bluuebunny/crossref_metadata_2025_split huggingface dataset:
Concurrent live imaging of DNA double-strand break repair and cell-cycle progression by CRISPR/Cas9-mediated knock-in of a tricistronic vector
Authors: Otsuka Kensuke , Tomita Masanori 
Published: November 2018

AbstractCell-cycle progression can be arrested by ionizing radiation-induced DNA double-strand breaks (DSBs). Although DSBs are patched by DSB repair systems, which comprise proteins such as p53-binding protein 1 (53BP1), the relationship between DSB repair progression and cell-cycle status in living cells is unclear. The probe FUCCI (fluorescent ubiquitination-based cell-cycle indicator) was previously developed for visualizing cell-cycle status. Here, we established novel live-imaging probes based on custom-designed plasmids designated “Focicles” harboring a tricistronic compartment encoding distinct fluorescent proteins ligated to the murine 53BP1 foci-forming region (FFR) and two cell-cycle indicators that are known components of FUCCI (hCdt1 and hGmnn). We used CRISPR/Cas9-mediated genome editing to obtain Focicle knock-in cell lines in NIH3T3 cells, which were subject to X-ray irradiation that induced comparable numbers of Focicle and endogenous-53BP1 foci. In addition, the Focicle probes enabled the kinetic analysis of both DSB repair and cell-cycle arrest/progression after irradiation, demonstrating that the Focicle knock-in cells progressed to cell division after DNA damage elimination. These newly developed probes can help to gain a better understanding of the dynamics of DSB repair and cell-cycle control to in turn guide cancer treatment development and cancer-risk assessments.
Investigating the Neurological Correlates of Workplace Deviance Using a Rodent Model of Extinction
Authors: Tabor J. , Griep Y. , Collins R. , Mychasiuk R. 
Published: November 2018

AbstractEmployee deviance and time theft is an expensive and pervasive workplace problem. Research indicates that a primary reason employees engage in deviant behaviour is the perception of injustice often associated with psychological contract breach (i.e., broken promises). This study used a rodent model to mimic said experience of broken promises and then examined the subsequent neurophysiological changes that lead to the display of deviant behaviours. Specifically, we generated a psychological contract using a 3 choice serial reaction task, then broke the promise, and finally examined deviant behaviours and neurological correlates. After the broken promise, rats had elevated levels of corticosterone and testosterone, engaged in riskier behaviour, and were more aggressive. The most prominent changes in gene expression were associated with serotonin and stress, and were found in the nucleus accumbens. This study highlights the value of pre-clinical models in the investigation of the theoretical tenants of industrial and organizational psychology.
The utility of urinary CD80 as a diagnostic marker in patients with renal diseases
Authors: Minamikawa Shogo , Nozu Kandai , Maeta Shingo , Yamamura Tomohiko , Nakanishi Keita , Fujimura Junya , Horinouchi Tomoko , Nagano China , Sakakibara Nana , Nagase Hiroaki , Shima Hideaki , Noda Kenta , Ninchoji Takeshi , Kaito Hiroshi , Iijima Kazumoto 
Published: November 2018

AbstractCD80, which regulates T cell activation, may provide a differential diagnostic marker between minimal change disease (MCD) and other renal diseases, including focal segmental glomerular sclerosis (FSGS). However, recent reports show contrasting results. Therefore, we evaluated the utility of urinary CD80 as a diagnostic biomarker. We collected 65 urine samples from 55 patients with MCD (n = 31), FSGS (n = 4), inherited nephrotic syndrome (n = 4), Alport syndrome (n = 5) and other glomerular diseases (n = 11), and control samples (n = 30). We measured urinary CD80 levels by ELISA. Urinary CD80 (ng/gCr) (median, interquartile range) levels were significantly higher in patients with MCD in relapse (91.5, 31.1–356.0), FSGS (376.2, 62.7–1916.0), and inherited nephrotic syndrome (220.1, 62.9–865.3), than in patients with MCD in remission (29.5, 21.7–52.8) (p < 0.05). Elevation of urinary CD80 was observed, even in patients with inherited nephrotic syndrome unrelated to T cell activation. Additionally, urinary CD80 was positively correlated with urinary protein levels. Our results suggest that urinary CD80 is unreliable as a differential diagnostic marker between MCD in relapse and FSGS or inherited kidney diseases. Increased urinary CD80 excretion was present in all patients with active kidney disease.
Stories From the Webcams: Cincinnati Children's Hospital Medical Center Audiology Telehealth and Pediatric Auditory Device Services
Authors: Steuerwald Wendy , Windmill Ian , Scott Michael , Evans Thomas , Kramer Kathleen 
Published: November 2018

Purpose
The purpose of this manuscript is to describe the regulatory, technological, and training considerations for audiologists investigating telehealth and to offer some examples of audiology services provided through telehealth.

Method
The authors presented the regulatory components, the technology required for audiology staff and patients, and staff training for the audiology telehealth program at Cincinnati Children's Hospital Medical Center. Four case studies highlighting the successful use of telehealth in providing auditory device services to patients were also presented.

Results and Conclusion
The described regulatory, technological, and training hierarchy provides a framework for audiologists interested in starting a telehealth program. The cases presented illustrate that telehealth can be used to provide some auditory device services, such as troubleshooting, mapping, and parent consulting.
Findings and Ethical Considerations From a Thematic Analysis of Threads Within Tinnitus Online Support Groups
Authors: Ainscough Eve , Smith Sandra N. , Greenwell Kate , Hoare Derek J. 
Published: November 2018

Purpose
Tinnitus is the perception of noise without a corresponding external stimulus. Current management typically aims to moderate associated psychosocial stressors and allow sufferers to retain an adequate quality of life. With the increasing recognition of the Internet as a repository for health advice, information, and support, the online support group has become a popular coping strategy for those living with chronic conditions such as tinnitus. Patients find that communicating with each other, providing encouragement, and sharing information in the absence of physical and temporal boundaries are invaluable ways of managing their condition. The purpose of this study was to explore the potential positive and negative consequences of participating in online support groups for tinnitus.

Method
Discussion forum threads were collated from across 4 public online support group websites. All threads were initiated between February and April 2016. Texts from these threads were coded by 3 separate analysts using both inductive and deductive thematic analysis, until data saturation was reached.

Results
Analysis of 75 threads (641 individual posts) found 9 independent themes pertaining to aspects of participation in tinnitus online support groups. The results revealed that using the forums allowed users to exchange knowledge and experiences, express complex emotions, profit from a network of support, and engage in everyday conversation away from the burden of their tinnitus. However, some experiences appeared to be compromised by negative messages, limited communication, and informational issues such as conflicting advice or information overload.

Conclusions
This study represents the 1st research into discussion forums in tinnitus online support groups. A nonintrusive (passive) analysis method was used, whereby messages comprising the dataset were retrieved without direct interaction with the discussion forum. Individuals and the community of tinnitus online support groups are deemed to be at low risk from potential harm in this study. Most tinnitus patients likely benefit from accessing online support groups, for example, they discover they are not alone, and they find new coping strategies. However, for those who are particularly vulnerable or prone to psychological stress, accessing these groups could be detrimental.
Innovation in the Context of Audiology and in the Context of the Internet
Authors: Bernstein Lynne E. , Besser Jana , Maidment David W. , Swanepoel De Wet 
Published: November 2018

Purpose
This article explores different meanings of innovation within the context of audiology and the Internet. Case studies are used to illustrate and elaborate on the new types of innovation and their levels of impact.

Method
The article defines innovation, providing case studies illustrating a taxonomy of innovation types.

Results
Innovation ranges from minor changes in technology implemented on existing platforms to radical or disruptive changes that provide exceptional benefits and transform markets. Innovations within the context of audiology and the Internet can be found across that range. The case studies presented demonstrate that innovations in hearing care can span across a number of innovation types and levels of impact. Considering the global need for improved access and efficiency in hearing care, innovations that demonstrate a sustainable impact on a large scale, with the potential to rapidly upscale this impact, should be prioritized.

Conclusions
It is unclear presently what types of innovations are likely to have the most profound impacts on audiology in the coming years. In the best case, they will lead to more efficient, effective, and widespread availability of hearing health on a global scale.
Enriching Communicative Environments: Leveraging Advances in Neuroplasticity for Improving Outcomes in Neurogenic Communication Disorders
Authors: Hengst Julie A. , Duff Melissa C. , Jones Theresa A. 
Published: November 2018

PurposeResearch manipulating the complexity of housing environments for healthy and brain-damaged animals has offered strong, well-replicated evidence for the positive impacts in animal models ofenriched environmentson neuroplasticity and behavioral outcomes across the lifespan. This article reviews foundational work on environmental enrichment from the animal literature and considers how it relates to a line of research examiningrich communicative environmentsamong adults with aphasia, amnesia, and related cognitive-communication disorders.MethodDrawing on the authors' own research and the broader literature, this article first presents a critical review ofenvironmental complexityfrom the animal literature. Building on that animal research, the second section begins by definingrich communicative environmentsfor humans (highlighting the combined effects of complexity, voluntariness, and experiential quality). It then introduces key frameworks for analyzing and designing rich communicative environments:distributed communicationandfunctional systemsalong with sociocultural theories of learning and development in humans that support them. The final section provides an overview of Hengst's and Duff's basic and translational research, which has been designed to exploit the insights of sociocultural theories and research on environmental complexity. In particular, this research has aimed to enrich communicative interactions in clinical settings, to trace specific communicative resources that characterize such interactions, and to marshal rich communicative environments for therapeutic goals for individuals with aphasia and amnesia.ConclusionsThis article concludes by arguing that enriching and optimizing environments and experiences offers a very promising approach to rehabilitation efforts designed to enhance the reorganization of cognitive-communicative abilities after brain injury. Such interventions would require clinicians to use the principles outlined here to enrich communicative environments and to target distributed communication in functional systems (not the isolated language of individuals).
Short-Term Intensive Therapy and Outcomes for Athletes With Paradoxical Vocal Fold Motion Disorder
Authors: Gallena Sally K. , Johnson Arthur T. , Vossoughi Jafar 
Published: November 2018

Purpose
The aim of the study was to develop a treatment for athletes with paradoxical vocal fold motion disorder (PVFMD) based on exercise physiology and learning theory principles and administer it over a preestablished time frame.

Method

A prospective, repeated-measures, within-subject group design was used. Eleven adolescent/teen athletes diagnosed with PVFMD via laryngoscopy received short-term intensive (STI) therapy. Eight of the athletes returned for extended follow-up. Changes in postexercise inspiratory (
R
i
) and expiratory (
R
e
) resistances and Modified Borg Dyspnea Scale (MBDS) ratings collected at baseline were compared immediately posttreatment and at extended follow-up. Dyspnea Index scores were collected at baseline and at extended follow-up. Two no-treatment control athletes with PVFMD participated in two exercise challenges—baseline and 6 weeks later.

Results

Immediately after STI therapy, athletes attained significant improvement in
R
i
,
R
e
, and MBDS ratings. These changes were maintained at extended follow-up as well as a significant change in Dyspnea Index scores. The 2 control athletes who were reassessed 6 weeks after baseline experienced negative changes in postexercise
R
i
and MBDS ratings.

Conclusion
STI therapy that incorporated individuality, specificity, and variable practice effectively changed outcome measures posttreatment with further improvement observed at extended follow-up. These results provide preliminary evidence for STI therapy for PVFMD.
Sensitivity to Morphosyntactic Information in Preschool Children With and Without Developmental Language Disorder: A Follow-Up Study
Authors: Deevy Patricia , Leonard Laurence B. 
Published: November 2018

PurposeThis study tested children's sensitivity to tense/agreement information in fronted auxiliaries during online comprehension of questions (e.g.,Are the nice little dogs running?). Data from children with developmental language disorder (DLD) were compared to previously published data from typically developing (TD) children matched according to sentence comprehension test scores.MethodFifteen 5-year-old children with DLD and fifteen 3-year-old TD children participated in a looking-while-listening task. Children viewed pairs of pictures, 1 with a single agent and 1 with multiple agents, accompanied by a sentence with a fronted auxiliary (is+ single agent orare+ two agents) or a control sentence. Proportion looking to the target was measured.ResultsChildren with DLD did not show anticipatory looking based on the number information contained in the auxiliary (isorare) as the younger TD children had. Both groups showed significant increases in looking to the target upon hearing the subject noun (e.g.,dogs).ConclusionsDespite the groups' similar sentence comprehension abilities and ability to accurately respond to the information provided by the subject noun, children with DLD did not show sensitivity to number information on the fronted auxiliary. This insensitivity is considered in light of these children's weaker command of tense/agreement forms in their speech. Specifically, we consider the possibility that failure to grasp the relation between the subject–verb sequence (e.g.,dogs running) and preceding information (e.g.,are) in questions in the input contributes to the protracted inconsistency in producing auxiliary forms in obligatory contexts by children with DLD.Supplemental Materialhttps://doi.org/10.23641/asha.7283459
On the periodicity of linear and nonlinear oscillatory reconnection
Authors: Thurgood J. O. , Pontin D. I. , McLaughlin J. A. 
Published: November 2018

Context.An injection of energy towards a magnetic null point can drive reversals of current-sheet polarity leading to time-dependent, oscillatory reconnection (OR), which may explain periodic phenomena generated when reconnection occurs in the solar atmosphere. However, the details of what controls the period of these current-sheet oscillations in realistic systems is poorly understood, despite being of crucial importance in assessing whether a specific model of OR can account for observed periodic behaviour.Aims.This paper aims to highlight that different types of reconnection reversal are supported about null points, and that these can be distinct from the oscillation in the closed-boundary, linear systems considered by a number of authors in the 1990s. In particular, we explore the features of a nonlinear oscillation local to the null point, and examine the effect of resistivity and perturbation energy on the period, contrasting it to the linear, closed-boundary case.Methods.Numerical simulations of the single-fluid, resistive MHD equations are used to investigate the effects of plasma resistivity and perturbation energy upon the resulting OR.Results.It is found that for small perturbations that behave linearly, the inverse Lundquist number dictates the period, provided the perturbation energy (i.e. the free energy) is small relative to the inverse Lundquist number defined on the boundary, regardless of the broadband structure of the initial perturbation. However, when the perturbation energy exceeds the threshold required for “nonlinear” null collapse to occur, a complex oscillation of the magnetic field is produced which is, at most, only weakly-dependent on the resistivity. The resultant periodicity is instead strongly influenced by the amount of free energy, with more energetic perturbations producing higher-frequency oscillations.Conclusions.Crucially, with regards to typical solar-based and astrophysical-based input energies, we demonstrate that the majority far exceed the threshold for nonlinearity to develop. This substantially alters the properties and periodicity of both null collapse and subsequent OR. Therefore, nonlinear regimes of OR should be considered in solar and astrophysical contexts.
Effects of A 3-Month Physiotherapy Intervention in Stable Cervical Dystonia As Add-On to Botulinum Toxin Therapy
Authors: Werner Christian , Derlien Steffen , Best Norman , Witte Otto , Smolenski Ulrich , Günther Albrecht 
Published: November 2018

Abstract
Background Treatment of choice for cervical dystonia comprises botulinum neurotoxin therapy. Whether additional physiotherapy can achieve better results is unclear.
Objectives To show effects of physiotherapy as an add-on treatment to botulinum neurotoxin therapy.
Methods 18 patients participated in this prospective interventional study (DRKS00006562) consisting of 2, 3-month periods. In the first period, usual botulinum toxin was applied. The second period comprised an additional multimodal physiotherapy program. Outcome measures were: range of motion, SF-36, Toronto Western Spasmodic Torticollis Rating Scale (TWSTRS), surface electromyography.
Results In the comparison first phase vs. second phase patients showed significant improvements in range of motion (flexion/extension: 16.3 ± 25.4; rotation: 19.1 ± 24.6), TWSTRS (severity: 3.8 ± 3.0; disability: 1.7 ± 2.0; pain: 4.4 ± 4,1) and in mental health (SF-36: 5.9 ± 7.5); p <0.05, respectively. No significant changes were seen in the lateral flexion ROM, physical health (SF-36) and the surface electromyography.
Conclusion Multimodal physiotherapy is a feasible and save additional treatment option for patients with cervical dystonia. An add-on physiotherapy program can achieve better treatment outcomes. Patients benefited especially with improved range of motion, and reduced pain and severity of symptoms.
Linksseitiger Chylothorax und Tumor des rechten vorderen Mediastinums
Authors: Bougioukas I. , Seipelt R. , Huwer H. 
Published: November 2018

ZusammenfassungEs wird über einen 82-jährigen Patienten berichtet, der mit einem linksseitigen Chylothorax symptomatisch wurde. Die CT-Untersuchung zeigte einen Tumor im rechten vorderen oberen Mediastinum und Strukturen im linken Mediastinum, die als vergrößerte Lymphknoten interpretiert wurden. Wegen des Verdachtes auf ein Lymphom und zur Therapie des Chylothorax erfolgte eine linksseitige Thorakotomie. Die vermeintlichen Lymphknoten erwiesen sich als Chylome. Wir interpretieren den Mediastinaltumor als zystisches Lymphangiom und als Ursache des Chylothorax bei rechts mündendem Ductus thoracicus.
Comparative Pharmacokinetics of Mitragynine after Oral Administration of Mitragyna speciosa (Kratom) Leaf Extracts in Rats
Authors: Avery Bonnie , Boddu Sai , Sharma Abhisheak , Furr Edward , Leon Francisco , Cutler Stephen , McCurdy Christopher 
Published: November 2018

AbstractKratom (Mitragyna speciosa) has been examined for its opioid activity, especially for the treatment of opioid withdrawal and pain. Mitragynine, the most abundant alkaloid in kratom, is thought to be the major psychoactive alkaloid. An HPLC method was developed for the quantification of mitragynine in kratom leaf extracts. In addition, a multiple reaction mode based UPLC-MS/MS method was developed and validated for the quantification of mitragynine in rat plasma. Pharmacokinetic studies were performed by comparing a single intravenous dose of mitragynine (5 mg/kg, mitragynine hydrochloride) to a single oral dose of mitragynine (20 mg/kg, mitragynine hydrochloride), lyophilized kratom tea, and the organic fraction of the lyophilized kratom tea at an equivalent mitragynine dose of 20 mg/kg in rats. After intravenous administration, mitragynine exhibited a biexponential decrease in the concentration-time profile, indicating the fast distribution of mitragynine from the systemic circulation or central compartment to the peripheral compartments. Mitragynine hydrochloride, lyophilized kratom tea, and the lyophilized kratom tea organic fraction were dosed orally and the absolute oral bioavailability of mitragynine in rats was found to be 1.5- and 1.8-fold higher than that of mitragynine dosed alone. The results provide evidence that an equivalent oral dose of the traditional preparation (lyophilized kratom tea) and formulated/manufactured products (organic fraction) of kratom leaves provide better systemic exposure of mitragynine than that of mitragynine dosed alone.
The Actin Regulator Coronin-1A Modulates Platelet Shape Change and Consolidates Arterial Thrombosis
Authors: Stocker Thomas , Pircher Joachim , Skenderi Artid , Ehrlich Andreas , Eberle Clemens , Megens Remco , Petzold Tobias , Zhang Zhe , Walzog Barbara , Müller-Taubenberger Annette , Weber Christian , Massberg Steffen , Ishikawa-Ankerhold Hellen , Schulz Christian 
Published: November 2018

AbstractCoronin-1A (Coro1A) belongs to a family of highly conserved actin-binding proteins that regulate cytoskeletal re-arrangement. In mammalians, Coro1A expression is most abundant in the haematopoietic lineage, where it regulates various cellular processes. The role of Coro1A in platelets has been previously unknown. Here, we identified Coro1A in human and mouse platelets. Genetic absence of Coro1A in mouse platelets inhibited agonist-induced actin polymerization and altered cofilin phosphoregulation, leading to a reduction in spreading and low-dose collagen induced aggregation. Furthermore, Coro1A-deficient mice displayed a defect in ferric chloride-induced arterial thrombosis with prolonged thrombus formation and reduced thrombus size. Immunofluorescence analysis revealed a less compact thrombus structure with reduced density of platelets and fibrinogen. In summary, Coro1A has a role in platelet biology with impact on spreading, aggregation and thrombosis.