bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lower 2D:4D digit ratio is associated with reduced impulse control in women
Authors: Rodríguez-Ramos Ángel , Moriana Juan Antonio , García-Torres Francisco , Ruiz-Rubio Manuel 
Published: September 2019

Abstract
Sex hormones induce sexual differentiation of the brain which might result in sex-specific behaviors. 2D:4D digit ratio is commonly used as a marker of the balance between prenatal testosterone and estrogen. In this study we analyzed if there was an association between 2D:4D and personality in women. We found that 2D:4D might be related with emotional stability, and specifically with its subdimension impulse control; while no associations were found with the dimensions energy, friendliness, conscientiousness and openness.
Temporal expression patterns of Pasteuria spp. sporulation genes
Authors: Dyrdahl-Young Ruhiyyih , Hu Weiming , DiGennaro Peter 
Published: September 2019

Abstract
Endospore-forming bacterium in the genus Pasteuria spp. infect multiple agriculturally significant plant parasitic nematodes and has potential as a potent biological control. Success as a biological control requires not only spore attachment to the cuticle, but sporulation and reproduction within the nematode host. Tracking and identifying Pasteuria spp. development is then critical to demonstrating efficacy as a biocontrol. Microscopic observations suggest Pasteuria spp. follows the model bacterium, Bacillus subtilis, sporulation. Here, we identified B. subtilis homologs of sporulation regulators in Pasteuria spp. and characterized the temporal expression of these genes throughout the bacterium’s ∼30-d lifecycle in Meloidogyne arenaria as a means of tracking sporulation development. Detectable levels of transcripts of Spo0F were present as early as 5 d after the nematodes were exposes to Pasteuria spp. and were relatively constant throughout the 30-d lifecycle. Transcripts to Sigma-F were significantly higher in the middle of the lifecycle, while the transcripts of Sigma-G were detectable between 15 and 25 d, nearing the end of the lifecycle. These three markers can be used to track the process of sporulation in the nematode and augment microscopic observations. Tracking sporulation of Pasteuria spp. is important to fully realize its potential as a biological control method as it can more readily identify successful parasitism, define host ranges, and inform in vitro growth progress.
Maternal Stress Reduces the Susceptibility of Root-Knot Nematodes to Pasteuria Penetrans
Authors: Liu Chang , Ji Pingsheng , Timper Patricia 
Published: September 2019

Abstract

Pasteuria penetrans is an obligate parasite of root-knot nematodes (Meloidogyne spp.). Endospores of P. penetrans attach to the cuticle of second-stage juveniles (J2) and complete their life cycle within the nematode female body. Infected females will be filled with spores and will be sterilized. Studies with Daphnia magna and its parasite Pasteuria ramosa showed that a poor maternal environment can lead to offspring resistant to P. ramosa. Therefore, we hypothesized that Meloidogyne arenaria females raised under a stressed environment would produce offspring that were more resistant to P. penetrans. Females were exposed to a stressed environment created by crowding and low-food supply, or a non-stressed environment and their offspring evaluated for endospore attachment and infection by P. penetrans. No difference in spore attachment was observed between the two treatments. However, infection rate of P. penetrans in the stressed treatment was significantly lower than that in the non-stressed treatment (8 vs 18%). Mothers raised under stressed conditions appeared to produce more resistant offspring than did mothers raised under favorable conditions. Under stressful conditions, M. arenaria mothers may provide their progeny with enhanced survival traits. In the field, when nematode populations are not managed, they often reach the carrying capacity of their host plant by the end of the season. This study suggests that the next generation of inoculum may be more resistant to infection by P. penetrans.
Morpho-molecular characterization of Colombian and Brazilian populations of Rotylenchulus associated with Musa spp
Authors: Riascos-Ortiz Donald , Mosquera-Espinosa Ana Teresa , De Agudelo Francia Varón , de Oliveira Claudio Marcelo Gonçalves , Muñoz-Flórez Jaime Eduardo 
Published: September 2019

Abstract
Three populations, two from Colombia and one from Brazil, of Rotylenchulus reniformis associated with banana and plantain, were characterized using morphological, morphometric, and molecular methods. Morphometric data from these populations were similar to type and reference populations of R. reniformis. Partial sequences of both D2-D3 rDNA and mitochondrial cytochrome oxidase subunit I (COI) regions had a strong affinity (99% similarity) to previously published sequences of R. reniformis. Phylogenetic analyses (maximum likelihood and Bayesian inference) suggested that the Colombian populations of R. reniformis corresponded to the previously described Type A of the species. This is the definitive first report in Colombia of R. reniformis associated with banana and plantain crops.
Genome Announcement: The Draft Genomes of Two Radopholus similis populations from Costa Rica
Authors: Wram Catherine L. , Hesse Cedar N. , Wasala Sulochana K. , Howe Dana K. , Peetz Amy B. , Denver Dee R. , Humphreys-Pereira Danny , Zasada Inga A. 
Published: September 2019

Abstract

Radopholus similis is an economically important pest of both banana and citrus in tropical regions. Here we present draft genomes from two populations of R. similis from Costa Rica that were created and assembled using short read libraries from Illumina HiSeq technology.
Data of an Iranian Population of L. proximus Sturhan & Argo, 1983, with taxonomic revision of L. israelensis Peneva, Orion, Shlevin, Bar-Eyal & Brown, 1998 (Nematoda: Longidoridae) and Proposal for a New Synonymy
Authors: Giti Mazdosht , Kashi Leila , Pedram Majid 
Published: September 2019

Abstract
The morphology and morphometric characteristics of a Longidorus population recovered from a wheat-potato field in Hamadan province, western Iran, fit well with those given for two species, L. proximus and L. israelensis. The Iranian population was characterized by 5.6 to 8.6-mm-long females having a 17 to 21-µm-wide lip region separated from the rest of the body by a shallow depression, pocket-shaped amphidial fovea with a simple base and a ventral enlargement, a guiding ring at 31 to 40 µm distance from the anterior end, 108 to 127-µm-long odontostyle, 58 to 64-µm-long odontophore, 101 to 129-µm-long pharyngeal bulb with remarkably larger dorsal gland nucleus (at 49 to 53% of the bulb length) and two smaller ventrosublateral nuclei (at 66 to 76% of the pharyngeal bulb length), four juvenile developmental stages, and a rare male. The morphological and molecular data corroborated its assignment to the species L. proximus. In molecular phylogenetic analyses using partial LSU rDNA D2-D3 sequences, the presently studied Iranian population and previously sequenced isolates of L. proximus formed a clade with L. cretensis, L. iranicus, L. pseudoelongatus, and L. closelongatus, all except L. pseudoelongatus with no available data, having the similar pharyngeal gland nuclei size and arrangement. In internal transcribed spacer 1 (ITS1) phylogeny, it formed a clade with L. sturhani and four aforementioned species. The characters delimiting the two species L. proximus and L. israelensis were discussed and a new synonymy was proposed.
Identification of Suitable Meloidogyne spp. Housekeeping Genes
Authors: Hu Weiming , DiGennaro Peter M. 
Published: September 2019

Abstract
Gene expression studies often require reliable housekeeping (HK) genes to accurately capture gene expression levels under given conditions. This is especially true for root-knot nematodes (RKN, Meloidogyne spp.), whose drastic developmental changes are strongly dependent upon their environment. Here we utilized a publicly available M. hapla RNAseq database to identify putative HK genes throughout the nematode lifecycle. We then validated these candidate HK genes on M. incognita in order to develop a small library of suitable HK genes for RKN. Seven putative HK genes were selected for validation based on high expression level and ease of primer design. The expression of these genes was quantified by qPCR at different developmental stages to capture the entire life cycle of M. incognita which included eggs and naive infective juveniles through 3-wk post inoculation. Two algorithms, geNorm and Normfinder, identified three genes (Disu, Poly, and Skinase) constitutively and uniformly expressed throughout the entire life cycle of RKN. We believe these genes are superior HK genes suitable to be used as internal reference genes at all stages of RKN. Importantly, while we identified Actin, a commonly used HK gene, as a candidate gene within our RNAseq analyses, our qPCR results did not demonstrate stable expression throughout the nematode life cycle of this gene. This study successfully validated suitable HK genes utilizing both RNAseq data and standard qPCR methods across two species of RKN; suitable HK genes are likely applicable to other species of RKN, or even plant-parasitic nematodes. Additional lists of potential HK genes are also provided if the nematode of interest does not have homologues of the three superior reference genes described here. Gene expression studies on RKN should use validated HK genes to ensure accurate representation of transcript abundance.
Updated description of Paratylenchus lepidus Raski 1975 and P. minor Sharma, Sharma and Khan, 1986 by integrating molecular and ultra-structural observations
Authors: Maria Munawar , Miao Wentao , Ye Weimin , Zheng Jingwu 
Published: September 2019

Abstract
Two populations of Paratylenchus lepidus and P. minor were detected in the rhizosphere of Elaeocarpus sp. and Chinese red pine from Taizhou and Hangzhou, Zhejiang Province, China. Previously, P. lepidus has been reported from China whereas P. minor was originally described from India decades ago in the rhizosphere of peach but was never reported thereafter. In this study, both species were characterized morphologically and molecularly coupled with SEM observations. Morphologically, both species have four incisures in the lateral field, vulval present (SEM observations), stylet less than 30 μ m long and cephalic region without submedian lobes. Phylogenetically, both species grouped with paratylenchid species having short stylets. Both species can be differentiated from each other by the shape of lip region (rounded in P. lepidus and narrow truncated in P. minor) and tail terminus (pointed in P. lepidus and a broadly rounded in P. minor) and several morphomemtrical values.The study provided an updated description of P. lepidus and P. minor and a first record of P. minor from China. In addition, both species are the first paratylenchid species recorded from Elaeocarpus sp. and Pinus tabuliformis, respectively.
First report of Longidorus mindanaoensis Coomans, De Ley, Jimenez and De Ley, 2012 (Nematoda: Longidoridae) From a Mangrove Forest in Vietnam
Authors: Nguyen Thi Duyen , Nguyen Huu Tien , Linh Le Thi Mai , Nobleza Neriza , Trinh Quang Phap 
Published: September 2019

Abstract

Longidorus mindanaoensis was recovered from a mangrove forest in Vietnam. The recovered population is in general morphological agreement with the type population, and the characters of pharyngeal bulb, i.e. the same unique pattern of pharyngeal glands nuclei as well as the lip region morphology, amphidial fovea shape and size and position of vulva corroborated its identity. Molecular studies of the recovered population using D2–D3 expansion segments of large subunit ribosomal DNA (LSU rDNA D2–D3) revealed the D2–D3 sequence of recovered population is 99.6% similar to the sequence of the type population. A new morphometric range for body size was recorded for the species based upon present Vietnamese population, and present study emphasized the diversity of Longidorus spp. in Vietnam could be higher than previously assumed.
Percepciones sobre la influencia de los estilos de aprendizaje en el aprendizaje colaborativo en entornos virtuales
Authors: Nakova Katileva Eleonora , Romero Carbonell Marc 
Published: September 2019

Antecedentes: El presente artículo tiene como objetivo conocer las percepciones de los estudiantes en línea sobre la colaboración en contextos virtuales, cuáles son los estilos de aprendizaje predominantes entre los participantes y cómo condicionan el proceso colaborativo. Metodología: Se contactó con 215 participantes configurando una muestra de tipo no probabilístico. Es un estudio de caso basado en los estudiantes de la UOC que aplica la metodología mixta obteniendo datos a través de cuestionarios y entrevista. Resultados: Los hallazgos señalaron que el proceso de aprendizaje colaborativo es aún insatisfactorio para gran parte de la muestra, el 47 % de la cual comparte el estilo de aprendizaje activo. Discusión y conclusiones: En el artículo se ha argumentado la necesidad de mejorar el proceso de aprendizaje colaborativo en línea y se ha sugerido formar los grupos de teniendo en cuenta los estilos de aprendizaje de los participantes.
In Vitro Antitumor Evaluation of Some Hybrid Molecules Containing Coumarin and Quinolinone Moieties
Authors: Rizzk Youstina W. , El-Deen Ibrahim M. , Mohammed Faten Z. , Abdelhamid Moustafa S. , Khedr Amgad I.M. 
Published: September 2019

Background:Hybrid molecules furnished by merging two or more pharmacophores is an emerging concept in the field of medicinal chemistry and drug discovery. Currently, coumarin hybrids have attracted the keen attention of researchers to discover their therapeutic capability against cancer.Objective:The present study aimed to evaluate the in vitro antitumor activity of a new series of hybrid molecules containing coumarin and quinolinone moieties 4 and 5 against four cancer cell lines.Materials and Methods:A new series of hybrid molecules containing coumarin and quinolinone moieties, 4a-c and 5a-c, were synthesized and screened for their cytotoxicity against prostate PC-3, breast MCF-7, colon HCT- 116 and liver HepG2 cancer cell lines as well as normal breast Hs-371 T.Results:All the synthesized compounds were assessed for their in vitro antiproliferative activity against four cancer cell lines and several compounds were found to be active. Further in vitro cell cycle study of compounds 4a and 5a revealed MCF-7 cells arrest at G2 /M phase of the cell cycle profile and induction apoptosis at pre-G1 phase. The apoptosis-inducing activity was evidenced by up-regulation of Bax protein together with the downregulation of the expression of Bcl-2 protein. The mechanism of cytotoxic activity of compounds 4a and 5a correlated to its topoisomerase II inhibitory activity.Conclusion:Hybrid molecules containing coumarin and quinolinone moieties represents a scaffold for further optimization to obtain promising anticancer agents.
A Biofeedback App to Instruct Abdominal Breathing (Breathing-Mentor): Pilot Experiment
Authors: Faust-Christmann Corinna Anna , Taetz Bertram , Zolynski Gregor , Zimmermann Tobias , Bleser Gabriele 
Published: September 2019

Background
Deep and slow abdominal breathing is an important skill for the management of stress and pain. However, despite multiple proofs on the effectiveness of biofeedback, most breathing apps remain limited to pacing specific breathing patterns, without sensor feedback on the actual breathing behavior.

Objective
To fill this gap, an app named Breathing-Mentor was developed. This app combines effective visualization of the instruction with biofeedback on deep abdominal breathing, based on the mobile phone’s accelerometers. The aim of this pilot study was to investigate users’ feedback and breathing behavior during initial contact with the app.

Methods
To reveal the possible effects of biofeedback, two versions of the mobile app were developed. Both contained the same visual instruction, but only the full version included additional biofeedback. In total, 40 untrained participants were randomly assigned to one of the two versions of the app. They had to follow the app’s instructions as closely as possible for 5 min.

Results
The group with additional biofeedback showed an increased signal-to-noise ratio for instructed breathing frequency (0.1 Hz) compared with those using visual instruction without biofeedback (F1,37=4.18; P<.048). During this initial contact with the full version, self-reported relaxation effectivity was, however, lower than the group using visual instruction without biofeedback (t37=−2.36; P=.02), probably owing to increased cognitive workload to follow the instruction.

Conclusions
This study supports the feasibility and usefulness of incorporating biofeedback in the Breathing-Mentor app to train abdominal breathing. Immediate effects on relaxation levels should, however, not be expected for untrained users.
O Pluralismo De Sentidos Da Cultura Da Mídia Contemporânea
Authors: Reis João Montenegro 
Published: September 2019

O artigo a ser apresentado consiste na análise dos filmes “O Senhor das Armas”; “Syriana” e “Diamante de Sangue, ”três grandes produções dos estúdios de Hollywood. Tratam-se de produtos culturais da mídia, que estão francamente inseridos na lógica do mercado, mas cujo conteúdo, apesar do caráter apelativo, apresenta um poder de significação diversificado. Com base nos estudos culturais britânicos e nas análises multi-perspectívicas de Douglas Kellner, que utiliza recursos históricos e das teorias social e da comunicação, este trabalho propõe uma visão diferente quanto aos produtos culturais de massa contemporâneos. O artigo busca provar que há valores, significados e mensagens produzidos pela cultura da mídia, que induzem o telespectador à reflexão crítica e não apenas ao puro entretenimento e à conformidade social.
"Ao povo rio-grandense entrego as minhas provincianas": a literatura no século XIX e a obra de Bernardo Taveira Junior
Authors: Gonçalves Mariana Couto 
Published: September 2019

Durante a segunda metade do século XIX, a Província do Rio Grande do Sul viu despertar em seu solo uma gama de jornais de caráter exclusivamente literário que tinham como objetivo promover os autores locais e regionais. Dessa forma, o artigo visa apresentar os primeiros periódicos literários, destacando a obra do escritor Bernardo Taveira Junior, que publicou inúmeros poemas, romances-folhetins, crônicas e críticas nestes periódicos e que teve um papel fundamental como colaborador e propagador dos ideais defendidos por estes jornais. Além disso, o artigo propõe analisar a obra “Provincianas” onde Bernardo Taveira Junior apresenta os costumes e tradições do Rio Grande do Sul através de dezoito poemas.
Paisagem sonora do boi do Norte: a música que cura todos os males
Authors: Furlanetto Beatriz Helena , Kozel Salete 
Published: September 2019

O texto discute o conceito de paisagem sonora na perspectiva da geografia cultural. Ao contemplar a dimensão material e simbólica da existência humana, a paisagem reflete a identidade dos grupos culturais e emerge segundo as experiências de cada indivíduo. Para Berque, a paisagem é marca e matriz da cultura, expressa uma civilização e transmite usos e significações de uma geração à outra. Cosgrove considera a paisagem como um texto cultural, que apresenta a possibilidade de múltiplas leituras apreendidas através de poesias, letras de músicas, filmes, pinturas e outras representações. A paisagem sonora é o ambiente sonoro da humanidade, um conjunto sempre presente de sons que estão constantemente em mutação, de acordo com Schafer. Nesse sentido, é possível investigar a paisagem sonora do boi-de-mamão, considerando-o uma criação coletiva capaz de revelar os sentidos que determinados sujeitos atribuem ao seu ambiente. A pesquisa tem como orientação a abordagem fenomenológica, fundamentada na análise documental, bibliográfica e pesquisa de campo. O boi-de-mamão, denominação atribuída ao bumba-meu-boi nos Estados do Paraná e Santa Catarina, é um folguedo brasileiro resultante da união de elementos das culturas europeia, indígena e africana, na qual o boi é a principal figura de representação. Com o objetivo de apreender os sentidos que os participantes do folguedo atribuem ao seu espaço, realizou-se a análise parcial de uma entrevista com a vice-presidente do Boi do Norte, um grupo de boi-de-mamão da cidade de Antonina, no litoral paranaense. São apontadas algumas reflexões que emergem da fala da entrevistada, como questões relacionadas à sociabilidade, à espacialidade e à música. Considerando a fase inicial dessa pesquisa, os resultados são parciais, mas evidenciam a riqueza do objeto e a urgência de maiores investigações.
O CURRÍCULO GEOGRÁFICO DOS ANOS INICIAIS: DA SUA HISTÓRIA ÀS SUAS DIRETRIZES
Authors: Gorziza Henrique Silva 
Published: September 2019

O trabalho introduz e caracteriza o ensino dos conteúdos geográficos nos Anos Iniciais do Ensino Fundamental. Apresenta um breve histórico da Geografia trabalhada nos currículos de acordo com a Lei de Diretrizes e Bases que instituiu os documentos oficiais do país. Objetiva desconstruir algumas particularidades existentes nesses documentos, visando também entender o currículo através de uma análise teórica sobre o seu papel desempenhado no ensino da geografia escolar. Por meio da análise realizada, foi possível compreender lacunas que caracterizam um desencontro nos apontamentos presentes nas suas diretrizes e orientações, as quais nos levam a refletir sobre o currículo existente nas rotinas escolares. Foi possível evidenciar a importância do poder do currículo escolar na construção de saberes e valores.
NEM SÓ DE MAPAS SE FAZ A GEOGRAFIA: os diferentes nas aulas de Geo
Authors: Cidade Borges Rubia Aparecida 
Published: September 2019

Esse trabalho visa publicizar algumas discussões, realizadas em Dissertação de Mestrado, sobre alternativas às práticas homogeneizadoras de identidades na escola, de modo a, através das aulas de geografia, desnaturalizar discursos sobre as minorias e/ou grupos socialmente desfavorecidos (nessa escrita chamados de diferentes: mulheres, público LGBT, negros e refugiados), contribuindo para superar a subjetividade contrária à promoção dos Direitos Humanos e oportunizando a desconstrução de estereótipos presentes no "senso comum". Para tal, os ditos dos/as alunos/as de 9.º ano de escola pública municipal foram coletados, no ano letivo de 2016, e foram analisados com o apoio da bibliografia selecionada, a partir dos pressupostos dos Estudos Culturais e do ensino contemporâneo de Geografia Escolar.
Tectônicas Digitais: a (in) tangibilidade no processo de projeto em arquitetura
Authors: De Almeida Caio Augusto Rabite , Lima Fernando Tadeu de Araújo , Borges Marcos Martins 
Published: September 2019

Esse artigo é resultado de uma pesquisa de Mestrado que centrou-se na investigação da relação entre o estudo das tectônicas em paralelo com a evolução das técnicas e conhecimentos envolvidos no processo de projeto em arquitetura. O conceito de Tectônica em Arquitetura é uma maneira de se falar sobre o ambiente construído em que o seu uso, estrutura e experiência estão explicitamente relacionados à materialização e à construção deste ambiente construído. Nos últimos anos as novas tecnologias digitais e os processos de produção pós-industriais surgiram como ferramentas de apoio que modificam a maneira de se projetar, avaliar e produzir este ambiente construído, incorporando-se assim uma lógica digital ao conceito tradicional de tectônicas. Como resultado, este trabalho é direcionado no emprego de softwares computacionais paramétricos para a proposição de um experimento intitulado Pavilhão Waffle, que foi realizado com a combinação de variáveis e parâmetros digitais e construtivos, objetivando perceber o potencial em que cada um desses diversos níveis se combinam, em uma série de campos de estudo e em um cruzamento multi escalar de arquitetura, engenharia, computação, análises físicas e experimentações de design e construção.
Palavras-chave: Tectônicas Digitais; Processo de Projeto; Arquitetura Paramétrica; Fabricação Digital.