interest. The threat of (re-)emerging arboviruses in Europe is increasing due to globalization and climate warming. This also applies to temperate regions, where the transmission of viruses is becoming possible due to an increase of the ambient temperature, i.e. shortening of the extrinsic incubation period.Culex pipienss.s./Culex torrentiumare the primary vectors of Usutu virus and West Nile virus in Europe and is commonly found in and around human settlements. The prediction of spatial-temporal abundance allows for the early assessment of arbovirus transmission risk and the organization of intervention methods, such as vector control. A process-based model was developed to predict the spatial-temporal occurrence ofCx. pipienss.s./Culex torrentiumin Germany. The model output is driven by local rainfall and temperature data provided by the European re-analysis and observations for monitoring (E-OBS) data set. Evaluated with field data from over 123 sampling sites, the model is capable of pinpointing the peak in abundance. The mean offset of the simulated and observed peak is −3.16 (CI95=−9.73-3.4) and does not significantly differ from zero (t(145)=−0.95, p-value=0.34). One third of the sampling sites showed a significant positive Pearson correlation between simulated and observed mosquito abundance and there is an overall significant linear relationship (LM: adj. R2=0.24, F(1, 3434). The model is also capable of predicting population dynamics for entire Europe, as tested for Moldavia, Ireland, Sweden and Spain. This process-based model can be used to simulate vector control measurements or the impact of increasing temperatures in cause of climate warming.
Age-associated modulation of TREM1/2- expressing macrophages promotes melanoma progression and metastasis
Authors: Scortegagna Marzia , Murad Rabi , Bina Parinaz , Feng Yongmei , Porritt Rebecca , Terskikh Alexey , Tian Xiao , Adams Peter D. , Vuori Kristiina , Ronai Ze’ev A. 
Published: November 2024

AbstractAging is a known risk factor for melanoma, yet mechanisms underlying melanoma progression and metastasis in older populations remain largely unexplored. Among the current knowledge gaps is how aging alters phenotypes of cells in the melanoma microenvironment. Here we demonstrate that age enriches the immunosuppressor tumor microenvironment, which is linked to phenotypes associated with melanoma metastasis. Among cellular populations enriched by aging were macrophages with a tolerogenic phenotype expressing TREM2 and dysfunctional CD8-positive cells with an exhausted phenotype, while macrophages with profibrotic phenotype expressing TREM1 were depleted. Notably, TREM1 inhibition decreased melanoma growth in young but not old mice, whereas TREM2 inhibition prevented lung metastasis in aged mice. These data identify novel targets associated with melanoma metastasis and may guide aged-dependent immunotherapies.
Non-invasive visualization of pH changes within the tumor-micro-environment by positron emission tomography
Authors: Brück Jürgen , Schauenburg Dominik , Kuan Seah Ling , Göttert Simeon , Klasen Benedikt , Frommberger Veronika , Jalal Kazem Ebadi , Boui Nabil , Kwiatkowski Aaron , Schake Lisa , Bopp Tobias , Bohn Tozka , Weil Tanja , Schreckenberger Mathias , Matthias Miederer 
Published: November 2024

AbstractSlight changes in the pH value of the tumor microenvironment (TME) have crucial effects on host defense, metastatic behavior, immune regulation and cellular metabolism. Due to the high metabolic activity and insufficient perfusion of tumors, acidic metabolites often accumulate in tumors and can influence the pH of the TME. Several studies have shown that the acidity of the interstitial space and the relatively stable intracellular pH can influence the functions of cancer and stromal cells and their interaction with the extracellular matrix.We describe radiopharmaceutical probes for positron emission tomography (PET) that exploits the increasingly used concept of pH-dependent intratumorally cleavage of PET-tracer to release functional moieties. The radiopharmaceuticals are based on [18F]FDG, a PET tracer, routinely used, and substituents that are pH-dependently cleaved. Utilizing preclinical models, we were able to visualize small pH differences in the acidic TME of different tumors with [18F]FDG-4-methoxybenzylamine ([18F]FDG-4MBA).In vivostudies were in line with invitro resultsand showed that the hemiaminal bond between FDG and a substitute is cleaved at slightly acidic pH and leads to pH dependent radiotracer uptake.In vivoneutralization of the acidic extracellular tumor pH by sodium bicarbonate treatment prevents pH-dependent cleavage of [18F]FDG-4MBA and a resulting decrease of uptake. The determination of pH differences in acidic TME may serve as a novel marker for various questions such as regulation of the response to immunotherapies. Notably, even small pH differences in the acidic TME of different tumors, in the samein vivomodel, could be visualized. This is the first preclinical study to show that it is possible to visualize small pH differences in the TME of different tumors in the same mouse using hemiaminal bound [18F]FDG. Due to the facile tracer synthesis and application, this system could be well suited for translation into clinical studies to develop new strategies of pH regulation to improve the efficacy of immunotherapy in cancer patients.graphical abstract
Searching for Jouvence SnoRNA in the Horse Genome
Authors: Peffers Mandy , van den Akker Guus , Clarke Emily , Fang Yongxiang 
Published: November 2024

AbstractIn humans, various pathologies have been associated with snoRNAs. Others have shown that the expression of a non-canonical snoRNA Jouvence is involved in lifespan determination in relation to gut homeostasis. As snoRNAs are evolutionary conserved, both structurally and functionally, a jouvence orthologue has been identified in humans. This study aimed to locate the Jouvence snoRNA in the horse genome. Using our previous snoRNA data in ageing equine cartilage along with equine genome data we identified a putative equine Jouvence snoRNA gene. ECABCGRLG0000000730 was the primary candidate horse Jouvence snoRNA. The expression of equine Jouvence snoRNA was increased in ageing equine cartilage.
Pseudo-easy-axis anisotropy in antiferromagnetic
S=1
diamond-lattice systems
Authors: Vaidya S. , Hernández-Melián A. , Tidey J. P. , Curley S. P. M. , Sharma S. , Manuel P. , Wang C. , Hannaford G. L. , Blundell S. J. , Manson Z. E. , Manson J. L. , Singleton J. , Lancaster T. , Johnson R. D. , Goddard P. A. 
Published: November 2024

We investigate the magnetic properties of S=1 antiferromagnetic diamond-lattice, NiX2(pyrimidine)2 (X=Cl, Br), hosting a single-ion anisotropy (SIA) orientation which alternates between neighboring sites. Through neutron diffraction measurements of the X=Cl compound, the ordered state spins are found to align collinearly along a pseudo-easy axis, a unique direction created by the intersection of two easy planes. Similarities in the magnetization, exhibiting spin-flop transitions, and the magnetic susceptibility in the two compounds imply that the same magnetic structure and a pseudo-easy axis is also present for X=Br. We estimate the Hamiltonian parameters by combining analytical calculations and Monte Carlo (MC) simulations of the spin-flop and saturation field. The MC simulations also reveal that the spin-flop transition occurs when the applied field is parallel to the pseudo-easy axis. Contrary to conventional easy-axis systems, there exist field directions perpendicular to the pseudo-easy axis for which the magnetic saturation is approached asymptotically and no symmetry-breaking phase transition is observed at finite fields.

Published by the American Physical Society
2024
Hide and seek: the patterns, antecedents and underlying mechanisms of knowledge hiding by MNE expatriates
Authors: Zhi Ruoting , Lockett Martin , Zhou Abby Jingzi 
Published: November 2024

PurposeIn the knowledge-based view, knowledge is a valuable source of enterprise success. Its transfer through expatriates is a key mechanism in the value proposition of outward foreign direct investment by multinational enterprises (MNEs). Unfortunately, individuals are not always willing to share what they know. Expatriates are crucial players in knowledge transfer, and their knowledge-hiding behavior can significantly inhibit enterprise success. This paper thus aims to explore the patterns and antecedents of knowledge hiding, as well as the underlying mechanisms in the hiding process by expatriates within MNEs.Design/methodology/approachThis paper focuses on expatriates assigned to emerging economies in Chinese MNEs. It is based on a systematic qualitative analysis of semi-structured interviews with 24 expatriates from 16 companies across 25 countries.FindingsNot all knowledge hiding should be perceived as a negative behavior, as moderate rationalized hiding can fulfil a positive purpose. Antecedents of expatriates’ knowledge hiding were identified within a framework covering knowledge, individual, organizational and cultural features. Three main scenarios of knowledge-hiding process were also disclosed to illustrate the internal generative logic.Originality/valueThe comprehensive analysis of knowledge hiding presented in this paper enriches the existing literature on cross-border knowledge management and provides novel theoretical insights to better promote knowledge transfer by expatriates. A practical framework of knowledge transfer within MNEs is developed to guide the management of expatriates, even a broader spectrum of knowledge workers.
Balancing act: how family identity orientation impacts careers of NCAA women coaches
Authors: Boiarintseva Galina , Grabarski Mirit K. 
Published: November 2024

PurposeThe purpose of this paper is to understand the career experiences of the NCAA women coaches integrating the Kaleidoscope careers model with Family Identity theory.Design/methodology/approachWe employ qualitative methodology with the use of semi-structured interviews with 18 NCAA women coaches. Using interpretive lens, we offer nuanced descriptions of women career experiences from care and career orientation perspectives.FindingsOur findings shed light on experience, perceptions and strategies employed by female NCAA coaches while trying to fulfill work and life responsibilities. Care- or Career- orientation impacts the way women coaches deal with different aspects of maintaining careers in sports coaching.Originality/valueWe refine the KCM framework by showing that authenticity, balance and challenge can manifest in different ways, which is particularly important as organizations often struggle with retention of women coaches.
Integrating expert insights and data analytics for enhanced construction productivity monitoring and control: a machine learning approach
Authors: Pourrahimian Elyar , Eltahan Amira , Salhab Diana , Crawford Joe , AbouRizk Simaan , Hamzeh Farook 
Published: November 2024

PurposeThis study aims to enhance productivity monitoring and control in the construction industry by integrating data-driven analytics with expert insights.Design/methodology/approachA novel framework combines expert knowledge and data analysis to identify productivity trends and devise improvement strategies. A machine learning model predicts productivity ranges using historical data and project-specific factors’ evaluated by surveys, supported by a warning dashboard for proactive decision-making.FindingsThe findings reveal that integrating expert insights with data analytics significantly enhances the ability to monitor and control productivity, leading to proactive strategies for construction stakeholders. The machine learning model demonstrates robust accuracy in forecasting productivity ranges, allowing for early identification of potential issues. The dashboard system proves invaluable, offering semi-real-time alerts and facilitating swift action to prevent productivity lapses. These results highlight the effectiveness of the proposed approach in detecting trends, predicting outcomes and enabling timely interventions, thereby contributing to the overall productivity improvement of construction projects.Research limitations/implicationsThere are also limitations to consider, including potential data availability, constraints in the expert pool, implementation challenges and the need for long-term evaluation; these factors should be considered when interpreting the study’s findings and applying the proposed framework to construction projects. Future research can focus on expanding the application of this framework to different types of construction projects and evaluating its scalability.Practical implicationsThis study introduces a framework with a warning dashboard for early detection of issues, combining expert insights and data analysis for improved project outcomes. This research suggests a shift toward more expert, data-driven, insightful decision-making in construction, aiming for enhanced performance and reduced disruptions. An important implication of this research is the need to balance digital tools and expert judgment. Project managers are advised to use a holistic strategy that ensures informed and comprehensive decision-making.Originality/valueThis research introduces a unique methodology that blends traditional expertise with modern analytics to address construction productivity challenges. It offers a practical solution for stakeholders to enhance decision-making, resource allocation and project planning, marking a significant contribution to construction management literature.
Predicting project performance from occupational stress, psychological availability and ethical leadership: moderated-mediation and mediated-moderation models
Authors: Abiemo Martin Kwasi , Azila-Gbettor Edem M. , Atatsi Eli Ayawo , Honyenuga Ben Q. , Mensah Christopher 
Published: November 2024

PurposeThis study, respectively, explored the mediating and moderating influences of psychological availability and ethical leadership on the relationship between occupational stress and project performance among construction workers in Ghana.Design/methodology/approachEmploying partial least squares structural equation modelling (PLS-SEM) with SmartPLS 4, this quantitative cross-sectional study analysed survey data from 677 construction project workers who were recruited through simple random sampling technique.FindingsThe results demonstrate that occupational stress negatively predicts both project performance and psychological availability of construction project workers. Psychological availability and ethical leadership mediate and moderate the stress-project performance relationship, respectively.Practical implicationsThe findings suggest that ethical leadership and attention to psychological well-being are key to managing occupational stress and enhancing project performance in high-stress environments like construction.Originality/valueThis study contributes to the understanding of occupational stress in project management by integrating psychological and leadership factors through conservation of resource theory, social exchange theory and the job demands-resources model.