bluuebunny/crossref_metadata_2025_split huggingface dataset:
Modeling the potential range expansion of larger grain borer, Prostephanus truncatus (Coleoptera: Bostrichidae)
Authors: Arthur Frank H. , Morrison William R. , Morey Amy C. 
Published: May 2019

AbstractProstephanus truncatus (Horn) (Coleoptera: Bostrichidae), is a beetle that is a member of a family that is primarily comprised of wood-boring insects, including forest insect pests. It is native to Mexico and Central America, where it has adapted to become a pest of stored maize. It was accidentally introduced into Africa in late 1970s, where it quickly spread throughout the sub-Saharan region, perhaps aided by adaptation to alternate hosts and the ability to persist in non-agricultural habitats. We used the correlative modelling algorithm, MaxEnt, to identify global areas of potential high suitability based on the climate locations with documented populations. Predictions using a model trained in Mexico + Central America showed potential high climatic suitability extending north into the southern United States and southward into South America, including parts of Argentina, but predictions using a model built from African occurrences did not include those areas as highly suitable. However, there was general agreement in both models that large areas of the tropics in the Western Hemisphere and in Asia have climatic conditions that could support P. truncatus if it were to become established. The models also showed consistency in capturing potential suitability at sites not used to build a given model. Results can be used as an initial guide to establish surveillance programs to monitor for this insect in high risk areas where it is not currently found, and to proactively mitigate the biosecurity risk from P. truncatus.
HiBiT-qIP, HiBiT-based quantitative immunoprecipitation, facilitates the determination of antibody affinity under immunoprecipitation conditions
Authors: Ranawakage Deshani C. , Takada Takuya , Kamachi Yusuke 
Published: May 2019

AbstractThe affinity of an antibody for its antigen serves as a critical parameter for antibody evaluation. The evaluation of antibody-antigen affinity is essential for a successful antibody-based assay, particularly immunoprecipitation (IP), due to its strict dependency on antibody performance. However, the determination of antibody affinity or its quantitative determinant, the dissociation constant (Kd), under IP conditions is difficult. In the current study, we used a NanoLuc-based HiBiT system to establish a HiBiT-based quantitative immunoprecipitation (HiBiT-qIP) assay for determining the Kd of antigen-antibody interactions in solution. The HiBiT-qIP method measures the amount of immunoprecipitated proteins tagged with HiBiT in a simple yet quantitative manner. We used this method to measure the Kd values of epitope tag-antibody interactions. To accomplish this, FLAG, HA, V5, PA and Ty1 epitope tags in their monomeric, dimeric or trimeric form were fused with glutathione S-transferase (GST) and the HiBiT peptide, and these tagged GST proteins were mixed with cognate monoclonal antibodies in IP buffer for the assessment of the apparent Kd values. This HiBiT-qIP assay showed a considerable variation in the Kd values among the examined antibody clones. Additionally, the use of epitope tags in multimeric form revealed a copy number-dependent increase in the apparent affinity.
Resistin Mediates Sex-Dependent Effects of Perivascular Adipose Tissue on Vascular Function in the Shrsp
Authors: Small Heather Yvonne , McNeilly Sarah , Mary Sheon , Sheikh Adam Marcus , Delles Christian 
Published: May 2019

AbstractPremenopausal women are relatively protected from developing hypertension compared to men. Perivascular adipose tissue (PVAT) has been shown to mediate vasoactive effects; however, a sex-dependent difference in PVAT function in the setting of hypertension has not yet been explored. We investigated the effect of PVAT on resistance vessel biology in male and female 16 week old stroke prone spontaneously hypertensive rats (SHRSP). This preclinical model of hypertension exhibits a sex-dependent difference in the development of hypertension similar to humans. Wire myography was used to assess vascular function in third-order mesenteric arteries. KATPchannel-mediated vasorelaxation by cromakalim was significantly impaired in vessels from SHRSP males + PVAT relative to females (maximum relaxation: male + PVAT 46.9 ± 3.9% vs. female + PVAT 97.3 ± 2.7%). A cross-over study assessing the function of male PVAT on female vessels confirmed the reduced vasorelaxation response to cromakalim associated with male PVAT (maximum relaxation: female + PVATfemale90.6 ± 1.4% vs. female + PVATmale65.8 ± 3.5%). In order to explore the sex-dependent differences in PVAT at a molecular level, an adipokine array and subsequent western blot validation identified resistin expression to be increased approximately 2-fold in PVAT from male SHRSP vessels. Further wire myography experiments showed that pre-incubation with resistin (40 ng/ml) significantly impaired the ability of female + PVAT vessels to relax in response to cromakalim (maximum relaxation: female + PVAT 97.3 ± 0.9% vs. female + PVAT + resistin[40ng/ml]36.8 ± 2.3%). These findings indicate a novel role for resistin in mediating sex-dependent vascular function in hypertension through a KATPchannel-mediated mechanism.
Quercetin conjugated with superparamagnetic iron oxide nanoparticles improves learning and memory better than free quercetin via interacting with proteins involved in LTP
Authors: Amanzadeh Elnaz , Esmaeili Abolghasem , Abadi Rezvan Enteshari Najaf , Kazemipour Nasrin , Pahlevanneshan Zari , Beheshti Siamak 
Published: May 2019

AbstractBiomedical application of quercetin (QT) as an effective flavonoid has limitations due to its low bioavailability. Superparamagnetic iron oxide nanoparticle (SPION) is a novel drug delivery system that enhances the bioavailability of quercetin. The effect of short time usage of quercetin on learning and memory function and its signaling pathways in the healthy rat is not well understood. The aim of this study was to investigate the effect of free quercetin and in conjugation with SPION on learning and memory in healthy rats and to find quercetin target proteins involved in learning and memory using Morris water maze (MWM) and computational methods respectively. Results of MWM show an improvement in learning and memory of rats treated with either quercetin or QT-SPION. Better learning and memory functions using QT-SPION reveal increased bioavailability of quercetin. Comparative molecular docking studies show the better binding affinity of quercetin to RSK2, MSK1, CytC, Cdc42, Apaf1, FADD, CRK proteins. Quercetin in comparison to specific inhibitors of each protein also demonstrates a better QT binding affinity. This suggests that quercetin binds to proteins leading to prevent neural cell apoptosis and improves learning and memory. Therefore, SPIONs could increase the bioavailability of quercetin and by this way improve learning and memory.
App-based multidisciplinary back pain treatment versus combined physiotherapy plus online education: a randomized controlled trial
Authors: Toelle Thomas R. , Utpadel-Fischler Daniel A. , Haas Katharina-Kristina , Priebe Janosch A. 
Published: May 2019

AbstractNon-specific low back pain (LBP) is one of the leading causes of global disability. Multidisciplinary pain treatment (MPT) programs comprising educational, physical, and psychological interventions have shown positive treatment effects on LBP. Nonetheless, such programs are costly and treatment opportunities are often limited to specialized medical centers. mHealth and other digital interventions may be a promising method to successfully support patient self-management in LBP. To address these issues, we investigated the clinical effects of a multidisciplinary mHealth back pain App (Kaia App) in a randomized controlled trial (registered at German Clinical Trials Register under DRKS00016329). One-hundred one adult patients with non-specific LBP from 6 weeks to 1 year were randomly assigned to an intervention group or a control group. In the intervention group, the Kaia App was provided for 3 months. Control treatment consisted of six individual physiotherapy sessions over 6 weeks and high-quality online education. The primary outcome, pain intensity, was assessed at 12-week follow-up on an 11-point numeric rating scale (NRS). Our per-protocol analysis showed no significant differences between the groups at baseline (Kaia App group: M = 5.10 (SD = 1.07) vs. control group: M = 5.41 (SD = 1.15). At 12-week follow-up the Kaia App group reported significantly lower pain intensity (M = 2.70 (SD = 1.51)) compared to the control group (M = 3.40 (SD = 1.63)). Our results indicate that the Kaia App as a multidisciplinary back pain app is an effective treatment in LBP patients and is superior to physiotherapy in combination with online education.
Co-expression of Cas9 and single-guided RNAs in Escherichia coli streamlines production of Cas9 ribonucleoproteins
Authors: Qiao Jie , Li Wenqiang , Lin Siyu , Sun Wenli , Ma Lixin , Liu Yi 
Published: May 2019

AbstractCRISPR/Cas9 ribonucleoprotein (RNP) complexes are promising biological tools with diverse biomedical applications. However, to date there are no efficient methods that can produce these proteins at large scales and low cost. Here, we present a streamlined method for direct production of Cas9 RNPs from Escherichia coli by co-expression of Cas9 and the target-specific single-guided RNAs. Harnessing an ultrahigh-affinity CL7/Im7 purification system recently developed we achieve one-step purification of the self-assembling CRISPR/Cas RNPs, including the commonly used Cas9 and Cas12a, within half a day and with a ~fourfold higher yield than incumbent methods. The prepared Cas RNPs show remarkable stability in the absence of RNase inhibitors, as well as profound gene-editing efficiency in vitro and in vivo. Our method is convenient, cost-effective, and can be used to prepare other CRISPR/Cas RNPs.
Tutorial: Speech Motor Chaining Treatment for School-Age Children With Speech Sound Disorders
Authors: Preston Jonathan L. , Leece Megan C. , Storto Jaclyn 
Published: May 2019

Purpose
Operationalized treatments for school-age children with speech sound disorders may result in more replicable and evidence-based interventions. This tutorial describes Speech Motor Chaining (SMC) procedures, which are designed to build complex speech around core movements by incorporating several principles of motor learning. The procedures systematically manipulate factors such as feedback type and frequency, practice variability, and stimulus complexity based on the child's performance.

Method
The rationale and procedures for SMC are described. Examples are presented of how to design stimuli, deliver feedback, and adapt the approach. Free resources are provided to guide clinicians through implementation of the procedure. Data on fidelity of implementation and dose per session are presented. Clinical and research evidence is provided to illustrate likely outcomes with the procedure.

Results
SMC is a method that can result in successful acquisition of target speech patterns and generalization to untrained words. Most clinicians can implement the procedure with over 90% fidelity, and most children can achieve over 200 trials per session.

Conclusion
Clinicians and researchers can use or adapt the operationally defined SMC procedures to incorporate several principles of motor learning into treatment for school-age children with speech sound disorders.

Supplemental Material

https://osf.io/5jmf9/
Young people use their smartphone all the time – even when crossing the street?
Authors: Vollrath Mark , Huemer Anja Katharina , Nicolai Christin 
Published: May 2019

First studies from the USA indicate that distraction may also become a major health problem for pedestrians. To examine the scope of this problem for young people, an observational study was conducted in Braunschweig, Germany, looking at street crossing behaviours of students in the vicinity of four major high schools (n = 1386, 609 male, 737 children 10–14 years of age, and 649 adolescents 15–19 years). The observer recorded basic characteristics of the pedestrians (age and gender) as well as their activities before and while crossing the street (including mobile phone use). Crossing behaviour was rated with regard to safety while crossing (e.g. checking the road for cars before crossing). About 10.5% of the students used their mobile phones (looking at it and/or typing) while crossing the street. Being alone increased this to 15.3%. Talking to others lead to a reduction to 7.2%. Neither age nor gender nor the type of crossing infrastructure influenced this frequency significantly. Using the mobile phone substantially reduced safety‐related behaviours before crossing the street. Although no risky situation was directly observed, the results indicate a potential safety decrement due to mobile phone use for young pedestrians while crossing the road.
Impact of the reduced speed of light approximation on the post-overlap neutral hydrogen fraction in numerical simulations of the epoch of reionization
Authors: Ocvirk P. , Aubert D. , Chardin J. , Deparis N. , Lewis J. 
Published: May 2019

Context. The reduced speed of light approximation is used in a variety of simulations of the epoch of reionization and galaxy formation. Its popularity stems from its ability to drastically reduce the computing cost of a simulation by allowing the use of larger and therefore fewer timesteps to reach a solution. This approximation is physically motivated by the fact that ionization fronts rarely propagate faster than some fraction of the speed of light. However, no global proof of the physical validity of this approach is available and possible artefacts resulting from this approximation therefore need to be identified and characterized to allow its proper use.
Aims. In this paper we investigate the impact of the reduced speed of light approximation on the predicted properties of the intergalactic medium.
Methods. To this end we used fully coupled radiation-hydrodynamics RAMSES-CUDATON simulations of the epoch of reionization.
Results. We find that reducing the speed of light by a factor 5 (20, 100) leads to overestimating the post-reionization average volume-weighted neutral hydrogen fraction by a similar factor ∼5 (20, 100) with respect to full speed of light simulations. We show that the error is driven by the hydrogen – photon chemistry by considering the analytical solution for a strongly ionized hydrogen gas in photoionization equilibrium. In this regime, reducing the speed of light has the same effect as artificially reducing the photon density or the hydrogen photoionization cross section and leads to an underestimated ionizing intensity. We confirm this interpretation by running additional simulations using a reduced speed of light in the photon propagation module, but this time we keep the true speed of light in the chemistry module. With this set-up, the post-reionization neutral hydrogen fractions converge to the full speed of light value, which validates our explanation. Increasing spatial resolution beyond a cell size of 1 kpc physical, so as to better resolve Lyman-limit systems, does not significantly affect our conclusions.
Marguerite à Sainte-Anne : après le délire, rebroder le désir ?
Authors: Cappe Nathalie 
Published: May 2019

Marguerite Anzieu rencontra Lacan à Sainte-Anne, et celui-ci écrira sa thèse à partir de cette expérience clinique célèbre. À partir de lectures, recherche documentaires et constatations cliniques, je m’intéresse ici à un détail inexploré jusqu’ici : Marguerite à Sainte-Anne cessera d’écrire pour se consacrer à la broderie, sans que Lacan s’y intéresse vraiment. Après un résumé de certains aspects de cette histoire clinique, je m’attarderai sur cette activité manuelle. Marguerite n’a-t-elle pas trouvé dans la broderie un mode de sublimation par lequel elle a symbolisé certains aspects de sa souffrance ? Plus largement, la sublimation au féminin, du fait de certains de ses liens à la domesticité, a-t-elle plus de difficulté à se faire reconnaître ?
Global defensive k-alliances in directed graphs: combinatorial and computational issues
Authors: Mojdeh Doost Ali , Samadi Babak , Yero Ismael G. 
Published: May 2019

In this paper we define the global defensive k-alliance (number) in a digraph D, and give several bounds on this parameter with characterizations of all digraphs attaining the bounds. In particular, for the case k = −1, we give a lower (an upper) bound on this parameter for directed trees (rooted trees). Moreover, the characterization of all directed trees (rooted trees) for which the equality holds is given. Finally, we show that the problem of finding the global defensive k-alliance number of a digraph is NP-hard for any suitable non-negative value of k, and in contrast with it, we also show that finding a minimum global defensive (−1)-alliance for any rooted tree is polynomial-time solvable.
ST-Streckenhebungsinfarkt und akutes Koronarsyndrom ohne ST-Streckenhebung
Authors: Weferling Maren , Hamm Christian , Liebetrau Christoph 
Published: May 2019

ZUSAMMENFASSUNGDas akute Koronarsyndrom (ACS) umfasst den ST-Hebungsinfarkt (STEMI), den Nicht-ST-Hebungsinfarkt (NSTEMI) sowie die instabile Angina pectoris (iAP). Die beiden letztgenannten Entitäten werden als NSTE-ACS zusammengefasst. Die Europäische Gesellschaft für Kardiologie (ESC) publizierte im Jahre 2015 eine aktualisierte Version ihrer Leitlinie zum NSTEMI, sowie im Jahre 2017 zum STEMI. Zudem erfolgte in diesem Jahr die Publikation eines ESC Konsensusdokuments zur 4. universalen Definition des Myokardinfarktes. Diese Leitlinien bilden die Grundlage dieses Übersichtsartikels.
Multiple Wirbelkörperfrakturen bei einem 44-jährigen Mann – die Abklärung sekundärer Ursachen lohnt sich
Authors: Gollisch Katja S. C. , Lamersdorf Annette , Werner Mathias , Siggelkow Heide , Heppner Christina 
Published: May 2019

ZusammenfassungWir beschreiben den Fall eines 44-jährigen Patienten, der sich 2011 mit akuten Rückenschmerzen und radiologisch nachgewiesener frischer LWK-Fraktur vorstellte. Neben der frischen Fraktur fielen ältere Kompressionsfrakturen im Bereich der Brust- und Lendenwirbelsäule auf. Die Knochendichtemessung zeigte eine ausgeprägte Osteoporose. Klinisch fiel der Patient durch kleine Hoden auf. Die Diagnostik bezüglich sekundärer Ursachen der Osteoporose zeigte nicht nur einen hypergonadotropen Hypogonadismus, sondern auch das Vorliegen einer systemischen Mastozytose. Die der Osteoporose zugrundeliegenden Pathologien steuerten die Therapieentscheidung. Neben einer Testosteronsubstitution wurde eine Bisphosphonattherapie eingeleitet. Hierunter konnte eine Stabilisierung erreicht werden. Die Knochendichte verbesserte sich um 8 %, und es traten keine weiteren osteoporotischen Frakturen auf. Der Fall zeigt die Wichtigkeit einer vollständigen Diagnostik bezüglich sekundärer Ursachen einer Osteoporose, insbesondere wenn ein ungewöhnlicher Kontext wie bei jungen Männern oder prämenopausalen Frauen vorliegt.
Sorgenetzwerke am Lebensende: Interviews über das ‚gute Sterben‘ im österreichischen Bundesland Kärnten
Authors: Egger Barbara , Heimerl Katharina , Likar Rudolf , Hoppe Maria 
Published: May 2019

Zusammenfassung
Hintergrund In den letzten Jahren beobachten wir in Österreich einen lebendigen öffentlichen Diskurs zu Fragen des Ausbaus der Palliativversorgung. International erfährt die Frage nach dem erwünschten Versorgungsort große Beachtung.
Ziel Der Beitrag geht der Frage nach dem ‚guten Sterben‘ aus der Perspektive der Betroffenen im österreichischen Bundesland Kärnten nach.
Methodik Wir haben 11 qualitative, narrative und problemzentrierte Interviews mit Menschen am Lebensende und deren Angehörigen sowie eine Gruppendiskussion mit professionell Sorgenden in Kärnten durchgeführt und mittels qualitativer Inhaltsanalyse ausgewertet.
Ergebnisse Unsere Interviewpartner und -partnerinnen denken in den Gesprächen über das ‚gute Sterben‘ deutlich mehr über soziale Beziehungen als über konkrete Sterbeorte nach. Gutes Sterben bedeutet für die Betroffenen, in familiale, zivilgesellschaftliche und professionelle Sorgenetzwerke eingebettet zu sein.
Schlussfolgerung Im Sinne der Betroffenen unterstützen Caring Communities die Entwicklung von familialen, zivilgesellschaftlichen und professionellen Sorgenetzwerken.
The phonological structure of Japanese mimetics and motherese
Authors: Kubozono Haruo 
Published: May 2019

Japanese is known to have a rich inventory of words both in mimetics and motherese (baby talk words). The purpose of this chapter is to examine interesting similarities that these two types of vocabulary exhibit in phonological structure. First of all, words in both mimetics and motherese are basically disyllabic although mimetic words also permit longer strings of syllables. Second, they are subject to a prosodic restriction by which word-final sequences of a light syllable plus a heavy one are prohibited. Third, all baby talk words are lexically ‘accented’, i.e., pronounced with an abrupt pitch fall, as opposed to ‘unaccented’. This prosodic feature is also shared by mimetic words. This fact is astonishing because a majority of native words in adult Japanese are lexically unaccented, which raises the interesting question of why mimetics and motherese are accentually different from the adult language.
By carefully analyzing various phenomena in adult phonology, this chapter presents evidence that the phonological biases commonly shown by mimetics and motherese represent unmarked phonological structures of the language that emerge in derived words in adult phonology. This allows us to link mimetics and motherese on the one hand, and adult and child phonology, on the other.
The Gender of Subscribers to Eighteenth-Century Music Publications
Authors: Fleming Simon D. I. 
Published: May 2019

One of the most important and valuable resources available to researchers of eighteenth-century social history are the lists of subscribers that were attached to a wide variety of publications. Yet, the study of this type of resource remains one of the areas most neglected by academics. These lists shed considerable light on the nature of those who subscribed to music, including their social status, place of employment, residence, and musical interests. They naturally also provide details as to the gender of individual subscribers.As expected, subscribers to most musical publications were male, but the situation changed considerably as the century progressed, with more females subscribing to the latest works by the early nineteenth century. There was also a marked difference in the proportion of male and female subscribers between works issued in the capital cities of London and Edinburgh and those written for different genres. Female subscribers also appear on lists to works that they would not ordinarily be permitted to play. Ultimately, a broad analysis of a large number of subscription lists not only provides a greater insight into the social and economic changes that took place in Britain over the course of the eighteenth century, but also reveals the types of music that were favoured by the members of each gender.