bluuebunny/crossref_metadata_2025_split huggingface dataset:
Interferon-Tau Exerts Direct Prosurvival and Antiapoptotic Actions in Luteinized Bovine Granulosa Cells
Authors: Basavaraja Raghavendra , Madusanka Senasige Thilina , Drum Jessica N. , Shrestha Ketan , Farberov Svetlana , Wiltbank Milo C. , Sartori Roberto , Meidan Rina 
Published: October 2019

AbstractInterferon-tau (IFNT), serves as a signal to maintain the corpus luteum (CL) during early pregnancy in domestic ruminants. We investigated here whether IFNT directly affects the function of luteinized bovine granulosa cells (LGCs), a model for large-luteal cells. Recombinant ovine IFNT (roIFNT) induced the IFN-stimulated genes (ISGs; MX2, ISG15, and OAS1Y). IFNT induced a rapid and transient (15–45 min) phosphorylation of STAT1, while total STAT1 protein was higher only after 24 h. IFNT treatment elevated viable LGCs numbers and decreased dead/apoptotic cell counts. Consistent with these effects on cell viability, IFNT upregulated cell survival proteins (MCL1, BCL-xL, and XIAP) and reduced the levels of gamma-H2AX, cleaved caspase-3, and thrombospondin-2 (THBS2) implicated in apoptosis. Notably, IFNT reversed the actions of THBS1 on cell viability, XIAP, and cleaved caspase-3. Furthermore, roIFNT stimulated proangiogenic genes, including FGF2, PDGFB, and PDGFAR. Corroborating the in vitro observations, CL collected from day 18 pregnant cows comprised higher ISGs together with elevated FGF2, PDGFB, and XIAP, compared with CL derived from day 18 cyclic cows. This study reveals that IFNT activates diverse pathways in LGCs, promoting survival and blood vessel stabilization while suppressing cell death signals. These mechanisms might contribute to CL maintenance during early pregnancy.
Measurement of the hysteretic thermal properties of W-doped and undoped nanocrystalline powders of VO2
Authors: Gomez-Heredia C. L. , Ramirez-Rincon J. A. , Bhardwaj D. , Rajasekar P. , Tadeo I. J. , Cervantes-Lopez J. L. , Ordonez-Miranda J. , Ares O. , Umarji A. M. , Drevillon J. , Joulain K. , Ezzahri Y. , Alvarado-Gil J. J. 
Published: October 2019

AbstractHysteresis loops exhibited by the thermal properties of undoped and 0.8 at.% W-doped nanocrystalline powders of VO2 synthesized by means of the solution combustion method and compacted in pellets, are experimentally measured by photothermal radiometry. It is shown that: (i) the W doping reduces both the hysteresis loops of VO2 and its transition temperature up to 15 °C. (ii) The thermal diffusivity decreases (increases) until (after) the metallic domains become dominant in the VO2 insulating matrix, such that its variation across the metal-insulation transition is enhanced by 23.5% with W-0.8 at.% doping. By contrast, thermal conductivity (thermal effusivity) increases up to 45% (40%) as the metallic phase emerges in the VO2 structure due to the insulator-to-metal transition, and it enhances up to 11% (25%) in the insulator state when the local rutile phase is induced by the tungsten doping. (iii) The characteristic peak of the VO2 specific heat capacity is observed in both heating and cooling processes, such that the phase transition of the 0.8 at.% W-doped sample requires about 24% less thermal energy than the undoped one. (iv) The impact of the W doping on the four above-mentioned thermal properties of VO2 mainly shows up in its insulator phase, as a result of the distortion of the local lattice induced by the electrons of tungsten. W doping at 0.8 at.% thus enhances the VO2 capability to transport heat but diminishes its thermal switching efficiency.
Superoxide dismutase down-regulation and the oxidative stress is required to initiate pupation in Bombyx mori
Authors: Nojima Yosui , Bono Hidemasa , Yokoyama Takeshi , Iwabuchi Kikuo , Sato Ryoichi , Arai Katsuhiko , Tabunoki Hiroko 
Published: October 2019

AbstractPerhaps, oxidative stress progresses pupation in some Lepidopteran insects; however, the reasons for this remain obscure. In our previous study, we clarifiedBombyx moriSOD1 (BmSOD1) andB. moriSOD2 (BmSOD2) proteins respond in common to ultraviolet irradiation (UV) oxidative stress and metamorphosis. This result strongly suggested pupation initiates by oxidative stress and might mediate by down-regulation of expression of BmSOD1 and BmSOD2 proteins. Thus, we examined about these relationships inB. moriin this study. In the microarray data reanalysis, we found the Notch signaling pathways as the common pathways in pupation and UV oxidative stress inB. mori. Also, we showed a molting hormone, 20-hydroxyecdysone, leads not only generation of superoxide but also downregulation of the expression of BmSOD proteins during pupation inB. mori. Our findings can contribute to a deeper understanding of how biological defense systems work against environmental oxidative stress.
Small molecule FAK activator promotes human intestinal epithelial monolayer wound closure and mouse ulcer healing
Authors: Wang Qinggang , More Shyam K. , Vomhof-DeKrey Emilie E. , Golovko Mikhail Y. , Basson Marc D. 
Published: October 2019

AbstractGI mucosal healing requires epithelial sheet migration. The non-receptor tyrosine kinase focal adhesion kinase (FAK) stimulates epithelial motility. A virtual screen identified the small drug-like FAK mimic ZINC40099027, which activates FAK. We assessed whether ZINC40099027 promotes FAK-Tyr-397 phosphorylation and wound healing in Caco-2 monolayers and two mouse intestinal injury models. Murine small bowel ulcers were generated by topical serosal acetic acid or subcutaneous indomethacin in C57BL/6J mice. One day later, we began treatment with ZINC40099027 or DMSO, staining the mucosa for phosphorylated FAK and Ki-67 and measuring mucosal ulcer area, serum creatinine, ALT, and body weight at day 4. ZINC40099027 (10–1000 nM) dose-dependently activated FAK phosphorylation, without activating Pyk2-Tyr-402 or Src-Tyr-419. ZINC40099027 did not stimulate proliferation, and stimulated wound closure independently of proliferation. The FAK inhibitor PF-573228 prevented ZINC40099027-stimulated wound closure. In both mouse ulcer models, ZINC40099027accelerated mucosal wound healing. FAK phosphorylation was increased in jejunal epithelium at the ulcer edge, and Ki-67 staining was unchanged in jejunal mucosa. ZINC40099027 serum concentration at sacrifice resembled the effective concentration in vitro. Weight, creatinine and ALT did not differ between groups. Small molecule FAK activators can specifically promote epithelial restitution and mucosal healing and may be useful to treat gut mucosal injury.
Patterns of at-sea behaviour at a hybrid zone between two threatened seabirds
Authors: Austin Rhiannon E. , Wynn Russell B. , Votier Stephen C. , Trueman Clive , McMinn Miguel , Rodríguez Ana , Suberg Lavinia , Maurice Louise , Newton Jason , Genovart Meritxell , Péron Clara , Grémillet David , Guilford Tim 
Published: October 2019

AbstractPatterns of behavioural variation and migratory connectivity are important characteristics of populations, particularly at the edges of species distributions, where processes involved in influencing evolutionary trajectories, such as divergence, mutual persistence, and natural hybridization, can occur. Here, we focused on two closely related seabird species that breed in the Mediterranean: Balearic shearwaters (Puffinus mauretanicus) and Yelkouan shearwaters (Puffinus yelkouan). Genetic and phenotypic evidence of hybridization between the two species on Menorca (the eastern and westernmost island in the breeding ranges of the two shearwaters, respectively) has provided important insights into relationships between these recently diverged species. Nevertheless, levels of behavioural and ecological differentiation amongst these populations remain largely unknown. Using geolocation and stable isotopes, we compared the at-sea movement behaviour of birds from the Menorcan ‘hybrid’ population with the nearest neighbouring populations of Balearic and Yelkouan shearwaters. The Menorcan population displayed a suite of behavioural features intermediate to those seen in the two species (including migration strategies, breeding season movements and limited data on phenology). Our findings provide new evidence to support suggestions that the Menorcan population is admixed, and indicate a role of non-breeding behaviours in the evolutionary trajectories ofPuffinusshearwaters in the Mediterranean.
Do conservation strategies that increase tiger populations have consequences for other wild carnivores like leopards?
Authors: Kumar Ujjwal , Awasthi Neha , Qureshi Qamar , Jhala Yadvendradev 
Published: October 2019

AbstractMost large carnivore populations are declining across their global range except in some well managed protected areas (PA’s). Investments for conserving charismatic apex carnivores are often justified due to their umbrella effect on biodiversity. We evaluate population trends of two large sympatric carnivores, the tiger and leopard through spatially-explicit-capture-recapture models from camera trap data in Kanha PA, India, from 2011 to 2016. Our results show that the overall density (100 km−2) of tigers ranged between 4.82 ± 0.33 to 5.21 ± 0.55SE and of leopards between 6.63 ± 0.71 to 8.64 ± 0.75SE, with no detectable trends at the PA scale. When evaluated at the catchment scale, Banjar catchment that had higher prey density and higher conservation investments, recorded significant growth of both carnivores. While Halon catchment, that had lower prey and conservation investments, populations of both carnivores remained stable. Sex ratio of both carnivores was female biased. As is typical with large carnivores, movement parameter sigma (an index for range size), was larger for males than for females. However, sigma was surprisingly similar for the same genders in both carnivores. At home-range scale, leopards achieved high densities and positive growth rates in areas that had low, medium or declining tiger density. Our results suggest that umbrella-species conservation value of tigers is likely to be compromised at very high densities and therefore should not be artificially inflated through targeted management.
Increased plasmatic NETs by-products in patients in severe obesity
Authors: D’Abbondanza Marco , Martorelli Eva Edvige , Ricci Maria Anastasia , De Vuono Stefano , Migliola Elisa Nulli , Godino Cosmo , Corradetti Sara , Siepi Donatella , Paganelli Maria Teresa , Maugeri Norma , Lupattelli Graziana 
Published: October 2019

AbstractNeutrophil extracellular traps (NETs) are DNAs products involved in immune process. Obesity through a low-grade chronic inflammation determines neutrophil activation, but it is still unclear its role in NETs formation. Here we analyzed the NETs levels in healthy and morbid obese, their association with anthropometric and glyco-metabolic parameters and their changes after bariatric surgery. For this study, we enrolled 73 patients with morbid obesity (BMI ≥40 kg/m2 or ≥35 kg/m2 + comorbidity) eligible to sleeve gastrectomy. In parallel, 55 healthy subjects and 21 patients with severe coronary artery disease were studied as controls. We evaluated anthropometric parameters, peripheral blood pressure, biochemical and serum analysis at the enrollment and at twelve months after surgery. Plasmatic levels of MPO-DNA complexes were assessed by ELISA. NETs levels were higher in obese than in control group (p < 0.001) and correlated with the main anthropometric variable (BMI, waist, hip), glyco-metabolic variables and systolic blood pressure. NETs trend after intervention was uneven. The reduction of NETs correlated with the entity of reduction of BMI (ρ = 0.416, p < 0.05), visceral fat area (ρ = 0.351, p < 0.05), and glycemia (ρ = 0.495, p < 0.001). In medical history of patients in whom NETs increased, we observed a higher number of thromboembolic events. Our observations indicate that severe obesity is associated with increased generation of NETs, which in turn could influence the patients’ systemic inflammatory state. Weight loss and in particular, loss of adipose tissue after bariatric surgery does not in itself correct NET’s dysregulated production. Finally, patients in whom NETs accumulation persists after surgery are probably those at the highest risk of cardiovascular events.
Environmental factors and river network position allow prediction of benthic community assemblies: A model of nematode metacommunities
Authors: Gansfort Birgit , Traunspurger Walter 
Published: October 2019

AbstractThe field of metacommunity studies is growing rapidly, including recent applications to river networks. Most of these studies have targeted a single river network but whether their findings are relevant to other river systems is unknown. This study investigated the influence of environmental, spatial and temporal parameters on the community structure of nematodes in the river networks of the Elbe and Rhine. We asked whether the variance in community structure was better explained by spatial variables representing the watercourse than by overland distances. After determining the patterns in the Elbe river network, we tested whether they also explained the Rhine data. The Elbe data were evaluated using a boosted regression tree analysis. The predictive ability of the model was then assessed using the Rhine data. In addition to strong temporal dynamics, environmental factors were more important than spatial factors in structuring riverine nematode communities. Community structure was more strongly influenced by watercourse than by Euclidean distances. Application of the model’s predictions to the Rhine data correlated significantly with field observations. Our model shows that the consequences of changes in environmental factors or habitat connectivity for aquatic communities across different river networks are quantifiable.
Increased interactions and engulfment of dendrites by microglia precede Purkinje cell degeneration in a mouse model of Niemann Pick Type-C
Authors: Kavetsky Larisa , Green Kayla K. , Boyle Bridget R. , Yousufzai Fawad A. K. , Padron Zachary M. , Melli Sierra E. , Kuhnel Victoria L. , Jackson Harriet M. , Blanco Rosa E. , Howell Gareth R. , Soto Ileana 
Published: October 2019

AbstractNiemann Pick Type-C disease (NPC) is an inherited lysosomal storage disease (LSD) caused by pathogenic variants in theNpc1orNpc2genes that lead to the accumulation of cholesterol and lipids in lysosomes. NPC1 deficiency causes neurodegeneration, dementia and early death. Cerebellar Purkinje cells (PCs) are particularly hypersensitive to NPC1 deficiency and degenerate earlier than other neurons in the brain. Activation of microglia is an important contributor to PCs degeneration in NPC. However, the mechanisms by which activated microglia promote PCs degeneration in NPC are not completely understood. Here, we are demonstrating that in theNpc1nmf164mouse cerebellum, microglia in the molecular layer (ML) are activated and contacting dendrites at early stages of NPC, when no loss of PCs is detected. During the progression of PCs degeneration inNpc1nmf164mice, accumulation of phagosomes and autofluorescent material in microglia at the ML coincided with the degeneration of dendrites and PCs. FeedingNpc1nmf164mice a western diet (WD) increased microglia activation and corresponded with a more extensive degeneration of dendrites but not PC somata. Together our data suggest that microglia contribute to the degeneration of PCs by interacting, engulfing and phagocytosing their dendrites while the cell somata are still present.
Remodeling of macular vortex veins in pachychoroid neovasculopathy
Authors: Matsumoto Hidetaka , Kishi Shoji , Mukai Ryo , Akiyama Hideo 
Published: October 2019

AbstractSuperior and inferior macular vortex veins are divided by a horizontal watershed passing through the macula. We evaluated macular vortex vein remodeling in eyes with pachychoroid neovasculopathy (PNV). Thirty eyes of 30 patients with treatment-naïve PNV and 30 normal eyes of 30 age-, gender-, and refraction-matched subjects were studied. We assessed the features of macular vortex veins employing en face optical coherence tomography (OCT) and determined central choroidal thickness (CCT) using B-mode OCT. Of the 30 normal eyes, a horizontal watershed was identified in 24 eyes (80%), while venous anastomosis between the superior and inferior vortex veins was observed in 6 eyes (20%). Mean CCT was 233 μm. Of the 30 eyes with PNV, vortex veins were dilated in all 30 eyes with PNV. In 27 of the 30 PNV eyes (90%), the horizontal watershed had disappeared, and collateral veins had instead developed via anastomosis between the superior and inferior vortex veins, making this finding significantly more frequent than in normal eyes (P < 0.001). Mean CCT was 357 μm, significantly thicker than that of normal eyes (P < 0.001). Remodeling of choroidal drainage routes by venous anastomosis between superior and inferior vortex veins was common in eyes with PNV. This observation suggests longstanding congestion of the choroidal veins.
Urinary myo-inositol is associated with the clinical outcome in focal segmental glomerulosclerosis
Authors: An Jung Nam , Hyeon Jin Seong , Jung Youngae , Choi Young Wook , Kim Jin Hyuk , Yang Seung Hee , Oh Sohee , Kwon Soie , Lee Sang-Ho , Cho Jang-Hee , Park Sun-Hee , Ha Hunjoo , Kim Dong Ki , Lee Jung Pyo , Hwang Geum-Sook 
Published: October 2019

AbstractFocal segmental glomerulosclerosis (FSGS) and minimal change disease (MCD) have similar initial histological findings; however, their prognoses are distinct. Therefore, it is of great importance to discriminate FSGS from MCD in the early phase of disease and predict clinical prognosis. A discovery set of 184 urine samples (61 healthy control, 80 MCD, and 43 FSGS) and a validation set of 61 urine samples (12 healthy control, 26 MCD, and 23 FSGS) were collected at the time of kidney biopsy. Metabolic profiles were examined using nuclear magnetic resonance spectroscopy. Of 70 urinary metabolites, myo-inositol was significantly higher in FSGS patients than in control patients (discovery set, 2.34-fold, P < 0.001; validation set, 2.35-fold, P = 0.008) and MCD patients (discovery set, 2.48-fold, P = 0.002; validation set, 1.69-fold, P = 0.042). Myo-inositol showed an inverse relationship with the initial estimated glomerular filtration rate (eGFR) and was associated with the plasma level of soluble urokinase-type plasminogen activator receptor in FSGS patients. Myo-inositol treatment ameliorated the decreased expression of ZO-1 and synaptopodin in an in vitro FSGS model, and as myo-inositol increased, myo-inositol oxygenase tissue expression decreased proportionally to eGFR. Furthermore, urinary myo-inositol exhibited an increase in the power to discriminate FSGS patients, and its addition could better predict the response to initial treatment. In conclusion, urinary myo-inositol may be an important indicator in the diagnosis and treatment of FSGS patients.
Keystone-design perforator island flaps for the management of complicated epidermoid cysts on the back
Authors: Yoon Chi Sun , Kim Hyo Bong , Kim Young Keun , Kim Hoon , Kim Kyu Nam 
Published: October 2019

AbstractComplicated epidermoid cysts (ECs) occur commonly on the back, but few reports have described their management. We present our experience in managing patients with ECs on the back using a keystone-design perforator island flap (KDPIF) reconstruction, thereby focusing on reduction and redistribution of wound tension. Altogether, 15 patients (average age, 48.067 ± 14.868 years) underwent KDPIF reconstructions after complete excision of complicated ECs on the back. We retrospectively reviewed the medical records and clinical photographs of all patients. Final scar appearance was evaluated using the Patient and Observer Scar Assessment Scale (POSAS). All patients had ruptured ECs, while 6 patients also had cellulitis of the surrounding tissues. All defects, after complete excision of ECs and debridement of surrounding unhealthy tissues, were successfully covered with KDPIF. The mean ‘tension-change’ at the defect and donor sites was −4.73 ± 0.21 N and −4.88 ± 0.25 N, respectively (p < 0.001). The mean ‘rate of tension-change’ at the defect and donor sites was −69.48 ± 1.7% and −71.16 ± 1.33%, respectively (p < 0.001). All flaps survived with no postoperative complications. The mean observer scar assessment scale (OSAS) summary score and patient scar assessment scale (PSAS) total score were 14.467 ± 5.069 and 15.6 ± 6.512, respectively. Overall, we suggest that KDPIF reconstruction is a good surgical modality for the management of complicated ECs on the back.
Beneficial effects of Lactobacillus reuteri 6475 on bone density in male mice is dependent on lymphocytes
Authors: Collins Fraser L. , Rios-Arce Naiomy Deliz , Schepper Jonathan D. , Jones A. Daniel , Schaefer Laura , Britton Robert A. , McCabe Laura R. , Parameswaran Narayanan 
Published: October 2019

AbstractOral treatment with probiotic bacteria has been shown to prevent bone loss in multiple models of osteoporosis. In previous studies we demonstrated that oral administration of Lactobacillus reuteri in healthy male mice increases bone density. The host and bacterial mechanisms of these effects however are not well understood. The objective of this study was to understand the role of lymphocytes in mediating the beneficial effects of L. reuteri on bone health in male mice. We administered L. reuteri in drinking water for 4 weeks to wild type or Rag knockout (lack mature T and B lymphocytes) male mice. While L. reuteri treatment increased bone density in wild type, no significant increases were seen in Rag knockout mice, suggesting that lymphocytes are critical for mediating the beneficial effects of L. reuteri on bone density. To understand the effect of L. reuteri on lymphocytes in the intestinal tissues, we isolated mesenteric lymph node (MLN) from naïve wild type mice. In ex vivo studies using whole mesenteric lymph node (MLN) as well as CD3+ T-cells, we demonstrate that live L. reuteri and its secreted factors have concentration-dependent effects on the expression of cytokines, including anti-inflammatory cytokine IL-10. Fractionation studies identified that the active component of L. reuteri is likely water soluble and small in size (<3 kDa) and its effects on lymphocytes are negatively regulated by a RIP2 inhibitor, suggesting a role for NOD signaling. Finally, we show that T-cells from MLNs treated with L. reuteri supernatants, secrete factors that enhance osterix (transcription factor involved in osteoblast differentiation) expression in MC3T3-E1 osteoblasts. Together, these data suggest that L. reuteri secreted factors regulate T-lymphocytes which play an important role in mediating the beneficial effects of L. reuteri on bone density.