bluuebunny/crossref_metadata_2025_split huggingface dataset:
Stirred Suspension Bioreactor Culture of Porcine Induced Pluripotent Stem Cells
Authors: Burrell Kyle , Dardari Rkia , Goldsmith Taylor , Toms Derek , Villagomez Daniel A.F. , King W. Allan , Ungrin Mark , West Franklin D. , Dobrinski Ina 
Published: May 2019

AbstractInduced pluripotent stem cells (iPSCs) are an attractive cell source for regenerative medicine and the development of therapies, as they can proliferate indefinitely under defined conditions and differentiate into any cell type in the body. Large scale expansion of cells is limited in adherent culture, making it difficult to obtain adequate cell numbers for research. It has been previously shown that stirred suspension bioreactors (SSBs) can be used to culture mouse and human stem cells. Pigs are important pre-clinical models for stem cell research. Therefore, this study investigated the use of SSBs as an alternative culture method for the expansion of iPSCs. Using an established porcine iPSC line as well as a new cell line derived and characterized in the current study, we report that porcine iPSCs (piPSCs) can grow in SSB while maintaining characteristics of pluripotency and karyotypic stability similar to cells grown in traditional two-dimensional static culture. This culture method provides a suitable platform for scale up of cell culture to provide adequate cell numbers for future research applications involving porcine induced pluripotent stem cells.
Left parietal tACS at alpha frequency induces a shift of visuospatial attention
Authors: Schuhmann T. , Kemmerer S. K. , Duecker F. , de Graaf T.A. , ten Oever S. , De Weerd P. , Sack A.T. 
Published: May 2019

AbstractBackgroundVoluntary shifts of visuospatial attention are associated with a lateralization of occipitoparietal alpha power (7-13Hz), i.e. higher power in the hemisphere ipsilateral and lower power contralateral to the locus of attention. Recent noninvasive neuromodulation studies demonstrated that alpha power can be experimentally increased using transcranial alternating current stimulation (tACS).Objective/HypothesisWe hypothesized that tACS at alpha frequency over the left parietal cortex induces shifts of attention to the left hemifield. However, spatial attention shifts not only occur voluntarily (endogenous), but also stimulus-driven (exogenous). In order to study the task-specificity of the potential effects of tACS on attentional processes, we administered three conceptually different spatial attention tasks.Methods36 healthy volunteers were recruited from an academic environment. In two seperate sessions, we applied either high-density tACS at 10Hz, or sham tACS, for 35-40 minutes to their left parietal cortex. We systematically compared performance on endogenous attention, exogenous attention, and stimulus detection tasks.ResultsIn the Endogenous attention task, we found a greater leftward bias in reaction times during left parietal 10Hz tACS as compared to sham. There were no stimulation effects in the exogenous attention or stimulus detection task.ConclusionThe study shows that high-density tACS at 10Hz can be used to modulate visuospatial attention performance. The tACS effect is task-specific, indicating that not all forms of attention are equally susceptible to the stimulation.
Advances in intellectual capital performance measurement: a state-of-the-art review
Authors: Lee Cheng Sheng , Wong Kuan Yew 
Published: May 2019

Purpose
The purpose of this paper is to present a review on the latest developments, approaches and methods in intellectual capital (IC) performance measurement.

Design/methodology/approach
A total of 53 research papers from high-ranking journals concerning the advances in IC performance measurement were thoroughly reviewed and classified to identify the advanced approaches and methods that have been used to evaluate IC performance.

Findings
It is clear that the current application of advanced or intelligent methods in IC performance measurement is still at the primary stage. The reviewed approaches mainly focus on two core applications. First is to assist the data collection or data handling process and second is to enhance the result computation process to obtain a more reliable and realistic value that represents the performance of IC.

Research limitations/implications
This study offers a foundation and guideline for the researchers who wish to integrate advanced methods into IC performance measurement based on the reviewed advanced approaches in terms of their respective functions, strengths and limitations. It stimulates the creation of new IC performance measurement models that use advanced approaches to cope with real-life problems and dynamic conditions.

Originality/value
This paper contributes to the literature on IC performance measurement, in light of advances in technology. In general, five advanced approaches were identified, analyzed and discussed. Future research directions were presented to provide new insights for researchers to apply advanced methods in IC performance measurement, together with theoretical and managerial implications.
State-of-the-art and adoption of artificial intelligence in retailing
Authors: Weber Felix Dominik , Schütte Reinhard 
Published: May 2019

PurposeIn the most abstract way, artificial intelligence (AI) allows human work to be shifted toward technological systems that are currently not fully capable. Following this, the domain of retail can be sketched as a natural fit for the application of AI tools, which are known for their high proportion of human work and concurrent low profit margins. This paper aims to explore the current dissemination of the application of AI within the industry. The value-added core tasks of retail companies are examined to determine the possible utilization and the market adoption within the globally largest retail companies is given.Design/methodology/approachThe paper uses two different approaches to identify the scientific state-of-the-art: a search on the major scientific databases and an empirical study of the ten largest international retail companies and their adoption of AI technologies in the domains of wholesale and retail.FindingsThe application within the different value-added core tasks varies greatly depending on the area. In summary, there are numerous possible applications in all areas. Especially, in areas where future forecasts are needed within the task areas (such as marketing or replenishment), the use of AI, today, is both scientifically and practically highly developed. In contrast, the market adoption of AI is highly variable. The pioneers have integrated extensive applications into everyday business, while the challengers are investing heavily in new initiatives. Some others, however, show neither active use nor any effort to adopt such technology.Originality/valueTo the best of the author’s knowledge, this is one of the first research contributions to analyze the areas of application and the impact of AI structured along the value-added core processes of retail companies.
An assessment of education, in-service training and tenure prolongation as methods for enhancing nursing performance
Authors: Asiamah Nestor , Mensah Henry Kofi , Ocra Ben 
Published: May 2019

Purpose
The purpose of this paper is to provide an empirical basis for considering in-service training, tenure prolongation and continuing education as methods for enhancing nursing performance.

Design/methodology/approach
A self-reported questionnaire was used to collect data from 532 nurses, who were selected using the simple random sampling method from ten hospitals in Accra North, Ghana. Confirmatory factor analysis (CFA) was used to test the study’s hypotheses.

Findings
The resulting model is of good fit at 5 per cent significance level (χ2=1.492, p=0.222), with in-service training found to be the ultimate method for enhancing nursing performance. The fitted CFA model also shows that in-service training is positively associated with education and tenure at 1 per cent significance level (p<0.001). The overall evidence suggests that training, continuing formal education and tenure prolongation are methods for improving nursing performance.

Originality/value
Apart from its contribution to the literature, this study applies validated primary data to empirically identify key methods for enhancing nursing performance.
The rainbow sign: climate change and corporate reputation
Authors: Hirsch Peter Buell 
Published: May 2019

Purpose
This paper aims to highlight the increasing importance corporate responses to climate change will play in corporate reputation.

Design/methodology/approach
Review of the relevant secondary literature

Findings
Companies that develop active responses to climate change will see positive impact on their corporate reputation.

Research limitations/implications
The review of the secondary literature is not comprehensive and subject to the author’s qualitative interpretation of it.

Practical implications
Companies that follow the recommendations will see a benefit in stakeholder appreciation.

Social implications
The more companies embrace a proactive stance on climate change, the more likely society is to meet the 2 per cent goal.

Originality/value
To the best of the author’s knowledge, this is the first treatment of climate change from the perspective of corporate reputation.
Attractions of Dubai and Expo 2020: an exploratory study
Authors: Haneef Sunitha K. , Ansari Zakiya , Bhavani Ganga 
Published: May 2019

Purpose
The purpose of this study is to provide context to the evolution of tourist attractions in Dubai, retrospectively learn from the experiences of the Milan and Shanghai Expos and to highlight the tourist attractions of Dubai Expo 2020. In so doing, the paper also seeks to understand how attractions play a role in shaping the growth of the tourism and hospitality sectors in Dubai.

Design/methodology/approach
Data for this paper were gathered from primary and secondary sources. Informal discussions with officials of Expo 2020 generally and tourism stakeholders, in particular, who were willing to participate in this study, form the core of the primary data reflected in this paper. These discussions, which span a period of four weeks, were transcribed for later study and analysis.

Findings
First, the tourism industry, the bedrock of Dubai’s economy, has witnessed remarkable growth during the period 1990-2015 and beyond because of its rich tourism infrastructure comprising hotels, tourism activities and tourist attractions. Second, it is important that Dubai Expo draws on the experiences and expectations of prior events and can apply lessons learnt from the Milan and Shanghai Expo events. Third, that Dubai Expo 2020 can enable Dubai to build on its established image for excellent infrastructure, attractions, hotels, affordable amenities, easy accessibility and highly-developed air and road transport systems.

Research limitations/implications
Even if lessons learnt from the Milan and Shanghai Expo are taken into account, they may not account for unforeseen circumstances and sources of failure – though they provide a guide in relation to the conduct of a mega event.

Practical implications
Dubai Expo 2020 can enable Dubai to build on its established and broadening global appeal.

Social implications
Employment prospects will be enhanced by Expo 2020 that will project an image of Dubai as a world tourist destination to a far greater extent than it has done so to date. Social changes may also occur because of cultural exchanges during the Expo 2020 period.

Originality/value
The paper discusses how strategic planning for new attractions combined with the existing attractions will help spread awareness of Expo 2020. The observations made from this study can be used by other nations hosting similar events in similar geographical areas, to help prepare and draw on prior experience and lessons learnt.
SOLOMO – are hospitality SMEs able to move beyond traditional websites in their digital marketing roadmap for Expo 2020?
Authors: Kriechbaumer Florian 
Published: May 2019

Purpose
This paper aims to provide an overview of how trends encompassing SOLOMO (social, local, mobile) factors impact marketing activities in small and medium-sized hospitality enterprises (SMEs). It explores the relevancy of these factors and their application in the industry and synthesizes them in the context of Dubai’s path toward Expo 2020 by collecting practitioner input.

Design/methodology/approach
This paper provides continuity with the author’s previous WHATT article exploring SME website implementation by expanding its scope by analyzing literature on SOLOMO adoption in the hospitality industry, drawing from a range of journals and applicable industry sources. It collects informal commentary from practitioners with relevant local experience to contextualize the findings within Dubai’s digital roadmap.

Findings
Several aspects of SOLOMO in the context of hospitality have been examined by researchers; however, their successful applicability to the SME sector (which is still struggling with the fundamentals of website implementation) lacks comprehensive consideration. There is a dearth of academic and governmental guidance for industry executives. At the same time, trends beyond SOLOMO, particularly around data analytics, are emerging and require incorporation into a digital SME roadmap.

Practical implications
Executives in the hospitality SME domain are encouraged to continue focusing on the fundamentals of website implementation while they take into consideration the trends around SOLOMO identified in the study. A holistic, result-driven view of digital marketing is required to ensure focused resource usage. Closer collaboration between private sector, policymakers and academia will support Dubai’s initiatives to leverage its high degree of technological readiness for marketing innovation in the SME sector.

Originality/value
This manuscript provides a practically oriented and engaging overview of the applicability of SOLOMO concepts for hospitality SMEs, with a local focus on the Dubai ecosystem.
Development of an aid tool for evaluation of unsaturated soils permeability
Authors: Mechkarini Leila , Messafer Tahar , Bali Abderrahim , Silhadi Kamel 
Published: May 2019

Purpose
Prediction models for the unsaturated permeability proposed in the literature are numerous. However, a model may give a good result for a sample of a given soil when it may give a bad result for another sample belonging to the same type of soil. This showed that the choice of a model to complete the permeability curve in the unsaturated state is complex. To facilitate such studies, this paper aims to present a help system capable of defining the mathematical model to the user that best represents the permeability of the soil.

Design/methodology/approach
The authors have detailed the difficulties in determining the correct value of kuns from a thorough bibliographic study. To develop this idea, the authors took real examples, to which they applied mathematical models and then compared their results with those of the bibliographic study. Knowledge structuring in the form of classes, rules and functions. Implementation of the data in generator of help system Kappa-pc. validation of results.

Findings
An aid tool was developed for the evaluation of unsaturated soils permeability using Brooks and Corey (1964) and Leong and Rahardjo (1997) models, which are known for their effectiveness and ease of application. This system will also evaluate these two methods using estimation models of saturated permeability [Dane and Pocket (1992), Terzaghi (1981) and laboratory data]. This system allows the evaluation of unsaturated permeability by the aforementioned two models, makes comparison between these two models, classifies them and proposes the model presenting the best result.

Originality/value
This aid system is able to compare results of different models of prediction of the hydraulic conductivity of unsaturated soils according to several criteria (suction, degree of saturation, plasticity index, models of estimation of the permeability to the soil, saturated state, particle size, etc.). It can also deduce the model that best adapts to a given soil. This aid system will be of great use for geotechnical engineers and researchers in the field.
Severe dermatitis, multiple allergies and metabolic wasting (SAM) syndrome caused by de novo mutation in the DSP gene misdiagnosed as generalized pustular psoriasis and treatment of acitretin with gabapentin
Authors: Liang Jianying , Li Chunxiao , Zhang Zhen , Ni Cheng , Yu Hong , Li Ming , Yao Zhirong 
Published: May 2019

AbstractSevere dermatitis, multiple allergies and metabolic wasting (SAM) syndrome is a recently recognized syndrome caused by mutations in the desmoglein 1 (DSG1) and desmoplakin (DSP) genes. Only two cases of SAM‐DSP have been reported. We report on a 2‐year‐old girl presenting with pustular lakes within areas of erythema and large accumulations of intraepidermal neutrophils, which initially led to our misdiagnosis of generalized pustular psoriasis. No mutation was found in either the IL36RN or CARD14 genes by Sanger sequencing. The distinctive manifestations of erythroderma with severe itching, hypotrichosis, enamel defects, onychodystrophy, palmoplantar keratoderma and the crucial result of de novo missense mutation in exon 14 of the DSP gene (c.1828T>C, p.S610P) discovered by next‐generation sequencing finally confirmed the diagnosis of SAM syndrome. The eruptions significantly improved after a 4‐week treatment with oral acitretin and topical pimecrolimus. Oral gabapentin was prescribed simultaneously for 4 months, relieving her skin pruritus and suggesting that early treatment with pimecrolimus, acitretin and gabapentin for SAM‐DSP syndrome is effective. It may even inhibit multiple allergies induced by skin barrier injury. In this work we also review the clinical features, differential diagnoses and pathological manifestations of SAM‐DSP syndrome.
A genomic island in a plant beneficial rhizobacterium encodes novel antimicrobial fatty acids and a self‐protection shield to enhance its competition
Authors: Wang Dandan , Xu Zhihui , Zhang Guishan , Xia Liming , Dong Xiaoyan , Li Qing , Liles Mark R. , Shao Jiahui , Shen Qirong , Zhang Ruifu 
Published: May 2019

SummaryRhizobacteria devote a relatively large percentage of their genomes to encode bioactive natural products that are important for competition in the rhizosphere. In this study, a plant beneficial rhizobacteriumBacillus velezensisSQR9 was discovered to produce novel antibacterial fatty acids, Bacillunoic acids, which are encoded on a genomic island (GI). This GI contains a hybrid type I fatty acid synthase (FAS)‐polyketide synthase (PKS) system and an ABC transporter. The FAS was predicted to synthesize a primer that was transferred to the PKS to synthesize Bacillunoic acids. The synthesized Bacillunoic acids inhibit the growth of diverse bacteria, with the strongest activity against closely relatedBacillusstrains, the ABC transporter exported the toxic Bacillunoic acids upon their induction for protecting the producing strain. The inhibition of otherBacillusstrains by Bacillunoic acids extended the antimicrobial spectrum of SQR9 and enhanced its competition with closely related root‐associated bacteria. So, through the obtaining of this GI by horizontal gene transfer, strain SQR9 not only acquired a competitive weapon but also acquired a self‐protecting shield, which increased its competition with other rhizobacteria.
Multi‐year dynamics of fine‐scale marine cyanobacterial populations are more strongly explained by phage interactions than abiotic, bottom‐up factors
Authors: Ahlgren Nathan A. , Perelman Jessica N. , Yeh Yi‐Chun , Fuhrman Jed A. 
Published: May 2019

SummaryCurrently defined ecotypes in marine cyanobacteria Prochlorococcus and Synechococcus likely contain subpopulations that themselves are ecologically distinct. We developed and applied high‐throughput sequencing for the 16S‐23S rRNA internally transcribed spacer (ITS) to examine ecotype and fine‐scale genotypic community dynamics for monthly surface water samples spanning 5 years at the San Pedro Ocean Time‐series site. Ecotype‐level structure displayed regular seasonal patterns including succession, consistent with strong forcing by seasonally varying abiotic parameters (e.g. temperature, nutrients, light). We identified tens to thousands of amplicon sequence variants (ASVs) within ecotypes, many of which exhibited distinct patterns over time, suggesting ecologically distinct populations within ecotypes. Community structure within some ecotypes exhibited regular, seasonal patterns, but not for others, indicating other more irregular processes such as phage interactions are important. Network analysis including T4‐like phage genotypic data revealed distinct viral variants correlated with different groups of cyanobacterial ASVs including time‐lagged predator–prey relationships. Variation partitioning analysis indicated that phage community structure more strongly explains cyanobacterial community structure at the ASV level than the abiotic environmental factors. These results support a hierarchical model whereby abiotic environmental factors more strongly shape niche partitioning at the broader ecotype level while phage interactions are more important in shaping community structure of fine‐scale variants within ecotypes.
Efficient colonization of the endophytes Herbaspirillum huttiense RCA24 and Enterobacter cloacae RCA25 influences the physiological parameters of Oryza sativa L. cv. Baldo rice
Authors: Andreozzi Anna , Prieto Pilar , Mercado‐Blanco Jesús , Monaco Stefano , Zampieri Elisa , Romano Silvia , Valè Gianpiero , Defez Roberto , Bianco Carmen 
Published: May 2019

SummarySeveral important bacterial characteristics, such as biological nitrogen fixation, phosphate solubilization, 1‐aminocyclopropane‐1‐carboxylate deaminase activity and production of siderophores and phytohormones, can be assessed as plant growth promotion traits. Our aim was to evaluate the effects of nitrogen fixing and indole‐3‐acetic acid (IAA) producing endophytes in two Oryza sativa cultivars (Baldo and Vialone Nano). Three bacteria, Herbaspirillum huttiense RCA24, Enterobacter asburiae RCA23 and Staphylococcus sp. 377, producing different IAA levels, were tested for their ability to enhance nifH gene expression and nitrogenase activity in Enterobacter cloacae RCA25. Results showed that H. huttiense RCA24 performed best. Improvement in nitrogen fixation and changes in physiological parameters such as chlorophyll, nitrogen content and shoot dry weight were observed for plants co‐inoculated with strains RCA25 and RCA24 in a 10:1 ratio. Based on confocal laser scanning microscopy analysis, strain RCA24 was the best colonizer of the root interior and the only IAA producer located in the same root niche occupied by RCA25 cells. This work shows that the choice of a bio‐inoculum having the right composition is one of the key aspects to be considered for the inoculation of a specific host plant cultivar with microbial consortia.