bluuebunny/crossref_metadata_2025_split huggingface dataset:
PL - 028 Aerobic exercise inhibits tau hyperphosphorylation through activation of the PI3K/Akt pathway in the hippocampus of APP/PS1 mice
Authors: Fang Guoliang , Zhao Jiexiu , Zhang Li , Li Pengfei 
Published: October 2018

Objective Many studies suggest that regular physical exercise can reduce the risk of Alzheimer’s disease and slow its onset and progression. However, the exact mechanism is still unclear. Clinically, Alzheimer’s disease is characterized by the presence of extracellular amyloid plaques and intraneuronal neurofibrillary tangles, which are associated with amyloid-β and tau hyperphosphorylation respectively. The PI3K/Akt signaling pathway regulates tau phosphorylation and plays a pivotal role in the development of pathology in Alzheimer’s disease. Here we investigated the effects of aerobic exercise on tau phosphorylation and examined whether these effects were mediated by the PI3K/Akt pathway in the hippocampus of APP/PS1 transgenic mice.
Methods 40 male APP/PS1 transgenic mice were randomly divided into four groups: sedentary group (T-SE; n=10), exercise group (T-EX; n=10), sedentary with GNE-317 treatment group (T-SEG; n=10) and exercise with GNE-317 treatment group (T-EXG; n=10). GNE-317 is a potent and selective PI3K/Akt pathway inhibitor that can cross the blood-brain barrier and show effective suppression of Akt phosphorylation in the mice brain. The mice in the T-EX and T-EXG groups were given exercise training on a treadmill for 5 days/week for 8 weeks with 0% grade, and progressively ran from 30 min/day at 12 m/min, up to 60 min/day at 15 m/min. The mice in the T-SE and T-SEG groups were placed individually on another treadmill at 0 m/min for the same duration. 48 hours after the last exercise bout, all mice were intraperitoneally injected an anesthetic for inducing anesthesia, and the hippocampus were rapidly extracted. The protein and phosphorylation levels of tau, PI3K, Akt and GSK3β were assayed by Western blot and immunohistochemistry. The cognitive function were tested by morris water maze.
Results We found out that 8 weeks of aerobic exercise reduced tau phosphorylation at multiple sites including Ser202, Thr231 and Ser396, and increased phosphorylation of Akt at Thr308 and Ser473 and of GSK3β at Ser9. Furthermore, in the morris water maze test, the exercise group showed a reduced escape time and distance compared with those of the sedentary group, suggesting that aerobic exercise improved learning and cognitive ability. While the above-mentioned results were attenuated in the PI3K/Akt inhibitor GNE-317 treatment groups.
Conclusions Our study demonstrated that aerobic exercise could inhibit tau hyperphosphorylation and improve cognitive funtion through activation of the PI3K/Akt pathway in the hippocampus of APP/PS1 mice.
OR-006 Effect of Moderate-intensity Exercise on the Expression of Hypothalamic KiSS-1 and GPR54 mRNA in Diet Induced Obesity Rats
Authors: Yan Yi , Liang Chunyu , Xu Rui 
Published: October 2018

Objective To observe the effect of high fat diet on the hypothalamic expression of KiSS-1and the G-protein coupled receptor (GPR) 54 mRNA and explore the modulatory role of moderate-intensity exercise in the diet induced obesity male rats.
Methods After 8 weeks high fat feeding, 20 obesity 11-weeks SD rats were randomly assigned to high-fat diet sedentary (FS, n=8) and high-fat diet exercise (FE, n=8) groups, 20 normal diet 11-weeks SD rats also were randomly assigned to sedentary (SS, n=8) and exercise (SE, n=8) groups. During the following 8 weeks, obesity rats were continued expose to high-fat-diet. SE and FE groups did the 60%-70%V(•)O2max treadmill training (5 days/week, 1 hour/day). The V(•)O2 max of exercise groups were remeasured every two weeks. The hypothalamic expression of KiSS-1 and GPR54 mRNA were tested in each group.
Results
After the first 8-weeks high fat feeding, the obesity rats were heavier than normal diet group (491.74±26.19g vs. 410.05±45.77g, p<0.01). After 8-weeks training, the FE group was lighter than FS group (590.23±35.74g vs. 681±52.56, p<0.01). The FS group had higher hypothalamic expression of KiSS-1 mRNA (1.51±0.66 vs 0.75±0.27, p<0.05) and GPR54 mRNA (2.45±0.38 vs 0.61±0.15, p<0.01) than SS group. The FE group had lower hypothalamic expression of KiSS-1 mRNA (0.69±0.13, p>0.05) and GPR54 mRNA (0.58±0.10, p<0.01) than FS group.
Conclusions
There is stimulating effect of high-fat diet induced obesity on hypothalamic expression of KiSS-1and GPR54 mRNA. 8-weeks 60%-70%V (•) O2max treadmill training could cure this effect.
OR-022 Effects of aerobic exercise on the hemodynamics and structure of the common carotid artery in obese adolescents
Authors: Shi Wenxia , Cao Ling , Xu Xianzhe 
Published: October 2018

Objective With the population of obese adolescents increases dramatically, a series of cardiovascular diseases, especially atherosclerotic, are triggered by obese which seriously threatens the life and health of teenagers. The aim of this study is to investigate the effects of aerobic exercise intervention on the hemodynamics and structure of the common carotid artery in obese adolescents.
Methods Forty obese adolescents (18 ± 2years) were randomly assigned into the experimental group (EG; n = 20) and control group (CG; n = 20). EG undertook 12 weeks of aerobic exercise training (AET), CG had not any exercise intervention. The carotid artery of both CG and EG were examined and compared. Carotid artery responses were assessed in both groups. Color doppler ultrasound was used to determine the tube diameter and axial flow of the common carotid before and after exercise intervention. The heart rate, systolic and diastolic blood pressure were simultaneously measured on the left brachial artery by a sphygmomanometer.
Results Compared with CG, there were improvements of EG in peripheral resistance (22.90±6.70 VS 29.58±8.71. p＜0.01) and Systolic blood pressure (123.57±7.36 VS 130.25±6.79. p＜0.05) were verified after AET, except diastolic blood pressure. Following AET, blood flow velocity (0.28±0.05 VS 0.21±0.05. p＜0.01) and wall shear stress (6.25±0.90 VS 4.97±1.54. p＜0.05) increased prominently, which were also significant differences only in EG. In contrast, the vascular diameter demonstrated consistently upper compared with CG, but no differences between EG and CG.
Conclusions Regular aerobic exercise lasting 12 weeks could effectively change the dynamic parameters of the common carotid artery in obese adolescents, but no changes in arterial diameter. These findings indicated that 12 weeks of aerobic exercise can induce some changes of the common carotid artery blood flow within the circulation function in a short time. But the changing in common carotid arteries structure is needed after a long-term blood flow to the stimulation.
OR-034 The parvalbumin positive neuron involved the regulation of motor cortex excitability in the exercise-induced fatigue
Authors: Cui Shuqiang , Qiao Decai , Liu Xiaoli 
Published: October 2018

Objective Objective:Cortical parvalbumin-expressing inhibitory neurons(PV) control the activity of excitatory neurons and regulate their spike output. The present experiment is to determine the role of PV neuron in the reglution of excitability of primary motor cortex (M1) during the exercise-induced fatigue and possible molecular mechanism.
Methods Methods: Male Wistar rats randomly divided into control group(C),exhaustive exercise group(E) and repeated exhaustive exercise group(RE). The gradually increasing load treadmill exercise-induced fatigue model was employed in the Group E and RE.The in vivo multi-channel recording methods was used for recording the neuronal electrophysiological activities of primary motor cortex.To observe the neuron firing rate changes during the rest state,immediately after exhausted exercise and after repeated exhaustive exercise.We also detected the expression of PV positive neurons in the primary motor cortex by the immunofluorescence method. The western blot method was used to determine the expression of calmodulin-dependent protein kinase II (CaMKII)、phosphorylated calmodulin-dependent protein kinase II( pCaMKII) and extracellular signal regulated kinase (ERK) in the primary motor cortex.
Results Results：The electrophysioligical results indicated that the neuron firing rate after repeated exhausted excise the neuron firing rate significantly decreased compared with the rest state (P<0.05),but have no significantly changes as compared with exhausted excise;The expression of PV positive neurons in the group of E and RE significantly increased compared with the group C(P<0.01);The western blot results indicated that the protein expression of ERK in group REsignificantly decreased compared with group C, the pCaMKII expression of group RE decreased,but have no statistical difference.
Conclusions Conclusion: After exercise-indued fatigue ,the increase of PV positive neuron maybe one reason for the excitability changes in primary motor cortex.the alteraions in the electrical signal may be participate in the regluation of exercise-induced fatigue. pCaMKII and ERK signal pathway may invloved in the molecular mechanism of exercise-induced fatigue.
OR-045 Comparative Study on Physical Fitness in Female College Students between Different BFP Grades
Authors: Wang Hui , Zhang Chognlin , Ding Xiaomin , Liu Shaosheng 
Published: October 2018

Objective Through testing the body fat percentage (BFP) and physical fitness by laboratory methods in female college students, the relationship between physical fitness and body morphology of female college students was revealed.
Methods BFP and fat free mass were measured among 633 healthy female college students randomly selected. All subjects were divided into five groups by their BFP: low, normal, high body fat, grade I obesity and grade II/III obesity. Grip Strength, Leg Strength, Back Strength, Vital Capacity, Maximal Oxygen Uptake, Reaction Time, Sit And Reach, Back Scratch and Standing On One Foot With Closed Eyes were tested.
Results There were significant differences between all groups in Grip Strength, Back Strength, Vital Capacity, the absolute and relative value of VO2max, Back Scratch on both sides and Standing on One Foot with Closed Eyes. The difference in Grip Strength and Back Strength showed significantly (P < 0.05), and the others showed very significant difference (P < 0.01). There was no significant difference in Resting Heart Rate, Reaction Time, Sit And Reach and Leg Strength. The results of multiple comparison showed that compared with 4 group,there were significant differences in Vital Capacity, relative value of Maximum Oxygen Uptake, Back Scratch in both sides in 2 group and 3 group. Moreover, compared with 5 group, there were significant differences in all the indicators in 2 group and 3 group. And there were significant differences in all the indicators except Back Strength and the relative value of Maximum Oxygen Uptake between 5 group and 4 group.
Conclusions The results showed that the physical fitness of female college students with normal BFP was significantly better than that of obese ones. The major influence of exceeding 30 percent in BFP was the cardiopulmonary endurance fitness and upper limb flexibility fitness. In the group of BFP higher than 35%, these influences were more significant, meanwhile, balance fitness declined.
OR-048 Effects and Mechanism of myokines in exercise mediated improving obesity rats skeletal muscle remodeling
Authors: Zhang Liang , Yang Hongtao , Ma Jin , Li Linjie 
Published: October 2018

Objective Aims From the year 2000, many experimental research data have indicated that skeletal muscle could express, synthesis and secrete multiple cytokines and polypeptides. The cytokines and polypeptides, not only regulate skeletal muscle growth, metabolism and motor function by paracrine/autocrine pathway, but also regulate functions of peripheral tissue and organs by endocrine pathway. Further researches proposed muscle as a secretory organ played a key role in mediating the health-promoting effects of physical activity and proteins expressed and released by skeletal muscle have been termed as myokines. Disorders of skeletal muscle endocrine function have related to the occurrence and development of multiple metabolic diseases, and myokines participate in obesity skeletal muscle remodeling. This study aims to investigate the expression changes of myokines and its effects in exercise mediated improving skeletal muscle remodeling on obesity mice, and explore the underlying mechanism of its functions.
Methods Methods Five-week-old male Sprague-Dawley(SD) rats were randomly divided into a control group of 8 and a high-fat diet（HFD）group of 16. The control group was given normal food，while the HFD group were provided with high-fat diet for eight weeks and further divided into a sedentary HFD group and a treadmill running HFD group，each of 8. The exercise mice underwent 60 min treadmill running at 26 m/min each day，5 days/week for 8 weeks. Biochemical analyses, immune-histochemical, ELISA, RT-PCR and Western Blot methods were used to investigate multiple myokines expression changes and its mechanism.
Results Results 1) Exercise significantly upregulated the expression of IL-15 in soleus and gastrocnemius muscle of obesity rats, indicating IL-15 could inhibit skeletal muscle endoplasmic reticulum stress and improve insulin sensitivity. 2) Exercise significantly inhibited the expression of myostatin (MSTN) in gastrocnemius muscle and mediated the changes of muscle fiber types. 3) Exercise markedly promoted the expression of apelin/APJ and angiogenesis function in obesity skeletal muscle. 4) Exercise upregulated skeletal muscle vascular endothelial growth factor B receptor expression and improved skeletal muscle ectopic lipid accumulation.
Conclusions Conclusion Exercise regulates skeletal muscle myokines expression and secretion and have the effects on skeletal muscle fiber type changes, myofiber capillary density, glucose and lipid metabolism, thus improves the skeletal muscle remodeling and maintain body homeostasis.
PO-037 Validation Of Capillary Blood Gas Analysis For The Assessment Of Training Load In Track Cycling
Authors: Ma Guoqiang , Tang Qi , Ni Dahai , Parsons Brentan 
Published: October 2018

Objective The energy supply of daily training of track cycling should be mainly by anaerobic metabolism, which can make the blood buffer capacity facing huge Challenge and being improved. The arterialized capillary blood gas analysis can be a reliable method to evaluate the blood status of base-acid balance, which may reflect the effect of exercise intensity on blood buffer system. The paper validate the blood gas analysis as a reliable method for evaluating the total periodical training load of track cyclists.
Methods Five male and five female elite track cyclists performed two phases (four weeks per phase) of training respectively. The content included the track specific, strength and aerobic training. The Borg's Rating of Perceived Exertion (RPE) Scale (0-11) was recorded in the ten minutes after each session and calculated the session RPE (sRPE). The total inertial load (TIL) was calculated by sRPE sum from Monday to Thursday. The arterialized capillary blood gas analysis was performed at 7:00 AM on Monday and Friday. The delta value (ΔPH, ΔPCO2, ΔTCO2, ΔHCO3-, ΔBE, ΔPO2, ΔSO2) were calculated by Friday minus Monday. Pearson's linear correlation was applied to calculate the correlation between TIL and delta value. Independent t test was used to test the differences between two genders.
Results There was the moderate correlation between TIL with ΔPH and ΔHCO3- (Correlation Coefficient= 0.712 and 0.642 respectively, P<0.01). But other blood gas indexes didn’t show the obvious relationship with TIL (Correlation Coefficient < 0.5). There was no differences for TIL between male and female (3870.1±788.4 vs. 4130.2±716.7, P>.05). Moreover, ΔHCO3- of male was significant more than female by 95.1% (P<0.01). There were significant correlation between TIL and ΔPH for both male and female (Correlation Coefficient= 0.785 and 0.812 respectively, P<0.01), and between TIL and ΔPH for both male and female (Correlation Coefficient= 0.662  and 0.658 respectively, P<0.01).
Conclusions The PH value and bicarbonate radical of blood should be sensitive to the high intensity track cycling training, which can be the valid to evaluate the inertial load. However, gender has no influence on evaluating. The oxygen partial pressure and oxygen saturation of blood can not reflect the training load accurately.
PO-039 Influence of Physical Activity on Aerobic Endurance Capacity on Primary School Students
Authors: Chen Mengjiao , Jin Nannan , Fan Fangzheng , Bi Yan , Ding Tiancui , Hou Lijuan 
Published: October 2018

Objective The physical fitness of primary school students is not optimistic in recent years. The obesity rate and myopia rate have continuously increased, and the aerobic endurance capacity has insistently declined. The aerobic endurance capacity is an important indicator to evaluate the physical fitness of students. This article explores the effects of physical activity(PA) designed by NSPEL on the improvement of aerobic endurance by intervening in the fourth grade students of Shifoying Primary School in Chaoyang District in Beijing, and provides a reference for students' physical fitness promotion.
Methods 155 fourth-grade students from Shifoying primary School in Beijing were selected as subjects. They were subjected to PA intervention for 16 weeks and their physical fitness related indicators were evaluated. PA intervention includes in-class and extracurricular sports activities. Physical education was guided by the syllabus. Extracurricular sports activities were mainly activities in break and after-school sports interest classes. The final analysis of the physical fitness assessment scores, BMI, 50m run, 50-meter shuttle run, skipping and sit-up were performed before and after the intervention.
Results The average score of physical fitness assessment of students was 79.79 before PA intervention, and the score was significantly increased and increased to 88.12 (10.43%) after the intervention. Physical shape of the students changed significantly after PA intervention. The mean of BMI was within the normal range before and after intervention, and the obesity rate was reduced by 1.29%. The student's speed capacity improved significantly after the intervention, the average score of the 50-meter race increased from 10.22 seconds to 7.90 seconds, the difference was very significant, with an increase of 2.32 seconds after the intervention. The student's aerobic endurance capacity was significantly improved before and after PA intervention. The average score of the 50 meter shuttle run was increased from 2.01 min to 1.74 min, and the result was significantly improved by 13.43%. The average score of sit-up was 21.64, and it increased to 42.30 after the intervention, with an average increase of 20.66.
Conclusions Students' physical fitness assessment scores improved significantly after PA in and out of class, suggesting that PA effectively improved the physical fitness status of 11-year-old pupils. Students' 50-meter run, 50 meter shuttle run, skipping, and sit-ups were all significantly improved after PA interventions, suggesting that PA is of great significance for improving aerobic endurance. The combination of activities inside and outside class can be used as a reference mode to improve students' physical fitness (NSFC31401018, SKXJX2014014, Corresponding_houlj@bnu.edu.cn).
PO-040 IDEEA Estimation of Daily Behavior Energy Consumption: Verified by Metabolic Chamber
Authors: Lu Qi , Zhu Xiaomei , AI Lei , Chen Junfei , Liu Ling 
Published: October 2018

Objective To investigate the accuracy of Intelligent Device for Energy Expenditure and Activity (IDEEA) in the measurement of energy consumption in the evening of adults in Nanjing.
Methods 120 subjects were selected, and worn IDEEA into the metabolic chamber for 11 hours of energy consumption test.Body composition by Dual-Energy X-ray Absorptiometry. Sleep metabolic rate（SMR）,Rest metabolic rate （RMR）and Basal metabolic rate（BMR）by metabolic chamber.
Results The energy consumption results were paired with sample T test. The results showed significant difference between MC and IDEEA, but the effect size was between 0.004 and 0.042. The correlation between MC and IDEEA was 0.85 to 0 .96. The absolute error rate of energy consumption measurement was from 6.16 % to 7.92 %, of which the measurement error of sleep energy consumption was 6.16 %±4.16 %, and that of Internet energy consumption was 7.92 %±5.99 %.
Conclusions Energy measurement of IDEEA absolute error rate is within acceptable range, and it provides a high-precision alternative method for estimating energy consumption. The immediate and cumulative energy consumption data can be used to estimate the energy consumption for human physical activities over a period of time.
PO-51 Status of Relationship of Risk of Gestational Diabetes Mellitus and Physical Activity intervention
Authors: Guo Xian , Wang Yi 
Published: October 2018

Objective Gestational diabetes mellitus is the first time or any degree of glucose tolerance abnormalities discovered during the pregnancy. In the global the incidence of GDM can reach 15%. In China also reached 6.6%. Numerous studies have demonstrated that lifestyle intervention for the pregnant woman, especially to increase physical activity can significantly reduce the risk of GDM. Our research aims to search and analyze researches about the relationship of risk of gestational diabetes mellitus and physical activity intervention. Then we clear the effect of physical activity intervention and provide some advices for the future in this field.
Methods The literature data were used though Pubmed, Web of Science, EBSCO, CNKI et al. database and we searched with the keywords of “physical activity”, “exercise”, “exercise intervention”, “gestational diabetes mellitus”, “glucose tolerance”, “postpartum diabetes prevention” in all these database. Finally we reviewed researches on physical activity interventions in GDM patients, discussed the relationship of before pregnancy, during pregnancy and postpartum physical activity intervention between the GDM risk. And we used the comparative analysis to get the corresponding conclusions.
Results 1. Women of European and American countries during pregnancy who participated in physical activity and achieve the recommended amount was common, but only about 1/5 of Chinese women can achieve the physical activity amount which was recommended. 2. Join in regular physical activity before, during and after pregnancy, women could reduce the risk of GDM. 3. Aerobic training and/or resistance training could effectively improve glucose metabolism and insulin in patients with GDM and the recommended exercise prescription was moderate intensity, 3 to 5 days a week, last longer than 6 weeks.
Conclusions The incidence of GDM is closely connected with pregnancy lifestyle changes. Women take part in regular physical activity before, during and after pregnancy could reduce the risk of GDM. But this kind of research is rare and behindhand in China. In the future, we should put forward the corresponding suggestions for our country to make pregnancy physical activity guidelines. In the other hand, researchers should verify the effect of health of pregnant women after the exercise intervention.