bluuebunny/crossref_metadata_2025_split huggingface dataset:
La pandemia de gripe de 1918-1919 en territorio asturiano: de padecimiento histórico a enfermedad emergente cien años después / The Influenza pandemics of 1918-1919 in Asturian territory: Analysis of a 100-year process going from historical suffering to emerging disease
Authors: Sánchez Fernández Luis Vicente , Arcos González Pedro , Neri Vela Rolando , Hernández Martín Elisa 
Published: June 2019

La pandemia de gripe de 1918 y 1919 ha sido considerada una de las mayores catástrofes sanitarias del siglo xx. España alcanzó una tasa de mortalidad de 13,03 por mil habitantes. Este estudio pone de manifiesto una realidad diferenciadora en el Principado de Asturias, por un lado: Oviedo, Gijón y Avilés como núcleos urbanos con una tasa de mortalidad de 8,84 por mil habitantes y de otro Cangas del Narcea representativode una zona rural donde la enfermedad duró más tiempo y causó una mortalidad significativamente mayor. Esta gripe es una enfermedad que sigue activa entre nosotros.La pandémie de grippe de 1918 et 1919 en Espagne a étéconsidérée comme l’une des plus grandes catastrophes sanitaires du xxe siècle, atteignant un taux de mortalité de 13,03 pour mille habitants. Cette étude montre de différents impacts de l’épidémie dans les zones urbaines et rurales de la Principauté des Asturies. Dans les zones urbaines d’Oviedo, de Gijón et d’Avilés, le taux de mortalité était de 8,84 pour mille habitants, tandis que dans la zone rurale de Cangas del Narcea, la maladie a duré plus longtemps et a entraîné une mortaliténettement plus élevée. Bien que présentant des différences épidémiologiques et temporelles, la grippe reste une maladie périodiquement présente parmi nous.The 1918/19 influenza pandemic has been considered one of the biggest health disaters of the twentieth century. In Spain it reached a mortality rate of 13.03 per thousand inhabitants. This study shows a distinct reality in the Principality of Asturias, on the one hand we have Oviedo, Gijón and Avilés as centers of urban structure with a mortality rate of 8.80 per thousand inhabitants. On the other hand, Cangas del Narcea, representing a rural area, where the disease lasted longer and caused a significantly higher mortality. Flu is a disease that is still active today.
Psoriazisin klinik şiddeti ile histopatolojik bulgular arasında ilişki var mıdır? klinikopatolojik bir çalışma
Authors: ASLAN Figen 
Published: June 2019

Amaç: Psoriazis, şiddeti hastadan hastaya değişen yaygın bir kronik inflamatuar deri hastalığıdır. Bu çalışmada, histopatolojik bulgular ile hastalığın klinik şiddeti arasında ilişki olup olmadığını araştırmayı amaçladık.Gereç ve Yöntem: Bu retrospektif çalışmada, hem klinik hem de deri biyopsisi ile histopatolojik olarak psoriazis vulgaris tanısı konulan 51 olgu incelendi. Olguların parafin bloklarından hazırlanan kesitleri mikroskobik olarak incelendi ve bulgular çeşitli histopatolojik özellikler açısından kaydedildi. Hastalığın şiddetini değerlendirmek için psoriazis alan şiddet indeksi (PASI) kullanıldı. PASI ile ilgili veriler hastaların tıbbi dosyalarından elde edildi. Hastalar daha sonra yüksek PASI skoru (≥10) ve düşük PASI skoru (&lt;10) olanlar olarak gruplandırıldı. Histopatolojik özellikler ile PASI skoru arasındaki ilişki Pearson korelasyon testi ile değerlendirildi.Bulgular: Elli bir hastanın yaş ortalaması 45.19 idi. Çalışmada, Psoriazis vulgaris olgularında (31 erkek, 20 kadın) 1.5: 1 olan erkek: kadın oranıyla erkek baskınlığı görüldü. Psoriazisin aile öyküsü % 29 hastada pozitifti. En sık görülen histopatolojik özellikler: hiperkeratoz (% 100), parakeratoz (% 100) (fokal parakeratoz (% 23), konfluent parakeratoz (% 77)), rete ridgelerin uzaması (% 98) ve vasküler dilatasyon (% 88) idi. Hastaların 30'unda (% 58.8) düşük PASI skoru (&lt;10), 21'inde (% 41.2) yüksek PASI skoru (≥10) vardı. Hastalığın şiddeti (PASI skoru) ile histopatolojik özelliklerin herhangi biri ile ilişki bulunmadı. Sonuç: Psoriazisin karakteristik temel histopatolojik bulguları olmasına rağmen bu histopatolojik bulguların hiçbiri doğrudan hastalığın klinik şiddeti ile ilişkili değildir.
THE ROLE OF COOPERATIVES IN THE SMES EMPOWERMENT IN RURAL AREAS
Authors: Hayati Safaah Restuning , Khasanah Miftakhul 
Published: June 2019

Purpose: This study aims to analyze the role of Mitra Dhuafa Cooperative in Gemolong Sub-district of Sragen, Indonesia, in empowering rural microenterprise, and to analyze its success rate by measuring the correlation between micro financing and the level of capability, and poverty alleviation.
Methodology: The method used in this study is quantitative analysis. The data collection was obtained through a survey by distributing questionnaires to 100 members of Mitra Dhuafa Cooperative. The data was analyzed using Somers'd Correlation Test, with Statistical Package for Social Science (SPSS) software.
Main Findings: The result of data analysis shows a positive relation (0.320) between the level of capability of micro business actors and poverty alleviation. The Indicators of empowerment success were measured by Amartya Sen's capability indicators, namely, opportunities and access in the economic, social, health, education, openness, and security fields. The research concludes by stating that Mitra Dhuafa Cooperative has succeeded in reducing the level of poverty by increasing the capability, bargaining power and independence of micro business actors.
Applications: This study can be useful for implementing poverty alleviation program and Islamic microfinance programs in rural areas.
Novelty/Originality: The combined implementation of Grameen Bank model and Sen’s capability approach to analyze its success for poverty alleviation in rural areas.
POLITENESS IN ONLINE COMMUNICATION: RETAILER-CLIENT INTERACTION
Authors: Tengku Sharif Tengku Intan Suzila , Mohamad Noor Mohd Yusri , Omar Siti Rohana 
Published: June 2019

Purpose of the study: The paper aims to unfold politeness in online communication among retailers and clients and debates some ways where cultural values of communication can be taught and thus re-learned.
Methodology: The qualitative approach was adopted where discourse analysis method used to review and analyse the updates and comments from Facebook and WhatsApp of conveniently collected data for this study.
Main Findings: The study suggest a deterioration of cultural values in communication is at an alarming level among retailers and clients in online communication.
Applications of this study: The study will benefit online businesses. Area of studies include business management and communication. Other sub- areas include culture and ethic relations.
Novelty/Originality of this study: The study is new to Malaysian business context where multi-racial and multilingual environment play significant roles.
UTILIZATION OF MICROORGANISM ON THE DEVELOPMENT OF HALAL FOOD BASED ON BIOTECHNOLOGY
Authors: Faridah Hayyun Durrotul , Sari Silvi Kurnia 
Published: June 2019

Biotechnology has rapidly developed until now. The use of biotechnology was very extensive for human needs, including the food industry. Some biotechnological processes, both conventional modern, were inseparable from the use of microorganisms. A number of certain microorganisms were used in traditional food through fermentation such as yogurt, tape, cheese and salted vegetables. In addition, microorganisms also used in the DNA recombination process to produce high quality food products such as GMO (Genetically Modified Organism). Indonesia is a country with a majority of the Muslim population so that in the provision of food was very concerned about aspects of halal. Included in biotechnology foods that utilizing microorganism and have to pay attention of the halal critical point. In this case, needs to be considered so that every production process can be ensured to avoid contamination of non-halal materials
Pengaruh Durasi Waktu Pada Sexing Spermatozoa Sapi Bali Terhadap Kualitas Dan Efektivitas Sexing Spermatozoa Dengan Menggunakan Alat Electric Separating Sperm (ESS)
Authors: Ashari Lukman , Mustofa Imam , Yunita Maya Nurwartanti , Sardjito Trilas , Saputro Amung Logam , Prastiya Ragil Angga 
Published: June 2019

Tujuan penelitian ini adalah mengetahui perbedaan kualitas (viabilitas, motilitas dan abnormalitas) spermatozoa Sapi jantan Bali hasil sexing menggunakan alat ESS yang dialiri listrik selama lima menit dan sepuluh menit pada sisi anoda dan katoda dan mengetahui perbedaan efektivitas pemisahan spermatozoa Sapi Bali hasil sexing menggunakan alat ESS yang dialiri listrik selama lima menit dan sepuluh menit pada sisi anoda dan katoda. Penelitian ini menggunakan Sapi Bali yang berumur 4 tahun sampai 7 tahun dengan motilitas di atas 45% dan di bawah 60%. Penelitian ini menggunakan diluter tris kuning telur. Sexing spermatozoa menggunakan alat ESS yang dialiri listrik dengan durasi 5 menit dan 10 menit pada masing-masing anoda dan katoda. Hasil penelitian ini menunjukan kualitas (viabilitas, motilitas dan abnormalitas) mengalami penurunan. Kualitas terbaik pada sexing spermatozoa terdapat pada durasi lima menit. Efektivitas pemisahan spermatozoa yang paling baik yaitu pada durasi sepuluh menit pada sisi katoda spermatozoa X sebesar 62,17±0,240% dan pada sisi anoda spermatozoa Y sebesar 67,33± 1,03%.
Prognostic factors of key outcomes for motor neuron disease in a multiracial Asian population
Authors: Deng Xiao , Hao Ying , Xiao Bin , Tan Eng-King , Lo Yew-Long 
Published: June 2019

Abstract
Objective

To determine potential prognostic factors for survival, need for feeding and ventilation support in motor neuron diseases (MND) patients in a multi-racial Asian population.

Methods

One hundred and four MND patients from the Singapore General Hospital (SGH) between January 2004 and December 2017 were reviewed. All relevant clinical data, demographic information were collected. Kaplan-Meier and cox regression model were performed to identify potential prognostic factors for crucial outcomes (survival, need for feeding support and ventilation support).

Results

Mean age of onset was 59.54± 10.91 years, Mean age of onset in Malays was significantly younger than that of other ethnic groups (Malay: 54.18±12.95years; Non-Malay: 60.39±10.38years, p=0.035). Fifty six of the male and 33of the female were diagnosed with ALS (90.3% vs 78.6% p=0.048). Mean overall survival duration from symptom onset was significantly longer in female than male patients (female: 39.2± 29.04 months; male: 29.4± 24.06months, P=0.03).

Bulbar onset was a significant risk predictor for both the need of feeding support (HR=3.87, p<0.001) and respiratory aid (HR=3.43, p=0.01).A longer interval from symptom onset to diagnosis was correlated with a longer time to the need for feeding support (HR=0.94, p=0.002) and respiratory aid (HR=0.9, p=0.009).

In the univariable cox regression analysis, need for respiratory support (HR=2.1, p=0.03) indicated poor survival outcome significantly, while MND subtypes other than ALS (HR=0.25, p=0.008) and slower disease progression, including longer duration from symptom onset to second symptom (HR=0.97, p=0.005), to feeding support (HR=0.95, p<0.001), to ventilation support (HR=0.96, p=0.004), significantly suggested lower risk of death. In the multivariable cox regression model, bulbar onset (aHR=5.28, p=0.035) correlated with poor survival outcome while longer duration from onset to second symptom (aHR=0.96, P=0.037) indicated better survival.

Conclusions

Bulbar onset was a significant risk predictor for survival, need of feeding support and ventilation aid. Slower disease progression correlated with better outcomes. Age of onset may differ among ethnic groups. Male patients are more likely to develop ALS and have shorter survival duration.
The loop-mediated isothermal amplification technique to determine T. gondii infection in women with spontaneous abortion
Authors: Kheirandish Farnaz , Fallahi Shirzad , Mahmoudvand Hossein , Anbari Khatereh , Araban Ali , Rouzbahani Arian Karimi , Akbari Soheila 
Published: June 2019

Abstract
Background

Toxoplasmosis caused by Toxoplasma gondii is well-known as one of the most widespread parasitic diseases, which has now infected nearly a third of the world's population. Among the infectious agents, T. gondii is considered as one of the main causes of spontaneous abortion (SA). The present study aimed to use the loop-mediated isothermal amplification (LAMP) technique in comparison with serological tests to determine the rate of T. gondii infection in women suffering from SA in Lorestan Province, Western Iran.

Methods

A total of 140 women suffering from their first spontaneous abortion and who had been referred to the Obstetrics and Gynecology Department, Asalian hospital (Khorramabad, Iran) were included in this study. The collected aborted fetal remains and blood samples (5 ml) from each patient were examined in sterilized conditions using the LAMP technique and ELISA commercial kits to determine the specific IgM and IgG anti-Toxoplasma antibodies respectively. Moreover, a questionnaire about some demographic and risk factors, such as the mother’s age, gestational age, and contact with cats was completed for each patient.

Results

Of the 140 women, 80 (57.1%) tested seropositive for anti-T. gondii antibodies by ELISA, 72 (51.4%) women tested seropositive for the IgG antibody, 8 (5.7%) tested seropositive for the IgM antibody. Among the 8 women who’d had their first spontaneous abortion who tested seropositive for IgM antibody by ELISA, only 5 cases (62.5%) reported positively to the LAMP test. The difference in the frequency distribution of the LAMP results for measuring the Toxoplasma infection in pregnant women under study was statistically significant (P <0.001) from the results of the serological test (ELISA). Although there was a significant difference between age and positivity in the LAMP test (P = 0.017), no significant difference was observed between positivity in the LAMP test and residence, education, job, and contact with cats.

Conclusion

The findings of the present investigation suggest that LAMP is a preferred method for determining Toxoplasma infection in pregnant women suffering from spontaneous abortion compared with other routine serological tests.
Interaction of camel Lactoferrin derived peptides with DNA: a molecular dynamics study
Authors: Pirkhezranian Zana , Tahmurespur Mojtaba , Daura Xavier , Monhemi Hassan , Sekhavati Mohammadhadi 
Published: June 2019

Abstract
Background: Lactoferrampin (LFampin), Lactoferricin (LFcin), and LFchimera are three well-known antimicrobial peptides derived from Lactoferrin and proposed as alternatives for antibiotics. Although the intracellular activity of these peptides has been previously demonstrated, their mode of action is not yet fully understood. Here, we performed a molecular dynamics simulation study to understand the molecular interactions between camel Lactoferrin derived peptides, including CLFampin, CLFcin, and CLFchimera, and DNA as an important intracellular target. Results: Our results indicate that all three peptides bind to DNA, albeit with different propensities, with CLFchimera showing the highest binding affinity. The secondary structures of the peptides, modeled on Lactoferrin, did not undergo significant changes during simulation, supporting their functional relevance. Main residues involved in the peptide-DNA interaction were identified based on binding free energy estimates calculated over 200 ns, which, as expected, confirmed strong electrostatic interactions between DNA phosphate groups and positively charged peptide side chains. Interaction between the different concentrations of CLFchimera and DNA revealed that after binding of four copies of CLFchimera to DNA, hydrogen bonds between the two strands of DNA start to break from one of the termini. Conclusions: Importantly, our results revealed that there is no DNA-sequence preference for peptide binding, in line with a broad antimicrobial activity. Moreover, the results showed that the strength of the interaction between DNA and CLFchimera is concentration dependent. The insight provided by these results can be used for the rational redesign of natural antimicrobial peptides targeting the bacterial DNA.
De novo sequencing of the transcriptome of Fargesia macclureana (Poaceae) reveals regulators of the floral transition and ecological adaptations to high altitude
Authors: Li Ying , Zhang Chunxia , Yang Kebin , Shi Jingjing , Ding Yulong , Gao Zhimin 
Published: June 2019

Abstract
Background

Fargesia macclureana (Poaceae) is a woody bamboo species found on the Qinghai–Tibet Plateau (QTP) approximately 2,000 ~ 3800 m above sea level. It rarely blossoms in the QTP, but it flowered 20 days after growing in our lab, which is in a low-altitude area outside the QTP. To date, little is known regarding the molecular mechanism of bamboo flowering, and no studies of flowering have been conducted on wild bamboo plants growing in extreme environments. Here, we report the first de novo transcriptome sequence for F. macclureana to investigated the putative mechanisms underlying the flowering time control used by F. macclureana to adapt to its environment.

Results

Illumina deep sequencing of the F. macclureana transcriptome generated 140.94 Gb of data, assembled into 99,056 unigenes. A comprehensive analysis of the broadly, specifically and differentially expressed unigenes (BEUs, SEUs, and DEUs) and a weighted gene co-expression network analysis (WGCNA) revealed that changes in expressions of unigenes related to the circadian cycle may account for the differences in the floral transition of F. macclureana after being transplanted from the QTP to a laboratory outside. In addition, differences in active carbohydrate metabolism and signal transduction between the flowering and non-flowering plants. Moreover, we detected the expression of unigenes related to DNA repair and plant-pathogen interactions, which may be of adaptive importance. Finally, we detected 9,296 simple sequence repeats (SSRs) that may be useful for further molecular marker-assisted breeding.

Conclusions

F. macclureana may have evolved specific reproductive strategies for flowering-related pathways in response to photoperiodic cues to ensure long vegetation growing period. Our findings will provide new insights to future investigations into the mechanisms of flowering time control and adaptive evolution in plants growing at high altitudes.
Ocular Trauma Pattern in the North Bund Area of Shanghai Pinpoints Risk Factors and Predicts Visual Outcomes
Authors: Zhang Jian , Chen Shimei , Suo Yan , Yao Xieyi , Wei Fang , Chen Fenge 
Published: June 2019

Abstract
Background

To identify the ocular trauma pattern in the north Bund area of Shanghai.

Methods

This is a retrospective, cross-sectional study. Patients between January 2016 and December 2018 with trauma in one eye and being hospitalized for at least one day in Shanghai General Hospital were selected in this study. All patients received first evaluation at the emergency room, followed by careful eye examination by one of attending ophthalmologists and reevaluation by one of the authors. Demographics and related patient’s clinical data at the time when trauma occurred were recorded, including age, gender, occupation, cause and type of trauma, location and size of injury, type of surgery, and hospitalization time.

Results

206 patients were reviewed. We found that gender and age are two mutually correlated risk factors for ocular trauma. We also identified occupation as a major risk factor, which is closely associated with penetrating injury. Moreover, season change adds an additional layer of risk factor for ocular trauma, showing a significantly higher peak of ocular trauma incidence in hot summer months. Furthermore, RD and VH were identified as two prevailed types of complications. Importantly, we found that complications, along with location and/or size of injuries, determine the visual outcomes.

Conclusions

We identified a clear ocular trauma pattern in the north Bund area of Shanghai. Importantly, complications, location and/or size of injuries determine the visual outcomes. Our findings pinpoint risk factors for ocular trauma, which predict visual outcomes and might be further applied in eye injury prevention.
CYP1B1 variants have low contribution to Pakistani patients with Primary Open Angle Glaucoma Page
Authors: Shahani Muhammad Yaqoob , Memon Samreen , Waryah Ali Muhammad , Narsani Ashok Kumar , Bano Umbreen , Shaikh Shakeel Ahmed , Shahani Shazia Begum 
Published: June 2019

Abstract
Background

Glaucoma is a group of complex neurodegenerative ophthalmological disorders and is the second common of cause of irreversible blindness around the globe. Primary open-angle glaucoma (POAG) is the commonest type of all glaucoma and is characterized by elevated intraocular pressure (IOP) leading to optic nerve damage and visual field defects. Inherited mutations in CYP1B1 are a rare cause of POAG.

Objective

This study was conducted to screen Pakistani population with POAG for CYP1B1 variants.

Methods

Detailed family history was recorded from all participating families. The disease was confirmed through ophthalmological examinations. Blood samples were collected for genomic DNA extraction. CYP1B1 exons were directly sequenced in one affected individual from each family.

Results

CYP1B1 sequencing revealed a novel heterozygous missense variant, c.649G>A (p.Asp217Asn). All affected individuals having the variant had characteristic glaucomatous changes with mean disease onset at 35 years. The unaffected individuals in the family had no signs of POAG. The prevalence of CYP1B1 associated POAG in study cohort is 4% (1/25). In silico predictions showed that p.Asp217Asn, substitution affects the structure and function of the protein.

Conclusion

The study suggests that CYP1B1 mutations are considered to have have less contribution in pathogenesis of POAG in Pakistani population studied. Identification of novel dominantly inherited variant shows allelic heterogeneity and may help in early diagnosis for effective management of the disease through genetic counseling.
Vector analysis of Contoura Vision for the correction of myopia and myopic astigmatism
Authors: Lin Ying , Huanjun Su , Jianbin Wu , Xia Zhang , Xi Xu , Muzhi Yuan 
Published: June 2019

Abstract
Background: To evaluate the visual outcomes of Contoura Vision with automatic eye tracking system in eyes with myopia and myopic astigmatism.
Methods: In this prospective study, 40 consecutive patients (80 eyes) with moderate myopia and irregular astigmatism were included from January to August 2018 at Liuzhou Worker Hospital. Subjects were randomly divided into an experimental group (40 eyes) that underwent Contoura Vision FS-LASIK and a control group (40 eyes) that underwent wavefront-optimized FS-LASIK. Target refractions of all subjects were plano. Visual outcomes and astigmatic vector analysis were evaluated and compared between preoperatively and 3 months post-operatively.
Results: The preoperative spherical and cylindrical refractive errors were similar in both groups (P>0.05). At 3 months postoperatively, cumulative uncorrected distance visual acuity (UDVA) was 20/16, 20/20, or 20/25 in 30 %, 95%, 100 % of patients in the experimental group, respectively. The experimental group was better than the control group in predictability of astigmatism correction at 3 months after operation. In the experimental group, 80% of eyes had deviation of astigmatic axis within 15° and 35% of eyes had deviation of astigmatic axis within 5°, both were better than those in the control group. The number of eyes with residual astigmatism within 0.5D were higher in the experimental group (60%, 24 eyes) than the control group (50%, 20 eyes). Compared with the preoperative, C7 significantly reduced to 0.056±0.030 in the experimental group at 3 months after the procedure (P<0.05), and were significantly lower than those in the control group (P<0.05).
Conclusion: Contoura Vision with automatic eye tracking system was safe and effective for the correction of myopia and myopic astigmatism.