bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hypothesis: Wound‐induced TLR3 activation stimulates endogenous retinoic acid synthesis and signalling during regeneration
Authors: Kim Dongwon , Garza Luis A. 
Published: March 2019

AbstractAlthough the mechanism is unclear, it has been shown that genetically normal adult mice with a large wound form de novo morphogenesis of hair follicles in wound‐induced hair neogenesis (WIHN)(1). We focused on how tissues recognize damage signals and identified that double‐stranded RNA (dsRNA)‐mediated toll‐like receptor 3 (TLR3) activation stimulates WIHN. Here, we propose a hypothesis that TLR3 stimulates retinoic acid synthesis and signalling to allow for regeneration, suggesting that common clinical methods of facial rejuvenation in human subjects through damage (such as lasers or dermabrasion), and the use of topical retinoids reflect the same biologic pathway.
A high‐throughput screening for inhibitors of riboflavin synthase identifies novel antimicrobial compounds to treat brucellosis
Authors: Serer María Inés , Carrica Mariela del Carmen , Trappe Jörg , López Romero Sandra , Bonomi Hernán Ruy , Klinke Sebastián , Cerutti María Laura , Goldbaum Fernando Alberto 
Published: March 2019

Brucella spp. are pathogenic intracellular Gram‐negative bacteria adapted to life within cells of several mammals, including humans. These bacteria are the causative agent of brucellosis, one of the zoonotic infections with the highest incidence in the world and for which a human vaccine is still unavailable. Current therapeutic treatments against brucellosis are based on the combination of two or more antibiotics for prolonged periods, which may lead to antibiotic resistance in the population. Riboflavin (vitamin B2) is biosynthesized by microorganisms and plants but mammals, including humans, must obtain it from dietary sources. Owing to the absence of the riboflavin biosynthetic enzymes in animals, this pathway is nowadays regarded as a rich resource of targets for the development of new antimicrobial agents. In this work, we describe a high‐throughput screening approach to identify inhibitors of the enzymatic activity of riboflavin synthase, the last enzyme in this pathway. We also provide evidence for their subsequent validation as potential drug candidates in an in vitro brucellosis infection model. From an initial set of 44 000 highly diverse low molecular weight compounds with drug‐like properties, we were able to identify ten molecules with 50% inhibitory concentrations in the low micromolar range. Further Brucella culture and intramacrophagic replication experiments showed that the most effective bactericidal compounds share a 2‐Phenylamidazo[2,1‐b][1,3]benzothiazole chemical scaffold. Altogether, these findings set up the basis for the subsequent lead optimization process and represent a promising advancement in the pursuit of novel and effective antimicrobial compounds against brucellosis.
Daylength helps temperate deciduous trees to leaf‐out at the optimal time
Authors: Fu Yongshuo H. , Zhang Xuan , Piao Shilong , Hao Fanghua , Geng Xiaojun , Vitasse Yann , Zohner Constantin , Peñuelas Josep , Janssens Ivan A. 
Published: March 2019

AbstractGlobal warming has led to substantially earlier spring leaf‐out in temperate‐zone deciduous trees. The interactive effects of temperature and daylength underlying this warming response remain unclear. However, they need to be accurately represented by earth system models to improve projections of the carbon and energy balances of temperate forests and the associated feedbacks to the Earth's climate system. We studied the control of leaf‐out by daylength and temperature using data from six tree species across 2,377 European phenological network (www.pep725.eu), each with at least 30 years of observations. We found that, in addition to and independent of the known effect of chilling, daylength correlates negatively with the heat requirement for leaf‐out in all studied species. In warm springs when leaf‐out is early, days are short and the heat requirement is higher than in an average spring, which mitigates the warming‐induced advancement of leaf‐out and protects the tree against precocious leaf‐out and the associated risks of late frosts. In contrast, longer‐than‐average daylength (in cold springs when leaf‐out is late) reduces the heat requirement for leaf‐out, ensuring that trees do not leaf‐out too late and miss out on large amounts of solar energy. These results provide the first large‐scale empirical evidence of a widespread daylength effect on the temperature sensitivity of leaf‐out phenology in temperate deciduous trees.
Effects of ultrasound‐assisted frying on the physiochemical properties and microstructure of fried meatballs
Authors: Wang Yan , Zhang Wangang , Zhou Guanghong 
Published: March 2019

SummaryThe aim of this study was to investigate the effects of power ultrasound (0, 200, 400, 600 and 800 W, 20 kHz) on the quality of fried meatballs. The frying time and frying temperature were also considered as fixed factors. The meatballs were fried for 8, 12 and 16 min at 120, 140 and 160 °C. Results showed that the ultrasonic groups saved about 1 to 3 min than the control group to 80 °C. Hardness, springiness, cohesiveness and chewiness of fried meatballs were significantly different among different ultrasonic treatments (P < 0.05). As for colour, the ultrasonic treatments could significantly increase the L*values. Cooking yield was from 82.58% to 85.50% in ultrasonic treatments at 120 °C for 8 min. High moisture retention and cooking yield were shown in fried‐assisted ultrasound treatments and were consistent with the microstructure of scanning electron microscopy (SEM). Our data show that ultrasound‐assisted frying could improve the quality of meatballs.
Validation of the International Tumor Budding Consensus Conference (2016) recommendations in oral tongue squamous cell carcinoma
Authors: Xie Nan , Yu Pei , Liu Haichao , Liu Xiqiang , Hou Jinsong , Chen Xiaohua , Huang Hongzhang , Wang Cheng 
Published: March 2019

AbstractBackgroundTumor budding is a valuable prognostic marker in oral tongue squamous cell carcinoma (OTSCC) but lacks a standardized scoring system. The aim of this study was to evaluate the prognostic value of tumor budding for OTSCC patients based on the scoring system recommended by the International Tumor Budding Consensus Conference (ITBCC) 2016.MethodsTumor budding was scored as ITBCC recommended in 255 patients with OTSCC. Then, associations between tumor budding and clinicopathologic parameters were examined. Among them, 136 patients with follow‐up data available were used to evaluate overall survival (OS) by the Kaplan‐Meier method. Prognostic value of tumor budding was assessed by Cox regression analysis. The inter‐observer and intra‐observer agreement was calculated by the kappa statistic.ResultsTumor budding score was associated with lymph node metastasis, differentiation, invasive pattern, lymphoid infiltrate, tumor relapse, invasive depth, and reduced OS in OTSCC patients. The Cox analysis showed high budding score was an independent prognostic factor in patients with all clinical stage and patients with clinical early‐stage OTSCC. The high kappa values were achieved in intra‐observer and inter‐observer.ConclusionsInternational Tumor Budding Consensus Conference scoring system is a simple, reliable, and reproducible method to measure tumor budding in OTSCC, which should be included in the routine pathological report.
A revealed preference index of urban amenities: Using travel demand as a proxy
Authors: Broxterman Daniel A. , Kuang Chun 
Published: March 2019

AbstractWe introduce an index of urban amenities based on hotel night stays that can rank most US metros for the years 1990 through 2015. This naive index can be used on its own to study changes in amenity level or with other measures to reduce omitted variable bias. Researchers can also use the index to create panel datasets from more sophisticated cross‐sectional indexes. Empirical testing shows the bundle of amenities associated with hotel stays has substantial effects on variation in housing prices across cities and time, validating our proxy variable approach. Testing for an association with wages is inconclusive.
Cosmic ray exposure ages for ureilites—New data and a literature study
Authors: Leya Ingo , Stephenson Peter C. 
Published: March 2019

AbstractWe report newly measured noble gas isotopic concentrations of He, Ne, and Ar for 21 samples from the 10 ureilites, DaG 084, DaG 319, DaG 340, Dho 132, HaH 126, JaH 422, JaH 424, Kenna, NWA 5928, and RaS 247, including the results of both single and stepwise heating extractions. Cosmic ray exposure (CRE) ages calculated using model calculations that fully account for all shielding depths and a wide range of preatmospheric radii, and are tailored to ureilite chemistry, range from 3.7 Ma for Dho 132 to 36.3 Ma for one of several measured Kenna samples. In a Ne‐three‐isotope plot, the data for DaG 340 and JaH 422 plot below the Necos/Neureilite mixing envelope, possibly indicating the presence of Ne produced from solar cosmic rays. In combination with literature data and correcting for pairing, we established a fully consistent database containing 100 samples from 40 different ureilites. The CRE age histogram shows a trend of decreasing meteorite number with increasing CRE age. We speculate that the parent body of the known ureilites is moving closer to a resonance and/or that there is a loss mechanism that acts on ureilites independent of their size. In addition, there is a slight indication for a peak in the range 30 Ma, which might indicate a larger impact on the ureilite daughter body. Finally, we confirm earlier results that the majority of the studied ureilites have relatively small preatmospheric radii less or equal ~20 cm.
Sodium‐powered stators of the bacterial flagellar motor can generate torque in the presence of phenamil with mutations near the peptidoglycan‐binding region
Authors: Ishida Tsubasa , Ito Rie , Clark Jessica , Matzke Nicholas J. , Sowa Yoshiyuki , Baker Matthew A. B. 
Published: March 2019

SummaryThe bacterial flagellar motor powers the rotation that propels the swimming bacteria. Rotational torque is generated by harnessing the flow of ions through ion channels known as stators which couple the energy from the ion gradient across the inner membrane to rotation of the rotor. Here, we used error‐prone PCR to introduce single point mutations into the sodium‐powered Vibrio alginolyticus/Escherichia coli chimeric stator PotB and selected for motors that exhibited motility in the presence of the sodium‐channel inhibitor phenamil. We found single mutations that enable motility under phenamil occurred at two sites: (i) the transmembrane domain of PotB, corresponding to the TM region of the PomB stator from V. alginolyticus and (ii) near the peptidoglycan binding region that corresponds to the C‐terminal region of the MotB stator from E. coli. Single cell rotation assays confirmed that individual flagellar motors could rotate in up to 100 µM phenamil. Using phylogenetic logistic regression, we found correlation between natural residue variation and ion source at positions corresponding to PotB F22Y, but not at other sites. Our results demonstrate that it is not only the pore region of the stator that moderates motility in the presence of ion‐channel blockers.
Association between delayed recall and T2* relaxation time of the subiculum in adolescents: Implications for ultra‐high‐field magnetic resonance imaging
Authors: Jeon Sehyun , Hwang Seok‐Il , Son Young Don , Kim Young‐Bo , Lee Yu Jin , Kim Seog Ju 
Published: March 2019

AimThe aim of this study was to assess neuropsychological correlations with the T2* relaxation time (T2*‐RT) of hippocampal subregions in adolescents using ultra‐high‐field (UHF) 7.0‐T magnetic resonance imaging (MRI).MethodsWe assessed the T2*‐RT of hippocampal subregions in 31 healthy 11th‐ or 12th‐grade high school students using an UHF 7.0‐T MRI system. T2*‐RT of the cornu ammonis (CA) 1, CA2, CA3, and CA4 subregions and the subiculum were calculated for both the left and right hippocampus. Seven subtests of the Cambridge Neuropsychological Test Automated Battery were administered to the subjects to assess visuospatial memory.ResultsPoor performances in delayed recall in the pattern‐recognition test were significantly correlated with longer T2*‐RT in the bilateral subiculum (right, r = −0.480, P = 0.006; left, r = −0.648, P < 0.001) and the left CA2 (r = −0.480, P = 0.006).ConclusionThis study showed that longer T2*‐RT in the subiculum were associated with poorer performances in delayed recall in the visual memory tasks. This finding suggests that the subiculum might play a predominant role in delayed recall in adolescents.
Cyanine Modification Tuned for Amine Photorelease
Authors: Mathew Joel P. , Greer Alexander 
Published: March 2019

AbstractCyanines are emerging as useful agents for photoreleasing biological compounds because of their capability of utilizing near‐infrared (NIR) light. Another benefit is their ability to self‐sensitize to produce singlet oxygen for the release of aryl amines, a process that has not been as feasible in the past. Here, we highlight the paper by Schnermann et al. (https://doi.org/10.1111/php.13090), which reports on a cyanine conjugate for heterolytic photocleavage of aryl amines. This paper is timely—delving into a photorelease mechanism involving a domino rearrangement and β‐elimination triggered by NIR light.
Nightmares do result in psychophysiological arousal: A multimeasure ambulatory assessment study
Authors: Paul Franc , Alpers Georg W. , Reinhard Iris , Schredl Michael 
Published: March 2019

AbstractIndividuals who frequently experience nightmares report compromised sleep quality, poor daytime mood, and functioning. Previous research has aimed at linking these impairments with altered sleep architecture, but results were inconclusive. One plausible explanation is that only a few studies recorded markers of autonomic nervous system activity. For the first time, this study collected such markers under ecologically valid conditions with ambulatory assessment. In 19 individuals with frequent nightmares (≥1 nightmare/week) and 19 healthy control participants (<1 nightmare/month), measures indicating autonomic activation (heart rate, heart rate variability, respiration cycle length, electrodermal fluctuations, EEG arousals, saliva cortisol, REM density) were collected while applying ambulatory polysomnographic assessment during 3 consecutive nights. When nightmare participants reported a nightmare, we analyzed the last 5 min of REM sleep before awakening and compared these data to their non‐nightmares as well as to the dream episodes of control participants. Overall, there were no general differences in autonomic activation of nightmare sufferers compared to control participants. However, when nightmare participants experienced nightmares, autonomic activation was markedly increased compared to their own non‐nightmares and, to some extent, to control participant’s dreams. Significant intraindividual differences were found for all autonomic measures except in participant’s EEG arousals and cortisol levels. Group differences were found in EEG arousals and heart rate. In conclusion, ambulatory polysomnography demonstrates that nightmares are accompanied by increased autonomic activation. Results support the notion of impaired self‐reported sleep quality caused by one’s autonomic response rather than altered sleep pattern.
The promise of bioimpedance for volume management in American dialysis patients: An unfulfilled opportunity
Authors: Kotanko Peter 
Published: March 2019

AbstractEvidence from both observational and randomized controlled studies indicates that the use of bioimpedance in the care of chronic hemodialysis patients is associated with improved outcomes, in particular better volume and blood pressure control. Bioimpedance as a means to assess fluid status in dialysis patients has been approved by numerous regulatory agencies and is being used in dozens of countries around the globe. The most notable exception to the worldwide acceptance of this technique is the US where no BIA device has been approved by the US Food and Drug Administration for use in dialysis patients. The reasons for this unfortunate situation are manifold and mostly flawed. They include the view that bioimpedance is inaccurate and not sufficiently validated, that clinical judgment is good enough to assess fluid status, that interdialytic changes in weight are satisfactory indicators of fluid status (overload, depletion, and euvolemia), costs, and regulatory and marketing barriers. Easing the path to approval and modifying the dialysis payment structure are necessary to bring dialysis care closer to the levels that will optimize patient outcomes.
Out of Africa? A dated molecular phylogeny of the cicada tribe Platypleurini Schmidt (Hemiptera: Cicadidae), with a focus on African genera and the genusPlatypleuraAmyot & Audinet‐Serville
Authors: Price Benjamin W. , Marshall David C. , Barker Nigel P. , Simon Chris , Villet Martin H. 
Published: March 2019

AbstractThe Platypleurini is a large group of charismatic cicadas distributed from Cape Agulhas in South Africa, through tropical Africa, Madagascar, India and eastern Asia to Japan, with generic diversity concentrated in equatorial and southern Africa. This distribution suggests the possibility of a Gondwanan origin and dispersal to eastern Asia from Africa or India. We used a four‐gene (three mitochondrial) molecular dataset, fossil calibrations and molecular clock information to explore the phylogenetic relationships of the platypleurine cicadas and the timing and geography of their diversification. The earliest splits in the tribe were found to separate forest genera in Madagascar and equatorial Africa from the main radiation, and all of the Asian/Indian species sampled formed a younger clade nested well within the African taxa. The tribe appears to have diversified during the Cenozoic, beginningc. 50–32 Ma, with most extant African lineages originating in the Miocene or later, well after the breakup of the Gondwanan landmass. Biogeographical analysis suggests an African origin for the tribe and a single dispersal event founding the Asian platypleurines, although additional taxon sampling and genetic data will be needed to confirm this pattern because key nodes in the tree are still weakly supported. Two Platypleurini genera from Madagascar (PycnaAmyot & Audinet‐Serville,YangaDistant) are found to have originated by late Miocene dispersal of a single lineage from Africa. The genusPlatypleurais recovered as polyphyletic, withPlatypleura signiferaWalker from South Africa and many Asian/Indian species apparently requiring assignment to different genera, and a newPlatypleuraconcept is proposed with the synonymization ofAzanicadaVilletsyn.n.The generaOrapaDistant andHamzaDistant, currently listed within separate tribes but suspected of platypleurine affinity, are nested deeply within the Platypleurini radiation. The tribe Orapinisyn.n. is here synonymized while the tribe Hamzini is pending a decision of the ICZN to preserve nomenclatorial stability.
Identification of a novel HLA‐DPB1, HLA‐DPB1*687:01, in an Italian renal transplant candidate
Authors: Biagini Chiara , Fornaciari Silvia , Mariotti Maria L. , Mazzoni Alessandro , Curcio Michele 
Published: March 2019

AbstractTwo adjacent nucleotide substitutions in exon 2 characterize the novel class II HLA‐DPB1*687:01 allele.
Hybrid single‐port laparoscopic cisterna chyli ablation for the adjunct treatment of chylothorax disease in dogs
Authors: Morris Katherine P. , Singh Ameet , Holt David E. , Stefanovski Darko , Singhal Sunil , Bosco Jessica , Capps Michelle , McCallum Michael , Runge Jeffrey J. 
Published: March 2019

AbstractObjectiveTo describe a hybrid, single‐port, minimally invasive cisterna chyli ablation (CCA) technique in clinical cases of canine idiopathic chylothorax and evaluate this technique as a method for mesenteric lymphangiography (ML) in canine cadavers and clinical cases of idiopathic chylothorax.Study designCadaveric and retrospective study.AnimalsSix canine cadavers and 14 client‐owned dogs with naturally occurring idiopathic chylothorax.MethodsBoth cadaveric and clinically affected dogs were placed in sternal recumbency. A wound retractor device (WRD) and a single‐port device were placed in the abdominal flank 2–3 cm caudal to the 13th rib. Mesenteric lymphangiography was evaluated by using indocyanine green (ICG) in 6 canine cadavers. Single‐port laparoscopic CCA was performed in all clinical cases with idiopathic chylothorax.ResultsSuccessful ML was completed by using ICG in all 6 canine cadavers. A right‐ or left‐sided single‐port laparoscopic CCA was successfully performed in 14 dogs with naturally occurring idiopathic chylothorax. Mesenteric lymphangiography was successfully performed through the WRD in 11 of these cases. No intraoperative complications were reported. Three dogs developed severe chyloabdomen postoperatively, with 1 dog requiring multiple abdominocenteses.ConclusionDirect ML and single‐port laparoscopic CCA was performed through a WRD in dogs positioned in sternal recumbency. Although minimal operative complications were noted, postoperative chyloabdomen was reported.Clinical significanceThis hybrid single‐port laparoscopic technique performed in sternal recumbency allows both a CCA and an intraoperative ML through the same incision. This procedure may be combined with thoracoscopic thoracic duct ligation and pericardectomy for the treatment of idiopathic chylothorax in dogs.
Haemostasis treatment using dual red imaging during endoscopic submucosal dissection: a multicentre, open-label, randomised controlled trial
Authors: Fujimoto Ai , Saito Yutaka , Abe Seiirhicro , Hoteya Shu , Nomura Kosuke , Yasuda Hiroshi , Matsuo Yasumasa , Uraoka Toshio , Kuribayashi Shiko , Saito Itaru , Tsuji Yosuke , Maehata Tadateru , Ochiai Yasutoshi , Nishizawa Toshihiro , Yahagi Naohisa 
Published: March 2019

IntroductionPatients scheduled to undergo oesophageal, gastric and colorectal endoscopic submucosal dissection (ESD) are to be investigated to verify the efficacy of dual red imaging (DRI) for establishing haemostasis during ESD.Methods and analysisThe trial is designed as a multicentre, open-label randomised, parallel-group, controlled intervention study. Registered patients will be randomly assigned to DRI and white light imaging (WLI) groups. In the DRI group, the mucosal incision and submucosal dissection will be performed by WLI, and haemostasis will be managed by DRI when bleeding occurs. In the WLI group, the mucosal incision and submucosal dissection are to be performed by WLI and the haemostasis management is to be performed by WLI. The primary endpoint is the time from the recognition of bleeding up to the achievement of complete haemostasis (haemostasis time). The secondary endpoints are the operation time, the proportion of cases in which perforation occurs, and the psychological stress experienced by the endoscopist during haemostasis treatment.Ethics and disseminationThis trial was approved by the Keio University Review Board for Clinical Trials (5 December 2016).DiscussionThis will be the first multicentre collaborative research using DRI for haemostasis treatment during ESD. When the safety and simplicity of DRI as a treatment for haemostasis during ESD can be proven, the ESD procedure can be simplified and disseminated more widely in clinical practice.Trial registration numberUMIN000025134