bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ultra-wideband elliptical patch antenna for microwave imaging of wood
Authors: Saeidi Tale , Ismail Idris , Wen Wong Peng , Alhawari Adam R. H. 
Published: May 2019

AbstractThis paper presents the design of an elliptical shape ultra-wide band antenna for imaging of wood. The antenna is constructed comprising an elliptical shape of patch loaded by a stub to resonate at lower bands, strip loading at the back, and chamfered ground. Despite having miniaturized dimensions of 20 mm × 20 mm, the proposed antenna shows better results compared to recent studies. The simulation results depict a good ultra-wide bandwidth from 2.68 to 16 GHz, and 18.2–20 GHz. Besides, the proposed antenna has two low-frequency bands at 0.89–0.92 and 1.52–1.62 GHz, maximum gain of 5.48 dB, and maximum directivity of 6.9 dBi. The measurement outcomes are performed in air, plywood, and high-density wood and show a good agreement with the simulated results done using electromagnetic simulator CST. In addition to that, the measurement results of S-parameters, transmitted and received signals show a good agreement with the simulated results. Besides, the measured results illustrate a good isolation and uniform illumination among arrays as well as the received signals' shapes do not change in different environments, but only the amplitude. Hence, the proposed antenna seems to be adequate for microwave imaging of wood.
More humanitarian accountability, less humanitarian access? Alternative ideas on accountability for protection activities in conflict settings
Authors: Klein-Kelly Natalie 
Published: May 2019

AbstractAmbitions to fulfil accountability demands in humanitarian action are high, including for protection activities in armed conflict settings. However, from a Dunantist position, meeting accountability demands is often not only unsatisfactory for practical reasons, but is also inappropriate in view of humanitarian principles and flawed from related ethical perspectives. Regarding accountability primarily as a technical exercise, rather than as being linked to ethical perspectives on humanitarianism and its principles, may thus inadvertently contribute to reduced acceptability of, and ultimately reduced access for, humanitarian actors. Dunantist actors wishing to stay true to their ethical approach need new ways of thinking about accountability, a reflection which can serve as an example for an ongoing need to consider differences between actors within the humanitarian–development nexus.
Turbulence, Sediment‐Induced Stratification, and Mixing Under Macrotidal Estuarine Conditions (Qiantang Estuary, China)
Authors: Tu Junbiao , Fan Daidu , Zhang Yue , Voulgaris George 
Published: May 2019

AbstractTime series of in situ measured velocity and suspended sediment concentration from Qiantang Estuary (China), and estimates of turbulence and sediment stratification parameters are presented. The data span a period of 9 days, and after phase averaged, they are used to explore spring‐neap tidal variations in flow, turbulence, and sediment stratification. A local balance between shear production, sediment‐induced buoyancy flux, and dissipation is found to hold during ebb for both neap and spring tides. During flood elevated turbulence dissipation rates are observed, attributed to nonlocal turbulence, most likely due to horizontal advection. Our results show that the effect of sediment stratification is successfully parameterized by adding the Monin‐Obukhov length scale to the classical logarithmic layer theory. Flood‐ebb asymmetry in both Rig and Rf is observed, with higher values attained during flood due to the higher sediment concentrations and the corresponding weaker velocity shear found at these times. Rf is found to increase with Rig when Rig < 0.25, and it attains a maximum value of ~0.2. Our estimates, consistently with those from previous studies, fall slightly below the commonly used theoretical predictions. Sediment stratification contributes to the decay of turbulence, and weak mixing still exists under high Rig numbers.
Structural insights into DNA recognition by AimR of the arbitrium communication system in the SPbeta phage
Authors: Guan Zeyuan , Pei Kai , Wang Jing , Cui Yongqing , Zhu Xiang , Su Xiang , Zhou Yuanbao , Zhang Delin , Tang Chun , Yin Ping , Liu Zhu , Zou Tingting 
Published: May 2019

AbstractA newly identified arbitrium communication system regulates the lysis-to-lysogeny decision in a Bacillus bacteriophage. This system contains an arbitrium hexapeptide as a signal, the cellular receptor AimR, and the lysogenic negative regulator AimX. AimR specifically targets the downstream DNA to activate aimX gene expression. The arbitrium peptide binds to AimR, inhibiting its DNA-binding to promote phage lysogeny. Recently, we and other groups have elucidated how arbitrium peptide sensed by AimR. However, the molecular mechanisms of DNA recognition by AimR and the regulation of its DNA-binding activity by the peptide remain largely unknown. Here, we report the crystal structure of the AimR–DNA complex at 2.1 Å resolution. The N-terminal HTH motif recognizes the palindromic DNA sequence, buttressed by interactions between positively charged residues and the DNA phosphate groups. The DNA-bound AimR assembles a more closed dimer than the peptide-bound form. Single-molecule FRET and crosslinking assays revealed that the AimR protein samples both open and closed conformations in solution. Arbitrium peptide binding induces a closed-to-open conformational change of AimR, eliminating DNA targeting. Our structural and functional analysis provides new insights into the DNA recognition mechanism of AimR and its regulation by the arbitrium peptide in the context of phage lysis-lysogeny decisions.
Modulation of antibiotic sensitivity and biofilm formation in Pseudomonas aeruginosa by interspecies signal analogues
Authors: An Shi-qi , Murtagh Julie , Twomey Kate B. , Gupta Manoj K. , O’Sullivan Timothy P. , Ingram Rebecca , Valvano Miguel A. , Tang Ji-liang 
Published: May 2019

AbstractPseudomonas aeruginosa, a significant opportunistic pathogen, can participate in inter-species communication through signaling by cis-2-unsaturated fatty acids of the diffusible signal factor (DSF) family. Sensing these signals leads to altered biofilm formation and increased tolerance to various antibiotics, and requires the histidine kinase PA1396. Here, we show that the membrane-associated sensory input domain of PA1396 has five transmembrane helices, two of which are required for DSF sensing. DSF binding is associated with enhanced auto-phosphorylation of PA1396 incorporated into liposomes. Further, we examined the ability of synthetic DSF analogues to modulate or inhibit PA1396 activity. Several of these analogues block the ability of DSF to trigger auto-phosphorylation and gene expression, whereas others act as inverse agonists reducing biofilm formation and antibiotic tolerance, both in vitro and in murine infection models. These analogues may thus represent lead compounds to develop novel adjuvants improving the efficacy of existing antibiotics.
Upconverted electroluminescence via Auger scattering of interlayer excitons in van der Waals heterostructures
Authors: Binder J. , Howarth J. , Withers F. , Molas M. R. , Taniguchi T. , Watanabe K. , Faugeras C. , Wysmolek A. , Danovich M. , Fal’ko V. I. , Geim A. K. , Novoselov K. S. , Potemski M. , Kozikov A. 
Published: May 2019

AbstractThe intriguing physics of carrier-carrier interactions, which likewise affect the operation of light emitting devices, stimulate the research on semiconductor structures at high densities of excited carriers, a limit reachable at large pumping rates or in systems with long-lived electron-hole pairs. By electrically injecting carriers into WSe2/MoS2 type-II heterostructures which are indirect in real and k-space, we establish a large population of typical optically silent interlayer excitons. Here, we reveal their emission spectra and show that the emission energy is tunable by an applied electric field. When the population is further increased by suppressing the radiative recombination rate with the introduction of an hBN spacer between WSe2 and MoS2, Auger-type and exciton-exciton annihilation processes become important. These processes are traced by the observation of an up-converted emission demonstrating that excitons gaining energy in non-radiative Auger processes can be recovered and recombine radiatively.
Optimal integration of intraneural somatosensory feedback with visual information: a single-case study
Authors: Risso G. , Valle G. , Iberite F. , Strauss I. , Stieglitz T. , Controzzi M. , Clemente F. , Granata G. , Rossini P. M. , Micera S. , Baud-Bovy G. 
Published: May 2019

AbstractProviding somatosensory feedback to amputees is a long-standing objective in prosthesis research. Recently, implantable neural interfaces have yielded promising results in this direction. There is now considerable evidence that the nervous system integrates redundant signals optimally, weighting each signal according to its reliability. One question of interest is whether artificial sensory feedback is combined with other sensory information in a natural manner. In this single-case study, we show that an amputee with a bidirectional prosthesis integrated artificial somatosensory feedback and blurred visual information in a statistically optimal fashion when estimating the size of a hand-held object. The patient controlled the opening and closing of the prosthetic hand through surface electromyography, and received intraneural stimulation proportional to the object’s size in the ulnar nerve when closing the robotic hand on the object. The intraneural stimulation elicited a vibration sensation in the phantom hand that substituted the missing haptic feedback. This result indicates that sensory substitution based on intraneural feedback can be integrated with visual feedback and make way for a promising method to investigate multimodal integration processes.
Probe dependency in the determination of ligand binding kinetics at a prototypical G protein-coupled receptor
Authors: Bosma Reggie , Stoddart Leigh A. , Georgi Victoria , Bouzo-Lorenzo Monica , Bushby Nick , Inkoom Loretta , Waring Michael J. , Briddon Stephen J. , Vischer Henry F. , Sheppard Robert J. , Fernández-Montalván Amaury , Hill Stephen J. , Leurs Rob 
Published: May 2019

AbstractDrug-target binding kinetics are suggested to be important parameters for the prediction of in vivo drug-efficacy. For G protein-coupled receptors (GPCRs), the binding kinetics of ligands are typically determined using association binding experiments in competition with radiolabelled probes, followed by analysis with the widely used competitive binding kinetics theory developed by Motulsky and Mahan. Despite this, the influence of the radioligand binding kinetics on the kinetic parameters derived for the ligands tested is often overlooked. To address this, binding rate constants for a series of histamine H1 receptor (H1R) antagonists were determined using radioligands with either slow (low koff) or fast (high koff) dissociation characteristics. A correlation was observed between the probe-specific datasets for the kinetic binding affinities, association rate constants and dissociation rate constants. However, the magnitude and accuracy of the binding rate constant-values was highly dependent on the used radioligand probe. Further analysis using recently developed fluorescent binding methods corroborates the finding that the Motulsky-Mahan methodology is limited by the employed assay conditions. The presented data suggest that kinetic parameters of GPCR ligands depend largely on the characteristics of the probe used and results should therefore be viewed within the experimental context and limitations of the applied methodology.
Association between APOE4 and biomarkers in cerebral adrenoleukodystrophy
Authors: Orchard Paul J. , Markowski Todd W. , Higgins LeeAnn , Raymond Gerald V. , Nascene David R. , Miller Weston P. , Pierpont Elizabeth I. , Lund Troy C. 
Published: May 2019

AbstractCerebral adrenoleukodystrophy (cALD) is an inflammatory neurodegenerative disease associated with mutation of the ABCD1 gene. Proteomic analysis of cerebral spinal fluid (CSF) from young males with active cALD revealed markers of inflammation including APOE4. APOE4 genotype has been associated with an inferior prognosis following acute and chronic neurologic injury. We assessed APOE4 inheritance among 83 consecutive young males with cALD prior to hematopoietic cell transplant and its association with markers of cerebral disease. The allele frequency of APOE4 was not significantly different from that of the general population at 17%. Young males with cALD that were APOE4 carriers had similar CSF protein and chitotriosidase activity to that of non-carriers. In contrast, APOE4 carriers had an increased burden of cerebral disease involvement as determined by MRI severity score (10.5 vs 7.0 points, p = 0.01), higher gadolinium intensity score (2.0 vs 1.3 points, p = 0.007), inferior neurologic function (neurologic function score 2.4 vs 1.0, p = 0.001), and elevated CSF MMP2 levels compared to that of non-carriers (13168 vs 9472 pg/mL, p = 0.01). These are the first data showing that APOE4 is associated with increased severity of cerebral disease in cALD and suggest it may be a modifier of disease.
Voluntary upregulation of heart rate variability through biofeedback is improved by mental contemplative training
Authors: Bornemann Boris , Kovacs Peter , Singer Tania 
Published: May 2019

AbstractRegulation of the parasympathetic nervous system, indexed through high frequency heart rate variability (HF-HRV), is indicative of physical and psychological health. However, little is known about the trainability of this capacity. We investigated the effects of a 9-month mental training program (the ReSource Project; n = 298) on voluntary HF-HRV upregulation, assessed with a novel biofeedback procedure. The program consisted of attentional, interoceptive, socio-affective and socio-cognitive training elements, all of which potentially influence parasympathetic regulation. Based on known links between oxytocin and parasympathetic activity, we also explored the relationship of HF-HRV upregulation to the oxytocin receptor system. We found that HF-HRV during the biofeedback session increased after 3 months of training, concomitant with prolonged respiration cycles. Breathing-controlled changes in HF-HRV upregulation, indicative of improved parasympathetic control, were significantly increased after 6 months of training. Homozygous risk allele carriers (AA) of the oxytocin receptor gene polymorphism rs53576 showed initially lower parasympathetic control, but fully compensated for their initial deficits through the training. No changes were found for HF-HRV at rest. Our data demonstrate that a mental training intervention extending over several months can increase the capacity for voluntary regulation of HF-HRV, with important implications for improving individual and societal health.
Co-expression network analysis identifies gonad- and embryo-associated protein modules in the sentinel species Gammarus fossarum
Authors: Degli Esposti Davide , Almunia Christine , Guery Marc-Antoine , Koenig Natacha , Armengaud Jean , Chaumot Arnaud , Geffard Olivier 
Published: May 2019

AbstractNext generation sequencing and mass spectrometry technologies have recently expanded the availability of whole transcriptomes and proteomes beyond classical model organisms in molecular biology, even in absence of an annotated genome. However, the fragmented nature of transcriptomic and proteomic data reduces the ability to interpret the data, notably in non-model organisms. Network-based approaches may help extracting important biological information from -omics datasets. The reproductive cycle of the freshwater crustacean Gammarus fossarum.provides an excellent case study to test the relevance of a network analysis in non-model organisms. Here, we illustrated how the use of a co-expression network analysis (based on Weighted Gene Co-expression Network Analysis algorithm, WGCNA) allowed identifying protein modules whose expression profiles described germ cell maturation and embryonic development in the freshwater crustacean Gammarus fossarum. Proteome datasets included testes, ovaries or embryos samples at different maturation or developmental stages, respectively. We identified an embryonic module correlated with mid-developmental stages corresponding to the organogenesis and it was characterized by enrichment in proteins involved in RNA editing and splicing. An ovarian module was enriched in vitellogenin-like proteins and clottable proteins, confirming the diversity of proteins belonging to the large lipid transfer family involved in oocytes maturations in this freshwater amphipod. Moreover, our results found evidence of a fine-tuned regulation between energy production by glycolysis and actin-myosin-dependent events in G. fossarum spermatogenesis. This study illustrates the importance of applying systems biology approaches to emergent animal models to improve the understanding of the molecular mechanisms regulating important physiological events with ecological relevance.
Thermoelectric properties enhancement of p-type composite films using wood-based binder and mechanical pressing
Authors: Jang Eunhwa , Poosapati Aswani , Jang Nathaniel , Hu Liangbing , Duffy Michael , Zupan Marc , Madan Deepa 
Published: May 2019

AbstractThermoelectric generators (TEGs) fabricated using additive manufacturing methods are attractive because they offer the advantages of scalability, lower cost, and potentially higher power density than conventional TEGs. Additive manufacturing of TEGs requires active thermoelectric particles to be dispersed in a polymer binder to synthesize printable slurries, and printed films to be subsequently subjected to a long and high temperature curing to enhance their thermoelectic properties. A large amount of polymer binder present in composite films results in a sizable loss in the electrical conductivity. In addition, a long and high-temperature film curing results is a slow and energy intensive fabrication process. In this work, we demonstrate the feasibility of using a small amount (≤10−3 wt ratio) of novel nanofiber cellulose (NFC) as a binder to provide sufficient adhesion strength to hold the TE particles together in the composite films. We also demonstrate a pressure induced densification process to enhance the thermoelectic properties of printed composite films. This novel approach has the potential to fundamentally transform the manufacting method for printing TEGs by eliminating the need of long-duration and high-temperature curing. A higher applied pressure leads to a compact packing and densification of films resulting in an improvement in the electrical conductivity. The highest power factor achieved for best performing p-type thermoelectric-NFC composite film subjected to pressure induced densification is 611 μW/m-K2.
The transcriptome of Cryptosporidium oocysts and intracellular stages
Authors: Matos Lucas V. S. , McEvoy John , Tzipori Saul , Bresciani Katia D. S. , Widmer Giovanni 
Published: May 2019

AbstractHuman cryptosporidiosis is caused primarily by two species of apicomplexan parasites, Cryptosporidium parvum and C. hominis. Although infection of cell monolayers with sporozoites does not support the complete parasite life cycle, the in vitro system is used to study the asexual phase of multiplication, which consists of two generations of merogony. To better understand host-parasite interaction and to gain insight into gene regulatory processes driving the complex life cycle of Cryptosporidium parasites, we analyzed the transcriptome of C. parvum in oocysts, sporozoites and infected cell monolayers 2–48 h post-infection. Analysis of RNA-Seq data from replicate oocyst, sporozoite and intracellular samples revealed significant differences between transcriptomes expressed outside and inside the host cell. Compared to the transcriptome found in the host cell, the oocyst transcriptome is less diverse. Biological processes significantly over-represented intracellularly relate to biosynthetic processes. Genes significantly overexpressed in oocysts show evidence of specialized functions not found in other Apicomplexa. A more comprehensive view of gene regulation during the Cryptosporidium life cycle will require the analysis of later time points during the infection, particularly of the poorly studied sexual phase of the life cycle.
Quantitation of the neural silencing activity of anion channelrhodopsins in Caenorhabditis elegans and their applicability for long-term illumination
Authors: Yamanashi Taro , Maki Misayo , Kojima Keiichi , Shibukawa Atsushi , Tsukamoto Takashi , Chowdhury Srikanta , Yamanaka Akihiro , Takagi Shin , Sudo Yuki 
Published: May 2019

AbstractIon pumps and channels are responsible for a wide variety of biological functions. Ion pumps transport only one ion during each stimulus-dependent reaction cycle, whereas ion channels conduct a large number of ions during each cycle. Ion pumping rhodopsins such as archaerhodopsin-3 (Arch) are often utilized as light-dependent neural silencers in animals, but they require a high-density light illumination of around 1 mW/mm2. Recently, anion channelrhodopsins -1 and -2 (GtACR1 and GtACR2) were discovered as light-gated anion channels from the cryptophyte algae Guillardia theta. GtACRs are therefore expected to silence neural activity much more efficiently than Arch. In this study, we successfully expressed GtACRs in neurons of the nematode Caenorhabditis elegans (C. elegans) and quantitatively evaluated how potently GtACRs can silence neurons in freely moving C. elegans. The results showed that the light intensity required for GtACRs to cause locomotion paralysis was around 1 µW/mm2, which is three orders of magnitude smaller than the light intensity required for Arch. As attractive features, GtACRs are less harmfulness to worms and allow stable neural silencing effects under long-term illumination. Our findings thus demonstrate that GtACRs possess a hypersensitive neural silencing activity in C. elegans and are promising tools for long-term neural silencing.
Post-tilleyite, a dense calcium silicate-carbonate phase
Authors: Santamaria-Perez David , Ruiz-Fuertes Javier , Peña-Alvarez Miriam , Chulia-Jordan Raquel , Marqueño Tomas , Zimmer Dominik , Gutiérrez-Cano Vanessa , MacLeod Simon , Gregoryanz Eugene , Popescu Catalin , Rodríguez-Hernández Plácida , Muñoz Alfonso 
Published: May 2019

AbstractCalcium carbonate is a relevant constituent of the Earth’s crust that is transferred into the deep Earth through the subduction process. Its chemical interaction with calcium-rich silicates at high temperatures give rise to the formation of mixed silicate-carbonate minerals, but the structural behavior of these phases under compression is not known. Here we report the existence of a dense polymorph of Ca5(Si2O7)(CO3)2 tilleyite above 8 GPa. We have structurally characterized the two phases at high pressures and temperatures, determined their equations of state and analyzed the evolution of the polyhedral units under compression. This has been possible thanks to the agreement between our powder and single-crystal XRD experiments, Raman spectroscopy measurements and ab-initio simulations. The presence of multiple cation sites, with variable volume and coordination number (6–9) and different polyhedral compressibilities, together with the observation of significant amounts of alumina in compositions of some natural tilleyite assemblages, suggests that post-tilleyite structure has the potential to accommodate cations with different sizes and valencies.