bluuebunny/crossref_metadata_2025_split huggingface dataset:
N-methyl-D-aspartate receptor activation is downstream coupled to pannexin 1 opening by Src kinase in dorsal horn neurons: an essential link for mechanical hyperalgesia in nerve-injured rats
Authors: Zepeda-Morales Katherine , Bravo David , Aránguiz-Barrera Jonathan , Ampuero Estibaliz , Renard Georgina M. , Pelissier Teresa , Hernández Alejandro , Retamal Jeffri S. , Constandil Luis 
Published: December 2024

Abstract
A well-recognized molecular entity involved in pain-related neuroplasticity is the N-methyl-D-aspartate receptor (NMDAR), which is crucial for developing chronic pain. Likewise, the pannexin 1 (Panx1) channel has been described as necessary for initiating and maintaining neuropathic pain, driving nociceptive signals dependent on spinal NMDAR through several possible mechanisms. Through behavioral, pharmacological, and molecular approaches, our study in male rats has revealed several key findings: (1) neurons located in spinal cord laminae I and II express functional Panx1 channels in both neuropathic and sham rats. These channels can open (indicated by YOPRO-1 uptake) through the stimulation of NMDARs with intrathecal NMDA; (2) intrathecal NMDA leads to increased expression of pSrc and pPanx1 in dorsal horn neurons. This elevation exacerbates existing mechanical hyperalgesia in nerve-injured rats; (3) inhibition of Src with intrathecal PP2 or blockade of Panx1 with intrathecal 10Panx effectively mitigates NMDA-induced effects and reduces the spontaneous mechanical hyperalgesia of nerve-injured rats. Notably, while 10Panx successfully alleviates hyperalgesia, it does not alter pSrc expression; and (4) NMDA-stimulated YOPRO-1 uptake in neurons of laminae I-II of spinal cord slices were prevented by the NMDAR antagonist D-AP5, the Src inhibitor PP2 (but not PP3), as well as with the 10Panx and carbenoxolone. Therefore, NMDAR activation in dorsal horn neurons triggers an NMDAR-Src-Panx1 signaling pathway, where Panx1 acts as an enhancing effector in neuropathic pain. This implies that disrupting the NMDAR-Panx1 communication (eg, through Src inhibitors and/or Panx1 blockers) may offer a valuable strategy for managing some forms of chronic pain.
Evaluation of a Cohort-Based Healthy Aging With HIV Wellness Pilot Intervention: “People Aging and Thriving With HIV” in Colorado
Authors: Burk-Leaver Erin , Zivalich Christopher , Sunshine Justine , Lowry Christopher A. , Erlandson Kristine M. 
Published: December 2024

Abstract
The aging population of people living with HIV (PLWH) face unique health and social challenges. We piloted a community-based wellness program, People Aging and Thriving with HIV (PATH2Wellness), aiming to improve health outcomes through physical activity, nutritional counseling, and social support within Colorado Health Network, a statewide AIDS service organization. Participants ages 50+ were recruited into three cohorts between September 2020 and December 2021. Weekly step counts and psychosocial measures were collected. Overall, while there were no statistically significant improvements in step count or secondary outcomes (including depression, social connectedness, self-reported physical function, and quality of life [all p ≥ .55]), participants noted a high level of satisfaction. Ongoing feedback led to modifications for program improvement; as such, PATH2Wellness shows promise in improving mental health and social connectedness, and findings underscore the importance of tailored programs for older PLWH, suggesting avenues for research and program development.
Sleep Disturbances and Risk of Sports Injury Among Collegiate Student-Athletes
Authors: Messman Brett A. , Petrie Kyla A. , Moore E. Whitney G. , Petrie Trent A. 
Published: December 2024

Objective:
The purpose of this study was to examine the relationship between collegiate student-athletes’ sleep and injury status.

Design:
This study was a secondary analysis of observational survey data of student-athletes collected across 2 time periods, October 2020 and January 2021.

Setting:
Data were collected as part of an ongoing national (USA) longitudinal survey investigating student-athletes’ well-being in the aftermath of the COVID-19 pandemic.

Participants:
Participants were made-up of a subsample of participants from the parent study. Data from 89 exact matched pairs (across 5 demographics) of injured and noninjured student-athletes (68.5% NCAA Division I, 82.0% women) were assessed.

Independent Variables:
Student-athletes’ sleep disturbance levels were the primary independent variable of interest. Analytic models also covaried for gender, racial, and ethnic identities, sport in-seasons, and previous injury status.

Main Outcome Measures:
Participant endorsed injury status was the main outcome measure of interest. Hypotheses were formulated during the data collection of the parent study.

Results:
Student-athletes injured in January 2021 reported higher average sleep disturbances in both October 2020 and January 2021 than noninjured student-athletes but not student-athletes injured in October. In addition, October 2020 sleep disturbances were a significant predictor (P = 0.007) of injury, such that odds of student-athletes being injured increased 1.07 times for each unit increase in their sleep disturbances controlling for their identities, sport in-season, and previous injury status.

Conclusions:
Findings signal support for the screening and targeting of student-athletes’ sleep problems to reduce potential occurrences of sports-related injuries in student-athletes.
The Relationship Between Psychopathology, Personality Traits, and a History of Traumatic Brain Injury
Authors: Sarwer David B. , Spitzer Jacqueline C. , Wu Jingwei , LaGrotte Caitlin , Griffiths Caitlin , Ashare Rebecca L. , McDevitt Jane , Langford Dianne , Ramirez Servio H. 
Published: December 2024

Objectives:
To determine whether history of traumatic brain injury (TBI) is associated with psychopathology, and, in particular, substance use disorder (SUD) in young adults with a history of athletic participation.

Design:
Observational study.

Setting:
University in the northeastern United States.

Participants:
A large (n = 534) and mixed sex (57% female) sample of varsity and club sport athletes older than 18 years.

Interventions:
None.

Main Outcome Measure:
The Ohio State University Traumatic Brain Injury ID assessment and the Structured Clinical Interview for the diagnostic and statistical manual of mental disorders, fifth edition.

Results:
Twenty-five percent of participants reported no history of head injuries, 32% reported 1 head injury, and 43% reported more than 1 head injury. Thirty-eight percent of participants had been diagnosed with 1 or more concussions. Twenty-eight percent of participants had a current psychiatric diagnosis and 49% had at least 1 lifetime diagnosis. Head injuries and concussions were significantly associated with the presence of lifetime psychiatric diagnosis and symptoms or diagnosis of alcohol or SUD. More head injuries were associated with symptoms of post-traumatic stress disorder.

Conclusions:
Higher number of self-reported head injuries and diagnosed concussions were associated with a lifetime history of psychopathology, and specifically, with alcohol and substance use disorders. These relationships are similar to those seen in the general population of adults. The relationships also raise several interesting questions about recovery from head injuries in young adult athletes.
Proinflammatory Cytokine Profiles in Menière's Disease and Vestibular Migraine
Authors: Monaghan Neil P. , Shah Sunny , Keith Brian A. , Nguyen Shaun A. , Newton Danforth A. , Baatz John E. , Wagner Carol L. , Rizk Habib G. 
Published: December 2024

Objective
To evaluate the levels of inflammatory cytokines and symptom survey scores in patients diagnosed with Menière's disease or vestibular migraine from a single center by a single neurotologist compared to control subjects with no history of dizziness or migraine.

Study Design
Cross-sectional pilot study.

Setting
Single-center tertiary referral center in Charleston, SC.

Patients
Patients were recruited from the neurotology clinic at a tertiary referral center. Patients with definite Menière's disease or definite vestibular migraine as defined by the Barany consensus criteria were eligible. Control subjects presented to clinic without dizziness, vertigo, fluctuating hearing loss, or a history of migraine.

Main Outcome Measures
Questionnaire scores include DHI, SF-20, CFQ, PHQ-9, PSWQ, GAD-7, NVI, DCS, VM-PATHI, and MD-POSI. Circulating and in vitro levels of cytokines include ENA-78, GROα, IFN-α2a, IFN-γ, IL-10, IL-1α, IL-1β, IL-1RA, IL-2, IL-4, IL-5, IL-6, IL-8, MCP-1, MCP-2, MDC, MIP-1α, MIP-1β, and TNF-α. Cytokine levels were compared with effect size analysis.

Results
There were 20 Menière's disease, 20 vestibular migraine, and 10 control patients enrolled in this study. Episode frequencies ranged from three per week to two to five per year in the MD group and daily to 1 every 2 to 3 months in the VM group. When patient-derived PBMC samples were compared to vestibular migraine, TNF-α (d = −0.427 [−0.879, 0.025]) and IFN-γ (d = −0.818 [−1.313, −0.323]) were found to be higher in Menière's disease, whereas ENA-78 (d = −0.652 [−1.361, 0.056]) was found to be lower. No differences were found when cytokines were measured following stimulation with LPS.

Conclusions
This pilot study suggests Menière's disease patients may have higher levels of TNF-α and IFN-γ and lower levels of ENA-78 than vestibular migraine patients when measured following in vitro release from patient-derived PBMC. Increased sample size, optimized blood draw timing, and more specific PBMC stimulation may help us further elucidate inflammatory pathways implicated in these disorders.
Cardiovascular Diseases and Sensorineural Hearing Loss—A Systematic Review of the Literature
Authors: Berezovsky Anna N. , Espahbodi Mana , LaPrade Samantha L. , Friedland David R. , Harris Michael S. 
Published: December 2024

Objectives
To assess the relationship of cardiovascular disease (CVD) and sensorineural hearing loss (SNHL).

Databases Reviewed
Ovid MEDLINE, Web of Science, Scopus, and Cochrane.

Methods
A systematic review was performed. Studies were identified using Ovid MEDLINE, Web of Science, Scopus, and Cochrane from 1946 to 2023. Per Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, abstracts were screened for primary review. Full texts were reviewed for secondary review.

Results
There were 3,440 unique abstracts screened. After primary review of abstracts and secondary full text review, 68 articles were included. The relationship between SNHL and the following CVDs, risk factors, and interventions were ascertained: coronary artery bypass graft (CABG) surgery, coronary artery disease (CAD), carotid stenosis (CS), myocardial infarction (MI), percutaneous coronary intervention (PCI), other arteriosclerosis, cerebral small vascular disease, cerebrovascular accident (CVA), transient ischemic attack (TIA), peripheral vascular disease (PVD), hyperlipidemia (HLD), hypertension (HTN), and diabetes mellitus (DM). CABG and CAD were consistently associated with SNHL. DM was associated with SNHL, but HTN and HLD were not consistently associated.

Conclusions
There is a complex relationship between CVDs and SNHL. CABG and CAD were consistently associated with SNHL. DM was associated with SNHL, but other CVD risk factors (HTN and HLD) did not appear to consistently correlate with SNHL, raising consideration that SNHL is a later finding in those with CVD.
Diagnosis of Death Using Neurological Criteria in Children: The U.K. Experience, 2015–2023*
Authors: Aziz Omer , Main Thomas B. N. , Hannon Faith R. , Fraser James I. 
Published: December 2024

OBJECTIVES:
In the United Kingdom, the starting point for diagnosing and confirming death using neurologic criteria (DNC) is when death is suspected to have occurred following devastating brain injury in patients on an ICU who remain deeply comatose, have absent brainstem reflexes and are apneic with their lungs mechanically ventilated but in whom circulation and other bodily functions persist. In this report, we describe the U.K. experience of diagnosing DNC in children.

DESIGN:
Retrospective review of data collected by the U.K. National Health Service Blood and Transplant Potential Donor Audit.

SETTING AND PATIENTS:
U.K. children from birth to younger than 18 years old with suspected DNC between April 1, 2015, and April 1, 2023.

INTERVENTIONS:
None.

MEASUREMENTS AND MAIN RESULTS:
A total of 824 children were suspected of DNC and 565 of 824 (68.6%) proceeded to have testing. Overall, 393 of 565 (69.5%) of the tested patients were cared for on a PICU, 164 of 565 (29%) on an adult ICU, three of 565 (0.5%) on a neonatal ICU, and the other five of 565 (1%) in other locations. After testing, 548 of 565 (97%) were confirmed as having died using neurologic criteria. During 2020 to 2023, we estimate that DNC occurred in 6.2% of all PICU deaths. Of 393 of 565 children undergoing testing on the PICU, 15 were younger than 2 months, 45 between 2 months to 1 year, and 31 between 1 and 2 years.

CONCLUSIONS:
During the 8 years, 2015–2023, out of 824 children younger than 18 years old suspected of being dead using neurologic criteria, close to one-in-three did not proceed to formal testing. In 2020–2023, DNC in U.K. PICUs accounted for 6.2% of all death. Last, in young children up to 2 years old, the average experience of diagnosing and confirming DNC across each of the 30 U.K. PICUs was one patient every other year.
User preference and patient benefits of a novel energy storing and return foot: A randomized, cross-over clinical trial
Authors: Maciejasz Pawel , Budny Tymoteusz , Sauer Michael , Umari Marina , Korber Jürgen , Ernst Jennifer , Altenburg Bjoern , Hahn Andreas , Braatz Frank 
Published: December 2024

Background:
Energy storing and return (ESAR) prosthetic feet often provide high energy return at the cost of increased foot height, compromised appearance, and reduced flexibility. An ESAR foot, Ottobock Evanto, with a novel compact design has been introduced (novel foot).

Objective:
To characterize user preference and benefits of a novel foot compared with the subject's previously worn (everyday foot) and comparator foot, Össur Pro-Flex XC.

Study Design:

Multicenter, randomized, nonblinded, cross-over trial (ClinicalTrials.gov, NCT05628064).

Methods:
Transtibial amputees using ESAR feet were eligible. Outcome measures included perceived mobility, the extent of meeting amputees' needs, pain intensity, EuroQol 5-Dimension 5-Level, Activities-specific Balance Confidence, pain interference, foot preference, and walking experience. The first 3 measures with the novel foot vs. the everyday and comparator feet were used for primary objectives.

Results:
Data from 20 participants were analyzed. For all primary outcomes, improved scores while wearing the novel foot were obtained. The change in perceived mobility vs. the everyday foot score was highly significant (P = 0.0007). The novel foot was the most preferred foot overall (55% participants) and for many activities, e.g., walking on inclines (85%) and over uneven terrain (70%). Participants confirmed that the novel foot made walking up slopes easier (95%), standing more flexible (85%), and heel strike more comfortable (85%) vs. their everyday feet.

Conclusions:
The novel foot offers advantages compared with the tested feet, including making walking on uneven terrain and slopes easier and more comfortable heel strike. Activities in which the novel foot would perform worse than the other feet have not been reported.
Predictors of frailty trajectories among people with HIV
Authors: Iriarte Evelyn , Smyth Heather L. , Schmiege Sarah , Tassiopoulos Katherine , Jankowski Catherine M. , Erlandson Kristine M. 
Published: December 2024

Objective:
This study aimed to estimate the latent frailty trajectories and identify corresponding predictors (sociodemographic, HIV-related, comorbidities, and behavioral) among a cohort of people with HIV (PWH).

Design:
Longitudinal observational study using latent class growth modeling.

Methods:
Nine hundred and seventy-six PWH aged 40 years and older with frailty measured from at least two visits within the ACTG HAILO cohort were included. Frailty components included weakness, physical activity, weight loss, exhaustion, and slowness. Latent class growth models were estimated to capture change in frailty over time; multinomial logistic regression was used to estimate associations between predictors and frailty trajectory class.

Results:
At baseline, participants were M = 51.5 years old (SD = 7.5), 81% male (n = 783), 48% White non-Hispanic (n = 461), and 20% Hispanic (n = 195). Latent class growth models identified three frailty trajectories: sustained robustness (n = 811; 83%), worsening frailty (n = 79; 8%), and frailty improvement (n = 86; 9%). Older age, race, sex at birth, select comorbidities (cardiovascular disease, depression, type 2 diabetes), and behavioral characteristics (physical activity, smoking, and alcohol) were associated with fluctuations in frailty trajectories over time (P < 0.05).

Conclusion:
Modifiable factors such as managing comorbidities and promoting physical activity present ideal opportunities for future interventions to prevent or slow the progression of frailty.
Solitary Scapula Metastasis From Intrahepatic Cholangiocarcinoma Detected by PET/CT
Authors: Xu Churun , Ren Pengcheng , Zhao Guoxing , Wei Lingge , Xie Peng 
Published: December 2024

Abstract
A 59-year-old man presented with a mass and pain in the left side of his back for 6 months. 18F-FDG PET/CT demonstrated increased FDG uptake in the inferior aspect of his left scapula and the left hepatic lobe, highly suggesting the possibility of hepatic malignancy with solitary scapula metastasis. Finally, solitary scapula metastasis from intrahepatic cholangiocarcinoma was confirmed.
Immediate postinjury extracorporeal carbon dioxide removal reduces ventilator requirements and mitigates acute respiratory distress syndrome in swine
Authors: Batchinsky Andriy I. , Roberts Teryn R. , Jordan Bryan S. , Beely Brendan M. , Wendorff Daniel S. , Necsoiu Corina , Cannon Jeremy W. , Chung Kevin K. , Cancio Leopoldo C. 
Published: December 2024

BACKGROUND
Awareness of ventilator-induced lung injury contributed to increased use of extracorporeal interventions, but not immediately after injury,before acute respiratory distress syndrome (ARDS) ensues. Our objective was to evaluate the role of venovenous extracorporeal carbon dioxide removal (ECCO2R) in management of mechanically ventilated swine with smoke inhalation injury and 40% body surface area burns.

METHODS
Yorkshire swine (n = 29, 43.2 ± 0.5 kg) underwent anesthesia, instrumentation, severe smoke inhalation, and 40% body surface area burns, followed by 72 hours of round-the-clock intensive care unit care with mechanical ventilation, fluids, pressors, bronchoscopic cast removal, computer tomography scans, and arterial blood assays. Within 1 hour after injury, animals received ECCO2R with either MiniLung (Xenios AG, Heilbronn, Germany; n = 10) or Hemolung (ALung Technologies, Pittsburgh, PA; n = 10), or no ECCO2R in injured controls (INJC, n = 12).

RESULTS
Immediate postinjury ECCO2R reduced minute ventilation (p < 0.001) and prevented ARDS in 37.5% of MiniLung and 11.1% of Hemolung animals. Time to ARDS (partial pressure of arterial oxygen to fraction of inspired oxygen ratio below 300) was shortest (14 ± 2.2 hours) in INJC, intermediate (21.6 ± 3.5 hours) in Hemolung (HEMO), and most delayed in MiniLung (31.1 ± 7.2 hours, p = 0.0121, log-rank test vs. INJC). Driving pressure was lower in MiniLung versus INJC (p < 0.0001) and HEMO versus INJC (p = 0.0005) at 48 hours. Extracorporeal CO2 removal reduced systemic levels of tumor necrosis factor α versus INJC.

CONCLUSION
In swine with severe smoke inhalation and burns, immediate postinjury ECCO2R reduced ventilator settings, delayed or prevented ARDS, and reduced its severity. Proactive early percutaneous ECCO2R initiation via simplified, purpose-built devices should be considered as a low-maintenance lung injury management approach with significant disease modifying clinical benefit potential.
Global population structure of a unicellular marine predator
Authors: Latorre Francisco , Jaillon Olivier , Sieracki Michael E. , Cruaud Corinne , Massana Ramon , Logares Ramiro 
Published: December 2024

ABSTRACTUnicellular predators, especially marine heterotrophic flagellates (HFs), are fundamental to marine food webs, facilitating the flow of nutrients from bacteria and the smallest primary producers to upper trophic levels. Comprehending their distributions and diversity is essential for understanding the functioning of marine ecosystems. The uncultured MArine STramenopiles clade 4 (MAST-4) constitutes a crucial HF member of the ocean microbiome with a predatory (bacterivorous) lifestyle. Temperature gradients have influenced the evolution and biogeography of MAST-4 species. Yet, similarly to most uncultured microbes, there is limited information on the population diversity and structure within MAST-4 species. This information is key for better comprehending the ecological roles and adaptations of different MAST-4 species to specific ocean niches. Here, we investigate the population diversity and structure on the global scale of the MAST-4 species A, B, C, and E by combining metagenomics and single-cell genomics data from theTara Oceansexpedition. We found substantial population divergence in the abundant MAST-4A and C species, while lower levels of divergence were observed in species B and E. Temperature and salinity were the main drivers structuring the populations within the four MAST-4 species. The analysis of positively selected gene clusters within each MAST-4 species revealed genomic areas likely the basis of population adaptation to different niches. Overall, our results contribute to a better comprehension of the population diversity and structure of four species belonging to an important group of unicellular predators, offering insights into their ecological roles and adaptations in the global ocean. The results also contribute to understanding microbial populations, a dimension of diversity that remains poorly known for most wild species and is pivotal in global change.