bluuebunny/crossref_metadata_2025_split huggingface dataset:
Body weight changes and incidence of cachexia after stroke
Authors: Scherbakov Nadja , Pietrock Charlotte , Sandek Anja , Ebner Nicole , Valentova Miroslava , Springer Jochen , Schefold Joerg C. , von Haehling Stephan , Anker Stefan D. , Norman Kristina , Haeusler Karl Georg , Doehner Wolfram 
Published: January 2019

AbstractBackgroundBody weight loss is a frequent complication after stroke, and its adverse effect on clinical outcome has been shown in several clinical trials. The purpose of this prospective longitudinal single‐centre observational study was to investigate dynamical changes of body composition and body weight after ischemic stroke and an association with functional outcome.MethodsSixty‐seven consecutive patients (age 69 ± 11 years, body mass index 27.0 ± 4.1 kg/m2, 42% female patient, mean ± SD) with acute ischemic stroke with mild to moderate neurological deficit (National Institute of Health Stroke Scale median 4, ranged 0–12) were analysed in the acute phase (4 ± 2 days) and at 12 months (389 ± 26 days) follow‐up. Body composition was examined by dual energy X‐ray absorptiometry. Cachexia was defined according to the consensus definition by body weight loss ≥5% within 1 year and additional clinical signs. Lean tissue wasting was considered if a ratio of upper and lower limbs lean mass sum to squared height (kg/m2) was ≤5.45 kg/m2 for female patient and ≤7.25 kg/m2 for male patient.ResultsAccording to the body weight changes after 12 months, 42 (63%) patients had weight gain or stable weight, 11 (16%) patients had moderate weight loss, and 14 (21%) patients became cachectic. A relative decline of 19% of fat tissue and 6.5% of lean tissue was observed in cachectic patients, while no changes of lean tissue were observed in non‐cachectic patients after 12 months. The modified Rankin Scale was 48% higher (2.1 ± 1.6, P < 0.05), Barthel Index was 22% lower (71 ± 39, P < 0.01), and handgrip strength was 34% lower (21.9 ± 13.0, P < 0.05) in cachectic compared to non‐cachectic patients after 12 months.The low physical performance if defined by Barthel Index <60 points was linked to the lean tissue wasting (OR 44.8, P < 0.01), presence of cachexia (OR 20.8, P < 0.01), and low body mass index <25 kg/m2 (OR 11.5, P < 0.05). After adjustment for cofounders, lean tissue wasting remained independently associated with the low physical performance at 12 months follow‐up (OR 137.9, P < 0.05).ConclusionsIn this cohort study, every fifth patient with ischemic stroke fulfilled the criteria of cachexia within 12 months after index event. The incidence of cachexia was 21%. Cachectic patients showed the lowest functional and physical capacity.
Choline‐related‐inherited metabolic diseases—A mini review
Authors: Wortmann Saskia B. , Mayr Johannes A. 
Published: January 2019

AbstractIn humans, the important water soluble, vitamin‐like nutrient choline, is taken up with the diet or recycled in the liver. Deficiencies of choline have only been reported in experimental situations or total parenteral nutrition. Currently, no recommended dietary allowances are published; only an adequate daily intake is defined. Choline is involved in three main physiological processes: structural integrity and lipid‐derived signaling for cell membranes, cholinergic neurotransmission, and methylation. Choline is gaining increasing public attention due to studies reporting a relation of low choline levels to subclinical organ dysfunction (nonalcoholic fatty liver or muscle damage), stunting, and neural tube defects. Furthermore, positive effects on memory and a lowering of cardiovascular risks and inflammatory markers have been proposed. On the other hand, dietary choline has been associated with increased atherosclerosis in mice. This mini review will provide a summary of the biochemical pathways, in which choline is involved and their respective inborn errors of metabolism (caused by mutations in SLC5A7, CHAT, SLC44A1, CHKB, PCYT1A, CEPT1, CAD; DHODH, UMPS, FMO3, DMGDH, and GNMT). The broad phenotypic spectrum ranging from malodor, intellectual disability, to epilepsy, anemia, or congenital myasthenic syndrome is presented, highlighting the central role of choline within human metabolism.
Disorders of riboflavin metabolism
Authors: Balasubramaniam Shanti , Christodoulou John , Rahman Shamima 
Published: January 2019

AbstractRiboflavin (vitamin B2), a water‐soluble vitamin, is an essential nutrient in higher organisms as it is not endogenously synthesised, with requirements being met principally by dietary intake. Tissue‐specific transporter proteins direct riboflavin to the intracellular machinery responsible for the biosynthesis of the flavocoenzymes flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD). These flavocoenzymes play a vital role in ensuring the functionality of a multitude of flavoproteins involved in bioenergetics, redox homeostasis, DNA repair, chromatin remodelling, protein folding, apoptosis, and other physiologically relevant processes. Hence, it is not surprising that the impairment of flavin homeostasis in humans may lead to multisystem dysfunction including neuromuscular disorders, anaemia, abnormal fetal development, and cardiovascular disease. In this review, we provide an overview of riboflavin absorption, transport, and metabolism. We then focus on the clinical and biochemical features associated with biallelic FLAD1 mutations leading to FAD synthase deficiency, the only known primary defect in flavocoenzyme synthesis, in addition to providing an overview of clinical disorders associated with nutritional deficiency of riboflavin and primary defects of riboflavin transport. Finally, we give a brief overview of disorders of the cellular flavoproteome. Because riboflavin therapy may be beneficial in a number of primary or secondary disorders of the cellular flavoproteome, early recognition and prompt management of these disorders is imperative.
Laboratory‐based investigation of fever with rash cases in the Maharashtra State ‐ India, 2014 to 2017
Authors: Vaidya Sunil R. , Kamble Madhukar B. , Kumbhar Neelakshi S. 
Published: January 2019

AbstractThe Maharashtra State (MS), India, launched measles surveillance in the year 2013. From 2014 to 2017, specimens from the patients presenting with fever and skin rashes were received at the National Institute of Virology, Pune. From 36 districts of the MS, 2795 cases (1428 males and 1367 females) were referred for the laboratory diagnosis of measles and rubella using immunoglobulin M enzyme immunoassay and/or RT‐PCRs. The majority of the cases (93.3%, n = 2609) were under 15 years of age. About 17.7% (494) cases had a history of measles immunization (one dose) during their childhood. Virus isolation was attempted from 107 throat swabs and 84 urine samples obtained from 191 cases using Vero hSLAM cells. The results confirmed measles in 1756 and rubella in 282 fever with rash cases by serological and molecular tools. Precisely, 170 of 382 and 35 of 149 specimens were positive for measles and rubella RT‐PCRs, respectively. Sequencing of the representative PCR products showed the circulation of measles virus genotypes D4 (n = 26) and D8 (n = 107), and rubella virus genotype 2B (n = 1). Twenty‐three measles viruses were isolated and genotyped, of which, 6 were D4 and 17 were D8 genotypes. Amongst the measles‐immunized individuals, 51.2% (253/494) had laboratory‐confirmed measles. Overall, 72.9% fever with skin rash cases (n = 2038) from the MS was laboratory confirmed for either measles or rubella. The contribution of measles was higher than rubella in the fever with rash cases. As expected, more fever with rash cases were documented in children compared with adults and highlighted the need to increase measles‐rubella immunization coverage.
Effects of rosemary (Rosmarinus officinalis L.) extracts and dry ice on the physicochemical stability of omega‐3 fatty‐acid‐fortified surimi‐like meat products
Authors: Wang Yu‐Zhu , Wang Sheng‐Yao , Fu Shih‐Guei , Yang Deng‐Jye , Yu Yu‐Shan , Chen Jr‐Wei , Chen Yi‐Chen 
Published: January 2019

AbstractBACKGROUNDLipid peroxidation entails major quality degradation in omega‐3 (ω‐3) fatty‐acid‐fortified surimi‐like meat products upon storage. Currently, the use of label‐friendly alternatives to synthetic antioxidants is encouraged in the industry. Hence, we aimed to examine the applicability of the hurdle‐technology concept, using an 80% (v/v) ethanol solution to obtain rosemary extracts (REs) containing substantial amounts of polyphenol, and dry ice (DI) which can create a cryogenic environment, on the physicochemical stabilities of ω‐3 fatty‐acid (FA)‐fortified meat products after manufacturing and storage periods. The polyphenolic profiles of the REs were also investigated.RESULTSCarnosol and rosmarinic acid are major phenolic components in REs. Furthermore, DI addition during the chopping procedure increased (P < 0.05) whiteness values and hardness of products, while total ω‐3 and ω‐6 FAs were relatively well preserved (P < 0.05) in products with flaxseed oil premixed with RE. During 14‐day storage at 4 °C, combined treatment with RE and DI decreased (P < 0.05) thiobarbituric acid reactive substance (TBARS) levels and the centrifugation loss of products. Single or combined treatment with RE and/or DI decreased (P < 0.05) TBARS levels in products after 60 days of storage at −20 °C.CONCLUSIONDue to the antioxidant‐polyphenol profile of REs and a possible oxygen exclusion of DI treatment under atmospheric pressure during food manufacturing, application of the hurdle‐technology concept, using treatment with both RE and DI, can reduce lipid peroxidation and maintain a greater water‐holding capacity of ω‐3 FA‐fortified meat products upon storage. © 2019 Society of Chemical Industry
Storage stability of soluble pigments, chlorophylls, and carotenoids in electron‐beam‐irradiated edible lavers (Porphyra umbilicalis) with impact on microbial safety and sensory characteristics
Authors: Lee Eun‐Jin , Ramakrishnan Sudha Rani , Kim Gui‐Ran , Kwon Joong‐Ho 
Published: January 2019

AbstractBACKGROUNDIn this study, we investigated whether electron beam (e‐beam) irradiation can inactivate microbial populations, including aerobic bacteria plate count, fungi, and coliforms, in two types of lavers (dried and roasted). The impact of e‐beam doses of 4 and 7 kGy on microbial populations, color values, total soluble pigments, chlorophylls, and carotenoid content immediately and after storage at 4, 15, 25, and 35 °C for 4, 8, and 12 weeks was also evaluated.RESULTSThe initial microbial load of approximately 106 and 105 CFU g−1 in both dried and roasted lavers was reduced to 104 and 101 CFU g−1, respectively, after irradiation at 7 kGy. No further proliferation was observed in irradiated dried lavers during 4 weeks of storage at 4 and 15 °C. When stored at low temperature, the shelf‐life was extended for up to 12 weeks in 7 kGy irradiated dried and roasted lavers. High amounts of soluble pigments and carotenoids were observed during storage in the 7 kGy irradiated dried laver samples at the end of 12 weeks irrespective of temperature. Although the chlorophyll content decreased under the storage conditions, it was comparatively stable in the 7 kGy‐irradiated dried laver. Maximum degradation of color was observed in laver samples subjected to the roasting process.CONCLUSIONOverall, this study indicated that e‐beam irradiation of laver at 7 kGy maintains microbial safety as well as quality attributes during storage for up to 12 weeks. Further, dried laver had more essential compounds than roasted laver. © 2019 Society of Chemical Industry
An in situ electrochemical fabrication of layer by layer graphenized graphite polyaniline as a stable solid‐phase microextraction fiber coating for trace environmental analysis
Authors: Razmi Habib , Manafi Khoshmanesh Sara 
Published: January 2019

AbstractA layer by layer graphenized graphite/polyaniline coating was fabricated on commercial pencil lead substrate by an in situ facile, fast and efficient electrochemical procedure. The electrodeposited polyaniline film on surface‐confined graphene structure can integrate the advantages of the both layers and was used for solid‐phase microextraction. Effective experimental parameters in electrochemical production of graphene nanosheets and electropolymerization of aniline were optimized. The prepared fiber was used for extraction and determination of four polycyclic aromatic hydrocarbons: phenanthrene, anthracene, fluoranthene and pyrene in aqueous samples by high performance liquid chromatography. The fiber coating was characterized by Fourier transform infrared spectroscopy, X‐ray diffraction and scanning electron microscopy. Under the optimized conditions, the coating provided good linear ranges (0.10–25 μg/L for phenanthrene, 0.05–12.5 μg/L for anthracene, 0.825–99 μg/L for fluoranthene and 0.625–75 μg/L for pyrene (R2 = 0.999)) and limits of detection, 0.016 to 0.275 μg/L. The produced coating has several attractive features such as high stability, low cost and long operation time.
Rapid analysis of pyridinoline and deoxypyridinoline in biological samples by liquid chromatography with mass spectrometry and a silica hydride column
Authors: Naffa Rafea , Watanabe Seiichiro , Zhang Wenkai , Maidment Catherine , Singh Preet , Chamber Paul , Matyska Maria T. , Pesek Joseph J. 
Published: January 2019

AbstractPyridinoline and deoxypyridinoline crosslinks are biomarkers found in urine for collagen degradation in bone turnover. For the first time, a rapid, sensitive, and ion‐pairing free method is described for the analysis of pyridinoline and deoxypyridinoline using ultra‐high performance liquid chromatography with Cogent Diamond Hydride column and detection by Q Exactive hybrid quadrupole‐orbitrap high resolution accurate mass spectrometry. The separation was achieved using both isocratic and gradient conditions and run time <5 min under isocratic conditions of 20% acetonitrile in water containing 0.1% formic acid. Pyridoxine was used as an internal standard and relative standard deviation of the retention times of both pyridinoline and deoxypyridinoline were <1%. The limit of detection was 0.082 ± 0.023 μM for pyridinoline and 0.118 ± 0.052 μM for deoxypyridinoline. The limit of quantitation was 0.245 ± 0.070 μM for pyridinoline and 0.354 ± 0.157 μM for deoxypyridinoline. The method was validated by the detection and quantitation of both pyridinoline and deoxypyridinoline in skin and urine samples.
A hybrid triazine‐imine core‐shell magnetic covalent organic polymer for analysis of pesticides in fruit samples by ultra high performance liquid chromatography with tandem mass spectrometry
Authors: Liang Ruiyu , Peng Yi , Hu Yuling , Li Gongke 
Published: January 2019

AbstractA novel hybrid triazine‐imine core‐shell magnetic covalent organic polymer with high adsorption capacity and excellent stability was fabricated by surface‐assisted in situ growth technique. The composite possesses porous and extended π‐conjugated system, and was applied as the magnetic sorbent for efficient enrichment and rapid separation of pesticides. A new analytical method for simultaneous determination of eight pesticides in fruit samples was developed by magnetic solid phase extraction combined with ultra high performance liquid chromatography and tandem mass spectrometry. The effect of extraction time, desorption time, and the type of desorption solvent on the extraction efficiency were evaluated. The established method shows good repeatability and high sensitivity. The repeatability of this method was estimated with relative standard deviations in the range of 0.7–7.0% (n = 5) for the same batch, and 1.7–10% (n = 3) for batch to batch. Good linearity for eight pesticides was obtained with coefficient of determination in the range of 0.9942–0.9990. Limit of detections ranged from 0.4 to 1.2 ng/L. Real sample determination showed that four and two pesticides were detected in strawberry and grape, respectively. The results demonstrated that the established method was efficient, sensitive, and convenient for trace determination of pesticides in fruit samples.
Preparation of Light‐Responsive Aliphatic Polycarbonate via Versatile Polycondensation for Controlled Degradation
Authors: Sun Jingjiang , Anderski Juliane , Picker Marie‐Theres , Langer Klaus , Kuckling Dirk 
Published: January 2019

AbstractStarting from (2,2,5‐trimethyl‐1,3‐dioxan‐5‐yl)methanamine with light‐responsive 4,5‐dimethoxy‐2‐nitrobenzyl protecting groups, a variety of light‐responsive copolycarbonates (LrPCs) are synthesized by a general two‐step polycondensation using lithium acetylacetonate (LiAcac) as catalyst. UV/Vis, 1H nuclear magnetic resonance (NMR), and size exclusion chromatography (SEC) confirm the rapid decomposition of these polymers in response to irradiation with UV light. Stable and monodisperse nanoparticles with hydrodynamic diameters of 100 nm, formulated from 25% LrPC and 75% poly(lactic‐co‐glycolic acid) (PLGA), undergo rapid disruption upon triggering with UV light, while standard PLGA nanoparticles remain stable. Moreover, differing from the ring‐opening polymerization (ROP) of trimethylene carbonate‐based monomers, direct polycondensation of 1,3‐propanediol‐based monomers with pendent functional groups and other diols will enable the introduction of various properties into the polycarbonate backbone, and expand the family of biodegradable synthetic polymers for potential biomedical applications.
Preparation of Anti‐Nonspecific Adsorption Chitosan‐Based Bovine Serum Albumin Imprinted Polymers with Outstanding Adsorption Capacity and Selective Recognition Ability Based on Magnetic Microspheres
Authors: Wang Yufei , Zhou Jingjing , Gu Huimin , Jia Xiangkun , Su Kehe , Zhang Baoliang , Zhang Qiuyu 
Published: January 2019

AbstractBovine serum albumin (BSA)‐imprinted magnetic microspheres are successfully fabricated by a precipitation copolymerization, in which 2‐hydroxyethyl acrylate (HEA) and water‐soluble chitosan separately work as functional monomers and cross‐linking agents. Hydroxyl derived from HEA as well as hydroxyl, amino, and carboxyl groups, originating from chitosan on the surface of the prepared imprinted microspheres, can form hydrogen bonds and extra electrostatic interaction with proteins. The sulfobetaine methacrylate (SBMA) is introduced into the imprinted polymer as an anti‐protein segment to reduce the nonspecific adsorption and improve the selectivity. Detailed characterization demonstrates that the imprinted layer is about 10 nm, which is extremely beneficial for BSA identification and quality transmission. Furthermore, these microspheres also display an excellent selective adsorption of BSA (IF = 5.0). The results reveal that the prepared imprinted materials can be used as BSA biosensor or separation materials.
Vascular access infection by Staphylococcus aureus from removed dialysis accesses
Authors: Chu Chishih , Wong Min Yi , Tseng Yuan‐Hsi , Lin Chun‐Liang , Tung Chun‐Wu , Kao Chih‐Chen , Huang Yao‐Kuang 
Published: January 2019

AbstractHemodialysis patients are particularly vulnerable to Staphylococcus aureus infection, with the vascular access serving as the site of entry for this formidable pathogen. Patients with arteriovenous grafts (AVGs) and tunneled‐cuffed catheters (TCCs) are at elevated risk of S. aureus infection. In this study, we investigated the correlation between the clinical characteristics of S. aureus vascular access infection (VAI), molecular profiles, and the biofilm formation abilities of clinical isolates of S. aureus. We collected samples of methicillin‐resistant S. aureus (MRSA), methicillin‐sensitive S. aureus (MSSA), and methicillin‐sensitive S. argenteus (MSSAg) from patients with S. aureus VAI and patients with other infections. The molecular profiles of the clinical isolates were determined using disk diffusion testing and molecular typing. The biofilm formation ability was determined by microtiter plate assay. In total, 63 S. aureus and 10 S. argenteus isolates were identified: 40 MRSA, 23 MSSA, and ten MSSAg. MRSA was highly prevalent (77.8%) in TCC isolates and was multidrug resistant. Of the 40 MRSA isolates, ST239‐SCCmec III was the predominant clone. SCCmec type IV was the predominant type (35%) in isolates from AVGs, while SCCmec type III was prevalent in TCC infection and showed significantly higher biofilm formation ability than types IV and V. In dialysis VAI by S. aureus, patients with TCC were more often infected with MRSA than patients with AVG, and MRSA in TCC–VAI was predominantly SCCmec type III, which had the strongest drug resistance and biofilm formation ability.
Electrophysiological determinants of the clinical severity of axonal peripheral neuropathy
Authors: Zis Panagiotis , Hadjivassiliou Marios , Rao Dasappaiah Ganesh , Sarrigiannis Ptolemaios Georgios 
Published: January 2019

AbstractIntroduction: Electrophysiological diagnosis of axonal peripheral neuropathy (PN) is based on the attenuated amplitudes of nerve conduction studies (NCS), or a reduced sural/radial amplitude ratio (SRAR). We aimed to identify the electrophysiological determinants of the clinical severity of PN. Methods: Patients with chronic axonal PN underwent detailed NCS. The clinical severity of PN was determined based upon the overall neuropathy limitations scale (ONLS). Results: Ninety‐five patients (71.6% males, mean age 71.9 ± 9.0 years) were recruited. Significant correlations were observed between the radial sensory nerve action potential (SNAP) and the ONLS total score (Spearman's rho ‐0.382, p < 0.001); and between the tibial compound muscle action potential and the ONLS leg score (Spearman's rho ‐0.283, p = 0.005). No correlations between the SRAR and the ONLS scores were found. Discussion: The radial SNAP is the strongest electrophysiological determinant of PN severity and might be useful for monitoring disease progression or response to treatment. Muscle Nerve 59:491–493, 2019
Normative data of muscle fiber diameter of vastus lateralis during childhood: a field test
Authors: Valentine Jane , Dyke Jason , Ward Rosyln , Thornton Ashleigh , Blair Eve , Stannage Katherine , Elliott Catherine , Fabian Vicki 
Published: January 2019

ABSTRACTIntroduction: Currently, our knowledge of standard data for muscle morphology in children is largely limited to the 1969 article by Brooke and Engel (BE). In 2016, we reported normal muscle morphology from vastus lateralis biopsies in ambulant children with cerebral palsy (CP). This report compares our normal biopsy results against BE standard value criteria. Methods: Single‐blind prospective cross‐sectional study design. Results: Results of biopsies taken in ambulant children with CP were normal according to morphometry and light and electron microscopy; however, only 5 of 10 fulfilled the BE standard value criteria. Discussion: This short report highlights the requirement for contemporary age‐specific normative data from a larger number of biopsies, including typically developing children. Review of the literature suggests that biopsy material may be available from typically developing children who were control patients in research trials. This morphometric data could contribute to expanding the normative data set. Muscle Nerve 59:590–590, 2019