bluuebunny/crossref_metadata_2025_split huggingface dataset:
HACE1 is a potential tumor suppressor in osteosarcoma
Authors: El-Naggar Amal M , Clarkson Paul W. , Negri Gian Luca , Turgu Busra , Zhang Fan , Anglesio Michael S. , Sorensen Poul H. 
Published: January 2019

AbstractOsteosarcoma is a malignant bone sarcoma characterized by extensive genomic disruption and a propensity for metastatic spread. Osteoid production suggests a close relationship with normal osteoblasts, and the latter are the presumptive cell of origin of this disease. The HACE1 gene, localized to human chromosome 6q21, encodes the HACE1 HECT E3 ligase, a tumor suppressor in diverse tumors that acts in part by targeting the activated form of RAC1 GTPase for proteasomal degradation. Disruption or loss of 6q21 is relatively common in osteosarcomas, and Hace1−/−/Tp53+/− mice frequently develop osteosarcomas, in contrast to Tp53+/− mice, which do not. This suggests an unexplored link between HACE1 loss and osteosarcoma. Here we compared HACE1 expression in normal osteoblasts and osteosarcoma cell lines in vitro by western blotting and quantitative RT-PCR, and in human osteosarcoma specimens by immunohistochemistry. Both HACE1 transcript and protein levels were reduced in osteosarcoma compared to osteoblasts in vitro. Reduced HACE1 expression in osteosarcoma tumors was observed in 76% of cases and associated with high-grade lesions. Further, clonally derived pairs of high and low metastatic osteosarcoma cell lines showed significant downregulation in the high compared to corresponding low metastatic cells. Ectopic expression of HACE1 markedly inhibited anchorage-independent growth and cell motility of HACE1 osteosarcoma cell lines, and was associated with reduced RAC1 activation and decreased reactive oxygen species (ROS). Finally, HACE1 overexpression blocked osteosarcoma xenograft growth and dramatically reduced pulmonary metastases. These findings point to a potential tumor suppressor function for HACE1 in osteosarcoma.
Novel neuroblastoma amplified sequence (NBAS) mutations in a Japanese boy with fever-triggered recurrent acute liver failure
Authors: Ono Sahoko , Matsuda Junko , Watanabe Etsuko , Akaike Hiroto , Teranishi Hideto , Miyata Ippei , Otomo Takanobu , Sadahira Yoshito , Mizuochi Tatsuki , Kusano Hironori , Kage Masayoshi , Ueno Hiroo , Yoshida Kenichi , Shiraishi Yuichi , Chiba Kenichi , Tanaka Hiroko , Miyano Satoru , Ogawa Seishi , Hayashi Yasuhide , Kanegane Hirokazu , Ouchi Kazunobu 
Published: January 2019

AbstractBiallelic mutations in the neuroblastoma amplified sequence (NBAS) gene have been reported to cause two different clinical spectra: short stature with optic nerve atrophy and Pelger-Huët anomaly (SOPH) syndrome and infantile liver failure syndrome 2 (ILFS2). Here, we describe a case of a 3-year-old Japanese boy who presented with fever-triggered recurrent acute liver failure (ALF). The clinical characteristics were considerable elevation of liver enzymes, severe coagulopathy, and acute renal failure. In addition to the liver phenotype, he had short stature and Pelger-Huët anomaly in the peripheral granulocytes. Whole-exome and Sanger sequencing of the patient and his parents revealed that he carried novel compound heterozygous missense mutations inNBAS, c.1018G>C (p.Gly340Arg) and c.2674 G>T (p.Val892Phe). Both mutations affect evolutionarily conserved amino acid residues and are predicted to be highly damaging. Immunoblot analysis of the patient’s skin fibroblasts showed a normal NBAS protein level but a reduced protein level of its interaction partner, p31, involved in Golgi-to-endoplasmic reticulum retrograde vesicular trafficking. We recommendNBASgene analysis in children with unexplained fever-triggered recurrent ALF or liver dysfunction. Early antipyretic therapy may prevent further episodes of ALF.
In situ atomistic observation of disconnection-mediated grain boundary migration
Authors: Zhu Qi , Cao Guang , Wang Jiangwei , Deng Chuang , Li Jixue , Zhang Ze , Mao Scott X. 
Published: January 2019

AbstractShear-coupled grain boundary (GB) migration is of general significance in the deformation of nanocrystalline and polycrystalline materials, but comprehensive understanding of the migration mechanism at the atomic scale remains largely lacking. Here, we systematically investigate the atomistic migration of Σ11(113) coherent GBs in gold bicrystals using a state-of-art in situ shear testing technique combined with molecular dynamic simulations. We show that shear-coupled GB migration can be realised by the lateral motion of layer-by-layer nucleated GB disconnections, where both single-layer and double-layer disconnections have important contributions to the GB migration through their frequent composition and decomposition. We further demonstrate that the disconnection-mediated GB migration is fully reversible in shear loading cycles. Such disconnection-mediated GB migration should represent a general deformation phenomenon in GBs with different structures in polycrystalline and nanocrystalline materials, where the triple junctions can act as effective nucleation sites of GB disconnections.
Orientation, elastic interaction and magnetic response of asymmetric colloids in a nematic liquid crystal
Authors: Sahu Dinesh Kumar , Anjali Thriveni G. , Basavaraj Madivala G. , Aplinc Jure , Čopar Simon , Dhara Surajit 
Published: January 2019

AbstractColloidal particles in nematic liquid crystals create elastic distortion and experience long-range forces. The symmetry of elastic distortion and consequently the complexity of interaction strongly depends largely on the liquid crystal anchoring, topology and shape of the particles. Here, we introduce a new nematic colloidal system made of peanut-shaped hematite particles. We report experimental studies on spontaneous orientation, mutual interaction, laser assisted self-assembly and the effect of external magnetic fields on the colloids. Majority of the colloids spontaneously orient either parallel or perpendicular to the nematic director. The colloids that are oriented perpendicularly exhibit two types of textures due to the out of plane tilting, which is corroborated by the Landau-de Gennes Q-tensor modelling. The transverse magnetic moment of the peanut-shaped colloids is estimated by using a simple analysis based on the competing effects of magnetic and elastic torques.
Rab11FIP proteins link endocytic recycling vesicles for cytoskeletal transport and tethering
Authors: Machesky Laura M. 
Published: January 2019

Abstract
Regulated trafficking of internalised integrins and growth factor receptors enables polarisation of morphology and motility and enables lumen formation in multicellular structures. Recycling vesicles marked with Rab11 direct internalised cargo back to the plasma membrane to affect biological processes such as polarised trafficking and cancer cell invasion. A recent study by Ji and colleagues, provides insight into how the trafficking protein Rab11FIP2 links with the actin-based motor myo5b and the small GTPase Rab11 to regulate vesicle tethering and transport along actin filaments [1]. The authors used biochemical methods to demonstrate that Rab11a binds directly to the tail of myo5b and that Rab11FIP2 also forms direct interactions with both Rab11a and myo5b tails. These proteins essentially compete for binding to similar regions and thus can regulate the association and activity of each other. Ji and colleagues further demonstrate that Rab11a activates myo5b by binding to its globular tail and relieving a head-tail autoinhibition. Due to differing affinities between Rab11 and myo5b or Rab11FIP2, they propose that Rab11FIP2 mediates the association of myo5b with cargo vesicles, while Rab11a regulates the motor activity of myo5b. The present study thus elucidates how myo5b is regulated by its interactions with Rab11a and Rab11FIP2 and proposes a model for coordination of recycling vesicle tethering and motor activity. The present study has implications for how cells control polarity and motility in health and disease and suggests how Rab11FIP proteins might control motor protein activity and engagement for transport.
Grating ridged waveguide V‐shaped slot array antenna for SATCOM applications
Authors: Yuan Weile , Liang Xianling , Zhang Lina , Geng Junping , Zhu Weiren , Jin Ronghong 
Published: January 2019

This Letter presents a grating ridged waveguide V‐shaped slot array antenna for satellite communication (SATCOM) receiving antenna applications. A special grating ridged waveguide, V‐shaped slots and a matching section are applied in the proposed antenna design, so that the antenna provides an extra filtering response with a high radiation polarisation purity in a compact structure. For demonstration, a referential normal ridged waveguide V‐shaped slot array antenna is designed and compared. The simulation results show that the proposed antenna not only operates well in the working frequencies (12.25–12.75 GHz) but also provides the antenna gains of lower than compared with those of the referential antenna in the interference frequencies (14.0–14.5 GHz).
Risk‐averse energy management system for isolated microgrids considering generation and demand uncertainties based on information gap decision theory
Authors: Nasr Mohamad‐Amin , Nasr‐Azadani Ehsan , Rabiee Abbas , Hosseinian Seyed Hossein 
Published: January 2019

There are many technical challenges for integration of renewable energy sources (RESs) in the context of microgrids. Among these challenges, spinning reserve energy management should be accurately considered in the microgrid scheduling system for a better system operation. This study presents a methodology to model and analyse a novel scheme to integrate RESs, particularly photovoltaic (PV) systems, in diesel generation‐based isolated microgrids. The proposed approach considers the uncertainties of PV power generation and demand, simultaneously, by solving a bi‐level multi‐objective optimisation problem using information gap decision theory (IGDT). The proposed energy management system is formulated considering spinning reserve constraints and the uncertainties associated with PV power generation and load, by solving a unit commitment problem. This method, a non‐probabilistic approach, does not require the probability density function of uncertain parameters and provides a robust framework to better understand the potential savings due to the PV integration. In order to test and perform the analysis, realistic data from a 20 MW hybrid PV project is used as a case study. Furthermore, the proposed method is compared with probabilistic techniques, such as Monte Carlo simulations and scenario‐based stochastic programming technique. The presented studies demonstrate applicability of the proposed model for real microgrids.
Preparation and structural characterisation of magnetic NiFe2O4@ABS@Ag nanocompound with antibacterial property
Authors: Mohebbi Maryam , Allafchian Alireza , Kameli Parviz , Jalali Seyed Amirhoosien 
Published: January 2019

The present research attempted to characterise the structural and magnetic characteristics of NiFe2O4@ABS@Ag nanoparticles (NPs) made of nickel ferrite (NiFe2O4), acrylonitrile butadiene styrene (ABS) and silver NPs (Ag NPs), this was done by employing techniques such as X‐ray diffraction (XRD), Fourier transform infrared spectroscopy, scanning electron microscopy and transmission electron microscopy, as well as a vibrating sample magnetometer. The XRD patterns obtained indicated that following ABS and Ag NPs coating, the crystallite size (from Scherrer formula) was increased from 32 to 40 nm. It was confirmed by the magnetic measurements that the maximum magnetisation of the NiFe2O4 core was dropped when the surface was coated with ABS and Ag NPs. The antimicrobial activity of this three‐component nanocomposite was tested, showing that the nanocomposite NiFe2O4@ABS@Ag could be isolated from a water solution by using a magnet.
Preparation of CQDs with hydroxyl function for Fe3+ detection
Authors: Dong Limin , Zhang Wanqi , Li Xuejiao , Geng Xiaoyao 
Published: January 2019

Owing to its excellent fluorescence stability, unique up‐conversion fluorescence properties and biocompatibility, carbon‐quantum dots (CQDs) have become a research hot spot in the field of carbon materials. This study used a series of different preparation strategies to synthesise CQDs such as microwave method, solvothermal method and hydrothermal method. The prepared quantum dots can be further applied to design and metal ion detection. It is worth noting that the CQDs prepared by the hydrothermal method are evenly distributed in the range of 2–3 nm without any agglomeration. Fluorescence quenching was performed by Fe3+, and the introduction of PO4 3− ion could increase the fluorescence intensity of CQDs‐Fe3+ system. Furthermore, CQDs were hydroxyl functionalised by treating with ethanol. The functionalised CQDs synthesised by solvothermal method greatly enhanced the fluorescence intensity.
IRAM and Gaia views of multi-episodic star formation in IC 1396A
Authors: Sicilia-Aguilar Aurora , Patel Nimesh , Fang Min , Roccatagliata Veronica , Getman Konstantin , Goldsmith Paul 
Published: January 2019

Context. IC 1396A is a cometary globule that contains the Class 0 source IC 1396A-PACS-1, which was discovered with Herschel.
Aims. We use IRAM 30m telescope and Gaia DR2 data to explore the star formation history of IC 1396A and investigate the possibilities of triggered star formation.
Methods. IRAM and Herschel continuum data were used to obtain dust temperature and column density maps. Heterodyne data reveal the velocity structure of the gas. Gaia DR2 proper motions for the stars complete the kinematics of the region.
Results. IC 1396A-PACS-1 presents molecular emission similar to a hot corino with warm carbon chain chemistry due to the UV irradiation. The source is embedded in a dense clump surrounded by gas at velocities that are significantly different from the velocities of the Tr 37 cluster. CN emission reveals photoevaporation, while continuum data and high-density tracers (C18O, HCO+, DCO+, and N2D+) reveal distinct gaseous structures with a range of densities and masses.
Conclusions. By combining the velocity, column density, and temperature information and Gaia DR2 kinematics, we confirm that the globule has experienced various episodes of star formation. IC 1396A-PACS-1 is probably the last intermediate-mass protostar that will form within IC 1396A; it shows evidence of being triggered by radiation-driven implosion. Chemical signatures such as CCS place IC 1396A-PACS-1 among the youngest known protostars. Gaia DR2 data reveal velocities in the plane of the sky ~4 km s−1 for IC 1396A with respect to Tr 37. The total velocity difference (8 km s−1) between the Tr 37 cluster and IC 1396A is too small for IC 1396A to have undergone substantial rocket acceleration, which imposes constraints on the distance to the ionizing source in time and the possibilities of triggered star formation. The three stellar populations in the globule reveal that objects located within relatively close distances (<0.5 pc) can be formed in various star-forming episodes within ~1–2 Myr. Once the remaining cloud disperses, we expect substantial differences in evolutionary stage and initial conditions for the resulting objects and their protoplanetary disks, which may affect their evolution. Finally, evidence for short-range feedback from the embedded protostars, and in particular, the A-type star V390 Cep, is also observed.
Near-infrared spectroscopic indices for unresolved stellar populations
Authors: François P. , Morelli L. , Pizzella A. , Ivanov V. D. , Coccato L. , Cesetti M. , Corsini E. M. , Dalla Bontà E. 
Published: January 2019

Context.A new generation of spectral synthesis models has been developed in recent years, but there is no matching set of template galaxy spectra, in terms of quality and resolution, for testing and refining the new models.Aims.Our main goal is to find and calibrate new near-infrared spectral indices along the Hubble sequence of galaxies which will be used to obtain additional constraints to the population analysis based on medium-resolution integrated spectra of galaxies.Methods.Spectra of previously studied and well-understood galaxies with relatively simple stellar populations (e.g., ellipticals or bulge dominated galaxies) are needed to provide a baseline data set for spectral synthesis models.Results.X-shooter spectra spanning the optical and infrared wavelengths (350–2400 nm) of bright nearby elliptical galaxies with a resolving power ofR ∼ 4000–5400 were obtained. Heliocentric systemic velocity, velocity dispersion, and Mg, Fe, and Hβline-strength indices are presented.Conclusions.We present a library of very-high-quality spectra of galaxies covering a large range of age, metallicity, and morphological type. Such a dataset of spectra will be crucial to addressing important questions of the modern investigation concerning galaxy formation and evolution.
Experimental transition probabilities for 4p – 4d spectral lines in V II
Authors: Nilsson H. , Andersson J. , Engström L. , Lundberg H. , Hartman H. 
Published: January 2019

Aims. We aim to measure lifetimes of levels belonging to the 3d3(4F)4d subconfiguration in V II, and derive absolute transition probabilities by combining the lifetimes with experimental branching fractions.Methods. The lifetimes were measured using time-resolved laser-induced fluorescence in a two-photon excitation scheme. The branching fractions were measured in intensity calibrated spectra from a hollow cathode discharge lamp, recorded with a Fourier transform spectrometer.Results. We report lifetimes for 13 levels at an energy around 73 000 cm−1. Absolute transition probabilities of 78 lines are derived by combining the lifetimes and branching fractions. The experimental values are compared with theoretical data from the literature.
Mesyl phosphoramidate antisense oligonucleotides as an alternative to phosphorothioates with improved biochemical and biological properties
Authors: Miroshnichenko S. K. , Patutina O. A. , Burakova E. A. , Chelobanov B. P. , Fokina A. A. , Vlassov V. V. , Altman S. , Zenkova M. A. , Stetsenko D. A. 
Published: January 2019

Significance
Forty years of research have shown that antisense oligonucleotides have great potential to target mRNAs of disease-associated genes and noncoding RNAs. Among the vast number of oligonucleotide backbone modifications, phosphorothioate modification is the most widely used in research and the clinic. However, along with their merits are notable drawbacks of phosphorothioate oligonucleotides, including decreased binding affinity to RNA, reduced specificity, and increased toxicity. Here we report the synthesis and in vitro evaluation of the DNA analog mesyl phosphoramidate oligonucleotide. This oligonucleotide type recruits RNase H and shows significant advantages over phosphorothioate in RNA affinity, nuclease stability, and specificity in inhibiting key processes of carcinogenesis. Thus, mesyl phosphoramidate oligonucleotides may be an attractive alternative to phosphorothioates.
What does constituent priming mean in the investigation of compound processing?
Authors: Libben Gary , Goral Mira , Baayen R. Harald 
Published: January 2019

Abstract
Most dictionary definitions for the term compound word characterize it as a word that itself
contains two or more words. Thus, a compound word such as goldfish is composed of the constituent words
gold and fish. In this report, we present evidence that compound words such as
goldfish might not contain the words gold and fish, but rather positionally
bound compound constituents (e.g., gold- and -fish) that are distinct and often in competition
with their whole word counterparts. This conceptualization has significant methodological consequences: it calls into question the
assumption that, in a traditional visual constituent priming paradigm, the participant can be said to be presented with
constituents as primes. We claim that they are not presented with constituents. Rather, they are presented with competing
free-standing words. We present evidence for the processing of Hebrew compound words that supports this perspective by revealing
that, counter-intuitively, prime constituent frequency has an attenuating effect on constituent priming. We relate our findings to
previous findings in the study of German compound processing to show that the effect that we report is fundamentally morphological
rather than positional or visual in nature. In contrast to German in which compounds are always head-final morphologically, Hebrew
compounds are always head initial. In addition, whereas German compounds are written as single words, Hebrew compounds are always
written with spaces between constituents. Thus, the commonality of patterning across German and Hebrew is independent of visual
form and constituent ordering, revealing, as we claim, core features of the constituent priming paradigm and compound
processing.
Multimodal acquisition of interactive aptitudes
Authors: Geenen Jarret 
Published: January 2019

AbstractIn this article, I detail incremental microgenetic alterations in the development of one particular socio-interactive aptitude: making a relevant interactive contribution. Taking heed ofClark’s (2014)call for the need to reorient our attention to investigate the pragmatics of interaction by accounting for the multiple communicative modes through which this is acccomplished I detail the ways in which parental facilitation and a flexible participatory configuration, made possible by video-conferencing technology, create conditions enabling the agentive re-introduction of a psycho-socially relevant topic. Paramount are the ways in which residual interactive specificities in introduction, co-production and multimodal configurations re-manifest suggesting a more symbiotic relationship between traditional notions of ‘message’ and ‘production’. During the microgenesis of interactive aptitudes, children are not just learningwhatconstitutes psycho-socially relevant topoi, they also acquire an understanding of exactlyhowto make the contribution through multimodal ensembles.