bluuebunny/crossref_metadata_2025_split huggingface dataset:
Epithelial sealing effectiveness against titanium or zirconia implants surface
Authors: Atsuta Ikiru , Ayukawa Yasunori , Furuhashi Akihiro , Narimatsu Ikue , Kondo Ryosuke , Oshiro Wakana , Koyano Kiyoshi 
Published: February 2019

AbstractThe aims of implant treatment now involve not only restoration of mastication function, but also recovery of esthetics. Currently, zirconia is widely used as an esthetic material for implant abutment. Therefore, it is very important to understand the efficacy of zirconia for epithelial sealing as an implant material. We compared the effects of materials on the sealing of the peri‐implant epithelium (PIE) to titanium (Ti) or zirconia (Zr) implants, for application to clinical work. Maxillary first molars were extracted from rats and replaced with Ti or Zr implants. The sealing of the PIE to the implants was evaluated with immunohistochemistry observation and HRP analysis. The morphological and functional changes in rat oral epithelial cells (OECs) cultured on Ti or Zr plates were also evaluated. After 4 weeks, the PIE on the Ti and Zr implants showed similar structures. The Zr implants appeared to form a weak epithelial seal at the tissue–implant interface, and exhibited markedly less adhesive structures than the Ti implants under electron microscopic observation. In the in vitro experiments, decreased expression levels of adhesion proteins were observed in OECs cultured on Zr plates compared with those cultured on Ti plates. In addition, the cell adherence on Zr plates was reduced, while the cell migration was low on Ti plates. Zr is a better choice for an esthetic implant material, but needs further improvement for integration with the epithelial wound healing process around a dental implant. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2019.
Does a history of childhood abuse moderate the association between symptoms of posttraumatic stress disorder and borderline personality disorder in survivors of intimate partner violence?
Authors: Beck J. Gayle , Woodward Matthew J. , Pickover Alison M. , Lipinski Alexandra J. , Dodson Thomas S. , Tran Han N. 
Published: February 2019

AbstractObjectiveThis study examined whether a history of childhood abuse (CA) strengthened the association between borderline personality disorder (BPD) and posttraumatic stress disorder (PTSD) in the aftermath of intimate partner violence (IPV). This hypothesis arises from clinical literature but has not been examined empirically. We predicted that a history of CA would enhance associations between BPD features and PTSD symptoms.MethodDimensional assessment of both PTSD and BPD was made in a sample of 211 women who sought mental health services following IPV. Two analyses were conducted using clinician‐assessed DSM‐IV (Diagnostic and Statistical Manual of Mental Disorders, 4th ed.) PTSD symptoms: (a) total score and (b) symptom clusters.ResultsUsing path analysis, results indicated significant associations between BPD features and PTSD symptoms, but no significant interaction between BPD and CA in either analysis.ConclusionsResults are discussed given current understanding of comorbidities involving PTSD, with particular attention to potential implications for clinical practice. Areas for future research are proposed.
Peptidomic analysis reveals multiple protection of human breast milk on infants during different stages
Authors: Zhou Yahui , Zhang Le , Yu Zhangbin , Zhang Aiqing , Wu Weimin , Chen Wenjuan , Yan Xiangyun , Liu Heng , Hu Yin , Jiang Chengyao , Xu Yan , Wang Xingyun , Han Shuping 
Published: February 2019

AbstractIt has been shown that human breast milk (HBM) is an important nutrient for the growth and development of newborns. Currently, peptide drugs provide promising regimes in neonatal disease treatment, especially peptides from HBM that exhibit multiple functions within cells. To explore the potential biological function peptides among the colostrum, transition and mature milk from mother of extremely low birth weight children (the samples were collected from Women's Hospital of Nanjing Medical University from December 2016 to February 2017). A total of 3,182 nonredundant peptides were identified and compared among colostrum, transitional and mature milk using liquid chromatography/mass spectrometry technology, and the numbers and fragments of peptides were various. The isoelectric point and molecular weight analysis of the differentially expressed peptides basically accord with the range of mass spectrometry identification (<3 kDa). Gene Ontology analysis and Pathway analysis, restriction sites analysis, as well as bioinformatics analysis showed that these differentially expressed peptides enriched a variety of biological processes. We identified several putative peptides that might have bioactive effects in diseases and development of newborns, which will inform further functional investigations. Our preliminary research provided a better understanding of the function of peptides during the newborn periods. Furthermore, it laid a foundation for discovering new peptide drugs in neonatal disease treatment.
Suppression of MALAT1 ameliorates chronic constriction injury‐induced neuropathic pain in rats via modulating miR‐206 and ZEB2
Authors: Chen Zhao‐Ling , Liu Jing‐Yi , Wang Fang , Jing Xu 
Published: February 2019

AbstractLong noncoding RNAs (lncRNAs) are involved in multiple nervous system diseases, including neuropathic pain. Previous studies have demonstrated that lncRNA metastasis‐associated lung adenocarcinoma transcript 1 (MALAT1) has been identified as a diagnostic biomarker in many diseases. Nevertheless, the function of MALAT1 in neuropathic pain progression is still unclear. Here, we established a chronic constriction injury (CCI) rat model. We found that MALAT1 was remarkably upregulated in CCI rats. In addition, neuropathic pain behaviors such as mechanical and thermal hyperalgesia were reduced by the inhibition of MALAT1. Meanwhile, the loss of MALAT1 was able to depress the neuroinflammation process via the inhibition of COX‐2, interleukin‐1β, and interleukin‐6. A previous study has indicated that miR‐206 upregulation can restrain the CCI‐induced neuropathic pain. Furthermore, we exhibited that miR‐206 was significantly downregulated and silence of MALAT1 restrained its expression in CCI rats. For another, ZEB2 was a target of miR‐206 and it was shown that ZEB2 was elevated in CCI rats in a time‐dependent manner. Overexpression of miR‐206 obviously suppressed ZEB2 levels in rat microglial cells. Subsequently, it was demonstrated that upregulation of miR‐206 rescued the neuropathic pain triggered by ZEB2 overexpression in vivo through neuroinflammation inhibition. Overall, we indicated that suppression of MALAT1 ameliorated neuropathic pain progression via miR‐206/ZEB2 axis.
A functional variant in the flanking region of pri‐let‐7f contributes to colorectal cancer risk in a Chinese population
Authors: Yuan Fang , Xiao Xiao , Che Guanglu , Wang Yanyun , Wang Tao , Luo Xiaolei , Liang Weibo , Gao Linbo , Zhang Lin 
Published: February 2019

AbstractLet‐7f was reported to be downregulated in patients with colorectal cancer (CRC). However, little is known about the role of let‐7f in CRC carcinogenesis. The aim of this study was to investigate the correlation between genetic polymorphisms in the flanking region of pri‐let‐7f and CRC risk, as well as the potential role of let‐7f in CRC cell migration and invasion. The pri‐let‐7f‐1 rs10739971 and pri‐let‐7f‐2 rs17276588 were genotyped using TaqMan (Applied Biosystems, Foster City, CA) assay. The luciferase activity was detected using Dual‐Luciferase Reporter Assay. CRC cell migration and invasion were evaluated using transwell chamber assay. The rs17276588 AG and AG/AA genotypes had a significantly increased CRC risk (AG vs. GG: adjusted odds ratio [OR] = 1.48, 95% confidence interval [CI] = 1.19–1.83, p < 0.001; AG/AA vs. GG: adjusted OR = 1.43, 95% CI = 1.17–1.75, p < 0.001). Stratification analyses showed that the increased risk was observed in CRC patients with well‐moderately differential status, patients with clinical Stages I–II, and patients without lymph node metastasis. The rs17276588A allele displayed a decreased transcriptional activity and low levels of let‐7f. Moreover, let‐7f inhibited migration and invasion in Caco‐2 and Lovo cells. These findings indicate that the rs17276588 AG/AA genotypes increased CRC risk by reducing the expression of tumor suppressor let‐7f.
LINC00628 suppresses migration and invasion of hepatocellular carcinoma by its conserved region interacting with the promoter of VEGFA
Authors: Chen Qiuxu , Wang Dan , Li Yongguo , Yan Shaoying , Dang Hao , Yue Huan , Ling Jiaji , Chen Fengjiao , Zhao Yannan , Gou Luxia , Tang Ping , Huang Ailong , Tang Hua 
Published: February 2019

AbstractAccumulated evidence revealed that numerous long noncoding RNAs (lncRNAs) have been found to be involved in the development and progression of hepatocellular carcinoma (HCC). LINC00628, a member of lncRNAs, has been reported to act as a tumor suppressor in gastric cancer and breast cancer. However, its potential role in HCC still remains unknown. Herein, we characterized the function of LINC00628 in HCC. Our investigation has revealed that LINC00628 were dramatically decreased in HCC tissues and cells, and inhibited the migration and invasion of HCC cells in vitro and in vivo. Moreover, LINC00628 exerted its tumor suppressive function by repressing the vascular endothelial growth factor A (VEGFA) promoter activity. A highly conserved region element in LINC00628 was identified by a cross‐species comparative analysis, which is required for LINC00628 exerted its function. Dual‐luciferase reporter assay showed that the conserved sequence mediated the interaction with a specific region of VEGFA promoter, resulting in a decrease of VEGFA expression. In conclusion, our results demonstrated that LINC00628 could function as a tumor suppressor in HCC via its conserved sequence elements interacting with a particular region of VEGFA promoter, suggesting that LINC00628 may serve as a novel promising target for diagnosis and therapy in HCC.
A novel LncRNA‐based prognostic score reveals TP53‐dependent subtype of lung adenocarcinoma with poor survival
Authors: Kumar Pranjal , Khadirnaikar Seema , Shukla Sudhanshu Kumar 
Published: February 2019

AbstractThe prognostic signatures play an essential role in the era of personalised therapy for cancer patients including lung adenocarcinoma (LUAD). Long noncoding RNA (LncRNA), a relatively novel class of RNA, has shown to play a crucial role in all the areas of cancer biology. Here, we developed and validated a robust LncRNA‐based prognostic signature for LUAD patients using three different cohorts. In the discovery cohort, four LncRNAs were identified with 10% false discovery rate and a hazard ratio of >10 using univariate Cox regression analysis. A risk score, generated from the four LncRNAs’ expression, was found to be a significant predictor of survival in the discovery and validation cohort (p = 9.97 × 10
−8 and 1.41 × 10
−3, respectively). Further optimisation of four LncRNAs signature in the validation cohort, generated a three LncRNAs prognostic score (LPS), which was found to be an independent predictor of survival in both the cohorts (
p = 1.00 × 10
−6 and 7.27 × 10
−4, respectively). The LPS also significantly divided survival in clinically important subsets, including Stage I (
p = 9.00 × 10
−4 and 4.40 × 10
−2, respectively), KRAS wild‐type (WT), KRAS mutant (
p = 4.00 × 10
−3 and 4.30 × 10
−2, respectively) and EGFR WT (
p = 2.00 × 10
−4). In multivariate analysis LPS outperformed, eight previous prognosticators. Further, individual members of LPS showed a significant correlation with survival in microarray data sets. Mutation analysis showed that high‐LPS patients have a higher mutation rate and inactivation of the TP53 pathway. In summary, we identified and validated a novel LncRNA signature LPS for LUAD.
Antitumor effects of curcumin: A lipid perspective
Authors: Naeini Mehri Bemani , Momtazi Amir Abbas , Jaafari Mahmoud Reza , Johnston Thomas P. , Barreto George , Banach Maciej , Sahebkar Amirhossein 
Published: February 2019

AbstractLipid metabolism plays an important role in cancer development due to the necessities of rapidly dividing cells to increase structural, energetic, and biosynthetic demands for cell proliferation. Basically, obesity, type 2 diabetes, and other related diseases, and cancer are associated with a common hyperactivated “lipogenic state.” Recent evidence suggests that metabolic reprogramming and overproduction of enzymes involved in the synthesis of fatty acids are the new hallmarks of cancer, which occur in an early phase of tumorigenesis. As the first evidence to confirm dysregulated lipid metabolism in cancer cells, the overexpression of fatty acid synthase (FAS) was observed in breast cancer patients and demonstrated the role of FAS in cancer. Other enzymes of fatty acid synthesis have recently been found to be dysregulated in cancer, including ATP‐dependent citrate lyase and acetyl‐CoA carboxylase, which further underscores the connection of these metabolic pathways with cancer cell survival and proliferation. The degree of overexpression of lipogenic enzymes and elevated lipid utilization in tumors is closely associated with cancer progression. The question that arises is whether the progression of cancer can be suppressed, or at least decelerated, by modulating gene expression related to fatty acid metabolism. Curcumin, due to its effects on the regulation of lipogenic enzymes, might be able to suppress, or even cause regression of tumor growth. This review discusses recent evidence concerning the important role of lipogenic enzymes in the metabolism of cancer cells and whether the inhibitory effects of curcumin on lipogenic enzymes is therapeutically efficacious.
Current approaches in identification and isolation of cancer stem cells
Authors: Akbarzadeh Maryam , Maroufi Nazila Fathi , Tazehkand Abbas Pirpour , Akbarzadeh Moloud , Bastani Sepideh , Safdari Reza , Farzane Ali , Fattahi Amir , Nejabati Hamid Reza , Nouri Mohammad , Samadi Nasser 
Published: February 2019

AbstractCancer stem cells (CSCs) are tumor cells with initiating ability, self‐renewal potential, and intrinsic resistance to conventional therapeutics. Efficient isolation and characterization of CSCs pave the way for more comprehensive knowledge about tumorigenesis, heterogeneity, and chemoresistance. Also a better understanding of CSCs will lead to novel era of both basic and clinical cancer research, reclassiﬁcation of human tumors, and development of innovative therapeutic strategies. Finding novel diagnostic and effective therapeutic strategies also enhance the success of treatment in cancer patients. There are various methods based on the characteristics of the CSCs to detect and isolate these cells, some of which have recently developed. This review summarized current techniques for effective isolation and characterization of CSCs with a focus on advantages and limitations of each method with clinical applications.
Defining early hematopoietic‐fated primitive streak specification of human pluripotent stem cells by the orchestrated balance of Wnt, activin, and BMP signaling
Authors: Shen Jun , Lyu Cuicui , Zhu Yaoyao , Feng Zicen , Zhang Shuo , Hoyle Dixie L. , Ji Guangzhen , Brodsky Robert A. , Cheng Tao , Wang Zack Z. 
Published: February 2019

AbstractDistinct regions of the primitive streak (PS) have diverse potential to differentiate into several tissues, including the hematopoietic lineage originated from the posterior region of PS. Although various signaling pathways have been identified to promote the development of PS and its mesoderm derivatives, there is a large gap in our understanding of signaling pathways that regulate the hematopoietic fate of PS. Here, we defined the roles of Wnt, activin, and bone morphogenetic protein (BMP) signaling pathways in generating hematopoietic‐fated PS from human pluripotent stem cells (hPSCs). We found that the synergistic balance of these signaling pathways was crucial for controlling the PS fate determination towards hematopoietic lineage via mesodermal progenitors. Although the induction of PS depends largely on the Wnt and activin signaling, the PS generated without BMP4 lacks the hematopoietic potential, indicating that the BMP signaling is necessary for the PS to acquire hematopoietic property. Appropriate levels of Wnt signaling is crucial for the development of PS and its specification to the hematopoietic lineage. Although the development of PS is less sensitive to activin or BMP signaling, the fate of PS to mesoderm progenitors and subsequent hematopoietic lineage is determined by appropriate levels of activin or BMP signaling. Collectively, our study demonstrates that Wnt, activin, and BMP signaling pathways play cooperative and distinct roles in regulating the fate determination of PS for hematopoietic development. Our understanding of the regulatory networks of hematopoietic‐fated PS would provide important insights into early hematopoietic patterning and possible guidance for generating functional hematopoietic cells from hPSCs in vitro.
A systematic review of radiation‐induced testicular toxicities following radiotherapy for prostate cancer
Authors: Farhood Bagher , Mortezaee Keywan , Haghi‐Aminjan Hamed , Khanlarkhani Neda , Salehi Ensieh , Nashtaei Maryam Shabani , Najafi Masoud , Sahebkar Amirhossein 
Published: February 2019

AbstractBackgroundProstate cancer is the second most common malignancy in men in the world, and radiotherapy is used as a standard treatment modality for this cancer. Although this treatment modality effectively kills prostate cancerous cells, it unavoidably irradiates the organs/tissues that are away from the treatment site. In this regard, radiation‐induced testicular toxicities following prostate radiotherapy can affect sexual function, reproduction, and quality of life in cancer survivors. This review summarizes the available data on testicular exposure to radiation during prostate radiotherapy and the consequences on testicular function.MethodsTo illuminate the radiation‐induced testicular toxicities following prostate radiotherapy, a systematic search was conducted based on the Preferred Reporting Items for Systematic Reviews and Meta‐Analyses guideline in PubMed, Web of Science, Scopus, Embase, and clinical trials electronic databases up to September 2018. According to a set of prespecified inclusion and exclusion criteria, 31 eligible articles providing data on testicular function following radiotherapy in patients with prostate cancer were included in the study.ResultsAccording to the different radiotherapeutic techniques used for prostate cancer treatment, the total tumor dose and scattered testicular dose values were ranging from 36.25 to 78.00 Gy and 0.06 to 6.48 Gy, respectively. Luteinizing hormone and follicle‐stimulating hormone levels after prostate radiotherapy were signiﬁcantly higher in comparison with the pretreatment levels. Around 60% of the studies showed that testosterone levels after prostate radiotherapy were signiﬁcantly lower than the pretreatment levels. Furthermore, erectile dysfunction (ED), as an adverse side effect resulting from prostate radiotherapy, was reported and this complication is signiﬁcantly correlated with lower satisfaction with sexual life. Testicular atrophy following prostate radiotherapy has also been observed and its frequency in patients with prior prostate radiotherapy is 2.5 times more than that in the patients without prior radiotherapy.ConclusionThe data revealed that the scattered dose to testicular tissues during prostate radiotherapy can lead to testicular atrophy, variation of the male sex hormones, and quality of sexual life.
Population Pharmacokinetics of Vamorolone (VBP15) in Healthy Men and Boys With Duchenne Muscular Dystrophy
Authors: Mavroudis Panteleimon D. , van den Anker John , Conklin Laurie S. , Damsker Jesse M. , Hoffman Eric P. , Nagaraju Kanneboyina , Clemens Paula R. , Jusko William J. 
Published: February 2019

AbstractDuchenne muscular dystrophy (DMD) is an inherited neuromuscular disorder occurring in boys and caused by mutations in the dystrophin gene. Vamorolone is a first‐generation delta‐9,11 compound that has favorable efficacy and side effect profiles relative to classical glucocorticoids. The pharmacokinetics (PK) of oral vamorolone were assessed in parallel‐group studies in healthy men (phase 1, n = 86) and boys with DMD (phase 2a, n = 48) during 14 days of once‐daily dosing with a range of doses. Vamorolone exhibited moderate variability in PK, with the maximum plasma concentration usually occurring at 2‐4 hours and a half‐life of approximately 2 hours for all doses and days examined. Population PK modeling of all data together indicated that the PK of vamorolone can be well described by a 1‐compartment model with zero‐order absorption. Both men and boys showed a dose‐linearity of PK parameters for the doses examined, with no accumulation of the drug during daily dosing. Ingestion with food resulted in markedly enhanced absorption of the drug, as tested in healthy men. There were similar PK of vamorolone in healthy men and DMD boys with apparent clearance averaging 2.0 L/h/kg in men and 1.7 L/h/kg in boys. Overall, vamorolone exhibited well‐behaved linear PK, with similar profiles in healthy men and boys with DMD, moderate variability in PK parameters, and absorption and disposition profiles similar to those of classical glucocorticoids.
Induced subgraphs of graphs with large chromatic number. XII. Distant stars
Authors: Chudnovsky Maria , Scott Alex , Seymour Paul 
Published: February 2019

AbstractThe Gyárfás‐Sumner conjecture asserts that if is a tree then every graph with bounded clique number and very large chromatic number contains as an induced subgraph. This is still open, although it has been proved for a few simple families of trees, including trees of radius two, some special trees of radius three, and subdivided stars. These trees all have the property that their vertices of degree more than two are clustered quite closely together. In this paper, we prove the conjecture for two families of trees which do not have this restriction. As special cases, these families contain all double‐ended brooms and two‐legged caterpillars.
Keeping an eye on congenital disorders of O‐glycosylation: A systematic literature review
Authors: Francisco Rita , Pascoal Carlota , Marques‐da‐Silva Dorinda , Morava Eva , Gole Glen A. , Coman David , Jaeken Jaak , dos Reis Ferreira Vanessa 
Published: February 2019

AbstractCongenital disorders of glycosylation (CDG) are a rapidly growing family comprising >100 genetic diseases. Some 25 CDG are pure O‐glycosylation defects. Even among this CDG subgroup, phenotypic diversity is broad, ranging from mild to severe poly‐organ/system dysfunction. Ophthalmic manifestations are present in 60% of these CDG. The ophthalmic manifestations in N‐glycosylation‐deficient patients have been described elsewhere. The present review documents the spectrum and incidence of eye disorders in patients with pure O‐glycosylation defects with the aim of assisting diagnosis and management and promoting research.