bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular forms and fragments of salivary MMP‐8 in relation to periodontitis
Authors: Gürsoy Ulvi K. , Könönen Eija , Tervahartiala Taina , Gürsoy Mervi , Pitkänen Jari , Torvi Paula , Suominen Anna Liisa , Pussinen Pirkko , Sorsa Timo 
Published: October 2018

AbstractAimTo investigate the molecular forms of salivary matrix metalloproteinase (MMP)‐8 in relation to periodontitis.Materials and MethodsMolecular forms, degree of activation and fragmentation of neutrophilic and mesenchymal‐type MMP‐8 isoforms were analysed from salivary samples of 81 subjects with generalized periodontitis, 63 subjects with localized periodontitis and 79 subjects without pocket teeth, by using western‐immunoblots with computer quantitation. In addition, human recombinant proMMP‐8 was in vitro activated by Treponema denticola chymotrypsin‐like protease (Td‐CTLP), sodium hypochlorite (NaOCl, 1 mM, oxidant) or amino phenyl mercuric acetate (APMA, 1 mM).ResultsIn saliva of periodontitis‐affected individuals, MMP‐8 is found in multiple forms, that is, complexes, active and pro‐forms of neutrophilic and mesenchymal‐type MMP‐8, and especially 20–27 kDa fragments. The quantity of these fragments was elevated in both localized and generalized forms of periodontitis. Moreover, the tested activators (Td‐CTLP, NaOCl and APMA) activated inactive proMMP‐8, resulting in fragments of 20–27 kDa, in vitro, and salivary concentrations of T. denticola correlated significantly with salivary levels of fragmented MMP‐8.ConclusionThe present results indicate that during the development and progression of periodontitis, MMP‐8 appears as activated and fragmented, and treponemal proteases most likely play role in this cascade.
Randomized controlled multicentre study comparing short dental implants (6 mm) versus longer dental implants (11–15 mm) in combination with sinus floor elevation procedures: 5‐Year data
Authors: Thoma Daniel S. , Haas Robert , Sporniak‐Tutak Katarzyna , Garcia Abel , Taylor Thomas D. , Hämmerle Christoph H. F. 
Published: October 2018

AbstractAimTo compare the implant survival rate between short dental implants and standard length implants placed in combination with bone grafting at 5 years of loading.MethodsThis multicentre study enrolled 101 patients (137 implants) with a posterior maxillary bone height of 5–7 mm. Patients randomly received either short implants (6 mm; GS) or long implants (11–15 mm) with sinus grafting (GG). Six months later, implants were loaded with single crowns and patients re‐examined at 1, 3 and 5 years of loading. Outcomes included: implant survival, marginal bone levels (MBLs), biological and technical parameters and patient‐reported outcome measures (OHIP‐49 = Oral Health Impact Profile). Statistical analysis was performed using a non‐parametric approach.ResultsAt 5 years, 90 patients (124 implants; GS: 60; GG: 64) were re‐examined (drop‐out rate 10%). Patient‐level implant survival rates were 98.5% (GS; 1 implant failure) and 100% (GG; p = 0.49). Mean MBLs were 0.54 mm ± 0.87 (GS) and 0.46 mm ± 1.00 (GG; p = 0.34). Biological and technical parameters were not significantly different (p > 0.05). Median overall OHIP‐49 scores improved significantly up to 5 years in both groups (GS: p = 0.03; GG: p = 0.00; intergroup comparison p = 0.11).ConclusionsBoth treatment modalities were suitable for implant therapy in the atrophied posterior maxilla revealing no differences in terms of survival rates, marginal bone levels (changes), patient‐reported outcomes and technical/biological complications.
Swarming and Aggregation in the Parasitic Diplomonad Flagellate Spironucleus vortens
Authors: Poynton Sarah L. , Ostrenga Lauren , Witwer Kenneth W. 
Published: October 2018

AbstractPathogenicity, evolutionary history, and unusual cell organization of diplomonads are well known, particularly for Giardia and Spironucleus; however, behavior of these aerotolerant anaerobes is largely unknown. Addressing this deficit, we studied behavior of the piscine diplomonad Spironucleus vortens (ATCC 50386) in in vitro culture. Spironucleus vortens trophozoites from Angelfish, Pterophyllum scalare, were maintained axenically in modified liver digest, yeast extract, and iron (LYI) medium, at 22 °C in the dark, and subcultured weekly. Cultures were monitored every 1–2 d, by removing an aliquot, and loading cells into a hemocytometer chamber, or onto a regular microscope slide. We observed three distinct swimming behaviors: (i) spontaneous formation of swarms, reaching 200 μm in diameter, persisting for up to several min in situ, (ii) directional movement of the swarm, via collective motility, and (iii) independent swimming of trophozoites to form a band (aggregation), presumably at the location of optimal environmental conditions. These behaviors have not previously been reported in Spironucleus. The observation that flagellate motility can change, from individual self‐propulsion to complex collective swarming motility, prompts us to advocate S. vortens as a new model for study of group behavioral dynamics, complementing emerging studies of collective swimming in flagellated bacteria.
Significance of hypovascular lesions on dynamic computed tomography and/or gadolinium ethoxybenzyl diethylenetriamine pentaacetic acid‐enhanced magnetic resonance imaging in patients with hepatocellular carcinoma
Authors: Gyoda Yu , Imamura Hiroshi , Ichida Hirofumi , Yoshimoto Jiro , Ishizaki Yoichi , Kuwatsuru Ryohei , Kawasaki Seiji 
Published: October 2018

AbstractBackground and AimThe natural course and clinical implications of hypovascular lesions on dynamic computed tomography and/or gadolinium ethoxybenzyl diethylenetriamine pentaacetic acid‐enhanced magnetic resonance imaging were investigated.MethodsWe followed the patients with hepatocellular carcinoma (HCC) who underwent hepatectomy between April 2009 and August 2012 to determine whether new classical HCCs developed from these unresected borderline lesions or emerged in different areas.ResultsOne hundred and eleven patients with HCC were identified to have undergone examinations using both imaging methods before hepatic resection. A total of 54 hypovascular lesions were detected. Gadolinium ethoxybenzyl‐enhanced magnetic resonance imaging detected 51 lesions, while dynamic computed tomography identified 21 lesions. Eleven lesions were resected at the time of the hepatectomy together with the main HCCs. Classical HCCs had developed from 52.5% of the 43 unresected lesions at 3 years after hepatic resection. Subsequently, we conducted a patient‐by‐patient analysis to compare the development of classical HCC from these hypovascular lesions and the emergence of de novo classical HCC in other areas. The 3‐year occurrence rate was 62.2% for the former group and 55.0% for the latter group (P = 0.83). Thus, although 52.2% of these hypovascular lesions had developed into classical HCCs at 3 years after the initial hepatectomy, de novo HCCs also occurred at other sites. Furthermore, new hypovascular lesions emerged after hepatectomy in 18–29% of patients irrespective of the presence or absence of hypovascular lesions at hepatectomy.ConclusionsIt remains uncertain whether these hypovascular lesions should be resected together with the main tumors at the time of hepatectomy.
Cellophane surface‐induced gene, VdCSIN1, regulates hyphopodium formation and pathogenesis via cAMP‐mediated signalling in Verticillium dahliae
Authors: Sun Lifan , Qin Jun , Rong Wei , Ni Hao , Guo Hui‐Shan , Zhang Jie 
Published: October 2018

SummaryThe soil‐borne vascular pathogen Verticillium dahliae infects many dicotyledonous plants to cause devastating wilt diseases. During colonization, V. dahliae spores develop hyphae surrounding the roots. Only a few hyphae that adhere tightly to the root surface form hyphopodia at the infection site, which further differentiate into penetration pegs to facilitate infection. The molecular mechanisms controlling hyphopodium formation in V. dahliae remain unclear. Here, we uncovered a cellophane surface‐induced gene (VdCSIN1) as a regulator of V. dahliae hyphopodium formation and pathogenesis. Deletion of VdCSIN1 compromises hyphopodium formation, hyphal development and pathogenesis. Exogenous application of cyclic adenosine monophosphate (cAMP) degradation inhibitor or disruption of the cAMP phosphodiesterase gene (VdPDEH) partially restores hyphopodium formation in the VdΔcsin1 mutant. Moreover, deletion of VdPDEH partially restores the pathogenesis of the VdΔcsin1 mutant. These findings indicate that VdCSIN1 regulates hyphopodium formation via cAMP‐mediated signalling to promote host colonization by V. dahliae.
Water fluxes mediated by vegetation: emerging isotopic insights at the soil and atmosphere interfaces
Authors: Dubbert Maren , Werner Christiane 
Published: October 2018

SummaryPlants mediate water fluxes within the soil–vegetation–atmosphere continuum. This water transfer in soils, through plants, into the atmosphere can be effectively traced by stable isotopologues of water. However, rapid dynamic processes have only recently gained attention, such as adaptations in root water uptake depths (within hours to days) or the imprint of transpirational fluxes on atmospheric moisture, particularly promoted by the development of real‐time in‐situ water vapour stable isotope observation techniques. We focus on open questions and emerging insights at the soil–plant and plant–atmosphere interfaces, as we believe that these are the controlling factors for ecosystem water cycling. At both interfaces, complex pictures of interacting ecophysiological and hydrological processes emerge: root water uptake dynamics depend on both spatiotemporal variations in water availability and species‐specific regulation of adaptive root conductivity within the rooting system by, for example, modulating soil–root conductivity in response to water and nutrient demands. Similarly, plant water transport and losses are a fine‐tuned interplay between species‐specific structural and functional strategies of water use and atmospheric processes. We propose that only by explicitly merging insights from distinct disciplines – for example, hydrology, plant physiology and atmospheric sciences – will we gain a holistic picture of the impact of vegetation on processes governing the soil–plant–atmosphere continuum.
The fixation tines of the Micra™ leadless pacemaker are atraumatic to the tricuspid valve
Authors: Mattson Alexander R. , Zhingre Sanchez Jorge D. , Iaizzo Paul A. 
Published: October 2018

AbstractBackgroundToday, there is no manufacturer‐supplied retrieval tool for the Micra™ pacemaker (Medtronic, Minneapolis, MN, USA); therefore, off‐the‐shelf catheters have been employed for retrievals. The proximal retrieval feature of the Micra™ can be snared and the device is then retracted from the myocardium, pulling the device through the tricuspid valve. This study characterizes the potential risks of Micra™ nitinol tine engagement with the tricuspid sub‐valvular apparatus.MethodsFresh human hearts nonviable for transplant (n = 10) were obtained from our regional organ procurement agency (LifeSource, Minneapolis, MN, USA). Micra™ fixation tines were affixed to a linear force transducer. Tines were then engaged in tricuspid chordae tendineae to conduct a constant velocity tensile test. Each test was run until tines disengaged from the chordae tendineae or until they released from the valve apparatus. Subsequently, biomechanical failure properties of the valve apparatus and isolated chordae tendineae were determined using a series of uniaxial tensile tests.ResultsThere were no chordal ruptures observed during our Micra™ tine extraction testing. Chordal failure required 15.0 times the force of extracting a single engaged tine, and 9.0 times the force of extracting two engaged tines. The uniaxial stresses required for isolated chordal failure averaged 17.4 N/mm2; failure strains exceeded 150% resting chordal length.ConclusionsThe forces required to rupture tricuspid chordae tendineae significantly exceeded the forces potentially imposed on the chordae during Micra™ device retrievals. We conclude that the fixation tines of the Micra™ device are unlikely to damage the tricuspid apparatus during either implant or retrieval.
Lectin AtGAL1 interacts with high‐mannose glycoform of the purple acid phosphatase AtPAP26 secreted by phosphate‐starved Arabidopsis
Authors: Ghahremani Mina , Park Joonho , Anderson Erin M. , Marty‐Howard Naomi J. , Mullen Robert T. , Plaxton William C. 
Published: October 2018

AbstractAmong 29 predicted Arabidopsis purple acid phosphatases (PAPs), AtPAP26 functions as the principle extracellular and intracellular PAP isozyme that is upregulated to recycle and scavenge Pi during Pi‐deprivation or leaf senescence. Our companion paper documented the copurification of a secreted, high‐mannose AtPAP26‐S2 glycoform with AtGAL1 (At1g78850), a Pi starvation‐inducible (PSI), and Galanthus nivalis agglutinin‐related (mannose‐binding) and apple domain lectin. This study tests the hypothesis that AtGAL1 binds AtPAP26‐S2 to modify its enzymatic properties. Far‐western immunodot blotting established that AtGAL1 readily associates with AtPAP26‐S2 but not the low mannose AtPAP26‐S1 glycoform nor other secreted PSI PAPs (i.e., AtPAP12 or AtPAP25). Analytical gel filtration indicated that 55‐kDa AtGAL1 and AtPAP26‐S2 polypeptides associate to form a 112‐kDa heterodimer. Microscopic imaging of transiently expressed, fluorescent protein‐tagged AtGAL1, and associated bimolecular fluorescence complementation assays demonstrated that (a) like AtPAP26, AtGAL1 also localizes to lytic vacuoles of Pi‐deprived Arabidopsis and (b) both proteins interact in vivo. AtGAL1 preincubation significantly enhanced the acid phosphatase activity and thermal stability of AtPAP26‐S2 but not AtPAP26‐S1. We hypothesize that AtGAL1 plays an important role during Pi deprivation through its interaction with mannose‐rich glycans of AtPAP26‐S2 and consequent positive impact on AtPAP26‐S2 activity and stability.
ABO‐incompatible liver transplantation for children under 2 years of age: A case report and a single‐center review
Authors: Narumoto Soichi , Sakamoto Seisuke , Sasaki Kengo , Hirata Yoshihiro , Fukuda Akinari , Uchiyama Toru , Irie Rie , Yoshioka Takako , Kasahara Mureo 
Published: October 2018

AbstractDesensitization with RTX has been broadly introduced in adult LT across the ABO blood type barrier. For pediatric LT, the prophylactic use of RTX has not been standardized, especially for children under 2 years of age. A 20‐month‐old girl with BA underwent living donor LT from her ABO‐I mother. On POD 6, she developed combined T cell‐mediated and AMRs. Steroid bolus injection was immediately introduced, followed by antibody‐depleting therapy with PE and IVIG. Based on a peripheral blood lymphocyte analysis by fluorescence‐activated cell sorting, ATG and RTX were introduced for refractory rejection. Although she recovered from the combined rejections, IHBCs were inevitable as a consequence. We recommend extending the desensitization protocol to cover children under 2 years of age in order to prevent life‐threatening complications.
PARADOXES AND STRUCTURAL RULES FROM A DIALOGICAL PERSPECTIVE
Authors: Dutilh Novaes Catarina , French Rohan 
Published: October 2018

AbstractIn recent years, substructural approaches to paradoxes have become quite popular. But whatever restrictions on structural rules we may want to enforce, it is highly desirable that such restrictions be accompanied by independent philosophical motivation, not directly related to paradoxes. Indeed, while these recent developments have shed new light on a number of issues pertaining to paradoxes, it seems that we now have even more open questions than before, in particular two very pressing ones: what (independent) motivations do we have (if any) for restrictions on structural rules, and what to make of the plurality of new logics emerging from these restrictions, i.e. how to ‘choose’ among the different options. In this paper, we address these two questions from the perspective of a dialogical conception of logic that we've been advocating in recent years. We will argue that dialogical interpretations of structural rules, that is, as rules determining specific properties of the dialogues in question, provide a conveniently neutral framework to adjudicate between the different substructural proposals that have been made in the literature on paradoxes.
Responses of the weed Bidens pilosa L. to exogenous application of the steroidal saponin protodioscin and plant growth regulators 24‐epibrassinolide, indol‐3‐acetic acid and abscisic acid
Authors: Mito M. S. , Silva A. A. , Kagami F. L. , Almeida J. D. , Mantovanelli G. C. , Barbosa M. C. , Kern‐Cardoso K. A. , Ishii‐Iwamoto E. L. 
Published: October 2018

Abstract

The exogenous application of plant hormones and their analogues has been exploited to improve crop performance in the field. Protodioscin is a saponin whose steroidal moiety has some similarities to plant steroidal hormones, brassinosteroids. To test the possibility that protodioscin acts as an agonist or antagonist of brassinosteroids or other plant growth regulators, we compared responses of the weed species Bidens pilosa L. to treatment with protodioscin, brassinosteroids, auxins (IAA) and abscisic acid (ABA).

Seeds were germinated and grown in agar containing protodioscin, dioscin, brassinolides, IAA and ABA. Root apex respiratory activity was measured with an oxygen electrode. Malondialdehyde (MDA) and antioxidant enzymes activities were assessed.

Protodioscin at 48–240 μm inhibited growth of B. pilosa seedlings. The steroidal hormone 24‐epibrassinolide (0.1–5 μm) also inhibited growth of primary roots, but brassicasterol was inactive. IAA at higher concentrations (0.5–10.0 μm) strongly inhibited primary root length and fresh weight of stems. ABA inhibited all parameters of seedling growth and also seed germination. Respiratory activity of primary roots (KCN‐sensitive and KCN‐insensitive) was activated by protodioscin. IAA and ABA reduced KCN‐insensitive respiration. The content of MDA in primary roots increased only after protodioscin treatment. All assayed compounds increased APx and POD activity, with 24‐epibrassinolide being most active. The activity of CAT was stimulated by protodioscin and 24‐epibrassinolide.

The results revealed that protodioscin was toxic to B. pilosa through a mechanism not related to plant growth regulator signalling. Protodioscin caused a disturbance in mitochondrial respiratory activity, which could be related to overproduction of ROS and consequent cell membrane damage.
Redeposition of volcaniclastic sediments by a tsunami 4600 years ago at Kushima City, south‐eastern Kyushu, Japan
Authors: Yamada Masaki , Fujino Shigehiro , Chiba Takashi , Goto Kazuhisa , Goff James 
Published: October 2018

AbstractThe Hyuga‐nada Sea, south‐eastern Kyushu, Japan, is located between a strong (Nankai Trough) and a weak interplate coupling zone (Ryukyu Trench). Over the past 400 years this area has only experienced Magnitude 7·5 earthquakes or smaller and associated small‐scale tsunamis. However, this short historical record most likely does not include the full range of high magnitude, low frequency giant earthquakes that might have occurred in the region. Thus, it is still unclear whether giant earthquakes and their associated tsunamis have occurred in this region. This paper reports on a prehistoric tsunami deposit discovered in a coastal lowland in south‐eastern Kyushu facing the Hyuga‐nada Sea. There is a reddish‐brown pumiceous layer preserved in a non‐marine, organic‐rich mud sequence obtained from onshore sediment cores. This layer is recognized as the ca 4600 year old Kirishima‐Miike tephra (that is now placed around 4500 years ago) sourced from Mount Kirishima, southern Kyushu. Another whitish pumiceous layer is evident below the Kirishima‐Miike tephra in almost all of the sediment cores. A relatively high percentage of marine and brackish diatoms is recorded within this lower pumiceous layer (but not in the surrounding muds or in the overlying Kirishima‐Miike tephra), indicating a marine or beach sediment source. Plant material obtained from organic‐rich mud immediately below the event layer was dated to ca 4430 to 4710 cal yr bp, providing a limiting‐maximum age for this marine incursion event. The presence of marine diatoms below the event layer is probably explained by pre‐seismic subsidence. An absence of the resting spore of the planktonic brackish diatom Cheatoceros and the appearance of the freshwater diatom Eunotia serra immediately above the event layer probably represents a marked change to a relatively low‐salinity environment. Assuming that there were no significant local geomorphological changes, such as drainage obstruction caused by formation of a new barrier spit, it is considered that co‐seismic or immediate post‐seismic uplift are the most likely explanations for this notable environmental change. Based on the crustal movements noted before and after the marine incursion, this event is interpreted here as an earthquake‐generated tsunami. Moreover, because of these notable seismic crustal movements the tsunamigenic earthquake probably occurred immediately offshore of the study site.
The roles of SOCS3 and STAT3 in bacterial infection and inflammatory diseases
Authors: Gao Yu , Zhao Honglei , Wang Peng , Wang Jun , Zou Lili 
Published: October 2018

AbstractThe suppressor of cytokine signalling‐3 (SOCS3) is well known as a feedback inhibitor of the Janus kinases‐signal transducer and activator of transcription 3 (JAK/STAT3) signalling pathway, which mediates the signal transduction of many cytokines, growth factors and hormones during many cellular processes. The critical role of SOCS3 is manifested by its binding to both the JAK kinase and the cytokine receptor, which can result in the inhibition of STAT3 phosphorylation. STAT3 triggers variety of genes expression in response to cytokine (IL‐6 family, IL‐10) and growth factor stimulation and thus plays a critical role in many cellular biological processes involved in anti/pro‐inflammatory responses, cell growth and cell death. SOCS3 might act directly either by hampering JAK activation or by mediating the ubiquitination and subsequent proteasome degradation of the cytokine/growth factor/hormone receptor. Generally, SOCS3 is a negative regulator for cytokine or hormone signalling. But in some cases, SOCS3 regulates the inflammatory responses positively through inhibiting STAT3. An increasing number of reports showed abnormal expression levels of SOCS3/STAT3 in different myeloid and lymphoid cells as well as in various non‐hematopoietic cells, which suggested its involvement in various infection and inflammatory diseases. In this review, we described the role of SOCS3 and STAT3 expression in different cell populations in regulating the outcomes of infection and inflammatory diseases.