bluuebunny/crossref_metadata_2025_split huggingface dataset:
Therapeutic effect of human umbilical cord-derived multipotent mesenchymal stromal cells in a patient with Crigler–Najjar syndrome type I
Authors: Sukhikh G. T. , Degtyareva A. V. , Silachev D. N. , Gorunov K. V. , Dubrovina I. V. , Ushakova L. V. , Degtyarev D. N. 
Published: September 2019

The article presents the results of intravenous transplantation of allogeneic multipotent mesenchymal stromal cells, derived from a human umbilical cord, to a child with Crigler–Najjarsyndrome type I during the first 2 years of life. The therapy is aimed at reduction of the duration of phototherapy while maintaining a safe level of serum bilirubin.In this study, a five-day-old child with the bilirubin level of 340 µmol/l was treated with phototherapy for 16–18 hours daily in the neonatal period. Then, phototherapy was reduced to 14–16 hours. The level of bilirubin varied from 329 to 407 μmol/l. At the age of 2 months, it was decided to use multipotent mesenchymal stromal cells with a significant decrease in the duration of phototherapy up to 2 hours a day. During the observation period (2 years at the time of writing this article) the child received 6 injections of multipotent mesenchymal stromal cells. A positive effect developed within 4–7 days after administration and persisted for 2–3 months. There were no side effects or complications during and after transplantation.Thus, intravenous transplantation of multipotent mesenchymal stromal cells is an effective treatment of Crigler–Najjar syndrome type I; it reducesthe need for phototherapy,significantly improvesthe quality of life of the patients and prolongstheir life with native liver.
Features of the state of newborns born to underage mothers
Authors: Mikhailin E. S. , Ivanova L. A. 
Published: September 2019

The purpose of this work was to determine the features of the state of newborns of underage mothers. The children of minor pregnant women were examined (n=483), who were observed and gave birth to the Maternity Hospital No. 10 in St. Petersburg in 2004–2014. [1 group – 13–15 years old (n=49); 2 group – 16–17 years old (n=434)]. The comparison group consisted of 110 children of women of medium reproductive age. Significant differences in the anthropometric characteristics of newborns between groups were not found. It was shown that in children of minors of both age groups the hypotrophy of the newborn was significantly more frequently observed, as well as inflammatory diseases specific to the perinatal period (neonatal infection of the skin, conjunctivitis). Minors 13–15 years old were significantly more likely than 16–17 year olds and women of medium reproductive age to have asphyxia during labor and cephalhematoma.
Hypoxic syndrome in hereditary diseases of connective tissue
Authors: Yurieva E. A. , Vozdvizhenskaya E. S. , Kushnareva M. V. , Semyachkina A. N. , Kharabadze M. N. , Sukhorukov V. S. 
Published: September 2019

Objective. To analyze the influence of clinical and biochemical markers on the diagnostic algorithm for hypoxic syndrome in children with genetic diseases of connective tissue.Characteristics of children and methods. There were examined 60 children were examined: 50 children with Ehlers–Danlossyndrome and 10 children with Marfan syndrome.Results. Constant fatigue, fast fatigue, low tolerance to physical exercises, pale skin, muscle hypotonia and headache are the most frequent symptoms of a hypoxic state in children with connective tissue dysfunction. At the biochemical level, hypoxic syndrome was characterized by signs of oxidative stress. The authors noted an decrease in pO2 , an increase in pCO2 in the circulating blood, an increase in the activity of anaerobic glycolysis with accumulation of lactate and pyruvate, a decrease in the synthesis of macroergs (ATP, ADP, AMP) and the activity of the mitochondrial respiratory enzymes, the emersion of mitochondrial insufficiency in the form of ripped red fibers (RRF) in myocytes , conglomerates of lipids, calcium, glycogen. There was an increased content of peroxidation products in the blood, urine and saliva.Conclusion. Periodic use of antihypoxants, drugs with detoxifying and immunomodulatory effect is justified for the treatment of children.
Analysis of the perinatal risk causes and the structure of pathology in premature children in Chelyabinsk, born with extremely low and very low body weight
Authors: Uzunova A. N. , Onishcenko N. A. 
Published: September 2019

The purpose of the study is to analyze the history and structure of the pathology of premature infants with extremely low body weight and very low body weight under dynamic follow up in the monitoring department for children of the perinatal risk group. Using the method of continuous sampling we analyzed observation maps of 95 premature infants with extremely low body weight and very low body weight of gestation age from 24 to 36 weeks.All newborns have a low Apgar score at birth and at the 5th minute, and Apgar score was lower in children with extremely low body weight as compared with children with very low body weight. All children had congenital anomalies of the organs and systems, marked signs of immaturity, with prevailing changes in the cardiovascular system, passability of the renal pelvis, and congenital deformity of thigh.Among the conditions that occurred in the perinatal period, the authors diagnosed intraventricular hemorrhage in 50.5% of patients, 89.6% of them had hemorrhage of grade II – III, all children had respiratory distress syndrome. Bronchopulmonary dysplasia was diagnosed in 60% of premature infants, its moderate and severe forms in 1/3 of the patients. All children had anemia, and 13.7% of children had a neonatal syndrome from mothers with diabetes mellitus. 1/3 of premature infants had retinopathy; its frequency did not depend on the body weight at birth. All children had a combination of pathologies. Bronchopulmonary dysplasia was combined with respiratory distress syndrome and anemia in 100% of children. The combination of bronchopulmonary dysplasia with intraventricular hemorrhage was more often recorded in patients with extremely low body weight than in patients with very low body weight.
Statistical analysis on the removal of malachite green dye using active carbons of Achyranthes aspera and Allamanda blanchetii plants
Authors: Ravulapalli Sujitha , Kunta Ravindhranath 
Published: September 2019

Abstract
Activated carbons were prepared from the stem parts of Achyranthes aspera and Allamanda blanchetii plants and were investigated as adsorbents for the removal of malachite green dye from contaminated water. Various extraction conditions such as pH, initial concentration of dye, adsorbent dosage, temperature, agitation time and presence of co-ions were optimized for the maximum possible extraction of the dye. For analyzing the combined effect of these parameters on the removal efficiency of the adsorbents, statistical optimization modelling was adopted. The adsorbents developed were characterized and the adsorption abilities were observed to be 40.0 mg/g and 53.0 mg/g for the active carbons of Achyranthes aspera and Allamanda blanchetii plants respectively. The mechanism of adsorption was studied using various isotherm models and it was found that the Freundlich model describes well the adsorption process. Thermodynamic studies revealed the endothermic and spontaneous nature of physisorption. The kinetics of adsorption were well defined by the pseudo-second-order model. Desorption and regeneration studies of the spent adsorbents indicated that the percentage of extraction has not come down below 80.0% even after five regenerations for both the adsorbents. The validity of the methods developed are tested with real dye-polluted industrial effluent samples.
Research on the water level in a bending channel using a guide wall
Authors: Zhang Jing , Zhang Qinghua , Wang Tiantian , Yang Jinmeng , Li Xuemei 
Published: September 2019

AbstractIn order to study the water level at the convex and concave banks after installing a guide wall in a spillway chute bend, with the original condition that the Fr at the entrance of the channel bend is larger than 1.0 (supercritical flow) when there is no the guide wall, systematic experiments with the guide wall were conducted for three radii (2.4B, 3.2B and 4B; B is the width of the channel), bottom slopes (0.01, 0.005 and 0.02), and discharges (50, 100 and150 m3 h−1). Results show that, firstly, after installing a guide wall, the Fr becomes smaller and even lower than 1.0, which means the flow status changes from supercritical to subcritical in some conditions with the help of the guide wall. Secondly, the water depth at the convex bank decreases with the increase of the relative axial radius while this presents to be adverse at the concave bank. Thirdly, for water surface differences in cross-sections, the maximum value decreases with the increase of the relative axial radius, and increases with the increase of the discharge per unit width or the bottom slope. Additionally, a novel formula for calculating the maximum water surface difference was obtained in this article.
Formulation and In-Vitro Evaluation of Buclizine Buccoadhesive Tablets
Authors: Wathore Sandeep Ashokrao 
Published: September 2019

Buclizine is an antihistamine that is used to treat motions sickness, especially to prevent and treat symptoms such as abdominal pain, dizziness, nausea, vomiting, headaches, etc. It may also be used either alone or in a combination with other medicines for the treatment of hay fever and other allergic conditions. These dosages form make close contact with buccal mucosa, avoid first- pass metabolism, increase absorption and patient compliance, results in an enhanced drug bioavailability. Buccal adhesive tablets were prepared by direct compression. Carbopol 940 and Sodium Alginate was used in different ratios and also in combination. Formulated 10 batches were evaluated for physical parameter, swelling studies, bioadhesion studies, and in-vitro drug release and in-vitro drug permeation. Bioadhesion studies were carried out to determine mucoadhesive potential of prepared tablets. Tablets were evaluated for in-vitro drug release for 6 hrs, using USP type II method and. From these results it can be concluded that formulation batch F6 was most promising comprising of 1:4 ratio of Sodium Alginate. The best fit model for the optimized batch F6 was zero order having R value 0.9889 and K value is 0.2542.
Keywords: Buclizine, Carbopol 940, Sodium Alginate, buccal tablet, in-vitro drug permeation
Analytical method development and validation of Amlodipine besylate in tablet dosage form
Authors: Kumar Sunil , Ram Bigan 
Published: September 2019

The accurate and precise HPLC analytical method validated for the determination of Amlodipine besylate in pharmaceutical dosage form. The chromatographic separation is carried out on shimadzu HPLC system (LC-2010 CHT) with UV Vissible detector and C18(150mm x3.9 mm) 5 μm Column. The Mobile phase consists of Acetonitrile: Methanol: PH 3.0 Buffer (15 V: 35 V: 50 V) , at the flow rate of 1.0 ml/min and elutes were monitoring at 237 nm. The observed retention time for Amlodipine besylate was 12.3 min. The % RSD for system precision was 0.41 % and Method precision was 0.58 %. The method was found to linear (R=0.99996) in the Concentration range of 35-105 μg/ml (50 to 150%). The accuracy was in between 99.50-99.91%.
Keywords: HPLC, Correlation coefficient, System suitability, Bias, % RSD and ICH guidelines
ANALYTICAL METHOD EVELOPMENT AND VALIDATION FOR ANTI ASTHAMATIC DRUG OXYMETAZOLINE HYDROCHLORIDE IN NASAL SPRAY FORMULATIONS BY RP-HPLC
Authors: Kulkarni Pankaj N , Dodake-Supekar Alaknanda M , Nipte Amol S , Jadhav Chetan K , Gill Charansingh H. 
Published: September 2019

A new, simple, accurate and economic reverse-phase HPLC method has been developed for quantification of Oxymetazoline Hydrochloride in nasal spray formulations. This developed method has been validated according to International Conference on Harmonization (ICH) guideline with respect to system suitability, specificity, precision, linearity, accuracy, and robustness. An isocratic condition of mobile phase Phosphate buffer (pH 3.0): Acetonitrile in a ratio of 60:40, v/v at a flow rate of 1.0 mL/minute over RP C18 (octadecylsilane (ODS), 250 × 4.6 mm, 5 µm, ECLIPSE X DB C-18) column at ambient temperature was maintained. This method is specific and showed excellent linear response with correlation coefficient (R2) values of 0.999, which was within the limit of correlation coefficient (R2 0.995). A simple and accurate reversed-phase HPLC method for the analysis of Oxymetazoline Hydrochloride in nasal spray formulations was developed and validated successfully.
Extracellular Protease and DNase Activities in Clinical and Environmental Isolates of Cryptococcus neoformans Species Complex from Central India
Authors: Gumasta Richa , Nawange Shesh Rao , Singh Shankar Mohan , Garg Abhijeet , Sethi Ruchi , Yadu Ruchika 
Published: September 2019

Enzymes are important not only for the growth and multiplication of the microorganism but also in the infection, penetration of the host tissue and encountering host defense mechanisms. This study aims to investigate extracellular protease and DNase activity in clinical (20) and 120 environmental isolates of C. neoformans species complex collected from different localities of central India. DNase test agar containing toluidine blue and Yeast Carbon Base (YCB) agar medium supplemented with 0.1% BSA + 0.01% polypeptone was employed for the screening of DNase and protease production respectively. DNase and protease production was detected by the appearance of clear zones around the colonies. On the basis of enzymatic activity and their Pz values, high protease production (Pz≤0.6) was observed by 14 (11.6 %) environmental and 4 (11.6 %) clinical strains on 5th day, whereas 35 (29.16 %) environmental and 8 (40 %) clinical strains were screened on 8th day of incubation. Similarly 13 (10.83%) environmental and 3 (15 %) clinical strains on the 5th day, however 32 (26.66%) environmental and 8 (40 %) clinical strains on the 8th day of incubation were found to be high DNase producing strains with low Pz value (Pz≤0.6). In the case of protease activity, no significant difference was observed whereas a significant difference has shown by clinical C. neoformans and C. gattii strains on the 5th day of DNase production (p < .001). Extracellular enzymes play a vital role in the pathogenicity and virulence of C. neoformans species complex, therefore, enzymes are considered as worthy targets for developing therapeutics.
Keywords: Cryptococcus neoformans species complex, Extracellular enzymes, DNase, protease, virulence, Pz value
Formulation and In-Vitro Evaluation of Taste Masked Fast Disintegrating Tablets of Labetalol Hydrochloride by Wet Granulation Technique
Authors: Shahidulla SM , Jeelani Tayyaba 
Published: September 2019

Labetalol Hydrochloride is a β-blocker generally indicated for the treatment of hypertension, and it is extensively metabolized due to the hepatic metabolism. In the present work, an attempt was made to mask the taste by Solid Dispersion technique, with a formulation into Fast Disintegrating dosage form, using superdisintegrants such as Cross carmellose sodium (CCS), crospovidone (CP) and sodium starch glycolate (SSG). The complexes of Labetalol hydrochloride with HP-β-CD (1:3 ratio) were prepared by Co-precipitation method. Using the drug HP-β-CD complex, Fast Disintegrating tablets were prepared by Wet granulation Technique and evaluated for hardness, friability, weight variation, thickness, disintegrating time (DT), In-vitro dispersion time and dissolution rate. The results of Direct compression optimized formulation WG9 (Sodium Starch Glycolate 15mg and Starch paste 18mg) has shown the % Drug release of 99.97%, In-vitro Dispersion time of 16 Secs respectively.
Keywords: Solid dispersions, fast disintegrating tablets, Labetalol, Crospovidone, Croscarmellose sodium and Sodium starch glycolate.
Effects of Nicotine on Sperm Health and Semen Quality: A Review based on Animal Studies
Authors: Ray Dibyendu 
Published: September 2019

Various studies have revealed the toxic effects of nicotine, an alkaloid compound of tobacco, on semen quality and sperm physiology; however, these studies collectively are still unsummarized. Here, I systematically discuss and summarize the nicotine exposure, pharmacology and its insults on semen quality and sperm physiology. I searched the Web of Science, PubMed, and Scopus databases for only English language articles or abstracts from September 1969 to March 2019 (inclusive) using the key words “Nicotine” and “tobacco” versus “sperm”. In conclusion, the mainstream published work demonstrates the negative effects of nicotine on semen quality: first, by increasing sperm count, and by enhancing sperm motility and reducing sperm DNA damage, though there are a few in clinical studies that have deliberated some adverse effects. The detrimental effects of Nicotine on semen quality may be due to decreased functionality of reproductive organs, increased levels of oxidative damage to sperm, reduced amount of energy produced by spermatozoa. However, additional research, mainly clinical, is still needed to confirm these
Keywords: Nicotine; sperm; testicular damage; semen quality
Herbal Alternative for Kidney Stone Diseases
Authors: Patel Bhavisha Bhupendrabhai 
Published: September 2019

Kidney stone disease is an increasing disorder of humans. It affects about 12% of the world population. Epidemiological data have shown that calcium oxalate is the predominant mineral in a majority of kidney stones. [1] It has been associated with an increased risk of end-stage renal failure. Kidney stones result from a succession of several physicochemical events including super saturation, nucleation, growth, aggregation, and retention within the kidneys. Kidney stones may cause extreme pain and blockage of urine flow .The average life time risk of stone formation has been reported in the range of 5-10 %.Recurrent stone formation is a common part of the medical care of patients with stone disease.[2] Kidney stone disease is usually treated with medications that may cause a number of side-effects. Even improved and besides the high cost that imposes, compelling data now suggest that exposure to shock waves in therapeutic doses may cause acute renal injury, decrease in renal function and an increase in stone recurrence. Data from in vitro, in vivo and clinical trials reveal that phytotherapeutic agents could be useful as either an alternative therapy in the management of urolithiasis. The present review therefore critically explains the potential usefulness of herbal medicines in the management of urolithiasis.
Keywords: Kidney stones, Calcium oxalate, Herbal plant extracts, Alternative medicine
Formulation of Effective Microbial Consortium and Its Application for Industrial Wastewater Treatment
Authors: Mahilarasi A , Jaianand Kannaiyan , Rameshkumar K , Balaji Paulraj , Veeramanikandan Veeramani 
Published: September 2019

The present study was conducted for auto mobile industry, food industry and pharmaceutical industries waste water treatment using effective microbial consortium. The effective microorganisms like Acinetobacter pittii, Escherichia coli, Fictibacillus nanhaiensis, Lysinibacillus xylanilyticus and Planococcus maritimus were isolated from respective sources. The microbial consortium was formulated using molasses as medium at pH 3.8 and incubated at 37°C for 3 days. The results showed that the formulated consortium was efficient for industrial waste water treatment and thereby it reduced the environmental impact.
Keywords: Bio-remediation, Microbial consortium, Industrial waste water, Heavy metals
Case Report on Steroid Induced Cushing Syndrome
Authors: Salma Shaik 
Published: September 2019

Glucocorticoids are the effective steroid drugs which reduce the inflammation & most commonly glucocorticoids are used in diseases such as asthma, allergic diseases such as psoriasis, eczema and rheumatoid arthritis. Long term effect of corticosteroids leads to the symptoms of moon face, buffalo hump, pink stretch marks, and weight gain. In this we are going to report a case of 30 yrs female patient with representing the long term use of corticosteroids; Prednisolone leads to cushing syndrome. Eventually she was treated with oral hypoglycemics and diuretics and the use of steroid had stopped temporarily and the dose of prednisolone was tapered.
Keywords: glucocorticoids, prednisolone, Cushing syndrome
A Research Article Phytochemical and Pharmacological Evaluation of Carthamus tinctorius Leaves
Authors: Pant Rahul , Irchhaiya Raghuveer , Rao Ch. V. 
Published: September 2019

Hepatoprotective activity of methanolic extract of leaves of Carthmus tinctorius L. (MECT) was investigated against hepatotoxicity produced by administering a combination of two anti-tubercular drugs Isoniazid and Rifampicin for 24 days by Oral route in rats. MECT were administered at two graded dose i.e. 200 and 300 mg/kg p.o. 45 min after to anti-tubercular challenge for 24 days. MECT was evident in the all doses as there was a significant decrease in AST, ALT, ALP, and Total Bilirubin levels. MECT significantly decreased the level of these biochemical parameter as compared to control. HPTLC analysis showed the presence of B-sitosterol and Lupeol in MECT. Results showed that Carthamus tinctorius possess significant Hepatoprotective activity which might be due to flavonoids.
Keywords: Carthamus tinctorius leaves1, hepatoprotective2, Rifampicin3, Isoniazide4, SGPT5.
Anti-Trypanosomal Activity of Guiera senegalensis on Trypanosoma brucei Infected Mice
Authors: André Zongo , Hervé Vitouley Sèna , Zakaria Bengaly , Adama Kaboré , Aristide Traoré , Hamadou Tamboura Hamidou , Gaston Belem Adrien Marie 
Published: September 2019

Aqueous decoction of Guiera senegalensis leaves was studied orally and intraperitoneally for its antitrypanosomal activity on mice infected experimentally with Trypanosoma brucei brucei. After a phytochemical screening followed by an acute toxicity study on mice, the extract of plant was administered once daily for 2 days at doses of 60, 120 and 240 mg / kg orally and 15, 30 and 60 mg / kg intraperitoneally after infection. Then, parameters of parasitaemia, packed cell volume (PCV), mean survival time and body weight of the mice treated with the extract were measured and compared with positive (diminazene aceturate) and negative (distilled water) controls for 7 days in a row. Results indicate that the aqueous extract of G. senegalensis leaves contains tannins, flavonoids, saponosides, reducing compounds and anthocyanosides, alkaloids and coumarins. LD50 of the extract are 1264.49 mg / kg by oral route and 316.22 mg / kg by intraperitoneal route. The doses of 240 mg / kg by oral route and 15 and 60 mg / kg by intraperitoneal route of aqueous extract showed a mean survival time (5 days) comparable to the positive control. Parasitaemia level increased in all mice tested except in mice treated with diminazene aceturate during the post-infestation period. During this period, PCV and body weight of all mice decreased by both routes of administration. These results of the study show the pharmacological utility of G. senegalensis leaves in the control of TAA by herders / pastoralists and suggest continuing further bio-guided studies to isolate the active components of the plant in order to improve their efficiency.
Keywords: In vivo test; Trypanosoma brucei brucei; Guiera senegalensis leaves; phytochemical screening; acute toxicity.