bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nucleic Acid Therapeutics in Huntington’s Disease
Authors: Singh Kuljit , Roy Ipsita 
Published: February 2019

Background:
Protein misfolding is a critical factor in the progression of a large number
of neurodegenerative diseases. The incorrectly folded protein is prone to aggregation, leading to
aberrant interaction with other cellular proteins, elevated oxidative stress, impaired cellular machinery,
finally resulting in cell death. Due to its monogenic origin, Huntington’s disease (HD) is
a poster child of protein misfolding neurodegenerative disorders. The presence of neuronal inclusions
of mutant huntingtin N-terminal fragments, mainly in the cortex and striatum, is a neuropathological
hallmark of HD. Inhibition of protein misfolding and aggregation has been attempted
using a variety of conventional protein stabilizers.

Methods:
This review describes how, in recent times, nucleic acid therapeutics has emerged as a
selective tool to downregulate the aberrant transcript and reduce expression of mutant huntingtin,
thereby alleviating protein aggregation. Different strategies of use of nucleic acids, including antisense
oligonucleotides, short inhibitory RNA sequences and aptamers have been discussed. The
following patent databases were consulted: European Patent Office (EPO), the United States Patent
and Trademark Office (USPTO), Patent scope Search International and National Patent Collections
(WIPO) and Google Patents.

Results:
Tools such as RNA interference (RNAi) and antisense oligonucleotides (ASOs) are potential
therapeutic agents which target the post-transcriptional step, accelerating mRNA degradation
and inhibiting the production of the mutant protein. These nucleic acid sequences not only
target the elongated CAG triplet repeat translating to an expanded polyglutamine tract in the mutant
protein, but have also been used to target single nucleotide polymorphisms associated with the
mutant allele. The therapeutic sequences have been investigated in a number of cells and animal
models of HD. One antisense sequence, with desirable safety properties, has recently shown
downregulation of huntingtin protein in a limited clinical trial. RNA aptamers have also shown
promising results in inhibiting protein aggregation in a yeast model of HD. Novel drug delivery
techniques have been employed to overcome the blood brain barrier for the use of these therapeutic
sequences.

Conclusion:
The selectivity and specificity imparted by nucleic acids, along with novel delivery
techniques, make them hopeful candidates for the development of a curative strategy for HD.
Potential Angiotensin Converting Enzyme Inhibitors from Moringa oleifera
Authors: Khan Huma , Jaiswal Varun , Kulshreshtha Saurabh , Khan Azhar 
Published: February 2019

Background:
Hypertension is the chronic medical condition and it affected billions of
people worldwide. Natural medicines are the main alternatives to treatment for a majority of people
suffering from hypertension. Niazicin-A, Niazimin-A, and Niaziminin-B compounds from
Moringa oleifera ethanolic leave extract were reported to have potent antihypertensive activity.

Objective:
These compounds were targeted with Angiotensin-converting enzyme [ACE] which is
one of the main regulatory enzymes of the renin-angiotensin system.

Methods:
Protein-ligand docking of these compounds with [ACE] [both domain N and C] was
conceded out through Autodock vina and visualization was done by chimera. Pharmacokinetics
study of these compounds was predicted by ADME-Toxicity Prediction.

Results:
Niazicin-A, Niazimin-A, and Niaziminin-B showed high binding affinity with ACE and
partially blocked the active sites of the enzyme. Niazicin-A, Niazimin-A and Niaziminin-B
showed the estimated free binding energy of -7.6kcal/mol kcal/mol, -8.8kcal/mol and -8.0kcal/mol
respectively with C-domain of ACE and -7.9kcal/mol, -8.5kcal/mol and -7.7kcal/mol respectively
with N-domain of ACE. The compounds showed better binding energy with angiotensinconverting
enzyme in comparison to Captopril -5.5kcal/mol and -5.6kcal/mol and Enalapril [standard]
-8.4kcal/mol and -7.5kcal/mol with C and N domain, respectively.

Conclusion:
Computationally, the selected bioactive molecules have shown better binding energy
to known standard drugs which have been already known for inhibition of ACE and can further
act as a pharmacophore for in vitro and in vivo studies in the development of alternative medicine.
Network Lifetime and Throughput Analysis in Wireless Sensor Networks Using Fuzzy Logic
Authors: Kumar Hradesh , Singh Pradeep K. 
Published: February 2019

Background:
Currently, Wireless sensor networks are the most prominent area in research.
Energy consumption is one of the important challenges in wireless sensor networks.

Objective:
The objective of this paper is to increase the network lifetime and throughput of the
wireless sensor network.

Methods:
The fuzzy logic approach is used to improve the network lifetime and throughput. The
proposed approach gave better results in comparison to existing approaches of Low Energy
Fuzzy Based Unequal Clustering Multi-hop Architecture (LEFUCMA) and Low Energy Adaptive
Unequal Clustering Using Fuzzy C-Means (LAUCF).

Results:
The proposed approach is 11.39 % better in terms of network lifetime in comparison to
LEFUCMA and 34.27 % in terms of network lifetime in comparison to LAUCF.

Conclusion:
The proposed approach is 34.29 % better in terms of network throughput as a comparison
of LEFUCMA and 112.85 % in terms of network throughput in comparison to LAUCF.
Ersatz Capitalism in Indonesian Automotive Industry during The New Order Era, 1969—1998
Authors: Anugrah Insan Praditya 
Published: February 2019

This paper examines a historical trajectory using Kunio Yoshihara’s concept of ersatz capitalism to analyze the development of Indonesian automotive Industry during New Order Era, 1969-1998. The concept analyzes the fact that the ersatz capitalism structure and ersatz capitalists behavior had caused the failure of Indonesian automotive Industry development to achieve self-reliance. Ersatz Capitalism is characterized by patronage between capitalists and bureaucrats, the capitalists reluctance to invest in research and technological development, foreign capital and foreign technology domination, and the domestic capitalists role that act only as a distributor of the manufactured high-technology products of foreign principals instead of doing research and development, in order to seek immediate high profit return with minimum investment. Through the research, this paper analyzes the role of the ersatz capitalists from the various background such as military, Chinese capitalists, and president families and relatives had supported foreign principal domination In Indonesia. Those capitalists are reluctant to support government programs toward self-reliance industry and choose to strengthen their ties with foreign principals, in order to get immediate profit return without much investment and technological research.
Metastatic Breast Cancer Presenting as Refractory Anemia
Authors: Beaudoin-Maitre Julie , Vallée Chantal 
Published: February 2019

We will review the case of a 71-year-old woman with an atypical presentation of stage IV breast cancer. She presented with symptomatic normocytic anemia (Hb 85 to 100 g/L) for the past two years, with a normal extended laboratory evaluation. Due to deterioration of her anemia, a bone marrow biopsy was done and concluded to myelodysplastic syndrome. Treatment with Epoetin alfa and a trial of Lenalidomide didn’t improve her hemoglobin and she was still transfusion dependent. A second bone marrow biopsy revealed breast carcinoma infiltration, which was initially missed on the first biopsy. The patient was started on Letrozole and Palbociclib with good clinical response. Here, we present the clinical evolution, diagnosis and management of bone marrow micrometastasis due to breast cancer.

Résumé
Nous présentons l’étude de cas d’un cancer du sein de stade IV, chez une femme de 71 ans, ayant eu une présentation atypique. Elle s’est présentée avec une anémie normocytaire (Hb 85 à 100 g/L) symptomatique depuis deux ans, non explicable par un bilan biochimique approfondi. Suite à la dégradation de son anémie, une biopsie de moelle osseuse a été effectuée concluant à un syndrome myélodysplasique. Un traitement à l’epoetin alfa ainsi qu’un essai de lenalidomide ne permirent pas l’amélioration de son hémoglobine et elle est demeurée dépendante aux transfusions. Une seconde biopsie de moelle osseuse démontra une infiltration par un carcinome mammaire qui n’avait pas été constatée lors de la première biopsie. Un traitement avec du letrozole et du palbociclib a alors été débuté avec une réponse clinique satisfaisante. Nous passons en revue la présentation clinique, la démarche diagnostique ainsi que la prise en charge des micro métastases de la moelle osseuse dans le cancer du sein.
PUBLICIDADE, ADAPTAÇÃO E TRANSMÍDIA: A COMPOSIÇÃO DA NARRATIVA DO CINEMA INFANTIL BRASILEIRO NO FINAL DA DÉCADA DE 1980
Authors: Bona Rafael José 
Published: February 2019

O artigo tem como objetivo analisar uma obra infantil cinematográfica nacional, representativa do
perfil dos filmes do final da década de 1980: O casamento dos Trapalhões (1988, José Alvarenga Júnior). Os
objetivos específicos são: identificar as referências do filme sob a ótica das transposições/adaptações; analisar a
publicidade inserida nesse filme; e identificar elementos transmídia na obra. Parte-se do pressuposto que muitos
dos filmes do antigo grupo humorístico Os Trapalhões, além de introduzirem os jovens espectadores ao contato
com outras obras, também praticavam um exercício publicitário, e se utilizavam de técnicas de transmídia e
adaptação. A obra é analisada, principalmente, por meio de conceitos dos autores Seger (2007), Jenkins (2009) e
Hutcheon (2013). Como principal resultado, constata-se que a obra faz uma adaptação alusiva a um filme dos
EUA dos anos de 1950 (Sete noivas para sete irmãos, dir. Stanley Donen, 1954), insere a publicidade de forma
(in)discreta no contexto narrativo e sua principal técnica transmídia está relacionada ao nexo com os personagens
da televisão e dos quadrinhos do grupo Os Trapalhões.
As Noivas Vermelhas
Authors: Marquetti Fernanda Cristina 
Published: February 2019

Resumo
Este ensaio discute o suicídio feminino a partir das relações de gênero. Nós abordamos o suicídio feminino em duas culturas, China e Brasil. A partir das conexões deste evento nas interações culturais feminino-masculino surgiram diferentes estratégias de desqualificação do feminino que estavam relacionadas ao suicídio, sejam estas, estratégias discursivas ou comportamentais. Assim, podemos considerar que o suicídio feminino é um evento social e intrinsicamente relacionado a questão da identidade da mulher nas duas culturas.
Palavras chaves: Suicídio. Gênero. Cultura.
Misoginia no traço: violência contra as mulheres como pretexto temático em charges
Authors: N. Volpato Alana , A. Damião Nayara , A. Miani Rozinaldo 
Published: February 2019

Este artigo pretende refletir sobre o uso da violência contra mulheres como um pretexto temático utilizado como argumento visual na produção de charges. Entendemos que as charges consistem em uma modalidade do humor gráfico, produzido pelo traço humano, de natureza dissertativa. A charge, imbuída de crítica política e social, é construída retratando um determinado ponto de vista sobre um fato histórico por meio de recursos visuais. Produzida no contexto da sociedade patriarcal, pode reproduzir as relações de dominação e exploração a que as mulheres estão submetidas. Por meio da metodologia de análise do discurso chárgico, analisamos a presença de mulheres em charges que discutem temas como política, economia e sociedade, enfatizando o uso da violência contra a mulher - sobretudo, violência sexual - como um pretexto temático para construir a crítica na charge, que acaba contribuindo para a manutenção de estereótipos e da condição opressiva imposta sobre as mulheres.
Palavras-chave: Violência contra a mulher. Patriarcado. Charge. Humor gráfico.
Psicodelia, humor e militância: os coletivos de mulheres quadrinistas no comix underground norte americano
Authors: Medeiros Talita S 
Published: February 2019

Atualmente muitas mulheres vem se destacando como autoras de histórias em quadrinhos, embora ainda em número menor do que em relação aos homens, podemos observar trabalhos bastante significativos e cada vez mais autoras produzindo. Isso se dá em especial pelas redes de apoio construídas por elas, estratégia utilizada desde há muito tempo para forjar seus próprios espaços de publicação. Neste artigo perpassaremos por algumas das primeiras estratégias de inserção das autoras de quadrinhos. Em especial, quando no bojo da contracultura norte-americana surgem os primeiros coletivos de mulheres quadrinistas: as obras It Aint' Me Babe (1970) e Wimmenscomix (1972-1992), Tits&Clits (1972-1987). Observaremos como através da articulação em revistas produzidas apenas por mulheres, foi possível que houvesse uma organização enquanto quadrinistas, uma abertura de espaços para as futuras autoras e uma produção de obras originais – os comix underground pela perspectiva feminina.
Palavras-chave: Quadrinistas. Coletivos. Comix. Feminismo. Arte. Contracultura.
Dona Marcolina, uma megera indomada: representações e estereótipos de feminilidade nos quadrinhos de Alceu Chichôrro
Authors: Mannala Thaís , Queluz Marilda L. P. 
Published: February 2019

Neste artigo, pretendemos refletir sobre a personagem Dona Marcolina, criada pelo jornalista e chargista Alceu Chichôrro (1896-1977), publicada no jornal curitibano O Dia, no período de 1926 a 1961. Na companhia de Chico Fumaça e do cachorro Totó, Dona Marcolina representa um tipo de feminilidade associado às mulheres, constituído de camadas de discursos normatizantes e hierarquizantes, com traços físicos caricatos, como também modos exagerados de agir, pensar e reagir a determinadas práticas sociais masculinas, ironizando os espaços de domínio das mulheres e questionando quem, de fato, deveria mandar na casa. Dentro da tradição do humor gráfico e das chamadas family strips do início do século XX, destacam-se personagens femininas – caracterizadas por serem tiranas, mandonas, mal-humoradas e fora dos padrões de beleza da época – que geralmente são descritas como feias. Além disso, são desenhadas altas, magras, em contraponto a seus pares, homens baixinhos e gordos. Estes contrastes e desproporções provocam o efeito cômico, evocando a comédia popular, e reiteram a inversão de papéis sociais esperados pela sociedade. Estas personagens mandam, controlam e batem nos homens. O pau/rolo de macarrão torna-se um artefato comum no desfecho das charges e tiras cômicas, usado no último quadrinho para golpear e punir os deslizes, travessuras dos homens.
Palavras-chave: Representações. Estereótipos. Gênero. Tiras cômicas. Dona Marcolina. Alceu Chichôrro.
Client Deadline Concentration in Audit Offices and Audit Quality
Authors: Czerney Keith , Jang Daun , Omer Thomas C. 
Published: February 2019

SUMMARY
This research investigates the effect on audit quality of concentrated public company financial statement filing deadlines in audit offices. Audit offices must effectively manage their resources to meet clients' audit service requirements. When an audit office has deadlines that are more concentrated in time, effective resource management is of greater importance to reduce the likelihood of audit failure. Drawing on relevant research from the auditing and management literatures, we hypothesize and find that audit quality is lower when an audit office's clients' financial statement deadlines concentrate in time, which we term client deadline concentration. The significant, negative effect of client deadline concentration on audit quality is incremental to the effects of other resource-based constraints from the prior literature and to controls for unobservable differences in audit offices that explain a significant amount of the variation in audit quality outcomes.
JEL Classifications: M40; M41; M42; M48.
Data Availability: Data are available from public sources identified in the text.
Consensus Statement on Public Involvement and Engagement with Data-Intensive Health Research
Authors: Aitken Mhairi , Tully Mary P , Porteous Carol , Denegri Simon , Cunningham-Burley Sarah , Banner Natalie , Black Corri , Burgess Michael , Cross Lynsey , Van Delden Johannes , Ford Elizabeth , Fox Sarah , Fitzpatrick Natalie , Gallacher Kay , Goddard Catharine , Hassan Lamiece , Jamieson Ron , Jones Kerina H , Kaarakainen Minna , Lugg-Widger Fiona , McGrail Kimberlyn , McKenzie Anne , Moran Rosalyn , Murtagh Madeleine J , Oswald Malcolm , Paprica Alison , Perrin Nicola , Richards Emma Victoria , Rouse John , Webb Joanne , Willison Donald J 
Published: February 2019

This consensus statement reflects the deliberations of an international group of stakeholders with a range of expertise in public involvement and engagement (PI&E) relating to data-intensive health research. It sets out eight key principles to establish a secure role for PI&E in and with the research community internationally and ensure best practice in its execution. Our aim is to promote culture change and societal benefits through ensuring a socially responsible trajectory for innovations in this field.
Our key premise is that the public should not be characterised as a problem to be overcome but a key part of the solution to establish socially beneficial data-intensive health research for all.
Furto no contexto escolar | Theft in schools
Authors: Moraes Couto Leandra Lúcia , Alencar Heloisa Moulin de 
Published: February 2019

Pesquisadoras ligadas à Universidade Federal do Espírito Santo, do Laboratório de Psicologia da Moralidade (Lapsim), Leandra Lúcia Moraes Couto e Heloisa Moulin de Alencar, identificaram que profissionais da educação básica não possuem clareza sobre as práticas pedagógicas que devem adotar em situações de furto no contexto escolar. As estudiosas constataram também que tais profissionais elegem ações para as situações de furto que podem interferir negativamente no desenvolvimento dos alunos, pois envolvem a humilhação, a imposição e/ou o constrangimento. Como exemplo, as autoras citam os castigos, a averiguação do material do aluno, entre outros. Há profissionais que têm a percepção de que práticas como essas são injustas. Mas, consideram que a tentativa de resolver o problema de furto no contexto escolar justifica a prática. Couto e Alencar discutem que esse tipo de resultado chama a atenção para a necessidade de se investir na formação de profissionais da educação, para que eles se sintam seguros para atuar nas diversas situações que envolvem a violência no contexto escolar, bem como adquiram os conhecimentos necessários para a promoção do desenvolvimento moral dos educandos.
SELECTION OF SOYBEAN CULTIVARS: PREPARATION THE PLANT BREEDING WITH MICROSPORE CULTURE METHOD
Authors: Sumarmi Sumarmi 
Published: February 2019

Microspore culture method can be used as plant breeding program. The preparation of cultivars selection is an important step. The research starts with cultivated five cultivars of soybean i.e: Argomulyo, Grobogan, Wilis, Anjasmoro and Black Malika. The appearence of soybean plant was observed until flowering. Selection of plants based on: sum of flower bud every plant, anther midline, total and diameter of microspore every flower bud was measured by the ‘Optilab’ software. The development of microspore done with grouping of flower bud according long 2.02.5 mm, 2.6-3.0 mm, 3.1- 3.6 mm and 3.7-4.1 mm for chooses flower bud with the most late uninucleate microspore stadium. Result of the research shows that long of flower bud 2.6-3.6 mm contain 1847-2010 late uninucleate microspores, diameter 20 µm for 5 cultivars can be used for material of microspore culture. Anjasmoro cultivar, tall of plant gain 68 cm, sum of rame 7-9, anther midline 354.67±59.67 µm, number of microspores each flower bud 2003±216. Result of responsive qulity test with anther incubation on 340C temperature for 4 days represent the most of total viable microspore, 3.371±45 on Anjasmoro cultivar. Plant breeding by Anjasmoro cultivar is the most appropriate for microspore culture treatment.
Work-Related Psychosocial Risk Factors and Care Workers Mental Health (In Estonian Nursing Homes)
Authors: Sepp Jaana , Järvis Marina , Reinhold Karin 
Published: February 2019

Abstract
The humankind is ageing rapidly, and as a result, there is an increasing need for old people’s homes. The nursing homes face different problems in financing and recruiting the labour force and management. Lack of resources causes the situation, when managers have to find possibilities to accomplish services and to provide quality care with the limited funds. This situation has an additional impact on the nursing professionals, who have to deal with many psychosocial risk factors in their work. The aim of the paper is to explore the work-related psychosocial risk factors and their relationships with mental health problems (MHPs) amongst care workers. A cross-sectional survey was undertaken amongst the care workers in nine Estonian nursing homes. Psychosocial work factors and MHPs (stress, somatic symptoms, depressive symptoms, burnout, cognitive symptoms, and sleep disorders) were analysed using the second version of the Copenhagen Psychosocial Questionnaire (COPSOQ II). Descriptive statistics and Pearson’s r correlation were used to analyse the data. The analysis was based on 340 care worker surveys. The highest mean scores for the studied work-related psychosocial factors were recorded for the quantitative demands, influence, rewards, role conflicts, trust, insecurity and work-family balance. Low mean scores were recorded for the meaning of work, role clarity, social relationships at work. The lowest score was followed by burnout and the highest - by cognitive symptoms.
The Analysis of the Digital AC Motor Speed Control System Operation
Authors: Sznajder Joanna 
Published: February 2019

Abstract
The article presents the analysis of the automatic alternate current motor control system, carried out by the author. The automatic control system has been implemented on the existing laboratory stand, containing: the squirrel-cage asynchronous motor and the frequency inverter. The existing stand imposed one of the available speed control methods for the motor and the necessity of the appropriate elements selection for the automatic control system [1]. The automatic control system has been designed and created as the constant value follow-up digital controller. To designate the parameters of the control object the unit step method of was used. After registering the output changes caused by the unit step, the characteristic curve was received that allowed to determine the alternative transmittance of the control object which, in turn, has made possible to find the appropriate controller settings.
Energy Storage System with the use of Supercapacitors
Authors: Tarczyński Wiesław , Kopka Ryszard 
Published: February 2019

Abstract
The article presents an analysis of performance of an energy storage element used in uninterruptible power supply systems built with the use of supercapacitors. It describes the operation of the voltage balancing system during charging and discharging of the capacitors. Attention has been drawn to the energy conditions of the system's operation and solutions that provide enhanced energy efficiency have been described.