bluuebunny/crossref_metadata_2025_split huggingface dataset:
All-optical electrophysiology reveals excitation, inhibition, and neuromodulation in cortical layer 1
Authors: Fan Linlin Z. , Kheifets Simon , Böhm Urs L. , Piatkevich Kiryl D. , Wu Hao , Parot Vicente , Xie Michael E. , Boyden Edward S. , Takesian Anne E. , Cohen Adam E. 
Published: April 2019

AbstractThe stability of neural dynamics arises through a tight coupling of excitatory (E) and inhibitory (I) signals. Genetically encoded voltage indicators (GEVIs) can report both spikes and subthreshold dynamics in vivo, but voltage only reveals the combined effects of E and I synaptic inputs, not their separate contributions individually. Here we combine optical recording of membrane voltage with simultaneous optogenetic manipulation to probe E and I individually in barrel cortex Layer 1 (L1) neurons in awake mice. Our studies reveal how the L1 microcircuit integrates thalamocortical excitation, lateral inhibition and top-down neuromodulatory inputs. We develop a simple computational model of the L1 microcircuit which captures the main features of our data. Together, these results suggest a model for computation in L1 interneurons consistent with their hypothesized role in attentional gating of the underlying cortex. Our results demonstrate that all-optical electrophysiology can reveal basic principles of neural circuit function in vivo.One Sentence SummaryAll-optical electrophysiology revealed the function in awake mice of an inhibitory microcircuit in barrel cortex Layer 1.
pH-responsive nano-vaccines as antigen and adjuvant carrier that improve vaccine immunogenicity
Authors: Freeman Daniel , Smith John 
Published: April 2019

AbstractHere, we report a novel method to establish an effective antigen and immunoagonist co-vector to solve the problems of low antigenic endocytosis efficiency, low immunological activity and easy degradation of antigen by antigen presenting cells. Mesoporous silica was selected as the nucleus. After loading the model antigen chicken egg albumin (OVA), the metal organic framework (MOF) formed by Eu 3+ and guanine mononucleotide (GMP) was coated on the mesoporous silicon surface. The immunostimulant CpG nucleic acid is adsorbed on the surface to construct a co-carrier system of pH-sensitive antigen and immunostimulant. The antigen loading rate of mesoporous silicon was 20%, and the protein release amount reached 55% after incubation for 24 h under acidic conditions. Transmission electron microscopy showed that the conjugated polymer was uniformly coated on the surface of the material; It was found that the adsorption capacity of the carrier for CpG nucleic acid was 8 ×10−6 mol per gram of carrier−adsorbing nucleic acid; MTT results showed that the vector had low toxicity.
Empathy for service: benefits, unintended consequences, and future research agenda
Authors: Bove Liliana L. 
Published: April 2019

PurposeEmpathy is a core characteristic of helping and caring interactions and thus is fundamental to service. Yet, to date, service marketing literature has focused on a restricted view of the value of empathy as it leads to improved service quality perceptions and successful sales outcomes. The aim of this paper is to provide a review of the empathy literature and the dispositional and situational factors affecting it, so as to further explore its potential benefits and limitations for service.Design/methodology/approachA summative review of the empathy literature uncovers cause–effect relationships and their potential boundary conditions. Theoretical propositions set an agenda for future research on empathy for service that breaks new ground.FindingsEmpathy can reduce anti-social, revenge, discrimination and unethical behaviors in service settings. It can also improve value-in-context experiences for users of service innovations. Notwithstanding its potential benefits, empathy can diminish the objectivity and performance of service providers when experienced at extreme levels. Empathy can also serve as an ingratiation influence tactic and can be detrimental to the target in embarrassing service contexts.Originality/valueThis paper suggests propositions for future research to advance theory and managerial practice on the use of empathy to improve service outcomes for interacting actors. It also alludes to the potential dark side of empathy when experienced at excessive levels or when used to manipulate.
Development of a consumer experience reporting questionnaire for residential aged care homes in Australia
Authors: Wells Yvonne , Fetherstonhaugh Deirdre , Solly Kane Norman 
Published: April 2019

ObjectiveTo develop and pilot a short interview tool to be implemented by the Australian Aged Care Quality Agency (AACQA) in residential aged care, to inform prospective consumers about the quality of care and services received—the consumer experience report (CER).MethodsTwenty‐four questions addressing the four Aged Care Quality Standards were piloted with 140 residents and 48 representatives (including 27 resident‐representative pairs). A method for approximating random selection was also trialled. Fifty‐two residents were interviewed twice, one week apart, and each question's usability was rated by 11 surveyors and two interpreters. Selection criteria for questions included consumer preference, test‐retest reliability, resident‐representative agreement, usability and low missing data.ResultsNineteen questions performed well on missing data, 16 on test‐retest, 17 on resident‐representative agreement and 12 on usability. Ten quantitative and two open‐ended questions were selected.ConclusionsThe CER questions proved suitable to collect data systematically on consumer experience in residential aged care. AACQA (now the Aged Care Quality and Safety Commission) uses the CER interview to support their audit process, and aggregated results are published online.
Letter: nonselective beta‐blockers, endoscopic therapy and portal vein thrombosis in cirrhosis. Authors' reply
Authors: Nery Filipe , Valla Dominique 
Published: April 2019

Linked ContentThis article is linked to Nery et al and Bosch and Berzigotti papers. To view these articles, visit https://doi.org/10.1111/apt.15137 and https://doi.org/10.1111/apt.15236.
Single centre experience of bilateral gracilis flap perineal reconstruction following extra‐levator abdominoperineal excision
Authors: Coelho J. A. J. , McDermott F. D. , Cameron O. , Smart N. J. , Watts A. M. , Daniels I. R. 
Published: April 2019

AbstractAimA variety of tissue flaps have been described for the closure of perineal wounds following abdominoperineal excision of the rectum (APE) or exenteration for locally advanced/recurrent rectal cancer and salvage surgery for anal cancer. The aim of this study was to demonstrate the utility of the bilateral pedicled gracilis muscle flaps (BPGMFs) as a reconstruction option in these patients. This is of particular benefit when using a laparoscopic approach for the abdominal component of the operation, avoiding disruption of the abdominal wall and risk of herniation with other reconstruction options, e.g. vertical rectus abdominis myocutaneous flaps.MethodThis is a retrospective single centre case series of patients who underwent reconstruction of perineal defects using BPGMFs using a novel weave technique, from January 2008 to August 2017.ResultsThere were 25 patients (16 female), with a median follow‐up of 19 months (3–102). The indications for BPGMFs were cancer resection (21) and perineal hernia (4). The median length of stay was 14 days (6–60). All‐cause mortality was 36% within the follow‐up period. A healed perineal wound was achieved in 72% of patients within 30 days (84% of patients received neoadjuvant chemoradiotherapy). The overall donor site complication rate was 20% (including infection, dehiscence, numbness, haematoma and seroma) and 28% for the perineal site (including infection, dehiscence and prolapse).ConclusionsBPGMFs provide an important option for reconstruction of the perineum particularly with a minimally invasive approach or with two stomas.
Antarctica: The final frontier for marine biological invasions
Authors: McCarthy Arlie H. , Peck Lloyd S. , Hughes Kevin A. , Aldridge David C. 
Published: April 2019

AbstractAntarctica is experiencing significant ecological and environmental change, which may facilitate the establishment of non‐native marine species. Non‐native marine species will interact with other anthropogenic stressors affecting Antarctic ecosystems, such as climate change (warming, ocean acidification) and pollution, with irreversible ramifications for biodiversity and ecosystem services. We review current knowledge of non‐native marine species in the Antarctic region, the physical and physiological factors that resist establishment of non‐native marine species, changes to resistance under climate change, the role of legislation in limiting marine introductions, and the effect of increasing human activity on vectors and pathways of introduction. Evidence of non‐native marine species is limited: just four marine non‐native and one cryptogenic species that were likely introduced anthropogenically have been reported freely living in Antarctic or sub‐Antarctic waters, but no established populations have been reported; an additional six species have been observed in pathways to Antarctica that are potentially at risk of becoming invasive. We present estimates of the intensity of ship activity across fishing, tourism and research sectors: there may be approximately 180 vessels and 500+ voyages in Antarctic waters annually. However, these estimates are necessarily speculative because relevant data are scarce. To facilitate well‐informed policy and management, we make recommendations for future research into the likelihood of marine biological invasions in the Antarctic region.
Facile synthesis of α‐Si3N4 nanoneedles and their photoluminescence properties
Authors: Shen Xiulin , Liu Haitao , Zhang Xianmei , Huang Zhaohui , Liu Yangai , Fang Minghao , Wu Xiaowen , Min Xin 
Published: April 2019

AbstractNeedle‐like α‐Si3N4 with unique structures has potential application in both field emission devices and tips for atomic force microscopes. Single‐crystalline, α‐Si3N4 nanoneedles have been prepared by using an improved chemical vapor deposition (CVD) method at 1200°C for 3 hours. The phases, chemical composition, and microstructure of the as‐prepared products were determined by X‐ray diffraction (XRD), field emission scanning electron microscopy (FESEM), and transmission electron microscopy (TEM) equipped with EDS. The as‐synthesized products show a needle‐like morphology with hundreds of micrometers in length and nanometer‐featured tips. The nanoneedles show a α‐Si3N4@SiOx core‐shell heterostructure as characterized by TEM, with single‐crystalline α‐Si3N4 core and an insulating amorphous silicon oxide shell. The growth of α‐Si3N4 nanoneedles corresponds to vapor‐liquid‐solid cap‐growth and base‐growth mechanism. Photoluminescence (PL) properties of the products were also characterized. An obvious emission peak at 400 nm under 254 nm UV excitation was observed. The nanoneedle morphology and photoluminescence properties of the products have potentials to be used in future optoelectronic nanodevices.
Bisphenol A promotes the proliferation of leiomyoma cells by GPR30‐EGFR signaling pathway
Authors: Li Zemin , Lu Qing , Ding Bo , Xu Jingyun , Shen Yang 
Published: April 2019

AbstractAimTo study the molecular mechanism of G protein‐coupled receptor 30‐epidermal growth factor receptor (GPR30‐EGFR) signaling pathway on the proliferation of leiomyoma cells exposed with bisphenol A.MethodsPrimary cultures and subcultures of human uterine leiomyoma (UL) cells. The expressions of messenger RNA and proteins of GPR30 and EGFR in 15 leiomyoma tissue specimens and all groups were detected by real‐time quantitative polymerase chain reaction assay and Western blot assay. The protein of mitogen‐activated protein kinases (MAPK)/extracellular signal–regulated kinases (ERK)/c‐fos signaling pathway members was detected by Western blot assay.ResultsBisphenol A promoted the growth of UL cells and the expressions of GPR30, EGFR, c‐fos and p‐ERK1/2.ConclusionBisphenol A was found to be a promoter specifically to proliferate the human UL cells by activating the transcription and translation of GPR30‐EGFR and MAPK/ERK/c‐fos signaling pathway members.
Inhibition of aerobic glycolysis enhances the anti‐tumor efficacy of Zoptarelin Doxorubicin in triple‐negative breast cancer cells
Authors: Gründker Carsten , Wokoun Ulrike , Hellriegel Martin , Emons Günter 
Published: April 2019

AbstractAimA characteristic of cancer cells including triple‐negative breast cancers (TNBC) is an increased aerobic glycolysis for ATP production representing a selective therapeutic target. More than 70% of TNBC express gonadotropin‐releasing hormone receptors (GnRH‐R). These receptors can be used for targeted chemotherapy with cytotoxic GnRH agonists such as Zoptarelin Doxorubicin, in which doxorubicin is covalently linked to [D‐Lys6]GnRH. In this study, we have analyzed whether inhibition of aerobic glycolysis can enhance the antitumor efficacy of GnRH‐R‐targeted chemotherapy using Zoptarelin Doxorubicin.MethodsTriple‐negative breast cancers cell lines MDA‐MB‐231 and HCC1806 were treated with Zoptarelin Doxorubicin, glycolysis inhibitor 2‐deoxy‐D‐glucose (2DG) or the combination of both agents. Cell viability was measured using Alamar blue. Induction of apoptosis was quantified by measurement of loss of mitochondrial membrane potential. In vivo experiments were performed using nude mice bearing xenografted MDA‐MB‐231 tumors.ResultsTreatment of TNBC cells with Zoptarelin Doxorubicin or with 2DG resulted in a significant decrease of cell viability and a significant increase of apoptosis. Treatment with Zoptarelin Doxorubicin in combination with 2DG resulted in significantly reduced viability and enhanced apoptosis compared with single‐agent treatments. Combinational index (CI) analysis revealed the co‐treatment effect as a synergistic. The antitumor effects of Zoptarelin Doxorubicin or 2DG were confirmed in nude mice. The tumor reducing effects of Zoptarelin Doxorubicin were enhanced by combination with 2DG.ConclusionThe glycolytic phenotype of TNBC can be used to improve antitumor therapies. Co‐treatment of Zoptarelin Doxorubicin with glycolysis inhibitor 2DG might be a suitable therapeutic option for GnRH receptor‐positive TNBC.
Feasibility of integrating a dental hygienist into an inpatient medical team for patients with diabetes mellitus
Authors: Simon Lisa , Tobey Matthew , Wilson Marlena 
Published: April 2019

AbstractPatients who are hospitalized face a high risk of poor oral health and experience a decline in oral hygiene over the course of hospitalization. Due to the documented links between diabetes and periodontal disease as well as the prevalence of diabetes in the hospitalized population, patients with type 2 diabetes mellitus were chosen as the population of interest for a pilot program of dental hygienist‐led care provision in the hospital. Inclusion criteria were established by agreement of a dentist and physician. Nursing and medical staff were provided with multiple opportunities to learn about the dental hygienist's role throughout the pilot. The dental hygienist utilized a fully mobile dental unit and bedside suction unit and followed all standard infection control procedures. This intervention identified a number of suboptimal oral health practices among patients and was perceived positively by patients and providers. No adverse events occurred.
Pulse Oximeter for Low SpO2 Level Detection Using Discrete
Time Signal Processing Algorithm
Authors: Pandey Sumit , Tan Cher Ming , Chen Hsiao-Wen , Xie Yao En , Tung Jung Hua , Kau Yu-Chuan , Liao Chia-Chih 
Published: April 2019

Abstract
Oximeter is an important clinical device used for measuring peripheral capillary oxygen saturation (SpO2) in blood and hence accurate results are needed in order to help physicians predict clinical problems in the initial stage(s) of liver or kidney diagnosis. Different issues associated with the accuracy of SpO2 and heart rate measurement accuracy are studied in this work. With the understanding of these issues, a new SpO2 monitoring system is proposed that comprises of a better detection method, novel discrete time signal processing (DTSP) algorithm, and a custom-made oximeter probe head. The proposed SpO2 measurement system is capable of determining low levels of SpO2 present in human blood and produce the results in a short time that enable real-time monitoring of a patient SpO2. It can also distinguish low level of SpO2 against background noise.
Quality improvement initiative using transcutaneous bilirubin nomogram to decrease serum bilirubin sampling in low-risk babies
Authors: Shah Muhammad Hussain , Ariff Shabina , Ali Syed Rehan , Chaudhry Rayaan Asad , Lakhdir Maryam Pyar Ali , Qaiser Fatima , Demas Simon , Hussain Ali Shabbir 
Published: April 2019

BackgroundScreening for neonatal hyperbilirubinaemia in the postnatal ward has traditionally been performed using serum bilirubin sampling, but this has significant drawbacks such as risk of infection and slower reporting time.ObjectiveWe aimed to assess the impact of introducing transcutaneous bilirubin (TcBR) testing using TcBR nomogram on the number of serum bilirubin samples sent.MethodsA before-and-after study was performed following the introduction of a protocol integrating the use of the Dragger JM-105 transcutaneous bilirubinometer in the postnatal ward. Only babies born at ≥37 weeks of gestation, weighing ≥2500 g who presented with jaundice after the first 24 hours and within the first 7 days of life were included in the study. The number of total serum bilirubin samples (TSBRs) sent were compared for the 6-month periods before and after (a total of 12 months) implementation of the new protocol.ResultsIn the pre-implementation phase, a total of 882 (49%) out of 1815 babies had at least one serum bilirubin sample taken as opposed to a total of 236 (17%) out of 1394 babies in the post-implementation phase. The odds of performing TSBRs at least one time among babies in post-implementation phase were 79% lower than in pre-implementation phase (OR 0.21, 95% CI 0.18 to 0.25). We also estimated a significant cost saving of approximately US$1800 over a period of 6 monthsConclusionTcBR testing used in conjunction with our proposed nomogram significantly reduces the need for serum bilirubin sampling.
The Belle II experiment: Status and physics prospects
Authors: Shiu Jing-Ge 
Published: April 2019

The Belle II experiment at SuperKEKB [Formula: see text] collider, Japan, is aiming at exploring flavor physics in high intensity frontier. The target instantaneous luminosity of SuperKEKB is at [Formula: see text], which is 40 times higher than that at KEKB. The renovated Belle II detectors around the interaction point are expected to collect [Formula: see text] data within 7 years. This gives high data statistics for precision measurements and probing new physics in rare decay studies. The Belle II experiment has successfully recorded the first collision events at SuperKEKB in April 2018, and is ready for physics data taking. This paper will cover the current status of Belle II and highlight its prospects on flavor physics.
Inquiry Based Learning Module to Empower Cooperation Skills
Authors: Anjani Dewi , Maridi Maridi , Suciati Suciati 
Published: April 2019

The education in the 21st century focuses on knowledge and encourages students to generate information and encourage developing new skills. The framework of 21st century learning skill is communication, collaboration, critical thinking, creativity and innovation. Cooperation skills in the world of education is an important thing to be done in learning. Therefore, a solution is needed to optimize student empower cooperation skills process by using the teaching material in the form of module. The objective of this research is to know the effectiveness of inquiry based learning module to empower cooperation skills. This research was conducted in one of high school in Surakarta, Indonesia. The research method is quasi experiment, used pretest and posttest design by using two randomly selected classes those were experimental class used an inquiry based learning module and control one used a modules made by biology teachers in Surakarta. The results showed that there were significant differences of learning outcomes between the control and the experimental classes based on the independent samples t-test test results, also seen with the n-gain scores that obtained at 0.78 (middle) in the control class and 0.87 (high) in the experimental class. As the study concluded, using inquiry based learning module in the learning process in effective to empower cooperation skills.
Development of an inexpensive data logger for solar water heating system regulators
Authors: Wassima Ait ahmed , Mohammed Aggour , Badr Ouhammou 
Published: April 2019

<p>Each solar thermal plant can provide different parameters to be treated and evaluated, mostly temperature measurements, pressure specifications, heat transfer values. This data collection can be quite expensive taking into account the important number of sensors and data loggers and their specifications. Hence to assure flexibility in the data collection at a low cost, many hardware and software specifications can be adopted. Here we built an inexpensive data logger based on the Arduino board and IDE to collect and store incoming data from Resol DeltaSol regulators integrated in different solar water heating systems. The objective of this study is to allow massive synchronized measurements with high resolution to be stored into an SD card through the Arduino platform. The measurements are given by multiple sensors (temperature, pump speed, relay states) connected to the regulator. The results from the tests of the plant connected to the data logger are provided later on, alongside the commercial aspects of the built data logging device. After a month of daily measurements, the data logger showed an outstanding performance with an extreme low cost compared to industrial datalogger. </p>
Transcutaneous Oxygen Tension Measurement in Patients with Chronic Arterial Obstructive Disease: Reliability and Long-Term variability of the Method
Authors: Melillo Elio , Catapano Giosue' , Ferrari Mauro , Pedrinelli Roberto 
Published: April 2019

Although transcutaneous oxygen tension (TcpO2) measurement may be useful for assessing changes in regional perfusion induced over time by drug or surgical treatment in patients with chronic arterial obstructive disease (CAOD), the reliability of the method over a long-term period is not know To approach this problem, the authors evaluated retrospectively the behavior of TcpO2 measurement over time in patients with CAOD. To eliminate confounding influences due to the concomitant vascular disease at the limb level, data analysis was performed on TcpO2 measured at the right infraclavicular position. The median length of follow-up ranged from twenty days in 34 patients to 832 days in 3 patients (n=2 and n=10 individual sequential replications respectively). Initial and final TcpO 2 values did not differ significantly even at the longest follow-up term, which indicates that the parameter is constant over time. The intrapatient variation coefficient of TcpO2 (calculated over at least three individual replications) ranged between an average of 11% to 16.2%. The corresponding interpatient variation fluctuated between 15.5% and 33.4%, a variability explained to some extent by sex-related influ ences, but, at least in the range of this sample, not by age, arterial oxygen levels, or disease status. Thus TcpO2 levels per se are stable, implying that TcpO2 measurement has the potential to record consistent changes caused by specific therapeutic interventions or the clinical evolution of patients with CAOD. However, the intrapatient and interpa tient variability of the method has to be taken into account when TcpO2 is used for the follow-up and the physiopathologic study of patients with CAOD.