bluuebunny/crossref_metadata_2025_split huggingface dataset:
A multi-institutional trial evaluating the use of an integrated quality assurance phantom for frameless single-isocenter multitarget stereotactic radiosurgery
Authors: Capaldi Dante P. I. , Skinner Lawrie B. , Pinkham Daniel W. , Zavgorodni Sergei , Stafford Olga , Shirmohammad Maryam , Matney Jason E. , Dubrowski Piotr , De Jean Paul , Grafil Elliot M. , Yu Amy S. 
Published: October 2024

BackgroundBrain radiosurgery treatments require multiple quality-assurance (QA) procedures to ensure accurate and precise treatment delivery of ablative doses. As single-isocenter multitarget radiosurgery treatments become more popular for treating patients with multiple brain metastases, quantifying off-axis accuracy of linear accelerators is crucial. In this study, we developed a novel brain radiosurgery integrated phantom and validated this phantom at multiple institutions to enable radiosurgery QA with a single phantom to facilitate implementation of a frameless single-isocenter, multitarget radiosurgery program. The phantom combines multiple independent verification system tests including the Winston-Lutz test, off-axis accuracy evaluation (i.e., off-axis Winston-Lutz), as well as dosimetric measurements utilizing both point dose and film measurement.Methods and materialsA novel 3D-printed phantom, coined OneIso, was designed with a movable insert which can switch between Winston-Lutz test targets and dose measurement without moving the phantom itself. In total, four phantoms were printed, and eight institutions participated in this study, which included both Varian TrueBeam (n=6) and Elekta Versa (n=2) linear accelerators. For off-axis Winston-Lutz measurements, a row of off-axis ball-bearings (BBs) was integrated into the OneIso. To quantify the spatial accuracy versus distance from isocenter, two-dimensional displacements were calculated between the planned and delivered BB locations relative to their respective MLC-defined field borders. For dose verification, brain radiosurgery clinical treatment plans previously treated were delivered at multiple cancer centers (six of eight centers). Radiochromic film and pinpoint ion chamber comparison measurements were obtained with OneIso.ResultsDose verification performed using the OneIso phantom across the different centers were all within on average 3% agreement, for both film and point-dose measurements. OneIso identified a reduction in spatial accuracy further away from isocenter for all eight radiosurgery machines. Differences increased as distance from isocenter increased, exceeding recommended radiosurgery accuracy tolerances (&lt;1mm) at different distances for each machine (2-7cm), indicating that the tolerance is machine-dependent.ConclusionOneIso provides a streamlined, single-setup workflow for single-isocenter multitarget frameless linac-based radiosurgery QA that can be easily translated to multiple institutions. Additionally, quantifying off-axis spatial discrepancies allows for determination of the maximum distance between targets and iso that meet single-isocenter multitarget radiosurgery program recommendations.
Safety and efficacy of combined acetabular reconstruction and microwave ablation in the treatment of periacetabular metastatic disease: a retrospective clinical evaluation
Authors: Zheng Chuanxi , Qiu Jin , Zhou Xiayi , Xu Gang , Lan Tao , Zhang Shiquan , Li Wei 
Published: October 2024

IntroductionThe periacetabular bone defects caused by metastatic disease often necessitate acetabular reconstruction and various techniques have been employed with varying degrees of success. The purpose of this study was to evaluate the efficacy and safety of acetabular reconstruction in conjunction with adjuvant microwave ablation as a surgical intervention for patients with periacetabular metastases.MethodsBetween January 2019 and September 2023, 17 consecutive patients with different tumor subtypes required surgical intervention for periacetabular metastases. The acetabular reconstruction was performed by utilizing an acetabular reconstructive cage and cement total hip arthroplasty with microwave ablation. A retrospective review was performed to assess pain relief, intraoperative details and postoperative complications. Functional status following procedures was determined by the 1993 Musculoskeletal Tumor Society (MSTS) score and the overall survival of patients was estimated by Kaplan-Meier analysisResultsIn total, 8 males and 9 females were included with an average age of 48.6 years (range 34-66). Patients reported a significant improvement in the level of pain and the mean VAS score declined from 7.7 preoperatively to 2.2 postoperatively. Of the 17 patients, 16 could ambulate either independently (6 patients) or using a walking aid (10 patients) with a mean MSTS score of 18.9. The median follow‐up was 9.0 months. Nine patients were alive at the most recent follow-up with overall survival of 40.9% at 12 months and 30.7% at 36 months, respectively.ConclusionIn selected patients with periacetabular metastasis, the utilization of an acetabular cage and cement total hip arthroplasty presents a less invasive reconstruction technique. The incorporation of adjuvant microwave ablation has shown promise in providing long-lasting pain relief, reducing intraoperative bleeding, and improving local tumor control. However, further research and extended follow-up are necessary to establish the effectiveness of this procedure.
Biomaterial-mediated delivery of traditional Chinese medicine ingredients for spinal cord injury: a systematic review
Authors: Liu Gang , Pei Zhenzhen , Bai Huizhong , Huo Luyao , Deng Bowen , Jiang Shengyuan , Tao Jingwei , Xu Lin , Li Jinyu , Gao Feng , Mu Xiaohong 
Published: October 2024

ObjectiveBiomaterials loaded with ingredients derived from traditional Chinese medicine (TCM) are viewed as a promising strategy for treating spinal cord injury (SCI). However, a comprehensive analysis of the existing literature on this topic has not yet been conducted. Therefore, this paper systematically reviews researches related to this approach, aiming to identify gaps and shortcomings in the field.MethodsPubMed, EMBASE, Web of Science, Chinese Biomedical Literature, Wanfang, and China National Knowledge Infrastructure (CNKI) were searched for retrieving studies on biomaterials loaded with TCM ingredients published from their inception to October 2024. Two reviewers performed screening of search results, information extraction, and literature quality assessment independently.ResultsFor this systematic review, 41 publications were included. Six TCM ingredients-paclitaxel, curcumin, tetramethylpyrazine, resveratrol, berberine, and tanshinone IIA were combined with biomaterials for treatment of SCI. Biomaterials were categorized into hydrogels, biodegradable scaffolds, nanoparticles, and microspheres according to the type of scaffold. These drug delivery systems exhibit commendable biocompatibility, drug-loading capacity, and drug-release capabilities, and in combination with TCM ingredients, synergistically contribute to anti-oxidative stress, anti-inflammatory, neuroprotective, and anti-apoptotic effects.ConclusionThese studies demonstrated the efficacy of biomaterials loaded with TCM ingredients in facilitating motor function recovery and neuroprotection in SCI rats, providing evidence for future research. However, in the complex microenvironment of SCI, achieving the maximum drug loading capacity of TCM ingredients within biomaterials, along with sustained and controlled release to fully exert their pharmacological effects, remains a major challenge for future research.Systematic Review Registrationhttps://www.crd.york.ac.uk/PROSPERO/ identifier CRD42024505000.
Codonopsis pilosula seedling drought- responsive key genes and pathways revealed by comparative transcriptome
Authors: Wang Hongyan , Chen Yuan , Liu Lanlan , Guo Fengxia , Liang Wei , Dong Linlin , Dong Pengbin , Cheng Jiali , Chen Yongzhong 
Published: October 2024

BackgroundCodonopsis pilosula (Campanulaceae) is a traditional herbal plant that is widely used in China, and the drought stress during the seedling stage directly affects the quality, ultimately impacting its yield. However, the molecular mechanisms underlying the drought resistance of C. pilosula seedlings remain unclear.MethodHerein, we conducted extensive comparative transcriptome and physiological studies on two distinct C. pilosula cultivar (G1 and W1) seedlings subjected to a 4-day drought treatment.ResultsOur findings revealed that cultivar G1 exhibited enhanced retention of proline and chlorophyll, alongside a marked elevation in peroxidase activity, coupled with diminished levels of malondialdehyde and reduced leaf relative electrolyte leakage compared with cultivar W1. This suggested that cultivar G1 had relatively higher protective enzyme activity and ROS quenching capacity. We discerned a total of 21,535 expressed genes and identified 4,192 differentially expressed genes (DEGs) by RNA sequencing (RNA-seq). Our analysis revealed that 1,764 DEGs unique to G1 underwent thorough annotation and functional categorization utilizing diverse databases. Under drought conditions, the DEGs in G1 were predominantly linked to starch and sucrose metabolic pathways, plant hormone signaling, and glutathione metabolism. Notably, the drought-responsive genes in G1 were heavily implicated in hormonal modulation, such as ABA receptor3-like gene (PYL9), regulation by transcription factors (KAN4, BHLH80, ERF1B), and orchestration of drought-responsive gene expression. These results suggest that cultivar G1 possesses stronger stress tolerance and can better adapt to drought growing conditions. The congruence between qRT-PCR validation and RNA-seq data for 15 DEGs further substantiated our findings.ConclusionOur research provides novel insights into the physiological adaptations of C. pilosula to arid conditions and lays the groundwork for the development of new, drought-tolerant C. pilosula cultivars.
Effects of mindfulness intervention on competition state anxiety in sprinters—a randomized controlled trial
Authors: Yu Ming Ge , Dou Guang Bo , Gong Chen 
Published: October 2024

ObjectivesWith the rapid growth of China’s sprint program’s international competitiveness, the psychological problems of sprinters have become a common concern in sports training theory and practice. Hence, the study examined the impact of a 7-week Mindfulness training program on competition state anxiety in Chinese sprinters.MethodsTwenty-four sprinters (Mage = 22.46 ± 1.351) were selected in a 2 × 3 mixed design, with the group (mindfulness/control) as the between-subjects variable and test time (pre-test/mid-test/post-test) as the within-subjects variable. The dependent variables corresponded to the mindfulness score and competition state anxiety score. One 60-min session was conducted once a week for 7 weeks, and the control group did not undergo any psychological training. The mindfulness group received mindfulness training, and the control group received regular psychological guidance. The subjects filled in the Five Facet Mindfulness Questionnaire (FFMQ) and the Competition State Anxiety Scale at baseline, followed by additional assessments 3 weeks and 7 weeks later.Results(1) In terms of competition state anxiety, there were no significant differences in the pre-test between the mindfulness group and the control group. There were significant differences in sprinters’ competition state anxiety after mindfulness intervention in terms of time, group, and the interaction between time and groups (p = 0.03, 0.004, and 0.009). (2) In terms of the mindfulness level, the difference between the mindfulness group and the control group was not significant in the pre-test. The sprinters’ mindfulness level was significant in the interaction between groups and that between groups and time after mindfulness intervention (p = 0.027 and 0.028).ConclusionMindfulness training alleviated sprinters’ competition anxiety by reducing sprinters’ somatic state anxiety (SSA) and cognitive state anxiety (CSA), as well as improving state self-efficacy. The results provide guidance and references for Chinese sprinters’ psychological problems.
Mendelian randomization analysis of causal and druggable circulating inflammatory proteins in schizophrenia
Authors: Cao Hongbao , Fu Li , Liu Dongming , Baranova Ancha , Zhang Fuquan 
Published: October 2024

BackgroundSchizophrenia (SZ) is a severe mental disorder with complex origins. Observational studies suggested that inflammatory factors may play a role in the pathophysiology of SZ and we aim to investigate the potential genetic connection between them by examining the causal impact of circulating inflammatory proteins on SZ.MethodsWe utilized Mendelian randomization (MR) analysis to assess the causal relationship between circulating inflammatory proteins and SZ and the GWAS summary datasets were sourced from public databases. The SZ dataset comprised 74,776 cases and 101,023 controls, while the summary results for 91 plasma proteins in 14,824 participants were obtained through the Olink Target platform. Moreover, to identify and evaluate potential drug targets, we searched the Drug-Gene Interaction Database (DGIdb).ResultsThe results of the MR study confirmed that nine inflammatory proteins had a causal effect on SZ. Among these proteins, IL1A (OR: 0.93), TNFB (OR: 0.94), TNFSF14 (OR: 0.96), and CD40 (OR: 0.95) exhibited protective effects against SZ. Conversely, CCL23 (OR: 1.04), CCL19 (OR: 1.04), 4EBP1 (OR: 1.06), TWEAK (OR: 1.08), and DNER (OR: 1.10) were associated with an increased risk of SZ. The MR-Egger and weighted median methods also supported the direction of these effects. According to the Gene-Drug analysis, LTA, IL1A, CD40, and 4EBP1 can serve as drug targets.ConclusionsOur study established causal relationships between circulating inflammatory proteins and SZ. It may be beneficial to personalize the treatment of SZ by incorporating inflammation management into the treatment regimen.
Insights from older adults’ lived experience of physical activity and exercise during the COVID-19 lockdown in England
Authors: Szekeres Zsofia , Agustín-Sierra Noelia , Zaidell Lisa , Mileva Katya N. , De Oliveira Rita F. 
Published: October 2024

IntroductionThis study investigated older adults' emotional and social experiences of physical activity and exercise during the first Covid-19 lockdown in England.MethodsParticipants were 24 older adults (M = 74 years, SD = 5.0) either physically active or inactive before lockdown. Semi-structured interviews were conducted at the start of the pandemic in England, and when restrictions were lifted eight weeks later.ResultsTemplate analysis revealed three main themes: a Sense of Purpose for Being Physically Active, Routes for Engagement, and Inactive by Force? with different sub-themes for active and inactive participants. The lockdown restrictions emphasised the need to keep physically active in both active and inactive participants, but they perceived barriers differently. Both active and inactive participants found a renewed sense of purpose in walking outdoors for exercise when restrictions eased, enhancing their physical and mental well-being.DiscussionTo reduce barriers and emphasise that exercise is for all ages and all levels of mobility, multi-modal recommendations are presented for educating, promoting, supporting, and enabling older adults to engage in physical activity and exercise.
Understanding the Risk Factors, Burden, and Interventions for Chronic Respiratory Diseases in Low- and Middle-Income Countries: A Scoping Review
Authors: Boutros Perla , Kassem Nour , Boudo Valentin , Sié Ali , Munga Stephen , Maggioni Martina A. , Golec Marcin , Simion Robin , Bärnighausen Till , Winkler Volker , Barteit Sandra 
Published: October 2024

ObjectiveThis scoping review aims to identify risk factors for COPD and asthma, examine the burden and intervention measures, and clarify the findings in the context of climate change, with a particular focus on LMICs.MethodsFollowing the PRISMA-ScR guidelines, we conducted a scoping review using PubMed, Embase, and Scopus, focusing on studies published from 2011 to 2024.ResultsOur review included 52 studies that encompassed 244,004 participants. Predominantly conducted in SSA (n = 43, 83%) and Asia (n = 16, 31%), they address indoor and ambient air pollution, occupational hazards, and environmental conditions. Climate change exacerbates risks, varying regionally. SSA faces severe household and occupational exposures, while other LMICs deal with industrial and urban pollution. Stigma, social exclusion and economic burden underscore the necessity for intervention strategies (e.g., educational programs, pulmonary rehabilitation, low-emission cookstoves).ConclusionOur research shows a strong link between air pollution, occupational and environmental exposures, and the prevalence of COPD and asthma in LMICs. It suggests that targeted interventions are effective ways to mitigate these diseases and also highlights the significant impact of climate change on respiratory health.
Developing Adventitious Root Meristems Induced by Layering for Plant Chromosome Preparation
Authors: Yan Xu , Wu Zizhou , Wang Honglin , Zuo Yanchun , Du Zhouhe 
Published: October 2024

Chromosome numbers and morphology are important characteristics of a species and its evolution. Root tips are the most commonly used tissue as a source of actively dividing cells for chromosome visualization in plants. Previously, rapidly growing root tips were collected from germinating kernels or from seedlings growing in pots or fields. However, the use of adventitious roots (ARs) derived from aerial tissue as meristems for chromosome visualization has always been overlooked. Here, we successfully induced ARs in 12 materials that were investigated, with the exception of Sorghum nitidum. Using ARs meristem we obtained high-quality chromosome spreads for Morus alba, Broussonetia papyrifera, Lolium multiflorum, Sorghum sudanense, S. propinquum, S. bicolor × S. sudanense, Zea mays, Z. mexicana, Glycine max, Medicago sativa, and Brassica napus. The results reported here demonstrate that layering is an alternative and effective method for producing meristematic cells for high-quality chromosome preparation in plant species producing ARs. For species that produce ARs by layering, this protocol is particularly valuable for the development of cost-effective and high-throughput non-invasive cytogenetic studies.
Methanolic Extract of the Nutritional Plant (Diospyros kaki Thunb.) Exhibits Anticancer Activity by Inducing Mitochondrial Dysfunction in Colorectal Cancer Cells
Authors: Bianchini Stefano , Bovio Federica , Negri Stefano , Bisson Leonardo , Piccinelli Anna Lisa , Rastrelli Luca , Forcella Matilde , Fusi Paola 
Published: October 2024

Background/Objectives: Diospyros kaki, the most widely cultivated species of persimmon, has been long used in traditional medicine since its leaves’ extracts contain high amounts of flavonoids and terpenoids, endowed with several beneficial effects. However, its anticancer activity towards colorectal cancer (CRC) has not been investigated in depth. Methods: The effect of a methanolic extract of D. kaki leaves, rich in kaempferol and quercetin derivatives, have been evaluated on an E705 CRC cell line, representative of most CRC patients, and on SW480 cells, carrying a KRAS-activating mutation. Results: This extract is effective in reducing tumor cells’ viability without affecting the healthy mucosa cell line CCD 841. In fact, Western blot experiments showed its ability to induce apoptosis in cancer cells by increasing oxidative stress and disrupting mitochondrial functionality, as shown by reactive oxygen species measurement and Seahorse analysis. Conclusions: With the aim of increasing healthspan, as well as the substantial societal and macroeconomic costs associated with cancer, our results could pave the way to a role for D. kaki extract in both CRC treatment and prevention.
Sistem Pendukung Keputusan Dalam Menentukan Siswa Berprestasi Menggunakan Metode Simple Additive Weighting (Studi Kasus: MA Sahid)
Authors: Nurul Kamilah  , Adhitya Nurachman Muhammad , Dewi Primasari  
Published: October 2024

Madrasah Aliyah Sahid selalu mengadakan pemilihan siswa berprestasi setiap semester. Pemilihan siswa berprestasi tersebut dilakukan perkelas dengan menentukan nilai yang tertinggi dari peringkat 1 sampai 3. Namun penilaian siswa berprestasi masih dilakukan hanya berdasarkan pada nilai raport saja, sehingga aspek lain selain nilai raport tidak dijadikan bahan pertimbangan sebagai kriteria siswa berprestasi. Hal ini menimbulkan berbagai permasalahan seperti siswa yang memiliki nilai raport bagus tetapi memiliki kekurangan di beberapa aspek lain, yang berarti siswa berprestasi yang dipilih tidak mencapai standar yang dibutuhkan dan tidak memperoleh kandidat yang terbaik. Dalam menangani masalah tersebut, maka perlu dibutuhkan sistem pendukung keputusan dalam menentukan siswa berprestasi menggunakan metode SAW. Metode ini dapat menghasilkan data terbaik karena dilakukan dengan mencari kriteria dan nilai bobot dari setiap atribut, yang diharapkan dapat membantu pihak sekolah dalam melakukan proses penilaian siswa berprestasi sehingga hasil yang didapat lebih efektif dan efisien. Beberapa kriteria tersebut diantaranya poin pelanggaran, poin prestasi, kehadiran serta sikap dan perilaku. Setelah kriteria ditentukan, maka dibuatnya sistem pendukung keputusan untuk menentukan siswa berprestasi. Pengembangan sistem menggunakan Waterfall yang terdiri dari analisa kebutuhan, desain sistem, implementasi, pengujian dan proses perbaikan. Hasil dari perancangan sistem tersebut berupa perhitungan kriteria dan bobot sehingga menghasilkan matriks keputusan, matriks ternormalisasi sampai hasil dari perhitungan pada metode SAW.
The Time-Dependent Effect of Assistance on Peritoneal Dialysis Duration: An Analysis of Data from the RDPLF
Authors: Boyer Annabel , Lanot Antoine , Ficheux Maxence , Guillouet Sonia , Bechade Clémence , Lobbedez Thierry 
Published: October 2024

Background:
Peritoneal dialysis (PD) patient compliance is crucial for the prevention of complications. Assistance is associated with a lower risk of transfer to hemodialysis (HD). As the risk of noncompliance increases over time, the protective effect of assistance on the risk of transfer to HD may not be immediate after PD initiation but rather may appear after some time on PD. We aimed to analyze the time-varying effect of assistance on the risk of PD cessation.

Methods:
This retrospective study was conducted using data from the French Language PD Registry of incident PD patients between 2002 and 2018. Because of nonproportional hazards, with a change in the effect of the assistance modality on the different outcomes appearing at six months after PD initiation, the associations between the assistance modality and the different outcomes were explored using time-dependent coefficient Cox regression.

Results:
The study included 15675 patients. 6717 deaths, 4973 transfers to HD and 3065 kidney transplantations occurred. Both patients receiving nurse- and family-assisted PD had a lower risk of transfer to HD (mean cs-HR 0.67, 95% CI 0.62-0.72 and mean cs-HR 0.75, 95% CI 0.67-0.84). In the first six months after PD initiation, nurse-assisted PD patients had a greater risk of transfer to HD (<6 months cs-HR 1.18, 95% CI 1.03-1.36) but had a lower likelihood afterward (≥6 months cs-HR 0.57, 95% CI 0.53-0.62). Family-assisted PD was not associated with the risk of transfer to HD in the first 6 months after PD initiation, and those patients had a lower risk of transfer to HD afterward (≥6 months cs-HR 0.72, 95% CI 0.63-0.82).

Conclusions:
When implementing a national nurse-assisted PD program, its positive impact on PD duration should not be expected immediately after PD initiation. Assisted PD programs should be sustainable for at least 6 months to observe their benefits.