bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mechanism of zju‐miR156c‐mediated network in regulating witches' broom symptom of Chinese jujube
Authors: Wang Yunjie , Luo Zhi , Zhao Xuan , Sun Hongqiang , Liu Jiaxin , Zhang Dongfeng , Cao Haonan , Ai Changfeng , Wang Lihu , Dai Li , Liu Mengjun , Wang Lixin , Liu Zhiguo 
Published: November 2024

AbstractJujube witches' broom, caused by phytoplasma, is a destructive disease of Chinese jujube. Studies have shown that zju‐miR156s play an important role in phytoplasma infection in jujube, but the regulatory mechanism between zju‐miR156c and witches' broom remains unexplored. In the current study, miRNA‐seq and gene expression analysis showed that zju‐miR156c was more highly induced in infected jujube plants than the other miRNAs and its target gene was ZjSPL3. In addition, the expression levels of thymidylate kinase gene (TMKJWB) and secreted jujube protein (SJP1JWB) in diseased materials were higher than those in healthy controls. The expression level of zju‐miR156c was significantly upregulated, while ZjSPL3 was sharply downregulated and the content of cytokinin (CTK) significantly increased. Overexpression of zju‐miR156c in Arabidopsis significantly reduced the expression of AtSPL10 (homologous gene of ZjSPL3) but increased the content of CTK, and the transgenic plants exhibited witches' broom symptoms. In addition, yeast two‐hybrid and co‐immunoprecipitation assays confirmed that SJP1JWB interacted with ZjERF18. Yeast one‐hybrid analysis showed that ZjERF18 could interact with the promoter of zju‐MIR156c. In conclusion, our results demonstrated a novel pathogenic module of ZjERF18‐zju‐miR156c‐ZjSPL3‐CTK has an important function in the formation of witches' broom caused by SJP1JWB.
Development of COVID‐19 Infection Prevention and Control Training Program Based on ADDIE Model for Clinical Nurses: A Pretest–Posttest Study
Authors: Liang Bing , Zhang Jiaxin , Qu Zhifei , Jiang Nan , Chen Chen , Cheng Siming , Li Li 
Published: November 2024

ABSTRACTScientific and effective training is important to enhance the theoretical knowledge and practical skills of clinical nurses in coronavirus disease 2019 (COVID‐19) prevention and control (IPC), so as to improve the IPC capacity. The study aimed to design and implement a COVID‐19 IPC training program for clinical nurses based on the ADDIE model, and to evaluate its clinical application effects. This was a pretest–posttest study in which 389 clinical nurses were recruited through convenience sampling. The IPC training program for clinical nurses was developed based on the five stages of ADDIE model. Data were analyzed using pared‐sample t test, McNemar's test, and Wilcoxon test. After training, nurses' theoretical scores and operational scores except for hand hygiene were improved significantly (p < 0.001). Compared to pre‐training assessment, their job performances were also significantly improved (p < 0.001). And they had higher satisfaction with the program post‐training. Under the situation of COVID‐19, training based on the ADDIE model can effectively improve the IPC ability of clinical nurses. Besides, it provides new ideas, methods, and approaches for future clinical IPC training.
Health Professionals on Cross‐Sectoral Collaboration Between Mental Health Hospitals and Municipalities: A Critical Discourse Analysis
Authors: Jørgensen Kim , Jørgensen Kristine Bro , Frederiksen Jesper , Watson Emma , Hansen Morten , Karlsson Bengt 
Published: November 2024

ABSTRACTThis study investigates the role of language in cross‐sector collaboration between mental health hospitals and municipalities, focusing on the challenges of maintaining continuity of care and integrating patient‐centered approaches. Using Fairclough's framework for critical discourse analysis, we examined focus group interviews with 21 healthcare professionals, including nurses, social workers, and psychiatrists, to identify key themes and patterns in how cross‐sector collaboration is discussed. The analysis revealed a dominant medicalized discourse in hospital settings, which often emphasized structured care processes like treatment plans and medication management, overshadowing more flexible, patient‐centered approaches common in community‐based services. Power dynamics were evident, with hospital professionals frequently positioned as active agents, while patients and community‐based workers were portrayed in more passive roles. Although efforts to involve patients in decision‐making were noted, these were often controlled by professionals, reflecting a mediated approach to patient empowerment. The findings highlight the cultural and structural divides between hospital and community services and suggest the need for improved communication strategies, integrated care pathways, and a shift toward more inclusive, patient‐centered care models. Addressing these discursive barriers is crucial for achieving more effective, integrated, and patient‐centered care, ultimately improving outcomes for patients.
Is Generative AI Increasing the Risk for Technology‐Mediated Trauma Among Vulnerable Populations?
Authors: Abdulai Abdul‐Fatawu 
Published: November 2024

ABSTRACTThe proliferation of Generative Artificial Intelligence (Generative AI) has led to an increased reliance on AI‐generated content for designing and deploying digital health interventions. While generative AI has the potential to facilitate and automate healthcare, there are concerns that AI‐generated content and AI‐generated health advice could trigger, perpetuate, or exacerbate prior traumatic experiences among vulnerable populations. In this discussion article, I examined how generative‐AI‐powered digital health interventions could trigger, perpetuate, or exacerbate emotional trauma among vulnerable populations who rely on digital health interventions as complementary or alternative sources of seeking health services or information. I then proposed actionable strategies for mitigating AI‐generated trauma in the context of digital health interventions. The arguments raised in this article are expected to shift the focus of AI practitioners against prioritizing dominant narratives in AI algorithms into seriously considering the needs of vulnerable minority groups who are at the greatest risk for trauma but are often invisible in AI data sets, AI algorithms, and their resultant technologies.
AAAAI–EAACI PRACTALL: Standardizing oral food challenges—2024 Update
Authors: Sampson Hugh A. , Arasi Stefania , Bahnson Henry T. , Ballmer‐Weber Barbara , Beyer Kirsten , Bindslev‐Jensen Carsten , Bird J. Andrew , Blumchen Katarina , Davis Carla , Ebisawa Motohiro , Nowak‐Wegrzyn Anna , Patel Nandinee , Peters Rachel L. , Sicherer Scott , Spergel Jonathan , Turner Paul J. , Yanagida Noriyuki , Eigenmann Philippe A. 
Published: November 2024

AbstractThis common statement of the American Academy of Allergy, Asthma and Immunology (AAAAI) and The European Academy of Allergy and Clinical Immunology (EAACI) provides an update of the 2012 published guidelines on food challenges. The guidelines equally address food challenges in the research and the clinical settings. They first address the diagnostic tests which can guide the decision to conduct a challenge. Safety of food challenges is prime, and the various procedures and safety issues as well as medications potentially involved in challenges are extensively discussed. Challenges are suggested to be conducted with semi‐logarithmic incremental doses based on the protein content, typically for IgE‐mediated food allergy with intervals of 20–30 min between doses. Specific protocols for other types of reactions such atopic dermatitis or gastrointestinal food allergy are detailed separately. Proper stopping criteria are essential in order to reduce the risk of false‐positive diagnoses, but also severe reactions. The guidelines recommend criteria based on “go on,” “stop,” or “observation.” These revised guidelines will clearly provide much needed guidance for food challenges in the research and clinical settings. They will continue to evolve with new diagnostic tests or new needs in the field of food allergy.
The influence of pain on community reintegration after spinal cord injury
Authors: Henderson Valerie , Mashola Mokgadi Kholofelo 
Published: November 2024

AbstractBackgroundCommunity reintegration is an important goal for people living with a spinal cord injury (SCI), and pain is suspected to limit reintegration due to its limitations in daily functioning, mood, and sleep.ObjectivesTo determine the influence of pain on community reintegration in manual wheelchair users with SCI.MethodsThe Reintegration to Normal Living Index was used to determine community reintegration, while the DN4 and the Wheelchair User's Shoulder Pain Index were used to determine the presence of neuropathic and shoulder pain respectively. Associations and differences between the pain variables and participants with and without pain were analyzed with Spearman correlations and Mann–Whitney U‐tests using SPSS v27 at 0.05 significance level and 95% confidence interval.ResultsOf the 122 participants, 85.2% reported current pain, with a 77.7% median for community reintegration. Neuropathic pain (53.3%) was more common and severe than nociceptive shoulder pain (14.8%). There was no significant difference in community reintegration between participants with and without pain, nor any correlation between the overall presence of pain and community reintegration. The severity of pain, particularly shoulder pain, was negatively associated with taking trips out of town (p < 0.01), and overall community reintegration (p < 0.05).ConclusionIt is not the mere presence of pain that influences community reintegration, but rather the severity and the location of pain. Shoulder care and pain management need to be included in the rehabilitation program, as these are important considerations when rehabilitating people with SCI back into their communities.
Decoding the Double Stress Puzzle: Investigating Nutrient Uptake Efficiency and Root Architecture in Soybean Under Heat‐ and Water‐Stresses
Authors: Maslard Corentin , Arkoun Mustapha , Leroy Fanny , Girodet Sylvie , Salon Christophe , Prudent Marion 
Published: November 2024

ABSTRACTIn the context of climate change, associated with increasingly frequent water deficits and heat waves, there is an urgent need to maintain the performance of soybean, a leading legume crop worldwide, before its yield declines. The objective of this study was to explore which plant traits improve soybean tolerance to heat and/or water stress, with a focus on traits involved in plant architecture and nutrient uptake. For this purpose, two soybean genotypes were grown under controlled conditions in a high‐throughput phenotyping platform where either optimal conditions, heat waves, water stress or both heat waves and water stresses were applied during the vegetative stage. By correlating architectural to functional traits, related to water, carbon allocation and nutrient absorption, we were able to explain the stress susceptibility level of the two genotypes. We have shown that water flow in the plant is central to the uptake and allocation of mineral elements in the plant, despite its modulation by stress and in a genotype‐dependent manner. This cross‐analysis of plant ecophysiology and plant nutrition under different stresses provides new information, especially on the importance of mineral elements in the different plant organs, and can inform future crop design, particularly under changing climatic conditions.
Decreasing Endomyocardial Biopsy Frequency in Pediatric Heart Transplantation Using A Rejection Risk Prediction Score—A Single Center Study
Authors: Tolani Drishti , Butts Ryan J. , Sutcliffe David L. , Power Alyssa 
Published: November 2024

ABSTRACTBackgroundRejection remains an important cause of morbidity and mortality after pediatric heart transplantation (HT). Endomyocardial biopsy (EMB) is the gold standard for rejection diagnosis, but it comes with procedural risk. The frequency of EMB varies significantly across centers. Since April 2018, our center's surveillance EMB schedule is based on a rejection risk prediction score employing age, pre‐HT diagnosis, and panel reactive antibodies (PRA). We aimed to evaluate outcomes in the 1st year post‐HT before and after risk score implementation.MethodsPatients who underwent HT at our center at ≤ 18 years of age from January 2015 to December 2020 were reviewed. The primary endpoint was rejection‐free survival at 1 year‐post‐ HT. Clinical characteristics were compared for patients transplanted in Era 1 (January 2015–April 2018) and Era 2 (April 2018–December 2020). Cumulative 1‐year survival free from rejection and from rejection with hemodynamic compromise (RHC) was compared between eras using Kaplan‐Meier survival analysis.Results115 patients underwent HT during our study period (52 in Era 1 and 63 in Era 2). There was an increase in VAD utilization between eras (19% in Era 1 vs. 40% in Era 2, p = 0.025), but otherwise no significant difference in demographic or clinical variables between the two eras. No statistically significant difference in freedom from rejection or freedom from RHC was identified between the two eras. There was a 60% reduction in the median number of EMB per patient in the first year post‐HT after employing the score (5 in Era 1 vs. 2 in Era 2, p < 0.001).ConclusionsAfter employing a rejection risk prediction score, our center decreased the frequency of EMB without worsening early post‐HT outcomes, thus establishing the clinical applicability of this tool.
Discordance Between Caregiver and Transplant Clinician Priorities Regarding Post‐Heart Transplant Education
Authors: Goldart Evan M. , McCormick Amanda D. , Lim Heang M. , Batazzi Adriana , Yu Sunkyung , Duimstra Ashley , Lowery Ray , Uzark Karen , Glenn Thomas , Cousino Melissa K. 
Published: November 2024

ABSTRACTBackgroundPatient and caregiver education following pediatric heart transplant (HTx) is a cornerstone of post‐HTx care. Despite the importance of post‐HTx education, there are no standardized guidelines for topics and content. Education practices vary across clinicians and centers. Patient and caregiver priorities regarding post‐HTx education are unknown. This national survey study aimed to characterize adolescent HTx recipient, caregiver, and clinician values specific to post‐HTx educational topics.MethodsA cross‐sectional, national electronic survey was performed. Eligible participants included pediatric HTx recipients between the ages of 13–18, adult caregivers of pediatric HTx recipients, and pediatric HTx clinicians. An investigator‐designed survey was developed to assess the importance of 20 educational topics. Average scores on educational topics were compared between HTx recipients/caregivers and HTx clinicians using two‐sample t‐tests.Results120 survey responses were included, with a majority completed by HTx caregivers (N = 73; 61%) and clinicians (N = 43; 36%). Of the 20 educational topics assessed, there was a significant difference (p < 0.05) between educational values of HTx recipients/caregivers compared to HTx clinicians for 40% (N = 8) of the topics. For each educational topic that revealed a significant difference, HTx recipients/caregivers consistently placed greater value on the educational topic compared to HTx clinicians.ConclusionsPriorities regarding post‐HTx education do not always align between HTx recipients/caregivers and HTx clinicians. Results suggest that HTx recipients/caregivers place higher value on educational topics regarding daily life after HTx compared to HTx clinicians. We hope these findings better inform the approach to post‐HTx education and care for future pediatric HTx patients.
Scenes From a Sociolegal Career: An Informal Memoir
Authors: Kagan Robert A. 
Published: November 2024

ABSTRACTThis memoir describes the 40‐year unfolding, project by project, of my sociolegal field research on legal and regulatory processes. It provides brief accounts of my interactions and interviews with regulatory officials and with businesspeople responsible for regulatory compliance. It also describes my ventures into the cross‐national comparison of legal and regulatory institutions and the political systems that shape and support them.
The Amazonian snakebite burden: Unveiling seasonal dynamics in a region with tenfold higher incidence compared to the Brazilian average
Authors: Rocha Jorge Emanuel Cordeiro , Gomides Samuel Campos 
Published: November 2024

AbstractBackgroundAccidents caused by snakes constitute a serious public health problem in Latin America and worldwide. The situation in the Brazilian Amazon region is neglected, resulting in the highest incidence of cases per capita in the country. Furthermore, the distance from urban areas makes it difficult for the population to access timely and effective medical care, including antivenom treatment. The Brazilian Amazon is characterised by high ethnic and cultural diversity, yet it lacks robust epidemiological information that would allow for the development of surveillance and prevention policies. This study aimed to assess the epidemiological profile of individuals affected by snakebite accidents from 2007 to 2021 in a poorly studied region of the Brazilian Amazon.MethodsTo assess the relationship between snakebite accidents, rainfall and river flood levels, cross‐correlation functions were employed. Data from the public healthcare system database was used for this analysis.ResultsThe results indicate that the study area experiences a snakebite rate 10 times greater than the Brazilian average and double that of other Amazonian regions. The most affected victims consist of adult males residing in rural areas, with snakebite accidents being more common during the rainy season. Viperid snakes were responsible for most bites, with the lower limbs being the most common location of injury. The data revealed a positive correlation between the number of snakebite accidents both rainfall and fluvial data.ConclusionsThe accident peak coincides with the Brazil nut harvest season, a highly significant forest product for the economy of rural communities. Our data reinforces the need for public policies focused on environmental education and prevention, such as the use of boots and leg guards. These preventive measures have the potential to reduce the number of snakebites accidents by approximately 85%.
Hemostatic function, immunomodulatory capacity, and effects of lipemia in cold‐stored whole blood
Authors: Tan Joanne C. G. , Aung Htet Htet , Marks Denese C. 
Published: November 2024

AbstractBackgroundWhole blood (WB) is increasingly being used for resuscitation of trauma patients. Although platelet‐, red blood cell (RBC)‐ and plasma‐specific parameters in cold‐stored WB are well characterized, there has been limited investigation of biological response modifiers (BRMs), which may induce adverse reactions in recipients. The aim of this study was to evaluate the quality and function of RBC, platelets, plasma proteins, and BRMs in cold‐stored WB during storage.MethodsWB (n = 24) was collected into collected into citrate‐phosphate‐dextrose (CPD) anticoagulant, held overnight, processed through a platelet‐sparing filter, and stored at 2–6°C for 21 days. RBC, platelet, coagulation factor quality and function, and BRM concentrations were measured throughout the duration of storage.ResultsWB was effectively leukoreduced, with 99.98% reduction in leukocyte count and 81% platelet count recovery following filtration. Five WB units were significantly lipemic, with a visible lipid layer appearing after being cold storage overnight. These were more turbid with higher hemolysis compared to non‐lipemic units (p = .023). Despite a decrease in platelet count during storage (p < .001), hemostatic function as measured by thromboelastography was maintained for at least 21 days (R time and maximum amplitude; both p < .001). There was a significant increase in PF4, CD62P, and RANTES during cold storage (all p < .001).DiscussionWB retains hemostatic potential for at least 21 days of cold storage, and with further development, may be suitable for transfusion in Australia. Before implementation in Australia, quality control measures for lipemia and hemolysis would need to be defined as part of our manufacturing processes.
Coagulation assay results at birth in preterm infants: A cohort study highlighting the relevance of local reference values for interpretation
Authors: Houben Nina , Fustolo‐Gunnink Suzanne , Caram‐Deelder Camila , Visser Remco , Bosma Madeleen , Fijnvandraat Karin , Eikenboom Jeroen , van der Bom Johanna , Lopriore Enrico 
Published: November 2024

AbstractBackground and ObjectivesRoutine coagulation screens at birth are still standard in some European neonatal intensive care units (NICUs), although interpretation of these results is complex in preterm infants. It is unclear to what extent local coagulation assay results agree with published reference ranges when using different analysers and reagents. We aimed to assess coagulation assay results on day 1 of life in very preterm infants admitted to the NICU.Materials and MethodsWe included all preterm infants born below 32 weeks gestational age (GA) admitted to the Leiden University Medical Center between 2004 and 2020 in whom coagulation assays (prothrombin time [PT] and activated partial thromboplastin time [APTT]) were obtained during the first 24 h of life. Infants either diagnosed with major intraventricular haemorrhage or who received plasma transfusion before coagulation assay were excluded. We assessed coagulation assay results and compared the results between <28 weeks (extremely preterm) and 28–32 weeks (very preterm) GA groups.ResultsCoagulation assays were obtained at birth in 144 infants (144/2577; 5.5%) of whom 104 fulfilled the inclusion criteria. We found similar median PT and APTT values for extremely and very preterm infants (PT: 18.1 vs. 18.7 s [p‐value = 0.400]; APTT: 44.2 vs. 47.7 s [p‐value = 0.252], respectively).ConclusionWe found similar coagulation assay results at birth for extremely and very preterm infants; however, results deviated considerably from some of the published reference ranges. This may be due to differences between analysers and reagents, underlining the need for reference ranges calibrated to the equipment used per NICU.
POTENTIAL FOR ACCELERATED REPLICATION OF FRAXINUS SOGDIANA USING IN VITRO CULTURE AND IDENTIFICATION OF A RELIC TREE USING DNA-BARCODING
Authors: Amangeldinova M.K. , Bekkuzhina S.S. , Zhumabay N.B. , Tussipkan D. , Manabayeva S.A. 
Published: November 2024

Four species of Fraxinus including F. angustifolia subsp. syriaca (Boiss.) Yalt., F. sogdiana Bunge, F. pennsylvanica Marshall and F. americana L. grow in Kazakhstan. The study aimed to determine the conditions for awakening endogenous dormancy in vitro of F. sogdiana. In the control group without vernalization, the sterile culture yield at room temperature was 30%. However, with cold treatment, this yield increased to 60%, which is twice as high. It was observed that on MS medium without the addition of hormones, 22 microshoots were obtained from unwounded seeds, whereas 39 microshoots were obtained from wounded seeds during microclonal propagation.Phylogenetic relationships and species identification were analyzed using three DNA barcoding markers, comparing F. sogdiana with species from the NCBI database. rbcL and ITS markers did not show satisfactory interspecific genetic variability among F. species. Notably, matK gene sequences effectively differentiated F. sogdiana from other species. These results provide fundamental information that is valuable for future biotechnological and molecular studies.