bluuebunny/crossref_metadata_2025_split huggingface dataset:
Green Tea Yoghurt with Encapsulated Lacticaseibacillus paracasei E1 Improves Hepatocyte Damage in High-Fat High-Fructose Diet Mice by Reducing MDA and Increasing SOD
Authors: Fadlilah Dawama Nur , Izati Rahmi , Al Faizah Belinda Nabiila , Kavitarna Septhyanti Aprilia , Ardiansyah Esha , Sa'adah Nur Alfi Maghfirotus , Atho'illah Mochammad Fitri , Arifah Siti Nur , Soewondo Aris , Jatmiko Yoga Dwi , Wardhani Shinta Oktya , Barlianto Wisnu , Rifa'i Muhaimin 
Published: November 2024

Background: Obesity is a global epidemic caused by excessive body fat, which is increasing free fatty acids in the liver, causing oxidative stress and liver cell damage. Green tea yogurt (GTY) with encapsulated Lacticaseibacillus paracasei E1 (GTY-LpE1) might have a beneficial effect in reducing liver cell damage. This study was conducted determine GTY-LpE1 effect on superoxide dismutase (SOD) expression, malondialdehyde (MDA) expression and liver histopathology in high-fat high-fructose diet (HFFD) mice. Material and Methods: A completely randomized design (CRD) with 7 groups, including normal diet (ND) group, HFFD group, 1.3 mg/kg BW simvastatin (SIM)-administered HFFD group, 5 g/kg BW probiotic yoghurt (PY)-administered HFFD (PY), 2.5 g/kg BW GTY-administered HFFD (2.5 GTY), 5 g/kg BW GTY-administered HFFD (5 GTY), and 10 g/kg BW GTY-administered HFFD (10 GTY). The diet was given for 16 weeks, followed by oral administration of sim/yoghurt during the last 4 weeks. Mice were sacrificed and the liver was collected. SOD and MDA expression were analyzed by flow cytometry. Histopathology analysis was done by evaluating hematoxylin-eosin (HE) staining of the liver.Result: The percentage of necrotic cells were 34.55, 34.31, and 21.95%, when treated with 2.5, 5, and 10 g/kg BW with GTY-administered HFFD, respectively, these were lower than the ones in the HFFD group (69.49%). The percentage of MDA expression were 15.55, 18.69, and 22.42%, respectively, these were lower than the ones in the HFFD group as well. The percentage of SOD expression were 9.49, 7.85, and 11.11%, respectively, these were higher than the ones in the HFFD group (3.44%). Conclusion: GTY-LpE1 could decrease the number of necrotic cells in the HFFD mice livers and improve the hepatocyte damage by reducing MDA expression and enhancing SOD expression. GTY-LpE1 can be used as an alternative food to control obesity.Keywords: alginate, chitosan, encapsulation, green tea, probiotic
Fatores geradores de estresse para pessoa com estomia intestinal - impactos na Saúde Mental e autocuidado
Authors: Pires Tarsila Reis Pinto , Siqueira Milena Rangel , Pedrosa Pietro Henrique Benevides , Ribeiro Wanderson Alves , Fassarella Bruna Porath Azevedo , Neves Keila do Carmo 
Published: November 2024

Introdução: Estresse tem sido visto como um dos males da vida moderna e frequentemente é um termo utilizado para mencionar sintomas físicos e psicológicos provocados por pressões e adaptações do dia a dia que podem ser exacerbados ao longo do processo saúde-doença de uma pessoa com estomia intestinal. O paciente estomizado se vê numa situação complexa e difícil, onde pode ocorrer o isolamento psicológico e social, interferindo assim nos seus relacionamentos e trabalho trazendo consigo sentimentos negativos, ansiedade, medo e as dúvidas. Objetivo: Analisar os fatores de estresse que acomete a pessoa com estomia intestinal. Métodos: Trata-se de um estudo exploratório descritivo, tendo como fonte de informação a pesquisa de campo e abordagem mista (quanti + quali) Se tratando de pesquisas que envolvem o ser humano, o estudo foi submetido no Comitê de Ética em Pesquisa, com parecer de aprovação de número 5.795.520 no dia 06/12/2022. Resultados: Foram coletados e analisados dados de 25 participantes, no qual demonstrou a prevalência de estomia em mulheres, a maior parte com diagnóstico de câncer colorretal, o estudo mostrou que 88% dos entrevistados possuem ensino medio completo ou superior o que corrobora para o melhor entendimento e boas práticas no autocuidado. Conclusão: Os resultados obtidos revelaram fragilidade emocional, perca da autoestima e o impacto na saúde mental. Além disso, foi observado que a fé, o apoio familiar e a busca por atividades prazerosas foram estratégias de enfrentamento utilizadas para lidar com o estresse e promover o autocuidado.
Palavras-Chave: Enfermagem; Estresse Emocional; Estomia.
Nível de informação sobre Saúde Bucal de Educadores de comunidades em situação de vulnerabilidade
Authors: Nunes Luana Batista , Silva Amanda Marques , Santos Amanda Fonseca dos , Lucietto Deison Alencar , Senna Marcos Antônio Albuquerque de , Teixeira Michelle Cecille Bandeira 
Published: November 2024

A educação em saúde é relevante estratégia para a promoção da saúde e para a prevenção de doenças bucais. Neste processo, professores da educação básica atuam como importantes aliados para divulgar e estimular hábitos saudáveis na rotina de crianças e adolescentes. Este estudo teve por objetivo descrever o nível de informação sobre saúde bucal de educadores de comunidades em situação de vulnerabilidade na cidade do Rio de Janeiro/RJ. Tratou-se de estudo descritivo realizado por meio de questionários com 38 educadores de uma Organização Não Governamental que atua nas comunidades do Pavão-Pavãozinho e Cantagalo. Verificou-se que a maioria dos profissionais possuía informações sobre saúde bucal. Contudo, foram observadas lacunas em conhecimentos sobre aleitamento materno, aleitamento e saúde bucal, dieta e saúde bucal, biofilme dental e mecanismo de ação dos açúcares na cárie dentária. O estudo apontou necessidade de aprimoramentos no nível de informação dos educadores sobre saúde bucal. A realização de ações de educação continuada e de educação permanente poderá contribuir na disseminação de saberes e práticas sobre saúde bucal na comunidade escolar.
Palavras-Chave: Saúde Bucal; Educação em Saúde; Vulnerabilidade Social; Acesso à informação; Docentes; Estudantes.
Benefícios do treinamento resistido no tratamento de pacientes com fibromialgia: uma revisão de literatura
Authors: Souza Rodrigo Goulart de , Trajano Larissa Alexsandra da Silva Neto , Fontes Ibérico  Alves 
Published: November 2024

A fibromialgia (FM) é uma síndrome crônica caracterizada por dor generalizada nos músculos e articulações, acompanhada de fadiga e outros sintomas. Essa condição afeta uma parcela significativa da população, principalmente mulheres. Embora não haja cura para a FM, existem abordagens terapêuticas, incluindo tratamentos medicamentosos e comportamentais, que visam melhorar a qualidade de vida dos pacientes. O objetivo desta revisão foi avaliar os benefícios do treinamento resistido no tratamento de indivíduos com fibromialgia. Uma pesquisa foi conduzida nas plataformas PubMed e Biblioteca Virtual em Saúde (BVS) com os descritores (Fibromyalgia) AND (exercise) AND (treatment) AND (Resistance Training). Foram incluídos 150 artigos que, após filtragem por data de publicação menor de 10 anos e tipo de estudo. Foram excluídos artigos duplicados e que o tema não era compatível com a pesquisa resultando em 17 artigos científicos. A análise desses estudos revelou que o treinamento resistido proporciona diversos benefícios para pacientes com fibromialgia, sendo a redução da dor crônica o benefício primordial. Apesar de a fisiopatologia da fibromialgia não ser completamente compreendida, existem evidências dos benefícios do treinamento resistido no tratamento desses pacientes, apresentando-se como uma alternativa aos exercícios aeróbicos ou como uma abordagem complementar. Em conclusão, é crucial para os profissionais de saúde reconhecerem que o exercício resistido desempenha um papel positivo no tratamento da fibromialgia, podendo ser indicado como uma opção independente ou em associação com exercícios aeróbicos de baixo impacto promovendo a melhora na qualidade de vida das mulheres que enfrentam a fibromialgia.
Palavras-chave: Fibromialgia; Tratamento; Treinamento Resistido.
TLR2 Activation as a Marker of Severe COVID-19 and a Potential Therapeutic Target
Authors: Mao Xianxian , Wu Yijia , Zhang Xinyi , Zhou Tian , Huang Houda , Fu Mingui , Qian Yisong 
Published: November 2024

Abstract:
SARS-CoV-2-induced COVID-19 has been a serious public health problem,
resulting in millions of lives lost over the previous three years. Although the direct infection
caused by virus invasion is important for the pathobiology of COVID-19, the hyperinflammatory
response and tissue injury are major contributors in critically ill patients.
As a host sensor, toll-like receptor 2 (TLR2) recognizes multiple pathogen-associated
molecular patterns (PAMPs) or damage-associated molecular patterns (DAMPs), producing
various inflammatory cytokines and inflammatory cell death signals, which are central
to the inflammatory pathology observed in COVID-19. The objectives of this narrative
review are to summarize the role of TLR2 activation during SARS-CoV-2 infection
and emphasize the importance of SARS-CoV-2 viral proteins in TLR2 activation. Additionally,
we presented some compounds related to TLR2 regulation clinically or experimentally,
which may provide new insights into targets for pharmaceutical discovery and
development.
Exosomal Delivery of miR-155 Inhibitor can Suppress Migration, Invasion, and Angiogenesis Via PTEN and DUSP14 in Triple-negative Breast Cancer
Authors: Razaviyan Javad , Sirati-Sabet Majid , Hadavi Razie , Karima Saeed , Rajabi Bazl Masoumeh , Mohammadi-Yeganeh Samira 
Published: November 2024

Introduction:
Triple-Negative Breast Cancer (TNBC) is the most common
type of breast cancer (BC). In order to develop effective treatments for TNBC, it is vital
to identify potential therapeutic targets. Angiogenesis stimulates tumor growth and metastasis
in TNBC, and miR-155 plays a crucial role in this process. The exosome is a
nano-sized vesicle that carries many cargoes, including miRNAs. The present study investigated
the effect of exosomal delivery of miR-155 antagomir on tumor migration, invasion,
and angiogenesis in TNBC.

Materials and methods:
From MDA-MB-231 cells, exosomes were extracted, characterized,
and loaded with miR-155 antagomir using electroporation. The expression of
miR-155 and its target genes, including PTEN and DUSP14, was analyzed using RTqPCR.
The wound-healing and transwell assays were used to measure cell migration and
invasion. Furthermore, angiogenesis was evaluated by tube formation and chorioallantoic
membrane (CAM) assays.

Results:
The results indicated that exosomal delivery of miR-155 antagomir to HUVEC
cells significantly suppressed miR-155 expression while upregulating PTEN and DUSP14.
The tube formation properties of HUVEC cells were also significantly reduced following
treatment with exosomes containing miR-155 antagomirs, and these results were
confirmed using CAM assay. The migration and invasion of MDA-MB-231 cells were
significantly reduced after treatment with miR-155 antagomir-loaded exosomes.

Conclusion:
It was found that miR-155 antagomir delivery using exosomes can inhibit
migration, invasion, and angiogenesis viaPTEN and DUSP14 in TNBC.
Galangin Regulates Astrocyte Phenotypes to Ameliorate Cerebral
Ischemia-reperfusion Injury by Inhibiting the RhoA/ROCK/LIMK Pathway
Authors: Liu Nannuan , Xu Yue , Liu Yao , Chen Tao , Hu Wenli 
Published: November 2024

Purpose:
This study aimed to explore whether Galangin (Gal) could improve cerebral Ischemia-
reperfusion (I/R) injury by regulating astrocytes, and clarify its potential molecular mechanism.

Methods:
An I/R injury model of rats was established using the Middle Cerebral Artery Occlusion/Reperfusion
(MCAO/R) method, followed by the administration of Gal (25, 50, 100 mg/kg) via gavage for 14 consecutive
days. Besides, astrocytes were isolated from the rats to construct an Oxygen-Glucose Deprivation/Re-oxygenation
(OGD/R) cell model, with treatments of Gal or the Ras homolog gene family member A
(RhoA)/Rho-associated Coiled-coil containing protein Kinase (ROCK) inhibitor Y-27632. Subsequently, the
severity of nerve injury was assessed using the modified Neurological Severity Score (mNSS) test; behavioral
disorders in I/R rats were observed through the open field and ladder-climbing tests. Pathological damages and
neuron survival in the peri-infarct zone were examined by hematoxylin and eosin staining and NeuN staining,
respectively. Additionally, immunofluorescence staining was employed to determine astrocyte polarization
and TUNEL staining was carried out to measure the level of cell apoptosis; also, western blot was performed
to detect the expression of proteins related to the RhoA/ROCK/LIM domain Kinase (LIMK) pathway.

Results:
Gal significantly ameliorated the neurological and motor dysfunctions caused by I/R in rats, reduced
pathological damage in the peri-infarct zone, and promoted neuronal survival. Additionally, Gal increased the
number of A2 astrocytes, while it decreased the number of A1 astrocytes. In vitro experiments revealed that
the effect of Gal was consistent with that of Y-27632. Additionally, Gal significantly enhanced the survival of
OGD/R cells, increased the number of A2 astrocytes, and inhibited the expression of proteins associated with
the RhoA/ROCK pathway.

Conclusion:
Gal could reduce the level of apoptosis, promote the polarization of A2 astrocytes, and improve
cerebral I/R injury, and its mechanism may be related to the inhibition of the RhoA/ROCK pathway.
Influence of Nanomedicine as a Smart Weapon on Multidrug Resistance in Cancer Therapy
Authors: Paikray Safal Kumar , Sahoo Liza , Tripathy Nigam Sekhar , Dilnawaz Fahima 
Published: November 2024

Abstract:
Cancer is the leading cause of death worldwide. The effectiveness of chemotherapy in
cancer patients is still significantly hampered by Multidrug Resistance (MDR). Tumors exploit
the MDR pathways to invade the host and limit the efficacy of chemotherapeutic drugs that are
delivered as single drugs or combinations. Further, overexpression of ATP-binding Cassette
transporter (ABC transporter) proteins augments the efflux of chemotherapeutic drugs and lowers
their intracellular accumulation. Recent progress in the development of nanotechnology and
nanocarrier-based drug delivery systems has shown a better perspective with respect to the improvement
of cancer chemotherapy. Nanoparticles/nanomaterials are designed to target the immune
system and tumor microenvironment of cancer cells for a variety of cancer treatments in
order to improve bioavailability and reduce toxicity. This review elucidates the successful use of
nanomaterials for cancer therapy and addressing the MDR and throws some light on the present
obstacles impeding their translation to clinical use.
Cetuximab scFv-Modified 5-FU Loaded Chitosan Nanoparticles: "A Novel Therapeutic Platform."
Authors: Jalalvand Masumeh , Esmaeili Fariba , Falahzadeh Khadijeh , Mazloumi Mohammadali , Shahsavari Gholamreza , Bayat Elham , Zandsalimi Farshid , Talebkhan Yeganeh , Nematollahi Leila , Negahdari Babak 
Published: November 2024

Background:
Colorectal cancer [CRC] is among the most fatal types of cancer. An
active targeting delivery system that specifically interacts with CRC cells could improve the
therapy's outcomes. Herein, Cetuximab single-chain fragment variable antibody [scFv] fragments
were conjugated to the surface of 5-FU encapsulated chitosan nanoparticles [CS NPs] to
develop an effective therapeutic platform [scFv-CS/5-FU NPs].

Method:
CS/5-FU NPs were synthesized using a special fluidic system. Encapsulation efficiency
[EE], loading capacity [LC], and the drug release profile of the particles were determined. scFv
fragments were produced recombinantly and tailored on the surface of CS/5-FU NPs. The physicochemical
features of scFv-CS/5-FU NPs were also characterized. MTT and flow cytometry
assay investigated the toxicity effect of scFv-CS/5-FU NPs on the HCT116 cell line.

Results:
CS/5-FU NPs had a homogenous spherical shape. They possessed sustainable drugrelease
behavior. The produced scFv-CS/5-FU NPs were also spherical. scFv-CS/5-FU NPs significantly
decreased the viability of cancerous cells in a dose-dependent manner and induced
apoptosis in 97.97% of targeted cells.

Conclusion:
scFv-CS/5-FU NPs showed remarkable anti-CRC activity. This novel targeting
delivery system reduced the effective dose of 5-FU which is of vital importance to decrease the
devastating side effects of chemotherapy.
Facial Hyperpigmentation Following Adalimumab
Authors: Yasmine Salem Mahjoubi , Imen Aouinti , Israa Dahmani , Fatma Zgolli , Ons Charfi , Sihem El Aidli 
Published: November 2024

Background:
Tumor necrosis factor alpha (TNF-α) inhibitors, such as adalimumab,
have significantly advanced the treatment of inflammatory diseases. However, these therapies are
associated with various cutaneous adverse reactions.

Case Presentation:
We describe two rare instances of isolated facial hyperpigmentation induced
by adalimumab. Both patients presented with asymptomatic, isolated brown macules on the
cheeks following adalimumab treatment for ankylosing spondylitis. The hyperpigmentation appeared
shortly after starting the medication in both cases. In one case, the hyperpigmentation persisted
despite stopping the medication, while in the second case, it completely resolved within one
month after discontinuation. However, in the second patient, the hyperpigmentation recurred after
switching to certolizumab, another TNF-α inhibitor. No skin biopsies were performed, and both
patients were otherwise healthy, with normal laboratory evaluations.

Conclusion:
Hyperpigmentation is an uncommon adverse reaction of this class of drugs, with only
a few reported cases in the literature. The recurrence of hyperpigmentation after switching to
another TNF-α agent, certolizumab, further suggests that this reaction may be a class effect, adding
new insights into the spectrum of cutaneous side effects associated with TNF-α inhibitors.
Clinicians should consider this potential side effect in patients presenting with hyperpigmentation,
and sun protection should be recommended as a preventive measure.
Acute Myeloid Leukemia Presenting as Extensive Arterial and Venous Thrombosis: A Case Report
Authors: Kachhwaha Arjun , Shah Bibhant , Ronanki Kavya , Dalton Prisla Maria , Nath Uttam Kumar 
Published: November 2024

Background:
Thromboembolism with solid malignancies is a commonly associated feature,
which is less common in hematological malignancies. Disseminated intravascular coagulation
(DIC) causing thrombotic events is characteristically associated with certain hematological
malignancies (e.g., acute promyelocytic leukemia (APL). Acute myeloid leukemia (AML) presenting
as extensive thromboembolism is not a common clinical presentation. Anticoagulation in these
subsets of patients remains a major challenge since patients often have thrombocytopenia and
bleeding manifestations, requiring close monitoring.

Case Presentation:
A 54-year-old male with a known case of ischemic heart disease on regular anti-
platelet therapy presented with acute onset progressive shortness of breath with mild anemia.
On further evaluation, the patient was diagnosed with bilateral pulmonary artery and venous
thrombosis along with left complete renal and partial inferior vena cava (IVC) thrombosis. The patient
was started safely on anticoagulant therapy with normal platelet counts. Later, peripheral
smear and immunophenotyping by flow cytometry revealed the diagnosis of acute myeloid
leukemia, and the patient started its treatment.

Conclusion:
Extensive arterial and venous thrombosis at presentation of acute myeloid leukemia
is an uncommon finding and needs anticoagulation therapy along with the treatment of the underlying
disease. Close monitoring of bleeding and maintaining an adequate platelet count is required,
especially in hematological malignancies.
Impact of Anaemia on Cognitive Impairment and Depression Levels Among Young Adults: A Cross-sectional Study Highlighting Gender Disparities
Authors: Chandrasekaran Vinitha , Varghese Neena Elsa , K Krishnaveni , R Kameswaran 
Published: November 2024

Background:
Anaemia is a significant health epidemic affecting all
age groups, especially adults. This study aimed to identify the impact of cognitive
impairment and depression levels according to potential risk factors among young
anaemic adults.

Materials and Methods:
A cross-sectional study was conducted among 546
adults aged 17-25 years. Haemoglobin levels were measured using Sahli's
method. Socio-demographic and risk factor data were collected via a
questionnaire. Depression and cognitive impairment levels were assessed using
the Patient Depression Questionnaire-9 (PDQ-9) and the Cognitive Assessment
Questionnaire (CAQ), respectively. ANOVA and Spearman's correlation were
used to explore the relationship between anaemia, cognitive impairment, and
depression levels. The mean age of participants was 19.41 years (SD = 1.47).
Gender distribution included 204 males (mean age = 19.2 years, SD = 1.4) and
342 females (mean age = 19.5 years, SD = 1.5).

Results:
The overall prevalence of anaemia was 73.2%, with 29.4% males and
43.7% females. Most anaemic patients (52.3%) had a mild degree of anaemia.
Among anaemic patients, 48.25% experienced forgetfulness, 44.25% had
distractibility, 25.7% had false triggering, and 16.2% reported no cognitive
issues. About 37.2% of anaemic patients fell under minimal depression.

Conclusion:
The study concludes that there is a significant impact of anaemia on
cognitive impairment and depression levels among young adults. Therefore,
raising awareness about anaemia in young adults is crucial to prevent
complications later in adulthood and during pregnancy.
Prooxidant Activity of Gallic Acid by Promote Reactive Oxygen Species, Apoptosis and Autophagy in HepG2 Cells In Vitro
Authors: Sabahi Zahra , Aghashiri Zahra , Emami Mahsa Zare , Shafaghat Marzieh , Iranpak Forough , Arabsolghar Rita , Alimohammadi Mahshid , Rashedinia Marzieh 
Published: November 2024

Introduction:
Gallic acid (GA), a natural phenolic acid, has been reported as an antitumor
agent in various cancer cells. Although some mechanisms, such as apoptosis, are well
known, the details of other mechanisms, such as their pro-oxidant and autophagy activity, are still
considerable.

Methods:
The pro-oxidative activity and anti-proliferative activity of GA on HEK 293 and HepG2
cells were measured in the absence and presence of exogenous Cu (II) and Fe (II). Furthermore,
colony forming, ROS generation, apoptosis induction, autophagy and mitochondrial membrane potential
(MMP) were examined.

Results:
HepG2 cells treated with GA + Cu (II) significantly reduced cell viability (p <0.001). GA
+Cu (II) induced morphological changes in HepG2 cells and stimulated apoptotic cell death. Moreover,
GA +Cu (II) triggered the mitochondrial-dependent apoptotic pathway by increasing intracellular
ROS levels and disrupting MMP. Furthermore, GA+ Cu (II) significantly reduced the Plating Efficiency
and Surviving Fraction while increasing autophagic vacuoles in the HepG2 cells.

Conclusion:
According to our results, GA played a pro-oxidant role in the presence of Cu (II), triggered
apoptosis by increased ROS and disruption of MMP. This combination also induced autophagy
in HepG2. These effects hold promise for future anticancer research.