bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization of deoxyribonucleic methylation and transcript abundance of sex-related genes during tempera ture-dependent sex determination in Mauremys reevesii†
Authors: Dong Jinxiu , Xiong Lei , Ding Hengwu , Jiang Hui , Zan Jiawei , Nie Liuwang 
Published: July 2019

AbstractA number of genes relevant for sex determination have been found in species with temperature-dependent sex determination. Epigenetics play a key role in sex determination, but characterization of deoxyribonucleic acid methylation of sex-related genes on temperature-dependent sex determination remains unclear. Mauremys reevesii is a typical species with temperature-dependent sex determination. In this study, we analyzed the Cytosine Guanine (CpG) methylation status of the proximal promoters, the messenger ribonucleic acid expression patterns and the correlation between methylation and expression levels of Aromatase, Forkhead box protein L2, Doublesex and mab3-related transcription factor 1, sex-determining region on Y chromosome-box 9, and anti-Müllerian hormone, which are key genes in sex determination in other species. We also analyzed the expression level of genes that encode enzymes involved in methylation and demethylation. The expression levels of Aromatase and Forkhead box protein L2 at the female producing temperature were higher than those at the male producing temperature; the expression levels of Doublesex and mab3-related transcription factor 1, sex-determining region on Y chromosome-box 9, and anti-Müllerian hormone were higher at MPT. The expression of some genes involved in methylation and demethylation is significantly different between male producing temperature and female producing temperature. The expression of messenger ribonucleic acid of genes involved in deoxyribonucleic acid methylation and demethylation affected by temperature, together with other factors, may change the methylation level of the regulatory regions of sex-related genes, which may further lead to temperature-specific expression of sex-related genes, and eventually affect the differentiation of the gonads.
Circumscription of the Sibbaldia procumbens complex (Potentilleae: Rosaceae) in China based on evidence from simple sequence repeat markers and morphology
Authors: Zhang Huajie , Feng Tao , Landis Jacob B , Zhang Xu , Meng Aiping , Deng Tao , Sun Hang , Wang Hengchang 
Published: July 2019

AbstractIn this study we aimed to delimit the boundaries of the Sibbaldia procumbens species complex, integrating morphological, geographical and molecular data at the population level. In total, 246 individuals were tested with simple sequence repeat (SSR) markers, and individuals were classified into three clusters. Stable variation in the morphology of leaflets, episepal and achenes was found between individuals from eastern and western Tibet, and this corresponds to the observed genetic structure when K = 2. The combined analysis of morphology and SSRs suggests three species should be recognized in the complex in China (S. procumbens, S. cuneata and S. aphanopetala). The ranges of these three species are mostly geographically separate from each other. Sibbaldia aphanopetala has often been treated as a variety of S. procumbens, but here we restore it to species rank.
Constitutional Protection of Children’s Rights: Visibility, Agency and Enforceability
Authors: O’Mahony Conor 
Published: July 2019

Abstract
While almost every state in the world has ratified the United Nations Convention on the Rights of the Child, there is less consensus around the manner in which the rights protected by it should be protected in national constitutions. To say that a constitution makes provision for children’s rights is just a starting point: the extent to which a national constitution takes a genuine child rights approach will depend on the quality of the constitutional provisions in question. This article aims to provide a typology which can be used to assess whether the approach taken by any given constitution to the protection of children’s rights is in line with the child rights approach envisaged by the Convention by analysing individual constitutions along three separate spectrums. The Visibility spectrum measures how visible children are in a constitutional scheme; the Agency spectrum measures the extent to which children are considered to be independent, autonomous rights holders; and the Enforceability spectrum measures the extent to which children’s constitutional rights can be enforced.
The rule of law and maritime security: understanding lawfare in the South China Sea
Authors: Guilfoyle Douglas 
Published: July 2019

Abstract
Does the rule of law matter to maritime security? One way into the question is to examine whether states show a discursive commitment that maritime security practices must comply with international law. International law thus provides tools for argument for or against the validity of certain practices. The proposition is thus not only that international law matters to maritime security, but legal argument does too. In this article, these claims will be explored in relation to the South China Sea dispute. The dispute involves Chinese claims to enjoy special rights within the ‘nine-dash line’ on official maps which appears to lay claim to much of the South China Sea. Within this area sovereignty remains disputed over numerous islands and other maritime features. Many of the claimant states have engaged in island-building activities, although none on the scale of China. Ideas matter in such contests, affecting perceptions of reality and of what is possible. International law provides one such set of ideas. Law may be a useful tool in consolidating gains or defeating a rival's claims. For China, law is a key domain in which it is seeking to consolidate control over the South China Sea. The article places the relevant Chinese legal arguments in the context of China's historic engagement with the law of the sea. It argues that the flaw in China's approach has been to underestimate the extent to which it impinges on other states' national interests in the maritime domain, interests they conceptualize in legal terms.
13 Effects of bismuth subsalicylate and calcium-ammonium nitrate on in vitro fermentation of a high-concentrate substrate
Authors: Mejia-Turcios Sebastian E , Stotz Miranda K , Osorio Andrea M , Urso Philip M , Jennings Thomas G , Espinoza Nadira , Kondratovich Lucas , Carvalho Luis F , Sandes Michael D , Sarturi Jhones O , DiLorenzo Nicolas , Crossland Whitney , Henry Darren D 
Published: July 2019

Abstract
An experiment was performed to determine the effects of bismuth subsalicylate (BSS) and calcium-ammonium nitrate (CAN) on in vitro fermentation of a high-concentrate (87% concentrate, DM basis) substrate. Serum bottles containing 20 mL of a 2:1 buffer:ruminal fluid inoculum and 0.2 g of substrate were incubated for 24 h. Four ruminally cannulated steers (BW = 520 ± 30 kg) were used as ruminal fluid donors and each donor was considered a block. Treatments were arranged in a 2 × 2 factorial with the following factors: BSS (0 or 0.33%, DM basis) and CAN (0 or 2.22%, DM basis). Treatments were made isonitrogenous with urea. In vitro organic matter digestibility (IVOMD) was determined in separate 100-mL centrifuge tubes. Data were analyzed using the MIXED procedure of SAS with the fixed effect of BSS, CAN, BSS × CAN, and the random effect of donor. An interaction (P < 0.01) was observed for total gas production (TGP). When CAN was included, without BSS, TGP was increased (P < 0.01); however, the combination of CAN with BSS did not affect (P = 0.85) TGP when compared to the combination of urea and BSS. Ammonia-N tended (P = 0.10) to increase when CAN was used as N source rather than urea. In vitro OM digestibility (P > 0.23) and final pH (P > 0.66) of in vitro ruminal fermentation were not affected by treatments. A tendency (P = 0.06) for an interaction regarding the production of H2S was observed; however, there were no treatment mean differences (P > 0.28). The combination of CAN and BSS did not negatively affect in vitro fermentation parameters such as OM digestion and gas production; however, a reduction in H2S with the combination of BSS and CAN may indicate potential benefits of such feeding strategies for feedlot cattle
10 Finishing beef cattle under cover reduces heat load and improves efficiency during the summer
Authors: Sims William M , Stewart Lawton , Segers Jacob R , McKee Robert W , Rigdon Macc , Thomas Chevise L , Stelzleni Alexander M 
Published: July 2019

Abstract
Heat-stress in finishing cattle presents a significant risk to efficiency and economic viability. The project objective was to quantify the effects of long-term heat stress when finishing cattle during the summer in the southeastern United States. Forty-five Angus crossbred steers (446±23 kg) were blocked by weight and randomly assigned to environmental finishing treatments including: covered with fan (CWF), covered without fan (CNF), or outside without shade (OUT). For 92 d steers were individually fed a typical feedlot ration. Environmental data were continuously recorded including: black globe temperature (BG), heat load index (HLI), and accumulated heat load units (AHLU). Feed intake was recorded daily, and steers were weighed every 20–25 days. When the first treatment averaged 613-kg all steers were slaughtered, and carcass data were collected. Data were analyzed as a Mixed Model (JMP V13; SAS Inst.) and means were separated (Least Squares Means). Average maximal BG was lower for covered finishing than OUT (P < 0.01) however for HLI CWFCNF>OUT, while G:F was similar (P = 0.22) between CWF and CNF, which were greater (P < 0.01) than OUT. Hot carcass weights were heavier for CWF than OUT (P < 0.01) and CNF was similar to both (P ≥ 0.11). There was no difference for USDA Yield Grade (2.6; P = 0.44), or marbling score (Modest20; P = 0.76). Steers finished under cover were more efficient than steers finished in open dry-lots. The addition of cooling fans further improved steer gains over those that were covered without fans.
9 Reduce Reuse ReRumen: Variability in nutritive value of paunch manure
Authors: Garcia Taylor J , Brady Jeffrey A , Guay Kimberly A , Muir James P , Smith William B 
Published: July 2019

Abstract
Ruminant animals develop a diverse and complex microbial ecosystem for digesting fibrous feedstuffs, but this material represents a cost to the industry when bound for rendering at harvest. Our objective was to determine the feed value of paunch manure recovered from an abattoir. Twelve paunch samples were collected from slaughtered cattle at the Tarleton State University Meat Laboratory, Stephenville, TX. Samples were dried under forced air at 55°C to a constant weight and ground to pass through 2-mm screen. A subsample was ground to 1-mm and assayed for DM, CP, and sequential NDF and ADF. Physically effective fiber (peNDF) was calculated with 2 L of unground, dried at 55 ˚C, paunch material using the manually-operated Penn State Particle Separator. Paunch peNDF was determined by multiplying the physical effectiveness factor by the total NDF content of the sample. Samples (2-mm) were subject to batch culture in vitro digestibility assays for determination of digestibility coefficients. Data were analysed as a random effects model using PROC GLIMMIX in SAS. Neutral detergent fiber, ADF, ADL, CP, and peNDF values were 68.1, 39.9, 10.9, 15.0, and 38.7%, respectively. Contribution to variance of the individual animal for NDF, ADF, ADL, CP, and peNDF was 97.2, 97.9, 95.4, 19.1, and 97.5%, respectively. In vitro true digestibility (IVTD) and in vitro neutral detergent fiber digestibility (IVNDFD) was recorded as 46.2% and 21.6%, respectively. Contribution to variance of the animal for IVTD and IVNDFD was 99.05 and 98.49%, respectively. Data collected suggest paunch manure could provide valuable nutrients for livestock production, specifically fiber and protein. For full viability of application in a sustainable system, a centralized receiving and compositing system may be useful for development of a consistent product.
4 Impact of potassium application and harvest regime in alfalfa yield, forage quality and stand persistence in South Georgia
Authors: Thinguldstad Britta , Tucker Jennifer J , Baxter Lisa , Segers Jacob R 
Published: July 2019

Abstract
Potassium fertilization and harvest timing can both impact stand life of alfalfa (Medicago sativa L.). In the Southeast, high temperatures and humidity often influence harvest timing and fertilization. Current nutrient management and harvest recommendations are generated from research conducted in northern climates with different varieties. The objective of this trial is to determine the impact of potassium fertilization and harvest regime on stand persistence, forage yield, and quality of ‘Bulldog 805’ alfalfa in the Southeast. This study was conducted at the Coastal Plain Experiment Station in Tifton, GA, on a two-year-old stand of ‘Bulldog 805’ alfalfa planted fall 2015. Plots were randomly assigned using a split block design. Main plots were harvest treatment: bud, and 10, 30, and 50% bloom stage. Plots were subdivided to examine K fertilization at rates: 0, 67, 101, 134, and 168 kg/ha, split 3 times throughout the season. Plots were visually assessed each harvest to determine ground cover and percent bloom. Fifty shoots were collected to evaluate leaf:stem and mass:shoot ratio, and yield was determined through harvest using a Swift forage harvester. Grab samples were collected for nutritional analysis using near-infrared reflectance spectroscopy. Data was analyzed using the PROC GLIMMIX of SAS with repeated measures analysis option at a = 0.05. Leaf:stem ratio was not significant for all main effects (P > 0.46). Harvest treatment had an effect on yield, with 10% bloom greater than bud stage (1,321 kg/ha and 1,104 kg/ha, respectively; P = 0.04). Crude protein levels were the greatest in bud stage and lowest with 50% bloom (22.8% and 21.2%; P < 0.01). Total digestible nutrients were greater in bud and 10% bloom compared to 30% and 50% (65% and 63%, respectively; P < 0.01). Potassium application and harvest regime can impact alfalfa growth. Funding for this project was provided by USDA-NIFA-AFRP grant #2016-70005-25653.
1 The effect of breeding system on voluntary forage consumption of beef cows
Authors: Andresen Claire , Holder Amanda , Gross Megan , Wiseman Aksel , McGee Adam , Lalman David 
Published: July 2019

Abstract
Recent genetic trends in beef breeds suggest an imbalance in the selection of output and input traits. The inverse relationship between cow maintenance requirements with output traits such as growth and milk production may have ramifications on carrying costs of cows as well as feed intake of the cow herd. One method commonly used to maximize efficiency within a production system is to utilize a crossbreeding system to capitalize on breed complementarity. The objective of this study was to investigate the impact of breeding system on forage intake and determine correlations between breeding system, dry matter intake, and maintenance requirements of beef cows. To do this, a 45-d feeding trial was conducted using 59 pregnant, non-lactating Angus (ANG; n = 32) and Hereford x Angus (HA; n = 27) cows similar in mature BW, growth, and milk potential. Animals were assigned to pen based on breed with four pen replicates per breed. Cows were housed in dry-lot pens equipped with shade, windbreaks, and feed bunks with free-choice access to clean water. A chopped hay ration (65% DM, 55.7% TDN, 7.8% CP) was provided ad libitum to determine forage intake. Daily intake tended to be lower (P = 0.06) for HA compared to ANG (134.25 g/kg MBW ANG; 125.37 g/kg MBW HA). There was no difference in BW at initiation or conclusion of the trial, however, HA sustained greater BCS throughout the duration of the study (P > 0.01). After accounting for differences in retained energy, there was no detectable differences in maintenance requirements (P = 0.11). Results of this study suggest lower forage intake of crossbred cattle and suggests potential differences in energy prioritization and partitioning between breeds.
26 Effects of experimentally-induced hypoxemia on hemodynamics and blood gases, and the performance of pulse oximeters in cattle
Authors: Fontenot Lauren R , Carstens Gordon E , Lepiz Mauricio , Washburn Kevin , Hardy Joanne 
Published: July 2019

Abstract
Arterial oxyhemoglobin saturation (SaO2) is considered to be the reference method for evaluating lung function. There is current interest in development of biosensors to measure SpO2 (peripheral oxygen saturation) using infrared technology as a non-invasive alternative for pre-clinical respiratory detection of disease in animals. Objectives of this study were to investigate effects of experimentally-induced hypoxemia on hemodynamics (heart rate, HR and blood pressure, BP) and SaO2, and to evaluate the ability of 2 SpO2 pulse oximeters (PowerLab, AD Instruments; Passport2, Datascope) to predict SaO2 in cattle. Further, arterial lactate concentrations were measured as an indicator of oxygen delivery to tissues. Graded levels of hypoxia in seven anesthetized Holstein steers (BW = 127 ± 7 kg) were achieved by step-wise reductions in inspired oxygen fraction (FiO2) from baseline (20–35%) to target levels of 14–15%, 16–17%, and 18–19%. When the desired FiO2 levels were sustained for at least 3 min, arterial blood samples (n = 56) were collected and analyzed using a co-oximeter (pHOx-Ultra, Nova Biomedical) to determine SaO2 and lactate. Simultaneously, BP, HR and SpO2 data from the 2 pulse oximeters were recorded. Data were analyzed with a mixed model that included level of FiO2 as fixed effect. As expected, SaO2 decreased (P < 0.001) as FiO2 was reduced. Heart rate increased (P < 0.001) as FiO2 was reduced, although BP was unaffected by hypoxia. While not significantly different (P = 0.15), arterial lactate concentrations were reduced with declining FiO2. Likewise, the SpO2 values recorded by both pulse oximeters decreased (P < 0.001) incrementally as FiO2 declined, although values were only moderately correlated (P < 0.01; r = 0.35 to 0.41) with SaO2. These results demonstrate that future efforts to develop biosensors to monitor SpO2 and HR may have utility for preclinical detection of respiratory disease in livestock.
84 Cooking loss of ground beef
Authors: Coombs Robin G , Richardon C R 
Published: July 2019

Abstract
This study was conducted to measure two quality variables of ground beef: cooking loss and shrinkage. Twenty 454g packages of ground chuck from a commercial grocery store 80:20 lean:fat ratio (calculated per label to be 71:29), and 20 packages of ground beef from a specific breed (SB) (calculated per label to be 75:25) were used. Each package was divided into four hand-formed patties weighing 118-120g (n = 80). Prior to grilling, the patties were weighed, circumference measured (cm), and thickness measured (cm). Patties were grilled on a George Foreman Grill to an internal temperature of 73.9° C. Cooking loss (meat drippings) from grilled patties was collected. After reaching the desired internal temperature, individual patties were removed from the grill, weighed, and circumference and thickness measured. Cooking loss was collected in a grease tray and from the grill surface with a spatula. Cooking loss was weighed (g) and contents poured into a glass jar and stored in a freezer for further evaluation. There was no difference (P > 0.05) in cooking loss between the control and SB treatment, 61.40% and 61.34% respectively. A difference (P < 0.05) was found in cooking shrinkage (circumference and thickness). Circumference between fresh and cooked showed a change of 15.48% (control) and 12.88% (SB) (P < 0.05). Patty thickness between fresh and cooked changed by 9.32% (control) and 5.71 % (SB) (P<0.05). Total cooking loss per 454g package did not differ 13.74% (control) and 14.16% (SB). However, when cooking loss was separated in solid and liquid portions, the solid portion was 19.71% (control) and 28.30% (SB) (P < 0.05). These data indicate that quality attributes of ground beef vary between sources with similar lean:fat ratio.
81 Species-specificity in metmyoglobin reduction
Authors: Bechtold Emily , Suman Surendranath , Mohanty Smita , Mazumder Suman , Krishnan Sadagopan , Nerimetla Raja 
Published: July 2019

Abstract
Myoglobin is the primary sarcoplasmic protein responsible for meat color. Previous research has reported that myoglobin oxidation is species-specific. Metmyoglobin reducing activity is an inherent property to limit myoglobin oxidation. However, limited research has determined species specificity in metmyoglobin reducing properties. The objective of current study was to compare metmyoglobin reducing properties of eight different species such as beef, porcine, bison, deer, emu, equine, goat, and sheep in vitro. Myoglobin was isolated from eight different species via ammonium sulfate precipitation. The pH of the myoglobin was adjusted by passing through a column pre-calibrated with 50 mM phosphate buffer at pH 5.6. All species myoglobin were converted to metmyoglobin, and the metmyoglobin reduction was determined by two different approaches, non-enzymatic metmyoglobin reducing activity (NMRA) and oxidation-reduction potential (ORP). In the first method, NADH (electron donor), EDTA, and methylene blue (electron carrier), were added in a cuvette and increase in absorbance at 582 nm was monitored using a UV-Vis spectrophotometer. In the second method, the ability of the heme to get reduced was determined using an RedoxSys analyzer, in which electron was directly transferred to heme. The NMRA and ORP experiments were replicated five times. The data were analyzed using the Mixed Procedure of SAS, with species as the fixed effect. There were species-specific differences (P < 0.05) in NMRA and ORP. Bovine myoglobin had the greatest (P < 0.05) NMRA compared with sheep, equine, goat, deer, bison, pork, and emu. There were no differences (P > 0.05) in NMRA among equine, goat, deer, bison, pork, and emu. ORP studies indicated that beef and porcine myoglobins had the greatest ability to get reduced (P < 0.05) compared with other species. Hence, use of different techniques and approaches will help to elucidate the mechanistic basis of metmyoglobin reduction.