bluuebunny/crossref_metadata_2025_split huggingface dataset:
Delivery of Encapsulated Intelligent Engineered Probiotic for Inflammatory Bowel Disease Therapy
Authors: Zou Zhen‐Ping , Cai Zhihao , Zhang Xiao‐Peng , Zhang Donghui , Xu Chu‐Ying , Zhou Ying , Liu Runhui , Ye Bang‐Ce 
Published: December 2024

AbstractEngineered bacterial therapy holds enormous potential for treating intestinal diseases, employing synthetic biology techniques to achieve localized drug delivery within intestines. However, effective delivery of engineered bacteria to lesion sites and ensuring sustained colonization remain challenging. Here, a mucus encapsulated microsphere gel (MM) delivery system is developed to encapsulate genetically engineered bacteria capable of detecting and treating enteritis. The MM delivery system features an external mucosal coating composed of hyaluronic acid and epigallocatechin gallate, along with internal microspheres of highly biocompatible polyserine modified alginates encapsulating with the engineered probiotics. The MM delivery system effectively protects engineered bacteria harsh environment in stomach and significantly improves intestinal adhesion of the probiotics, extending colonization up to 24 h, and does not affect the entry of biomarker or release of Avcystatin. It exhibits notable diagnostic and therapeutic efficacy in inflammatory bowel disease models, thus facilitating the advancement of live biotherapeutic products toward clinical application.
Metal Doping Enabling Defective CoMo‐Layered Double Hydroxide Nanosheets as Highly Efficient Photosensitizers for NIR‐II Photodynamic Cancer Therapy
Authors: Yang Yu , Hu Tingting , Zhao Kexin , Wang Yi‐Chi , Zhu Yanfang , Wang Shibo , Zhou Zhan , Gu Lin , Tan Chaoliang , Liang Ruizheng 
Published: December 2024

AbstractPhotodynamic therapy (PDT) is attracting widespread attention as a promising strategy for tumor treatment. However, the efficacy of PDT is severely limited by the insufficient tissue penetration depth of the light source and low reactive oxygen species (ROS) generation efficiency. Herein, the metal doping strategy is reported to construct a series of defect‐rich M‐doped amorphous CoMo‐layered double hydroxide (a‐M‐CoMo‐LDH, M = Mn, Cu, Al, Ni, Mg, Zn) photosensitizers (PSs) for NIR‐II PDT. Especially, M‐doped CoMo‐LDH nanosheets are synthesized through a simple hydrothermal method and then etched by acid treatment to prepare defect‐rich a‐M‐CoMo‐LDH nanosheets. Under NIR‐II 1270 nm laser irradiation, the defect‐rich a‐Zn‐CoMo‐LDH nanosheets exhibit the optimal PDT performance compared with other a‐M‐CoMo‐LDH nanosheets, and also possess much higher ROS production activity (3.9 times) than that of the pristine a‐CoMo‐LDH, with a singlet oxygen quantum yield up to 1.86, which is the highest among all the reported PSs. After polyethylene glycol (PEG) modification, the a‐Zn‐CoMo‐LDH‐PEG nanosheets can function as an effective inorganic PS for PDT, effectively inducing cell apoptosis in vitro and eradicating tumors in vivo. Notably, transcriptome sequencing analysis and further molecular validation highlight the critical role of the apoptotic/p53/AMPK/oxidative phosphorylation signaling pathways in a‐Zn‐CoMo‐LDH‐PEG‐induced cancer cell apoptosis.
The First Kr‐Selective Carbon Molecular Sieve for Inverse Adsorption of Krypton Over Xenon at Ambient Temperature
Authors: Chen Fuqiang , Zheng Fang , Huang Xinlei , Chu Zhe , Sun Haoran , Yang Liu , Yang Qiwei , Zhang Zhiguo , Ren Qilong , Bao Zongbi 
Published: December 2024

AbstractThe efficient adsorption‐based separation of krypton (Kr) and xenon (Xe) is of paramount importance but is challenged by their similar physicochemical properties. While carbon adsorbents are theoretically promising for Kr/Xe sieving, practical success has remained elusive. Here, a series of ultramicroporous carbon molecular sieves synthesized from sucrose‐derived hydrochar is reported. The study employs careful characterization and controlled thermal pyrolysis to tailor ultramicropore formation and elucidate the evolution of the carbon framework. The leading material, C‐Suc‐750, has an ideal pore size of ≈4.0 Å. In particular, C‐Suc‐750 has achieved a remarkable Kr/Xe uptake ratio of 39.3 at ambient conditions, setting a new benchmark for selective Kr adsorption and molecular sieving of Kr/Xe. Breakthrough experiments further confirm the superior molecular sieving performance of C‐Suc‐750, highlighting its potential for Kr recovery in nuclear waste treatment. Moreover, molecular dynamics (MD) simulations demonstrate the critical role of narrow slit‐pore of the carbon molecular sieve in molecular sieving separation of Kr/Xe, providing insights into the mechanism driving this selectivity.
Tailoring Non‐Covalent Interaction Via Single Atom to Boost Interfacial Charge Transfer Toward Photoelectrochemical Water Oxidation
Authors: Zhang Chuanqi , Wang Yueyue , Sun Wenming , Hua Zhaorui , Zhang Zicong , Gong Shuyan , Wang Dingsheng , Tian Yang 
Published: December 2024

AbstractPhotoelectrochemical (PEC) water splitting for hydrogen generation holds immense potential for addressing environmental and energy crises. Tailoring non‐covalent interaction via a single atom is anticipated to realize prominent hole extracting facilitating PEC performance, but it has never been reported. In this study, single atom Co‐N4 is coordinated with 5‐fluoroanthranilic acid (FAA) molecules, then used as a non‐covalent hole‐extracting layer on a BiVO4 substrate. Experiments including X‐ray absorption fine spectra, Kelvin probe force microscopy, transient absorption, and theoretical calculation demonstrate the FAA coordination alters the local configuration of the central Co atom, adjusting the interfacial non‐covalent interaction, thereby reducing the barrier of charge transfer between BiVO4 and the hole‐extracting layer. Consequently, photogenerated carriers are more effectively separated, and the PEC water oxidation performance is significantly enhanced with the photocurrent density of 5.47 mA cm−2 at 1.23 V versus RHE, much higher than those of previously reported BiVO4 photoanodes composited with porphyrin‐based compounds. Experiments and theoretical simulation confirm that the boosted PEC performance originates from exceptional interfacial charge transfer rather than surface catalysis dynamic. This study provides an efficient strategy for tailoring non‐covalent interaction by regulating single‐atom coordination and promoting hole extract to boost PEC water oxidation activity.
GFRα1 Promotes Axon Regeneration after Peripheral Nerve Injury by Functioning as a Ligand
Authors: Suzuki Tomoaki , Kadoya Ken , Endo Takeshi , Yamasaki Miwako , Watanabe Masahiko , Iwasaki Norimasa 
Published: December 2024

AbstractThe neurotrophic factor, Glial cell line derived neurotrophi factor (GDNF), exerts a variety of biological effects through binding to its receptors, GDNF family receptor alpha‐1 (GFRα1), and RET. However, the existence of cells expressing GFRα1 but not RET raises the possibility that GFRα1 can function independently from RET. Here, it is shown that GFRα1 released from repair Schwann cells (RSCs) functions as a ligand in a GDNF‐RET‐independent manner to promote axon regeneration after peripheral nerve injury (PNI). Local administration of GFRα1 into injured nerve promoted axon regeneration, even more when combined with GDNF blockade. GFRα1 bound to a receptor complex consisting of NCAM and integrin α7β1 of dorsal root ganglion neurons in a GDNF‐RET independent manner. This is further confirmed by the Ret Y1062F knock‐in mice, which cannot transmit most of GDNF‐RET signaling. Finally, local administration of GFRα1 into injured sciatic nerve promoted functional recovery. These findings reveal a novel role of GFRα1 as a ligand, the molecular mechanism supporting axon regeneration by RSCs, and a novel therapy for peripheral nerve repair.
Co‐Translational Deposition of N6‐Acetyl‐L‐Lysine in Nascent Proteins Contributes to the Acetylome in Mammalian Cells
Authors: Guo Dingyuan , Li Nan , Zhang Xiaoyan , Zhou Runxin , He Jie , Ding Xiao‐Ping , Yu Weixing , Tong Fuqiang , Yin Sibi , Wang Yu , Xu Xin , Wang Long , Fan Mingzhu , Feng Shan , Liu Ke , Tang Ke , Ouyang Zhuqing , Guo Yusong R , Wang Yugang 
Published: December 2024

AbstractN6‐acetyl‐L‐lysine residue is abundant in dietary protein but little is known about its potential influences on the diet‐consumers. Herein, it is reported that Lysyl‐tRNA synthetase (KARS) mediates co‐translational deposition of diet‐derived N6‐acetyl‐L‐lysine (AcK) in nascent proteins to contribute to the acetylome in cells. Acetylated dietary protein is a direct source of AcK that can widely and substantially regulate the acetylome in multiple organs of mice. By analyzing the mechanisms underlying AcK contributing to the acetylome in mammalian cells, it is found that KARS can utilize AcK as an alternative substrate to produce N6‐acetyl‐l‐lysyl‐tRNA. The crystal structure of KARS in complex with AcK at 2.26 Å resolution shows that AcK shares the same substrate‐binding pocket as L‐lysine, allowed by a sidechain flip of Tyr499. The generated N6‐acetyl‐L‐lysyl‐tRNA introduces AcK into growing nascent polypeptide and results in protein acetylation, including the regions buried inside folded proteins that are post‐translational modification (PTM)‐inaccessible and functionally important. This undocumented protein modification mechanism is inherently different from PTM and termed as co‐translational modification (coTM). It is expected to extend the repertoire of acetylome and improve the understanding of protein modification mechanisms in cells.
Tailoring Borate Mediator Species Enables Industrial CO Production with Improved Overall Energy Efficiency by Sustainable Molten Salt CO2 Electrolysis
Authors: Li Xinyu , Deng Bowen , Du Kaifa , Li Wenmiao , Chen Di , Qu Xin , Pang Fangzhao , Zhang Xiaodan , Zha Hao , Yin Huayi , Wang Dihua 
Published: December 2024

AbstractThe electrochemical conversion of CO2 into CO represents a promising strategy for mitigating excessive global greenhouse gas emissions. Nevertheless, achieving industrial‐scale electrochemical CO2‐to‐CO conversion with enhanced selectivity and reduced energy consumption presents significant challenges. In this study, a borate‐enhanced molten salt process for CO2 capture and electrochemical transformation is employed, achieving over 98% selectivity for CO and over 55% energy efficiency without the necessity for complex and costly electrocatalysts. Cathodic CO2 electro‐reduction (CO2ER) with the anodic oxygen evolution reaction (OER) at an overall current density of 500 mA cm−2 using non‐nanostructured transition‐metal plate electrodes at 650 °C is coupled. By regulating the electrolyte's oxo‐basicity with earth‐abundant borax (Na2B4O7), a borate‐enhanced electrolyte is established that accelerates the overall electrochemical reaction efficiently. This system involved a series of well‐designed target borate species (BO33−, BO2−, and B4O72−) that acted as mediators shuttling between the cathode and anode, favoring CO as the primary cathodic product. Manipulating the atmosphere above the anode facilitated a spontaneous transformation of borates, further enhancing OER performance with long‐term operational stability over a cumulative period of 50 h, while also reducing overall energy consumption. This work presents a cost‐effective strategy for the industrial‐scale production of CO derived from CO2, contributing to a lower carbon footprint by establishing a sustainable borate‐mediated closed loop.
Automatic Quantitative Analysis of Internal Quantum Efficiency Measurements of GaAs Solar Cells Using Deep Learning
Authors: Abdullah‐Vetter Zubair , Wright Brendan , Wu Tien‐Chun , Shakiba Ali , Hameiri Ziv 
Published: December 2024

AbstractA solar cell's internal quantum efficiency (IQE) measurement reveals critical information about the device's performance. This information can be obtained using a qualitative analysis of the shape of the curve, identifying and attributing current losses such as at the front and rear interfaces, and extracting key electrical and optical performance parameters. However, conventional methods to extract the performance parameters from IQE measurements are often time‐consuming and require manual fitting approaches. While several methodologies exist to extract those parameters from silicon solar cells, there is a lack of accessible approaches for non‐silicon cell technologies, like gallium arsenide cells, typically limiting the analysis to only the qualitative level. Therefore, this study proposes using a deep learning method to automatically predict multiple key parameters from IQE measurements of gallium arsenide cells. The proposed method is demonstrated to achieve a very high level of prediction accuracy across the entire range of parameter values and exhibits a high resilience for noisy measurements. By enhancing the quantitative analysis of IQE measurements, the method will unlock the full potential of quantum efficiency measurements as a powerful characterization tool for diverse solar cell technologies.
Complete Genomic Landscape Reveals Hidden Evolutionary History and Selection Signature in Asian Water Buffaloes (Bubalus bubalis)
Authors: Si Jingfang , Dai Dongmei , Gorkhali Neena Amatya , Wang Mingshan , Wang Sheng , Sapkota Saroj , Kadel Ram Chandra , Sadaula Amir , Dhakal Aashish , Faruque Md Omar , Omar Abdullah Ibne , Sari Eka Meutia , Ashari Hidayat , Dagong Muhammad Ihsan Andi , Yindee Marnoch , Rushdi Hossam E. , Elregalaty Hussein , Amin Ahmed , Radwan Mohamed A. , Pham Lan Doan , Hulugalla W. M. M. P. , Silva G. L. L. Pradeepa , Zheng Wei , Mansoor Shahid , Ali Muhammad Basil , Vahidi Farhad , Al‐Bayatti Sahar Ahmed , Pauciullo Alfredo , Lenstra Johannes A. , Barker J. Stuart F. , Fang Lingzhao , Wu Dong‐Dong , Han Jianlin , Zhang Yi 
Published: December 2024

AbstractTo identify the genetic determinants of domestication and productivity of Asian water buffaloes (Bubalus bubalis), 470 genomes of domesticated river and swamp buffaloes along with their putative ancestors, the wild water buffaloes (Bubalus arnee) are sequenced and integrated. The swamp buffaloes inherit the morphology of the wild buffaloes. In contrast, most river buffaloes are unique in their morphology, but their genomes cluster with the wild buffaloes. The levels of genomic diversity in Italian river and Indonesian swamp buffaloes decrease at opposite extremes of their distribution range. Purifying selection prevented the accumulation of harmful loss‐of‐function variants in the Indonesian buffaloes. Genes that evolved rapidly (e.g., GKAP1) following differential selections in the river and swamp buffaloes are involved in their reproduction. Genes related to milk production (e.g., CSN2) and coat color (e.g., MC1R) underwent strong selections in the dairy river buffaloes via soft and hard selective sweeps, respectively. The selective sweeps and single‐cell RNA‐seq data revealed the luminal cells as the key cell type in response to artificial selection for milk production of the dairy buffaloes. These findings show how artificial selection has been driving the evolutionary divergence and genetic differentiation in morphology and productivity of Asian water buffaloes.
Molecular Programming Design of Glyconucleic Acid Aptamer with High Stability
Authors: Han Yongqi , Zhang Rongjun , Bao Hong‐Liang , Yang Mei , Gao Yuan , Gao Xiaobo , Wang Ruowen , Tan Weihong , Ji Ding‐Kun 
Published: December 2024

AbstractFunctional nucleic acids (FNAs), possessing specific biological functions beyond their informational roles, have gained widespread attention in disease therapeutics. However, their clinical application is severely limited by their low serum stability in complex physiological environments. In this work, a precise molecular programming strategy is explored to prepare glyconucleic acid aptamers (GNAAs) with high serum stability. Four glyconucleic acid modules compatible with commercial solid‐phase synthesis are designed and synthesized. Through precise molecular design, the accurate modification of four different carbohydrate ligands at specific sites of DNA aptamers is achieved. It is demonstrated that glycosylation modification can significantly increase DNA aptamers’ serum stability while maintaining their structures and high affinity. The stabilization effect is superior to that of currently commonly used commercial chemical modifications. Moreover, it is confirmed that this approach displays insignificant effects on the DNA aptamers’ tumor‐targeting ability and metabolism in vivo. This method offers a simple, economical, and efficient strategy for precise glycosylation modification of nucleic acids. This allows to prepare glycosyl functional nucleic acids with high serum stability, which can expand the application scope of functional nucleic acids and promote the practical transformation of functional nucleic acids.
Accurately Tunable AuNC‐ZIF Content Architecture Based on Coordination‐Dissociation Mechanism Enables Highly Brightness Dual‐Site Fluorescent Biosensor
Authors: Chen Junyang , Wang Yuqian , Shen Runpu , Li Wei , Gao Sainan , Xiao Zhikang , Lv Qiyan , Song Xiaojie , Xu Jianzhong , Xu Gaoxiang , Cui Huifang , Li Zhaohui 
Published: December 2024

AbstractThe quantum yield and fluorescence intensity of gold nanocluster (AuNC) nanocarriers are critical parameters for developing ultrasensitive biosensors. In this study, AuNCs‐zeolitic‐imidazolate‐framework (Au‐ZIF) nanocomposites are systematically constructed by impregnating AuNCs onto the ZIF‐8 surface through a coordination‐dissociation mechanism, resulting in a dual‐site fluorescence‐loaded structure. In this configuration, AuNCs are anchored to the external surface while the integrity of the inner cavity remains intact. The surface of ZIF‐8 induces a confinement effect on the configuration and electrons of AuNCs, significantly enhancing luminescence (18‐fold increase). The quantum yield of AuNCs exhibits an increase of more than 13‐fold, from 2.80% to 38.1%. This approach demonstrates broad applicability and maintains strong fluorescence across different ZIFs. Additionally, a novel nanocomposite, Au‐ZIF@carbon‐dots (CDs), is synthesized by encapsulating CDs into the inner cavity of Au‐ZIF. A ratiometric fluorescence detection platform is subsequently developed and incorporated into hydrogels for the quantitative detection of the pesticide triazophos. By employing an image‐processing algorithm, quantitative detection is achieved with a detection limit of 0.07 ng mL⁻1. The findings provide crucial insights into the relationship between the assembly and performance of AuNCs and ZIFs, offering guidance for designing ultrasensitive multifunctional biosensors applicable in the field of biosensing.
Photo‐Induced Synthesis of Ytterbium and Manganese‐Doped CsPbCl3 Nanocrystals for Visible to Near‐Infrared Photoluminescence with Negative Thermal Quenching
Authors: Fang Xiaochen , Chen Zhuo , Leung Mandy Hei Man , Zheng Biao , Wang Liwei , An Meng , Asakura Yusuke , Yamauchi Yusuke , Yuan Zhanhui 
Published: December 2024

AbstractRare‐earth‐doped all‐inorganic perovskite applications for near‐infrared (NIR) emission are crucial for the construction of the next generation of intelligent lighting sources. However, the preparation of rare‐earth‐doped all‐inorganic perovskite is complex, and difficult to control, and the issue of thermal quenching poses significant challenges to its practical application. Here, in order to address these issues, a convenient photo‐induced synthesis method for CsPbCl3:Mn/Yb nanocrystals (NCs) is proposed by decomposing carbon tetrachloride with 365 nm light to provide chloride ions and regulate the formation of perovskite at room temperature. The negative thermal quenching in the NIR emission is achieved through the energy transfer between Mn and Yb. The emission intensity of Yb enhances 3.2 times when the temperature rises to ≈427 K. Furthermore, with the help of the orange emission from the Mn2+ ions and the NIR emission from the Yb3+ ions, visible to NIR light emitting diode (LED) devices are constructed and applied in orange light illumination and night vision imaging. This study enriches the preparation methods and chemical research on perovskite doping, which may open up new opportunities for the widespread application of perovskite‐based materials or device engineering.
Grain Boundaries Control Lithiation of Solid Solution Substrates in Lithium Metal Batteries
Authors: Aota Leonardo Shoji , Jung Chanwon , Zhang Siyuan , Büyükuslu Ömer K. , Saksena Aparna , Hatipoglu Ezgi , Yadav Poonam , Singh Mahander Pratap , Chen Xinren , Woods Eric , Scheu Christina , Kim Se‐Ho , Raabe Dierk , Gault Baptiste 
Published: December 2024

AbstractThe development of sustainable transportation and communication systems requires an increase in both energy density and capacity retention of Li‐batteries. Using substrates forming a solid solution with body‐centered cubic Li enhances the cycle stability of anode‐less batteries. However, it remains unclear how the substrate microstructure affects the lithiation behavior. Here, a correlative, near‐atomic scale probing approach is deployed through combined ion‐ and electron‐microscopy to examine the distribution of Li in Li‐Ag diffusion couples as model system mimicking high current densities. It is revealed that Li regions with over 93.8% at.% nucleate within Ag at random high‐angle grain boundaries, whereas grain interiors are not lithiated. The role of kinetics and mechanical constraint from the microstructure over equilibrium thermodynamics in dictating the lithiation process is evidenced. The findings suggest that grain size and grain boundary character are critical to enhance the electrochemical performance of interlayers/electrodes, particularly for improving lithiation kinetics and hence reducing dendrite formation.
GLP‐1 Receptor Agonists Alleviate Diabetic Kidney Injury via β‐Klotho‐Mediated Ferroptosis Inhibition
Authors: Tian Shasha , Zhou Saijun , Wu Weixi , lin Yao , Wang Tongdan , Sun Haizhen , A‐Ni‐Wan A‐Shan‐Jiang , Li Yaru , Wang Chongyang , Li Xiaogang , Yu Pei , Zhao Yanjun 
Published: December 2024

AbstractSemaglutide (Smg), a GLP‐1 receptor agonist (GLP‐1RA), shows renal protective effects in patients with diabetic kidney disease (DKD). However, the exact underlying mechanism remains elusive. This study employs transcriptome sequencing and identifies β‐Klotho (KLB) as the critical target responsible for the role of Smg in kidney protection. Smg treatment alleviates diabetic kidney injury by inhibiting ferroptosis in patients, animal models, and HK‐2 cells. Notably, Smg treatment significantly increases the mRNA expression of KLB through the activation of the cyclic adenosine monophosphate (cAMP) signaling pathway, specifically through the phosphorylation of protein kinase A (PKA) and cAMP‐response element‐binding protein (CREB). Subsequently, the adenosine monophosphate‐activated protein kinase (AMPK) signaling pathway is activated, reprograming the key metabolic processes of ferroptosis such as iron metabolism, fatty acid synthesis, and the antioxidant response against lipid peroxidation. Suppression of ferroptosis by Smg further attenuates renal inflammation and fibrosis. This work highlights the potential of GLP‐1RAs and KLB targeting as promising therapeutic approaches for DKD management.