bluuebunny/crossref_metadata_2025_split huggingface dataset:
Probability Distribution of Turbulent Kinetic Energy Dissipation Rate in Stratified Turbulence: Microstructure Measurements in the Southern California Bight
Authors: Lozovatsky Iossif , Shearman Kipp , Pirro Annunziata , Fernando H. J. S. 
Published: June 2019

AbstractA month of microstructure measurements in the Southern California Bight allowed to confirm the hypothesis that in ocean pycnocline, below the surface mixed layer, the probability distribution of turbulent kinetic energy dissipation rate ε averaged over equal time segments (2 s with 512‐Hz sampling rate, corresponding to about 1.4‐m vertical averaging) follows the Burr distribution suggested by Lozovatsky, Fernando, et al. (2017, https://doi.org/10.1002/2017JC013076). The bin‐median estimates of ε was well correlated with the medians of the Burr model. The parameters of the Burr distribution varied in time, but no relationship was found between these variations and the changes in wind patterns and phases of barotropic tide. In the range 10−9~ < ε < 10−8 W/kg, the dissipation rate could be related to internal wave activity in the region.
A 3,2-Hydroxypyridinone-based Decorporation Agent that Removes Uranium from Bones In Vivo
Authors: Wang Xiaomei , Dai Xing , Shi Cen , Wan Jianmei , Silver Mark A. , Zhang Linjuan , Chen Lanhua , Yi Xuan , Chen Bizheng , Zhang Duo , Yang Kai , Diwu Juan , Wang Jianqiang , Xu Yujie , Zhou Ruhong , Chai Zhifang , Wang Shuao 
Published: June 2019

AbstractSearching for actinide decorporation agents with advantages of high decorporation efficiency, minimal biological toxicity, and high oral efficiency is crucial for nuclear safety and the sustainable development of nuclear energy. Removing actinides deposited in bones after intake is one of the most significant challenges remaining in this field because of the instantaneous formation of highly stable actinide phosphate complexes upon contact with hydroxyapatite. Here we report a hydroxypyridinone-based ligand (5LIO-1-Cm-3,2-HOPO) exhibiting stronger affinity for U(VI) compared with the reported tetradentate hydroxypyridinone ligands. This is further revealed by the first principles calculation analysis on bonding between the ligand and uranium. Both in vitro uranium removal assay and in vivo decorporation experiments with mice show that 5LIO-1-Cm-3,2-HOPO can remove uranium from kidneys and bones with high efficiencies, while the decorporation efficiency is nearly independent of the treatment time. Moreover, this ligand shows a high oral decorporation efficiency, making it attractive for practical applications.
Coevolution of vocal signal characteristics and hearing sensitivity in forest mammals
Authors: Charlton Benjamin D. , Owen Megan A. , Swaisgood Ronald R. 
Published: June 2019

AbstractAlthough signal characteristics and sensory systems are predicted to co-evolve according to environmental constraints, this hypothesis has not been tested for acoustic signalling across a wide range of species, or any mammal sensory modality. Here we use phylogenetic comparative techniques to show that mammal vocal characteristics and hearing sensitivity have co-evolved to utilise higher frequencies in forest environments – opposite to the general prediction that lower frequencies should be favoured in acoustically cluttered habitats. We also reveal an evolutionary trade-off between high frequency hearing sensitivity and the production of calls with high frequency acoustic energy that suggests forest mammals further optimise vocal communication according to their high frequency hearing sensitivity. Our results provide clear evidence of adaptive signal and sensory system coevolution. They also emphasize how constraints imposed by the signalling environment can jointly shape vocal signal structure and auditory systems, potentially driving acoustic diversity and reproductive isolation.
The ionospheric irregularities climatology over Svalbard from solar cycle 23
Authors: De Franceschi Giorgiana , Spogli Luca , Alfonsi Lucilla , Romano Vincenzo , Cesaroni Claudio , Hunstad Ingrid 
Published: June 2019

AbstractThe paper presents an unprecedented description of the climatology of ionospheric irregularities over the Arctic derived from the longest Global Navigation Satellite Systems data series ever collected for this specific aim. Two TEC and scintillation receivers are working at Ny-Ålesund (Svalbard, NO), the first of which has been installed in late September 2003. They sample the L1 and L2 signals at 50 Hz from all the GPS satellites in view. The receivers monitor an area of about 600 km radius that includes the auroral and cusp/cap regions in the European longitudinal sector. The length of the data series and the privileged site of observation allow describing the Arctic ionosphere along about two solar cycles, from the descending phase of cycle 23 to almost the end of cycle 24. Our analysis results into a detailed assessment of the long-term behaviour of the ionosphere under solar maximum and solar minimum conditions, including several periods of perturbed ionospheric weather caused by unfavourable helio-geophysical conditions. Since November 2015, a multi-constellation GNSS receiver has been deployed in Ny-Ålesund, providing the opportunity to perform the ionospheric climatology from Galileo signals. The results offer realistic features of the high latitude ionosphere that can substantially contribute to the necessary improvements of forecasting models, providing a broad spectrum of ionospheric reactions to different space weather conditions.
The impact of levodopa therapy-induced complications on quality of life in Parkinson’s disease patients in Singapore
Authors: Wu Jonathan , Lim Ee-Chien , Nadkarni Nivedita V. , Tan Eng-King , Kumar Prakash M. 
Published: June 2019

AbstractThe objective of this study was to investigate the impact of levodopa therapy-induced complications on the quality of life (QoL) of Parkinson’s disease (PD) patients in Singapore over a 1-year follow-up period. 274 PD patients were prospectively recruited, of which 78 patients completed the follow-up. Patients were evaluated on: (1) motor symptoms, (2) non-motor symptoms, (3) levodopa therapy-induced complications and (4) QoL. Levodopa-induced complications including dyskinesia and OFF symptoms occurred in 13.5% and 55.9% of the study population, respectively. In patients who completed the 1-year follow-up, there was a trend suggestive of increasing dyskinesia duration, more disabling dyskinesia as well as longer, more sudden and unpredictable OFF periods. There was a significant decline in the overall QoL at follow-up, in particular, activities of daily living, emotional well-being, cognition and communication domains were the most affected. The multivariable analysis demonstrated that worsening of UPDRS IV total score over 1-year interval was associated with worsening in PDQ-Summary Index score (d = 0.671, p = 0.014). In conclusion, levodopa-induced complications had significant adverse impacts on QoL. This study substantiates the importance for clinicians to closely monitor and promptly manage levodopa therapy-induced complications that may arise in patients.
Live imaging of Aiptasia larvae, a model system for coral and anemone bleaching, using a simple microfluidic device
Authors: Van Treuren Will , Brower Kara K. , Labanieh Louai , Hunt Daniel , Lensch Sarah , Cruz Bianca , Cartwright Heather N. , Tran Cawa , Fordyce Polly M. 
Published: June 2019

AbstractCoral reefs, and their associated diverse ecosystems, are of enormous ecological importance. In recent years, coral health has been severely impacted by environmental stressors brought on by human activity and climate change, threatening the extinction of several major reef ecosystems. Reef damage is mediated by a process called ‘coral bleaching’ where corals, sea anemones, and other cnidarians lose their photosynthetic algal symbionts (family Symbiodiniaceae) upon stress induction, resulting in drastically decreased host energy harvest and, ultimately, coral death. The mechanism by which this critical cnidarian-algal symbiosis is lost remains poorly understood. The larvae of the sea anemone, Exaiptasia pallida (commonly referred to as ‘Aiptasia’) are an attractive model organism to study this process, but they are large (∼100 mm in length, ∼75 mm in diameter), deformable, and highly motile, complicating long-term imaging and limiting study of this critical endosymbiotic relationship in live organisms. Here, we report ‘Traptasia’, a simple microfluidic device with multiple traps designed to isolate and image individual, live larvae of Aiptasia and their algal symbionts over extended time courses. Using a trap design parameterized via fluid flow simulations and polymer bead loading tests, we trapped Aiptasia larvae containing algal symbionts and demonstrated stable imaging for >10 hours. We visualized algae within Aiptasia larvae and observed algal expulsion under an environmental stressor. To our knowledge, this device is the first to enable time-lapsed, high-throughput live imaging of cnidarian larvae and their algal symbionts and, in further implementation, could provide important insights into the cellular mechanisms of cnidarian bleaching under different environmental stressors. The ‘Traptasia’ device is simple to use, requires minimal external equipment and no specialized training to operate, and can easily be adapted using the trap optimization data presented here to study a variety of large, motile organisms.
Characterization of Porcine Hepatic and Intestinal Drug Metabolizing CYP450: Comparison with Human Orthologues from A Quantitative, Activity and Selectivity Perspective
Authors: Schelstraete Wim , Clerck Laura De , Govaert Elisabeth , Millecam Joske , Devreese Mathias , Deforce Dieter , Bocxlaer Jan Van , Croubels Siska 
Published: June 2019

AbstractOver the past two decades, the pig has gained attention as a potential model for human drug metabolism. Cytochrome P450 enzymes (CYP450), a superfamily of biotransformation enzymes, are pivotal in drug metabolism. Porcine CYP450 has been demonstrated to convert typical substrates of human CYP450. Nevertheless, knowledge and insight into porcine CYP450 quantity and substrate selectivity is scant, especially regarding intestinal CYP450. The current study aimed to map the quantities of hepatic and intestinal CYP450 in the conventional pig by using a proteomic approach. Moreover, the selectivity of the six most common used probe substrates (phenacetin, coumarin, midazolam, tolbutamide, dextromethorphan, and chlorzoxazone) for drug metabolizing enzyme subfamilies (CYP1A, CYP2A, CYP3A, CYP2C, CYP2D and CYP2E respectively), was investigated. Hepatic relative quantities were 4% (CYP1A), 31% (CYP2A), 14% (CYP3A), 10% (CYP2C), 28% (CYP2D) and 13% (CYP2E), whereas for the intestine only duodenal CYP450 could be determined with 88% for CYP3A and 12% for CYP2C. Furthermore, the results indicate that coumarin (CYP2A), midazolam (CYP3A), tolbutamide (CYP2C), and dextromethorphan (CYP2D) are as selective for porcine as for human CYP450. However, phenacetin (CYP1A2) and chlorzoxazone (CYP2E1) are less selective for the specific enzyme, despite similarities in selectivity towards the different enzymes involved compared to humans.
Effect of annealing on a pseudogap state in untwinned YBa2Cu3O7−δ single crystals
Authors: Solovjov A. L. , Petrenko E. V. , Omelchenko L. V. , Vovk R. V. , Goulatis I. L. , Chroneos A. 
Published: June 2019

AbstractThe effect of annealing both in the oxygen atmosphere and at room temperatures on physical properties such as the pseudogap (Δ*(T)) and excess conductivity (σ′(T)) of untwined YBa2Cu3O7−δ (YBCO) single crystal with a small deviation from oxygen stoichiometry is studied. It was revealed that as the charge carrier density, nf, increases, Тс also slightly increases, whereas the temperature of the pseudogap opening, T*, decreases noticeably, which is consistent with the phase diagram (PD) of cuprates. The excess conductivity in the vicinity of Tc is represented by the Aslamazov-Larkin and Hikami-Larkin fluctuation theories, illustrating the three-dimensional to two-dimensional (i.e. 3D-2D) crossover with an increase in temperature. The crossover temperature T0 determines the coherence length along the c axis is ξc(0) = 0.86 Å, that is 2.6 times larger than for optimally doped YBCO single crystals with defects. Taking into account the short coherence length in high-temperature superconductors, in the model of free charge carriers the phase relaxation time of fluctuating Cooper pairs is determined, τφ (100 K) = (4.55 ± 0.4) · 10−13 s, which is slightly (1.2 times) larger than in well-structured YBCO films, and as in films, does not depend on nf. It is shown that Δ*(T) at different annealing stages practically does not change its shape. As in the well-structured YBCO films, Δ*(T) demonstrates maximum at Tpair~124 K which depends weakly on nf. However, the maximum value of Δ*(Tpair) increases with increasing nf, as it follows from the PD of cuprates. Comparing the experimental data with the Peters-Bauer theory we estimated the density of local pairs <n↑n↓> ≈ 0.3 near Tc that is a common value for high-temperature superconductors.
Fuzzy petrology in the origin of carbonatitic/pseudocarbonatitic Ca-rich ultrabasic magma at Polino (central Italy)
Authors: Lustrino Michele , Luciani Natascia , Stagno Vincenzo 
Published: June 2019

AbstractThe small upper Pleistocene diatreme of Polino (central Italy) is known in literature as one of the few monticellite alvikites (volcanic Ca-carbonatite) worldwide. This outcrop belongs to the Umbria-Latium Ultra-alkaline District (ULUD), an area characterized by scattered and small-volume strongly SiO2-undersaturated ultrabasic igneous rocks located in the axial sector of the Apennine Mts. in central Italy. Petrographic and mineralogical evidences indicate that Polino olivine and phlogopite are liquidus phases rather than mantle xenocrysts as instead reported in literature. The presence of monticellite as rim of olivine phenocrysts and as groundmass phase indicates its late appearance in magma chambers at shallow depths, as demonstrated by experimental studies too. The absence of plagioclase and clinopyroxene along with the extremely MgO-rich composition of olivine (Fo92–94) and phlogopite (average Mg# ~93) suggest for Polino magmas an origin from a carbonated H2O-bearing mantle source at depths at least of 90–100 km, in the magnesite stability field. In contrast with what reported in literature, the ultimate strongly ultrabasic Ca-rich whole-rock composition (~15–25 wt% SiO2, ~31–40 wt% CaO) and the abundant modal groundmass calcite are not pristine features of Polino magma. We propose that the observed mineral assemblage and whole-rock compositions result mostly from the assimilation of limestones by an ultrabasic melt at a depth of ~5 km. A reaction involving liquidus olivine + limestone producing monticellite + CO2 vapour + calcite is at the base of the origin of the Polino pseudocarbonatitic igneous rocks.
Cockade structures as a paleo-earthquake proxy in upper crustal hydrothermal systems
Authors: Berger Alfons , Herwegh Marco 
Published: June 2019

AbstractCockades are clasts completely surrounded by spheroidal hydrothermal overgrowth rims. They are observed inside hydrothermal fault breccias and can provide insights into fault dynamics. The formation of cockades with spheroidal hydrothermal overgrowth rims is related to fast fracturing and dilation, and requires primary clasts to be suspended in a fluid. The rim growth is driven by drops in fluid pressure and related oversaturation. We use descriptions of cockades, their rims and cements in a fault breccia. Geometrical data are combined with mechano-chemical calculations to gain insights into seismic processes and estimate seismic magnitudes. Fast rates for formation of cockade cores and first rim growth are interpreted to be the result of an earthquake’s main shock. Younger growth rims represent subsequent aftershocks, while cemented cockades record interseismic periods. We propose that by considering growth rates of hydrothermal precipitates and cements, paleo-earthquake cycles can be unraveled and a link between geophysics and fault structures can be established.
Accommodation, slip inversion, and fault segmentation in a province-scale shear zone from high-resolution, densely spaced wide-aperture seismic profiling, Centennial Valley, MT, USA
Authors: Bruno Pier Paolo G. , Berti Claudio , Pazzaglia Frank J. 
Published: June 2019

AbstractWe acquired a ~9-km long, high-resolution reflection seismic profile in the Centennial Valley, Montana, to better understand the kinematics of basin bounding faults and their role in accommodating proposed right-lateral shear in the Northern Basin and Range adjacent to the Yellowstone hotspot. In pursuing these goals, our findings have also shed light on the development of hanging wall stratigraphy and seismic hazards for this part of the SW Montana seismic belt. Here we present the profile and a working interpretation that identifies fault inversion, and an oblique, anticlinal accommodation zone linking the Centennial and Lima Reservoir faults in the Centennial Valley. These interpretations are consistent with seismicity and GPS-geodetically observed right-lateral shear aligned with the Centennial Valley north of the Yellowstone hotspot. Data were acquired using dense, wide-aperture arrays and illuminate the subsurface stratigraphy and faults down to ~1200 m, showing that the basin is a half-graben with a southern depocenter driven by the listric geometry of the north-dipping Centennial fault. Reflectors onlap basement highs with growth geometry against these faults. Our interpretation of a bright basal reflection as the Timber Hill Basalt (~6 Ma) or related flow, is consistent with a late Miocene – Pliocene inception of the basin proposed by other research. We also note a small inversion structure that we interpret as local evidence of transpression in the shear zone. This transpression is part of the accommodation zone and seismogenic faults including the Lima Reservoir fault that has well-expressed Holocene surface ruptures a few kilometres west of the seismic line along the northern edge of the Centennial basin.
EGF receptor (EGFR) inhibition promotes a slow-twitch oxidative, over a fast-twitch, muscle phenotype
Authors: Ciano Margherita , Mantellato Giada , Connolly Martin , Paul-Clark Mark , Willis-Owen Saffron , Moffatt Miriam F. , Cookson William O. C. M. , Mitchell Jane A. , Polkey Michael I. , Hughes Simon M. , Kemp Paul R. , Natanek S. Amanda 
Published: June 2019

AbstractA low quadriceps slow-twitch (ST), oxidative (relative to fast-twitch) fiber proportion is prevalent in chronic diseases such Chronic Obstructive Pulmonary Disease (COPD) and is associated with exercise limitation and poor outcomes. Benefits of an increased ST fiber proportion are demonstrated in genetically modified animals. Pathway analysis of published data of differentially expressed genes in mouse ST and FT fibers, mining of our microarray data and a qPCR analysis of quadriceps specimens from COPD patients and controls were performed. ST markers were quantified in C2C12 myotubes with EGF-neutralizing antibody, EGFR inhibitor or an EGFR-silencing RNA added. A zebrafish egfra mutant was generated by genome editing and ST fibers counted. EGF signaling was (negatively) associated with the ST muscle phenotype in mice and humans, and muscle EGF transcript levels were raised in COPD. In C2C12 myotubes, EGFR inhibition/silencing increased ST, including mitochondrial, markers. In zebrafish, egfra depletion increased ST fibers and mitochondrial content. EGF is negatively associated with ST muscle phenotype in mice, healthy humans and COPD patients. EGFR blockade promotes the ST phenotype in myotubes and zebrafish embryos. EGF signaling suppresses the ST phenotype, therefore EGFR inhibitors may be potential treatments for COPD-related muscle ST fiber loss.
Ultrasound-mediated blood-brain barrier opening enhances delivery of therapeutically relevant formats of a tau-specific antibody
Authors: Janowicz Phillip W. , Leinenga Gerhard , Götz Jürgen , Nisbet Rebecca M. 
Published: June 2019

AbstractThe microtubule-associated protein tau is an attractive therapeutic target for the treatment of Alzheimer’s disease and related tauopathies as its aggregation strongly correlates with disease progression and is considered a key mediator of neuronal toxicity. Delivery of most therapeutics to the brain is, however, inefficient, due to their limited ability to cross the blood-brain barrier (BBB). Therapeutic ultrasound is an emerging non-invasive technology which transiently opens the BBB in a focused manner to allow peripherally delivered molecules to effectively enter the brain. In order to open a large area of the BBB, we developed a scanning ultrasound (SUS) approach by which ultrasound is applied in a sequential pattern across the whole brain. We have previously shown that delivery of an anti-tau antibody in a single-chain variable fragment (scFv) format to the brain is increased with SUS allowing for an enhanced therapeutic effect. Here we compared the delivery of an anti-tau antibody, RN2N, in an scFv, fragment antigen-binding (Fab) and full-sized immunoglobulin G (IgG) format, with and without sonication, into the brain of pR5 tau transgenic mice, a model of tauopathy. Our results revealed that the full-sized IgG reaches a higher concentration in the brain compared with the smaller formats by bypassing renal excretion. No differences in either the ultrasound-mediated uptake or distribution in the brain from the sonication site was observed across the different antibody formats, suggesting that ultrasound can be used to successfully increase the delivery of therapeutic molecules of various sizes into the brain for the treatment of neurological diseases.
Early alterations in a mouse model of Rett syndrome: the GABA developmental shift is abolished at birth
Authors: Lozovaya N. , Nardou R. , Tyzio R. , Chiesa M. , Pons-Bennaceur A. , Eftekhari S. , Bui T.-T. , Billon-Grand M. , Rasero J. , Bonifazi P. , Guimond D. , Gaiarsa J.-L. , Ferrari D. C. , Ben-Ari Y. 
Published: June 2019

AbstractGenetic mutations of the Methyl-CpG-binding protein-2 (MECP2) gene underlie Rett syndrome (RTT). Developmental processes are often considered to be irrelevant in RTT pathogenesis but neuronal activity at birth has not been recorded. We report that the GABA developmental shift at birth is abolished in CA3 pyramidal neurons of Mecp2−/ymice and the glutamatergic/GABAergic postsynaptic currents (PSCs) ratio is increased. Two weeks later, GABA exerts strong excitatory actions, the glutamatergic/GABAergic PSCs ratio is enhanced, hyper-synchronized activity is present and metabotropic long-term depression (LTD) is impacted. One day before delivery, maternal administration of the NKCC1 chloride importer antagonist bumetanide restored these parameters but not respiratory or weight deficits, nor the onset of mortality. Results suggest that birth is a critical period in RTT with important alterations that can be attenuated by bumetanide raising the possibility of early treatment of the disorder.