bluuebunny/crossref_metadata_2025_split huggingface dataset:
Time-dependent cytotoxicity of dichloroacetate and metformin against Lewis lung carcinoma
Authors: Kolesnik D.L. , Pyaskovskaya O.N. , Yakshibaeva Yu.R. , Solyanik G.I. 
Published: April 2019

Summary. The use of inhibitors of energy metabolism of malignant cells is a new promising trend in the treatment of cancer patients, based on one of the unique features of the malignant cell, namely the dominance of glycolysis over oxidative phosphorylation, even in the presence of oxygen, the so-called Warburg effect. Aim: To study time-dependent cytotoxicity of sodium dichloroacetate (DCA) and metformin (MTF) against metastatic tumor cells and action of these agents on tumor cell migration. Materials and Methods: In the study low metastatic LLC/R9 variant of Lewis lung carcinoma was used. The number of living cells in the cytotoxic test was evaluated using sulforhodamine B after 1, 2 and 3 days of cell incubation in vitro. The parameters of the sensitivity of tumor cells to the action of DCA and MTF in vitro were calculated using nonlinear and linear regression of experimental data. The effect of DCA and MTF on cellular motility in vitro was evaluated using a Boyden chamber by calculation of the number of cells that migrated to the bottom side of the filter within 3 days of incubation. The statistical analysis of the data was carried out with the use of descriptive methods, Student’s t-criterion, nonlinear, and linear regression analysis. Results: IC50 of DCA was found to be equal to 50.8 ± 7.6 mM at the first day of incubation with LLC/R9 cells and decreased by 1.9 (p < 0.05) and 2.1 (p < 0.05) times at the 2nd and 3rd days, respectively. Despite the almost identical ІC50 at the 2nd and 3rd days, an increase in the incubation period of cells with DCA for up to 3 days increased the C0 parameter, which reflects the maximum concentration of the agent that does not exhibit cytotoxic effects, by 93% (p < 0.05) compared to this at the 2nd day (16.2 ± 1.4 mM vs 8.4 ± 1.0 mM, correspondently). Unlike DCA, the LLC/R9 cell population was not homogeneous by the sensitivity to the action of MTF; at least at the 3rd day, an appearance of MTF-resistant subpopulation was observed, accounting for 35% of all cells. IC50 of MTF was equal to 12.1 ± 0.6 mM, and unlike DCA, this index progressively decreased at the 2nd and 3rd days by 1.4 (p < 0.05) and 9.3 times (p < 0.05) respectively. Action of DCA at a concentration of 25 mM alone and in combination with MTF at the concentrations of 0.1 mM and 0.7 mM resulted in an increase in cell migration by 65% (p < 0.05), 63% (p < 0.05) and 78.5% (p < 0.05), respectively. There was no significant effect of MTF on the tumor cell migration. Conclusions: The sensitivity of the metastatic Lewis lung carcinoma cells to the action of the modifiers of the energy metabolism increased significantly with an increase in the incubation period, apparently, primarily due to the shortage of nutrient substrates and, in particular, glucose, indicating the relevance of their combined use as well as with other agents, which promote the deficiency of glucose in the tumor microenvironment.
DNA oxidation in patients with metastastic colorectal cancer: clinical significance of 8-hydroxy-deoxyguanosine as an independent prognostic factor
Authors: Burlaka A.A. , Vovk A.V. , Burlaka A.P. , Kolesnik O.O. 
Published: April 2019

Summary. Prognosis of metastatic colorectal cancer (mCRC) patients nowadays is an important subject in the field of oncology. R0-resection of colon with primary tumor and liver metastasis remains the only treatment which significantly improves survival rate. However, recent experimental data show that surgical trauma can indirectly stimulate tumor growth due to mitochondrial dysfunction and unregulated superoxide radical (O2-) generation. Aim: To study the clinical significance of 8-oxo-2'-deoxyguanosine (8-oxodGu) marker, to assess the oncological effects of warm ischemia of liver parenchyma on disease prognosis in patients with mCRC. Material and Methods: 69 urine 24-hour volume tests of patients with mCRC and 17 healthy individuals were studied. Urine 8-oxodGu level was measured using spectrophotometric method with pre-solid phase DNA extraction. The energy system and hepatocyte detoxification system state, levels of O2- in tumor tissue were determined using the method of electron paramagnetic resonance (EPR) and SpinTraps technology at room temperature. Experiments were carried out on a computerized EPR spectrometer RE-1307. EPR spectra were recorded at temperature of liquid nitrogen (–196 °C) in paramagnetically pure quartz dewar on a computerized spectrometer PE-1307 with resonator H011. Error of the method of spectrum integration and spread of spectrum reproduction of one sample was not more than 3%. Results: The average level of marker in healthy individuals was 0.244 ± 0.063 nM/kg ∙ day, whereas before the resection and on day 3 after the R0-resection of liver in mCRC patients was 3.42 ± 0.18 nM/kg ∙ day and 2.12 ± 0.08 nM/kg ∙ day (p < 0.05), respectively. On day 3 after the liver resection due to its metastatic lesions with a total duration of warm ischemia period < 30 min and > 30 min have had marker at level 2.108 ± 0.13 nM/kg ∙ day and 2.9883 ± 0.159 nM/kg ∙ day (p < 0.0001), respectively. The volume of metastatic tissue significantly and directly correlated with the level of urine 8-oxodGu (R2 = 0.54, 95% CI 0.037–0.0991, p < 0.0001), also duration of surgical intervention (≥ 300 min) and duration of worm liver ischemia (> 30 min) during the surgery significantly increased urine level of 8-oxodGu (R2 = 0.54, 95% CI 0.001–0.004, p < 0.001). Conclusions: Warm liver ischemia (> 30 min), long-term surgical intervention (≥ 300 min) and metastatic tissue volume (≥ 12 cm3) in liver parenchyma in mCRC patients significantly increase urine 8-oxodGu levels. R0-resection of liver metastases in mCRC patients decreases urine 8-oxodGu levels already on day 3 after the surgery. 8-oxodGu is a new factor of oncological prognosis in patients with mCRC.
Radiation research to determine local tumor invasion in patients with cervical cancer
Authors: Bakai O.A. , Golovko T.S. , Ganich A.V. , Gavriluk O.N. , Ashykhmin A.V. 
Published: April 2019

Summary. The main task of radiation study of verified cervical cancer (CC) is tumor spread assessment because of its profound effect on the treatment tactics choice and prognosis. The Aim of the study was radiation study optimizing for tumor local spread assessment in patients with CC via comparing the usefulness of different magnetic resonance imaging (MRI) based approaches. Materials and Methods: 62 patients with CC were examined by MRI on tomograph 1.5 Т using contrast enhancement and diffusion-weighted imaging (DWI). Pre-operation data on radiation diagnostics of tumor spread were compared with surgical and pathologic data. Results: The diagnostic efficiency of different methods of radiation testing for assessment of local tumor spread in patients with CC was determined. The use of contrast enhancement didn’t increase an informative value of МRI in assessment of local tumor spread. False overestimation of tumor spread was caused by intense accumulation of contrast substance around the tumor due to inflammation. Use of DWI allowed to reduce the number of false positive results, and significantly increased the efficiency of MRI (p < 0.05): the positive predictive value, sensitivity, specificity and accuracy were 83.3; 90.9; 96.0 and 95.1%, respectively. Conclusions: The use of DWI increases informativeness of МRI in assessment of local tumor spread.
Aggregated proteins in malignant and benign neoplasms
Authors: Zabolotnyi D.I. , Belousova A.A. , Zabolotna D.D. , Savchenko T.D. , Voroshylova N.M. , Timchenko M.D. , Tsvirinko I.R. , Verevka S.V. 
Published: April 2019

Summary. Aim: To evaluate the presence of the aggregated proteins in malignant and benign neoplasms for clarifying the role of impaired protein metabolism in the formation of the altered tissues. Object and Methods: The histological specimens prepared from the operative materials of 196 patients with different forms of malignant and benign neoplasms were stained with Congo red and Thioflavin T and studied under the light and polarization microscope. Results: The various forms of β-stacked protein aggregates (β-SPA) inclusions were detected in amyloids, keloid tissue, benign polyps, and several malignant tumors. Conclusion: The formation of non-functional protein aggregates proves the complex character of the impairment of protein metabolism resulting in local or systemic accumulation of secondary protein toxins results in β-SPA formation as the self-sustaining complex of parametabolic processes. The β-SPA formation is of considerable interest since their properties lead to the impairment of the normal physiological processes in adjacent tissues ensuring the chronic course of the pathology.
Expression profiling of plac1 in murine cancer cell lines
Authors: Mahmoudian J. , Ghods R. , Nazari M. , Jeddi-Tehrani M. , Ghahremani M.H. , Ostad S.N. , Zarnani A.H. 
Published: April 2019

Summary. Aim: Placenta-specific 1 (PLAC1) is among recently-discovered placental antigens which exerts fundamental role in placental function and development. Increasing body of literature shows that PLAC1 is frequently activated and expressed in a wide variety of human cancers and promote cancer progression. However, no data is available regarding the expression of mouse orthologue, plac1, in murine cancer cell lines. Materials and Methods: We investigated the expression of plac1 in a series of murine cell lines from different histological origins, mammary carcinoma (4T1), melanoma (B16F10), colorectal carcinoma (CT26), renal carcinoma (Renca), glioma (GL26), B-cell lymphoma (A20 and BCL1) and also two fibroblast cell lines (NIH3T3 and L929), using RT-PCR, Western blotting and flow cytometry. Results: Our data demonstrated that plac1 transcript and plac1 protein were expressed in all examined cell lines, as judged by RT-PCR and Western blot, respectively. The molecular weight of mouse plac1 was experimentally observed to be approximately 24 kD. Flow cytometric analysis showed surface expression of plac1 in aforesaid cell lines ranging from 2% to 42.5%. Conclusion: Based on the ubiquitous expression of plac1, the investigated cancer cell lines or immortalized cell lines can be used to examine the role of plac1 in the process of immortalization.
The open science infrastructure in Russia and the world
Authors: Zasurskiy I. I. , Trishchenko N. D. 
Published: April 2019

The article gives an overview of open access projects that form the new infrastructure for scientific communication. It provides information on initiatives and programs contributing to the development of open science in Russia and in the world. The paper also describes innovative scientific projects and platforms that significantly accelerate exchange of information and communication between scientists and create new opportunities for their cooperation and for society.The scientific novelty of the article consists in the distinction between the notions of ‘publication’ and ‘article’ or ‘edition’ in the context of scientific communication and the work of libraries, the description of the challenges that the new media era puts behind the system of scientific communications and libraries, and Russian projects, which aim to solve the described problems.The system of scientific communication has transformed significantly over the past two decades, alternative channels of information exchange are beginning to play a leading role and become the competitors of the traditional media (scientific journals), which have converted into electronic form, but still remain rooted in the past, supported by the inertia of administration of science.In Russia the achievements of open science are still much too modest compared to Europe, but the necessary background has already been created by libraries and non-for-profit organizations. Further development of such projects will depend on whether government, libraries and universities could use these instruments and the capabilities of the new media, reorienting to a new paradigm of access to scientific information.
El nivel del compromiso organizacional de los funcionarios públicos en la mejora de la gestión educativa y los objetivos estratégicos de la coordinación zonal 5 de educación - caso Ecuador.
Authors: Pacheco Mendoza Eythel Blanmer , Cedillo Fajardo Miguel Angel 
Published: April 2019

El presente artículo hace referencia a la importancia que existe en el nivel del compromiso organizacional de los funcionarios públicos de la Coordinación Zonal 5 de Educación, y que se van a ver afectados directamente en la mejora de la gestión educativa, que busca de una manera lograr alcanzar a cumplir los objetivos estratégicos de esta institución.
Es importante indicar que en la gestión tiene una vinculación directa con el compromiso organizacional, que busca de una u otra forma la satisfacción plena de los clientes ya sea, internos o externos, con esta investigación buscamos contribuir a la mejora en la administración pública, empoderar a los funcionarios y hacer de esta forma, que las gestiones mejoren en el aspecto administrativo de Ganar-Ganar con el único objetivo que es el de mejorar y cumplir con lo que el Estado requiere en sus instituciones públicas, así como la existencia de un clima organizacional óptimo se puede determinar que se cumplirán los objetivos estratégicos en el campo educativo.
RANCANG BANGUN SISTEM PEMESANAN CATERING DAN DEKORASI DI SUMBAWA BESAR BERBASIS ANDROID
Authors: Rodianto Rodianto , Safitri Arni 
Published: April 2019

The catering and decorating process in Sumbawa is still conventional. So that the service procedure which applied for now are still less effective and efficient. This process incude to the owners of catering and decoration that could does a mistake, among others, the double order, the order making was not in order due to the accumulation of order notes, especially when crowded customers ordered by telephone or short message which could occur human error such as forgetting the order customer, and pen or order paper that runs out. The software is built by using the programming languages PHP, MySQL, Java and Android Studio. The purpose of making this ordering system is to help the owner of catering nusiness and decoration in Sumbawa besar to improving the quality of their business in the eyes of customers. This ordering system has a service that makes customers know the that location of the place of residence of the business owner as to minimize the occurrence of customer concers for message that are not served. The method used is a method of data collection and software development. The software development process model is used in the waterfall model which consist (1) analysis, (2) design, (3) coding, (4) program testing, (5) maintance.
Klasifikasi Wajah Menggunakan Support Vector Machine (SVM)
Authors: Rizal Reyhan Achmad , Girsang Imron Sanjaya , Prasetiyo Sidik Apriyadi 
Published: April 2019

Klasifikasi wajah merupakan teknik yang dapat digunakan untuk membedakan karakteristik pola wajah seseorang. Sistem klasifikasi wajah adalah suatu aplikasi yang membuat sebuah mesin dapat mengenali wajah seseorang sesuai dengan citra wajah yang telah ditraining dan disimpan di dalam database mesin tersebut. Klasifikasi wajah sendiri dapat dilakukan dengan berbagai cara, salah satunya adalah menggunakan metode support vector machine (SVM). Penelitian ini dilakukan dengan sampling yang di ambil dalam variasi posisi pada sudut kemiringan subjek (-90°, -70°, -45°, -25°, -5° ) dan (+90°, +70°, +45°, +25°, +5° ) dengan ukuran citra 640x480. Sistem klasifikasi wajah didalam penelitian ini dibangun dengan menggunakan metode support vector machine (SVM) dan bahasa pemograman Matlap. Penelitian ini menghasilkan tingkat true detection 90% dan false detection 10% dari jumlah sampel 200 subjek yang digunakan.
Keywords— Klasifikasi wajah, sudut kemiringan, SVM
ANALISA TERHADAP PUTUSAN MAHKAMAH KONTITUSI NOMOR 46/PUU-VIII/2010 MENGENAI STATUS ANAK
Authors: Pasmatuti Darda 
Published: April 2019

Marriage is a sacred part of life, because it must pay attention to norms and rules of life in society. But in reality, not everyone is so principled, with a variety of justifiable reasons that are reasonable and acceptable to society, marriage is often not appreciated for its sanctity. Marriage is a medium that will unite two people in a household. Marriage is the only unifying rite of two persons officially recognized in both state law and religious law. Problem under study is to analyze the decision of Constitutional Court Number 46/PUU-VIII/2010 regarding the status of children, in this case the research method used is descriptive research that emphasizes the data in the form of narrative and argumentation of words and not on data in the form of numbers, numbers. Using the normative juridical approach of trying to learn every legal material that is related to the subject of the study and then comparing with the rules of legislation, theories and opinions of experts on the issues discussed. Test material submitted from Hj. Aisha Mochtar to the Constitutional Court regarding the provisions of Article 2 and Article 43 paragraph (1) of Law no. 1 of 1974 concerning Marriage of Article 28 B Paragraph (1) and (2) of the 1945 Constitution relating to the Rights of the Child and Article 28 D of the 1945 Constitution relating to the right of everyone to the recognition, guarantee, protection and certainty of equitable law and equal treatment in before the law. Article 2 of Law no. 1 Year 1974, related to legitimated marriage is marriage recorded at the institution of marriage. Meanwhile, Article 43 of Law no. 1 Year 1974, related to the status of children outside marriage. The test of the material is granted in decision No. 46/PUU-VIII/2010. Which will be discussed is the Decision Analysis of the Constitutional Court. Decision 46/PUU-VIII/2010 child status.
Diferencia de género en la profesión de contador público en Panamá: Un problema ético
Authors: Chanmarín Reyes Carlos Antonio 
Published: April 2019

El objetivo general de esta investigación fue determinar si el hombre y la mujer se pueden desempeñar en igualdad de condiciones en la profesión de contador público en Panamá, en el año 2015. Los objetivos específicos tienen que ver con las variables: preparados, reciben, familia, crediticias, discriminación, prejuicio, estrictos, experiencia y ético. Se aplicó, a los datos recibidos de 240 encuestados, los análisis Alfa de Cronbach, Pearson y Espearman, tabulación simple y resultó que la condición de la mujer no es igual que la del hombre, que ambos están preparados para ejercer la profesión de contador público, la mujer no recibe el mismo salario, ni facilidades crediticias, ni condición de familia, que son más estrictas que el hombre, que es importante la experiencia y que sí es un problema ético.

Palabras clave: economía de género, discriminación laboral

ABSTRACT

The general objective of this research was to: Determine if men and women can play under equal conditions in the profession of public accountant in Panama, in 2015. The specific objectives have to do with the variables: prepared, receive, family, credit, discrimination, prejudice, strict, experience and ethical. It applied, to the data received from 240 respondents, the Alpha analyzes of Cronbach, Pearson and Espearman, Simple Tabulation and it turned out that the condition of the woman is not the same as that of the man, that both are prepared to practice the profession of public accountant , women do not receive the same salary, credit facilities, or family status, which are stricter than men, which is important experience and which is an ethical problem.

Key words: gender economics, employment discrimination
La carga física de los trabajadores: estrategia administrativa en la mejora de procesos
Authors: Real Pérez Grether Lucía , Hidalgo Ávila Argelio Antonio , Ramos Alfonso Yanelis 
Published: April 2019

La administración surge cuando existe la necesidad de manejar recursos. Los administradores deben planificar, organizar, dirigir y controlar todos los elementos de los procesos, considerando los medios de trabajo, los objetos de trabajo y las personas. Este último es el eslabón fundamental del proceso productivo o de servicio. Se debe considerar además el entorno y su relación con el medio ambiente, permitiendo ser a las empresas responsables con los tres pilares fundamentales: la sociedad, el medio ambiente, y la economía. La Ergonomía, como ciencia aplicada, permite garantizar las condiciones de seguridad, salud y bienestar a sus trabajadores, logrando ser más productivos, eficientes, eficaces y competitivos. El objetivo de la investigación fue conocer las implicaciones de la carga física en los trabajadores, utilizando una herramienta de diagnóstico que permitió, como resultado fundamental, el establecimiento de programas de mejoras ajustados a cada una de las realidades de los puestos de trabajo.
Palabras clave: Ergonomía, Administración, Carga física, Factores de riesgo, Programa de mejoras
Interdisciplinariedad curricular contable en el contexto profesional actual en las universidades colombianas
Authors: Patiño Jacinto Ruth Alejandra , Valero Zapata Gloria Milena , Díaz Jiménez Michael Andrés 
Published: April 2019

El presente trabajo tiene como propósito analizar la incorporación de contenidos interdisciplinares en el currículo profesional contable, más allá de los criterios normativos establecidos como condiciones mínimas para los Programas de Formación Profesional de pregrado en Contaduría Pública (Resolución 3459, 2003); teniendo en cuenta los nuevos requerimientos en el contexto global y nacional, particularmente los relacionados con el proceso de convergencia hacia estándares internacionales de contabilidad y estándares internacionales de información financiera, que se viene desarrollando en Colombia mediante la promulgación de la Ley 1314 de 2009. El estudio descriptivo propuesto se desarrolló, a partir de una metodología cuantitativa, que busca analizar los planes de estudio contables de una muestra de 77 universidades colombianas; con el fin de identificar las características generales de las asignaturas complementarias a la formación básica del contador y su contribución al desempeño profesional, como a la denominada “formación integral” que se promulga frente a los retos del mundo actual.
Palabras clave: Currículo, formación profesional contable, formación interdisciplinar, plan de estudios, formación integral.
Selección de indicadores de gestión y desarrollo territorial en municipalidades
Authors: Cobeña Andrade Xavier Alfredo 
Published: April 2019

La presente investigación tiene como objetivo que los planificadores del desarrollo municipal, autoridades locales y sociedad civil, conozcan parámetros de medición de los resultados de la gestión pública, de los esfuerzos de la sociedad civil y las inversiones privadas en el progreso de sus comunidades. Esto se logra a través del análisis de experiencias previas en la formulación de planes de desarrollo, ordenamiento territorial, desarrollo sostenible crecimiento económico, conservación ambiental, cobertura y calidad de los servicios públicos. Es necesario también, incorporar el análisis del desarrollo territorial desde una mirada multidimensional, incorporar el componente comunicacional y empoderar a la sociedad civil en el seguimiento y cumplimiento de metas propuestas. Es importante adelantar que el alcance de esta investigación es municipal, cantonal o departamental dependiendo de la división política administrativa que corresponda a cada país y tiene un sesgo cultural latinoamericano, considerando el potencial que han demostrado los municipios de esta región en realizar importantes progresos en materia de desarrollo local.
Palabras clave: Indicadores municipales, desarrollo territorial.