bluuebunny/crossref_metadata_2025_split huggingface dataset:
TIPOLOGIA DO CONSUMIDOR DE FRUTOS DO CERRADO
Authors: Soares Camila Mariane da Silva , Aguiar Aynaran Oliveira de , Silva Romilda Ramos da , Ibiapina Andreia , Santos André Leonardo dos , Martins Glêndara Aparecida de Souza 
Published: October 2019

O Cerrado é um bioma altamente diversificado, rico em frutas que possuem sabor sui-generis, podendo ser consumidas in natura ou processada. A maioria desses frutos são ricos em vitaminas, antocianinas, flavonóides e possuem alto potencial antioxidante. No entanto ainda há pouco conhecimento da população quanto aos benefícios proporcionados pelo consumo, potencial econômico e aplicação tecnológica desses frutos. Com base nisso, essa pesquisa foi desenvolvida com o intuito de fazer um levantamento sobre o conhecimento da população em relação aos frutos nativos do Cerrado, além de verificar o perfil dos consumidores e o conhecimento sobre os benefícios desses frutos. Foram entrevistadas 352 pessoas de todos os estados brasileiros, que responderam a um questionário online. Constatou-se, que 91% possui conhecimento quanto aos frutos, no entanto 55,3% não tinham ciência quanto aos benefícios de consumo e 12,2% nunca consumiram, mesmo os residentes das regiões do Norte e Nordeste, que abrangem o bioma do Cerrado. Concluiu-se que a maioria dos consumidores de frutos do cerrado são mulheres, sendo cajuí, buriti e murici os frutos mais populares, por já estrem inclusos na alimentação da maioria dos entrevistados. No entanto ainda é vasto o desconhecimento dos consumidores quanto a diversidade dos frutos e potencial de processamento.
Palavras-chave: Aplicação tecnológica; Benefícios; Pesquisa exploratória;
Pengembangan LKS Pintar Elektronik (Lekasparkin) Untuk Meningkatkan Minat Belajar Siswa SMA
Authors: Ardiani Fatmala Nur , Suharno Suharno , Musadad Akhmad Arif 
Published: October 2019

Penelitian ini bertujuan untuk (1) Menjelaskan LKS yang digunakan dalam pembelajaran di SMA 2 Bhineka Karya Boyolali, (2) mendeskripsikan langkah-langkah pengembangan LKS Pintar Elektronik, (3) memaparkan hasil pengembangan LKS Pintar Elektronik, dan (4) menguji efektifitas LKS Pintar Elektronik yang dihasilkan. Jenis penelitian ini adalah penelitian pengembangan (R&amp;D). Model penelitian pengembangan yang digunakan adalah model ADDIE yang terdiri dari (1) Analysis,menganalisis materi materi dan media yang dibutuhkan dalam pembelajaran (2) Design, mendesain draf produk LKS Pintar Elektronik dalam pembelajaran, (3) Development, Mengembangkan media LKS Pintar Elektronik dalam pembelajaran (4) Implementation LKS Pintar Elektronik media dan materi penjasorkes (5) Evaluatin. Subyek penelitian ini adalah siswa kelas XI SMA 2 Bhineka Karya Boyolali mapel penjasorkes. Teknik pengumpulan data yang digunakan adalah observasi, wawancara, dokumentasi, dan lembar penilaian. Hasil penilaian lembar penilaian dari ahli media memperoleh hasil rata-rata 85%, hasil dari rata-rata ahli materi 85,25%, uji coba satusatu 85,75%, hasil uji coba kelompok kecil tahap I 77,88%, uji coba kelompok kecil tahap II adalah 83% dan uji coba lapangan 83%. Hasil dari kelas eksperimen memperoleh nilai rata-rata 82.88, sementara kelas kontrol memperoleh nilai rata-rata 68.00 ayat atau setara dengan 2,8 halaman. Hasil uji efektifitas produk dengan nilai signifikansi 5% diperoleh nilai sig. (2-tailed) 0,000&lt;0,05. Kesimpulan penelitian ini adalah Lembar Kerja Elektronik efektif dapat meningkatkan minat belajar siswa
Contaminating reactivity of a monoclonal CCAAT/Enhancer Binding Protein β antibody in differentiating myoblasts
Authors: AlSudais Hamood , Wiper-Bergeron Nadine 
Published: October 2019

Abstract
Objective CCAAT/Enhancer Binding proteins (C/EBPs) are transcription factors involved in the regulation of a variety of cellular processes. We used the Abcam Recombinant Anti-C/EBP beta antibody (E299) to detect C/EBPβ expression during myogenesis. Though the antibody is monoclonal, and the immunogen used is highly specific to C/EBPβ, we identified an intense band at 23 kDa on western blot that did not correspond to any of the known isoforms of C/EBPβ, or family members predicted to cross-react. Absent in myoblast cells overexpressing C/EBPβ, the band was present when C/EBPβ was knocked down, confirming specificity for a protein other than C/EBPβ. The objective of this work was to identify the contaminating reactivity. Results We performed immunoprecipitation followed by mass spectrometry to identified myosin light chain 4 (MYL4) as the unknown band, suggesting that the Abcam monoclonal antibody directed against C/EBPβ is not pure, but contains a contaminating antibody against MYL4. Caution should be used when working in cells lines that express MYL4 to not confound the detection of MYL4 with that of the C/EBPβ LIP isoform.
Later Onset of Hypertension Increased the Risk of Dementia in Mild Cognitive Impairment
Authors: Author) hongyun qin(Former Corresponding , Hu chengping , Wang ling , Guo yi , Cao Zhicheng , Zhao xudong , Author) binggen Zhu(New Corresponding 
Published: October 2019

Abstract
Objective

To investigate the effect of hypertension development on the progression of MCI for middle aged and elderly people.

Methods

A population-based longitudinal cognition survey of people aged 55+ was conducted. The hypertension onset age was estimated by self-reported information and medical insurance card records. In order to study the effect of later onset of hypertension on dementia, the incidence of dementia was compared between the two groups.

Results

A total of 277 h ypertensive mild cognitive impairment (MCI) participants without dementia were followed for 6 years. Overall, 56 MCI participants (20.22%) progressed to dementia (MCIp). The proportion of MCIp participants in the old-age onset hypertension group (≥ 65 years) was higher than that of middle-age onset one (27.0% vs 15.4%; X 2 = 5.538, P = 0.019). And in the old-age onset hypertension group, the proportion of MCIp without diabetes mellitus was higher than those with diabetes mellitus (24.7% vs 12.6%; X 2 = 5.321, P = 0.021), and the proportion of MCIp with increased pulse pressure was higher than those without increased pulse pressure (33.3% vs 15.4%; X 2 = 3.902, P = 0.048). However, the multivariate logistic regression analysis showed that older age was the only risk for MCIp (OR=0.732, p=0.029).

Conclusions

These results suggest that, later onset hypertension was harmful to cognition even in cases where the level of blood pressure maintained at 130/80 mmHg with antihypertensive management.
Hydrogen sulfide induced by hydrogen peroxide mediates darkness-induced stomatal closure in Arabidopsis thaliana
Authors: Ma Yinli , Shao Luhan , Niu Jiao 
Published: October 2019

Abstract
Background Whether stomatal movement by darkness in Arabidopsis thaliana is mediated by hydrogen sulfide (H2S) is undiscovered yet, so the interaction between hydrogen peroxide (H2O2) and H2S in the process needs to be elucidated.
Results Our results indicated that H2S modulators aminooxy acetic acid (AOA), potassium pyruvate (N3H3KO3) + ammonia (NH3), hydroxylamine (NH2OH), and hypotaurine (HT) inhibited darkness-induced stomatal closure, H2S generation and L-/D-cysteine desulfhydrase (L-/D-CDes) activity increased in wild-type A. thaliana leaves. Darkness induced stomatal closure in wild-type plants, but failed in Atl-cdes and Atd-cdes mutants. Additionally, both L-/D-CDes activity and H2S content were significantly decreased after applying H2O2 modulators salicylhydroxamic acid (SHAM), ascorbic acid (ASA), diphenylene iodonium (DPI), and catalase (CAT) in darkness, but there was almost no effects on H2O2 levels in the presence of AOA, C3H3KO3+NH3, NH2OH, and HT of wild-type plants in darkness. Moreover, darkness couldn't increase H2S content and L-/D-CDes activity of AtrbohF and AtrbohD/F mutants leaves, but increased H2O2 levels in Atl-cdes and Atd-cdes guard cells.
Conclusions We observed that L-/D-CDes-generated H2S mediates stomatal closure by darkness, and functions downstream of H2O2 in A. thaliana.
Effectiveness of a health intervention based on WHO food safety manual in Iran
Authors: Ghaffari Mohtasham , Mehrabi Yadollah , Rakhshanderou Sakineh , Safari-Moradabadi Ali , Jafarian Seyyede Zenab 
Published: October 2019

Abstract
Background: Food safety manual was developed by the World Health Organization (WHO) to train professionals to reduce the burden of foodborne diseases as a global strategy. The present pioneering research aimed to explore the effectiveness of an intervention based on the manual of five keys to safer food by WHO in enhancing the knowledge, attitude and behavior of Iranian health volunteers. Methods:In the present experimental research, health volunteers (n=125) were selected and assigned to two groups, an intervention and a control. A modified version of the questionnaire based on WHO manual was used to measure knowledge, attitude and behavior of the sample. The questionnaire was first completed at the outset of the study (pre-test) and then once again in two months of the intervention (post-test). Face and content validity of the questionnaire was tested and confirmed. Cronbach’s alpha was used to test the reliability of the questionnaire along with the test-retest method of testing reliability. The data entered SPSS16 for statistical analysis. To this aim, Chi-squared test, dependent and independent samples T-test, ANOVA and ANCOVA were run. Results:The two groups showed no statistically significant difference in the pretest (p>.05). In the post-test, the mean scores for all variables was higher in the intervention group than the control, and this difference between the two research groups was statistically significant (p<.001). When the volunteers were adjusted for age and experience in healthcare centers, the mean scores were significantly higher in the intervention group than the control (p<.001). Conclusion: It was revealed in the present study that the educational intervention based on five keys to food safety manual by WHO managed to improve participants’ knowledge, attitude and behavior. Translation of the target guideline in future can be a great help to researchers in prospective research.
Higher education among the “forgotten half”: The association between substance use and college completion among 2-year college students versus matched 4-year college students from a nationally representative longitudinal study
Authors: Rosenbaum Janet E. 
Published: October 2019

Abstract
Background: Recent policy changes have increased marijuana availability to college students. Past research has not evaluated the association between substance use and college completion among the forgotten half of disadvantaged young adults who access higher education through 2-year colleges. Methods: This study uses a subsample of college-enrolled participants from the National Longitudinal Study of Adolescent and Adult Health to evaluate whether substance use in 2001 among students attending 2-year college and 4-year college predicts educational attainment in 2008. We used Mahalanobis nearest-neighbor and exact matching within propensity score calipers to identify a comparison group of 4-year college students (n=888) similar to the 2-year college students (n=1398) on 15 baseline measures including grades, test scores, and substance use. We used multivariate regression in the matched sample using a Poisson working model to estimate the relative risk of earning no post-secondary degree.Results: Compared with matched 4-year college students, 2-year college students were more likely to use methamphetamines, cocaine, marijuana, and more likely to report problematic substance use, and less likely to use alcohol. Two-year college students who used methamphetamines in the past year (IRR=1.51, 95% CI (1.12, 2.04), p=0.007) or past month (IRR=1.69, 95% CI (1.09, 2.61), p=0.02), or completed alcohol abuse treatment (IRR=1.58, 95% CI (1.21, 2.07), p<0.001) were less likely to complete college. Four-year college students who reported that drugs interfered with school or work in the past year (IRR=1.84 (1.28, 2.64), p=0.001), used cocaine in the past year (IRR=1.47 (1.04, 2.08), p=0.03), and used marijuana in the past year (IRR=1.30 (1.07, 1.57), p=0.007), past month (IRR=1.31 (1.07, 1.61), p=0.01), or ≥5 times in the past month (IRR=1.44 (1.12, 1.85) p=0.005) were less likely to complete college. Conclusions: Substance use interventions should target 2-year and 4-year college students. Two-year colleges that better accommodate students who complete substance use treatment may improve these students’ completion. Students who use marijuana or cocaine or whose drug use impairs functioning may benefit from an incremental approach of completing a 2-year degree prior to transfer for a 4-year degree, rather than enrolling directly in a 4-year program.
In vivo and in vitro study on characterization and mechanism of the intestinal absorption of 2,3,5,4'-tetrahydroxy-stilbene-2-O-β-D-glucoside
Authors: Wang Cheng , Zhou Yimeng , Gong Xiaohong , Zheng Li , Li Yunxia 
Published: October 2019

Abstract
Background: 2,3,5,4'-tetrahydroxystilbence-2-O-β-D-glucoside(TSG) is a polyhydroxyphenolic compound, which exhibits a broad spectrum of pharmacological activities, such asanti-inflammatory, anti-depression, anti-oxidation and anti-atherosclerosis.However, the compound has poor bioavailability and the underlying absorption mechanisms has not been studied. Therefore, the purpose of this study was to investigate the intestinal absorption mechanism of TSG. Methods: This study used the Caco-2 cell monolayer model and the single-passintestinal perfusion modelto explore the intestinal absorption mechanisms of TSG. The effects of basic parameters such as drug concentration, time and pH on the intestinal absorption of TSG were analyzed by high performance liquid chromatography.In addition, the susceptibility of TSG absorption process to treatment with three inhibitors, such as P-gp inhibitors verapamil hydrochloride and quinidine, and the MRP2 inhibitor probenecid were also assessed. Results: TSG is poorly absorbed in the intestines and the absorption of TSG in the stomach is much higher than that in the intestine. Both in vivo and in vitro experiments showed that the absorption of TSG was saturated with increasing concentration. and it was better absorbed in a weakly acidic environment with a pH of 6.4. Moreover, TSG interacts with P-gp and MRP2, and TSG is not only the substrate of the P-gp and MRP2, but also affects the expression of P-gp and MRP2. Conclusions: It can be concluded that the intestinal absorption mechanismsofTSG involve processes passive transport and the participation of efflux transporters.
What matters to me – A web-based preference elicitation tool for clients in long-term care: a user-centred design
Authors: Leersum Catharina Margaretha van , Moser Albine , Steenkiste Ben van , Reinartz Marion , Stoffers Esther , Wolf Judith R.L.M. , Weijden Trudy van der 
Published: October 2019

Abstract
Background: During the process of decision-making for long-term care, clients are often dependent on informal support and available information about quality ratings of care services. However, clients do not take ratings into account when considering preferred care, and need assistance to understand their preferences. A tool to elicit preferences for long-term care could be beneficial. Therefore, the aim of this usability study is to understand the user requirements and develop a web-based preference elicitation tool for clients in need of long-term care. Methods: We applied a user-centred design in which end-users influence the development of the tool. The included end-users were clients, relatives, and healthcare professionals. Data collection took place between November 2017 and March 2018 by means of meetings with the development team consisting of four users, walkthrough interviews with 21 individual users, video-audio recordings, field notes, and observations during the use of the tool. Data were collected during three phases of iteration: Look and feel, Navigation, and Content. A deductive and inductive content analysis approach was used for data analysis. Results: The layout was considered accessible and easy during the Look and feel phase, and users asked for neutral images. Users found navigation easy, and expressed the need for concise and shorter text blocks. Users reached consensus about the categories of preferences, wished to adjust the content with propositions about well-being, and discussed linguistic difficulties. Conclusion: By incorporating the requirements of end-users, the user-centred design proved to be useful in progressing from the prototype to the finalized tool ‘What matters to me’. This tool may assist the elicitation of client’s preferences in their search for long-term care.
Faculty Retention in Medical Schools of Iran: A Qualitative Content Analysis
Authors: Shaterjalali Maria , Gholampoor Yousef , Jeihooni Ali Khani , Mansoori Yaser , Homayounfar Reza , Ehrampoush Elham , Karimi Shahnaz 
Published: October 2019

Abstract
Background and purpose: Faculty are the cornerstone of academic medicine and are the important assets for any institution offering higher education. The recruitment and retention of capable faculty are essential to maintain and improve the quality of education and research performance of universities.The purpose of the present study is to discover the views, experiences, and attitudes of faculty to identify the reasons for the attrition and the retention of faculty. Methods: Using the qualitative content analysis, a total of 20 transferred faculty or those who requested to be transferred participated in the study. Data were collected using semi-structured interviews in face to face and by telephone. To measure the trustworthiness of the data, we used the four components of Credibility, transferability, dependability, and confirmability proposed by Lincoln and Guba. Results: The findings were divided into 3 categories and 14 sub-categories: the first category was the retention enforcers, including 4 sub-categories of facilitated communication, distance, gaining experience, and support of authorities; the second category was the retention threats, including 6 subcategories of social infrastructures, individual dimension, occupational dimension, economic dimension, sense of respect, and executive management dimension; and the third category was the retention strategies, including 4 sub-categories of the process of recruiting and promotion, connecting with major universities, facilitating scientific growth, and fulfilling the future needs. Conclusion: Several factors play role in the retention of faculties in medical schools. Authorities can create a more positive environment by devising a suitable reward system, supporting academic activities, and increasing the level of practical autonomy of faculty, to develop a sense of belonging among the workforce, and reduce the intention to transfer of their human resources.
Use of MethHC and SurvExpress database in the course of molecular biology for medical students: Understanding of epigenetic modifications in gene expression with problem-based learning
Authors: Villarreal Joel Jimenez , Valero Silvia Selene Mercado , Urbano Iván Eleazar Cipriano , Villa Víctor Daniel Boone , Joya Jorge Alejandro Aguirre , Armendáriz Diana Isabel Rivas , Martínez Nadia Denis Betancourt , Martínez Javier Morán 
Published: October 2019

Abstract
Background: Currently, there are teaching methods that allow students to understand clinical conditions, pathologies and hereditary diseases, mainly supported by problem-based learning (PBL), simulation-based learning (SBL), image analysis, case studies, and use of platforms electronic and mobile applications. However, the use of databases and PBL in the compression of molecular biology applied to research has not been well investigated. The scientific literature has reported combinations of pedagogical strategies with positive results compared to the traditional method based on reading, therefore, this study aimed to design a pedagogic method making use of databases applied in scientific research to understand the relation of methylation on gene expression and its relationship in the appearance of diseases such as cancer. Methods: Three databases were used, Genetics Home Reference (GHR) for the theoretical understanding of the study gene, MethHC for the analysis of the effect of methylation on gene expression and SurvExpress for biomarker validation for cancer gene expression. All databases support your information from The Cancer Genome Atlas (TCGA) program of the National Cancer Institute (NCI). The guidelines of each platform for the analysis of the information were followed. The present study exemplified with the analysis of the POT1 gene that is involved with the maintenance of telomere length and has been associated with breast cancer. Results: The function of the POT1 gene, chromosomal and molecular position, number of exons and their transcriptional variants in invasive breast carcinoma were described. It was found that the expression of POT1 depends on the positioning of the levels of methylation within the structures that comprise the gene in healthy and tumor tissue, besides it was reported through a survival analysis that the gene is best expressed in the low group's risk compared to the high-risk group. Conclusion: The use of databases allows the student to make use of his theoretical knowledge and take them to clinical practice and research, the results obtained from the use of GHR, MethHC, and SurvExpress allow to apply molecular biology in clinical research and promote problem-based learning developing analysis and interpretation skills in students. This pedagogical method allows the understanding of epigenetic effects on the expression of cancer-related genes.
Reliability of residents’ assessments of their postgraduate medical education learning environment: an observational study
Authors: Brand Paul L P , Rosingh H Jeroen , Meijssen Maarten A C , Nijholt Ingrid M , Dünnwald Saskia , Prins Jelle , Schönrock-Adema Johanna 
Published: October 2019

Abstract
Background. Even in anonymous evaluations of a postgraduate medical education (PGME) program, residents may be reluctant to provide an honest evaluation of their PGME program, because they fear embarrassment or repercussions from their supervisors if their anonymity as a respondent is endangered. This study was set up to test the hypothesis that current residents in a PGME program provide more positive evaluations of their PGME program than residents having completed it. We therefore compared PGME learning environment evaluations of current residents in the program to leaving residents having completed it.Methods. This observational study used data gathered routinely in the quality cycle of PGME programs at two Dutch teaching hospitals to test our hypothesis. At both hospitals, all current PGME residents are requested to complete the Scan of Postgraduate Education Environment Domains (SPEED) annually. Residents leaving the hospital after completion of the PGME program are also asked to complete the SPEED after an exit interview with the hospital’s independent residency coordinator. All SPEED evaluations are collected and analysed anonymously. We compared the residents’ grades (on a 1-10 scale) on the three SPEED domains (content, atmosphere, and organization of the program) and their mean (overall department grade) between current and leaving residents. Results. Mean (SD) overall SPEED department grades were 8.00 (0.52) for 287 current residents in 39 PGME programs and 8.07 (0.48) for 170 leaving residents in 39 programs. Neither the overall SPEED department grades (t test, p=0.53, 95% CI for difference -0.16 to 0.31) nor the department SPEED domain grades (MANOVA, F(3, 62)=0.79, p=0.51) were significantly different between current and leaving residents. Conclusions. Residents leaving the program did not provide more critical evaluations of their PGME learning environment than current residents in the program. This suggests that current residents’ evaluations of their postgraduate learning environment were not affected by social desirability bias or fear of repercussions from faculty.