bluuebunny/crossref_metadata_2025_split huggingface dataset:
The allotetraploid origin and asymmetrical genome evolution of common carp Cyprinus carpio
Authors: Xu Peng , Xu Jian , Liu Guangjian , Chen Lin , Zhou Zhixiong , Peng Wenzhu , Jiang Yanliang , Zhao Zixia , Jia Zhiying , Sun Yonghua , Wu Yidi , Chen Baohua , Pu Fei , Feng Jianxin , Luo Jing , Zhang Hanyuan , Wang Hui , Dong Chuanju , Jiang Wenkai , Sun Xiaowen 
Published: December 2018

AbstractCommon carp (Cyprinus carpio) is an allotetraploid Cyprinid species derived from recent whole genome duplication and provides an excellent model system for studying polyploid genome evolution in vertebrates. To explore the origins and consequences of tetraploidy in C. carpio, we generated three chromosome-level new reference genomes of C. carpio and compared them to the related diploid Cyprinid genome sequences. We identified a progenitor-like diploid Barbinae lineage by analysing the phylogenetic relationship of the homoeologous genes of C. carpio and their orthologues in closely related diploid Cyprinids. We then characterized the allotetraploid origin of C. carpio and divided its genome into two homoeologous subgenomes that are marked by a distinct genome similarity to their diploid progenitor. On the basis of the divergence rates of homoeologous genes and transposable elements in two subgenomes, we estimated that the two diploid progenitor species diverged approximately 23 million years ago (Mya) and merged to form the allotetraploid C. carpio approximately 12.4 Mya, which likely correlated with environmental upheavals caused by the extensive uplift of the Qinghai-Tibetan Plateau. No large-scale gene losses or rediploidization were observed in the two subgenomes. Instead, we found extensive homoeologous gene expression bias across twelve surveyed tissues, which indicates that subgenome B is dominant in homoeologous expression. DNA methylation analysis suggested that CG methylation in promoter regions plays an important role in altering the expression of these homoeologous genes in allotetraploid C. carpio. This study provides an essential genome resource and insights for extending further investigation on the evolutionary consequences of vertebrate polyploidy.
Glioma cell migration in confined microchannels via a motor-clutch mechanism
Authors: Prahl Louis S. , Stanslaski Maria R. , Vargas Pablo , Piel Matthieu , Odde David J. 
Published: December 2018

AbstractGlioma tumor dispersion involves invading cells escaping the tumor bulk and migrating into the healthy brain parenchyma. Here, they encounter linearly aligned track-like tissue structures such as axon bundles and the perivascular space. These environments also contain micrometer-scale pores that impose mechanical confinement on invading cells. To study glioma cell migration in anin vitrosystem that reproduces some of these features, we used microfluidic devices with 60 μm2cross-sectional area channels that confine cells into one-dimensional (1D) tracks. Individual cell tracking revealed strongly persistent migration at a mean rate of 8.5 ± 0.33 nm s-1. Notably, a 1D computational cell migration simulator predicts migration behaviors of glioma cells without significant adjustment of parameters estimated from previous experiments on two-dimensional (2D) substrates. Pharmacological inhibitors of integrin-mediated adhesions, myosin II activation, or drugs targeting F-actin assembly or microtubule dynamics influence migration consistent with simulations where relevant parameters are changed. These results suggest that cell parameters calibrated to a motor-clutch model on 2D substrates effectively predict 1D confined migration behaviorsa priori. Our results outline a method for testing biophysical mechanisms of tumor cell migration in confined spaces and predicting the effects of anti-motility therapy.
Highly multiplexedin situprotein imaging with signal amplification by Immuno-SABER
Authors: Saka Sinem K. , Wang Yu , Kishi Jocelyn Y. , Zhu Allen , Zeng Yitian , Xie Wenxin , Kirli Koray , Yapp Clarence , Cicconet Marcelo , Beliveau Brian J. , Lapan Sylvain W. , Yin Siyuan , Lin Millicent , Boyden Edward S. , Kaeser Pascal S. , Pihan German , Church George M. , Yin Peng 
Published: December 2018

AbstractProbing the molecular organization of tissues requiresin situanalysis by microscopy. However current limitations in multiplexing, sensitivity, and throughput collectively constitute a major barrier for comprehensive single-cell profiling of proteins. Here, we report Immunostaining with Signal Amplification By Exchange Reaction (Immuno-SABER), a rapid, highly multiplexed signal amplification method that simultaneously tackles these key challenges. Immuno-SABER utilizes DNA-barcoded antibodies and provides a method for highly multiplexed signal amplification via modular orthogonal DNA concatemers generated by Primer Exchange Reaction. This approach offers the capability to preprogram and control the amplification level independently for multiple targets withoutin situenzymatic reactions, and the intrinsic scalability to rapidly amplify and image a large number of protein targets. We validated our approach in diverse sample types including cultured cells, cryosections, FFPE sections, and whole mount tissues. We demonstrated independently tunable 5-180-fold amplification for multiple targets, covering the full signal range conventionally achieved by secondary antibodies to tyramide signal amplification, as well as simultaneous signal amplification for 10 different proteins using standard equipment and workflow. We further combined Immuno-SABER with Expansion Microscopy to enable rapid and highly multiplexed super-resolution tissue imaging. Overall, Immuno-SABER presents an effective and accessible platform for rapid, multiplexed imaging of proteins across scales with high sensitivity.
Understanding and recognising the female phenotype of autism spectrum disorder and the “camouflage” hypothesis: a systematic PRISMA review
Authors: Allely Clare Sarah 
Published: December 2018

PurposeFemales with autism spectrum disorder (ASD) may display superficial social skills which may mask their ASD symptomology impacting on the identification of the disorder – known as the “camouflage” hypothesis. Compared to males with ASD, it is increasingly recognised that females with ASD have a stronger ability to imitate behaviour which is socially acceptable, particularly those females who have higher cognitive abilities (i.e. intelligence considered to be within the normal range) (Ehlers and Gillberg, 1993). The paper aims to discuss this issue.Design/methodology/approachThis paper will explore the literature on camouflaging or masking behaviour in females with ASD. A systematic PRISMA review was conducted.FindingsThe capacity to “camouflage” social difficulties in social situations is considered to be one of the main features of the female phenotype of ASD (e.g. Kenyon, 2014). Social imitation or camouflaging enables some level of success and coping, which results in some females never receiving a diagnosis of ASD. They typically may not exhibit any observable functional impairments. However, under the surface of the camouflage, females may experience high levels of subjective stress, anxiety and exhaustion and a need to re-charge or recuperate by withdrawing from any social interaction.Research limitations/implicationsThere is relatively little understanding and knowledge of the female phenotype of ASD. This lack of understanding and knowledge impacts significantly on the ability to identify females with ASD (Laiet al., 2015; Bargielaet al., 2016), which can have a number of negative consequence (Adamouet al., 2018; National Collaborating Centre for Mental Health (UK), 2012).Practical implicationsThere is a need for the development of a camouflaging measure.Originality/valueThere is a real need for further research exploring the positive and negative impact of the phenomenon of “camouflaging”, or “pretending to be normal” in females with ASD.
Achieving service quality and students loyalty through intimacy and trust of employees of universities
Authors: Amegbe Hayford , Hanu Charles , Mensah Farouq 
Published: December 2018

PurposeThe purpose of this paper is to examine the role of employees’ behavioural factors in increasing students’ loyalty. It specifically examines the direct and indirect mediations between employees’ service quality, trust and intimacy. The focus is on the effects of constructs of service quality (SQLTY), trust (TRUS) and intimacy (INTIMACY) on student loyalty (STLOY).Design/methodology/approachThis study depended on a positivist research paradigm. A total of 800 structured questionnaires were administered. However, 743 were retrieved and coded for the final analysis. The analytical tool used in this study is the ADANCO 2.0.1 software and in terms of statistical processing, the PLS–SEM technique was utilised.FindingsThe findings on direct relationships reveal that INTIMACY is a strong predictor of STLOY, whereas service quality (SQLTY) is a predictor of trust (TRUS). The effect of indirect mediation between service quality (SQLTY), trust (TRUS) and intimacy (INTIMACY) on STLOY was not confirmed.Research limitations/implicationsThe study like any academic work has limitations. Despite these limitations, this study offers theoretical as well as practical value for the research community and administrators of universities and higher educational administrators as a whole.Practical implicationsThe study emphasises the critical need for administrators of higher educational institutions to understand that the behaviours of their employees during the service encounter significantly affect intimacy and student loyalty.Originality/valueThe present study is unique because it quantitatively examined how the above-mentioned behavioural factors of employees lead to INTIMACY and STLOY. The use of a university for the research helped to develop a much better explanation of some of the salient considerations for STLOY. The value of this work rests in the complex quantitative relationships studied.
Modeling of upset sensor operation for autonomous unmanned systems applications
Authors: Fagbemi Mofetoluwa , Perhinschi Mario G. , Al-Sinbol Ghassan 
Published: December 2018

Purpose
The purpose of this paper is to develop and implement a general sensor model under normal and abnormal operational conditions including nine functional categories (FCs) to provide additional tools for the design, testing and evaluation of unmanned aerial systems within the West Virginia University unmanned air systems (UAS) simulation environment.

Design/methodology/approach
The characteristics under normal and abnormal operation of various types of sensors typically used for UAS control are classified within nine FCs. A general and comprehensive framework for sensor modeling is defined as a sequential alteration of the exact value of the measurand corresponding to each FC. Simple mathematical and logical algorithms are used in this process. Each FC is characterized by several parameters, which may be maintained constant or may vary during simulation. The user has maximum flexibility in selecting values for the parameters within and outside sensor design ranges. These values can be set to change at pre-defined moments, such that permanent and intermittent scenarios can be simulated. Sensor outputs are integrated with the autonomous flight simulation allowing for evaluation and analysis of control laws.

Findings
The developed sensor model can provide the desirable levels of realism necessary for assessing UAS behavior and dynamic response under sensor failure conditions, as well as evaluating the performance of autonomous flight control laws.

Research limitations/implications
Due to its generality and flexibility, the proposed sensor model allows detailed insight into the dynamic implications of sensor functionality on the performance of control algorithms. It may open new directions for investigating the synergistic interactions between sensors and control systems and lead to improvements in both areas.

Practical implications
The implementation of the proposed sensor model provides a valuable and flexible simulation tool that can support system design for safety purposes. Specifically, it can address directly the analysis and design of fault tolerant flight control laws for autonomous UASs. The proposed model can be easily customized to be used for different complex dynamic systems.

Originality/value
In this paper, information on sensor functionality is fused and organized to develop a general and comprehensive framework for sensor modeling at normal and abnormal operational conditions. The implementation of the proposed approach enhances significantly the capability of the UAS simulation environment to address important issues related to the design of control laws with high performance and desirable robustness for safety purposes.
Optimal path planning of mobile robot using the hybrid cuckoo–bat algorithm in assorted environment
Authors: Gunji Balamurali , B.B.V.L. Deepak , M.B.L. Saraswathi , Mogili Umamaheswara Rao 
Published: December 2018

Purpose
The purpose of this paper is to obtain an optimal mobile robot path planning by the hybrid algorithm, which is developed by two nature inspired meta-heuristic algorithms, namely, cuckoo-search and bat algorithm (BA) in an unknown or partially known environment. The cuckoo-search algorithm is based on the parasitic behavior of the cuckoo, and the BA is based on the echolocation behavior of the bats.

Design/methodology/approach
The developed algorithm starts by sensing the obstacles in the environment using ultrasonic sensor. If there are any obstacles in the path, the authors apply the developed algorithm to find the optimal path otherwise reach the target point directly through diagonal distance.

Findings
The developed algorithm is implemented in MATLAB for the simulation to test the efficiency of the algorithm for different environments. The same path is considered to implement the experiment in the real-world environment. The ARDUINO microcontroller along with the ultrasonic sensor is considered to obtain the path length and time of travel of the robot to reach the goal point.

Originality/value
In this paper, a new hybrid algorithm has been developed to find the optimal path of the mobile robot using cuckoo search and BAs. The developed algorithm is tested with the real-world environment using the mobile robot.
Effectiveness of topical propranolol 4% gel in the treatment of pyogenic granuloma in children
Authors: Mashiah Jacob , Hadj‐Rabia Smail , Slodownik Dan , Harel Avikam , Sprecher Eli , Kutz Ana 
Published: December 2018

AbstractPyogenic granuloma is a benign acquired vascular tumor that affects the skin and mucous membranes, occurring more often in children and young adults. Treatment is often required due to the associated risk of ulceration and bleeding. There are several publications reporting the use of beta‐blockers for the treatment of pyogenic granuloma. The aim of the present study was to evaluate the clinical effectiveness and safety profile of topical propranolol 4% gel for the treatment of pyogenic granuloma. A retrospective study of all cases of pyogenic granuloma treated with topical propranolol 4% gel between 2014 and 2015 was performed. Epidemiological, clinical and treatment data, including effectiveness score and safety, were reviewed. Of a total of 18 patients with pyogenic granuloma, 11 (61.1%) showed complete resolution of the lesion while two (11.1%) had an almost complete response. In three cases (16.6%), the treatment was discontinued due to bleeding, and the lesions were removed by curettage. Local irritation and lack of compliance led to treatment discontinuation in two cases. Altogether, 13 out of 18 patients (72%) had complete or almost complete response to treatment. There was a correlation between treatment duration and response to treatment. One patient only reported local side‐effects including irritation, redness and scaling of the treated area leading to discontinuation of the treatment and curettage of the pyogenic granuloma. No systemic adverse effects were reported. This is an uncontrolled retrospective study. Propranolol 4% gel may be considered as a safe and efficient topical therapy for pyogenic granuloma.
The role of chemosensory protein 10 in the detection of behaviorally active compounds in brown planthopper, Nilaparvata lugens
Authors: Waris Muhammad Irfan , Younas Aneela , Adeel Muhammad Muzammal , Duan Shuang‐Gang , Quershi Sundas Rana , Kaleem Ullah Rana Muhammad , Wang Man‐Qun 
Published: December 2018

AbstractChemosensory proteins (CSPs) play important roles in insects’ chemoreception, although their specific functional roles have not been fully elucidated. In this study, we conducted the developmental expression patterns and competitive binding assay as well as knock‐down assay by RNA interference both in vitro and in vivo to reveal the function of NlugCSP10 from the brown planthopper (BPH), Nilaparvata lugens (Stål), a major pest in rice plants. The results showed that NlugCSP10 messenger RNA was significantly higher in males than in females and correlated to gender, development and wing forms. The fluorescence binding assays revealed that NlugCSP10 exhibited the highest binding affinity with cis‐3‐hexenyl acetate, eicosane, and (+)‐β‐pinene. Behavioral assay revealed that eicosane displayed attractant activity, while cis‐3‐hexenyl acetate, similar to (+)‐β‐pinene significantly repelled N. lugens adults. Silencing of NlugCSP10, which is responsible for cis‐3‐hexenyl acetate binding, significantly disrupted cis‐3‐hexenyl acetate communication. Overall, findings of the present study showed that NlugCSP10 could selectively interrelate with numerous volatiles emitted from host plants and these ligands could be designated to develop slow‐release mediators that attract/repel N. lugens and subsequently improve the exploration of plans to control this insect pest.
The impact of audit quality in rights offerings
Authors: Balachandran Balasingham , Faff Robert , Nguyen Lily H. G. , Puwanenthiren Premkanth 
Published: December 2018

AbstractUsing audit quality as our core focus, we take a fresh look at the tradability and standby status in rights offerings, and the market reaction during the subscription period in the US market. We find that firms with high audit quality are more likely to choose tradable or full standby rights issues. We also find that the subscription period price reaction is positively related to audit quality and negatively related to issue price discount. These results demonstrate an important role played by the choice of auditors in the design of rights offerings, particularly in mitigating the negative price reaction during the subscription period.
Novel BEST1 mutations and special clinical characteristics of autosomal recessive bestrophinopathy in Chinese patients
Authors: Luo Jingyi , Lin Mingkai , Guo Xinxing , Xiao Xueshan , Li Jiali , Hu Huan , Xiao Hui , Xu Xiaoyu , Zhong Yimin , Long Shixian , Luo Guangwei , Mi Lan , Chen Xiangxi , Fang Lei , Wei Wei , Zhang Qingjiong , Liu Xing 
Published: December 2018

AbstractPurposeThe aim of this study was to describe the genetic and clinical characteristics of Chinese patients with autosomal recessive bestrophinopathy (ARB).MethodsThis study presents a retrospective observational case series. Twenty‐one ARB patients and 25 clinically healthy family members were recruited. The coding regions and adjacent intronic regions of BEST1 were analysed via Sanger sequencing. Clinical examinations, including ultrasound biomicroscopy, A‐scan, optical coherence tomography, fundus autofluorescence, fundus fluorescein angiography (FFA), indocyanine green angiography (ICGA) and visual electrophysiology, were reviewed.ResultsSix novel mutations (c.380C>T, p.T127M; c.397A>G, p.N133D; c.500A>G, p.E167G; c.817G>A, p.V273M; c.174_176del, p.Q58del; and c.950_955del, p.S318_L319) and 8 previously reported mutations were identified. The p.R255W mutation had the highest frequency in our cohort. Twenty patients had serous retinal detachment with multifocal subretinal vitelliform deposits in the posterior poles. One patient exhibited chorioretinal atrophy. FFA revealed peripheral vascular leakage in 10 patients, and ICGA revealed hyperfluorescent spots in 8 patients. Visual electrophysiology was abnormal in all patients. Fifteen patients with angle closure (AC) or angle‐closure glaucoma (ACG) had shallower anterior chambers and shorter axial lengths than the patients with open angle, contributing to their risk of developing AC/ACG. One patient developed AC during the 7‐year follow‐up period. The misdiagnosis and missed rates were 35.3% and 58.8%, respectively.ConclusionThe six novel mutations and high frequency of p.R255W suggest ethnical differences in the BEST1 mutation spectrum among Chinese patients. BEST1 gene screening and detailed clinical examinations help establishing a diagnosis of ARB. Clinical evaluations of the risk of developing AC/ACG are recommended for ARB patients.
The future of system justification theory
Authors: Jost John T. , Badaan Vivienne , Goudarzi Shahrzad , Hoffarth Mark , Mogami Mao 
Published: December 2018

In this article, we respond to commentaries by Friesen et al. (2019, Br. J. Soc. Psychol., 58, 315), Osborne et al. (2019, Br. J. Soc. Psychol., 58, 340), and Owuamalam et al. (2019, Br. J. Soc. Psychol., 58, 362) on the occasion of the twenty‐fifth anniversary of system justification theory. The first two commentaries are highly favourable in their evaluation of the state of theory and research on system justification, and they provide insightful suggestions for new directions. The third commentary is far more critical of system justification theory. We address each objection in some detail, seeking to correct a number of misconceptions about system justification theory and clarify the fact that the theory specifies three – ego, group, and system justification – motives rather than one. Finally, we end by proposing exciting new areas for future research, such as (1) distinguishing between subjective and objective consequences of system justification in a broader array of social and political contexts and (2) developing practical interventions to reduce system justification motivation and strengthen the motivation to improve upon the status quo.
Rates and pathways of CH4 oxidation in ferruginous Lake Matano, Indonesia
Authors: Sturm Arne , Fowle David A. , Jones CarriAyne , Leslie Karla , Nomosatryo Sulung , Henny Cynthia , Canfield Donald E. , Crowe Sean A. 
Published: December 2018

AbstractThis study evaluates rates and pathways of methane (CH4) oxidation and uptake using 14C‐based tracer experiments throughout the oxic and anoxic waters of ferruginous Lake Matano. Methane oxidation rates in Lake Matano are moderate (0.36 nmol L−1 day−1 to 117 μmol L−1 day−1) compared to other lakes, but are sufficiently high to preclude strong CH4 fluxes to the atmosphere. In addition to aerobic CH4 oxidation, which takes place in Lake Matano's oxic mixolimnion, we also detected CH4 oxidation in Lake Matano's anoxic ferruginous waters. Here, CH4 oxidation proceeds in the apparent absence of oxygen (O2) and instead appears to be coupled to some as yet uncertain combination of nitrate (), nitrite (), iron (Fe) or manganese (Mn), or sulfate () reduction. Throughout the lake, the fraction of CH4 carbon that is assimilated vs. oxidized to carbon dioxide (CO2) is high (up to 93%), indicating extensive CH4 conversion to biomass and underscoring the importance of CH4 as a carbon and energy source in Lake Matano and potentially other ferruginous or low productivity environments.