bluuebunny/crossref_metadata_2025_split huggingface dataset:
MicroRNA-506-3p initiates mesenchymal-to-epithelial transition and suppresses autophagy in osteosarcoma cells by directly targeting SPHK1
Authors: Wang Dapeng , Bao Fuqin , Teng Yugang , Li Qiang , Li Jianjun 
Published: January 2019

ABSTRACT
Osteosarcoma (OS) is the most common malignant bone tumor. In cancer cells, autophagy is related to epithelial-to-mesenchymal transition (EMT). Although microRNA (miR)-506-3p has been demonstrated to act as a tumor suppressor in OS, its role in regulating the EMT process and autophagy remains unknown. The results showed that miR-506-3p directly inhibited the expression of sphingosine kinase 1 (SPHK1) in 143B and SaOS-2 cells. The invasive capability of OS cells was reduced following miR-506-3p mimics transfection, and restored when SPHK1 was overexpressed simultaneously. Further, miR-506-3p mimics initiated mesenchymal-to-epithelial transition (MET) – E-cadherin expression was upregulated, whilst vimentin and fibronectin were downregulated. The basal autophagy flux (LC3II/I) was suppressed by miR-506-3p mimics. The alterations induced by miR-506-3p mimics were partly reversed by SPHK1 overexpression or treatment of rapamycin. Meanwhile, treatment of SPHK1-transfected cells with 3-methyladenine inhibited EMT. The data suggest that miR-506-3p initiates MET and suppresses autophagy in OS cells by targeting SPHK1.
Expression and characterization of recombinant human lactoferrin in edible alga Chlamydomonas reinhardtii
Authors: Pang Xiaonan , Tong Yuxi , Xue Wenzhi , Yang Yi-feng , Chen Xiwen , Liu Jia , Chen Defu 
Published: January 2019

ABSTRACT
Lactoferrin (LF) is a naturally occurring iron-binding glycoprotein with a variety of biological functions. It has increasing demand every year and huge market potential. In this study, we explored the feasibility of expressing human LF (hLF) in edible algae C. reinhardtii. A codon-optimized hLF gene was synthesized, inserted into pCAMBIA-1301C and transformed into C. reinhardtii SP strain. In total, 7 hLF-expressing clones were selected with clone 121 exhibiting the highest expression level. The hLF-containing algal extract significantly inhibited the growth of bacteria such as Escherichia coli and Klebsiella variicola. During acute toxicity experiment no acute toxicity was detected, especially on changes of the body weight and histopathology of organs. The recombinant hLF possessed a similar or modestly reduced stability compared to commercial hLF standard. Our data indicated that expression of hLF in C. reinhardtii is feasible and paved a way to commercial production of lactoferrin using edible Chlamydomonas expression system.
Abbreviations: atrazine chlorohydrolase gene (atzA); bovine serum albumin (BSA); human LF (hLF); lactoferrin (LF); Luria-Bertani (LB); quantitative reverse transcriptase PCR (qRT-PCR) ; SDS polyacrylamide gel electrophoresis (SDS-PAGE); Tris-acetate phosphate (TAP); western blotting (WB)
‘Photo‐words’: promoting language skills using photographs
Authors: Walter Ofra , Gil-Glazer Ya'ara , Eilam Billie 
Published: January 2019

AbstractThis study, using a mixed methods design, examined the use of a photograph‐based model to improve students’ language and writing skills. Classes were assigned to receive the photograph‐based intervention (experimental) or to receive the regular language‐based curriculum (control). Both second and fifth grade students in the experimental group significantly improved their language and writing skills compared to the control group. Second grade students improved significantly more than fifth grade students. The findings also enabled the research team to identify deficiencies that required specific modifications in language curriculum design.
Barriers to Receiving Support among Young Arab Women in Israel Who Have Been Abused in Childhood
Authors: Elias Haneen , Alnabilsy Raghda , Pagorek-Eshel Shira 
Published: January 2019

AbstractYoung Arab women (YAW) in Israel who have been abused in childhood may experience barriers to both formal and informal support as an ethnic minority and a gender exposed to exclusion, discrimination and oppression. However, only scant reference has been made to this problem in the literature. Here, in a qualitative study of twenty YAW in Israel who had experienced abuse in childhood, we explored their perception of barriers to support following the abuse. Theoretical understandings were developed using a grounded theory approach. The ecological theory and the intersection of the marginal positions theory further developed emergent theoretical understandings during analysis. The main findings of the study are based on three major meta-categories: sociopolitical barriers, sociocultural barriers and intra-interpersonal barriers. These meta-categories, which create a mechanism of silencing the abuse, reflect a cumulative effect of the barriers ranging from macro-levels to micro-levels. The article discusses the implications of the findings on social policy and social work training in order to enhance culturally- and gender-adapted treatment programmes.
A Double Dissociation in Sensitivity to Verb and Noun Semantics Across Cortical Networks
Authors: Elli Giulia V , Lane Connor , Bedny Marina 
Published: January 2019

AbstractWhat is the neural organization of the mental lexicon? Previous research suggests that partially distinct cortical networks are active during verb and noun processing, but what information do these networks represent? We used multivoxel pattern analysis (MVPA) to investigate whether these networks are sensitive to lexicosemantic distinctions among verbs and among nouns and, if so, whether they are more sensitive to distinctions among words in their preferred grammatical class. Participants heard 4 types of verbs (light emission, sound emission, hand-related actions, mouth-related actions) and 4 types of nouns (birds, mammals, manmade places, natural places). As previously shown, the left posterior middle temporal gyrus (LMTG+), and inferior frontal gyrus (LIFG) responded more to verbs, whereas the inferior parietal lobule (LIP), precuneus (LPC), and inferior temporal (LIT) cortex responded more to nouns. MVPA revealed a double-dissociation in lexicosemantic sensitivity: classification was more accurate among verbs than nouns in the LMTG+, and among nouns than verbs in the LIP, LPC, and LIT. However, classification was similar for verbs and nouns in the LIFG, and above chance for the nonpreferred category in all regions. These results suggest that the lexicosemantic information about verbs and nouns is represented in partially nonoverlapping networks.
The PFAD-HEC Model: Impacts of News Attributes and Use Motivations on Selective News Exposure
Authors: Mothes Cornelia , Knobloch-Westerwick Silvia , Pearson George D H 
Published: January 2019

Abstract
This study examined effects of four common news attributes—personalization, fragmentation, authority–disorder bias, and dramatization (PFAD)—on news exposure and the moderating impacts of hedonic, epistemic, and civic news use motivations. In a lab experiment, participants browsed online news while selective exposure was unobtrusively logged. Findings yielded longer exposure to personalized and dramatized articles and news with low authority–disorder bias. Fragmentation had no significant impact. However, selective exposure to fragmented news was influenced by participants’ political understanding (epistemic motivation), exposure to personalization by news enjoyment (hedonic motivation), and exposure to authority–disorder bias by civic duty to keep informed (civic motivation). Results suggest that news styles may need to become more diversified to better address the informational needs of today’s fragmented audiences.
An elastic harmonic inclusion near a rigid harmonic inclusion loaded by a couple
Authors: Wang Xu , Chen Liang , Schiavone Peter 
Published: January 2019

AbstractWe use conformal mapping techniques to solve the inverse problem concerned with an elastic non-elliptical harmonic inclusion in the vicinity of a rigid non-elliptical harmonic inclusion loaded by a couple when the surrounding matrix is subjected to remote uniform stresses. Both a size-independent complex loading parameter and a size-dependent real loading parameter are introduced as part of the solution procedure. The stress field inside the elastic inclusion is uniform and hydrostatic; the interfacial normal and tangential stresses as well as the hoop stress on the matrix side are uniform along each one of the two inclusion–matrix interfaces. The tangential stress along the interface of the elastic inclusion (free of external loading) vanishes, whereas that along the interface of the rigid inclusion (loaded by the couple) does not. A novel method is proposed to determine the area of the rigid inclusion.
Diet May Drive Influenza A Virus Exposure in African Mammals
Authors: Soilemetzidou Eirini-Sanatana , De Bruin Erwin , Franz Mathias , Aschenborn Ortwin H K , Rimmelzwaan Guus F , van Beek Ruud , Koopmans Marion , Greenwood Alex D , Czirják Gábor Á 
Published: January 2019

AbstractBackgroundInfluenza A viruses (IAVs) represent repeatedly emerging pathogens with near worldwide distribution and an unclear nonavian-host spectrum. While the natural hosts for IAV are among waterfowl species, certain mammals can be productively infected. Southern Africa is home to diverse avian and mammalian fauna for which almost no information exists on IAV dynamics.MethodsWe evaluated 111 serum samples from 14 mammalian species from Namibia for the presence of IAV-specific antibodies and tested whether host phylogeny, sociality, or diet influence viral prevalence and diversity.ResultsFree-ranging African mammals are exposed to diverse IAV subtypes. Herbivores developed antibodies against 3 different hemagglutinin (HA) subtypes, at low prevalence, while carnivores showed a higher prevalence and diversity of HA-specific antibody responses against 11 different subtypes. Host phylogeny and sociality were not significantly associated with HA antibody prevalence or subtype diversity. Both seroprevalence and HA diversity were significantly increased in carnivores regularly feeding on birds.ConclusionsThe risk of infection and transmission may be driven by diet and ecological factors that increase contact with migratory and resident waterfowl. Consequently, wild mammals, particularly those that specialize on hunting and scavenging birds, could play an important but overlooked role in influenza epizootics.
Nitric oxide and hydrogen sulfide in plants: which comes first?
Authors: Corpas Francisco J , González-Gordo Salvador , Cañas Amanda , Palma José M 
Published: January 2019

Abstract
Nitric oxide (NO) is a signal molecule regarded as being involved in myriad functions in plants under physiological, pathogenic, and adverse environmental conditions. Hydrogen sulfide (H2S) has also recently been recognized as a new gasotransmitter with a diverse range of functions similar to those of NO. Depending on their respective concentrations, both these molecules act synergistically or antagonistically as signals or damage promoters in plants. Nevertheless, available evidence shows that the complex biological connections between NO and H2S involve multiple pathways and depend on the plant organ and species, as well as on experimental conditions. Cysteine-based redox switches are prone to reversible modification; proteomic and biochemical analyses have demonstrated that certain target proteins undergo post-translational modifications such as S-nitrosation, caused by NO, and persulfidation, caused by H2S, both of which affect functionality. This review provides a comprehensive update on NO and H2S in physiological processes (seed germination, root development, stomatal movement, leaf senescence, and fruit ripening) and under adverse environmental conditions. Existing data suggest that H2S acts upstream or downstream of the NO signaling cascade, depending on processes such as stomatal closure or in response to abiotic stress, respectively.
The My Body Knows When Program Increased Intuitive Eating Characteristics in a Military Population
Authors: Cole Renee E , Meyer Stephanie A , Newman Taylor J , Kieffer Adam J , Wax Sarah G , Stote Kim , Madanat Hala 
Published: January 2019

Abstract

Introduction
The purpose of this pilot study was to assess the effectiveness of the revised My Body Knows When (MBKW) program to promote intuitive eating behaviors within a sample of a military population through an online or in-person delivery mode.

Materials and Methods
Fifty-six overweight or obese adults (70% female); military service members (20%), retirees (38%) and family (42%) participated in the 10-week MBKW program at two military installations from 2012 to 2014. Body Mass Index, Intuitive Eating Scale-2 (IES-2; 23-item) and Motivation for Eating scale (MFES; 43-item) were collected at baseline and 10-weeks. Data were stratified by sex. Descriptive data were reported as mean ± standard deviation (SD), frequency, or percentage. A paired t-test was conducted with data at baseline and 10 weeks (α = 0.05, 80% power).

Results
Participants were predominantly female (70%); mean age of 51 ± 13 years; and BMI of 34.1 ± 5.5 kg/m2. There were no demographic, MFES, or IES-2 baseline differences between groups (in-person vs. online) or location. All subjects were collapsed into one group for a pre-post MBKW implementation assessment due to small sample size despite the original intent to stratify by online and in-person grouping. At 10 weeks, the remaining 26 participants exhibited a significant improvement (mean ± SD) in BMI (−0.4 ± 0.6 kg/m2; p = 0.012), environmental/social eating score (2.7 ± 0.4 points [pts]; −0.5 pt change; p < 0.001), emotional eating score (2.2 ± 0.5 pts; −0.6 pt change; p = 0.001), unconditional permission to eat score (3.4 ± 0.4 pts; +0.3 pt change; p = 0.017), eating for physical rather than emotional eating score (3.7 ± 0.8 pts; +1.0 pt change; p < 0.001), and reliance on hunger and satiety cues score (3.6 ± 0.5 pts; +0.8 pt change; p = 0.001). High attrition rates at the 10-week follow-up assessment precluded accurate assessment of long-term intervention effects.

Conclusions
The MBKW program was associated with improved intuitive eating behaviors and with less external eating influence on behavior; however, a larger sample is required to assess the effectiveness of MBKW delivery mode. Modest weight loss was attained but testing the efficacy of the MBKW program in a large diverse sample with alternate scenarios may be worthwhile (e.g., primary prevention against weight gain, or during weight maintenance to prevent weight regain).
Dietary protein intake and kidney function decline after myocardial infarction: the Alpha Omega Cohort
Authors: Esmeijer Kevin , Geleijnse Johanna M , de Fijter Johan W , Kromhout Daan , Hoogeveen Ellen K 
Published: January 2019

Abstract

Background
Post-myocardial infarction (MI) patients have a doubled rate of kidney function decline compared with the general population. We investigated the extent to which high intake of total, animal and plant protein are risk factors for accelerated kidney function decline in older stable post-MI patients.

Methods
We analysed 2255 post-MI patients (aged 60–80 years, 80% men) of the Alpha Omega Cohort. Dietary data were collected with a biomarker-validated 203-item food frequency questionnaire. At baseline and 41 months, we estimated glomerular filtration rate based on the Chronic Kidney Disease Epidemiology Collaboration equations for serum cystatin C [estimated glomerular filtration rate (eGFRcysC)] alone and both creatinine and cystatin C (eGFRcr–cysC).

Results
Mean [standard deviation (SD)] baseline eGFRcysC and eGFRcr–cysC were 82 (20) and 79 (19) mL/min/1.73 m2. Of all patients, 16% were current smokers and 19% had diabetes. Mean (SD) total protein intake was 71 (19) g/day, of which two-thirds was animal and one-third plant protein. After multivariable adjustment, including age, sex, total energy intake, smoking, diabetes, systolic blood pressure, renin–angiotensin system blocking drugs and fat intake, each incremental total daily protein intake of 0.1 g/kg ideal body weight was associated with an additional annual eGFRcysC decline of −0.12 (95% confidence interval −0.19 to −0.04) mL/min/1.73 m2, and was similar for animal and plant protein. Patients with a daily total protein intake of ≥1.20 compared with &lt;0.80 g/kg ideal body weight had a 2-fold faster annual eGFRcysC decline of −1.60 versus −0.84 mL/min/1.73 m2. Taking eGFRcr–cysC as outcome showed similar results. Strong linear associations were confirmed by restricted cubic spline analyses.

Conclusion
A higher protein intake was significantly associated with a more rapid kidney function decline in post-MI patients.
The Incidence and Predisposing Factors of John Cunningham Virus-Induced Progressive Multifocal Leukoencephalopathy in Southern Finland: A Population-Based Study
Authors: Kartau Marge , Verkkoniemi-Ahola Auli , Paetau Anders , Palomäki Maarit , Janes Rita , Ristola Matti , Lappalainen Maija , Anttila Veli-Jukka 
Published: January 2019

Abstract

Background
The aim of this study was to assess the prevalence, incidence rate (IR), predisposing factors, survival rate, and diagnostic delay of progressive multifocal leukoencephalopathy (PML) across medical specialties. Another objective was to survey how PML diagnosis was made in the studied cases.

Methods
This is a cross-sectional retrospective observational study of PML cases across different medical specialties during 2004–2016 in the Finnish Capital Region and Southern Finland. Data were obtained from clinical records, clinical microbiology, pathology and radiology department records, and human immunodeficiency virus (HIV) quality register medical records.

Results
A total of 31 patients were diagnosed with PML. The prevalence of PML was 1.56 per 100 000 people and the IR was 0.12 per 100 000 individuals per year during 2004–2016. Hematologic malignancies (n = 19) and HIV/acquired immune deficiency syndrome (n = 5) were the most common underlying diseases, and all patients who had malignant diseases had received cancer treatment. Before PML diagnosis, 21 (67.7%) patients were treated with chemotherapy, 14 (45.2%) patients with rituximab, and 1 patient (3.2%) with natalizumab. Two patients (6.5%) had no obvious immunocompromising disease or treatment. Neither gender, age, first symptoms, previous medication, nor underlying disease influenced the survival of PML patients significantly. The 5-year survival rate was poor, at less than 10%.

Conclusions
The majority of PML patients in our study had a predisposing disease or had immunosuppressive or monoclonal antibody therapy. In the future, broader use of immunosuppressive and immunomodulatory medications may increase incidence of PML among patients with diseases unassociated with PML. Safety screening protocols for John Cunningham virus and PML are important to prevent new PML cases.
Examination of sleep health dimensions and their associations with perceived stress and health in a UK sample
Authors: Allen Sarah F , Akram Umair , Ellis Jason G 
Published: January 2019

AbstractBackgroundSleep health is a relatively new multidimensional concept, however, there is no consensus on its underlying dimensions. A previous study examined potential indicators of sleep health using an aggregated sleep health measure. However, the psychometric properties are yet to be determined. The primary aim was to assess the factor structure, reliability and validity of this measure. A secondary aim was to explore the relationships with perceived stress, and physical and mental health.MethodsA cross-sectional online survey was conducted with 257 adults from the UK aged 18–65 (78.4% female, mean age = 29.39 [SD = 11.37]). Participants completed 13 Sleep health items, the Pittsburgh Sleep Quality Inventory, Insomnia Severity Scale, Epworth Sleepiness Scale, Perceived Stress Scale and SF-12 Health Survey.ResultsThe measure exhibited good internal consistency (α = 0.785) and construct validity as determined by associations with existing measures. Principle components analysis produced four factors e; sleep quality (α = 0.818), sleep adaptability (α = 0.917), sleep wellness (α = 0.621) and daytime functioning (α = 0.582). Adaptability (β = −241) was strongest predictor of perceived stress, and daytime functioning was strongest predictor of physical (β = 0.322) and mental health (β = 0.312).ConclusionsSleep health is a multidimensional construct comprising four distinct but related dimensions. The importance of sleep health in terms of perceived stress and mental and physical health is highlighted.
Association of Gestational Diabetes Mellitus With Neonatal Respiratory Morbidity
Authors: Werner Erika F. , Romano Megan E. , Rouse Dwight J. , Sandoval Grecio , Gyamfi-Bannerman Cynthia , Blackwell Sean C. , Tita Alan T.N. , Reddy Uma M. , Jain Lucky , Saade George R. , Iams Jay D. , Clark Erin A.S. , Thorp John M. , Chien Edward K. , Peaceman Alan M. , Swamy Geeta K. , Norton Mary E. , Casey Brian M. , Caritis Steve N. , Tolosa Jorge E. , Sorokin Yoram ,   for the Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD) Maternal-Fetal Medicine Units Network (MFMU)*
Published: January 2019

OBJECTIVE:
To assess neonatal respiratory morbidity in pregnancies with and without gestational diabetes mellitus (GDM) at imminent risk of late preterm delivery in a modern U.S. cohort.

METHODS:
Secondary analysis of a randomized placebo-controlled trial in which women with singleton pregnancies at high risk for delivery between 34 0/7 and 36 5/7 weeks of gestation were allocated to betamethasone or placebo. The primary outcome for the trial and this secondary analysis was a composite outcome of neonatal respiratory morbidity in the first 72 hours of life. Secondary outcomes included neonatal severe respiratory complications, neonatal intensive care unit (NICU) admission greater than or equal to 3 days, and hyperbilirubinemia. We examined associations between neonatal morbidities and GDM status after adjustment for baseline differences and study group allocation using modified Poisson regression. Models incorporating a product interaction term between GDM status and treatment arm (betamethasone or placebo) were also evaluated.

RESULTS:
Of the 2,831 women enrolled in the trial, 306 (10.8%) had GDM. Women with GDM were significantly older and were more likely to be parous and to have hypertensive disorders of pregnancy than those without GDM, but they were similar regarding race, gestational age at randomization (35.6 weeks) and at delivery (36.1 weeks), and study group assignment. Neonates born to women with GDM were no more likely to meet the primary outcome than those born to women without GDM, even after adjusting for differences in age, parity, and hypertensive disorders of pregnancy (12.1% vs 13.1%, adjusted RR 0.84; 95% CI 0.61–1.17), nor were they more likely to have severe respiratory complications or prolonged NICU admission.

CONCLUSION:
Maternal GDM is not associated with increased neonatal respiratory morbidity in this study population who were at high risk for late preterm birth.