bluuebunny/crossref_metadata_2025_split huggingface dataset:
Proteomic Profiling of Mouse Helper T Cell Differentiation
Authors: van den Ham Henk‐Jan , Binai Nadine A. , Zaaraoui‐Boutahar Fatiha , Heck Albert J. R. , Andeweg Arno C. 
Published: February 2019

AbstractHelper T cell differentiation is a key process in the regulation of adaptive immune responses. Here, mouse Th1 and Th2 cells are profiled using high‐throughput proteomics to increase the understanding of the molecular biology of Th differentiation to support the design of prophylactic and therapeutic intervention strategies for (infectious) diseases. Protein profiling of Th1/Th2 differentiated cells results in the quantification of almost 6000 proteins of which 41 are differentially expressed at FDR < 0.1, and 19 at the FDR < 0.05 level, respectively. Differential protein expression analysis identifies a number of the expected canonical Th differentiation markers, and gene set analysis using the REACTOME database and a hypergeometric test (FDR < 0.05) confirms that helper T cell pathways are the top sets that are differentially expressed. Additionally, by network analysis, many differentially expressed proteins are associated with the Th1 and Th2 pathways. Data are available via PRIDE database with identifier PXD004532.
Integration of Metal Nanoparticles into Metal–Organic Frameworks for Composite Catalysts: Design and Synthetic Strategy
Authors: Li Bo , Ma Jian‐Gong , Cheng Peng 
Published: February 2019

AbstractMetal–organic frameworks (MOFs) are constructed by periodically alternate metal ions with organic ligands, which offer structural diversity and a wide range of interesting properties as an attractive classification of crystalline porous materials. Integration of MOFs with other size‐limited functional centers can supply new multifunctional composites, which exhibit both the properties of the components and new characteristics due to the combination of MOFs with the selected loadings. In recent years, integration of metal/metal oxide nanoparticles (MNPs) into MOFs to form the composite catalysts has attracted considerable attention due to the superior performance. In this review, the latest studies and up‐to‐date developments on the design and synthetic strategy of new MNP@MOF composite catalysts are specifically highlighted. Both the achievements and problems are evaluated and proposed, and the opportunities and challenges of MNP@MOF composite catalysts are discussed.
A review of the application of the name Quercus dalechampii
Authors: Di Pietro Romeo , Viscosi Vincenzo , Peruzzi Lorenzo , Fortini Paola 
Published: February 2019

AbstractEuropean taxonomists are divided regarding Quercus dalechampii, considering it either: (1) a distinct species close to Q. petraea, (2) a distinct species close to Q. pubescens, or (3) falling within the morphological variability of previously described species, including Q. petraea and Q. pubescens. These taxonomic disagreements are largely due to inconsistencies between the description of Q. dalechampii and much of its original material. In order to resolve these differing interpretations, all available original material was examined and a lectotype, superseding a previous neotype, was chosen from this material. The original material for the name consisted of mixed material belonging both to the Q. petraea complex and the Q. pubescens complex. The lectotype designated here is characterized by pubescent leaves and belongs to the Q. pubescens complex.
Nrf2 mediates the protective effect of edaravone after chlorpyrifos‐induced nervous system toxicity
Authors: Shou Limeng , Bei Yun , Song Ying , Wang Libo , Ai Liyao , Yan Qinying , He Wei 
Published: February 2019

AbstractWe aim to confirm the impairment of chlorpyrifos (CPF) in PC12 cells, evaluate the protective effect of edaravone on CPF‐induced injury, and try to unravel its underlying mechanism perspective from Nrf2 signaling pathway. Viability of PC12 cells treated with CPF and edaravone (Ed) were evaluated by MTT assay. Cell apoptosis was observed by the Hoechst 33342 stain. The level of reactive oxygen species (ROS), the content of malondialdehyde (MDA), and the activity of superoxide dismutase (SOD) were detected to evaluate the oxidative stress injury. The expression of Nrf2 was detected by Western blot; profoundly, RNA interference was conducted to construct Nrf2 gene knockdown PC12 cells and to uncover its underlying mechanism. MTT results showed CPF injured PC12 cells in a concentration‐dependent manner. Increased ROS and MDA content, decreased total SOD activity, or even apoptosis were occurred in PC12 cells when treated with CPF. Interestingly, CPF‐induced cell injury was conspicuously reversed after Ed administration. Nrf2 signaling pathway was activated after Ed treatment and the neuroprotective effect of Ed was not significant in cells after Nrf2 gene knockdown. In conclusion, Ed exerts neuroprotective effect on CPF‐induced oxidative stress injury and its mechanism was correlated with the Nrf2 signaling pathway.
Feeding strategy impacts heterologous protein production in Yarrowia lipolytica fed‐batch cultures—Insight into the role of osmolarity
Authors: Kubiak Monika , Borkowska Monika , Białas Wojciech , Korpys Paulina , Celińska Ewelina 
Published: February 2019

AbstractFed‐batch cultivation is the preferred bioprocessing strategy applied in microbial production of proteins. Feeding strategy is crucial parameters to be optimized upon development of a fed‐batch process. In this study, we investigated impact of different feeding strategies on production of recombinant enzymatic protein in Yarrowia lipolytica cultures. From amongst tested strategies, comprising intermittent and continuous feedings, also in cascade with respiratory factors, intermittent feeding executed after complete exhaustion of glycerol from the medium, with moderate amplitude of osmolarity, was the most beneficial in terms of the secretory enzyme amount, its volumetric productivity and specific activity. Because adopted feeding strategies strongly modulated osmolarity of the cultures, the effect of osmotic pressure on production of the target heterologous protein was investigated in a series of batch cultivations with addition of osmoactive compounds (NaCl, sorbitol, sucrose, and glycerol) at different concentrations. Although obvious promoting effect of the osmoactive substances on the enzyme production was clear, no straightforward correlation between the medium osmolarity and the target enzyme's specific activity could be observed. These results suggest that not only the level of osmolarity but also chemical character of the osmoactive compound have both important impact on the production of secretory proteins in Y. lipolytica cultures.
Conclusions
Authors: Wanner Philippe , Steiner Ilka 
Published: February 2019

AbstractBy confronting and assembling the main results of the previous chapters with respect to the three key dimensions – increasing mobility and transnationality, labour market participation and political and social participation – this concluding chapter discusses the implications for the Migration-Mobility Nexus framework. In addition, the authors address how these results apply to the European context and enumerate their consequences for future research. Overall, the book demonstrated how the implementation of a nationwide survey, such as the Migration-Mobility Survey, contributes to better knowledge about current migration processes and helps identify approaches and policies that can enhance immigrants’ integration when necessary. It is therefore relevant to both the scientific community and migration policy.
Two Approaches to Enhance the Processivity and Salt Tolerance of Staphylococcus aureus DNA Polymerase
Authors: Zhai Bing , Chow Joseph , Cheng Qi 
Published: February 2019

Abstract
In this article, two engineering-strategies were carried out to enhance the processivity of the DNA polymerase used in recombinase polymerase amplification (RPA). We demonstrate that covalent linkage of a non-specific, double-stranded DNA binding protein, Sso7d, to the large fragment of Staphylococcus aureus Pol I (Sau) caused a moderate enhancement of processivity and a significant improvement in the salt tolerance of Sau. Meanwhile, we provide evidence suggesting that insertion of the thioredoxin-binding domain from bacteriophage T7 DNA polymerase into the analogous position of the large fragment of Sau dramatically enhanced the processivity and mildly increased the salt tolerance of Sau when a host DNA binding protein, thioredoxin, was annexed. Both of these two strategies did not improve the amplifying performance of Sau in RPA, indicating that optimum processivity is crucial for amplifying efficiency.
Algebraic Analysis of Demodalised Analytic Implication
Authors: Ledda Antonio , Paoli Francesco , Pra Baldi Michele 
Published: February 2019

Abstract
The logic DAI of demodalised analytic implication has been introduced by J.M. Dunn (and independently investigated by R.D. Epstein) as a variation on a time-honoured logical system by C.I. Lewis’ student W.T. Parry. The main tenet underlying this logic is that no implication can be valid unless its consequent is “analytically contained” in its antecedent. DAI has been investigated both proof-theoretically and model-theoretically, but no study so far has focussed on DAI from the viewpoint of abstract algebraic logic. We provide several different algebraic semantics for DAI, showing their equivalence with the known semantics by Dunn and Epstein. We also show that DAI is algebraisable and we identify its equivalent quasivariety semantics. This class turns out to be a linguistic and axiomatic expansion of involutive bisemilattices, a subquasivariety of which forms the algebraic counterpart of Paraconsistent Weak Kleene logic (PWK). This fact sheds further light on the relationship between containment logics and logics of nonsense.
Vertical versus horizontal: What is really at issue in the exclusion problem?
Authors: Donaldson John 
Published: February 2019

AbstractI outline two ways of reading what is at issue in the exclusion problem faced by non-reductive physicalism, the “vertical” versus “horizontal”, and argue that the vertical reading is to be preferred to the horizontal. I discuss the implications: that those who have pursued solutions to the horizontal reading of the problem have taken a wrong turn.
Potential for Long-Term Benefit of Cognitive Behavioral Therapy as an Adjunct Treatment for Men with Erectile Dysfunction
Authors: Khan Sikandar , Amjad Amira , Rowland David 
Published: February 2019

Abstract

Introduction
Psychosexual counseling may enhance sexual performance outcomes in men with erectile dysfunction (ED) treated with a phosphodiesterase type 5 (PDE5) inhibitor.

Aim
To determine the potential long-term effects of cognitive behavioral therapy (CBT) on Pakistani men with ED who had undergone treatment with a PDE5 inhibitor (PDE5i).

Methods
In a 15–18-month follow-up, we reassessed a subsample of 20 men who had been treated with either PDE5is (monotherapy group) or CBT + PDE5i (combined group) on 2 dimensions: sexual functioning and mental health functioning.

Main Outcome Measure
International Index of Erectile Function was used to assess sexual function, and 2 Mental Health Inventory subscales were used to assess anxiety and depression. A brief semi-structured interview assessed men’s current sexual status and evaluation of their CBT experience.

Results
Men in the combined group continued to show improvement on erectile function and several other sexual parameters, whereas men in the monotherapy group showed either no further improvement or a decrement in sexual response parameters. The results did not appear to be related to changes in relationship satisfaction or mental health indices.

Clinical Implications
Adjunctive CBT shows long-term benefits in men with ED treated with a PDE5i.

Strength & Limitations
Effect sizes were strong, overcoming the small sample size, but attrition may affect the generalizability of the findings.

Conclusion
In the first long-term follow-up study of its kind, CBT proved an effective and supportive adjunctive treatment for Pakistani men with ED taking a PDE5i, with benefits extending long after the end of treatment.
NOTE ON THE CONVOLUTION OF HARMONIC MAPPINGS
Authors: LI LIULAN , PONNUSAMY SAMINATHAN 
Published: February 2019

Dorffet al.[‘Convolutions of harmonic convex mappings’,Complex Var. Elliptic Equ. 57(5) (2012), 489–503] formulated a question concerning the convolution of two right half-plane mappings, where the normalisation of the functions was considered incorrectly. In this paper, we reformulate the problem correctly and provide a solution to it in a more general form. We also obtain two new theorems which correct and improve related results.
ON THE DISTRIBUTION OF THE RANK STATISTIC FOR STRONGLY CONCAVE COMPOSITIONS
Authors: ZHOU NIAN HONG 
Published: February 2019

A strongly concave composition of $n$ is an integer partition with strictly decreasing and then increasing parts. In this paper we give a uniform asymptotic formula for the rank statistic of a strongly concave composition introduced by Andrews et al. [‘Modularity of the concave composition generating function’, Algebra Number Theory7(9) (2013), 2103–2139].
Human trichinellosis caused by Trichinella britovi in Greece, and literature review
Authors: Dimzas D. , Diakou A. , Koutras C. , Gómez Morales M.A. , Psalla D. , Keryttopoulos P. , Deligianni D. , Kontotasios K. , Pozio Edoardo 
Published: February 2019

Abstract
Trichinellosis is a cosmopolitan zoonotic parasitic disease caused by the nematodes of the genus Trichinella, through the consumption of raw or semi-raw infected meat from swine, horses and wild animals. This disease has been sporadically reported in Greece since 1946. The aim of the present study was to describe a trichinellosis case in a patient hospitalized in northern Greece, in 2017. A 47-year-old male was admitted to hospital with intense generalized myalgia, periorbital swelling, fever, exhaustion and anorexia. Biochemical and haematological profile showed eosinophilia and elevated creatine phosphokinase (CPK). Anti-Trichinella spp. IgG and IgM antibodies were detected by serology and Trichinella spp. larvae were found in two muscle biopsies by compressorium and histological examination. A larva collected from the muscle biopsy was identified as Trichinella britovi by polymerase chain reaction (PCR). Albendazole (400 mg twice per day × 10 days) was administered and the clinical condition of the patient promptly improved. This is the first identification of T. britovi in a patient in Greece.
Awareness of the Convention on Biological Diversity and provisions regarding access and benefit sharing among multiple stakeholder groups in Bhutan
Authors: Wang Sonam Wangyel , Lee Woo Kyun , Brooks Jeremy , Dorji Chencho 
Published: February 2019

AbstractAs part of the Convention on Biological Diversity (CBD), the Nagoya Protocol on Access and Benefit Sharing calls for ‘fair and equitable sharing of benefits’ derived from the use of genetic resources and traditional knowledge. However, implementation of the Convention and the Nagoya Protocol has been challenged by the inadequacies of existing policies, lack of national-level frameworks, and inadequate knowledge among stakeholders. We used focus group meetings and structured interviews with rural communities, government representatives, researchers and Members of Parliament in Bhutan to collect data on awareness, knowledge and perceptions of components of the CBD related to access and benefit sharing. Our study indicated generally low levels of awareness about most components of the Convention, particularly among rural residents. Although local people in rural communities feel that benefits derived from local biological resources and traditional knowledge should be shared, there is uncertainty about who owns these resources. These results indicate that there is an urgent need to develop educational and awareness programmes, using a variety of media, to target particular stakeholder groups, with emphasis on residents in rural communities. This could empower local communities to participate meaningfully in decision-making processes to develop Bhutan's national access and benefit sharing framework, and to allow them to benefit from the conservation and sustainable use of local resources.
Antiprotozoal investigation of 20 plant metabolites on Trypanosoma cruzi and Leishmania amazonensis amastigotes. Atalantoflavone alters the mitochondrial membrane potential
Authors: Lemos da Silva Layzon Antonio , Höehr de Moraes Milene , Scotti Marcus Tullius , Scotti Luciana , de Jesus Souza Rafaela , Nantchouang Ouete Judith L. , Biavatti Maique Weber , Steindel Mario , Sandjo Louis Pergaud 
Published: February 2019

AbstractThe study aims to evaluate the antiprotozoal activities of 20 plant metabolites onTrypanosoma cruziandLeishmania amazonensisamastigotes. Compounds1–20were obtained and identified by using chromatographic and spectroscopic techniques. The antiparasitic assays were performed on the intracellular form ofT. cruziandL. amazonensisusing human leukaemic THP-1 cells as the host. The mechanism of action of the most active compounds was exploredin silicoby molecular docking usingT. cruzitrypanothione reductase (TR) as a target, whereas thein vitrostudies were performed by enzymatic assay usingT. cruzirecombinant TR. In addition, the mitochondrial membrane potential was evaluated by flow cytometry. Two flavonoids, one triterpene and three acetogenins showed from high to moderate trypanocidal activities with IC50values ranging 3.6–37.2µmwhile three of the metabolites were moderately leishmanicidal. The molecular docking study revealed interactions between TR and the most trypanocidal compounds1(abyssinone IV) and2(atalantoflavone). In contrast, both showed no effect on TRin vitro. For the mitochondrial membrane potential assay, atalantoflavone (2) displayed a dose-dependent depolarization. On the basis of the aforementioned results, this compound's structure could be chemically explored in order to develop more potent trypanocidal derivatives.
Testing the cycle of maltreatment hypothesis: Meta-analytic evidence of the intergenerational transmission of child maltreatment
Authors: Madigan Sheri , Cyr Chantal , Eirich Rachel , Fearon R. M. Pasco , Ly Anh , Rash Christina , Poole Julia C. , Alink Lenneke R. A. 
Published: February 2019

AbstractIt has long been claimed that “maltreatment begets maltreatment,” that is, a parent's history of maltreatment increases the risk that his or her child will also suffer maltreatment. However, significant methodological concerns have been raised regarding evidence supporting this assertion, with some arguing that the association weakens in samples with higher methodological rigor. In the current study, the intergenerational transmission of maltreatment hypothesis is examined in 142 studies (149 samples; 227,918 dyads) that underwent a methodological quality review, as well as data extraction on a number of potential moderator variables. Results reveal a modest association of intergenerational maltreatment (k= 80;d= 0.45, 95% confidence interval; CI [0.37, 0.54]). Support for the intergenerational transmission of specific maltreatment types was also observed (neglect:k= 13,d= 0.24, 95% CI [0.11, 0.37]; physical abuse:k= 61,d= 0.41, 95% CI [0.33, 0.49]; emotional abuse:k= 18,d= 0.57, 95% CI [0.43, 0.71]; sexual abuse:k= 18,d= 0.39, 95% CI [0.24, 0.55]). Methodological quality only emerged as a significant moderator of the intergenerational transmission of physical abuse, with a weakening of effect sizes as methodological rigor increased. Evidence from this meta-analysis confirms the cycle of maltreatment hypothesis, although effect sizes were modest. Future research should focus on deepening understanding of mechanisms of transmission, as well as identifying protective factors that can effectively break the cycle of maltreatment.
Factors associated with the availability of iodized salt at household level: a case study in Bangladesh
Authors: Khan Jahidur Rahman , Biswas Raaj Kishore , Sheikh Md Tuhin , Huq Molla 
Published: February 2019

AbstractObjectiveThe availability of iodized salt in households remains low in Bangladesh, which calls for improving the salt iodization quality and its coverage. The present study assessed the socio-economic disparity in Bangladesh to characterize the availability of iodized salt at household level.DesignAssociations between different socio-economic factors and availability of iodized salt at household level were explored using Bayesian mixed-effects logistic models after adjusting the district- and cluster-level random effects.SettingBangladesh Multiple Indicator Cluster Survey (MICS), 2012–13.ParticipantsHouseholds (sample size,n50981).ResultsResults showed that 73·15 % of household salt samples were iodized to some extent although iodization level varied. According to the regression model, houses with young (adjusted odds ratio of posterior mean (OR) = 1·31; 95 % credible interval (CI) 1·09, 1·64) and educated (OR = 3·66; 95 % CI 3·25, 4·23) household heads had significantly higher likelihood of availability of iodized salt. In addition, iodized salt was less likely be found in poor and rural households, as urban households were 2·88 times (95 % CI 2·41, 3·34) more likely have iodized salt. Moreover, the regional locations of the households were an important component that contributed to the local iodized salt coverage. As per the district-wise distribution, the north-west part of Bangladesh and Cox’s Bazar in the far south seemed to lack household-level iodized salt.ConclusionsOur findings suggest that iodized salt intervention should be promoted considering the area variations, which could potentially help policy makers to design interventions in the context of Bangladesh.
Automatic Counting of Intra-Cellular Ribonucleo-Protein Aggregates inSaccharomyces cerevisiaeUsing a Textural Approach
Authors: Marin Ambroise , Denimal Emmanuel , Bertheau Lucie , Guyot Stéphane , Journaux Ludovic , Molin Paul 
Published: February 2019

AbstractIn the context of microbiology, recent studies show the importance of ribonucleo-protein aggregates (RNPs) for the understanding of mechanisms involved in cell responses to specific environmental conditions. The assembly and disassembly of aggregates is a dynamic process, the characterization of the stage of their evolution can be performed by the evaluation of their number. The aim of this study is to propose a method to automatically determine the count of RNPs. We show that the determination of a precise count is an issue by itself and hence, we propose three textural approaches: a classical point of view using Haralick features, a frequency point of view with generalized Fourier descriptors, and a structural point of view with Zernike moment descriptors (ZMD). These parameters are then used as inputs for a supervised classification in order to determine the most relevant. An experiment using a specificSaccharomyces cerevisiaestrain presenting a fusion between a protein found in RNPs (PAB1) and the green fluorescent protein was performed to benchmark this approach. The fluorescence was observed with two-photon fluorescence microscopy. Results show that the textural approach, by mixing ZMD with Haralick features, allows for the characterization of the number of RNPs.