bluuebunny/crossref_metadata_2025_split huggingface dataset:
PO-150 Different gradient altitude training on the impact of RET、 EPO and RBC
Authors: Zong Lei 
Published: October 2018

Objective This study is based on the regional characteristics of Guizhou Province, athletes living in the subplateau and training in the plateau-plain and the high plateau-plain.The effects of altitude training on hematological indexes of EPO、RBC and RET.To evaluate the two training modes is more beneficial to endurance runners and explore which one is better. To provide scientific reference for sports training practice.
Methods Twenty male endurance runners and divided into two groups (all living on subplateau )： plateau-plain group and high plateau-plain。One group reached to the 1900 m plateau and the other one reached to the 2900 m plateau,training 3 weeks ；The two groups then reached to the plain for 3 weeks of training。Finally arrived at the sub-plateau for performance testing.Using multivariate analysis of variance to test significant differences between groups，The data are represented by the standard deviation of the average（）.P＜0.05 is a significant difference，P＜0.01 is a very significant difference.
Results 1 EPO
There is no significant difference in EPO between 1900m plateau and 2900m plateau athletes.at the end of plain the amplitude of elevation and basic value of high altitude group is more than that of high altitude group.
2 RBC
Comparison of RBC between plateau group and high altitude group during the period of plateau, there is significant difference at the end of plateau (P=0. 049 < 0. 05), and there is a significant difference between the two groups at the end of plateau training (P=0. 043 < 0. 05). The change trend of the two groups is the same, but the increasing is more than the high plateau group, and the decreasing is relatively less during the plain period, and it showed an upward trend at the end of the plain period.
3 Ret
There is significant difference in RET between the two groups (P=0. 018 < 0. 05) when the endurance runners arrived at the plateau at the first weak. RET at 2900m altitude is significantly higher than that at 1900m altitude, and at the end of the plateau, the increase of RET in the high plateau group is still higher than that in the plateau group. There is no significant difference between the two groups during the plain period.
Conclusions The influence of altitude training on the EPO of the SUBPlateau athletes is more profound than that of the plateau group. During the first week of plateau, EPO decreased significantly, but it fluctuated up and down later, and the two groups are on upward trend during the period of plain. However, the percentage of elevation in the plateau is higher in the high altitude group than in the high altitude group.
After plateau training, chose 2900m plateau for training, and the hematopoietic function of bone marrow is improve greatly, nutrition supplement should be paid attention to after plateau training. To prevent the effect of RBC reduction.
PO-157 Effect of exercise training on mitochondrial content after ischemia in the cerebral cortex of rat
Authors: Shu Tian , Bo Hai , Zhang Yong 
Published: October 2018

Objective To investigate the effect exercise training on mitochondrial content after ischemia in the cerebral cortex, and the balance between mitochondrial biogenesis and mitophagy in this process.
Methods Male Sprague-Dawley rats were randomly divided into 4 groups (n = 8): Sham operation group (SH), Sham operation and training group (ST), ischemia control group (IC), ischemia and training group (IT). The ischemia model rats were subjected to right middle cerebral artery occlusion which was produced by the intraluminal suture technique. The rats in the sham group underwent the same procedure except for the occlusion of the middle cerebral artery. Fourteen days after operation, the exercise training animals were exercised on a motor-driven rodent treadmill at a speed of 10 m/min, 5% grade for 30 min/day, 5 days/week for 4 weeks. Mitochondrial membrane potent was determined using JC-1. ATP synthesis capacity was determined using a bioluminescence technique. The protein expression of VDAC-1, COXIV, PGC-1α, Tfam, PINK1 and Parkin in cerebral cortex were detected by Western-blotting.
Results Compared with SH group, ischemia attenuated mitochondrial membrane potential, ATP synthesis capacity, and the expression of VDAC-1, COXIV, PGC-1α, Tfam in cerebral cortex (P<0.05~0.01). Furthermore, ischemia increased the expression of PINK1 and Parkin (P<0.05~0.01). Compared with IC group, exercise training elevated mitochondrial membrane potential, ATP synthesis capacity, and the expression of VDAC-1, COXIV, PGC-1α, Tfam, PINK1, Parkin in IT group (P<0.05~0.01).
Conclusions A combination of reduced mitochondrial biogenesis and increased mitophage seems to be responsible for the decrease in mitochondrial content after ischemia. Exercise training after ischemia elevated mitochondrial content and function in cerebral cortex, which may be mediated by appropriately increase and co-regulation of mitochondrial biogenesis and mitophage.
PO-158 Association Of Glucose Metabolism and Physical Activity By Chronotype in Elderly Japanese Adults
Authors: Zhang Qiwei , Murata Hiroko , Ito Tomoko , Kawakami Ryoko , Shibata Shigenobu , Higuchi Mitsuru 
Published: October 2018

Objective Chronotype is a trait determining individual circadian preference in behavioral and biological rhythm relative to external light-dark cycle. Although evening chronotype has been reported to be associated with bad glucose control and low physical activity in middle-aged adults, it is not known whether it is true in elderly people. Therefore, the aim of this study was to investigate the relationship between glucose metabolism and physical activity by chronotype (circadian rhythm) in elderly Japanese adults.
Methods A cross-sectional study was conducted in 178 adults (72 men and 106 women), aged 60-79 years, who were classified into three chronotype groups, ”definitely morning type (DMT)”, “moderately morning type (MMT)” and “neither type (NET)”, based on the Morningness/Eveningness Questionnaire scores (MEQ-Score). All participants were required to report their daily rhythms of behavior, such as meal time and sleep-wake cycle. Additionally, their physical activity were measured by an uniaxial accelerometer (Kenz Lifecorder EX, SUZUKEN, Nagoya, Japan). Energy intake was assessed by a brief self-administered diet-history questionnaire. Blood was drawn for biochemical analysis after an overnight fast.
Results BMI and serum insulin in the DMT group was significantly higher than MMT and NET groups in male. The DMT group had a significantly shorter time interval between dinner and sleep than the other two groups, both in male and female. After adjustment for covariates (age, smoking and alcohol status, energy intakes, moderate-vigorous physical activity (MVPA) and sleep duration), the BMI, serum insulin, fasting blood glucose (FBG) and HOMA-IR in the DMT group was significantly higher than other groups. However, after adjustment for the time interval between dinner and sleep, the significant difference had disappeared.
There was significant difference in terms of low-intensity physical activity between male and female. And MVPA in the DMT group, the subjects with low MVPA (<23Mets·hour/week) had higher FBG, serum insulin and HOMA-IR than the subjects with high MVPA (>23Mets·hour/week) in men, but not in women.
Conclusions This study demonstrated that the DMT group with early sleep-wake lifestyle had higher BMI, FBG, serum insulin and HOMA-IR, specially in elderly Japanese men with low MVPA, but not in women.
PO-170 Epidemiology of children's febrile seizures and prospects for health education
Authors: Hu Liang Yi , Zhen Zhi Ping 
Published: October 2018

Objective In this paper, through the epidemiological characteristics of febrile seizures (FS) and the status quo of health education, the non-drug prevention system based on scientific physical activity intervention is gradually formed in the future, so as to establish "knowledge, pr34evention, medical, nursing ,sports nutrition” The protection and maintenance of the five-in-one sports promotion health network lays the theoretical foundation.
Methods Through domestic and foreign literatures, CNKI, Pubmed, Web of science and other databases are reviewed.
Results (1) The exclusion criteria of FS are similar at home and abroad, only the definitions of onset age are different, the disease has age dependence.(2) The index of FS prognosis health assessment is single. There are few reports on the post-exercise ability and physical changes of FS children. And there is a lack of preventive health care related to exercise and brain health.(3) The descriptive study of FS in China lacks the incidence rate based on large-scale population samples, and the interventional research is limited to the clinic. The prevention and prognosis interventions for this disease lack relevant health education guidelines and intervention programs for kindergartens, schools and communities.(4) In the process of continuous seizures, hypoxia can cause transient or long-term organ damage in the body, affecting the development of the nervous system. The effects of FS on physical fitness are mainly concentrated on balance ability, systemic coordination ability and motor discrimination ability. Balance, sensitivity and coordination quality are related to nervous system, skeletal muscle and sensory organs.
Conclusions The research on the epidemiological characteristics of FS in China needs to be further improved. Prognosis and health assessment should be complementary to physical health and medical health. For children with history of febrile seizures, exercise as a simple, convenient, non-burden, drug-free effective way to promote central nervous system and brain health, has a positive effect on improving the immune level of children with FS and improving the prognosis of brain damage. Provide new ideas for prognosis and prevention, and promote health education and health communication of febrile seizures.
PO-175 Interruption of mitochondrial homeostasis inhibits Irisin biosynthesis in C2C12 Myoblast
Authors: Zhang Ziyi , Wang Tiantian , Bo Hai , Zhang Yong 
Published: October 2018

Objective Irisin is a PGC-1α dependent myokines suggested to provide the basis for the crosstalk between skeletal muscle and other organs. PGC-1α induces the expression of a membrane protein fibronectin type III domain-containing protein 5 (FNDC5), and exercise triggers the cleavage of FNDC5 to secrete irisin into the bloodstream. It was found that low level of Irisin in circulation was accompanied with impaired skeletal muscle mitochondrial homeostasis during aging. However, the relationship between mitochondrial homeostasis and Irisin induction is merely correlative. This study established mitochondrial homeostasis interrupt model in order to explore whether mitochondrial homeostasis involved in regulation of Irisin synthesis.
Methods C2C12 myoblasts were treated with OPA1 siRNA for 48 hours. Mitochondrial energy metabolism was measured with seahorse XF24 Extracelluar Flux Analyzer. Mitochondrial reticulum morphology was monitored with confocal laser scanning microscopy. Protein expressiont of Mfn1, Mfn2, OPA1, Drp1, FNDC5, PGC-1α, SirT1and GCN5 were measured with Western blot. Content of Irisin in culture medium was determined with Elisa.
Results Compared with control, in OPA1 siRNA myoblast, the expression of Mfn2(-35%, P<0.01), Drp1(-45%, P<0.01), Irisin(-10%, P<0.05), FNDC5(-33%, P<0.01), PGC-1α(-48%, P<0.01), SirT1(-40%, P<0.01), GCN5(-22%, P<0.05) proteins and the levels of cell membrane potential(-92%, P<0.01) and the basic OCR(-20%, P<0.01), ATP potential(-20%, P<0.05), max respiration capacity of mitochondrial(-29%, P<0.05) were decreased significantly. The expression of Mfn1(+39%, P<0.01) was increased significantly.
Conclusions Interruption of mitochondrial homeostasis inhibits Irisin biosynthesis. This may be related to mitochondrial ATP loss and imbalance energy-sensitive signaling SirT1/GCN5, which in turn suppress PGC-1α and its downstream Irisin.
Supported by NSFC (No. 31771320, 81370454, 31110103919).
PO-179 Prevention of sarcopenia and regulation of the ERK1/2 and p38MAPK phosphorylation by resistance training
Authors: Chen Hao , Weng Xiquan , Lin Wentao , Zhai Sanhua 
Published: October 2018

Objective Study of the effect of treadmill resistance training on the cross section and phosphorylation of ERK1/2 and p38MAPK of gastrocnemius of aging rats, and explore the role of resistance training in prevention and regulation of sarcopenia.
Methods 11-month-old male Sprague-Dawley (SD) rats (n = 40, SPF grade, initial body weight 776.9 ± 50) were raised in various cages in a room with a room temperature of 23 ± 2°C, a humidity of 40–60%, and a natural light/dark cycle. After eight months, the treated rats were then divided into one sedentary group (control, n = 8/group) and four groups for resistance training (GRT 1-GRT 4, GRT for Group for Resistance Training, n = 8/group) lasting for 8 weeks. For resistance training, GRT1 to GRT4 carried no loading, 30% maximum loading, 50% maximum loading and 70% maximum loading, respectively. The protocol for treadmill running was as follows: 15m/min (speed), 35°C (slope), 15s each time, an interval for 30s between two runnings, four runnings as one training, an interval for 3mins between two training, three training as one cycle, two cycles one training day, and one-day break between two training days. The morphology and cross section of gastrocnemius and the phosphorylation of the ERK1/2、p38MAPK proteins in the rats were examined after 8 weeks’ training.
Results Compared with the control, GRT1-GRT4 displayed relatively better aligned muscles fibers and noticeably larger cross-sectional area (P＜0.05). The content of p-ERK1/2 (phosphorylated ERK1/2) displayed a descending trend as the loading increased compared with the control (GRT1 VS control, P＞0.05; GRT2 VS control, P＜0.05; GRT3 VS control, P＞0.05; GRT4 VS control, P＞0.05). However, the pairwise comparison revealed that only the difference between GRT1 and GRT2 was significant. Similarly, the content of p-p38MAPK (phosphorylated p38MAPK) displayed a descending trend as the loading increased compared with the control (GRT1 VS control, P＞0.05; GRT2 VS control, P＞0.05; GRT3 VS control, P＜0.01; GRT4 VS control, P＜0.01). Besides, the pairwise comparison revealed that the difference between GRT1 and GRT3 was significant (P＜0.05) and that between GRT1 and GRT4 was even highly significant (P＜0.01).
Conclusions Resistance training with middle-class intensity or above can prevent sarcopenia. ERK1/2 and p38MAPK were subject to high-level phosphorylation in aging skeletal muscles, which resulted from the stress-like status of the aging body. Treadmill training of with middle-class intensity or above can reduce ERK1/2 and p38MAPK phosphorylation in aging skeletal muscles of rats, which indicates that resistance training can effectively prevent sarcopenia by inhibiting ERK1/2 and p38MAPK phosphorylation.
PO-180 Effect of Accumulated Exercise and Continuous Exercise on Energy Metabolism
Authors: Wang Songtao , Li Dan , Shuai Xiangyu 
Published: October 2018

Objective Sedentary behavior is the main feature of modern lifestyle, which relate to most chronic diseases. Therefore, it has great significance in both theory and practice of weight control and health promotion to find some exercise modes which can effectively break sedentary lifestyle and increase daily physical activity. The multiple short-time exercise scattered throughout the whole day was called Accumulated Exercise. Accumulated exercise can break the sedentary lifestyle, but our understanding about the energy metabolism and its influence on appetite is limited. This study measured the energy expenditure of two kind of exercise with equal workload (moderate intensity continuous exercise and accumulated exercise) as well as their impact on appetite, in order to enhance the effect and adherence of exercise intervention in weight control and health promotion.
Methods 16 healthy college students (8 male and 8 female) were recruited. Three experiments were randomly carried out with one week’s interval, which were recorded as rest control, accumulated exercise and continuous exercise. Accumulated exercise and continuous exercise were performed on treadmill with 65%VO2max intensity. Continuous exercise was lasted 1 hour without interval, while accumulated exercise was divided 4 times, 15min each, with 1 hour’s interval. The total exercise load (running distance) of the two exercise is equal.
The volume of consumed O2 and generated CO2 in pre-exercise, during exercise, and post-exercise (0-4h) were dynamically monitored. The gastric electricity and appetite scale after exercise, as well as the basal metabolic rate of the next morning was also measured. Using the Peronnet and Massioctte equations to calculate the carbohydrate oxidation, fat oxidation, and the total energy expenditure during exercise and post-exercise (0-4h), then compare the differences between the two kind of exercise in energy expenditure, appetite, and gastric electrical activity.
Results Compared with continuous exercise with equal workload, accumulated exercise can increase fat oxidation (and %) during exercise and exercise interval, as well as the total energy expenditure in exercise interval, especially in male students. However, accumulated exercise has the potential to promote stomach emptying, and then increase the appetite (before bedtime). While the continuous exercise can significantly suppress the appetite of the next meal.
Conclusions Accumulated exercise and continuous exercise has different advantage in promoting energy (fat) expenditure and suppressing appetite respectively, so they should be jointly applied to enhanced effects of exercise intervention on weight control.
PO-188 Effects of Tai-Chi exercise on blood lipids, inflammatory factors and baPWV of middle-aged and elderly people
Authors: Li Ningchuan , Lian Yin Xia , Wen Shen Shi , Jun Xia , Yan Li Shu , Long Hu Yu 
Published: October 2018

Objective To investigate the interventional effects of 12 months Tai-Chi exercise on blood lipids, inflammatory factors and the interventional mechanism of exercise in arteriosclerosis of middle-aged and elderly people
Methods 30 in middle-aged and elderly people with average age of 63.50±2.91 joined 12 months taijiquan exercise that conducted 60 minutes ecth time, six times per week. Left brachial-ankle pulse wave velocity (L-baPWV), right brachial-ankle pulse wave velocity(R-baPWV) , left ankle brachial index (L-ABI), right ankle brachial index(R-ABI), serum triglyceride(TG), total cholesterol(TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), interleukin 6 (IL-6) and hypersensitive c-reactive protein (hs-CRP) were detected at 3 time points including before exercise programme, by the end of exercise for 6th and 12th month.
Results ①Compared with pre-exercise, the R-baPWV and R-ABI of the elderly people were decreased at the end of the 6th month, and the L-baPWV, R-baPWV, R-ABI and L-ABI were decreased significantly at the end of the 12th month. ②Compared with pre-exercise, TC and LDL-c were declined markedly (P<0.01) at the end of the 6th and the 12th month, and there was no difference of the level of TG and LDL-c between pre-exercise and post-exercise.③Compared with before exercise, the subjects had significantly decreased IL-6 at the end of 6th months of exercise (P < 0.01), and the decrease of hs-CRP was not obvious. At the end of 12 th months, IL-6 and hs-CRP decreased significantly (P < 0.01). ④According to the correlation analysis, baPWV were positively correlated with TC, IL-6 and hs-CRP.
Conclusions 12 months of exercise intervention can effectively reduce the elderly baPWV and ABI level, improve the blood pressure, blood lipid and body inflammation levels, thus to prevent the happening of the atherosclerosis development plays an important role.
PO-194 Urinary metabolomics study on effects of Rhodiola on Marathon Amateurs after Quantitative Exercise Load
Authors: Han Yumei , Jia Yi , Jia Yi , Tian2 Junsheng , Zhang Bo , Xue Xiong 
Published: October 2018

Objective To study the effect of Chinese medicine Rhodiola on oxidative stress injury in amateur marathon runners after quantitative exercise load (20 km) and explore its mechanism.
Methods Eight marathon amateurs were divided into four groups according to different test time, including before and after quantitative exercise load (group C and group CE), before and after quantitative exercise load after taking a month of the Rhodiola (group MC and group ME). The participants had serum superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), total antioxidant capacity (T-AOC) and malondialdehyde (MDA), as well as myocardial enzyme index - creatine kinase (CK), lactate dehydrogenase (LDH), creatine kinase isoenzyme (CK-MB) and glutamic oxaline aminotransferase (AST/GOT) activity evaluated. In order to further explore the mechanism of action of Rhodiola, the urine was analyzed by 1H-HMR metabonomics technique.
Results 1) Compared with group C, the activity of serum CK, LDH and AST/GOT of group CE increased significantly (P<0.01 or P<0.05), MDA content and SOD activity also increased significantly (P<0.05). A total of fifteen potential biomarkers were found in group CE, such as valine, lactic acid, 2- hydroxy isobutyric acid and so on (P<0.05 and VIP>1). 2) Compared with group CE, the activity of serum CK and AST/GOT and the content of MDA of group ME decreased significantly (P<0.05), eleven metabolites among the fifteen potential biomarkers reverted significantly (P<0.01 or P<0.05), which mainly involved in 4 metabolic pathways including alanine, aspartic acid and glutamic acid metabolism and so on.
Conclusions Rhodiola can enhance the antioxidant capacity and improve myocardial damage of marathon amateurs after quantitative exercise load, which may be due to increased synthesis and utilization of aminoacyl-tRNA and other amino acids.
PO-199 Meta analysis of Exercise Intervention on Dyslipidemia in recent 6 years
Authors: Ye Chengjie 
Published: October 2018

Objective To investigate and evaluate the evidence of exercise intervention in dyslipidemia, this study aims to make a systematic evaluation of exercise intervention in recent 6 years and a summary evaluation of meta analysis.
Methods The electronic database was searched including CNKI, Wanfang. The Chinese key words, dyslipidemia, exercise, physical activity, acute exercise, aerobic exercise, exercise training, etc., were collected from various databases from January 2013 to July 2018. After extracting the data,RevMan5.3 software was used to analyze the merging effect.
Results 2066 subjects were included in 17 articles, 1026 in the experimental group and 1040 in the control group. The results showed that: (1) Total cholesterol (TC) SMD=-0.74 (95% Cl: -1.07- 0.41,P < 0.00001); (2) Triglyceride (TG) SMD=-0.98 (95%Cl:-1.39 -0.56,P < 0.00001); (3) Low density lipoprotein cholesterol (LDL-C )SMD=-1.06 (95%Cl: -1.63-0.50, P < 0.00001); (4) High density lipoprotein cholesterol (HDL-C)SMD = 0. 66 (95% Cl: 0. 39~0.93, P < 0.00001).
Conclusions Exercise intervention can effectively reduce the plasma TCG LDL-C concentration and increase the plasma HDL-C concentration.