bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigating the clinical significance of OAS family genes in breast cancer: an in vitro and in silico study
Authors: Lu Jinjun , Yang Lu , Yang Xinghai , Chen Bin , Liu Zheqi 
Published: December 2024

Abstract
Background
Breast cancer is the most common malignancy among women worldwide, characterized by complex molecular and cellular heterogeneity. Despite advances in diagnosis and treatment, there is an urgent need to identify reliable biomarkers and therapeutic targets to improve early detection and personalized therapy. The OAS (2′-5′-oligoadenylate synthetase) family genes, known for their roles in antiviral immunity, have emerged as potential regulators in cancer biology. This study aimed to explore the diagnostic and functional relevance of OAS family genes in breast cancer.

Methodology
Breast cancer cell lines and controls were cultured under specific conditions, and DNA and RNA were extracted for downstream analyses. RT-qPCR, bisulfite sequencing, and Western blotting were employed to assess gene expression, promoter methylation, and knockdown efficiency of OAS family genes. Functional assays, including CCK-8, colony formation, and wound healing, evaluated cellular behaviors, while bioinformatics tools (UALCAN, GEPIA, HPA, OncoDB, cBioPortal, and others) validated findings and explored correlations with clinical data.

Results
The OAS family genes (OAS1, OAS2, OAS3, and OASL) were found to be significantly upregulated in breast cancer cell lines and tissues compared to normal controls. This overexpression was strongly associated with reduced promoter methylation. Receiver operating characteristic (ROC) analysis demonstrated high diagnostic accuracy, with area under the curve (AUC) values exceeding 0.93 for all four genes. Increased OAS expression correlated with advanced cancer stages and poor overall survival in breast cancer patients. Functional analysis revealed their involvement in critical biological processes, including immune modulation and oncogenic pathways. Silencing OAS genes in breast cancer cells significantly inhibited cell proliferation and colony formation, while unexpectedly enhancing migratory capacity. Additionally, correlations with immune cell infiltration, molecular subtypes, and drug sensitivity highlighted their potential roles in the tumor microenvironment and therapeutic response.

Conclusion
The findings of this study established OAS family genes as potential biomarkers and key players in breast cancer progression, offering promise as diagnostic biomarkers and therapeutic targets to address unmet clinical needs.
Lipid disturbances induced by psychotropic drugs: clinical and genetic predictors for early worsening of lipid levels and new-onset dyslipidaemia in Swiss psychiatric samples
Authors: Delacrétaz Aurélie , Sadler Marie , Gamma Franziska , Preisig Martin , Richard-Lepouriel Hélène , von Gunten Armin , Conus Philippe , Plessen Kerstin Jessica , Kutalik Zoltan , Eap Chin B. 
Published: December 2024

Background
Early worsening of plasma lipid levels (EWL; ≥5% change after 1 month) induced by at-risk psychotropic treatments predicts considerable exacerbation of plasma lipid levels and/or dyslipidaemia development in the longer term.

Aims
We aimed to determine which clinical and genetic risk factors could predict EWL.

Method
Predictive values of baseline clinical characteristics and dyslipidaemia-associated single nucleotide polymorphisms (SNPs) on EWL were evaluated in a discovery sample (n = 177) and replicated in two samples from the same cohort (PsyMetab; n1 = 176; n2 = 86).

Results
Low baseline levels of total cholesterol, low-density lipoprotein cholesterol (LDL-C) and triglycerides, and high baseline levels of high-density lipoprotein cholesterol (HDL-C), were risk factors for early increase in total cholesterol (P = 0.002), LDL-C (P = 0.02) and triglycerides (P = 0.0006), and early decrease in HDL-C (P = 0.04). Adding genetic parameters (n = 17, 18, 19 and 16 SNPs for total cholesterol, LDL-C, HDL-C and triglycerides, respectively) improved areas under the curve for early worsening of total cholesterol (from 0.66 to 0.91), LDL-C (from 0.62 to 0.87), triglycerides (from 0.73 to 0.92) and HDL-C (from 0.69 to 0.89) (P ≤ 0.00003 in discovery sample). The additive value of genetics to predict early worsening of LDL-C levels was confirmed in two replication samples (P ≤ 0.004). In the combined sample (n ≥ 203), adding genetics improved the prediction of new-onset dyslipidaemia for total cholesterol, LDL-C and HDL-C (P ≤ 0.04).

Conclusions
Clinical and genetic factors contributed to the prediction of EWL and new-onset dyslipidaemia in three samples of patients who started at-risk psychotropic treatments. Future larger studies should be conducted to refine SNP estimates to be integrated into clinically applicable predictive models.
A longitudinal study of healthcare workers’ mental health during Western Australia's unique policy response to COVID-19
Authors: Newnham Elizabeth A. , Mergelsberg Enrique L. P. , Stanley Susanne , Hood Sean , Tearne Jessica , Celenza Antonio , Stevenson Teresa , Mavaddat Nahal , Demore Gavin , Kavanagh Hyranthi , McEvoy Peter M. 
Published: December 2024

Background
Western Australia's response to the COVID-19 pandemic was swift and effective in implementing public health protections and preventing the spread of the virus for the first 2 years. However, healthcare staff continued to be at increased risk of mental health concerns.

Aims
To investigate the longitudinal patterns of post-traumatic stress symptoms (PTSS), depression and anxiety among healthcare workers in Western Australia, and the risk and protective factors associated with changes in status during the first wave.

Method
Participants comprised 183 healthcare staff working at tertiary hospitals and major clinics across Perth, for whom longitudinal data were available. Questionnaire data were collected before Western Australia's first major COVID-19 community wave in early 2022 and following the first wave in late 2022. Online surveys comprised validated measures assessing psychological symptoms, risk and protective factors, and original measures of workplace factors.

Results
Overall rates of PTSS, depression and anxiety remained stable across the two assessment points. However, latent growth models revealed that those with lower PTSS, depression or anxiety symptoms at baseline reported a larger increase in symptoms over time, and those with higher symptoms at baseline had a smaller decline over time, indicating a ‘catch-up’ effect. Workplace stressors, sleep difficulties and trauma exposure were key risk factors for changes in psychological symptoms from baseline, and workplace and social supports played protective roles.

Conclusions
Improvements in systemic workplace factors are needed to support healthcare workers’ mental health during periods of acute stress, even in settings with high levels of emergency preparedness.
Clinical and neurocognitive profiles of a combined clinical high risk for psychosis and clinical control sample: latent class analysis
Authors: Stüble Miriam , Schultze-Lutter Frauke , Kaess Michael , Franscini Maurizia , Traber-Walker Nina , Walger Petra , Schimmelmann Benno G. , Vogeley Kai , Kambeitz Joseph , Kindler Jochen , Michel Chantal 
Published: December 2024

Background
The clinical high-risk (CHR) state for psychosis demonstrates considerable clinical heterogeneity, presenting challenges for clinicians and researchers alike. Basic symptoms, to date, have largely been ignored in explorations of clinical profiles.

Aims
We examined clinical profiles by using a broader spectrum of CHR symptoms, including not only (attenuated) psychotic, but also basic symptoms.

Method
Patients (N = 875) of specialised early intervention centres for psychosis in Germany and Switzerland were assessed with the Schizophrenia Proneness Instruments and the Structured Interview for Psychosis-Risk Syndromes. Latent class analysis was applied to CHR symptoms to identify clinical profiles. Additionally, demographics, other symptoms, current non-psychotic DSM-IV axis I disorders and neurocognitive variables were assessed to further describe and compare the profiles.

Results
A three-class model was best fitting the data, whereby basic symptoms best differentiated between the profiles (η2 = 0.08–0.52). Class 1 had a low probability of CHR symptoms, the highest functioning and lowest other psychopathology, neurocognitive deficits and transition-to-psychosis rate. Class 2 had the highest probability of basic and (attenuated) positive symptoms (excluding hallucinations), lowest functioning, highest symptom load, most neurocognitive deficits and highest transition rate (55.1%). Class 3 was mostly characterised by attenuated hallucination, and was otherwise intermediate between the other two classes. Comorbidity rates were comparable across classes, with some class differences in diagnostic categories.

Conclusions
Our profiles based on basic and (attenuated) psychotic symptoms provide clinically useful entities by parsing out heterogeneity in clinical presentation. In future, they could guide class-specific intervention.
Circulating inflammatory and neurotrophic markers as moderators and/or mediators of cognitive remediation outcome in people with bipolar disorders
Authors: Strawbridge Rebecca , Tsapekos Dimosthenis , Young Allan H. 
Published: December 2024

Background
Immune dysregulation appears involved in affective disorder pathophysiology. Inflammatory biomarkers have been linked with the cognitive impairment observed in people with bipolar disorders and as such are candidate markers that may improve with, and/or predict outcomes to, cognitive remediation therapies (CRT).

Aims
Nine candidate biomarkers were examined as putative mediators and/or moderators to improvements following CRT compared with treatment as usual (TAU) from a randomised controlled trial.

Method
Euthymic adults with bipolar disorders who had been randomised to CRT (n = 23) or TAU (n = 21) underwent blood testing before and after a 12 week intervention period. Five cytokines and four growth factor proteins, selected a priori, were examined in association with global cognition and psychosocial functioning outcomes.

Results
CRT attenuated a reduction in the brain-derived neurotrophic factor (BDNF), basic fibroblast growth factor and vascular endothelial growth factor-C compared to TAU. For the BDNF, lower baseline levels predicted better functional outcomes across the sample but was more pronounced in TAU versus CRT participants and indicated larger CRT effects in those with a higher BDNF. A moderation effect was also apparent for tumour necrosis factor-β and interleukin-16, with greater CRT versus TAU effects on functioning for participants with lower baseline levels.

Conclusions
Although preliminary, results suggest that CRT may exert some protective biological effects, and that people with lower levels of neurotrophins or cytokines may benefit more from CRT. We note an absence of associations with cognitive (versus functional) outcomes. These findings require further examination in large well-controlled studies.
Petting the Rabbit: The Favourite (2018), Anti-heritage, and the Search for Lesbians in the Past
Authors: Myers Jacob 
Published: December 2024

Abstract
Yorgos Lanthimos's The Favourite (Ireland/UK/US, 2018) centers Queen Anne's torturous relationship with two women administrators, seeming to promise clarity about the monarch's sexual history. Instead, the film obliquely addresses British state history while the camera's gaze focuses on the queen's fictional rabbits. Gradually, these animals overwhelm the film's narrative, turning the cinematic image into an undulating, grey morass. This essay meditates on why The Favourite endlessly reproduces the rabbits’ image and expands on how searching for lesbians in the past on film can mystify historical meaning. This article argues The Favourite constitutes an anti-heritage film, a film that uses heritage film's own generic dictates to unwrite history. Bringing together the film's reception, Queen Anne's historiography, and theories of sexual/cinematic reproduction, this essay outlines how rabbit proliferation at the site of lesbian desire subverts the heritage film's impulse to render the past into historical narrative. The film deploys visual copies of woman and rabbit that enliven residual anxieties about ungovernable feminine sexuality. In doing so, it marks the limits of the archive and encourages finding present life from outside the images proposed by national history.
Archaeological Narrative; or, Nostalgic Fascination with the Obsolete in the South Korean TV Drama Reply 1988
Authors: Kang Kyoung-Lae 
Published: December 2024

Abstract
This article considers how a Korean television drama, Reply 1988 (tvN, 2015–16), functions as a form of public archaeology through its on-screen display of obsolete TV sets and televisual footage, treating television as a historical medium to illuminate the lives of those whom television has influenced since the 1980s. Reply 1988 also extends this recollected medium into emerging hypermedia and intertextual contexts, enabled primarily through contemporary digital technology. Viewers therefore find themselves in a puzzle-like structure—a temporal doubling. This raises questions about television's development in today's rapidly changing media environment. This article examines Reply 1988 as an archaeological narrative to seek to understand the show's response to cultural crises playing out in today's post-network, post-media era, casting new light on television's ethical role in generating communal values and informing an inquiry into the human condition in the contemporary moment.
Surviving the Reset: Speedrunning Spyro, Trans Mundanity, and Playing in the Impasse
Authors: Schmalzer Madison 
Published: December 2024

Abstract
This article explores the relationship between everyday life and play, focusing on the mundane play practices of speedrunning. Employing autoethnographic techniques, the author analyzes her own experiences speedrunning and transitioning as case studies, drawing out the temporalities and affective dimensions of both, as well as the ways media practices and subjectivity become intertwined. To be specific, the article examines the experience of living in the impasse, which is brought on by temporal engagements, such as the reset and the grind, with videogames. Living in the impasse is characterized by prolonged waiting periods inherent to both trans life and videogame play and gives rise to negative affects like boredom, frustration, and pain. Ultimately, the author argues that these negative affects become enfolded into identity and serve as trans tools of survival.
Trends and Outcomes in Pituitary Apoplexy Management: A Spanish Observational Multicenter Study
Authors: Biagetti Betina , Cordero Asanza Esteban , García-Feijoo Pablo , Araujo-Castro Marta , Rodríguez Berrocal Víctor , Serra Guillermo , Guerrero-Pérez Fernando , Cámara Rosa , Lamas Cristina , Ollero García M Dolores , Vicente Almudena , Irigaray Echarri Ana , Villar-Taibo Rocío , Moure Rodríguez María Dolores , Pérez-López Carlos , González-Molero Inmaculada , Sánchez Ramírez María Noelia , Gutiérrez Hurtado Alba , Capristan-Díaz Vanessa , Simó-Servat Andreu , Gallach Marta , Safont Pérez Eva , González Rosa Victoria , Civantos Soralla , Martinez-Saez Elena , García-Arabehety Julia , Menéndez Torre Edelmiro , Aulinas Anna , Iglesias Pedro , Díez Juan J. , Bernabéu Ignacio , Álvarez-Escolá Cristina , Puig-Domingo Manel ,   On behalf of Neuroendocrinology Area of the Spanish Society of Endocrinology
Published: December 2024

BACKGROUND AND OBJECTIVE:
Currently, the management for pituitary apoplexy (PA) has been promoted toward a more conservative approach, particularly for patients with low-grade PA scores. Our aim was to investigate trends in PA management and compare clinical presentation, therapeutic approaches, and outcomes before and after 2017, additionally to evaluate long-term outcomes in conservatively treated patients.

METHODS:
Spanish multicenter, retrospective study. Statistical analyses compared clinical presentation and outcomes between periods, adjusting for confounders.

RESULTS:
A total of 215 patients with PA and nonfunctioning pituitary adenoma were included, with the median age of 62.2 years and 68.4% were male patients. Of which 94 (43.7%) were diagnosed before 2017 and 121 (56.3%) in 2017 or later. Conservative treatment increased from 17% before to 30.6% in the recent period (odd ratio 0.47, 95% CI 0.24-0.90, P 0.02) This trend remained significant after adjusting for hospital, age, and Knosp grade (odd ratio 0.46, 95% CI 0.22-0.89, P = .03). However, surgery remained the most frequently used therapeutic option in both periods. There were no statistically significant differences in outcomes at 3 months between periods. Surgery compared with conservative management was associated with higher permanent arginine vasopressin deficiency in both periods (0 vs 17.9%, P = .07 before 2017; 0 vs 16.7%, P = .01 after). Up to 89.7% of patients treated conservatively, presented more than 25% spontaneous tumor shrinkage, and 74.4% had more than 50% tumor reduction.

CONCLUSION:
Although conservative management increased in the last years, surgery remains the predominant option. Patients managed conservatively experience a lower risk of permanent arginine vasopressin deficiency, and a high proportion exhibit clinically significant tumor shrinkage over time.
Phantom pain - etiopathogenesis and treatment methods
Authors: Olszak Joanna , Zalewa Karolina , Bartoszek Lidia , Orłowska Dominika , Kapłan Wojciech , Poleszczuk Mikołaj , Czuba Anna , Poleszczuk Karol , Milewska Alicja 
Published: December 2024

Introduction and Purpose
Phantom limb pain (PLP) is a complex and multifactorial phenomenon that arises following limb amputation, affecting a significant number of patients. This study aims to explore the underlying mechanisms of PLP, assess current treatment strategies, and evaluate the effectiveness of various therapeutic interventions, including pharmacological and non-pharmacological approaches.
Material and Methods
A comprehensive literature review was conducted using the PubMed database, focusing on articles published up to the end of 2023. The search included the keywords: "amputation", "mirror therapy", "pain", "phantom limb", "amputation-induced pain", "chronic pain" in various combinations. Relevant studies were selected based on criteria such as the etiopathogenesis of phantom pain and its treatment methods.
Results
The findings indicate that PLP is associated with both peripheral and central nervous system changes, influenced by individual risk factors such as genetics and psychological state. Therapeutic interventions such as mirror therapy, graded motor imagery, and neuromodulation have shown promise in alleviating symptoms, although the evidence for their effectiveness remains variable. The implementation of a multidisciplinary approach significantly improves outcomes in managing PLP.
Conclusions
Effective management of phantom limb pain requires a comprehensive understanding of its etiology and a tailored treatment strategy that incorporates both pharmacological and non-pharmacological methods. Future research should focus on establishing robust clinical protocols and long-term efficacy studies to enhance the quality of life for individuals suffering from PLP.
FKBP5 gene - current knowledge, new approach and possible biomarker function: a narrative review
Authors: Solisch Sven , Boczar Agata , Dryja Patryk , Solisch Bianka , Jarmołowicz Jakub 
Published: December 2024

Introduction
Over the past few decades, the level of interest in the use of complex molecular pathways in the course of various diseases has increased significantly. Despite the rapid development of medicine, the potential and role of FKBP5 as a biomarker has not yet been fully characterized, although current studies are promising.

Material and methods of research: The literature included in the PubMed databases is searched through the words such as FKBP5 gene, stress-related disorders, biomarkers.

Aim of the study
The aim of this paper is to provide a comprehensive review of the current state of knowledge regarding the characteristics, current use in diagnosis or treatment of various diseases and to determine the possible function of the biomarker for the FKBP5 gene.

Description of the state of knowledge
FKBP5 participates in the regulation of the HPA axis and thus in the stress response. This translates into the course of many diseases, but the exact mechanism of action has not been unequivocally confirmed in previous studies on all conditions involving FKBP5.

Conclusions
In general, FKBP5 has a huge diagnostic and therapeutic potential in many disease entities. FKBP5 may act as a biomarker. However, further studies are needed to clarify the current observations and to search for further implications in the pathogenesis or diagnostic and therapeutic procedures in the course of various diseases.
The Language Contestations and Complexities at a South African Higher Education Institution: Students’ Perspectives
Authors: Sibanda Brian , Joubert Michelle 
Published: December 2024

Abstract
This article examines the complex discourse around language at one South African higher education institution, viewing it through the experiences of students, and drawing connections to the seminal 1962 Makerere conference. The noted exchange at Makerere between literary giants, Chinua Achebe and Ngugi wa Thiong’o who debated the merits of colonial versus native languages, remains pertinent to the ongoing discussions about decolonisation and the creation of knowledge in academic circles, especially within the Global South. Adopting a qualitative research approach with the use of focus groups, the article investigates student perspectives on the sensitive subject of language use in academia at the University of the Free State in South Africa, emphasising the roles of both English and Indigenous languages in education. Bourdieu’s concept of habitus, and more specifically linguistic habitus, provided the framework to interpret student responses to questions about language decolonisation. The framework was also used to explore students’ thoughts on how social structures and historical contexts shape their views concerning the adoption of local languages in higher education. The study underscores the significance of incorporating local languages into academic realms while also recognising the ambivalent nature of English as both a facilitator and barrier to learning. Centring student views, the study advances the discourse on multilingualism in higher education, pushing for a balanced perspective that acknowledges linguistic diversity and confronts dominant hegemonic language norms. Furthermore, it advocates for higher education institutions to develop inclusive language practices that enhance linguistic justice. In the results and discussion section, student views on the advantageous aspects of English are articulated, noting its utility; students’ views of the significance of their native languages in the pedagogic space are also explored. This dynamic between English and Indigenous languages underlines the persistent challenges in the debate over language and decolonisation in higher education. Lastly, the article emphasises the need to delicately handle the intricacies of linguistic choices in academia, balancing the functional benefits of English with the cultural and educational value of Indigenous languages. By elevating the experiences and viewpoints of students, the research contributes to evolving language policies that are both inclusive and just, and it supports the advancement of a genuinely multilingual academic world.