bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Bioprinted Cardiac Patch Composed of Cardiac‐Specific Extracellular Matrix and Progenitor Cells for Heart Repair
Authors: Bejleri Donald , Streeter Benjamin W. , Nachlas Aline L. Y. , Brown Milton E. , Gaetani Roberto , Christman Karen L. , Davis Michael E. 
Published: October 2018

AbstractCongenital heart defects are present in 8 of 1000 newborns and palliative surgical therapy has increased survival. Despite improved outcomes, many children develop reduced cardiac function and heart failure requiring transplantation. Human cardiac progenitor cell (hCPC) therapy has potential to repair the pediatric myocardium through release of reparative factors, but therapy suffers from limited hCPC retention and functionality. Decellularized cardiac extracellular matrix hydrogel (cECM) improves heart function in animals, and human trials are ongoing. In the present study, a 3D‐bioprinted patch containing cECM for delivery of pediatric hCPCs is developed. Cardiac patches are printed with bioinks composed of cECM, hCPCs, and gelatin methacrylate (GelMA). GelMA‐cECM bioinks print uniformly with a homogeneous distribution of cECM and hCPCs. hCPCs maintain >75% viability and incorporation of cECM within patches results in a 30‐fold increase in cardiogenic gene expression of hCPCs compared to hCPCs grown in pure GelMA patches. Conditioned media from GelMA‐cECM patches show increased angiogenic potential (>2‐fold) over GelMA alone, as seen by improved endothelial cell tube formation. Finally, patches are retained on rat hearts and show vascularization over 14 d in vivo. This work shows the successful bioprinting and implementation of cECM‐hCPC patches for potential use in repairing damaged myocardium.
In Situ Forming Injectable Silk Fibroin Hydrogel Promotes Skin Regeneration in Full Thickness Burn Wounds
Authors: Chouhan Dimple , Lohe Tshewuzo‐u , Samudrala Pavan Kumar , Mandal Biman B. 
Published: October 2018

AbstractFull‐thickness skin wounds, associated with deep burns or chronic wounds pose a major clinical problem. Herein, the development of in situ forming hydrogel using a natural silk fibroin (SF) biomaterial for treating burn wounds is reported. Blends of SF solutions isolated from Bombyx mori and Antheraea assama show inherent self‐assembly between silk proteins and lead to irreversible gelation at body temperature. Investigation of the gelation mechanism reveals crosslinking due to formation of β‐sheet structures as examined by X‐ray diffraction and Fourier transform infrared spectroscopy. The SF hydrogel supports proliferation of primary human dermal fibroblasts and migration of keratinocytes comparable to collagen gel (Col) as examined under in vitro conditions. The SF hydrogel also provides an instructive and supportive matrix to the full‐thickness third‐degree burn wounds in vivo. A 3‐week comparative study with Col indicates that SF hydrogel not only promotes wound healing but also shows transitions from inflammation to proliferation stage as observed through the expression of TNF‐α and CD163 genes. Further, deposition and remodeling of collagen type I and III fibers suggests an enhanced overall tissue regeneration. Comparable results with Col demonstrate the SF hydrogel as an effective and inexpensive formulation toward a potential therapeutic approach for burn wound treatment.
Photo‐Powered Artificial Organelles for ATP Generation and Life‐Sustainment
Authors: Zheng Di‐Wei , Xu Lu , Li Chu‐Xin , Dong Xue , Pan Pei , Zhang Qiu‐Ling , Li Bin , Zeng Xuan , Zhang Xian‐Zheng 
Published: October 2018

AbstractAdenosine triphosphate (ATP) is the most important immediate energy source for driving intracellular biochemical reactions in nearly all life forms. Controllable generation of ATP in life is still an unrealized goal. Here, thylakoid fragments are recombined with lipid molecules to synthesize a synthetic/biological hybrid proteoliposome, named highly efficient life‐support intracellular opto‐driven system (HELIOS) for the generation of ATP. With red light irradiation, HELIOS can improve the intracellular ATP concentration to 1.38–2.45 times in various cell lines. Moreover, it is noticed that HELIOS‐mediated ATP generation can comprehensively promote cell functions such as protein synthesis and insulin secretion. At organ and individual levels, it is also proved that HELIOS can rescue a mouse heart from myocardial infarction and sustain life of fasting zebrafish Danio rerio models. The photo‐powered artificial organelle can deepen our understanding of metabolism and enable the development of optical therapy that targets intracellular energy supply.
On‐Demand Control of the Photochromic Properties of Naphthopyrans
Authors: Kuroiwa Hayato , Inagaki Yuki , Mutoh Katsuya , Abe Jiro 
Published: October 2018

AbstractPhotofunctional compounds have emerged as critically important materials for both fundamental studies and industrial applications. Control of the thermal decoloration speed to within several seconds while sustaining satisfactory photochromic colorability is an important challenge for the application of such materials to photochromic lenses and smart windows. Photochromic naphthopyran derivatives are utilized for photochromic lenses because of their high durability and easily controllable colorability. However, the residual color imparted by the long‐lived transient species upon ceasing light irradiation remains a hindrance to practical applications. In this study, a strategy is demonstrated for on‐demand control of the thermal decoloration speed of the transient colored species of naphthopyran derivatives. The increase in the ring‐size of the alkylenedioxy moiety on the naphthopyrans accelerates the thermal back‐reaction independently of the maximum‐absorption wavelength of the colored isomer, leading to the realization of yellow‐, red‐, and blue‐photochromic naphthopyrans with similar thermal fading speeds. This novel molecular design provides a strategy for the future development of advanced photoresponsive materials.
From Benzofurans to Indoles: Palladium‐Catalyzed Reductive Ring‐Opening and Closure via β‐Phenoxide Elimination
Authors: Perego Luca A. , Wagschal Simon , Grüber Raymond , Fleurat‐Lessard Paul , El Kaïm Laurent , Grimaud Laurence 
Published: October 2018

AbstractBenzofurans can undergo ring‐opening by a palladium‐catalyzed process resulting in C−O bond breaking. Benzofuran‐tethered 2‐iodoanilines give synthetically interesting 2‐(3‐indolylmethyl)phenols in an overall reductive process. Mechanistic studies suggest that this unusual reaction proceeds by carbopalladation of benzofuran giving a 3‐palladated 2,3‐dihydrobenzofuran intermediate, which then fragments by an uncommon trans‐elimination of the phenoxide group β to the metal. In this transformation, N,N‐diisopropylethylamine (DIPEA) acts as a base and as a reducing agent: it regenerates palladium(0) from palladium(II), thus allowing catalytic turnover.magnified image
Spray‐Dried Mesoporous Mixed Cu‐Ni Oxide@Graphene Nanocomposite Microspheres for High Power and Durable Li‐Ion Battery Anodes
Authors: Lefrançois Perreault Louis , Colò Francesca , Meligrana Giuseppina , Kim Kyoungsoo , Fiorilli Sonia , Bella Federico , Nair Jijeesh R. , Vitale‐Brovarone Chiara , Florek Justyna , Kleitz Freddy , Gerbaldi Claudio 
Published: October 2018

AbstractExfoliated graphene‐wrapped mesoporous Cu‐Ni oxide (CNO) nanocast composites are developed using a straightforward nanostructure engineering strategy. The synergistic effect of hierarchical mesoporous CNO nanobuilding blocks that are homogeneously wrapped by graphene nanosheets (GNSs) using a rapid spray drying technique effectively preserves the electroactive species against the volume changes resulting from the charge/discharge process. Owing to the intriguing structural/morphological features arising from the caging effect of exfoliated graphene sheets, these 3D/2D CNO@GNS nanocomposite microspheres are promising as high‐performance Li‐ion battery anode materials. They exhibit unprecedented electrochemical behavior, such as high reversible specific capacity (initial discharge capacities exceeding 1700 mAh g−1 at low 0.1 mA g−1, stable 850 and 730 mAh g−1 at 1 and 5 mA g−1 after 800 and 1300 cycles, respectively, and higher than 400 mAh g−1 at very high current density of 10 mA g−1 after more than 2000 cycles), excellent coulombic efficiency and long‐term stability (more than 3000 cycles with >55% capacity retention) at high current density that are remarkable compared to most transition metal oxides and nanocomposites prepared by conventional techniques. This simple, yet innovative, material design is inspiring to develop advanced conversion materials for Li‐ion batteries or other energy storage devices.
Reversible Photoisomerization of Monolayers of π‐Expanded Oligothiophene Macrocycles at Solid–Liquid Interfaces
Authors: Cojal González José D. , Iyoda Masahiko , Rabe Jürgen P. 
Published: October 2018

AbstractSelf‐assembled monolayers of a π‐expanded oligothiophene macrocycle undergo photoisomerization between their Z,Z and E,E diastereomers at the interface between octanoic acid solutions and highly oriented pyrolytic graphite (HOPG). The switching process proceeds in situ at the solid–liquid interface and was followed by scanning tunneling microscopy (STM). Upon illumination with light at 365 nm (546 nm), a monolayer of Z,Z‐8mer (E,E‐8mer) photoisomerizes to the E,E‐8mer (Z,Z‐8mer) form with changes in 2D hexagonal packing. These findings provide insight towards the design of photoresponsive surfaces with desirable optoelectronic and structural (host–guest) properties.
Ultrathin Nitrogen‐Doped Holey Carbon@Graphene Bifunctional Electrocatalyst for Oxygen Reduction and Evolution Reactions in Alkaline and Acidic Media
Authors: Sun Jiqing , Lowe Sean E. , Zhang Lijuan , Wang Yazhou , Pang Kanglei , Wang Yun , Zhong Yulin , Liu Porun , Zhao Kun , Tang Zhiyong , Zhao Huijun 
Published: October 2018

AbstractEfficient nonprecious‐metal oxygen reduction reaction (ORR) and oxygen evolution reaction (OER) electrocatalysts are key for the commercial viability of fuel cells, metal–air batteries, and water‐splitting systems. Thus, high‐performance ORR and OER electrocatalysts in acidic electrolytes are needed to support high‐efficiency proton exchange membrane (PEM)‐based systems. Herein, we report a new approach to design and prepare an ultrathin N‐doped holey carbon layer (HCL) on a graphene sheet that exhibits outstanding bifunctional ORR/OER activities in both alkaline and acidic media. The edge sites of HCL are utilized to achieve selective doping of highly active pyridinic‐N. The sandwiched graphene sheet provides mechanical support, stabilizes HCL structure and promotes charge transfer. The synergetic effect of the catalyst structure overcomes the drawbacks of holey graphene approaches. The resulting ORR and OER performances are equal to or better than the top‐ranked electrocatalysts.
Molecular cloning and functional analysis of small heat shock protein 19.1 gene from the Chinese oak silkworm, Antheraea pernyi
Authors: Zhang Jiawei , Li Qingqing , Sun Yu , Tian Jiwu , Hu Zaijin , Zhu Baojian , Liu Chaoliang 
Published: November 2018

AbstractSmall heat shock proteins (sHSPs) are a class of highly conserved proteins that are ubiquitously found in all types of organisms, from prokaryotes to eukaryotes. In the current study, we identified and characterized the full‐length cDNA encoding sHSP 19.1 from the oak silkworm,
Antheraea pernyi. Ap‐sHSP is 510 bp in length, and encodes a protein of 169 amino acid residues. The protein contains conserved domains found in insect sHSPs, and it belongs to the
α‐crystallin‐HSPs_p23‐like superfamily. Recombinant Ap‐sHSP was expressed in
Escherichia coli cells, and a rabbit anti‐Ap‐sHSP 19.1 antibody was generated to confirm the biological functions of
Ap‐sHSP 19.1 in
A. pernyi. Real‐time polymerase chain reaction and western blot analysis revealed that
Ap‐sHSP 19.1 expression was highest in the fat body, followed by the midgut, and the lowest expression was found in the Malpighian tubule.
Ap‐sHSP 19.1 transcript expression was significantly induced following challenge with microbial pathogens. In addition, the expression of
Ap‐sHSP 19.1 was strongly induced after heat shock. These results suggest that
Ap‐sHSP 19.1 plays a crucial role in immune responses and thermal tolerance in
A. pernyi.
Fine‐mapping of HLA class I and class II genes identified two independent novel variants associated with nasopharyngeal carcinoma susceptibility
Authors: Wang Tong‐Min , Zhou Ting , He Yong‐Qiao , Xue Wen‐Qiong , Zhang Jiang‐Bo , Zheng Xiao‐Hui , Li Xi‐Zhao , Zhang Shao‐Dan , Zeng Yi‐Xin , Jia Wei‐Hua 
Published: October 2018

AbstractBackgroundSeveral genome‐wide association studies (GWASs) have identified strong associations between genetic variants in the human leukocyte antigen (HLA) region and nasopharyngeal carcinoma (NPC). However, given the complex LD pattern in this region, the causal variants and the underlying mechanism of how genetic variants in HLA contribute to NPC development is yet to be understood.MethodsTo systematically characterize the HLA variants and their relationship to NPC susceptibility, we fine‐mapped the HLA genes based on the GWAS data of 1583 NPC cases and 972 healthy controls, using SNP2HLA with the Pan‐Asian panel as references. Stepwise conditional regression was used to identify independent association loci.ResultsInterestingly, the most significant association was the presence of Gln in HLA‐A amino acid position 62 (OR = 0.57, P = 1.41 × 10−16). The G allele of rs2894207 located between HLA‐B and HLA‐C showed protective effect of NPC development (OR = 0.52, P = 2.23 × 10−13). Additionally, amino acid Phe‐67 located in the peptide‐binding pocket of HLA‐DRB1 was identified as a novel functional variant with OR = 0.64 and P = 9.64 × 10−11. Another novel variant, Glu‐45 in HLA‐B pocket B, conferred a protective effect on NPC susceptibility (OR = 0.64, P = 5.23 × 10−8). These four variants explained 2.07% of the phenotypic variance for NPC risk.ConclusionIn summary, by fine‐mapping the HLA region in south Chinese population, we reported additional loci missed in the GWAS studies and provided a better understanding of the relationship between HLA and NPC susceptibility.
Relationship between pretreatment concentration of plasma Epstein‐Barr virus DNA and tumor burden in nasopharyngeal carcinoma: An updated interpretation
Authors: Peng Liang , Yang Yi , Guo Rui , Mao Yan‐Ping , Xu Cheng , Chen Yu‐Pei , Sun Ying , Ma Jun , Tang Ling‐Long 
Published: October 2018

AbstractBackgroundPretreatment plasma Epstein‐Barr virus (EBV) DNA is an important tumor marker and prognostic factor in nasopharyngeal carcinoma (NPC). This study aimed to clarify the relationship between plasma EBV DNA level and tumor burden.Materials and MethodsPretreatment tumor burden was measured by radiologically delineated volumes, including nasopharynx tumor volume (GTVnx) and malignant nodes volume (GTVnd); pretreatment level of plasma EBV DNA was quantified by quantitative polymerase chain reaction. The relationship between natural logarithm of EBV DNA (ln‐DNA) and square root of tumor volume (sq‐GTV) was analyzed by Pearson correlation coefficient and partial correlation coefficient. Correlations in subgroups of tumor and nodal stages were also analyzed. A linear regression model was constructed to evaluate the contribution of tumor volumes to plasma EBV DNA. The prognostic effects of EBV DNA independent of tumor burden were evaluated.ResultsTwo thousand two hundred and forty nine nonmetastatic NPC patients with detectable plasma EBV DNA were included in correlation analyses. Ln‐DNA showed significant correlation with sq‐GTVnx (r = 0.171) and sq‐GTVnd (r = 0.339) separately. Together, sq‐GTVnx and sq‐GTVnd could only explain 12.9% of the ln‐DNA. Tumor and nodal stages of disease could clearly influence the strength of relationship in subgroup analysis. After excluding confounding volume information, EBV DNA still can predict death and distant metastasis, but not locoregional relapse.ConclusionThis study suggests that plasma EBV DNA is not only an index of tumor burden, but may also reflect other tumor features, such as accessibility to circulation, angiogenesis, tumor cell kinetics, metabolic activity, and metastatic potential, among others.
Binding and Enhanced Binding between Key Immunity Proteins TRAF6 and TIFA
Authors: Huang Wei‐Cheng , Liao Jiahn‐Haur , Hsiao Tzu‐Chun , Wei Tong‐You Wade , Maestre‐Reyna Manuel , Bessho Yoshitaka , Tsai Ming‐Daw 
Published: October 2018

AbstractHuman tumor necrosis factor receptor associated factor (TRAF)‐interacting protein, with a forkhead‐associated domain (TIFA), is a key regulator of NF‐κB activation. It also plays a key role in the activation of innate immunity in response to bacterial infection, through heptose 1,7‐bisphosphate (HBP); a metabolite of lipopolysaccharide (LPS). However, the mechanism of TIFA function is largely unexplored, except for the suggestion of interaction with TRAF6. Herein, we provide evidence for direct binding, albeit weak, between TIFA and the TRAF domain of TRAF6, and it is shown that the binding is enhanced for a rationally designed double mutant, TIFA S174Q/M179D. Enhanced binding was also demonstrated for endogenous full‐length TRAF6. Furthermore, the structures of the TRAF domain complexes with the consensus TRAF‐binding peptides from the C terminus of wild‐type and S174Q/M179D mutant TIFA, showing salt‐bridge formation between residues 177–181 of TIFA and the binding pocket residues of the TRAF domain, were solved. Taken together, the results provide direct evidence and a structural basis for the TIFA–TRAF6 interaction, and show how this important biological function can be modulated.
Percutaneous coronary intervention with drug‐coated balloon‐only strategy in stable coronary artery disease and in acute coronary syndromes: An all‐comers registry study
Authors: Uskela Sanna , Kärkkäinen Jussi M. , Eränen Jaakko , Siljander Antti , Mäntylä Pirjo , Mustonen Juha , Rissanen Tuomas T. 
Published: October 2018

AbstractObjectivesThe aim of this single center all‐comers retrospective registry study was to assess the efficacy and safety of percutaneous coronary intervention (PCI) using drug‐coated balloon (DCB) in de novo lesions including large proximal coronary arteries.MethodsA total of 487 PCIs were performed using paclitaxel‐coated DCB in 562 de novo lesions with the possibility for bailout stenting in a patient population presenting with stable coronary artery disease (CAD) or acute coronary syndrome (ACS). Half of the patients had at least one risk factor for bleeding. All of the treated lesions were de novo and 60% of DCBs used were ≥ 3.0 mm in diameter. The median follow‐up time was 18 months for MACE and 60 months for survival.ResultsThe total mortality after DBC only strategy was 2.3 and 9.3% at 12 months in stable CAD and ACS, respectively. The 12‐month MACE rate was 7.1 and 12% in stable CAD and ACS. The rate of ischemia‐driven target lesion revascularization was only 1.4% in stable CAD and 2.8% after ACS at 12 months. Median duration of DAPT was one month. The 12 month rate of significant bleeding (Bleeding Academic Research Consortium types 2–5) was 5.9%. Acute vessel closure occurred only in one case (0.2%) after DCB treatment. Bailout stenting was used in 12% of lesions.ConclusionsPCI using DCB‐only strategy with the possibility for provisional stenting is a safe and efficient in de novo coronary artery lesions in both stable CAD and ACS. This strategy may be useful especially in patients with high bleeding risk.
Probing the Location and Speciation of Elements in Zeolites with Correlated Atom Probe Tomography and Scanning Transmission X‐Ray Microscopy
Authors: Schmidt Joel E. , Ye Xinwei , van Ravenhorst Ilse K. , Oord Ramon , Shapiro David A. , Yu Young‐Sang , Bare Simon R. , Meirer Florian , Poplawsky Jonathan D. , Weckhuysen Bert M. 
Published: October 2018

AbstractCharacterizing materials at nanoscale resolution to provide new insights into structure property performance relationships continues to be a challenging research target due to the inherently low signal from small sample volumes, and is even more difficult for nonconductive materials, such as zeolites. Herein, we present the characterization of a single Cu‐exchanged zeolite crystal, namely Cu‐SSZ‐13, used for NOX reduction in automotive emissions, that was subject to a simulated 135,000‐mile aging. By correlating Atom Probe Tomography (APT), a single atom microscopy method, and Scanning Transmission X‐ray Microscopy (STXM), which produces high spatial resolution X‐ray Absorption Near Edge Spectroscopy (XANES) maps, we show that a spatially non‐uniform proportion of the Al was removed from the zeolite framework. The techniques reveal that this degradation is heterogeneous at length scales from micrometers to tens of nanometers, providing complementary insight into the long‐term deactivation of this catalyst system.
Smart Wetting Control on Shape Memory Polymer Surfaces
Authors: Zhang Dongjie , Cheng Zhongjun , Liu Yuyan 
Published: October 2018

AbstractControl of surface wettability is very important, and can be realized by controlling surface chemistry or microstructures. Compared with surface chemistry, smart control of surface microstructure is more difficult. Recently, shape memory polymers (SMPs) have advanced to allow control of the surface microstructure and wettability, and thus, demonstrate excellent controllability and many novel functions. In this Minireview, recent achievements in wetting control on SMP surfaces with general hydrophobic, superhydrophobic, superomniphobic and superslippery properties are presented. Particular attention is paid to superhydrophobic surfaces, which display many novel functions, such as switchable isotropic/anisotropic wetting and reprogrammable gradient wetting. Furthermore, a new strategy that combines responsive molecules with the SMP microstructure is also described; this can be used to realize precise wetting control based on coordinated regulation of both surface microstructure and chemistry.
Intertwined Titanium Carbide MXene within a 3 D Tangled Polypyrrole Nanowires Matrix for Enhanced Supercapacitor Performances
Authors: Le Thi Anh , Tran Ngoc Quang , Hong Yeseul , Lee Hyoyoung 
Published: October 2018

AbstractThe exploration of the rational design and synthesis of unique and robust architectured electrodes for the high capacitance, rate capability, and stability of supercapacitors is crucial to the future of energy storage technology. Herein, an in situ synthesis of multilayered titanium carbide MXene tightly caging within a 3 D conducting tangled polypyrrole (PPy) nanowire (NW) network is proposed as an effective strategy to prevent the aggregation of MXene, profoundly enhancing the electrochemical performance of the supercapacitor. Owing to the beneficial effects of an ideal 3 D interconnected porous structure and high electrical conductivity, the obtained electrode exhibits fast charge and ion transport kinetics as well as full usage of active material. As expected, the 3 D Ti3C2Tx@PPY NW exhibits a specific capacitance five times higher than that of pristine MXene (610 F g−1), a good rate capability up to a current density of 25 A g−1, and excellent stability with 100 % retention after 14 000 cycles at 4 A g−1, outperforming the known state‐of‐the‐art MXene‐based supercapacitor. Our work provides a facile method for enhancing the performance of MXene‐based energy storage devices.