bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phylogenomics, biogeography, and clade diversification of Paris (Melanthiaceae)
Authors: Ji Yunheng , Yang Lifang , Chase Mark W. , Liu Changkun , Yang Zhenyan , Yang Jin , Yang Jun-Bo , Yi Ting-Shuang 
Published: August 2019

Abstract
Background

Paris (Melanthiaceae) is an economically important but taxonomically difficult genus, which is unique in angiosperms because some species have extremely large nuclear genomes. Phylogenetic relationships within Paris have long been controversial. Based on complete plastomes and nuclear ribosomal DNA (nrDNA) sequences, this study aims to reconstruct a robust phylogenetic tree and explore historical biogeography and clade diversification in the genus.

Results

All 29 species currently recognized in Paris were sampled. Complete plastomes and nrDNA sequences were generated by the genome skimming approach. Phylogenetic relationships were reconstructed using the maximum likelihood and Bayesian inference methods. Based on the phylogenetic framework and molecular dating, biogeographic scenarios and historical diversification of Paris were explored. Significant conflicts between plastid and nuclear datasets were identified, and the plastome tree is highly congruent with past interpretations of the morphology. Ancestral area reconstruction indicated that Paris may have originated in northeastern Asia and northern China, and has experienced multiple dispersal and vicariance events during its diversification. The rate of clade diversification has sharply accelerated since the late Miocene.

Conclusions

Our results provide important insights for clarifying some of the long-standing taxonomic debates in Paris. Cytonuclear discordance may have been caused by ancient and recent hybridizations in the genus. The climatic and geological changes since the late Miocene, such as the intensification of Asian monsoon and the rapid uplift of Qinghai-Tibet Plateau, as well as the climatic fluctuations during the Pleistocene, played essential roles in driving range expansion and radiative diversification in Paris. Our findings challenge the theoretical prediction that large genome sizes may limit speciation.
AMBN gene polymorphism Alters the Caries Susceptibility of Adolescents in South China
Authors: Wang Ketian , Pang Liangyue , Yin Luoping , Li Xia , Zhang Jianming , Cui Tianqiang , Tao Ye , Lin Huan Cai 
Published: August 2019

Abstract
The aim of this study was to identify genetic factors under additive and dominance models that contribute to caries susceptibility, and to investigate into the interactions between these genes. A cross-sectional study was conducted among 1055 adolescents in Foshan, South China. The International Caries Detection and Assessment System (ICDAS) was used to identify caries. Demographic and environmental variables were analyzed. Twenty-three single nucleotide polymorphisms (SNPs) in 14 genes were identified from saliva samples. Regression analysis was used for the evaluation of direct and epistasis of genes under the hypothesis of an additive model and using the minor allele as the reference allele. After the adjustment by environmental factors, the G allele in AMBN (rs13115627) was a protective factor for caries under both additive model (P=0.007; OR=0.728; 95% CI, 0.579-0.916) and dominance model (P=0.021; OR=0.728; 95% CI, 0.556-0.953). No interactions between selected genes met the Bonferroni correction significance cutoff for multiple testing. Our results suggests that gender, one-child family, Cariostat score and Plaque Index have independent protective effects on dental caries. The polymorphisms in AMBN (rs13115627), under both additive and dominance models alters caries susceptibility of Adolescents in South China. No epistatic effect has been found between selected genes.
Prevalence, infection intensity and associated risk factors of Intestinal Schistosomiasis among primary school Children in Lira District, Northern Uganda
Authors: Byagamy John Paul , Malinga Geoffrey Maxwell , Angwech Harriet , Opiro Robert , Echodu Richard , Odongo-Aginya Emmanuel 
Published: August 2019

Abstract
Objective: The aim of this cross-sectional study was to determine the prevalence, infection intensity and associated risk factors of intestinal schistosomiasis among primary school children in Lira district, Uganda. The study was conducted among 532 primary school pupils aged 6-16 years from eight randomly selected primary schools (March-May 2017). Stool samples were collected and examined for schistosomiasis using Odongo-Aginya method. Data on socio-demographic characteristics and risk factors were obtained using questionnaires.
Results: The overall prevalence of Schistosoma mansoni was 35.7% indicating a moderate infection. Both males and females were equally affected with S. mansoni . Ogur sub county had highest prevalence (42.1%) than others. Akangi and Akano primary schools both had highest (21.1%) prevalence compared to the others. Individuals aged 14-16 and 10-14 years had heavy infections (mean epg) of 600 and 565 eggs, respectively than those aged 6-9 years. With regard to risk factors, source of drinking water, sub-county location and primary school were associated with infection. Participants who fetch water from spring (85,44.7%) had higher infection than those who fetch from other sources. The study recommends provision of safe water, periodic treatment of school-aged children with praziquantel and public health education to reduce prevalence of S. mansoni .
A blended eHealth intervention for insomnia following acquired brain injury study protocol for a randomized controlled trial
Authors: Ford Marthe , Geurtsen Gert J. , Groet Erny , Bennekom Coen A.M. Van , Someren Eus J.W. Van 
Published: August 2019

Abstract
Background Up to a third of stroke patients and patients with traumatic brain injury suffer from insomnia, including problems to fall asleep or stay asleep at night. Insomnia may exacerbate other brain damage-related problems, for example regarding cognitive functioning and emotional well-being, may lead to poorer quality of life, and may complicate recovery processes. Cognitive behavioural therapy for insomnia, delivered face-to-face or online, is found to be effective in the general population. However, despite the high prevalence and serious consequences of insomnia following acquired brain injury, studies on the efficacy of face-to-face cognitive behavioural treatment in this population are scarce, and this applies even more for studies on online cognitive behavioural therapy. Therefore, this study aims to evaluate the efficacy of a newly developed guided online cognitive behavioural therapy for insomnia following acquired brain injury. Methods A multicenter randomized controlled trial will be conducted, in which 48 patients diagnosed with stroke or traumatic brain injury, and insomnia will be randomly allocated to the online cognitive behavioural therapy for insomnia treatment group or the treatment as usual group. The treatment consists of 6 online cognitive behavioural therapy sessions given on a weekly basis and personalized feedback after each session, combined with face-to-face sessions. Outcomes will be assessed at baseline, immediately after the intervention period and at 6 week follow up. The primary outcome is the insomnia severity assessed with the insomnia severity index. Secondary outcome measures include sleep quality, sleep features derived from the sleep diary, fatigue, anxiety and depression, subjective cognitive functioning and societal participation. Discussion This study will provide insight on the efficacy of online cognitive behavioural therapy for insomnia following stroke and traumatic brain injury. Trial Register Netherlands Trial Register, NTR7082, 12 March 2018
eNACT: A protocol for a randomised placebo-controlled trial investigating the efficacy and mechanisms of action of adjunctive N-acetylcysteine for first episode psychosis
Authors: Cotton Sue , Berk Michael , Watson Amity , Wood Stephen , Allott Kelly , Bartholomeusz Cali , Bortolasci Chiara , Walder Ken , O'Donoghue Brian , Dean Olivia , Chanen Andrew , Amminger Paul , McGorry Patrick , Ratheesh Aswin , Dodd Seetal 
Published: August 2019

Abstract
Background First episode psychosis (FEP) may lead to a progressive, potentially disabling and lifelong chronic illness; however, evidence suggests that the illness course can be improved if appropriate treatments are given at the early stages. Nonetheless, the efficacy of antipsychotic medications is suboptimal, particularly for negative and cognitive symptoms, and more efficacious and benign treatments are needed. Previous studies have shown the antioxidant amino acid N-acetyl cysteine (NAC) reduces negative symptoms and improves functioning in chronic schizophrenia and bipolar disorder. Research is scarce as to whether NAC is beneficial earlier in the course of illness. The primary aim of this study is to determine the efficacy of treatment with adjunctive NAC (2g/day for 26 weeks) compared with placebo to improve psychiatric symptoms in young people experiencing FEP. Secondary aims are to explore the neurobiological mechanisms underpinning NAC and how they relate to various clinical and functional outcomes at 26- and 52-week follow-up. Methods ENACT is a 26-week randomised controlled trial of adjunctive NAC versus placebo, with a 26-week non-treatment follow-up period, for FEP. We will be recruiting 162 young people aged 15-25 years who have recently presented to, and are being treated at, the Early Psychosis Prevention and Intervention Centre, Melbourne, Australia. The primary outcome is the Total Score on the Positive and Negative Syndrome Scale which will be administered at baseline, and weeks 4, 8, 12, 26 (primary endpoint), and 52 (end of study). Secondary outcomes include: symptomatology, functioning, quality of life, neurocognition, blood-derived measures of; inflammation, oxidative and nitrosative stress, and magnetic resonance spectroscopy measures of glutathione concentration. Discussion Targeted drug development for FEP to date has generally not involved the exploration of neuroprotective agents. This study has the potential to offer a new, safe, and efficacious treatment for people with FEP, leading to better treatment outcomes. Additionally, the neuroprotective dimension of this study may lead to a better long-term prognosis for people with FEP. It has the potential to uncover a novel treatment that targets the neurobiological mechanisms of FEP and, if successful, will be a major advance for psychiatry.
Enhancement of de novo sequencing, assembly and annotation of the Mongolian gerbil genome with transcriptome sequencing and assembly from several different tissues
Authors: Cheng Shifeng , Fu Yuan , Zhang Yaolei , Xian Wenfei , Wang Hongli , Grothe Benedikt , Lu Xin , Xu Xun , Klug Achim , McCullagh Elizabeth 
Published: August 2019

Abstract
BACKGROUND: The Mongolian gerbil (Meriones unguiculatus) has historically been used as a model organism for the auditory and visual systems, stroke/ischemia, epilepsy and aging related research since 1935 when laboratory gerbils were separated from their wild counterparts. In this study we report genome sequencing, assembly, and annotation further supported by transcriptome sequencing and assembly from 27 different tissues samples. RESULTS: The genome was assembled using Illumina HiSeq 2000 and resulted in a final genome size of 2.54 Gbp with contig and scaffold N50 values of 31.4 Kbp and 500.0 Kbp, respectively. Based on the k-mer estimated genome size of 2.48 Gbp, the assembly appears to be complete. The genome annotation was supported by transcriptome data that identified 31 769 (>2000bp) predicted protein-coding genes across 27 tissue samples. A BUSCO search of 3023 mammalian groups resulted in 86% of curated single copy orthologs present among predicted genes, indicating a high level of completeness of the genome. CONCLUSIONS: We report a de novo assembly of the Mongolian gerbil genome that was further enhanced by assembly of transcriptome data from several tissues. Sequencing of this genome increases the utility of the gerbil as a model organism, opening the availability of now widely used genetic tools.
Pancreatic neck thickness: A possible key to stump closure technique selection after distal pancreatectomy
Authors: Nikov Andrej , Záruba Pavel , Bělina František , Ryska Miroslav 
Published: August 2019

Abstract
Background

To date, no stump closure technique has been shown to be superior in lowering the risk of postoperative pancreatic fistula (POPF) after pancreatectomy. The aims our study were to investigate the possibility to influence POPF risk by selection of stump closure technique according to pancreatic parenchyma thickness and to establish a thickness cut-off value for selection of a hand-sewn and stapled closure technique.

Methods

A retrospective analysis of consecutive patients who underwent distal pancreatectomy at a single centre was performed. Anatomical determination of the transection site (pancreatic neck, body, or tail) was based on operative report and postoperative follow-up computed tomography (CT), with the thickness measured on the most recent CT image before surgery. Patients were classified by the thickness of the transection site, and sub-classified according to stump closure technique. POPF incidence, morbidity, mortality, and baseline-characteristics were investigated between groups.

Results

Among the 115 cases included in the analysis, the incidence of POPF was 33%, with no difference between stapled (29.9%) and hand-sewn (37.5%) closure techniques (p=0.426), regardless of transection site thickness. Among those with a transection site <13 mm, the incidence of POPF was 4.6% in the stapler subgroup versus 45.5% in the hand-sewn subgroup (p=0.0002). Among patients with a transection site ≥13 mm, the incidence of POPF was 75% in the stapler subgroup versus 30.8% in the hand-sewn subgroup (p=0.007).

Conclusions

Transection in the pancreatic neck, stapler closure of thin parenchyma, and hand-sewn closure of thick parenchyma were associated with significantly lower risk of POPF.
Can a community-led intervention offering social support and health education improve maternal health?: a repeated measures evaluation of the PACT project run in a socially deprived London borough at 6 month follow-up
Authors: Brown June S.L. , Luderowski Ana Lucia , Namusisi-Riley Josephine , Moore-Shelley Imogen , Bolton Matthew David , Bolton Derek 
Published: August 2019

Abstract
Background
Social adversity can significantly influence the wellbeing of mothers and their children. Maternal health may be improved through strengthened support networks and better health literacy. Population level health improvement requires the optimizing of the collaboration between statutory services (e.g. midwifery and health visiting services), civic organisations (e.g. churches, schools) as well as community groups and parents themselves. Key elements in improving community engagement are co-production and community control. This study evaluates a co-produced and community-led intervention developed for ante-natal and post-natal mothers, offering social support and health education. The intervention was based in a deprived south London borough.
Methods
A pre-post design was used to assess changes in the maternal health of mothers before and 6 months after attending a weekly intervention offering two key elements: social support for mothers and children (called Mumspace), and health education for mothers (called Parent University). Measures used included depression (PHQ-9), anxiety (GAD-7), health literacy (New Vital Signs), social capital (ASSIS) and a satisfaction measure.
Results
Sixty-one mothers were recruited at baseline and 58 mothers (95.1%) followed up after 6 months. The PACT intervention attracted some “difficult to engage” mothers, specifically Nigerian (26.2%) and Latin American (9.8%) mothers, the proportion of which was 5.8% and 3.6% above that in census records. Significant improvements were found on depression and anxiety for all the mothers, but particularly significant changes were found for those with more severe problems at baseline. There were also improvements in health literacy for those with poor literacy at baseline, overall social capital as well as 4 out of the 8 specific aspects. Satisfaction with the service was high.
Conclusions
This community-led social support and health education intervention has engaged “difficult to engage” mothers, as well as found very positive outcomes from a 6 month follow-up of mothers. Improvements were found on mental health, with particularly marked changes in depression and anxiety of mothers with more severe problems. Improvements in health literacy and social capital were also found. This is a promising intervention for the maternal mental health of mothers. A more rigorous controlled trial is recommended to further examine its effectiveness.
Modified Hardinge approach is an alternative to trochanteric flip osteotomy for Pipkin type IV femoral head fracture
Authors: Chen I-Jung , Chou Ying-Chao , Hsu Yung-Heng , Yu Yi-Hsun 
Published: August 2019

Abstract
Background
Pipkin type IV femoral head (FH) fractures generally have poor prognoses. Although several surgical approaches are used, the optimal procedure is still under debate. The purposes of this study were to compare two approaches, the modified Hardinge approach and trochanteric flip osteotomy, for the treatment of Pipkin type IV FH fractures.

Methods
This retrospective study included 20 patients who underwent surgical treatment for Pipkin type IV FH fractures between January 2011 and September 2017 at a level 1 trauma center. Thirteen were treated using the modified Hardinge approach (group A) and seven with trochanteric flip osteotomy (group B). All patients completed at least 1 year of follow-up. The clinical outcome of the Merle d’Aubigné-Postel score; and radiological outcomes including the quality of the fracture reduction, the osteonecrosis of the FH (ONFH), the hip joint osteoarthritis (OA), and heterotopic ossification (HO); were compared between the two groups. Conversion to THR was also recorded as an outcome measure, analyzed by Kaplan–Meier curve and log-rank test.

Results
The mean operative time was similar in the two groups. The estimated blood loss of group B (435.7 ± 307.8 ml) was nearly double that of group A (233.1 ± 116.8 ml), although not statistically significant (P = 0.135). Radiographic outcomes including the quality of fracture reduction, ONFH, hip joint OA, and HO did not differ significantly between the two groups. There were also no significant differences between the two groups in terms of mean Merle d’Aubigné-Postel score at 1 year after injury and the log rank test of conversion to THR. One patient in group A had recurrent dislocation and underwent revision surgery, while one patient in group B requested removal of the trochanteric screws due to discomfort.

Conclusions
For the management of Pipkin type IV FH fractures, the modified Hardinge approach results in reduced blood loss, similar clinical and radiological outcomes compared with trochanteric osteotomy. The modified Hardinge approach appears to be an acceptable alternative to trochanteric flip osteotomy.
Costs analysis of Staged Versus Simultaneous Bilateral Total Knee and Hip Arthroplasty：a university affiliated hospital survey of 1579 Chinese patients
Authors: Hou Ji-Fei , Hu Chuan , Liu Yan-Zheng , Zhang Chi , Li Jing , Wang Yue , Li Cheng-Cheng , Zhang Yun 
Published: August 2019

Abstract
Background
Total knee arthroplasty (TKA) and total hip arthroplasty (THA) are required for many patients. No recent studies that concentrate on the costs for each of the payment items both for TKA and THA when comparing simultaneous bilateral procedures with staged arthroplasty. This study aimed to evaluate the medical costs, length of stay(LOS), blood transfusion, and in-hospital complications in patients undergoing simultaneous TKA or THA and staged TKA or THA.

Methods
A retrospective cohort study was conducted by analyzing 1,579 patients from a single institution. All patients who underwent primary bilateral TKA or THA from 2013 to 2018 were divided into simultaneous bilateral and staged bilateral groups. These two sub-groups were compared between three groups(TKA, THA, and pooled groups).

Results
All categories of medical costs, except for materials fees which were higher in the simultaneous bilateral THA and pooled groups but with no statistical significance, were lower in the simultaneous TKA, THA and pooled simultaneous groups. The total average medical costs in simultaneous and staged bilateral TKA groups were $15,535.621($12,362.542,$16,450.131)and $16,747.192($14,670.273, $18,036.289), (p <0.001), respectively; THA groups were $15,046.210 ($12,838.230, $17,026.529) and $16,126.808 ($14,575.031, $18,867.963), (p=0.003), respectively; pooled groups were $16,506.971 ($14,618.267, $18,185.414) and $15,467.561 ($12,432.783, $16,494.155), (p <0.001), respectively. The highest and lowest costs were materials fees and nursing care fees. No significant differences were found for complications between the two sub-groups in three groups. The simultaneous groups had a shorter LOS, a higher incidence of blood transfusion than staged groups whether it is TKA groups or THA or pooled groups.

Conclusions
These results indicate that simultaneous bilateral TKA and THA with a shorter LOS is more cost-effective than staged bilateral TKA and THA. Counts of complications were not affected by the choice for staged or simultaneous bilateral total arthroplasty. But blood transfusion was more prevalent in the simultaneous groups both for TKA and THA.
Two-year outcome of an eye that underwent hyperopic LASIK following inadvertent myopic SMILE lenticule in situ implantation
Authors: Zhao Jing , Shang Jianmin , Niu Lingling , Xu Haipeng , Yang Dong , Zhao Yu , Fu Dan , Zhou Xingtao 
Published: August 2019

Abstract
Background: This report describes a case in which hyperopic femtosecond laser-assisted in situ keratomileusis (FS-LASIK) was performed following small incision lenticule extraction (SMILE) lenticule in situ implantation.
Case presentation: The hyperopic left eye of a 46-year-old patient with refraction of +7.75 diopters sphere (DS)/-1.25 diopters cylinder (DC)  5° and corrected distance visual acuity (CDVA) of 20/50 mistakenly underwent the SMILE procedure for myopic astigmatism (-8.50 DS/-1.50 DC  175°) due to medical negligence. The extracted lenticule was subsequently re-implanted in situ. After 8 months, the left eye underwent FS-LASIK to correct hyperopia and astigmatism (+5.0 DS/-0.75 DC × 100°). Two years after FS-LASIK, corneal tomography showed no ectasia and microscopy revealed transparent cornea. The left eye exhibited CDVA of 20/50 with refraction of -0.75 DS/-0.25 DC × 165°.
Conclusions: SMILE lenticule in situ implantation offers a solution for corneal volume and thickness restoration. FS-LASIK provides feasible correction of refractive error following lenticule re-implantation. Future studies are needed for determining the effectiveness of the treatment.