bluuebunny/crossref_metadata_2025_split huggingface dataset:
BRAFV600E mutations and immunohistochemical expression of VE1 protein in low‐grade serous neoplasms of the ovary
Authors: Turashvili Gulisa , Grisham Rachel N , Chiang Sarah , DeLair Deborah F , Park Kay J , Soslow Robert A , Murali Rajmohan 
Published: May 2018

AimsThe most common BRAF mutation in ovarian low‐grade serous neoplasms (LGSNs) involves substitution of valine by glutamic acid at position 600 (V600E). Small studies have demonstrated high specificity of immunohistochemistry with mutation‐specific monoclonal antibody VE1. We sought to investigate the expression of VE1 protein in LGSNs and its correlation with BRAF mutation‐associated histological features and BRAF mutation status.Methods and resultsWe reviewed pathology reports and available slides from ovarian serous borderline tumours (SBTs) and low‐grade serous carcinomas (LGSCs) diagnosed between 2000 and 2012. VE1 immunohistochemistry was performed on formalin‐fixed, paraffin‐embedded tissue sections. Tumours with ≥50% positive cells were considered positive. Of 121 LGSNs, there were 73 SBTs, eight SBTs with micropapillary features (mpSBT) and 40 LGSCs (22 primary, 18 metastatic). VE1 was positive in 52% (38 of 73) of SBTs and 9% (two of 22) of primary LGSCs, and in none of the mpSBTs and metastatic LGSCs (P < 0.0001). Of 76 tumours with known mutation status, 42 (55%) harboured mutations, including BRAFV600E (26, 34%), KRASG12D (eight, 11%), and KRASG12V (eight, 11%). BRAFV600E mutations were present in 48% (25 of 52) of SBTs and 5% (one of 22) of LGSCs (P < 0.0001). VE1 was positive in 96% (25 of 26) of BRAFV600E‐mutated tumours and correlated with BRAF mutation‐associated histological features (P < 0.0001).ConclusionsBRAFV600E mutations are significantly more common in SBTs than in LGSCs. Immunohistochemical expression of VE1 protein is associated strongly with BRAFV600E mutation and BRAF mutation‐associated histological features. VE1 immunohistochemistry is a reliable method for the detection of BRAFV600E mutations.
The Earth's natural pulse electromagnetic fields for earthquake time–frequency characteristics: Insights from the EEMD–WVD method
Authors: Hao Guo‐Cheng , Bai Yu‐Xiao , Liu Hui , Zhao Juan , Zeng Zuo‐Xun 
Published: May 2018

AbstractMonitoring the Earth's natural pulse electromagnetic field (ENPEMF) signal is a geomagnetic detection approach for the Earth internal and external studies, and it provides a reference for earthquake prediction. In this paper, the Ensemble Empirical Mode Decomposition–Wigner Ville Distribution (EEMD–WVD) method is used to process the time sequences of the ENPEMF signal detected by GR‐01 receiving devices during the Lushan MS7.0 earthquake (April 20th, 2013). By obtaining the intrinsic mode function (IMF), the time–frequency–amplitude joint distribution and the energy spectrum of the ENPEMF signal, the suspected anomalies before the earthquake could be shown. The results illustrate the ENPEMF signal before an earthquake and its time–frequency characteristics based on an EEMD–WVD algorithm, proving useful information about the anomalies around the time of the earthquake.
Age‐related changes in the gut microbiota of wild House Sparrow nestlings
Authors: Kohl Kevin D. , Brun Antonio , Caviedes‐Vidal Enrique , Karasov William H. 
Published: May 2018

Here, we document the development of the House Sparrow Passer domesticus gut microbiota for the benefit of future ecological and evolutionary studies in this widely used avian system. We collected and inventoried luminal and mucosal samples from the distal guts of nestling House Sparrows between 3 and 12 days of age, and also collected similar samples from adult birds. Luminal bacterial community membership and structure differed significantly between nestlings and adults. The relative abundance of Proteobacteria in the lumen decreased around day 9, whereas the relative abundance of Firmicutes increased, demonstrating age‐related changes in the microbiota of House Sparrows.
Hair colour and skin colour together influence perceptions of age, health and attractiveness in lightly pigmented young women
Authors: Fink B. , Liebner K. , Müller A.‐K. , Hirn T. , McKelvey G. , Lankhof J. 
Published: May 2018

AbstractObjectiveResearch documents that even subtle changes in visible skin condition affect perceptions of age, health and attractiveness. There is evidence that hair quality also affects the assessment of physical appearance, as variations in hair diameter, hair density and hairstyle have systematic effects on perception. Here, we consider combined effects of hair colour and skin colour on the perception of female physical appearance.MethodsIn two experiments, we digitally manipulated facial skin colour of lightly pigmented, young women, both between‐subjects (Experiment 1) and within‐subjects (Experiment 2), and investigated possible interactions with hair colour in regard to age, health, and attractiveness perception.ResultsIn both experiments, we detected hair colour and skin colour interaction effects on men's and women's assessments. For between‐subjects comparisons, participants with lighter hair colour were judged to be younger than those with darker shades; this effect was more pronounced in women with light skin colour. No such effect was observed for within‐subjects variation in skin colour. Both experiments showed that smaller perceived contrast between hair colour and skin colour resulted in more positive responses.ConclusionWe conclude that hair colour and facial skin colour together have an effect on perceptions of female age, health, and attractiveness in young women, and we discuss these findings with reference to the literature on the role of hair and skin in the assessment of female physical appearance.
Control of lactic acid bacteria in fermented beverages using lysozyme and nisin: test of traditional beverage boza as a model food system
Authors: Sozbilen Gozde Seval , Korel Figen , Yemenicioğlu Ahmet 
Published: May 2018

SummaryThe objective of this study was to increase quality and limited shelf‐life of boza (3–15 days), a traditional Balkan origin fermented beverage using lysozyme (LYS) and/or nisin (NIS). For this purpose, the effectiveness of NIS, LYS and LYS:NIS combinations was first tested in a broth medium at 4 °C for 3 weeks on Lactobacillus plantarum, one of the frequently isolated lactic acid bacteria in boza. Stability of LYS and NIS in boza, their effects on LAB counts, and chemical and sensory properties of boza were then evaluated during cold storage at 4 °C. Results of LAB counts as well as pH, d‐ and l‐lactic acid, and titratable acidity measurements showed that LAB in boza containing NIS (250 μg g−1) or LYS:NIS (500:250 μg g−1) could be controlled without reducing LAB counts below 6 log CFU mL−1 during 2 weeks shelf‐life. In contrast, LYS (500 μg g−1) alone could not control LAB in boza to delay its acidic spoilage. Positive effects of NIS and LYS:NIS application on quality of boza were also proved with sensory analysis by panelists and e‐nose measurements. This work showed that use of natural GRAS agents in preservation of fermented beverages containing probiotic LAB is possible without affecting their characteristic aroma and flavour.
The effect of health coaching on treatment adherence, self‐efficacy, and quality of life in patients with chronic obstructive pulmonary disease
Authors: Tülüce Derya , Kutlutürkan Sevinç 
Published: May 2018

AbstractAimThe aim of this study was to determine the impact of health coaching on respiratory functions, treatment adherence, self‐efficacy, and quality of life in chronic obstructive pulmonary disease patients.MethodsThis study was conducted as an experimental study with non‐randomized control group in chronic obstructive pulmonary disease individuals. The study group included a total of 54 chronic obstructive pulmonary disease patients (27 patients both health coaching and control groups), was selected between 13 July 2016 and 2 July 2017. Data were collected by using a questionnaire form, St. George Respiratory Questionnaire, 8‐Item Morisky Adherence Scale, and chronic obstructive pulmonary disease Self‐Efficacy Scale. A total of 12 coaching interviews were conducted with the patients in the health coaching group.ResultsAfter health coaching intervention, self‐efficacy scale general score, and St. George Respiratory Questionnaire, total scores were found statistically significant different between 2 groups in interaction values. There was a significant difference in the 8‐Item Morisky Adherence Scale scores for degree of treatment adherence between the groups.ConclusionThe health coaching intervention is recommended, as it contributes to facilitate the treatment adherence and increase the self‐efficacy skills by improving the quality of life of the chronic obstructive pulmonary disease patients.
The Syrian Refugee Crisis in the Kurdish Region of Iraq: Explaining the Role of Borders in Situations of Forced Displacement
Authors: Dionigi Filippo 
Published: May 2018

AbstractMore than 250,000 Syrian refugees have relocated in the Kurdish Region of Iraq since 2011. The local response has been relatively receptive towards them. This study proposes an analysis of the concept of border and applies the idea of “border thinness” as an interpretative framework to examine the nature of transborder relations between the Iraqi and Syrian Kurdish areas. Relying on secondary sources, technical and elite level interviews with social and political actors in KRI, the analysis highlights how Kurdish areas of Syria and Iraq have maintained transborder relations which shaped the dynamics of displacement from Syria and constituted the context for a relatively receptive stance towards Syrian Kurdish refugees. Borders do not always mark a sharp territorial separation between social, political, and economic realities; instead a more complex conceptualization exposes transborder relational spaces, explaining the modes of displacement and its responses from host communities.
Hidden care: Revelations of a case‐note audit of physical health care in a community mental health service
Authors: Lawn Sharon , Zabeen Sara , Rowlands Nikki , Picot Sharon 
Published: May 2018

AbstractPeople with severe mental illness (SMI) are widely reported to be at an increased risk of morbidity and premature death due to physical health conditions. Mental health nurses are ideally placed to address physical and mental health comorbidity as part of their day‐to‐day practice. This study involved an audit of hardcopy and electronic clinical case‐notes of a random sample of 100 people with SMI case managed by community mental health service in metropolitan South Australia, to determine how well physical health conditions and risk factors, screening, and follow‐up are recorded within their service records. Every contact between 1 July 2015 and 30 June 2016 was read. One‐way ANOVA, Scheffe's test, and Fisher's exact test determined any significant associations across audit variables, which included gender, age, income, living arrangement, diagnosis, lifestyle factors, recording of physical health measures, and carer status. A focus on physical health care was evident from everyday case‐note records; however, because this information was ‘buried’ within the plethora of entries and not brought to the fore with other key information about the person's psychiatric needs, it remained difficult to gain a full picture of potential gaps in physical health care for this population. Under‐reporting, gaps and inconsistencies in the systematic recording of physical health information for this population are likely to undermine the quality of care they receive from mental health services, the ability of mental health service providers to respond in a timely way to their physical healthcare needs, and their communication with other healthcare providers.
Therapeutic optimism and attitudes among medical and surgical nurses towards attempted suicide
Authors: Barnfield Jakqui , Cross Wendy , McCauley Kay 
Published: May 2018

AbstractIdentification of the attitudes to consumers admitted to hospital following a suicide attempt and the therapeutic optimism of nurses caring for this cohort is vital to ascertain the level of nursing care provided. A convenience sample of 72 Registered and enrolled nurses from a large metropolitan health service in South Eastern Melbourne, Australia, completed a demographic questionnaire, the Elsom Therapeutic Optimism Scale (ETOS), and the Attitudes to Attempted Suicide‐Questionnaire (ATAS‐Q). Data were analysed using SPSS (version 25). Whilst the ETOS & ATAS‐Q correlate positively, themes of shame, blame, misunderstandings, and myths about suicide influence nurses’ perspectives when providing suicide attempt aftercare. This may potentially lead to care that is tokenistic and task focused. To develop their professional skill set when providing suicide attempt aftercare, nurses need both formal and informal education and opportunities to reflect on their practice.
Microstructure and tribological behavior of TiAlSiN coatings deposited by deep oscillation magnetron sputtering
Authors: Ou Y. X. , Chen H. , Li Z. Y. , Lin J. , Pan W. , Lei M. K. 
Published: May 2018

AbstractDevelopment of pulsed‐techniques aimed to generate highly ionized target species and high plasma density opens up a new way to tailor composition, structure, and properties of coatings. In this work, TiAlSiN coatings have been deposited at various negative substrate biases (Vs) using deep oscillation magnetron sputtering by sputtering a TiAlSi compound target in Ar/N2 mixtures. The increase in Vs from −30 to −120 V resulted in a decrease in (111)‐preferred orientation and grain size, together with the increase in residual stress and rough morphology. The nc‐TiAlN/a‐Si3N4 nanocomposite structure was obtained in coatings. The highest hardness and Young's modulus reached 42.4 and 495 GPa at −120 V, respectively. However, at −60 V, the coatings with the highest H/E* and H3/E*2 ratios of 0.095 and 0.332 exhibited excellent adhesion with above HF1 level, the lowest coefficient of friction (COF) of 0.35 and specific wear rate of 2.1 × 10−7 mm3 N−1 m−1. Wear mechanism changed from the mixture of severe adhesive, oxidative and abrasive wear to mild oxidative wear to severe oxidative wear. TiAlSiN coatings with high hardness and H/E* and H3/E*2 ratios exhibited the decrease in COF and wear rate due to refined grains in uniform distribution, which well promoted oxide layers formed on sliding contact surface.
Association between polymorphisms in the promoter region of miR‐17‐92 cluster and systemic lupus erythematosus in a Chinese population
Authors: Wang Rong , Wang Chun‐Fang , Qin Hai‐Mei , Lu Yu‐Lan , Wei Gui‐Jiang , Huang Hua‐Tuo , Xiang Yang , Wang Jun‐Li , Lan Yan , Wei Ye‐Sheng 
Published: May 2018

AbstractThe aim of this study was to investigate the association of genetic polymorphisms in the promoter region of miR‐17‐92 with systemic lupus erythematosus (SLE). The gene polymorphism was analysed using SNaPshot in 312 SLE patients and 396 controls. Relative expression of miR‐17‐92 was measured by quantitative real‐time PCR. Association was found between rs9515692 and a decreased risk of SLE (CT vs CC: OR = 0.65, 95%CI, 0.46‐0.92, P = .014; CT+TT vs CC: OR = 0.64, 95%CI, 0.46‐0.90, P = .009; T vs C: OR = 0.69, 95%CI, 0.52‐0.92, P = .010, respectively). Haplotype analysis showed that C‐G‐G, C‐A‐A haplotypes were associated with an increased SLE risk (OR=4.46, 95%CI, 2.17‐9.17, P < 0.001; OR=2.33, 95%CI, 1.44‐3.76, P < 0.001, respectively). T allele and CT+TT genotypes in rs9515692 were associated with decreased risk of anti‐dsDNA in SLE (CT+TT vs CC: OR = 0.42, 95%CI = 0.24‐0.72, P = .002; T vs A: OR = 0.49, 95%CI = 0.31‐0.79, P = .003). Moreover, rs9515692 CT+TT genotypes had a higher level of miR‐17 as compared to CC genotype (P = .017). These findings suggest that the rs9515692 CT+TT genotypes were a protective factor for the susceptibility of SLE, probably by increasing the expression of miR‐17.
Reproducing vulnerabilities in agri‐food systems: Tracing the links between governance, financialization, and vulnerability in Europe post 2007–2008
Authors: Marsden Terry , Moragues Faus Ana , Sonnino Roberta 
Published: May 2018

AbstractInterconnected sets of vulnerabilities have emerged in the European food system since 2007–2008, raising concerns about food security in a region with arguably some of the most advanced and prosperous economies and environmental governance frameworks. Historically, this is suggesting the current “double jeopardy” problem in food system vulnerability—with systemic declines both in sustainability (the ability of the food system to ecologically renew itself) and food security (the ability of a population to access sufficient nutritional foods and feed itself). By focusing on both drivers and impacts of food system vulnerabilities in the European Union and United Kingdom, this paper explores a grounded and relational approach to financialization—recognized as a key expression of recent growing vulnerabilities. Through the prism of the current socio‐economic pressures facing food producers, and the emergence of potentially “stranded assets” in the agri‐food system more generally, the analysis seeks to show how the combined relationships between neo‐liberalized governance and the market volatilities encouraged by new rounds of financialization are creating “nested” vulnerabilities. As we conclude, a critical, grounded, and systemic understanding of food system vulnerabilities thus becomes a key feature and precursor for potentially developing more resilient agri‐food systems—both regionally and globally.
Clinicopathological significance of chemokine receptor (CCR1, CCR3, CCR4, CCR5, CCR7 and CXCR4) expression in head and neck squamous cell carcinomas
Authors: González‐Arriagada Wilfredo A. , Lozano‐Burgos Carlos , Zúñiga‐Moreta Rodrigo , González‐Díaz Paulina , Coletta Ricardo D. 
Published: May 2018

BackgroundHead and neck squamous cell carcinoma shows high prevalence of lymph node metastasis at diagnosis, and despite the advances in treatment, the overall 5‐year survival is still under 50%. Chemokine receptors have a role in the development and progression of cancer, but their effect in head and neck carcinoma remains poorly characterised. This study aimed to assess the prognostic value of CCR1, CCR3, CCR4, CCR5, CCR7 and CXCR4 in head and neck squamous cell carcinomas.MethodsImmunohistochemical expression of chemokine receptors was evaluated in a retrospective cohort of 76 cases of head and neck squamous cell carcinoma. Clinicopathological associations were analysed using the chi‐square test, survival curves were analysed according to the Kaplan‐Meier method, and the Cox proportional hazard model was applied for multivariate survival analysis.ResultsThe chemokine receptors were highly expressed in primary carcinomas, except for CCR1 and CCR3. Significant associations were detected, including the associations between CCR5 expression and lymph node metastasis (N stage, P = .03), advanced clinical stage (P = .003), poor differentiation of tumours (P = .05) and recurrence (P = .01). The high expression of CCR5 was also associated with shortened disease‐free survival (HR: 2.85, 95% CI: 1.09‐8.14, P = .05), but the association did not withstand the Cox multivariate survival analysis. At univariate analysis, high expression of CCR7 was associated with disease‐free survival and low levels of CXCR4 were significantly associated with both disease‐specific and disease‐free survival.ConclusionsThese findings show that chemokine receptors may have an important role in head and neck squamous cell carcinoma progression, regional lymph node metastasis and recurrence.
Characterization of Colletotrichum orchidophilum, the agent of black spot disease of vanilla
Authors: Charron Carine , Hubert Jacqueline , Minatchy Janice , Wilson Véronique , Chrysot Fabiola , Gerville Scholastie , Ioos Renaud , Jeandel Céline , Grisoni Michel 
Published: May 2018

AbstractBlack spot symptoms were reported on vanilla plants in Reunion Island in 2011. They have repeatedly reduced annual pod production by 10% to 30%. The disease is characterized by dark spots that appear in slight depressions on flowers, pods, leaves and stems. The spots then develop into broad clearly depressed necrotic plaques. Morphological and molecular analyses, as well as pathogenicity tests, identified the fungus Colletotrichum orchidophilum (Ascomycota) as the causal agent of the disease. Inoculation tests in controlled conditions confirmed that the two C. orchidophilum strains isolated from fruit lesions are pathogenic on the leaves and fruits of Vanilla planifolia (accessions CR0001 and CR0020). However, these strains induced symptoms only when the epidermis of leaves and fruits had been punctured by a needle. In the absence of injury, no symptom appeared. Colletotrichum arxii and Fusarium proliferatum (Ascomycota) are fungal species that are also frequently isolated from black spot lesions. However, they are not pathogenic to vanilla. This is the first report of C. orchidophilum in Reunion Island. It is also the first demonstration of C. orchidophilum's pathogenicity to an orchid. Simple preventive control measures were proposed to reduce the incidence of black spot disease in vanilla plots.
Melatonin induction and its role in high light stress tolerance in Arabidopsis thaliana
Authors: Lee Hyoung Yool , Back Kyoungwhan 
Published: May 2018

AbstractIn plants, melatonin is a potent bioactive molecule involved in the response against various biotic and abiotic stresses. However, little is known of its defensive role against high light (HL) stress. In this study, we found that melatonin was transiently induced in response to HL stress in Arabidopsis thaliana with a simultaneous increase in the expression of melatonin biosynthetic genes, including serotonin N‐acetyltransferase1 (SNAT1). Transient induction of melatonin was also observed in the flu mutant, a singlet oxygen (1O2)‐producing mutant, upon light exposure, suggestive of melatonin induction by chloroplastidic 1O2 against HL stress. An Arabidopsis snat1 mutant was devoid of melatonin induction upon HL stress, resulting in high susceptibility to HL stress. Exogenous melatonin treatment mitigated damage caused by HL stress in the snat1 mutant by reducing O2− production and increasing the expression of various ROS‐responsive genes. In analogy, an Arabidopsis SNAT1‐overexpressing line showed increased tolerance of HL stress concomitant with a reduction in malondialdehyde and ion leakage. A complementation line expressing an Arabidopsis SNAT1 genomic fragment in the snat1 mutant completely restored HL stress susceptibility in the snat1 mutant to levels comparable to that of wild‐type Col‐0 plants. The results of the analysis of several Arabidopsis genetic lines reveal for the first time at the genetic level that melatonin is involved in conferring HL stress tolerance in plants.