bluuebunny/crossref_metadata_2025_split huggingface dataset:
The K2P‐channel TASK1 affects Oligodendroglial differentiation but not myelin restoration
Authors: Albrecht Stefanie , Korr Sabrina , Nowack Luise , Narayanan Venu , Starost Laura , Stortz Franziska , Araúzo‐Bravo Marcos J. , Meuth Sven G. , Kuhlmann Tanja , Hundehege Petra 
Published: January 2019

AbstractIn multiple sclerosis, demyelination occurs as a consequence of chronic autoimmunity in the central nervous system causing progressive neurological impairment in patients. After a demyelinating event, new myelin sheaths are formed by adult oligodendroglial progenitor cells; a process called remyelination. However, remyelination often fails in multiple sclerosis due to insufficient recruitment and differentiation of oligodendroglial precursor cells. A pivotal role for the two‐pore‐domain potassium (K2P) channel, TASK1, has already been proven for an animal model of multiple sclerosis. However, the mechanisms underlying the TASK1‐mediated effects are still elusive. Here, we tested the role of TASK1 channels in oligodendroglial differentiation and remyelination after cuprizone‐induced demyelination in male mice. We found TASK1 channels to be functionally expressed on primary murine and human, pluripotent stem cell‐derived oligodendrocytes. Lack of TASK1 channels resulted in an increase of mature oligodendrocytes in vitro as well as a higher number of mature oligodendrocytes and accelerated developmental myelination in vivo. Mechanistically, Task1‐deficient cells revealed a higher amount of phosphorylated WNK1, a kinase known to be involved in the downstream signaling of the myelination regulator LINGO‐1. Furthermore, we analyzed the effect of genetic TASK1 ablation or pharmacological TASK1 inhibition on disease‐related remyelination. Neither channel inhibition nor lack of TASK1 channels promoted remyelination after pathological demyelination. In summary, we conclude that functional TASK1 channels participate in the modulation of differentiating oligodendroglial cells in a previously unknown manner. However, while being involved in developmental myelination our data suggest that TASK1 channels have no major effect on remyelination.
Histopathological features of juvenile‐onset laryngeal papillomatosis related to severity
Authors: El Achkar Vivian Narana Ribeiro , Duarte Andressa , Carlos Román , León Jorge Esquiche , Ribeiro‐Silva Alfredo , Pignatari Shirley Shizue Nagata , Kaminagakura Estela 
Published: January 2019

AbstractBackgroundLaryngeal papillomatosis (LP) is a disease that presents in both juvenile (JLP) and adult patients (ALP). This study correlated papillomatosis characteristics with the Derkay score.MethodsRetrospective data and biopsies of 36 patients with JLP and 56 with ALP were collected and separated into groups according to their scores.ResultsThe mean of the Derkay score, in the JLP group was 10.97 and in Group ALP was 8.26. The JLP group presented a more aggressive result than in the adult group (P = .02). In the JLP group, the respiratory difficulty (P = .01) and tracheostomy were correlated to a higher Derkay score (P < .05). Microscopically, the JLP samples presented a higher incidence of atypical mitosis and mitosis above the basal cells layer of the epithelium (P < .05) and these characteristics were correlated with a higher Derkay index (P = .03).ConclusionFindings suggest that ALP and JLP can present different clinical courses and histopathological features. There was a higher degree of LP severity in JLP.
Therapeutic thrombocytapheresis for extreme thrombocytosis after chemotherapy in essential thrombocytosis
Authors: Almeida‐Dias Raquel , Garrote Marta , Cid Joan , Mustieles Maria‐Jesús , Alba Cristina , Lozano Miquel 
Published: January 2019

AbstractEssential thrombocytosis (ET) is a chronic myeloproliferative neoplasm characterized by the presence of thrombocytosis and it can be complicated by thrombotic and/or hemorrhagic events. Treatment options include low‐dose aspirin and cytoreductive agents such as hydroxyurea. In cases of extreme thrombocytosis, therapeutic thrombocytapheresis can be a useful procedure. We present a case of a 61‐year‐old‐man previously diagnosed with CALR‐mutated ET, who develop acute myeloid leukemia. When recovering after induction chemotherapy, he developed an extreme thrombocytosis up to 2337 × 109/L regardless hydroxyurea was started. Two therapeutic trombocytapheresis were performed and anagrelide was added to cytoreductive regimen. Platelet count stabilized around 570 × 109/L. Both procedures were performed with the Spectra Optia Apheresis System version 11.3 (Terumo BCT) and we decided to use a higher collection preference and lower collection speed than manufacturer's recommendations. Platelet count decreased from 2380 × 109/L to 1035 × 109/L in the first procedure and from 1813 × 109/L to 768 × 109 in the second procedure. Platelet collection efficiency was calculated to be 110.3% and 86.1% in the first and second thrombocytapheresis, respectively. Therapeutic thrombocytapheresis with Spectra Optia is a safe and efficient therapy to treat patients with primary thrombocytosis while effect of cytoreductive agents is attained. Platelet collection efficiency was calculated to be higher than previously reported. We suggest that changes in technical parameters such as a deeper aspiration point and/or lower collection speed may increase procedure's efficiency.
Downregulation of HDAC2 and HDAC3 via oleuropein as a potent prevention and therapeutic agent in MCF‐7 breast cancer cells
Authors: Bayat Sahar , Mansoori Derakhshan Sima , Mansoori Derakhshan Neda , Shekari Khaniani Mahmoud , Alivand Mohammad Reza 
Published: January 2019

AbstractBreast cancer is the most common malignancy in the world with the highest rate of morbidity and mortality. Due to the several side effects of chemotherapy and radiotherapy, recent studies have focused on the use of herbal medicines. Epidemiological reports have shown the inverse relationship between breast cancer risk and intake of olive. Oleuropein (OLE) is a polyphenolic compound in virgin olive oil with antineoplastic properties and it is well tolerated by humans. Recent reports have shown that OLE has effects on the control of cancer by modulating epigenetics, such as histone deacetylase (HDAC) inhibition. However, the epigenetic mechanisms of OLE anticancer properties are yet to be properly investigated. Therefore, this study aimed to determine the therapeutic effects of OLE through the modulation of histone deacetylase 2 (HDAC2) and histone deacetylase 3 (HDAC3) expression in breast cancer cell line. MCF‐7 cells were tested with and without OLE, and also the cell viability, apoptosis, and migration were examined. HDAC2 and HDAC3 expression genes were assessed by quantitative real‐time polymerase chain reaction. It was found that OLE decreased the expression of both HDAC2 and HDAC3 (P < 0.05), induced apoptosis and retarded cell migration and cell invasion in a dose‐dependent manner (P < 0.05). These results showed that OLE is a potential therapeutic and preventive agent for breast cancer.
Long noncoding RNA Linc00339 promotes triple‐negative breast cancer progression through miR‐377‐3p/HOXC6 signaling pathway
Authors: Wang Xiaolong , Chen Tong , Zhang Yan , Zhang Ning , Li Chen , Li Yaming , Liu Ying , Zhang Hanwen , Zhao Wenjing , Chen Bing , Wang Lijuan , Yang Qifeng 
Published: January 2019

AbstractRecently, long noncoding RNAs (lncRNAs) have become the key gene regulators and prognostic biomarkers in various cancers. Through microarray data, Linc00339 was identified as a candidate oncogenic lncRNA. We compared the expression levels of Linc00339 in several breast cancer cell lines and normal mammary gland epithelial cell line. The effects of Linc00339 on tumor progression were examined both in vitro and in vivo. 3‐(4,5‐Dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide assays were applied to evaluate the functions of Linc00339, miR‐377‐3p, and HOXC6 on cell proliferation. Flow cytometry analysis was used to detect apoptosis and cell cycle distribution. Overall survival (OS) was analyzed using data from The Cancer Genome Atlas and molecular taxonomy of breast cancer international consortium (METABRIC). Dual luciferase assay and RNA immunoprecipitation were performed to confirm the interaction between Linc003339 and miR‐377‐3p. Linc00339 was increased in breast cancer cell lines compared with the normal epithelial cell. Through in vitro and in vivo experiments, Linc00339 overexpression promoted triple‐negative breast cancer (TNBC) proliferation, inhibited cell cycle arrest, and suppressed apoptosis. Silencing of Linc00339 obtained the opposite effects. Mechanistic investigations demonstrated that Linc00339 could sponge miR‐377‐3p and regulate its expression. Higher expression of miR‐377‐3p indicated longer OS in breast cancer patients, especially in TNBC patients. Overexpression of miR‐377‐3p retarded TNBC cell growth through regulating cell cycle distribution and apoptosis. And miR‐377‐3p was involved in Linc00339‐mediated TNBC proliferation through regulating HOXC6 expression. Knockdown of HOXC6 inhibited TNBC progression. In conclusion, our results illuminated that the novel Linc00339/miR‐377‐3p/HOXC6 axis played a critical role in TNBC progression and might be a promising therapeutic target for TNBC treatment.
The phosphoinositide hydrolase phospholipase C delta1 inhibits epithelial‐mesenchymal transition and is silenced in colorectal cancer
Authors: Xiang Qin , He Xiaoqian , Mu Junhao , Mu Haixi , Zhou Dishu , Tang Jun , Xiao Qian , Jiang Yu , Ren Guosheng , Xiang Tingxiu , Peng Weiyan 
Published: January 2019

AbstractIn this study, we found that the phospholipase C delta1 (PLCD1) protein expression is reduced in colorectal tumor tissues compared with paired surgical margin tissues. PLCD1‐promoted CpG methylation was detected in 29/64 (45%) primary colorectal tumors, but not in nontumor tissues. The
PLCD1 RNA expression was also reduced in three out of six cell lines, due to PLCD1 methylation. The ectopic expression of
PLCD1 resulted in inhibited proliferation and attenuated migration of colorectal tumor cells, yet promoted colorectal tumor cell apoptosis in vitro. We also observed that PLCD1 suppressed proliferation and promoted apoptosis in vivo. In addition,
PLCD1 induced G1/S phase cell cycle arrest. Furthermore, we found that PLCD1 led to the downregulation of several factors downstream of β‐catenin, including c‐Myc and cyclin D1, which are generally known to be promoters of tumorigenesis. This downregulation was caused by an upregulation of E‐cadherin in colorectal tumor cells. Our findings provide insights into the role of
PLCD1 as a tumor suppressor gene in colorectal cancer (CRC), and demonstrate that it plays significant roles in proliferation, migration, invasion, cell cycle progression, and epithelial‐mesenchymal transition. On the basis of these results, tumor‐specific methylation of PLCD1 could be used as a novel biomarker for early detection and prognostic prediction in CRC.
S1P mediates human amniotic cells proliferation induced by a 50‐Hz magnetic field exposure via ERK1/2 signaling pathway
Authors: Qiu Liping , Chen Liangjing , Yang Xiaobo , Ye Anfang , Jiang Wei , Sun Wenjun 
Published: January 2019

AbstractExtremely low frequency electromagnetic field (ELF‐EMF) is a kind of physical stimulus in public and occupational environment. Numerous studies have indicated that exposure of cells to ELF‐EMF could promote cell proliferation. But the detailed mechanisms implicated in these proliferative processes remain unclear. In the present experiment, the possible roles of sphingosine‐1‐phosphate (S1P) in 50‐Hz magnetic field (MF)‐induced cell proliferation were investigated. Results showed that exposure of human amniotic (FL) cells to a 50‐Hz MF with an intensity of 0.4 mT significantly enhanced ceramide metabolism, increased S1P production, activated extracellular signal regulated kinase 1/2 (ERK1/2), and promoted cell proliferation. All of these effects induced by MF exposure could be inhibited by SKI II, an inhibitor of sphingosine kinase (SphK). In addition, both the cell proliferative response and the ERK1/2 activation induced by MF exposure were blocked completely by U0126, a specific inhibitor of MEK (ERK kinases 1 and 2). Taken together, the findings in present study suggested that S1P mediated 50‐Hz MF‐induced cell proliferation via triggering ERK1/2 signal pathway.
Overexpression of RNF187 induces cell EMT and apoptosis resistance in NSCLC
Authors: Fu Zhongming , Yu Wanjun , Wang Huaying , Chen Xiaofei 
Published: January 2019

AbstractOverexpression of RING finger protein 187 (RNF187) was recently revealed to be a driver of several cancers. However, the expression and function of RNF187 in non–small‐cell lung cancer (NSCLC) are still unknown. Here, we uncovered that RNF187 expression was significantly higher in NSCLC samples than in matched normal lung samples at both the messenger RNA (3.55 ± 0.79 vs. 1.74 ± 0.63) and protein (2.85 ± 0.14 vs. 1.24 ± 0.02) levels. By downregulating or upregulating RNF187 expression in NSCLC cells, we showed that elevated RNF187 expression distinctly enhanced the migration, invasion, and colony formation of NSCLC cells. Moreover, we revealed that high level of RNF187 promoted NSCLC progression by inducing cell epithelial to mesenchymal transition (EMT) and apoptosis resistance mainly via activating the mitogen‐activated protein kinase and PI3K signaling. Clinically, we demonstrated that RNF187 expression was positively associated with advanced TNM stage (p = 1.29 × 10
−6), lymph node metastasis (
p = 2.69 × 10
−9), and large tumor size (
p = 0.002). Importantly, NSCLC patients with elevated RNF187 expression related to the short overall survival rate(
p = 1.29, E‐7) and could serve as an independent prognostic factor in NSCLC patients. Thus, elevated RNF187 expression promotes NSCLC development by inducing cell EMT and apoptosis resistance, and RNF187 may be a novel prognostic indicator for NSCLC patients after curative resection.
Potential use of melatonin in skin cancer treatment: A review of current biological evidence
Authors: Pourhanifeh Mohammad Hossein , Mahdavinia Mostafa , Reiter Russel J. , Asemi Zatollah 
Published: January 2019

AbstractSkin cancer, particularly melanoma, is a leading cause of death worldwide. The therapeutic methods for this malignancy are not effective, and due to the side effects of these treatments, applying an appropriate alternative or complementary treatment is important. According to available data, melatonin as the main product of the pineal gland has oncostatic and antitumoral properties. Also, melatonin acts as an anti‐inflammatory and reactive oxygen species inducer agent which suppresses the growth of tumors. It also has apoptosis induction characteristics through regulating signaling pathways, including heat shock protein 70, nuclear factor‐erythroid 2 p45‐related factor 2 and others. Thus, adding melatonin to chemo‐ and radiotherapy may have synergistic therapeutic effects and increase the survival time in patients with skin cancer. Few clinical studies have evaluated the efficacy of melatonin in skin cancer. Based on the related mechanisms, this review discusses about how melatonin may improve outcomes in skin cancer patients.
Tuning the structural properties of CeO2 by Pr and Fe codoping for enhanced visible‐light catalytic activity
Authors: Wen Weijie , Lou Zhencheng , Chen Yanjin , Chen Dingsheng , Tian Shuanghong , Xiong Ya 
Published: January 2019

AbstractBACKGROUNDIron (Fe)3+ has much a smaller ion radius than cerium (Ce)4+ but praseodymium (Pr) ions have a similar radius to Ce ions with the same valence. Hence, it is interesting to tune the structural properties, including lattice deformation, band gap and oxygen vacancy (Ov), of CeO2 by Pr and Fe codoping for enhanced visible‐light catalytic activity.RESULTSA series of Ce1‐x‐yPrxFeyO2‐σ (x = 0∼0.012, y = 0.003∼0.031) nanoparticles were prepared by a simple one‐step sol‐gel method. The structural properties of CeO2, including crystal deformation (cell lattice from 5.4069 to 5.4195), band gap (from 2.20 to 2.93), oxygen vacancies (Ov from 0.15% to 9.03%), and peroxide species (O22− from 0.37% to 1.09%) can be finely tuned by Pr and Fe codoping. Among ten samples, Ce0.982Pr0.003Fe0.015O2‐σ with proper structural properties showed the highest photocatalytic activity towards the degradation of acid orange 7. The first reaction rate constant reaches 0.0687 min−1, 9.3 and 7.7 times that of Ce0.994Pr0.006O2‐σ and Ce0.985Fe0.015O2‐σ, respectively. The mechanism was found to involve ·OH, ·O2− and photogenerated holes.CONCLUSIONThe structural properties of CeO2 can be finely and easily tuned by Pr and Fe codoping. Thus, the photocatalytic activity of CeO2 under visible light irradiation was boosted. © 2019 Society of Chemical Industry
Removal of reduced sulfur compounds from diluted gas streams by iron(III)EDTA in a trickling filter with in situ iron re‐oxidation
Authors: Kasper Pernille L , Feilberg Anders 
Published: January 2019

AbstractBACKGROUNDThe relatively low solubility of reduced sulfur compounds can limit their removal in trickling filters treating large volumes of air at low residence times. This could be overcome by addition of an oxidizing agent. In this study, the oxidative absorption of reduced sulfur compounds – hydrogen sulfide (H2S), methanethiol (CH3SH) and dimethyl sulfide (CH3S2CH3) – in a high‐porosity trickling filter with added iron chelated by ethylenediaminetetraacetic acid (EDTA) was examined. The process was observed online by proton transfer reaction mass spectrometry (PTR‐MS).RESULTSRemoval efficiencies of H2S remained in the range 80–99% for all combinations of operating parameters tested. The removal efficiency of CH3SH was found to be limited compared to this, with removal efficiencies ranging from 10% to 74% and a strong dependence on gas flow rate and residence time was observed; CH3S2CH3 was found to be inactive. The FeEDTA solutions were prepared from ferrous chloride (FeCl2) as solutions of this compound were more stable than solutions prepared from ferric salts. Oxygen present in the gas stream allowed oxidation of Fe2+EDTA to Fe3+EDTA in situ, making regeneration prior to experimental runs unnecessary. To maintain high removal efficiencies during start‐up, pH should be kept above 7.CONCLUSIONChelated iron catalysts could be effectively applied for H2S removal in a one‐step trickling filter process treating low‐concentration (<3.5 ppm) and diluted waste gas streams, which are characteristic for environmental waste treatment facilities and livestock houses. However, removal of other reduced sulfur compounds important for deodorization was found to be limited. © 2019 Society of Chemical Industry
Dilatation of the common channel in pediatric congenital biliary dilatation remaining after radical operation
Authors: Fukuzawa Hiroaki , Akasaka Yoshinobu , Maeda Kosaku 
Published: January 2019

AbstractBackgroundIn congenital biliary dilatation, the protein plug is likely impacted, especially in a dilated common channel. However, nobody has discussed whether this dilatation of common channel remains after radical operation. The aim of this study was to investigate the situation of the dilated common channel after radical operation.MethodsThe status of the common channel was investigated at radical operation and follow‐up. We then evaluated whether dilation of the common channel remained postoperatively. Moreover, the association between status of the common channel and postoperative pancreatic complication was evaluated.ResultsPostoperative condition of the common channel was able to be evaluated ultrasonographically in 42 patients. The common channel was dilated at the time of operation in 18 patients and remained dilated postoperatively in 11 of these 18 patients. Dilatation of the common channel was not identified in any patients without dilatation at the time of operation. No patients in this series showed any postoperative pancreatic complications, even if a dilated common channel remained.ConclusionsDilatation of the common channel can remain postoperatively in congenital biliary dilatation, but does not appear to be associated with postoperative pancreatic complications.
Spatial identification of conservation priority areas for urban ecological land: An approach based on water ecosystem services
Authors: Peng Jian , Wang An , Luo Lingwei , Liu Yanxu , Li Huilei , Hu Yi'na , Meersmans Jeroen , Wu Jiansheng 
Published: January 2019

AbstractHow to effectively prevent land degradation and ecosystem deterioration in the process of urbanization has been the focus of land degradation researches in urban areas. Urban ecological land can be defined as the natural base on which a city relies to ecologically survive. It closely links the social economy with the natural eco‐environment, providing an important integrated approach to resolve the contradiction between urban expansion and natural ecosystems conservation in the process of urbanization. The research question addressed in this study is how to accurately identify the conservation priority areas for urban ecological land. Taking Zhuhai City, located in China, as an example, an approach based on seven kinds of water ecosystem services was put forward, combining social demand and natural supply for the services to determine service targets and conservation priority areas. The results showed that the conservation priority areas in Zhuhai City covered 868 km2, accounting for 51.03% of the total land area, which were mainly covered by woodlands or paddy fields and fish ponds. In addition, by synthesizing ecological importance and ecological sensitivity, management zones for urban ecological land were delineated, including 510 km2 of primary control areas and 358 km2 of secondary control areas. In the supply and demand view of water ecosystem services, this study put forward an integrated ecosystem‐based approach for conservation priority area identification of urban ecological land, aiming to prevent land degradation and achieve urban ecological sustainability.
Silk Fibroin‐Based Films Enhance Rhodamine 6G Emission in the Solid State: A Chemical–Physical Analysis of their Interactions for the Design of Highly Emissive Biomaterials
Authors: Gasymov Oktay K. , Botta Chiara , Ragona Laura , Guliyeva Aytaj J. , Molinari Henriette 
Published: January 2019

AbstractDescribed herein is the preparation of dye‐doped films employing silk fibroin (SF) as a biomaterial, capable of preserving the optical properties of the monomeric dye in the solid state, a critical requisite for optical and biolaser applications. A comprehensive physical–chemical characterization is reported for SF films doped with Rhodamine 6G, an ideal candidate for photonics and optoelectronics. Fourier transform infrared spectroscopy (FTIR), circular dichroism (CD) and X‐Ray diffraction (XRD) provide information on SF secondary conformation in the presence of rhodamine. UV–vis absorption spectra and exciton CD inform on the structure of encapsulated rhodamine, while changes in dye photophysical properties illuminate the molecular mechanism of the involved host–guest interactions. SF host environment inhibits rhodamine dimer formation, indicating that SF is an optimum matrix to keep rhodamine essentially monomeric at concentrations as high as 7 mm in the film. The relevant optical properties of these films and the easiness of their preparation, make these systems optimal candidates for innovative photonic technologies.