bluuebunny/crossref_metadata_2025_split huggingface dataset:
ПЕРСПЕКТИВЫ ИСПОЛЬЗОВАНИЯ ДРОЖЖЕВОГО ДИСПЛЕЯ В?БИОТЕХНОЛОГИИ И КЛЕТОЧНОЙ БИОЛОГИИ (ОБЗОР), "Прикладная биохимия и микробиология"
Authors: Падкина М.В. , Самбук Е.В. 
Published: August 2018

Технология дрожжевого дисплея, появившаяся 20 лет назад и основанная на экспонировании на поверхности клетки целевых белков за счет их слияния с белками клеточной стенки, находит все более широкое применение в фундаментальных и прикладных исследованиях. К основному преимуществу поверхностного дисплея на основе эукариотических микроорганизмов - дрожжей относится возможность корректной модификации белков млекопитающих. Поверхностный дисплей является одним из инструментов изучения функций белков, межбелковых взаимодействий, анализа и селекции пептидных и белковых библиотек. Данная технология позволяет получать клетки дрожжей, которые обладают такими новыми свойствами, как каталитическая активность, способность связывать определенные лиганды, в том числе, редкие и тяжелые металлы. Это дает возможность использовать дрожжи в биотехнологии, а также для биоремедиации и биомониторинга окружающей среды. В обзоре рассмотрены способы получения поверхностного дисплея на основе дрожжей Saccharomyces cerevisiae, Pichia pastoris и Yarrowia lipolytica, свойства якорных белков и основные области применения технологии дрожжевого дисплея.
КЛОНИРОВАНИЕ, ВЫДЕЛЕНИЕ И?ИЗУЧЕНИЕ СВОЙСТВ НОВОЙ ГОМОЛОГИЧНОЙ ЭКЗО-АРАБИНАЗЫ ГРИБА PENICILLIUM CANESCENS, "Прикладная биохимия и микробиология"
Authors: Семёнова М.В. , Волков П.В. , Рожкова А.М. , Зоров И.Н. , Синицын А.П. 
Published: August 2018

Исследован новый фермент экзо-арабиназа (GH93, экзо-АБН), продуцируемый аскомицетным грибом Penicillium canescens. В результате клонирования гена abn1, кодирующего экзо-АБН, в штамм-реципиент P. canescens RN3-11-7 получены рекомбинантные штаммы-продуценты, способные с высоким выходом продуцировать внеклеточную экзо-АБН (20-30% от общего количества внеклеточного белка). С помощью хроматографических методов была получена гомогенная по данным электрофореза в ПААГ с Na-ДДС экзо-АБН с молекулярной массой 47 кДа. Фермент обладал высокой удельной активностью по отношению к линейному арабинану (117 ед./мг), низкой - к разветвленному арабинану и арабиноксилану (4-5 ед/мг), а также пара-нитрофенил- -L-арабинофуранозиду (0.3 ед/мг), не гидролизовал арабиногалактан и пара-нитрофенил- -L-арабинопиранозид - субстраты, содержаще арабинозу в пиранозной форме. Основным продуктом гидролиза линейного арабинана была арабиногексаоза. Экзо-АБН имела рН-оптимум 5.0, температурный оптимум - 60 °C, была стабильна в широком диапазоне рН 4.0-7.0 и при нагревании до 50 °C в течение 180 мин. Проведен глубокий гидролиз линейного и разветвленного арабинанов под действием смесей экзо- и эндо-арабиназ, арабинофуранозидазы и арабинофуран-арабиноксилангидролазы. Достигнута степень конверсии субстратов 67 и 83% соответственно от максимально возможной.
СТРОЕНИЕ, ВОЗРАСТ И ОБСТАНОВКИ ФОРМИРОВАНИЯ ОРДОВИКСКИХ КОМПЛЕКСОВ СЕВЕРО-ЗАПАДНОГО ОБРАМЛЕНИЯ КОКЧЕТАВСКОГО МАССИВА, СЕВЕРНЫЙ КАЗАХСТАН, "Стратиграфия. Геологическая корреляция"
Authors: Дегтярев К.Е. , Толмачева Т.Ю. , Третьяков А.А. , Кузнецов Н.Б. , Белоусова Е.А. , Романюк Т.В. 
Published: August 2018

Рассмотрены данные о строении, возрасте и обстановках формирования ордовикских осадочных и вулканогенно-осадочных комплексов Стерлитамакской, Марьевской и Иманбурлукской структурно-формационных зон западного и северо-западного обрамления Кокчетавского массива Северного Казахстана. Приведены результаты детальных стратиграфических, геохимических и геохронологических исследований опорного разреза ордовика Марьевской зоны. Изученный разрез сложен карбонатными, терригенными, реже вулканогенно-осадочными породами значительного стратиграфического интервала от тремадокского яруса нижнего ордовика до нижней части сандбийского яруса верхнего ордовика. Впервые по конодонтам был установлен ранне-среднеордовикский возраст наиболее древней известняково-доломитовой толщи, ранее условно относившейся к кембрию. Залегающая выше туфогенно-терригенная куприяновская свита теперь относится только к среднему ордовику. Особенности состава литокластических туфов, слагающих ее среднюю часть, позволяют предполагать их формирование в пределах островной дуги. Известняки из низов наиболее молодой терригенно-карбонатной крещеновской свиты отнесены к нижней части сандбийского яруса верхнего ордовика. Изучение возрастов обломочных цирконов из терригенных пород известняково-доломитовой толщи показало, что наиболее вероятным источником сноса при ее формировании являлись ранненеопротерозой­- ские кварцито-сланцевые толщи Кокчетавского массива. Установлено, что на протяжении ордовика в северо-западном обрамлении Кокчетавского массива происходила смена обстановок осадконакопления от закрытых лагун к морскому относительно глубоководному бассейну с нормальной соленостью и активной циркуляцией водных масс. На протяжении этого времени отмечался достаточно высокий уровень терригенного сноса, отразившийся в формировании толщ значительной мощности. Общий тренд к углублению обстановок осадконакопления от раннего к позднему ордовику прерывался периодическими поднятиями уровня моря в дапинское и раннедарривильское время.
Ni/NiO/HfO2 Core/Multishell Nanowire ReRAM Devices with Excellent Resistive Switching Properties
Authors: Huang Ting‐Kai , Chen Jui‐Yuan , Ting Yi‐Hsin , Wu Wen‐Wei 
Published: August 2018

AbstractResistive random‐access memory (ReRAM) is one of the most promising types of nonvolatile memory because it has several important advantages, for example, a simple metal‐insulator‐metal structure, fast operating speed, high endurance, high retention, and low energy consumption. However, the reliability of ReRAM nanodevices is not persistent, and the complete switching mechanism is not fully understood. In this study, a unique 1D Ni/NiO/HfO2 core/multishell ReRAM nanodevice is designed and fabricated. The different properties, including the electrical characteristics, surface morphology, and elemental distribution, are systematically investigated. The Ni/NiO/HfO2 heterostructure nanowire ReRAM device exhibits excellent electrical characteristics and resistive switching properties. It is remarkable that the endurance could be maintained up to 200 cycles, which is extremely good for a 1D ReRAM device. Additionally, a focused‐ion beam technique is used to prepare samples for subsequent transmission electron microscopic (TEM) observation. From the TEM analysis, the position of the conducting filaments is verified and the elemental composition of the conducting filaments is confirmed. The migration of hafnium ions forms the conducting filaments between the HfO2 layer and Ni core, resulting in the switching characteristic. The study enriches the understanding of the mechanism and provides a design to enhance the resistive switching properties of ReRAM nanodevices.
Synthesis and Properties of Hydrazine‐Embedded Biphenothiazines and Application of Hydrazine‐Embedded Heterocyclic Compounds to Fluorescence Cell Imaging
Authors: Shindo Yuri , Nomura Shogo , Saikawa Yoko , Nakata Masaya , Tanaka Katsunori , Hanaya Kengo , Sugai Takeshi , Higashibayashi Shuhei 
Published: August 2018

AbstractHydrazine‐embedded unsubstituted butterfly‐shaped biphenothiazine and its sulfoxides were synthesized by dimerization of 1,9‐dibromophenothiazine, which was prepared by realizing selective debromination at the 3,7‐positions of 1,3,7,9‐tetrabromophenothiazine. cis/trans‐Biphenothiazine sulfoxide was selectively prepared by changing the oxidation temperature to control the inversion rate of the butterfly shape of the intermediate. Their butterfly‐shapes, conformations, photophysical properties (UV‐vis absorption, fluorescence), and redox properties were elucidated by X‐ray analysis, DFT calculations, spectral and electrochemical measurements. Fluorescent hydrazine‐embedded biphenothiazine sulfoxides and bicarbazoles were applied to cell imaging of HeLa cells. The bicarbazoles exhibited high fluorescence signals in the cells with low toxicity.
Aboriginal and Torres Strait Islander employment policy and Welfare to Work: The Community Development Programme and the need for new narratives, new alliances and new institutions
Authors: Jordan Kirrily 
Published: August 2018

AbstractThe way Australian federal governments have approached Aboriginal and Torres Strait Islander employment and welfare policy over the last two decades has been a paradigmatic example of what not to do in policymaking. In the absence of effective engagement or consultation, a series of decisions under Coalition and Australian Labor Party governments have had a range of negative consequences for Aboriginal and Torres Strait Islander peoples. These changes – centred around the closure of the Community Development Employment Projects (CDEP) scheme and implementation of the Community Development Programme (CDP) – have resulted in increased unemployment, inadequate support for job creation, a dramatic over‐application of income penalties to social security recipients and reduced capacity in many community organisations. This article argues that these outcomes constitute policy failure, especially given that genuine engagement with Aboriginal and Torres Strait Islander people could have prevented many of these consequences. It then turns to an exploration of three ways in which Aboriginal and Torres Strait Islander people are responding to such policy failure: through promotion of new narratives, new alliances, and new institutions. The study concludes that while we should avoid “specious hope”, there is room for optimism in a renewed push for Aboriginal and Torres Strait Islander sovereignty.
Green transformations in Vietnam's energy sector
Authors: Urban Frauke , Siciliano Giuseppina , Wallbott Linda , Lederer Markus , Dang Nguyen Anh 
Published: August 2018

AbstractVietnam has experienced rapid economic growth over the past few decades, as well as growing environmental pressures. The country is therefore pursuing strategies for green transformations, which are the processes of restructuring to bring economies and societies within the planetary boundaries. This article addresses the opportunities, barriers, and trade‐offs for green transformations in Vietnam's energy sector and examines them from an energy justice perspective. The article draws on in‐depths expert interviews with representatives from government agencies, private firms, academic institutions, and multilateral institutions in Vietnam. The article finds that Vietnam is undergoing efforts to move away from business as usual by promoting renewable energy and energy efficiency, as well as aligning energy and climate plans with national development priorities such as energy security and economic growth. Yet there is a need for more coordinated, integrated approaches and policies that span across the 3 areas that address green transformations in Vietnam: green growth, sustainable development, and climate change. Finally, although key actors seem to be aware and may be critical of major trade‐offs such as land grabs for energy projects, the impacts on affected people need to be better understood and mitigated.
Amphiphilic Carbazole‐Containing Compounds with Lower Critical Solution Temperature Behavior for Supramolecular Self‐Assembly and Solution‐Processable Resistive Memories
Authors: Li Xiaoying , Hong Eugene Yau‐Hin , Chan Alan Kwun‐Wa , Poon Chun‐Ting , Li Bao , Wu Lixin , Yam Vivian Wing‐Wah 
Published: August 2018

AbstractThe self‐organization and resistive memory performances of a series of newly synthesized water‐soluble amphiphilic carbazole derivatives have been explored. Temperature‐dependent UV/Vis absorption spectroscopy has been conducted to study the isodesmic self‐assembly mechanism of the carbazole‐containing compounds. This class of compounds also exhibits interesting lower critical solution temperature properties, which are sensitive to concentration and ionic additives. One of the compounds has been solution‐processed and utilized as an active material in the engineering of resistive memory devices, exhibiting a switching voltage of about 3.9 V, a constant ON/OFF current ratio of 106, and a long retention time of 104 s. The present work demonstrates the versatile potential applications of water‐soluble amphiphilic carbazole‐containing compounds in supramolecular chemistry and resistive memory devices.
A randomized controlled trial evaluating the Hebrew adaptation of the PEERS® intervention: Behavioral and questionnaire‐based outcomes
Authors: Rabin Shai Joseph , Israel‐Yaacov Sandra , Laugeson Elizabeth A. , Mor‐Snir Irit , Golan Ofer 
Published: August 2018

Social interaction deficits form a core characteristic of ASD that is commonly targeted through social‐skill groups. The Program for the Education and Enrichment of Relational Skills (PEERS®) is a well‐established parent‐assisted intervention for adolescents, which addresses key areas of social functioning. PEERS® has been mainly studied in North‐America and its evaluations were mostly questionnaire based. The aim of the current study was to test the effectiveness of the adapted and translated Hebrew version of the PEERS® intervention in a randomized controlled trial, using behavioral measures of peer interaction, in addition to self, parent, and teacher reports. Forty‐one participants with ASD and no intellectual impairment, aged 12–17 years, were randomly assigned to an immediate intervention or a delayed‐intervention group. All participants were assessed before and after the immediate intervention, and again at follow up, after the delayed intervention took place. Results revealed intervention‐related behavioral improvements on adolescents’ engagement, question‐asking, and physical arousal. Parental reports indicated improved social skills, and reduced ASD symptoms. Adolescents reported on more social encounters, greater empathy, and scored higher on social‐skill knowledge. Most of these effects maintained at a 16‐week follow‐up. Teacher reports' yielded effects only on pre‐post intervention analysis. Adolescents’ improvement on behavioral engagement predicted parent‐reported social skills improvement. Our findings support the effectiveness of the adapted Hebrew version of PEERS® for adolescents with ASD, through significant behavioral and questionnaire‐based outcomes, which maintained at follow‐up. Autism Res 2018, 11: 1187–1200. © 2018 International Society for Autism Research, Wiley Periodicals, Inc.Lay SummarySocial‐skills groups, which facilitate key social deficits characteristic of ASD, are a popular intervention for adolescents with ASD. Indeed, many treatment protocols have been published, and some have also been research validated. However, there have been inconsistent findings regarding the effectiveness of different protocols, in addition to limited findings of improvement beyond questionnaire reports. This study evaluated the Hebrew adaptation of the PEERS® intervention, a 16‐weeks long program, which involves the parents as their adolescents’ social coaches. Following the intervention, adolescents improved their social‐skills, participated more in social encounters, reported greater empathy, and demonstrated higher social‐skill knowledge. A live play‐role assessment with an unfamiliar peer indicated that adolescents showed greater involvement, asked more questions and were more physically relaxed during the conversation. Improvements maintained 16 weeks after the intervention was completed.
Sensitivity of multiple life stages of 2 freshwater mussel species (Unionidae) to various pesticides detected in Ontario (Canada) surface waters
Authors: Salerno Joseph , Bennett Charles J. , Holman Emily , Gillis Patricia L. , Sibley Paul K. , Prosser Ryan S. 
Published: August 2018

Abstract

Freshwater mussels contribute important ecological functions to aquatic systems. The water filtered by mussel assemblages can improve water quality, and the mixing of sediments by burrowing mussels can improve oxygen content and release nutrients. However, nearly 70% of North American freshwater mussel species are listed as either endangered, threatened, or in decline. In Ontario, 28 species are in decline or in need of protection. Even though freshwater mussels have a heightened sensitivity to some contaminants, few studies have investigated the risks that various pesticide classes pose to one freshwater mussel species or among life stages. Lampsilis siliquoidea and Villosa iris were the focus of the present study, with the latter currently listed as of “special concern” in Canada. A potential risk to the recovery of freshwater mussel species is the presence and persistence of pesticides in Ontario surface waters. Acute (48 h) toxicity tests were performed with V. iris glochidia to determine the effect on viability (surrogate for survival) following exposure to 4 fungicides (azoxystrobin, boscalid, metalaxyl, and myclobutanil), 3 neonicotinoids (clothianidin, imidacloprid, and thiamethoxam), 2 carbamates (carbaryl and malathion), 1 organophosphate (chlorpyrifos), and 1 butenolide (flupyradifurone). Juvenile and adult L. siliquoidea were also exposed to azoxystrobin, clothianidin, imidacloprid (juvenile only), and carbaryl (adult only). Our study found in general that all life stages were insensitive to the pesticides tested, with median effect and lethal concentrations &gt;161 µg/L. The pesticides tested likely represent a minimal risk (hazard quotients &lt;5.4 × 10−3) to freshwater mussel viability and survival in acute (48 h) and subchronic (28 d) exposures, respectively, in Ontario streams where pesticide concentrations were considerably lower than those tested in the present study. Environ Toxicol Chem 2018;37:2871–2880. © 2018 SETAC
Organochlorines, perfluoroalkyl substances, mercury, and egg incubation temperature in an Arctic seabird: Insights from data loggers
Authors: Blévin Pierre , Shaffer Scott A. , Bustamante Paco , Angelier Frédéric , Picard Baptiste , Herzke Dorte , Moe Børge , Gabrielsen Geir Wing , Bustnes Jan Ove , Chastel Olivier 
Published: August 2018

Abstract

In birds, incubation-related behaviors and brood patch formation are influenced by hormonal regulation such as prolactin secretion. Brood patch provides efficient heat transfer between the incubating parent and the developing embryo in the egg. Importantly, several environmental contaminants are already known to have adverse effects on avian reproduction. However, relatively little is known about the effect of contaminants on incubation temperature (Tinc) in wild birds. By using temperature thermistors placed into artificial eggs, we investigated whether the most contaminated parent birds are less able to provide appropriate egg warming and thus less committed to incubating their clutch. Specifically, we investigated the relationships among 3 groups of contaminants (organochlorines, perfluoroalkyl substances [PFASs], and mercury [Hg]) with Tinc and also with prolactin concentrations and brood patch size in incubating Arctic black-legged kittiwakes (Rissa tridactyla). Our results reveal that among the organochlorines considered, only blood levels of oxychlordane, the main metabolite of chlordane, a banned pesticide, were negatively related to the minimum incubation temperature in male kittiwakes. Levels of PFASs and Hg were unrelated to Tinc in kittiwakes. Moreover, our study suggests a possible underlying mechanism: since we reported a significant and negative association between blood oxychlordane concentrations and the size of the brood patch in males. Finally, this reduced Tinc in the most oxychlordane-contaminated kittiwakes was associated with a lower egg hatching probability. Environ Toxicol Chem 2018;37:2881–2894. © 2018 SETAC
Des Städters neue Lust
Authors: Fenske Michaela 
Published: August 2018

AbstractFaszination Bienen, jetzt auch im urbanen Raum: Immer mehr Stadtbewohner in Europa und Nordamerika imkern in ihrer Freizeit. In Berlin etwa lassen sich dabei Denk‐ und Verhaltensweisen beobachten, die zwischen ökologischem Anspruch, Mensch‐Tier‐Interaktionen und einer neuen Selbst‐ und Umweltwahrnehmung angesiedelt sind.
Gray matter bases of psychotic features in adult bipolar disorder: A systematic review and voxel‐based meta‐analysis of neuroimaging studies
Authors: Wang Xiuli , Tian Fangfang , Wang Song , Cheng Bochao , Qiu Lihua , He Manxi , Wang Hongming , Duan Mingjun , Dai Jing , Jia Zhiyun 
Published: August 2018

AbstractPsychotic bipolar disorder (P‐BD) is a specific subset that presents greater risk of relapse and worse outcomes than nonpsychotic bipolar disorder (NP‐BD). To explore the neuroanatomical bases of psychotic dimension in bipolar disorder (BD), a systematic review was carried out based on the gray matter volume (GMV) among P‐BD and NP‐BD patients and healthy controls (HC). Further, we conducted a meta‐analysis of GMV differences between P‐BD patients and HC using a whole‐brain imaging approach. Our review revealed that P‐BD patients exhibited smaller GMVs mainly in the prefronto‐temporal and cingulate cortices, the precentral gyrus, and insula relative to HC both qualitatively and quantitatively. Qualitatively the comparison between P‐BD and NP‐BD patients suggested inconsistent GMV alterations mainly involving the prefrontal cortex, while NP‐BD patients showed GMV deficits in local regions compared with HC. The higher proportions of female patients and patients taking psychotropic medication in P‐BD and P‐BD type I were associated with smaller GMV in the right precentral gyrus, and the right insula, respectively. In conclusions, psychosis in BD might be associated with specific cortical GMV deficits. Gender and psychotropic medication might have effects on the regional GMVs in P‐BD patients. It is necessary to distinguish psychotic dimension in neuroimaging studies of BD.
Bioavailability of mercury in power plant wastewater and ambient river samples: Evidence that the regulation of total mercury is not appropriate
Authors: Reash Robin J 
Published: August 2018

Abstract

Mercury (Hg) is a neurotoxin that can cause debilitating effects to human and environmental receptors under high exposure conditions. For industrial and municipal point sources that discharge Hg, wastewater limitations on total Hg (THg) concentrations or loads are typical. While this regulatory practice provides simplicity for regulated industry and water resource agencies (i.e., for analytical detection and reporting purposes), it ignores the important considerations of speciation and bioavailability. In this study, water samples were collected from multiple power plant wastewater, simulated mixing zone, and ambient river locations (N = 10 to 20) and were analyzed for bioavailable Hg forms (methylmercury and acid-labile Hg, or BHg), THg, and dissolved Hg. The median concentration of THg in wastewater, mixing zone, and ambient river samples was 7.1, 5.3, and 2.3 ng/L, respectively. The percentages of THg as BHg (median values) were 18.7%, 29.3%, and 8.5% for wastewater, mixing zone, and ambient river samples. The percentages of methylmercury (MeHg) as THg were not statistically different between paired ambient and mixing zone samples (P &gt; 0.05); this result indicates that wastewater did not increase the MeHg fraction when mixed with ambient water. Multiple regression analysis indicated that variation in THg for combined wastewater and mixing zone samples could be adequately explained by pooled water quality parameters (total suspended solids, total dissolved solids, sulfate, total organic carbon, pH, specific conductivity; r2 = 0.51; P &lt; 0.05); however, no significant regression relationships were apparent for the percentage of BHg. These results, at least for the wastewater samples evaluated, indicate that regulating THg is likely overly conservative, and mechanisms to regulate the bioavailable forms of Hg are needed. If Hg fish tissue monitoring data indicate that concentrations are less than consumption thresholds, metal translator methodologies or bioavailability-based criterion techniques (as currently used for non-Hg trace elements) should be allowed for Hg. Integr Environ Assess Manag 2019;15:142–147. © 2018 SETAC