bluuebunny/crossref_metadata_2025_split huggingface dataset:
Collagen-disruptive cell therapy: adoptive transfer of membrane-anchored, tumor cell surface vimentin-targeted interleukin 12–armed TILs suppress collagen expression to boost deep T-cell infiltration via dual signaling activation and significant CCKAR reduction.
Authors: Li Shulin 
Published: October 2024

Abstract

Tumor-targeted T-cell therapies of various types have been booming, but T-cell therapy is limited by its inability to penetrate the collagen barrier surrounding tumors. The destruction of tumor collagen is significant because collagen both suppresses T cells and contributes to the formation of the extracellular matrix. Our previously reported cell surface vimentin (CSV)–targeted and membrane-anchored IL12-armed (attIL12) T cells can reduce collagen production by killing cancer-associated fibroblasts, thereby increasing T-cell infiltration. However, attIL12-T cells cannot reduce collagen expression by tumors that highly express CCKAR. In this study, we discovered that CCKAR directly boosts collagen production by tumor cells in vitro and in vivo. attIL12-modified tumor-infiltrating lymphocytes (TILs) disabled collagen production by CCKAR-high autologous tumor cells in vitro and sarcoma patient-derived xenografts (PDXs) in vivo. This disruption of collagen production by tumor cells required a simultaneous interaction between the CSV on autologous tumor cells, which is targeted by attIL12, and HLA-TCR on attIL12-TILs; when either interaction was abrogated, collagen production and CCKAR expression were not shut down. Mechanistically, the interaction between attIL12-TILs and autologous tumor cells synergized IFNγ production, which in combination with CCKAR downregulation reduced collagen expression through suppression of both TGFβ-stimulated SMAD activation and CCKAR-AKT signaling. Diminishing collagen expression from tumor cells significantly increased T-cell infiltration and improved tumor growth inhibition in PDX sarcomas. This study thus uncovers the first tumor collagen–disrupting T-cell therapy we know of. This is significant because collagen is enriched in most high-grade CCKAR+ human sarcomas. Thus, this attIL12-TIL therapy holds great clinical potential for boosting T-cell infiltration in high-grade, collagen-rich tumors.
Rapidly recurring superior cerebellar artery aneurysm following embolization after neoadjuvant therapy for breast cancer: a case report
Authors: Pan Qichen , Gu Shujuan , Chen Hongyan , Zhao Xudong , Zhao Jiahui , Zou Cunyi 
Published: October 2024

Abstract

Background
Neoadjuvant therapy is becoming increasingly common for tumor treatment, yet its impact on aneurysm is rarely reported. We report an attractive case in which a superior cerebellar artery aneurysm rapidly recurred following embolization after neoadjuvant therapy for breast cancer.
Case presentation:
A 49-year-old female patient underwent superior cerebellar artery embolization and extraventricular drainage for subarachnoid and intraventricular hemorrhage. She had received two cycles of neoadjuvant therapy for breast cancer 40 days prior. Postoperatively, she was successfully discharged and received neoadjuvant therapy for one cycle again. Afterwards, she was readmitted due to hydrocephalus. DSA revealed the embolized aneurysm recurred, and secondary embolization and ventriculoperitoneal shunt surgery were performed. Four months later, this aneurysm had progressed and subsequently recurred. Considering the urgent need for treatment of breast cancer, we suggested that patient prioritized tumor treatment.
Conclusion
Clinicians should be alert that neoadjuvant therapy may promote the progression of cerebral aneurysms, especially those complicated by hydrocephalus.
Exact parallelized dynamic mode decomposition with Hankel matrix for large-scale flow data
Authors: Asada Hiroyuki , Kawai Soshi 
Published: October 2024

Abstract

An exact parallel algorithm of dynamic mode decomposition (DMD) with Hankel matrices for large-scale flow data is proposed.The proposed algorithm enables the DMD and the Hankel DMD for large-scale data obtained by high-fidelity flow simulations, such as large-eddy simulations or direct numerical simulations using more than a billion grid points, on parallel computations without any approximations.The proposed algorithm completes the computations of the DMD by utilizing block matrices of \(X^TX\inRR{k}{k}\) (where \(X\inRR{n}{k}\) is a large data matrix obtained by high-fidelity simulations, the number of snapshot data is \(n\gtrsim10^9\) , and the number of snapshots is \(k\lesssim O(10^3)\) ) without any approximations: for example, the singular value decomposition of \(X\) is replaced by the eigenvalue decomposition of \(X^TX\) .Then, the computation of $X^TX$ is parallelized by utilizing the domain decomposition often used in flow simulations, which reduces the memory consumption for each parallel process and wall-clock time in the DMD by a factor approximately equal to the number of parallel processes.The parallel computation with communication is performed only for $X^TX$, allowing for high parallel efficiency under massively parallel computations.Furthermore, the proposed exact parallel algorithm is extended to the Hankel DMD without any additional parallel computations, realizing the Hankel DMD of large-scale data collected by over a billion grid points with comparable cost and memory to the DMD without Hankel matrices.Moreover, this study shows that the Hankel DMD, which has been employed to enrich information and augment rank, is advantageous for large-scale high-dimensional data collected by high-fidelity simulations in data reconstruction and predictions of future states (while prior studies have reported such advantages for low-dimensional data).Several numerical experiments using large-scale data, including laminar and turbulent flows around a cylinder and transonic buffeting flow around a full aircraft configuration, demonstrate that (i) the proposed exact parallel algorithm reproduces the existing non-parallelized Hankel DMD, (ii) the Hankel DMD for large-scale data consisting of over a billion grid points is feasible by using the proposed exact parallel algorithm with high parallel efficiency on more than 6 thousand CPU cores, and (iii) the Hankel DMD has advantages for high-dimensional data such as $n\gtrsim10^9$.
Challenges Facing Proper Application of Interprofessional Education in Sudan: Perspectives of Candidates of Master of Health Professions Education (2020-2023)
Authors: Ahmed EMAN , ALI Qurashi 
Published: October 2024

Abstract

Introduction
Traditional medical and health schools used to teach students in separated setup. However, gap appeared between the evolving challenges of health requirements and the static educational system. The latter has failed to keep in pace with the everyday changes in health needs. Interprofessional education (IPE) indicates interaction between various professions and creates a strong base for teamwork and collaboration. The model of IPE is innovative method that might need planning and groundwork prior to execution.
Aim of the study
To determine the challenges that might face application of interprofessional education in Sudanese medical and health schools.
Research method
Design of the current study is descriptive cross-sectional study that conducted via questionnaire. The questionnaire divided into two parts. The first for personal information and second part to assess thoughts and views of the participants to the anticipated challenges for application of IPE in Sudan.
Population were candidates of program of health professions education in Sudan. One hundred and one candidates from various specialties participated in the study; (response rate of 51.53%) The results of statistical analysis use Statistical Package for the Social Sciences (SPSS) - version 28.
Results
Majority of participants believed that integrating 𝐈𝐏𝐄 curriculum content into existing curriculum represents an obstacle and suggested that learning approach and styles need adjustment for smooth implementation. Majority of participants supported that additional time is crucial to accommodate IPE curriculum. Significant differences in these finding differ according to academic rank, specialty involved and years of experience.
The participants believed in the negative impact of compromised funding, inadequate premises. Majority of the participants irrespective of their specialties, experience or rank assumed that enthused and motivated students -reasonable number of- would facilitate the application.
Conclusion
IPE is an innovative appealing model in adult education that needs meticulous planning and preparation prior to execution.
Clinical trial number: not applicable.
Total Antioxidant Capacity Status In Non-Obese Adolescent Females With PCOS: A cross section study
Authors: Zaki Moushira , Salem Sondos , Salama Ehab , Girgiss Mina Wassef , Elnahas Tamer , Sherity Safenaz Y. El , Youness Eman Refaat 
Published: October 2024

Abstract

Background
The endocrine disease polycystic ovarian syndrome (PCOS) has a number of complications. Teenagers all over the globe are becoming increasingly concerned about the syndrome. It has been suggested that oxidative stress plays a significant role in the pathogenesis of PCOS.
Aim
The purpose of the present work was to estimate Total antioxidant capacity (TAC) in non –obese adolescents and decide if the TAC could be a potential marker for the prediction and diagnosis of PCOS.
Methods
Serum samples were collected to assess the levels of follicle stimulant hormone (FSH), luteinizing hormone (LH), serum lipids, serum total antioxidant capacity (TAC) in 50 non-obese patients with PCOS and 50 controls; correlation analysis was made between serum lipid, hormonal parameters and TAC.
Results
Adolescents with PCOS had significantly lower levels of TAC compared with controls and showed significant higher values of LH, FSH and LH/FSH ratio. There were significant negative correlations between TAC levels with LH, FSH and LH/FSH. Linear regression analysis revealed statistically significant correlations between TAC and LH and FSH levels in non-obese adolescents with PCOS.
Conclusion
Serum TAC level was diminished in non-obese PCOS patients and was related to increase of LH/FSH ratio. So, quantification of TAC activity in serum could be of value in assessment of cases at higher risk for development of PCOS, suggesting its possible role in regulating sex hormones and could have potential roles in the etiopathogenesis of PCOS in non-obese adolescent females.
Analysis of the shear strength of a hydroxyapatite (HA)-coated commercially pure titanium (cp-Ti) implant: A in vivo study in pigs
Authors: noree Nikom , Tangpornprasert Pairat , virulsri Chanyaphan , Wangdee Chalika , Akaraphutiporn Ekkapol , Wilairat Vajara 
Published: October 2024

Abstract

We assess the shear strength of a commercially pure titanium (cp-Ti) implant coated with hydroxyapatite (HA) in the intramedullary canal of miniature pigs' femur. The research involved the utilization of cylinders coated with cpTi-HA, which had an average diameter of 9 mm and a length of 10 mm. The interfacial strength between the bone and the implant reached an ultimate value of 11.32 MPa. The procedure of implantation was performed on three miniature pigs, and the process of osteointegration was assessed using three-dimensional images and histomorphology. In order to examine any detachment, scanning electron microscopy (SEM) was utilized to inspect the complete interface between the implant and the bone. Our discoveries revealed a failure mode that resembled the removal of the femoral stem in a hip revision procedure at the bone-to-bone interface.
Advancing Phototherapies in Glioblastomas: Plasma Membrane Damage by Single-Point Laser Irradiation Using Clinical Cancer Markers.
Authors: Carrizo Cristina , Córdoba Jaime Fernández , Oña Ana , D´Agostino Gianluca , Thompson Sebastian 
Published: October 2024

Abstract

Purpose: This study aimed to present a novel phototherapeutic approach by inducing plasma membrane damage and cell death in glioblastoma cells using single-point laser irradiation rather than larger area irradiation.
Methods:
Glioblastoma cells were cultured in 2D monolayers and 3D spheroid models. Fluorescent markers like 5-aminolevulinic acid (5-ALA), the precursor of Protoporphyrin IX (PPIX), and Sodium Fluorescein (NaF) aid in tumor visualization during surgery are being explored for photodynamic therapy (PDT). 5-ALA and NaF were applied to induce PPIX production or dye uptake, respectively. Fluorescence microscopy confirmed dye localization—PPIX on the plasma membrane and NaF in the extracellular space. Cells were irradiated with a focused laser, and the resulting plasma membrane damage was assessed through viability assays and membrane integrity markers. Cell death was quantified in both monolayers and spheroids.
Results:
PPIX was localized on the plasma membrane, while NaF remained in the extracellular space, contacting the membrane. Single-point laser irradiation induced plasma membrane disruption in both conditions, resulting in selective cancer cell death. Both dyes led to significant cell death, even at the center of spheroids, demonstrating the effectiveness of this method in 3D structures.
Conclusions:
This study presents a novel approach using focused laser irradiation and clinically approved dyes to induce precise, targeted cell death in glioblastoma models, suggesting a promising potential for theranostic applications in tumor eradication.
Kimura disease: comprehensive analysis of clinical features, comorbidities, and treatment approaches
Authors: Zhu Wenjiao , Zhang Lei , Zhang Jing , Wang Wei , Xiang Ye , He Dingxian , Shi Jing , Liang Yiran , Shi Yuanping , Ning Xianhui , Ye Ling , Jin Meiling 
Published: October 2024

Abstract

Background
Kimura disease (KD) is a rare, chronic, inflammatory disorder characterized by painless subcutaneous masses, lymphadenopathy, elevated eosinophil counts and increased serum immunoglobulin E (IgE) levels. Due to the heterogeneity and rarity, current diagnostic and treatment approaches for KD are often delayed and have limited efficacy.
Methods
The clinicopathological data of 51 KD patients between January 2014 and December 2023 were retrieved and stratified based on age and mass size, respectively. A case ofrecurrent KD who responded well to a low dose of mepolizumab was described.
Results
We found that pruritus occurred in 45.8% of patients, with higher rates in older patients (P=0.033). Edema was observed in 29.2%, with higher prevalence in younger patients (P=0.010). The mean maximum mass size was 3.1cm, varying by age group (P=0.005). The mean time to diagnosis was 36 months, increasing with mass size (P=0.024). Meanwhile, we reported that one of these 51 patients was successfully treated with mepolizumab at a dose of 100mg every 4 weeks.
Conclusion
We conclude that pruritus and edema are the two main clinical manifestation of KD, and the size of mass is related to the time of diagnosis. Biologic agents targeting the Th2 pathway show promise for KD treatment.
A deep learning model to diagnose and evaluate adolescent idiopathic scoliosis using biplanar radiographs
Authors: Xie Kunjie , Zhu Suping , Lin Jincong , Li Yi , Huang Jinghui , Lei Wei , Yan Yabo 
Published: October 2024

Abstract

Background
Accurate measurement of the alignment parameters of spinal radiographs is crucial for diagnosing and evaluating adolescent idiopathic scoliosis. Manual measurement is subjective and time-consuming. The recently developed artificial intelligence models mainly focused on measuring the major curve’s CA on the coronal plane and ignored the evaluation of the sagittal plane. Based on that, we developed a deep learning model that could automatically measure alignment parameters in biplanar radiographs.
Methods
In this study, our model adopted ResNet34 as the backbone network and mainly consisted of landmark detection and CA measurement. A total of 748 biplane radiographs were collected and randomly divided into training and testing sets in a 3:1 ratio. Two senior spinal surgeons independently manually measured alignment parameters and recorded the time taken. The diagnosis performance of the model was evaluated through the ROC curve and AUC. Severity classification and sagittal abnormalities were visualized using a confusion matrix. Compared with the gold standard gold, we tested the reliability and validity of the model using the ICC, simple linear regression, and Bland-Altman plots.
Results
Our AI model achieved the diagnostic accuracy of scoliosis at 97.2%, and AUC was 0.972 (0.940-1.000). For severity classification, the overall accuracy was 94.5%. All accuracy of sagittal abnormalities was greater than 91.8%. The MAD of coronal and sagittal parameters was 2.15 ° and 2.72 °, and ICC was 0.985, 0.927. The simple linear regression showed a strong correction between all parameters and the gold standard (p < 0.001, R2 > 0.686), and the Bland-Altman plots showed that the mean difference of the model was within 2 °.
Conclusions
This deep learning model can accurately and automatically measure spinal alignment parameters with reliable results, significantly reducing diagnostic time, and might provide the potential to assist clinicians.
Hepatotoxic effects of low-concentration exposure to the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) in adult male zebrafish (Danio rerio)
Authors: Oliveira Breno Raul Freitas , Neto José Soares , Davico Carla , Moreira Daniele , Pinheiro Lucas , Pereira Aline Guimarães , Izídio Geison Souza 
Published: October 2024

Abstract

2,4-Dichlorophenoxyacetic acid (2,4-D) is an herbicide widely used around the world. It has been detected in water samples, with a half-life ranging from 15 to 300 days depending on environmental conditions. This study aimed to investigate the effects of a commercial formulation containing 2,4-D on oxidative stress markers, as well as on liver histopathological and histochemical parameters, in adult male Danio rerio for 7 days. The results revealed structural and vascular lesions in the livers of zebrafish across all groups exposed to 2,4-D (at concentrations of 0.03, 0.3 and 3.0 mg/L). Analysis of the Histopathological Alteration Index suggests severe (3.0 mg/L) or moderate (0.03 and 0.3 mg/L) liver impairment in zebrafish exposed to 2,4-D. Exposure to the herbicide also led to a reduction in acid polysaccharides (0.03 and 3.0 mg/L) and glutathione (GSH) levels (at concentrations of 0.03 and 3.0 mg/L), and increased levels of the oxidized glutathione (GSSG) (at concentrations of 0.03 and 0.3 mg/L). No significant changes in lipid peroxidation levels were observed. These findings suggest that as little as 7 days of exposure to permissible concentrations of 2,4-D (0.03 mg/L) or higher (0.3 and 3.0 mg/L) can negatively affect biochemical, histochemical, and histopathological parameters, as well as the integrated biomarker response index in the liver of adult zebrafish. This study provides the first evidence of the hepatotoxic effects of the herbicide 2,4-D in adult male Danio rerio.
Explaining the experience of telephone cardiopulmonary resuscitation in Kermanshah emergency medical Dispatch: A qualitative study
Authors: zaheri Fatemeh , Abdi Alireza , Rahmati Mahmood 
Published: October 2024

Abstract

Background: Telephone Cardio Pulmonary Resuscitation (T-CPR) administered by Emergency Medical Dispatchers (EMDs) to bystanders of out-of-hospital arrest patients when the patient's condition is not visible, present significant challenges. There is limited literature on T-CPR, prompting our study to explore the experiences of T-CPR in the Kermanshah EMDs.
Method: This descriptive phenomenological study involved 12 EMDs from the Kermanshah Emergency Medical Center, all of the participants were female and with an average age of 29 years. Data were collected through in-depth, semi-structured interviews with participants selected via purposive sampling. The data were analyzed thematically using Collizi's seven-step approach.
Result: The study included 12 EMDs, with an average age of 28.33 years and 2.33 years of work experience. Analysis of 12 interviews yielded 456 codes, which were categorized into three main themes: 1-“Perceived Nursing Care in Telephone Resuscitation”: sub-themes include Nursing care in anxiety, empathic care, Care based on professional ethical principles, 2-“Professional Abilities of EMDs”: Sub-themes include perceived professional skills of EMDs and satisfaction from successful performance, 3-“Cultural Necessities Related to Telephone Resuscitation”: Sub-themes include challenges related to training, communication barriers, the need for public education and cultural changes.
Conclusion:Participants reported that T-CPR is often unsuccessful in their region due to factors such as low levels of education, the need for cultural reform, and a general lack of public awareness about emergency tasks. Effective T-CPR requires improved communication skills from EMDs, correcting the misconception that CPR can worsen the condition, presence of EMDs fluent in different accents, informing the public about answering correctly to the EMD’s questions, in strategies for managing stressful situations, and enhanced training for bystanders. Addressing these issue through better education and cultural shifts is essential for improving T-CPR outcomes.
Diagnostic Accuracy of miRNA to Identify Colorectal cancer: A Systematic Review and Meta-Analysis
Authors: Chen Zhigang , Wu Zhengheng , Tan Haifen , Yu Fuqian , Wang Dongmei 
Published: October 2024

Abstract

Background
Colorectal cancer (CRC) is a highly aggressive, high-incidence malignancy. CRC accounted for approximately one out of every ten cancer cases and deaths. Although miRNAs are often used for medical diagnostic purposes, their diagnostic effectiveness in CRC remains uncertain.
Methods
Therefore, from January 2016 to April 2024, we conducted a comprehensive search of China National Knowledge Internet (CNKI), PubMed, Cochrane Library, Web of Science (WoS) and other resources. The pooled sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic odds ratio (DOR), area under the curve (AUC) and Fagan plot analysis were used to assess the overall test performance of machine learning approaches. Moreover, we evaluated the publication bias by the Deeks’funnel plot asymmetry test.
Results
Ultimately, a total of 23 publications were identified and incorporated into this meta-analysis. The aggregated diagnostic data were as follows: The sensitivity of the test was 0.83, with a 95% confidence interval of 0.81–0.84. The specificity was found to be 0.83 with a 95% confidence interval (CI) of 0.81–0.84. The PLR was 4.60 with a 95% CI of 3.77–5.62. The NLR was 0.22 with a 95% CI of 0.17–0.27. The DOR was 23.79 with a 95% CI of 16.26–34.81. The AUC was 0.90 with a 95% CI of 0.87–0.92. The Deek funnel plot suggests that publication bias has no statistical significance. The Fagan plot analysis that the positive probability is 50% and the nagative probability is 5%.
Conclusion
In summary, our results suggest the high accuracy of miRNAs in diagnosing CRC.