bluuebunny/crossref_metadata_2025_split huggingface dataset:
A scoping review of the epidemiology of systemic sclerosis and its organ manifestations: 2018–2024
Authors: Good Samuel D. , Lee Ju Young , Johnson Robert E. , Volkmann Elizabeth R. 
Published: October 2024

Purpose of review
Updates from large, observational cohorts and new statistical techniques have resulted in new data on the epidemiology of systemic sclerosis (SSc). This scoping review uses data from 2018 to 2024 to describe the current understanding of the epidemiology of SSc and several of its organ- manifestations.

Recent findings
Our review identified new estimates for the global incidence and prevalence of SSc (1.4–8.6 per 100 000 person-years and 17.6–18.9 per 100 000 individuals, respectively). Mortality rates remain high, though mortality at younger ages has decreased. interstitial lung disease and pulmonary arterial hypertension remain the most common causes of death for patients with SSc. Literature on gastrointestinal (GI) manifestations of SSc was scarce, and we identified significant heterogeneity in results. Furthermore, data on the epidemiology of racial, ethnic and sex-based disparities was lacking.

Summary
New techniques for the evaluation of the epidemiology of SSc highlight the high morbidity and mortality of SSc, and a growing prevalence rate compared with prior eras. Further research is needed to address notable heterogeneity in the reporting of epidemiological data and understudied disease manifestations, including GI disease and health disparities in disease outcomes.
Cast-OFF 2: One Week Versus 3–5 Weeks of Plaster Cast Immobilization for Non- or Minimally Displaced Distal Radius Fractures—A Stepped Wedge Cluster Randomized Controlled Trial
Authors: de Bruijn Marcel A. N. , Boersma Emily Z. , van Silfhout Lysanne , van Ginkel Laura A. , Tromp Tjarda N. , van de Krol Erik , Edwards Michael J. R. , Stirler Vincent M. A. , Hermans Erik ,   Cast-OFF 2 Study Group
Published: October 2024

OBJECTIVES:
Implementation of 1 week of cast immobilization followed by gradually increasing wrist mobilization for non- or minimally displaced distal radius fracture (DRF) and comparison of the functional outcomes and pain scores with the usual care (3–5 weeks of cast immobilization).

METHODS:

Design:
A randomized stepped wedge cluster design, prospective cohort.

Setting:
Academic and peripheral hospitals in levels 1, 2, and 3 trauma centers.

Patient Selection Criteria:
All patients between 18 and 85 years old with an isolated non- or minimally and nonreduced DRF were eligible for inclusion. Participating hospitals were randomized to transition from usual care (3–5 weeks of cast immobilization) to 1 week of cast immobilization, following the stepped wedge design.

Outcome Measures and Comparisons:
Patient characteristics, secondary dislocation, surgical treatment, visual analog scale, Patient Rated Wrist Evaluation (PRWE), Patient Reported Outcomes Measurement Information System Pain Interference, Pain Catastrophizing Scale 4, and patient satisfaction were compared between control and intervention group at weeks 1, 3–5, 6, months 3, 6, and 12. A difference around 11 points on the PRWE scale was considered clinically significant.

RESULTS:
Four hundred two patients were included (control n = 197 vs. intervention n = 205, 267/135, female/male). There were no differences in age (53.7 ± 18.6 vs. 53.3 ± 19.5, P = 0.27), sex (66% vs. 67% female, P = 0.44), dominant hand fractured (44% vs. 53%, P = 0.39), and type of fracture (39% vs. 41% extra-articular, P = 0.44). After 6 weeks, the PRWE score showed no clinically significant differences (−4.5 [confidence interval −12.9 to 4.02], P = 0.30). No significant differences were observed for function, pain scores, and patient satisfaction between groups (all P > 0.05). Furthermore, there was no significant difference in secondary dislocation rate (control 1.5% vs. intervention 1.0%, P = 0.32) and operation rate (control 1.5% vs. 1.5% intervention, P = 0.92).

CONCLUSIONS:
This study compared 1 week of cast immobilization followed by gradually increasing wrist mobilization to the usual care of 3–5 weeks for nonreduced DRF. No clinically significant differences in function, pain scores, patient satisfaction, secondary dislocation, and operations were observed. Therefore, 1 week of plaster immobilization can be safely recommended for the non- or minimally displaced and nonreduced DRF treatment.

LEVEL OF EVIDENCE:
Therapeutic Level II. See Instructions for Authors for a complete description of levels of evidence.
The need for biopsychosocial menopause care: a narrative review
Authors: Evans Subhadra , Van Niekerk Leesa , Orellana Liliana , O'Shea Melissa , Druitt Marilla I. , Jones Sara , Corrigan Sarah , Heggen Campbell , Arashiro Zuleika , Baber Rodney , Satyen Lata , Skvarc Dave , Mikocka-Walus Antonina 
Published: October 2024

Abstract

Importance and Objective
Menopause is a reproductive transition affecting half the world's population. Stigma and limited availability of evidence-based treatments that support biopsychosocial well-being mean that this life stage is often associated with challenging symptoms and reduced quality of life (QoL). The aim of this review was to examine the extent literature on psychological and mind-body interventions to manage perimenopausal and postmenopausal symptoms, and apply an interdisciplinary clinical, research, and cultural knowledge framework to guide recommendations for improving QoL and healthy aging in this population. We also aimed to understand the ideal delivery mode for such interventions.

Methods
Using Scopus, Medline, and PubMed, a review of systematic reviews, guidelines, and randomized controlled trials was undertaken to examine the use of psychological and mind-body interventions to improve menopause-related QoL and symptoms, including hot flushes, depression, anxiety, fatigue, pain, and sleep; conclusions were based on an analysis of this available evidence and linked to clinical and cultural considerations.

Discussion and Conclusion
Empirical support exists for a number of psychological and mind-body approaches to support QoL and symptoms during the menopause transition. The literature also identifies the need for menopause care that is culturally responsive.
An online multimodal model of menopause care is thus recommended, incorporating evidence-based treatments (eg, cognitive behavioral therapy, yoga, hypnosis) and treatment techniques (eg, mindfulness, and education including nutrition support), as well as First Nations wisdom. We also recommend financial well-being approaches to support people undergoing menopause. Integrated, multimodal approaches should be available online to remove time, location, and healthcare access barriers, and be designed with diverse consumers to ensure equity for those underserved due to region, LGBTIQ+ assigned female at birth status, and for multicultural and First Nations people.
Forensic Mental Health Nurses' Perceptions and Experiences of Trauma-Informed Care in a High-Secure Hospital
Authors: Roberts Casey , Luder Michael , McMullen Crystal , Cole Rosalind , Dignam Paul , Ward Nicole , Ireland Michael 
Published: October 2024

ABSTRACT

Background
Implementation of trauma-informed care (TIC) into forensic clinical practice may contribute to positive outcomes for both patients and staff.

Aim
The current study aimed to describe forensic mental health nurses' perceptions and experiences of using TIC within a high-secure forensic hospital.

Method
Twenty-seven forensic mental health nurses were interviewed regarding their experiences of using TIC within a high-secure hospital.

Results
Participants recognized the importance of understanding the past traumatic experiences of patients in their care but described a number of barriers to the implementation of TIC. A lack of organizational support, the negative impact of patient psychopathology on nurse well-being, and environmental factors were all perceived to negatively impact the successful delivery of TIC. Participants also discussed a widespread lack of knowledge and confidence in the application of TIC but expressed great desire for training to improve service delivery.

Implications for Clinical Forensic Nursing Practice
The application of TIC into daily clinical practice appears to be suboptimal. To support nurse and patient well-being and contribute to a highly skilled trauma-informed workforce, further training in this area is vital.

Conclusion
Training in TIC may contribute to enhanced patient outcomes and promote nurse well-being and should be supported organizationally with sufficient resources.
Efficacy and safety of stem cell therapy for fistula management: an overview of existing systematic reviews
Authors: Tripathi Tripti , Mohan Syam , Alfaifi Hassan A. , Farasani Abdullah , R Roopashree , Sharma Pawan , Sharma Abhishek , Koul Apurva , Prasad G. V. Siva , Rustagi Sarvesh , Anand Jigisha , Sah Sanjit , Gaidhane Shilpa , Bushi Ganesh , Jena Diptismita , Khatib Mahalaqua N. , Shabil Muhammed , Abdelwahab Siddig I. , Bhopte Kiran , Pant Manvi , Mehta Rachana , Pandey Sakshi , Brar Manvinder , Chilakam Nagavalli , Balaraman Ashok K. 
Published: October 2024

Background:
Fistulas, abnormal connections between two anatomical structures, significantly impact the quality of life and can result from a variety of causes, including congenital defects, inflammatory conditions, and surgical complications. Stem cell therapy has emerged as a promising alternative due to its potential for regenerative and immunomodulatory effects. This overview of systematic reviews aimed to assess the safety and efficacy of stem cell therapy in managing fistulas, drawing on the evidence available.

Methods:
This umbrella review was conducted following the Joanna Briggs Institute (JBI) methodology to assess the efficacy and safety of stem cell therapy for treating various types of fistulas. A comprehensive search was performed across multiple electronic databases including PubMed, Embase, Cochrane Register, and Web of Science up to 5 May 2024. Systematic reviews focusing on stem cell therapy for fistulas were included, with data extracted on study design, stem cell types, administration methods, and outcomes. The quality of the reviews was assessed using the AMSTAR 2 tool, and meta-analyses were conducted using R software version 4.3.

Results:
Nineteen systematic reviews were included in our umbrella review. The stem cell therapy demonstrated by significant improvements in clinical remission rates, with a relative risk (RR) of 1.299 (95% CI: 1.192–1.420). Stem cell therapy enhanced fistula closure rates, both short-term (RR=1.481; 95% CI: 1.036–2.116) and long-term (RR=1.422; 95% CI: 1.091–1.854). The safety analysis revealed no significant increase in the risk of adverse events with stem cell therapy, showing a pooled RR of 0.972 (95% CI: 0.739–1.278) for general adverse events and 1.136 (95% CI: 0.821–1.572) for serious adverse events, both of which indicate a safety profile comparable to control treatments. Re-epithelialization rates also improved (RR=1.44; 95% CI: 1.322–1.572).

Conclusion:
Stem cell therapy shows promise as an effective and safe treatment for fistulas, particularly in inducing remission and promoting closure of complex fistulas. The findings advocate for further high-quality research to confirm these benefits and potentially incorporate stem cell therapy into standard clinical practice for fistula management. Future studies should focus on long-term outcomes and refining stem cell treatment protocols to optimize therapeutic efficacy.
Angular Insertion Depth in Inner Ear Malformations, Relationship to Cochlear Size, and Implications for Electrode Selection
Authors: Tellioğlu Burçay , Sennaroğlu Levent 
Published: October 2024

Objective
The objectives were to determine the interrater agreement of the Skull AP X-ray in measuring angular insertion depth (AID), to provide descriptive information about the insertion depths of different electrodes used in inner ear malformations (IEMs), to investigate the effect of cochlear size and electrode length on AID, and to guide clinicians in electrode selection in IEMs.

Study Design
Retrospective case review.

Setting
Tertiary referral center.

Patients
A total of 198 IEMs (n = 169 patients) and 60 cochleae with normal anatomy (n = 60 patients) were selected from patients with severe mixed or sensorineural hearing loss who presented to our clinic and underwent cochlear implantation (CI) between January 2010 and December 2022.

Interventions
Three neurotologists independently measured AID on Skull AP X-rays. Basal turn length of the cochlea was measured in axial and coronal oblique reformatted sections on HRCT images.

Main Outcome Measures
Interrater reliability (ICC) of the AID measurements on Skull AP X-ray, determining the impact of cochlea size and electrode length on AID measurements.

Results
The interrater reliability (ICC) test showed a high level of consistency in measuring AID in the Skull AP X-ray (R = 0.906, p < 0.001). In the control group, a negative correlation was observed between the AID and the basal turn length of the cochlea, while a positive correlation was found between electrode length and AID (R = 0.947, p < 0.001).

Conclusions
The Skull AP X-ray appears to be a dependable tool for measuring AID. In cases of IEMs, it is important to select an electrode of appropriate length, considering the dimensions of the cochlea.
Grasping time – longevity of vascularized composite allografts
Authors: Messner Franka , Sardu Claudia , Petruzzo Palmina 
Published: October 2024

Purpose of review
Despite significant advancements in the field of vascularized composite allotransplantation, challenges, particularly regarding the long-term viability and functionality of vascularized composite allotransplantation (VCA) grafts, persist. This paper provides a review of the current literature on the longevity of VCA grafts, focusing on factors influencing graft survival, immunological considerations and clinical outcomes.

Recent findings
Longevity of VCA grafts is influenced by a variety of peri- and postoperative factors including cold ischemia time, human leukocyte antigen matching, environmental exposure, psychosocial factors, adherence, immunosuppression, and complications. Due to the limited number of VCA transplants performed and heterogenous reporting, direct correlation of single factors with VCA outcomes remains inconclusive. Indirect evidence, however, supports their importance. High immunosuppressive burden, frequent occurrence of acute and accumulating cases of chronic rejection remain a significant challenge of the field.

Summary
Insights gained from this review aim to inform clinical practice and guide future research endeavors with the goal of ameliorating outcomes after VCA transplantation and facilitate wider use of VCA grafts for restoration of tissue defects.
Self-Leadership and Associated Factors in Nursing Students
Authors: Akdeniz Canberk , Duygulu Sergül 
Published: October 2024

Background:
Nurse leaders face high pressure, but self-leadership can help them thrive. Nursing students, therefore, should develop this skill before graduation.

Purpose:
To investigate nursing students’ self-leadership perceptions and associated factors, and to reveal when and how self-leadership strategies are utilized.

Methods:
An explanatory sequential mixed-methods design with interpretative description; 943 nursing students were surveyed and 13 were selected for qualitative analysis. Descriptive data were collected, and self-leadership was assessed using the Revised Self-Leadership Questionnaire. Focus group interviews were analyzed through thematic and content analysis.

Results:
Students who were employed, were involved in student clubs or organizations, held managerial roles, and had career goals exhibited higher self-leadership perceptions. Qualitative findings revealed that staying in comfort zones, overthinking, and procrastination hinder self-leadership, while self-respect, self-awareness, and self-determination enhance it.

Conclusions:
Nurse educators should design interventions that enable students to take on planning roles, advance at their own pace, and engage in opportunities to develop their self-leadership skills.
Qualitative Exploration of Abdominal Transplant Surgeon Perception of Retirement
Authors: Chumdermpadetsuk Ritah R , Montalvan Adriana , Canizares Stalin , Hsiao Ling , Sullivan Amy , Rivera Belen , Eckhoff Devin E , Lee David D 
Published: October 2024

Background:
For many surgeons, retirement is an emotionally evocative subject, tied to a sense of loss. With minimal guidelines to facilitate a smooth transition, physicians tend to be inadequately prepared. There are few qualitative studies exploring surgeons’ perspectives and none focused on transplant surgeons, a population with arguably unique challenges. We set out to define an “ideal” retirement for transplant surgeons, and identify behavioral and cognitive patterns associated with optimism towards retirement.

Study Design:
We conducted 60-minute semi-structured interviews with 30 division chiefs of transplant surgery to explore their perceptions of retirement. Thematic analysis using a framework approach was performed to identify key themes.

Results:
The cohort was predominantly male (80.0%) and White (76.7%), with 24.8 years in practice on average (range 12-40 years). Participants expressed desires to retire at the peak of their career trajectory and maintain autonomy in this transition. However, when naming signs of impending retirement, they often cited indicators of burnout. Attributes separating those who were optimistic from others included 1) well-rounded sense of identity, 2) holistic attitude towards one’s health and well-being, 3) belief in the ability to exert autonomy over retirement process through long-range planning, 4) community centered mindset, and 5) multifaceted and evolving view of career identity.

Conclusion:
While surgeons aspire to “go out on top,” many envision working until they can no longer endure it. This discrepancy highlights the need for normalizing discussions around retirement. With their unique insights, we have the opportunity to develop supportive interventions, such that transplant surgeons retire in a manner which preserves dignity and celebrates their legacy.
DNA Hanger: Surface Free Single-Molecule Blotting Platform
Authors: Seol Jincheol , Kim Byungju , Yu Eui-Sang , Jeong Cherlhyun , Lee Jong-Bong 
Published: October 2024

ABSTRACTImmunoblotting typically requires protein immobilization onto a membrane surface for quantification. Particularly, in real-time single-molecule blotting, stringent surface passivation is essential to prevent false-positive signals from nonspecific binding. In this study, we introduce a novel surface-free single-molecule blotting platform, termed the DNA Hanger. This platform employs a 3D structure of a quartz slide. Modifiedλ-phage DNA molecules are randomly biotinylated along their bases and suspended between 4 μm-high thin barriers, positioned away from the quartz slide surface. A light sheet, produced within the 3D structure, illuminates fluorophore-conjugated proteins bound to the biotinylated DNA, enabling single-molecule detection. The DNA Hanger assay significantly reduces nonspecific binding and enhances sensitivity to sub-picomolar concentrations, suggesting that this platform provides a novel, surface-condition-independent, and real-time approach in a wide range of single-molecule blotting.
Dissecting the Heterogeneity and Tumor-Associated Dynamics of Human Liver Group I ILC via scRNA Sequencing Data
Authors: Zhang Yumo , He Jitian , Li Xue , Chen Huaiyong , Yang Zhouxin , Wang Youwei 
Published: October 2024

AbstractSingle-cell transcriptome analysis has made outstanding contributions to the identification of new cell lineages and the study of cancer immune microenvironment. Yet, the characterization of human liver ILC1s and their dynamic changes in the tumor microenvironment have not been thoroughly studied at this detailed level. Here, we performed an integrated analysis of mouse and human liver immune cells to identify human liver ILC1 based on identified mouse liver ILC1 and to verify its functional similarity. Additionally, our findings highlighted the different expression patterns of the EOMES gene in human versus mouse group I ILCs, suggesting its reduced regulatory significance in human liver NK cells and ILC1 compared to murine models. A unique subset ofCD127hiintermediate ILCs (intILCs) was identified, exhibiting traits of both human liver NK cells and ILC1. Single-cell sequencing data analysis and TCGA were utilized to characterize the distinct alterations in the genes and functions of NK cells, ILC1, and intILCs in human hepatocellular carcinoma. It was found that the dynamic changes of ILC1 and intILCs, along with some of their subpopulations, may be key factors in tumor progression. This study provided new insights into the identification of ILC1 in human liver and the immunologic changes and mechanism of group I ILCs in the tumor microenvironment, and these findings may be applicable to improving the diagnosis and treatment of hepatocellular carcinoma.Graphical Abstract
CIGB-300 reverses chemo-resistance in MDR1-transfected lung squamous cancer cells
Authors: Wang Meifeng , Tang Dongfang , Luo Xiaofang , Liu Wan , Xie Jiale , Yi Ying , Lan Yaqin , Li Wen , Perea Silvio E. , Dessie Wubliker , Perera Yasser , Qin Zuodong 
Published: October 2024

AbstractBackgroundInhibition of ABC transporter protein activity is considered to be the most effective method to reverse multidrug resistance (MDR). In this study, we evaluated the MDR reversal potential of CIGB-300, a potent CK2 kinase inhibitor.MethodsABCB1 overexpressing lung adenocarcinoma NCI-H226 cells were constructed using lentivirus, and the expression of ABCB1 gene and protein was detected by real-time fluorescence quantitative PCR and Western blotting. MTT assay was used to assess the cytotoxicity and MDR reversal effect of CIGB-300.The effect of CIGB-300 on ABCB1 expression was determined by Western Blotting. Cell surface expression and subcellular localization of ABCB1 were examined by Flow Cytometry and Immunofluorescence Staining. Rh123 efflux and accumulation were measured by Fluorescent Enzyme Labeler and Flow Cytometry.ResultsCIGB-300 significantly increased the sensitivity of drug-resistant cells overexpressing the ABCB1 drug efflux pump (NCI-H226-ABCB1), while it had no effect on their parental cell lines. At the same time, its mechanism of action may be related to the inhibition of ABCB1 expression, which was dose-dependent, Moreover, in addition, we demonstrated that CIGB-300 reduced the expression of NFKB and CDC37 proteins.ConclusionsOur study elucidated that CIGB-300 reverses ABCB1-mediated MDR by inhibiting ABCB1 protein expression or intracellular signaling and provides a potential therapeutic strategy to improve tumor chemosensitivity.