bluuebunny/crossref_metadata_2025_split huggingface dataset:
The expression of CCL11/eotaxin, CCR3 receptor and eosinophil peroxidase in tumor tissue in gastric and colon cancers
Authors: Kolobovnikova Yu. V. , Yankovich K. I. , Romanova E. V. , Dmitrieva A. I. , Novitskiy V. V. , Urazova O. I. 
Published: October 2018

The purpose of the study was to analyze the expression of CCL11/eotaxin, CCR3 receptor to eotaxin and eosinophil peroxidase (EXP) in tumor tissue and its relation to tissue eosinophilia in gastric and colon cancers.Materials and methods. 52 patients with gastric cancer and 55 patients with colon cancer were examined. The material of the study was samples of malignant tumors of the stomach and large intestine obtained during a surgery. The expression of CCL11/eotaxin, CCR3 and EXP in the tumor tissue was evaluated by immunohistochemical method. For statistical processing of the results, one-factor dispersion and correlationregression (by Spearman) methods of analysis were used.Results. High expression of CCL11/eotaxin by tumor cells is typical of stomach and colon cancers with eosinophilic infiltration of the tumor tissue. The CCR3 receptor to eotaxin on the cell membrane of infiltration of the tumor tissue in gastric and colon cancers is found in 100% of cases. Expression of CCR3 (at stomach cancer) and EXP (at stomach and colon cancers) by cells of the tumor microenvironment with eosinophilic granulocytes infiltration is significantly higher than in the cells of the tumor microenvironment without eosinophilic infiltration.Conclusion. Tissue eosinophilia in gastric and colon cancers develops due to the ability of transformed malignant cells to produce CCL11/eotaxin that mediates the attraction of CCR3-expressing eosinophil granulocytes in the tumor tissue. High level of EPX (marker enzyme of eosinophils) expression by cells of tumor microenvironment in the gastric and colon cancers with tissue eosinophilia suggests the expressed cytotoxic potential of eosinophilic granulocytes, which can be directed against tumor cells.
Ubiquitin and regulation of apoptosis in Jurkat cells
Authors: Nosareva O. L. , Stepovaya E. A. , Ryazantseva N. V. , Shakhristova E. V. , Orlov D. S. , Novitsky V. V. 
Published: October 2018

Introduction.One of the crucial tasks in medicine is studying the molecular mechanisms of selective management of tumor cell apoptosis following conformational changes in protein molecules (ubiquitination).The purpose of the study. The aim of the project is to establish the role of ubiquitin and ubiquitinligase in dexamethasone-induced apoptosis in Jurkat cells.Materials and methods.The study was carried out on the Jurkat tumor cell line (intact cells and cells cultured in the presence of an apoptosis inducer dexamethasone in the final concentration of 10 µmol. In intact and dexamethasone-affected Jurkat cells, implementation of apoptosis and the amount of FAS-, TNF Receptor 1 and cells with reduced mitochondrial membrane potential were assessed by flow cytometry using FITC-conjugated Annexin V and Propidium Iodide. The levels of NF-κB, Apaf-1, ubiquitin and ubiquitin ligase were determined by Western blot analysis. The activity of caspase-3 was measured by spectrofluorometry.Results.When adding the apoptosis inducer dexamethasone to the Jurkat cell culture, we registered a fall in the concentration of ubiquitin and a rise in the level of ubiquitinligase against the backdrop of activated receptor(an increase in the amount of Annexin V positive cells, FASand TNF Receptor 1) and mitochondrialmediated (an increase in the number of cells with reduced mitochondrial membrane potential and elevation of Apaf-1 level) pathways of apoptosis, as opposed to the intact cell culture. We estimated the completion of apoptosis by determining the activity of caspase-3 in the investigated tumor cells.Conclusion.The obtained findings allow the conclusion that ubiquitination of regulatory and effector proteins in programmed cell death is one of the molecular mechanisms that regulates and selectively controls apoptosis in Jurkat cells.
Brain Drain Phenomenon in Romania: What Comes in Line after Corruption?
Authors: Iacob (Bâra) Raluca 
Published: October 2018

Romanians’ rapidly increasing exodus over the borders, in the last years, propelled Romania to the top countries with the highest international migration rates worldwide. A rate of 7.3% per annum, recorded between 2000 and 2015, placed Romania second, after Syria (United Nations, 2016). Between 1990 – 2017, Romania registered the highest rise in the migration stock among all EU states – 287 per cent (The World Bank, 2018a). The boost of the migration phenomenon was supported by significant changes, including in the migrants’ profiles, in terms of their level of education, consequently the loss of human capital represented by the highly skilled Romanians already has an impact on the key sectors of the Romanian economy. The highly skilled manpower shortage has been a constant topic on the public agenda, especially after Romania’s integration in the European Union (2007), and after the burst of the most recent financial crisis (2008). The number of highly educated employees (tertiary education and upper secondary and post-secondary attainment) decreased in Romania, negatively affecting the key sectors of the economy. Furthermore, research shows that Romania has the lowest percentage of tertiary education graduates from the EU, with 26.3% for the age segment of 30 – 34 years (Eurostat, 2018a).
The paper aims to show recent data on the determinant causes of the brain drain phenomenon in Romania (push factors) and to generate a classification of the three categories of mainly possible determinants: economic, socio-political and organizational factors, based on the results of an online survey addressed to Romanian high-skilled migrants.
Peculiarities of Primary MARTX Cytotoxin Structure in Non-Toxigenic <i>Vibrio cholerae</i> strains of Different serogroups
Authors: Monakhova E. V. , Arkhangel’skaya I. V. , Pisanov R. V. , Titova S. V. 
Published: October 2018

Objective of the investigation was a comparative bioinformatics analysis of rtxA gene translation products of Vibrio cholerae strains isolated from patients and environmental objects. Materials and methods. 32 Vibrio cholerae strains from the Rostov-on-Don Research Anti-Plague Institute collection were used. DNA sequencing was conducted on the MiSeq platform (Illumina), gene identification and analysis was carried out by means of BioEdit 7.2.5, BLASTN 2.2.29, Blastp, Vector NTI Advance 11 software programs. Results and conclusions. The rtxA genes of the studied strains were represented by multiple alleles. AlignX-analysis of their deduced products divided 32 proteins into 3 separate clusters. The first one included proteins of O1 and nonO1/nonO139 strains similar to the prototype, the second – nonO1/nonO139 only, the third – O139. Blastp-analysis revealed that the proteins of the first cluster retained all domains characteristic of MARTX – ACD (actin cross-linking), RID (Rho GTFase inactivation), CPD (cysteine protease) and ABH (alpha-beta hydrolase) which evidences the probability of manifestation of the typical activity. In 3 strains a new Hia domain (of putative adaptation factor) was detected. The proteins of the second cluster lacked ACD but formed an additional RID; two of which lacked ABH too, but in one, VIP2 domain (of actin modification) appeared and in another – Hia. These data are in accordance with the presumption of J. Dolores, K.J.F. Satchell (2013) on the possible converting of actinomodulator MARTX into toxins with other mechanisms of action. The proteins of O139 vibrios shared all specific domains except from ACD. For a number of altered proteins revealed by us full homologues were found in NCBI gene bank, belonging to isolates from other regions of the world. The presence of similar determinants in the genomes of strains of different origin points to non-occasional character of their conservation. It is possible that certain strains “deliberately” alter those genes or switch off the synthesis of the high-molecular toxin completely with a view to energy-saving.
Assessment of epidemiological and epizootiological situation on tularemia in the Republic of tatarstan
Authors: Savitskaya T. A. , Popov V. P. , Trifonov V. A. , Milova I. V. , Isaeva G. Sh. , Reshetnikova I. D. 
Published: October 2018

Objectiveof the study was to assess epidemiological and epizootiological activity of natural tularemia foci in the Republic of Tatarstan for further forecasting of the developments and working out the tactics for implementation of preventive and anti-epidemic measures.Materials and methods. We utilized statistical data, reports of the Center of Hygiene and Epidemiology in the Republic of Tatarstan, and literature sources for the analysis.Results and conclusions. Since 1995, the incidence among the population in Tatarstan has not been registered. Natural tularemia foci are situated in 19 enzootic as regards the infection administrative districts in the territory of the Republic. Epizootiological monitoring of the natural foci suggests that over the last four years their activation occurred. In this contest realization of complex anti-epidemic measures, regulated by the current normative-methodological documents remains relevant, as well as timely and effective diagnostics, including among the patients with a similar to tularemia symptoms.
Pengaruh Model Learning Cycle Terhadap Keterampilan Proses Sains Siswa Pada Mata Pelajaran IPA Kelas III SD Negeri Harja Mekar 03 Kec. Cikarang Utara
Authors: Anisa Ririn Nurcholidah 
Published: October 2018

Penelitian ini dimaksudkan untuk mengetahui pengaruh penggunaan model learning cycle terhadap keterampilan proses sains siswa kelas III SD Negeri Harja Mekar 03 Kec. Cikarang. Penelitian ini menggunakan pendekatan kuantitatif, dengan metode penelitian quasi experiment dan desain penelitian nonequivalent control group design. Teknik pengambilan sampel menggunakan teknik sampling purposive. Populasi yang digunakan adalah 44 siswa dengan jumlah sampel kelas eksperimen 23 siswa dan kelas kontrol 21 siswa. Teknik analisis yang digunakan adalah uji-t dengan taraf signifikan 0,05.
Hasil penelitian yang diperoleh menunjukkan bahwa nilai rata-rata posttest kelas eksperimen sebesar 82,17 lebih tinggi dibandingkan nilai rata-rata posttest kelas kontrol sebesar 79,38. Hasil dari pengujian hipotesis dengan menggunakan uji-t yang diperoleh thitung > ttabel yaitu 17,51 > 1,68023artinya Ho ditolak dan H1 diterima. Kesimpulan yang diperoleh pada penelitian ini adalah model learning cycle memiliki pengaruh yang signifikan terhadap keterampilan proses sains siswa pada mata pelajaran IPA kelas III.

This research is intended to know the influence of the use of learning cycle model to science process skill of third grade students of SD Negeri Harja Mekar 03 Kec. Cikarang. This research uses quantitative approach, with quasi experiment research method and research design nonequivalent control group design. The sampling technique used purposive sampling technique. The population used was 44 students with the total sample class of 23 students and control class of 21 students. The analysis technique used is t-test with significant level of 0.05. The result of the research shows that the mean value of posttest of experimental class is 82,17 higher than the mean of posttest of control class is 79,38. The result of hypothesis testing by using t-test obtained thitung> ttable that is 17,51> 1,68023artinya Ho rejected and H1 accepted. The conclusions obtained in this study is the learning cycle model has a significant influence on the science process skills of students on science subjects class III.
EVALUASI PEMBELAJARAN BERBASIS ISLAM KEMUHAMMADIYAHAN SEBAGAI INTERNALISASI PENGUATAN PENDIDIKAN KARAKTER SISWA DI SD MUHAMMADIYAH KOTA MALANG
Authors: Amelia Delora Jantung 
Published: October 2018

Fakta di lapangan memberikan gambaran bahwa terdapat praktik-praktik pendidikan di saat ini mengalami perubahan. Perubahan yang dimaksud adalah implementasi pembelajaran dengan mengintegrasikan pendidikan karakter. Pendidikan karakter bermuara pada praktik teladan dari setiap stake holder yang ada di sekolah. Stake holder memiliki kontribusi yang besar tidak hanya sebagi praktisi pendidikan, tetapi juga sebagai ujung tombak keberhasilan penerapan pendidikan karakter bagi siswa. Penelitian ini mendeskripsikan pelaksanaan evaluasi pembelajaran berbasis islam kemuhamadiyahan sebagai upaya penguatan pendidikan karakter di sekolah. Prosedur penelitian ini yaitu dengan cara mengamati kegiatan guru dan siswa saat pembelajaran berlangsung kemudian menganalisis apakah pelaksanan evaluasi dalam pembelajaran berbasis kemuhamamadiyahan mampu menumbuhkan pendidikan karakter siswa. Sebagian besar kegiatan implementasi kegiatan pembelajran islam kemuahmmadiyahan di SD Muhammadiyah 9 malang, sudah sesuai dengan standar implementasi kurikluum 2013. Pelakanaan implementasi kegiatan pembelajaran islam kemuhammadiyaan memiliki factor pendukung dan penghambat yang mana semua factor penghambat dapat di atasi dengan baik.
KUALITAS MEDIA CARD DANCE UNTUK PEMBELAJARAN SENI TARI DI LEMBAGA PENDIDIKAN
Authors: Rahma Pratiwi Emy Yunita 
Published: October 2018

The learning goal of card dance art for students is to re-express their past experience creatively, foster and develop creativitythat is expressed in the form of creative dance artwork. This study aims to determine the quality of card dance instructional media. The instructional media of dance art used is "Card Dance", which is a card with some pictures of Indonesian dance.This research used 4-D development model (Four D Models) from Thiagarajan and the research design is qualitative descriptive with discussion of the researchresult. The data collection results from the team validator will be analyzed descriptively and quantitatively. This research aims to provide benefits to the improvement of card dance media for learning dance art in educational institution.Based on the result of the card dance mediaquality to teach dance art at educational institution, and analyzed from several aspects of the quality of material and media, it shows the total score of 109 out of a maximum score of 148. After being analyzed and presented, the results obtained a result of 74.5%. Overall, the instructional media of card dance can be defined as a good quality instructional media and deserve to be used to support learning.
PENGEMBANGAN PERANGKAT PEMBELAJARAN DENGAN PENDEKATAN CONTEXTUAL TEACHING AND LEARNING UNTUK MENINGKATKAN MOTIVASI DAN HASIL BELAJAR BAGI SISWA KELAS IV SEKOLAH DASAR
Authors: Nugroho Rizky 
Published: October 2018

Penelitian ini bertujuan untuk menghasilkan perangkat pembelajaran dengan menggunakan pendekatan Contextual Teaching and Learning (CTL) yang valid, praktis, dan efektif bagi siswa kelas IV SD. Penelitian ini merupakan penelitian pengembangan dengan menggunakan model pengembangan 4D yang meliputi empat tahap mendefinisikan, mendesain, mengembangkan dan menyebarluaskan. Hasil penelitian menunjukkan bahwa perangkat pembelajaran dinyatakan valid dengan kategori sangat baik oleh validator. Penerapan perangkat pembelajaran dapat terlaksana dengan kategori sangat baik dan respon siswa terhadap perangkat pembelajaran berada pada kategori sangat baik. Terdapat perbedaan motivasi belajar dan hasil belajar yang signifikan antara kelas eksperimen yang menggunakan perangkat pembelajaran dengan pendekatan Contextual Teaching and Learning dan kelas kontrol yang tidak menggunakan perangkat pembelajaran hasil pengembangan. Berdasarkan hasil penelitian, dapat disimpulkan bahwa pengembangan perangkat pembelajaran dengan menggunkan pendekatan Contextual Teaching and Learning memenuhi kriteria valid, praktis, dan efektif untuk meningkatkan motivasi dan hasil belajar bagi siswa kelas IV SD.
MEDIA PUZZLE SOGAM (SOAL DAN GAMBAR) PADA MATERI PENJUMLAHAN DAN PENGURANGAN BILANGAN
Authors: Sri Nyamik 
Published: October 2018

Media puzzle SOGAM merupakan pengembangan puzzle dengan menggunakan 2 sisi gambar. Bagian depan terdapat latihan soal dan pasangan jawaban, bagian belakang terdapat gambar hewan/tumbuhan yang berfungsi untuk menilai kebenaran jawaban bagaian depan. Tujuan penelitian ini adalah mendeskripsikan proses dan menghasilkan produk media puzzle SOGAM pada materi penjumlahan dan pengurangan bilangan. Pengembangan pada penelitian ini menggunakan model pengembangan ADDIE (Analysis, Design, Development, Implementation, and Evaluation). Uji coba produk dilakukan dengan uji coba terbatas dan uji coba lapangan lebih luas dengan subjek uji coba peserta didik kelas 1 SD Kartika IV-I Malang. Instrument yang digunakan adalah angket dan tes. Media puzzle SOGAM dinyatakan layak berdasarkan uji kelayakan yang diperoleh dari ahli media dengan presentase total 93,7 %, dan ahli materi dengan presentase total 85,3%. Uji kepraktisan media puzzle SOGAM diperoleh dari angket respon calon pengguna (mahasiswa) dengan presentase total 96,8%, angket respon guru dengan presentase total 100% dan angket respon siswa dengan presentase total 100%. Media puzzle SOGAM telah teruji keefektifannya hasil pretest dan postest dengan presentase pretest sebesar 80% dan postest sebesar 90%.
PERBEDAAN HASIL BELAJAR DALAM PEMBELAJARAN YANG MENGGUNAKAN DAN TIDAK MENGGUNAKAN TEKNIK PEMBELAJARAN PROBING PROMPTING
Authors: Sulistyowati Prihatin 
Published: October 2018

Penelitian bertujuan untuk mengetahui perbedaan hasil belajar pembelajaran yang menggunakan dan tidak menggunakan teknik pembelajaran probing prompting di kelas V SDN Tanjungrejo 01. Penelitian menggunakan Quasy Eksperimental Design. Populasi dari penelitian ini adalah seluruh siswa kelas V. Analisis data menggunakan analisis data kuantitatif. Uji analisis data meliputi: uji instrumen, uji prasyarat analisis, dan uji hipotesis. Hasil penelitian menunjukkan bahwa: (1) hasil belajar peserta didik kelas eksperimen mempunyai nilai rata-rata (84,53). (2) hasil belajar peserta didik kelas kontrol mempunyai nilai rata-rata (64,22). Hal tersebut dibuktikan dengan menggunakan uji independent sample t-test yang menunjukkan jika dilihat dari signifikan lebih kecil dari 0,05, yaitu 0,00. (0,05>0,00) jadi pada penelitian ini dapat disimpulkan bahwa ada perbedaan hasil belajar pembelajaran yang menggunakan dan tidak menggunakan teknik pembelajaran probing prompting.
Perbedaan Strategi Talking Stick dan Numbered Heads Together (NHT) pada Mata Pelajaran IPS
Authors: Sakdiyah Siti Halimatus , Malik Jamil Achmad Maulana , Harun Harun 
Published: October 2018

Artikel ini bertujuan untuk mengetahui perbedaan dari hasil belajar siswa yang diajar menggunakan model pembelajaran Talking Stick dan model Numbered Head Together (NHT) siswa kelas IV. Hal ini dilakukan karena proses pembelajaran IPS dikelas IV SDN Gadang 4 Malang masih menggunakan metode ceramah, hafalan dan pemberian tugas, dimana guru menerangkan sedangkan siswa mendengarkan, mencatat, dan mengerjakan tugas. Keterlibatan siswa disini adalah keterlibatan pasif, komunikasi terjalin satu arah, hal ini yang menyebabkan hasil belajar siswa rendah.
Penelitian ini dirancang menggunakan Quasi Experiment. Dengan desain penelitian Nonequivalen Control Group Design. Variabel bebasnya model pembelajaran Talking Stick dan model pembelajaran Numbered Head Together (NHT). Variabel terikatnya hasil belajar IPS. Populasi penelitian ini adalah seluruh siswa kelas IV SDN Gadang 4 Malang, dengan jumlah 44 siswa. Pengambilan sampling pada penelitian ini menggunakan teknik sampling jenuh. Kelas IVA sebagai kelas eksperimen 1 yang berjumlah 22 siswa dan kelas IVB sebagai kelas eksperimen 2 yang berjumlah 22 siswa. Untuk mengumpulkan data tersebut diperlukan tes dan uji hipotesisnya menggunakan Uji t.
Hasil penelitian menunjukkan bahwa : ada perbedaan hasil belajar siswa menggunakan model pembelajaran Talking Stick dan Numbered Heads Together (NHT) pada pelajaran IPS kelas IV SDN Gadang 4 Malang, dengan nilai signifikansi 0,035 < 0,05 dan Fhitung sebesar 0,264. Jika sig > 0,05 maka Ho = diterima , dan jika sig < 0,05 maka Ho = ditolak. Artinya bahwa Ho ditolak dan H1 diterima. Jika Ho ditolak maka ada perbedaan hasil belajar IPS antara siswa yang diajar menggunakan model Talking Stick dengan siswa yang diajar menggunakan model Numbered Head Together (NHT). Sehingga dapat disimpulkan bahwa ada perbedaan prestasi belajar siswa menggunakan model pembelajaran Talking Stick dan Numbered Heads Together (NHT) pada pelajaran IPS kelas IV SD Negeri 1 Brongkal Kecamatan Pagelaran Kabupaten Malang.
Nilai-Nilai Prososial Dalam Kaulinan Oray-Orayan Di Sekolah Dasar Negeri Isola Kota Bandung
Authors: Istianti Tuti 
Published: October 2018

The verbal and gestures values disclosure of west java traditional play named “Kaulinan Orayorayan” is needed as a starting poin in improving learning at schools. The article explores and identifies the values of pro-social of the physical and phsychological activities of “Kaulinan Oray-orayan” in which etnopedagogically is rich of cultural and national values. Based on semiotics study and descriptive analysis, it is identified the pro-social values in “Kaulinan Orayorayan” such as getting fun, entertaining, helping, building friendship, sacrificing, generousity, and sharing. It is recommended that the valeus of “Kaulinan Orayorayan” should be applied and improved in the teaching and learning practices at schools.
Penanaman Nilai-Nilai Moral Pada Pembelajaran Di Taman Kanak-Kanak
Authors: Hermuttaqien Bhakti Prima Findiga , Mutatik Mutatik 
Published: October 2018

Latar belakang penelitian ini adalah penurunan nilai-nilai moral generasi muda. Indikasi penurunan nilai-nilai moral tersebut yaitu semakin maraknya tindakan kriminal, turunnya rasa hormat dan sopan santun anak terhadap guru ataupun orang lain. Hal ini dikarenakan minimnya pendidikan nilai-nilai moral pada anak usia dini melalui kegiatan pembelajaran di Taman Kanak-kanak. Tujuan dari penelitian ini untuk menanamkan nilai-nilai moral pada pembelajaran di Taman Kanak-kanak. Penelitian ini merupakan penelitian kualitatif dengan teknik penelitian studi kasus. Penelitian dilakukan di TK Dharma Wanita Sumberporong. pengumpulan data dilakukan dengan mengadakan pengamatan, wawancara mendalam, dan dokumentasi. Uji keabsahan data dilakukan dengan menggunakan triangulasi sumber dan teknik. Hasil penelitian menunjukan: (1) Nilai-nilai moral yang ditanamkan di TK Dharma Wanita melalui kegiatan pembelajaran diantaranya adalah: tolong menolong, saling memaafkan, kasih sayang, berbagi kepada teman sebaya, menabung, sopan santun, menghormati dan menghargai orang lain.(2) Metode pembelajaran yang dilakukan untuk menanamkan nilai-nilai moral adalah keteladanan, pembiasaan, stimulus, bermain, bernyanyi, pemberian reward, pemberian nasihat.(3) Faktor penghambat dalam menanamkan nilai-nilai moral diantaranya yaitu: kurangnya tenaga pendidik, kurangnya penguasaan psikologi anak, guru masih mengalami kesulitan dalam menangani anak-anak yang nakal, lingkungan yang kurang kondusif. Faktor pendukung yaitu: berbagai media belajar seperti: alat bermain, CD, buku serta dukungan moral dari para guru.