bluuebunny/crossref_metadata_2025_split huggingface dataset:
Free gastroepiploic lymph nodes and omentum flap for treatment of lower limb ulcers in severe lymphedema: Killing two birds with one stone
Authors: Di Taranto Giuseppe , Chen Shih‐Heng , Elia Rossella , Bolletta Alberto , Amorosi Vittoria , Sitpahul Ngamcherd , Chan Jeffrey CY , Ribuffo Diego , Chen Hung‐Chi 
Published: June 2019

AbstractBackground and ObjectivesIn patients with lymphedema, the disruption of the lymphatic network increases skin turgor and fibrosis of subcutaneous tissue, delays wound healing, causing recurrent ulcerations and infections. In these cases, management of ulcers can be challenging.MethodsBetween January 2016 and June 2018, patients presenting with lymphedema were enrolled at our Institution. We selected patients with severe lymphedema and ulcers of lower limbs and we performed a surgical approach, involving free gastroepiploic lymph nodes and omentum flap, harvested through laparoscopy.ResultsWe enrolled 135 patients presenting for lymphedema. Among them, 10 eligible cases underwent excision of the ulcer and reconstruction with omentum flap. Mean age was 57.8 years and average follow‐up 24.1 months. Circumferences and skin tonicity significantly decreased from the preoperative period. Lymphoscintigraphy showed improvement of the lymphatic drainage and restoration of lymphatic network. No episodes of infection were recorded in the postoperative period.ConclusionsOur combined procedure merges free flap techniques and lymphedema surgery: omentum covers the defect while providing a new source of lymph nodes, improving the lymphatic networks of the affected limb. This technique can highly increase the quality of life of the patient in a single‐stage operation with fast recovery and low donor site morbidity.
Application of Contrast‐Enhanced Ultrasound in the Diagnosis of Solid Pseudopapillary Tumors of the Pancreas: Imaging Findings Compared With Contrast‐Enhanced Computed Tomography
Authors: Xu Ming , Li Xiao‐ju , Zhang Xiao‐er , Pan Fu‐shun , Tan Yang , Huang Tong‐yi , Hong Yu , Huang Guang‐liang , Xie Xiao‐yan 
Published: June 2019

ObjectivesThe purpose of this study was to assess the characterization and usefulness of contrast‐enhanced ultrasound (CEUS) for diagnosing solid pseudopapillary tumors of the pancreas (SPTP) and compare the enhancement patterns with contrast‐enhanced computed tomography (CECT).MethodsForty‐three SPTP lesions proved by pathologic findings in 42 patients examined with CEUS and CECT were included in this study. The enhancement characteristics and typical CEUS features of the tumors were investigated. These characteristics were compared according to lesion sizes. The enhancement patterns of CEUS were compared with CECT.ResultsThe most common enhancement levels of SPTP in the early phase and late phase for CEUS were isoenhancement (19 of 43 [44.2%]) and hypoenhancement (32 of 43 [74.4%]), respectively. The 4 most common enhancement patterns were hypo‐hypo (16 of 43 [37.2%]), iso‐iso (11 of 43 [25.6%]), hyper‐hypo (8 of 43 [18.6%]), and iso‐hypo (8 of 43 [18.6%]) enhancement. For the 43 SPTP lesions, typical CEUS features such as lesion membrane, intralesional vessel, and intralesional compartmentalization enhancements were detected in 30 (69.8%), 27 (62.8%), and 10 (23.2%) cases. Compared with CECT, isoenhancement during the early phase and hypoenhancement during the late phase were the most common imaging characteristics of CEUS.ConclusionsLesion membrane, intralesional vessel, and intralesional compartmentalization enhancements are typical CEUS features of SPTP, especially for large lesions (lesion size ≥3.0 cm). Isoenhancement during the early phase and hypoenhancement during the late phase are the most common imaging characteristics of CEUS and CECT, making CEUS a viable alternative diagnostic method that is noninvasive.
Diversity, enumeration, and isolation of Arcobacter spp. in the giant abalone, Haliotis gigantea
Authors: Mizutani Yukino , Iehata Shunpei , Mori Tetsushi , Oh Ryota , Fukuzaki Satoshi , Tanaka Reiji 
Published: June 2019

AbstractArcobacter have been frequently detected in and isolated from bivalves, but there is very little information on the genus Arcobacter in the abalone, an important fishery resource. This study aimed to investigate the genetic diversity and abundance of bacteria from the genus Arcobacter in the Japanese giant abalone, Haliotis gigantea, using molecular methods such as Arcobacter‐specific clone libraries and fluorescence in situ hybridization (FISH). Furthermore, we attempted to isolate the Arcobacter species detected. Twelve genotypes of clones were obtained from Arcobacter‐specific clone libraries. These sequences are not classified with any other known Arcobacter species including pathogenic Arcobacter spp., A. butzleri, A. skirrowii, and A. cryaerophilus, commonly isolated or detected from bivalves. From the FISH analysis, we observed that ARC94F‐positive cells, presumed to be Arcobacter, accounted for 6.96 ± 0.72% of all EUB338‐positive cells. In the culture method, three genotypes of Arcobacter were isolated from abalones. One genotype had a similarity of 99.2%–100.0% to the 16S rRNA gene of Arcobacter marinus, while the others showed only 93.3%–94.3% similarity to other Arcobacter species. These data indicate that abalones carry Arcobacter as a common bacterial genus which includes uncultured species.
AEBP1, a prognostic indicator, promotes colon adenocarcinoma cell growth and metastasis through the NF‐κB pathway
Authors: Xing Yanwei , Zhang Zhiqiang , Chi Fengxu , Zhou Yang , Ren Shuo , Zhao Zhiwei , Zhu Yuekun , Piao Daxun 
Published: June 2019

AbstractThe abnormal expression of adipocyte enhancer binding protein 1 (AEBP1) has been implicated in the carcinogenesis and progression of various types of human tumors. However, the role of AEBP1 in colon adenocarcinoma (COAD) remains largely unelucidated. In this study, we explored the clinical significance and biological function of AEBP1 in COAD. We observed that AEBP1 was overexpressed in COAD tissues and cells and that the expression of AEBP1 was correlated with tumor size, the level of histologic differentiation, lymph node metastasis, and cancer stage in COAD patients. In addition, univariate and multivariate Cox regression analyses revealed that high AEBP1 expression suggested poor prognosis in COAD. Moreover, AEBP1 silencing suppressed COAD cell proliferation, migration, and invasion, whereas the upregulation of AEBP1 promoted these behaviors. Additionally, mechanistic studies further demonstrated that AEBP1 promoted COAD cell proliferation, migration, and invasion by upregulating the expression of matrix metalloproteinase‐2, vimentin, and TWIST whereas downregulating that of E‐cadherin through the nuclear factor‐κB pathway. Collectively, these data indicated that AEBP1 may be a new prognostic factor and a potential gene therapy target in COAD.
Ventral posterior substantia nigra iron increases over 3 years in Parkinson's disease
Authors: Bergsland Niels , Zivadinov Robert , Schweser Ferdinand , Hagemeier Jesper , Lichter David , Guttuso Thomas 
Published: June 2019

AbstractBackgroundParkinson's disease (PD) is characterized in part by the progressive accumulation of iron within the substantia nigra (SN); however, its spatial and temporal dynamics remain relatively poorly understood.ObjectivesThe objective of this study was to investigate spatial patterns and temporal evolution of SN iron accumulation in PD.MethodsA total of 18 PD patients (mean disease duration = 6.2 years) receiving dopaminergic therapy and 16 healthy controls were scanned with 3T MRI at baseline and 3 years later using quantitative susceptibility mapping, an indirect marker of iron content. Iron was assessed separately in the posterior SN and anterior SN at the ventral and dorsal levels of the SN. The results were corrected for the false discovery rate.ResultsA significant group effect was found for the ventral posterior SN (P < .001) and anterior SN (P = .042) quantitative susceptibility mapping as well as significant group x time interaction effects (P = .02 and P = .043, respectively). In addition, a significant intragroup change during 3 years of follow‐up was found only in the ventral posterior SN of PD (P = .012), but not healthy controls. No significant effects were detected for any dorsal SN measures. No associations were identified with clinical measures.ConclusionsWe found both cross‐sectional and longitudinal SN iron changes to be confined to its more ventral location in PD. Because pathology studies also show the ventral SN to degenerate early and to the greatest extent in PD, the assessment of iron levels by quantitative susceptibility mapping in this area may potentially represent a disease progression biomarker in PD. © 2019 International Parkinson and Movement Disorder Society
A reduced‐order extrapolated Crank–Nicolson collocation spectral method based on proper orthogonal decomposition for the two‐dimensional viscoelastic wave equations
Authors: Luo Zhendong , Jin Shiju 
Published: June 2019

AbstractIn order to reduce the order of the coefficient vectors of the solutions for the classical Crank–Nicolson collocation spectral (CNCS) method of two‐dimensional (2D) viscoelastic wave equations via proper orthogonal decomposition, we first establish a reduced‐order extrapolated CNCS (ROECNCS) method of the 2D viscoelastic wave equations so that the ROECNCS method has the same basis functions as the classical CNCS method and maintains all advantages of the classical CNCS method. Then, by means of matrix analysis, we discuss the existence, stability, and convergence for the ROECNCS solutions so that the theory analysis becomes very concise. Finally, we utilize some numerical experiments to validate that the consequences of numerical computations are accordant with the theoretical analysis such that the effectiveness and viability of the ROECNCS method are further verified. Therefore, both theory and method of this paper are new and totally different from the existing reduced‐order methods.
Frontal weld lines in injection‐molded short fiber‐reinforced PBT: Extensive microstructure characterization for mechanical performance evaluation
Authors: Baradi Mohamed B. , Cruz Camilo , Riedel Thomas , Régnier Gilles 
Published: June 2019

AbstractDifferent factors contribute to the weakness of weld lines (WLs) induced by injection molding such as unsuitable fiber orientation (FO), incomplete polymer matrix diffusion, voids and V‐notches. This study aims to characterize the contribution of each factor on the weakness of frontal WLs in a short glass fiber‐reinforced polybutylene‐terephthalate characterized by extensive X‐ray computed tomography and mechanical tensile testing assisted with digital image correlation. A reduction of 50% of the stress at break and almost 40% of the strain at break is observed despite the complete matrix healing at the WL interface and the absence of V‐notches. Frontal WLs induce a FO gradient starting 2 to 3 mm before the WL plane. The fibers in the WL region mainly orient in transverse‐to‐flow and thickness direction. This FO gradient localizes the deformations, which leads to failure at a strength near to the one of the unreinforced variant. Voids formation in frontal WLs seems to be driven by large gradients of FO and subsequent anisotropic shrinkage. In addition, this shrinkage behavior at the WL causes an increase of thickness. By applying higher packing pressures, the fibers orient more in flow direction at the core of the WL, leading to a higher tensile strength and a lower content of voids. Finally, we can conclude that the FO is the dominant factor controlling the mechanical performance in frontal WLs.
SGLT inhibitors for people with type 1 diabetes
Authors: Llano Andrea , McKay Gerry , Fisher Miles 
Published: June 2019

AbstractDespite significant advances in insulin therapy and monitoring technology, many with type 1 diabetes (T1DM) fail to achieve satisfactory glycaemic control and it remains a disease associated with significant morbidity and a reduced life expectancy. Adjunctive therapies which improve glycaemic control while reducing the risk of hypoglycaemia and weight gain are needed, and SGLT inhibitors have emerged as a potential candidate by virtue of their unique insulin‐independent mechanism of action. There is now substantial evidence for their efficacy in T1DM, but with an increased risk of diabetic ketoacidosis. Dapagliflozin and sotagliflozin have recently been approved for use in Europe for type 1 diabetes. SGLT inhibitors will not be suitable for all with T1DM so careful selection of individuals who may benefit is important, with strategies in place to mitigate the risk of ketoacidosis. Copyright © 2019 John Wiley & Sons.
Zostavax vaccine effectiveness among US elderly using real‐world evidence: Addressing unmeasured confounders by using multiple imputation after linking beneficiary surveys with Medicare claims
Authors: Izurieta Hector S. , Wu Xiyuan , Lu Yun , Chillarige Yoganand , Wernecke Michael , Lindaas Arnstein , Pratt Douglas , MaCurdy Thomas E. , Chu Steve , Kelman Jeffrey , Forshee Richard 
Published: June 2019

AbstractPurposeMedicare claims can provide real‐world evidence (RWE) to support the Food and Drug Administration's ability to conduct postapproval studies to validate products' safety and effectiveness. However, Medicare claims do not contain comprehensive information on some important sources of bias. Thus, we piloted an approach using the Medicare Current Beneficiary Survey (MCBS), a nationally representative survey of the Medicare population, to (a) assess cohort balance with respect to unmeasured confounders in a herpes zoster vaccine (HZV) effectiveness claims‐based study and (b) augment Medicare claims with MCBS data to include unmeasured covariates.MethodsWe reanalyzed data from our published HZV effectiveness Medicare analysis, using linkages to MCBS to obtain information on impaired mobility, education, and health‐seeking behavior. We assessed survey variable balance between the matched cohorts and selected imbalanced variables for model adjustment, applying multiple imputation by chained equations (MICE) to impute these potential unmeasured confounders.ResultsThe original HZV effectiveness study cohorts appeared well balanced with respect to variables we selected from the MCBS. Our imputed results showed slight shifts in HZV effectiveness point estimates with wider confidence intervals, but indicated no statistically significant differences from the original study estimates.ConclusionsOur innovative use of linked survey data to assess cohort balance and our imputation approach to augment Medicare claims with MCBS data to include unmeasured covariates provide potential solutions for addressing bias related to unmeasured confounding in large database studies, thus adding new tools for RWE studies.
Patient‐reported factors associated with the desire to continue taking sleep‐inducing drugs after hospital discharge: A survey of older adults
Authors: Heinemann Stephanie , Neukirchen Freya , Nau Roland , Hummers Eva , Himmel Wolfgang 
Published: June 2019

AbstractPurposeTo find out whether any prior experiences with sleep‐inducing drugs before hospitalization and positive experiences with these drugs during hospitalization influence a patient's wish to continue taking sleep‐inducing drugs after hospitalization.MethodsWe surveyed older hospital patients about use of sleep‐inducing drugs before, during, and after hospitalization and compared these answers with their hospital chart using the kappa statistic. The association between the wish to continue these drugs after discharge and the perceived benefits, experience of side effects, and prior experience with sleep‐inducing drugs was determined by multivariable logistic regression.ResultsAgreement between patient responses and the hospital file was high (κ = 0.7). Seventeen percent (83/483) of the participants reported prior experience before their hospital stay; 45% received a sleep‐inducing drug during hospitalization; 17% wished to continue taking them after discharge. Of the 400 patients who had no prior experience with sleep‐inducing drugs, 147 (37%) became first‐time users in the hospital, and 27% (40/147) of these wished to continue this medication after discharged. Strong predictors for this wish were the reduction of sleep onset problems (adjusted odds ratio, 6.26; 95% confidence interval, 2.38‐16.44) and any prior experience with sleep‐inducing drugs (4.08; 1.97‐8.48).ConclusionsMany older patients become first‐time users of sleep‐inducing drugs in the hospital. Especially the experience of sleep onset improvements influences the wish to continue sleep‐inducing drug use after discharge. Avoiding first‐time use should become a goal of hospital policy and be taken into account when weighing the benefits and risks of sleep‐inducing drugs.
Characterization of the GPR1/FUN34/YaaH protein family in the green microalga Chlamydomonas suggests their role as intracellular membrane acetate channels
Authors: Durante Lorenzo , Hübner Wolfgang , Lauersen Kyle J. , Remacle Claire 
Published: June 2019

AbstractThe unicellular green microalga Chlamydomonas reinhardtii is a powerful photosynthetic model organism which is capable of heterotrophic growth on acetate as a sole carbon source. This capacity has enabled its use for investigations of perturbations in photosynthetic machinery as mutants can be recovered heterotrophically. Fixation of acetate into cellular carbon metabolism occurs first by its conversion into acetyl‐CoA by a respective synthase and the generation of succinate by the glyoxylate cycle. These metabolic steps have been recently determined to largely occur in the peroxisomes of this alga; however, little is known about the trafficking and import of acetate or its subcellular compartmentalization. Recently, the genes of five proteins belonging to the GPR1/FUN34/YaaH (GFY) superfamily were observed to exhibit increased expression in C. reinhardtii upon acetate addition, however, no further characterization has been reported. Here, we provide several lines of evidence to implicate CrGFY1–5 as channels which share structural homology with bacterial succinate‐acetate channels and specifically localize to microbodies, which are surprisingly distinct from the glyoxylate cycle‐containing peroxisomes. We demonstrate structural models, gene expression profiling, and in vivo fluorescence localization of all five isoforms in the algal cell to further support this role.
Mild and Efficient Synthesis of Functionalized Carbazoles via a DBU‐Assisted Sequence Involving Cu‐ and Pd‐Catalyzed Coupling Reactions
Authors: Khan Aminur , Karim Rejaul , Dhimane Hamid , Alam Safiul 
Published: June 2019

AbstractPractical access to diversely functionalized carbazoles has been developed by consecutive Cu‐catalyzed Chan‐Lam N−arylation of various o‐iodoanilines and boronic acids, and Pd‐catalyzed intramolecular aryl C−H activation of 2‐iodo‐N‐arylanilines. Use of 1,8‐diazabicyclo[5.4.0]undec‐7‐ene (DBU) as base was found beneficial for both steps. In the Pd‐catalyzed C−H activation step, DBU acts as ligand as well as base, resulting in improved functional tolerance and higher yields than those observed with inorganic or other nitrogen bases. This DBU‐assisted sequence offers access to a variety of carbazoles with various electron‐donating and electron‐withdrawing substituents, including halogens or other reactive functional groups. Twenty‐seven cabazoles with various substitution paterns, including two naturally‐occurring carbazoles ‐ clausine L and clausine H ‐ have been successfully synthesized using these DBU‐promoted metal‐catalyzed coupling reactions.
Green Synthesized Manganese‐Cobaltite Nanospinel and Its Dye Removal Characteristics: Isothermal and Kinetic Studies
Authors: Tarighi Sara , Juibari Nafise Modanlou 
Published: June 2019

AbstractIn the present research, layered‐MnCo2O4 nanospinels have been prepared using Hibiscus rosa‐sinensis extract and characterized by Fourier transforms infrared spectroscopy (FTIR), X‐ray powder diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), dynamic light scattering (DLS), Brunauer‐Emmett‐Teller (BET), Barrett‐Joyner‐Halenda (BJH) techniques and zeta potential. The effect of the spinel nanoparticles on methyl orange and methylene blue removal from aquatic solutions were investigated through UV/Vis absorption. The influence of reaction parameters including time, mass of adsorbent, pH and the initial concentration of the dyes have been examined on sorption process. MnCo2O4 nanoparticles showed excellent activity on dyes elimination from water and the removal efficiency for methyl orange and methylene blue were 93 and 96%, respectively. The adsorption kinetics revealed a pseudo‐second‐order rate law, revealing the chemical sorption as the rate‐limiting step. The adsorption process were well‐matched with the Langmuir adsorption model. The maximum adsorption capacity was 100.01 and 104.16 mgg−1 for methyl orange and methylene blue, respectively.
Ethoxylated Head of Chalcones as a New Class of Multi‐Targeted MAO Inhibitors
Authors: Lakshminarayanan Balasubramanian , Baek Seung Cheol , Lee Jae Pil , Kannappan Nagappan , Mangiatordi Giuseppe Felice , Nicolotti Orazio , Subburaju Thillainayagam , Kim Hoon , Mathew Bijo 
Published: June 2019

AbstractA series of eleven ethoxysubstituted chalcones (E1‐E11) were synthesized and investigated for their inhibitory potential towards human recombinant monoamine oxidase A and B (hMAO−A and hMAO−B, respectively) and acetylcholinesterase (AChE). IC50 values of 4.63 ± 0.15 and 0.053 ± 0.003 μM were obtained for MAO−A and MAO−B, respectively, by the most interesting compound (2E)‐1‐(4‐ethoxyphenyl)‐3‐(4‐fluorophenyl)prop‐2‐en‐1‐one (E7), and it was characterized by a high selectivity index (SI=87.4) for MAO−B. Inhibitions by E7 against MAO−A and MAO−B were recovered (75.9 and 74.5%, respectively) to near the levels of reversible references (77.1 and 77.4%, respectively). The inhibition modes of E7 for MAO−A and MAO−B were competitive with Ki values of 2.65 ± 0.064 and 0.011 ± 0.0011 μM, respectively. Compounds (2E)‐1‐(4‐ethoxyphenyl)‐3‐(4‐ethylphenyl) prop‐2‐en‐1‐one (E10) and (2E)‐1‐(4‐ethoxyphenyl)‐3‐[4‐(trifluoromethyl)phenyl]prop‐2‐en‐1‐one (E11) showed good inhibitions against AChE with IC50 values of 2.86 ± 0.041 and 3.23 ± 0.0073 μM, respectively. A combined molecular docking/MM‐GBSA approach was used that employed quantum mechanics (QM) partial charges; this technique revealed the molecular rationale behind the observed MAO−B selectivity for this molecular series. Taken together, these results indicate that E7 is a potent, selective and reversible competitive inhibitor of MAO−B with moderately potent AChE inhibitory activity that has potential as a multi‐targeting drug