bluuebunny/crossref_metadata_2025_split huggingface dataset:
Arbuscular mycorrhizal fungi alleviate abiotic stresses in potato plants caused by low phosphorus and deficit irrigation/partial root-zone drying
Authors: Liu Caixia , Ravnskov Sabine , Liu Fulai , Rubæk Gitte H. , Andersen Mathias N. 
Published: February 2018

AbstractDeficit irrigation (DI) improves water use efficiency (WUE), but the reduced water input often limits plant growth and nutrient uptake. The current study examined whether arbuscular mycorrhizal fungi (AMF) could alleviate abiotic stress caused by low phosphorus (P) fertilization and DI.A greenhouse experiment was conducted with potato grown with (P1) or without (P0) P fertilization, with AMF (M1+:Rhizophagus irregularisor M2+:Glomus proliferum) or AMF-free control (M−) and subjected to full irrigation (FI), DI or partial root-zone drying (PRD).Inoculation of M1+ and M2+ maintained or improved plant growth and P/nitrogen (N) uptake when subjected to DI/PRD and P0. However, the positive responses to AMF varied with P level and irrigation regime. Functional differences were found in ability of AMF species alleviating plant stress. The largest positive plant biomass response to M1+ and M2+ was found under FI, both at P1 and P0 (25% increase), while plant biomass response to M1+ and M2+ under DI/PRD (14% increase) was significantly smaller. The large growth response to AMF inoculation, particularly under FI, may relate to greater photosynthetic capacity and leaf area, probably caused by stimulation of plant P/N uptake and carbon partitioning toward roots and tubers. However, plant growth response to AMF was not related to the percentage of AMF root colonization. Arbuscular mycorrhizal fungi can maintain and improve P/N uptake, WUE and growth of plants both at high/low P levels and under FI/DI. If this is also the case under field conditions, it should be implemented for sustainable potato production.
The Temporary Houses of Parliament and David Boswell Reid’s Architecture of Experimentation
Authors: Schoenefeldt Henrik 
Published: February 2018

what they may lose in dignity they will undoubtly [sic] gain in comfort and health.(The Champion, November 1836)The heating and ventilation of the two debating chambers in the British Houses of Parliament occupied the minds of various Select Committees and some of the country’s most renowned scientists and engineers during the nineteenth century. Indeed, since the seventeenth century the chambers had been major sites for technical experimentation in the field of ventilation, which, according to the Victorian physician Neil Arnott, reflected the technological and scientific advances made during this period. Nineteenth-century writers chronicled the design and testing of the numerous heating and ventilation systems that had been deployed. However, the strategies implemented during the eighteenth and early nineteenth centuries were not entirely successful in generating a pleasant internal environment. The destruction of the ancient Palace of Westminster by fire on 16 October 1834 therefore provided an important opportunity to design completely new debating chambers from first principles.
Web survey-based selection of controls for epidemiological analyses of a multi-prefectural outbreak of enterohaemorrhagicEscherichia coliO157 in Japan associated with consumption of self-grilled beef hanging tender
Authors: Yahata Y. , Ohshima N. , Odaira F. , Nakamura N. , Ichikawa H. , Ichikawa H. , Matsuno K. , Shuri J. , Toyozawa T. , Terajima J. , Watanabe H. , Nakashima K. , Sunagawa T. , Taniguchi K. , Okabe N. 
Published: February 2018

AbstractAn outbreak of enterohaemorrhagicEscherichia coliO157 occurred in multiple prefectures of Japan in November 2009. We conducted two case–control studies with trace-back and trace-forward investigations to determine the source. The case definition was met by 21 individuals; 14 (66.7%) were hospitalised, but no haemolytic uraemic syndrome, acute encephalopathy or deaths occurred. Median age was 23 (range 12–48) years and 14 cases were male (66.7%). No significant associations with food were found in a case–control study by local public health centres, but our matched case–control study using Internet surveys found that beef hanging tender (or hanger steak), derived from the diaphragm of the cattle, was significantly associated with illness (odds ratio = 15.77; 95% confidence interval, 2.00–124.11). Pulsed-field gel electrophoresis analysis of isolates from patients and the suspected food showed five different patterns: two in faecal and food samples, and another three in patient faecal samples only, although there were epidemiological links to the meat consumed at the restaurants. Trace-back investigation implicated a common food processing company from outside Japan. Examination of the logistics of the meat processing company suggested that contamination did not occur in Japan. We concluded that the source of the outbreak was imported hanging tender. This investigation revealed that Internet surveys could be useful for outbreak investigations.
Place, Performance and Identity in Monteverdi’sCombattimento di Tancredi e Clorinda
Authors: Cascelli Antonio 
Published: February 2018

AbstractWith its combination of gestures and music, instrumental sections, a narrator who occupies most of the composition, and two characters who sing for very short sections while acting and dancing for the rest of the piece, Monteverdi’sCombattimentodefies genre definition. Starting from Tim Carter’s reading of the composition as a salon entertainment and responding to Suzanne Cusick’s call for the untangling ofCombattimento’s multiplicity of meanings, this article investigatesCombattimentoin its ritualisation and performance of mutually defining relations that are mediated by the social and ideological implications of its immediate performance space, the salon – orportego, in Venetian dialect – the main entertainment hall of Venetian palaces. Using this as a key framework, the article explores theCombattimento’s associations with Venice itself as the broader performance space. Within that context, the choice of a particular episode from Tasso’sGerusalemme liberatafor Monteverdi’s composition – with its mixture of love and violence, assimilation and confrontation, personal identity and agency, history of winners and history of victims1– proves as crucial to seventeenth-century Venice, at the crossroads between Western and Islamic civilisations, as it does for today’s culture.
Opera and Monuments: Verdi’sErnaniin Vienna and the Construction of Dynastic Memory
Authors: Vellutini Claudio 
Published: February 2018

AbstractThis article examines the first production and early reception of Verdi’sErnaniin Vienna in relation to cultural processes supporting strategies of imperial representation promoted by the Habsburg Court. By discussing newspaper reviews, archival documents, sketches of the sets for the first Viennese production, I show how the opera responded to official narratives of the Habsburg Empire and to specific facets of the local culture of monumentality. I argue that particularly the Act III Finale functioned along the lines of memorial practices that projected the Habsburg dynasty as the source of unity of the empire and its peoples. Finally, I consider the Viennese reception ofErnaniin the broader context of its Italian dissemination, suggesting how fluctuating political readings of the opera depended upon (rather than undermining) multidirectional cultural exchanges.
Support needs of caregivers of patients with amyotrophic lateral sclerosis: A qualitative study
Authors: de Wit Jessica , Schröder Carin D. , el Mecky Julia , Beelen Anita , van den Berg Leonard H. , Visser-Meily Johanna M. A. 
Published: February 2018

AbstractObjectiveThe aim of this study was to explore the support needs of Dutch informal caregivers of patients with amyotrophic lateral sclerosis (ALS).MethodIndividual semi-structured interviews were conducted with 21 caregivers of ALS patients. Audio-taped interviews were transcribed and data were analyzed thematically.ResultA total of four global support needs emerged: “more personal time”, “assistance in applying for resources”, “counseling”, and “peer contact”. Despite their needs, caregivers are reluctant to apply for and accept support. They saw their own needs as secondary to the needs of the patients.Significance of resultsALS seems to lead to an intensive caregiving situation with multiple needs emerging in a short period. This study offers targets for the development of supportive interventions. A proactive approach seems essential, acknowledging the importance of the role of the caregivers in the care process at an early stage, informing them about the risk of burden, monitoring their wellbeing, and repeatedly offering support opportunities. Using e-health may help tailor interventions to the caregivers’ support needs.
Assessment of the reactions of pure lines selected from Turkish bread wheat landraces against bunt disease (Tilletia foetida) with the GGE-biplot method
Authors: Akçura Mevlüt , Akan Kadir 
Published: February 2018

AbstractThe present research was conducted to determine the reactions of 200 pure lines selected from bread wheat landraces collected from 18 provinces and seven regions of Turkey against bunt disease (Tilletia foetida) under field conditions for 3 years. Bunt disease reactions of pure lines were assessed based on the infected spike/total spike ratio. For visually assessed materials, the GGE-biplot method, where G = genotype effect and GE = genotype-by-environment effect, was used to group the reactions against bunt disease. Fifty-nine pure lines showed high resistance (with infection rates ranging from 0.1 to 10%); 24 in the moderate resistance (with infection rates ranging from 10.1 to 25%); 75 in the moderate susceptibility (with infection rates ranging from 25.1 to 45%); 38 in the susceptibility (with infection rates ranging from 45.1 to 70%) and finally four in the highly susceptibility (with infection rates of >70.1%). PC1 and PC2 of the GGE-biplot graph created over the years explained 76.49% of the total variation. The GGE-biplot graph provided efficient identification of resistant genotypes. The lowest PC1 values and PC2 values close to 0.0 explained the resistance of pure line to bunt disease best. The resistance of pure lines to bunt disease over the biplot decreased from the first section through the last section. Based on the results of present study, 19 pure lines (located within the first circle of the biplot graph) were selected for resistance breeding programmes against the diseases.
The costs of free: commoditization, bundling and concentration
Authors: BARNETT JONATHAN M. 
Published: February 2018

AbstractDigital markets offer abundant free content but exhibit extreme concentration among content aggregation intermediaries. These characteristics are linked. Weak copyright environments select against stand-alone content-delivery structures and select for bundled aggregation structures in which free content for users promotes positively priced advertising and data-collection services for firms. Dominant intermediaries promote commoditization, and the reallocation of market rents from content producers to content aggregators, through litigation and free content distribution that weaken copyright protections. The potential net welfare effects raise concern. Network effects, compounded by weak inventory constraints, scale economies, and learning effects, promote winner-takes-all outcomes in the intermediary services market while weak copyright may generate output distortions in the content production market.
TRIM36, a novel androgen-responsive gene, enhances anti-androgen efficacy against prostate cancer by inhibiting MAPK/ERK signaling pathways
Authors: Liang Chao , Wang Shangqian , Qin Chao , Bao Meilin , Cheng Gong , Liu Bianjiang , Shao Pengfei , Lv Qiang , Song Ninghong , Hua Lixin , Gu Min , Li Jie , Wang Zengjun 
Published: February 2018

AbstractHormone therapy drugs, such as bicalutamide and enzalutamide, directed against prostate cancer focus on androgen receptor (AR) signaling and are initially effective, but the disease progresses to lethality as resistance to these drugs develops. A method to prolong the drug response time and improve the drug efficacy is still unavailable. TRIM36 was reported as a novel androgen signaling target gene and is upregulated in prostate cancer. In this study, we found that 63.4% (64/95) of PCa in TMA expressed the TRIM36 protein. Interestingly, patients with negative TRIM36 expression had a shorter biochemical recurrence-free survival. TRIM36 expression was significantly associated with the Gleason score (P = 0.005), delayed prostate cancer cell cycle progression and inhibited cell proliferation in vitro and in vivo, and these effects were mediated via inhibition of the MAPK/ERK phosphorylation pathway. Remarkably, we found that rescuing the expression of TRIM36 during anti-androgen therapy could improve the drug efficacy. Collectively, TRIM36 is a novel androgen-responsive gene, and it dramatically enhanced the efficacy of anti-androgen drugs against prostate cancer.
Enantioselective [3+3] atroposelective annulation catalyzed by N-heterocyclic carbenes
Authors: Zhao Changgui , Guo Donghui , Munkerup Kristin , Huang Kuo-Wei , Li Fangyi , Wang Jian 
Published: February 2018

AbstractAxially chiral molecules are among the most valuable substrates in organic synthesis. They are typically used as chiral ligands or catalysts in asymmetric reactions. Recent progress for the construction of these chiral molecules is mainly focused on the transition-metal-catalyzed transformations. Here, we report the enantioselective NHC-catalyzed (NHC: N-heterocyclic carbenes) atroposelective annulation of cyclic 1,3-diones with ynals. In the presence of NHC precatalyst, base, Lewis acid and oxidant, a catalytic C–C bond formation occurs, providing axially chiral α-pyrone−aryls in moderate to good yields and with high enantioselectivities. Control experiments indicated that alkynyl acyl azoliums, acting as active intermediates, are employed to atroposelectively assemble chiral biaryls and such a methodology may be creatively applied to other useful NHC-catalyzed asymmetric transformations.
Similarity of stream width distributions across headwater systems
Authors: Allen George H. , Pavelsky Tamlin M. , Barefoot Eric A. , Lamb Michael P. , Butman David , Tashie Arik , Gleason Colin J. 
Published: February 2018

AbstractThe morphology and abundance of streams control the rates of hydraulic and biogeochemical exchange between streams, groundwater, and the atmosphere. In large river systems, the relationship between river width and abundance is fractal, such that narrow rivers are proportionally more common than wider rivers. However, in headwater systems, where many biogeochemical reactions are most rapid, the relationship between stream width and abundance is unknown. To constrain this uncertainty, we surveyed stream hydromorphology (wetted width and length) in several headwater stream networks across North America and New Zealand. Here, we find a strikingly consistent lognormal statistical distribution of stream width, including a characteristic most abundant stream width of 32 ± 7 cm independent of discharge or physiographic conditions. We propose a hydromorphic model that can be used to more accurately estimate the hydromorphology of streams, with significant impact on the understanding of the hydraulic, ecological, and biogeochemical functions of stream networks.
Combinatorial hydrogels with biochemical gradients for screening 3D cellular microenvironments
Authors: Vega Sebastián L. , Kwon Mi Y. , Song Kwang Hoon , Wang Chao , Mauck Robert L. , Han Lin , Burdick Jason A. 
Published: February 2018

Abstract3D microenvironmental parameters control cell behavior, but can be challenging to investigate over a wide range of conditions. Here, a combinatorial hydrogel platform is developed that uses light-mediated thiol-norbornene chemistry to encapsulate cells within hydrogels with biochemical gradients made by spatially varied light exposure. Specifically, mesenchymal stem cells are photoencapsulated in norbornene-modified hyaluronic acid hydrogels functionalized with gradients (0–5 mM) of peptides that mimic cell-cell or cell-matrix interactions, either as single or orthogonal gradients. Chondrogenesis varied spatially in these hydrogels based on the local biochemical formulation, as indicated by Sox9 and aggrecan expression levels. From 100 combinations investigated, discrete hydrogels are formulated and early gene expression and long-term cartilage-specific matrix production are assayed and found to be consistent with screening predictions. This platform is a scalable, high-throughput technique that enables the screening of the effects of multiple biochemical signals on 3D cell behavior.
Additive manufacturing of 3D nano-architected metals
Authors: Vyatskikh Andrey , Delalande Stéphane , Kudo Akira , Zhang Xuan , Portela Carlos M. , Greer Julia R. 
Published: February 2018

AbstractMost existing methods for additive manufacturing (AM) of metals are inherently limited to ~20–50 μm resolution, which makes them untenable for generating complex 3D-printed metallic structures with smaller features. We developed a lithography-based process to create complex 3D nano-architected metals with ~100 nm resolution. We first synthesize hybrid organic–inorganic materials that contain Ni clusters to produce a metal-rich photoresist, then use two-photon lithography to sculpt 3D polymer scaffolds, and pyrolyze them to volatilize the organics, which produces a >90 wt% Ni-containing architecture. We demonstrate nanolattices with octet geometries, 2 μm unit cells and 300–400-nm diameter beams made of 20-nm grained nanocrystalline, nanoporous Ni. Nanomechanical experiments reveal their specific strength to be 2.1–7.2 MPa g−1 cm3, which is comparable to lattice architectures fabricated using existing metal AM processes. This work demonstrates an efficient pathway to 3D-print micro-architected and nano-architected metals with sub-micron resolution.
Ultra-thin chips for high-performance flexible electronics
Authors: Gupta Shoubhik , Navaraj William Taube , Lorenzelli Leandro , Dahiya Ravinder 
Published: February 2018

AbstractFlexible electronics has significantly advanced over the last few years, as devices and circuits from nanoscale structures to printed thin films have started to appear. Simultaneously, the demand for high-performance electronics has also increased because flexible and compact integrated circuits are needed to obtain fully flexible electronic systems. It is challenging to obtain flexible and compact integrated circuits as the silicon based CMOS electronics, which is currently the industry standard for high-performance, is planar and the brittle nature of silicon makes bendability difficult. For this reason, the ultra-thin chips from silicon is gaining interest. This review provides an in-depth analysis of various approaches for obtaining ultra-thin chips from rigid silicon wafer. The comprehensive study presented here includes analysis of ultra-thin chips properties such as the electrical, thermal, optical and mechanical properties, stress modelling, and packaging techniques. The underpinning advances in areas such as sensing, computing, data storage, and energy have been discussed along with several emerging applications (e.g., wearable systems, m-Health, smart cities and Internet of Things etc.) they will enable. This paper is targeted to the readers working in the field of integrated circuits on thin and bendable silicon; but it can be of broad interest to everyone working in the field of flexible electronics.
Sleep endophenotypes of schizophrenia: slow waves and sleep spindles in unaffected first-degree relatives
Authors: D’Agostino Armando , Castelnovo Anna , Cavallotti Simone , Casetta Cecilia , Marcatili Matteo , Gambini Orsola , Canevini Mariapaola , Tononi Giulio , Riedner Brady , Ferrarelli Fabio , Sarasso Simone 
Published: February 2018

AbstractSleep spindles and slow waves are the main brain oscillations occurring in non-REM sleep. Several lines of evidence suggest that spindles are initiated within the thalamus, whereas slow waves are generated and modulated in the cortex. A decrease in sleep spindle activity has been described in Schizophrenia (SCZ), including chronic, early course, and early onset patients. In contrast, slow waves have been inconsistently found to be reduced in SCZ, possibly due to confounds like duration of illness and antipsychotic medication exposure. Nontheless, the implication of sleep spindles and slow waves in the neurobiology of SCZ and related disorders, including their heritability, remains largely unknown. Unaffected first-degree relatives (FDRs) share a similar genetic background and several neurophysiological and cognitive deficits with SCZ patients, and allow testing whether some of these measures are candidate endophenotypes. In this study, we performed sleep high-density EEG recordings to characterise the spatiotemporal features of sleep spindles and slow waves in FDRs of SCZ probands and healthy subjects (HS) with no family history of SCZ. We found a significant reduction of integrated spindle activity (ISAs) in FDRs relative to HS, whereas spindle density and spindle duration were not different between groups. FDRs also had decreased slow wave amplitude and slopes. Altogether, our results suggest that ISAs deficits might represent a candidate endophenotype for SCZ. Furthermore, given the slow wave deficits observed in FDRs, we propose that disrupted cortical synchronisation increases the risk for SCZ, but thalamic dysfunction is necessary for the disorder to fully develop.
A Mathematical Model for Enzyme Clustering in Glucose Metabolism
Authors: Jeon Miji , Kang Hye-Won , An Songon 
Published: February 2018

AbstractWe have recently demonstrated that the rate-limiting enzymes in human glucose metabolism organize into cytoplasmic clusters to form a multienzyme complex, the glucosome, in at least three different sizes. Quantitative high-content imaging data support a hypothesis that the glucosome clusters regulate the direction of glucose flux between energy metabolism and building block biosynthesis in a cluster size-dependent manner. However, direct measurement of their functional contributions to cellular metabolism at subcellular levels has remained challenging. In this work, we develop a mathematical model using a system of ordinary differential equations, in which the association of the rate-limiting enzymes into multienzyme complexes is included as an essential element. We then demonstrate that our mathematical model provides a quantitative principle to simulate glucose flux at both subcellular and population levels in human cancer cells. Lastly, we use the model to simulate 2-deoxyglucose-mediated alteration of glucose flux in a population level based on subcellular high-content imaging data. Collectively, we introduce a new mathematical model for human glucose metabolism, which promotes our understanding of functional roles of differently sized multienzyme complexes in both single-cell and population levels.