bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessment of Common Soybean-Infecting Viruses in Ohio, USA, Through Multi-site Sampling and High-Throughput Sequencing
Authors: Han Junping , Domier Leslie L. , Cassone Bryan J. , Dorrance Anne , Qu Feng 
Published: July 2018

Multi-site sampling was conducted during 2011 and 2012 to assess the scope of virus disease problems of soybean in Ohio, USA. A total of 259 samples were collected from 80 soybean fields distributed in 42 Ohio counties, accounting for more than 90% of major soybean-growing counties in Ohio. A high-throughput RNA-Seq approach was adopted to identify all viruses in the samples that share sufficient sequence similarities with known plant viruses. To minimize sequencing costs, total RNA extracted from up to 20 samples were first pooled to make up regional pools, resulting in eight regional pools per year in both 2011 and 2012. These regional pools were further pooled into two yearly master pools of RNA, and sequenced using the Illumina's HiSeq2000 platform. Bioinformatic analyses of sequence reads led to the identification of signature sequences of nine different viruses. The originating locations of these viruses were then mapped with PCR or RT-PCR. This study confirmed the widespread distribution of Bean pod mottle virus, Soybean vein necrosis virus, Tobacco ringspot virus, and Tobacco streak virus in Ohio. It additionally revealed occasional association of Alfalfa mosaic virus, Bean yellow mosaic virus, Clover yellow vein virus, Soybean mosaic virus, and Soybean Putnam virus with Ohio soybean. This is the first statewide survey of soybean viruses in Ohio, and provides the much-needed baseline information for management of virus diseases of soybean. Accepted for publication 20 May 2016. Published 10 June 2016.
Socioenvironmental stressors encountered during spaceflight partially affect the murine TCR‐β repertoire and increase its self‐reactivity
Authors: Fonte Coralie , Kaminski Sandra , Vanet Anne , Lanfumey Laurence , Cohen-Salmon Charles , Ghislin Stéphanie , Frippiat Jean-Pol 
Published: July 2018

ABSTRACTSpaceflights are known to affect the immune system. In a previous study, we demonstrated that hypergravity exposure during murine development modified 85% of the T‐cell receptor (TCR)‐β repertoire. In this study, we investigated whether socioenvironmental stressors encountered during space missions affect T lymphopoiesis and the TCR‐β repertoire. To address this question, pregnant mice were subjected throughout gestation to chronic unpredictable mild stressors (CUMS), a model used to mimic socioenvironmental stresses encountered during space missions. Then, newborn T lymphopoiesis and the TCR‐β repertoire were studied by flow cytometry and high‐throughput sequencing, respectively. No change in thymocyte maturation or TCR expression were noted. TCR‐β repertoire analysis revealed that 75% of neonate TCR‐β sequences resulted from the expression of 3 variable (V)β segments and that this core repertoire was not affected by CUMS. However, the minor repertoire, representing 25% of the global repertoire, was sensitive to CUMS exposure. We also showed that the variable (diversity) joining [V(D)J] recombination process was unlikely to be affected. Finally, we noted that the CUMS neonatal minor repertoire was more self‐reactive than the one of control pups. These findings show that socioenvironmental stressors such as those encountered during space missions affect a fraction (25%) of the TCR‐β repertoire and that these stressors could increase self‐reactivity.—Fonte, C., Kaminski, S., Vanet, A., Lanfumey, L., Cohen‐Salmon, C., Ghislin, S., Frippiat, J.‐P. Socioenvironmental stressors encountered during spaceflight partially affect the murine TCR‐β repertoire and increase its self‐reactivity. FASEB J. 33, 896–908 (2019). www.fasebj.org
Early Predictors of Long-term Outcomes in Childhood Glaucoma
Authors: Chang Ta C. , Cavuoto Kara M. , Grajewski Alana L. , Hodapp Elizabeth A. , Vanner Elizabeth A. 
Published: July 2018

Purpose:
A data-based staging system for childhood glaucoma is likely to improve the prediction of visual acuity and intraocular pressure (IOP) control outcomes. We investigated early clinical factors associated with poor long-term visual, and IOP control outcomes in early-onset glaucoma as the initial steps to constructing a severity staging system.

Design:
Statistical modeling of retrospective case series data.

Participants:
Glaucoma patients younger than 3 years of age who presented to Bascom Palmer Eye Institute between 1990 and 2010 with at least 5 years of follow up.

Methods:
Statistical modeling of retrospective case series data from first, second, third, 3-year, 5-year, and final visits.

Main Outcome Measures:
Association of early clinical characteristics to final IOP-control outcomes and visual acuities.

Results:
A total of 26 eyes of 15 children were included. Nine of 15 (60%) of patients were male. Mean age at initial presentation: 9.98±10.55 months. Mean duration between initial and final visits: 11.13±3.55 years. By the third visit (mean 6.69 mo after presentation), presence of nystagmus, anterior segment dysgenesis (ASD) or having failed angle surgery increased the final LogMAR visual acuity by 0.76 (P=0.0516), 0.64 (P=0.0618), and 0.58 (P=0.0159), respectively. At year 3, failed amblyopia therapy, failed angle surgery, nystagmus, media opacity (MO), or ASD increased the final LogMAR by 1.30, 1.34, 1.21, 0.85, 0.64, respectively (all P<0.02). Failed angle surgery or MO increased the chance of uncontrolled IOP at the final visit (proportional odds ratio of 6.77 and 12.88, respectively).

Conclusions:
In this pilot study of a modest size cohort, the presence of nystagmus, ASD or failed angle surgery early in the course of infantile-onset glaucoma management predicted poor final visual outcome, whereas failed angle surgery and presence of MO predicted poor final IOP control. These predictors of poor outcomes will serve as the initial steps in constructing a severity staging system.
Outcomes of Silicone Ahmed Glaucoma Valve Implantation in Refractory Pediatric Glaucoma
Authors: Senthil Sirisha , Turaga Kiranmaye , Mohammed Hasnat A. , Krishnamurthy Rashmi , Badakere Swathi , Dikshit Siddharth , Choudhari Nikhil , Mandal Anil K. , Garudadri Chandra S. 
Published: July 2018

Purpose:
The purpose of this study was to report the outcomes of Silicone Ahmed Glaucoma Valve (AGV) implantation in the management of refractory pediatric glaucoma.

Methods:
Between 2007 and 2015, 76 eyes of 64 children aged 16 years or younger underwent AGV implantation. We included 65 eyes of 53 children with follow up ≥6 months; 24 eyes had primary congenital glaucoma (PCG) and 41 eyes had secondary pediatric glaucoma (SPG). Success was defined as IOP>5 and ≤21 mm Hg with or without topical antiglaucoma medications; considered failure, when repeat glaucoma surgery was need or loss of light perception. Primary outcome measure was success of AGV in refractory pediatric glaucomas and secondary outcome measure was comparison of outcomes in PCG and SPG.

Results:
The median age at AGV implantation was 3 years (interquartile range, 2, 12), and median follow up was 27 months (15, 39). The overall cumulative success probability was 88% [95% confidence interval (CI), 76%-94%] at 1 year and was maintained up to 4 years. The success probability in PCG was 91% (80.8%-100%), and SPG was 83% (72%-96%) at 1 year and 4 years (P=0.49). Among the preoperative factors, number of previous intraocular surgeries (hazard ratio of 2.24; 95% CI, 1.14-4.37, was significantly associated with failure; P=0.01). Tube-related complications (16%) were similar in both the groups. One eye in each group had sight-threatening complication.

Conclusions:
AGV implantation had good success rate in refractory pediatric glaucoma. The success rates were similar in PCG and SPG as were the complications. Number of prior intraocular surgeries was a risk factor for failure.
Twenty-five years of pain education research—what have we learned? Findings from a comprehensive scoping review of research into pre-registration pain education for health professionals
Authors: Thompson Kate , Johnson Mark I. , Milligan James , Briggs Michelle 
Published: July 2018

Abstract
The International Association for the Study of Pain (IASP) has designated 2018 as the global year for excellence in pain education. Despite advances in pain research, there remains an inadequate understanding and implementation of pain education that health professionals obtain in training before professional registration, licensure, or certification. This article reports on a synthesis of pain education research that has been conducted in this period of health professionals' training. A scoping review framework by Arksey and O'Malley was used to guide a search of medical and education databases for records that have examined or evaluated pain education. Fifty-six reports were identified representing 16 professions across 29 countries, published between 1992 and 2017. A descriptive account of the reports is provided, which includes a timeline, geography, methods of evaluating, and main purpose of the research. A narrative synthesis was undertaken to summarise and explain the results and main findings from reports of studies included in this review. Further to this, a concept analysis was conducted to identify and map key concepts that can be used by stakeholders to develop or evaluate future pain education. Future directions for research are proposed, which includes factors that are repeatedly reported to be important in advancing pain education and should underpin the campaign for environments that promote excellence in pain practice as the norm in health care.
Student Perceptions Regarding Collaborative Intraprofessional Nursing Education
Authors: George Tracy P. , Murphy Pamela Ford , DeCristofaro Claire , Hucks J. Marty 
Published: July 2018

Background
Teamwork is an important factor in the provision of high-quality health care. There is a lack of research on collaboration between nursing students at different program levels.

Purpose
The purpose of this project was to determine student perceptions about collaborative learning activities between prelicensure BSN and MSN students.

Methods
Community assessments by BSN students identified health needs and issues for 6 underserved populations. Online MSN students used these assessments to create low-literacy patient education pamphlets. In turn, BSN students provided educational sessions at community sites using the pamphlets. Both groups completed presurveys and postsurveys assessing their perceptions of collaborative learning.

Results
There were statistically significant differences between the respondent groups for 3 survey questions about how this project helped prepare them for future practice and professional collaboration. Eight qualitative themes were identified.

Conclusion
Although desiring more face-to-face interaction between groups, students reported that collaboration was important.
Accurate Prediction of Congenital Heart Surgical Length of Stay Incorporating a Procedure-Based Categorical Variable*
Authors: Alexander Peta M. A. , DiOrio Matthew , Andren Kristofer , Gauvreau Kimberlee , Mistry Kshitij P. , Mathieu Derek , Wright Jonathan , Allan Catherine K. , Nathan Meena , Mayer John E. , Thiagarajan Ravi R. , Bergersen Lisa 
Published: July 2018

Objectives:
There is increasing demand for the limited resource of Cardiac ICU care. In this setting, there is an expectation to optimize hospital resource use without restricting care delivery. We developed methodology to predict extended cardiac ICU length of stay following surgery for congenital heart disease.

Design:
Retrospective analysis by multivariable logistic regression of important predictive factors for outcome of postoperative ICU length of stay greater than 7 days.

Setting:
Cardiac ICU at Boston Children’s Hospital, a large, pediatric cardiac surgical referral center.

Patients:
All patients undergoing congenital heart surgery at Boston Children’s Hospital from January 1, 2010, to December 31, 2015.

Interventions:
No study interventions.

Measurements and Main Results:
The patient population was identified. Clinical variables and Congenital Heart Surgical Stay categories were recorded based on surgical intervention performed. A model was built to predict the outcome postoperative ICU length of stay greater than 7 days at the time of surgical intervention. The development cohort included 4,029 cases categorized into five Congenital Heart Surgical Stay categories with a C statistic of 0.78 for the outcome ICU length of stay greater than 7 days. Explanatory value increased with inclusion of patient preoperative status as determined by age, ventilator dependence, and admission status (C statistic = 0.84). A second model was optimized with inclusion of intraoperative factors available at the time of postoperative ICU admission, including cardiopulmonary bypass time and chest left open (C statistic 0.87). Each model was tested in a validation cohort (n = 1,008) with equivalent C statistics.

Conclusions:
Using a model comprised of basic patient characteristics, we developed a robust prediction tool for patients who will remain in the ICU longer than 7 days after cardiac surgery, at the time of postoperative ICU admission. This model may assist in patient counseling, case scheduling, and capacity management. Further examination in external settings is needed to establish generalizability.
Content validation of a Critical Appraisal Tool for Reviewing Analgesia Studies (CATRAS) involving subjects incapable of self-reporting pain
Authors: Warne Leon N. , Schug Stephan A. , Beths Thierry , Brondani Juliana T. , Carter Jennifer E. , Lascelles B. Duncan X. , Raisis Anthea L. , Robertson Sheilah A. , Steagall Paulo V.M. , Taylor Polly M. , Whittem Ted , Bauquier Sébastien H. 
Published: July 2018

AbstractIntroduction:This article reports the content validation of a Critical Appraisal Tool designed to Review the quality of Analgesia Studies (CATRAS) involving subjects incapable of self-reporting pain and provide guidance as to the strengths and weakness of findings. The CATRAS quality items encompass 3 domains: level of evidence, methodological soundness, and grading of the pain assessment tool.Objectives:To validate a critical appraisal tool for reviewing analgesia studies involving subjects incapable of self-reporting pain.Methods:Content validation was achieved using Delphi methodology through panel consensus. A panel of 6 experts reviewed the CATRAS in 3 rounds and quantitatively rated the relevance of the instrument and each of its quality items to their respective domains.Results:Content validation was achieved for each item of the CATRAS and the tool as a whole. Item-level content validity index and kappa coefficient were at least greater than 0.83 and 0.81, respectively, for all items except for one item in domain 2 that was later removed. Scale-level content validity index was 97% (excellent content validity).Conclusions:This 67-item critical appraisal tool may enable critical and quantitative assessment of the quality of individual analgesia trials involving subjects incapable of self-reporting pain for use in systematic reviews and meta-analysis studies.
Dual-Energy Computed Tomography Applications in Neurointervention
Authors: Wolman Dylan N. , Patel Bhavik P. , Wintermark Max , Heit Jeremy J. 
Published: July 2018

Abstract
Dual-energy computed tomography (CT) combines the high spatial resolution of standard CT with the ability to improve contrast resolution, reduce artifact, and separate materials of different atomic weights and energy-based attenuation through postprocessing. We review the underlying physical principles and applications of dual-energy CT within the context of patients undergoing preprocedural and postprocedural evaluation for neurointerventional therapies. The broad imaging categories of cerebral ischemia and hemorrhage, head and neck angiography, and the spine are reviewed.
Introduction of Meglumine Diatrizoate into the Middle Cranial Fossa: A Rare Fatal Complication of Temporomandibular Joint Arthrography
Authors: Li Wei , Wei Lili , Wang Fang , Cheng Yong , Li Bo 
Published: July 2018

Abstract
The authors here report a rare fatal complication of temporomandibular joint (TMJ) arthrography. A 57-year-old woman suddenly exhibited spasm and dizziness during TMJ arthrography. A multislice CT scanning of head demonstrated a defect in the roof of the glenoid fossa and unanticipated introduction of meglumine diatrizoate into the middle cranial fossa, which should account for neurotoxic symptoms of the patient and could be fatal if not appropriately treated in time. As TMJ puncture is widely performed in clinical practice and generally considered a safe technique, this case might serve as a reminder of the potential risk of the anatomical variation—a defect in the roof of the glenoid fossa—to TMJ clinical practitioners.
The Nonsurgical Management of Orbital Dystopia Using Refractive Lenses and Prosthetic Shells
Authors: Joganathan Varajini , Terry Patsy , Wall Steve , Norris Jonathan H. 
Published: July 2018

Abstract
Surgical rehabilitation of orbital dystopia can be challenging. The authors demonstrate the effective use of spectacle lenses to visually correct misalignments of the globe and the orbit. Presented is a retrospective review of 4 patients undergoing aesthetic rehabilitation through use of spectacle lenses and in a number patients a cosmetic shell.
Two patients with neurofibromatosis presented with inferior dystopia of the globe and orbit. A base-down prismatic lens applied to the spectacles in conjunction with a prosthetic shell successfully visually corrected the facial asymmetry and improved patients’ aesthetic appearance. One patient with a history of traumatic retinal detachment, who did not want any surgical intervention, a “plus” (hypermetropic) lens was used to magnify the perceived image of an enophthalmic and phthisical globe, to enhance appearance and improve symmetry. In the fourth patient, with Goldenhar syndrome, the appearance of a hypotropia and concurrent esotropia was successfully treated with a Fresnel prism and a prosthetic shell.
This case series illustrates the successful role of various refractive lenses often in conjunction with prosthetic shells in patients with reduced vision and orbital dystopia to improve facial symmetry. This conservative treatment is especially useful when surgery is not a desired or not considered a suitable option for the patient.
Long-term Pelvic Floor Symptoms, Recurrence, Satisfaction, and Regret Following Colpocleisis
Authors: Winkelman William D. , Haviland Miriam J. , Elkadry Eman A. 
Published: July 2018

Objectives
There are insufficient studies on long-term outcomes following colpocleisis, which limits physicians' ability to effectively counsel patients. The purpose of this study was to assess pelvic floor symptoms, recurrence, satisfaction, and regret among patients who underwent colpocleisis procedures.

Methods
This is an ambidirectional cohort study involving patients who underwent a colpocleisis at a single institution from 2002 to 2012. Medical records were reviewed, and patients were contacted by telephone in order to complete questionnaires.

Results
A total of 73 patients met inclusion criteria for our study. At the time of colpocleisis, patients were an average of 78.1 years old (range, 62–85 years). Patients were followed up in clinic for a median of 44.4 months, and the majority reported overactive bladder postoperatively. We were able to contact 33 patients by telephone. Median time between surgery and telephone follow-up was 6 years (range, 5–15 years). Among these patients, 78% were satisfied with the procedure; however, 13% reported strong feelings of regret. Regret was associated with postoperative bowel and bladder symptoms. The majority reported urinary frequency (63%) and urgency urinary incontinence (56%). Fewer than half (44%) of patients reported bowel symptoms. Only 19% reported prolapse symptoms. No patients reported regret due to loss of sexual function.

Conclusions
Colpocleisis remains an excellent surgical option for elderly patients. However, regret and dissatisfaction may increase over time as bowel and bladder symptoms may be perceived to be the result of surgery. Our findings highlight the continued need for detailed consent and expectation setting for women considering colpocleisis.
Exploration of Plant Biodiversity to Investigate Regulation of Photosynthesis in Natural Environment
Authors: Sello Simone , Alboresi Alessandro , Baldan Barbara , Morosinotto Tomas 
Published: July 2018

AbstractPhotosynthesis is regulated in response to dynamic environmental conditions to satisfy plant metabolic demand but also to avoid over-excitation of the electron transport chain generating harmful reactive oxygen species. Photosynthetic organisms evolved several mechanisms to modulate light harvesting and electron transport efficiency to respond to conditions changing at different timescales, going from fast sun flecks to slow seasonal variations.These regulatory mechanisms changed during evolution of photosynthetic organisms, also adapting to various ecological niches. The investigation of plant biodiversity is valuable to uncover conserved traits and plasticity of photosynthetic regulation. In this work a set of plants belonging to different genera of angiosperms, gymnosperms, ferns and lycophytes was investigated by monitoring their photosynthetic parameters in different seasons, looking for common trends and differences. In all plants analysed photosynthetic electron transport rate was found to be modulated by growth light intensity, ensuring a balance between available energy and photochemical capacity. Growth light also influenced the threshold where heat dissipation of excitation energy, also called Non-Photochemical Quenching (NPQ), was activated. On the contrary NPQ amplitude did not correlate with light intensity experienced by the plants but was a species-specific feature.NPQ zeaxanthin-dependent component, qZ, was found to be the most variable between different plants, modulating the intensity of the response but also the kinetic properties of its activation and relaxation. The slower NPQ component, qI, was instead found to be uncorrelated with photoinhibition eventually suffered by plants.