bluuebunny/crossref_metadata_2025_split huggingface dataset:
Involvement of GASL1 in postoperative distant recurrence of gastric adenocarcinoma after gastrectomy distal resection and the possible mechanism
Authors: Wang Bing , Chen Huawei , Zhang Yang 
Published: March 2019

AbstractAs a cancer‐related long noncoding RNA, functionality of GASL1 has only been characterized in liver cancer. Our study aimed to investigate the possible involvement of GASL1 in postoperative recurrence of gastric adenocarcinoma. A total of 112 gastric adenocarcinoma patients with a tumor located in the distal third who received gastrectomy distal resection in The Second Hospital of Dalian Medical University from January 2012 to January 2015 were included in this study. Patients were followed up for 3 years. Another 56 healthy people were also included to serve as a control group. Blood was extracted from each subject on the day of discharge, on the day of diagnosis of recurrence or at the end of follow‐up. The GASL1 expression vector was transfected into gastric adenocarcinoma cell lines. It was observed that plasma levels of GASL1 were significantly lower, while plasma levels of transforming growth factor‐β1 (TGF‐β1) were significantly higher in patients than in healthy controls on the day of discharge. Patients with distant recurrence showed significantly lower plasma levels of GASL1 and significantly higher plasma levels of TGF‐β1 compared with patients with local recurrence and patients without recurrence. During follow‐up, plasma levels of GASL1 and TGF‐β1 were negatively correlated in patients with distant recurrence but not in other groups of patients. GASL1 overexpression inhibited, while TGF‐β1 treatment promoted cell migration and invasion. Overexpression of GASL1 led to downregulated and GASL1 knockdown led to upregulated TGF‐β1. However, TGF‐β1 showed no significant effects on GASL1 expression. We conclude that GASL1 may participate in the distant recurrence of gastric adenocarcinoma through the interactions with TGF‐β1.
MicroRNA‐19a‐3p promotes rheumatoid arthritis fibroblast‐like synoviocytes via targeting SOCS3
Authors: Chen Yiyue , Wang Wanchun , Chen You , Tang Qi , Zhu Weihong , Li Ding , Liao Lele 
Published: March 2019

AbstractRheumatoid arthritis (RA) is a common chronic autoimmune disease and effective treatment for RA is still lacking. In this study, the regulatory role of miR‐19a‐3p in RA was investigated. Quantitative polymerase chain reaction analysis of human blood samples showed that the level of miR‐19a‐3p was significantly lower in the RA patients compared with that in healthy patients (P < 0.05). In RA fibroblast‐like synoviocytes (RAFLS), miR‐19a‐3p and suppressor of cytokine signaling 3 (SOCS3) were also downregulated and upregulated, respectively, compared with those of normal FLS. Transfection of miR‐19a‐3p mimic in RAFLS inhibited cell proliferation and promoted cell apoptosis. TargetScan identified SOCS3 as a target of miR‐19a‐3p, which was confirmed by dual‐luciferase assay. Western blot indicated that SOCS3 protein level was significantly decreased after miR‐19a‐3p overexpression. Moreover, SOCS3 silencing through siRNA transfection also enhanced cell proliferation, meanwhile inhibiting RAFLS apoptosis. In addition, SOCS3 overexpression abrogated the effects of miR‐19a‐3p overexpression on cell proliferation and apoptosis, corroborating that SOCS3 acts as a downstream effector in the miR‐19a‐3p‐mediated function of RAFLS. These findings suggest that miR‐19a‐3p plays an important role in RA, and the miR‐19a‐3p/SOCS3 axis may become a potential therapeutic target for RA.
Rhoifolin ameliorates titanium particle‐stimulated osteolysis and attenuates osteoclastogenesis via RANKL‐induced NF‐κB and MAPK pathways
Authors: Liao Shijie , Song Fangmin , Feng Wenyu , Ding Xiaofei , Yao Jun , Song Huijie , Liu Yun , Ma Shiting , Wang Ziyi , Lin Xixi , Xu Jiake , Zhao Jinmin , Liu Qian 
Published: March 2019

AbstractProsthesis loosening is a highly troublesome clinical problem following total joint arthroplasty. Wear‐particle‐induced osteoclastogenesis has been shown to be the primary cause of periprosthetic osteolysis that eventually leads to aseptic prosthesis loosening. Therefore, inhibiting osteoclastogenesis is a promising strategy to control periprosthetic osteolysis. The possible mechanism of action of rhoifolin on osteoclastogenesis and titanium particle‐induced calvarial osteolysis was examined in this study. The in vitro study showed that rhoifolin could strongly suppress the receptor activators of nuclear factor‐κB (NF‐κB) ligand‐stimulated osteoclastogenesis, hydroxyapatite resorption, F‐actin formation, and the gene expression of osteoclast‐related genes. Western blot analysis illustrated that rhoifolin could attenuate the NF‐κB and mitogen‐activated protein kinase pathways, and the expression of transcriptional factors nuclear factor of activated T cells 1 (NFATc1) and c‐Fos. Further studies indicated that rhoifolin inhibited p65 translocation to the nucleus and the activity of NFATc1 and NF‐κB rhoifolin could decrease the number of tartrate‐resistant acid phosphate‐positive osteoclasts and titanium particle‐induced C57 mouse calvarial bone loss in vivo. In conclusion, our results suggest that rhoifolin can ameliorate the osteoclasts‐stimulated osteolysis, and may be a potential agent for the treatment of prosthesis loosening.
Quercetin attenuates oxidative stress‐induced apoptosis via SIRT1/AMPK‐mediated inhibition of ER stress in rat chondrocytes and prevents the progression of osteoarthritis in a rat model
Authors: Feng Kai , Chen Zhaoxun , Pengcheng Liu , Zhang Shuhong , Wang Xiaoqing 
Published: March 2019

AbstractApoptosis of chondrocytes are the main initiator of osteoarthritis (OA) and can be explained by oxidative stress and endoplasmic reticulum (ER) stress, thus the pharmacological interventions aimed at inhibiting of these pathways may be a promising approach for the management of OA. Quercetin is a member of the flavonoid family and has antioxidant and anti‐inflammatory properties in degenerative diseases. However, its effects and potential mechanisms on the pathological process of OA are not very clear. The present study aimed to investigate the protective effects of quercetin on OA and the underlying mechanisms. The tert‐butyl hydroperoxide (TBHP)‐stimulated rat chondrocytes and destabilization of the medial meniscus OA rat model was used to explore the protective effects of quercetin. Our results showed that quercetin treatment can attenuate oxidative stress, ER stress, and associated apoptosis. Moreover, quercetin inhibited ER stress through activating the sirtuin1/adenosine monophosphate‐activated protein kinase (SIRT1/AMPK) signaling pathway. The protective effects of quercetin were also observed in OA rat model which is evidenced by abolished cartilage degeneration and decreased chondrocytes apoptosis in the knee joints. Our results suggested that quercetin is a promising treatment for OA.
Novel trastuzumab‐DM1 conjugate: Synthesis and bio‐evaluation
Authors: Abedi Mehri , Cohan Reza Ahangari , Mahboudi Fereidoun , Faramarzi Mohammad Ali , Fazel Ramin , Damavandi Narges , Ardestani Mehdi Shafiee , Davami Fatemeh 
Published: March 2019

AbstractAntibody‐drug conjugates are now of considerable interest and are recommended for the treatment of cancers. Linkers are having a crucial role in potency and efficacy of these drugs. Herein, for the first time, we have used a water‐soluble poly‐ethylene glycol based linker (succinimidyl‐[(N‐maleimido propionamido)‐diethyleneglycol] [SM(PEG)2]) for lysine amide coupling of DM1 drug to trastuzumab considering evaluation of the effect of using a hydrophilic linker on physicochemical and biological properties of the resulting conjugate in comparison to the conjugate containing succinimidyl 4‐(N‐maleimidomethyl) cyclohexane‐1‐carboxylate (SMCC) linker, which has a relative hydrophobic nature. The physicochemical properties of synthesized conjugates were investigated in terms of drug to antibody ratio, size variants and free drug quantities. In vitro biological activity of trastuzumab‐DM1 conjugates was assessed on breast cancer cell lines expressing different levels of HER2 using binding affinity, antiproliferative, apoptosis, and antibody‐dependent cell‐mediated cytotoxicity (ADCC) assays. Synthesized conjugate containing hydrophilic linker, showed higher drug to antibody ratio, no aggregated form and higher cellular toxicity in comparison to SMCC bearing conjugate. Binding affinity and ADCC potential of conjugates was not affected upon the usage of hydrophilic linker. In conclusion, application of SM(PEG)2 for coupling of DM1 to trastuzumab enhance desirable characteristics of the resulting conjugate.
Pancreas deformation in the presence of tumors using feature tracking from free‐breathing XD‐GRASP MRI
Authors: Chitiboi Teodora , Muckley Matthew , Dane Bari , Huang Chenchan , Feng Li , Chandarana Hersh 
Published: March 2019

BackgroundQuantifying the biomechanical properties of pancreatic tumors could potentially help with assessment of tumor aggressiveness, prognosis, and prediction of therapy response.PurposeTo quantify respiratory‐induced deformation in the pancreas and pancreatic lesions using XD‐GRASP (eXtra‐Dimensional Golden‐angle RAdial Sparse Parallel), MRI.Study TypeRetrospective study where patients undergoing clinically indicated abdominal MRI which included free‐breathing radial T1‐weighted (T1W) imaging were studied.SubjectsThirty‐two patients (12 male and 20 female) including nine with pancreatic lesions constituted our study cohort.Field Strength/Sequence3.0 T with T1WI contrast‐enhanced gradient echo radial free‐breathing acquisition.AssessmentUsing the XD‐GRASP imaging technique, the acquired free‐breathing radial data were sorted and binned into 10 consecutive respiratory motion states that were jointly reconstructed. 3D deformation fields along the respiratory dimension were computed using an optical flow method and were analyzed in the pancreas.Statistical TestsThe Wilcoxon signed‐rank test was used to assess the difference in average displacement across pancreatic regions, while the Wilcoxon rank‐sum test was used for displacement differences between patients with and without tumors. The interclass correlation coefficient (ICC) was computed to assess consistency between observers for each image quality measure.ResultsThere was a significantly larger displacement in the pancreatic tail compared with the head (8.2 ± 3.7 mm > 5.8 ± 2.4 mm; P < 0.001) and body regions (8.2 ± 3.7 mm > 6.6 ± 2.9 mm; P < 0.001). Furthermore, there was reduced normalized average displacement in patients with pancreatic lesions compared with subjects without lesions (0.33 ± 0.1 < 0.69 ± 0.26, P < 0.001 for the head; 0.30 ± 0.1 < 0.84 ± 0.31, P < 0.001 for the body; and 0.44 ± 0.31 < 1.08 ± 0.53, P < 0.001 for the tail, respectively).Data ConclusionFree‐breathing respiratory motion‐sorted XD‐GRASP MRI has the potential to noninvasively characterize the biomechanical properties of the pancreas by quantifying breathing‐induced mechanical displacement.Level of Evidence: 4Technical Efficacy: Stage 2J. Magn. Reson. Imaging 2019;50:1633–1640.
Diurnal variability in alkaline phosphatase activity and the potential role of zooplankton
Authors: Davis C. , Lohan M. C. , Tuerena R. , Cerdan‐Garcia E. , Woodward E. M. S. , Tagliabue A. , Mahaffey C. 
Published: March 2019

AbstractDaily light–dark cycles drive the circadian rhythm of many ocean processes including photosynthesis, gene expression, and zooplankton diel vertical migration (DVM). In phosphate deplete surface ocean regions, microbes produce metalloenzymes, such as alkaline phosphatases (AP), to access dissolved organic phosphorus. Here, we provide novel evidence of diurnal variation in AP activity (APA) in the subtropical North Atlantic using two independent datasets, with APA being two‐ to three‐fold higher at night. We demonstrate that zooplankton are a source of AP and postulate that zooplankton DVM is a source of enhanced AP in the surface waters at night, with reduction or degradation of AP during the day. Our results challenge the current assumption that APA is linear over a 24‐h period. While future ocean scenarios predict intensification and expansion of oceanic phosphate limitation, our findings indicate a role for zooplankton in regenerating phosphate that is currently missing in conceptual and numerical models.
Exact analysis of (R, s, S) inventory control systems with lost sales and zero lead time
Authors: Esmaili Nazanin , Norman Bryan A. , Rajgopal Jayant 
Published: March 2019

AbstractWe study an (R, s, S) inventory control policy with stochastic demand, lost sales, zero lead‐time and a target service level to be satisfied. The system is modeled as a discrete time Markov chain for which we present a novel approach to derive exact closed‐form solutions for the limiting distribution of the on‐hand inventory level at the end of a review period, given the reorder level (s) and order‐up‐to level (S). We then establish a relationship between the limiting distributions for adjacent values of the reorder point that is used in an efficient recursive algorithm to determine the optimal parameter values of the (R, s, S) replenishment policy. The algorithm is easy to implement and entails less effort than solving the steady‐state equations for the corresponding Markov model. Point‐of‐use hospital inventory systems share the essential characteristics of the inventory system we model, and a case study using real data from such a system shows that with our approach, optimal policies with significant savings in inventory management effort are easily obtained for a large family of items.
Developing a culturally tailored multigenerational intervention to prevent and manage type 2 diabetes in American Indian families living in rural settings: Findings from a focus group study
Authors: Scarton Lisa , Velazquez Ilse , O'Neal LaToya J. , Iyerm Samvit , Cannady Tamela , Choate Annette , Mitchell Cayla , Wilkie Diana J. 
Published: March 2019

AbstractThe study purpose was to understand the characteristics of interventions that would be most relevant and beneficial to address the diabetes‐related needs and challenges of rural American Indians/Alaska Natives (AIAN) with type 2 diabetes (T2D) and their families. In an exploratory study design, we held a total of seven focus groups in Florida and rural Oklahoma. Groups included 3–13 individuals (62 total, 77% were female, mean age 55.3 [11.4] years and mean duration of diabetes 10.4 [SD 9.1] years) who were referred by staff from HealthStreet, Consent2Share mechanism, and by tribal educators. All groups were moderated by the same American Indian research team member using a discussion guide with open‐ended questions, followed by probes. Findings revealed themes centered on optimal intervention components, barriers to type 2 diabetes—prevention and management (T2D‐PM), personal experiences with T2D, and impact of family behaviors on T2D‐PM. Findings indicate that the participants desire diabetes programs that include family members and a hands‐on, culturally meaningful approach. Creating an intervention based on the AIAN community's insights that include the entire family may improve T2D‐PM outcomes for this population.
Effects of a primary palliative care intervention on quality of life and mental health in cystic fibrosis
Authors: Friedman Deborah , Linnemann Rachel W. , Altstein Lily L. , Georgiopoulos Anna M. , Islam Suhayla , Bach Kieu‐Tram , St. John Anita , Fracchia M. Shannon , Neuringer Isabel , Lapey Allen , Sicilian Leonard , Moskowitz Samuel M. , Yonker Lael M. 
Published: March 2019

AbstractBackgroundDespite the significant impact of chronic symptoms on quality of life with cystic fibrosis (CF), the role of palliative care in management of this disease is not well defined. The coping, goal assessment, and relief from evolving CF symptoms (CF‐CARES) model is a primary palliative care intervention designed to provide chronic symptom management at all stages of the disease. The goal of this pilot study was to estimate the effectiveness of the CF‐CARES intervention on improving chronic symptoms and quality of life for people living with CF.MethodsA structured assessment was used to guide referral to supportive services intended to address burdensome symptoms. Follow‐up assessments were performed approximately 3 and 6 months later. Longitudinal regression analyses of changes in symptoms and quality of life were performed for all participants regardless of utilization of supportive services. Subgroup analyses were performed for subjects participating in mental health and alternative health services.ResultsForty‐one subjects completed assessment and referral processes. The mean number of CF‐associated symptoms decreased over time, as did respiratory symptom‐related distress and depressive symptoms. Subjects utilizing alternative health services reported less psychological distress at follow‐up. Among subjects with severe disease, mental health, and quality of life improved, especially for those using mental health services.ConclusionsThe CF‐CARES model resulted in significant mental health and quality‐of‐life benefits, suggesting the value of integrating symptom management interventions into routine CF care. Moreover, mental health services can play a key role in CF‐specific primary palliative care, especially for those with advanced disease.
Endothall behavior in Myriophyllum spicatum and Hydrilla verticillata
Authors: Ortiz Mirella F , Nissen Scott J , Gray Cody J 
Published: March 2019

AbstractBACKGROUNDEndothall has been used to control submersed aquatic plants since 1960, providing broad‐spectrum control of aquatic weeds. Although endothall is considered a contact herbicide, many field observations suggest that it might have systemic activity. The goals of this research were to determine endothall's (i) absorption characteristics, (ii) translocation from shoots to roots, and (iii) potential for desorption in Eurasian watermilfoil (EWM), monoecious and dioecious hydrilla.RESULTSEndothall absorption was linear in dioecious hydrilla up to 192 HAT, while in EWM and monoecious hydrilla absorption data best fit an asymptotic rise function. Endothall absorption in EWM, monoecious and dioecious hydrilla was 3.3, 6.6, and 11.0 times the external herbicide concentration determined by the plant concentration factor. Translocation to EWM roots reached 7.9% of total absorbed radioactivity by 192 HAT, while translocation to monoecious and dioecious hydrilla roots reached 17.8% and 16.4% by 192 HAT, respectively. For all three species, no more than 30% of absorbed endothall moved from the plant to clean water 96 HAT.CONCLUSIONEndothall is a very water soluble compound and yet it accumulated in these three important aquatic weeds at concentrations significantly higher than the external herbicide concentration. These data provide evidence that endothall could have systemic activity in these aquatic species. Following 14C‐endothall applications, more 14C translocated from shoots to roots compared to the translocation of 14C for other systemic aquatic herbicides. The final confirmation of endothall's systemic behavior requires that the radioactivity found in the root system of these aquatic plants is 14C endothall. © 2019 Society of Chemical Industry
Self-esteem and cyberchondria: The mediation effects of health anxiety and obsessive–compulsive symptoms in a community sample
Authors: Bajcar Beata , Babiak Jolanta 
Published: March 2019

AbstractCyberchondria refers to the excessive and repeated searching for medical information on the Internet and may be considered as health-related problematic Internet use. Previous findings indicated that cyberchondria is positively associated with health anxiety and obsessive–compulsive symptoms. Also, research suggests that excessive or problematic Internet use as well as health worries and compulsive behaviors are present among individuals with low self-esteem. This study sought to examine: (1) the association between self-esteem and cyberchondria, and (2) the mediating role of health anxiety and obsessive–compulsive symptoms in the relationship between self-esteem and cyberchondria. Participants (N = 207) from a community sample completed self-report measures assessing global self-esteem, health anxiety, obsessive–compulsive symptoms, and cyberchondria. We found that self-esteem directly predicted cyberchondria and that health anxiety and obsessive–compulsive symptoms parallelly mediated the relationship between self-esteem and cyberchondria. These findings suggest that low self-esteem, health anxiety and obsessive–compulsive symptoms can be considered vulnerability factors for cyberchondria. In addition, the reverse mediation model indicated that cyberchondria potentially predicts self-esteem both directly and through health anxiety and obsessive–compulsive symptoms. The bidirectional relationship among the analyzed variables are discussed in the context of potential psychological predictors and consequences of cyberchondria and possible mechanisms explaining cyberchondria. The current study provides further insight into the conceptualization of cyberchondria and the feasibility of specific treatment directions.
Assessment of Groundwater Pollution Vulnerability Using GIS Based Modified DRASTIC Model in Raipur City, Chhattisgarh
Authors: Khan Rubia , Jhariya D. C. 
Published: March 2019

Abstract
In the present study DRASTIC model was used to assess the groundwater vulnerable zone for Raipur city. In this study DRASTIC model is modified into four ways i.e. DRASTIC, Modified DRASTIC-Lu, DRASTIC AHP and Modified DRASTIC-Lu AHP. To modify the DRASTIC model, LULC parameter was added in the real DRASTIC model and also AHP technique applied to determine the rating and weight. In this study, it is observed that 2.83%, 7.57%, 32.03%, 47.78% and 9.8% areas are respectively falling under very low, low, moderate, high and very high vulnerable DRASTIC index, 2.56%, 10.96%, 30.10%, 47.47% and 8.9% areas are respectively falling under very low, low, moderate, high and very high vulnerable modified DRASTIC-Lu index, 3.27%, 16.63%, 47.14%, 74.44% and 9.96% area are respectively falling under very low, low, moderate, high and very high vulnerable DRASTIC-AHP index classes and 2.74%, 12.27%, 38.16%, 41.5% and 5.3% area are respectively falling under very low, low, moderate, high and very high vulnerable modified DRASTIC-Lu AHP index. To determine the accuracy of the DRASTIC models, total 50 groundwater samples of nitrate concentration were used for pre-monsoon and post-monsoon seasons and it was observed that Modified DRASTIC-Lu AHP model is most accurate and suitable for present study area.