bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dormancy release and germination of Taxus yunnanensis seeds during wet sand storage
Authors: Bian Fangyuan , Su Jianrong , Liu Wande , Li Shuaifeng 
Published: February 2018

AbstractDormancy is an innate constraint on germination that occurs across all life forms. In this study, we investigated the seed dormancy release and germination characters of Taxus yunnanensis by exploring the seed morphology, permeability, germination inhibitors, endogenous hormones, and embryo germination in vitro during wet sand storage. Our results showed that seeds and embryos grew to a critical size to germination and permeability increased with the extension of storage. Seed coat and kernel methanol extracts reduced Brassica campestris seed vigor index. The in vitro embryo germination rate increased by 12.20% after storage for 30–360 d, whereas seed germination occurred after 450 d. Gibberellic acid and zeatin riboside contents were relatively stable, whereas abscisic acid (ABA) content decreased; indole acetic acid (IAA) content and the IAA/ABA ratio showed increasing trends. These results indicate that ABA is the key inhibitor of germination in Taxus. The chemical(s) in seed coat and kernel cause the inhibition of seed germination. Taken together, Taxus seeds have morphophysiological dormancy, in which the embryos can continue to grow and hormone imbalance inhibits further development and germination. Further, seed dormancy is active even during the middle of storage and shows “double peaks” during the entire dormancy process.
Plasmonic Spherical Heterodimers: Reversal of Optical Binding Force Based on the Forced Breaking of Symmetry
Authors: Mahdy M. R. C. , Danesh Md. , Zhang Tianhang , Ding Weiqiang , Rivy Hamim Mahmud , Chowdhury Ariful Bari , Mehmood M. Q. 
Published: February 2018

AbstractThe stimulating connection between the reversal of near-field plasmonic binding force and the role of symmetry-breaking has not been investigated comprehensively in the literature. In this work, the symmetry of spherical plasmonic heterodimer-setup is broken forcefully by shining the light from a specific side of the set-up instead of impinging it from the top. We demonstrate that for the forced symmetry-broken spherical heterodimer-configurations: reversal of lateral and longitudinal near-field binding force follow completely distinct mechanisms. Interestingly, the reversal of longitudinal binding force can be easily controlled either by changing the direction of light propagation or by varying their relative orientation. This simple process of controlling binding force may open a novel generic way of optical manipulation even with the heterodimers of other shapes. Though it is commonly believed that the reversal of near-field plasmonic binding force should naturally occur for the presence of bonding and anti-bonding modes or at least for the Fano resonance (and plasmonic forces mostly arise from the surface force), our study based on Lorentz-force dynamics suggests notably opposite proposals for the aforementioned cases. Observations in this article can be very useful for improved sensors, particle clustering and aggregation.
Ultrahigh resolution radiation imaging system using an optical fiber structure scintillator plate
Authors: Yamamoto Seiichi , Kamada Kei , Yoshikawa Akira 
Published: February 2018

AbstractHigh resolution imaging of radiation is required for such radioisotope distribution measurements as alpha particle detection in nuclear facilities or high energy physics experiments. For this purpose, we developed an ultrahigh resolution radiation imaging system using an optical fiber structure scintillator plate. We used a ~1-μm diameter fiber structured GdAlO3:Ce (GAP) /α-Al2O3 scintillator plate to reduce the light spread. The fiber structured scintillator plate was optically coupled to a tapered optical fiber plate to magnify the image and combined with a lens-based high sensitivity CCD camera. We observed the images of alpha particles with a spatial resolution of ~25 μm. For the beta particles, the images had various shapes, and the trajectories of the electrons were clearly observed in the images. For the gamma photons, the images also had various shapes, and the trajectories of the secondary electrons were observed in some of the images. These results show that combining an optical fiber structure scintillator plate with a tapered optical fiber plate and a high sensitivity CCD camera achieved ultrahigh resolution and is a promising method to observe the images of the interactions of radiation in a scintillator.
Operando characterization of cathodic reactions in a liquid-state lithium-oxygen micro-battery by scanning transmission electron microscopy
Authors: Liu Pan , Han Jiuhui , Guo Xianwei , Ito Yoshikazu , Yang Chuchu , Ning Shoucong , Fujita Takeshi , Hirata Akihiko , Chen Mingwei 
Published: February 2018

AbstractRechargeable non-aqueous lithium-oxygen batteries with a large theoretical capacity are emerging as a high-energy electrochemical device for sustainable energy strategy. Despite many efforts made to understand the fundamental Li-O2 electrochemistry, the kinetic process of cathodic reactions, associated with the formation and decomposition of a solid Li2O2 phase during charging and discharging, remains debate. Here we report direct visualization of the charge/discharge reactions on a gold cathode in a non-aqueous lithium-oxygen micro-battery using liquid-cell aberration-corrected scanning transmission electron microscopy (STEM) combining with synchronized electrochemical measurements. The real-time and real-space characterization by time-resolved STEM reveals the electrochemical correspondence of discharge/charge overpotentials to the nucleation, growth and decomposition of Li2O2 at a constant current density. The nano-scale operando observations would enrich our knowledge on the underlying reaction mechanisms of lithium-oxygen batteries during round-trip discharging and charging and shed lights on the strategies in improving the performances of lithium-oxygen batteries by tailoring the cathodic reactions.
Molecular characterization of colorectal adenomas with and without malignancy reveals distinguishing genome, transcriptome and methylome alterations
Authors: Druliner Brooke R. , Wang Panwen , Bae Taejeong , Baheti Saurabh , Slettedahl Seth , Mahoney Douglas , Vasmatzis Nikolaos , Xu Hang , Kim Minsoo , Bockol Matthew , O’Brien Daniel , Grill Diane , Warner Nathaniel , Munoz-Gomez Miguel , Kossick Kimberlee , Johnson Ruth , Mouchli Mohamad , Felmlee-Devine Donna , Washechek-Aletto Jill , Smyrk Thomas , Oberg Ann , Wang Junwen , Chia Nicholas , Abyzov Alexej , Ahlquist David , Boardman Lisa A. 
Published: February 2018

AbstractThe majority of colorectal cancer (CRC) arises from precursor lesions known as polyps. The molecular determinants that distinguish benign from malignant polyps remain unclear. To molecularly characterize polyps, we utilized Cancer Adjacent Polyp (CAP) and Cancer Free Polyp (CFP) patients. CAPs had tissues from the residual polyp of origin and contiguous cancer; CFPs had polyp tissues matched to CAPs based on polyp size, histology and dysplasia. To determine whether molecular features distinguish CAPs and CFPs, we conducted Whole Genome Sequencing, RNA-seq, and RRBS on over 90 tissues from 31 patients. CAPs had significantly more mutations, altered expression and hypermethylation compared to CFPs. APC was significantly mutated in both polyp groups, but mutations in TP53, FBXW7, PIK3CA, KIAA1804 and SMAD2 were exclusive to CAPs. We found significant expression changes between CAPs and CFPs in GREM1, IGF2, CTGF, and PLAU, and both expression and methylation alterations in FES and HES1. Integrative analyses revealed 124 genes with alterations in at least two platforms, and ERBB3 and E2F8 showed aberrations specific to CAPs across all platforms. These findings provide a resource of molecular distinctions between polyps with and without cancer, which have the potential to enhance the diagnosis, risk assessment and management of polyps.
Enhancement of immunomodulative effect of lactic acid bacteria on plasmacytoid dendritic cells with sucrose palmitate
Authors: Kanayama Masaya , Kato Yukiko , Tsuji Toshikazu , Konoeda Yuki , Hashimoto Akiko , Kanauchi Osamu , Fujii Toshio , Fujiwara Daisuke 
Published: February 2018

AbstractPlasmacytoid dendritic cells (pDCs) play a key role in the immune response against viruses. In addition, recent research has suggested that pDCs possess direct and indirect tumoricidal activities. We previously found that a lactic acid bacteria strain, Lactococcus lactis JCM 5805 (LC-Plasma), stimulated pDCs and prevented viral infection in mouse and human studies. Meanwhile, emulsifiers have recently been highlighted as candidate adjuvants for some viral vaccines and cancer immunotherapies. In this study, we discovered some specific emulsifiers, mainly consisting of sucrose fatty acid esters, that drastically enhance the potency of LC-Plasma to activate pDCs in vitro. The emulsifiers promoted the efficient uptake of LC-Plasma by pDCs and the ratio of pDCs that took up LC-Plasma correlated with the activity of pDCs. In addition, an in vivo study showed that oral treatment with LC-Plasma mixed with an emulsifier induced a higher expression of genes related to anti-viral immunity in the lung compared to treatment with LC-Plasma alone. Both LC-Plasma and the emulsifiers used in this study have been confirmed to be safe for human use. Therefore, LC-Plasma mixed with an emulsifier might be a useful tool for certain anti-cancer and anti-viral therapies.
Georeferenced soil provenancing with digital signatures
Authors: Tighe M. , Forster N. , Guppy C. , Savage D. , Grave P. , Young I. M. 
Published: February 2018

AbstractThe provenance or origin of a soil sample is of interest in soil forensics, archaeology, and biosecurity. In all of these fields, highly specialized and often expensive analysis is usually combined with expert interpretation to estimate sample origin. In this proof of concept study we apply rapid and non-destructive spectral analysis to the question of direct soil provenancing. This approach is based on one of the underlying tenets of soil science – that soil pedogenesis is spatially unique, and thus digital spectral signatures of soil can be related directly, rather than via individual soil properties, to a georeferenced location. We examine three different multivariate regression techniques to predict GPS coordinates in two nested datasets. With a minimum of data processing, we show that in most instances Eastings and Northings can be predicted to within 20% of the range of each within the dataset using the spectral signatures produced via portable x-ray fluorescence. We also generate 50 and 95% confidence intervals of prediction and express these as a range of GPS coordinates. This approach has promise for future application in soil and environmental provenancing.
Chronic treatment with fluoride affects the jejunum: insights from proteomics and enteric innervation analysis
Authors: Dionizio Aline Salgado , Melo Carina Guimarães Souza , Sabino-Arias Isabela Tomazini , Ventura Talita Mendes Silva , Leite Aline Lima , Souza Sara Raquel Garcia , Santos Erika Xavier , Heubel Alessandro Domingues , Souza Juliana Gadelha , Perles Juliana Vanessa Colombo Martins , Zanoni Jacqueline Nelisis , Buzalaf Marília Afonso Rabelo 
Published: February 2018

AbstractGastrointestinal symptoms are the first signs of fluoride (F) toxicity. In the present study, the jejunum of rats chronically exposed to F was evaluated by proteomics, as well as by morphological analysis. Wistar rats received water containing 0, 10 or 50 mgF/L during 30 days. HuC/D, neuronal Nitric Oxide (nNOS), Vasoactive Intestinal Peptide (VIP), Calcitonin Gene Related Peptide (CGRP), and Substance P (SP) were detected in the myenteric plexus of the jejunum by immunofluorescence. The density of nNOS-IR neurons was significantly decreased (compared to both control and 10 mgF/L groups), while the VIP-IR varicosities were significantly increased (compared to control) in the group treated with the highest F concentration. Significant morphological changes were seen observed in the density of HUC/D-IR neurons and in the area of SP-IR varicosities for F-treated groups compared to control. Changes in the abundance of various proteins correlated with relevant biological processes, such as protein synthesis, glucose homeostasis and energy metabolism were revealed by proteomics.
Revisiting the Pacific Meridional Mode
Authors: Stuecker Malte F. 
Published: February 2018

AbstractNumerous studies demonstrated that the Pacific Meridional Mode (PMM) can excite Central Pacific (CP) El Niño-Southern Oscillation (ENSO) events and that the PMM is mostly a stochastic phenomenon associated with mid-latitude atmospheric variability and wind-evaporation-SST feedback. Here we show that CP sea surface temperature (SST) variability exhibits high instantaneous correlations both on interannual (ENSO-related) and decadal (Pacific Decadal Oscillation (PDO)-related) timescales with the PMM. By prescribing an idealized interannual equatorial CP ENSO SST forcing in a partially-coupled atmosphere/slab ocean model we are able to generate a realistic instantaneous PMM response consistent with the observed statistical ENSO/PMM relationship. This means that CP ENSO and the PMM can excite each other respectively on interannual timescales, strongly suggesting that a fast positive feedback exists between the two phenomena. Thus, we argue that they cannot be considered two independent dynamical entities. Additionally, we show that the interannual CP ENSO SST forcing generates atmospheric circulation variability that projects strongly on the Aleutian Low and North Pacific SST anomalies that exhibit the characteristic PDO pattern.
Elevated nitrogen allows the weak invasive plant Galinsoga quadriradiata to become more vigorous with respect to inter-specific competition
Authors: Liu Gang , Yang Ying-Bo , Zhu Zhi-Hong 
Published: February 2018

AbstractElevated nitrogen associated with global change is believed to promote the invasion of many vigorous exotic plants. However, it is unclear how a weak exotic plant will respond to elevated nitrogen in the future. In this study, the competitive outcome of a weak invasive plant (Galinsoga quadriradiata) and two non-invasive plants was detected. The plants were subjected to 3 types of culture (mixed, monoculture or one-plant), 2 levels of nitrogen (ambient or elevated at a rate of 2 g m−2 yr−1) and 2 levels of light (65% shade or full sunlight). The results showed that elevated nitrogen significantly promoted the growth of both the weak invader and the non-invasive plants in one-plant pots; however, growth promotion was not observed for the non-invasive species in the mixed culture pots. The presence of G. quadriradiata significantly inhibited the growth of the non-invasive plants, and a decreased negative species interaction was detected as a result of elevated nitrogen. Our results suggest that competitive interactions between G. quadriradiata and the non-invasive plants were altered by elevated nitrogen. It provides exceptional evidence that an initially weak invasive plant can become an aggressive invader through elevated nitrogen deposition.
Phenotypic plasticity vs. local adaptation in quantitative traits differences of Stipa grandis in semi-arid steppe, China
Authors: Gao Shao-bo , Mo Li-dong , Zhang Li-hong , Zhang Jian-li , Wu Jian-bo , Wang Jin-long , Zhao Nian-xi , Gao Yu-bao 
Published: February 2018

AbstractWhether plants are able to adapt to environmental changes depends on their genetic characteristics and phenotypic plastic responses. We investigated the phenotypic responses of 7 populations of an important dominant species in semi-arid steppe of China - Stipa grandis, and then distinguished which adaptive mechanism(s), phenotypic plasticity or local adaptation, was/were involved in this species to adapt to environmental changes. (1) All traits were significantly influenced by the interaction of population and growth condition and by population in each condition, and inter-population variability (CVinter) was larger in the field than in the common garden for 8/9 traits, indicating that both phenotypic plasticity and genetic differentiation controlled the phenotypic differences of S. grandis. (2) From a functional standpoint, the significant relationships between the values of traits in the common garden and the environmental variables in their original habitats couldn’t support local habitat adaptation of these traits. (3) Low CVintra, low quantitative differentiation among populations (Q
ST
), and low plasticity shown in the western populations indicated the very low adaptive potential of S. grandis to environmental changes. (4) From the original habitats to the common garden which is far away from S. grandis distribution region, positive phenotypic responses were found in several populations, indicating that some original habitats have become unfavorable for S. grandis.
A Potential Prognostic Long Noncoding RNA Signature to Predict Recurrence among ER-positive Breast Cancer Patients Treated with Tamoxifen
Authors: Wang Kang , Li Jie , Xiong Yong-Fu , Zeng Zhen , Zhang Xiang , Li Hong-Yuan 
Published: February 2018

AbstractLimited predictable long noncoding RNA (lncRNA) signature was reported in tamoxifen resistance among estrogen receptor (ER)-positive breast cancer (BC) patients. The aim of this study was to identify and assess prognostic lncRNA signature to predict recurrence among ER-positive BC patients treated with tamoxifen. Cohorts from Gene Expression Omnibus (GEO) (n = 298) and The Cancer Genome Atlas (TCGA) (n = 160) were defined as training and validation cohort, respectively. BC relapse associated lnRNAs was identify within training cohort, and the predictable value of recurrence was assessed in both cohorts. A total of 11lncRNAs were recognized to be associated with relapse free survival (RFS) of ER-positive BC patients receiving tamoxifen, who were divided into low-risk and high-risk group on basis of relapse risk scores (RRS). Multivariate cox regression analyses revealed that the RRS is an independent prognostic biomarker in the prediction of ER-positive BC patients’ survival. GSEA indicated that high-risk group was associated with several signaling pathways in processing of BC recurrence and metastasis such as PI3K-Akt and Wnt signaling. Our 11-lncRNA based classifier is a reliable prognostic and predictive tool for disease relapse in BC patients receiving tamoxifen.
Effect of chronic exercise on fluoride metabolism in fluorosis-susceptible mice exposed to high fluoride
Authors: Amaral Sandra L. , Azevedo Liane B. , Buzalaf Marilia A. R. , Fabricio Mayara F. , Fernandes Mileni S. , Valentine Ruth A. , Maguire Anne , Zohoori Fatemeh V. 
Published: February 2018

AbstractThe present study investigated the effect of chronic exercise on fluoride (F) metabolism in fluorosis-susceptible mice exposed to high-F and explored the relationship between F concentrations in bone and plasma. Thirty male mice were randomised into three groups: Group I (No-F, No-Exercise), Group II (50 ppmF, No-Exercise), Group III (50 ppmF, Exercise). Body weight and physical performance of all mice were measured at baseline and end of experiment. F concentrations of plasma and bone were measured at the end of experiment. Mean plasma F concentration was significantly higher (p < 0.001) in Groups II and III compared with Group I. Mean bone F concentration was also significantly higher (p < 0.01) in Groups II and III compared with Group I. There was a significant correlation (p = 0.01, r = 0.54) between F concentration of plasma and bone. Mean body weight of Group I mice was significantly higher than Group II (p < 0.001) and Group III (p = 0.001) mice at the end of the experiment. This study, which provides the first data on the effect of chronic exercise on F metabolism in fluorosis-susceptible mice, suggests no effect of chronic exercise on F in plasma and bone. However, exposure to high-F resulted in lower body weight and exercise capacity in mice.
Effect of Cord Blood Magnesium Level at Birth on Non-neurologic Neonatal Outcomes
Authors: Edwards Laura , Swamy Geeta , Grotegut Chad , Edwards James 
Published: February 2018

Objective We examined the effects of magnesium sulfate on non-neurologic neonatal outcomes with respect to cord blood magnesium level.
Study Design We conducted a secondary analysis of the Maternal-Fetal Medicine Units Beneficial Effects of Antenatal Magnesium (MFMU BEAM) trial comparing the upper and lower quintiles of cord blood magnesium level. Outcomes included cerebral palsy (CP), necrotizing enterocolitis (NEC), retinopathy of prematurity (ROP), bronchopulmonary dysplasia (BPD), and assessments of mental and motor disability. Logistic regression was used to estimate adjusted odds ratios (aORs) of each outcome, controlling for gestational age (GA), birth weight, and treatment group (TG).
Results A total of 1,254 women of the 2,444 included in the BEAM trial had cord blood magnesium levels recorded. GA and birth weight were lower and TG was more common in the upper quintile cohort (p < 0.001). Neonates in the upper quintile were more likely to have severe NEC (OR, 2.41, 95% confidence interval [CI]: 1.11–5.24), ROP (OR, 1.65, 95% CI: 1.05–2.59), and BPD (OR, 1.70, 95% CI: 1.04–2.73). Adjustment for covariates demonstrated no difference in the NEC, ROP, and BPD rates, although there was a decrease in rates of mental disability index < 70 which was not seen in the unadjusted analysis (aOR, 0.49, 95% CI: 0.25–0.99).
Conclusion Higher cord blood magnesium levels do not appear to have adverse non-neurologic effects on the neonate and may demonstrate improvement in neurologic outcomes.