bluuebunny/crossref_metadata_2025_split huggingface dataset:
Precipitation regime change in Western North America: The role of Atmospheric Rivers
Authors: Gershunov Alexander , Shulgina Tamara , Clemesha Rachel E. S. , Guirguis Kristen , Pierce David W. , Dettinger Michael D. , Lavers David A. , Cayan Daniel R. , Polade Suraj D. , Kalansky Julie , Ralph F. Martin 
Published: July 2019

AbstractDaily precipitation in California has been projected to become less frequent even as precipitation extremes intensify, leading to uncertainty in the overall response to climate warming. Precipitation extremes are historically associated with Atmospheric Rivers (ARs). Sixteen global climate models are evaluated for realism in modeled historical AR behavior and contribution of the resulting daily precipitation to annual total precipitation over Western North America. The five most realistic models display consistent changes in future AR behavior, constraining the spread of the full ensemble. They, moreover, project increasing year-to-year variability of total annual precipitation, particularly over California, where change in total annual precipitation is not projected with confidence. Focusing on three representative river basins along the West Coast, we show that, while the decrease in precipitation frequency is mostly due to non-AR events, the increase in heavy and extreme precipitation is almost entirely due to ARs. This research demonstrates that examining meteorological causes of precipitation regime change can lead to better and more nuanced understanding of climate projections. It highlights the critical role of future changes in ARs to Western water resources, especially over California.
Does the endometrial cavity have a molecular microbial signature?
Authors: Winters Andrew D. , Romero Roberto , Gervasi Maria Teresa , Gomez-Lopez Nardhy , Tran Maria Rosa , Garcia-Flores Valeria , Pacora Percy , Jung Eunjung , Hassan Sonia S. , Hsu Chaur-Dong , Theis Kevin R. 
Published: July 2019

AbstractRecent molecular studies concluded that the endometrium has a resident microbiota dominated by Lactobacillus spp. and is therefore similar to that of the vagina. These findings were largely derived from endometrial samples obtained through a transcervical catheter and thus prone to contamination. Herein, we investigated the molecular microbial profiles of mid-endometrial samples obtained through hysterectomy and compared them with those of the cervix, vagina, rectum, oral cavity, and controls for background DNA contamination. Microbial profiles were examined through 16S rRNA gene qPCR and sequencing. Universal bacterial qPCR of total 16S rDNA revealed a bacterial load exceeding that of background DNA controls in the endometrium of 60% (15/25) of the study subjects. Bacterial profiles of the endometrium differed from those of the oral cavity, rectum, vagina, and background DNA controls, but not of the cervix. The bacterial profiles of the endometrium and cervix were dominated by Acinetobacter, Pseudomonas, Cloacibacterium, and Comamonadaceae. Both 16S rRNA gene sequencing and Lactobacillus species-specific (L. iners & L crispatus) qPCR showed that Lactobacillus was rare in the endometrium. In conclusion, if there is a microbiota in the middle endometrium, it is not dominated by Lactobacillus as was previously concluded, yet further investigation using culture and microscopy is necessary.
Identification of prognosis markers for endometrial cancer by integrated analysis of DNA methylation and RNA-Seq data
Authors: Huo Xiao , Sun Hengzi , Cao Dongyan , Yang Jiaxin , Peng Peng , Yu Mei , Shen Keng 
Published: July 2019

AbstractEndometrial cancer is highly malignant and has a poor prognosis in the advanced stage, thus, prediction of its prognosis is important. DNA methylation has rapidly gained clinical attention as a biomarker for diagnostic, prognostic and predictive purposes in various cancers. In present study, differentially methylated positions and differentially expressed genes were identified according to DNA methylation and RNA-Seq data. Functional analyses and interaction network were performed to identify hub genes, and overall survival analysis of hub genes were validated. The top genes were evaluated by immunohistochemical staining of endometrial cancer tissues. The gene function was evaluated by cell growth curve after knockdown CDC20 and CCNA2 of endometrial cancer cell line. A total of 329 hypomethylated highly expressed genes and 359 hypermethylated lowly expressed genes were identified, and four hub genes were obtained according to the interaction network. Patients with low expression of CDC20 and CCNA2 showed better overall survival. The results also were demonstrated by the immunohistochemical staining. Cell growth curve also demonstrated that knockdown CDC20 and CCNA2 can suppress the cell proliferation. We have identified two aberrantly methylated genes, CDC20 and CCNA2 as novel biomarkers for precision diagnosis in EC.
An investigation into the role of time-dependent cohesion in interseismic fault restrengthening
Authors: van den Ende M. P. A. , Niemeijer A. R. 
Published: July 2019

AbstractEarthquakes typically exhibit recurrence times that far exceed time-scales attainable in a laboratory setting. To traverse the temporal gap between the laboratory and nature, the slide-hold-slide test is commonly employed as a laboratory analogue for the seismic cycle, from which the time-dependence of fault strength may be assessed. In many studies it is implicitly assumed that all fault restrengthening emanates from an increase in the internal friction coefficient, neglecting contributions from cohesion. By doing so, important information is lost that is relevant for numerical simulations of seismicity on natural faults, as well as for induced seismicity. We conduct slide-hold-slide experiments on granular halite gouge at various normal stresses to assess the time-dependence of the internal coefficient of friction, and of the cohesion, independently of one another. These experiments reveal that both the internal friction coefficient and cohesion increase over time, but that these quantities do not share a common evolution, suggesting different underlying mechanisms.
An array of basic residues is essential for the nucleolytic activity of the PHP domain of bacterial/archaeal PolX DNA polymerases
Authors: Rodríguez Guillermo , Martín María Teresa , de Vega Miguel 
Published: July 2019

AbstractBacterial/archaeal family X DNA polymerases (PolXs) have a C-terminal PHP domain with an active site formed by nine histidines and aspartates that catalyzes 3′-5′ exonuclease, AP-endonuclease, 3′-phosphodiesterase and 3′-phosphatase activities. Multiple sequence alignments have allowed us to identify additional highly conserved residues along the PHP domain of bacterial/archaeal PolXs that form an electropositive path to the catalytic site and whose potential role in the nucleolytic activities had not been established. Here, site directed mutagenesis at the corresponding Bacillus subtilis PolX (PolXBs) residues, Arg469, Arg474, Asn498, Arg503 and Lys545, as well as to the highly conserved residue Phe440 gave rise to enzymes severely affected in all the nucleolytic activities of the enzyme while conserving a wild-type gap-filling activity, indicating a function of those residues in DNA binding at the PHP domain. Altogether, the results obtained with the mutant proteins, the spatial arrangement of those DNA binding residues, the intermolecular transference of the 3′-terminus between the PHP and polymerization active sites, and the available 3D structures of bacterial PolXs led us to propose the requirement to a great degree of a functional/structural flexibility to coordinate the synthetic and degradative activities in these enzymes.
Changes to vertebrate tissue stable isotope (δ15N) composition during decomposition
Authors: Keenan Sarah W. , DeBruyn Jennifer M. 
Published: July 2019

AbstractDuring carcass decomposition, tissues undergo biochemical changes: Cells autolyze, enteric microbes ferment cellular products, and tissues degrade. Ultimately, decomposition fluids are released as an ephemeral nitrogen (N) and carbon source to the surrounding environment. However, decomposition fluids are δ15N-enriched relative to body tissues, leading to a disconnect between starting tissue composition and ending fluid composition. It remains largely unknown when or if tissues exhibit δ15N enrichment postmortem despite the importance of tissue stable isotopes to ecologists. To test our hypothesis that tissues would become progressively δ15N-enriched during decay, soft tissues and bone were collected from beaver carcasses at five time points. All soft tissues, including muscle, were significantly δ15N-enriched compared to fresh tissues, but were not as enriched as decomposition fluids. Tissue breakdown is initially dominated by anaerobic autolysis and later by microbe and insect infiltration, and partly explains decay fluid isotopic enrichment. We speculate that after rupture, preferential volatilization of δ15N-depleted compounds (especially ammonia) contributes to further enrichment. These results constrain the timing, rate, and potential mechanisms driving carcass isotopic enrichment during decay, and suggest that found carcasses (e.g., road kill) should be used with caution for inferring trophic ecology as decay can result in significant postmortem δ15N enrichment.
The Bionic Radiologist: avoiding blurry pictures and providing greater insights
Authors: Dewey Marc , Wilkens Uta 
Published: July 2019

AbstractRadiology images and reports have long been digitalized. However, the potential of the more than 3.6 billion radiology examinations performed annually worldwide has largely gone unused in the effort to digitally transform health care. TheBionic Radiologistis a concept that combines humanity and digitalization for better health care integration of radiology. At a practical level, this concept will achieve critical goals: (1) testing decisions being made scientifically on the basis of disease probabilities and patient preferences; (2) image analysis done consistently at any time and at any site; and (3) treatment suggestions that are closely linked to imaging results and are seamlessly integrated with other information. TheBionic Radiologistwill thus help avoiding missed care opportunities, will provide continuous learning in the work process, and will also allow more time for radiologists’ primary roles: interacting with patients and referring physicians. To achieve that potential, one has to cope with many implementation barriers at both the individual and institutional levels. These include: reluctance to delegate decision making, a possible decrease in image interpretation knowledge and the perception that patient safety and trust are at stake. To facilitate implementation of theBionic Radiologistthe following will be helpful: uncertainty quantifications for suggestions, shared decision making, changes in organizational culture and leadership style, maintained expertise through continuous learning systems for training, and role development of the involved experts. With the support of theBionic Radiologist, disparities are reduced and the delivery of care is provided in a humane and personalized fashion.
Efficacy Study of a Social Communication and Self-Regulation Intervention for School-Age Children With Autism Spectrum Disorder: A Randomized Controlled Trial
Authors: Nowell Sallie W. , Watson Linda R. , Boyd Brian , Klinger Laura G. 
Published: July 2019

Purpose

This study aimed to examine the initial efficacy of a parent-assisted blended intervention combining components of Structured TEACCHing and Social Thinking, designed to increase social communication and self-regulation concept knowledge in 1st and 2nd graders (
n
= 17) diagnosed with autism spectrum disorder (ASD) and their parents.

Method
A randomized delayed treatment control group design with pre- and postintervention assessments of both parents and children was implemented within a community practice setting. Two follow-up assessments at 3 and 6 months postintervention were also completed.

Results
Overall, results indicate that the intervention is efficacious in teaching social communication and self-regulation concept knowledge to children with ASD and their parents. Both parents and children demonstrated an increase in social communication and self-regulation knowledge after participating in the Growing, Learning, and Living With Autism Group as compared to a delayed treatment control group. The effects of the intervention did not extend to parent–child interactions coded from video recordings. Child treatment effects were maintained at the 3- and 6-month follow-up assessments.

Conclusions
Preliminary efficacy of the Growing, Learning, and Living With Autism Group was established. Based on parent report at the conclusion of the intervention, this is a socially valid intervention for teaching social communication and self-regulation skills to school-age children with ASD.

Supplemental Material

https://doi.org/10.23641/asha.8637236
Spectroscopic binaries RV Tauri and DF Cygni
Authors: Manick Rajeev , Kamath Devika , Van Winckel Hans , Jorissen Alain , Sekaran Sanjay , Bowman Dominic M. , Oomen Glenn-Michael , Kluska Jacques , Bollen Dylan , Waelkens Christoffel 
Published: July 2019

Context. Some RV Tauri stars show a long-term photometric variability in their mean magnitudes. DF Cygni (DF Cyg), the only RV Tauri star in the original Kepler field, and the prototype RV Tauri (RV Tau) are two such stars.
Aims. The focus of this paper is on two famous but still poorly understood RV Tauri stars: RV Tau and DF Cyg. We aim to confirm their suspected binary nature and derive their orbital elements to investigate the impact of their orbits on the evolution of these systems. This research is embedded in a wider endeavour to study binary evolution of low- and intermediate-mass stars.
Methods. The high amplitude pulsations were cleaned from the radial-velocity data to better constrain the orbital motion, allowing us to obtain accurate orbital parameters. We also analysed the photometric time series of both stars using a Lomb-Scargle periodogram. We used Gaia Data Release 2 (DR2) parallaxes in combination with the spectral energy distributions (SEDs) to compute their luminosities. These luminosities were complemented with the ones we computed using a period-luminosity-colour (PLC) relation for RV Tauri stars. The ratio of the circumstellar infrared (IR) flux to the photospheric flux obtained from the SEDs was used to estimate the orbital inclination of each system.
Results. DF Cyg and RV Tau are binaries with spectroscopic orbital periods of 784 ± 16 days and 1198 ± 17 days, respectively. These orbital periods are found to be similar to the long-term periodic variability in the photometry, indicating that binarity indeed explains the long-term photometric variability. The SEDs of these systems indicate the presence of a circumbinary disc. Our line of sight grazes the dusty disc, which causes the photometric flux from the star to extinct periodically with the orbital period. Our derived orbital inclinations enabled us to obtain accurate companion masses for DF Cyg and RV Tau, and these were found to be 0.6 ± 0.1 M⊙ and 0.7 ± 0.1 M⊙, respectively. The derived luminosities suggest that RV Tau is a post asymptotic giant branch (post-AGB) binary, while DF Cyg is likely a post red giant branch (post-RGB) binary. Analysis of the Kepler photometry of DF Cyg revealed a power spectrum with side lobes around the fundamental pulsation frequency. This modulation corresponds to the spectroscopic orbital period and hence to the long-term photometric period. Finally we report on the evidence of high velocity absorption features related to the Hα profile in both objects, indicating outflows launched from around the companion.
Endoskopische Vakuumtherapie bei infizierter walled-off Pankreasnekrose
Authors: Triller Carsten 
Published: July 2019

ZusammenfassungDie nekrotisierende Pankreatitis ist nach wie vor eine therapeutische Herausforderung. 38 % der Fälle münden in ein frühes Organversagen, 15 % führen zum Tod. Interventionen werden in 38 % der Fälle nötig 1. Aufgrund geringerer Mortalität gegenüber der klassischen offenen chirurgischen Vorgehensweise sind endoskopische und chirurgische minimalinvasive Methoden etabliert 2. Die endoskopische Vakuumtherapie kann hier eine Ergänzungsmethode darstellen, zu der bisher nur wenige Fallbeschreibungen existieren. Ein fester Stellenwert gegenüber den Standardverfahren wie endoskopischer Nekrosektomie mit Lavage besteht derzeit noch nicht (vgl. ESGE-Guideline „Endoscopic management of acute necrotizing pancreatitis“, 2018; Abschnitt 4.2.8) 1.Der vorliegende Fall beschreibt eine erfolgreiche endoskopische Vakuumtherapie bei infizierter walled-off Pankreasnekrose unter Verwendung einer modifizierten Drainagefolie in Ergänzung zur Standardmethode der endoskopischen Nekrosektomie.
Effects of Various Work-to-rest Ratios during High-intensity Interval Training on Athletic Performance in Adolescents
Authors: Seo Myong-Won , Lee Jung-Min , Jung Hyun Chul , Jung Sung Woo , Song Jong Kook 
Published: July 2019

AbstractTo examine the effect of high-intensity interval training (HIIT) with different work-to-rest ratios on athletic performance in athletes. Forty-seven male Taekwondo athletes (aged 15–18 yrs) were randomly assigned into 3 HIIT groups and a control group. Each group performed 6 and 8 bouts of HIIT: 1) 1:2 (30:60 s), 2) 1:4 (30:120 s), and 3) 1:8 (30:240 s) groups while the control group performed only Taekwondo training program. All HIIT groups completed 10 sessions over 4 weeks. Athletic performance tests including VO2max test, Wingate anaerobic test, vertical jump, and agility T-test were measured at both pre- and post-tests. Two-way repeated measures ANOVA were applied to examine the performance changes between protocols. VO2max improved significantly in all HIIT groups (p<0.01), and the post-hoc test indicated that the only 1:4 group showed significant improvement compared to the control group. The HIIT with 1:4 ratio showed the effective protocol for enhancing anaerobic capacity including relative peak and mean power compared to control (p<0.01). Ten sessions of HIIT involving the 1:4 group, lasting over a brief 4-week period revealed the effective protocol for enhancing both aerobic and anaerobic capacity. Our findings provide practical implications to develop a performance-enhancing program specialized for adolescent Taekwondo athletes.
Outcomes in Patients with a Calipered Kinematically Aligned TKA That Already Had a Contralateral Mechanically Aligned TKA
Authors: Shelton Trevor J. , Gill Manpreet , Athwal Gurbir , Howell Stephen M. , Hull Maury L. 
Published: July 2019

AbstractPrior studies suggest kinematically aligned (KA) total knee arthroplasty (TKA) provides some clinical benefits. There are no reports of self-reported outcome measures in patients treated with a calipered KA TKA that already had a contralateral mechanically aligned (MA) TKA. We performed a retrospective study and asked the following questions: (1) Were you satisfied with your MA TKA when you were treated with the KA TKA? (2) What are the Forgotten Joint Scores (FJS) and Oxford Knee Scores (OKS) in each of your knees? (3) Do you favor one knee? and (4) Did one knee recover faster? From January 2013 to January 2017, 2,378 consecutive primary TKAs were performed of which all were treated with calipered KA that uses serial verification checks incorporating measurements of bone resections and positions to restore the prearthritic or native joint lines accurately. A records review identified patients with a prior primary MA TKA in the contralateral limb. Excluded were those with a history of fracture, osteotomy, infection, or revision knee surgery in either limb. In September 2018, 78 patients (57 females) with a mean age of 73 years (range, 50–91 years) completed a follow-up evaluation consisting of the FJS and OKS questionnaires and three anchor questions. A total of 83% of patients were satisfied with the MA TKA and 92% were satisfied with the KA TKA. The KA TKA had a 15 point higher median FJS and a comparable OKS to that of the MA TKA. Also, 56% of patients favored the KA TKA, and 8% favored the MA TKA. Seventy four percent of patients favored the recovery of the KA TKA, and 6% favored the recovery of the MA TKA. Accordingly, a patient considering a contralateral KA TKA can expect that more often than not the KA TKA will have a higher FJS, a similar OKS, be their favorite knee, and recover faster. Present study is therapeutic and reflects level IV evidence.
In-Hospital Complications following Arthrotomy versus Arthroscopy for Septic Knee Arthritis: A Cohort-Matched Comparison
Authors: Kerbel Yehuda E. , Lieber Alexander M. , Kirchner Gregory J. , Stump Natalie N. , Prodromo John P. , Petrucelli Philip M. , Shah Mitesh P. , Brahmabhatt Shyam 
Published: July 2019

AbstractThere is a paucity of literature comparing the relative merits of open arthrotomy versus arthroscopy for the surgical treatment of septic knee arthritis. The primary goal of this study is to compare the risk of perioperative complications between these two surgical techniques. To this end, 560 patients treated for septic arthritis of the native knee with arthroscopy were statistically matched 1:1 with 560 patients treated with open arthrotomy. The outcome measures included major complications, minor complications, mortality, inpatient hospital charges, and length of stay (LOS). Major complications were defined as myocardial infarction, cardiac arrest, stroke, deep vein thrombosis, pulmonary embolism, pneumonia, postoperative shock, unplanned ventilation, deep surgical site infection, wound dehiscence, infected postoperative seroma, hospital acquired urinary tract infection, and retained surgical item. Minor complications included phlebitis and thrombophlebitis, postprocedural emphysema, minor surgical site infection, peripheral nerve complication, and intraoperative hemorrhage. Mortality data were extracted from the database using the Uniform Bill patient disposition. Complications were analyzed using univariate and multivariate logistic regression models, whereas mean costs and LOS were compared using the Kruskal–Wallis H-test. Major complications occurred in 3.8% of the patients in the arthroscopy cohort and 5.4% of the patients in the arthrotomy cohort (p = 0.20). Too few patients in our sample died to report based on National (Nationwide) Impatient Sample (NIS) minimum reporting standards. Rates of minor complications were similar for the arthroscopy and arthrotomy cohorts (12.5 vs. 13.9%; p = 0.48). Multivariate analysis did not reveal any greater risk of minor or major complication between the two procedures. Inpatient hospital cost was similar for arthroscopy (

= $15,917; standard deviation [SD] = 14,424) and arthrotomy (

= $16,020; SD = 18,665; p = 0.42). LOS was also similar for both arthrotomy (6.78 days, SD = 6.75) and arthroscopy (6.24 days, SD = 5.95; p = 0.23). Patients undergoing arthroscopic treatment of septic arthritis of the knee showed no difference in relative risk of perioperative complications, LOS, or hospital cost compared with patients who underwent open arthrotomy.