bluuebunny/crossref_metadata_2025_split huggingface dataset:
Peritectic titanium alloys for 3D printing
Authors: Barriobero-Vila Pere , Gussone Joachim , Stark Andreas , Schell Norbert , Haubrich Jan , Requena Guillermo 
Published: August 2018

AbstractMetal-based additive manufacturing (AM) permits layer-by-layer fabrication of near net-shaped metallic components with complex geometries not achievable using the design constraints of traditional manufacturing. Production savings of titanium-based components by AM are estimated up to 50% owing to the current exorbitant loss of material during machining. Nowadays, most of the titanium alloys for AM are based on conventional compositions still tailored to conventional manufacturing not considering the directional thermal gradient that provokes epitaxial growth during AM. This results in severely textured microstructures associated with anisotropic structural properties usually remaining upon post-AM processing. The present investigations reveal a promising solidification and cooling path for α formation not yet exploited, in which α does not inherit the usual crystallographic orientation relationship with the parent β phase. The associated decrease in anisotropy, accompanied by the formation of equiaxed microstructures represents a step forward toward a next generation of titanium alloys for AM.
Human neuroepithelial stem cell regional specificity enables spinal cord repair through a relay circuit
Authors: Dell’Anno Maria Teresa , Wang Xingxing , Onorati Marco , Li Mingfeng , Talpo Francesca , Sekine Yuichi , Ma Shaojie , Liu Fuchen , Cafferty William B. J. , Sestan Nenad , Strittmatter Stephen M. 
Published: August 2018

AbstractTraumatic spinal cord injury results in persistent disability due to disconnection of surviving neural elements. Neural stem cell transplantation has been proposed as a therapeutic option, but optimal cell type and mechanistic aspects remain poorly defined. Here, we describe robust engraftment into lesioned immunodeficient mice of human neuroepithelial stem cells derived from the developing spinal cord and maintained in self-renewing adherent conditions for long periods. Extensive elongation of both graft and host axons occurs. Improved functional recovery after transplantation depends on neural relay function through the grafted neurons, requires the matching of neural identity to the anatomical site of injury, and is accompanied by expression of specific marker proteins. Thus, human neuroepithelial stem cells may provide an anatomically specific relay function for spinal cord injury recovery.
Reciprocal control of excitatory synapse numbers by Wnt and Wnt inhibitor PRR7 secreted on exosomes
Authors: Lee Sang H. , Shin Seung Min , Zhong Peng , Kim Hyun-Taek , Kim Dong-Il , Kim June Myoung , Heo Won Do , Kim Dae-Won , Yeo Chang-Yeol , Kim Cheol-Hee , Liu Qing-song 
Published: August 2018

AbstractSecreted Wnts play crucial roles in synaptogenesis and synapse maintenance, but endogenous factors promoting synapse elimination in central neurons remain unknown. Here we show that proline-rich 7 (PRR7) induces specific removal of excitatory synapses and acts as a Wnt inhibitor. Remarkably, transmembrane protein PRR7 is activity-dependently released by neurons via exosomes. Exosomal PRR7 is uptaken by neurons through membrane fusion and eliminates excitatory synapses in neighboring neurons. Conversely, PRR7 knockdown in sparse neurons greatly increases excitatory synapse numbers in all surrounding neurons. These non-cell autonomous effects of PRR7 are effectively negated by augmentation or blockade of Wnt signaling. PRR7 exerts its effect by blocking the exosomal secretion of Wnts, activation of GSK3β, and promoting proteasomal degradation of PSD proteins. These data uncover a proximity-dependent, reciprocal mechanism for the regulation of excitatory synapse numbers in local neurons and demonstrate the significance of exosomes in inter-neuronal signaling in the vertebrate brain.
Chitin-based barrier immunity and its loss predated mucus-colonization by indigenous gut microbiota
Authors: Nakashima Keisuke , Kimura Satoshi , Ogawa Yu , Watanabe Soichi , Soma Satoshi , Kaneko Toyoji , Yamada Lixy , Sawada Hitoshi , Tung Che-Huang , Lu Tsai-Ming , Yu Jr-Kai , Villar-Briones Alejandro , Kikuchi Sakura , Satoh Noriyuki 
Published: August 2018

AbstractMammalian gut microbiota are integral to host health. However, how this association began remains unclear. We show that in basal chordates the gut space is radially compartmentalized into a luminal part where food microbes pass and an almost axenic peripheral part, defined by membranous delamination of the gut epithelium. While this membrane, framed with chitin nanofibers, structurally resembles invertebrate peritrophic membranes, proteome supports its affinity to mammalian mucus layers, where gut microbiota colonize. In ray-finned fish, intestines harbor indigenous microbes, but chitinous membranes segregate these luminal microbes from the surrounding mucus layer. These data suggest that chitin-based barrier immunity is an ancient system, the loss of which, at least in mammals, provided mucus layers as a novel niche for microbial colonization. These findings provide a missing link for intestinal immune systems in animals, revealing disparate mucosal environment in model organisms and highlighting the loss of a proven system as innovation.
Metamaterials with amplitude gaps for elastic solitons
Authors: Deng Bolei , Wang Pai , He Qi , Tournat Vincent , Bertoldi Katia 
Published: August 2018

AbstractWe combine experimental, numerical, and analytical tools to design highly nonlinear mechanical metamaterials that exhibit a new phenomenon: gaps in amplitude for elastic vector solitons (i.e., ranges in amplitude where elastic soliton propagation is forbidden). Such gaps are fundamentally different from the spectral gaps in frequency typically observed in linear phononic crystals and acoustic metamaterials and are induced by the lack of strong coupling between the two polarizations of the vector soliton. We show that the amplitude gaps are a robust feature of our system and that their width can be controlled both by varying the structural properties of the units and by breaking the symmetry in the underlying geometry. Moreover, we demonstrate that amplitude gaps provide new opportunities to manipulate highly nonlinear elastic pulses, as demonstrated by the designed soliton splitters and diodes.
NF-κB inhibition rescues cardiac function by remodeling calcium genes in a Duchenne muscular dystrophy model
Authors: Peterson Jennifer M. , Wang David J. , Shettigar Vikram , Roof Steve R. , Canan Benjamin D. , Bakkar Nadine , Shintaku Jonathan , Gu Jin-Mo , Little Sean C. , Ratnam Nivedita M. , Londhe Priya , Lu Leina , Gaw Christopher E. , Petrosino Jennifer M. , Liyanarachchi Sandya , Wang Huating , Janssen Paul M. L. , Davis Jonathan P. , Ziolo Mark T. , Sharma Sudarshana M. , Guttridge Denis C. 
Published: August 2018

AbstractDuchenne muscular dystrophy (DMD) is a neuromuscular disorder causing progressive muscle degeneration. Although cardiomyopathy is a leading mortality cause in DMD patients, the mechanisms underlying heart failure are not well understood. Previously, we showed that NF-κB exacerbates DMD skeletal muscle pathology by promoting inflammation and impairing new muscle growth. Here, we show that NF-κB is activated in murine dystrophic (mdx) hearts, and that cardiomyocyte ablation of NF-κB rescues cardiac function. This physiological improvement is associated with a signature of upregulated calcium genes, coinciding with global enrichment of permissive H3K27 acetylation chromatin marks and depletion of the transcriptional repressors CCCTC-binding factor, SIN3 transcription regulator family member A, and histone deacetylase 1. In this respect, in DMD hearts, NF-κB acts differently from its established role as a transcriptional activator, instead promoting global changes in the chromatin landscape to regulate calcium genes and cardiac function.
A molecular overlayer with the Fibonacci square grid structure
Authors: Coates Sam , Smerdon Joseph A. , McGrath Ronan , Sharma Hem Raj 
Published: August 2018

AbstractQuasicrystals differ from conventional crystals and amorphous materials in that they possess long-range order without periodicity. They exhibit orders of rotational symmetry which are forbidden in periodic crystals, such as five-, ten-, and twelve-fold, and their structures can be described with complex aperiodic tilings such as Penrose tilings and Stampfli–Gaehler tilings. Previous theoretical work explored the structure and properties of a hypothetical four-fold symmetric quasicrystal—the so-called Fibonacci square grid. Here, we show an experimental realisation of the Fibonacci square grid structure in a molecular overlayer. Scanning tunnelling microscopy reveals that fullerenes (C60) deposited on the two-fold surface of an icosahedral Al–Pd–Mn quasicrystal selectively adsorb atop Mn atoms, forming a Fibonacci square grid. The site-specific adsorption behaviour offers the potential to generate relatively simple quasicrystalline overlayer structures with tunable physical properties and demonstrates the use of molecules as a surface chemical probe to identify atomic species on similar metallic alloy surfaces.
Amplification of chirality in surface-confined supramolecular bilayers
Authors: Cao Hai , De Feyter Steven 
Published: August 2018

AbstractOne of the most dramatic effects of supramolecular assembly is the generation of homochirality in near-racemic systems. It is normally infeasible though to flip the absolute chirality of a molecule. Here we rationalize this seemingly contradictory chiral amplification mechanism with a combined scanning tunneling microscopy (STM) and modeling study of surface-grown enantiomerically unbalanced supramolecular bilayers. We identify a chemical equilibrium between opposite but not mirror-image-related twisting molecular geometries of the pure enantiomer, and accordingly two competing aggregation pathways. The nonlinear chiral amplification effect in bilayers of near-racemic mixtures involves the biased adsorption and organization of the majority enantiomer, and the compliance of the minority enantiomer to adopt an energetically less favorable twisting molecular conformation and handed organization. By establishing a direct link between molecular building block architectures and chiral amplification effect, this study provides a general approach to gain insight into cooperative supramolecular assembly in mixed enantiomer systems.
Holocene centennial to millennial shifts in North-Atlantic storminess and ocean dynamics
Authors: Goslin Jérôme , Fruergaard Mikkel , Sander Lasse , Gałka Mariusz , Menviel Laurie , Monkenbusch Johannes , Thibault Nicolas , Clemmensen Lars B. 
Published: August 2018

AbstractThe forcing mechanisms responsible for centennial to millennial variability of mid-latitude storminess are still poorly understood. On decadal scales, the present-day geographic variability of North-Atlantic storminess responds to latitudinal shifts of the North-Atlantic westerly wind-belt under the prime control of the North-Atlantic Oscillation (NAO). An equivalent mechanism operating at centennial to millennial time scales during the Holocene is still to be ascertained, especially owing to the lack of high-resolution and continuous records of past-storminess extending far enough in time. Here we present a reconstruction of past storminess activity based on a high-resolution record of wind-blown sand retrieved from a near-coastal wetland. Our record extends back to ca. 10,000 B.P. and allows to continuously document fluctuations in the frequency of Holocene storm-force winds at our study-site at a mean high temporal resolution of 40 years. Large similarities between our record and palaeo-oceanographic records of Holocene climate changes in the North-Atlantic suggest that our past-storminess record reproduces a signal of significance for the North-Eastern Atlantic realm. We find that Holocene North-Atlantic storminess is dominated by robust millennial (≈2,200-year) to centennial (≈450, 300 and 200-year) periodicities. These changes in storminess were accompanied by changes in the precipitation regimes over northern Europe, evidencing large-scale shifts in the latitudinal positions of the Atlantic westerlies akin to present-day NAO patterns. We propose that these shifts originate from changes in the position and extent of the Azores high-pressure system and Polar vortex, as supported by climate model simulations. Finally, we demonstrate that enhanced zonal storminess activity over the North-Atlantic was the driver of millennial and centennial-scale changes in North-Atlantic oceanic circulation, while ocean dynamics most likely influenced back the atmospheric circulation at millennial time-scales. This may vouch for the instrumental role played by North-Atlantic storminess in triggering abrupt climate change at centennial scales during the Holocene.
Parallel vs. comparative evaluation of alternative options by colonies and individuals of the ant Temnothorax rugatulus
Authors: Sasaki Takao , Pratt Stephen C. , Kacelnik Alex 
Published: August 2018

AbstractBoth a single ant and the colony to which it belongs can make decisions, but the underlying mechanisms may differ. Colonies are known to be less susceptible than lone ants to “choice overload”, whereby decision quality deteriorates with increasing number of options. We probed the basis of this difference, using the model system of nest-site selection by the ant Temnothorax rugatulus. We tested the applicability of two competing models originally developed to explain information-processing mechanisms in vertebrates. The Tug of War model states that concurrent alternatives are directly compared, so that choosing between two alternatives takes longer than accepting a single one. In contrast, the Sequential Choice Model assumes that options are examined in parallel, and action takes place once any option reaches a decision criterion, so that adding more options shortens time to act. We found that single ants matched the Tug of War model while colonies fitted the Sequential Choice model. Our study shows that algorithmic models for decision-making can serve to investigate vastly different domains, from vertebrate individuals to both individuals and colonies of social insects.
Peace of mind and anxiety in the waking state are related to the affective content of dreams
Authors: Sikka Pilleriin , Pesonen Henri , Revonsuo Antti 
Published: August 2018

AbstractWaking mental well-being is assumed to be tightly linked to sleep and the affective content of dreams. However, empirical research is scant and has mostly focused on ill-being by studying the dreams of people with psychopathology. We explored the relationship between waking well-being and dream affect by measuring not only symptoms of ill-being but also different types and components of well-being. Importantly, this is the first time peace of mind was investigated as a distinct aspect of well-being in a Western sample and in relation to dream content. Healthy participants completed a well-being questionnaire, followed by a three-week daily dream diary and ratings of dream affect. Multilevel analyses showed that peace of mind was related to positive dream affect, whereas symptoms of anxiety were related to negative dream affect. Moreover, waking measures were better related to affect expressed in dream reports rather than participants’ self-ratings of dream affect. We propose that whereas anxiety may reflect affect dysregulation in waking and dreaming, peace of mind reflects enhanced affect regulation in both states of consciousness. Therefore, dream reports may possibly serve as markers of mental health. Finally, our study shows that peace of mind complements existing conceptualizations and measures of well-being.
Pan-cancer analysis of neoepitopes
Authors: Teku Gabriel N. , Vihinen Mauno 
Published: August 2018

AbstractSomatic variations are frequent and important drivers in cancers. Amino acid substitutions can yield neoantigens that are detected by the immune system. Neoantigens can lead to immune response and tumor rejection. Although neoantigen load and occurrence have been widely studied, a detailed pan-cancer analysis of the occurrence and characterization of neoepitopes is missing. We investigated the proteome-wide amino acid substitutions in 8-, 9-, 10-, and 11-mer peptides in 30 cancer types with the NetMHC 4.0 software. 11,316,078 (0.24%) of the predicted 8-, 9-, 10-, and 11-mer peptides were highly likely neoepitope candidates and were derived from 95.44% of human proteins. Binding affinity to MHC molecules is just one of the many epitope features. The most likely epitopes are those which are detected by several MHCs and of several peptide lengths. 9-mer peptides are the most common among the high binding neoantigens. 0.17% of all variants yield more than 100 neoepitopes and are considered as the best candidates for any application. Amino acid distributions indicate that variants at all positions in neoepitopes of any length are, on average, more hydrophobic than the wild-type residues. We characterized properties of neoepitopes in 30 cancer types and estimated the likely numbers of tumor-derived epitopes that could induce an immune response. We found that amino acid distributions, at all positions in neoepitopes of all lengths, contain more hydrophobic residues than the wild-type sequences implying that the hydropathy nature of neoepitopes is an important property. The neoepitope characteristics can be employed for various applications including targeted cancer vaccine development for precision medicine.
Shifting patterns of seasonal influenza epidemics
Authors: Coletti Pietro , Poletto Chiara , Turbelin Clément , Blanchon Thierry , Colizza Vittoria 
Published: August 2018

AbstractSeasonal waves of influenza display a complex spatiotemporal pattern resulting from the interplay of biological, sociodemographic, and environmental factors. At country level many studies characterized the robust properties of annual epidemics, depicting a typical season. Here we analyzed season-by-season variability, introducing a clustering approach to assess the deviations from typical spreading patterns. The classification is performed on the similarity of temporal configurations of onset and peak times of regional epidemics, based on influenza-like-illness time-series in France from 1984 to 2014. We observed a larger variability in the onset compared to the peak. Two relevant classes of clusters emerge: groups of seasons sharing similar recurrent spreading patterns (clustered seasons) and single seasons displaying unique patterns (monoids). Recurrent patterns exhibit a more pronounced spatial signature than unique patterns. We assessed how seasons shift between these classes from onset to peak depending on epidemiological, environmental, and socio-demographic variables. We found that the spatial dynamics of influenza and its association with commuting, previously observed as a general property of French influenza epidemics, apply only to seasons exhibiting recurrent patterns. The proposed methodology is successful in providing new insights on influenza spread and can be applied to incidence time-series of different countries and different diseases.
Cytochrome C oxidase Inhibition and Cold Plasma-derived Oxidants Synergize in Melanoma Cell Death Induction
Authors: Gandhirajan Rajesh Kumar , Rödder Katrin , Bodnar Yana , Pasqual-Melo Gabriella , Emmert Steffen , Griguer Corinne E. , Weltmann Klaus-Dieter , Bekeschus Sander 
Published: August 2018

AbstractDespite striking advances in the treatment of metastasized melanoma, the disease is often still fatal. Attention is therefore paid towards combinational regimens. Oxidants endogenously produced in mitochondria are currently targeted in pre-clinical and clinical studies. Cytotoxic synergism of mitochondrial cytochrome c oxidase (CcO) inhibition in conjunction with addition of exogenous oxidants in 2D and 3D melanoma cell culture models were examined. Murine (B16) and human SK-MEL-28 melanoma cells exposed to low-dose CcO inhibitors (potassium cyanide or sodium azide) or exogenous oxidants alone were non-toxic. However, we identified a potent cytotoxic synergism upon CcO inhibition and plasma-derived oxidants that led to rapid onset of caspase-independent melanoma cell death. This was mediated by mitochondrial dysfunction induced by superoxide elevation and ATP depletion. This observation was validated by siRNA-mediated knockdown of COX4I1 in SK-MEL-28 cells with cytotoxicity in the presence of exogenous oxidants. Similar effects were obtained with ADDA 5, a recently identified specific inhibitor of CcO activity showing low toxicity in vivo. Human keratinocytes were not affected by this combinational treatment, suggesting selective effects on melanoma cells. Hence, targeting mitochondrial CcO activity in conjunction with exogenous pro oxidant therapies may constitute a new and effective melanoma treatment modality.
Polymorphisms in the Toll-like receptor 3 (TLR3) gene are associated with the natural course of hepatitis B virus infection in Caucasian population
Authors: Fischer Janett , Koukoulioti Eleni , Schott Eckart , Fülöp Balazs , Heyne Renate , Berg Thomas , van Bömmel Florian 
Published: August 2018

AbstractInnate immunity can induce spontaneous hepatitis B surface antigen (HBsAg) seroclearance (SC) of hepatitis B virus (HBV) infection or transition towards an inactive carrier state. Toll-like receptor (TLR) 3 signalling has been linked to these processes. Alterations in the TLR3 gene might impair immune responses against HBV. In our study, we analysed the impact of the TLR3 polymorphisms rs3775291 and rs5743305 on the natural course of HBV infection. In this retrospective study, a Caucasian cohort of 621 patients with chronic HBV infection (CHB), 239 individuals with spontaneous HBsAg SC, and 254 healthy controls were enrolled. In the CHB group, 49% of patients were inactive carriers, and 17% were HBeAg-positive. The TLR3 rs3775291 A allele was associated with a reduced likelihood of spontaneous HBsAg SC and HBeAg SC, and an increased risk of developing chronic hepatitis B. In haplotype analysis, the haplotype including both risk variants rs3775291A and rs5743305A had the lowest likelihood of HBsAg SC. Further research in larger cohorts and functional analyses are needed to shed light on the impact of TLR3 signalling.
Novel genome and genome-wide SNPs reveal early fragmentation effects in an edge-tolerant songbird population across an urbanized tropical metropolis
Authors: Tan David J. X. , Chattopadhyay Balaji , Garg Kritika M. , Cros Emilie , Ericson Per G. P. , Irestedt Martin , Rheindt Frank E. 
Published: August 2018

AbstractAlthough edge-tolerant species are known to benefit from habitat fragmentation, less is known about the population genetic impacts fragmentation may exert on edge-tolerant species. We examined the landscape genomic structure of an edge-tolerant forest-dependent bird species, the Striped Tit-BabblerMixornis gularis, in the heavily urbanized island of Singapore to determine if two centuries of fragmentation have led to signs of isolation and loss of population-genetic diversity in different parts of the island. We obtained a high-quality complete reference genome with 78x coverage. Using almost 4000 SNPs from double-digest RAD-Sequencing across 46 individuals, we found that the population has likely experienced a recent contraction in effective population size and presently exhibits low population genetic diversity. Using empirical and simulation-based landscape genomic analyses, we also found that the subtle population genetic structure observed in the Striped Tit-Babbler population in Singapore is likely driven by isolation by distance resulting from limited dispersal. Our results demonstrate that population genetic impoverishment and subdivision can accumulate at relatively rapid rates in edge-tolerant bird species such as the Striped Tit-Babbler as a result of fragmentation, and that subtle spatial genetic structure can be detected over fine spatial and temporal scales using relatively few multilocus genomic SNPs.