bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lotman’s semiotic theory of culture or Laclau’s political ontology?
Authors: Mihkelsaar Janar 
Published: August 2018

AbstractThe present article concentrates on the main discrepancies that should arise in the discussion between Lotman’s semiotics of culture and Laclau’s discursive theory of hegemony. Some significant – but still abstract – commonalities conceal fundamental disagreements which I would group around four topics. Firstly, Lotman’s semiotic method is at odds with Laclau’s ontological way of thinking. Secondly, although both Lotman and Laclau subscribe to the openness of signification, it is impossible to incorporate their accounts of this openness without loose ends. In order to substantiate this claim, I examine Lotman’s concept of “boundary” and Laclau’s concept of the “limit.” Thirdly, we should avoid reading too much into Lotman and Laclau’s agreement on the similar – but still formal – model of a self-signification. And finally, Laclau’s valorization of social antagonism is in conflict with Lotman’s appraisal of dialogue. Confronted with these discrepancies, we are enforced to decide whether to endorse Lotman’s cultural semiotics or Laclau’s political ontology.
A new method to quantify atmospheric Poaceae pollen DNA based on the trnT-F cpDNA region
Authors: Alan Şenol , Sarışahin Tuğba , Şahin Aydan Acar , Kaplan Ayşe , Erdoğan İbrahim , Pınar Nur Munevver 
Published: August 2018

Abstract

Background
Pollen, mold spores, bacteria and viruses are the main biological substances in the atmosphere causing allergic symptoms and disease. Distinguishing pollen and spores is quite time consuming and requires a trained expert. There is a different approach to identification of these substances such as microscopic analysis. However, DNA based identification of these is becoming popular recently.

Objective
We evaluated the correlation between the quantity of DNA, which was amplified using trnT-F cpDNA specific primers in samples obtained from a high volume air sampler (HVAS), and concentration of Poaceae pollen collected with a Burkard trap.

Materials and methods
Here, we present a method for identifying and quantifying airborne Poaceae pollen using a single step polymerase chain reaction (PCR) technique. Forty daily air samples were collected by HVAS. The method was optimised using two different methods (M1 and M2) and the trnT-F cpDNA region was amplified using a Poaceae specific primer pair. The correlation between the quantity of DNA and pollen concentration was tested using R statistical programming language.

Results
Although a significant correlation was obtained between the M1 and M2 methods (R2=0.655, p<0.01), the M2 method was more correlated with pollen concentration. The correlation between pollen and DNA content changed due to episodes that were observed during the pollen season. DNA concentrations from the PCR data were significantly correlated with pollen concentrations determined by light microscopy (R2=0.767, p<0.01) in episode II using the M2 method and during the entire season (R2=0.469, p<0.01) using M2.

Conclusions
The M2 method correctly identified Poaceae pollen in mixed air samples from Zonguldak Province. The non-coding trnT-F cpDNA region was used for the first time in aerobiological samples to identify Poaceae pollen. Use of this method that does not require DNA extraction may be a crucial step for real-time pollen monitoring devices to be developed in the future. The correlation strength between pollen and amplified DNA content could be improved using a sampler that has a lower absorption rate, and a more sensitive technique, such as qPCR.
Identification of cytoplasmic sialidase NEU2-associated proteins by LC-MS/MS
Authors: Akyildiz Demir Secil , Seyrantepe Volkan 
Published: August 2018

Abstract

Background
Cytoplasmic sialidase (NEU2) plays an active role in removing sialic acids from oligosaccharides, glycopeptides, and gangliosides in mammalian cells. NEU2 is involved in various cellular events, including cancer metabolism, neuronal and myoblast differentiation, proliferation, and hypertrophy. However, NEU2-interacting protein(s) within the cell have not been identified yet.

Objective
The aim of this study is to investigate NEU2 interacting proteins using two-step affinity purification (TAP) strategy combined with mass spectrometry analysis.

Methods
In this study, NEU2 gene was cloned into the pCTAP expression vector and transiently transfected to COS-7 cells by using PEI. The most efficient expression time of NEU2- tag protein was determined by real-time PCR and Western blot analysis. NEU2-interacting protein(s) were investigated by using TAP strategy combined with two different mass spectrometry experiment; LC-MS/MS and MALDI TOF/TOF.

Results
Here, mass spectrometry analysis showed four proteins; α-actin, β-actin, calmodulin and histone H1.2 proteins are associated with NEU2. The interactions between NEU2 and actin filaments were verified by Western blot analysis and immunofluorescence analysis.

Conclusions
Our study suggests that association of NEU2 with actin filaments and other protein(s) could be important for understanding the biological role of NEU2 in mammalian cells.
Maximal B-factors in protein crystal structures
Authors: Carugo Oliviero 
Published: August 2018

Abstract
Since the contribution of proteins atoms with exceptionally large B-factors, much larger than those of other atoms, to the observed structure factors is negligible, it is therefore recommended to consider with a care the parts of the models where B-factors are inflated to extremely large values. Otherwise, molecular biologists risk to over-interpret structural data, since the positions of these atoms is highly uncertain. In the present communication, maximal B-factor values, over which the atoms can be considered ‘invisible’, are estimated based on the relationships between B-factors and solvent accessible surface areas.
Studies on Sputtered Cu–Zn–Sn–O Precursor to Fabricate Cu2ZnSnS4 Thin Films
Authors: Liu Xinxing , Hao Ruiting , Zhao Qichen , Chang Faran , Li Yong , Gu Kang , Wang Lu , Liu Bin , Guo Jie 
Published: August 2018

Abstract
The Cu2ZnSnS4 (CZTS) thin films were fabricated by sulfurization of radiofrequency magnetron sputtered Cu–Zn–Sn–O (CZTO) precursors. Here, we extend recent works in the field of fabricating CZTO precursors by a new approach sputtering ZnO/Sn/Cu targets. The effects of one-step and two-step annealing processes applied for CZTO precursors on the structure, morphology, optical, and electrical properties were investigated systematically. The preannealing step of fundamental phase formation in the sulfurization process was also discussed. The two-step annealing process was found to affect the composition of element Sn slightly but significantly improved crystallinity, CZTS/Mo interfacial conditions, surface roughness, and electrical properties. The two-step annealed CZTS thin films had excellent optical and electrical properties with an optical band gap of 1.51 eV, a hole concentration of 2.4 × 1017 cm−3, and a hole mobility of 1.97 cm2/(V⋅s). In addition, the CZTS/Mo interface with small grains and voids were significantly improved. CZTS-based solar cell devices were successfully fabricated. The characteristics of current–voltage (J–V) curves indicated that short-circuit currents had a tendency to increase with the improvement of CZTS/Mo interface and surface morphology. As a result, the device based on two-step annealed CZTS thin films exhibited better performance with an open-circuit voltage of 553 mV, short-circuit current of 7.2 mA⋅cm−2, a fill factor of 37.8%, and a conversion efficiency of 1.51%.
The Dorsal Compound Eye of Simuliid Flies:
Authors: Kirschfeld K. , Wenk P. 
Published: August 2018

Abstract
Compound Eye, Light-Quantum Noise The highly specialized dorsal compound eye of male Simuliids is especially adapted to detect during flight the females which are very small animals (1×3 mm). At dawn, the eye functions close to the physical limit imposed by the quantum fluctuations of light. In bright illuminations, light-qauntum noise is not a limiting factor as can be shown by behavioural experiments.
Carotenoids, tocopherols, organic acids, charbohydrate and mineral content in different medicinal plant extracts
Authors: Mihaylova Dasha , Vrancheva Radka , Petkova Nadezhda , Ognyanov Manol , Desseva Ivelina , Ivanov Ivan , Popova Mariya , Popova Aneta 
Published: August 2018

AbstractNettle (Urtica dioicaL.), tansy (Tanacetum vulgareL.), bladder campion (Silene vulgaris(Moench) Garcke, waterpepper (Polygonum hydropiperL.), common centaury (Centaurium erythraeaPers.) and rose hip fruit (Rosa caninaL. cv. Plovdiv 1) were used for preparation of different water extracts (infusion, decoction and microwave extract) and ethanol (tincture) extracts. Carotenoids (lutein, lycopene and β-carotene), tocopherols (α-, γ- and δ-), organic acids (ascorbic, malic, fumaric and citric), five macro- and three microelements, sugars and uronic acids content in the obtained extracts were analyzed. Among the investigated plants, stinging nettle, bladder campion and rose hip fruit were evaluated as most potential with respect to bioactive compounds and microelements. The results showed that the selected six medicinal plants and their extracts can be presented as sources of dietary fibers and micronutrients, which may encourage further application as food supplements and beverages as well as to motivate plant use as a dietary alternative in different foods. The present study is a first detailed analysis with respect to sugar content of decoction, infusion and tincture ofS. vulgaris.
Monitoring Blood Biomarkers and Training Load Throughout a Collegiate Soccer Season
Authors: Huggins Robert A. , Fortunati Andrea R. , Curtis Ryan M. , Looney David P. , West Chris A. , Lee Elaine C. , Fragala Maren S. , Hall Matthew L. , Casa Douglas J. 
Published: August 2018

Abstract
Huggins, RA, Fortunati, AR, Curtis, RM, Looney, DP, West, CA, Lee, EC, Fragala, MS, Hall, ML, and Casa, DJ. Monitoring blood biomarkers and training load throughout a collegiate soccer season. J Strength Cond Res 33(11): 3065–3077, 2019—This observational study aimed to characterize the responses of a comprehensive panel of biomarkers, observed ranges, training load (TL) metrics, and performance throughout the collegiate soccer season (August–November). Biomarkers (n = 92) were collected before the start of pre-season (PS), in-season weeks (W)1, W4, W8, and W12 in NCAA Division I male soccer players (n = 20, mean ± SD; age = 21 ± 1 years, height = 180 ± 6 cm, body mass = 78.19 ± 6.3 kg, body fat = 12.0 ± 2.6%, Vo
2max 51.5 ± 5.1 ml·kg·min−1). Fitness tests were measured at PS, and W12 and TL was monitored daily. Changes in biomarkers and performance were calculated via separate repeated-measures analysis of variance. Despite similar fitness (p > 0.05), endocrine, muscle, inflammatory, and immune markers changed over time (p < 0.05). Total and free testosterone was lower in W1 vs. PS, whereas free cortisol remained unchanged at PS, W1, and W4 (>0.94 mg·dL−1). Oxygen transport and iron metabolism markers remained unchanged except for HCT (W1 vs. PS) and total iron binding capacity (W8–W12 vs. W1). Hepatic markers albumin, globulin, albumin:globulin, and total protein levels were elevated (p < 0.05) at W12 vs. W1, whereas aspartate aminotransferase and alanine aminotransferase levels were elevated at W1–W12 and W8–W12 vs. PS, respectively. Vitamin E, zinc, selenium, and calcium levels were elevated (p < 0.05) at W12 vs. W1, whereas Vitamin D was decreased (p < 0.05). Fatty acids and cardiovascular markers (omega-3 index, cholesterol:high-density lipoprotein [HDL], docosahexenoic acid, low-density lipoprotein [LDL], direct LDL, non-HDL, ApoB) were reduced at W1 vs. PS (p ≤ 0.05). Immune, lipid, and muscle damage biomarkers were frequently outside clinical reference ranges. Routine biomarker monitoring revealed subclinical and clinical changes, suggesting soccer-specific reference ranges. Biomarker monitoring may augment positive adaptation and reduce injuries from stressors incurred during soccer.
Characterizing the Temperature Effects on Rutting and Fatigue Properties of Asphalt Binders Based on Time-Temperature Superposition Principle
Authors: Wang Chao , Wang Yang , Chen Yangzhou , Diab Aboelkasim , You Zhanping 
Published: August 2018

Abstract
The objective of this article is to investigate the effects of temperature on rutting and fatigue cracking resistance of asphalt binders based on the time-temperature superposition principle (TTSP). In this study, the TTSP approach was employed to interpret the results of multiple stress creep recovery (MSCR) and linear amplitude sweep (LAS) tests for both neat and styrene-butadiene-styrene (SBS)–modified asphalt binders. The higher stress level of 3.2 kPa in the standard MSCR test procedure was extended to 10 and 15 kPa to verify the stress sensitivity of the nonrecoverable compliance (Jnr). A loading duration of 5 minutes in the standard LAS test was extended to 10 and 15 minutes to develop the fatigue failure criterion at different conditions. The results demonstrate that the time-strain curves of the neat binder during the MSCR tests under different temperatures can be unified into a single strain master curve using the TTSP shift factors, based on which the predictive Jnr showed good agreement with the measured values. This TTSP-based analysis approach does not work well for the MSCR results of the SBS-modified binder at high temperatures. However, the fatigue failure criterion determined from the LAS tests under different temperatures was verified to be successfully constructed into a single failure criterion according to the TTSP approach for both the neat and SBS-modified binders. Therefore, only an additional caution is raised for the TTS-based analysis on rutting resistance of the modified binder in a high temperature range.
Multiscale Experiments and Multifractal Analysis of Bituminous Coals: Dominant Pore Properties for Gas Permeability
Authors: Zhang Bo , Zhu Jie , Jiang Yaodong 
Published: August 2018

Abstract
The pore characteristics of coal are highly correlated with gas permeability. In this study, multifractal analysis based on mercury intrusion porosimetry (MIP) was used to investigate the pore spatial distributions and heterogeneity of pore size distributions (PSDs) of three sister coal samples collected from the Xin Zhouyao mine in China. The mercury intrusion data, under pressures of over 10 MPa, were corrected in combination with the gas adsorption results. All CT images were processed by using the Image-Pro Plus® (IPP) software (Media Cybernetics, Inc., Rockville, MD). The pore volume percentage information obtained from the MIP was found to be coincident with the 3D reconstructions. Seepage experiments were also carried out under different gas pressures to analyze the effect of the pore parameters on permeability. The results from these experiments showed that for the three coal samples, the degree of clustering of the pores is the dominant factor for permeability and should be the first factor considered. Variations of μ (q, ϵ) versus ϵ indicated that the PSDs of coals have multifractal behavior. Multifractal analysis was suggested by the extracted parameters from the PSDs, including the capacity dimension D0, the information dimension D1, the correlation dimension D2, the widths of the right side D0–D10, and of the left side D−10–D0 of Dq spectra, and the widths of left side α0–αq+ and right side α0–αq− of the f (α) spectrum. These parameters were further discussed to evaluate the permeability.
EL SUICIDIO EN LA HOGUERA. CAMUS Y LA PENA CAPITAL
Authors: Trujillo Iván 
Published: August 2018

<pre>Es conocida la oposición de Albert Camus a la pena capital. En sus Réflexions sur la guillotine (1957) va a criticar la máquina judicial que hace sufrir en el tiempo y en el espacio al condenado a muerte. Antes, en L'homme revolté (1951), ha criticado la perversa lógica contractual rousseauniana implicada en la auto-imputación que conduce silenciosamente a Saint-Just al patíbulo en la época del terror revolucionario en Francia y a los condenados a muerte en la purgas del régimen de Stalin en la Unión Soviética. Pero unos años antes, en El Mito de Sísifo (1942), hablando del suicidio, se ha referido a Galileo y al peligro que ha corrido de morir en la hoguera por defender sus verdades. Nos preguntamos aquí por aquello que, en el pensamiento de Camus, convierte a la pena capital en suicidio. <br />
<br />
</pre>
A lakóhelytől függ az esély a jobb minőségű ellátásra – Az ellátáshoz való hozzáférés területi egyenlőtlenségei Magyarországon, a szürkehályogműtétek példáján
Authors: Uzzoli Annamária , Vitrai József , Bakacs Márta , Gémes Katalin , Kiss Norbert , Kövi Rita 
Published: August 2018

Az egészségi állapotban jelen lévő egyenlőtlenségek csökkentésének egyik hatékony eszköze az egészségügyi szolgáltatásokhoz való hozzáférés feltételeinek és a hozzáférés esélyegyenlőségének biztosítása. Az egészségügyi ellátáshoz való hozzáférés vizsgálatában a közfinanszírozott szürkehályogműtétek 2008. évi adatait vizsgáltuk. A kutatás során arra voltunk kíváncsiak, hogyan oszlanak meg az ellátási kapacitások és az ellátási típusok a szolgáltatást végző intézmények között, illetve milyen domináns útvonalak különíthetők el az igénybevételhez kapcsolódó betegmobilitásban. Regionális és kistérségi esettanulmányunkban külön elemeztük, hogy egyrészt az egészségügyi intézmények elérhetősége milyen mértékben van hatással az igénybevételre, másrészt mekkora különbség tapasztalható az utazási távolságban, időtartamban és költségekben akkor, amikor a beteg nem a lakóhelyéhez legközelebbi intézményben vette igénybe a műtéti szolgáltatást. A lakóhelytől függően nagy egyenlőtlenséget tapasztaltunk az intézmények elérhetőségében. A műtétek során a betegek nagy része nem a lakóhelyéhez legközelebbi intézményt kereste fel, ezzel vállalva az utazási és kiadási többletet. Közülük sokan a területi ellátási kötelezettség alapján, tehát a szabályokat követve utaztak a távolabbi ellátó intézménybe. A lakosság egészségügyi ellátással való elégedettségének növelésében a jövőben azt is vizsgálni kell, hogy milyen további tényezők befolyásolják a betegek orvos- és intézményválasztását.A tanulmány javaslatokat fogalmaz meg a térségi egészségügyi ellátórendszerek kialakításához, valamint kísérletet tesz egy olyan módszertan kidolgozására, amely segítségül szolgálhat a szakpolitikai döntéshozatalhoz.
Kopács mint az emlékezet helye és az emlékezetre ható jelek a térben
Authors: Baumann Tímea 
Published: August 2018

A tanulmány arra igyekszik rámutatni, hogy az emlékezet helyeinek és terének elemzése milyen jelentőségű lehet egy emlékezetközösség vizsgálatának szempontjából. Ennek céljából a Kopácson (Kopačevo) élő horvátországi magyar közösséget mint emlékezetközösséget veszi vizsgálat alá, melyet a délszláv háborúra vonatkozó traumatikus emlékek és ezek elbeszélési formái határoznak meg – ezek az emlékek formálják egy közösséggé a Kopácson élőket és azokat, akik menekültként eltávoztak a faluból. Jelen tanulmány azokat az elbeszélési és felejtési kísérleteket mutatja be, amelyek a térbeliségben öltenek testet.Miután a tanulmány rávilágít a tér és a helyek jelenlétére és szerepére az emlékezetről szóló társadalomtudományi írásokban – kiemelve Pierre Nora munkáit és a lieu de mémoire fogalmát –, Kopácsot mint az emlékezet terét tárja az olvasó elé. A falut a délszláv háború idején a Jugoszláv Néphadsereg szállta meg, majd a terület a nemzetközileg el nem ismert Krajinai Szerb Köztársaság részévé vált. Ezen események hatására a kopácsi magyar közösség három részre szakadt: 1. otthonmaradókra, akik a faluban maradtak és szerb megszállás alatt éltek; 2. külföldre (elsősorban Magyarországra) menekültekre; 3. Eszékre (Horvátországba) menekültekre. A tanulmány e három csoportnak Kopácshoz mint az emlékezet teréhez fűződő emlékeit elemzi.A faluban található emlékezethelyek közül a tanulmány egyrészt emlékműveket és hozzájuk kapcsolódó szertartásokat, másrészt olyan helyeket vizsgál, amelyek hatnak a közösség emlékezetére. Bemutatja a falubeli délszláv háborús áldozatok emléktábláját. A táblát 2002. december 3-án avatták fel, mely dátum a lokális naptárban a délszláv háborúra irányítja a figyelmet, mivel ezen a napon egy a faluból származó katonát öltek meg a szerbek Kopács határában. Az emlékezetre ható jelként mutatja be a tanulmány az első világháborús emlékművet, amely egy katonát jelenít meg. A szobor arcát a délszláv háború során a faluban állomásozó szerb katonák szétlőtték. A szétroncsolt arc így a falubeliek szenvedésének szimbólumává vált, egészen addig, amíg úgy nem döntöttek, hogy ezt a fájó emlékeztető jelet (és egyben az általa megjelenített traumát) egy új arccal fedik le.A tanulmány második fele azokkal a térbeli tényezőkkel foglalkozik, amelyek az emlékezetközösség tagjainak narratíváiban jelennek meg. Tehát az írás amellett érvel, hogy a narratív interjú módszerével is vizsgálható a térbeliség. Végül pedig az elemzett szövegekhez kapcsolva mentális térképeket is bemutat a szerző.
Significance of biomechanical indices assessment in orthopedic correction of motor disorders in patients following cerebral stroke
Authors: Schekolova N. B. , Bronnikov V. A. , Ladeischikov V. M. , Zinoviev A. M. 
Published: August 2018

Aim. To study the biomechanical peculiarities of orthopedic disorders in patients after cerebral stroke and estimate possibilities for their correction.
Materials and methods. One hundred and thirty two persons in the late rehabilitation period after the cerebral stroke were examined. The state of loco-motor system biomechanics was assessed, using the rehabilitation complexes “TRUST-M” and “ST-150”.
Results. The late rehabilitation period of stroke was characterized by decrease in walking rate and time, rise in step cycle. Speed of movement was disturbed. Combined pain, spasticity, contracture and functional asymmetry of the extremities length determined the formation of individual variant of patient’s pathological locomotor stereotype. When implementing correlation analysis, marked correlation between the severity of orthopedic disorders and the asymmetry of pelvis and extremities length was detected. To correct biomechanical disorders, it was recommended to use complex orthopedic treatment by means of prosthetic-orthopedic devices, which permitted to move and provided stable holding of extremity segments in the desired position.
Conclusion. 1. Biomechanical indices characterize the severity of orthopedic pathology in the late rehabilitation period following cerebral stroke. 2. Biomechanical disorders are connected with decreased walking rate and time, raised step cycle, impaired speed of movement. 3. Timely biomechanical orthopedic diagnosis and adequacy of applying prosthetic-orthopedic devices guarantee efficiency of rehabilitation measures in patients after cerebral stroke.