bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cross-level impact of team goal orientation and individual goal orientation on individual creativity
Authors: Zhang Jian , Ji Ming , Anwar Ch. Mahmood , Li Quanxi , Fu Guanghua 
Published: February 2018

AbstractThis study explored how individual and team-level goal orientation influence individual creativity in a work setting. By creating a cross-level theoretical framework, we tested 562 members of 81 teams currently working in various companies in China. The study analyzed the relationships between individual goal orientation, team goal orientation, and individual creativity from cross-level perspective linked by motivated information processing theory. Applying multilevel research method and hierarchical-level modeling, we found that team learning goal orientation and individual learning goal orientation influence individual creativity through different information activities at their own levels. Moreover, team learning goal orientation has a positive and team performance-avoidance goal orientation has a negative effect on individual creativity through team information exchange, while individual learning goal orientation poses a positive effect on individual creativity through individual information elaboration. In conclusion, it was indicated that team members tend to elaborate more work-related information when teams are more learning-orientated. Conversely, team members do not tend to elaborate information when their team has higher performance-avoidance goal orientation.
Acoustic characteristics and learner profiles of low-, mid- and high-level second language fluency
Authors: SAITO KAZUYA , ILKAN MELTEM , MAGNE VIKTORIA , TRAN MAI NGOC , SUZUKI SHUNGO 
Published: February 2018

ABSTRACTIn the context of 90 adult Japanese learners of English with diverse second language experience and 10 native speakers, this study examined the linguistic characteristics and learner profiles of low-, mid- and high-level fluency performance. The participants’ spontaneous speech samples were initially rated by 10 native listeners for global fluency on a 9-point scale (1 =dysfluent, 9 =very fluent), and then divided into four proficiency groups via cluster analyses: low (n= 29), mid (n= 30), high (n= 31), and native (n= 10). Next, the data set was analyzed for the number of pauses within/between clauses, articulation rate, and the frequency of repetitions/self-corrections. According to the results of a series of analyses of variance, the frequency of final-clause pauses differentiated low- and mid-level fluency performance; the number of mid-clause pauses differentiated mid- and high-level performance; and articulation rate differentiated high-level and nativelike performance. The analyses also found that the participants’ second language fluency was significantly associated with their length of residence profiles (0–18 years), but not with their age of arrival profiles (19–40 years).
Characteristics associated with mortality in patients with chronic obstructive pulmonary disease (COPD)–heart failure coexistence
Authors: Plachi Franciele , Balzan Fernanda M. , Sanseverino Renata A. , Palombini Dora V. , Marques Renata D. , Clausell Nadine O. , Knorst Marli M. , Neder J. Alberto , Berton Danilo C. 
Published: February 2018

AbstractAimTo investigate if cardiac/pulmonary functional tests and variables obtained from clinical practice (body mass index, dyspnea, functional class, clinical judgment of disability to perform an exercise test and previous hospitalization rate) are related to mortality in patients with overlap chronic obstructive pulmonary disease (COPD) and chronic heart failure (CHF).BackgroundAlthough the coexistence of COPD and CHF has been growingly reported, description of survival predictors considering the presence of both conditions is still scarce.MethodsUsing a cohort design, outpatients with the previous diagnosis of COPD and/or CHF that performed both spirometry and echocardiography in the same year were followed-up during a mean of 20.9±8.5 months.FindingsOf the 550 patients initially evaluated, 301 had both spirometry and echocardiography: 160 (53%) with COPD on isolation; 100 (33%) with CHF on isolation; and 41 (14%) with overlap. All groups presented similar mortality: COPD 17/160 (11%); CHF 12/100 (12%); and overlap 7/41 (17%) (P=0.73). In the overlap group (n=41), inability to exercise and hospitalization rate were the unique parameters associated with higher mortality (seven events) in univariate analyses. In conclusion, inability to exercise and hospitalization rate emerged as the unique parameters associated with mortality in our sample.
NAD+ analog reveals PARP-1 substrate-blocking mechanism and allosteric communication from catalytic center to DNA-binding domains
Authors: Langelier Marie-France , Zandarashvili Levani , Aguiar Pedro M. , Black Ben E. , Pascal John M. 
Published: February 2018

AbstractPARP-1 cleaves NAD+ and transfers the resulting ADP-ribose moiety onto target proteins and onto subsequent polymers of ADP-ribose. An allosteric network connects PARP-1 multi-domain detection of DNA damage to catalytic domain structural changes that relieve catalytic autoinhibition; however, the mechanism of autoinhibition is undefined. Here, we show using the non-hydrolyzable NAD+ analog benzamide adenine dinucleotide (BAD) that PARP-1 autoinhibition results from a selective block on NAD+ binding. Following DNA damage detection, BAD binding to the catalytic domain leads to changes in PARP-1 dynamics at distant DNA-binding surfaces, resulting in increased affinity for DNA damage, and providing direct evidence of reverse allostery. Our findings reveal a two-step mechanism to activate and to then stabilize PARP-1 on a DNA break, indicate that PARP-1 allostery influences persistence on DNA damage, and have important implications for PARP inhibitors that engage the NAD+ binding site.
Chiral DOTA chelators as an improved platform for biomedical imaging and therapy applications
Authors: Dai Lixiong , Jones Chloe M. , Chan Wesley Ting Kwok , Pham Tiffany A. , Ling Xiaoxi , Gale Eric M. , Rotile Nicholas J. , Tai William Chi-Shing , Anderson Carolyn J. , Caravan Peter , Law Ga-Lai 
Published: February 2018

AbstractDespite established clinical utilisation, there is an increasing need for safer, more inert gadolinium-based contrast agents, and for chelators that react rapidly with radiometals. Here we report the syntheses of a series of chiral DOTA chelators and their corresponding metal complexes and reveal properties that transcend the parent DOTA compound. We incorporated symmetrical chiral substituents around the tetraaza ring, imparting enhanced rigidity to the DOTA cavity, enabling control over the range of stereoisomers of the lanthanide complexes. The Gd chiral DOTA complexes are shown to be orders of magnitude more inert to Gd release than [GdDOTA]−. These compounds also exhibit very-fast water exchange rates in an optimal range for high field imaging. Radiolabeling studies with (Cu-64/Lu-177) also demonstrate faster labelling properties. These chiral DOTA chelators are alternative general platforms for the development of stable, high relaxivity contrast agents, and for radiometal complexes used for imaging and/or therapy.
Precision antimicrobial therapeutics: the path of least resistance?
Authors: Spaulding Caitlin N. , Klein Roger D. , Schreiber Henry L. , Janetka James W. , Hultgren Scott J. 
Published: February 2018

AbstractThe emergence of drug-resistant pathogens has led to a decline in the efficacy of traditional antimicrobial therapy. The rise in resistance has been driven by widespread use, and in some cases misuse, of antibacterial agents in treating a variety of infections. A growing body of research has begun to elucidate the harmful effects of broad-spectrum antibiotic therapy on the beneficial host microbiota. To combat these threats, increasing effort is being directed toward the development of precision antimicrobial therapeutics that target key virulence determinants of specific pathogens while leaving the remainder of the host microbiota undisturbed. This includes the recent development of small molecules termed “mannosides” that specifically target uropathogenic E. coli (UPEC). Mannosides are glycomimetics of the natural mannosylated host receptor for type 1 pili, extracellular appendages that promotes UPEC colonization in the intestine. Type 1 pili are also critical for colonization and infection in the bladder. In both cases, mannosides act as molecular decoys which potently prevent bacteria from binding to host tissues. In mice, oral treatment with mannosides simultaneously clears active bladder infection and removes intestinal UPEC while leaving the gut microbiota structure relatively unchanged. Similar treatment strategies successfully target other pathogens, like adherent-invasive E. coli (AIEC), an organism associated with Crohn’s disease (CD), in mouse models. While not without its challenges, antibiotic-sparing therapeutic approaches hold great promise in a variety of disease systems, including UTI, CD, otitis media (OM), and others. In this perspective we highlight the benefits, progress, and roadblocks to the development of precision antimicrobial therapeutics.
Freshwater transitions and symbioses shaped the evolution and extant diversity of caridean shrimps
Authors: Davis Katie E. , De Grave Sammy , Delmer Cyrille , Wills Matthew A. 
Published: February 2018

AbstractUnderstanding the processes that shaped the strikingly irregular distribution of species richness across the Tree of Life is a major research agenda. Changes in ecology may go some way to explain the often strongly asymmetrical fates of sister clades, and we test this in the caridean shrimps. First appearing in the Lower Jurassic, there are now ~3500 species worldwide. Carideans experienced several independent transitions to freshwater from marine habitats, while many of the marine species have also evolved a symbiotic lifestyle. Here we use diversification rate analyses to test whether these ecological traits promote or inhibit diversity within a phylogenetic framework. We demonstrate that speciation rates are more than twice as high in freshwater clades, whilst symbiotic ecologies are associated with lower speciation rates. These lower rates amongst symbiotic species are of concern given that symbioses often occur in some of the most diverse, delicately balanced and threatened marine ecosystems.
Advanced layout techniques for high‐speed analogue circuits in 28 nm HKMG CMOS process
Authors: Meng F. , Li K. , Thomson D.J. , Wilson P. , Reed G.T. 
Published: February 2018

The effect of optimising the transistor finger width on the performance of high‐speed analogue circuits in deep sub‐micron processes is investigated, demonstrated in a 28 nm high‐K/metal gate CMOS technology process. Silicon proven results demonstrate that the oscillator with a finger width of 440 nm gives the best performance based on the figure of merit (=142) among the benchmark design examples used.
Highly isolated crossed dipole antenna with matched copolar beams
Authors: Mirmozafari M. , Saeidi‐Manesh H. , Zhang G. 
Published: February 2018

A bent crossed dipole antenna for dual linear polarisation applications is introduced. The proposed efficient techniques incorporated into the design provide the antenna with high port isolation and low cross‐polarisation. A better than 56 dB measured port‐to‐port isolation is achieved in the frequency range of 2.7–3 GHz. The cross‐polarisation level remains 40 dB below the copolarisation peak in both principal planes. A pair of matched copolar beams in the corresponding principal planes is obtained owing to the use of identical and symmetrical structures in orthogonal directions. These features along with simple geometry and inexpensive PCB fabrication make the proposed antenna a promising candidate for multi‐mission applications.
Identifying two groups of massive stars aligned in thel~ 38° Galactic direction
Authors: Ramírez Alegría S. , Herrero A. , Rübke K. , Marín-Franch A. , García M. , Borissova J. 
Published: February 2018

Context.Recent near-infrared data have contributed to unveiling massive and obscured stellar populations in both new and previously known clusters in our Galaxy. These discoveries have lead us to view the Milky Way as an active star-forming machine.Aims.We look for young massive cluster candidates as over-densities of OB-type stars. The first search, focused on the Galactic directionl= 38°, resulted in the detection of two objects with a remarkable population of OB-type star candidates.Methods.With a modified version of the friends-of-friends algorithm AUTOPOP and using 2MASS and UKIDSS-GPS near-infrared (J,H, andK) photometry for one of our cluster candidates (named Masgomas-6) we selected 30 stars for multi-object and long-slitHandKband spectroscopy. With the spectral classification and the near-infrared photometric data, we derive individual distance, extinction, and radial velocity.Results.Of the 30 spectroscopically observed stars, 20 are classified as massive stars, including OB-types (dwarfs, giants and supergiants), two red supergiants, two Wolf−Rayets (WR122-11 and the new WR122-16), and one transitional object (the LBV candidate IRAS 18576+0341). The individual distances and radial velocities do not agree with a single cluster, indicating that we are observing two populations of massive stars in the same line of sight: Masgomas-6a and Masgomas-6b. The first group of massive stars, located at 3.9$^{&#x002B;0.4}_{-0.3}$ kpc, contains both Wolf−Rayets and most of the OB-dwarfs; the second group, located at 9.6 ± 0.4 kpc, hosts the LBV candidate and an evolved population of supergiants. We are able to identify massive stars at two Galactic arms, but we cannot clearly identify whether these massive stars form clusters or associations.
Global survey of star clusters in the Milky Way
Authors: Piskunov A. E. , Just A. , Kharchenko N. V. , Berczik P. , Scholz R.-D. , Reffert S. , Yen S. X. 
Published: February 2018

Context. The all-sky Milky Way Star Clusters (MWSC) survey provides uniform and precise ages, along with other relevant parameters, for a wide variety of clusters in the extended solar neighbourhood.
Aims. In this study we aim to construct the cluster age distribution, investigate its spatial variations, and discuss constraints on cluster formation scenarios of the Galactic disk during the last 5 Gyrs.
Methods. Due to the spatial extent of the MWSC, we have considered spatial variations of the age distribution along galactocentric radius RG, and along Z-axis. For the analysis of the age distribution we used 2242 clusters, which all lie within roughly 2.5 kpc of the Sun. To connect the observed age distribution to the cluster formation history we built an analytical model based on simple assumptions on the cluster initial mass function and on the cluster mass-lifetime relation, fit it to the observations, and determined the parameters of the cluster formation law.
Results. Comparison with the literature shows that earlier results strongly underestimated the number of evolved clusters with ages t ≳ 100 Myr. Recent studies based on all-sky catalogues agree better with our data, but still lack the oldest clusters with ages t ≳ 1 Gyr. We do not observe a strong variation in the age distribution along RG, though we find an enhanced fraction of older clusters (t > 1 Gyr) in the inner disk. In contrast, the distribution strongly varies along Z. The high altitude distribution practically does not contain clusters with t < 1 Gyr. With simple assumptions on the cluster formation history, the cluster initial mass function and the cluster lifetime we can reproduce the observations. The cluster formation rate and the cluster lifetime are strongly degenerate, which does not allow us to disentangle different formation scenarios. In all cases the cluster formation rate is strongly declining with time, and the cluster initial mass function is very shallow at the high mass end.
Suppressive Role of Tissue Factor Pathway Inhibitor-α in Platelet-Dependent Fibrin Formation under Flow Is Restricted to Low Procoagulant Strength
Authors: Thomassen Stella , Mastenbroek Tom , Swieringa Frauke , Winckers Kristien , Feijge Marion , Schrijver Roy , Cosemans Judith , Maroney Susan , Mast Alan , Hackeng Tilman , Heemskerk Johan 
Published: February 2018

AbstractTissue factor pathway inhibitor-alpha (TFPI-α) is a Kunitz-type serine protease inhibitor, which suppresses coagulation by inhibiting the tissue factor (TF)/factor VIIa complex as well as factor Xa. In static plasma-phospholipid systems, TFPI-α thus suppresses both factor Xa and thrombin generation. In this article, we used a microfluidics approach to investigate how TFPI-α regulates fibrin clot formation in platelet thrombi at low wall shear rate. We therefore hypothesized that the anticoagulant effect of TFPI-α in plasma is a function of the local procoagulant strength—defined as the magnitude of thrombin generation under flow, due to local activities of TF/factor VIIa and factor Xa. To test this hypothesis, we modulated local coagulation by microspot coating of flow channels with 0 to 100 pM TF/collagen, or by using blood from patients with haemophilia A or B. For blood or plasma from healthy subjects, blocking of TFPI-α enhanced fibrin formation, extending from a platelet thrombus, under flow only at <2 pM coated TF. This enhancement was paralleled by an increased thrombin generation. For mouse plasma, genetic deficiency in TFPI enhanced fibrin formation under flow also at 0 pM TF microspots. On the other hand, using blood from haemophilia A or B patients, TFPI-α antagonism markedly enhanced fibrin formation at microspots with up to 100 pM coated TF. We conclude that, under flow, TFPI-α is capable to antagonize fibrin formation in a manner dependent on and restricted by local TF/factor VIIa and factor Xa activities.
Progressive approach for SNP calling and haplotype assembly using single molecular sequencing data
Authors: Guo Fei , Wang Dan , Wang Lusheng 
Published: February 2018

AbstractMotivationHaplotype information is essential to the complete description and interpretation of genomes, genetic diversity and genetic ancestry. The new technologies can provide Single Molecular Sequencing (SMS) data that cover about 90% of positions over chromosomes. However, the SMS data has a higher error rate comparing to 1% error rate for short reads. Thus, it becomes very difficult for SNP calling and haplotype assembly using SMS reads. Most existing technologies do not work properly for the SMS data.ResultsIn this paper, we develop a progressive approach for SNP calling and haplotype assembly that works very well for the SMS data. Our method can handle more than 200 million non-N bases on Chromosome 1 with millions of reads, more than 100 blocks, each of which contains more than 2 million bases and more than 3K SNP sites on average. Experiment results show that the false discovery rate and false negative rate for our method are 15.7 and 11.0% on NA12878, and 16.5 and 11.0% on NA24385. Moreover, the overall switch errors for our method are 7.26 and 5.21 with average 3378 and 5736 SNP sites per block on NA12878 and NA24385, respectively. Here, we demonstrate that SMS reads alone can generate a high quality solution for both SNP calling and haplotype assembly.Availability and implementationSource codes and results are available at https://github.com/guofeieileen/SMRT/wiki/Software.
Generation of tumor antigen-specific murine CD8+ T cells with enhanced anti-tumor activity via highly efficient CRISPR/Cas9 genome editing
Authors: Ouchi Yasuo , Patil Ashwini , Tamura Yusuke , Nishimasu Hiroshi , Negishi Aina , Paul Sudip Kumar , Takemura Naoki , Satoh Takeshi , Kimura Yasumasa , Kurachi Makoto , Nureki Osamu , Nakai Kenta , Kiyono Hiroshi , Uematsu Satoshi 
Published: February 2018

Abstract
Immunotherapies have led to the successful development of novel therapies for cancer. However, there is increasing concern regarding the adverse effects caused by non-tumor-specific immune responses. Here, we report an effective strategy to generate high-avidity tumor-antigen-specific CTLs, using Cas9/single-guide RNA (sgRNA) ribonucleoprotein (RNP) delivery. As a proof-of-principle demonstration, we selected the gp100 melanoma-associated tumor antigen, and cloned the gp100-specific high-avidity TCR from gp100-immunized mice. To enable rapid structural dissection of the TCR, we developed a 3D protein structure modeling system for the TCR/antigen-major histocompatibility complex (pMHC) interaction. Combining these technologies, we efficiently generated gp100-specific PD-1(−) CD8+ T cells, and demonstrated that the genetically engineered CD8+ T cells have high avidity against melanoma cells both in vitro and in vivo. Our methodology offers computational prediction of the TCR response, and enables efficient generation of tumor antigen-specific CD8+ T cells that can neutralize tumor-induced immune suppression leading to a potentially powerful cancer therapeutic.
Early detection of recurrent medulloblastoma: the critical role of diffusion-weighted imaging
Authors: Aboian Mariam S , Kline Cassie N , Li Yi , Solomon David A , Felton Erin , Banerjee Anu , Braunstein Steve E , Mueller Sabine , Dillon William P , Cha Soonmee 
Published: February 2018

Abstract

Background
Imaging diagnosis of medulloblastoma recurrence relies heavily on identifying new contrast-enhancing lesions on surveillance imaging, with diffusion-weighted imaging (DWI) being used primarily for detection of complications. We propose that DWI is more sensitive in detecting distal and leptomeningeal recurrent medulloblastoma than T1-weighted postgadolinium imaging.

Methods
We identified 53 pediatric patients with medulloblastoma, 21 of whom developed definitive disease recurrence within the brain. MRI at diagnosis of recurrence and 6 months prior was evaluated for new lesions with reduced diffusion on DWI, contrast enhancement, size, and recurrence location.

Results
All recurrent medulloblastoma lesions demonstrated reduced diffusion. Apparent diffusion coefficient (ADC) measurements were statistically significantly lower (P = .00001) in recurrent lesions (mean=0.658, SD=0.072) as compared to contralateral normal region of interest (mean=0.923, SD=0.146). Sixteen patients (76.2%) with disease recurrence demonstrated contrast enhancement within the recurrent lesions. All 5 patients with nonenhancing recurrence demonstrated reduced diffusion, with a mean ADC of 0.695 ± 0.101 (normal=0.893 ± 0.100, P = .0027). While group 3 and group 4 molecular subtypes demonstrated distal recurrence more frequently, nonenhancing metastatic disease was found in all molecular subtypes.

Conclusion
Recurrent medulloblastoma lesions do not uniformly demonstrate contrast enhancement on MRI, but all demonstrate reduced diffusion. Our findings support that DWI is more sensitive than contrast enhancement for detection of medulloblastoma recurrence, particularly in cases of leptomeningeal nonenhancing disease and distal nonenhancing focal disease. As such, recurrent medulloblastoma can present as a reduced diffusion lesion in a patient with normal postgadolinium contrast MRI.
Splenic Infarction: An Under-recognized Complication of Infectious Mononucleosis?
Authors: Li Yan , George Ann , Arnaout Sami , Wang Jennifer P , Abraham George M 
Published: February 2018

Abstract
Splenic infarction is a rare complication of infectious mononucleosis. We describe 3 cases of splenic infarction attributed to infectious mononucleosis that we encountered within a 2-month period. We underscore the awareness of this potential complication of infectious mononucleosis and discuss the differential diagnosis of splenic infarction, including infectious etiologies. While symptomatic management is usually sufficient for infectious mononucleosis-associated splenic infarction, close monitoring for other complications, including splenic rupture, is mandated.