bluuebunny/crossref_metadata_2025_split huggingface dataset:
Folding latency of fluorescent proteins affects the mitochondrial localization of fusion proteins
Authors: Kashiwagi Sayaka , Fujioka Yoichiro , Satoh Aya O. , Yoshida Aiko , Fujioka Mari , Nepal Prabha , Tsuzuki Atsushi , Aoki Ozora , Paudel Sarad , Sasajima Hitoshi , Ohba Yusuke 
Published: September 2019

ABSTRACTThe discovery of fluorescent proteins (FPs) has revolutionized cell biology. The fusion of targeting sequences to FPs enables the investigation of cellular organelles and their dynamics; however, occasionally, such fluorescent fusion proteins (FFPs) exhibit behavior different from that of the native proteins. Here, we constructed a color pallet comprising different organelle markers and found that FFPs targeted to the mitochondria were mislocalized when fused to certain types of FPs. Such FPs included several variants of Aequorea victoria green FP (aqGFP) and a monomeric variant of the red FP. Because the FFPs that are mislocalized include FPs with faster maturing or folding mutations, the increase in the maturation rate is likely to prevent their expected localization. Indeed, when we reintroduced amino acid substitutions so that the FP sequences were equivalent to that of wild-type aqGFP, FFP localization to the mitochondria was significantly enhanced. Moreover, similar amino acid substitutions improved the localization of mitochondria-targeted pHluorin, which is a pH-sensitive variant of GFP, and its capability to monitor pH changes in the mitochondrial matrix. Our findings demonstrate the importance of selecting FPs that maximize FFP function.
The ATG5 Interactome Links Clathrin Vesicular Trafficking With The ATG8 Lipidation Machinery For Autophagosome Assembly
Authors: Baines Kiren , Lane Jon D. 
Published: September 2019

ABSTRACTAutophagosome formation involves the sequential actions of conserved ATG family proteins that regulate the lipidation of the ubiquitin-like modifier ATG8 at the nascent isolation membrane. Although the molecular steps driving this process are well understood, the source of membranes supplied for the expanding autophagosome and their mode of delivery remain uncertain. Here, we have used quantitative SILAC-based proteomics to identify proteins that associate with the ATG12∼ATG5 conjugate that is crucial for ATG8 lipidation. Our datasets reveal a strong enrichment of regulators of clathrin-mediated vesicular trafficking, including clathrin heavy and light chains, and several clathrin adaptors. Also identified were PIK3C2A (a phosphoinositide 3-kinase involved in clathrin-mediated endocytosis) and HIP1R (a component of clathrin vesicles), and the absence of either of these proteins caused defects in autophagic flux in cell-based starvation assays. To determine whether the ATG12∼ATG5 conjugate reciprocally influences trafficking within the endocytic compartment, we captured the cell surface proteomes of autophagy-competent and autophagy-incompetent mouse embryonic fibroblasts under fed and starved conditions. Proteins whose surface expression increased contingent on autophagic capability included EPHB2, SLC12A4, and JAG1. Those whose surface expression was decreased included CASK, SLC27A4 and LAMP1. These data provide evidence for direct regulatory coupling between the ATG12∼ATG5 conjugate and the clathrin membrane trafficking system, and suggest candidate membrane proteins whose trafficking within the cell may be modulated by the autophagy machinery.
Service ecosystem well-being: conceptualization and implications for theory and practice
Authors: Frow Pennie , McColl-Kennedy Janet R. , Payne Adrian , Govind Rahul 
Published: September 2019

PurposeThis paper aims to conceptualize and characterize service ecosystems, addressing calls for research on this important and under-researched topic.Design/methodology/approachThe authors draw on four meta-theoretical foundations of S-D logic – resource integration, resource density, practices and institutions – providing a new integrated conceptual framework of ecosystem well-being. They then apply this conceptualization in the context of a complex healthcare setting, exploring the characteristics of ecosystem well-being at the meso level.FindingsThis study provides an integrated conceptual framework to explicate the nature and structure of well-being in a complex service ecosystem; identifies six key characteristics of ecosystem well-being; illustrates service ecosystem well-being in a specific healthcare context, zooming in on the meso level of the ecosystem and noting the importance of embedding a shared worldview; provides practical guidance for managers and policy makers about how to manage complex service ecosystems in their quest for improving service outcomes; and offers an insightful research agenda.Research limitations/implicationsThis research focuses on service ecosystems with an illustration in one healthcare context, suggesting additional studies that explore other industry contexts.Practical implicationsPractically, the study indicates the imperative for managing across mutually adapting levels of the ecosystem, identifying specific new practices that can improve service outcomes.Social implicationsExamining well-being in the context of a complex service ecosystem is critical for policymakers charged with difficult decisions about balancing the demands of different levels and actors in a systemic world.Originality/valueThe study is the first to conceptualize and characterize well-being in a service ecosystem, providing unique insights and identifying six specific characteristics of well-being.
White sanctuaries: race and place in art museums
Authors: Embrick David G. , Weffer Simón , Dómínguez Silvia 
Published: September 2019

Purpose
This paper examines the Art Institute of Chicago – a nationally recognized museum – as a white sanctuary, i.e., a white institutional space within a racialized social system that serves to reassure whites of their dominant position in society. The purpose of this paper is to highlight how museums create and maintain white spaces within the greater context of being an institution for the general public.

Design/methodology/approach
The empirical analysis of this study is based on collaborative ethnographic data collected over a three-year period of time conducted by the first two authors, and consists of hundreds of photos and hundreds of hours of participant observations and field notes. The data are analyzed using descriptive methods and content analyses.

Findings
The findings highlight three specific racial mechanisms that speak to how white spaces are created, recreated and maintained within nationally and internationally elite museums: spatiality, the policing of space, and the management of access.

Research limitations/implications
Sociological research on how white spaces are maintained in racialized organizations is limited. This paper extends to museums’ institutional role in maintaining white supremacy, as white sanctuaries.

Originality/value
This paper adds to the existing literature on race, place and space by highlighting three specific racial mechanisms in museum institutions that help to maintain white supremacy, white normality(ies), and serve to facilitate a reassurance to whites’ anxieties, fears and fragilities about their group position in society – that which helps to preserve their psychological wages of whiteness in safe white spaces.
Factors explaining a cost-based pricing essence
Authors: Amaral Juliana Ventura , Guerreiro Reinaldo 
Published: September 2019

PurposeEmpirical studies have found that cost-based pricing remains dominant in pricing practice and suggest that practice conflicts with marketing theory, which recommends value-based prices. However, empirical studies have yet to examine whether cost-plus formulas represent the pricing approach or essence.Design/methodology/approachThis study aims to address the factors that explain price setting whereby the cost-plus formula is not just the pricing approach but also the pricing essence. This examination is grounded in a survey conducted on 380 Brazilian industrial companies.FindingsThe results show that, for price-makers, the cost-based pricing essence is positively associated with four factors (two obstacles to deploying value-based pricing, company size and differentiation), but it is negatively related to one factor (premium pricing strategy). For price-takers, the cost-based pricing essence is positively associated with four factors (two obstacles to deploying value-based pricing, coercive isomorphism and use of full costs), but it is negatively related to five factors (one obstacle to deploying value-based pricing, company size, competitors’ ability to copy, normative isomorphism and experience).Originality/valueThe key contribution of this paper is demonstrating that cost-plus formulas do not go against the incorporation of competitors and value information. This study reveals that it is possible to set prices based on either value or competitors’ prices while simultaneously preserving the simplicity of the cost-plus formulas. Via the margin, firms may connect costs to information about competition and value. The authors also demonstrate the drawbacks of not segregating companies into price-makers and price-takers and an excessive focus on the pricing approach at the expense of pricing essence.
Organizational prerequisites for knowledge creation and sharing: empirical evidence from Serbia
Authors: Stojanović-Aleksić Vesna , Erić Nielsen Jelena , Bošković Aleksandra 
Published: September 2019

Purpose
Being mindful of the importance of organizational structure and organizational culture for knowledge management in companies, the purpose of this study is to investigate the organizational prerequisites for creating and sharing knowledge. The goals are to determine whether and to what extent the attributes of organic structure contribute to the creation and sharing of knowledge and to show that an organizational culture which supports knowledge stimulates the processes of knowledge creation and sharing.

Design/methodology/approach
The data for the empirical study was obtained through a survey of 150 respondents, employed in 30 companies from several industries, in the Republic of Serbia. The questionnaire was adapted to the needs of the study and was developed based on the theoretical knowledge and findings of several previous studies on processes of knowledge creation and knowledge sharing. A regression method was used to test all hypotheses.

Findings
The results show that both the organic structure and the organizational culture that support knowledge have positive effects on knowledge creation, while knowledge sharing is positively influenced only by the knowledge supporting culture of an organization.

Originality/value
This study contributes to organization studies and knowledge management theory because of the holistic approach taken with regards to the issue involved and the fact that it takes into account a large number of the significant characteristics of organizational structure and culture that are relevant to knowledge management processes. The findings could prove useful to managers when structuring an organization and shaping its culture to enhance knowledge management.
Paradoxes and partnerships: a study of knowledge exploration and exploitation in international development programmes
Authors: Lannon John , Walsh John N. 
Published: September 2019

PurposeThis paper aims to look at how organisational partnerships balance knowledge exploration and exploitation in contexts that are rife with paradoxes. It draws on paradox theory to examine the partnership’s response to the explore-exploit relationship.Design/methodology/approachA multiple interpretive case study was used to examine international partnerships in three African countries. These partnerships were between international (Northern-based) non-governmental organisations and local African non-governmental organisations.FindingsThe research finds that within the partnership, knowledge exploration and exploitation exist as a duality rather than a dualism. This is supported by the acceptance and confrontation of paradoxes of performing and belonging. However, macro-level paradoxes of organising linked to power, culture and epistemologies inhibit further effective confrontation of the explore-exploit paradox.Practical implicationsThe findings can help managers working in international development organisations to understand how learning is enabled and constrained in partnership-based programmes.Originality/valueThe study provides a novel contribution to knowledge management by applying the paradox perspective to the explore-exploit relationship. This paper extends previous work by drawing on the levels and repertoires present in the paradox perspective to understand how knowledge exploration and exploitation can be mutually reinforcing and can exist as a duality.
Improving travellers' trust in restaurant review sites
Authors: Anaya-Sánchez Rafael , Molinillo Sebastian , Aguilar-Illescas Rocío , Liébana-Cabanillas Francisco 
Published: September 2019

Purpose
This paper aims to focus on understanding how restaurant review sites generate trust among travellers and the effect of trust on the intention to visit and recommend restaurants while travelling.

Design/methodology/approach
The research model was assessed with data from a sample of 439 Spanish tourists using partial least squares structural equation modelling (PLS-SEM).

Findings
Information quality and source credibility are key for generating website trust. Website quality does not influence trust. Website trust is a key variable for explaining both intention to visit and recommend a restaurant, offline and online. Age is a significant moderator.

Research limitations/implications
This study expands the literature by assessing the antecedents and consequences of travellers’ trust in restaurant review sites during their trips.

Practical implications
Strategies are suggested for review sites, restaurants and destination managers.

Originality/value
This research enhances the knowledge of how consumers perceive restaurant review sites, and their behavioural intention while travelling.
The tourist experience life cycle: a perspective article
Authors: Dodds Rachel 
Published: September 2019

Purpose
The purpose of this paper is to review the past literature of the tourist experience and propose a new model of behavior.

Design/methodology/approach
It is proposed that understanding of the visitors experience from a development standpoint may be useful in understanding how tourism destinations or indeed products may be experienced so differently. Therefore, a tourist experience life cycle has been put forth as a model.

Findings
This model may help to outline how tourists, like destinations, also go through a life cycle. The implications may help destinations better understand the different motivations of their visitors.

Originality/value
While the literature has discussed the tourism experience and how the customer experience has changed, there has been little focus on the longitudinal development of how the actual tourist experiences a destination.
Airport management: a perspective article
Authors: Graham Anne 
Published: September 2019

Purpose
The purpose of this paper is to present an overall viewpoint on the past (1946-2020) and future (2020-2095) development of airport management.

Design/methodology/approach
The paper is based on a review of secondary literature.

Findings
The paper finds that the airport industry has transformed from a public utility to a modern enterprise, with private sector ownership, more competition, the expansion of commercial facilities and more diverse business models.

Originality/value
The paper provides a unique long-term and comprehensive view of past, current and future airport management, bringing together a number of linked developments, which is not found elsewhere.
Holistic theory development in tourism and hospitality: a perspective article
Authors: Tasci Asli D.A. 
Published: September 2019

Purpose
This opinion piece calls for a consolidation of theories through integrative literature reviews to set forth the path for holistic theory development in future research.

Design/methodology/approach
This is a perspective article.

Findings
It is difficult to substantiate the value of tourism and hospitality researchers’ contribution due to the fragmented nature of research because researchers typically conduct their studies in silos, sometimes completely detached from others who study similar or even the same concepts using different terminology.

Originality/value
This perspective article suggests critical and integrative literature reviews to consolidate concepts, models, theories, taxonomies and typologies for more holistic theories.
Emotional intelligence and tourist experience: a perspective article
Authors: Prentice Catherine 
Published: September 2019

PurposeThis paper aims to discuss how emotional intelligence can be incorporated into the tourism sector to enhance tourist experience.Design/methodology/approachThe paper reviews and synthetises current research on emotional intelligence and tourism research.FindingsEmotional intelligence is proposed to be incepted into the tourism context through its influence on the service encounter between tourism service representatives and tourists.Originality/valueThe paper provides a fresh view on tourist experience and suggests applying emotional intelligence into the tourism context to enhance tourist experience.
Co-creating tourism experiences through a traveler’s journey: a perspective article
Authors: Zhang Tingting 
Published: September 2019

Purpose
This perspective article aims to summarize research on value co-creation and tourism experience management from the past decades and to propose several future research priorities related to the topic.

Design/methodology/approach
This perspective article reviews the previous work and offers a process model on co-creating tourism experiences through a traveler’s journey. In addition, the article proposes five future research priorities related to value co-creation strategies in tourism experience management.

Findings
This perspective article depicts value co-creation activities during the pre-, mid-, and post-travel stages and proposes five research priorities: (1) big data and real-time co-creation will become ubiquitous in future travel experiences, (2) social media will continue to empower travelers throughout their travels, (3) human–robot interactions will increase for travelers’ co-creation activities, and (4) the diversity of travelers and younger generations (Generation Z) will impact co-created travel journeys.

Originality/value
Co-creation of tourism experiences has been increasing in the travel industry. It is necessary to review what has been said in the past and to project future research priorities and directions for travel academia and practitioners.
Tourism and on-site humour: a perspective article
Authors: Pabel Anja , Pearce Philip L. 
Published: September 2019

PurposeThis paper aims to outline major theoretical concepts relating to the tourism-humour relationship and provide commentary on opportunities for further research in this area.Design/methodology/approachPapers for this perspective research were sourced by conducting a systematic review which critically appraised relevant research on this topic to provide evidence on humour and its current use in tourism settings.FindingsHumour has received attention from many academic tribes and disciplines; however, investigations in the context of tourism are only in its early stages.Research limitations/implicationsThe paper is based on a select number of peer-reviewed literature studies on the topic of tourism and humour.Practical implicationsThe paper outlines some of the challenges that tourism operators face regarding authenticity, i.e. when the delivery of scripted humour is performed in such a way that it is considered spontaneous and meaningful.Originality/valueThe paper provides a brief overview of how humour is currently used in tourism settings and recommends future research opportunities to guide further studies into this topic.
Destination management: a perspective article
Authors: Fyall Alan , Garrod Brian 
Published: September 2019

Purpose
Destinations are focal points for tourist activity and thus for the study of tourism. They are, however, notoriously difficult to manage due to their complex systems of stakeholders. Such complexity implies that destinations are driven by a wide range of forces in their internal and external environments. The purpose of this paper is to highlight the diversity and depth of the challenges at play in destination management and, in doing so, review the primary contributions in the field.

Design/methodology/approach
This is a "perspective" paper (i.e. critical literature review).

Findings
The study of destinations remains an area of active research interest, with the many challenges that arise from within and external to destinations offering much food for thought with respect to their longevity as viable, sustainable and competitive places for tourists to visit. For the future, the need to view destinations as part of a wider system is paramount with them being inescapable from debates on urban planning, economic inequality, transportation and housing and the omnipresence of all things “smart”. Such debates need to incorporate both tourists and resident communities, as the well-being and quality of life of both groups is under threat in many destinations, particularly heritage and culturally rich city destinations around the world, where the term “overtourism” is increasingly heard.

Research limitations/implications
This study has implications for the integrated and more holistic management of tourist destinations.

Practical implications
This is a "perspective" paper, so it does not offer individual practical implications for destinations. Moreover, it offers a concise and precise summary of core studies in the field and provides a platform for a more future-looking critical debate on the sustainable management of tourist destinations.

Social implications
Looking ahead, destinations need to be considered as part of a wider system, one that is inclusive of urban planning, economic inequality, transportation and housing, and “smart” initiatives among others. Most importantly, the views of tourist and resident communities need to be considered and incorporated into future planning at the destination level with wellbeing and quality of life indicators being used to identify the real benefits of tourism to both communities.

Originality/value
Integrated and holistic forms of destination management is the way forward, with the exponential growth of technology, as well as the need to manage the exchange of knowledge and data at the destination level, critical to the sustainability of the competitive destination long into the future.