bluuebunny/crossref_metadata_2025_split huggingface dataset:
A direct proof that sole actin dynamics drive membrane deformations
Authors: Simon Camille , Kusters Rémy , Caorsi Valentina , Allard Antoine , Abou-Ghali Majdouline , Manzi John , Di Cicco Aurélie , Lévy Daniel , Lenz Martin , Joanny Jean-François , Campillo Clément , Plastino Julie , Sens Pierre , Sykes Cécile 
Published: April 2018

AbstractCell membrane deformations are crucial for proper cell function. Specialized protein assemblies initiate inward or outward membrane deformations that turn into, for example, filopodia or endocytic intermediates. Actin dynamics and actin-binding proteins are involved in this process, although their detailed role remains controversial. We show here that a dynamic, branched actin network is sufficient, in absence of any membrane-associated proteins, to initiate both inward and outward membrane deformation. With actin polymerization triggered at the membrane of liposomes, we produce inward filopodia-like structures at low tension, while outward endocytosis-like structures are robustly generated regardless of tension. Our results are reminiscent of endocytosis in mammalian cells, where actin polymerization forces are required when membrane tension is increased, and in yeast, where they are always required to overcome the opposing turgor pressure. By combining experimental observations with physical modeling, we propose a mechanism for actin-driven endocytosis under high tension or high pressure conditions.
HIV Peptidome-Wide Association Study Reveals Patient-Specific Epitope Repertoires Associated with HIV Control
Authors: Arora Jatin , McLaren Paul J. , Chaturvedi Nimisha , Carrington Mary , Fellay Jacques , Lenz Tobias L. 
Published: April 2018

AbstractGenetic variation in the peptide-binding groove of the highly polymorphic human leukocyte antigen (HLA) class I molecules has repeatedly been associated with HIV-1 control and progression to AIDS, accounting for up to 12% of the variation in HIV-1 set point viral load (spVL). This suggests a key role in disease control for HLA presentation of HIV-1 epitopes to cytotoxic T cells. However, a comprehensive understanding of the relevant HLA-bound HIV epitopes is still elusive. Here we developed a peptidome-wide association study (PepWAS) approach that integrates HLA genotypes and spVL data from 6,311 HIV-infected patients to interrogate the entire HIV-1 proteome (3,252 unique peptides) for disease-relevant peptides. This PepWAS approach revealed a core set of epitopes associated with spVL, including previously characterized epitopes but also several novel disease-relevant peptides. More importantly, each patient presents only 16 (±7) and 6 (±6) of these core epitopes through their individual HLA-B and HLA-A variants, respectively. Differences in these patient-specific epitope repertoires account for almost all the variation in spVL previously associated with HLA genetic variation. PepWAS thus enables a comprehensive functional interpretation of the robust but little understood association between HLA and HIV-1 control, prioritizing a short and targetable list of disease-associated epitopes for personalized immunotherapy.
Ankyrin-G Regulates Forebrain Connectivity and Network Synchronization via Interaction with GABARAP
Authors: Nelson AD , Caballero-Florán RN , Rodríguez Díaz JC , Li J , Chen K , Walder KK , Bennett V , Lopez-Santiago LF , McInnis MG , Isom LL , Wang C , Zhang M , Jones KS , Jenkins PM 
Published: April 2018

ABSTRACTGABAergic circuits are critical for the synchronization and higher order function of brain networks, and defects in this circuitry are linked to neuropsychiatric diseases, including bipolar disorder, schizophrenia, and autism. Work in cultured neurons has shown that ankyrin-G plays a key role in the regulation of GABAergic synapses on the axon initial segment and somatodendritic domain of pyramidal neurons where it interacts directly with the GABAA receptor associated protein (GABARAP) to stabilize cell surface GABAA receptors. Here, we generated a knock-in mouse model expressing a mutation that abolishes the ankyrin-G/GABARAP interaction (Ank3 W1989R) to understand how ankyrin-G and GABARAP regulate GABAergic circuitry in vivo. We found that Ank3 W1989R mice exhibit a striking reduction in forebrain GABAergic synapses resulting in pyramidal cell hyperexcitability and disruptions in network synchronization. In addition, we identified changes in pyramidal cell dendritic spines and axon initial segments consistent with compensation for hyperexcitability. Finally, we identified the ANK3 W1989R variant in a family with bipolar disorder, suggesting a potential role of this variant in disease. Our results highlight the importance of ankyrin-G in regulating forebrain circuitry and provide novel insights into how ANK3 loss-of-function variants may contribute to human disease.
Policy compliance of SGB members on their financial management roles in selected no-fee schools in Limpopo province of South Africa
Authors: Dibete Kgabo Johannes , Potokri Onoriode Collins 
Published: April 2018

Purpose
The purpose of this paper is to investigate the perceptions of school governing bodies (SGBs) members’ role in financial management in no-fee schools so as to understand their compliance with policy frameworks when managing school finances. No-fee schools are schools in which the learners cannot be levied fees because of the poor socio-economic background of their parents, and are located in Quintiles 1-3 according to National Norms and Standards for School Funding Policy.

Design/methodology/approach
A qualitative research design/approach within the interpretive paradigm was adopted for the study. A sample of 22 participants from six selected no-fee schools was purposefully selected to participate in the study. The participants were principals, SGB chairpersons, SGB treasurers and finance officers. Data were collected through interviews and document analyses and were qualitatively analysed through coding and categorisation.

Findings
The findings reveal that the perceptions, experience and understanding of SGB members on their financial management roles differ. They lacked the proper knowledge to manage their funds effectively. This lack of knowledge is mainly because of the members’ literacy level and limited training.

Originality/value
This study offers a novel empirical and theoretical perspective on finance of no-fee schools and SGBs. This research subsequently recommends that rigorous training of chairpersons and treasurers should be done by department officials located in the finance section. Further, training should be conducted in a language that would be understood by the intended recipients.
Tacit knowledge-sharing behavior among the academic staff
Authors: Rahman Muhammad Sabbir , Mannan Mahafuz , Hossain Md Afnan , Zaman Mahmud Habib , Hassan Hasliza 
Published: April 2018

PurposeThe purpose of this paper is to examine the critical success factor of tacit knowledge-sharing behavior among the academic staff of higher learning institutions.Design/methodology/approachA total of 200 questionnaires were distributed among the academic staff of higher learning institutions in Bangladesh. Collectively, 150 usable responses were returned. Apart from descriptive statistics, this research applied confirmatory factor analysis (CFA) and structural equation modeling (SEM) to assess the measurement model and test the research hypotheses.FindingsThe findings from this empirical examination show that all the Big Five personality traits affect tacit knowledge-sharing behavior among the academic staff of higher learning institutions, except conscientiousness personality trait. However, the role of motivation, self-efficacy and mutual trust are also playing a significant role in tacit knowledge-sharing behavior. The CFA, SEM analysis confirmed the proposed conceptual model with a good model fit.Research limitations/implicationsThe findings of this research contribute to the understanding of the role of personality traits and other antecedents which play a significant role for the academic staff tacit knowledge-sharing behavior which are interesting for policymakers and authorities of higher learning institutions. As there was no sampling frame, the researchers applied convenient sampling technique in choosing the respondents.Originality/valueThis research plays a pioneering role where it contributes to the growing literature on the relationships between personality traits and other factors to construct a model for the tacit knowledge behavior by considering academic staff of higher learning institutions from a developing country’s perspective.
Professional identity of school teachers in the context of socio-historical dynamics of Russian society
Authors: Klimenko Ludmila , Posukhova Oxana 
Published: April 2018

Purpose
The purpose of this paper is to describe the specific nature of school teacher’s professional identity in the context of its stability maintenance in the Soviet and modern periods of Russian society development.

Design/methodology/approach
A total of 618 teachers of state comprehensive secondary schools were interviewed with a standardized questionnaire. The survey was divided into four semantic blocks: analyzing how teachers percept their own socio-economic situation; studying the structure of teacher’s social identity and determining the significance of professional identity; determining the nature of teacher’s motivation and professional values; and assessing the degree of labor precariatization.

Findings
This paper shows that a school teacher job had public prestige, social-labor and material guarantees, as well as ideological support from the state in the Soviet Russia. The excessive administrative burden, high social demands for teacher’s performance in the context of deteriorating economic situation in the country create risks for positive professional identity of teachers and, as a consequence, for societal integrity.

Originality/value
This study is relevant as it provides empirical measurements and substantiates macro-social effects of teacher’s professional identity. The excessive administrative load and high social demands for teacher’s performance in the context of deteriorating economic situation in the country create risks for maintaining the positive professional identity of teachers and, as a consequence, for societal integrity.
Classifying elements of a typology of gated communities
Authors: La Grange Adrienne 
Published: April 2018

Purpose
This paper aims to classify major elements in a typology of gated communities and develop a framework that can be used to promote international comparison of this built form.

Design/methodology/approach
The paper draws on a survey of 77 gated housing estates in Hong Kong and interviews with major stakeholders to develop and order a classification of elements of a typology of gated communities. Principle component analysis and regression analysis are used in conjunction with insights from 20 in-depth and about 70 open-ended face-to-face interviews.

Findings
This paper explores Hong Kong’s gated communities to evaluate the relationships between the four main elements of a typology of gated communities: supply, demand, features of gated estates and characteristics of built form. It is suggested that there is a hierarchical relationship between the elements, i.e. supply and demand are higher-order elements and features of gated housing and characteristics of the housing stock are lower-order elements. The paper additionally highlights the impact of definitional and conceptual drift in key concepts, such as security, privacy, prestige and lifestyle, on developing robust typologies.

Originality/value
The paper reviews the many and varied typologies of gated communities in the international literature, classifies the elements into four main groups and posits a hierarchical relationship between these elements. This paper proposes a robust methodology for further comparative research into gated communities.
Accountable care organizations: a strategy for future success?
Authors: Harrison Jeffrey , Spaulding Aaron , Harrison Debra A. 
Published: April 2018

Purpose
The purpose of this paper is to assess the community dynamics and organizational characteristics of US hospitals that participate in accountable care organizations (ACO).

Design/methodology/approach
Data were obtained from the 2015 American Hospital Association annual survey and the 2015 medicare final rule standardizing file. The study evaluated 785 hospitals which operate ACO in contrast to 1,446 hospitals without an ACO.

Findings
In total, 89 percent of hospitals using ACO’s are located in urban communities and 87 percent are not-for-profit. Hospitals with a higher case mix index are more likely to have an ACO.

Practical implications
ACOs allow healthcare organizations to expand their geographic markets, achieve greater efficiencies, and enhance the development of new clinical services. They also shift the focus of care from acute care hospitalization to the full continuum of care.

Originality/value
This research found ACOs with hospital and physician networks are an effective mechanism to control healthcare costs and reduce medical errors.
Impact of health sector reforms on hospital productivity in Turkey
Authors: Mollahaliloglu Saleh , Kavuncubasi Sahin , Yilmaz Fikriye , Younis Mustafa Z. , Simsek Fatih , Kostak Mustafa , Yildirim Selami , Nwagwu Emeka 
Published: April 2018

PurposeTurkish Ministry of Health (MoH) has Health Transformation Program (HTP). The purpose of this program has been to modify the structure of the current system in order to enhance health system productivity, quality, and access in the Turkish health system. The paper aims to discuss these issues.Design/methodology/approachTo measure the productivity, a data envelopment analysis-based Malmquist index approach was employed.FindingsResults showed that the overall HTP have had a considerable positive impact on the productivity of general hospitals.Research limitations/implicationsThe limitation is the availability of some data that might not be collected or reported to the MoH in Turkey.Practical implicationsThis research’s findings will have an impact on reforming the health care system in Turkey to be competitive and efficient as possible.Social implicationsThe research will have implication on reducing cost and provide value to the Turkish population.Originality/valueThis is one of the very few articles that targeted the efficiency of hospital system in Turkey.
The importance of urban rainwater harvesting in circular economy: the case of Guadalajara city
Authors: Gleason Espíndola José Arturo , Cordova Fernando , Casiano Flores Cesar 
Published: April 2018

Purpose
This paper aims to associate two fields of research: circular economy and the restoration of water cycle through the implementation of rainwater catchment systems in urban zones.

Design/methodology/approach
This study considers the case of the metropolitan zone of Guadalajara, México. This urban concentration is the second largest in Mexico. It faces floods each year with a cost of over US$26m, while demand of water has a production cost over US$24m. At the same time, the aquifers are drying due to uncontrolled urbanization and increasing the impervious area over the recharge zones. In addition, rainwater is combined with wastewater, elevating the cost of the wastewater treatment because the amount and quality of water to treat exceeds the systems’ capacity. This situation causes floods and decreases the availability of ground water. These problems are reflected in the imbalance of parameters of water cycle and a new approach is needed. The circular economy model can help to preserve one of our most vital resources. Scarcity is already so pronounced that we cannot reach many of our desired economic, social and environmental goals. Technologies that help balance supply and demand can also help water (both stock and flow) to become part of a circular model. To prove this, the authors present a hypothetical scenario based on a pilot project and a basin modeling of Guadalajara, Mexico.

Findings
Through this paper, it is possible to demonstrate that rainwater harvesting can play an important role in circular economy. Using the rainwater catchment systems, the cost of damages caused by floods could be decreased, the demand of water could be reduced, cost of production can be reduced, the aquifers can be recharged and the wastewater treatments can be improved.

Originality/value
Few papers have been developed to associate two fields of research (circular economy and the restoration of water cycle), using rainwater catchment systems as the central element.
Group structural elements in e-Government strategy formulation at local municipal level in South Africa
Authors: Osah Joshua , Khene Caroline 
Published: April 2018

Purpose
This study aims to identify group structural elements that should be assessed in e-Government strategy formulation processes at local government level for service delivery in South Africa. These elements influence the success or effectiveness of the strategy formulation process.

Design/methodology/approach
The theoretical framework of group structural elements by Asplund and nine other strategy formulation-related approaches are reflected upon. Weick’s approach of theory development is used to guide the theoretical analysis. A pragmatic approach is applied to conduct the empirical investigation using a case study of a digital citizen engagement project in South Africa.

Findings
The paper highlights the following group elements that influence the strategy formulation process: group member relations, degree of differentiation within the group and resources of the group. Not addressing these structural elements may result in a lack of creativity or a reluctance to participate in the context of public sector engagement.

Research limitations/implications
The paper does not provide an exhaustive list of all aspects that necessitate assessment before and during a strategy formulation process, for example, organisational and environment structural elements.

Practical implications
Assessing group structural elements provides valuable knowledge on how to engage stakeholders in a joint problem-solving activity. This is particularly pertinent where citizen engagement is fundamental to addressing basic service delivery concerns.

Originality/value
In the South African local municipal context, there is no existing literature that discusses the need to assess group structural elements influencing the outcome of an e-Government strategy formulation process.
Diagnostic criteria, severity classification and guidelines of localized scleroderma
Authors: Asano Yoshihide , Fujimoto Manabu , Ishikawa Osamu , Sato Shinichi , Jinnin Masatoshi , Takehara Kazuhiko , Hasegawa Minoru , Yamamoto Toshiyuki , Ihn Hironobu 
Published: April 2018

AbstractWe established diagnostic criteria and severity classification of localized scleroderma because there is no established diagnostic criteria or widely accepted severity classification of the disease. Also, there has been no clinical guideline for localized scleroderma, so we established its clinical guideline ahead of all over the world. In particular, the clinical guideline was established by clinical questions based on evidence‐based medicine according to the New Minds Clinical Practice Guideline Creation Manual (version 1.0). We aimed to make the guideline easy to use and reliable based on the newest evidence, and to present guidance as specific as possible for various clinical problems in treatment of localized scleroderma.
A host–parasite model explains variation in liana infestation among co‐occurring tree species
Authors: Visser Marco D. , Muller‐Landau Helene C. , Schnitzer Stefan A. , de Kroon Hans , Jongejans Eelke , Wright S. Joseph 
Published: April 2018

AbstractLianas are structural parasites of trees that reduce the growth, survival and reproduction of their hosts. Given that co‐occurring tree species differ strongly in the proportion of individuals that are infested by lianas (liana prevalence), lianas could differentially impact tree species and thereby influence tree community composition. Surprisingly, little is known about what governs variation in liana prevalence.Here, we apply an approach inspired by disease ecology to investigate the dynamics of liana prevalence over 11 years on Barro Colorado Island, Panama. We followed the fate of 1,938 individual trees from 21 tree species, recording deaths and change in liana infestation status. With these data, we fit species‐specific Markov chain models to estimate four rates: colonization by lianas (analogous to disease transmission), shedding or loss of lianas (analogous to host recovery), baseline mortality of uninfested trees (baseline mortality) and additional mortality of infested trees (parasite lethality).Models explained 58% of variation in liana prevalence among tree species, and revealed that host shedding of lianas and parasite lethality were the most important contributors to interspecific variation in liana prevalence at our site. These rates were also strongly related to shade tolerance, with light‐demanding species having greater rates of shedding and lethality, and lower rates of liana prevalence. An indirect path analysis with a structural equation model revealed that both greater rates of liana shedding and liana‐induced lethality contribute to the observed lower rates of liana prevalence for light‐demanding tree species.Synthesis. Our approach revealed that the prevalence of liana infestation among tree species is driven via indirect pathways operating on the rates of shedding and lethality, which relate to the ability (or inability) of trees to shed and/or tolerate lianas. Shade‐tolerant trees have greater proportions of trees infested by lianas because they are both less able to shed lianas and more able to tolerate infestation.
Global diversity and distribution of close relatives of apicomplexan parasites
Authors: Mathur Varsha , del Campo Javier , Kolisko Martin , Keeling Patrick J. 
Published: April 2018

SummaryApicomplexans are a group of obligate intracellular parasites, but their retention of a relict non‐photosynthetic plastid reveals that they evolved from free‐living photosynthetic ancestors. The closest relatives of apicomplexans include photosynthetic chromerid algae (e.g., Chromera and Vitrella), non‐photosynthetic colpodellid predators (e.g., Colpodella) and several environmental clades collectively called Apicomplexan‐Related Lineages (ARLs). Here we investigate the global distribution and inferred ecology of the ARLs by expansively searching for apicomplexan‐related plastid small ribosomal subunit (SSU) genes in large‐scale high‐throughput bacterial amplicon surveys. Searching more than 220 million sequences from 224 geographical sites worldwide revealed 94 324 ARL plastid SSU sequences. Meta‐analyses confirm that all ARLs are coral reef associated and not to marine environments generally, but only a subset is actually associated with coral itself. Most unexpectedly, Chromera was found exclusively in coral biogenous sediments, and not within coral tissue, indicating that it is not a coral symbiont, as typically thought. In contrast, ARL‐V is the most diverse, geographically widespread and abundant of all ARL clades and is strictly associated with coral tissue and mucus. ARL‐V was found in 19 coral species in reefs, including azooxanthellate corals at depths greater than 500 m. We suggest this is indicative of a parasitic or commensal relationship, and not of photosynthetic symbiosis, further underscoring the importance of isolating ARL‐V and determining its relationship with the coral host.