bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cellular dissection of malaria parasite invasion of human erythrocytes using viable Plasmodium knowlesi merozoites
Authors: Lyth Oliver , Vizcay-Barrena Gema , Wright Katherine E. , Haase Silvia , Mohring Franziska , Najer Adrian , Henshall Isabelle G. , Ashdown George W. , Bannister Lawrence H. , Drew Damien R. , Beeson James G. , Fleck Roland A. , Moon Robert W. , Wilson Danny W. , Baum Jake 
Published: June 2018

AbstractPlasmodium knowlesi, a zoonotic parasite causing severe-to-lethal malaria disease in humans, has only recently been adapted to continuous culture with human red blood cells (RBCs). In comparison with the most virulent human malaria, Plasmodium falciparum, there are, however, few cellular tools available to study its biology, in particular direct investigation of RBC invasion by blood-stage P. knowlesi merozoites. This leaves our current understanding of biological differences across pathogenic Plasmodium spp. incomplete. Here, we report a robust method for isolating viable and invasive P. knowlesi merozoites to high purity and yield. Using this approach, we present detailed comparative dissection of merozoite invasion (using a variety of microscopy platforms) and direct assessment of kinetic differences between knowlesi and falciparum merozoites. We go on to assess the inhibitory potential of molecules targeting discrete steps of invasion in either species via a quantitative invasion inhibition assay, identifying a class of polysulfonate polymer able to efficiently inhibit invasion in both, providing a foundation for pan-Plasmodium merozoite inhibitor development. Given the close evolutionary relationship between P. knowlesi and P. vivax, the second leading cause of malaria-related morbidity, this study paves the way for inter-specific dissection of invasion by all three major pathogenic malaria species.
Large-scale production of megakaryocytes in microcarrier-supported stirred suspension bioreactors
Authors: Eicke Dorothee , Baigger Anja , Schulze Kai , Latham Sharissa L. , Halloin Caroline , Zweigerdt Robert , Guzman Carlos A. , Blasczyk Rainer , Figueiredo Constança 
Published: June 2018

AbstractMegakaryocytes (MKs) are the precursors of platelets (PLTs) and may be used for PLT production in vivo or in vitro, as well as a source for PLT-derived growth factors. Induced pluripotent stem cells represent an unlimited cell source for the in vitro production of MKs. This study aimed at developing an effective, xeno-free and scalable system to produce high numbers of MKs. In particular, microcarrier beads-assisted stirred bioreactors were evaluated as a means of improving MK yields. This method resulted in the production of 18.7 × 107 MKs per 50 ml medium. Laminin-coated microcarriers increased MK production per iPSC by up to 10-fold. MKs obtained in this system showed typical features of mature MKs and were able to produce PLTs in vitro and in vivo. To increase safety, MKs produced in the bioreactors were irradiated; a procedure that did not affect their capability to form proPLTs and PTLs after transfusion. In vitro generated MKs represent a promising alternative to donor PLTs and open the possibility for the development of innovative MK-based cell therapies.
Modeling the distributions of tegu lizards in native and potential invasive ranges
Authors: Jarnevich Catherine S. , Hayes Mark A. , Fitzgerald Lee A. , Yackel Adams Amy A. , Falk Bryan G. , Collier Michelle A. M. , Bonewell Lea’ R. , Klug Page E. , Naretto Sergio , Reed Robert N. 
Published: June 2018

AbstractInvasive reptilian predators can have substantial impacts on native species and ecosystems. Tegu lizards are widely distributed in South America east of the Andes, and are popular in the international live animal trade. Two species are established in Florida (U.S.A.) - Salvator merianae (Argentine black and white tegu) and Tupinambis teguixin sensu lato (gold tegu) – and a third has been recorded there— S. rufescens (red tegu). We built species distribution models (SDMs) using 5 approaches (logistic regression, multivariate adaptive regression splines, boosted regression trees, random forest, and maximum entropy) based on data from the native ranges. We then projected these models to North America to develop hypotheses for potential tegu distributions. Our results suggest that much of the southern United States and northern México probably contains suitable habitat for one or more of these tegu species. Salvator rufescens had higher habitat suitability in semi-arid areas, whereas S. merianae and T. teguixin had higher habitat suitability in more mesic areas. We propose that Florida is not the only state where these taxa could become established, and that early detection and rapid response programs targeting tegu lizards in potentially suitable habitat elsewhere in North America could help prevent establishment and abate negative impacts on native ecosystems.
The frontal skull Hounsfield unit value can predict ventricular enlargement in patients with subarachnoid haemorrhage
Authors: Won Yu Deok , Na Min Kyun , Kim Choong Hyun , Kim Jae Min , Cheong Jin Hwan , Ryu Je Il , Han Myung-Hoon 
Published: June 2018

AbstractHydrocephalus is a common complication following subarachnoid haemorrhage (SAH) arising from spontaneous aneurysm rupture. The Hounsfield unit (HU) value from computed tomography scans may reflect bone mineral density, which correlates with body mass index, which in turn is related to post-SAH ventricle size changes. We herein investigated potential associations between frontal skull HU values and ventricle size changes after SAH. HU values from four different areas in the frontal bone were averaged to minimize measurement errors. The bicaudate index and Evans ratio were measured using both baseline and follow-up CT images. CT images with bicaudate index >0.2 and Evans ratio >0.3 simultaneously were defined as indicating ventriculomegaly. We included 232 consecutive patients with SAH due to primary spontaneous aneurysm rupture, who underwent clipping over almost a 9-year period at a single institution. The first tertile of frontal skull HU values in older patients (≥55 years) was an independent predictor of ventriculomegaly after SAH, as compared to the third tertile in younger patients (hazard ratio, 4.01; 95% confidence interval 1.21–13.30; p = 0.023). The lower frontal skull HU value independently predicted ventricular enlargement post-SAH, due to the potential weak integrity of subarachnoid trabecular structures in younger patients.
Repetitive transcranial magnetic stimulation reveals a causal role of the human precuneus in spatial updating
Authors: Müller Notger G. , Riemer Martin , Brandt Lisa , Wolbers Thomas 
Published: June 2018

AbstractAs we move through an environment, the positions of surrounding objects relative to our body constantly change, with some objects even leaving our field of view. As a consequence, maintaining orientation requires spatial updating, the continuous monitoring of self-motion cues to update external locations within an egocentric frame of reference. While previous research using functional magnetic resonance imaging has implicated the precuneus in spatial updating, direct evidence for this claim is missing. To address this important question, we applied theta burst repetitive transcranial magnetic stimulation (rTMS) over the precuneus to induce a “virtual lesion”. Following stimulation, participants were tested in a large-scale virtual environment in which they had to use visual self-motion information to keep track of the position of virtual objects. Compared to sham stimulation, rTMS affected working memory traces for object locations. Critically, rTMS further impaired the ability to update these locations whenever participants experienced simulated movement. As this effect could not be explained by working memory deficits alone, we conclude that visual spatial updating relies on the construction of updated representations of egocentric object locations within the precuneus. Together, these findings establish the precuneus as performing key computations for the formation of cognitive maps.
The small molecule drug diminazene aceturate inhibits liver injury and biliary fibrosis in mice
Authors: Rajapaksha Indu G. , Mak Kai Y. , Huang Ping , Burrell Louise M. , Angus Peter W. , Herath Chandana B. 
Published: June 2018

AbstractThere is no established medical therapy to treat biliary fibrosis resulting from chronic inflammation in the biliary tree. We have recently shown that liver-specific over-expression of angiotensin converting enzyme 2 (ACE2) of the renin angiotensin system (RAS) ameliorated liver fibrosis in mice. Diminazene aceturate (DIZE), a small molecule drug approved by the US Food and Drug Administration, which is used to treat human trypanosomiasis, has been shown to have antifibrotic properties by enhancing ACE2 activity. In this study we sought to determine the therapeutic potential of DIZE in biliary fibrosis using bile duct ligated and multiple drug resistant gene-2 knockout mice. Additionally, human hepatic stellate (LX-2) and mouse Kupffer (KUP5) cell lines were used to delineate intracellular pathways. DIZE treatment, both in vivo and in vitro, markedly inhibited the activation of fibroblastic stellate cells which was associated with a reduced activation of Kupffer cells. Moreover, DIZE-inhibited NOX enzyme assembly and ROS generation, activation of profibrotic transcription factors including p38, Erk1/2 and Smad2/3 proteins and proinflammatory and profibrotic cytokine release. These changes led to a major reduction in biliary fibrosis in both models without affecting liver ACE2 activity. We conclude that DIZE has a potential to treat biliary fibrosis.
Tissue and exosomal miRNA editing in Non-Small Cell Lung Cancer
Authors: Nigita Giovanni , Distefano Rosario , Veneziano Dario , Romano Giulia , Rahman Mohammad , Wang Kai , Pass Harvey , Croce Carlo M. , Acunzo Mario , Nana-Sinkam Patrick 
Published: June 2018

AbstractRNA editing in microRNAs has been recently proposed as a novel biomarker in cancer. Here, we investigated RNA editing by leveraging small-RNA sequencing data from 87 NSCLC (Non-Small Cell Lung Cancer) samples paired with normal lung tissues from The Cancer Genome Atlas (TCGA) combined with 26 plasma-derived exosome samples from an independent cohort. Using both the editing levels and microRNA editing expression, we detected deregulated microRNA editing events between NSCLC tumor and normal tissues. Interestingly, and for the first time, we also detected editing sites in the microRNA cargo of circulating exosomes, providing the potential to non-invasively discriminate between normal and tumor samples. Of note, miR-411-5p edited in position 5 was significantly dysregulated in tissues as well as in exosomes of NSCLC patients, suggesting a potential targetome shift relevant to lung cancer biology.
Dietary methionine deficiency affects oxidative status, mitochondrial integrity and mitophagy in the liver of rainbow trout (Oncorhynchus mykiss)
Authors: Séité Sarah , Mourier Arnaud , Camougrand Nadine , Salin Bénédicte , Figueiredo-Silva A. Cláudia , Fontagné-Dicharry Stéphanie , Panserat Stéphane , Seiliez Iban 
Published: June 2018

AbstractThe low levels of methionine in vegetable raw materials represent a limit to their use in aquafeed. Methionine is considered as an important factor in the control of oxidative status. However, restriction of dietary methionine has been shown to reduce generation of mitochondrial oxygen radicals and thus oxidative damage in liver. Here, we aim to evaluate the effect of dietary methionine deficiency in hepatic oxidative status in rainbow trout and identify the underlying mechanisms. Fish were fed for 6 weeks diets containing two different methionine concentrations: deficient (MD, Methionine Deficient diet) or adequate (CTL, control diet). At the end of the experiment, fish fed the MD diet showed a significantly lower body weight and feed efficiency compared to fish fed the CTL diet. Growth reduction of the MD group was associated to a general mitochondrial defect and a concomitant decrease of the oxidative status in the liver. The obtained results also revealed a sharp increase of mitochondrial degradation through mitophagy in these conditions and emphasized the involvement of the PINK1/PARKIN axis in this event. Collectively, these results provide a broader understanding of the mechanisms at play in the reduction of oxidant status upon dietary methionine deficiency.
Low-Frequency Raman Spectroscopy of Few-Layer 2H-SnS2
Authors: Sriv Tharith , Kim Kangwon , Cheong Hyeonsik 
Published: June 2018

AbstractWe investigated interlayer phonon modes of mechanically exfoliated few-layer 2H-SnS2 samples by using room temperature low-frequency micro-Raman spectroscopy. Raman measurements were performed using laser wavelengths of 441.6, 514.4, 532 and 632.8 nm with power below 100 μW and inside a vacuum chamber to avoid photo-oxidation. The intralayer Eg and A1g modes are observed at ~206 cm−1 and 314 cm−1, respectively, but the Eg mode is much weaker for all excitation energies. The A1g mode exhibits strong resonant enhancement for the 532 nm (2.33 eV) laser. In the low-frequency region, interlayer vibrational modes of shear and breathing modes are observed. These modes show characteristic dependence on the number of layers. The strengths of the interlayer interactions are estimated by fitting the interlayer mode frequencies using the linear chain model and are found to be 1.64 × 1019 N · m−3 and 5.03 × 1019 N · m−3 for the shear and breathing modes, respectively.
Fluidically Switchable Metasurface for Wide Spectrum Absorption
Authors: Ghosh Saptarshi , Lim Sungjoon 
Published: June 2018

AbstractMetasurfaces, owing to their attractive features, provide a wide range of potential applications. Electromagnetic absorbers based on metasurfaces have significantly improved responses compared to the earlier absorbers made from composite materials. Active metasurfaces, in contrast to the passive designs, can exhibit multifunctional characteristics without repeated fabrication. This paper presents a fluidically-reconfigurable active metasurface that provides switchable wide spectrum absorption. The proposed design is comprised of liquid-metal-encased dielectric substrates, sandwiched between the top resistive pattern and bottom ground plane. With precise control of the liquid metal flow, the structure can exhibit wide absorption bandwidth switching between two frequency regimes. Further, the proposed metasurface has a significant advantage of displaying polarization-insensitive behaviour, unlike the previous fluidically-reconfigured structures. The design has been investigated by illustrating surface current distributions and several parametric variations. Finally, the proposed structure was fabricated using laser etching, and experimentally validated. This work has paved the way towards the realization of reconfigurable metasurfaces with multifunctional characteristics, thus showing great potential in microfluidic technology for diverse applications.
Cultural engagement predicts changes in cognitive function in older adults over a 10 year period: findings from the English Longitudinal Study of Ageing
Authors: Fancourt Daisy , Steptoe Andrew 
Published: June 2018

AbstractThere is increasing evidence that leading an active, socially engaged lifestyle might protect against cognitive decline. The arts have been proposed as potentially beneficial activities due to their combination of cognitive complexity and mental creativity. Yet it remains uncertain which types of arts engagement and what level of engagement is required for potential benefits to accrue. This study therefore explored the association between three types of cultural engagement (visiting museums/galleries/exhibitions, going to the theatre/concert/opera and going to the cinema) and change in cognitive function over 10 years amongst adults aged over 52. Our participants (n = 3,445), drawn from the English Longitudinal Study of Ageing, were assessed in 2004/5 and 2014/15. We measured memory and semantic fluency at baseline and follow-up, analysing results using ordinary least squares regression models. Independent of demographic, health and social confounders, visiting museums/galleries/exhibitions and going to the theatre/concert/opera were associated with a lesser decline in cognitive function. Sensitivity analyses confirmed effects were unaffected by considerations of mobility or dementia diagnoses. However, going to the cinema was found to hold little effect for cognitive preservation. Overall, our results suggest that more frequent cultural engagement is associated with more marked effects, but even annual engagement may be protective.
CAP modifies the structure of a model protein from thermophilic bacteria: mechanisms of CAP-mediated inactivation
Authors: Attri Pankaj , Han Jeongmin , Choi Sooho , Choi Eun Ha , Bogaerts Annemie , Lee Weontae 
Published: June 2018

AbstractCold atmospheric plasma (CAP) has great potential for sterilization in the food industry, by deactivation of thermophilic bacteria, but the underlying mechanisms are largely unknown. Therefore, we investigate here whether CAP is able to denature/modify protein from thermophilic bacteria. We focus on MTH1880 (MTH) from Methanobacterium thermoautotrophicum as model protein, which we treated with dielectric barrier discharge (DBD) plasma operating in air for 10, 15 and 20 mins. We analysed the structural changes of MTH using circular dichroism, fluorescence and NMR spectroscopy, as well as the thermal and chemical denaturation, upon CAP treatment. Additionally, we performed molecular dynamics (MD) simulations to determine the stability, flexibility and solvent accessible surface area (SASA) of both the native and oxidised protein.
Ecological influence of sediment bypass tunnels on macroinvertebrates in dam-fragmented rivers by DNA metabarcoding
Authors: Serrana Joeselle M. , Yaegashi Sakiko , Kondoh Shunsuke , Li Bin , Robinson Christopher T. , Watanabe Kozo 
Published: June 2018

AbstractSediment bypass tunnels (SBTs) are guiding structures used to reduce sediment accumulation in reservoirs during high flows by transporting sediments to downstream reaches during operation. Previous studies monitoring the ecological effects of SBT operations on downstream reaches suggest a positive influence of SBTs on riverbed sediment conditions and macroinvertebrate communities based on traditional morphology-based surveys. Morphology-based macroinvertebrate assessments are costly and time-consuming, and the large number of morphologically cryptic, small-sized and undescribed species usually results in coarse taxonomic identification. Here, we used DNA metabarcoding analysis to assess the influence of SBT operations on macroinvertebrates downstream of SBT outlets by estimating species diversity and pairwise community dissimilarity between upstream and downstream locations in dam-fragmented rivers with operational SBTs in comparison to dam-fragmented (i.e., no SBTs) and free-flowing rivers (i.e., no dam). We found that macroinvertebrate community dissimilarity decreases with increasing operation time and frequency of SBTs. These factors of SBT operation influence changes in riverbed features, e.g. sediment relations, that subsequently effect the recovery of downstream macroinvertebrate communities to their respective upstream communities. Macroinvertebrate abundance using morphologically-identified specimens was positively correlated to read abundance using metabarcoding. This supports and reinforces the use of quantitative estimates for diversity analysis with metabarcoding data.
Hypoxia promotes breast cancer cell invasion through HIF-1α-mediated up-regulation of the invadopodial actin bundling protein CSRP2
Authors: Hoffmann Céline , Mao Xianqing , Brown-Clay Joshua , Moreau Flora , Al Absi Antoun , Wurzer Hannah , Sousa Barbara , Schmitt Fernando , Berchem Guy , Janji Bassam , Thomas Clément 
Published: June 2018

AbstractHypoxia is a common feature of solid tumours that promotes invasion and metastatic dissemination. Invadopodia are actin-rich membrane protrusions that direct extracellular matrix proteolysis and facilitate tumour cell invasion. Here, we show that CSRP2, an invadopodial actin bundling protein, is upregulated by hypoxia in various breast cancer cell lines, as well as in pre-clinical and clinical breast tumour specimens. We functionally characterized two hypoxia responsive elements within the proximal promoter of CSRP2 gene which are targeted by hypoxia-inducible factor-1 (HIF-1) and required for promoter transactivation in response to hypoxia. Remarkably, CSRP2 knockdown significantly inhibits hypoxia-stimulated invadopodium formation, ECM degradation and invasion in MDA-MB-231 cells, while CSRP2 forced expression was sufficient to enhance the invasive capacity of HIF-1α-depleted cells under hypoxia. In MCF-7 cells, CSRP2 upregulation was required for hypoxia-induced formation of invadopodium precursors that were unable to promote ECM degradation. Collectively, our data support that CSRP2 is a novel and direct cytoskeletal target of HIF-1 which facilitates hypoxia-induced breast cancer cell invasion by promoting invadopodia formation.
Radial velocity follow-up of GJ1132 with HARPS
Authors: Bonfils X. , Almenara J.-M. , Cloutier R. , Wünsche A. , Astudillo-Defru N. , Berta-Thompson Z. , Bouchy F. , Charbonneau D. , Delfosse X. , Díaz R. F. , Dittmann J. , Doyon R. , Forveille T. , Irwin J. , Lovis C. , Mayor M. , Menou K. , Murgas F. , Newton E. , Pepe F. , Santos N. C. , Udry S. 
Published: June 2018

The source GJ1132 is a nearby red dwarf known to host a transiting Earth-size planet. After its initial detection, we pursued an intense follow-up with the HARPS velocimeter. We now confirm the detection of GJ1132b with radial velocities alone. We refined its orbital parameters, and in particular, its mass (mb = 1.66 ± 0.23 M⊕), density (ρb = 6.3 ± 1.3 g cm−3), and eccentricity (eb < 0.22; 95%). We also detected at least one more planet in the system. GJ1132c is a super-Earth with period Pc = 8.93 ± 0.01 days and minimum mass mc sinic = 2.64 ± 0.44 M⊕. Receiving about 1.9 times more flux than Earth in our solar system, its equilibrium temperature is that of a temperate planet (Teq = 230−300 K for albedos A = 0.75 − 0.00), which places GJ1132c near the inner edge of the so-called habitable zone. Despite an a priori favorable orientation for the system, Spitzer observations reject most transit configurations, leaving a posterior probability <1% that GJ1132c transits. GJ1132(d) is a third signal with period Pd = 177 ± 5 days attributed to either a planet candidate with minimum mass md sin id = 8.4−2.5+1.7 M⊕ or stellar activity. Its Doppler signal is the most powerful in our HARPS time series but appears on a timescale where either the stellar rotation or a magnetic cycle are viable alternatives to the planet hypothesis. On the one hand, the period is different than that measured for the stellar rotation (~125 days), and a Bayesian statistical analysis we performed with a Markov chain Monte Carlo and Gaussian processes demonstrates that the signal is better described by a Keplerian function than by correlated noise. On the other hand, periodograms of spectral indices sensitive to stellar activity show power excess at similar periods to that of this third signal, and radial velocity shifts induced by stellar activity can also match a Keplerian function. We, therefore, prefer to leave the status of GJ1132(d) undecided.