bluuebunny/crossref_metadata_2025_split huggingface dataset:
Kinematic Profile of Visually Impaired Football Players During Specific Sports Actions
Authors: Finocchietti Sara , Gori Monica , Souza Oliveira Anderson 
Published: July 2019

AbstractBlind football, or Football 5-a-side, is a very popular sport amongst visually impaired individuals (VI) worldwide. However, little is known regarding the movement patterns these players perform in sports actions. Therefore, the aim of this study was to determine whether visually impaired players present changes in their movement patterns in specific functional tasks compared with sighted amateur football players. Six VI and eight sighted amateur football players performed two functional tasks: (1) 5 m shuttle test and (2) 60 s ball passing against a wall. The sighted players performed the tests while fully sighted (SIG) as well as blindfolded (BFO). During both tasks, full-body kinematics was recorded using an inertial motion capture system. The maximal center-of-mass speed and turning center-of-mass speed were computed during the 5 m shuttle test. Foot resultant speed, bilateral arm speed, and trunk flexion were measured during the 60 s ball passing test. The results showed that VI players achieved lower maximal and turning speed compared to SIG players (p < 0.05), but BFO were slower than the VI players. The VI players presented similar foot contact speed during passes when compared to SIG, but they presented greater arm movement speed (p < 0.05) compared to both SIG and BFO. In addition, VI players presented greater trunk flexion angles while passing when compared to both SIG and BFO (p < 0.05). It is concluded that VI players present slower speed while running and turning, and they adopt specific adaptations from arm movements and trunk flexion to perform passes.
Gcn5 and Rpd3 have a limited role in the regulation of cell cycle transcripts during the G1 and S phases in Saccharomyces cerevisiae
Authors: Kishkevich A. , Cooke S. L. , Harris M. R. A. , de Bruin R. A. M. 
Published: July 2019

AbstractActivation of cell cycle regulated transcription during the G1-to-S transition initiates S phase entry and cell cycle commitment. The molecular mechanisms involving G1/S transcriptional regulation are well established and have been shown to be evolutionary conserved from yeast to humans. Previous work has suggested that changes to the chromatin state, specifically through histone acetylation, has an important role in the regulation of G1/S transcription in both yeast and human cells. Here we investigate the role of histone acetylation in G1/S transcriptional regulation in the budding yeast Saccharomyces cerevisiae. Our work shows that histone acetylation at specific sites at G1/S target gene promoters peaks at the G1-to-S transition, coinciding with their peak transcription levels. Acetylation at G1/S target promoters is significantly reduced upon deletion of the previously implicated histone acetyltransferase Gcn5, but G1/S cell cycle regulated transcription is largely unaffected. The histone deacetylase Rpd3, suggested to have a role in Whi5-dependent repression, is required for full repression of G1/S target genes in the G1 and S phases. However, in the context of transcriptionally active levels during the G1-to-S transition, this seems to play a minor role in the regulation of cell cycle transcription. Our data suggests that histone acetylation might modulate the amplitude of G1/S cell cycle regulated transcription in Saccharomyces cerevisiae, but has a limited role in its overall regulation.
First marine cryptotephra in Antarctica found in sediments of the western Ross Sea correlates with englacial tephras and climate records
Authors: Di Roberto Alessio , Colizza Ester , Del Carlo Paola , Petrelli Maurizio , Finocchiaro Furio , Kuhn Gerhard 
Published: July 2019

AbstractWe report the discovery of an important new cryptotephra within marine sediments close to Cape Hallett (northern Victoria Land), in the western Ross Sea, Antarctica. The cryptotephra is fully characterized for its texture, mineralogy and major- and trace-element data obtained on single glass shards. On the basis of geochemical composition, the cryptotephra is unequivocally correlated with the proximal deposits of an explosive eruption of the poorly known Mount Rittmann volcano, situated in northern Victoria Land. The cryptotephra is also correlated with a widespread tephra layer, which was erupted in 1254 C.E. and is present in numerous ice-cores and blue ice fields across East and West Antarctica. The characteristics of the tephra indicate that it was produced by a prolonged, moderate energy, mostly hydromagmatic eruption. This is the first time that a cryptotephra has been identified in marine sediments of the Ross Sea and in ice cores. It provides an important new and widespread stratigraphical datum with which the continental cryosphere and marine sedimentological records in Antarctica can be correlated. Moreover, from a purely volcanological point of view, the discovery further confirms the occurrence of a long-lasting, significant explosive eruption from Mount Rittmann in historical times that produced abundant widely dispersed fine ash. The study also highlights the inadequacy of current hazard assessments for poorly known volcanoes such as Mount Rittmann, located at high southern latitudes.
Combined multiphoton imaging and biaxial tissue extension for quantitative analysis of geometric fiber organization in human reticular dermis
Authors: Ueda Maho , Saito Susumu , Murata Teruasa , Hirano Tomoko , Bise Ryoma , Kabashima Kenji , Suzuki Shigehiko 
Published: July 2019

AbstractThe geometric organization of collagen fibers in human reticular dermis and its relationship to that of elastic fibers remain unclear. The tight packing and complex intertwining of dermal collagen fibers hinder accurate analysis of fiber orientation. We hypothesized that combined multiphoton microscopy and biaxial extension could overcome this issue. Continuous observation of fresh dermal sheets under biaxial extension revealed that the geometry of the elastic fiber network is maintained during expansion. Full-thickness human thigh skin samples were biaxially extended and cleared to visualize the entire reticular dermis. Throughout the dermis, collagen fibers straightened with increased inter-fiber spaces, making them more clearly identifiable after extension. The distribution of collagen fibers was evaluated with compilation of local orientation data. Two or three modes were confirmed in all superficial reticular layer samples. A high degree of local similarities in the direction of collagen and elastic fibers was observed. More than 80% of fibers had directional differences of ≤15°, regardless of layer. Understanding the geometric organization of fibers in the reticular dermis improves the understanding of mechanisms underlying the pliability of human skin. Combined multiphoton imaging and biaxial extension provides a research tool for studying the fibrous microarchitecture of the skin.
Effect of Pt doping on the preferred orientation enhancement in FeCo/SiO2 nanocomposite films
Authors: Liu Mei , Hu Linglong , Ma Yue , Feng Ming , Xu Shichong , Li Haibo 
Published: July 2019

AbstractWe prepared FeCoPt/SiO2 thin films by sol-gel spin-coating technique. As-prepared composite films were reduced in hydrogen to induce texture growth. Structural, magnetic property and surface morphology of the films were characterized by X-ray diffraction (XRD), vibrating sample magnetometer (VSM) and scanning electron microscope (SEM). These experimental data indicate that integrated intensity ratio I(200)/I(110) of diffraction peaks (200) and (110) of FeCo firstly increases and then decreases, while the coercivity first decreases and then increases with increasing Pt doping content. The specimen with less Pt doping content has a large I(200)/I(110) value and small coervicity value, which is closely related with strong (200) texture in FeCo thin film. These results indicate that fcc-Pt is also in favor of promoting (200) FeCo texture like Al or Cu elements, and this similar trends of Pt and Al originate from their similar atomic radius and crystal cell volume.
Altered serum acylcarnitine profile is associated with the status of nonalcoholic fatty liver disease (NAFLD) and NAFLD-related hepatocellular carcinoma
Authors: Enooku Kenichiro , Nakagawa Hayato , Fujiwara Naoto , Kondo Mayuko , Minami Tatsuya , Hoshida Yujin , Shibahara Junji , Tateishi Ryosuke , Koike Kazuhiko 
Published: July 2019

AbstractMetabolic disturbance of lipids is a hallmark of nonalcoholic fatty liver disease (NAFLD). In this study, we measured the serum levels of 15 acylcarnitine species of various carbon chain lengths from 2 to 18 in 241 patients with biopsy-proven NAFLD, including 23 patients with hepatocellular carcinoma (HCC), and analyzed the relationship between serum acylcarnitine profile and NAFLD status. Long-chain acylcarnitines AC14:1 and AC18:1 increased gradually with the progression of fibrosis and further increased in patients with HCC, whereas the middle-chain acylcarnitine AC5:0 exhibited the opposite trend. In particular, AC18:1, which we previously showed to possess a tumor promoting effect, was significantly elevated in patients with HCC compared to those without HCC. In addition, long-chain acylcarntines including AC18:1 were positively correlated with serum levels of inflammatory cytokines. Although none of the acylcarnitine species were independently associated with the presence of HCC, (AC16:0 + AC18:1)/AC2:0, an index for the diagnosis of carnitine palmitoyltransferase 2 (CPT2) deficiency, was independently associated with the presence of HCC after adjusting for age and liver fibrosis stage, likely reflecting the downregulation of CPT2 in HCC tissues. Thus, serum acylcarnitine profiles changed significantly according to the status of NAFLD, which may be implicated in the pathogenesis of NAFLD.
Low Expression of miR-424-3p is Highly Correlated with Clinical Failure in Prostate Cancer
Authors: Richardsen E. , Andersen S. , Al-Saad S. , Rakaee M. , Nordby Y. , Pedersen M. I. , Ness N. , Ingebriktsen L. M. , Fassina A. , Taskén K. A. , Mills I. G. , Donnem T. , Bremnes R. M. , Busund L. T. 
Published: July 2019

AbstractProstate cancer (PC) is a highly heterogenous disease and one of the leading causes of mortality in developed countries. Recently, studies have shown that expression of immune checkpoint proteins are directly or indirectly repressed by microRNAs (miRs) in many types of cancers. The great advantages of using miRs based therapy is the capacity of these short transcripts to target multiple molecules for the same- or different pathways with synergistic immune inhibition effects. miR-424 has previously been described as a biomarker of poor prognosis in different types of cancers. miR-424 is also found to target both the CTLA-4/CD80- and PD-1/PD-L1 axis. In the present study, the clinical significance of miR-424-3p expression in PC tissue was evaluated. Naïve radical prostatectomy specimens from 535 patients was used for tissue microarray construction. In situ hybridization was used to evaluate the expression of miR-424-3p and immunohistochemistry was used for CTLA-4 protein detection. In univariate- and multivariate analyses, low expression of miR-424-3p was significant associated with clinical failure-free survival, (p = 0.004) and p = 0.018 (HR:0.44, CI95% 0.22–0.87). Low expression of miR-424-3p also associated strongly with aggressive phenotype of PC. This highlight the importance of miR-424-3p as potential target for therapeutic treatment in prostate cancer.
Circulating levels of PD-L1 and Galectin-9 are associated with patient survival in surgically treated Hepatocellular Carcinoma independent of their intra-tumoral expression levels
Authors: Sideras Kostandinos , de Man Robert A. , Harrington Susan M. , Polak Wojciech G. , Zhou Guoying , Schutz Hannah M. , Pedroza-Gonzalez Alexander , Biermann Katharina , Mancham Shanta , Hansen Bettina E. , Bart Takkenberg R. , van Vuuren Anneke J. , Pan Qiuwei , Ijzermans Jan N. M. , Sleijfer Stefan , Sprengers Dave , Dong Haidong , Kwekkeboom Jaap , Bruno Marco J. 
Published: July 2019

AbstractTumor expression of immune co-inhibitory ligands, such as PD-L1 and Galectin-9, have potential prognostic value in Hepatocellular Carcinoma (HCC). Circulating levels of these molecules, however, have hardly been studied. This study aims to assess the prognostic significance of circulating PD-L1 and circulating Galectin-9 in patients with resected HCC, and to compare their prognostic significance to the intra-tumoral expression of these same molecules. Archived tissues and stored peripheral blood samples from 81 patients who underwent HCC resection or liver transplantation, with curative intent, were used. Immunohistochemistry was performed to determine intra-tumoral expression of PD-L1 and Galectin-9, while ELISA was used to quantify their respective circulating levels. High circulating PD-L1 (HR 0.12, 95%CI 0.16–0.86, p = 0.011) and high circulating Galectin-9 (HR 0.11, 95%CI 0.15–0.85, p = 0.010) levels were both associated with improved HCC-specific survival. Surprisingly, there was no correlation between circulating levels of PD-L1 and Galectin-9 and their intra-tumoral expression levels. In fact, circulating levels of PD-L1 and Galectin-9 were predictive of HCC-specific survival independently of intra-tumoral levels and baseline clinicopathologic characteristics. Combined analysis of circulating levels and intra-tumoral expression of PD-L1 (HR 0.33, 95%CI 0.16–0.68, p = 0.002) and Galectin-9 (HR 0.27, 95%CI 0.13–0.57, p = 0.001) resulted in more confident prediction of survival. In conclusion, circulating PD-L1 and Galectin-9 levels prognostically differentiate resected HCC patients, independently of their intra-tumoral expression. Combining circulating and intra-tumoral expression levels of PD-L1 or Galectin-9 further improves the prognostic values of these immune biomarkers.
High-throughput time-resolved morphology screening in bacteria reveals phenotypic responses to antibiotics
Authors: Zahir Taiyeb , Camacho Rafael , Vitale Raffaele , Ruckebusch Cyril , Hofkens Johan , Fauvart Maarten , Michiels Jan 
Published: July 2019

AbstractImage-based high-throughput screening strategies for quantifying morphological phenotypes have proven widely successful. Here we describe a combined experimental and multivariate image analysis approach for systematic large-scale phenotyping of morphological dynamics in bacteria. Using off-the-shelf components and software, we established a workflow for high-throughput time-resolved microscopy. We then screened the single‐gene deletion collection of Escherichia coli for antibiotic-induced morphological changes. Using single-cell quantitative descriptors and supervised classification methods, we measured how different cell morphologies developed over time for all strains in response to the β-lactam antibiotic cefsulodin. 191 strains exhibit significant variations under antibiotic treatment. Phenotypic clustering provided insights into processes that alter the antibiotic response. Mutants with stable bulges show delayed lysis, contributing to antibiotic tolerance. Lipopolysaccharides play a crucial role in bulge stability. This study demonstrates how multiparametric phenotyping by high-throughput time-resolved imaging and computer-aided cell classification can be used for comprehensively studying dynamic morphological transitions in bacteria.
Revealing the nature of central emission nebulae in the dwarf galaxy NGC 185
Authors: Vučetić M. M. , Ilić D. , Egorov O. V. , Moiseev A. , Onić D. , Pannuti T. G. , Arbutina B. , Petrov N. , Urošević D. 
Published: July 2019

Aims. In this paper we present new optical observations of the galaxy NGC 185 intended to reveal the status of supernova remnants (SNRs) in this dwarf companion of the Andromeda galaxy. Previously, it was reported that this galaxy hosts one SNR.
Methods. Our deep photometric study with the 2m telescope at Rozhen National Astronomical Observatory using narrow-band Hα and [S II] filters revealed complex structure of the interstellar medium in the center of the galaxy. To confirm the classification and to study the kinematics of the detected nebulae, we carried out spectroscopic observations using the SCORPIO multi-mode spectrograph at the 6m telescope at the Special Astrophysical Observatory of the Russian Academy of Science, both in low- and high-resolution modes. We also searched the archival X-ray and radio data for counterparts of the candidate SNRs identified by our optical observations.
Results. Our observations imply the presence of one more SNR, one possible H II region previously cataloged as part of an SNR, and the presence of an additional source of shock ionization in one low-brightness planetary nebula. We detected enhanced [S II]/Hα and [N II]/Hα line ratios, as well as relatively high (up to 90 km s−1) expansion velocities of the two observed nebulae, motivating their classification as SNRs (with diameters of 45 pc and 50 pc), confirmed by both photometric and spectral observations. The estimated electron density of emission nebulae is 30–200 cm−3. Archival XMM-Newton observations indicate the presence of an extended, low-brightness, soft source in projection of one of the optical SNRs, whereas the archival VLA radio image shows weak, unresolved emission in the center of NGC 185.
An ALMA+ACA measurement of the shock in the Bullet Cluster
Authors: Di Mascolo Luca , Mroczkowski Tony , Churazov Eugene , Markevitch Maxim , Basu Kaustuv , Clarke Tracy E. , Devlin Mark , Mason Brian S. , Randall Scott W. , Reese Erik D. , Sunyaev Rashid , Wik Daniel R. 
Published: July 2019

Context. The thermal Sunyaev-Zeldovich (SZ) effect presents a relatively new tool for characterizing galaxy cluster merger shocks, traditionally studied through X-ray observations. Widely regarded as the “textbook example” of a cluster merger bow shock, the western, most-prominent shock front in the Bullet Cluster (1E0657-56) represents the ideal test case for such an SZ study.
Aims. We aim to characterize the shock properties using deep, high-resolution interferometric SZ effect observations in combination with priors from an independent X-ray analysis.
Methods. Our analysis technique relies on the reconstruction of a parametric model for the SZ signal by directly and jointly fitting data from the Atacama Large Millimeter/submillimeter Array (ALMA) and Atacama Compact Array (ACA) in Fourier space.
Results. The ALMA+ACA data are primarily sensitive to the electron pressure difference across the shock front. To estimate the shock Mach number ℳ, this difference can be combined with the value for the upstream electron pressure derived from an independent Chandra X-ray analysis. In the case of instantaneous electron-ion temperature equilibration, we find ℳ = 2.08−0.12+0.12, in   ≈  2.4σ tension with the independent constraint from Chandra, MX = 2.74 ± 0.25. The assumption of purely adiabatic electron temperature change across the shock leads to ℳ = 2.53−0.25+0.33, in better agreement with the X-ray estimate ℳX = 2.57 ± 0.23 derived for the same heating scenario.
Conclusion. We have demonstrated that interferometric observations of the thermal SZ effect provide constraints on the properties of the shock in the Bullet Cluster that are highly complementary to X-ray observations. The combination of X-ray and SZ data yields a powerful probe of the shock properties, capable of measuring ℳ and addressing the question of electron-ion equilibration in cluster shocks. Our analysis is however limited by systematics related to the overall cluster geometry and the complexity of the post-shock gas distribution. To overcome these limitations, a simultaneous, joint-likelihood analysis of SZ and X-ray data is needed.
Does climate opportunity facilitate smallholder farmers’ adaptive capacity in the Sahel?
Authors: Lalou Richard , Sultan Benjamin , Muller Bertrand , Ndonky Alphousseyni 
Published: July 2019

AbstractIn Africa, adaptation will be crucial to offset expected negative climate change impacts on food security and agriculture development. In this study, we combine meteorological data from 18 local stations, field surveys on agricultural practices and agronomic information on the growth of millet to demonstrate the crop suitability to the present climate and the ability of Senegalese farmers to adapt their practices to climate variability, and to disseminate them. From data collected in both 665 villages and 1061 farmers, our study provides quantitative evidence of the responsive adaptation of farmers in the Sahel where the recent resumption of rainfall has provided new agricultural opportunities. Statistical models and cropping simulations show that these farmers innovate by reintroducing and disseminating a long cycle millet cultivar—more suitable for wet environments. We note that although this adaptation is a clear response to recent changes in quantity and distribution of rainfall, its adoption remains limited (50% of the villages visited and 25% of the surveyed agricultural producers have cultivated the new millet variety) and varies strongly within the same climatic context and by characteristics of farmers (willing and capacity), indicating different agricultural strategies (diversification, market exchanges). If land access and development of cash crops are hindrances to the adoption of sanio, poverty is clearly not a barrier and adaptation is not a lever for wealth creation. Such adaptative capacities, together with government incentives for farmers to sustainably adapt to climate change, can be important in reducing climate risks in the coming years.
Emergence of complexity in poetry: “Soleils couchants” by Verlaine
Authors: Choi In-Ryeong , Kim J. W. , Choi M. Y. 
Published: July 2019

AbstractThis work investigates emergence of complexity in poetry based on the analogy between the poetics of evocation and the physics of complex systems. For this purpose, we first discuss key concepts of the physics of complex systems, such as emergence, openness, and large variability. It is then suggested that a poem could be viewed as a representative complex system. Emergence of complexity in poetry is further probed in the context of poetics of evocation. As an example, we demonstrate how poetic complexity can indeed be realized, through the analysis of Verlaine’s poem, “Soleils couchants”. In this way, we propose a remarkable convergence of poetics and physics, yielding meaningful results for both fields. On the side of poetics of evocation, its essential characteristic, “relevant mysteries” that give rise to the great variability of interpretations, is verified; on the side of physics of complex systems, the concepts of complexity is validated to provide further understanding of literature as well as natural and social phenomena.