bluuebunny/crossref_metadata_2025_split huggingface dataset:
Signaling pathways and gene co-expression modules associated with cytoskeleton and axon morphology in breast cancer survivors with chronic paclitaxel-induced peripheral neuropathy
Authors: Kober Kord M , Schumacher Mark , Conley Yvette P , Topp Kimberly , Mazor Melissa , Hammer Marilynn J , Paul Steven M , Levine Jon D , Miaskowski Christine 
Published: September 2019

Background The major dose-limiting toxicity of paclitaxel, one of the most commonly used drugs to treat breast cancer, is peripheral neuropathy (paclitaxel-induced peripheral neuropathy). Paclitaxel-induced peripheral neuropathy, which persists into survivorship, has a negative impact on patient’s mood, functional status, and quality of life. Currently, no interventions are available to treat paclitaxel-induced peripheral neuropathy. A critical barrier to the development of efficacious interventions is the lack of understanding of the mechanisms that underlie paclitaxel-induced peripheral neuropathy. While data from preclinical studies suggest that disrupting cytoskeleton- and axon morphology-related processes are a potential mechanism for paclitaxel-induced peripheral neuropathy, clinical evidence is limited. The purpose of this study in breast cancer survivors was to evaluate whether differential gene expression and co-expression patterns in these pathways are associated with paclitaxel-induced peripheral neuropathy. Methods Signaling pathways and gene co-expression modules associated with cytoskeleton and axon morphology were identified between survivors who received paclitaxel and did (n = 25) or did not (n = 25) develop paclitaxel-induced peripheral neuropathy. Results Pathway impact analysis identified four significantly perturbed cytoskeleton- and axon morphology-related signaling pathways. Weighted gene co-expression network analysis identified three co-expression modules. One module was associated with paclitaxel-induced peripheral neuropathy group membership. Functional analysis found that this module was associated with four signaling pathways and two ontology annotations related to cytoskeleton and axon morphology. Conclusions This study, which is the first to apply systems biology approaches using circulating whole blood RNA-seq data in a sample of breast cancer survivors with and without chronic paclitaxel-induced peripheral neuropathy, provides molecular evidence that cytoskeleton- and axon morphology-related mechanisms identified in preclinical models of various types of neuropathic pain including chemotherapy-induced peripheral neuropathy are found in breast cancer survivors and suggests pathways and a module of genes for validation and as potential therapeutic targets.
Effect of visual feedback on the performance of the star excursion balance test
Authors: Wan Yi , Davies Jennifer L , Button Kate , Al-Amri Mohammad 
Published: September 2019

Introduction Visual feedback is an effective method to enhance postural and balance control in clinical and sports training. The aim of this study was to explore the effect of real-time visual feedback provided by a video camera on the performance of a dynamic balance test, which is the star excursion balance test in healthy subjects. Methods We compared the performance of the star excursion balance test using the maximum reach distance in 20 healthy participants (10 male and 10 female, 26.8 ± 3.7 years) under two conditions: without feedback and whilst they viewed their movements in real-time on a screen in front of them via a video camera. Results The results showed that real-time visual feedback had a significant effect on maximum reach distance of the star excursion balance test in the posterolateral direction (P < 0.001). There was a non-significant increase in the maximum reach distance in the anterior and posteromedial directions. Conclusion The result indicates that the real-time visual feedback appears to be an effective means for improving the performance of the star excursion balance test in the posterolateral direction, and may be a promising tool for clinical rehabilitation and athlete training to enhance dynamic postural control.
The Threatful Self: Midbrain Functional Connectivity to Cortical Midline and Parietal Regions During Subliminal Trauma-Related Processing in PTSD
Authors: Terpou Braeden A. , Densmore Maria , Théberge Jean , Thome Janine , Frewen Paul , McKinnon Margaret C. , Lanius Ruth A. 
Published: September 2019

Background The innate alarm system consists of a subcortical network of interconnected midbrain, lower brainstem, and thalamic nuclei, which together mediate the detection of evolutionarily-relevant stimuli. The periaqueductal gray is a midbrain structure innervated by the innate alarm system that coordinates the expression of defensive states following threat detection. In participants with post-traumatic stress disorder, the periaqueductal gray displays overactivation during the subliminal presentation of trauma-related stimuli as well as altered resting-state functional connectivity. Aberrant functional connectivity is also reported in post-traumatic stress disorder for the default-mode network, a large-scale brain network recruited during self-referential processing and autobiographical memory. Here, research lacks investigation on the extent to which functional interactions are displayed between the midbrain and the large-scale cortical networks in post-traumatic stress disorder. Methods Using a subliminal threat presentation paradigm, we investigated psycho-physiological interactions during functional neuroimaging in participants with post-traumatic stress disorder (n = 26) and healthy control subjects (n = 20). Functional connectivity of the periaqueductal gray was investigated across the whole-brain of each participant during subliminal exposure to trauma-related and neutral word stimuli. Results As compared to controls during subliminal threat presentation, the post-traumatic stress disorder group showed significantly greater periaqueductal gray functional connectivity with regions of the default-mode network (i.e., angular gyrus, precuneus, superior frontal gyrus). Moreover, multiple regression analyses revealed that the functional connectivity between the periaqueductal gray and the regions of the default-mode network correlated positively to symptoms of avoidance and state dissociation in post-traumatic stress disorder. Conclusion Given that the periaqueductal gray engages the expression of defensive states, stronger midbrain functional coupling with the default-mode network may have clinical implications to self-referential and trauma-related processing in participants with post-traumatic stress disorder.
Clinical Molecular Marker Testing Data Capture to Promote Precision Medicine Research Within the Cancer Research Network
Authors: Burnett-Hartman Andrea N. , Udaltsova Natalia , Kushi Lawrence H. , Neslund-Dudas Christine , Rahm Alanna Kulchak , Pawloski Pamala A. , Corley Douglas A. , Knerr Sarah , Feigelson Heather Spencer , Hunter Jessica Ezzell , Tabano David C. , Epstein Mara M. , Honda Stacey A. , Ter-Minassian Monica , Lynch Julie A. , Lu Christine Y. 
Published: September 2019

PURPOSE To evaluate health care systems for the availability of population-level data on the frequency of use and results of clinical molecular marker tests to inform precision cancer care. METHODS We assessed cancer-related molecular marker test data availability across 12 US health care systems in the Cancer Research Network. Overall, these systems provide care to a diverse population of more than 12 million people in the United States. We performed qualitative analyses of test data availability for five blood-based protein, nine germline, and 14 tissue-based tumor marker tests in each health care system’s electronic health record and tumor registry using key informants, test code lists, and manual review of data types and output. We then performed quantitative analyses to estimate the proportion of patients with cancer with test utilization data and results for specific molecular marker tests. RESULTS Health systems were able to systematically capture population-level data on all five blood protein markers, six of 14 tissue-based tumor markers, and none of the nine germline markers. Successful, systematic data capture was achievable for tests with electronic data feeds for test results (blood protein markers) or through prior manual abstraction by tumor registrars (select tumor-based markers). For test results stored in scanned image files (particularly germline and tumor marker tests), information on which test was performed and test results was not readily accessible in an electronic format. CONCLUSION Even in health care systems with sophisticated electronic health records, there were few codified data elements available for evaluating precision cancer medicine test use and results at the population level. Health care organizations should establish standards for electronic reporting of precision medicine tests to expedite cancer research and facilitate the implementation of precision medicine approaches.
A Family With a Carotid Body Paraganglioma and Thyroid Neoplasias With a New SDHAF2 Germline Variant
Authors: Wolf Katherine I , Jacobs Michelle F , Mehra Rohit , Begani Priya , Davenport Matthew S , Marentette Lawrence J , Basura Gregory J , Hughes David T , Else Tobias 
Published: September 2019

Abstract
At least 30% of all pheochromocytomas (PCCs)/paragangliomas (PGLs) arise in patients with a germline predisposition syndrome. Variants in succinate dehydrogenase subunits A, B, C, and D (SDHA, SDHB, SDHC, and SDHD) are the most common pathogenic germline alterations. Few pathogenic variants have been reported in succinate dehydrogenase assembly factor 2 (SDHAF2). Here, we describe a 30-year-old female patient who presented with a left-sided neck mass, which was later characterized as a carotid body PGL. Genetic testing revealed a likely pathogenic SDHAF2 variant (c.347G>A;p.W116X). Two sisters carried the same pathologic variant, and screening protocols were recommended. Whole-body MRI revealed thyroid nodules; this testing was followed by fine-needle aspiration, which confirmed papillary thyroid carcinoma in one sister and a follicular adenoma in the other. The two sisters then underwent hemithyroidectomy and total thyroidectomy, respectively. Because evidence for pathogenic variants in SDHAF2 causing predisposition to PCC/PGL is limited, we discuss the challenges in mutational variant interpretation and decision making regarding screening for associated tumors.
Calcifediol Decreases Interleukin-6 Secretion by Cultured Human Trophoblasts From GDM Pregnancies
Authors: Lacroix Marilyn , Lizotte Farah , Hivert Marie-France , Geraldes Pedro , Perron Patrice 
Published: September 2019

Abstract
Gestational diabetes mellitus (GDM) is often characterized by low maternal calcifediol (25OHD) and high inflammation levels. This study aimed to determine whether placental protein expressions of CYP27B1, vitamin D receptor (VDR), and CYP24A1 are impaired in GDM and to investigate the effect of a 25OHD treatment on IL-6 secretion by GDM trophoblasts compared with normoglycemic (NG) trophoblasts. Placental tissue samples were harvested to determine protein expression of CYP27B1, VDR, and CYP24A1 by immunoblots. Isolated trophoblasts were stimulated with 25OHD concentrations (25 to 2000 nM) once a day for 3 days and IL-6 secretion was quantified (ELISA). We recruited 17 NG women, 19 women with GDM treated with diet and exercise alone (GDM-d) and 9 women with GDM who necessitated insulin therapy (GDM-i). Protein expressions of CYP27B1 and VDR were significantly higher in placental tissue from GDM-d women compared with NG women (both P = 0.02), whereas no differences were detected between GDM-i and NG placental tissues. In cultured trophoblasts (two groups; n = 5 NG and n = 5 GDM-d), exposure to increasing 25OHD concentrations significantly decreased IL-6 secretion in the GDM-d group only (P = 0.006). After treatment with 25OHD (2000 nM), IL-6 secretion was lower in the GDM-d group compared with the NG group (P = 0.03). Our results suggest an upregulation of the VDR-1,25(OH)2D complex bioavailability in GDM-d placentas, possibly reflecting a compensatory mechanism aiming to ensure that vitamin D can exert its genomic and nongenomic effects in the target cells of the placental-fetal unit. Our findings support an anti-inflammatory effect of vitamin D at the feto-maternal interface in GDM-d pregnancies.
E-Cigarette Exposure Delays Implantation and Causes Reduced Weight Gain in Female Offspring Exposed In Utero
Authors: Wetendorf Margeaux , Randall Lewis T , Lemma Mahlet T , Hurr Sophia H , Pawlak John B , Tarran Robert , Doerschuk Claire M , Caron Kathleen M 
Published: September 2019

Abstract
Electronic nicotine delivery system (e-cigarette) use is prevalent among pregnant women as a seemingly safe alternative to traditional tobacco use, known to result in fetal developmental abnormalities and impaired fertility of male offspring. However, little is known about the effects of e-cigarette use on fertility or pregnancy outcomes. A successful pregnancy is initiated by a multitude of dynamic molecular alterations in the uterus resulting in embryo implantation at day 4.5 in the mouse. We examined whether e-cigarette exposure impairs implantation and offspring health. Pregnant C57BL/6J mice were exposed five times a week to e-cigarette vapor or sham. After 4 months, e-cigarette exposed dams exhibited a significant delay in the onset of the first litter. Furthermore, exposure of new dams in early pregnancy significantly impaired embryo implantation, as evidenced by nearly complete absence of implantation sites in e-cigarette–exposed animals at day 5.5, despite exhibiting high levels of progesterone, an indicator of pregnancy. RNA microarray from day 4.5 pseudopregnant mice revealed significant changes in the integrin, chemokine, and JAK signaling pathways. Moreover, female offspring exposed to e-cigarettes in utero exhibited a significant weight reduction at 8.5 months, whereas males exhibited a slight but nonsignificant deficiency in fertility. Thus, e-cigarette exposure in mice impairs pregnancy initiation and fetal health, suggesting that e-cigarette use by reproductive-aged women or during pregnancy should be considered with caution.
Cold Crystallization of Chiral Schiff-Base Nickel(II) Complex Having Alkyl Chains and a Characteristic Methyl Group
Authors: Honda Akinori , Yoshida Takashi , Shioda Ayumi , Nomoto Kuniharu , Miyamura Kazuo 
Published: September 2019

Abstract
Molecules exhibiting cold crystallization, an exothermic phenomenon in heating following supercooling, can be used as a heat storing material. On the other hand, examples in non-polymers are still few. A chiral Schiff-base nickel(II) complex, which had a characteristic methyl group and long alkyl chains, exhibited complicated thermal behavior including cold crystallization. The methyl group was a steric barrier to dimerization and molecular stacking, leading to the supercooled state. In addition, the thermal behavior of enantiomer was different from that of racemate.
Isolation and initial propagation of guinea pig adenovirus (GPAdV) in Cavia porcellus cell lines
Authors: Kajon Adriana E. , Li Xiaoxin , Gonzalez Gabriel , Core Susan , Hofmann-Sieber Helga , Leng Shuguang 
Published: September 2019

Background: The lack of adequate in vitro systems to isolate and propagate guinea pig adenovirus (GPAdV), a prevalent cause of respiratory illness of varaible severity in laboratory guinea pig colonies worldwide, has precluded its formal characterization to allow for the development of comprehensive diagnostic assays, and for the execution of complex pathogenesis and basic virology studies. Methods: Two strains of GPAdV were isolated in guinea pig (Cavia porcellus) cell cultures from frozen archival infected animal tissue originated from colony outbreaks of pneumonia in Australia and the Czech Republic in 1996. Results: Commercially available guinea pig cell lines from colorectal carcinoma (GPC-16), fetal fibroblast (104-C1) and lung fibroblast (JH4 C1), and the tracheal epithelial cell line GPTEC-T developed in this study were able to support viral infection and early propagation. Sufficient viral DNA was recovered from cell cultures to PCR-amplify and obtain sequence data for the complete hexon gene and partial DNA polymerase and penton base genes. Phylogenetic analysis for the three regions of the genome provided strong evidence confirming GPAdV as a unique species in the genus Mastadenovirus. Conclusions: This study demonstrated the feasibility of propagating GPAdV in cultures of immortalized lines of GP cells of a variety of types, thus establishing a critical foundation for the development of a robust culture platform for virus stock production and titration. The generation and analysis of whole GPAdV genome sequences will provide additional data for a comprehensive description of the genetic organization of the viral genome and for a better assessment of genetic diversity between the two isolated strains.
REFLEXÕES SOBRE A PROVA CIENTÍFICA NO PROCESSO CIVIL BRASILEIRO
Authors: De Luna Rafael Alves , Gomes Neto José Mário Wanderley , De Oliveira Gabriela Suele Carneiro 
Published: September 2019

Ante a presença dessas provocações doutrinárias, os pensamentos se direcionam a questionar: como as provas reputadas como científicas devem ser lidas e interpretadas pelo magistrado? Buscando respostas no Código de Processo Civil de 2015, viu-se que a proposta de admissibilidade – se é que há uma propriamente dita – mostra-se estanque e refratária de toda a influência científico-tecnológica no nosso contexto social, sendo inconcebível tal fato. A fronteira entre ciência e direito não se encontra demarcada, o que traz riscos tanto a uma quanto a outra. Advertindo previamente o leitor, a interação entre direito e ciência não é nenhuma novidade. O Código de Processo Civil de 1939 continha um capítulo destinado ao tema dos “exames periciais”. Entretanto, é inegável que a ciência/tecnologia é bem mais presente em nossas vidas e o acesso a ela é bem mais fácil do que no século passado, o que sem dúvida aumenta o nível de utilização dessa espécie probatória.
REFLEXÕES SOBRE A PROPOSTA DE FORMAÇÃO DE PROFESSORES DO PNAIC PARA A EDUCAÇÃO INFANTIL
Authors: França da Silva Cármen Maria , Ortiz de Leão Débora 
Published: September 2019

Resumo: Entre 2017/2018, o Ministério da Educação incluiu a etapa, Educação Infantil, no Programa Pacto Nacional pela Alfabetização na Idade Certa – PNAIC –, conduzindo a reflexão sobre temáticas referentes às políticas curriculares e formação de professores que atuam nesse contexto. O objetivo deste estudo bibliográfico é discutir a implementação desse programa na Educação Infantil. Inicialmente, realizamos um resgate histórico sobre o PNAIC. Posteriormente, apresentamos a concepção de criança e infância, a partir dos conceitos que se constituíram ao longo da história no Brasil. Após, apresentamos uma análise dos marcos que sustentam a ação pedagógica na Educação Infantil. Por fim, realizamos uma análise da proposta construída, na tentativa de elucidar a sua repercussão nas práticas educativas.Palavras-chave: Pacto Nacional pela Alfabetização na Idade Certa. Educação Infantil. Alfabetização.
APONTAMENTOS SOBRE O EDUCADOR AUDIOVISUAL: CINEMA, EDUCAÇÃO E ALTERIDADE
Authors: Guerreiro Alexandre Silva 
Published: September 2019

O presente artigo busca refletir sobre o educador audiovisual como sujeito intrínseco à relação que se estabelece entre cinema e educação, na medida em que a essa relação atrelamos o conceito de alteridade. Para isso, implementamos uma investigação sobre a escola e o papel do educador para, em seguida, explorarmos os conceitos de teórico amador, criado por Jacques Rancière (2012) como um delineador do educador na contemporaneidade, e de alteridade, conforme formulada por Emanuel Lévinas (1988), entendendo o Outro como central. Essas duas abordagens nos conduzem à formulação de que os atores sociais envolvidos nessa relação são sujeitos de afeto, atravessados pelas questões que envolvem cinema, educação e alteridade na contemporaneidade.
Open-Access-Verträge in der Schweiz – ein Werkstattbericht aus dem Konsortium der Schweizer Hochschulbibliotheken
Authors: Boutsiouci Pascalia , Felder Fabian 
Published: September 2019

Zusammenfassung
Open-Access hat sich in der Schweiz in den letzten Jahren von einer dezentralen in eine nationale Bewegung gewandelt. Die Open-Access-Strategie von swissuniversities unterstützt dabei den einheitlichen Ansatz, alle mit öffentlichen Geldern finanzierten wissenschaftlichen Publikationen bis zum Jahr 2024 im Internet frei zugänglich zu machen. Die Neuorganisation der Verhandlungen mit Elsevier, Springer Nature und Wiley sowie weitere Aktivitäten des Konsortiums der Schweizer Hochschulbibliotheken bilden eine erste Grundlage, um die globale Transformation zu Open Access auch aus der Schweiz zu unterstützen und zu fördern.
DIMAG – Programmpaket zur digitalen Langzeitarchivierung im Verbund
Authors: Laux Susanne 
Published: September 2019

Zusammenfassung
Digitale Langzeitarchivierung wird von dem einschlägigen Standard OAIS als „Erhalt digital gespeicherter Informationen über die Lebensdauer der heutigen Hard- und Software hinaus“ definiert. Der DIMAG-Verbund hat es sich zur Aufgabe gemacht, hierfür unter anderem auch technische Lösungen zu entwickeln. Mit der Software DIMAG wächst ein großes Programmpaket, das für alle Aufgaben der digitalen Langzeitarchivierung – von der Vorbereitung der Übernahme digitaler Daten bis hin zur Migration – einsetzbar ist.
Broad-Band Substrate-Free Planar Metamaterial Lens Based on a Geometric Transformation of Polygon
Authors: Pen Tianyu , Huang Bing , Sun Hao , Sun Xiaowei , Li Lingyun 
Published: September 2019

Abstract
A broad-band substrate-free planar metamaterial lens fed by a horn antenna is proposed. A geometric transformation of polygon is introduced in order to make gradient tuneable unit cells out of ordinary metamaterial structures. A circular lens with gradient epsilon along its surface is designed and fabricated based on the transform theory. Measurement results indicate that the proposed lens is capable of improving the gain of the horn antenna within the frequency band of 78–89 GHz which is more than doubled as comparing with existing substrate-free metamaterial lenses.
A Simple but Efficient Catalytic Approach for the Degradation of Pollutants in Aqueous Media through Cicer arietinum Supported Ni Nanoparticles
Authors: Khan Shahid Ali , Rasool Shagufta , Rahman Khaliq Ur , Hussain Shah , Khan Inamullah , Ismail Muhammad , Farooq Aliya , Khan Sarzamin , Raza Mian Ahmad , Asiri Abdullah Muhammad , Khan Sher Bahadar 
Published: September 2019

Abstract
Plant based materials are considered to have broad applications in the remediation of toxic materials. In this report, we present an environmental friendly and economic Cicer arietinum, named as (CP) supported for the synthesis of Ni nanoparticles (NPs) designated as Ni@CP. The in situ Ni@CP NPs were obtained using aqueous medium in the presence of sodium borohydride (NaBH4) as a reducing agent. The prepared catalysts were applied for the hydrogenation/degradation of p-nitrophenol (PNP), o-nitrophenol (ONP), and 2,4-dinitrophenol (DNP), as well as congo red (CR), methyl orange (MO), methylene blue (MB) and rhodamine B (RB) dyes. The amount of total metal ions adsorbed onto the CP was evaluated by flame atomic absorption spectroscopy. The Ni@CP catalyst was characterized through PXRD, FTIR, FESEM and EDX analyses.