bluuebunny/crossref_metadata_2025_split huggingface dataset:
LysSYL‐Loaded pH‐Switchable Self‐Assembling Peptide Hydrogels Promote Methicillin‐Resistant Staphylococcus Aureus Elimination and Wound Healing
Authors: Liu He , Wei Xuemei , Peng Huagang , Yang Yi , Hu Zhen , Rao Yifan , Wang Zhefen , Dou Jianxiong , Huang Xiaonan , Hu Qiwen , Tan Li , Wang Yuting , Chen Juan , Liu Lu , Yang Yuhua , Wu Jianghong , Hu Xiaomei , Lu Shuguang , Shang Weilong , Rao Xiancai 
Published: November 2024

AbstractStaphylococcus aureus (S. aureus), especially methicillin‐resistant S. aureus (MRSA), causes wound infections, whose treatment remains a clinical challenge. Bacterium‐infected wounds often create acidic niches with a pH 4.5–6.5. Endolysin LysSYL, which is derived from phage SYL, shows promise as an antistaphylococcal agent. However, endolysins generally exhibit instability and possess low bioavailability in acidic microenvironments. Here, an array of self‐assembling peptides is designed, and peptide L5 is screened out based on its gel formation property and bioavailability. L5 exerted a pH‐switchable antimicrobial effect (pH 5.5) and formed biocompatible hydrogels at neutral pH (pH 7.4). The LysSYL‐loaded L5 can assemble L5@LysSYL hydrogels, increase thermal stability, and exhibit the slow‐release effect of LysSYL. Effective elimination of S. aureus is achieved by L5@LysSYL through bacterial membrane disruption and cell separation inhibition. Moreover, L5@LysSYL hydrogels exhibit great potential in promoting wound healing in a mouse wound model infected by MRSA. Furthermore, L5@LysSYL hydrogels are safe and can decrease the cytokine levels and increase the number of key factors for vessel formation, which contribute to wound healing. Overall, the self‐assembling L5@LysSYL can effectively clean MRSA and promote wound healing, which suggests its potential as a pH‐sensitive wound dressing for the management of wound infections.
Engineering Triple‐Phase Interfaces with Hierarchical Carbon Nanocages for High‐Areal‐Capacity All‐Solid‐State Li‐S Batteries
Authors: Luo Yu , Pan Siyuan , Tian JingYi , Liang Yali , Zhong Haoyue , Ma Ruqin , Gu Jiabao , Wu Yuqi , Zhang Huiyan , Lin Hongxin , Huang Weilin , Deng Yuxi , Su Yu , Gong Zhengliang , Huang Jianyu , Hu Zheng , Yang Yong 
Published: November 2024

AbstractAll‐solid‐state lithium‐sulfur batteries (ASSLSBs) have garnered widespread attention due to their advantages of high energy density and enhanced safety. However, the typical composite structure composed of solid‐state electrolyte (SE), discrete conducting carbon black, and microsized sulfur (μ‐S) with long‐range Li+/e− conducting path and huge volume changes, suffers from sluggish charge transport and severe electrochemical‐mechanical failure. In this work, a unique hierarchical carbon nanocage (hCNC) is applied as a continuous conducting network where nanosized sulfur are confined. Due to the synergistic effects of multi‐dimensional (particle, interface, and electrode) structural engineering, this new sulfur‐carbon composite cathode (S@hCNC39) can achieve uniform distribution of sulfur and carbon, and efficiently constructs triple‐phase interfaces, showing enhanced charge‐carrier transport and improved electrochemical‐mechanical stability. Remarkable cycling performance of 89% after 300 cycles at 0.2 C at 30 °C is realized in ASSLSBs assembled with S@hCNC39. Notably, ASSLSBs achieve an ultrahigh areal capacity of 9.95 mAh cm−2 with stable cycling at 60 °C with high sulfur contents of 40% and high sulfur loadings of 6 mg cm−2. These results provide critical insights into the design of rational sulfur‐carbon composites and offer a viable approach to enhance the overall performance of ASSLSBs.
Properties of Pickering Stabilized Associative Water‐In‐Water Emulsions Based on Ultra‐High Molecular Weight Polyacrylamides
Authors: Lira Rafael B. , Plucinski Alexander , Ko YouBeen , Bayliss Niamh , van Ewijk Chris , Roos Wouter H. , Schmidt Bernhard V. K. J. 
Published: November 2024

AbstractCompletely water‐based multicompartment systems have attracted a broad interest in recent years, mainly due to their versatile features such as permeability. Here, the associative formation of water‐in‐water (w/w) emulsions based on ultra‐high molecular weight poly(N,N‐dimethylacrylamide) (PDMA) and poly(4‐acryloylmorpholine) (PAM) is studied. The system is investigated using a combination of fluorescence microscopy and spectroscopy techniques. The system phase‐separates into aqueous droplets at very low polymer concentrations and exhibits intriguing physical properties. The formed emulsion droplets are extremely fluid (5–10 mPa.s), enable fast (5 µm2 s−1), nearly complete (mobile fraction ≈0.8) and unhindered diffusion within and across compartments, which is a hallmark of fluids. Furthermore, the very low interfacial tension (0.18–0.40 mN m−1) enables droplet coalescence leading to equilibrium formation of various emulsion structures. These properties show similarities to cell cytoplasm and coacervates and hence this type of w/w emulsion formed via associative non‐ionic interactions is a new direction in the field of synthetic cells and synthetic biology.
Rh(III)‐Catalyzed C−H Cyclization of 2‐Arylbenzimidazoles with CF3‐Imidoyl Sulfoxonium Ylides and Further Sc(III)‐Catalyzed Deaminative Hydroxylation
Authors: Liao Juting , Zhai Ruirui , Gao Xiaoyang , Wu Hongliang , Kong Dulin , Wang Shuojin , Chen Xun 
Published: November 2024

AbstractAn efficient Rh(III)‐catalyzed C−H bond activation/cyclization of 2‐arylbenzimidazoles with CF3‐imidoyl sulfoxonium ylides has been achieved, yielding diverse CF3− and amino‐disubstituted 5,6‐dihydrobenzoimidazo[2,1‐a]isoquinolines, which could undergo a deaminative hydroxylation to access CF3− and hydroxy‐disubstituted 5,6‐dihydrobenzoimidazo[2,1‐a]isoquinolines catalyzed by Sc(OTf)3. This developed strategy features easily available starting materials, broad substrate scope, good scalability and high efficiency. Moreover, the antitumor activities of selected products against some human cancer cell lines were also investigated, and the results indicated that several products show antiproliferative activities.
Nanoparticles Combining Host‐Directed Therapeutics and Antibiotics to Boost Bacterial Killing and Overall Survival of Zebrafish Embryos Infected with Mycobacterium Marinum
Authors: Schäfer Gabriela , Bi Dongdong , Fenaroli Federico , Thompson Andrew M. , Saris Anno , Barz Matthias 
Published: November 2024

AbstractThe bacterial pathogen, Mycobacterium tuberculosis (Mtb), remains a leading cause of global morbidity and mortality. Due to the complexity of the infection, the limited efficacy of antibiotics, and the increasing incidence of multi‐drug resistant strains, novel therapeutics are urgently needed. An interesting new approach is to combine antibiotics with treatments that boost the natural ability of host cells to kill the bacteria, so‐called host‐directed therapeutics (HDT). Until now, this approach has not been explored in the context of nanoparticle drug delivery, which may offer several advantages. Here, the HDT drug everolimus and the antibiotic ((S)‐2‐nitro‐6‐((5‐((4‐(trifluoromethoxy)phenyl)ethynyl)pyridin‐2‐yl)methoxy)‐6,7‐dihydro‐5H‐imidazo[2,1‐b][1,3]oxazine, drug D) are focused on. Both of these agents, drug D and everolimus, are encapsulated into one single micelle (PeptoMicelles), resulting in formulation C, and compared with micelles containing only drug D (formulation A) or everolimus (formulation B). They are tested in Mtb‐infected primary macrophages and THP‐1 cells, and in the zebrafish model for TB (Mm infection) using embryo survival analysis. The results show that formulation C, containing drug D and everolimus in one micelle, is significantly more efficient in protecting cells and zebrafish embryos against infection than all other formulations, indicating an improved therapeutic efficacy of HDTs and antibiotics in a single polymeric micelle.
2D α‐In2Se3 Flakes for High Frequency Tunable and Switchable Film Bulk Acoustic Wave Resonators
Authors: Sun Jiayi , Cai Weifan , Yang Yang , Zhuang Yihao , Zhang Qing 
Published: November 2024

AbstractTunable and switchable film bulk acoustic resonators (FBARs) with the capability of dynamically adjusting their resonant frequencies hold significant promise for advanced multi‐band radio frequency (RF) communication systems. However, tunable and switchable FBARs based on conventional thin ferroelectric materials face several challenges in meeting the demands of advanced RF applications. Specifically, submicron‐thick ferroelectric materials suffer from degradation in piezoelectric performance due to the strong scattering of acoustic waves caused by surface defects, as well as the inconsistency in crystal orientation. Recent advances in 2D ferroelectric materials create new opportunities for high‐performance tunable and switchable FBARs. Here, the first batch of FBAR chips based on 2D α‐In2Se3 flakes is reported. The α‐In2Se3‐based FBARs are normally under the on‐state and possess a small off‐voltage of −4 V. A tuning range of 26 MHz is achieved with a control voltage from −2 to 4 V at the resonant frequency of 8.6 GHz. To the best of the author's knowledge, this is the first batch of tunable FBARs that can function beyond the sub‐6 GHz band. This work demonstrates for the first time that 2D ferroelectric materials are very promising for high‐frequency tunable and switchable FBARs.
Evaluating Pressure‐dependent Discharge Behavior of Foil Versus In situ Plated Lithium Metal Anodes in Solid‐State Batteries
Authors: Haslam Catherine G. , Eckhardt Janis K. , Ayyaswamy Abhinand , Vishnugopi Bairav S. , Fuchs Till , Liao Daniel W. , Dasgupta Neil P. , Mukherjee Partha P. , Janek Jürgen , Sakamoto Jeff 
Published: November 2024

AbstractAnode‐free manufacturing of solid‐state batteries (SSBs) shows promise to maximize energy density by eliminating excess lithium (Li) and simplifying battery production. However, high reversibility during discharge (stripping of Li) is necessary for long‐lifetime SSBs with a limited Li reservoir. Further, the plastic flow of Li changes depending on the Li thickness, leading to possible differences in discharge performance under stack pressure. This work investigates the pressure‐dependent discharge performance of anode‐free manufactured SSBs with in situ plated Li and compares the performance to that of conventional thick Li foil cells. Distinct stripping behavior is observed at low pressures (0–1 MPa), where Li diffusivity and initial interfacial contact may control accessible capacity, compared to high pressures (3–10 MPa) where mechanical deformation of Li likely governs stripping behavior. Analysis of impedance spectra collected during stripping shows that additional stack pressure delays the formation of deep, as opposed to lateral, voids in the Li anode. These results provide insights to guide the transition from thick Li foil anodes to anode‐free manufactured SSBs.
High‐grade B‐cell lymphoma not otherwise specified, with diffuse large B‐cell lymphoma gene expression signatures: Genomic analysis and potential therapeutics
Authors: Lone Waseem , Bouska Alyssa , Herek Tyler A. , Amador Catalina , Song Joo , Xu Alexander M. , Jochum Dylan , Issa Issa Ismail , Weisenburger Dennis D. , Zhang Xuan , Bhagavathi Sharath Kumar , Heavican‐Foral Tayla B. , Sharma Sunandini , Shah Ab Rauf , Mir Abdul Rouf , Alkhinji Aisha Ahmad , El‐Gamal Dalia , Dave Bhavana J. , Hartert Keenan , Yu Jiayu , Saumyaranjan Mallick , Greiner Timothy C. , Vose Julie , McKeithan Timothy W. , Fu Kai , Green Michael , Bi Chengfeng , Merchant Akil , Chan Wing C. , Iqbal Javeed 
Published: November 2024

AbstractHigh‐grade B‐cell lymphoma not otherwise specified (HGBCL, NOS) has overlapping morphological and genetic features with diffuse large B‐cell lymphoma (DLBCL) and Burkitt lymphoma (BL), leading to uncertainty in its diagnosis and clinical management. Using functional genomic approaches, we previously characterized HGBCL and NOS, that demonstrate gene expression profiling (GEP), and genetic signatures similar to BL. Herein, we characterize distinct HGBCL, NOS, cohort (n = 55) in adults (n = 45) and in children (n = 10), and compared the GEP, genomic DNA copy number (CN), and mutational spectrum with de novo DLBCL (n = 85) and BL (n = 52). This subgroup, representing ~60% of HGBCL, NOS, lack gene‐expression signature of BL and double hit/dark zone lymphoma, but express DLBCL like signatures and are characterized by either GCB‐ or ABC‐like mRNA signatures and exhibit higher genomic complexity, similar to de novo DLBCL, and show alteration in genes regulating B‐cell activation (CD79B, MYD88, PRDM1, TBLIXR1, CARD11), epigenome (KMT2D, TET2) and cell cycle transition (TP53, ASPM). However, recurrent mutations in genes often mutated in BL (DDX3X, GNA13, CCND3), but rare in DLBCL, are also present in HGBCL‐NOS, highlighting genetic heterogeneity. Consistent with mutation spectrum, frequent genomic CN alterations in genes regulating B‐cell activation (del‐PRDM1, gain‐BCL6, ‐REL, ‐STAT3) and cell cycle regulators (del‐TP53, del‐CDKN2A, del‐RB1, gain‐CCND3) were observed. Pediatric cases showed GCB‐DLBCL‐like mRNA signatures, but also featured hallmark mutations of pediatric BL. Frequent oncogenic PIM1 mutations were present in adult HGBCL, NOS. In vitro analyses with pharmacologic or genetic inhibition of PIM1 expression triggered B‐cell activation and NF‐κB‐induced apoptosis, suggesting that PIM1 is a rational therapeutic target.
Facile Synthesis of 1,4,2‐Dioxazines Using Aromatic Chlorooximes and 2‐Chloroethanol/2‐Chlorophenols
Authors: Gladis Serrao Lavina , Rachappa Maddani Mahagundappa 
Published: November 2024

AbstractA facile and mild strategy for the synthesis of 5,6‐dihydro‐1,4,2‐dioxazines by the reaction of aromatic chlorooximes with 2‐chloroethanol in presence of NaH at room temperature in a short period of time is developed. This strategy is further extended for the synthesis of substituted 1,4,2‐dioxazines using aromatic chlorooximes and 2‐chlorophenols. Similarly, 5,6‐dihydro‐1,4,2‐dioxazepine was also synthesized using aromatic chlorooxime and 3‐bromopropanol. The developed protocol is simple, efficient and produces environmentally benign byproducts.
Elevated Cerebrospinal Fluid Ubiquitin Carboxyl‐Terminal Hydrolase Isozyme L1 in Asymptomatic C9orf72 Hexanucleotide Repeat Expansion Carriers
Authors: Dellar Elizabeth R. , Vendrell Iolanda , Amein Benazir , Lester David G. , Edmond Evan C. , Yoganathan Katie , Dharmadasa Thanuja , Sogorb‐Esteve Aitana , Fischer Roman , Talbot Kevin , Rohrer Jonathan D. , Turner Martin R. , Thompson Alexander G. 
Published: November 2024

ObjectiveTo identify biochemical changes in individuals at higher risk of developing amyotrophic lateral sclerosis (ALS) or frontotemporal dementia (FTD) via C9orf72 hexanucleotide repeat expansion (HRE) heterozygosity.MethodsCross‐sectional observational study of 48 asymptomatic C9orf72 HRE carriers, 39 asymptomatic non‐carrier controls, 19 people with sporadic ALS, 10 with C9orf72 ALS, 14 with sporadic FTD, and 10 with C9orf72 FTD. Relative abundance of 30 pre‐defined cerebrospinal fluid biomarkers of ALS and FTD were compared in asymptomatic C9orf72 HRE carriers and age‐matched non‐carrier controls. Differential abundance of these proteins was quantified using data independent acquisition mass spectrometry or electro chemiluminescent assay for neurofilament light chain. Unbiased analysis of the entire cerebrospinal fluid proteome was then carried out.ResultsUbiquitin carboxyl‐hydrolase isozyme L1 levels were higher in asymptomatic C9orf72 HRE carriers compared with age‐matched non‐carriers (log2fold change 0.20, FDR‐adjusted p‐value = 0.034), whereas neurofilament light chain levels did not significantly differ. Ubiquitin carboxyl‐hydrolase isozyme L1 levels remained elevated after matching of groups by neurofilament levels (p = 0.011), and after adjusting for age, sex, and neurofilament levels. A significant difference was also observed when restricting analysis to younger participants (<37) matched by neurofilament level (p = 0.007).InterpretationElevated cerebrospinal fluid ubiquitin carboxyl‐hydrolase isozyme L1 levels in C9orf72 HRE carriers can occur in the absence of increased neurofilament levels, potentially reflecting either compensatory or pathogenic mechanisms preceding rapid neuronal loss. This brings forward the window on changes associated with the C9orf72 HRE carrier state, with potential to inform understanding of penetrance and approaches to prevention. ANN NEUROL 2025;97:449–459
Synthesis of hydroxytyrosol analogs with enhanced antioxidant and cytostatic properties against MG‐63 human osteoblast‐like cells and their potential implications for bone health
Authors: Georgiou Eleftheria A. , Kalpaktsi Ioanna , Gioti Katerina , Choleva Maria , Fragopoulou Elizabeth , Skaltsounis Alexios‐Leandros , Tenta Roxane , Kostakis Ioannis K. 
Published: November 2024

AbstractSixteen novel hydroxytyrosol (HT) analogs with substitutions at the C‐1 position of the HT aliphatic side chain were synthesized and evaluated for their cytostatic activity against MG‐63 human osteoblast‐like cells and for their antioxidant properties. The results revealed that these analogs exhibited significantly higher inhibitory activity compared with HT, which served as the positive control. Among these, the cyclo‐substituted compounds stood out as particularly potent, demonstrating strong radical scavenging abilities and notable cytostatic effects against MG‐63 cells. These findings suggest that the cyclo‐substituted HT analogs hold considerable promise for the development of novel antioxidants with potential applications in bone physiology.
Detailed phenotyping of Tbr1‐2A‐CreER knock‐in mice demonstrates significant impacts on TBR1 protein levels and axon development
Authors: Co Marissa , O'Brien Grace K. , Wright Kevin M. , O'Roak Brian J. 
Published: November 2024

AbstractCre recombinase knock‐in mouse lines have served as invaluable genetic tools for understanding key developmental processes altered in autism. However, insertion of exogenous DNA into the genome can have unintended effects on local gene regulation or protein function that must be carefully considered. Here, we analyze a recently generated Tbr1‐2A‐CreER knock‐in mouse line, where a 2A‐CreER cassette was inserted in‐frame before the stop codon of the transcription factor gene Tbr1. Heterozygous TBR1 mutations in humans and mice are known to cause autism or autism‐like behavioral phenotypes accompanied by structural brain malformations, most frequently a reduction of the anterior commissure (AC). Thus, it is critical for modified versions of Tbr1 to exhibit true wild‐type‐like activity. We evaluated the Tbr1‐2A‐CreER allele for its potential impact on Tbr1 function and complementation to Tbr1 loss‐of‐function alleles. In mice with one copy of the Tbr1‐2A‐CreER allele, we identified reduction of TBR1 protein in early postnatal cortex along with thinning of the AC, suggesting hypersensitivity of this structure to TBR1 dosage. Comparing Tbr1‐2A‐CreER and Tbr1‐null mice to Tbr1‐null complementation crosses showed reductions of TBR1 dosage ranging from 20% to 100%. Using six combinatorial genotypes, we found that moderate to severe TBR1 reductions (≥44%) were associated with cortical layer 5 expansion, while only the complete absence of TBR1 was associated with reeler‐like “inverted” cortical layering. In total, these results strongly support the conclusion that Tbr1‐2A‐CreER is a hypomorphic allele. We advise caution when interpreting experiments using this allele, considering the sensitivity of various corticogenic processes to TBR1 dosage and the association of heterozygous TBR1 mutations with complex neurodevelopmental disorders.
Modeling Acoustic Attenuation, Sound Velocity and Wave Propagation in Lithium‐Ion Batteries via a Transfer Matrix
Authors: Feiler Simon , Gold Lukas , Hartmann Sarah , Giffin Guinevere A. 
Published: November 2024

AbstractA simple 1D transfer matrix model of a battery is introduced and parametrized using harvested individual cell components at 0 % and 100 % SoC. This model allows for the calculation of group velocity and attenuation. The results of the model show good agreement with measured values, highlighting increased attenuation and group velocity at the resonances. This emphasizes the importance of selecting a suitable interrogation frequency for ultrasound investigations in lithium‐ion batteries. The model accurately replicates the observed weakening of resonances with increasing SoC. Additionally, it provides the basis to fit US spectroscopy data in the future, enabling immediate determination of component thickness and the Young's modulus of individual components, along with aiding in the identification aging effects of the anode and cathode materials. The model can visualize wave propagation within the battery. At certain frequencies, standing waves form which could be used in high‐intensity ultrasound applications targeted at individual cell components.
Sustainable Combination of Waste with Waste: Utilization of Biomass to Recover Critical Metals from Spent Lithium ion Batteries
Authors: Jungi Hiren , Virani Asma A. , Podder Sumana , Girase Harpal , Mitra Joyee 
Published: November 2024

AbstractThe generation of e‐waste from lithium ion batteries (LIBs) is rapidly increasing due to the rising utilization of LIBs in portable electronics, and electric vehicles, with an average life span of 3–5 years. The disposal of spent LIBs is a major environmental concern due to the presence of high percentages of toxic heavy metals and corrosive electrolytes. Efficient and sustainable recycling of spent LIBs has become immensely important, both environmentally and from a circular economy perspective, as LIBs serve as secondary sources of critical metals needed for renewable energy conversion and storage systems. We provide a concise review of metal recovery from spent LIBs using waste biomass in a green and sustainable approach for resource generation, in addition to the valorization of waste biomass. Biomass is used to recover metals from spent LIBs, acting as lixivient, reductant, and as absorbent. We have also discussed a reverse strategy utilizing ‘black mass’ from spent LIBs as catalyst for biomass conversion to value‐added products. The concept of “circular economy” is highlighted in a “killing two birds with one stone” approach through the utilization of biomass for the recovery of metals from spent LIBs and vice versa.