bluuebunny/crossref_metadata_2025_split huggingface dataset:
Simultaneous Detection of Yersinia Enterocolitica and Listeria Monocytogenes in Foodstuffs by Capillary Electrophoresis and Microchip Capillary Electrophoresis Laser-Induced Fluorescence Detector
Authors: Li Yongru , Su Hongwei , Lan Yajia 
Published: May 2018

Abstract
Background: Food safety is one of the most important public health problems in the world, and pathogenic bacterium is a major factor causing serious foodborne diseases. Objective: Two methods of duplex PCR combined with capillary electrophoresis laser-induced fluorescence detector (CE-LIF) and microchip capillary electrophoresis laser-induced fluorescence detector (MCE-LIF) have been developed for the simultaneous detection of Yersinia Enterocolitica and Listeria Monocytogenes in various foods. The specific conservative sequences of these two bacteria were amplified. Methods: After labelled with nucleic acid dye SYBR Gold and SYBR Orange, the PCR products were analyzed by CE-LIF and MCE-LIF, respectively. Under the optimal conditions, the detection of PCR products of the target bacteria was achieved in less than 15 min by CE-LIF and within 6 min by MCE-LIF. Results: The alignment analysis demonstrated that the PCR products had good agreement with the sequences published in GenBank. The CE-LIF method could detect 10 CFU/mL Y. enterocolitica and L. monocytogenes, and the MCE-LIF method could detect 100 CFU/mL Y. enterocolitica and L. monocytogenes. The intraday precisions of migration time and peak area of DNA markers and PCR products were in the range of 1.13 to 1.18% and 1.60 to 6.29%, respectively, for CE-LIF and 1.18 to 1.48% and 2.85 to 4.06%, respectively, for MCE-LIF. Conclusions: The proposed methods could be applied to target bacterial detection infood samples rapidly, sensitively, and specifically. Highlights: Two new methods based on CE and MCE have been developed for the simultaneous detection of Y. enterocolitica and L. monocytogenes in foodstuffs, and they can detect the bacteria directly without any enrichment because of their high sensitivity.
Performance Comparison Between Monolithic, Core-Shell, and Totally Porous Particulate Columns for Application in Greener and Faster Chromatography
Authors: Ibrahim Adel Ehab , Hashem Hisham , Saleh Hanaa , Elhenawee Magda 
Published: May 2018

Abstract
Background: The introduction of monolithic rods and core-shell particles as new morphologies of packing materials different from the conventional totally porous particles resulted in a leap forward for performance in LC. Meanwhile, environmental safety has become increasingly important in many areas, especially in industry and research laboratories. Objective: This study compared the efficiencies of commercially available columns of different lengths and diameters when greener chromatographic conditions were utilized. The main purpose of this study is to help practitioners select the most appropriate stationary phase for faster and greener analysis. Methods: The three types of stationary phases were compared in terms of separation efficiency, number of theoretical plates, peak shape, selectivity, resolution, analysis time, mobile phase consideration, and permeability using six drug molecules. Results: Results indicated that core-shell and monolithic stationary phases had superiority over the conventional totally porous particles in terms of efficiency and speed of analysis. Monolithic rods had lower column backpressure and higher permeability, so they are more suitable for higher mobile phase flow rates and viscosities. However, core-shell particles provided enhanced peak shapes and number of theoretical plates. Conclusions: The choice will depend on the main purpose of analysis and the composition of the mobile phase. Compromise must be made to obtain the best trade-off between separation efficiency and analysis speed. Highlights: This study is the first to consider green chromatography concepts for the selection of the best stationary phase of new morphologies.
Three-Dimensional Pathology Specimen Modeling Using “Structure-From-Motion” Photogrammetry: A Powerful New Tool for Surgical Pathology
Authors: Turchini John , Buckland Michael E. , Gill Anthony J. , Battye Shane 
Published: May 2018

Context.—Three-dimensional (3D) photogrammetry is a method of image-based modeling in which data points in digital images, taken from offset viewpoints, are analyzed to generate a 3D model. This modeling technique has been widely used in the context of geomorphology and artificial imagery, but has yet to be used within the realm of anatomic pathology.Objective.—To describe the application of a 3D photogrammetry system capable of producing high-quality 3D digital models and its uses in routine surgical pathology practice as well as medical education.Design.—We modeled specimens received in the 2 participating laboratories. The capture and photogrammetry process was automated using user control software, a digital single-lens reflex camera, and digital turntable, to generate a 3D model with the output in a PDF file.Results.—The entity demonstrated in each specimen was well demarcated and easily identified. Adjacent normal tissue could also be easily distinguished. Colors were preserved. The concave shapes of any cystic structures or normal convex rounded structures were discernable. Surgically important regions were identifiable.Conclusions.—Macroscopic 3D modeling of specimens can be achieved through Structure-From-Motion photogrammetry technology and can be applied quickly and easily in routine laboratory practice. There are numerous advantages to the use of 3D photogrammetry in pathology, including improved clinicopathologic correlation for the surgeon and enhanced medical education, revolutionizing the digital pathology museum with virtual reality environments and 3D-printing specimen models.
Human Epidermal Growth Factor Receptor 2 Testing in Breast Cancer: American Society of Clinical Oncology/College of American Pathologists Clinical Practice Guideline Focused Update
Authors: Wolff Antonio C. , Hammond M. Elizabeth Hale , Allison Kimberly H. , Harvey Brittany E. , Mangu Pamela B. , Bartlett John M.S. , Bilous Michael , Ellis Ian O. , Fitzgibbons Patrick , Hanna Wedad , Jenkins Robert B. , Press Michael F. , Spears Patricia A. , Vance Gail H. , Viale Giuseppe , McShane Lisa M. , Dowsett Mitchell 
Published: May 2018

Purpose.—To update key recommendations of the American Society of Clinical Oncology (ASCO)/College of American Pathologists (CAP) human epidermal growth factor receptor 2 (HER2) testing in breast cancer guideline.Methods.—Based on the signals approach, an Expert Panel reviewed published literature and research survey results on the observed frequency of less common in situ hybridization (ISH) patterns to update the recommendations.Recommendations.—Two recommendations addressed via correspondence in 2015 are included. First, immunohistochemistry (IHC) 2+ is defined as invasive breast cancer with weak to moderate complete membrane staining observed in &gt;10% of tumor cells. Second, if the initial HER2 test result in a core needle biopsy specimen of a primary breast cancer is negative, a new HER2 test may (not “must”) be ordered on the excision specimen based on specific clinical criteria. The HER2 testing algorithm for breast cancer is updated to address the recommended workup for less common clinical scenarios (approximately 5% of cases) observed when using a dual-probe ISH assay. These scenarios are described as ISH group 2 (HER2/chromosome enumeration probe 17 [CEP17] ratio ≥2.0; average HER2 copy number &lt;4.0 signals per cell), ISH group 3 (HER2/CEP17 ratio &lt;2.0; average HER2 copy number ≥6.0 signals per cell), and ISH group 4 (HER2/CEP17 ratio &lt;2.0; average HER2 copy number ≥4.0 and &lt;6.0 signals per cell). The diagnostic approach includes more rigorous interpretation criteria for ISH and requires concomitant IHC review for dual-probe ISH groups 2 to 4 to arrive at the most accurate HER2 status designation (positive or negative) based on combined interpretation of the ISH and IHC assays. The Expert Panel recommends that laboratories using single-probe ISH assays include concomitant IHC review as part of the interpretation of all single-probe ISH assay results.
Pemanfaatan ovitrap dalam pengukuran populasi Aedes sp. dan penentuan kondisi rumah
Authors: Hidayati Lisa , Hadi Upik Kesumawati , Soviana Susi 
Published: May 2018

<span lang="EN-US">The incidence of dengue hemorrhagic fever (DHF) is still a public health problem in Indonesia. Observations over a period of 20 to 25 years since the beginning of the discovering of the disease, has show the increase of the diseases incidence every five years. The purpose of this study are 1) study the diversity of <em>Aedes’</em>s mosquitoes in Sukabumi City, 2) measure the <em>Aedes </em>population based on the number of eggs and ovitrap index, and 3) to know the correlation between ovitrap index and house condition. <em>Aedes</em> eggs were collected from 14 villages in Sukabumi City that has the highest incidence rate, started from May 2015 until August 2015. Collecting eggs is done by setting a trap eggs (ovitrap) as many as 230 pieces in 115 homes (indoor and outdoor). The results showed that <em>Ae. aegypti </em>were found inside houses and <em>Ae. albopictus </em>were outside houses.<em> </em>The number of eggs collected from ovitrap inside the houses were three times more than those collected from outside. Ovitrap index inside houses was 60%, or 1.6 times more than the ovitrap index outside the houses (37%) in 14 villages in Sukabumi. Houses with poor ventilation and sanitation increased the risk 3.09 times of number of ovitrap index. The results of this study could be use as basic information for the communities to improved environment hygiene through reduced mosquito breeding sites, thus degraded the incidence of dengue</span>
Les déterminants et les conséquences de l’orientation entrepreneuriale : enseignements et voies futures de recherche basés sur une étude bibliométrique (2001-2016)
Authors: Chebbi Hela , Sellami Mohamed , Saidi Sana 
Published: May 2018

Le concept de l’orientation entrepreneuriale (OE) apparaît dans la littérature comme un construit scientifique rigoureux et solide à partir duquel a pu s’élaborer un corps de connaissances cumulées et stables (Rauch, Wiklund, Lumpkin et Frese, 2009). Depuis le début du xxie siècle, nous avons assisté à un développement exponentiel des publications sur le thème de l’OE. Une avalanche de travaux de recherche publiés dans des revues académiques et professionnelles a émergé dans divers contextes pour accompagner cet intérêt grandissant à ce sujet. Il convient donc de comprendre et structurer la littérature sur l’OE afin d’en tirer les principaux enseignements. C’est dans ce cadre que nous conduisons cette étude bibliométrique qui présente en revue et structure les recherches publiées sur l’OE entre 2001 et 2016. Elle vise à identifier les principales voies futures de recherches sur le sujet. Cet article met particulièrement l’accent sur les facteurs susceptibles de favoriser ou freiner l’OE ainsi que sur les principales conséquences de ce concept. Il apporte une réponse à la question suivante : quels sont les interactions/liens, entre les déterminants et les conséquences de l’OE, encore inexplorés dans la littérature ? Pour ce faire, 492 articles académiques publiés sont examinés moyennant deux méthodologies différentes. La première, quantitative basée sur des indicateurs univariés, permet de dresser le paysage des travaux sur le sujet (revues, méthodologies les plus utilisées, pays). La deuxième, qualitative basée sur une analyse thématique (logiciel Freeplane) permet d’identifier tous les déterminants et les conséquences de l’OE tels qu’étudiés dans les travaux conceptuels, qualitatifs et quantitatifs. Des enseignements ont été tirés autour des interactions entre les différents facteurs.
Applying fecal microbiota transplantation (FMT) to treat recurrent Clostridium difficile infections (rCDI) in children
Authors: Fareed Shaaz , Sarode Neha , Stewart Frank J. , Malik Aneeq , Laghaie Elham , Khizer Saadia , Yan Fengxia , Pratte Zoe , Lewis Jeffery , Immergluck Lilly Cheng 
Published: May 2018

Background
Fecal Microbiota Transplantation (FMT) is an innovative means of treating recurrent Clostridium difficile infection (rCDI), through restoration of gut floral balance. However, there is a lack of data concerning the efficacy of FMT and its impact on the gut microbiome among pediatric patients. This study analyzes clinical outcomes and microbial community composition among 15 pediatric patients treated for rCDI via FMT.

Methods
This is a prospective, observational, pilot study of 15 children ≤18 years, who presented for rCDI and who met inclusion criteria for FMT at a pediatric hospital and pediatric gastroenterology clinic. Past medical history and demographics were recorded at enrollment and subsequent follow-up. Specimens of the donors’ and the patients’ pre-FMT and post-FMT fecal specimen were collected and used to assess microbiome composition via 16S rRNA gene sequencing.

Results
FMT successfully prevented rCDI episodes for minimum of 3 months post-FMT in all patients, with no major adverse effects. Three patients reported continued GI bleeding; however, all three also had underlying Inflammatory Bowel Disease (IBD). Our analyses confirm a significant difference between pre-and post-FMT gut microbiome profiles (Shannon diversity index), whereas no significant difference was observed between post-FMT and donor microbiome profiles. At the phyla level, post-FMT profiles showed significantly increased levels of Bacteroidetes and significantly decreased levels of Proteobacteria. Subjects with underlying IBD showed no difference in their pre-and post-FMT profiles.

Conclusion
The low rate of recurrence or re-infection by C. difficile, coupled with minimal adverse effects post-FMT, suggests that FMT is a viable therapeutic means to treat pediatric rCDI. Post-FMT microbiomes are different from pre-FMT microbiomes, and similar to those of healthy donors, suggesting successful establishment of a healthier microbiome.
CRISPR/Cas9 Genome Editing Using Gold‐Nanoparticle‐Mediated Laserporation
Authors: Bošnjak Berislav , Permanyer Marc , Sethi Maya K. , Galla Melanie , Maetzig Tobias , Heinemann Dag , Willenzon Stefani , Förster Reinhold , Heisterkamp Alexander , Kalies Stefan 
Published: May 2018

AbstractEngineered nucleases hold large potential for future gene therapy applications. An obstacle hampering their applications are delivery methods bearing efficiency, throughput, and viability of target cells. How this limitation can be overcome via gold‐nanoparticle‐mediated (GNOME) laserporation is demonstrated. It employs a picosecond laser setup and 200 nm gold nanoparticles, and its full capacity with CRISPR/Cas9 delivery is demonstrated. 70 kDa dextrans are utilized to probe delivery in adherent SC1 cells. Afterward, GNOME laserporation is used for transfection of crRNA:tracrRNA targeting the mouse CCR7 (mCCR7) into SpCas9 (Streptococcus pyogenes Cas9) and mCCR7 co‐expressing SC1 cells. Finally, ribonucleoprotein particles consisting of mCCR7 crRNA:tracrRNA and SpCas9 endonuclease are transfected into SC1 cells not expressing SpCas9. Gene knockout efficiencies of up to 65% are detected in the GNOME laserporated cells. To validate the simplicity of the approach, the same treatment parameters are used to successfully knock out CXCR3 in 25% of GNOME laserporated activated mouse CD8+ T cells. In conclusion, this is the first demonstration of the unique combination of nanotechnology and laser irradiation for gene editing via engineered nucleases.
Graphene Oxide‐Template Controlled Cuboid‐Shaped High‐Capacity VS4 Nanoparticles as Anode for Sodium‐Ion Batteries
Authors: Wang Sizhe , Gong Feng , Yang Shize , Liao Jiaxuan , Wu Mengqiang , Xu Ziqiang , Chen Cheng , Yang Xiaofei , Zhao Feipeng , Wang Bin , Wang Yuesheng , Sun Xueliang 
Published: May 2018

AbstractRoom‐temperature sodium‐ion batteries have attracted great attentions for large‐scale energy storage applications in renewable energy. However, exploring suitable anode materials with high reversible capacity and cyclic stability is still a challenge. The VS4, with parallel quasi‐1D chains structure of V4+(S22−)2, which provides large interchain distance of 5.83 Å and high capacity, has showed great potential for sodium storage. Here, the uniform cuboid‐shaped VS4 nanoparticles are prepared as anode for sodium‐ion batteries by the controllable of graphene oxide (GO)‐template contents. It exhibits superb electrochemical performances of high‐specific charge capacity (≈580 mAh·g−1 at 0.1 A·g−1), long‐cycle‐life (≈98% retain at 0.5 A·g−1 after 300 cycles), and high rates (up to 20 A·g−1). In addition, electrolytes are optimized to understand the sodium storage mechanism. It is thus demonstrated that the findings have great potentials for the applications in high‐performance sodium‐ion batteries.
Nickel‐catalyzed C−H bond Alkoxylation of Amides with Alcohols
Authors: Rajesh Nimmakuri , Sundararaju Basker 
Published: May 2018

AbstractAn efficient Nickel‐catalyzed C−H bond alkoxylation of benzamides and olefinic carboxamides with alcohols were achieved for the first time using bidentate auxiliary. We demonstrated high‐functional group compatibility and tolerates wide‐range of alcohols.
Unusual Li‐Storage Behaviour of Two‐Dimensional ReS2 Single Crystals
Authors: Chaturvedi Apoorva , Hu Peng , Ray Ayan , Kloc Christian , Madhavi Srinivasan , Aravindan Vanchiappan 
Published: May 2018

AbstractUnusual Li‐storage properties are observed for two‐dimensional ReS2 compared to its analogue MoS2. First, we prepared ReS2 single crystals (99.9 % crystallinity) by chemical vapour transport with high yield and utmost purity. Li‐storage properties are assessed using a standard half‐cell assembly with metallic Li as the counter electrode. The highly crystalline ReS2 rendered excellent electrochemical characteristics with interesting features. This logically leads to further exploration of ReS2 towards Li‐storage. Indeed, ReS2 could act as efficient intercalation host for Li‐ions with lower redox potential (∼1.2 V vs. Li) and decent reversibility (∼0.4 mol Li). On the other hand, high reversibility is registered while deep discharging of the active material. This eventually obeys the perfect conversion pathway. Interestingly, electrochemically activated ReS2 also rendered good reversibility and eventually serves as an insertion host with a working potential of ∼2 V vs. Li.
Long-term prognostic impact of surgical complications in the German Rectal Cancer Trial CAO/ARO/AIO-94
Authors: Sprenger T , Beißbarth T , Sauer R , Tschmelitsch J , Fietkau R , Liersch T , Hohenberger W , Staib L , Gaedcke J , Raab H-R , Rödel C , Ghadimi M 
Published: May 2018

Abstract

Background
The influence of postoperative complications on survival in patients with locally advanced rectal cancer undergoing combined modality treatment is debatable. This study evaluated the impact of surgical complications on oncological outcomes in patients with locally advanced rectal cancer treated within the randomized CAO/ARO/AIO-94 (Working Group of Surgical Oncology/Working Group of Radiation Oncology/Working Group of Medical Oncology of the Germany Cancer Society) trial.

Methods
Patients were assigned randomly to either preoperative chemoradiotherapy (CRT) followed by total mesorectal excision (TME) or postoperative CRT between 1995 and 2002. Anastomotic leakage and wound healing disorders were evaluated prospectively, and their associations with overall survival, and distant metastasis and local recurrence rates after a long-term follow-up of more than 10 years were determined. Medical complications (such as cardiopulmonary events) were not analysed in this study.

Results
A total of 799 patients were included in the analysis. Patients who had anterior or intersphincteric resection had better 10-year overall survival than those treated with abdominoperineal resection (63·1 versus 51·3 per cent; P &lt; 0·001). Anastomotic leakage was associated with worse 10-year overall survival (51 versus 65·2 per cent; P = 0·020). Overall survival was reduced in patients with impaired wound healing (45·7 versus 62·2 per cent; P = 0·009). At 10 years after treatment, patients developing any surgical complication (anastomotic leakage and/or wound healing disorder) had impaired overall survival (46·6 versus 63·8 per cent; P &lt; 0·001), a lower distant metastasis-free survival rate (63·2 versus 72·0 per cent; P = 0·030) and more local recurrences (15·5 versus 6·4 per cent; P &lt; 0·001). In a multivariable Cox regression model, lymph node metastases (P &lt; 0·001) and surgical complications (P = 0·008) were the only independent predictors of reduced overall survival.

Conclusion
Surgical complications were associated with adverse oncological outcomes in this trial.
Empathic behavior according to the state of others in mice
Authors: Ueno Hiroshi , Suemitsu Shunsuke , Murakami Shinji , Kitamura Naoya , Wani Kenta , Okamoto Motoi , Matsumoto Yosuke , Aoki Shozo , Ishihara Takeshi 
Published: May 2018

AbstractIntroductionEmpathic behavior is essential for social activities in social animals. Therefore, lack of empathy is a feature of several neuropsychiatric disorders. However, the underlying mechanisms of empathy and which animals possess it remain unclear. In this study, we investigated whether mice show empathic behavior.MethodsWe tested mice for empathy‐like behaviors toward conspecifics who were distressed. We investigated behavioral changes in cage‐mate or stranger mice.ResultsWhen the conspecific mice were in a painful state, subject mice showed preferential approach behavior toward them, presumably recognizing the state. Both visual information and olfactory information are indispensable for this empathic behavior.ConclusionsThese results suggest that the mouse recognizes the emotional state of the conspecific and engages in social interaction. The results of this study are useful for the elucidation of the causal mechanisms involved in neuropsychiatric disorders and may contribute in the development of novel treatment targets.
A prenatal case of partial trisomy 21 (q22.2q22.3), resulting from a paternal insertion translocation ins(16;21) and uncovered by QF‐PCR, and characterized by array CGH and FISH
Authors: Bhola Shama L. , Nieuwint Aggie W. M. , Stuurman Kyra E. 
Published: May 2018

Key Clinical MessageIn addition to detecting trisomies of whole chromosomes, QF‐PCR can also detect partial trisomies of the chromosomes 13, 18, and 21, which can suggest an unbalanced translocation. Additional testing with other techniques, such as microarray or FISH, is recommended when an unbalanced translocation is suspected.
Xanthogranulomatous pyelonephritis with psoas abscess and renocolic fistula
Authors: Kato Kazuya , Iwasaki Yoshiaki , Kato Yurina , Kato Kimitaka , Matsuda Minoru 
Published: May 2018

Key Clinical MessageXanthogranulomatous pyelonephritis (XGP) is an uncommon inflammatory disease of the kidney . Diffuse XGP is a rare condition which may spread into the pelvic cavity leading to fatal complications from a psoas muscle abscess and/or renocolic fistula . In diffuse type, nephrectomy and excision of the fistula is the recommended treatment.
Appendix epidermoid cyst: Presenting as an acute appendicitis
Authors: Rahbar Mahtab 
Published: May 2018

Key Clinical MessageWe report a rare case of a cystic mass in an appendix in a patient who presented nausea, vomiting, and sharp pain in lower right abdomen and mimicking acute appendicitis. Although this entity is very rare, careful physical observation, imaging, and pathology can be helpful to make an accurate diagnosis.