bluuebunny/crossref_metadata_2025_split huggingface dataset:
Determination of formaldehyde in single cell by capillary electrophoresis with LIF detection
Authors: Fu Yu‐Jia , Chen Liu , Guo Xiao‐Feng , Wang Hong 
Published: January 2019

AbstractAs a small molecule gas, formaldehyde (FA) is the simplest carbonyl active material and plays an important role in the carbon cycle of metabolism. However, due to the volatile nature of the gas, it is difficult to accurately quantify its content, which limits the study of the mechanism of action in life activities. Thus, an efficient approach to quantitative detection of FA in cells especially in single cell is urgent needed. Nevertheless, no method for quantifying FA in single cell has been reported to date. In this work, a fluorescent probe N‐propyl‐4‐hydrazino‐naphthalimide (NPHNA), which has highly desirable attributes and has been applied to living biological samples, was chosen as labeling reagent to detect endogenous FA at single cell level. After optimization of separation conditions, fast baseline separation of the FA derivative N‐propyl‐4‐hydrazone‐naphthalimide product (NPHNA‐FA) and NPHNA was achieved in about 5 min by CE with LIF detection. The detection limit for FA was 5 amol (S/N ratio of 3). The developed method was validated by the measurements of intracellular levels of FA in single cell.
Experimental and numerical analysis of fire scenarios involving two mechanically ventilated compartments connected together with a horizontal vent
Authors: Pretrel Hugues , Vaux Samuel 
Published: January 2019

SummaryThis work deals with an experimental and numerical investigation of a fire scenario involving two rooms mechanically ventilated and connected together with a horizontal vent. The objective is to improve the understanding of the physical phenomena and to assess the capability of computational fluid dynamics (CFD) numerical simulations to predict flow field for such a fire scenario. The study is based on a set of large‐scale fire experiments performed in the framework of the OECD PRISME‐2 project in the DIVA multi‐room facility of the Institut de Radioprotection et de Sûreté Nucléaire (IRSN) and of numerical simulations performed with the ISIS CFD code. The fire scenario consists of two rooms, one above the other, mechanically ventilated and connected to each other with a horizontal vent of 1 m2. The fire is a heptane pool fire located in the lower room. The analysis focuses on the coupling between the burning rate, the flow at the vent, and the configuration of the mechanical ventilation. Several regimes of combustion are encountered from well‐ventilated steady fire to under‐ventilated unsteady and oscillatory fire. The results show that the burning rate is controlled by both the mechanical ventilation and the downward flow from the vent. The numerical simulations highlight the specific pattern of the oxygen concentration field induced by the downward flow at the vent.
Impedance Study on Estimating Electrochemical Mechanisms in a Polymer Electrolyte Fuel Cell During Gradual Water Accumulation
Authors: Cruz‐Manzo S. , Cano‐Castillo U. , Greenwood P. 
Published: January 2019

AbstractIn this study, the change of electrochemical parameters during the gradual water accumulation of a polymer electrolyte fuel cell (PEFC) are estimated using electrochemical impedance spectroscopy (EIS) measurements and an impedance model based on electrode theory during a two‐step oxygen reduction reaction (ORR). EIS measurements were carried out in a 5 cm2 H2/O2 PEFC operated under a dead‐ended configuration of the gas reactants and during gradual accumulation of water. Kramers‐Kronig evaluation demonstrated that the EIS measurements comply with stability and linearity properties and the inductive loops featured at low frequencies are attributed to electrochemical mechanisms within the PEFC and not to instability during gradual water accumulation. The impedance model has been reported in a previous study and simulates inductive loops at low frequencies which are attributed to platinum oxide formation during the ORR. The estimated parameters obtained from this EIS‐modeling analysis can provide an insight into the decrease in PEFC performance during flooding conditions. A further qualitative analysis considering the effect of long‐term water accumulation on the oxygen‐reduction charge transfer resistance is discussed. It is possible to have an insight into the physical mechanisms of the PEFC, by combining different experimental techniques and fundamental theory in a complimentary manner.
Properties and the predictive power of implied volatility in the New Zealand dairy market
Authors: Fernandez‐Perez Adrian , Frijns Bart , Gafiatullina Ilnara , Tourani‐Rad Alireza 
Published: January 2019

AbstractThis study develops a dairy implied volatility index (DVIX), derived from New Zealand Exchange traded options on whole milk powder (WMP) futures. We document an inverse return–volatility relation which is asymmetric, where increases in WMP futures prices are associated with larger absolute changes in the DVIX than decreases. In sample, the results strongly suggest that the DVIX has a high information content regarding conditional variance and that the inclusion of historical information further improves the predictive power. Out of sample, we find that the DVIX provides substantial information about future realized volatility. We also document that a combination of historical volatility and the DVIX provides the best out‐of‐sample forecasts.
Impact of varying anatomic sites on advanced stage and survival of oral cancer: 9‐year prospective cohort of 27 717 cases
Authors: Su William Wang‐Yu , Su Chiu‐Wen , Chang Dun‐Cheng , Chuang Shu‐Lin , Chen Sam Li‐Sheng , Hsu Chen‐Yang , Yen Amy Ming‐Fang , Chiu Sherry Yueh‐Hsia , Fann Jean Ching‐Yuan , Lee Yi‐Huah , Jeng Ya‐Chung , Lee Yi‐Chia , Chiu Han‐Mo , Chen Tony Hsiu‐Hsi , Wang Cheng‐Ping , Chen Mu‐Kuan 
Published: January 2019

AbstractBackgroundTo elucidate the impact of varying anatomic sites on advanced stage of and death from oral cancer.MethodsA total of 27 717 oral cancers mainly from a population‐based visual inspection program in Taiwan from 2004 to 2009 was followed until the end of 2012.ResultsUsing lip cancer as reference, the odds ratios (95% confidence interval [CI]) of advanced stage of cancer were 2.20 (1.92‐2.51) for tongue, 2.60 (2.28‐2.97) for buccal, 2.68 (2.20‐3.28) for floor of mouth, 2.96 (2.52‐3.47) for hard palate, 6.04 (5.17‐7.05) for gingiva, and 10.83 (9.20‐12.74) for oropharynx. The estimated hazard ratios (95% CI) for oral cancer death increased from 1.48 (1.31‐1.67) in buccal, 1.61 (1.43‐1.82) in tongue, 1.68 (1.41‐1.99) in floor of mouth, 1.79 (1.57‐2.05) in gingiva, 1.97 (1.71‐2.26) in hard palate, and 2.15 (1.89‐2.45) in oropharynx.ConclusionDifferent anatomic sites had variations in advanced stage of and death from oral cancer and need vigilant surveillance.
African head and neck fellowships: A model for a sustainable impact on head and neck cancer care in developing countries
Authors: Fagan Johannes J. , Otiti Jeffrey , Aswani Joyce , Konney Anna , Diom Evelyne S. , Baidoo Kenneth , Onakoya Paul A. , Mugabo Rajab M. , Noah Patrick , Mashamba Victor , Kundiona Innocent , Garba Mainasara , Biadgelign Melesse G. , Macharia Chege , Bogale Mesele , Koch Wayne M. 
Published: January 2019

AbstractBackgroundThere is an extreme shortage of head and neck surgeons in Africa. Fourteen head and neck surgeons have completed fellowships in Cape Town and Cameroon. This study determines whether such Africa‐based fellowships are a good model for developing countries by making a sustainable impact on head and neck cancer care.MethodsAn observational study was conducted by emailing questionnaires to past fellows.ResultsAll fellows had returned to teaching hospitals in their counties. Seven established new multidisciplinary cancer teams. Head and neck operations had increased by >335%, as had complexity of the surgery. There was effective transfer of surgical skills to trainees. All considered head and neck fellowships to be the best model to grow head and neck care.ConclusionHead and neck fellowships in developing countries are effective models for establishing training programs and for increasing provision of specialized surgical services in a sustainable fashion.
Case of caval lobular capillary hemangioma mimicking tumor thrombus
Authors: Hashimoto Sho , Obinata Daisuke , Yamaguchi Kenya , Sakurai Fuminori , Yoshida Toshiyuki , Yoshizawa Tsuyoshi , Matsui Tsuyoshi , Mochida Junichi , Masuda Shinobu , Takahashi Satoru 
Published: January 2019

IntroductionWe presented a rare case of caval lobular capillary hemangioma.Case presentationA 66‐year‐old female visited our department complaint with shadow defect in vena cava of right renal hilum appeared on computed tomography for periodically checking 3 years after radical hysterectomy with bilateral ovariectomy. Abdominal computed tomography identified a shadow defect of 35 mm in diameter in the inferior vena cava continuing posteriorly to a 35 mm mass of retroperitoneum. During the total removal of this lesion, we identified the lesion was connected to right ovarian vein. The specimen consisted of microcapillaries which formed reticular structure. Immunostaining of specimens identified positive CD31, CD34, and Factor 8 in all cells. Ki67 antibody was positive at 2–3% of all cells. These findings suggested the tumor was intravenous lobular papillary hemangioma.ConclusionThis is the first report of intravenous lobular papillary hemangioma originated from right ovarian vein and extended to inferior vena cava.
Tests of asset pricing with time‐varying factor loads
Authors: Galvao Antonio F. , Montes‐Rojas Gabriel , Olmo Jose 
Published: January 2019

SummaryThis paper proposes an empirical asset pricing test based on the homogeneity of the factor risk premia across risky assets. Factor loadings are considered to be dynamic and estimated from data at higher frequencies. The factor risk premia are obtained as estimates from time series regressions applied to each risky asset. We propose Swamy‐type tests robust to the presence of generated regressors and dependence between the pricing errors to assess the homogeneity of the factor risk premia and the zero intercept hypothesis. An application to US industry portfolios shows overwhelming evidence rejecting the capital asset pricing model, and the three and five factor models developed by Fama and French (Journal of Financial Economics, 1993, 33, 3–56; Journal of Financial Economics, 2015, 116, 1–22). In particular, we reject the null hypotheses of a zero intercept, homogeneous factor risk premia across risky assets, and the joint test involving both hypotheses.
Release of simvastatin from scaffolds of poly(lactic‐co‐glycolic) acid and biphasic ceramic designed for bone tissue regeneration
Authors: Encarnação Isis C. , Sordi Mariane B. , Aragones Águedo , Müller Carmen Maria Olivera , Moreira Anderson C. , Fernandes Celso P. , Ramos Jeferson V. , Cordeiro Mabel M. R. , Fredel Márcio C. , Magini Ricardo S. 
Published: January 2019

AbstractThe aim of this study was to evaluate the release of simvastatin from scaffolds composed of poly(lactic‐co‐glycolic) acid (PLGA) and biphasic ceramic designed for bone engineering and to assess the physico‐chemical and mechanical properties of the scaffolds. Samples with 30% and 70% porosity were obtained with 0, 2, 5, and 8 wt %. of simvastatin through the solvent evaporation technique and leaching of sucrose particles. Scaffold degradation and simvastatin release were evaluated in phosphate‐buffered saline. Scaffolds were analyzed by scanning electron microscopy and microtomography for two‐dimensional and three‐dimensional morphological characterization of the porosity, connectivity, and intrinsic permeability. The mechanical characterization was conducted based on the compressive strength and the chemical characterization by differential scanning calorimetry and energy dispersive X‐ray spectroscopy. Gradual and prolonged simvastatin release from the scaffolds was observed. The release followed the Korsmeyer kinetics model with the predominance of case II transport for 30% porosity scaffolds, and anomalous behavior for the 70% porosity samples. Simvastatin release was also influenced by the slow scaffold degradation due to the strong chemical interaction between simvastatin and PLGA, as observed by differential scanning calorimetry. The scaffolds presented spherical and sucrose crystal‐shaped pores that resulted in a homogenous porosity, with a predominance of open pores, ensuring interconnectivity. Simvastatin incorporation into the scaffolds and increased porosity did not influence the mechanical properties. The scaffolds presented gradual and prolonged simvastatin release, with satisfactory physico‐chemical and mechanical properties. The scaffolds presented gradual and prolonged simvastatin release, with satisfactory physico‐chemical and mechanical properties, a promise for applications in bone regeneration. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 2152–2164, 2019.
LncRNA‐UCA1 modulates progression of colon cancer through regulating the miR‐28‐5p/HOXB3 axis
Authors: Cui Mingfu , Chen Mingyan , Shen Zhaoming , Wang Ruijie , Fang Xuedong , Song Bin 
Published: January 2019

AbstractEmerging evidence has shown that the long noncoding RNA urothelial carcinoma–associated 1 (UCA1) plays a tumor‐promoting role in colorectal cancer, while miR‐28‐5p shows tumor‐inhibitory activity in several tumor types. However, the mechanisms both of these in colon cancer progression are still unknown. In this work, the detailed roles and mechanisms of UCA1 and its target genes in colon cancer were studied. The results showed that UCA1 was upregulated in colon cancer tissues when compared with the adjacent nonhumorous tissues, as well as in the various colon cancer cell lines, but the expression of miR‐28‐5p showed an opposite trend. Furthermore, a high UCA1 level in colon cancer tissues is positively associated with the tumor size and advanced tumor stages. Functional assays revealed that both UCA1 knockdown and miR‐28‐5p overexpression could inhibit colon cancer cell growth and migration. Further mechanistic studies indicated that UCA1 knockdown played tumor suppressive roles in SW480 and HT116 cells through binding with miR‐28‐5p. We also, for the first time, identified HOXB3 as the target gene of miR‐28‐5p and that HOXB3 overexpression could mediate the functions of UCA1 in cell proliferation and migration of colon cancer cells. In conclusion, our data provided evidence for the regulatory network of UCA1/miR‐28‐5p/HOXB3 in colon cancer, suggesting that UCA1, miR‐28‐5p, and HOXB3 are the potential targets for colon cancer therapy.
RhoGDIβ affects HeLa cell spindle orientation following UVC irradiation
Authors: Doi Natsumi , Kunimatsu Yuuki , Fujiura Kouhei , Togari Hiro , Minagi Kenji , Nakaoji Koichi , Hamada Kazuhiko , Temme Achim , Tatsuka Masaaki 
Published: January 2019

AbstractThe molecular signals that regulate mitotic spindle orientation to determine the proper division axis play a critical role in the development and maintenance of tissue homeostasis. However, deregulation of signaling events can result in spindle misorientation, which in turn can trigger developmental defects and cancer progression. Little is known about the cellular signaling pathway involved in the misorientation of proliferating cells that evade apoptosis after DNA damage. In this study, we found that perturbations to spindle orientation were induced in ultraviolet C (UVC)‐irradiated surviving cells. N‐terminal truncated Rho GDP‐dissociation inhibitor β (RhoGDIβ), which is produced by UVC irradiation, distorted the spindle orientation of HeLa cells cultured on Matrigel. The short hairpin RNA‐mediated knockdown of RhoGDIβ significantly attenuated UVC‐induced misorientation. Subsequent expression of wild‐type RhoGDIβ, but not a noncleavable mutant, RhoGDIβ (D19A), again led to a relative increase in spindle misorientation in response to UVC. Our findings revealed that RhoGDIβ impacts spindle orientation in response to DNA damage.
Motives for Creativity: Exploring the What and Why of Everyday Creativity
Authors: Benedek Mathias , Bruckdorfer Raphaela , Jauk Emanuel 
Published: January 2019

AbstractPeople spend a lot of time on creative activities in their leisure time, but we still know little about what these activities are and what drives them. The literature suggests that several specific motives may be relevant for everyday creative behavior, including enjoyment, expression, challenge, coping, prosocial, social, material, recognition, and duty motives. Across two online studies totaling 750 participants, enjoyment was the strongest motive for everyday creativity, consistent with previous research linking creativity to intrinsic motivation and positive affect. Importantly, however, the relevance of motives differed across creative domains: visual arts, literature, and music were more strongly motivated by expression and coping motives, whereas handicrafts and creative cooking were more strongly motivated by prosocial and recognition motives. Intrinsic motives for creative activities were substantially related to high openness to experience, but explained incremental variance in the prediction of self‐reported creativity as well as rated creative achievements. Together, these findings provide new insights into the motivational basis and function of everyday creativity.
Job satisfaction and stress levels among community pharmacists in Malaysia
Authors: Teong Win Wei , Ng Yew Keong , Paraidathathu Thomas , Chong Wei Wen 
Published: January 2019

AbstractBackgroundCommunity pharmacists have an important role in ensuring safe and effective use of medicines. However, poor job satisfaction and high stress levels may negatively affect job performance.AimThis study evaluated job satisfaction and job stress levels, and associated factors, among community pharmacists in Klang Valley, Malaysia.MethodsA cross‐sectional survey was conducted to assess job satisfaction and stress among community pharmacists. A sample of 286 respondents was selected using stratified‐random clustered sampling. Levels of and factors affecting job satisfaction and stress, as reported by community pharmacists, were analysed.ResultsThere was a moderate level of job satisfaction among pharmacists (mean (±SD) score 3.39 ± 0.42; range 1–5). Job characteristics that provided the highest satisfaction were work environment and interpersonal relationships with coworkers. Stress levels among pharmacists were below the scale mid‐point (mean (±SD) score 1.86 ± 0.44; range 1–4). Responsibility for patient care was perceived to contribute most to job stress. Ethnicity (p = 0.026) and average number of prescriptions filled daily (p = 0.016) significantly affected job satisfaction, whereas job stress was significantly affected by age (p < 0.001), years of experience (p = 0.003) and number of working hours/week (p = 0.003).ConclusionsThis study demonstrates the effects of various demographic and job‐related variables on community pharmacists’ job satisfaction and stress. Further research is required to gain a better understanding of factors affecting job satisfaction and stress among pharmacists across Malaysia, and their effects on professional practice and patient care.
Patterns of medication prescription by dementia diagnosis in Australian nursing home residents: a cross‐sectional study
Authors: Liu Enwu , Dyer Suzanne M. , Whitehead Craig , O'Donnell Lisa Kouladjian , Gnanamanickam Emmanuel S. , Harrison Stephanie L. , Milte Rachel , Crotty Maria 
Published: January 2019

AbstractBackgroundCurrent information on the patterns of medication use in nursing home residents living with dementia is conflicting.AimThe aim of this study was to investigate medication use and its associations with dementia diagnosis in Australian nursing home residents.MethodsA cross‐sectional study of 541 residents from 17 Australian nursing homes was performed.ResultsOver 12 months, nursing home residents were prescribed a mean (±SD) of 14.5 ± 6.8 medications each. Approximately 95% of residents were prescribed medications for the nervous system, and 94% were prescribed medications for the alimentary tract and metabolism. After adjustment for potential confounders, those with dementia were less likely to be prescribed medications for the cardiovascular system (odds ratio (OR) 0.45, 95% confidence interval (CI) 0.27–0.77, p = 0.0032) and respiratory system (OR 0.38, 95% CI: 0.25–0.60, p < 0.0001). Further analysis of specific medications showed that residents with dementia were more likely to be prescribed risperidone (OR: 9.38, 95% CI: 4.01–21.94, p < 0.0001), buprenorphine (OR 3.37, 95% CI: 1.78–6.36, p = 0.0002) and trimethoprim (OR 1.78, 95% CI: 1.06–2.97, p = 0.0282), but less likely to be prescribed glyceryl trinitrate (OR 0.42, 95% CI: 0.19–0.94, p = 0.0340) and salbutamol (OR 0.34, 95% CI: 0.2–0.6, p = 0.0002) in addition to other specific medications.ConclusionsIncreased awareness of possible undertreatment of cardiovascular and respiratory conditions, and possible overtreatment of urinary tract infection, in those living with dementia in nursing home settings may improve management of this vulnerable population.
Acute opioid withdrawal following administration of oral oxycodone–naloxone due to portosystemic shunts
Authors: Peterson Adam R. , Aminian Parnaz , Hey Penelope C. , Gow Paul 
Published: January 2019

AbstractBackgroundThe management of pain in patients with advanced liver disease is a clinical challenge. Initial pharmacokinetic safety data advised against the use of oxycodone–naloxone in this population, but in clinical practice it is commonly used. Our case aims to illustrate a potential mechanism by which administration of oxycodone–naloxone can cause systemic opioid antagonism and harm to patients.Clinical detailsA 45‐year‐old man received two separate doses of oxycodone–naloxone in the immediate postoperative setting, resulting in symptoms and signs consistent with acute opioid withdrawal. A review of his imaging revealed significant portosystemic shunts.OutcomesPortosystemic shunts in patients with advanced liver disease may lead to a decrease in the Phase II hepatic metabolism of naloxone and increased systemic levels. In the case of someone with pre‐existing opioid dependence, this may precipitate acute opioid withdrawal.ConclusionThe use of oxycodone–naloxone preparations should be avoided in patients with liver disease and portosystemic shunts.
Investigation of opioid prescribing in the postoperative setting among opioid‐naïve surgical patients: a 6‐month observational study
Authors: Kardell Luke David , Hilmer Sarah Nicole , Crane Jennifer A. , MacPherson Ross , Kim Tae Yeon (Lisa) , Gnjidic Danijela 
Published: January 2019

AbstractThere is sparse information on patterns of opioid use among opioid‐naïve surgical patients. This prospective observational study investigated patterns of opioid prescribing after hospital discharge, opioid use at 1 week and 1, 3 and 6 months after discharge, and associations between opioid use, pain control and opioid‐related difficulties in opioid‐naïve surgical patients (age ≥18 years, not prescribed opioids in the previous 12 months, expected hospital length of stay >24 h after surgery). Of 128 participants recruited, 56.3% (n = 72) were male with a median (interquartile range) age of 66 (50.7–73.0) years; 38.3% (n = 49) were discharged with an opioid prescription postoperatively. After discharge, 37.3% (n = 25/67), 12.3% (n = 8/65), 12.5% (n = 6/48) and 2.4% (n = 1/42) of participants had used an opioid within 1 week and 1, 3 and 6 months, respectively. Low levels of pain were experienced overall postoperatively. There was considerable variation in opioid‐related problems and concerns over time, with the most common being the desire to cease the opioid. Follow‐up response rates were low, which limits the conclusions that can be drawn from the data. However, the findings indicate that opioids are commonly prescribed at discharge to opioid‐naïve surgical patients, with significant decline in use over time.