bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gene editing can generate fragile bivalents in mouse oocytes
Authors: Manil-Ségalen Marion , Łuksza Małgorzata , Kannaan Joanne , Marthiens Véronique , Lane Simon I.R , Jones Keith T. , Terret Marie-Emilie , Basto Renata , Verlhac Marie-Hélène 
Published: June 2018

AbstractMouse female meiotic spindles assemble from acentriolar MTOCs (aMTOCs) that fragment into discrete foci. These are further sorted and clustered to form spindle poles, thus providing balanced forces for faithful chromosome segregation. To assess the impact of aMTOCs biogenesis on spindle assembly, we genetically induced their precocious fragmentation in mouse oocytes using conditional overexpression of Plk4, a master MTOC regulator. Excessive microtubule nucleation from these fragmented aMTOCs accelerated spindle assembly dynamics. Prematurely formed spindles promoted the breakage of three different fragilized bivalents, generated by the presence of recombined Lox P sites. Reducing the density of microtubules diminished the extent of chromosome breakage. Thus, improper spindle forces can lead to widely described yet unexplained chromosomal structural anomalies with disruptive consequences on the ability of the gamete to transmit an uncorrupted genome.
Regulatory T-cells are required for neonatal heart regeneration
Authors: Li Jiatao , Yang Kevin Y. , Yee Tam Rachel Chun , Chan Vicken W. , Sheng Bao , Hori Shohei , Zhou Bin , Dennis Lo Yuk Ming , Lui Kathy O. 
Published: June 2018

AbstractPrevious work has elegantly demonstrated that, unlike adult mammalian heart, the neonatal heart is able to regenerate after injury from postnatal day (P) 1 to 7. Recently, macrophages are found to be required in the repair process as depletion of which abolishes endogenous regenerative capability of the neonatal heart. Nevertheless, whether innate immunity alone is sufficient for neonatal heart regeneration is obscure. Here, we investigate a hitherto novel role of FOXP3+ regulatory T-cells (Treg) in neonatal heart regeneration. Unlike their wild type counterparts, NOD/SCID mice that are deficient for T-cells but innate immune cells including macrophages fail to regenerate their injured heart as early as P3. In wild type mice, both conventional CD4+ T-cells and Treg are recruited to cardiac muscle within the first week after injury. Treatment with the lytic anti-CD4 antibody that specifically depletes conventional CD4+ T-cells leads to reduced cardiac fibrosis; while treatment with the lytic anti-CD25 antibody that specifically depletes CD4+CD25hiFOXP3+ Treg contributes to increased fibrosis of the neonatal heart after injury. Moreover, adoptive transfer of Treg to NOD/SCID mice results in mitigated fibrosis and increased proliferation and function of cardiac muscle of the neonatal heart after injury. Mechanistically, single cell transcriptomic profiling reveals that Treg are a source of chemokines and cytokines that attract monocytes and macrophages previously known to drive neonatal heart regeneration. Furthermore, Treg directly promote proliferation of both mouse and human cardiomyocytes in a paracrine manner. Our findings uncover an unappreciated mechanism in neonatal heart regeneration; and offer new avenues for developing novel therapeutics targeting Treg-mediated heart regeneration.
Healthspan pathway maps in C. elegans and humans highlight transcription, proliferation/biosynthesis and lipids
Authors: Möller Steffen , Saul Nadine , Cohen Alan A. , Köhling Rüdiger , Sender Sina , Escobar Hugo Murua , Junghanss Christian , Cirulli Francesca , Berry Alessandra , Antal Peter , Adler Priit , Vilo Jaak , Boiani Michele , Jansen Ludger , Repsilber Dirk , Grabe Hans Jörgen , Struckmann Stephan , Barrantes Israel , Hamed Mohamed , Wouters Brecht , Schoofs Liliane , Luyten Walter , Fuellen Georg 
Published: June 2018

SummaryThe molecular basis of aging and of aging-associated diseases is being unraveled at an increasing pace. An extended healthspan, and not merely an extension of lifespan, has become the aim of medical practice. However, a precise definition of health and healthspan is not straightforward, and the causal molecular basis of health “per se” is largely unknown. Here, we define health based on the absence of diseases and dysfunctions. Based on an extensive review of the literature, in particular for humans and C. elegans, we compile a list of features of health and of the genes associated with them. Clusters of these genes based on molecular interaction data give rise to maps of healthspan pathways for humans, featuring the themes transcription initiation, proliferation and cholesterol/lipid processing, and for C. elegans, featuring the themes immune response, mitochondrion and biosynthesis based on genetic and compound intervention data, and lipids, biosynthesis and transcription based on WormBase compound intervention data. Overlaying healthspan-related gene expression data (describing effects of metabolic intervention associated with improvements in health) onto the aforementioned healthspan pathway maps, we observe the downregulation of Notch signalling in humans and of proliferation/cell-cycle in C. elegans. The former reflects the proinflammatory role of the Notch pathway. We identify transcription, proliferation/biosynthesis and lipids as a common theme on the annotation level, and proliferation-related kinases on the gene/protein level. Our literature-based data corpus, including visualization, is available as a reference for future investigations, at http://www.h2020awe.eu/index.php/pathways/.
IFN-γ induces epithelial-to-mesenchymal transition of cancer cells via an unique microRNA processing
Authors: Lo U-Ging , Pong Rey-Chen , Yang Diane , Gandee Leah , Hernandez Elizabeth , Dang Andrew , Lin Chung-Jung , Santoyo John , Ma Shi-Hong , Sonavane Rajni , Huang Jun , Tseng Shu-Fen , Moro Loredana , Arbini Arnaldo A. , Kapur Payal , Raj Ganesh , He Dalin , Lai Chih-ho , Lin Ho , Hsieh Jer-Tsong 
Published: June 2018

ABSTRACTInterferon-γ (IFNγ) is a potent cytokine in modulating tumor immunity and tumoricidal effects. We demonstrate a new function of IFNγ in inducing epithelial-to-mesenchymal transition (EMT) in normal and cancer cells from different cell types. IFNγ activates JAK-STAT signaling pathway leading to the transcription of IFN-stimulated genes (ISGs), such as interferon-induced tetratricopeptide repeat 5 (IFIT5). We unveil a new function of IFIT5 complex in degrading precursor microRNAs (pre-miRNA) that include pre-miR-363 from the miR-106a-363 cluster, as well as pre-miR-101 and pre-miR-128 with a similar 5’-end structure with pre-miR-363. Noticeably, these suppressive miRNAs have similar functions by targeting EMT transcription factors in prostate cancer (PCa) cells. We further demonstrated that IFIT5 plays a critical role in IFNγ-induced cell invasivenessin vitroand lung metastasisin vivo. Clinically, IFIT5 is highly elevated in high-grade PCa and its expression inversely correlates with these suppressive miRNAs. Altogether, this study unveils pro-tumorigenic role of the IFN pathway via a new mechanism of action, which certainly raises concern about its clinical application.
PTK2 regulates the UPS impairment via p62 phosphorylation in TDP-43 proteinopathy
Authors: Lee Shinrye , Jeon Yu-Mi , Kim Seyeon , Kwon Younghwi , Jo Myungjin , Jang You-Na , Lee Seongsoo , Kim Jaekwang , Kim Sang Ryong , Lee Kea Joo , Lee Sung Bae , Kim Kiyoung , Kim Hyung-Jun 
Published: June 2018

AbstractTDP-43 proteinopathy is a common feature in a variety of neurodegenerative disorders including Amyotrophic lateral sclerosis (ALS) cases, Frontotemporal lobar degeneration (FTLD), and Alzheimer’s disease. However, the molecular mechanisms underlying TDP-43-induced neurotoxicity are largely unknown. In this study, we demonstrated that TDP-43 proteinopathy induces impairment in ubiquitin-proteasome system (UPS) evidenced by an accumulation of ubiquitinated proteins and reduction of proteasome activity in neuronal cells. Through kinase inhibitor screening, we identified PTK2 as a suppressor of neurotoxicity induced by UPS impairment. Importantly, PTK2 inhibition significantly reduces ubiquitin aggregates and attenuated TDP-43-induced cytotoxicity inDrosophilamodel of TDP-43 proteinopathy. We further identified that phosphorylation of p62 at serine 403 (p-p62S403), a key component in the autophagic degradation of poly-ubiquitinated proteins, is increased upon TDP-43 overexpression and dependent on activation of PTK2 in neuronal cells. Moreover, expressing a non-phosphorylated form of p62 (p62S403A) significantly represses accumulation of polyubiquitinated proteins and neurotoxicity induced by TDP-43 overexpression in neuronal cells. In addition, inhibition of TBK1, a kinase which phosphorylates S403 of p62, ameliorates neurotoxicity upon UPS impairment in neuronal cells. Taken together, our data suggest that activation of PTK2-TBK1-p62 axis plays a critical role in the pathogenesis of TDP-43 by regulating neurotoxicity induced by UPS impairment. Therefore, targeting PTK2-TBK1-p62 axis may represent a novel therapeutic intervention for neurodegenerative diseases with TDP-43 proteinopathy.
CDK phosphorylation of Xenopus laevis M18BP1 promotes its metaphase centromere localization
Authors: French Bradley T. , Straight Aaron F. 
Published: June 2018

AbstractChromosome segregation requires the centromere, the site on chromosomes where kinetochores assemble in mitosis to attach chromosomes to the mitotic spindle. Centromere identity is defined epigenetically by the presence of nucleosomes containing the histone H3 variant CENP-A. New CENP-A nucleosome assembly occurs at the centromere every cell cycle during G1, but how CENP-A nucleosome assembly is spatially and temporally restricted remains poorly understood. Centromere recruitment of factors required for CENP-A assembly is mediated in part by the three-protein Mis18 complex (Mis18α, Mis18β, M18BP1). Here we show that Xenopus M18BP1 localizes to centromeres during metaphase - prior to CENP-A assembly - by binding to CENP-C using a highly conserved SANTA domain. We find that Cdk phosphorylation of M18BP1 is necessary for M18BP1 to bind CENP-C and localize to centromeres in metaphase. Surprisingly, mutations which disrupt the metaphase M18BP1/CENP-C interaction cause defective nuclear localization of M18BP1 in interphase, resulting in defective CENP-A nucleosome assembly. We propose that M18BP1 may identify centromeric sites in metaphase for subsequent CENP-A nucleosome assembly in interphase.
Multimodal evidence for delayed fear extinction in adolescence and young adulthood
Authors: Morriss Jayne , Christakou Anastasia , van Reekum Carien M. 
Published: June 2018

AbstractPrevious research in rodents and humans points to an evolutionarily conserved profile of blunted fear extinction during adolescence, underpinned by brain structures such as the amygdala and medial prefrontal cortex (mPFC). In this study, we examine age-related effects on the function and structural connectivity of this system in fear extinction in adolescence and young adulthood. Younger age was associated with greater amygdala activity and delayed mPFC engagement to learned threat cues as compared to safety cues. Furthermore, greater structural integrity of the uncinate fasciculus, a white matter tract that connects the amygdala and mPFC, mediated the relationship between age and acceleration of mPFC engagement during extinction. These findings suggest that age-related changes in the structure and function of amygdala-mPFC circuitry may underlie the protracted maturation of fear regulatory processes, rendering younger individuals more vulnerable to anxiety disorders, which emerge during development.
Raising bank loss absorption capacity through equity capital or bail-in debt
Authors: Benink Harald A. 
Published: June 2018

Purpose
Based upon recent statements made by the European Shadow Financial Regulatory Committee, a group of well-known professors coming from ten European countries, during the period 2012-2017, this paper aims to analyze from a European perspective the adequacy and credibility of the proposed framework.

Design/methodology/approach
This paper is a summary and interpretation of statements from the European Shadow Financial Regulatory Committee.

Findings
The authors argue that the credibility of the bail-in mechanism is likely to be limited. Because of this, unexpected losses may not be absorbed by unsecured debt holders. Therefore, there is still a need for relatively high equity capital buffers.

Originality/value
The issue of how to raise loss absorption capacity for banks is prominent on the international policy agenda. International regulators are aiming for a combination of equity capital, typically raised by issuing shares, retaining profits and issuing contingent convertible (CoCo) bonds and bail-in debt where unsecured creditors such as holders of subordinated and common bonds are supposed to take losses in case of a bankruptcy or restructuring of a bank.
Organizational tenure and knowledge-sharing behaviours
Authors: Sarti Daria 
Published: June 2018

PurposeThis paper aims to contribute to research on the broad theme of knowledge-sharing (KS) behaviours and the impact that different organizational tenure may have on them. In this relationship, the relevance of the leader in enhancing sharing dynamics among employees is highlighted.Design/methodology/approachThis study focuses on KS attitudes among employees with different numbers of years spent in the same organization, seen through the theoretical lens of a social exchange perspective. Specifically, the role of the leader in building a social exchange with co-workers is investigated as a managerial lever able to favour KS among a more experienced workforce. This paper starts by considering the relationship between organizational tenure and employees’ KS attitude. Furthermore, the specific role played by the leader–member social exchange with regard to this relation is described. The study’s hypotheses are tested on data collected from employees (N= 150) working in a non-profit organization located in central Italy.FindingsThe results suggest that a negative relationship exists between employees’ organizational tenure and KS attitude. In addition, the critical role of a leader’s support is demonstrated in moderating the effect of different organizational tenures on the KS attitude.Research limitations/implicationsThis paper has both theoretical and managerial implications.Originality/valueThis paper contributes to the research on the important role of leaders in enhancing KS behaviors among co-workers when the number of years in the same organizations is a variable of interest.
Understanding public libraries’ challenges, motivators, and perceptions toward the use of social media for marketing
Authors: Choi Namjoo , Joo Soohyung 
Published: June 2018

PurposeThe purpose of this paper is to identify challenges and motivators to social media adoption for marketing purposes in public libraries and to investigate how these libraries perceive the importance of social media marketing.Design/methodology/approachA nation-wide online survey on public libraries in the USA was conducted to carry out this study, and a total number of 470 responses were used for data analysis.FindingsThis study determined a range of challenges and motivators to implementing social media for marketing in public libraries. The results also showed that public libraries perceive social media as an important tool for their library marketing and intend to increase their use.Practical implicationsThe findings from this study can serve as a guideline for public libraries when employing social media for marketing purposes in their libraries.Originality/valueThis study assessed the current state of social media use for marketing in the public library context, a context that has been under-researched in the literature, from three perspectives: challenges, motivators, and perceptions.
Altmetrics: “big data” that map the influence of New Zealand research
Authors: Konkiel Stacy , Guichard Stephanie 
Published: June 2018

Purpose
Altmetrics can offer organizations a unique opportunity to understand the non-traditional scholarly and public influence of their institutions’ research. This paper aims to look at bibliometrics and altmetrics for New Zealand research published in 2016 to understand the country’s research’s reach in social media, mainstream media and public policy, as well as more traditional measures of research impact such as university rankings, citations and publications.

Design/methodology/approach
Research insights platform Dimensions was searched for author affiliations and publication dates for papers published in 2016 by New Zealand researchers (n = 10,934). The study then used Dimensions to perform citation analysis and Altmetric Explorer to find altmetrics for these journal articles, and to generate visualizations to better interrogate the data set.

Findings
Of the 10,934 papers published in 2016 by New Zealand (2016 NZ) researchers, 5,413 (49.5 per cent) were mentioned 86,915 times in one of the 16 sources that Altmetric tracks. Twitter, news outlets and Facebook were among the sources that showed the most engagement with New Zealand 2016 research. Citation analysis tools in Dimensions showed that New Zealand 2016 research had a higher than average Field Citation Ratio (1.51) and Relative Citation Ratio (1.29).

Originality/value
This study combines traditional bibliometric analysis with altmetrics to find new insights into the impact of recent New Zealand research. It suggests new means for organizations to demonstrate the value of the research they produce.
The established state and patient X’s rebellion
Authors: Voyce Andrew 
Published: June 2018

Purpose
The purpose of this paper (anonymised case study) is to explore political perspectives on the detention of patients under the Mental Health Act.

Design/methodology/approach
The approach is ethnographic and narrative.

Findings
The essay offers an exploratory explanation using political theory, of a violent and rebellious act by a person detained as a formal mental health patient. The protest relates to the treatment offered to the patient.

Research limitations/implications
This essay offers a new explanation for a protest for a person detained as a compulsory mental health patient. The essay explores issues relating to political philosophy that the patient applies to their detention.

Practical implications
An understanding of how a patient with a background in academic politics is related in this essay. There is consideration of how an education in politics can be as valid in wellbeing, as a medical degree. It may be that more patients will be spared multiple hospital admissions by the use of effective therapies.

Social implications
There is consideration of the debate about the fitness of current mental health legislation to enable wellbeing, and the debate about the review of mental health law begun in 2017.

Originality/value
This is a perspective of how political theory can inform individual acts. The political inquiry is not of dogma or ideology, either critical or affirming. The discourse is of rebellion with a purpose, not of revolution, class war or national dispute. However, aspects of works that are critical of psychiatry are included in the considerations.
Evaluating the temporal dimension of legitimisation strategies
Authors: Maroun Warren 
Published: June 2018

Purpose
The purpose of this study is to examine how social disclosures by one of the world’s largest producers of Platinum Group Metals are used to maintain and repair legitimacy in the context of South Africa’s prevailing socio-economic conditions and in response to the immediate challenge to legitimacy posed by violent worker demonstrations taking place at its operations in Marikana during August 2012. This is done to highlight how legitimacy strategies take account of the temporal characteristics of a threat to legitimacy and how these, in turn, may constrain the need for far-reaching organisational change.

Design/methodology/approach
Suchman’s (1995) outline of legitimacy theory and Laughlin’s (1991) model of organisational change provide a frame of reference for a detailed thematic content analysis which identifies the use of different strategies by an organization to respond to threats to its credibility and how these impact, resulting changes to business philosophies, policies and systems.

Findings
The study highlights the temporal dimension of legitimisation strategies. Social-related disclosures provided by the case entity in response to labour unrest are aimed at addressing both the episodic and continual threat to legitimacy resulting from the unfavourable event. These also have the effect of limiting the extent of internal changes to select business policies and sub-systems. Carefully managing legitimacy allows the case entity to avoid the need to reformulate its business ethos.

Research limitations/implications
The study deals only with a single case organisation. Although the emphasis is on highlighting themes and principles, results are not necessarily applicable in different contexts. Related to this, although the study deals with a major South African mining company, it does not prove the relevance of local cultural differences to the legitimisation process.

Originality/value
The study dispenses with the use of proxies, such as frequencies of disclosures, to demonstrate how organisations use non-financial reporting to secure legitimacy. Instead, it offers a detailed account of how different sub-sets of legitimacy are being mobilised in corporate reports response to long-term and episodic legitimacy considerations. In addition, the study offers one of the first interpretive accounts of how strategies used to manage legitimacy may constrain the potential of a material external shock resulting in internal organisational change. Finally, the study offers one of the first examples of the operation of legitimacy and organisational change theory from the African Continent.
Evidence-based management for today’s “ambidextrous” organizations
Authors: Jackson Nicole C. , Leung Opal M.C. 
Published: June 2018

Purpose
This paper examines how evidence based management (EBM) can help managers build more flexible organizations. In the context of this article, we define the need to build for this capacity around the challenge of “ambidexterity”, or the need for companies to continue operations while also allowing for innovation. We present a framework to help managers create strategies that help them build ambidexterity in their organizations, whether they operate in highly regulated, compliance driven or un-regulated, non-compliance climates.

Design/methodology/approach
This paper identifies four organizational design strategies each of which represents a different leadership and organization consideration that may focus on how evidence based management practices are linked to competency building (i.e., exploitation), the need innovation, or an equal balance between the two (i.e., ambidexterity).

Findings
Our findings reveal that an organization’s use of data given these four strategic orientations reflect different uses of data (verifiability and codification concerns) and ways of embedding compliance and ambidexterity (exploitation vs. exploration) considerations.

Practical implications
These four strategies help managers expose biases in their current decision-making practices, and how they subsequently may affect lifecycle, change management, and data practice in ambidexterity development.

Originality/value
While EBM acknowledges the importance of utilizing evidence, it remains limited toward understanding how it might be used to build for ambidexterity in organizations.