bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mild Affective Shifts and Creativity: Effects on Idea Generation, Evaluation, and Implementation Planning
Authors: Watts Logan L. , McIntosh Tristan J. , Gibson Carter , Mulhearn Tyler J. , Medeiros Kelsey E. , Mecca Jensen T. , Cohen‐Charash Yochi 
Published: October 2019

AbstractThis study investigated the impact of mild shifts in affective tone (i.e., pleasant vs. unpleasant) and arousal (i.e., high vs. low) on three creative processes. Undergraduates read short stories designed to induce affective shifts and then were asked to generate solutions to a complex business problem. Shifts in affective tone and arousal interacted to influence idea generation and implementation planning, but not idea evaluation. The strongest creative performance was exhibited by participants who experienced a stable and pleasantly toned, low‐arousal (i.e., relaxed) state and by those who shifted to an unpleasantly toned, high‐arousal (i.e., angry) state. In contrast, those who shifted to an unpleasantly toned, low‐arousal (i.e., sad) state or a pleasantly toned, high‐arousal (i.e., happy) state tended to exhibit poorer creative performance. These results demonstrate the need to revisit the popular conception that organizations should promote intense levels of pleasant affect to enhance employee creativity.
Weight gain velocity as a predictor of severe obstructive sleep apnea among obese adolescents
Authors: Johnson Romaine F. , Hansen Alexander , Narayanan Ajay , Yogesh Ahana , Shah Gopi B. , Mitchell Ron B. 
Published: October 2019

Objectives/HypothesisTo evaluate a cohort of obese adolescents with obstructive sleep apnea (OSA) to determine if increased yearly weight gain was a predictor of severe OSA.Study DesignRetrospective cohort study.MethodsObese adolescents (body mass index percentile >95% for that age and sex based upon the Centers for Disease Control and Prevention weight classifications), ages 12 to 17 years, referred for full night polysomnography (PSG) were analyzed. We examined demographics, weight classifications, yearly weight gain from age 9 years onward, PSG data (apnea‐hypopnea index), and tonsil size. We performed a mixed‐effect linear regression model to test whether the velocity of weight gain was increased in obese patients with severe OSA when compared to those without severe OSA.ResultsThis study included 166 obese adolescents, 105 with and 61 without severe OSA. The average age was 14 years and was predominately male (57%) and Hispanic (44%). The regression analysis found that the yearly change in weight among obese adolescents with severe OSA was significantly higher than those without (B = 1.4, standard error = 0.50, P = .005, 95% confidence interval: 0.42–2.4). For the group with severe OSA, weight increased 6.5 kg every year before their PSG, whereas for those without, weight increased 5.1 kg per year.ConclusionsThe rate of weight gain over time is an important predictor of severe OSA in obese adolescents.Level of Evidence3bLaryngoscope, 130:1339–1342, 2020
High stream intermittency in an alpine fluvial network: Val Roseg, Switzerland
Authors: Paillex Amael , Siebers Andre R. , Ebi Christian , Mesman Jorrit , Robinson Christopher T. 
Published: October 2019

AbstractMore than one‐third of the world's rivers cease to flow and go dry on a periodic basis—so‐called intermittent rivers. The frequency and duration of flow intermittency in running waters are increasing due to climate change and water demands for human use. Intermittency effects on stream biodiversity and ecosystem functioning are dramatic and are expected to become increasingly prevalent in alpine landscapes in the near future. This project used modified field sensors to measure flow intermittency, temperature, and water origin (groundwater, precipitation, glacier) at high spatio‐temporal resolution throughout an alpine fluvial network (Val Roseg, Switzerland). We continuously recorded water presence in 30 tributary streams and validated sensor performance with field‐collected measures. Three different flow regimes were observed in the network, including periodically intermittent, seasonally intermittent, and permanently flowing streams. Twenty‐four streams (80% of recorded streams) dried at least once during the sampling period. Principal components analysis along with generalized additive models showed alpine streams with low average temperature and high conductivity (groundwater‐fed) were prone to permanent flow, whereas streams with higher average temperature and low conductivity (glacier‐fed) typically had intermittent flow. The field sensors proved precise for simultaneously measuring flow intermittency, temperature, and water origin at high resolution throughout the river network. Overall, this approach provides an effective way to develop eco‐hydrological models that examine the effects of flow intermittency on biodiversity and ecosystem functioning in riverine networks.
The role of large macrofauna in mediating sediment erodibility across sedimentary habitats
Authors: Hillman Jenny R. , Lundquist Carolyn J. , Pilditch Conrad A. , Thrush Simon F. 
Published: October 2019

AbstractLarge macrofauna influence sediment erodibility via their activity and presence in the sediment. This article explores how the depletion of large animals from macrobenthic communities influences sediment erodibility across a natural grain size gradient of poorly sorted habitats within an estuary. We sampled seven sites in Mahurangi Harbour, New Zealand, to investigate how heterogeneity within and among these habitats influences sediment stability. We depleted the large macrofauna in the sediment to determine how changes in community structure will affect ecosystem functioning in the context of sediment stability. A core‐based erosion device (EROMES) was used to measure three different parameters associated with sediment resuspension potential: surface erosion threshold (τ
c; N m−2); erosion rate (ER; g m−2 s−1); and the subsurface erosion constant (m
e; g N−1 s−1). Multiple regression models were developed for each parameter to identify important drivers of change. Sediment grain size, as a proxy for habitat type, explained 53% of the total variation in both the early surface erosion measures, τ
c and ER. Once the surface layers had been eroded, m
e was best defined by a site‐specific combination of biological, chemical, and physical variables that explained 40% of the subsurface erosion. Our results demonstrate that at all sites reducing the abundance of large animals within the macrofaunal community contributed to substantial impacts on erosion and thus ecosystem functioning associated with sediment composition and water clarity.
Calcium carbonate production by fish in temperate marine environments
Authors: Salter Michael A. , Perry Chris T. , Smith Abigail M. 
Published: October 2019

AbstractMarine bony fishes are an important source of calcium carbonate with relevance to sediment production and inorganic carbon cycling. However, knowledge of the production and fate of these carbonates is based primarily on data from warm‐water reef fishes, with efforts to assess global‐scale implications constrained by assumptions that this small cross section of the global fish community is widely representative. Here we test the extent to which temperature influences fish carbonate mineralogy and morphology by comparing products from temperate settings (20 species at temperatures spanning 10–18°C) against existing data (23–27°C). Overall, carbonate products were mineralogically, compositionally, and morphologically similar throughout the thermal range, and in most cases, we observed no differences within species (18 vs. 24°C) or families (10 vs. 25°C). Confirmation of within‐family consistency over large thermal gradients is significant because: (1) it facilitates a substantial range expansion over which fish carbonate production models can be constructed, even where family‐level product data are geographically limited; and (2) it implies that the solubility of products from any given fish family varies only due to local carbonate saturation states at excretion (and not crystallographic differences). The only exception was in two members of the Labridae (wrasses), which produced low‐Mg calcite (LMC) and minor amorphous calcium–magnesium carbonate (ACMC) at 10°C; the inverse of products from confamilials at 25°C. This finding could have significant implications for understanding the role of fish carbonates globally because ACMC is a highly unstable carbonate polymorph, whereas LMC is very stable.
Impacts of microplastic vs. natural abiotic particles on the clearance rate of a marine mussel
Authors: Harris Lyda S. T. , Carrington Emily 
Published: October 2019

AbstractIn coastal habitats, mussels are exposed to microplastics (MP; plastic 0.1 μm–5 mm) and silt, two abiotic particles that are similarly sized and lack nutrition. The addition of MP or silt may change the functional response of mussels. We measured clearance rate (CR) of Mytilus trossulus in three particle treatments (algae, MP + algae, and silt + algae) across four concentrations to (1) determine if the effects of MP and silt are similar and (2) disentangle the effects of particle type, particle concentration, and proportion of abiotic particles. CR decreased by 62% at high MP concentrations (> 1250 particles mL−1) but was not affected at equivalent silt concentrations. These findings suggest high MP concentrations inhibit mussel CR, more than expected by changes in particle concentration or the proportion of abiotic particles. As plastic production increases, mussel exposure to MP will increase, potentially reducing energy transfer, benthic‐pelagic coupling, and water clarity.
Radically Accessing D–A Type Ambipolar Copolymeric Materials with Intrinsic Electrical Conductivity and Visible–Near Infrared Absorption Via Electro‐Copolymerization
Authors: Ranathunge Tharindu A. , Karunathilaka Dilan , Ngo Duong T. , Attanayake Nuwan H. , Brodgon Phillip , Delcamp Jared H. , Rajapakse R. M. Gamini , Watkins Davita L. 
Published: October 2019

AbstractThienothiadiazole‐bisthiophene (TTDT2) and diketopyrrolo–pyrrole–bisthiophene (DPPT2) are successfully electro‐copolymerized with terthiophene (T3) as an initiator and linker at low oxidative potentials. AC impedance analysis, absorption spectroscopy, and elemental composition via SEM‐EDX support the formation of donor–acceptor (D–A) type alternating block copolymers, poly(T3‐TTDT2), and poly(T3‐DPPT2). Unique optical properties that span into the near infrared‐II(>1000 nm) region and inherent electrical conductivity at the p‐type regime, n‐type regime, and in between the two regimes (i.e., typical insulator region) are observed. This study showcases the advantages of electro‐polymerization toward tailoring of next generation opto‐electronic materials.
Evaluation of the occurrence of sporulating and nonsporulating pathogenic bacteria in manure and in digestate of five agricultural biogas plants
Authors: Le Maréchal Caroline , Druilhe Céline , Repérant Elisabeth , Boscher Evelyne , Rouxel Sandra , Le Roux Sophie , Poëzévara Typhaine , Ziebal Christine , Houdayer Catherine , Nagard Bérengère , Barbut Frédéric , Pourcher Anne‐Marie , Denis Martine 
Published: October 2019

AbstractThe number of agricultural biogas plants has been increasing in the past decades in some European countries. Digestates obtained after anaerobic digestion (AD) of manure are usually spread on agricultural land; however, their hygiene status regarding pathogens posing public health and/or animal health challenges has been poorly characterized up to now in France. In this study, three replicates of manure and digestate were collected from five farm biogas plants receiving animal manure in order to assess the occurrence and concentrations of sporulating (Clostridium botulinum, Clostridioides difficile, Clostridium perfringens) and nonsporulating (Listeria monocytogenes, thermotolerant Campylobacter spp., Salmonella, Escherichia coli, enterococci) bacteria. Concentrations of E. coli, enterococci, and C. perfringens in digestates ranged from 102 to 104, 104 to 105, and <103 to 7 × 105 CFU/g, respectively. Salmonella and C. difficile were detected in manure and digestate from the five biogas plants at concentrations ranging from <1.3 to >7 × 102 MPN/g and from 1.3 to 3 × 102 MPN/g, respectively. Thermotolerant Campylobacter, detected in all the manures, was only found in two digestates at a concentration of cells ranging from <10 to 2.6 × 102 CFU/g. Listeria monocytogenes and C. botulinum were detected in three manures and four digestates. The bacterial counts of L. monocytogenes and C. botulinum did not exceed 3 × 102 and 14 MPN/g, respectively. C. botulinum type B was detected at very low level in both the manure and digestate of farm biogas plants with no botulism history. The levels of pathogenic bacteria in both manure and digestate suggested that some bacteria can persist throughout AD.
Coordinated transient interaction of ZO‐1 and afadin is required for pedestal maturation induced by EspF from enteropathogenic Escherichia coli
Authors: Ugalde‐Silva Paul , Navarro‐Garcia Fernando 
Published: October 2019

AbstractEnteropathogenic Escherichia coli (EPEC) infection causes a histopathological lesion including recruitment of F‐actin beneath the attached bacteria and formation of actin‐rich pedestal‐like structures. Another important target of EPEC is the tight junction (TJ), and EspF induces displacement of TJ proteins and increased intestinal permeability. Previously, we determined that an EPEC strain lacking EspF did not cause TJ disruption; meanwhile, pedestals were located on the TJ and smaller than those induced by the wild‐type strain. Therefore, EspF could be playing an important role in both phenotypes. Here, using different cell models, we found that EspF was essential for pedestal maturation through ZO‐1 disassembly from TJ, leading to (a) ZO‐1 recruitment to the pedestal structure; no other main TJ proteins were required. Recruited ZO‐1 allowed the afadin recruitment. (b) Afadin recruitment caused an afadin–ZO‐1 transient interaction, like during TJ formation. (c) Afadin and ZO‐1 were segregated to the tip and the stem of pedestal, respectively, causing pedestal maturation. Initiation of these three discrete phases for pedestal maturation functionally and physically required EspF expression. Pedestal maturation process could help coordinate the epithelial actomyosin function by maintaining the actin‐rich column composing the pedestal structure and could be important in the dynamics of the pedestal movement on epithelial cells.
Mutational change of CTX‐M‐15 to CTX‐M‐127 resulting in mecillinam resistant Escherichia coli during pivmecillinam treatment of a patient
Authors: Nielsen Karen Leth , Hansen Katrine Hartung , Nielsen Jesper Boye , Knudsen Jenny Dahl , Schønning Kristian , Frimodt‐Møller Niels , Hertz Frederik Boëtius , Jansåker Filip 
Published: October 2019

AbstractPivmecillinam (amdinocillin pivoxil) is the recommended first‐choice antibiotic used to treat urinary tract infections (UTIs) in Denmark. The frequency of mutation to mecillinam (MEC) resistance is described as high in vitro; however, treatment of UTI has a good clinical response and prevalence of mecillinam resistance in Escherichia coli remains low despite many years of use. We describe occurrence of in vivo mecillinam resistance in a clinical isolate of ESBL‐producing E. coli following pivmecillinam treatment. The identified phenotypic differences in the mecillinam resistant isolate compared with the original mecillinam susceptible isolate were a full‐length LPS with O‐antigen (O25), mecillinam resistance and a lower MIC for ceftazidime. Regarding genotype, the resistant isolate differed with a mutation in blaCTX‐M‐15 to blaCTX‐M‐127, loss of a part of a plasmid and a genomic island, respectively, and insertion of a transposase in wbbL, causing the rough phenotype. The observed mecillinam resistance is expected to be caused by the mutation in blaCTX‐M‐15 with additional contribute from the serotype shift. We continue to recommend the use of pivmecillinam as first‐line treatment for UTI.
Prodromal Marker Progression in Idiopathic Rapid Eye Movement Sleep Behavior Disorder: Sample Size for Clinical Trials
Authors: Alotaibi Fatimah , Pelletier Amélie , Gagnon Jean‐François , Montplaisir Jacques Y. , Postuma Ronald B. 
Published: October 2019

AbstractObjectiveTo estimate prodromal marker progression in idiopathic rapid eye movement sleep behavior disorder patients with prodromal Parkinson's disease (PD) to calculate sample size for neuroprotective trials.MethodsPatients with polysomnogram‐proven idiopathic rapid eye movement sleep behavior disorder were assessed for prodromal PD using Movement Disorder Society criteria. We prospectively measured progression rates of numerous clinical variables, including motor, cognitive, special sensory, and autonomic variables and calculated the sample size required to demonstrate slowing of progression under 3 effectiveness assumptions (30%, 50%, and 70% slowing).ResultsOverall, the variables that progressed with lowest sample size requirements were motor variables (234 participants required per group for 50% efficacy over 2 years). By contrast, cognitive, special sensory, and autonomic variables showed modest progression with high variability, resulting in high sample sizes. The most efficient design was a time‐to‐event analysis using milestones of motor and cognitive decline (126 per group).ConclusionIn idiopathic rapid eye movement sleep behavior disorder, time‐to‐event analysis assessing milestones of decline is the most efficient trial design for neuroprotective therapy. © 2019 International Parkinson and Movement Disorder Society
Mutational spectrum of Mexican patients with tyrosinemia type 1: In silico modeling and predicted pathogenic effect of a novel missense FAH variant
Authors: Ibarra‐González Isabel , Fernández‐Lainez Cynthia , Alcántara‐Ortigoza Miguel Angel , González‐Del Angel Ariadna , Fernández‐Henández Liliana , Guillén‐López Sara , Belmont‐Martínez Leticia , López‐Mejía Lizbeth , Varela‐Fascinetto Gustavo , Vela‐Amieva Marcela 
Published: October 2019

AbstractBackgroundTyrosinemia type 1 (HT1, MIM#276700) is caused by a deficiency in fumarylacetoacetate hydrolase (FAH) and it is associated with severe liver and renal disfunction. At present, the mutational FAH (15q25.1, MIM*613871) spectrum underlying HT1 in the Mexican population is unknown. The objective of this study was to determine the FAH genotypes in eight nonrelated Mexican patients with HT1, who were diagnosed clinically.MethodsSequencing of FAH and their exon–intron boundaries and in silico protein modeling based on the crystallographic structure of mouse FAH.ResultsWe identified pathogenic variants in 15/16 studied alleles (93.8%). Nine different variants were found. The most commonly detected HT1‐causing allele was NM_000137.2(FAH):c.3G > A or p.(?) [rs766882348] (25%, n = 4/16). We also identified a novel missense variant NM_000137.2(FAH):c.36C > A or p.(Phe12Leu) in a homozygous patient with an early and fatal acute form. The latter was classified as a likely pathogenic variant and in silico protein modeling showed that Phe‐12 residue substitution for Leu, produces a repulsion in all possible Leu rotamers, which in turn would lead to a destabilization of the protein structure and possible loss‐of‐function.ConclusionHT1 patients had a heterogeneous mutational and clinical spectrum and no genotype–phenotype correlation could be established.
Breakpoint delineation in 5p‐ patients leads to new insights about microcephaly and the typical high‐pitched cry
Authors: Chehimi Samar N. , Zanardo Évelin A. , Ceroni José R. M. , Nascimento Amom M. , Madia Fabrícia A. R. , Dias Alexandre T. , Filho Gil M. N. , Montenegro Marília M. , Damasceno Jullian , Costa Thaís V. M. M. , Gasparini Yanca , Kim Chong A. , Kulikowski Leslie D. 
Published: October 2019

AbstractBackgroundCri du chat syndrome (CdCS) is a rare syndrome caused by a partial or complete deletion of the short arm of chromosome 5 (5p‐). The main clinical features include a high‐pitched cry, facial asymmetry, microcephaly, round face at birth, epicanthal folds, hypotonia, delayed growth and development.MethodsWe studied 14 Brazilian patients with CdCS using genomic array in order to better define the 5p breakpoints and recognize copy number variations (CNVs) that contribute to clinical manifestations associated with the syndrome.ResultsArray confirmed terminal deletions in 13 patients and an interstitial deletion in one patient. It was also possible to map the breakpoints and associate a genomic region of 4.7 Mb to the development of head circumference and cat‐like cry. We also found other CNVs concomitant to the 5p deletion including a 9p duplication, a 17q deletion, and a 22q deletion in three different patients.ConclusionWith advancements of molecular cytogenomic methods in the last two decades, it was possible to evidence cryptic alterations and improve the genotype–phenotype correlation. In this work, we describe a new genomic region associated with microcephaly and cat‐like cry and highlight the importance of precise delineation of 5p deletion breakpoints and detection of other CNVs in CdCS patients to improve genotype–phenotype correlation to perform a complete clinical and molecular diagnosis.
Weighted gene coexpression network analysis identifies a new biomarker of CENPF for prediction disease prognosis and progression in nonmuscle invasive bladder cancer
Authors: Shi Jiawei , Zhang Pu , Liu Lilong , Min Xiaobo , Xiao Yajun 
Published: October 2019

AbstractBackgroundThe dreadful prognosis of nonmuscle invasive bladder cancer mainly results from the delay in recognition of individuals with a high risk of progression. Thus, the emphasis of this work lies in developing valuable biomarkers that is conducive to accurately predicting the progression of NMIBC.MethodsMicroarray data from GSE32894 including 209 NMIBC samples were performed by weighted gene coexpression network analysis (WGCNA), which could find modules of highly correlated genes and relate modules to external sample traits. Besides, we constructed a protein–protein interaction to facilitate screening the hub gene. At last, we used RNA‐seq and microarray data and clinical information from ArrayExpress (E‐MTAB‐4321) and GSE13507 to select and validate the candidate gene.ResultsIn current paper, blue module of 13 gene coexpression clusters we identified was selected as the key modules. Seven genes namely: CDCA8, CENPF, MCM6, MELK, PRC1, STIL, and TPX2 have been identified as candidate genes. Notably, among them, only elevated CENPF in NIMBC tissue was closely associated with low progression‐free survival (PFS) and overall survival (OS) rate in three datasets and had a large area under receiver operating characteristic (ROC) curve. Finally, CENPF was identified as an effective biomarker in NMIBC.ConclusionTherefore, our findings submit a new progressive and prognostic molecular marker and therapeutic target for NMIBC. Moreover, these genes that deserve to be further researched may improve the comprehension about the occurrence and development of superficial bladder cancer.