bluuebunny/crossref_metadata_2025_split huggingface dataset:
A mode of CVVH with regional citrate anticoagulation compared to no anticoagulation for acute kidney injury patients at high risk of bleeding
Authors: Gao Jianping , Wang Feng , Wang Yonggang , Jin Dan , Tang Liping , Pan Konghan 
Published: April 2019

AbstractThe study was designed to assess a practical mode of postdilution continuous venovenous hemofiltration (CVVH) with regional citrate anticoagulation (RCA) using a calcium-containing replacement solution, and to compare it with a CVVH mode with no anticoagulation (NA). Both methods were employed in our center for acute kidney injury (AKI) patients at high risk of bleeding. Fifty-six patients were equally allocated into the RCA-CVVH group and the NA-CVVH group. The study displayed no significant differences between groups involving baseline characteristics, severity level, blood gas analysis, hepatic/renal/coagulative functions, electrolytes, hemoglobin concentration, and platelet counts before or after continuous renal replacement therapy (CRRT). Compared to the NA-CVVH group, the RCA-CVVH group had a lower level of transfused packed red blood cells and platelet as well as a longer filter lifespan. The result showed no substantial differences between groups in terms of the mean supporting time and cost involving CRRT per person, the length of ICU and hospital stays, and the ICU survival. Homeostasis was basically preserved at a target range during the RCA post-CVVH procedure. Serious complications did not arise during the RCA process. RCA postdilutional CVVH is a safe and effective mode for application in AKI patients with a high risk of bleeding, and it can extend the filter lifespan and decrease blood loss, compared with the NA mode for CRRT. Further studies are needed to evaluate this mode for CRRT. (Retrospective Registration number ChiCTR1800016462, Registration date 2/6/2018)
Decomposition and oxidation of methionine and tryptophan following irradiation with a nonequilibrium plasma jet and applications for killing cancer cells
Authors: Uchida Giichiro , Mino Yusuke , Suzuki Tensho , Ikeda Jun-ichiro , Suzuki Takashi , Takenaka Kosuke , Setsuhara Yuichi 
Published: April 2019

AbstractWe present evidence for the decomposition and oxidation of amino acids in aqueous solution following irradiation with a nonequilibrium plasma jet. Of 15 amino acids tested in cell culture medium, plasma irradiation induced a marked chemical change in methionine and tryptophan due to the effective production of reactive oxygen species by plasma-water interaction. We also report that plasma-treated methionine and tryptophan aqueous solutions can kill cancer cells, greatly decreasing the viability of human endometrial carcinoma (HEC-1) cancer cells due to the presence of decomposition or oxidation products generated from the amino acid. Plasma-treated methionine and tryptophan aqueous solutions also induced an anti-cancer effect on cancer-initiating cells.
Downregulation of CYB5D2 is associated with breast cancer progression
Authors: Ojo Diane , Rodriguez David , Wei Fengxiang , Bane Anita , Tang Damu 
Published: April 2019

AbstractWe report here that CYB5D2 is associated with tumor suppression function in breast cancer (BC). CYB5D2 expression was significantly reduced in tamoxifen resistant MCF7 cells and in MCF7 cell-derived xenografts treated with TAM. CYB5D2 overexpression induced apoptosis in MCF7 cells; CYB5D2 knockdown enhanced MCF7 cell proliferation. Using the TCGA and Curtis datasets within the Oncomine database, CYB5D2 mRNA expression was downregulated in primary BCs vs breast tissues and HER2-positive or triple negative BCs vs estrogen receptor (ER)-positive BCs. Using the TCGA and Metabric datasets (n = 817 and n = 2509) within cBioPortal, 660 and 4891 differentially expressed genes (DEGs) in relation to CYB5D2 were identified. These DEGs were enriched in pathways governing cell cycle progression, progesterone-derived oocyte maturation, oocyte-meiosis, estrogen-mediated S-phase entry, and DNA metabolism. CYB5D2 downregulation decreased overall survival (OS, p = 0.0408). A CYB5D2-derived 21-gene signature was constructed and robustly correlated with OS shortening (p = 5.72e-12), and independently predicted BC deaths (HR = 1.28; 95% CI 1.08–1.52; p = 0.004) once adjusting for known clinical factors. CYB5D2 reductions displayed relationship with mutations in PIK3CA, GATA3, MAP3K1, CDH1, TP53 and RB1. Impressively, 85% (560/659) of TP53 mutations occurred in the 21-gene signature-positive BC. Collectively, we provide the first evidence that CYB5D2 is a candidate tumor suppressor of BC.
Cortical circuit alterations precede motor impairments in Huntington’s disease mice
Authors: Burgold Johanna , Schulz-Trieglaff Elena Katharina , Voelkl Kerstin , Gutiérrez-Ángel Sara , Bader Jakob Maximilian , Hosp Fabian , Mann Matthias , Arzberger Thomas , Klein Rüdiger , Liebscher Sabine , Dudanova Irina 
Published: April 2019

AbstractHuntington’s disease (HD) is a devastating hereditary movement disorder, characterized by degeneration of neurons in the striatum and cortex. Studies in human patients and mouse HD models suggest that disturbances of neuronal function in the neocortex play an important role in disease onset and progression. However, the precise nature and time course of cortical alterations in HD have remained elusive. Here, we use chronicin vivotwo-photon calcium imaging to longitudinally monitor the activity of identified single neurons in layer 2/3 of the primary motor cortex in awake, behaving R6/2 transgenic HD mice and wildtype littermates. R6/2 mice show age-dependent changes in cortical network function, with an increase in activity that affects a large fraction of cells and occurs rather abruptly within one week, preceeding the onset of motor defects. Furthermore, quantitative proteomics demonstrate a pronounced downregulation of synaptic proteins in the cortex, and histological analyses in R6/2 mice and human HD autopsy cases reveal a reduction in perisomatic inhibitory synaptic contacts on layer 2/3 pyramidal cells. Taken together, our study provides a time-resolved description of cortical network dysfunction in behaving HD mice and points to disturbed excitation/inhibition balance as an important pathomechanism in HD.
Remote underwater video reveals higher fish diversity and abundance in seagrass meadows, and habitat differences in trophic interactions
Authors: Zarco-Perello Salvador , Enríquez Susana 
Published: April 2019

AbstractSeagrass meadows play a key ecological role as nursery and feeding grounds for multiple fish species. Underwater Visual Census (UVC) has been historically used as the non-extractive method to characterize seagrass fish communities, however, less intrusive methodologies such as Remote Underwater Video (RUV) are gaining interest and could be particularly useful for seagrass habitats, where juvenile fish camouflage among the vegetation and could easily hide or flee from divers. Here we compared the performance of UVC and RUV methodologies in assessing the fish communities of two seagrass meadows with low and high canopy density. We found that RUV detected more species and fish individuals than UVC, particularly on the habitat with higher seagrass density, which sheltered more juveniles, especially herbivorous, and adult piscivorous of commercial importance, evidencing significant differences in energy flow from macrophytes to predators between seagrass habitats, and also differences in the ecosystem services they can provide. Considering the ongoing worldwide degradation of seagrass ecosystems, our results strongly suggest that fish surveys using RUV in ecologic and fisheries programs would render more accurate information and would be more adequate to inform the conservation planning of seagrass meadows around the world.
The inflammation-reducing compatible solute ectoine does not impair the cytotoxic effect of ionizing radiation on head and neck cancer cells
Authors: Rieckmann Thorsten , Gatzemeier Fruzsina , Christiansen Sabrina , Rothkamm Kai , Münscher Adrian 
Published: April 2019

AbstractEctoine is a natural protectant expressed by halophile bacteria to resist challenges of their natural environments, such as drought, heat or high salt concentrations. As a compatible solute, ectoine does not interfere with the cell’s metabolism even at high molar concentrations. External application of ectoine results in surface hydration and membrane stabilization. It can reduce inflammation processes and was recently tested in a pilot study for the prevention and treatment of chemotherapy-induced oral mucositis. Oral mucositis is especially frequent and severe in patients with head and neck squamous cell carcinoma (HNSCC), who receive radiotherapy or chemoradiation. It is extremely painful, can limit nutritional intake and may necessitate treatment interruptions, which can critically compromise outcome. As it was recently reported that in vitro ectoine has the ability to protect DNA against ionizing irradiation, it was the aim of this study to test whether ectoine may protect HNSCC cells from radiotherapy. Using HNSCC cell lines and primary human fibroblasts, we can show that in living cells ectoine does not impair DNA damage induction and cytotoxicity through ionizing radiation. We therefore conclude that testing the ectopic application of ectoine for its ability to alleviate early radiotherapy/chemoradiation-induced side effects is safe and feasible.
Vines avoid coiling around neighbouring plants infested by polyphagous mites
Authors: Nakai Tomoya , Yano Shuichi 
Published: April 2019

AbstractVines that coil around plants heavily infested with ambulate polyphagous mites can be heavily damaged by the mites. To explore whether vines avoid mite-infested plants, we observed the coiling responses of morning glory (Ipomoea nil var. Heavenly Blue) vines and bush killer (Cayratia japonica (Thunb) Gagnep) tendrils around nearby kidney bean (Phaseolus vulgaris L.) plants that were either uninfested or heavily infested with the two-spotted spider mite (Tetranychus urticae Koch). The proportions of I. nil vines that coiled around spider mite-infested and uninfested bean plants did not differ significantly; however, no C. japonica tendril coiled around spider mite-infested plants. The proportion of such tendrils was thus significantly lower than that around uninfested plants. The ability of C. japonica tendrils to avoid spider mite-infested plants would prevent serious “contact infections” by mites. We further found that tendril avoidance seemed to be attributable to the mite webs that covered infested plants; neither spider mite-induced bean volatiles nor spider mite intrusion onto tendrils seemed to explain the avoidance.
The material properties of naked mole-rat hyaluronan
Authors: Kulaberoglu Yavuz , Bhushan Bharat , Hadi Fazal , Chakrabarti Sampurna , Khaled Walid T. , Rankin Kenneth S. , Smith Ewan St. John , Frankel Daniel 
Published: April 2019

AbstractHyaluronan (HA) is a key component of the extracellular matrix. Given the fundamental role of HA in the cancer resistance of the naked mole-rat (NMR), we undertook to explore the structural and soft matter properties of this species-specific variant, a necessary step for its development as a biomaterial. We examined HA extracted from NMR brain, lung, and skin, as well as that isolated from the medium of immortalised cells. In common with mouse HA, NMR HA forms a range of assemblies corresponding to a wide distribution of molecular weights. However, unique to the NMR, are highly folded structures, whose characteristic morphology is dependent on the tissue type. Skin HA forms tightly packed assemblies that have spring-like mechanical properties in addition to a strong affinity for water. Brain HA forms three dimensional folded structures similar to the macroscopic appearance of the gyri and sulci of the human brain. Lung HA forms an impenetrable mesh of interwoven folds in a morphology that can only be described as resembling a snowman. Unlike HA that is commercially available, NMR HA readily forms robust gels without the need for chemical cross-linking. NMR HA gels sharply transition from viscoelastic to elastic like properties upon dehydration or repeated loading. In addition, NMR HA can form ordered thin films with an underlying semi-crystalline structure. Given the role of HA in maintaining hydration in the skin it is plausible that the folded structures contribute to both the elasticity and youthfulness of NMR skin. It is also possible that such densely folded materials could present a considerable barrier to cell invasion throughout the tissues, a useful characteristic for a biomaterial.
Transformation from Quantum to Classical Mode: the Size Effect of Plasmon in 2D Atomic Cluster System
Authors: Wu Reng-lai , Quan Jun , Tian Chunhua , Sun Mengtao 
Published: April 2019

AbstractOn the basis of tight-binding approximation, the energy absorption of 2D atomic clusters is calculated by the linear response theory. Through the energy-absorption peaks in the presence of different external potentials, various types of plasmon modes are specified in clusters with dozens to hundreds atoms, such as transverse dipole plasmon, longitudinal dipole plasmon, transverse quadrupole plasmon, and longitudinal quadrupole plasmon. Moreover, the transformation of plasmon from quantum to classical mode is observed in clusters with different shape and different electron density. The particular transformation process demonstrate that: there are only a few modes of plasmon in clusters with few-atoms; as the number of atoms in cluster is increased, the number of plasmon modes increases, the gaps between plasmon frequencies become smaller, the quantum modes of plasmon gradually evolve into continuous modes, and the dispersion of quantum-mode plasmon gradually transforms into the one of classical 2D plasmon. Such process reveals the size effect of plasmon in 2D clusters, which can be explained by the fact that the energy levels near the Fermi energy are denser and more compact in larger-size clusters.
Multi-functional regulator MapZ controls both positioning and timing of FtsZ polymerization
Authors: Feng Zhang , Zhang Jiahai , Xu Da , Jiang Yong-Liang , Zhou Cong-Zhao , Chen Yuxing 
Published: April 2019

Abstract
The tubulin-like GTPase protein FtsZ, which forms a discontinuous cytokinetic ring at mid-cell, is a central player to recruit the division machinery to orchestrate cell division. To guarantee the production of two identical daughter cells, the assembly of FtsZ, namely Z-ring, and its precise positioning should be finely regulated. In Streptococcus pneumoniae, the positioning of Z-ring at the division site is mediated by a bitopic membrane protein MapZ (mid-cell-anchored protein Z) through direct interactions between the intracellular domain (termed MapZ-N (the intracellular domain of MapZ)) and FtsZ. Using nuclear magnetic resonance titration experiments, we clearly assigned the key residues involved in the interactions. In the presence of MapZ-N, FtsZ gains a shortened activation delay, a lower critical concentration for polymerization and a higher cooperativity towards GTP hydrolysis. On the other hand, MapZ-N antagonizes the lateral interactions of single-stranded filaments of FtsZ, thus slows down the formation of highly bundled FtsZ polymers and eventually maintains FtsZ at a dynamic state. Altogether, we conclude that MapZ is not only an accelerator to trigger the polymerization of FtsZ, but also a brake to tune the velocity to form the end-product, FtsZ bundles. These findings suggest that MapZ is a multi-functional regulator towards FtsZ that controls both the precise positioning and proper timing of FtsZ polymerization.
Association of single nucleotide polymorphisms in the RAB5B gene 3′UTR region with polycystic ovary syndrome in Chinese Han women
Authors: Yu Jia , Ding Caifei , Guan Siqi , Wang Chenye 
Published: April 2019

Abstract
Objective: Previous genome-wide sequencing revealed that Ras-related protein Rab-5B (RAB5B) is a susceptible target in patients with polycystic ovary syndrome (PCOS).
Methods: Direct sequencing was performed to analyze the RAB5B gene rs1045435, rs11550558, rs34962186, rs705700, rs58717357, rs11171718, rs60028217, rs772920 loci genotypes in 300 PCOS patients and 300 healthy controls. The plasma microRNA (miRNA)-24, miR-320 levels were measured by reverse transcription fluorescent quantitative PCR (RT-qPCR).
Results: The risk of PCOS in C allele carriers of RAB5B gene rs1045435 locus was 3.91 times higher than that of G allele. The risk of PCOS in rs11550558 locus G allele was 4.09 times higher than A allele. The risk of PCOS in rs705700 locus C allele was 1.66 times greater than T allele. The risk of PCOS in rs11171718 locus A allele carrier was 3.84 times higher than G allele. The rs11550558 SNP was associated with PCOS risk only in those with age ≥ 31.1 years. And RAB5B gene rs11550558, rs1045435, and rs11171718 SNPs were significantly associated with PCOS risk only in subjects with BMI ≥ 23.8 kg/m2. We also found that the RAB5B gene rs1045435 SNP was associated with plasma miR-24 levels. The RAB5B gene rs11550558, rs705700, rs11171718 SNPs were correlated with plasma miR-230 levels.
Conclusion: The single nucleotide polymorphisms of the rs1045435, rs11550558, rs705700, and rs11171718 loci of the RAB5B gene are associated with PCOS risk. The rs1045435 locus is likely an miR-24 binding site, while rs11550558, rs705700, and rs11171718 loci may be miR-320 binding sites.
New type II Cepheids from VVV data towards the Galactic center
Authors: Braga V. F. , Contreras Ramos R. , Minniti D. , Ferreira Lopes C. E. , Catelan M. , Minniti J. H. , Nikzat F. , Zoccali M. 
Published: April 2019

Context. The Galactic center (GC) is the densest region of the Milky Way. Variability surveys towards the GC potentially provide the largest number of variable stars per square degree within the Galaxy. However, high stellar density is also a drawback due to blending. Moreover, the GC is affected by extreme reddening, therefore near infrared observations are needed.
Aims. We plan to detect new variable stars towards the GC, focusing on type II Cepheids (T2Cs) which have the advantage of being brighter than RR Lyrae stars.
Methods. We perform parallel Lomb-Scargle and Generalized Lomb-Scargle periodogram analysis of the Ks-band time series of the VISTA variables in the Vía Láctea survey, to detect periodicities. We employ statistical parameters to clean our sample. We take account of periods, light amplitudes, distances, and proper motions to provide a classification of the candidate variables.
Results. We detected 1019 periodic variable stars, of which 164 are T2Cs, 210 are Miras and 3 are classical Cepheids. We also found the first anomalous Cepheid in this region. We compare their photometric properties with overlapping catalogs and discuss their properties on the color-magnitude and Bailey diagrams.
Conclusions. We present the most extensive catalog of T2Cs in the GC region to date. Offsets in E(J − Ks) and in the reddening law cause very large (∼1–2 kpc) uncertainties on distances in this region. We provide a catalog which will be the starting point for future spectroscopic surveys in the innermost regions of the Galaxy.
High-energy ion impacts into the sulfur-bearing ice surface of Europa: an atomistic study of chemical transformations
Authors: Anders Christian , Urbassek Herbert M. 
Published: April 2019

Context.The ice surface of Europa is unique due to its high concentration of sulfur compounds such as SO4, SO2, and H2S. Energetic ion impacts originating from the magnetosphere of Jupiter may alter the composition of the ice surface.Aims.We explore the chemical alteration of the surface due to a 20 MeV sulfur ion impact, for which the most pronounced effects are expected, and monitor the chemical transformations occurring inside the ice.Methods.Molecular dynamics simulations are used based on a reactive (REAX) potential, which allows for the molecular breakups and the ensuing reactions to be followed on an atomistic scale.Results.We observe dissociation of SO4and also a loss of SO2, while SO3is created; this is in qualitative agreement with laboratory experiments. Hydrolysis of water leads to abundant formation of H+, H3O+and OH−; in addition, we predict the presence of both sulfurous acid, H2SO3, and sulfuric acid, H2SO4, as well as traces of carbonic acid, H2CO3. The irradiation produces H2and O2, which are free to escape from the surface, in agreement with the tenuous Europa atmosphere detected.Conclusions.Since magnetospheric sulfur ions have a high mass and may possess large energies, they provide a unique source of high energy deposition in the ice surface of Europa leading to abundant radiolysis fragments and products. In addition, sulfur compounds existing in the ice are chemically transformed, for example, by sulfite formation.
Mechanistic Links between Non-Coding RNAs and Myeloid Cell Inflammation in Atherosclerosis
Authors: Paloschi Valentina , Winter Hanna , Viola Joana , Soehnlein Oliver , Maegdefessel Lars 
Published: April 2019

AbstractInflammation plays a pivotal role in the chronicity of atherosclerotic lesion development and progression. Myeloid cells are involved in all stages of atherosclerosis development: they contribute in early phases to endothelial dysfunction and create a pro-inflammatory environment responsible for disease progression. Numerous studies over the last decade have repeatedly provided evidence for the crucial importance for different classes of non-coding ribonucleic acids (RNAs) in regulating gene expression, as well as messenger RNA and protein stability. Functional studies using tools to either over-express or inhibit these non-coding RNAs showcased strong effects on tempering vascular inflammation and atherosclerosis progression. With this current review article, we want to discuss prominent examples of non-coding RNAs, being either produced by myeloid cells or affecting their recruitment and activity in the context of vascular inflammation, atherosclerosis and consequential diseases (such as myocardial infarction and stroke). All of the discussed transcripts were thoroughly studied in mechanistic explorations, indicating that they have the capability to modulate inflammatory cascades in the vasculature during disease exacerbation.