bluuebunny/crossref_metadata_2025_split huggingface dataset:
RETRACTED ARTICLE: Circular RNA hsa_circ_0055538 regulates the malignant biological behavior of oral squamous cell carcinoma through the p53/Bcl-2/caspase signaling pathway
Authors: Su Wen , Sun Shuai , Wang Feng , Shen Yuehong , Yang Hongyu 
Published: March 2019

Abstract
Background
Oral squamous cell carcinoma (OSCC) is a common oral and maxillofacial malignant tumor with high rates of metastasis and mortality. Circular RNAs (circRNAs), a type of non-coding RNA, are involved in the development of a variety of tumors. The roles of circRNAs in OSCC are unclear; in this study, the correlation between the circRNA hsa_circ_0055538, previously identified by high-throughput sequencing, and the biological behavior of OSCC was evaluated.

Methods
circRNA expression was evaluated using patient tissue samples and various OSCC cell lines. The effects of overexpression and knockdown were evaluated by lentiviral infection and siRNA transfection of the SCC9 and CAL27 cell lines. Migration, invasion, apoptosis, and the expression of proteins in the p53 signaling pathway were evaluated. Infected cells were injected into nude mice to evaluate tumorigenesis.

Results
Low hsa_circ_0055538 expression levels were verified in tumor tissues and OSCC cell lines. Clinical data analysis showed that the expression level is related to the degree of tumor differentiation. Lentiviral infection and siRNA transfection of SCC9 and CAL27 cell lines revealed that changes in circRNA expression significantly affected the malignant biological behavior of OSCC cells. Importantly, nude mouse experiments showed that high expression of hsa_circ_0055538 inhibited tumor growth. Finally, hsa_circ_0055538 may affect the development of OSCC via the p53/Bcl-2/caspase signaling pathway.

Conclusions
Our results indicated that hsa_circ_0055538 is involved in OSCC via the p53 signaling pathway and may be a diagnostic and/or prognostic marker as well as a therapeutic target.
Infrared bundle adjusting and clustering method for head-mounted display and Leap Motion calibration
Authors: Park San , Cho Seoungjae , Park Jisun , Huang Kaisi , Sung Yunsick , Cho Kyungeun 
Published: March 2019

Abstract
Leap Motion has become widely used due to its ability to recognize intuitive hand gestures or accurate finger positions. Attaching a Leap Motion to a virtual reality head-mounted display (VR HMD) is highly interoperable with virtual objects in virtual reality. However, it is difficult for a virtual reality application to identify the accurate position where the Leap Motion is attached to the HMD. This causes errors in the positions of the actual user’s hands and the virtual hands, which makes the interaction in virtual reality difficult. In this paper, a method that calibrates an output area in VR HMD and a sensing area in Leap Motion is proposed. The difference in the origin coordinate between VR HMD and Leap Motion is derived using the proposed method. The position of the Leap Motion attached to the HMD was determined through an experiment using the proposed calibration technique, and the error was approximately 0.757 cm. Accordingly, it enables more intuitive interactions in virtual reality applications.
Earthquake-induced prompt gravity signals identified in dense array data in Japan
Authors: Kimura Masaya , Kame Nobuki , Watada Shingo , Ohtani Makiko , Araya Akito , Imanishi Yuichi , Ando Masaki , Kunugi Takashi 
Published: March 2019

AbstractEarthquake ruptures cause mass redistribution, which is expected to induce transient gravity perturbations simultaneously at all distances from the source before the arrival of P-waves. A recent research paper reported the detection of such prompt gravity signals from the 2011 Tohoku-Oki earthquake by comparing observed acceleration waveforms and model simulations. The 11 observed waveforms presented in that paper recorded in East Asia shared a similar trend above the background seismic noise and were in good agreement with the simulations. However, the signal detection was less quantitative because the significance of the observed signals was not discussed and the waveforms at other stations in the region were not shown. In this study, similar trends were not observed in most of the data recorded near the stations used in the aforementioned study, suggesting that the reported signals were only local noises. We thus took a different approach to identify the prompt signals. We optimized the multi-channel data recorded by superconducting gravimeters, broadband seismometers, and tiltmeters. Though no signal was identified in the single-trace records, the stacked trace of the broadband seismometer array data in Japan showed a clear signal above the reduced noise level. The signal amplitude was 0.25 nm/s2 for an average distance of 987 km from the event hypocenter. This detection was confirmed with a statistical significance of $$7\sigma$$

7
σ

, where $$\sigma$$
σ
is the standard deviation of the amplitude of the background noise. This result provided the first constraint on the amplitude of the observed prompt signals and may serve as a reference in the detection of prompt signals in future earthquakes.
Personal resilience in psychiatrists: systematic review
Authors: Howard Ranjita , Kirkley Catherine , Baylis Nicola 
Published: March 2019

Aims and methodThe concept of personal resilience is relevant to physician well-being, recruitment and retention, and to delivering compassionate patient care. This systematic review aims to explore factors affecting personal resilience among psychiatrists, in particular, those that may impair well-being and those that facilitate resilience practice. A literature search was performed of the Ovid®, Embase®, CINAHL and PsycINFO databases, using keywords to identify empirical studies involving psychiatrists that examined resilience, stress and burnout from the past 15 years.ResultsThirty-three international English language studies were included, showing that a combination of workplace, personal and non-workplace factors negatively and positively influenced well-being and resilience.Clinical implicationsGiven that workplace factors were the most commonly cited, it would appear that any resilience package that predominantly targets interventions at the workplace level would be particularly fruitful. Future research, however, needs to address the absence of a universal measurement of well-being and its moderators so that any potential interventions are better evaluated.
The diverse neural crest: from embryology to human pathology
Authors: Etchevers Heather C. , Dupin Elisabeth , Le Douarin Nicole M. 
Published: March 2019

ABSTRACTWe review here some of the historical highlights in exploratory studies of the vertebrate embryonic structure known as the neural crest. The study of the molecular properties of the cells that it produces, their migratory capacities and plasticity, and the still-growing list of tissues that depend on their presence for form and function, continue to enrich our understanding of congenital malformations, paediatric cancers and evolutionary biology. Developmental biology has been key to our understanding of the neural crest, starting with the early days of experimental embryology and through to today, when increasingly powerful technologies contribute to further insight into this fascinating vertebrate cell population.
A core mechanism for specifying root vascular patterning can replicate the anatomical variation seen in diverse plant species
Authors: Mellor Nathan , Vaughan-Hirsch John , Kümpers Britta M. C. , Help-Rinta-Rahko Hanna , Miyashima Shunsuke , Mähönen Ari Pekka , Campilho Ana , King John R. , Bishopp Anthony 
Published: March 2019

ABSTRACT
Pattern formation is typically controlled through the interaction between molecular signals within a given tissue. During early embryonic development, roots of the model plant Arabidopsis thaliana have a radially symmetric pattern, but a heterogeneous input of the hormone auxin from the two cotyledons forces the vascular cylinder to develop a diarch pattern with two xylem poles. Molecular analyses and mathematical approaches have uncovered the regulatory circuit that propagates this initial auxin signal into a stable cellular pattern. The diarch pattern seen in Arabidopsis is relatively uncommon among flowering plants, with most species having between three and eight xylem poles. Here, we have used multiscale mathematical modelling to demonstrate that this regulatory module does not require a heterogeneous auxin input to specify the vascular pattern. Instead, the pattern can emerge dynamically, with its final form dependent upon spatial constraints and growth. The predictions of our simulations compare to experimental observations of xylem pole number across a range of species, as well as in transgenic systems in Arabidopsis in which we manipulate the size of the vascular cylinder. By considering the spatial constraints, our model is able to explain much of the diversity seen in different flowering plant species.
Microtubule acetylation but not detyrosination promotes focal adhesion dynamics and astrocyte migration
Authors: Bance Bertille , Seetharaman Shailaja , Leduc Cécile , Boëda Batiste , Etienne-Manneville Sandrine 
Published: March 2019

ABSTRACT
Microtubules play a crucial role in mesenchymal migration by controlling cell polarity and the turnover of cell adhesive structures on the extracellular matrix. The polarized functions of microtubules imply that microtubules are locally regulated. Here, we investigated the regulation and role of two major tubulin post-translational modifications, acetylation and detyrosination, which have been associated with stable microtubules. Using primary astrocytes in a wound healing assay, we show that these tubulin modifications are independently regulated during cell polarization and differently affect cell migration. In contrast to microtubule detyrosination, αTAT1 (ATAT1)-mediated microtubule acetylation increases in the vicinity of focal adhesions and promotes cell migration. We further demonstrate that αTAT1 increases focal adhesion turnover by promoting Rab6-positive vesicle fusion at focal adhesions. Our results highlight the specificity of microtubule post-translational modifications and bring new insight into the regulatory functions of tubulin acetylation.
This article has an associated First Person interview with the first author of the paper.
A nuclear localization signal targets tail-anchored membrane proteins to the inner nuclear envelope in plants
Authors: Groves Norman R. , McKenna Joseph F. , Evans David E. , Graumann Katja , Meier Iris 
Published: March 2019

ABSTRACT
Protein targeting to the inner nuclear membrane (INM) is one of the least understood protein targeting pathways. INM proteins are important for chromatin organization, nuclear morphology and movement, and meiosis, and have been implicated in human diseases. In opisthokonts, one mechanism for INM targeting is transport factor-mediated trafficking, in which nuclear localization signals (NLSs) function in nuclear import of transmembrane proteins. To explore whether this pathway exists in plants, we fused the SV40 NLS to a plant ER tail-anchored protein and showed that the GFP-tagged fusion protein was significantly enriched at the nuclear envelope (NE) of leaf epidermal cells. Airyscan subdiffraction limited confocal microscopy showed that this protein displays a localization consistent with an INM protein. Nine different monopartite and bipartite NLSs from plants and opisthokonts, fused to a chimeric tail-anchored membrane protein, were all sufficient for NE enrichment, and both monopartite and bipartite NLSs were sufficient for trafficking to the INM. Tolerance for different linker lengths and protein conformations suggests that INM trafficking rules might differ from those in opisthokonts. The INM proteins developed here can be used to target new functionalities to the plant nuclear periphery.
This article has an associated First Person interview with the first author of the paper.
Nuclear speckle fusion via long-range directional motion regulates speckle morphology after transcriptional inhibition
Authors: Kim Jiah , Han Kyu Young , Khanna Nimish , Ha Taekjip , Belmont Andrew S. 
Published: March 2019

ABSTRACT
Although the formation of RNA-protein bodies has been studied intensively, their mobility and how their number and size are regulated are still poorly understood. Here, we show significantly increased mobility of nuclear speckles after transcriptional inhibition, including long-range directed motion of one speckle towards another speckle, terminated by speckle fusion, over distances up to 4 µm and with velocities between 0.2 µm/min and 1.5 µm/min. Frequently, three or even four speckles follow very similar paths, with new speckles appearing along the path followed by a preceding speckle. Speckle movements and fusion events contribute to fewer, but larger, speckles after transcriptional inhibition. These speckle movements are not actin dependent, but occur within chromatin-depleted channels enriched with small granules containing the speckle marker protein SON. Similar long-range speckle movements and fusion events were observed after heat shock or heavy metal stress, and during late G2 and early prophase. Our observations suggest a mechanism for long-range, directional nuclear speckle movements, contributing to overall regulation of nuclear speckle number and size as well as overall nuclear organization.
This article has an associated First Person interview with the first author of the paper.
Characterization of myofibroblasts isolated from the intestine of patients with inflammatory bowel disease
Authors: Dionne Serge , Restellini Sophie , Koenekoop Jamie , Salvador Escribano Pedro , Piccirillo Ciriaco A. , Charlebois Patrick , Liberman A. Sender , Stein Barry , Duchatellier Carl Frederic , Seidman Ernest Gerald 
Published: March 2019

Background: Intestinal fibrosis represents a serious complication of inflammatory bowel diseases (IBD), often necessitating surgical resections. Myofibroblasts are primarily responsible for interstitial matrix accumulation in fibrotic diseases. However intestinal myofibroblasts (IMF) remain inadequately characterized. The aim was to examine fibroblast markers and fibrosis-associated gene expression in IMF isolated from resected intestine from IBD and control patients. As well as determining the effect of the fibrogenic cytokine TGFβ. Methods: Intestinal resections were obtained (n =35) from consenting patients undergoing elective surgery (2014-16). Primary cultures of IMF were isolated using DTT and EDTA and cultured. Viability and phenotypic characterization of IMF was carried out by flow cytometry and fluorescence microscopy. IMF (passages 3-8) were treated for 24 hours. Cytokines were quantified in IMF by real time PCR and in supernatants using the human pro-inflammatory cytokine panel Results: All markers and most fibrosis mediators studied were preferentially expressed by IMF compared to mucosal tissue. Metalloproteinases (MMP) 2 and 3, as well as their inhibitor TIMP1, are highly expressed by IMF. They also highly expressed inflammatory mediators, including IL-6, IL-8, CCL2 and PTGS2. Whereas mucosal expression of pro-inflammatory cytokines such as TNFα and IL-17 is increased in IBD, that of fibrosis mediators was not different. Fibrosis-related gene expression in IMF from IBD patients and controls was similar, but IMF from IBD expressed higher levels of several inflammatory genes. IMF from CD and UC had mostly similar expression profiles. TGFβ induced expression of fibrogenic genes αSMA, COL1A1, CTGF, FN1 and LOX. TGFβ-stimulated IMF released increased levels of IL-6, whereas IL-6, IL-8, as well as small amounts of IFN-γ and IL12p70 were produced following stimulation with IL-1β+IL-23. Conclusions: This study extends knowledge about the pathogenesis of fibrosis in IBD. Further research in the identification of mechanisms involved in IMF activation and fibrogenesis are required.
The approach to an isolated close anterior margin in breast conserving surgery
Authors: O’Connell L , Walsh S , Evoy D , O’Doherty A , Quinn C , Rothwell J , Geraghty J , McDermott EW , Prichard R 
Published: March 2019

Introduction Although close radial margins after breast-conserving surgery routinely undergo re-excision, appropriate management of patients with close anterior margins remains a topic of controversy. An increasing body of literature suggests that re-excision of close anterior margins yields low rates of residual malignancy and may only be necessary in selected patients. The aim of this study was to examine the management of close anterior margins after breast conserving surgery in a single institution and to analyse the rate of residual disease in re-excised anterior margins. Methods All patients having breast conserving surgery at St Vincent’s University Hospital from January 2008 to December 2012 were reviewed retrospectively. Data collected included patient demographics, tumour characteristics, margin positivity, re-excision rates and definitive histology of the re-excision specimens. A close margin was defined as les than 2 mm. Results A total of 930 patients were included with an average age of 65 years (range 29–94 years). Of these, 121 (13%) had a close anterior margin. Further re-excison of the anterior margin was carried out in 37 patients (30.6%) and a further 16 (13.2%) proceeded to mastectomy. Residual disease was found in 18.5% (7/36) of those who underwent re-excision and 7/16 (43.75%) of those who underwent mastectomy. Overall, 11.57% (14/121) of patients with close anterior margins were subsequently found to have residual disease. Conclusion The low yield of residual disease in re-excised anterior margins specimens supports the concept that routine re-excision of close anterior margins is not necessary. Further research is required to definitively assess its influence on the risk of local recurrence.
Trends in paediatric distal radius fractures: an eight-year review from a large UK trauma unit
Authors: Mamoowala N , Johnson NA , Dias JJ 
Published: March 2019

Introduction This observational study investigated the incidence of distal radius fractures in children, to determine whether the rate is rising, the effect of seasonal variation on incidence and whether fracture type and rate of surgical intervention has changed, to help in determining costs for secondary care and to aid resource allocation. Materials and methods All paediatric patients(n = 6529) who sustained a distal radius fracture over an eight-year period (2007–2014) were identified. Poisson regression modelling was used to identify change in trends. Results There was no change in distal radius fracture incidence, rate of surgical intervention (P = 0.36) or fracture type (P = 0.70). Overall incidence was 337 fractures per 100,000 patient/years. The highest fracture incidence was seen in older school boys (708 per 100,000 patient/years, P < 0.005). Overall fracture rate was lower in winter (P < 0.005). Incidence is highest in summer and the main variation is related to season. Discussion These data can help to predict accurately the number of children presenting to the emergency department with wrist fractures depending on the time of year.
Full-Size and Partially Truncated Cardiac Troponin Complexes in the Blood of Patients with Acute Myocardial Infarction
Authors: Vylegzhanina Alexandra V , Kogan Alexander E , Katrukha Ivan A , Koshkina Ekaterina V , Bereznikova Anastasia V , Filatov Vladimir L , Bloshchitsyna Marina N , Bogomolova Agnessa P , Katrukha Alexey G 
Published: March 2019

AbstractBACKGROUNDThe measurement of cardiac isoforms of troponin I (cTnI) and troponin T (cTnT) is widely used for the diagnosis of acute myocardial infarction (AMI). However, there are conflicting data regarding what forms of cTnI and cTnT are present in the blood of AMI patients. We investigated cTnI and cTnT as components of troponin complexes in the blood of AMI patients.METHODSGel filtration techniques, sandwich fluoroimmunoassays, and Western blotting were used.RESULTSPlasma samples from patients with AMI contained the following troponin complexes: (a) a cTnI-cTnT-TnC complex (ITC) composed of full-size cTnT of 37 kDa or its 29-kDa fragment and full-size cTnI of 29 kDa or its 27-kDa fragments; (b) ITC with lower molecular weight (LMW-ITC) in which cTnT was truncated to the 14-kDa C-terminal fragments; and (c) a binary cTnI-cTnC complex composed of truncated cTnI of approximately 14 kDa. During the progression of the disease, the amount of ITC in AMI samples decreased, whereas the amounts of LMW-ITC and short 16- to 20-kDa cTnT central fragments increased. Almost all full-size cTnT and a 29-kDa cTnT fragment in AMI plasma samples were the components of ITC. No free full-size cTnT was found in AMI plasma samples. Only 16- to 27-kDa central fragments of cTnT were present in a free form in patient blood.CONCLUSIONSA ternary troponin complex exists in 2 forms in the blood of patients with AMI: full-size ITC and LMW-ITC. The binary cTnI-cTnC complex and free cTnT fragments are also present in patient blood.
Restorative strategies for weakened roots: Systematic review and Meta-analysis of in vitro studies
Authors: Corrêa Gislene , Pradebon Brondani Lucas , Sarkis-Onofre Rafael , Dalmolin Bergoli César 
Published: March 2019

Objective: This study evaluated fracture resistance values and failure mode of weakened roots restored with cast post and core, fiber post and anatomical posts. Materials and methods: In vitrostudies, published in English, Portuguese and Spanish, that evaluated weakened roots restored with at least two of mentioned strategies were searched for in three databases MEDLINE, PubMed and Scopus until June 2017; there was no restriction regarding the publication year. Fracture resistance values were obtained, and the following comparisons: cast post and core vs. fiber post and vs. anatomical post and fiber post vs. anatomical post were performed. Meta-analyses were conducted using the random effects model to calculate the combined effect of the difference between the averages. Six articles were included in the study. Results: There was no significant difference between the fracture resistance values, but more catastrophic failures were observed on cast post and core, and the included studies presented a low risk of bias for the most domains. Conclusion: It was concluded that weakened roots restored with the searched strategies seem to have similar fracture strength, but fiber and anatomic post decreases the possibility of catastrophic failure.KeywordsReview; Root; Dental restoration.
A política da catástrofe: melancolia e despolitização da história na obra de W. G. Sebald
Authors: Guidée Raphaëlle 
Published: March 2019

Sintomas da inflexão melancólica que domina as artes da memória no fim do século XX, as narrativas do escritor alemão W. G Sebald se assemelham a um registro inesgotável das devastações causadas ou sofridas pela espécie humana. Nenhum progresso, senão no aperfeiçoamento das ferramentas de destruição; nenhum sentido, exceto no eterno retorno do desastre que faz do infortúnio a característica de uma “espécie desesperada”. Mas o quão é bom exumar os traços das vítimas da história se é impossível esperar acabar com a violência por elas sofrida? Por que ressuscitar os oprimidos cuja história apenas repete a derrota irreparável? Examinando o diálogo continuo entre a obra de Sebald e as teses “sobre o conceito de história” (1940), de Walter Benjamin, este artigo demonstra os riscos de despolitização de uma filosofia fundada no valor revolucionário da memória dos vencidos e tenta, portanto, esclarecer a tensão entre ética e política que atravessa um grande número de produções memoriais contemporâneas.
palavras clave: Sebald; melancolia; despolitização.