bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome-wide identification and transcript analysis of TCP transcription factors in grapevine
Authors: leng xiangpeng , Wei Hongru , Xu Xiaozhao , Ghuge Sandip A. , Jia Dongjie , Liu Gengsen , Wang Yongzhang , Yuan Yongbing 
Published: September 2019

Abstract
Background The plant-specific TCP transcription factors play different functions in multiple processes of plant growth and development. TCP family genes have been identified in several plant species, but no comprehensive analysis of the TCP family in grapevine has been undertaken to date, especially their roles in fruit development. Results A total of 18 non-redundant grapevine TCP (VvTCP) genes distributing on 11 chromosomes were identified. Phylogenetic and structural analysis showed that VvTCP genes were divided into two main classes - class I and class II. The Class II genes were further classified into two subclasses, the CIN subclass and the CYC/TB1 subclass. Segmental duplication was a predominant duplication event which caused the expansion of VvTCP genes. The cis-acting elements analysis and tissue-specific expression patterns of VvTCP genes demonstrated that these VvTCP genes might play important roles in plant growth and development. Expression patterns of VvTCP genes during fruit development and ripening were analyzed by RNA-Seq and qRT-PCR. Among them, eleven VvTCP genes were down-regulated during different fruit developmental stages, while only one VvTCP genes were up-regulated, suggesting that most VvTCP genes were probably related to early development in grapevine fruit. Futhermore, the expression of most VvTCP genes can be inhibited by drought and waterlogging stresses. Conclusions Our study establishes the first genome-wide analysis of the grapevine TCP gene family and provides valuable information for understanding the classification and functions of the TCP genes in grapevine.
Development of an interactive, web-based shared care plan to facilitate the follow-up care of colorectal cancer patients
Authors: Taggart Jane , Chin Melvin , Liauw Winston , Dolezal Alex , Plahn John , Harris Mark Fort 
Published: September 2019

Abstract
Introduction
A shared model of care between cancer services and primary health care is safe and acceptable and can address the increasing demands on cancer services for long term follow-up. This paper describes the challenges developing an interactive shared care plan for colorectal cancer follow-up care that supports collaboration between the care team and patient.

Methods
A systematic literature review was undertaken to identify web-based care plans for follow-up cancer care that would inform the implementation of a system to share an individualised care plan. We conducted individual consultations with 25 key informants/stakeholders to understand the requirements and challenges of establishing the web-based care plan and to identify the technical options to share the care plan. This was followed by a structured group consultation with 13 key stakeholders to obtain agreement on the model of care and the technical solution to share the care plan.

Results
We identified five web-based shared care plans for cancer follow-up care. These systems supported the creation and/or access to view or share the care plan via internet log-in, email or hard copy. None of the SCP had interactive functionalities to support collaboration and none of the included papers reported formalised models of shared care between cancer services and primary care.
The challenges identified included the security, privacy and sharing of patient information between public health services and primary health care and poor integration of clinical information systems. A primary care practitioners’ care planning system was selected for the pilot as it addressed the challenge of sharing clinical information between the public health system and external clinicians, partially addressed integration (integrated with the primary care clinical information systems but did not integrate with the cancer centre systems), supported collaboration between the care team and patient and was relatively inexpensive.

Conclusion
Primary care shared care planning systems seem a suitable option for sharing clinical information between a cancer service and primary care practitioners. We plan to evaluate the feasibility and acceptability of this interactive shared care plan to support shared cancer follow-up care.
The University of Limerick Education and Research Network for General Practice (ULEARN-GP): practice characteristics and general practitioner perspectives
Authors: O'Regan Andrew , Hayes Peter , O'Connor Ray , Casey Monica , O'Dwyer Patrick , Culhane Aidan , O'Donnell Patrick , Stack Gary , Cuddihy John , O'Connell Billy , O'Flynn Jerry , Cullen Walter , O'Doherty Jane , O'Connell Maurice , Glynn Liam 
Published: September 2019

Abstract
Background A well-functioning General Practice sector that has a strong research component is recognised as a key foundation of any modern health system. General practitioners (GPs) are more likely to collaborate in research if they are part of an established research network. The primary aims of this study are to describe Ireland’s newest general practice-based research network and to analyse the perspectives of the network’s members on research engagement. Method A survey was sent to all GPs participating in the network in order to document practice characteristics so that this research network’s profile could be compared to other national profiles of Irish general practice. In depth interviews were then conducted and analysed thematically to explore the experiences and views of a selection of these GPs on research engagement. Results All 134 GPs responded to the survey. Practices have similar characteristics to the national profile in terms of location, size, computerisation, type of premises and out of hours arrangements. Twenty-two GPs were interviewed and the resulting data was categorised into subthemes and four related overarching themes: GPs described catalysts for research in their practices, the need for coherence in how research is understood in this context, systems failures, whereby the current health system design is prohibitive of GP participation and aspirations for a better future. Conclusion This study has demonstrated that the research network under examination is representative of current trends in Irish general practice. It has elucidated a better understanding of factors that need to be addressed in order to encourage more GPs to engage in the research process.
More that unites us than divides us? A qualitative study of integration of community health and social care services
Authors: Mitchell Claire , Tazzyman Abigail , Howard Susan J. , Hodgson Damian 
Published: September 2019

Abstract
Background The integration of community health and social care services has been widely promoted nationally as a vital step to improve patient centred care, reduce costs, reduce admissions to hospital and facilitate timely and effective discharge from hospital. The complexities of integration raise questions about the practical challenges of integrating health and care given embedded professional and organisational boundaries in both sectors. We describe how an English city created a single, integrated care partnership organisation, to fully integrate community health and social care services. This led to the development of twelve integrated neighbourhood teams, combining and co-locating professionals across three separate localities. The aim of this research is to identify the context and the factors enabling and hindering integration from a qualitative process evaluation.Methods Twenty-four semi-structured interviews were conducted with equal numbers of health and social care staff at strategic and operational level. The data was subjected to thematic analysis.Results We describe three key themes: 1) shared vision and leadership; 2) organisational factors; 3) professional workforce factors. We found a clarity of vision and purpose of integration throughout the partnership organisation, but there were challenges related to the introduction of devolved leadership. There were widespread concerns that the specified outcome measures did not capture the complexities of integration. Organisational challenges included a lack of detail around clinical and service delivery planning, tensions around variable human resource practices and barriers to data sharing. A lack of understanding and trust meant professional workforce integration remained a key challenge, although integration was also seen as a potential solution to engender relationship building.Conclusions Given the long-term national policy focus on integration this ambitious approach to fully integrate community health and social care has highlighted implications for leadership, organisational design and inter-professional working. Given the ethos of valuing the local assets of individuals and networks within the new partnership organisation we found the integrated neighbourhood teams could all learn from each other. Many of the challenges of integration could benefit from embracing the inherent capabilities across the integrated neighbourhood teams and localities of this city.
How were situations of preventive and curative care expenditure for AIDS and medical burden of patients? Research based on "System of Health Accounts 2011 "
Authors: Zhan Huan , Wu Qiong , Zang Shuang , Zhou Liangrong , Wang Xin 
Published: September 2019

Abstract
Background The problem of AIDS response has not only involved public health, but also had a great impact on the family burden.The objective of this study was to estimate Hunan Province preventive and curative care expenditure(PCE) of AIDS in 2017 based on System of Health Accounts 2011 by quantity,financing scheme,health provider,health function,and to analyse the factors affecting patients' medical burden.Methods Through stratified multi-stage sampling method, 1336 institutions were surveyed to obtain AIDS prevention and control data, and combined with official data of Hunan Health Statistical Yearbook, Health Financial Yearbook and Government Input Monitoring System to estimate the AIDS PCE under the framework of SHA2011.Univariate analysis and ordered logistic regression were used to evaluate the factors affecting the medical burden of AIDS patients.Results The AIDS PCE of Hunan in 2017 was 266.67 million, mainly flowed to hospitals and disease prevention and control institutions. The proportions of curative care expenditure(CCE) and prevention expenditure were 51.39%, 48.61% respectively. From the point of view of the allocation of prevention expenditure, AIDS prevention methods were relatively traditional, and oral PrEP was not included in the national plan. All prevention expenditure and 88.52% of CCE were borne by public financing scheme.Family health expenditure accounted for 11.21% of CCE. None insurance, co-infection and length of stay are risk factors to the total expenditure of hospitalization and out-of-pocket health expenditure(all p <0.05,OR>1).Taking the age group under 30 as the reference, the partial regression coefficient of the age group over 60 was statistically significant ( OR (Totalexp) =1.809, OR (OOP) =0.30).Conclusions The financing structure of the Hunan Province PCE of AIDS was relatively stable and the flow of institutions was reasonable. The functional flow of expenditure embodied the principle of "prevention first". China should incorporate oral PrEP into the national guidelines as soon as possible to improve the allocation efficiency of AIDS prevention resources. Meantime, several measures should be taken to reduce the hospitalization expenses and medical burden of AIDS patients, including adjusting insurance compensation measures, increasing the rate of patients participating in insurance,encouraging commercial insurance to join the AIDS insurance system,and controlling length of stay in hospital.
Direct qPCR is a sensitive approach to detect Mycoplasma contamination in U937 cell cultures
Authors: Baaity Zain , Breunig Sven , Önder Kamil , Somoyvári Ferenc 
Published: September 2019

Abstract
Objective In order to make the Mycoplasma contamination monitoring task easier, we optimized a commercially available quantitative PCR (qPCR)-based detection kit to detect Mycoplasma DNA without DNA purification in a U937 suspension cell culture. We compared the sensitivities of the direct qPCR and the qPCR with the purified DNA template.

Results Our findings indicate that qPCR worked optimally with a 6 ml sample volume and a 52 o C annealing-extension temperature. We were able to decrease the annealing-extension step time from 60 sec to 20 sec without any major decrease in the reaction sensitivity. The total cycle time of the optimized direct qPCR was 65 minutes. The optimized qPCR protocol was used to detect Mycoplasma DNA directly and after DNA purification. Our findings indicate that the direct qPCR had a higher sensitivity compared to the regular qPCR. The Ct levels produced by the direct qPCR with 6 ml templates were almost identical to the Ct levels produced by the regular qPCR with DNA purified from a 60 ml cell culture sample (23.42 vs 23.49 average Ct levels respectively). The optimized direct qPCR protocol was successfully applied to monitor the elimination of Mycoplasma contamination from the U937 cell cultures.
Echocardiographic Assessment of Asymptomatic US Air Force Members with Early HIV Infection
Authors: Alvarado Gadiel Rafael , Usry Courtney , Gore Rosco , Watts James , Okulicz Jason 
Published: September 2019

Abstract
Objective

People living with HIV (PLHIV) are at increased risk for cardiovascular disease (CVD) and development of subclinical echocardiographic abnormalities. The pathogenicity of HIV induced cardiotoxicity has been described however the time to development of echocardiographic abnormalities after HIV acquisition remains unclear. In this study we describe the echocardiographic evaluations of asymptomatic US Air Force members who were diagnosed with HIV infection.

Results

Patients (n=50) were predominantly male (96%), mostly black (60%), with a mean age of 28 years. At HIV diagnosis, the mean viral load was 112,585 copies/mL and CD4 count was 551 cells/uL; 2 patients were diagnosed with AIDS. All were found to have normal systolic ejection fraction (EF) and global longitudinal strain (GLS) however evidence of right ventricular dilatation and cardiac remodeling was observed in 7 (14%) and 13 (26%) patients, respectively. Subgroup analyses showed no significant differences in echocardiographic findings by HIV disease severity or CVD risk factors (p >0.05 for all). This study suggests that untreated HIV may have a low impact on the development of echocardiographic abnormalities shortly after seroconversion. Longitudinal studies are warranted to determine the optimal CVD risk assessment strategies for PLHIV.
Coronary heart disease mortality is decreasing in Argentina and Colombia, but keeps increasing in Mexico: a time trend study
Authors: Arroyo-Quiroz Carmen , Barrientos-Gutierrez Tonatiuh , O'Flaherty Martin , Guzman-Castillo Maria , Mejia Lina Sofia Palacio , Saldarriaga Elkin de Jesus Osorio , Rodriguez Andrea Yanira Rodriguez 
Published: September 2019

Abstract
Background : Coronary heart disease (CHD) mortality rates have decreased in most countries but increased in low and middle-income countries. Few studies have analyzed CHD mortality trends in Latin America, specifically trends in young-adults and the effect of correcting these comparisons for nonspecific causes of death (garbage codes).Objective: To describe and compare standardized, age-specific, and garbage-code corrected mortality trends for CHD from 1985 to 2015 in Argentina, Colombia and Mexico. Methods: CHD deaths were grouped by country, year of registration, sex and 10-year age bands to calculate age-adjusted and age and sex specific rates for adults aged ≥25. We corrected for garbage-codes using the Global Burden of Disease methodology. Finally, we fitted Joinpoint regression models.Results: In 1985, age-standardized mortality rates per 100,000 were 136.6 in Argentina, 160.6 in Colombia and 87.51 in Mexico. Compared to 2015, mortality fell in Argentina and Colombia (51% and 6.5% respectively) and increased by 61% in Mexico. The steepest decline was observed in Argentinian women, and the sharpest increment in Mexican men. There has been an upward trend in young Mexicans since 1985. Garbage-code corrections produced increases in mortality rates, particularly in Argentina: approximately 80 additional deaths per 100,000 (14 in Colombia and 13 in Mexico). Conclusions: Latin American countries are at different stages of the epidemic. The disease burdens are bigger after correcting for misclassification. Although CHD mortality is falling in Argentina, the modest falls in Colombia and substantial rises in Mexico highlight the region’s need for effective, population-wide prevention policies.
Current Status In Kedir City Towards 90-90-90 Unaids Target (A Case study of Pesantren 1 Health Center)
Authors: Ssekalembe Geofrey , Isfandiari Muhammad Atoillah 
Published: September 2019

Abstract
Introduction In 2016, UN Member States committed to reduce new HIV infections to fewer than 500,000 annually by 2020, a 75% reduction compared with 2010, reduce AIDS-related deaths to fewer than 500 000 globally as a means of ending AIDS by 2030. The main UNAIDS 2020 target is to ensure that 90% of the people living with HIV know their status, 90% of the people living with HIV (who know their HIV status as positive) are already on ART treatment, 90% of people on treatment are virally suppressed. The objective of the study is to determine the current situation towards the 2020 90-9-90 UNAIDS target and the factors associated with HIV viral load suppression at Pesantren 1 Health center Method The study was a cross sectional study at Pesantren 1 Health Center in Kediri city. The researcher collected secondary data at from the January 2018 till May 2019, carried in depth interviews, then determined the percentage of HIV positive patients that did a HIV test and received their results as Positive, the percentage of HIV positive patients that started ART treatment, the percentage of HIV positive patients that have viral load suppression from the viral load tests done. The researcher used the chi square analysis to determine the significant factors that may determine viral suppression that are already on ART treatment and then applied the binary logistic regression to determine the significant factors that determine viral suppression among the HIV positive patients that are already on ART treatment. Results The progress towards the 90-90-90 UNAIDS target was at 2.4%, 93.4%, 20.5%. The time taken by the HIV positive patient to start ART treatment from the time of confirmation of HIV positive and the time taken to take the first viral load test from the time when this patient started ART treatment were significantly influencing Viral load suppression. Conclusion There is need to scale up HIV case-detection capacity through creating awareness about HIV, HIV testing and counselling (HTC), reducing stigma and discrimination and also need to expand of the ART services so as to achieve the 90-0-90 UNAIDS target.
Exploring pregnant women interest and anxiety to use the Internet and their perception and trust toward online information
Authors: Ahmadian Leila , Khajouei Reza , Kamali Sudabeh , Mirzaee Moghaddameh 
Published: September 2019

Abstract
Background The Internet often represents a source of accessible information. The aim of this study was to examine pregnant women’s interest and anxiety when they use the Internet and also to determine the criteria by which they evaluated the correctness and reliability of the information on the Internet.Methods A descriptive study was conducted between April and July 2015. The pregnant women at the offices of obstetricians/gynecologists were invited to participate in the study by filling out a questionnaire.Results Of the 450 distributed questionnaires, 420 were completed and analyzed. The mean of interest and anxiety scores respectively was 12.21 and 6.69 out of 20. The most important criteria for judging the reliability and trustworthiness of a website were whether its information is continuously updated (69.2%), or it is recommended by a doctor, nurse or other health professionals (63.1%).Conclusions : Since the interest to a website lowers the anxiety of pregnant women to use it, it is recommended to consider pregnant women’s preferences and needs and follow user-centered approaches when designing websites. Medical content of the websites also should be carefully reviewed by a multidisciplinary board of medical specialists for the correctness and reliability of the information.
Cutaneous Leishmaniasis A Neglected Tropical Disease: Community Knowledge, Attitude And Practices In An Endemic Area, Northwest Ethiopia
Authors: Tamiru Helina Fikre , Mashalla Yohana James , Mohammed Rezika , Tshweneagae Gloria Thupayagale 
Published: September 2019

Abstract
Background
Cutaneous leishmaniasis is one of the neglected tropical diseases in the Ethiopian highlands and studies on assessment of knowledge, attitude and practice of the community in endemic areas are scanty. The study aimed to assess the knowledge, attitude towards cutaneous leishmaniasis and treatment seeking practices in people living in the endemic highlands areas in the Northwest, Ethiopia and provide evidence-based information to guide development of appropriate interventions to reduce the impact of cutaneous leishmaniasis on communities.
Methods
Quantitative cross-sectional study was conducted in cutaneous leishmaniasis endemic districts (woredas) using a semi structured questionnaires. Households were randomly selected according to probability proportional to size of households in each enumeration area. Systematic random sampling of eligible households was based on the number of households recorded during listing of households. Descriptive statistics was used to describe numerical data, organise and summarise the data in a manner that gave meaning to the numerical form. Frequency tables were used to show descriptive analysis and regression analysis was used to determine correlation between variables.
Results
Majority of respondents 321(78.7%) lived in rural areas, age ranged between 18 and 85 years and most were farmers. Illiteracy was high (47.6%) among respondents and majority 358(87.8%) had seen patients with CL. Less than quarter (21.6%) had heard about sand flies and knowledge on the peak transmission period was low (46.3%). About 192 (47.1%) of the respondents indicated disfiguring lesions were the major clinical presentations, less than half 55(27.5%) of urban residents believed CL was treatable compared to 145(72.5%) of rural residents (P < 0.001). Traditional medicines were indicated as best treatment option by 209(51.2%) compared to 114(27.9%) for modern treatment. Major factors influencing treatment options included accessibility to treatment facilities, distance and short duration of treatment. Participants expressed negative experiential attitude and perceived control towards modern treatment because of inaccessibility and distance from where modern treatment is provided.
Conclusion
Priority should be given to primary prevention and appropriate awareness campaigns on lesion recognition, prevention. Information on modern treatment should be intensified.