Shallow Whole Genome Sequencing of Circulating Tumor DNA Is Predictive of Outcome in Diffuse Large B-Cell Lymphoma
Authors: Zhao Davidson , Shelton Victoria , Gong Inna , Rangel-Patiño Juan , Liu Ting , Hong Michael , Murad Vanessa , Metser Ur , Prica Anca , Kukreti Vishal , Bhella Sita , Vijenthira Abi , Crump Michael , Kuruvilla John , Lam Bernard , Kridel Robert 
Published: December 2024

Introduction
Patients with diffuse large B-cell lymphoma (DLBCL) have heterogeneous outcomes, which necessitates the discovery of novel prognostic biomarkers. Plasma from peripheral blood is a minimally invasive source of circulating tumor DNA (ctDNA). Prior studies using targeted DNA sequencing have revealed that pre-treatment ctDNA levels are predictive of outcome in DLBCL (Kurtz et al. 2018, etc.). However, such assays are expensive, and there remains a need for cost-effective methods to reliably estimate tumor burden in plasma. To this end, we evaluated the clinical utility of ctDNA in patients with newly diagnosed DLBCL using shallow whole genome sequencing (sWGS) - a low-cost method to estimate tumor burden without prior knowledge of tumor mutations.
Methods
sWGS (intended coverage 0.1-1X) was performed on plasma from peripheral blood samples of patients with newly diagnosed DLBCL at our institution. ichorCNA was used to estimate tumor fraction in plasma from sWGS (Adalsteinsson et al. 2017). FDG PET-CT scan was performed for disease burden assessment. Targeted DNA sequencing (hybridization capture) for recurrently mutated genes in lymphoma was performed on plasma samples; ctDNA concentration was expressed as log haploid genome equivalents per mL of plasma. The Fisher's exact test, Mann Whitney test and Pearson's correlation were used as appropriate to assess association between variables.
Results
We performed sWGS on pre-treatment plasma samples from 53 patients with newly diagnosed DLBCL (median age 62 years). The median follow-up time of the cohort was 2.6 years (range: 0.02 - 4.8 years). The majority (47/53, 89%) of patients were treated with curative-intent chemoimmunotherapy regimens including R-CHOP (n=42), R-CHOP/HD-MTX (n=4) and DA-EPOCH-R (n=1). FDG PET-CT data from time of diagnosis was available for 50 (94%) patients.
High tumor fraction, as stratified by the median value (0.07, range: 0 - 0.61), in pre-treatment plasma was associated with established measures of tumor burden, including advanced-stage disease (Ann Arbor stage III/IV; 69% vs. 22%; P &lt; 0.001), elevated serum lactate dehydrogenase (median 351 vs. 250 U/L; P &lt; 0.001), increased tumor size (maximum diameter; median 7 vs. 4 cm; P &lt; 0.001), and &gt;1 extranodal site (38% vs. 7%; P = 0.009). Tumor fraction in pre-treatment plasma was correlated with total lesion glycolysis assessed by FDG PET-CT (R = 0.51, P &lt; 0.001).
Targeted DNA sequencing was performed in pre-treatment plasma samples for 50 (94%) patients, of which the majority (47/50, 94%) had detectable mutation(s) in plasma. Tumor fraction quantified using sWGS was concordant with ctDNA concentration as determined by targeted DNA sequencing (R = 0.75, P &lt; 0.001).
Among patients treated with curative-intent chemoimmunotherapy regimens, high tumor fraction in pre-treatment plasma, as determined by sWGS, was associated with inferior progression-free survival (PFS, 2-year PFS 53% vs. 94%, P &lt; 0.001) and overall survival (OS, 2-year OS 60% vs. 97%, P = 0.003). In multivariable analysis, high cell-free tumor fraction remained a predictor for inferior PFS but not OS (PFS: P = 0.037, OS: P = 0.102), independent of International Prognostic Index scoring and total lesion glycolysis.
Conclusions
Tumor fraction in plasma quantified using sWGS is correlated with established measures of tumor burden in DLBCL and orthogonal measures of tumor burden in plasma. Importantly, high tumor fraction is associated with inferior outcomes in patients with newly diagnosed DLBCL. Our study highlights the potential clinical utility of sWGS performed on ctDNA as a minimally invasive assay for DLBCL risk assessment.