bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biological and cultural drivers of oral microbiota in Medieval and Post-Medieval London, UK
Authors: Farrer A. G. , Bekvalac J. , Redfern R. , Gully N. , Dobney K. , Cooper A. , Weyrich L. S. 
Published: June 2018

AbstractThe trillions of microorganisms that live in association with the human body (microbiota) are critical for human health and disease, but there is a limited understanding of how cultural and environmental factors shaped our microbiota diversity through time. However, biomolecular remnants of the human oral microbiota - recovered from the calcified dental plaque (calculus) of our long-dead ancestors - are providing a new means of exploring this key relationship of our evolutionary history. Here, we correlate extensive experimental, archaeological, and biological metadata with 128 ancient dental calculus specimens from Medieval and Post-Medieval London, UK (1066 – 1853 CE). We identify a significant association between microbiota and oral geography (i.e. tooth type and tooth surface), which has confounded ancient microbiota studies to date. By controlling for oral geography, however, we identify the first associations between ancient microbiota and cultural and environmental signatures. We find significant links between ancient British microbiota structure and health, including skeletal markers of stress that may reflect low socioeconomic status. Furthermore, this study provides baseline data to explore factors that drive microbiota differentiation within and between ancient populations and highlights the potential of ancient microbiota to infer detailed health and sociocultural information about the past.
The evolutionary potential of diet-dependent effects on lifespan and fecundity in a multi-parental population ofDrosophila melanogaster
Authors: Ng’oma Enoch , Fidelis Wilton , Middleton Kevin M. , King Elizabeth G. 
Published: June 2018

AbstractThe nutritional conditions experienced by a population play a major role in shaping trait evolution in many taxa. Constraints exerted by nutrient limitation or nutrient imbalance can influence the maximal value that fitness components such as reproduction and lifespan attains, and organisms may shift how resources are allocated to different structures and functions in response to changes in nutrition. Whether the phenotypic changes associated with changes in nutrition represent an adaptive response is largely unknown. Further, it is unclear whether the response of fitness components to diet even has the potential to evolve in most systems. In this study, we use an admixed multiparental population ofDrosophila melanogasterreared in three different diet conditions to estimate quantitative genetic parameters for lifespan and fecundity. We find significant genetic variation for both traits in our population and show that lifespan has moderate to high heritabilities within diets. Genetic correlations for lifespan between diets were significantly less than one, demonstrating a strong genotype by diet interaction. These findings demonstrate substantial standing genetic variation in our population that is comparable to natural populations and highlights the potential for adaptation to changing nutritional environments.
RNA-Seq analysis of compatible and incompatible styles of Pyrus species at the beginning of pollination
Authors: Guan Yaqin , Li Kun , Wang Yongzhang , Ma Chunhui 
Published: June 2018

AbstractIn Rosaceae, incompatible pollen can penetrate into the style during the gametophytic self-incompatibility response. It is therefore considered a stylar event rather than a stigmatic event. In this study, we explored the differences in gene expression between compatibility and incompatibility in the early stage of pollination. The self-compatible pear variety “Jinzhuili” is a naturally occurring bud mutant from “Yali”, a leading Chinese native cultivar exhibiting typical gametophytic self-incompatibility. We collected the styles of ‘Yali’ and ‘Jinzhuili’ at 0.5 and 2 h after self-pollination and then performed high-throughput sequencing. According to the pathway enrichment analysis of the differentially expressed genes, “plant-pathogen interaction” was the most represented pathway. Quantitative PCR was used to validate these differential genes. The expression levels of genes related to pollen growth and disease inhibition, such as LRR (LEUCINE-RICH REPEAT EXTENSIN), resistance, and defensin, differed significantly between compatible and incompatible pollination. Interestingly, at 0.5 h, most of these genes were upregulated in the compatible pollination system compared with the incompatible pollination system. Calcium ion transport, which requires ATPase, also demonstrated upregulated expression. In summary, the self-incompatibility reaction was initiated when the pollen came into contact with the stigma.
Pou5f3, SoxB1 and Nanog remodel chromatin on High Nucleosome Affinity Regions at Zygotic Genome Activation
Authors: Veil Marina , Yampolsky Lev , Grüning Björn , Onichtchouk Daria 
Published: June 2018

AbstractThe zebrafish embryo is mostly transcriptionally quiescent during the first 10 cell cycles, until the main wave of Zygotic Genome Activation (ZGA) occurs, accompanied by fast chromatin remodeling. At ZGA, homologs of mammalian stem cell transcription factors (TFs) Pou5f3, Nanog and Sox19b bind to thousands of developmental enhancers to initiate transcription. So far, how these TFs influence chromatin dynamics at ZGA has remained unresolved. To address this question, we analyzed nucleosome positions in wild-type and Maternal-Zygotic (MZ) mutants for pou5f3 and nanog by MNase-seq. We show that Nanog, Sox19b and Pou5f3 bind to the High Nucleosome Affinity Regions (HNARs). HNARs are spanning over 600 bp, featuring high in vivo and predicted in vitro nucleosome occupancy and high predicted propeller twist DNA shape value. We suggest a two-step nucleosome destabilization-depletion model, where the same intrinsic DNA properties of HNAR promote both high nucleosome occupancy and differential binding of TFs. In the first step, already prior to ZGA, Pou5f3 and Nanog destabilize nucleosomes on HNAR centers genome-wide. In the second step, post-ZGA, Nanog, Pou5f3 and SoxB1 maintain open chromatin state on the subset of HNARs, acting synergistically. Nanog binds to the HNAR center, while the Pou5f3 stabilizes the flanks. The HNAR model will provide a useful tool for genome regulatory studies in the variety of biological systems.
Targeting BMI1 and MCL1 for Lung Adenocarcinoma Treatment
Authors: Lin Erh-Hsuan , Hsu Chiung-Fang , Jan Yi-Hua , Lin Tsung-Hsien , Chang Hsiang-Yi , Cheng Chun-Ming , Hsu Hui-Jan , Chen Wei-Wei , Chen Bo-Hung , Tsai Hsing-Fang , Li Jung-Jung , Huang Chi-Ying , Chuang Shih-Hsien , Chang Jia-Ming , Hsiao Michael , Wu Cheng-Wen 
Published: June 2018

AbstractLung cancer is the leading cause of cancer-associated death worldwide. Early metastasis and the recurrence remain major challenges for lung cancer treatment in clinic. Targeting the cancer stemness could be a potential strategy to restrain tumor progression. In the current study, we found that in lung adenocarcinoma (LAC), BMI1 and MCL1 play crucial roles in invasion, chemo-resistance, and tumor initiation. JNK signaling is a link between oncogenic pathway or environment stress to cancer stemness. The activation of JNK, either by EGFR or chemotherapy agent, stabilized BMI1 and MCL1 proteins through suppressing the expression of E3-ubiquitin ligase HUWE1. In lung cancer patient samples, high level of BMI1 is correlated with poor survival, and the expression of BMI1 is positively correlated with MCL1. A novel small-molecule BI-44 was synthesized, which effectively suppressed BMI1/MCL1 expression and inhibited tumor formation and progression in preclinical models. Targeting BMI1/MCL1 provides the basis for a new therapeutic approach in the treatment of LAC.
A hyperactive variant of theEscherichia colianaerobic transcription factor FNR enhances ionizing radiation resistance
Authors: Bruckbauer Steven T. , Trimarco Joseph D. , Wood Elizabeth A. , Battista John R. , Cox Michael M. 
Published: June 2018

AbstractWe have previously generated four replicate populations of ionizing radiation (IR)- resistantEscherichia colithough directed evolution. Sequencing of isolates from these populations revealed that mutations affecting DNA repair (through DNA double-strand break repair and replication restart), ROS amelioration, and cell wall metabolism were prominent. Three mutations involved in DNA repair explained the IR resistance phenotype in one population, and similar DNA repair mutations were prominent in two others. The remaining population, IR-3-20, had no mutations in the key DNA repair proteins, suggesting that it had taken a different evolutionary path to IR resistance. Here, we present evidence that a variant of the anaerobic metabolism transcription factor FNR isolated from population IR-3-20 can play a role in IR resistance. An FNR variant is unique to IR-3-20 and suggests a role for altered global metabolism through the FNR regulon as a means for experimentally-evolved IR resistance.
A comparative study of the influence of political systems on the art markets of East Asia and China
Authors: Robertson Iain 
Published: June 2018

Purpose
The purpose of this paper is to define and characterise the precise nature of these cultural systems and their resulting impact on the respective art and artists of each territory, by ascertaining the impact on those systems of their respective government and governance.

Design/methodology/approach
This paper is based on three approaches to art market modelling. All three are based on political ideologies. The first, which typifies the art markets of Western Europe and the USA, is predicated on a Pluralist and Neo-Liberal ideology. The others correspond to the systems of government in China, Taiwan, South Korea and Japan.

Findings
It has been shown in this paper that political systems and their accompanying ideology, born of cultural preferences, have impacted on the art markets of China, Taiwan, South Korea and Japan. It has been demonstrated that all four markets are employing variants of the international norm.

Research limitations/implications
The art that is exported from East Asia will only be accepted by East Asian national markets when East Asian art markets exercise a majority influence on emerging and transitional markets. It is not the intention of this paper to pursue this thought beyond the possibility that it may occur.

Practical implications
The ineluctable conclusion is, therefore, that the global art market is moving towards a bipolar affair.

Social implications
This paper also suggests the disengagement of East Asian and Chinese “culture” and art from a global (western) norm and production and consumption of national culture in East Asia by East Asians.

Originality/value
The paper looks (for the first time) at the direct (and subliminal) influence of political systems on art markets and the consequential effects of political ideology on the art markets of East Asia and China. The paper arrives at a series of precise definitions for the way that these art markets operate.
Assessment on external corrosion rates for API pipeline steels exposed to acidic sand-clay soil
Authors: Quej-Ake L.M. , Contreras A. , Liu H.B , Alamilla J.L. , Sosa E. 
Published: June 2018

Purpose
The purpose of this paper is to study the susceptibility to corrosion processes of X60, X65 and X70 steels immersed in sand-clay soil with pH 3.0, using electrochemical techniques, scanning electron microscopy (SEM), energy dispersive spectroscopy and X-ray diffraction (XRD).

Design/methodology/approach
Natural acidic soil sample was collected as close as possible to buried pipes (1.2 m in depth) in a Right of Way from south of Mexico. Both steels and soil were characterized through SEM and XRD. Then, open circuit potential was recorded for all steels exposed to soil at different exposure times. Thus, the electrochemical impedance spectroscopy (EIS) was traced, and anodic polarization curves were obtained.

Findings
The steel corrosion processes started when the active sites were exposed to natural acidic soil. However, corrosion rates decreased for three steels as immersion time increased, obtaining the highest corrosion rate for X60 steel (0.46 mm/year for 5 h). This behavior could be attributed to corrosion products obtained at different exposure times. While, 5 h after removing corrosion products, X65 steel was more susceptible to corrosion (1.29 mm/year), which was corroborated with EIS analysis. Thus, corrosion products for the three steels exposed to natural acidic soil depended on different microstructures, percentage of pearlite and ferrite phases, in which different corrosion processes could occur. Therefore, the active sites for carbon steel surfaces could be passivated with corrosion products.

Practical implications
The paper identifies the any implication for the research.

Originality/value
Some anodic peaks could be caused by metallic dissolution and was recorded using high positive polarization (high field of perturbation). In addition, the inductive effects and diffusion process were interpreted at low frequency ranges using EIS. According to X-ray diffraction (XRD), acidic soil had Muscovite containing aluminum and iron phases that were able to generate hydrogen proton at the presence of water; it might be promoted at the beginning of deterioration on low carbon steels. Steel surface cleaning after removing corrosion products was considered to study the possible diffusion phenomena on damaged steel surfaces using EIS.
Determining how packaging and labeling of Requeijão cheese affects the purchase behavior of consumers of different age groups
Authors: Ribeiro Ana Paula Lima , Carneiro João de Deus Souza , De Melo Ramos Thaís , Patterson Laura , Pinto Sandra Maria 
Published: June 2018

Purpose
The buying process is affected by many aspects, in which consumer’s age is certainly an important feature to be considered. The purpose of this paper is to evaluate the influence of packaging and labeling of Requeijão cheese on the purchasing behavior of different age groups of consumers in order to obtain marketing information that aid in increasing sales of Requeijão cheese.

Design/methodology/approach
Three focus groups were created, each one composed of ten participants. The first group was aged 18-30 years old, the second group included people aged 31-50, and the third group participants were aged between 51 and 70 years old. Requeijão cheese labeling and packaging was presented to all groups to facilitate a discussion. A moderator asked participants in the three groups about the factors on the packaging and labeling of Requeijão cheese that influence their desire to purchase. Seven final themes were analyzed by thematic analysis: packaging, color and images on label, information, lid, price and brand.

Findings
The factors of greatest importance that influenced purchasing for the first and second group were glass packaging and color labels. Groups 2 and 3 preferred a packaging size of 250 g, an aluminum cap protected with a plastic cap, information, and affordable prices. Group 1 demonstrated high interest in 220 g packaging, metal lid and brands. Group 3 preferred plastic packaging. All groups were attracted by images on labels related to the product origin. The results from this study may be advantageous for creating marketing strategies to increase sales of Requeijão cheese and similar milk products that are sold in comparable ways.

Practical implications
Concept and attitudes toward packaging and labeling have changed in modern times. The present study demonstrates how consumers of different age groups have divergent opinions about packaging and labeling of Requeijão cheese, factors that have huge influences in the purchasing of this product. The results from this study will aid in creating marketing strategies to increase sales of Requeijão cheese and similar milk products that are sold in similar conditions. The findings will also assist manufactures in creating bonds with costumers once packaging and labeling might be produced in accordance with the preference of each age group of consumers, and consequently makes the visual aspects of packaging more attractive to them.

Originality/value
This study collected information about the most features in packaging and labeling of Requeijão cheese that attract consumers in the food buying process. There are many companies that sell Requeijão cheese. Then, it is necessary to understand the needs of consumers in relation to packaging and labeling of similar products, to make it more competitive in the market and to meet the expectations of consumers in order to create bond with them.
Polymicrobial multidrug-resistant bacteria isolated from street vended fresh fruit juices in Pakistan
Authors: Siddique Muhammad Hussnain , Qamar Muhammad Usman , Hayat Sumreen , Aslam Bilal , Nadeem Habibullah , Hussain Sabir , Saqalein Muhammad , Saeed Javeria , Muzammil Saima 
Published: June 2018

Purpose
The purpose of this paper is to evaluate the prevalence and antibiograms of bacteria isolated from various fresh fruit juices at a local market in Faisalabad.

Design/methodology/approach
Fresh fruit juice samples (n=125) were randomly collected using aseptic technique. Each sample (10 mL) was serially diluted with 90 mL of sterile peptone water, from 1×10−1 to 1×10−5. Each dilution was then used to inoculate nutrient agar by surface spread plating. Aerobic colony counts (ACCs) were determined by colony counting. The isolates were sub-cultured on blood and MacConkey agar. Preliminary identification was achieved on the basis of colony morphology and culture characteristic, and confirmed by API® 20E, 20NE, and API® Staph testing. Antimicrobial susceptibility testing was carried out using the Kirby-Bauer disk diffusion assay, per CLSI 2015 guidelines.

Findings
The mean ACC ranged from 2.0×106 CFU/mL to 4.93×106 CFU/mL, with the highest ACC determined for orange juice. Overall, 153 polymicrobial were identified in 125 samples; 103 of these were Gram-negative rods (GNR) and 28 were Gram-positive cocci (GPC). Escherichia coli (n=38), Klebsiella pneumoniae (n=32) and Pseudomonas aeruginosa (n=24) were the predominant GNR; Staphylococcus aureus (n=28) was the predominant GPC. Antibiogram analysis revealed that all GNR were resistant to ampicillin. However, most E. coli isolates were resistant to ceftazidime (72.4 percent of isolates), and ceftriaxone and cefepime (68.9 percent), while most K. pneumoniae isolates were resistant to cefepime (72 percent) and ceftriaxone (64 percent). All S. aureus isolates were resistant to penicillin, while most (64 percent) were resistant to piperacillin; the most effective drugs against bacteria were vancomycin and imipenem.

Practical implications
The findings suggest that the local government regulatory food and public health authorities should take immediate emergency measures. Appropriate surveillance studies and periodic monitoring of food items should be regularly performed to safeguard public health.

Originality/value
The current study revealed the prevalence of multidrug-resistant bacteria in freshly prepared fruit juices sold by local street vendors.
Crisis, sectoral and geographical factors: financial dynamics of Italian cooperatives
Authors: Fusco Floriana , Migliaccio Guido 
Published: June 2018

Purpose
The purpose of this paper is to analyze the financial structure of Italian cooperatives in the period before and during the crisis (2004-2013), in relation to two discriminating factors. At this end, it focuses on two research questions: What financial dynamics the Italian cooperatives have involved before, during and after the 2008 crisis, that is, in the decade 2004/2013? Are there statistically differences between business sectors and geographic area?

Design/methodology/approach
Secondary data on AIDA database have been used. The financial structure is assessed using two ratios: the financial leverage ratio and quick ratio. The final sample consists of 1,446 cooperatives. The trend and exploratory analysis, analysis of variance and Tukey-Kramer post-hoc test have been used.

Findings
The financial structure of cooperatives has not been substantially affected by the crisis in any geographic area and business sector, by virtue of resilience of their business model. Moreover, these two factors produce statistically significant differences in the financial structure of cooperatives.

Research limitations/implications
The study takes into account only the cooperatives that survived the crisis, so, presumably, the strongest. Moreover, another and more ratios should be considered at the end to have a more complete view on the financial dynamics.

Originality/value
The literature on resilience of cooperatives is still not very rich. Moreover, this work analyses and integrates aspects and approaches that are not usually considered together.
Muslim minority women in Western Thrace: any room for transformative learning?
Authors: Panitsides Eugenia , Kiouka Eirini Andromachi 
Published: June 2018

Purpose
The purpose of this paper is to gain meaningful insights in the learning background, experiences and potential of Muslim minority women in Western Thrace.

Design/methodology/approach
Qualitative data were obtained through 12 semi-structured interviews, which underwent a three-level qualitative analysis, following the “grounded theory” methodology.

Findings
It was depicted that Muslim minority women in Western Thrace are susceptible to patriarchal gender norms governed by stereotypes that restrict women to reproductive and caring roles and deprive them of the fundamental human right to education. Although the data suggest that learning in adulthood evidently bears some transformative dynamic, the limited adult learning experiences of some interviewees in this study are far from accounting for any substantial transformation at the personal or community level.

Research limitations/implications
Subjectivity, biased responses and a limited sample are among research limitations, impeding the generalization of the results and calling for further investigation.

Originality/value
The originality of the study stems from providing a difficult to reach sample of underprivileged women with the opportunity to express their views and perceptions as regards education and learning, drawing on the identification of specific areas for potential interventions in order to transform their lives and communities.