bluuebunny/crossref_metadata_2025_split huggingface dataset:
Taste Perception and Cerebral Activity in the Human Gustatory Cortex Induced by Glucose, Fructose, and Sucrose Solutions
Authors: Mouillot Thomas , Barthet Sophie , Janin Lucie , Creteau Camille , Devilliers Hervé , Brindisi Marie-Claude , Penicaud Luc , Leloup Corinne , Brondel Laurent , Jacquin-Piques Agnès 
Published: June 2019

Abstract
Glucose, fructose, and sucrose are important carbohydrates in Western diets with particular sweetness intensity and metabolisms. No study has compared their cerebral detection and their taste perception. Gustatory evoked potentials (GEPs), taste detection thresholds, intensity perception, and pleasantness were compared in response to glucose, fructose, and sucrose solutions at similar sweetness intensities and at identical molar concentrations. Twenty-three healthy subjects were randomly stimulated with 3 solutions of similar sweetness intensity (0.75 M of glucose, 0.47 M of fructose and 0.29 M of sucrose – sit. A), and with an identical molar concentration (0.29 M – sit. B). GEPs were recorded at gustatory cortex areas. Intensity perception and hedonic values of each solution were evaluated as were gustatory thresholds of the solutions. No significant difference was observed concerning the GEP characteristics of the solutions according to their sweetness intensities (sit. A) or their molar concentration (sit. B). In sit. A, the 3 solutions were perceived to have similar intensities and induced similar hedonic sensations. In sit. B, the glucose solution was perceived to be less intense and pleasant than the fructose and the sucrose solutions (P &lt; 0.001) and the fructose solution was perceived to be less intense and pleasant than the sucrose (P &lt; 0.001). Since GEP recordings were similar for glucose, fructose, and sucrose solutions whatever the concentrations, activation of same taste receptor induces similar cortical activation, even when the solutions were perceived differently. Sweet taste perception seems to be encoded by a complex chemical cerebral neuronal network.
Recent trends in molecular diagnostics of yeast infections: from PCR to NGS
Authors:   Consortium OPATHY, Arastehfar A , Boekhout T , Butler G , De Cesare G Buda , Dolk E , Gabaldón T , Hafez A , Hube B , Hagen F , Hovhannisyan H , Iracane E , Kostrzewa M , Lackner M , Lass-Flörl C , Llorens C , Mixão V , Munro C , Oliveira-Pacheco J , Pekmezovic M , Pérez-Hansen A , Sanchez A Rodriguez , Sauer F M , Sparbier K , Stavrou A A , Vaneechoutte M , Vatanshenassan M , Gabaldón Toni 
Published: May 2019

ABSTRACTThe incidence of opportunistic yeast infections in humans has been increasing over recent years. These infections are difficult to treat and diagnose, in part due to the large number and broad diversity of species that can underlie the infection. In addition, resistance to one or several antifungal drugs in infecting strains is increasingly being reported, severely limiting therapeutic options and showcasing the need for rapid detection of the infecting agent and its drug susceptibility profile. Current methods for species and resistance identification lack satisfactory sensitivity and specificity, and often require prior culturing of the infecting agent, which delays diagnosis. Recently developed high-throughput technologies such as next generation sequencing or proteomics are opening completely new avenues for more sensitive, accurate and fast diagnosis of yeast pathogens. These approaches are the focus of intensive research, but translation into the clinics requires overcoming important challenges. In this review, we provide an overview of existing and recently emerged approaches that can be used in the identification of yeast pathogens and their drug resistance profiles. Throughout the text we highlight the advantages and disadvantages of each methodology and discuss the most promising developments in their path from bench to bedside.
The Insulin Signaling Substrate Chico and the Ecdysone Response Element Broad Both Regulate Growth of the Head Horns in the Asian Rhinoceros Beetle, Trypoxylus dichotomus
Authors: Lavine Mark D , Gotoh Hiroki , Hayes Abigail , Corley Lavine Laura 
Published: May 2019

AbstractMales of the Asian rhinoceros beetle, Trypoxylus dichotomus, possess exaggerated head and thoracic horns that scale dramatically out of proportion to body size. While RNAi-mediated knockdowns of the insulin receptor suggest that the insulin signaling pathway regulates nutrition-dependent growth including exaggerated horns, the genes that regulate disproportionate growth have yet to be identified. We used RNAi-mediated knockdown of several genes to investigate their potential role in growth and scaling of the sexually dimorphic, exaggerated head horns of T. dichotomus. Knockdown of the insulin signaling substrate chico and the ecdysone response element broad caused significant decreases in head horn length, while having no or minimal effects on other structures such as elytra and tibiae. However, scaling of horns to body size was not affected by either knockdown. In addition, knockdown of phosphatase and tensin homolog, a negative regulator of the insulin signaling pathway, had no significant effects on any trait. Our results do not identify any candidate genes that may specifically mediate the allometric aspect of horn growth, but they do confirm the insulin signaling pathway as a mediator of conditional trait expression, and importantly implicate the ecdysone signaling pathway, possibly in conjunction with insulin signaling, as an additional mediator of horn growth.
Protection From Lethal Lassa Disease Can Be Achieved Both Before and After Virus Exposure by Administration of Single-Cycle Replicating Lassa Virus Replicon Particles
Authors: Kainulainen Markus H , Spengler Jessica R , Welch Stephen R , Coleman-McCray JoAnn D , Harmon Jessica R , Scholte Florine E M , Goldsmith Cynthia S , Nichol Stuart T , Albariño César G , Spiropoulou Christina F 
Published: May 2019

AbstractLassa fever is a frequently severe human disease that is endemic to several countries in West Africa. To date, no licensed vaccines are available to prevent Lassa virus (LASV) infection, even though Lassa fever is thought to be an important disease contributing to mortality and both acute and chronic morbidity. We have previously described a vaccine candidate composed of single-cycle LASV replicon particles (VRPs) and a stable cell line for their production. Here, we refine the genetic composition of the VRPs and demonstrate the ability to reproducibly purify them with high yields. Studies in the guinea pig model confirm efficacy of the vaccine candidate, demonstrate that single-cycle replication is necessary for complete protection by the VRP vaccine, and show that postexposure vaccination can confer protection from lethal outcome.
Genome-wide association analysis for porcine reproductive and respiratory syndrome virus susceptibility traits in two genetic populations of pigs1
Authors: Walker Lianna R , Jobman Erin E , Sutton Kylee M , Wittler J’Nan , Johnson Rodger K , Ciobanu Daniel C 
Published: May 2019

Abstract
Porcine reproductive and respiratory syndrome virus (PRRSV) is an economically important pathogen that continues to threaten swine industry sustainability. The complexity and high genetic diversity of PRRSV has prevented vaccines from conferring adequate protection against disease outbreaks. Genome-wide association analyses of PRRSV experimentally infected pigs representing two genetic lines (n = 174 to 176) revealed two major genomic regions accounting for ~1.2% of the genetic variation in PRRSV-specific antibody level in serum or lung. The major region for serum antibody was mapped to SSC7 near the SLAII complex, which has also been implicated in susceptibility to other swine viral pathogens. Haplotype substitution analysis uncovered potential DQB1 haplotypes associated with divergent effects. A novel major region for lung antibody was mapped to the proximal end of SSC17 with the top SNP overlapping two genes, PRAG1 and LONRF1. Sequencing LONRF1 uncovered polymorphisms within the coding region that may play a role in regulating PRRSV-specific antibody production in lung tissue following PRRSV infection. These data implicate novel host genomic regions (SSC17) that influence PRRSV-specific immune response as well as a common region (SSC7) potentially involved in susceptibility to multiple viral pathogens.
How Pedro Quiñonez Lost His Soul: Suicide, Routine Violence, and State Formation in Nineteenth-Century Paraguay
Authors: Huner Michael Kenneth 
Published: May 2019

Abstract
This article uses judicial records of suicide cases from mid-nineteenth-century Paraguay to explore how inhabitants of the country experienced postcolonial state formation. The Republic of Paraguay, founded in 1813, was a marginal, autonomous state in South America, forged from a former Spanish colonial frontier province and dominated by autocratic regimes until its near destruction in the Triple Alliance War (1864–1870). Conventional historical wisdom suggests that unlike other countries in Latin America at the time, matters of nationhood and state sovereignty in Paraguay were largely resolved. Stable autocratic regimes, muted social inequalities among a common peasantry, and ethnolinguistic bonds born of the indigenous-origin vernacular Guaraní allegedly provided for this resolution. However, this article disrupts such received wisdom and finds that state formation in Paraguay by the mid-nineteenth century was still very much an unsettled, ongoing affair, especially in the intensely local contexts of the countryside. Its narrative follows the 1865 suicide of the retired militia officer Pedro Quiñonez and what a local judge did and wrote to condemn his soul. In fact, this and dozens of other cases of suicide reviewed in the article reveal a social world of servitude, racialized denigration, and routine violence that were part and parcel of everyday people’s incorporation into ñane retã—the Guaraní-language expression for the nation-state—and the consequent slippery ambivalence that Pedro and others manifested in their relation to this polity.
Environmental effects on the UV upturn in local clusters of galaxies
Authors: Ali Sadman S , Bremer Malcolm N , Phillipps Steven , De Propris Roberto 
Published: May 2019

ABSTRACT
We explore the dependence of ultraviolet (UV) upturn colours in early-type cluster galaxies on the properties of their parent clusters (such as velocity dispersion and X-ray luminosity) and on the positions and kinematics of galaxies within them. We use a sample of 24 nearby clusters with highly complete spectroscopy and optical/infrared data to select a suitable sample of red-sequence galaxies, whose far-ultraviolet and NUV magnitudes we measure from archival GALEX data. Our results show that the UV upturn colour has no dependence on cluster properties and has the same range in all clusters. There is also no dependence on the projected position within clusters or on line-of-sight velocity. Therefore, our conclusion is that the UV upturn phenomenon is an intrinsic feature of cluster early-type galaxies, irrespective of their cluster environment.
Ambipolar diffusion in large Prandtl number turbulence
Authors: Brandenburg Axel 
Published: May 2019

ABSTRACT
We study the effects of ambipolar diffusion (AD) on hydromagnetic turbulence. We consider the regime of large magnetic Prandtl number, relevant to the interstellar medium. In most of the cases, we use the single-fluid approximation where the drift velocity between charged and neutral particles is proportional to the Lorentz force. In two cases we also compare with the corresponding two-fluid model, where ionization and recombination are included in the continuity and momentum equations for the neutral and charged species. The magnetic field properties are found to be well represented by the single-fluid approximation. We quantify the effects of AD on total and spectral kinetic and magnetic energies, the ohmic and AD dissipation rates, the statistics of the magnetic field, the current density, and the linear polarization as measured by the rotationally invariant E and B mode polarizations. We show that the kurtosis of the magnetic field decreases with increasing AD. The E mode polarization changes its skewness from positive values for small AD to negative ones for large AD. Even when AD is weak, changes in AD have a marked effect on the skewness and kurtosis of E, and only a weak effect on those of B. These results open the possibility of employing E and B mode polarizations as diagnostic tools for characterizing turbulent properties of the interstellar medium.
Bayesian cross validation for gravitational-wave searches in pulsar-timing array data
Authors: Wang Haochen , Taylor Stephen R , Vallisneri Michele 
Published: June 2019

ABSTRACT
Gravitational-wave data analysis demands sophisticated statistical noise models in a bid to extract highly obscured signals from data. In Bayesian model comparison, we choose among a landscape of models by comparing their marginal likelihoods. However, this computation is numerically fraught and can be sensitive to arbitrary choices in the specification of parameter priors. In Bayesian cross validation, we characterize the fit and predictive power of a model by computing the Bayesian posterior of its parameters in a training data set, and then use that posterior to compute the averaged likelihood of a different testing data set. The resulting cross-validation scores are straightforward to compute; they are insensitive to prior tuning; and they penalize unnecessarily complex models that overfit the training data at the expense of predictive performance. In this article, we discuss cross validation in the context of pulsar-timing-array data analysis, and we exemplify its application to simulated pulsar data (where it successfully selects the correct spectral index of a stochastic gravitational-wave background), and to a pulsar data set from the NANOGrav 11-yr release (where it convincingly favours a model that represents a transient feature in the interstellar medium). We argue that cross validation offers a promising alternative to Bayesian model comparison, and we discuss its use for gravitational-wave detection, by selecting or refuting models that include a gravitational-wave component.
Adolescents’ Experiences of Music Therapy in an Inpatient Crisis Stabilization Unit
Authors: Rosado Amanda 
Published: May 2019

Abstract
Fourteen adolescents on an inpatient crisis stabilization unit were interviewed about their experiences of group music therapy in order to understand how sessions impacted their mental health recovery. Using analytic procedures consistent with qualitative content analysis, 13 preliminary themes captured their session experiences. From these themes, 4 central concepts emerged: (1) music therapy affirms participants’ strengths, (2) music therapy affirms coping resources, (3) music therapy integrates cognitive and affective processes, and (4) music therapy provides continuity of experiences. Each theme and concept is defined, along with implications for clinical implications and future research.
Metal sensing and regulation of adaptive responses to manganese limitation by MtsR is critical for group A streptococcus virulence
Authors: Do Hackwon , Makthal Nishanth , Chandrangsu Pete , Olsen Randall J , Helmann John D , Musser James M , Kumaraswami Muthiah 
Published: June 2019

Abstract
Pathogenic bacteria encounter host-imposed manganese (Mn) limitation during infection. Herein we report that in the human pathogen Streptococcus pyogenes, the adaptive response to Mn limitation is controlled by a DtxR family metalloregulator, MtsR. Genes upregulated by MtsR during Mn limitation include Mn (mtsABC) and Fe acquisition systems (sia operon), and a metal-independent DNA synthesis enzyme (nrdFEI.2). To elucidate the mechanism of metal sensing and gene regulation by MtsR, we determined the crystal structure of MtsR. MtsR employs two Mn-sensing sites to monitor metal availability, and metal occupancy at each site influences MtsR regulatory activity. The site 1 acts as the primary Mn sensing site, and loss of metal at site 1 causes robust upregulation of mtsABC. The vacant site 2 causes partial induction of mtsABC, indicating that site 2 functions as secondary Mn sensing site. Furthermore, we show that the C-terminal FeoA domains of adjacent dimers participate in the oligomerization of MtsR on DNA, and multimerization is critical for MtsR regulatory activity. Finally, the mtsR mutant strains defective in metal sensing and oligomerization are attenuated for virulence in a mouse model of invasive infection, indicating that Mn sensing and gene regulation by MtsR are critical processes during S. pyogenes infection.
Diagnosis and Genotyping of Coxiella burnetii Endocarditis in a Patient with Prosthetic Pulmonary Valve Replacement Using Next-Generation Sequencing of Plasma Microbial Cell-Free DNA
Authors: Kondo Maiko , Dalai Sudeb C , Venkatasubrahmanyam Shivkumar , Eisenberg Nell , Robinson Brian D , Westblade Lars F , Marks Kristen M 
Published: June 2019

AbstractDetermining the causative etiology of culture-negative endocarditis can be challenging. We performed next-generation sequencing of plasma microbial cell-free DNA to facilitate rapid diagnosis and genotyping of Coxiella burnetii in a patient with culture-negative endocarditis of a prosthetic pulmonary valve, enabling early targeted treatment prior to valve replacement surgery.
The ‘Policy’ That Invalidates Testamentary Conditions
Authors: Jensen Darryn 
Published: May 2019

Abstract
Whenever one person’s conferral of a benefit on another is subject to a condition that the conferee not be married to a particular person or to a member of a specified class of persons, the question of whether the condition is enforceable is said to be a question of ‘public policy’. This ‘policy’ question is fundamentally a question of whether enforcing the condition contradicts or undermines any of the norms concerning the conduct of married persons to which the law is committed. It is not a question of whether enforcing or not enforcing the condition in the particular case would advance a more general social goal of encouraging marriage and discouraging divorce. A principle of preserving the coherence of the law—that the law ‘refuses to give by its right hand what it takes away by its left hand’—is the animating principle of both the marriage condition cases and the illegality cases.
Improving Zinc and Iron Accumulation in Maize Grains Using the Zinc and Iron Transporter ZmZIP5
Authors: Li Suzhen , Liu Xiaoqing , Zhou Xiaojin , Li Ye , Yang Wenzhu , Chen Rumei 
Published: May 2019

Abstract
Zinc (Zn) and iron (Fe) are essential micronutrients for plant growth. Thus, it is important to understand the mechanisms of uptake, transport and accumulation of these micronutrients in maize to improve crop nutritional quality. Members of the zinc-regulated transporters, iron-regulated transporter-like protein (ZIP) family are responsible for the uptake and transport of divalent metal ions in plant. Previously, we showed that ZmZIP5 functionally complemented the Zn uptake double mutant zrt1zrt2, Fe-uptake double mutant fet3fet4 in yeast. In our β-glucuronidase (GUS) assay, the germinated seeds, young sheaths, and stems of ZmZIP5-promoter-GUS transgenic plants were stained. We generated and compared two maize lines for this study: Ubi-ZmZIP5, in which ZmZIP5 was constitutively overexpressed, and ZmZIP5i, a RNAi line. At the seedling stage, high levels of Zn and Fe were found in the roots and shoots of Ubi-ZmZIP5 plants, whereas low levels were found in the ZmZIP5i plants. Zn and Fe contents decreased in the seeds of Ubi-ZmZIP5 plants and remained unchanged in the seeds of ZmZIP5i plants. The seeds of Leg-ZmZIP5 plants, in which ZmZIP5 overexpression is specific to the endosperm, had higher levels of Zn and Fe. Our results imply that ZmZIP5 may play a role in Zn and Fe uptake and root-to-shoot translocation. Endosperm-specific ZmZIP5 overexpression could be useful for Zn and Fe biofortification of cereal grains.
Exploring the Growth in Police Engagement with Those Who Are Mentally Ill and the Developing Use of the Mental Capacity Act as an Alternative to Section 136 of the Mental Health Act
Authors: Thomas Allyn , Forrester-Jones Rachel , Hunt Peter 
Published: May 2019

Abstract
Despite efforts of Parliament, the Home Office, police forces and health practitioners, the number of people detained under Section 136 of the Mental Health Act 1983 continues to rise. By analyzing quantitative data from Hampshire Constabulary, this study describes in detail police engagement with the mentally ill. Findings showed how people in mental crisis were increasingly detained by the ambulance service using the Mental Capacity Act and taken to A&E Departments. Nationally, police officers were also found to be increasingly taking Section 136 detainees to A&E. The majority of people contacting Hampshire displayed delusions which were unlikely to result in a police response and may disproportionately account for the overall growth in contact with the police. The data suggest that ‘Triage’ schemes alone will not be effective in reducing detentions. Hampshire’s partnership and discretionary approach is successful in reducing detentions. Recommendations for policy, practice, and further research are provided.
“Who Can We Tell Survivors to Call?” The Institutionalization of Criminal-Legal Interventions in a Domestic Violence Organization
Authors: Weiss Benjamin R 
Published: May 2019

AbstractAlthough criminal-legal interventions became accepted as the best response to domestic violence early in the battered women’s movement, recent literature suggests that such interventions are often ineffective in reducing rates of violence. Despite this evidence, domestic violence advocates still emphasize criminal-legal interventions over alternatives when working with victims of violence. The author spent thirteen months observing domestic violence advocates in a feminist nonprofit organization to learn how the paradoxical reliance on criminal-legal interventions is reproduced. The paper identifies three mechanisms. First, information on criminal-legal interventions is emphasized more than other interventions in advocate training. Second, nonprofit staff present extreme examples of abuse and their associated criminal-legal interventions while training advocates rather than describing the types of abuse more typically brought to the organization. Finally, staff establish protocols for handling advocates’ discontent with criminal-legal interventions which makes further collaboration between volunteers and the police possible. Together, these mechanisms reproduce criminal-legal interventions by limiting advocates’ knowledge of the variety of alternative interventions. Practically, this work suggests several ways for advocates to destabilize ineffective organizational practices. Theoretically, the author shows that normative institutions are reproduced not just through socialization and coercion, but also through a lack of alternatives.