bluuebunny/crossref_metadata_2025_split huggingface dataset:
Extracellular vesicles as a novel therapeutic tool for cell‐free regenerative medicine in oral rehabilitation
Authors: Lv Longwei , Sheng Chunhui , Zhou Yongsheng 
Published: September 2019

AbstractOral maxillofacial defects may always lead to complicated hard and soft tissue loss, including bone, nerve, blood vessels, teeth and skin, which are difficult to restore and severely influence the life quality of patients. Extracellular vesicles (EVs), including exosomes, microvesicles and apoptotic bodies, are emerging as potential solutions for complex tissue regeneration through cell‐free therapies. In this review, we highlight the functional roles of EVs in the regenerative medicine for oral maxillofacial rehabilitation, specifically bone, skin, blood vessels, peripheral nerve and tooth‐related tissue regeneration. Publications were reviewed by two researchers independently basing on three databases (PubMed, MEDLINE and Web of Science), until 31 December 2018. Basing on current researches, we classified the origin of EVs for regenerative medicine into four categories: related cells in the regenerative niche, mesenchymal stem cells, immune cells and body fluids. The secretome of different cells are distinct, while the same cells secrete different EVs under varied conditions; therefore, the content profiles of EVs and regulatory mechanisms on target cells are compared and emphasised. By unravelling the regulatory mechanisms of EVs in tissue regeneration, modified cells and tailored EVs with specific target may be produced for precision medicine with high efficacy.
Temporomandibular disorders, pain in the neck and shoulder area, and headache among musicians
Authors: van Selms Maurits K. A. , Wiegers Jetske W. , van der Meer Hedwig A. , Ahlberg Jari , Lobbezoo Frank , Visscher Corine M. 
Published: September 2019

AbstractBackgroundUncertainties still exist about the role of playing musical instruments on the report of musculoskeletal complaints and headache.ObjectivesTo evaluate the prevalence of and risk indicators for symptoms of temporomandibular disorders, pain in the neck or shoulder, and headache among musicians.MethodsA questionnaire was distributed among 50 Dutch music ensembles.ResultsThe questionnaire was completed by 1470 musicians (response rate 77.0%). Of these, 371 musicians were categorised as woodwind players, 300 as brass players, 276 as upper strings players, 306 as vocalists and 208 as controls; nine musicians had not noted their main instrument. The mean age was 41.6 years (standard deviation [SD] 17.2), and 46.5% were male. Irrespective of instrumentalist group, 18.3% of the musicians reported TMD pain, 52.5% reported pain in the neck and shoulder area, and 42.5% reported headache. Of the functional complaints, 18.3% of the musicians reported TMJ sounds, whereas a jaw lock or catch on opening or on closing was reported by 7.1% and 2.4%, respectively. TMD pain was associated with playing a woodwind instrument, whereas pain in the neck and shoulder was associated with playing the violin or viola. For each complaint, oral behaviours were found as risk indicator, supplemented by specific risk indicators for the various complaints.ConclusionsThe current finding that pain‐related symptoms varied widely between instrumentalist groups seems to reflect the impact of different instrument playing techniques. Playing a musical instrument appears not the primary aetiologic factor in precipitating a functional temporomandibular joint problem.
Development of an Oral and Maxillofacial Frailty Index: A preliminary study
Authors: Choi Jee‐Hye , Kang Jeong‐Hyun , Koh Sang‐Baek , Kim Nam‐Hee , Kho Hong‐Seop 
Published: September 2019

AbstractObjectiveThe purpose of this study is to develop an index to measure oral and maxillofacial frailty in older adults as an acceptable screening tool.MethodsThree hundred adults (aged ≥ 50 years) participated in this study by telephone interview. Ten candidate items for the Oral and Maxillofacial Frailty Index (OMFI) and 20 matched detailed items for oral and maxillofacial functions were asked. Information about sociodemographic and oral health‐related variables was also obtained. The test‐retest reliability of the 10 candidate items was determined. Correlations of the 10 candidate items with the 20 detailed items or sociodemographic and oral health‐related variables were analysed to test the validity of the candidate items. To suggest optimal items for the OMFI, five different models with different numbers of items were constructed and evaluated based on their associations with main variables.ResultsThe overall internal consistency of the 10 candidate items was .704, and the Cronbach's alpha value of each item was .23‐.55. The test‐retest reliability was excellent. There were significant correlations between the 10 candidate and 20 matched detailed items. The score of the 10 candidate items was significantly different according to gender, education level and oral health‐related variables. The final five items for the OMFI were difficulties in chewing, the necessity of water when eating dry food, difficulties in jaw or tongue movements, difficulties in speaking or pronunciation and difficulties in facial expression.ConclusionsThe five items could be used as a screening tool for evaluating oral and maxillofacial frailty in older adults.
Frequent delayed spontaneous seroclearance of hepatitis B virus after incident HBV infection among adult high‐risk groups
Authors: van Santen Daniela K. , Boyd Anders , Bruisten Sylvia , Sonder Gerard JB , Prins Maria , van Houdt Robin 
Published: September 2019

AbstractHigh rates (~25%) of developing chronic hepatitis B virus (HBV) infection (hepatitis B surface antigen (HBsAg)‐positive for > 6 months following infection) have been observed in people who use drugs (PWUD) and men who have sex with men (MSM). We aimed to estimate the frequency of delayed HBsAg seroclearance, along with its determinants, and time to delayed HBsAg seroclearance. Data were used from MSM and PWUD enrolled in the Amsterdam Cohort Studies (1985‐2002) who had anti‐hepatitis B core antibody seroconversion. Potential determinants for standard HBsAg seroclearance, delayed HBsAg seroclearance and chronic HBV were examined using multinominal logistic regression. Time to HBsAg seroclearance was estimated using Kaplan‐Meier curves. A total of 147 incident HBV infections occurred during follow‐up. On initial HBsAg testing after infection (6‐12 months), 42 (29%) were HBsAg‐positive and 105 (71%) were HBsAg‐negative (‘standard HBsAg seroclearance’). Of the 42 initially HBsAg‐positive individuals, 22 subsequently tested HBsAg‐negative (of whom 7 (31.8%) were HBV DNA positive at last visit, suggesting occult HBV). Overall, 15 became HBsAg‐negative and HBV DNA‐negative (‘delayed HBsAg seroclearance’), while 27 remained HBsAg and/or HBV DNA‐positive (‘chronic HBV’). The 5‐year cumulative probability of delayed HBsAg seroclearance was 41.6% for initially HBsAg‐positive individuals. Delayed HBsAg seroclearance and remaining chronically infected were associated with younger age and HIV/hepatitis C virus (HCV)‐co‐infection. In conclusion, delayed HBsAg seroclearance is common in these key adult populations at‐risk for HBV, while proportion developing HBV chronicity (18%) is still higher compared to the general population (~5%). Given the proportion of individuals with occult HBV infection and that HCV direct‐acting antivirals can lead to HBV reactivation, HBV DNA testing in HCV co‐infected MSM/PWUD are warranted prior to treatment initiation.
p38 mitogen‐activated protein kinase impairment of innate immune cells is a characteristic feature of HBeAg‐negative chronic hepatitis B
Authors: Bakarozi Marianna , Mavropoulos Athanasios , Bogdanos Dimitrios P. , Dalekos George N. , Rigopoulou Eirini I. 
Published: September 2019

AbstractThe mitogen‐activated protein kinase p38 (MAPK) is implicated in the induction of immune responses by regulating the differentiation of T lymphocytes and production of cytokines. Our aim was to investigate p38MAPK phosphorylation in different stages of the natural history of hepatitis B virus (HBV) infection. Peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll‐Hypaque density‐based centrifugation from 10 patients with HBeAg‐negative chronic hepatitis B [HBeAg(−) CHB;HBV‐DNA>2000IU/mL], eight patients with HBeAg‐negative chronic HBV infection [HBeAg(−) CI;undetectable HBV‐DNA] and 8 healthy controls (HCs). p38MAPK phosphorylation was assessed by phospho‐specific flow cytometry in PBMCs and cell subsets (CD3+,CD3−,CD56+,CD56−) after stimulation with cytokines (IL‐12+IL‐2 and IL‐12+IL‐18) or nonspecific stimuli [arsenite, phorbol 12‐myristate 13‐acetate (PMA) and ionomycin] at 0,30,60,120 and 240 minutes using p38 phospho‐specific conjugated antibodies. ΙFN‐γ was determined by ELISA in PBMCs culture supernatants after stimulation with rhIL‐2, rhIL‐12 and rhIL‐18, with and without pre‐treatment with the p38 MAPK inhibitor, SB203580. HBeAg(−) CI patients showed the highest expression of phosphor‐p38 MAPK in total PBMCs and subpopulations compared to HBeAg(−) CHB and HCs. A striking impairment in p38 phosphorylation was noted in CD56+ cells and in especially in NK cells (CD3‐CD56+). SB203580‐induced inhibition of p38MAPK phosphorylation was associated with suppression of IFN‐γ production in all groups. The universal lack of p38 MAPK activation in CD56+ and in particular in NK cells from HBeAg(−) CHB patients during viremia suggests a potential cell‐dependent implication of this pathway in the natural history of HBV infection.
Alloparental care in the sea: Brood parasitism and adoption within and between two species of coral reef Altrichthys damselfish?
Authors: Tariel Juliette , Longo Gary , Quiros Angela , Crane Nicole L. , Tenggardjaja Kimberly , Jackson Alexis , Lyon Bruce E. , Bernardi Giacomo 
Published: September 2019

AbstractThe evolution of parental care opens the door for the evolution of brood parasitic strategies that allow individuals to gain the benefits of parental care without paying the costs. Here we provide the first documentation for alloparental care in coral reef fish and we discuss why these patterns may reflect conspecific and interspecific brood parasitism. Species‐specific barcodes revealed the existence of low levels (3.5% of all offspring) of mixed interspecific broods, mostly juvenile Amblyglyphidodon batunai and Pomacentrus smithi damselfish in Altrichthys broods. A separate analysis of conspecific parentage based on microsatellite markers revealed that mixed parentage broods are common in both species, and the genetic patterns are consistent with two different modes of conspecific brood parasitism, although further studies are required to determine the specific mechanisms responsible for these mixed parentage broods. While many broods had offspring from multiple parasites, in many cases a given brood contained only a single foreign offspring, perhaps a consequence of the movement of lone juveniles between nests. In other cases, broods contained large numbers of putative parasitic offspring from the same parents and we propose that these are more likely to be cases where parasitic adults laid a large number of eggs in the host nest than the result of movements of large numbers of offspring from a single brood after hatching. The evidence that these genetic patterns reflect adaptive brood parasitism, as well as possible costs and benefits of parasitism to hosts and parasites, are discussed.
Tumor depth of invasion and prognosis of early‐stage oral squamous cell carcinoma: A meta‐analysis
Authors: Caldeira Patrícia Carlos , Soto Andrea María López , de Aguiar Maria Cássia Ferreira , Martins Carolina Castro 
Published: September 2019

AbstractObjectivesTo assess the prognosis for early‐stage oral squamous cell carcinoma according to tumor depth of invasion (DOI).MethodsThis study was logged in the PROSPERO database under protocol # CRD42017059976. The search was conducted in six electronic databases up to May 2019. Fixed‐effects meta‐analysis was performed for the calculation of the odds ratio (OR) and respective 95% CI. Primary outcomes were lymph node metastasis, recurrence, and survival. Heterogeneity was calculated by the I2 test. The certainty of evidence was assessed by Grading of Recommendations, Assessment, Development, and Evaluation (GRADE) approach.ResultsTwenty‐seven studies were included (19 in the meta‐analysis) with 2,404 patients with a mean of 60 years of age. High tumor DOI is associated with a greater chance of presenting lymph node metastasis, regardless of the cutoff point for DOI (13 meta‐analysis; OR 1.69–53.08), recurrence (five meta‐analysis; OR 1.22–3.83), and lower chance of survival (1 meta‐analysis; OR 0.49). The certainty of evidence varied from very low to low.ConclusionsTumor DOI is a good prognosticator for early‐stage OSCC. The findings of the current meta‐analysis highlight the clinical relevance of DOI and corroborate its incorporation for staging OSCC.
Type I interferon and interferon‐stimulated gene expression in oral epithelial cells
Authors: Brice D.C. , Figgins E. , Yu F. , Diamond G. 
Published: September 2019

AbstractOral epithelial cells (OEC) represent the first site of host interaction with viruses that infect the body through the oral route; however, their innate antiviral defense mechanisms yet to be defined. Previous studies have determined that OEC express pathogen‐, damage‐, or danger‐associated molecular patterns (PAMPs or DAMPs), but their expression of key antiviral innate immune mediators, including type I interferons (type I IFN) and interferon‐stimulated genes (ISGs) has not been studied extensively. We used the oral keratinocyte cell line, OKF6/TERT1, in the presence and absence of the viral mimics poly(I:C) and unmethylated CpG DNA, to define the expression of type I IFN and ISGs. We identified the basal expression of novel type I IFN genes IFNE and IFNK, while IFNB1 was induced by viral mimics, through the nuclear translocation of IRF3. Numerous ISGs were expressed at basal levels in OEC, with an apparent correlation between high expression and antiviral activity at the earlier stages of viral infection. Stimulation of OECs with poly(I:C) led to selective induction of ISGs, including MX1, BST2, PML, RSAD2, ISG15, and ZC3HAV1. Together, our results demonstrate that OECs exhibit a robust innate antiviral immune defense profile, which is primed to address a wide variety of pathogenic viruses that are transmitted orally.
Risk factors for unsuccessful atropine therapy in hypertrophic pyloric stenosis
Authors: Ono Sachie , Takenouchi Ayako , Terui Keita , Yoshida Hideo , Terui Elena 
Published: September 2019

AbstractBackgroundI.v. atropine (IA) for infantile hypertrophic pyloric stenosis (IHPS) is a good alternative to pyloromyotomy but has not been broadly accepted. The lower success rate is one of the greatest disadvantages of IA. We investigated the risk factors for unsuccessful results following IA for IHPS.MethodsMedical records of patients with IHPS who were admitted to Kimitsu Chuo Hospital between 2002 and 2016 and were initially given atropine sulfate were retrospectively reviewed. Atropine was given i.v. (0.1 mg/kg/day in eight divided doses). Oral feeding of milk was started with a small amount and increased in a stepwise fashion to full feed. IA therapy was defined as unsuccessful in the presence of projectile vomiting more than three times a day or intolerance to a predetermined amount of milk.ResultsOf the 48 patients with IHPS, 33 patients were successfully treated with IA and 15 patients needed surgical intervention. On univariate analysis the risk factors for unsuccessful IA therapy were younger age, lower bodyweight, and shorter duration of symptoms before diagnosis. On multivariate analysis age at diagnosis < 30 days was the only significant risk factor for unsuccessful IA therapy (OR, 5.7 l P = 0.03).ConclusionsAge at diagnosis < 30 days is a risk factor for unsuccessful IA therapy in IHPS. This might be considered when IA therapy is used for neonates with IHPS.
A comparative clinical trial to evaluate efficacy and safety of the 308‐nm excimer laser and the gain‐switched 311‐nm titanium:sapphire laser in the treatment of vitiligo
Authors: Park Myeong Jin , Shon Uri , Seong Gi Hyun , Kim Myung Hwa , Park Byung Cheol , Hong Seung Phil 
Published: September 2019

AbstractBackground/purposeUltraviolet B (UVB) laser irradiation in a targeted manner is a reasonable treatment option for localized vitiligo. Recently, narrow‐band UVB gain‐switched 311‐nm titanium:sapphire lasers (TSL) were developed for the treatment of localized vitiligo. We aimed to compare efficacy, patient satisfaction, and safety between the conventional 308‐nm excimer laser (EL) and gain‐switched 311‐nm TSL in patients with vitiligo.MethodsThe 13‐paired lesions from 10 patients who had small vitiligo patches were included in this prospective intra‐patient comparison trial. Each pair was randomly assigned to each laser treatment group and treated twice weekly for 12 weeks. The global photographic assessments by dermatologists, objective numerical assessments by imaging analyzer, and patient's satisfaction were used to evaluate the effectiveness. Adverse effects were also investigated at every visit.ResultsAll treated lesions showed improvement of about 50% after 12 weeks. There was no significant difference between EL‐ and TSL‐treated groups. Patient satisfaction and preference among the groups were also similar. Regarding safety, there were no serious adverse effects requiring cessation of the treatments; however, the severity score for persistent erythema (lasting >24 hours) was significantly lower in the TSL group than in the EL group.ConclusionsThe gain‐switched 311‐nm TSL exhibited similar efficacy to the 308‐nm EL in treating vitiligo as well as improved safety. Therefore, the 311‐nm TSL is considered as a candidate device to replace the EL as a new and promising treatment option for localized vitiligo.
Pollination and resource limitation as interacting constraints on almond fruit set
Authors: Cunningham S. A. , Evans M. J. , Neave M. , Armstrong J. , Barton P. S. 
Published: September 2019

Abstract

Pollination and resource availability are factors determining reproductive success of plants, and in agriculture these factors influence yield of fruit‐bearing crops. Our understanding of the importance of crop pollination is fast improving, but less is known about how the interaction between pollination and resources constrains fruit production.

We conducted an experiment with almond trees (Prunus dulcis) to examine how the number of flowers, light availability and competition for resources affected nut (fruit) production on individual spurs (fruit‐bearing structures) exposed to open‐pollination or hand‐pollination.

We found a positive relationship between flower number and nut number on spurs with up to four flowers, but no further benefit after four flowers, suggesting a resource threshold expressed by individual spurs. Spurs with few flowers increased the conversion rate of flowers to nuts when supplemented with hand‐pollination, but spurs with more flowers were more likely to achieve the threshold number of nuts even under open‐pollination. Our experiment included a further treatment involving spraying whole trees with pollen. This treatment reduced nut production by spurs with many flowers and high light availability, suggesting competition is experienced by well‐resourced spurs when resources need to be shared among developing nuts across the whole tree.

Our study supports the hypothesis that excess flower production in fruit trees increases the potential for fruit production when pollinator and resource availability is variable (bet‐hedging). Spurs with more flowers typically produce more nuts (within a limited range), but only if both resources and pollen supply increase with flower number. For almond growers, a focus on maintaining high flower numbers, especially in high light regions of the canopy, is the foundation for high levels of production. Strategies to lift flower number and light are complicated by trade‐offs inherent in tree architecture and orchard design. However, fruit set would be lifted above that achieved by current practice by an increase in the pollination rate of flowers.
The preliminary studies on protein profile in retained and not retained foetal membranes in heavy draft mares
Authors: Wawrzykowski Jacek , Rapacz‐Leonard Anna , Wiącek Dariusz , Kankofer Marta , Janowski Tomasz 
Published: September 2019

AbstractProtein profile of the placenta expresses its function and maintenance. Any alterations can be reflected in qualitative and quantitative changes in this profile. The aim of the present study was the evaluation of protein profile in the placenta of mares suffering from the retention of foetal membranes (FMR) by two separation methods and the comparison with physiologically released tissues. Placentas from 14 healthy, heavy draft mares were collected immediately after the expulsion of newborn. Tissues after homogenization and staining with fluorescent dyes were subjected to electrophoretic as well as chromatographic separation. Electrophoretic gels were statistically analysed for the presence and abundance of examined proteins, while some proteins were identified in chromatographic fractions. Out of 248 spots detected in endometrium, 38 differed significantly between FMR and control animals, while in allantochorion, respective values reached 241 and 27 spots (p < .05). Among identified proteins that expressed higher abundance in endometrium of FMR mares than control animals were prostaglandin reductase, dehydrogenase/reductase SDR family, and placental growth factor. These proteins are involved in regulation of parturition. Additionally, the following proteins responsible for physiological activity of a cell—guanine methyl transferase, aspartyl/asparaginyl beta‐hydroxylase and GTP‐binding protein, were identified. These proteins expressed higher abundance in allantochorion of FMR mares than in controls. This preliminary study confirmed the disturbances in protein pattern between foetal membranes in FMR and healthy mares. Further qualitative and quantitative experiments are necessary to deepen our knowledge on the mechanisms of the retention of foetal membranes in mares.
A genetically informed examination of the relations between inaccurate emotion expression and recognition and experiencing peer victimization
Authors: DiLalla Lisabeth Fisher , John Sufna Gheyara 
Published: September 2019

AbstractThis study examines children's abilities to accurately portray emotions (emotion expression; EE) and to read others' emotions (emotion recognition; ER) as possible genetically influenced behaviors that may increase vulnerability to victimization. In this study of 127 6–10‐year‐old multiples, children were assessed for EE accuracy by being photographed when told to display different emotions; photographs were subsequently rated for emotion accuracy. Children also were assessed for ER accuracy on a computer task by rating the emotions of displayed children's faces. Genetic likelihood scores for angry and fearful EE and ER errors were calculated. Children also completed a victimization questionnaire. Results showed that children who were poor at making angry faces (EE angry misses) were less likely to be victimized, and children who were more likely to rate faces as fearful (ER fearful biases) were more likely to be victimized. ER fearful biases were related to victimization through a shared genetic link. Finally, demonstrating gene–environment correlation, girls with a genetic likelihood for not looking fearful when they were intending to (EE fearful misses) were significantly less likely to be victimized by peers. These results show that emotion skills surrounding expressing and recognizing anger and fear are associated with peer victimization risk.