bluuebunny/crossref_metadata_2025_split huggingface dataset:
Methadone-Induced Encephalopathy: A Case Series and Literature Review
Authors: Haghighi-Morad Maryam , Naseri Zahra , Jamshidi Nazila , Hassanian-Moghaddam Hossein , Zamani Nasim , Ahmad-Molaei Leila 
Published: October 2019

Abstract
Background: Accidental ingestion or consumption of supra-therapeutic doses of methadone can result in neurological sequelae in humans. We aimed to determine the neurological deficits of methadone-poisoned patients admitted to a referral poisoning hospital using the brain magnetic resonance (MR) and diffusion weighted (DW) imaging.Methods: In this retrospective study, brain MRIs of the patients admitted to our referral center due to methadone intoxication were reviewed. Methadone intoxication was confirmed based on history, congruent clinical presentation, and confirmatory urine analysis tests. Each patient had an MRI with Echo planar T1, T2, FLAIR, and DWI and apparent deficient coefficient (ADC) sequences without contrast media. Abnormalities were recorded and categorized based on their anatomic location and sequence. Results: Ten patients with abnormal MRI findings were identified. Eight had acute- and two had delayed-onset encephalopathy. Imaging findings included bilateral confluent or patchy T2 and FLAIR high signal intensity in cerebral white matter, cerebellar involvement, and bilateral occipito-parietal cortex diffusion restriction in DWI. Internal capsule involvement was identified in two patients while abnormality in globus pallidus and head of caudate nuclei were reported in another one. Bilateral cerebral symmetrical confluent white matter signal abnormality with sparing of subcortical U-fibers on T2 and FLAIR sequences were observed in both patients with delayed-onset encephalopathy.Conclusions: Acute- and delayed-onset encephalopathies are two rare adverse events detected in methadone-intoxicated patients. Brain MRI findings can be helpful in detection of methadone-induced encephalopathy.
The relationship between metabolic syndrome and increased risk of Barrett's esophagus: An Updated systematic review and meta-analysis
Authors: Karimian Mohammad , Salamati Majid , Azami Milad 
Published: October 2019

Abstract
Background: The relationship between MetS and Barrett's esophagus (BE) is still a challenging issue, and inconsistent results have been reported in different studies. Therefore, this study was conducted to determine the relationship between MetS and BE. Methods: In this study, we followed the MOOSE protocol and the PRISMA guidelines for reporting the results. All study steps were performed independently by two authors. If necessary, the dispute was resolved by consultation with a third author. The search strategy is designed to find published studies. Comprehensive search was done in the following biomedical databases until July 2019: Cochrane Library, PubMed/Medline, Web of Science, Science Direct, EMBASE, Scopus, CINAHL, EBSCO, and Google Scholar search engine. All analyses were performed using Comprehensive Meta-Analysis Software Ver.2, while p-value lower than 0.05 was considered significant. Results: In 14 studies with a sample size of 108416, MetS significantly increased the risk of BE (OR=1.315; 95% CI: 1.110-1.558; P<0.001; Heterogeneity: I 2 = 81.55; P<0.001). Sensitivity analysis by omitting one study showed that overall estimates are still robust. Subgroup analysis was significant for continent (P<0.001) and MetS diagnostic criteria (P=0.043), but was not significant for variables of study type (P=0.899), study setting (P=0.115), control groups (P=0.671) and quality of studies (P=0.603). The Begg (P=0.912) and Egger's (P=0.094) tests were not significant; therefore, the publication bias did not play a role in the results. Conclusion: MetS increases the risk of BE compared to control groups. Future studies should examine whether treatment for metabolic syndrome reduces the risk of BE.
Self-Analysis of Repeat Proteins Reveals Evolutionarily Conserved Patterns
Authors: Merski Matthew , Młynarczyk Krzysztof , Ludwiczak Jan , Skrzeczkowski Jakub , Dunin-Horkawicz Stanisław , Górna Maria W. 
Published: October 2019

Abstract
Background Protein repeats can confound sequence analyses due to the repetitiveness of their amino acid sequences leading to difficulties in identifying when similar repeats are due to convergent or divergent evolution. We noted that the patterns derived from traditional dot plot protein self-analysis tended to be conserved in sets of related repeat proteins and this conservation could be quantified using a standard Jaccard metric.Results Use of these plots obviated the issues due to sequence similarity for analysis of these proteins. The dot plot patterns decay quickly in the absence of selective pressure with an expected loss of 50% of Jaccard similarity due to a loss of 8.2% sequence identity. Comparison of repeat and non-repeat proteins in the PDB suggested that the information content in dot plots could be used to identify repeat proteins from pure sequence. Analysis of the UniProt90 database suggested that 16.9% of all known proteins could be classified as repeat proteins. These 13.3 million putative repeat protein chains were clustered and significant amount (82.9%) of clusters containing between 5 and 200 members were of a single functional type.Conclusions Dot plot analysis of repeat proteins obviates the issues that arise from sequence degeneracy. These results show that this kind of analysis can efficiently be applied to analyze repeat proteins on a large scale.
Validation and evaluation of common biomarker in human cancers sera protein detected by a monoclonal antibody UNIVMab
Authors: Dandi Manjunath , Kumaraswamy Sunil B , V Shashidhara A , Appaiah Hitesh N , Thomas Anil , BANERJEE SHIBDAS 
Published: October 2019

Abstract
Objective: Management and diagnosis of multiple human cancers remain a challenge and search for a common biomarker are still debatable. We described a method and evaluated the use of monoclonal antibody UNIVMab, to detect the protein (H11) as a common biomarker for all cancer irrespective of grade and origin . H11 protein identified as a unique Hyaluronan binding protein not detected earlier. We applied this test both with ELISA, Western blot, fractionated in anion exchange, cibacron gel exclusion, b-Hyaluronan interaction and HA-Oligo competition from various grades of Human cancers sera and processed for the detection of hyaluronan binding protein H11, reacted with Monoclonal antibody UNIVMab and with b-HA. Results: Studies from ELISA, Western blot and b-Hyaluronan interactions showed a definitive positive over-expression of UNIVMab reacted H11 antigen in all fortyfive cancer sera compared to normal sera and can be used as a common biomarker. We believe, UNIVMab detected H11 antigen, a unique hyaluronan binding protein, is a common biomarker for all cancer sera. Keywords: ELISA, Western blot, Hyaluronic acid binding protein, H11 (sera antigen), UNIVMab. Common Biomarker, Cancers Sera
Forecasting incidence of infectious diarrhea using Random forest in Jiangsu province, China
Authors: Fang Xinyu , Liu Wendong , Ai Jing , Wu Ying , Shi Yingying , Shen Wenqi , Bao Changjun 
Published: October 2019

Abstract
Background: Infectious diarrhea caused considerable disease burden around the world. An accurate forecast of infectious diarrhea epidemic based on predictive models is crucial for public health authorities. We aimed to develop an optimal random forest（RF）model to predict infectious diarrhea incidence using meteorological factors in Jiangsu province, China. Methods: A RF model was constructed to predict infectious diarrhea incidence in Jiangsu province base on the morbidity during 2012-2017 and synchronous meteorological data, then compared with classical ARIMA and ARIMAX model to evaluate the model prediction effect. Results: The RF model was established including atmosphere pressure, precipitation, relative humidity and their lagged terms, one to four weeks lagged morbidity and time variable as predictors. Meanwhile, a univariate model ARIMA(1,0,1)(1,0,0) 52 (AIC=-575.92, BIC=-558.14) and a multivariable model ARIMAX(1,0,1)(1,0,0) 52 with precipitation and its one-week lagged term as external variables (AIC=-578.58, BIC=-578.13) were developed as benchmark models. Predictive values of RF model matched the real morbidity very well, with a mean absolute percentage (MAPE) about 20%. The performance of the multivariate ARIMAX model was similar to that of the univariate ARIMA model. Both of them performed much worse than the RF model, with a MAPE as high as near to 30%. Conclusions: In brief, RF model well integrated autocorrelation and seasonality of morbidity. It also comprehensively combined meteorological factors and their hysteresis effects. This model well fitted the dynamic of infectious diarrhea epidemic and achieved ideal predication accuracy. It could be used to predict the epidemic level, and has a good prospect of practical application.
Transcriptome Analysis of Photosynthetic Characteristics was Induced by Low Temperature Stress in Brassica napus L.
Authors: Jiaojiao Jin , Zigang Liu , Wenbo Mi , Wancang Sun , Junyan Wu , Yan Fang , Xuecai Li , Lijun Liu , Ya Zou , Mingxia Xu , Chunmei Xu 
Published: October 2019

Abstract
Background RNA Sequencing (RNA-Seq) technique could be utilized to compare the transcription groups of two different cold-resistant rapeseed leaves responding to low temperature at the seedling stage, analyze the photosynthetic characteristics of rapeseed subjected to low temperature stress, and identify the related genes for low temperature induction in rapeseed leaves. Results Using cold-tolerant variety 17NS and sensitive variety NF24 as experimental materials, carrying out RNA-Seq analysis by photosynthetic parameter determination and Illumina HiSeqTM platform. and screen out the KEGG significant enrichment pathway related to photosynthetic characteristics under low temperature stress. Differential Expressed Genes (DEGs) were used for real-time PCR to verify the reliability of RNA-Seq results. The results showed that the response of Brassica napus L. to low temperature stress mainly was achieved by inhibiting photosynthesis, the cold-tolerant variety 17NS had a strong ability to maintain membrane system stability and structural integrity after 24 h of low temperature stress, while the sensitive variety NF24 photosynthesis was significantly inhibited. Two pathways of Photosynthesis and Photosynthesis-antennas, which were significantly correlated with photosynthetic characteristics and low temperature stress were screened by KEGG enrichment. The results of DEGs indicated that 64 differentially expressed genes in these two pathways were induced by low temperature stress, and 8 of them were up-regulated expression and 56 of them were down-regulated expression. The expression pattern of DEGs was consistent with the results of RNA-Seq analysis by qRT-PCR detection and confirmed the reliability of RNA-Seq results. Conclusion Our study analysis and identified 17 low-temperature-induced photosynthetic-related candidate genes in Brassica napus L., and the GO and KEGG metabolic pathways clarified the molecular function of differentially expressed genes.
Quality of water sources in Southwestern Uganda using the compartment bag test (CBT): a cross-sectional descriptive study
Authors: Apecu Richard Onyuthi , Ampaire Lucas , Mulogo Edgar Mugema , Bagenda Fred Norman , Traore Afsatou , Potgieter Natasha 
Published: October 2019

AbstractThe aim of this study was to assess the bacteriological quality of water sources in the two rural areas of Uganda using the compartment bag test (CBT). In total, 200 water samples were collected from 69 different water sources and processed within 6 h of collection. Positive and negative controls were processed each day together with water samples. Physical parameters were measured in situ. Descriptive statistics were used to generate mean, minimum, maximum, standard deviations and percentages. The results indicated that 29% of the water sources met the National Standards and World Health Organization (WHO) Guidelines for drinking water. Sixty percent of the borehole, 44% of gravitational flow taps and 14% of roof rain water met the required standards. Of the open water sources, 75% of the rivers, 50% of open channels and 43% of unprotected dug wells plus 25% of protected springs and 9% of gravitational flow schemes had most probable number counts &gt;100 Escherichia coli/100 mL of water. Most of the water sources in the study areas were not fit for human consumption without prior treatment. The CBT was found to be robust and easy to use in all field situations. The mean physical parameters of water sources were within the acceptable limits.
Soil erosion in disturbed forests and agricultural plantations in tropical undulating terrain: in situ measurement using a laser erosion bridge method
Authors: Mohamad N. A. , Nainar A. , Annammala K. V. , Sugumaran D. , Jamal M. H. , Yusop Z. 
Published: October 2019

Abstract
The rapid growth of agricultural plantations and climatic extremes has raised concerns pertaining to enhanced soil erosion. Soil erosion studies are still relatively limited in Malaysia. In this study, soil erosion in four sites such as high conservation value forests (HCVFs), logged forest (LF), mature oil palm (MOP), and mature rubber (MR) within the Kelantan River Basin was measured. A total of 3,207 measurements were conducted via the Modified Laser Erosion Bridge in all sites over 1 year. Results of soil erosion are 87.63, 25.45, 8.44, and 5.90 t ha−1 yr−1 for the HCVF, LF, MOP and MR, respectively – classified as very severe (HCVF), very high (LF), moderate (MP) and slight (MR) according to the Indian condition classification. Steep slope gradient (significant positive correlation to erosion) and logging are the main factors attributed to the high erosion rates. This is to be further explored in the future and more detailed studies should be conducted on the HCVF and LF areas, respectively. Mitigation measures and sustainable agricultural practices should be planned to control and reduce soil erosion.
Energy consumption in a baffled membrane bioreactor (B-MBR): estimation based on long-term continuous operation
Authors: Miyoshi T. , Nguyen T. P. , Tsumuraya T. , Kimura K. , Watanabe Y. 
Published: October 2019

Abstract
We investigated the operating conditions of a baffled membrane bioreactor (B-MBR) under which long-term stable operation can be achieved through the continuous operation of a pilot-scale B-MBR. Under appropriate operating conditions, the B-MBR was capable of achieving excellent treated water quality in terms of biochemical oxygen demand and concentration of total nitrogen. Excellent removal of total phosphorus was also achieved. In addition, the degree of membrane fouling was acceptable, indicating that stable continuous operation of a B-MBR is possible under the operating conditions adopted in the present study. Estimation of the specific energy consumption in hypothetical full-scale B-MBRs operated under the conditions recommended by the findings was also performed in this study. The results suggest that energy consumption in full-scale B-MBRs would be in the range of 0.20–0.22 kWh/m3. These results strongly suggest that energy consumption in MBR operation can be significantly reduced by applying the concept of a B-MBR.
Role of Ischemia Modified Albumin Serum Levels as an Oxidative Stress Marker in Children with Diabetic Ketoacidosis
Authors: Yazıcı Mutlu U. , Ayar Ganime , Savas-Erdeve Senay , Azapağası Ebru , Neşelioğlu Salim , Erel Özcan , Çetinkaya Semra 
Published: October 2019

Aim and Objective:
Ischemia modified albumin (IMA) is a biomarker that has been introduced
recently for use in the evaluation of oxidative stress. The aim of this study was to measure the ischemia
modified albumin serum levels in pediatric patients with diabetic ketoacidosis (DKA) during acidosis and
after the patient recovered from acidosis and to compare these with the control group.

Materials and Methods:
Pediatric patients with Type I diabetes mellitus (T1DM) who were admitted
to the pediatric intensive care unit with the diabetic ketoacidosis were assigned as the study group and
healthy children who were admitted to the outpatient clinic and decided as healthy after clinic and
laboratory evaluation were selected as the control group. IMA and adjusted IMA levels were evaluated
in the blood samples from the control group and the study group when admitted first time to the
intensive care unit during the acidosis period (DKA before treatment, DKA-BT), and after recovering
from acidosis (DKA after treatment, DKA-AT).

Results:
A total of 24 pediatric patients with diabetic ketoacidosis and 30 healthy control children
matching age and sex were included in the current study. The albumin levels in pediatric patients with
T1DM during DKA-BT were higher than the albumin levels after acidosis (4.101±0.373, 3.854±0.369
g/dL, respectively) (p<0.05). However, there was no significant difference when these values were
compared to the control group. Mean values of IMA and Adj-IMA were statistically higher in DKAAT
compared to the control group (0.748±0.150 vs 0.591±0.099, p< 0.001; 0.708±0.125 vs
0.607±0.824, p< 0.001, respectively). IMA and adjusted IMA levels measured after recovered from
acidosis were significantly higher compared to the level of IMA during DKA (0.748±0.150 vs
0.606±0.105 as absorbance unit, p<0.001; 0.708±0.125 vs 0.625±0.100, p<0.05, respectively).

Conclusion:
In children with T1DM, even though acidosis recovered following the treatment in
diabetic ketoacidosis, which is an oxidative stress marker, the ischemia modified albumin levels and
adjusted ischemia modified albumin levels were high.
Change in Quality of Life of OSAHS Patients with Minimally Invasive Surgery or CPAP Therapy: A 2-year Retrospective, Single-center Parallel-group Study
Authors: Zhang Xiao-Qing , Zhao Xin , Hong Pei-Wei , Zhou Jin , Zeng Ping , Liu Cong , Li Xiao-ying , Zhao Yang , Jiang Li-Qiong 
Published: October 2019

Background:
By including untreated obstructive sleep apnea-hypopnea syndrome
(OSAHS) patients as the control group, this study explores the influence of minimally invasive
surgical treatment and continuous positive airway pressure (CPAP) therapy on OSAHS patients,
with the subjective and objective performance. The study also discusses their relationship,
determines the effect factor, and provides a simple and practical method for evaluation of clinical
efficacy.

Methods:
A total of 90 OSAHS patients, who were diagnosed in the Sleep Disorders Diagnosis and
Treatment Center of Sichuan Province from May 2014 to May 2016, were selected for the present
study. These patients were divided into three groups: surgery group, CPAP group, and untreated
group. These patients were followed up at six months, one year, and two years, respectively. The
physiological indicators, clinical symptoms, degree of daytime sleepiness and quality of life were
compared among these three groups. The daytime sleepiness and the quality of life before and
after minimally invasive surgery and CPAP treatment were evaluated, and the subjective and
objective efficacy of surgery and CPAP treatment was explored.

Results:
Among these 90 patients, 11 (12.2%) patients had hypertension, while two (2.2%) patients had
diabetes. The average AHI score was 50.53±23.39 per hour, and the mean minimum oxygen saturation
and mean oxygen saturation was 71.25±14.16% and 90.13±5.90%, respectively. There were statistically
significant differences in mouth breathing, morning sore throat and daytime sleepiness in the group
having received surgery at 0.5 year and one year. In the CPAP group, there were statistically significant
differences in mouth breathing, morning sore throat and daytime sleepiness at 0.5 year, one year and two
years. Moreover, there were statistically significant differences in memory loss at one year and two years,
and there were statistically significant differences in frequent nocturia at one year. The ESS value in the
surgery group decreased at 0.5 year and one year, but increased at two years. The situation was the
same in terms of the total points and in each dimension of the SF-36 paramter. The delta values of ESS
among the three groups had statistical significance at 0.5 year, one year and two years, in which the
CPAP group experienced the most changes, followed by the surgery group and the group received
health education.

Conclusions:
For minimally invasive surgery, CPAP therapy and health education can improve
daytime sleepiness and quality of life. CPAP therapy was found to be the most effective, followed
by minimally invasive surgery and provision of health education. However, the treatment of OSAHS
should be comprehensive.
Identification of Novel Sesamol Dimers with Unusual Methylenedioxy Ring-Opening Skeleton and Evaluation of Their Antioxidant and Cytotoxic Activities
Authors: Murthy Sudtha , Hazli Ummi H.A.M. , Kong Kin W. , Mai Chun-Wai , Leong Chee-Onn , Rahman Noorsaadah A. , Lo Kong M. , Chee Chin F. 
Published: October 2019

Background:
Sesamol is a widely used antioxidant for the food and pharmaceutical industries. The
oxidation products of this compound may be accumulated in foods or ingested. Little is known about its effect
on human health.

Objective:
It is of great interest to identify the oxidation products of sesamol that may be beneficial to humans.
This study was undertaken to identify the oxidation products of sesamol and investigate their antioxidant and
cytotoxic activities.

Materials and Methods:
Using the ferricyanide oxidation approach, four oxidation products of sesamol (2, 3,
20 & 21) have been identified. Structural elucidation of these compounds was established on the basis of their
detailed NMR spectroscopic analysis, mass spectrometry and x-ray crystallography. Additionally, a formation
mechanism of compound 20 was proposed based on high-resolution mass spectrometry-fragmentation method.
The antioxidant activities of these compounds were determined by the DPPH, FRAP, and ABTS assays. The
in vitro antiproliferative activity of these compounds was evaluated against a panel of human cancer cell lines
as well as non-cancerous cells.

Results:
Two oxidation products of sesamol were found to contain an unusual methylenedioxy ring-opening
skeleton, as evidenced by spectroscopic and x-ray crystallographic data. Among all compounds, 20 displayed
impressive antiproliferative activities against a panel of human cancer cell lines yet remained non-toxic to noncancerous
cells. The antioxidant activities of compound 20 are significantly weaker than sesamol as determined
by the DPPH, FRAP, and ABTS assays.

Conclusion::
The oxidation products of sesamol could be a valuable source of bioactive molecules. Compound
20 may be used as a potential lead molecule for cancer studies.