bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of EST‐SSR markers for Pluchea indica (Asteraceae) and cross‐amplification in related species
Authors: Yang Yi , Huang Sixin , Wang Yanling , Gui Lingjian , Liu Yiran , Huang Xiaomei , Chen Guoqingzi , Tan Fengxiao , Wang Jianwu 
Published: August 2018

Premise of the StudyExpressed sequence tag–simple sequence repeat (EST‐SSR) markers were developed for Pluchea indica, a traditional medicinal species widespread along the tropical coastlines of Asia and northern Australia.Methods and ResultsBased on transcriptome data for P. indica, a total of 40 primer pairs were initially designed and tested, of which 17 were successfully amplified and showed clear polymorphism. For these SSR loci, one to nine alleles per locus were identified. The levels of observed and expected heterozygosity ranged from 0 to 0.9 and 0 to 0.831, respectively. Furthermore, 16, 17, and 12 loci were successfully amplified in three congeneric species, P. eupatorioides, P. pteropoda, and P. sagittalis, respectively.ConclusionsThe SSR markers described here may be useful for further investigation of the population genetics of P. indica and related species.
Association of Changes in Effusion‐Synovitis With Progression of Cartilage Damage Over Eighteen Months in Patients With Osteoarthritis and Meniscal Tear
Authors: MacFarlane Lindsey A. , Yang Heidi , Collins Jamie E. , Jarraya Mohamed , Guermazi Ali , Mandl Lisa A. , Martin Scott D. , Wright John , Losina Elena , Katz Jeffrey N. ,   the MeTeOR Investigator Group
Published: August 2018

ObjectiveSynovitis is a feature of knee osteoarthritis (OA) and meniscal tear and has been associated with articular cartilage damage. This study was undertaken to examine the associations of baseline effusion‐synovitis and changes in effusion‐synovitis with changes in cartilage damage in a cohort with OA and meniscal tear.MethodsWe analyzed data from the Meniscal Tear in Osteoarthritis Research (MeTeOR) trial of surgery versus physical therapy for treatment of meniscal tear. We performed semiquantitative grading of effusion‐synovitis and cartilage damage on magnetic resonance imaging, and dichotomized effusion‐synovitis as none/small (minimal) and medium/large (extensive). We assessed the association of baseline effusion‐synovitis and changes in effusion‐synovitis with changes in cartilage damage size and depth over 18 months, using Poisson regression models. Analyses were adjusted for patient demographic characteristics, treatment, and baseline cartilage damage.ResultsWe analyzed 221 participants. Over 18 months, effusion‐synovitis was persistently minimal in 45.3% and persistently extensive in 21.3% of the patients. The remaining 33.5% of the patients had minimal synovitis on one occasion and extensive synovitis on the other. In adjusted analyses, patients with extensive effusion‐synovitis at baseline had a relative risk (RR) of progression of cartilage damage depth of 1.7 (95% confidence interval [95% CI] 1.0–2.7). Compared to those with persistently minimal effusion‐synovitis, those with persistently extensive effusion‐synovitis had a significantly increased risk of progression of cartilage damage depth (RR 2.0 [95% CI 1.1–3.4]).ConclusionOur findings indicate that the presence of extensive effusion‐synovitis is associated with subsequent progression of cartilage damage over 18 months. The persistence of extensive effusion‐synovitis over time is associated with the greatest risk of concurrent cartilage damage progression.
Smartly Designed Hierarchical MnO2@Fe3O4/CNT Hybrid Films as Binder‐free Anodes for Superior Lithium Storage
Authors: Yan Du‐Juan , Zhu Xiao‐Dong , Gao Xiao‐Tian , Gu Liang‐Liang , Feng Yu‐Jie , Sun Ke‐Ning 
Published: August 2018

AbstractNowadays, the development of advanced anode materials is highly desirable for the increasing demand for high‐performance lithium‐ion batteries (LIBs). Nanostructured MnO2 has received utmost attention due to high theoretical capacity (1230 mA h g−1), abundant resources, environmental benignity, and shortened electron and ion diffusion paths. Unfortunately, poor electronic conductivity and strong aggregation inclination of MnO2 nanostructures result in disappointing electrochemical performances, which restrict their practical application as sole institute. Here, we propose smartly designed MnO2@Fe3O4/CNT hybrid films, in which MnO2 nanosheets, Fe3O4 nanoparticles and CNTs are hierarchically assembled in a unique stage of nanosheets–nanoparticles–nanotubes. The resulting MnO2@Fe3O4/CNT hybrid films can be directly used as anodes without any polymer binders, and exhibit significant synergistic interactions among three components, achieving excellent reversible capacity and rate performance.
Rational Synthesis of Chabazite (CHA) Zeolites with Controlled Si/Al Ratio and Their CO2/CH4/N2 Adsorptive Separation Performances
Authors: Guo Ya , Sun Tianjun , Gu Yiming , Liu Xiaowei , Ke Quanli , Wei Xiaoli , Wang Shudong 
Published: August 2018

AbstractSeparation of CO2 from CH4 and N2 is of great significance from the perspectives of energy production and environment protection. In this work, we report the rational synthesis of chabazite (CHA) zeolites with controlled Si/Al ratio by using N,N,N‐trimethyl‐1‐adamantammonium hydroxide (TMAdaOH) as an organic structure‐directing agent, wherein the dependence of TMAdaOH consumption on the initial Si/Al ratio was investigated systematically. More TMAdaOH is required to direct the crystallization of CHA with higher Si/Al ratio. Once the product Si/Al ratio is larger than 24, the amount of TMAdaOH consumption remains nearly constant. CHA zeolites with different Si/Al ratios and charge‐compensating cations were then applied for the separation of CO2/CH4/N2 mixtures. The equilibrium selectivities predicted by ideal adsorbed solution theory (IAST) and ideal selectivities calculated from the ratio of Henry's constants for both CO2/CH4 and CO2/N2 decrease with the zeolite Si/Al ratio increasing, whereas the percentage regenerability of CO2 presents the opposite trend. Therefore, there is a trade‐off between adsorption selectivity and regenerability for the adsorbents. There is a weaker interaction between CO2 molecules and the H‐type zeolites than that on the Na‐type ones, thus a higher regenerability can be achieved. This study indicates that it is possible to design CHA zeolites with different physicochemical properties to meet various adsorptive separation requirements.
The Reactivity of Mercapto Groups against Boron Hydrides in Pincer Ligated Nickel Mercapto Complexes
Authors: Zhang Jie , Liu Ting , Wei Changgeng , Chang Jiarui , Ma Qiang‐Qiang , Li Shujun , Ma Nana , Chen Xuenian 
Published: August 2018

AbstractSeveral pincer ligated nickel mercapto complexes, [2,6‐(R2PCH2)2C6H3]NiSH (R=tBu, 1 a; iPr, 1 b), [2,6‐(R2PO)2C6H3]NiSH (R=tBu, 2 a; iPr, 2 b) and [4‐MeOCO‐2,6‐(tBu2PO)2C6H2]NiSH (3 a), were synthesized and fully characterized. The reactivity of the mercapto groups against boron hydrides and organic bases was investigated. It was found that the mercapto groups are difficult to be deprotonated by boron hydrides or organic bases. The treatment of complex 2 a or 2 b with an excess amount of catecholborane (HBcat) afforded the corresponding pincer ligated nickel borohydride complexes and the HBcat degradation product. The treatment of complex 1 a, 2 a or 2 b with an excess amount of BH3⋅THF produced the corresponding nickel borohydride species and the S‐bridged triborane species THF⋅BH2‐μ2‐S(B2H5) (5). No reactions between these complexes and organic bases were observed. DFT calculations were carried out to understand this reactivity and get mechanistic insights into the reactions.
BODIPY‐based Carbonaceous Materials for High Performance Electrical Capacitive Energy Storage
Authors: Wang De‐Gao , Wang Huan , Song Min , Yu Guipeng , Kuang Gui‐Chao 
Published: August 2018

AbstractIncorporation of heteroatoms into carbon materials is crucial to tailor carbon materials’ donor‐acceptor properties and consequently tune their chemical and electrical performance. However, doping carbon materials with multi‐heteroatoms (three or more, especially the light elements) has been rarely payed attention until now. In this work, we develop a porous organic polymer containing boron, nitrogen, fluorine elements and realize its corresponding carbonaceous materials by subsequent carbonation or KOH activation process. Electrochemical measurements results reveal that KOH activation sample (BPOP‐700A) is endowed with excellent specific capacitance of 408 F g−1 in three‐electrode system and an energy density over 19.2 Wh kg−1 in two‐electrode system at aqueous electrolyte, which can be attributed to multi light elements (boron, nitrogen and trace fluorine) doping, favorable morphology and rational pore size distribution architecture. In addition, BPOP‐700A displays well rate ability and outstanding cycle performance (10 A g−1, 10 000 cycles, 95 % retention). Our work might give some hints in designing multi light element doping carbon materials in supercapacitor applications.
Cobalt and Nitrogen Codoped Carbon Nanosheets Templated from NaCl as Efficient Oxygen Reduction Electrocatalysts
Authors: Zhou Qing‐Yan , Zhao Lei , Sui Xu‐Lei , Gong Xiao‐Fei , Li Jia‐Zhan , Li Xi‐Fei , Wang Zhen‐Bo 
Published: August 2018

AbstractThe oxygen reduction reaction (ORR) in a cathode is an essential component of many electrochemical energy storage and conversion systems. Two‐dimensional materials are beneficial for electron conduction and mass transport with high density, showing prominent electrochemical catalytic performance towards the ORR. Herein, a simple NaCl‐assisted method to synthesize cobalt–nitrogen‐doped carbon materials (CoNC), which present prominent performance towards the ORR in alkaline media, is described. The utilization of the NaCl template endows the product with a large specific surface area of 556.4 m2 g−1, as well as good dispersion of cobalt nanoparticles. CoNC‐800@NaCl (800 indicates the calcination temperature in °C) displays an excellent onset potential of 0.94 V (vs. a reversible hydrogen electrode), which is close to that of commercial Pt/C. Additionally, CoNC‐800@NaCl also exhibits better long‐term durability and methanol tolerance than that of Pt/C. The high‐performance CoNC‐800@NaCl catalyst provides a hopeful alternative to noble‐metal catalysts for the ORR in practical applications.
Comparison of aqueous soluble proteins profile of Mycobacterium tuberculosis H37Rv and H37Ra and a Malaysian clinical isolate
Authors: Kai‐Cheen Ang , Lay‐Harn Gam 
Published: August 2018

AbstractDifferentially expressed aqueous soluble proteins between Mycobacterium tuberculosis H37Ra and H37Rv were identified. The protein extracts were separated by two‐dimensional gel electrophoresis followed by tandem mass spectrometric analysis. Twelve proteins were detected to be differentially expressed significantly between virulent strain H37Rv and attenuated strain H37Ra. The differentially expression of these proteins was validated by a recently isolated clinical virulent strains of M. tuberculosis, TB138. Out of the 12 proteins identified, which consisted of ten upregulated and two downregulated proteins, nine were belonged to intermediate metabolism and respiration protein group, two were in lipid metabolism, and one protein was involved in information pathways and virulence. Among these proteins, two of the upregulated proteins, namely, mmsA and pntAa, showed a consistent expression pattern in both virulent mycobacterium strains. These proteins can serve as potential biomarkers for the intervention treatment of TB.
Advances in microalgal lipid extraction for biofuel production: a review
Authors: Harris Jesse , Viner Kelsey , Champagne Pascale , Jessop Philip G. 
Published: August 2018

AbstractAlgal biomass is an attractive feedstock for sustainable biofuel production because of its high growth rate and the fact that it does not compete with food crops. This review examines progress made in the processing and extraction of microalgal lipids as feedstocks for algae‐derived biofuels. The discussion focuses on lipid extraction processes but also mentions drying, cell disruption, and transesterification processes because of their potential effect on the extraction process, and because of the possibility of performing them simultaneously with extraction. Some of the common themes discussed include the benefits of utilizing wet microalgal biomass, combining process steps (process intensification), and the importance of considering the entire life cycle when assessing the ‘greenness’ of a technology. Lipid extraction technologies will need to be improved for microalgal biofuels to compete effectively with fossil fuels, particularly through the development of energy‐efficient extraction methods and the adaptation of these methods for large‐scale production. © 2018 Society of Chemical Industry and John Wiley & Sons, Ltd
Lignocellulosic biomass for chemicals and energy: an integrated assessment of future EU market sizes, feedstock availability impacts, synergy and competition effects, and path dependencies
Authors: Londo Marc , van Stralen Joost , Uslu Ayla , Mozaffarian Hamid , Kraan Carolien 
Published: August 2018

AbstractLignocellulosic biomass is expected to play an important role in decarbonizing our economy. In this modeling study, we assessed the future role of biobased chemicals and energy from this resource in the EU up to 2030. The study's general outcomes indicate that biobased heat remains the largest biobased sector over this period, and biobased chemicals remain the smallest. A significant share of EU‐domestic lignocellulosic biomass potential remains unutilized, even when feedstock availability is restricted. The technology mix for biobased heat, power, biofuels, and chemicals remains relatively stable in all cases, with a strong role for biobased combined heat and power (CHP). Several specific ‘what if’ analyses were done. These show that both restriction of feedstock potential and active mobilization of available potential affect overall costs of biobased options, illustrating the relevance of specific policies in bringing biomass potentials to the market. In feedstock‐restricted scenarios, specific attention to advanced lignocellulose‐based fuel technologies is essential to meet biofuel ambitions. Another analysis indicates that both competition and synergy effects occur between energy and chemical applications of biomass. This illustrates that biorefining and co‐production of biobased chemicals and energy in integrated biorefineries is a better and more robust business than separated production. Finally, an analysis of the dynamics between lignocellulosic and crop‐based biofuels reveals that the current capacity of the latter creates a need for a specific subtarget for lignocellulose‐based biofuels before they can enter the market, and that crop‐based biofuels may be gradually phased out, but with significant additional costs. © 2018 Society of Chemical Industry and John Wiley & Sons, Ltd
An aptasensor for rapid and sensitive detection of estrogen receptor alpha in human breast cancer
Authors: Ahirwar Rajesh , Dalal Anita , Sharma Jai G. , Yadav Birendra K. , Nahar Pradip , Kumar Ashok , Kumar Saroj 
Published: August 2018

AbstractThe analysis of estrogen receptor (ER) expression in breast carcinomas plays a crucial role in determining the endocrine responsiveness of tumors for systemic adjuvant therapy. Conventionally, the ER levels in breast carcinomas had been detected using the dextran‐coated charcoal assay and radioimmunoassay, which are now substituted with safer and economic antibody‐based assays such as immunohistochemistry (IHC) and enzyme‐linked immunosorbent assay (ELISA). Despite a gold (Au) standard method, the IHC has been criticized for factors such as tissue fixation, antibody selection, and threshold staining for result interpretation that could falsify test accuracy and reproducibility. The quest for alternative methods of ER quantification in tissue samples paved the way for aptamer‐based diagnostics. Previously, we have isolated a DNA aptamer against human ER alpha (ERα) using an in vitro evolution system. In this study, we developed an electrochemical sensor using the 76‐nucleotide DNA ERα‐ aptamer for rapid, precise, and cost‐effective detection of ERα expression in human breast cancer patients. The aptasensor was constructed by covalently immobilizing the thiolated ERα‐ aptamer onto a screen‐printed Au electrode. Construction of aptasensors was confirmed through atomic force microscopy and differential pulse voltammetry measurements. A detection limit of 0.001 ng/ml was calculated for full‐length ERα (66.2 kDa) in a detection time of 10 min. Analysis of the cancerous breast tissue samples using the ELISA and aptasensor methods enabled distinctive classification of samples into the categories of ER −ve, weak ER +ve, and strong ER +ve samples. The current change of this aptasensor lies within 5% after a storage of 60 days at 4°C. Further studies on a reasonably large sample size are required to realize the clinical potential of the sensor.
Development and validation of stability indicating reversed‐phase liquid chromatographic method for simultaneous quantification of methotrexate and teriflunomide in nanoparticles and marketed formulation
Authors: Pandey Shweta , Mahtab Asiya , Singh Archu , Ahmad Farhan Jalees , Aqil Mohd. , Talegaonkar Sushama 
Published: August 2018

AbstractMethotrexate (MTX) and teriflunomide (TEF) are the two most effective disease‐modifying antirheumatic drugs used as combination therapy for rheumatoid arthritis and no robust high‐performance liquid chromatography (HPLC) method is available for their simultaneous estimation to date. Therefore, we have developed and validated an isocratic reversed‐phase HPLC method for simultaneous analysis of MTX and TEF spiked in the form of active pharmaceutical ingredients, tablets and nanoformulations. The best separation was achieved on a BDS, C18, 4.6 × 250 mm, 5 μm analytical column (Thermo Hypersil) with methanol–ethylammonium formate–potassium dihydrogen phosphate buffer (55 mm, pH 3.5; 65:5:30, v/v) as mobile phase at a flow rate of 0.8 mL/min. All the samples were subjected to force degradation studies. Responses of MTX and TEF were found to be a linear function of concentration over the range 1–50 μg/mL (r2 = 0.9976 and 0.9982). The limits of detection and limit of quantification were 7.74 and 25.82 ng/mL and 10.74 and 35.80 ng/mL, respectively. Degradation products produced under the stress studies did not interfere with the detection of MTX and TEF and therefore the developed method can be regarded as stability indicating.
Focal radiation necrosis of the brain in patients with melanoma brain metastases treated with pembrolizumab
Authors: Du Four Stephanie , Janssen Yanina , Michotte Alex , Van Binst Anne‐Marie , Van den Begin Robbe , Duerinck Johnny , Neyns Bart 
Published: August 2018

AbstractIntroductionUp to 60% of patients with metastatic melanoma develop melanoma brain metastasis (MBM) during the course of their disease. Surgery, radiosurgery (SRS), stereotactic radiotherapy (SRT), and whole‐brain radiation therapy (WBRT) or combinations of these are commonly used local treatment modalities. Inhibitory monoclonal antibodies against the CTLA‐4 and PD‐1 immune checkpoint receptors significantly improved the survival of metastatic melanoma patients, including patients with MBM. This prolonged survival, and potentially also the immunostimulatory mechanisms, may expose patients to a higher risk for long‐term complications such as focal postradiation necrosis of the brain (RNB).MethodsWe analyzed the incidence of pseudotumoral RNB in a single institution cohort of 142 melanoma patients that were prospectively followed after starting treatment with pembrolizumab in an expanded access program.ResultsOf the 142 patients, 43 (30.7%) patients had MBM at initiation pembrolizumab. Of these, 31 (72.1%) were treated with SRS, 8 (18.6%) with WBRT while 4 (9.3%) had no prior local therapy. Of patients treated with RT, 28 (71.1%) received RT before the initiation of pembrolizumab. 5 (12.8%) patients developed a new symptomatic pseudotumoral lesion at a median time of 11.15 months (range 8‐46) after the RT. In all patients, the diagnosis of RNB was radiologically confirmed. The RNB was treated with corticosteroids in two patients, bevacizumab in two patients, and surgery in three symptomatic patients. The diagnosis was histologically confirmed in the patients treated with surgery.ConclusionMelanoma patients with MBM treated with radiosurgery and showing a beneficial response to pembrolizumab are at risk for late RNB. In case of suspected isolated progression at the site of a previously irradiated MBM, the diagnosis of RNB should be considered.
Proteome‐Wide Identification of On‐ and Off‐Targets of Bcl‐2 Inhibitors in Native Biological Systems by Using Affinity‐Based Probes (AfBPs)
Authors: Wang Ziqian , Guo Zongwei , Song Ting , Zhang Xiaodong , He Nianzhe , Liu Peng , Wang Peiran , Zhang Zhichao 
Published: August 2018

AbstractSelective inhibition of proteins of the Bcl‐2 family by small‐molecule inhibitors is a promising new approach in drug discovery. However, information about how these molecules interact with their cellular targets (on‐ and off‐) is highly limited. We have designed and synthesized photoreactive and “clickable” affinity‐based probes (AfBPs)—Nap‐2 and Nap‐5—by introducing photo‐crosslinkers onto Nap‐1, a fluorescent derivative of small‐molecule Bcl‐2 inhibitor S1‐6. The resulting trifunctional probes Nap‐2 and Nap‐5 can enrich, visualize, and enable the identification of cellular on‐ and off‐targets of Bcl‐2 inhibitors both in vitro and in situ. Tubulin was validated as an off‐target of Bcl‐2 inhibitors (Nap‐1 and S1‐6) by large‐scale cell‐based proteome profiling and pull‐down/western blotting (PD/WB) with Nap‐2 and Nap‐5. It was preliminarily illustrated to be a BH3‐containing protein because some well‐known Bcl‐2 inhibitors can block the labeling of tubulin by Nap‐2.
Recurrent Sweet's syndrome in a patient with multiple myeloma
Authors: Gurnari Carmelo , Franceschini Luca , Anemona Lucia , Passarelli Francesca , Vaccarini Sara , Pupo Livio , Provenzano Ida , Nasso Daniela , Rizzo Manuela , Cantonetti Maria 
Published: August 2018

Key Clinical MessageWe report on a case of Sweet's syndrome associated with multiple myeloma, as harbinger for disease relapse.
Unveiling Hidden Catalysts for the Oxidative Coupling of Methane based on Combining Machine Learning with Literature Data
Authors: Takahashi Keisuke , Miyazato Itsuki , Nishimura Shun , Ohyama Junya 
Published: August 2018

AbstractThe front cover artwork for Issue 15/2018 is provided by National Institute for Materials Science; ICAT, Hokkaido University, Japan Advanced Institute of Science and Technology, and Nagoya University (all in Japan) as part of CREST: Catalysts Informatics Project. The image shows machine learning as a tool for catalyst design based on experimental catalysis data. See the Full Paper itself at https://doi.org/10.1002/cctc.201800310.