bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of conserved and novel miRNAs responsive to heat stress in flowering Chinese cabbage using high-throughput sequencing
Authors: Ahmed Waqas , Xia Yanshi , Zhang Hua , Li Ronghua , Bai Guihua , Siddique Kadambot H. M. , Guo Peiguo 
Published: October 2019

AbstractPlant microRNAs (miRNAs) are noncoding and endogenous key regulators that play significant functions in regulating plant responses to stress, and plant growth and development. Heat stress is a critical abiotic stress that reduces the yield and quality of flowering Chinese cabbage (Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee). However, limited information is available on whether miRNAs are involved in the regulation of heat stress in B. campestris. A high-throughput sequencing approach was used to identify novel and conserved heat-responsive miRNAs in four small RNA libraries of flowering Chinese cabbage using leaves collected at 0 h, 1 h, 6 h and 12 h after a 38 °C heat-stress treatment. The analysis identified 41 conserved miRNAs (belonging to 19 MIR families), of which MIR156, MIR159, MIR168, MIR171 and MIR1885 had the most abundant molecules. Prediction and evaluation of novel miRNAs using the unannotated reads resulted in 18 candidate miRNAs. Differential expression analysis showed that most of the identified miRNAs were downregulated in heat-treated groups. To better understand functional importance, bioinformatic analysis predicted 432 unique putative target miRNAs involved in cells, cell parts, catalytic activity, cellular processes and abiotic stress responses. Furthermore, the Kyoto Encyclopedia of Genes and Genomes maps of flowering Chinese cabbage identified the significant role of miRNAs in stress adaptation and stress tolerance, and in several mitogen-activated protein kinases signaling pathways including cell death. This work presents a comprehensive study of the miRNAs for understanding the regulatory mechanisms and their participation in the heat stress of flowering Chinese cabbage.
Identification and Validation of a Novel Biologics Target in Triple Negative Breast Cancer
Authors: Wali Vikram B. , Patwardhan Gauri A. , Pelekanou Vasiliki , Karn Thomas , Cao Jian , Ocana Alberto , Yan Qin , Nelson Bryce , Hatzis Christos , Pusztai Lajos 
Published: October 2019

AbstractThe goal of this study was to identify a novel target for antibody-drug conjugate (ADC) development in triple negative breast cancer (TNBC), which has limited treatment options, using gene expression datasets andin vitrosiRNA/CRISPR andin vivofunctional assays. We analyzed 4467 breast cancers and identified GABRP as top expressed gene in TNBC with low expression in most normal tissues. GABRP protein was localized to cell membrane with broad range of receptors/cell (815–53,714) and expressed by nearly half of breast cancers tissues. GABRP gene knockdown inhibited TNBC cell growth and colony formationin vitroand growth of MDA-MB-468 xenografts in nude mice. Commercially available anti-GABRP antibody (5–100 μg/ml) orde novogenerated Fabs (20 μg/ml) inhibited TNBC cell growthin vitro. The same antibody conjugated to mertansine (DM1) also showed significant anticancer activity at nanomolar concentrations. Our results indicate that GABRP is a potential novel therapeutic target for ADC development.
Transcriptomic analysis reveals potential genes involved in tanshinone biosynthesis in Salvia miltiorrhiza
Authors: Chang Yujie , Wang Meizhen , Li Jiang , Lu Shanfa 
Published: October 2019

AbstractTanshinones are important bioactive components in Salvia miltiorrhiza and mainly accumulate in the periderms of mature roots. Tanshinone biosynthesis is a complicated process, and little is known about the third stage of the pathway. To investigate potential genes that are responsible for tanshinone biosynthesis, we conducted transcriptome profiling analysis of two S. miltiorrhiza cultivars. Differential expression analysis provided 2,149 differentially expressed genes (DEGs) for further analysis. GO and KEGG analysis showed that the DEGs were mainly associated with the biosynthesis of secondary metabolites. Weighted gene coexpression network analysis (WGCNA) was further performed to identify a “cyan” module associated with tanshinone biosynthesis. In this module, 25 cytochromes P450 (CYPs), three 2-oxoglutarate-dependent dioxygenases (2OGDs), one short-chain alcohol dehydrogenases (SDRs) and eight transcription factors were found to be likely involved in tanshinone biosynthesis. Among these CYPs, 14 CYPs have been reported previously, and 11 CYPs were identified in this study. Expression analysis showed that four newly identified CYPs were upregulated upon application of MeJA, suggesting their possible roles in tanshinone biosynthesis. Overall, this study not only identified candidate genes involved in tanshinone biosynthesis but also provided a basis for characterization of genes involved in important active ingredients of other traditional Chinese medicinal plants.
Inducing molecular isomerization assisted by water
Authors: Wang Dongsheng , Zhao Lei , Zhao Haiquan , Wu Jiazun , Wagner Manfred , Sun Wen , Liu Xiaodong , Miao Mao-sheng , Zheng Yonghao 
Published: October 2019

AbstractLight is not the only stimulus that can induce linear-to-cyclic isomerization of donor-acceptor Stenhouse adducts (DASAs). Here we demonstrate the water-induced linear-to-cyclic isomerization of DASAs. The mechanism of the water-induced linear-to-cyclic isomerization of DASAs is investigated by density functional theory (DFT) calculations. Water molecules coordinate with DASAs and stabilize the intermediates and cyclic isomers, which favors cyclization thermodynamically. Moreover, the linear-to-cyclic isomerization is reversible. Heating removes the coordinated H2O molecules, which further triggers cyclic-to-linear isomerization. DASAs have been applied in information hiding/displaying and color switching under water vapor and heating control.
Topological superconductivity with deformable magnetic skyrmions
Authors: Garnier Maxime , Mesaros Andrej , Simon Pascal 
Published: October 2019

AbstractMagnetic skyrmions are nanoscale spin configurations that are efficiently created and manipulated. They hold great promises for next-generation spintronics applications. In parallel, the interplay of magnetism, superconductivity and spin-orbit coupling has proved to be a versatile platform for engineering topological superconductivity predicted to host non-abelian excitations, Majorana zero modes. We show that topological superconductivity can be induced by proximitizing skyrmions and conventional superconductors, without need for additional ingredients. Apart from a previously reported Majorana zero mode in the core of the skyrmion, we find a more universal chiral band of Majorana modes on the edge of the skyrmion. We show that the chiral Majorana band is effectively flat in the physically relevant parameter regime, leading to interesting robustness and scaling properties. In particular, the number of Majorana modes in the (nearly-)flat band scales with the perimeter length of the system, while being robust to local disorder.
Perspektiven für junge Ärztinnen und Ärzte in der Infektiologie
Authors: Raspe M. , Rolling T. , Leisse C. , Fischer J. , Lehmann C. 
Published: October 2019

ZusammenfassungDie Infektiologie ist ein Querschnittsfach mit Verbindungen zu vielen medizinischen Disziplinen und bietet interessierten Ärztinnen und Ärzten eine Vielzahl von interessanten Betätigungsfeldern. Das Spektrum umfasst sowohl die ambulante und stationäre Patientenversorgung als auch grundlagenorientierte, klinische und epidemiologische Forschung. Der Bedarf an infektiologisch weitergebildeten Ärztinnen und Ärzten steigt, sodass die Berufsaussichten als sehr gut einzuschätzen sind. Die Vereinbarkeit von Beruf und Familie in der Infektiologie ist vergleichsweise günstig. Mit diesem Artikel wollen wir bei jungen Kolleginnen und Kollegen Interesse an diesem vielseitigen und spannenden Querschnittsbereich wecken und Berufsperspektiven aufzeigen. Daten aus einer Mitgliederbefragung der Deutschen Gesellschaft für Infektiologie e. V. geben Einblicke, in welchen Bereichen und mit welchen Tätigkeitsschwerpunkten Infektiologen heute überwiegend tätig sind.
Marketing – strategisch agieren statt resigniert
reagieren
Authors: Kernke Sylvia E. 
Published: October 2019

ZusammenfassungEin strategisches Praxismarketing ermöglicht es Arztpraxen, nicht einfach nur
mehr Patienten zu bekommen, sondern eine andere Patientenklientel zu
erschließen. Ein wichtiger Faktor für wirtschaftlichen Erfolg. Um im
Zukunftsmarkt Gesundheit herauszustechen, müssen Praxen ihre Individualität
herausstellen und diese auf allen Ebenen des Marketings ihren Patienten
kommunizieren.
Whole-exome sequencing of cervical carcinomas identifies activating ERBB2 and PIK3CA mutations as targets for combination therapy
Authors: Zammataro Luca , Lopez Salvatore , Bellone Stefania , Pettinella Francesca , Bonazzoli Elena , Perrone Emanuele , Zhao Siming , Menderes Gulden , Altwerger Gary , Han Chanhee , Zeybek Burak , Bianchi Anna , Manzano Aranzazu , Manara Paola , Cocco Emiliano , Buza Natalia , Hui Pei , Wong Serena , Ravaggi Antonella , Bignotti Eliana , Romani Chiara , Todeschini Paola , Zanotti Laura , Odicino Franco , Pecorelli Sergio , Donzelli Carla , Ardighieri Laura , Angioli Roberto , Raspagliesi Francesco , Scambia Giovanni , Choi Jungmin , Dong Weilai , Bilguvar Kaya , Alexandrov Ludmil B. , Silasi Dan-Arin , Huang Gloria S. , Ratner Elena , Azodi Masoud , Schwartz Peter E. , Pirazzoli Valentina , Stiegler Amy L. , Boggon Titus J. , Lifton Richard P. , Schlessinger Joseph , Santin Alessandro D. 
Published: October 2019

Significance
The development of novel, effective treatments for patients with advanced/recurrent cervical cancer remains an unmet medical need. Using whole-exome sequencing, we identified multiple genes with recurrent alterations in cervical cancer, including the ERBB2/PI3K/AKT/mTOR, apoptosis, chromatin remodeling, and cell cycle pathways. Using fully sequenced cell lines and xenografts, we found pan-HER (afatinib/neratinib) and PIK3CA (copanlisib) inhibitors to be active, but only transiently effective in controlling the in vivo growth of PIK3CA-mutated cervical tumor xenografts. In contrast, the combination of irreversible pan-HER kinase and PIK3CA inhibitors was highly synergistic and able to induce durable regression of xenografts harboring derangements in the ERBB2/PI3K/AKT/mTOR pathway in vivo. These findings suggest a large subset of cervical tumors (>70%) might benefit from existing ERBB2/PIK3CA/AKT/mTOR-targeted drugs.
Leptomycin B inhibits the proliferation, migration, and invasion of cultured gastric carcinoma cells
Authors: Zhu Hepan , Yang Yi , Wang Li , Xu Xiaobin , Wang Tingting , Qian Haoran 
Published: October 2019

ABSTRACT
Chromosome region maintenance 1 (CRM1) plays a critical role in tumorigenesis and progression through modulating nuclear export of several proteins. However, the precise effects of CRM1 inhibitor on gastric carcinoma have not yet been illustrated. Here, we investigated the potential anti-cancer activities of leptomycin B, the most potent CRM1 antagonist, on cultured gastric carcinoma cells. Our findings demonstrate that CRM1 was highly expressed in four gastric carcinoma cell lines. Leptomycin B inhibited the viability of HGC-27 and AGS cells in a dose- and time-dependent pattern. Leptomycin B at the dose of 10 nM or 100 nM suppressed the migration and invasion of HGC-27 and AGS cells. Leptomycin B elevated the expressions of autophagy-related protein LC3-II and autophagy substrate p62. Moreover, leptomycin B enhanced the LC3-positive puncta formation in cells. Our data suggest that leptomycin B may exert an anti-cancer activity possibly through interfering autophagy function in gastric carcinoma cells.
“Safer-by-design” synthesis of quantum dots in flow reactors
Authors: Akdas Tugce , Reiss Peter 
Published: October 2019

Abstract
After decades of research and commercialization efforts, colloidal semiconductor nanocrystals (quantum dots, QDs) are now on the verge of widespread application. Given the high demands of industrial production processes regarding process safety, cost efficiency and reproducibility of high quality products with high yield, continuous processes in flow reactors offer several advantages over the scale-up of a batch reaction. In this contribution, we discuss safety and operational issues for QD synthesis in micro- and millifluidic reactors using two case studies, i.e. synthesis of the two cadmium-free material systems, namely InP and CuInS2. While current “Safe-by-Design” (SbD) discussions concerning nanomaterials mainly focus on material types and their toxicity, thus on the functionality of the final products, we will assess the production process as such, considering all chemicals involved in the various stages. In our contribution we discuss synthesis and downstream processing of core QDs and thereby show that flow synthesis of QDs can result in a “greener” and safer production routine.
Toxicity removal by Daphinia similis assay in BTEX contaminated groundwater using nanometric TiO2/ZrO2 film and black light
Authors: Donaire Patricia P.R. , Assalin Marcia R. , Durán Nelson 
Published: October 2019

Abstract
In this work, a photocatalytic tubular reactor, with a nanometric TiO2/ZrO2 film, immobilized on glass substrate, was developed in order to investigate the degradation of volatile organic compounds, such as, benzene, toluene, ethylbenzene and xylene (BTEX ) in groundwater sample, under black light irradiation (365nm). The BTEX compound were determined by solid phase microextraction (SPME) technique and analyzed by Gas Chromatography - Ionization Flame Detection (CG-FID). The efficiency of photocatalytic process was evaluated by determination of BTEX removal and toxicity, using Daphnia similis as organism-test. The influence of the initial concentration of BTEX in the degradation process was investigated. The degradation process of sample groundwater contaminated with BTEX (145μg/L) from gasoline, resulted in 90% of efficiency after 120 minutes of treatment. The toxicity (EC50-48 h) of the groundwater sample to Daphnia similis was 14.1% (10.5-19.0) for untreated samples. After 30 min of treatment, the EC50-48 h was of 84.3 (71-100%). The benzene efficiency removal was reduced in function of the increase of its initial concentrations that observation was not found for the other compounds studied.
Synthesis, characterization and cytotoxicity of chitosan-coated Fe3O4 nanoparticles functionalized with ascorbic acid for biomedical applications
Authors: Lourenço I M , Pelegrino M T , Pieretti J C , Andrade G P , Cerchiaro G , Seabra A B 
Published: October 2019

Abstract
Iron oxide magnetic nanoparticles (Fe3O4 NPs) are used to drive and to promote sustained release of drugs in target sites. Biocompatibility and superparamagnetic behaviour are important features to the successful biomedical applications of Fe3O4 NPs. In this study, Fe3O4 NPs were synthesized by the co-precipitation method and coated with chitosan (CS) containing ascorbic acid (AA), allowing formation of Fe3O4@CS-AA NPs. The antioxidant AA was used as a drug model. The synthesized NPs were characterized by different techniques. The results showed the formation of spherical nanoparticles with average diameter of 67.22 ± 0.82 nm, at solid state, as analysed by atomic force microscopy (AFM). The NPs were found to have a superparamagnetic behaviour at room temperature, and the presence of CS-AA on the surface of Fe3O4 NPs did not affect the superparamagnetic behaviour of the nanoparticles. The in vitro AA release assay showed a sustained release of the model drug from Fe3O4@CS-AA NPs for at least 48 h. In addition, cytotoxicity assays for Fe3O4 NPs and Fe3O4@CS-AA NPs did not show significant toxicity towards mammary epithelium (MCF-10A) cell line after 24 h of incubation. This present study demonstrated the successful synthesis of superparamagnetic and biocompatible Fe3O4@CS-AA NPs, which are able to release the model drug in a sustained manner. Thus, this nanomaterial might act as a nanocarrier in target drug release.
OncoTherad: A New Nanobiological Response Modifier, its Toxicological and Anticancer Activities
Authors: Durán Nelson , Dias Queila C. , Fávaro Wagner J. 
Published: October 2019

Abstract
This study reports the effects of a promising therapeutic option for non-muscle invasive bladder cancer (NMIBC) based on OncoTherad intravesical immunotherapy in an appropriated animal model. OncoTherad is a nanostructured inorganic phosphate complex associated to glycosidic protein, which exhibits immunomodulatory and antitumor properties. Biochemical parameters in rats, mice and rabbits treated intravesically with OncoTherad at doses of 20-100 mg/kg, did not differed statistically from their respective controls, exhibiting no systemic toxic effects. All the target organs did not present inflammation and histopathological changes. NMIBC was induced by treating female Fischer 344 rats with N-methyl-N-nitrosourea (MNU). Bacillus Calmette-Guérin (BCG) were used as positive control in the animal models. The results demonstrated that animals treated with OncoTherad distinctly showed a significant histopathological recovery from the cancer state of animals (80%) when compared to BCG treatment. In addition, BCG and OncoTherad intravesical immunotherapies were able to restore TLR2 levels. However, OncoTherad increased of TLR4 levels when compared to BCG. Thus, the activation of TLR4 by Oncotherad was efficient in reducing urothelial neoplastic progression. All data are indicative that of OncoTherad is a feasible candidate for the NMIBC treatment.
Nanotoxicity and Dermal Application of Nanostructured Lipid Carrier Loaded with Hesperidin from Orange Residue
Authors: Durán Nelson , Costa Amanda F. , Stanisic Danijela , Bernardes Juliana S. , Tasic Ljubica 
Published: October 2019

Abstract
Flavonoids are a class of polyphenolic compounds that can be extracted from citrus fruits. Because of their features, they have been considered as new bullets in cancer therapy. Flavonoids present anti-oxidant, anti-inflammatory and anti-cancer properties and some studies are pointing that their higher intake involves lower risks of cancer. The nanostructured lipid carriers (NLC) are drug delivery systems composed from a mixture of solid and liquid lipids that increase physical stability and promote the sustained release of the drug. So, the main aim of this research was to combine the potential of the flavonoid as a natural anti-oxidant compound with the nanostructured lipid carriers to form an efficient system for flavonoid delivery into the cells. These NLC were prepared through high pressure homogenization technique and were evaluated in function of several standard properties. The samples exhibited good stability through time and temperature, with sizes of around 215 nm and zeta potentials of -35 mV during a period of 105 days. The encapsulation and the loading efficiency were respectively 96% and 2.25% up to 105 days of storage. In vitro release assays showed a burst process for NLC-L pointing to their shell structure with flavonoid situated in the contour of the particles. The cell viability tests showed that flavonoid, either free or loaded into NLC, did not present in vitro toxicity to the melanoma cells tested: A375, CHL01 and SKMEL147 through 3-(4,5-dimethyiltiazol-2yl)-2,5-diphenyl tetrazolium bromide (MTT), neutral red and crystal violet assays. Hesperidin at 45 μmol L−1 was able to reduce significantly the radical forming up to 92.58 ± 0.82% in DPPH assay. On the other hand, the produced NLC were applied in a skin lotion formulation, which showed a good stability and opened up a new application for the produced nanoparticles as in anti-aging and moisturizing cosmetics. Finally, the in vitro cytotoxicity tests were carried out using several human culture cells. The flavonoid loaded and empty nanocarriers have been used to treat the cells. All data suggested that, at the level studied, non-significant toxicities on cells were observed.
Methods for Assessing the Thermodynamic Characteristics of the Combustion Process Using the Indicator Diagram in Spark-Ignition Engines
Authors: Smolenskii V V , Smolenskaya N M 
Published: October 2019

Abstract
The paper deals with the evaluation of the thermodynamic characteristics of the combustion process according to the experimentally obtained indicator diagram of pressure in spark ignition engines. The proposed method allows to determine the main characteristics of the combustion process (change in internal energy, heat generation characteristic, speed and intensity of combustion processes in the flame front, volume of the burned mixture, volume of unburned mixture) using the experimental indicator diagram of pressure obtained for a spark ignition engine.
Classification of Cyber and Physical Systems of Industry 4.0
Authors: Zakoldaev D A , Gurjanov A V , Shukalov A V , Zharinov I O 
Published: October 2019

Abstract
The actual task is to create the classification system for cyber and physical technological equipment for smart factories of the Industry 4.0. Smart factories are a new type of production companies which work automatically. To project a smart factory they need to choose samples of cyber and physical systems of different purpose to unite them in automatic sections. To solve the choice task of production machine samples they need to create libraries of technical and tactical characteristics and its ontology description the base of which is the system to classify cyber and physical equipment. There is a classification scheme of industrial cyber and physical systems to work in a smart factory of item designing. The classification systems base is types of technological operations being realized in a company and also methods and technologies which the cyber and physical systems use. Cyber and physical systems functionality is described for mechanical treatment and assembly workshops of item designing company.
Technologies for Creating Geographic Information Resources for Monitoring the Socio-Ecological Conditions of Cities
Authors: Yeprintsev S A , Shekoyan S V , Lepeshkina L A , Voronin A A , Klevtsova M A 
Published: October 2019

Abstract
Geo-information resources have been developed for monitoring socio-ecological conditions (using the example of cities in Central Russia). The technologies of the developed GIS allow to take into account the variability of scale, the transformation of cartographic projections, changing the composition of the mapped objects, the formation of queries in numerous databases in real time, variations in the ways of displaying objects. The developed GIS system consists of hierarchically subordinate database sections and thematic cartographic tools reflecting the natural resource potential, the socio-economic and environmental-hygienic situation in the cities of Central Russia.
Modeling a Stress Condition of Ore Massif in the Process of Bauxite Deposits Development in the Mines of «Sevuralboksitruda»
Authors: Kosukhin N I , Sidorov D V , Gil L B 
Published: October 2019

Abstract
The publication examines the problems of concentrator of elevated tension formation in pillars and ore massif. The article presents the results of modeling the stress state of interchamber pillars and ore deposits, using specialized geomechanical software PRESS 3D URAL. In the process of numerical experiments, it was found that the increase of natural flexibility in pillars leads to a decrease of normal stresses in comparison with the variant of a stiff scheme. If interchamber pillar involves different varieties of ores, there is a redistribution of stress on the site with more stiff ores. The article shows that the stress state control of interchamber pillars and ore massif in conditions of OJSC «Sevuralboxitruda» can be achieved by drilling relief holes.