bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of autologous platelet-rich plasma intrauterine perfusion on clinical outcomes in recurrent implantation failure patients with non-thin endometrium undergoing frozen-thawed embryo transfer
Authors: Liu Xiao-Hui , He Li-Xia , Li Man , Tian Zheng-Ping , Qin An-Qi , Yao Jun 
Published: November 2024

Abstract

Purpose To explore the effects of autologous platelet-rich plasma (PRP) intrauterine perfusion on clinical outcomes in recurrent implantation failure (RIF) patients with non-thin endometrium undergoing frozen-thawed embryo transfer (FET), and the effects of PRP used at different times before FET on clinical outcomes. Methods A total of 160 RIF patients with non-thin endometrium undergoing FET were retrospectively analyzed. Among them, 82 patients received PRP intrauterine perfusion at 24–72 hours before FET(PRP group), and 78 patients did not (non-PRP group). In PRP group, 59 patients underwent PRP at 24 ~ 48h before FET(≥ 24 ~ ≤ 48h group), and 23 patients was at 48 ~ 72h (> 48 ~ ≤ 72h group). The clinical outcomes were compared, including β-hCG positive rate, clinical pregnancy rate, embryo implantation rate, abortion rate, ectopic pregnancy rate, live birth rate and the incidence of adverse events. Results The clinical pregnancy rate, embryo implantation rate and live birth rate in PRP group were significantly higher than those in non-PRP group (P < 0.05), and there were no statistical differences in β-hCG rate, abortion rate and ectopic pregnancy rate between the two groups (P > 0.05). Meanwhile, there was no adverse events occurred in PRP group. However, the C-type endometrium rate in PRP group was observably higher on FET day (X2 = 8.309, P = 0.004), though there was no statistical difference in endometrial thickness (P > 0.05). The multiple logistics regression analysis showed that PRP intrauterine perfusion are closely related with clinical pregnancy and live birth in RIF patients with non-thin endometrium (OR:2.379, 95%CI:1.137 ~ 4.977, P = 0.021; OR:2.107, 95% CI:1.006 ~ 4.412, P = 0.048). Moreover, we found no significant difference in clinical outcomes between the two groups of PRP intrauterine perfusion at ≥ 24 ~ ≤ 48h and > 48 ~ ≤ 72h before FET (P > 0.05), except for β-hCG positive rate. Conclusions PRP intrauterine perfusion can safely and effectively improve the clinical pregnancy rate, implantation rate and live birth rate in RIF patients with non-thin endometrium possibly by increasing the C-type endometrium rate on FET day. In addition, PRP intrauterine perfusion at different times of 24 ~ 72 hours before FET does not affect the clinical outcomes, which will be helpful to guide clinical work flexibly.
Research Article Surgical Outcomes of Cesarean Sections after Laparoscopic Myomectomy: A Comparison with Open Myomectomy and Other Cesarean
Authors: Kotani Yasushi , Moriuchi Kaori , Sakamoto Takaya , Wada Chiharu , Fukuda Naho , Koh Iiji , Murakami Kosuke , Takaya Hisamitsu , Kawasaki Kaoru , Matsumura Noriomi 
Published: November 2024

Abstract

Background
Myomas are a common gynecologic disease. Laparoscopic myomectomy procedures have been performed more frequently in recent years. We were unable to find any reports pertaining to actual surgical findings such as adhesions during cesarean section after laparoscopic myomectomy. We compared surgical outcomes at the time of cesarean section in individuals who underwent laparoscopic myomectomy at our hospital with those who other cesarean sections.
Methods
From 1995 to 2023, the surgical outcomes of 49 patients who underwent laparoscopic myomectomy at our hospital and later underwent cesarean section for singleton term delivery were compared with those of 20 singleton full term cesarean sections after open myomectomy, 86 singleton full term previous cesarean sections without previous laparotomy, and 43 cesarean sections after breech delivery. In addition, patient background and surgical outcomes for cesarean sections after laparoscopic myomectomy and open myomectomy were compared between the two groups.
Results
In a comparison of blood loss among the four groups, cesarean sections after laparoscopic myomectomy and open myomectomy resulted in significantly more blood loss than breech position cesarean sections. On the other hand, a comparison between laparoscopic myomectomy and open myomectomy showed that the recurrence rate of uterine fibroids upon cesarean section was significantly higher after laparoscopic myomectomy. Adhesions at cesarean section were more common with open myomectomy and myometrial dimpling/thinning was more frequent after laparoscopic myomectomy, but the difference was not statistically significant. There were no cases of uterine rupture during pregnancy in our patients.
Conclusions
Practitioners performing cesarean sections need to be very careful about the incidence of bleeding during cesarean sections after laparoscopic myomectomy. Also, practitioners performing laparoscopic myomectomy need to be very careful about adhesion, dimpling, thinning and recurrence.
Multiparametric radiomics and prognostic nutritional index for predicting postoperative survival in esophageal carcinoma
Authors: Luo Weiwei , Dong Jindong , Deng Jiaying , Tong Tong , Chen Xiangxun , Wang Yichun , Wang Fan , Zhu Liyang 
Published: November 2024

Abstract

Background
Surgery can lead to radical cure and long-term survival for individuals with esophageal squamous cell carcinoma (ESCC). Nevertheless, the survival rates markedly vary among patients. Accurately predicting surgical efficacy remains a pressing issue. This investigation sought to examine the predictive value of preoperative radiomics and the prognostic nutritional index for individuals with ESCC and to construct a comprehensive model for estimating the postoperative overall survival (OS) of individuals with ESCC.
Methods
This research conducted a retrospective examination of 466 individuals with ESCC from two medical centers. The data were arbitrarily categorized into a training cohort (TC, hospital 1, 246 cases), an internal validation cohort (IVC, hospital 1, 106 cases), and an external validation cohort (EVC, hospital 2, 114 cases). Upon demarcation of the area of interest, radiological features were extracted. The least absolute shrinkage and selection operator (LASSO) regression was utilized to identify the optimal radiomics features and calculate the radiomics score (RS). After the delineation of region of interest, radiological features were procured. Subsequently, the LASSO regression was employed to ascertain the optimal features and calculate the RS. The independent influencing factors acquired through Cox analyses were incorporated with the RS to establish a combined nomogram. The predictive capability of the model was examined utilizing the concordance index, time-dependent receiver operating characteristic (ROC) curves, calibration plots, and decision curve analysis.
Results
In the predictive model integrating preoperative radiomics with prognostic nutritional index and tumor–node–metastasis (TNM) stage for forecasting the 3-year OS rate, the area under the time-dependent ROC curve (AUC) was 0.812, 0.748, and 0.810 in the TC, IVC, and EVCs, respectively, thereby demonstrating outstanding prognostic significance. This was superior to the AUC values of the TNM stage prediction model in the TC, IVC, and EVCs, which were 0.717, 0.612, and 0.699, respectively. The concordance indexes of the combined model in the TC, IVC, and EVCs were 0.780, 0.760, and 0.764, respectively. The calibration and decision curves illustrated the nomogram’s remarkable calibration performance and clinical application value.
Conclusion
In this investigation, a predictive model was developed by integrating radiomics and the prognostic nutritional index. This model can predict the OS rate of postoperative patients with ESCC and could be employed as a tool for preoperative risk stratification.
Comparison of Radioisotope Adsorption Capability in Metal Organic Frameworks through DFT Simulation
Authors: Lee Hwanhui , An Jeongho , Chang Kunok 
Published: November 2024

Abstract

We compared the adsorption performance of graphene and MOF-303 for three radionuclides of significant concern in radioactive waste: Co, Sr, and Cs, using density functional theory. Additionally, we doped MOF-303 with transition metals such as Cu, Ag, and Au by replacing the hydrogen atoms in the nitrogen linker, and investigated the resulting changes in adsorption capability. The adsorption capability of MOF-303 for the Co, Sr, and Cs was superior to the adsorption capability of graphene. Furthermore, doping MOF-303 with Cu, Ag, and Au further enhanced the adsorption capability of MOF-303 for Co, Sr, and Cs. Ag-MOF-303 exhibited significant improvement in Sr atom adsorption at specific sites. The adsorption improvement is likely due to the formation of new bonds between Ag-MOF-303 and the Sr atom, resulting from Ag doping. The assumption of new bond formation was confirmed by an increase in the density of states. While Ag-MOF-303 demonstrated superior performance in Sr atom adsorption at specific site, Au-MOF-303 resulted in the most significant overall increase in adsorption energy for Co, Sr, Cs among the Cu-MOF-303, Ag-MOF-303, and Au-MOF-303.
Transgenic Eucalyptus urophylla × Eucalyptus grandis Superior Clone DH3229: Achieving Glyphosate Resistance through Genetic Modification
Authors: Wang Xuejun , Wei Haoyu , Zhang Shuang , Gao Jiahui , Zeng Binshan , Fan Chunjie 
Published: November 2024

Abstract

Eucalyptus species are known for their high productivity and adaptability, making them a reliable source of timber. However, hybrid eucalyptus, particularly in the initial months of plantation, is susceptible to weed competition. Glyphosate is the most commonly used herbicide for weed control in eucalyptus plantations. To incorporate glyphosate resistance into eucalyptus cultivars (Eucalyptus urophylla × E. grandis DH3229), the g10-epsps gene, driven by the 35S promoter, was introduced using an efficient Agrobacterium-mediated genetic transformation method. Following PCR-based selection and detection, six plants were confirmed to have successfully integrated the epsps gene into the eucalyptus genome at various stages. Realtime fluorescence quantitative PCR and enzyme-linked immunosorbent assay further validated the gene's integration and expression in all independent transformation events. Additionally, the transgenic plants demonstrated significant resistance to glyphosate after being treated with a 200-fold diluted Roundup solution. Physiological analysis revealed that maintaining lower levels of shikimic acid in transgenic plants is crucial for glyphosate tolerance. The successful development of herbicide-resistant transgenic eucalyptus in elite clones presents significant potential for future breeding programs and may be directly applicable to plantation after further field testing.
Probiotic Intervention Alleviates Helicobacter pylori-induced Intestinal Inflammation by Sustaining Intestinal Homeostasis
Authors: Wu Shiying , Wei Fangtong , Chen Yongqiang , Chen Ziqi , Luo Yuenuo , Fan Jiayi , Xu Yang , Hu Mingyang , Li Ping , Gu Qing 
Published: November 2024

Abstract

Helicobacter pylori (H. pylori) infection poses significant risks for gastric cancer and intestinal inflammation, yet effective prevention strategies for intestinal inflammation remain elusive. Here, we aimed to investigate the protective effects and underlying mechanisms of Lactiplantibacillus plantarum ZJ316 (L. plantarum ZJ316) in a mouse model of H. pylori-induced intestinal inflammation. Our results demonstrated that treatment with L. plantarum ZJ316 effectively reduced tissue damage and upregulated expression of tight junction proteins such as Zonula occludens-1 (ZO-1), Occludin, and Claudin-1, while decreased pro-inflammatory cytokines interleukin-1β (IL-1β), interferon γ (IFN-γ), and tumor necrosis factor α (TNF-α). Additionally, intaking L. plantarum ZJ316 reduced relative abundance of pathogenic bacteria Staphylococcus and Desulfovibrio by 69%, and 42%, respectively, while enhancing beneficial bacteria including Ligilactobacillus, Akkermansia, and Lactobacillus associated with short-chain fatty acids (SCFAs) synthesis, by 88%, 85%, and 16%, respectively. Gas chromatography–mass spectrometry (GC-MS) analysis confirmed L. plantarum ZJ316 reversed H. pylori-induced declines in SCFA levels. In vitro, L. plantarum ZJ316 inhibited the IκBα/NF-κB pathway, thereby reducing TNF-α and IL-8 production in HT-29 cells following H. pylori infection. These findings collectively suggest that L. plantarum ZJ316 ameliorates H. pylori-induced intestinal inflammation by enhancing gut barrier function, improving flora structure, increasing SCFA levels, and mitigating inflammation through NF-κB pathway inhibition, offering promise for therapeutic development.
Effects of forest fire disturbance on non-structural carbohydrates and carbon, nitrogen and phosphorus of Pinus yunnanensis
Authors: Fu Xiaoyong , Kou Weili , Wei Lili 
Published: November 2024

Abstract

The organ-level effects of wildfire on plant nonstructural carbohydrates (NSC) and stoichiometric characteristics are poorly understood. We studied the effects of fire on sugar, starch, carbon (C), nitrogen (N), and phosphorus (P) content in the needles, branches, stems, and fine roots of fire-damaged Pinus yunnanensis trees (F-trees). Starch, sugar, and NSC content were higher in the roots and needles of F-trees than that of undamaged individuals (H-trees). C, N, and P content and stoichiometry characteristics of F-trees also differed significantly from H-trees. The N/P content of both F-tree and H-tree needles were < 14 prior to forest fire disturbance and increased thereafter, indicating that N limitation increased after fire disturbance. In addition, F-tree physiology did not return to control levels nine months after fire disturbance, indicating that more time is needed to recover. These findings suggest that forest management practices that increase soil N content will likely accelerate post-fire recovery. Because fire frequency and intensity will continue to increase in response to climate change, our results are of great value in improving our understanding of post-fire ecosystem dynamics.
Akkermansia muciniphila exacerbates acute radiation-induced intestinal injury by compromising the mucosal barrier
Authors: Ding Yi , Wang Yafang , Wang Xusheng , Chen Zhenhui , Zheng Jihua , Liu Xiangqiang , Zheng Yilin , Zheng Zhihao , Xu Zi , Zhang Yaowei , Chen Keli , Zhang Yuqin , Yu Lu 
Published: November 2024

Abstract

Dysbiosis of gut microbiota plays a crucial role in acute radiation-induced intestinal injury (ARIII). However, studies on the influence of gut microbiota on ARIII are inconsistent. In this study, we established an ARIII mouse model and performed fecal microbiota transplantation (FMT) to explore the role of the gut microbiota in ARIII. We observed a significant increase in Akkermansia muciniphila (AKK) following irradiation, whereas FMT effectively reduced AKK levels. Contrary to expectations, AKK supplementation increased ARIII and mortality. Mechanistically, post-radiation AKK upregulates mucin metabolism genes and consumes mucin, thinning the mucosal barrier and promoting the adhesion and translocation of potential pathogens to epithelial cells, thus exacerbating ARIII. This enables AKK to use mucin as an energy source. Additionally, AKK increases the infiltration of M1-type macrophages and secretion of inflammatory cytokines, leading to a decrease in epithelial stem cell density and inhibition of goblet cell differentiation, further exacerbating ARIII. Our findings suggest that in certain intestinal environments, the addition of AKK may worsen radiation-induced intestinal damage; thus, the potential of AKK as a universal probiotic should be considered with caution.
Contrasting energy flow associated with tropical and subtropical reef fish assemblages
Authors: Liu Chia-Hung Eric , Ribas-Deulofeu Lauriane , Wu Meng-Hsin Morris , Chang Yi-Jay , Denis Vianney 
Published: November 2024

Abstract

Energy flows (production and consumption of biomass) are key to ecosystem functioning and resilience. In coral reefs, fishes are involved in core ecological processes associated with energy flow, and their rapid responses to biotic and abiotic changes further make them ideal indicators for assessing coral reef health. However, variations in fish energy flow across a natural gradient have been overlooked, yet could be important for interpreting changes in ecosystem functioning. Here, fish assemblages were surveyed by stereo-video transects in five regions spanning a tropical-subtropical transition and were further characterized using three energy flow metrics (standing biomass, productivity, and turnover). Generalized linear mixed models were used to identify intrinsic (biotic; i.e., benthic morpho-functional groups) and extrinsic (abiotic; i.e., environmental and anthropogenic factors) drivers of fish energy flow. The results showed regional specificities in the patterns of fish energy flows. The subtropical region showed a dominance of planktivorous fishes characterizing a middle-driven trophic system with high energy flows. In contrast, transitional and tropical regions were dominated by parrotfishes and surgeonfishes while exhibiting a concave trophic structure lacking high-level predators. The intrinsic and extrinsic drivers of fish energy flow suggested that crustose coralline algae, encrusting hard corals, and the presence of hard substrates and crevices may leverage the maintenance of ecosystem functioning. Overall, this study identifies the characteristics of energy flows between subtropical and tropical regions and reveals the influential intrinsic and extrinsic factors on these flows. It further establishes baseline information for interpreting the consequences of climate change on reef ecosystem functioning.
Fragmentation of fibrous particles in LC refining
Authors: Niskanen Kaarlo , Berg Jan-Erik , Liubytska Kateryna , Sandberg Christer 
Published: November 2024

Abstract

Low-consistency (LC) refining is used both in mechanical pulping and in general to give a final touch to a papermaking pulp. The desired – and undesired - effects come from changes in the property and size distributions of the different kinds of fibrous particles. In our study, we focus on the changes in the size distributions of fibre length and diameter, measured with an optical fibre analyser. Pulp samples were collected before and after industrial LC refiners of thermomechanical pulps with spruce as raw material. We demonstrate that changes in the observed size distributions can be reproduced with a model with just two parameters for particles breaking uniformly at random locations. One probability controls the breaks per unit length that shorten fibres, and another the splits per unit diameter that generate more fines. Our findings support the hypothesis that these two processes are separate so that breaks in length do not govern the increase in fines. For example, both fibre shortening and fines generation increased with refining energy but, at a given energy, only fibre shortening showed clear differences between trials. In a two-stage refining trial, the probability that fibres shorten fell on the same line as the one-stage probabilities when plotted against the refining energy measured from the threshold energy at which breaks start.
Impacts of the Carceral Environment on Venous Thromboembolism Mortality
Authors: Valenzuela Lisette , Hasan Muryam , Brandt Nikolas , Woods Amy , Samra Shamsher , Bhatia Nona , Shapiro Nicholas 
Published: November 2024

Abstract

Background: Venous thromboembolism (VTE), encompassing deep vein thrombosis and pulmonary embolism, is a major cause of mortality globally and has shown an increased incidence in the United States. Despite this, no research has sought to identify VTE risk factors in carceral settings. This study investigates the prevalence of VTE-related deaths in jailed and non-jailed populations in Los Angeles County over a 16-year period (2008 to 2023).
Methods: We used death records to compile two datasets: one for deaths that occurred in Los Angeles County jails (n = 512) and another for deaths that occurred in the free population (n = 107,608). VTE cases were identified through a keyword search and reviewed to confirm consistency with VTE diagnostic criteria. Statistical analyses were conducted to compare mortality rates and demographic characteristics between populations. Additionally, we performed a qualitative review of death investigation records for jailed VTE decedents to identify potential risk factors associated with the carceral environment.
Results: VTE-related deaths in the jailed population were more than twice as high as those in the free population (3.6% vs. 1.1%, p = 0.0014). VTE crude mortality rates in jails have been increasing since 2014, with the highest rate observed in 2021. Black individuals constitute 7.9% of the county’s population, 29.4% of the jail population, and 66.67% of VTE deaths in jails. Jail appears to heighten existing racial disparities in VTE health outcomes.
Conclusions: Our findings suggest that incarceration may be associated with higher rates of VTE-related mortality, particularly among Black people and people with mental health conditions. Structural and environmental factors within carceral settings, such as restricted mobility, antipsychotic medication usage, and restraint may contribute to these elevated risks. Improving reporting of restraint use and post-mortem toxicology findings for pulmonary embolism deaths will deepen the public understanding of this issue. Diversion for mental health populations, and other decarceral programs can mitigate VTE risk and reduce health inequities. Prescription of anticoagulants may additionally mitigate antipsychotic and restraint VTE risk.
Prevalence and risk factors associated with gastrointestinal helminth infections in the Mt Elgon subregion: A cross sectional study in Bulambuli District, Eastern Uganda
Authors: Wogoli Geoffrey , Opiro Robert , Okot Jacob , Olia Alex , Echodu Richard , Alarakol Simon Peter 
Published: November 2024

Abstract

Background Gastrointestinal helminths are considerable constraints in cattle farming in Sub-Saharan Africa causing significant ill health and economic losses to rural communities. This study aimed to determine the prevalence and factors associated with gastrointestinal helminths in cattle in Bulambuli District, Eastern Uganda.Methods A cross-sectional study was conducted on 324 randomly sampled cattle from April 2022 to July 2023 in Bulambuli District. Fecal and blood samples collected were analysed via a combination of simple floatation, sedimentation, and haematocrit centrifugation techniques. Data on sociodemographic characteristics were collected and analysed using descriptive statistics and logistic regression.Results Overall, 197 (60.8%) cattle were positive for gastrointestinal helminth infections. Thirteen helminth species were identified; Cooperia punctata (20%), Ostertagia ostertatagi, (18%), and Haemonchus contortus (18%) were the predominant species. Thirty nine (12.0%) cattle had mixed infections mainly of Cooperia spp and Paramphistomum spp (13.8%). Ninety-one, (28.1%) cattle had mono-infections with Ostertagia species being the most predominant species. The variables significantly associated with helminthic infections were; keeping crossbred cattle; (AOR = 1.8 [95%CI = 1.2–1.8], p = 0.01), delayed cleaning of barns (AOR = 4.89 [95%Cl = 2.66-9.0], p = 0.01) and overcrowding (AOR = 3.12[95%Cl = 155 − 6.3], p = 0.01). All the key informants agreed that environment, poor management practices, and breed were key factors that could influence GIHs infections among cattle in Bulambuli district.Conclusion Cattle in Bulambuli have high levels of helmithic infections, presenting a big threat to the health of animals and economic productivity of communities in the district. There is need to design and implement tailored control strategies that take into account the identified risk factors to mitigate the health risks and economic losses resulting from GIH infections in the Mt Elgon subregion.