bluuebunny/crossref_metadata_2025_split huggingface dataset:
Isolation and Characterization of Azotobacter from Neems Rhizosphere
Authors: Hala Yusminah , Ali Alimuddin 
Published: June 2019

Abstract
Nitrogen-fixing bacteria can be isolated from rhizosphere plants, and one of the N2-fixing bacteria is Azotobacter. One of the plants that can grow well in dry and barren soil conditions is Neem (Azadirachta indica A. Juss)(Mimba). The purpose of this study was to determined any Azotobacter isolated from the rhizosphere of the neem plant. The type of research is an experimental study, tracing the diversity of Azotobacter in the rhizosphere of the neem plant. The study was conducted by taking soil samples from the rhizosphere of neem plants on the Bali Island. Morphological characterization based on colony observation in terms of shape, color, margin, and texture. The isolated cells were determined based on Gram staining and determination of the presence of spores. Isolates were characterized by various biochemical methods namely nitrate, reductase, citrate utilization, methyl red, VP test, catalase (method of slip-cover), and oxidase tests. The soil sample was taken from rhizospheres of 5 plants. The results showed that the N2-fixing bacteria isolated in the five soil samples were Azotobacter paspali and Azotobacter vinelandii (Azomonas) groups. Actinomycetes were found when isolating using specific media Azotobacter on plant 2.
Development of Green Banana Cake (Pisang ijo) Products using Kelor Leaf (Moringa oleifera Lam) as a Food Dye and Flour Substitute
Authors: Nahriana  , Yahya Muhammad , Tawani Ratnawati 
Published: June 2019

Abstract
Pisang ijo is one of the traditional cakes in South Sulawesi which is very popular within the community. Pisang ijo is made from Moringa oleifera leaf flour and extract, wheat flour, plantain, sugar, and salt which is processed using steaming methods. Pisang ijo was served with vanilla sauce. The purpose of the study was to describe the process of making moringa leaf flour and extract, knowing how to process it and find out the results of organoleptic tests of green banana cake by using the substitution of moringa leaf extract (F1) and moringa leaf flour (F2). The research method used was an organoleptic test method using 30 investigators divided into three groups: 10 expert examiners, 10 trained investigators, and 10 untrained investigators. Laboratory tests using spectrophotometric were carried out to determine the nutritional content of moringa leaf flour and extract. The organoleptic test results show that from the aspect of color, the most preferred product by the panelists is the F1 product which produces a lighter green color. The F2 product is less bright. The hedonic test results of aroma, texture, and sense show that the product most preferred by the panelists is the F2 product. Proximate test results indicate a better nutritional content in F2 products, i.e., the protein and vitamin C content are higher, which are 3.38% and 153.2 μg g−1, respectively than the F1 product.
ICT Strategy and Micro-Entrepreneurs Growth: The Indonesian Case
Authors: Anwar Misita , Djawad Yasser A. , Ridwansyah  
Published: June 2019

Abstract
The importance of Information and Communication Technology has been recognized but often undervalued by small businesses. Furthermore, best practices of ICT use in large companies do not always translate to a small business environment. Few studies have been done at the intersection of ICT and Micro and Small Enterprises (MSEs) in a ‘holistic’ manner taking into consideration various aspects of ICT adoption and use. The LIAISE framework provides guidelines for investigating various aspects of ICT adoption and use, including ICT literacy, information, and content, access, infrastructure and support, and evaluation. The framework was originally developed for the non-profit sector and has been applied in the small business context by examining MSEs in developing countries. Using a case study in Indonesia, this paper will use LIAISE as an analytical framework to investigate mobile phone adoption and use by micro-entrepreneurs.
Microbial Augmentation on Intensive Grow Out Shrimp Culture to Improve Water Quality, Growth and Shrimp Production
Authors: Junda Muhammad , Pagarra Halifah , Nur Surachman 
Published: June 2019

Abstract
The objective of this study is to evaluate the microbial augmentation on intensive shrimp culture to improve water quality, growth, and shrimp productivity. This study was conducted on intensive shrimp grow out ponds of the farmers in Pangkep Regency, South Sulawesi. There were four grow out ponds used for study with different pond size. All ponds used zero or minimal water exchange systems. All inputs used in this study such as high quality of commercial feed, seed, intensive aeration with paddle wheels, vitamins and minerals. Treatment with a consortium of flock forming bacteria as a starter each given every week. Shrimp growth ponds used intensive culture system with condition water depth of ponds 120 – 130 cm, feeding rate was four time a day. Water quality parameters measured are salinity, pH, water temperature, dissolved oxygen (DO) and turbidity. Survival rate (SR), average daily gain (ADG), feed conversion ratio (FCR), and MBW were measured every week. The results of this research showed that shrimp production 1,600 – 5,600 kg. Growth rate: ADG 0.34 – 0.45; survival rate 90 – 97 %; FCR 1.02 – 1.1 and MBW 22.8 – 29.40. Water quality parameters such as dissolved oxygen, water temperature, water depth, salinity, pH and water transparency were 2.8 – 5.5 mg L−1; 26 – 31°C; 75 – 125 cm; 15-30 mg L−1; 6.8 – 9.6 and 15 – 30 cm; respectively. In summary, shrimp production increased significantly with microbial augmentation, and water quality parameters were a suitable condition.
Straight to the Source? Where Adaptations, Artworks, Historical Films, and Novels Connect
Authors: Strong Jeremy 
Published: June 2019

AbstractResponding to several recent interventions in adaptation studies that have argued for history-as-adaptation, this article develops a sustained examination of how page-to-screen adaptations may be understood as structured and interpreted in ways analogous to the historical film. Considering the relationship between historical screen texts and the historical novel, including the many novel-to-film adaptations of such stories, the article identifies a distinct subset of adaptations in which artworks and literary works are engaged as the ‘source’ for fictional and semi-fictional narratives that ostensibly address the circumstances of their creation. Re-purposing the term ‘origin story’ to characterize these stories, the works of historical novelist Tracy Chevalier are posited as examples of this creative adaptive practice. In addition, this article argues for the trope of ‘bringing-to-life’ and the associated domain of re-enactment as key modes, deeply resonant since the earliest phases of cinema technology, for figuring both the page-to-screen adaptation and historical film. Finally, the 2015 historical biopic and adaptation Trumbo and its relationship to a range of sources are examined in the light of ideas proposed in this article.
Distributional Impacts of the Tax Cuts and Jobs Act Using Farm Household Microdata
Authors: Bawa Siraj G. , Williamson James M. 
Published: June 2019

AbstractThis paper uses ten years of USDA's Agricultural Resource Management Survey data to evaluate the impact of the Tax Cuts and Jobs Act (TCJA). We simulate income tax returns at the household level using the survey's detailed financial and demographic information. We find that farm households in the top 10% of the income distribution realize 71% of the total tax cuts from the TCJA, while families in the bottom 20% of the distribution experience a slight increase in their income tax liabilities. Consequently, the TCJA reduces the progressivity of the tax code, particularly over years with lower farm household income.
Density-Dependent Phenotypic Plasticity in the South American Locust, Schistocerca cancellata (Orthoptera: Acrididae)
Authors: Pocco Martina E , Cigliano M Marta , Foquet Bert , Lange Carlos E , Nieves Eliana L , Song Hojun 
Published: June 2019

AbstractHistorically, the South American locust, Schistocerca cancellata (Serville, 1838), has been considered the most serious agricultural pest in Argentina. An outbreak of a magnitude not recorded since 1954 started in 2015 through 2017 in northern Argentina and neighboring Paraguay and Bolivia. Schistocerca cancellata is widely considered as a true locust, with pronounced locust phase polyphenism, although the expression of its phenotypic plasticity has never been quantitatively tested under different density conditions. In this study, we explicitly quantified density-dependent reaction norms in behavior, coloration, and morphology in last instar nymphs of S. cancellata under isolated and crowded conditions. We also quantified density-dependent plasticity in adults (size) and in some life history traits. Our results showed that crowded nymphs were significantly more active and more attracted to congeners than isolated nymphs, and developed a much higher percentage of black pattern color. We also found that density had strong effects on body size and there was a sex-dependent pattern in both nymphs and adults, revealing that differences in size between males and females were less pronounced in crowded locusts. We have recorded for the isolated nymphs the presence of about 50% more hairs in the hind femora than in crowded nymphs. Finally, the mean duration of each nymphal instar and adult stage was significantly longer in isolated individuals. We have found strong resemblance with the desert locust, S. gregaria (Forskål, 1775) in several traits, and we conclude that S. cancellata exhibits an extreme form of density-dependent phenotypic plasticity in behavior, coloration, morphology, and life history traits.
Marriage stability in a pastoralist society
Authors: Du Juan , Mace Ruth 
Published: June 2019

AbstractWe examined how individual investment was associated with the duration of marriage partnerships in a pastoralist society of Amdo Tibetans in China. We collected demographic and socioeconomic data from 420 women and 369 men over five villages to assess which factors predicted partnership length. We found that the payment of dowry and bridewealth from both sides of the family predicted marriage stability. The production of offspring, regardless of their survivorship, also had a positive effect on marriage duration, as did trial marriage, a time period before formal marriage. Finally, we found that if both bride and groom invest resources initially into a partnership—whether wealth or labor—their subsequent partnership is stronger than couples who do not make such investments. This paper adds to our understanding of complex social institutions like marriage from a behavioral ecological perspective.
ACPred-Fuse: fusing multi-view information improves the prediction of anticancer peptides
Authors: Rao Bing , Zhou Chen , Zhang Guoying , Su Ran , Wei Leyi 
Published: June 2019

AbstractFast and accurate identification of the peptides with anticancer activity potential from large-scale proteins is currently a challenging task. In this study, we propose a new machine learning predictor, namely, ACPred-Fuse, that can automatically and accurately predict protein sequences with or without anticancer activity in peptide form. Specifically, we establish a feature representation learning model that can explore class and probabilistic information embedded in anticancer peptides (ACPs) by integrating a total of 29 different sequence-based feature descriptors. In order to make full use of various multiview information, we further fused the class and probabilistic features with handcrafted sequential features and then optimized the representation ability of the multiview features, which are ultimately used as input for training our prediction model. By comparing the multiview features and existing feature descriptors, we demonstrate that the fused multiview features have more discriminative ability to capture the characteristics of ACPs. In addition, the information from different views is complementary for the performance improvement. Finally, our benchmarking comparison results showed that the proposed ACPred-Fuse is more precise and promising in the identification of ACPs than existing predictors. To facilitate the use of the proposed predictor, we built a web server, which is now freely available via http://server.malab.cn/ACPred-Fuse.
Functional balance between Tcf21–Slug defines cellular plasticity and migratory modalities in high grade serous ovarian cancer cell lines
Authors: Varankar Sagar S , More Madhuri , Abraham Ancy , Pansare Kshama , Kumar Brijesh , Narayanan Nivedhitha J , Jolly Mohit Kumar , Mali Avinash M , Bapat Sharmila A 
Published: June 2019

Abstract
Cellular plasticity and transitional phenotypes add to complexities of cancer metastasis that can be initiated by single cell epithelial to mesenchymal transition (EMT) or cooperative cell migration (CCM). Our study identifies novel regulatory cross-talks between Tcf21 and Slug in mediating phenotypic and migration plasticity in high-grade serous ovarian adenocarcinoma (HGSC). Differential expression and subcellular localization associate Tcf21, Slug with epithelial, mesenchymal phenotypes, respectively; however, gene manipulation approaches identify their association with additional intermediate phenotypic states, implying the existence of a multistep epithelial-mesenchymal transition program. Live imaging further associated distinct migratory modalities with the Tcf21/Slug status of cell systems and discerned proliferative/passive CCM, active CCM and EMT modes of migration. Tcf21–Slug balance identified across a phenotypic spectrum in HGSC cell lines, associated with microenvironment-induced transitions and the emergence of an epithelial phenotype following drug exposure. Phenotypic transitions and associated functionalities following drug exposure were affirmed to ensue from occupancy of Slug promoter E-box sequences by Tcf21. Our study effectively provides a framework for understanding the relevance of ovarian cancer plasticity as a function of two transcription factors.
Tumor-derived exosomes promote carcinogenesis of murine oral squamous cell carcinoma
Authors: Razzo Beatrice M , Ludwig Nils , Hong Chang-Sook , Sharma Priyanka , Fabian Kellsye P , Fecek Ronald J , Storkus Walter J , Whiteside Theresa L 
Published: June 2019

Abstract
Circulating tumor-derived exosomes (TEX) interact with a variety of cells in cancer-bearing hosts, leading to cellular reprogramming which promotes disease progression. To study TEX effects on the development of solid tumors, immunosuppressive exosomes carrying PD-L1 and FasL were isolated from supernatants of murine or human HNSCC cell lines. TEX were delivered (IV) to immunocompetent C57BL/6 mice bearing premalignant oral/esophageal lesions induced by the carcinogen, 4-nitroquinoline 1-oxide (4NQO). Progression of the premalignant oropharyngeal lesions to malignant tumors was monitored. A single TEX injection increased the number of developing tumors (6.2 versus 3.2 in control mice injected with phosphate-buffered saline; P &lt; 0.0002) and overall tumor burden per mouse (P &lt; 0.037). The numbers of CD4+ and CD8+ T lymphocytes infiltrating the developing tumors were coordinately reduced (P &lt; 0.01) in mice injected with SCCVII-derived TEX relative to controls. Notably, TEX isolated from mouse or human tumors had similar effects on tumor development and immune cells. A single IV injection of TEX was sufficient to condition mice harboring premalignant OSCC lesions for accelerated tumor progression in concert with reduced immune cell migration to the tumor.
Age-Related Differences in Functional Asymmetry During Memory Retrieval Revisited: No Evidence for Contralateral Overactivation or Compensation
Authors: Roe James M , Vidal-Piñeiro Didac , Sneve Markus H , Kompus Kristiina , Greve Douglas N , Walhovd Kristine B , Fjell Anders M , Westerhausen René 
Published: June 2019

Abstract
Brain asymmetry is inherent to cognitive processing and seems to reflect processing efficiency. Lower frontal asymmetry is often observed in older adults during memory retrieval, yet it is unclear whether lower asymmetry implies an age-related increase in contralateral recruitment, whether less asymmetry reflects compensation, is limited to frontal regions, or predicts neurocognitive stability or decline. We assessed age-related differences in asymmetry across the entire cerebral cortex, using functional magnetic resonance imaging data from 89 young and 76 older adults during successful retrieval, and surface-based methods allowing direct homotopic comparison of activity between cortical hemispheres . An extensive left-asymmetric network facilitated retrieval in both young and older adults, whereas diverse frontal and parietal regions exhibited lower asymmetry in older adults. However, lower asymmetry was not associated with age-related increases in contralateral recruitment but primarily reflected either less deactivation in contralateral regions reliably signaling retrieval failure in the young or lower recruitment of the dominant hemisphere—suggesting that functional deficits may drive lower asymmetry in older brains, not compensatory activity. Lower asymmetry predicted neither current memory performance nor the extent of memory change across the preceding ~ 8 years in older adults. Together, these findings are inconsistent with a compensation account for lower asymmetry during retrieval and aging.
Slowly or Nonresolving Legionnaires’ Disease: Case Series and Literature Review
Authors: Pouderoux Cécile , Ginevra Christophe , Descours Ghislaine , Ranc Anne-Gaëlle , Beraud Laetitia , Boisset Sandrine , Magand Nicolas , Conrad Anne , Bergeron-Lafaurie Anne , Jarraud Sophie , Ader Florence 
Published: June 2019

Abstract

Background
Rarely, Legionnaires’ disease (LD) can progress into a slowly or nonresolving form.

Methods
A nationwide retrospective study was conducted by the French National Reference Center for Legionella (2013–2017) including cases of slowly or nonresolving LD defined as persistent clinical symptoms, computed tomography (CT) scan abnormalities, and Legionella detection in lower respiratory tract specimens by culture and/or real-time (RT) polymerase chain reaction (PCR) &gt;30 days after symptom onset.

Results
Twelve cases of community-acquired slowly or nonresolving LD were identified among 1686 cases of culture-positive LD. Median (interquartile range [IQR]) age was 63 (29–82) years. Ten (83.3%) patients had ≥1 immunosuppressive factor. Clinically, 9 patients transiently recovered before further deterioration (median [IQR] symptom-free interval, 30 [18–55] days), 3 patients had uniformly persistent symptoms (median [IQR] time, 48 [41.5–54] days). Two patients had &gt;2 recurrences. CT scan imagery found lung abscess in 5 (41.6%) cases. Slowly or nonresolving LD was diagnosed on positive Legionella cultures (n = 10, 83.3%) at 49.5 (IQR, 33.7–79) days. Two cases were documented through positive Legionella RT PCR at 52 and 53 days (cycle threshold detection of 21.5 and 33.7, respectively). No genomic microevolution and no Legionella resistance to antibiotics were detected. The median (IQR) duration of treatment was 46.5 (21–92.5) days. Two empyema cases required thoracic surgery. At a median (IQR) follow-up of 26 (14–41.5) months, LD-attributable mortality was 16.6% (n = 2).

Conclusions
Slowly or nonresolving LD may occur in immunocompromised patients, possibly leading to lung abscess and empyema.
Effective questioning in CLIL classrooms: empowering thinking
Authors: Valverde Caravaca Rebeca 
Published: June 2019

AbstractThis research explores the types of questions teachers ask their students in CLIL classrooms and how they can optimize their use of these questions. It came about as a result of a slowdown detected in the learning capacity of students after the first two years of CLIL programmes in the region of Murcia, Spain. The first data collected demonstrated that most classroom interactions only involved responding to display questions, which required the mere remembering or understanding of concepts. Hence, we implemented a testing methodology to determine whether stimulating higher-order thinking would result in a significant improvement in student outcomes. The results showed that this methodological intervention succeeded in its aim, as a marked improvement in student responses was achieved. These findings highlight the value of optimizing the use of questions to foster critical thinking and empower students for the knowledge economy in which we live.
Clinical safety of the ProMRI implantable cardioverter-defibrillator systems during head and lower lumbar magnetic resonance imaging at 3 T: results of the ProMRI 3T ENHANCED Master study
Authors: Zbinden Rainer , Wollmann Christian , Brachmann Johannes , Michaelsen Jochen , Steinwender Clemens , Kovoor Pramesh , Kelle Sebastian , McGavigan Andrew D , Ching Chi Keong , Figtree Gemma A , Schmidt Jan , Timmel Tobias , Lotz Joachim 
Published: June 2019

Abstract

Aims
There have been no published studies on the safety of magnetic resonance imaging (MRI) at 3 Tesla (3 T) in patients with MRI-conditional implantable cardioverter-defibrillators (ICDs). The aim of this study was to assess clinical safety of the Biotronik ProMRI ICD system during non-diagnostic head and lower lumbar scans under 3 T MRI conditions.

Methods and results
The study enrolled 129 patients at 12 sites in Australia, Singapore, and Europe. Predefined head and lower lumbar MR scans (total duration ≈30 min) were performed in 112 patients. Three primary endpoints were evaluated from the pre-MRI to the 1-month post-MRI visit: (i) freedom from serious adverse device effects (SADEs) related to MRI (hypothesized to be >90%); (ii) pacing threshold invariance for all leads (geometric mean of the patient-wise ratios for 1 month vs. pre-MRI was hypothesized to be <1.07); and (iii) sensing amplitude invariance (geometric mean of the ratios was hypothesized to be >0.993). No MRI-related SADE occurred (SADE-free rate 100%, 95% confidence interval 95.98–100%). Pacing threshold and sensing amplitudes fulfilled the invariance hypotheses with high statistical significance (P < 0.0013). No threshold increase >0.5 V or sensing amplitude decrease by >50% was observed (secondary endpoints). Lead impedances, battery capacity, and detection and treatment of arrhythmias by ICDs were not affected by MRI scans.

Conclusion
The head and lower lumbar scans under specific 3 T MRI conditions were safe in the investigated MR-conditional ICD systems. There was no evidence of harm to the patients or any negative influence of the MRI scan on the implanted systems.
Improving relative earthquake locations using surface-wave source corrections
Authors: Howe Michael , Ekström Göran , Nettles Meredith 
Published: June 2019

SUMMARYWe develop a procedure to improve estimates of relative earthquake locations using Rayleigh and Love wave arrivals for multiple earthquakes recorded at common stations. We fit predicted differential traveltimes to those measured using a cross-correlation technique, and correct the phases of the cross-correlation functions for phase delays that result from the surface-wave radiation patterns. We develop an empirical measure of uncertainty that provides realistic estimates of the errors in the earthquake locations. We investigate the effectiveness of the relocation procedure by first applying it to two suites of synthetic earthquakes. We then relocate real earthquakes in three separate regions: two ridge-transform systems and one subduction zone. We demonstrate that the inclusion of source corrections in the relocation procedure results in improved location estimates compared to relocations without source corrections. The source correction also allows for automated application of the relocation procedure, even in regions with a wide range of earthquake focal mechanisms.