bluuebunny/crossref_metadata_2025_split huggingface dataset:
Loss of Fas Expression and Function Is Coupled with Colon Cancer Resistance to Immune Checkpoint Inhibitor Immunotherapy
Authors: Xiao Wei , Ibrahim Mohammed L. , Redd Priscilla S. , Klement John D. , Lu Chunwan , Yang Dafeng , Savage Natasha M. , Liu Kebin 
Published: November 2018

Abstract

Despite the remarkable efficacy of immune checkpoint inhibitor (ICI) immunotherapy in various types of human cancers, colon cancer, except for the approximately 4% microsatellite-instable (MSI) colon cancer, does not respond to ICI immunotherapy. ICI acts through activating CTLs that use the Fas–FasL pathway as one of the two effector mechanisms to suppress tumor. Cancer stem cells are often associated with resistance to therapy including immunotherapy, but the functions of Fas in colon cancer apoptosis and colon cancer stem cells are currently conflicting and highly debated. We report here that decreased Fas expression is coupled with a subset of CD133+CD24lo colon cancer cells in vitro and in vivo. Consistent of the lower Fas expression level, this subset of CD133+CD24loFaslo colon cancer cells exhibits decreased sensitivity to FasL-induced apoptosis. Furthermore, FasL selectively enriches CD133+CD24loFaslo colon cancer cells. CD133+CD24loFaslo colon cancer cells exhibit increased lung colonization potential in experimental metastatic mouse models and decreased sensitivity to tumor-specific CTL adoptive transfer and ICI immunotherapies. Interestingly, FasL challenge selectively enriched this subset of colon cancer cells in microsatellite-stable (MSS) but not in the MSI human colon cancer cell lines. Consistent with the downregulation of Fas expression in CD133+CD24lo cells, lower Fas expression level is significantly correlated with decreased survival in patients with human colon cancer.

Implications:
Our data determine that CD133+CD24loFaslo colon cancer cells are capable to evade Fas-FasL cytotoxicity of tumor-reactive CTLs and targeting this subset of colon cancer cells is potentially an effective approach to suppress colon cancer immune evasion.
A General Description of Entrainment in Buoyant Cloudy Plumes Including the Effects of Mixing-Induced Evaporation
Authors: Savre Julien , Herzog Michael 
Published: November 2018

AbstractIn this work, the steady-state one-dimensional axisymmetric plume theory is revisited and generalized to include the effects of nonhomogeneous updraft velocity and buoyancy profiles across the plume, environmental shear, and, more importantly, evaporative cooling resulting from the mixing between cloudy air and the dry environment. Based on an energy consistency argument, a method is proposed to derive a relationship for the fractional lateral mixing rate (which may here be positive or negative) from the plume’s integral equations, as well as a set of equations for the equivalent plume properties, both of which maintain a high degree of generality by incorporating effects of environmental shear and inhomogeneous radial distributions. In the absence of wind shear, a simpler entrainment-rate closure is proposed, which is then further constrained by systematically varying the plume and environmental conditions and allowing evaporative cooling to occur. The fractional mixing rate is shown to be strongly correlated with the plume buoyancy and, to a lesser extent, to the critical mixing fraction (i.e., the fraction of dry air that needs to be mixed with cloudy air to make the mixture neutrally buoyant). Quantitative estimates of this dependency are given to facilitate implementations of the new model in convection parameterizations. Analyzing the proposed closure suggests that it could capture features observed in recent high-resolution simulations and that it is consistent with the buoyancy-sorting concept. The results therefore support recent findings concerning the parameterization of entrainment for moist atmospheric convection.
An Improved Double-Gaussian Closure for the Subgrid Vertical Velocity Probability Distribution Function
Authors: Fitch A. C. 
Published: November 2018

Abstract
The vertical velocity probability distribution function (PDF) is analyzed throughout the depth of the lower atmosphere, including the subcloud and cloud layers, in four large-eddy simulation (LES) cases of shallow cumulus and stratocumulus. Double-Gaussian PDF closures are examined to test their ability to represent a wide range of turbulence statistics, from stratocumulus cloud layers characterized by Gaussian turbulence to shallow cumulus cloud layers displaying strongly non-Gaussian turbulence statistics. While the majority of the model closures are found to perform well in the former case, the latter presents a considerable challenge. A new model closure is suggested that accounts for high skewness and kurtosis seen in shallow cumulus cloud layers. The well-established parabolic relationship between skewness and kurtosis is examined, with results in agreement with previous studies for the subcloud layer. In cumulus cloud layers, however, a modified relationship is necessary to improve performance. The new closure significantly improves the estimation of the vertical velocity PDF for shallow cumulus cloud layers, in addition to performing well for stratocumulus. In particular, the long updraft tail representing the bulk of cloudy points is much better represented and higher-order moments diagnosed from the PDF are also greatly improved. However, some deficiencies remain owing to fundamental limitations of representing highly non-Gaussian turbulence statistics with a double-Gaussian PDF.
Decadal Transition of the Leading Mode of Interannual Moisture Circulation over East Asia–Western North Pacific: Bonding to Different Evolution of ENSO
Authors: Li Xiuzhen , Wen Zhiping , Chen Deliang , Chen Zesheng 
Published: November 2018

AbstractThe El Niño–Southern Oscillation (ENSO) cycle has a great impact on the summer moisture circulation over East Asia (EA) and the western North Pacific [WNP (EA-WNP)] on an interannual time scale, and its modulation is mainly embedded in the leading mode. In contrast to the stable influence of the mature phase of ENSO, the impact of synchronous eastern Pacific sea surface temperature anomalies (SSTAs) on summer moisture circulation is negligible during the 1970s–80s, while it intensifies after 1991. In response, the interannual variation of moisture circulation exhibits a much more widespread anticyclonic/cyclonic pattern over the subtropical WNP and a weaker counterpart to the north after 1991. Abnormal moisture moves farther northward with the enhanced moisture convergence, and thus precipitation shifts from the Yangtze River to the Huai River valley. The decadal shift in the modulation of ENSO on moisture circulation arises from a more rapid evolution of the bonding ENSO cycle and its stronger coupling with circulation over the Indian Ocean after 1991. The rapid development of cooling SSTAs over the central-eastern Pacific, and warming SSTAs to the west over the eastern Indian Ocean–Maritime Continent (EIO-MC) in summer, stimulates abnormal descending motion over the western-central Pacific and ascending motion over the EIO-MC. The former excites an anticyclone over the WNP as a Rossby wave response, sustaining and intensifying the WNP anticyclone; the latter helps anchor the anticyclone over the tropical–subtropical WNP via an abnormal southwest–northeast vertical circulation between EIO-MC and WNP.
Moored Observations of the Surface Meteorology and Air–Sea Fluxes in the Northern Bay of Bengal in 2015
Authors: Weller R. A. , Farrar J. T. , Seo Hyodae , Prend Channing , Sengupta Debasis , Lekha J. Sree , Ravichandran M. , Venkatesen R. 
Published: November 2018

AbstractTime series of surface meteorology and air–sea fluxes from the northern Bay of Bengal are analyzed, quantifying annual and seasonal means, variability, and the potential for surface fluxes to contribute significantly to variability in surface temperature and salinity. Strong signals were associated with solar insolation and its modulation by cloud cover, and, in the 5- to 50-day range, with intraseasonal oscillations (ISOs). The northeast (NE) monsoon (DJF) was typically cloud free, with strong latent heat loss and several moderate wind events, and had the only seasonal mean ocean heat loss. The spring intermonsoon (MAM) was cloud free and had light winds and the strongest ocean heating. Strong ISOs and Tropical Cyclone Komen were seen in the southwest (SW) monsoon (JJA), when 65% of the 2.2-m total rain fell, and oceanic mean heating was small. The fall intermonsoon (SON) initially had moderate convective systems and mean ocean heating, with a transition to drier winds and mean ocean heat loss in the last month. Observed surface freshwater flux applied to a layer of the observed thickness produced drops in salinity with timing and magnitude similar to the initial drops in salinity in the summer monsoon, but did not reproduce the salinity variability of the fall intermonsoon. Observed surface heat flux has the potential to cause the temperature trends of the different seasons, but uncertainty in how shortwave radiation is absorbed in the upper ocean limits quantifying the role of surface forcing in the evolution of mixed layer temperature.
Baroclinic Tidal Sea Level from Exact-Repeat Mission Altimetry
Authors: Zaron Edward D. 
Published: November 2018

AbstractA near-global model for the sea surface expression of the baroclinic tide has been developed using exact-repeat mission altimetry. The methodology used differs in detail from other altimetry-based estimates of the open ocean baroclinic tide, but it leads to estimates that are broadly similar to previous results. It may be used for prediction of the baroclinic sea level anomaly at the frequencies of the main diurnal and semidiurnal tides , , , and , as well as the annual modulates of , denoted and . The tidal predictions are validated by computing variance reduction statistics using independent sea surface height data from the CryoSat-2 altimeter mission. Typical midocean baroclinic tidal signals range from a few millimeters to centimeters of elevation, corresponding to subsurface isopycnal displacements of tens of meters; however, in a few regions, larger signals are present, and it is found that the present model can explain more than 13-cm2 variance at some sites. The predicted tides are also validated by comparison with a database of hourly currents inferred from drogued surface drifters. The database is large enough to permit assessment of a simple model for scattering of the low-mode tide. Results indicate a scattering time scale of approximately 1 day, consistent with a priori estimates of time-variable refraction by the mesoscale circulation.
An Integrated Approach to Weather Radar Calibration and Monitoring Using Ground Clutter and Satellite Comparisons
Authors: Louf Valentin , Protat Alain , Warren Robert A. , Collis Scott M. , Wolff David B. , Raunyiar Surendra , Jakob Christian , Petersen Walter A. 
Published: November 2018

AbstractThe stability and accuracy of weather radar reflectivity calibration are imperative for quantitative applications, such as rainfall estimation, severe weather monitoring and nowcasting, and assimilation in numerical weather prediction models. Various radar calibration and monitoring techniques have been developed, but only recently have integrated approaches been proposed, that is, using different calibration techniques in combination. In this paper the following three techniques are used: 1) ground clutter monitoring, 2) comparisons with spaceborne radars, and 3) the self-consistency of polarimetric variables. These techniques are applied to a C-band polarimetric radar (CPOL) located in the Australian tropics since 1998. The ground clutter monitoring technique is applied to each radar volumetric scan and provides a means to reliably detect changes in calibration, relative to a baseline. It is remarkably stable to within a standard deviation of 0.1 dB. To obtain an absolute calibration value, CPOL observations are compared to spaceborne radars on board TRMM and GPM using a volume-matching technique. Using an iterative procedure and stable calibration periods identified by the ground echoes technique, we improve the accuracy of this technique to about 1 dB. Finally, we review the self-consistency technique and constrain its assumptions using results from the hybrid TRMM–GPM and ground echo technique. Small changes in the self-consistency parameterization can lead to 5 dB of variation in the reflectivity calibration. We find that the drop-shape model of Brandes et al. with a standard deviation of the canting angle of 12° best matches our dataset.
Analytical performances of PENIA and PETIA urinary cystatin C determination allow tubular injury investigation
Authors: Bargnoux Anne-Sophie , Azoury Vincent , Badiou Stéphanie , Klouche Kada , Plawecki Maëlle , Kuster Nils ,   Anne-Marie-Dupuy, Cristol Jean-Paul 
Published: November 2018

Background The study was designed to evaluate the analytical performances of two ERM-DA471/IFCC traceable cystatin C (CysC) reagents available on the market for urinary CysC (u-CysC) quantification. In addition, clinical relevance was assessed by measuring u-CysC in healthy controls and in patients with tubular dysfunction. Methods CysC in urine was measured by a particle-enhanced nephelometric immunoassay using Siemens reagents and by a particle-enhanced turbidimetric immunoassay using DiaSys reagents. Imprecision, linearity, limit of detection and limit of quantification were evaluated according to CLSI recommendations. The two methods were tested on 150 urinary samples from 50 healthy subjects, 50 HIV patients with tubular dysfunction and 50 patients who developed acute kidney acute injury. Results Within-laboratory coefficients of variations were below 4%. The lower limit of quantification of the assay was found at 0.043 and 0.046 mg/L for DiaSys and Siemens, respectively. The following Passing-Bablok regression equations were obtained: DiaSys = 0.99 Siemens + 0.00. Using Bland-Altman analysis, the mean bias was –0.004 mg/L on the analytical range between 0.02 and 1 mg/L. Median u-CysC in 50 HIV patients with tubular dysfunction and in 50 patients with AKI was higher than in control subjects. Conclusions Both Siemens and DiaSys reagents demonstrated reliable and reproducible performances allowing easy determination of u-CysC on automated platforms in clinical practice with potential interest for the detection of tubular dysfunction.
Treating the troponin: adverse consequences of over-treatment of elevated troponin in non-coronary presentations
Authors: Morrow A , Ahmad F , Steele C , McEntegart M , Murdoch D 
Published: November 2018

Background and aims Anti-platelet and anti-thrombotic therapy are well-established treatments in acute coronary syndromes. Highly sensitive assays have diminished the positive predictive value of troponin in acute coronary syndromes and increased the importance of the clinical assessment in interpreting positive results. This cohort study sought to investigate over-treatment of non-coronary troponin rises and associated adverse outcomes. Methods and results We reviewed 223 consecutive patients presenting to Queen Elizabeth University Hospital, Glasgow, with suspected acute coronary syndromes over a six-week period. Of these, 27 (12%) met our ‘inappropriate therapy’ criteria. This group had a low ischaemic risk (HEART score: 4.2 ± 1.4) (GRACE score: 117 ± 30.8) but an intermediate-high bleeding risk (CRUSADE score: 34 ± 14.5). Approximately half of the patients (14/27, 52%) reported chest pain, with only 4/27 (15%) having ischaemic ECG changes. There were three intracranial haemorrhages, each after the patient had received a single dose of aspirin, ticagrelor and fondaparinux. Conclusion The combination of injudicious high-sensitivity troponin testing with potent anti-platelet and anti-thrombotic therapy was associated with possible over-treatment of patients and associated harm. Emphasis on interpretation of troponin in the context of clinical presentation and improved awareness of type 2 myocardial infarction are essential to limit iatrogenic pharmacological harm.
Deep learning algorithms to identify documentation of serious illness conversations during intensive care unit admissions
Authors: Chan Alex , Chien Isabel , Moseley Edward , Salman Saad , Kaminer Bourland Sarah , Lamas Daniela , Walling Anne M , Tulsky James A , Lindvall Charlotta 
Published: November 2018

Background: Timely documentation of care preferences is an endorsed quality indicator for seriously ill patients admitted to intensive care units. Clinicians document their conversations about these preferences as unstructured free text in clinical notes from electronic health records. Aim: To apply deep learning algorithms for automated identification of serious illness conversations documented in physician notes during intensive care unit admissions. Design: Using a retrospective dataset of physician notes, clinicians annotated all text documenting patient care preferences (goals of care or code status limitations), communication with family, and full code status. Clinician-coded text was used to train algorithms to identify documentation and to validate algorithms. The validated algorithms were deployed to assess the percentage of intensive care unit admissions of patients aged ⩾75 that had care preferences documented within the first 48 h. Setting/participants: Patients admitted to one of five intensive care units. Results: Algorithm performance was calculated by comparing machine-identified documentation to clinician-coded documentation. For detecting care preference documentation at the note level, the algorithm had F1-score of 0.92 (95% confidence interval, 0.89 to 0.95), sensitivity of 93.5% (95% confidence interval, 90.0% to 98.0%), and specificity of 91.0% (95% confidence interval, 86.4% to 95.3%). Applied to 1350 admissions of patients aged ⩾75, we found that 64.7% of patient intensive care unit admissions had care preferences documented within the first 48 h. Conclusion: Deep learning algorithms identified patient care preference documentation with sensitivity and specificity approaching that of clinicians and computed in a tiny fraction of time. Future research should determine the generalizability of these methods in multiple healthcare systems.
Log-transformed B-type natriuretic peptide as a prognostic predictor in patients undergoing cardiovascular surgery
Authors: Takagi Sho , Machida Yuichiro , Kobata Takashi , Sakamoto Daisuke , Sakamoto Shigeru , Kanda Tsugiyasu 
Published: November 2018

Objective This study was performed to explore the association between circulating B-type natriuretic peptide (BNP) and other mortality-related factors in patients undergoing cardiovascular surgery. Methods In this observational study, multilevel linear regression analysis and multilevel survival analysis were performed to measure the log-transformed BNP (lnBNP) value at four time points in 197 patients with 788 repeated data measurements. Effects of the interaction between the time points and the two intervention groups (cardiac surgery and vascular surgery) were also investigated. Six models were evaluated to identify the best fit for the data. Stata/MP® version 14.2 (Stata Corp., College Station, TX, USA) was used to analyze the two-level variance component model fitting. Results There were significant differences in the fixed-effect parameters of lnBNP, such as the time point, age, body mass index, emergency operation, prognostic nutritional index, and estimated glomerular filtration rate. According to the multilevel survival analysis for all-cause death and vascular death, lnBNP significantly differed and was a common prognostic marker. Conclusion As lnBNP increased by 1 point, all-cause death increased 2.07 times and vascular death increased 3.10 times. lnBNP is an important prognostic predictor and quantitative biochemical marker in patients undergoing cardiovascular surgery.
Evaluation of the bioactivity about anti-sca-1/basic fibroblast growth factor-urinary bladder matrix scaffold for pelvic reconstruction
Authors: Li Jiankui , Chen Xi , Ling Kaijian , Liang Zhiqing , Xu Huicheng 
Published: November 2018

Introduction and hypothesis: Pelvic support structure injury is the major cause of pelvic organ prolapse. At present, polypropylene-based filler material has been suggested as a common method to treat pelvic organ prolapse. However, it cannot functionally rehabilitate the pelvic support structure. In addition to its poor long-term efficiency, the urinary bladder matrix was the most suitable biological scaffold material for pelvic floor repair. Here, we hypothesize that anti-sca-1 monoclonal antibody and basic fibroblast growth factor were cross-linked to urinary bladder matrix to construct a two-factor bioscaffold for pelvic reconstruction. Methods Through a bispecific cross-linking reagent, sulfosuccinimidyl 4-[N-maleimidomethyl] cyclohexane-1-carboxylate (sulfo-smcc) immobilized anti-sca-1 and basic fibroblast growth factor to urinary bladder matrix. Then scanning electron microscope and plate reader were used to detect whether the anti-sca-1/basic fibroblast growth factor-urinary bladder matrix scaffold was built successfully. After that, the capacity of enriching sca-1 positive cells was measured both in vitro and in vivo. In addition, we evaluated the differentiation capacity and biocompatibility of the scaffold. Finally, western blotting was used to detect the level of fibulin-5 protein. Results The scanning electron microscope and plate reader revealed that the double-factor biological scaffold was built successfully. The scaffold could significantly enrich a large number of sca-1 positive cells both in vitro and in vivo, and obviously accelerate cells and differentiate functional tissue with good biocompatibility. Moreover, the western blotting showed that the scaffold could improve the expression of fibulin-5 protein. Conclusion The anti-sca-1/basic fibroblast growth factor-urinary bladder matrix scaffold revealed good biological properties and might serve as an ideal scaffold for pelvic reconstruction.
From crisis to crisis: Capitalism, chaos and constant unpredictability
Authors: Chowdhury Anis , Żuk Piotr 
Published: November 2018

AbstractFar from being an event of a decade ago, the 2008 global financial crisis is a manifestation of an ongoing crisis of the world order, with social, political and ecological dimensions that cannot be seen separately from each other. The root cause of the crisis can be traced back to the collapse of the Bretton Woods System in August 1971, and the failure to design an equitable and inclusive global financial and economic governance architecture consistent with the changed global economic realities. The vacuum was quickly taken up by the neoliberal orthodoxy that pushed the agenda of wholesale liberalisation, resulting in unprecedented domination of speculative finance capital and multinational corporation–led globalisation. This has seen falling share of wages in national income, growing wealth concentration, rising income inequality and ballooning of household debts. The consequence was frequent and increasingly deeper and wider financial crises.