bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neuroinvasive virus utilizes a lipid droplet surface protein, perilipin2, to restrict apoptosis by decreasing Bcl-2 ubiquitination
Authors: Wang Qianruo , Zhao Jianqing , Zhang Mai , Sun Meixin , Fu Zhen F. , Zhao Ling , Zhou Ming 
Published: November 2024

ABSTRACT

Lipid droplets (LDs) can interact with other organelles to regulate cell death, and it has also been reported to play an important role in virus replication. However, the interplay among LDs, cell death, and viral replication remains unclear. Neuroinvasive viruses, such as Japanese encephalitis virus (JEV), rabies virus (RABV), and encephalomyocarditis virus (EMCV) still threaten global public health and raise intensive concerns. Here, we reveal that neuroinvasive virus infection enhances cellular triglyceride biosynthesis by upregulating the expression of diacylglycerol O-acyltransferase 2 (DGAT2) to promote LD formation and increase the expression of Perilipin 2 (PLIN2), an LD surface protein, which consequently facilitates neuroinvasive virus replication. Furthermore, PLIN2 could reduce mitochondrial damage and suppress apoptosis by restoring mitochondrial potential and interacting with anti-apoptotic protein Bcl-2, specifically the 136–209 amino acid region, to interrupt the BAX-Cytc-caspase-3 apoptotic pathway by decreasing the K48-linked ubiquitination of Bcl-2 at the 17th lysine. Together, we elucidate that neuroinvasive virus utilizes an LD surface protein to restrict the apoptosis of infected cells, providing a fresh insight into the pathogenesis and antiviral therapeutics development of neuroinvasive viruses.

IMPORTANCE
The neuroinvasive virus is a kind of pathogen that is capable of infiltrating and infecting the central nervous system to potentially induce severe neurological damage and disorders, which pose a significant threat to public health. Here, we found that neuroinvasive viruses can utilize an LD surface protein PLIN2 to facilitate viral replication. Notably, PLIN2 could reduce mitochondrial damage and suppress apoptosis by restoring mitochondrial potential and interacting with anti-apoptotic protein Bcl-2, specifically the 136-209 amino acid region, to interrupt the BAX-Cytc-caspase-3 apoptotic pathway by decreasing the K48-linked ubiquitination of Bcl-2 at the 17th lysine. This study reveals a common strategy for neuroinvasive viruses to avoid apoptosis of infected cells by employing LDs, which extends the important role of LDs in viral pathogenesis and may inspire further research in this field.
Lethal model for respiratory syncytial virus infection using C57BL/6 mice
Authors: Takahashi Tatsuki , Amarbayasgalan Sodbayasgalan , Ueno Shiori , Sugiura Yoshiro , Dorjsuren Enkhjin , Shimizu Kenta , Kamitani Wataru 
Published: November 2024

ABSTRACT

Respiratory syncytial virus (RSV) infection is a major infectious disease affecting public health. Infants and elderly infected with RSV can develop severe respiratory symptoms. A mouse model mimicking human RSV infection could be useful in elucidating the pathogenesis of RSV. However, previous mouse models did not adequately mimic the pathophysiology of human patients. We attempted to establish a new mouse-adapted RSV strain via serial passaging of mice. We rescued the MP11 virus (which had one non-synonymous substitution in each of the F, G, and L genes) through serial passaging in mice. The MP11 virus was inoculated into mice to evaluate whether it had adapted to the mouse. Viral RNA levels in the lungs of 25-week-old mice infected with MP11 virus were higher than those in the lungs of mice infected with A2 virus. There was a high infiltration of inflammatory cells and high expression of several inflammatory cytokines (IFN-γ, CCL2, TNF-α, and IL-6) in the MP11 virus-infected lungs. Furthermore, the MP11 virus can also cause lethal pneumonia in mice via high-concentration inoculation. These results indicated that the MP11 virus is a more mouse-adapted strain than the A2 virus. We generated a recombinant MP11 virus (rMP11) using reverse genetics. The rMP11 virus could grow in the lungs of mice, similar to the parent MP11 virus. In conclusion, we successfully established a new mouse-adapted strain, MP11, and reverse genetics for this strain. These MP11 and rMP11 viruses could contribute to mouse experiments aimed at elucidating RSV pathogenesis.

IMPORTANCE
A mouse model of respiratory syncytial virus (RSV) infection is useful for fundamental research aimed at developing antiviral drugs. Previous mouse models of RSV infection were unable to adequately mimic the pathophysiology of human patients due to the low amplification efficiency of this virus in the mouse lung. Furthermore, mice other than BALB/C mice are difficult to use for the RSV infectious model. We established a new mouse-adapted RSV strain, MP11. The MP11 virus can cause severe pneumonia in C57BL/6 mice and efficiently replicate and induce inflammation in the lung. Therefore, C57BL/6 mice can be used for RSV infection experiments using MP11 virus. We established a reverse genetics system for the MP11 virus using our mouse model. This system enables detailed analyses of the MP11 virus, such as functional analysis of each viral protein. Our study provides techniques that can advance fundamental research in elucidating the pathogenesis of RSV infections.
Genome sequences of
Microvirga
spp. CF3062 and CF3016 isolated from nodules found on herbarium specimens collected in 2004 and 2015
Authors: Petipas Renee H. , Kehlet-Delgado Hanna , Antoch Amanda A. , Friesen Maren L. 
Published: November 2024

ABSTRACT

We present the genomes of two
Microvirga
isolates isolated from nodules found on
Medicago lupulina
herbarium specimens at the Marion Ownbey Herbarium at Washington State University. These genomes and others from herbarium specimens offer an unprecedented opportunity to study the bacterial evolution of plant-associated microbes over long time scales.
Geochemical evidence for terrigenous sediment recycling in the Late Permian subcontinental lithospheric mantle of the northern North China Craton
Authors: Wang Zhigang , Dai Li-Qun , Zhao Zi-Fu , Fang Wei 
Published: November 2024

Abstract
Understanding the recycling process of subducted slab in subduction zones is vital to deciphering the heterogeneity of cratonic mantle and the variable compositions of continental arc igneous rocks. This study presents a comprehensive analysis of zircon U-Pb ages, Hf-O isotopes, hornblende major elements, whole-rock major and trace elements, as well as Sr-Nd-Hf isotopes of mafic igneous rocks from the northern North China Craton. These data constrain metasomatic processes in the cratonic mantle. The Late Permian mafic igneous rocks (ca. 254–252 Ma) studied are characterized by arc-like trace element signatures and enriched whole-rock Sr-Nd-Hf isotopes, with (87Sr/86Sr)i ratios of 0.7063–0.7076, εNd(t) values of −18.0 to −9.3, and εHf(t) values of −29.7 to +0.5. In addition, they also have elevated zircon δ18O values of 5.9‰–7.0‰, and variable zircon εHf(t) values of −19.4 to +6.0. These features suggest the rocks were derived from an enriched mantle with the involvement of terrigenous sediments. We propose that the subcontinental lithospheric mantle of the northern North China Craton was mainly metasomatized by terrigenous sediment-derived hydrous melt during the southward subduction of the Paleo-Asian Ocean. Moreover, partial melting of the metasomatic mantle may be triggered by the slab rollback related to the closure of the Paleo-Asian Ocean in the Late Permian, which resulted in the formation of the mafic igneous rocks studied. Thus, the Late Permian igneous rocks studied provide petrological and geochemical evidence of the crust-mantle interaction during the subduction of the Paleo-Asian Ocean.
Supplemental Material: Throw distribution across the Dabbahu–Manda Hararo dike-induced fault array: Implications for rifting and faulting
Authors: Hofmann Barbara , al. et 
Published: November 2024

<p> We provide a version of Figure 2 without interpretations to better view the data used. There is also a text file describing further detail of the datasets and methods used and an associated figure showing the ALOS interferogram of ground deformation associated with the October 2008 diking event. </p>
Association of self-reported health problems and interpersonal violence in sport: a cross-sectional study in world-level performing athletes
Authors: Mountjoy Margo , Adriaens Karolien , Junge Astrid , Verhelle Helena , Laureys Felien , Kirby Sandi , Dienstbach-Wech Lenka , Foster Jeremy , Vertommen Tine 
Published: November 2024

ObjectivesA paucity of studies assesses the intersection of physical health (injury and illness), mental health and experiences of interpersonal violence (IV, also known as harassment and abuse) in sport. The objectives of this study were to examine the (a) frequency of self-reported physical and mental health problems of elite athletes in the 12 months prior to the survey, (b) differences in physical and mental health between male and female athletes and (c) relationship of athlete health with experiences of IV.MethodsElite adult athletes from four sports were approached at eight international events to answer an online questionnaire on their physical and mental health, as well as experiences of IV in sport within the past 12 months.ResultsA total of 562 athletes completed the questionnaire. Overall, 75% reported at least one physical symptom, most commonly headache and fatigue (n=188; 33.5% each), followed by musculoskeletal symptoms (n=169; 29.4%). 65.1% reported at least one mental health symptom, mostly of anxiety or depression. More female than male athletes reported physical (F:81.9%; M:68.3%; p<0.001) and mental (F:71.9%; M:58.4%; p<0.001) health problems, while addiction problems were more frequent in male athletes (F:1.8%; M:6.4%; p=0.006. 53.0% of the female and 42.3% of the male participants reported having experienced at least one form of IV. Linear regression analysis demonstrated that all forms of IV, except physical IV (all p’s<0.001), were associated with an increasing number of physical and mental health symptoms. In addition, the analysis showed that female athletes had a higher increase in symptoms in response to IV than male athletes.ConclusionsThis study demonstrates the relationship of elite athlete physical and mental health with IV. Injury and illness prevention programmes in international sport should include strategies to reduce IV.
Shifting official development assistance during COVID-19: earmarking, donor concentration and loans
Authors: Woskie Liana , Wenham Clare 
Published: November 2024

ContextIn contrast to bilateral aid, aid disbursed from multilateral institutions increased significantly at the onset of the COVID-19 pandemic. Yet, at a time when a coherent and effective multilateral response is needed most, the COVID-19 pandemic revealed a shifting landscape of donor agencies that struggle with basic functions, such as cross-national coordination. While multilaterals are uniquely positioned to transcend national priorities and respond to pandemics, functionally we find official development assistance (ODA) from these entities may increasingly mimic the attributes of bilateral aid. We explore three important, but not comprehensive, attributes of aid leading up to and during the COVID-19 pandemic: (1) earmarking, (2) donor concentration and (3) aid modality.MethodsWe examine ODA disbursements over time in 2020 constant prices from 2010 to 2021 and plot share of inflow that is earmarked against each United Nations multilateral against their average annual financing volume. We then assess market diversity with two measures: the Shannon-Weiner Function and Gini-Simpson Index. Finally, we examine financing vehicles used to disburse and look at ‘grant share’ of total ODA from all formal donors over time.FindingsWe find that while the absolute number of formal multilateral actors and market diversity have been increasing since 2011, there has been a concurrent market consolidation led by the World Bank Group at 37% of market share in 2021. This coincides with an increasing prevalence of earmarking of aid inflows to the multilateral system and, unique to multilaterals but concerning given increasing debt risk, a rise in loan-based ODA disbursements.ConclusionsIn theory, this consolidation may streamline revenue pooling and allow for a more collective approach to mitigating pandemic risk but, paired with increased earmarking, has the potential to sideline both collective goals (eg, the Sustainable Development Goals) and counties’ core mandates (such as the pursuit of universal health coverage).
What CPAP to use in the delivery room? Bench comparison of two methods to provide continuous positive airways pressure in neonates
Authors: Gruber Viktoria , Tracy Mark Brian , Hinder Murray Kenneth , Morakeas Stephanie , Dronavalli Mithilesh , Drevhammar Thomas 
Published: November 2024

BackgroundContinuous positive airway pressure (CPAP) is a recommended first-line therapy for infants with respiratory distress at birth. Resuscitation devices incorporating CPAP delivery can have significantly different imposed resistances affecting airway pressure stability and work of breathing.AimTo compare CPAP performance of two resuscitation devices (Neopuff T-piece resuscitator and rPAP) in a neonatal lung model simulating spontaneous breathing effort at birth.MethodsThe parameters assessed were variation in delivered pressures (∆P), tidal volume (VT), inspiratory effort (model pressure respiratory muscle (PRM)) and work of breathing (WOB). Two data sequences were required with Neopuff and one with rPAP: (1) set PRM with changes in VT and (2) constant VT (preterm 6 mL, term 22 mL) with increased effort. Data were collected at CPAP settings of 5, 7 and 9 cmH2O using a 1 kg preterm (Compliance: 0.5 mL/cmH2O) and 3.5 kg term (1.0 mL/cmH2O) model.Results2298 breaths were analysed (760 rPAP, 795 Neopuff constant VT, 743 Neopuff constant PRM). With CPAP at 9 cmH2O and set VT the mean ∆P (cmH2O) rPAP vs Neopuff 1.1 vs 5.6 (preterm) and 1.9 vs 13.4 (term), WOB (mJ) 4.6 vs 6.1 (preterm) and 35.3 vs 44.5 (term), and with set PRM mean VT (ml) decreased to 6.2 vs 5.2 (preterm) and 22.3 vs 17.5 (term) p<0.001. Similar results were found at pressures of 5 and 7 cmH2O.ConclusionrPAP had smaller pressure swings than Neopuff at all CPAP levels and was thus more pressure stable. WOB was higher with Neopuff when VT was held constant. VT reduced with Neopuff when respiratory effort was constant.
A Comparative Analysis of Primary and Bailout ADR in CTO‐PCI
Authors: Yang Lirui , Zhang Tao , Wang Gang , Ru Leisheng , Zhao Lin 
Published: November 2024

Objective: To compare the efficiency and safety of primary and bailout antegrade dissection and re‐entry (ADR) during percutaneous coronary intervention (PCI) for chronic total occluded (CTO) lesions.Methods: Patients who underwent ADR procedures for CTO lesions were enrolled in this study and were divided into two groups (primary ADR and bailout ADR) based on the initiation of ADR during PCI. The success rate and major adverse cardiovascular and cerebrovascular events (MACCE) during hospitalization were assessed as the primary endpoints. Procedure time, fluoroscopy time, wire crossing time, radiation dose, and contrast volume were considered as secondary endpoints.Results: A total of 243 patients were enrolled in this study, with 127 patients receiving primary ADR and 116 receiving bailout ADR. No differences were found in baseline parameters between the two groups. The success rate was significantly higher in the primary ADR group compared to the bailout ADR group (89.0% versus 77.6%, respectively; p = 0.017). The incidence of in‐hospital MACCE was low in both groups, with no significant differences observed between them. Procedure time, radiation dose, and contrast volume were lower in the primary ADR group compared to the bailout ADR group. Cox regression analysis revealed that primary ADR and J‐CTO score ≥ 3 were independent predictors of technical success in CTO‐PCI.Conclusions: Both primary and bailout ADR are safe strategies for CTO‐PCI. Primary ADR significantly improves the success rate of PCI for CTO compared to bailout ADR, with shorter operation time, lower radiation dose, and reduced contrast volume.
Manual Therapy Exerts Local Anti‐Inflammatory Effects Through Neutrophil Clearance
Authors: Liu Hongwen , Yuan Shiguo , Zheng Kai , Liu Gaofeng , Li Junhua , Ye Baofei , Wang Yangkun , Yin Li , Li Yikai 
Published: November 2024

Background: Manual therapy (MT) has been widely used in China to treat local tissue inflammation for a long time. However, there is a lack of scientific evidence for using MT in anti‐inflammatory therapy, and its anti‐inflammatory mechanism needs further clarification.Methods: We utilized MT to treat cardiotoxin (CTX) injury‐induced skeletal muscle inflammation in C57BL6/J mice. We analyzed the underlying mechanism by integrating single‐cell RNA sequencing (scRNA‐seq) with molecular techniques. Hematoxylin and eosin (H&E) and immunohistochemical (IHC) staining were used to assess skeletal muscle inflammation and muscle fiber cross‐sectional area (CSA). scRNA‐seq, immunofluorescence, and western blot were performed to determine cellular and molecular outcome changes.Results: Compared with CTX injury‐induced skeletal muscle inflammatory mice, MT intervention significantly reduced proinflammatory cytokines interleukin (IL)‐1β, IL‐6, and tumor necrosis factor alpha (TNF‐α) expression levels; scRNA‐seq detected that neutrophil numbers and activity were maximum proportions increased in injured skeletal muscle among macrophage, T cells, B cells, endothelial cells, fast muscle cells, fibroblasts, and skeletal muscle satellite cells; and S100A9 gene expression was supreme in neutrophils. However, after treatment with MT, S100A9 protein expression and the numbers and activity of Ly6g+/Mpo+ neutrophils were significantly inhibited, thus reducing the inflammatory cytokine levels and exerting an anti‐inflammatory effect by early clearing neutrophils.Conclusion: MT can mitigate localized inflammation induced by injured skeletal muscle, achieved by decreasing S100A9 protein expression and clearing neutrophils in mice, which may help advance therapeutic strategies for skeletal muscle localized inflammation.
Atrial Topology for a Unified Understanding of Typical and Atypical Flutter
Authors: Duytschaever Mattias , Van den Abeele Robin , Carlier Niels , Bezerra Arthur Santos , Verstraeten Bjorn , Lootens Sebastiaan , Desplenter Karel , Okenov Arstanbek , Nezlobinsky Timur , Shah Dipen , Haas Annika , Luik Armin , Martens Jordi , El Haddad Milad , De Smet Maarten , De Becker Benjamin , Francois Clara , Le Polain de Waroux Jean-Benoit , Tavernier Rene , Knecht Sebastien , Hendrickx Sander , Vandersickel Nele 
Published: November 2024

BACKGROUND:
Macroreentry stands as the predominant mechanism of typical and atypical flutter. Despite advances in mapping, many aspects of macroreentrant atrial tachycardia remain unsolved. In this translational study, we applied principles of topology to understand the activation patterns, entrainment characteristics, and ablation responses in a large clinical macroreentrant atrial tachycardia database.

METHODS:
Because the atrium can be topologically seen as a closed sphere with holes, we used a computational fixed spherical mesh model with a finite number of holes to induce and analyze macroreentrant atrial tachycardia. The ensuing insights were used to interpret high-density activation maps, postpacing interval-tachycardia cycle length values (difference between postpacing interval and tachycardia cycle length), and ablation response in 131 cases of typical and atypical flutter (n=106 left atrium, n=25 right atrium).

RESULTS:
Modeling of macroreentrant atrial tachycardia revealed that reentry on closed surfaces consistently manifests itself as paired rotation and that an odd number of critical boundaries is mathematically impossible. Together with mathematical confirmation by the index theorem, this led to a unifying construct that could explain the number of loops, difference between postpacing interval and tachycardia cycle length values, and ablation outcomes (termination, no change, or prolongation in tachycardia cycle length) in all 131 cases.

CONCLUSIONS:
Combining topology with the index theorem offers a novel and cohesive framework for understanding and managing typical and atypical flutter.
Virtual Reality for Preprocedure Planning of Covered Stent Correction of Superior Sinus Venosus Atrial Septal Defects
Authors: Stephenson Natasha , Rosenthal Eric , Jones Matthew , Deng Shujie , Wheeler Gavin , Pushparajah Kuberan , Schnabel Julia A. , Simpson John M. 
Published: November 2024

BACKGROUND:
Covered stent correction (CSC) of a superior sinus venosus atrial septal defect is an alternative to surgery in selected patients, but anatomic variation means that assessment for CSC requires a 3-dimensional anatomic understanding. Heart VR is a virtual reality (VR) system that rapidly displays and renders multimodality imaging without prior image segmentation. The aim of this study was to evaluate the performance of the Heart VR system to assess patient suitability for CSC.

METHODS:
In a blinded fashion, 2 interventionalists reviewed preprocedural computed tomography scans using Heart VR to assess the feasibility of CSC, including the potential need for pulmonary vein protection. The total review time using VR was recorded.

RESULTS:
Using conventional imaging, 15 patients were deemed suitable for CSC, but at catheterization, 3 cases were unsuitable. Using VR, when both interventionalists agreed that a case was suitable for CSC (n=12), all proved technically feasible. In the 3 cases that were unsuitable for CSC, the interventionalists using VR were either uncertain (n=1) or did not agree on suitability (n=2). The strategy for pulmonary vein protection was correctly identified by interventionalist 1 and 2 in 9/12 and 8/12 cases, respectively. In cases where pulmonary vein protection was required intraprocedurally (n=5), this was correctly identified using Heart VR. Using VR, in 3 cases it was determined that pulmonary vein protection would be required, but this was not the case on balloon interrogation. VR data loading and review times were 82 seconds and 7 minutes, respectively. Verbal feedback indicated that Heart VR assisted in the assessment of case suitability.

CONCLUSIONS:
Heart VR is a rapid and effective tool for predicting suitability for CSC in patients with a superior sinus venosus atrial septal defect and could be a feasible alternative to segmented virtual or physical 3-dimensional models.