bluuebunny/crossref_metadata_2025_split huggingface dataset:
Endoprothetik
Authors: Perka Carsten , Janz Viktor 
Published: July 2018

ZusammenfassungDie Geschichte der Endoprothetik in den letzten 20 Jahren ist eine Erfolgsgeschichte. Mit modernen Implantaten sind heute nahezu alle Belastungen realisierbar.
Die steigende Anzahl an Endoprothesenimplantationen wird inzwischen allerdings auch als Problem diskutiert. Argumente sind das zu häufige Operieren und die
fehlende Nutzung der Alternativen, insbesondere der konservativen Behandlung. Zu den aktuellen Trends in der Endoprothetik zählt die immer stärkere
Konzentration endoprothetischer Versorgungen in Zentren bzw. großen Kliniken. Insbesondere in der Knieendoprothetik gehen höhere Fallzahlen mit einem besseren
Ergebnis einher. Durch Veränderungen rund um die Operation wurden die Verweildauern nach endoprothetischem Ersatz weltweit drastisch reduziert, weniger stark
aufgrund abrechnungsbedingter Aspekte in Deutschland. Insgesamt sind die Komplikationsraten, v.a. die aseptische Lockerung von Prothesen deutlich
zurückgegangen. Auch das Infektionsrisiko ist gesunken, stellt neben der Luxation und den periprothetischen Frakturen jedoch weiterhin eine Hauptursache für
eine Revisionsoperation dar.Aktuell können heute Standzeiten der Prothesen von 20 Jahren bei 90 % der Patienten angenommen werden. Eine Herausforderung in der Versorgung ergibt sich aus der
Reduktion der Erstattungsbeiträge durch Krankenkassen. Diese führt zu einem immer höheren Druck, Implantationen schneller und häufiger durchzuführen,
andererseits die verlustträchtige Behandlung von Komplikationen, v.a. Infektionen, in andere Krankhäuser zu verweisen.
Traditional Uses, Phytochemistry, and Antimicrobial Activities of Eugenia Species – A Review
Authors: de Souza Angela , de Oliveira Camila , de Oliveira Vinícius , Betim Fernando , Miguel Obdulio , Miguel Marilis 
Published: July 2018

AbstractAntimicrobial resistance is a critical health problem, and pathogens responsible for common infections have developed resistance to antimicrobials, posing a threat to global health and placing a huge burden on health services. During the past two decades, the search for new bioactive agents in nature has become extremely important for promoting health and in the development of more efficient antimicrobials. The genus Eugenia is one of the largest in the Myrtaceae family, comprising approximately 1000 species from Mexico to Argentina, with a few species distributed in Australia and Africa. Eugenia species are used in folk medicine, with antidiabetic, antirheumatic, antipyretic, anti-inflammatory, antidiarrheal, antifungal, and antibacterial properties. This study systematically reviews the Eugenia species to compile the phytochemical composition and antimicrobial effects. In addition, we provide information regarding the traditional uses and cytotoxic activity of Eugenia species. We conducted a systematic literature search of specialized databases (Web of Science, Scielo, Lilacs, Pubmed, Science Direct, Scopus) and selected articles published between 1973 and 2015 using Eugenia and antimicrobial activity, Eugenia and toxicity, and Eugenia and chemical composition as key words. Ninety-three studies were included, and the phytochemical analyses from these studies show that Eugenia species are a rich source of flavonoids, tannins, triterpenes, and sesquiterpenes. Chemical constituents play an apparent role in the antimicrobial effects and reinforce the known antimicrobial potential of the Eugenia genus. It is worth mentioning that some Eugenia species cause significant cytotoxicity.
Differential Modulation of Gene Expression Encoding Hepatic and Renal Xenobiotic Metabolizing Enzymes by an Aspalathin-Enriched Rooibos Extract and Aspalathin
Authors: Abrahams Sameega , Samodien Sedicka , Lilly Mariska , Joubert Elizabeth , Gelderblom Wentzel 
Published: July 2018

AbstractModulation of the expression of hepatic and renal genes encoding xenobiotic metabolizing enzymes by an aspalathin-enriched green rooibos (Aspalathus linearis) extract (GRE) was investigated in the liver and kidneys of F344 rats following dietary exposure of 28 d, as well as selected xenobiotic metabolizing genes in rat primary hepatocytes. In the liver, GRE upregulated genes (p < 0.05) encoding aldehyde dehydrogenase, glucose phosphate isomerase, and cytochrome P450 while 17β-hydroxysteroid dehydrogenase 2 (Hsd17β2) was downregulated. In primary hepatocytes, GRE lacked any effect, while aspalathin downregulated Hsd17β2, mimicking the effect of GRE in vivo, and upregulated catechol-O-methyl transferase and marginally (p < 0.1) cytochrome P450 2e1. In the kidneys, GRE upregulated (p < 0.05) genes encoding the phase II xenobiotic metabolism enzymes, glutathione-S-transferase mµ and microsomal glutathione-S-transferase, while downregulating genes encoding the ATP binding cassette transporter, cytochrome P450, gamma glutamyltransferase 1, and N-acetyltransferase 1. Differential modulation of the expression of xenobiotic metabolizing genes in vivo and in vitro by GRE is dose-related, duration of exposure, the tissue type, and interactions between specific polyphenol and/or combinations thereof. Aspalathin is likely to be responsible for the downregulation of estradiol and testosterone catabolism by GRE in the liver. The differential gene expression by GRE in the liver and kidneys could, depending on the duration exposure and dose utilized, determine the safe use of such an extract in humans for specific health and/or disease outcomes.
Functional Pool of Cyclic Adenosine 3',5'-Monophosphate in Rabbit Platelets
Authors: Hashimoto Shuichi G 
Published: July 2018

SummaryAccumulation of the newly formed 14C-cyclic adenosine 3',5’-monophosphate (cyclic AMP) was found in the P1 (1.0) fraction, i. e. a platelet plasma membrane fraction which was obtained from 14C-adenine-labeled platelets. On the other hand, total cyclic AMP as determined simultaneously was located mainly in the platelet soluble fraction. Furthermore, the highest value of the cyclic AMP-binding capacity was found in the P1 (1.0) fraction. The cyclic AMP-binding activity of platelet membranes was attributed to two proteins with molecular weights of approximately 48,000 and 68,000.The treatment of 14C-adenine-prelabeled platelets with thrombin (1 unit per ml) led to about 40% decrease in the newly formed 14C-cyclic AMP level and 18% reduction of 14C-adenosine triphosphate level in whole platelets within 10 sec. On the other hand, the 14C-cyclic AMP level in the P, fraction decreased by about 80% of the control value while the total cyclic AMP in this fraction was almost unchanged. This rapid and striking fall in the membrane 14C-cyclic AMP level could be correlated with the more than 2fold stimulation of the membrane-bound cyclic AMP phosphodiesterase, together with the more than 20% inhibition of both the cyclic AMP-binding capacity and the adenyl cyclase in platelet membranes by thrombin treatment. These observations suggest the possibility that functional pool of cyclic AMP related to thrombin-induced aggregation is located in rabbit platelet plasma membrane.
Albumin, Fibrinogen, Prothrombin and Antithrombin III Variations in Blood, Urines and Liver in Rat Nephrotic Syndrome (Heymann Nephritis)
Authors: Girot R , Jaubert F , Leon M , Bellon B , Aiach M , Josso F , Lepelletier O , Beguin S , Monnet J-P 
Published: July 2018

SummaryAlbumin, fibrinogen, prothrombin and antithrombin in (AT ID) variations have been studied in blood, urines and liver during an experimental nephrotic syndrome in rats (Heymann nephritis). A quantitative morphometric study (light microscopy) has been performed in the liver using an immunocytochemical technique – (PAP) method – to evaluate the protein synthesis by the number of protein-containing hepatocytes. Some sections were also studied by electron microscopy. The nephrotic animals were compared with control rats. In the blood of nephrotic rats, fibrinogen and prothrombin concentrations were increased and albumin and AT III concentrations were decreased. In the urines of nephrotic rats, albumin, prothrombin and AT III were lost, but no fibrinogen. The morphometric study in the liver has shown a significantly higher number of fibrinogen and prothrombin containing hepatocytes in nephrotic rats than in controls, suggesting an increased synthesis of these proteins; no change was observed concerning albumin and AT III between nephrotic and control animals. In electron microscopy, albumin was demonstrated in Golgi apparatus, proving that the peroxidase-positive cells are related to protein synthesis. These results show that the mechanisms of regulation of the protein synthesis during nephrotic syndrome are different from one protein to another and, particularly, that their blood level is not the only regulating factor for their synthesis.
The Inhibition by Plasma of Urokinase and Tissue Activator-Induced Fibrinolysis in Pregnancy and the Puerperium
Authors: Walker J E , Gow L , Campbell D M , Ogston D 
Published: July 2018

SummaryThe activity of urokinase and tissue activator on fibrin plates was inhibited by plasma from women in the third trimester of pregnancy to a greater extent than by non-pregnant plasma. Pregnancy also inhibited the amidolytic activity of urokinase. The high molecular weight fractions of pregnancy plasma gel filtered on Sephadex G-200 showed comparable inhibitory activity against urokinase as fractions for non-pregnant plasma; in contrast with non-pregnant plasma, the lower molecular weight fractions of pregnancy plasma were markedly inhibitory against urokinase. Plasma exposed to lysine-Sepharose to remove plasminogen and then fractionated on Sephadex G-100 provided a pattern of three areas of inhibition against tissue activator similar to that seen in non-pregnant plasma. The urokinase-inhibitory activity of lower molecular weight fractions of plasma separated on Sephadex G-200 fell within 1 hr of delivery and fell further over the following 18 to 30 hr.
The In Vitro Association of Antihemophilic Factor and von Willebrand Factor
Authors: Zucker M B , Soberano M E , Johnson A J , Fulton A J , Kowalski S , Adler M 
Published: July 2018

SummaryConsiderable evidence, including results of gel chromatography, indicates that antihemophilic factor (AHF; factor VIII: C) is associated with von Willebrand factor (vWF; factor VIIIR: RC or VTIIR: Ag) in citrated plasma. The present study was undertaken to determine whether these factors are also associated in plasma with a physiologic calcium ion concentration, or in an artificial medium using purified antihemophilic factor and plasma as a source of vWF. When fresh BaS04-treated native normal plasma was passed through a column of Sepharose CL-4B that was equilibrated and eluted with fresh BaS04-treated plasma from a patient with severe von Willebrand’s disease, the AHF and vWF activities were found in the void volume. Thus, AHF remains associated with vWF on gel chromatography in the presence of physiological concentrations of all plasma constituents except the vitamin-K-dependent clotting factors. On the other hand, when to 200,000 X purified “vWF-free” AHF was chromatographed in buffered 4% albumin with 2 mM CaCl2, virtually all of it appeared in the included volume of the column with an apparent molecular weight between that of fibrinogen or factor V (340,000) and gamma globulin (160,000). The combination of the “vWF-free” AHF with the vWF in plasma was studied by adding the AHF to BaS04-treated plasma from normal subjects or patients with severe hemophilia or von Willebrand’s disease and chromatographing the mixture. The AHF activity appeared in the void volume in an amount that was inversely related to the ratio of the AHF to vWF activity. Thus, with 1-12 U of AHF per unit of vWF, virtually all of the AHF eluted in the void volume, with 30 and 500 units of AHF per unit of vWF, only about 50% and 10% of the AHF, respectively, eluted in the void volume, and in the absence of vWF, none of the AHF activity eluted in the void volume.
Selective Inhibition of Thromboxane Synthetase with Dazoxiben - Basis of Its Inhibitory Effect on Platelet Adhesion
Authors: Menys V C , Davies J A 
Published: July 2018

SummaryPlatelet adhesion to rabbit aortic subendothelium or collagen-coated glass was quantitated in a rotating probe device by uptake of radio-labelled platelets. Under conditions in which aspirin had no effect, dazoxiben, a selective inhibitor of thromboxane synthetase, reduced platelet adhesion to aortic subendothelium by about 40% but did not affect adhesion to collagen-coated glass. Pre-treatment of aortic segments with 15-HPETE, a selective inhibitor of PGI2-synthetase, abolished the inhibitory effect of dazoxiben on adhesion. Concentrations of 6-oxo-PGFlα in the perfusate were raised in the presence of dazoxiben alone, and following addition of thrombin (10 units/ml) there was a 2-3 fold increase in concentration. Perfusion of damaged aorta with platelets labelled with (14C)-arachidonic acid in the presence of thrombin and dazoxiben resulted in the appearance of (14C)-labelled-6-oxo-PGFiα. Inhibition of thromboxane synthetase limits platelet adhesion probably by promoting vascular synthesis of PGI2 from endoperoxides liberated from adherent platelets, which subsequently promotes detachment of cells from the surface.
Factor VIII-Related Antigen in Cerebrospinal Fluid
Authors: Garcia V Vicente , Alberca I , Borrasca A Lopez 
Published: July 2018

SummaryA hundred and one samples of cerebrospinal fluid (CSF) were obtained from patients with bacterial meningitis (18), viral meningitis (9), lymphoproliferative disorders (33), 15 with meningeal infiltrations, multiple sclerosis (8), stroke (8) and 25 subjects with normal CSF. All samples were studied for VIIIR: Ag with specific and sensitive immunoradiometric assay (IRMA) and Laurell’s technique.Prothrombin and factor IX antigenic activities were investigated by Laurell’s technique. Simultaneously, plasma specimens from ten patients with bacterial meningitis were evaluated. Only a selective increase of VIIIR: Ag was demonstrated in CSF from bacterial meningitis whereas prothrombin and factor IX were not detected. VIIIR: Ag plasma and CSF levels were uncorrelated. Similarly, no relationship could be established between the degree of elevation of VIIIR: Ag in the CSF and their protein concentration.These findings suggest that VIIIR :Ag elevation in CSF has diagnostic value for bacterial meningitis and that disruption of the blood-brain barrier is not responsible for their elevated levels. Accordingly, the presence of VIIIR :Ag in CSF may be an indication of endothelial damage in the choroid plexi.
Highly Efficient Induction of Human Platelet Aggregation in Heparinized Platelet-Rich Plasma by Diadenosine Triphosphate (Ap3A)
Authors: Lüthje Jürgen , Baringer Johann , Ogilvie Adaling 
Published: July 2018

SummaryDiadenosine triphosphate (Ap3A), which is a releasable dinucleotide of human platelets, induces platelet aggregation when added to heparinized platelet-rich plasma. The concentration dependence of the dinucleotide is similar to ADP. This finding is fully compatible with our recent report of the low potency of Ap3A in citrated platelet-rich plasma relative to ADP.The aggregatory effect of Ap3A in heparinized versus citrated plasma is reflected in the corresponding rates of Ap3A degradation. In citrated plasma, the hydrolysis of Ap3A is drastically slowed down because the hydrolase needs divalent metal ions. The results strongly support the assumption that the aggregatory effect of Ap3A is mediated by the enzymatic hydrolysis of Ap3A which generates ADP as the ultimate stimulus.
Acylated Derivatives of Streptokinase-Plasminogen Activator Complex as Thrombolytic Agents in a Dog Model of Aged Venous Thrombosis
Authors: Dupe R J , Green J , Smith R A G 
Published: July 2018

SummaryA procedure is decribed for the establishment of experimental clots, aged in vivo for 72 hr, in the jugular vein of beagle dogs. Two acylated derivatives of streptokinase-human (lys) plasminogen activator complex with greatly differing deacylation rates under physiological conditions were compared as thrombolytic agents in the model. These were BRL 26921 (deacylation halflife, 40 min) and BRL 33575 (deacylation half-life c. 17hr). The pharmacokinetic clearance rate of BRL 33575 from the circulation was studied and gave a clearance half-life of about 7 hr. BRL 33575 was found to be the superior agent in lysing 72 hr aged clots, being effective at a single bolus dose of 420 μg/kg or in three equal divided doses of 140 μg/kg given at 12 hr intervals. The single dose regime gave moderate systemic plasminogen activation, and the effect was significantly reduced with the divided dose regime. Infusion of freshly formed streptokinasehuman plasminogen activator complex at 420 μg/kg over 15 hr gave little thrombolysis despite marked systemic plasminogen activation.
The Involvement of Platelets and the Coronary Vasculature in Collagen-Induced Sudden Death in Rabbits
Authors: Mallarkey G , Smith G M 
Published: July 2018

SummaryThe mechanism of collagen-induced sudden death in rabbits was studied by measuring blood pressure (BP), heart rate, ECG, the continuous platelet count and the plasma levels of thromboxane B2 (TxB2) and 6-keto prostaglandin Fia (6-keto PGF1α). Death was preceded by myocardial ischaemia and a sharp fall in BP which occurred before any fall in platelet count was observed. The calcium entry blockers (CEBs), verapamil, nifedipine and PY 108-068 protected the rabbits from sudden death without any significant effect on the decrease in the platelet count or increase in plasma TxB2 levels. 6-keto PGF1α could not be detected in any plasma samples. Indomethacin and tri-sodium citrate also protected the rabbits but significantly reduced the fall in platelet count and plasma TxB2. In vitro studies on isolated aortae indicated that verapamil non-specifically inhibited vasoconstriction induced by KC1, adrenaline and U46619 (a thromboxane agonist). It is concluded that CEBs physiologically antagonize the vasoconstricting actions of platelet-derived substances and that it is coronary vasoconstriction that is primarily the cause of death.
Effects of Prostacyclin and of the Stable Prostacyclin Analogue ZK 36374 on Forearm Blood Flow and Blood Platelet Behaviour in Man
Authors: Cowley A J , Heptinstall S , Hampton J R 
Published: July 2018

SummaryPGI2 and ZK 36374 were each infused into volunteers and the effects on forearm blood flow and on platelet behaviour were determined. Infusions of PGI2 or ZK 36374 did not alter resting forearm blood flow but both agents reduced the extent of the vasoconstriction that occurred in response to cold. ZK 36374 appeared to be a much more potent inhibitor of platelet behaviour than PGI2 when blood was taken while the infusions were in progress, but the effects of both agents were no longer evident one hour after the infusions were terminated. There was an inverse relationship between the extent of cold-induced vasoconstriction and the concentration of sodium arachidonate that was needed to induce platelet aggregation for different individuals. Infusions of PGI2 affected both parameters equally but ZK 36374 had a greater effect on platelet behaviour than on blood flow. It is possible that very low doses of ZK 36374 would result in inhibition of platelet behaviour without producing adverse haemodynamic effects.
The Electrophoretic Mobility Heterogeneity of Human Platelet Subpopulations of Different Buoyant Densities
Authors: Jung Stephanie M , Tanoue Kenjiro , Yamazaki Hiroh 
Published: July 2018

SummaryHuman platelets were separated into density subpopulations by using a step-wise gradient of Percoll in Tris-NaCl buffer. The absolute value of the electrophoretic mobility (EPM) of the density subpopulations was found to be a linear function of the density of the platelets, with EPM becoming less negative with increasing platelet density. Platelet volume distributions, mode volume, and sialic acid and protein contents were found to increase with platelet density, while no differences were found in GPU, GPIII, and GPIV contents among the subpopulations. An estimate of charge density was made from the ratio between the PAS-staining material (membrane GP’s) and platelet surface area. The ratio was found to decrease as platelet density increased, consistent with the less negative EPM values observed for the higher density platelets. This lower surface charge of heavier platelets, which would lower charge repulsion between cells, agrees with the premise that heavier platelets are more active.
Platelet Activation in Psoriasis
Authors: Berrettini Mauro , Parise Pasquale , Costantini Vincenzo , Grasselli Serena , Nenci Giuseppe G 
Published: July 2018

SummaryRecent epidemiological studies have suggested that psoriasis represents a risk factor for thrombotic vascular diseases. In order to evaluate the possible role of hemostatic changes in the development of thrombotic episodes in psoriasis, some parameters of the hemostatic “balance” were investigated in 22 male psoriatic patients and compared to those of 22 male control subjects. Incidence of known risk factors for vascular diseases (diabetes, hypertension, smoking, dyslipidemia) was comparable in the two study groups. There were no statistically significant differences in platelet count, circulating platelet aggregates, platelet production of malondialdehyde (MDA), total plasma anti thrombin and fibrinolytic activities. In patients with psoriasis the incidence of spontaneous platelet hyperaggregability and plasma levels of β-thromboglobulin were significantly higher than in control subjects. Platelet regeneration time, measured as MDA recovery after aspirin ingestion, was significantly shorter in psoriatic patients. These data suggest that an in vivo platelet activation occurs in patients with psoriasis and could contribute to the development of thrombotic complications. The release of mitogenic and inflammatory substances by activated platelets may play a role in the histogenesis of psoriatic lesions.
Determination of Plasma Protein S - The Protein Cofactor of Activated Protein C
Authors: Bertina R M , van Wijngaarden A , Reinalda-Poot J , Poort S R , Bom V J J 
Published: July 2018

SummaryProtein S, an important cofactor of activated protein C, and C4b-binding protein were purified from human plasma. Specific antibodies against the purified proteins were raised in rabbits and used for the development of immunologic assays for these proteins in plasma: an immunoradiometric assay for protein S (which measures both free protein S and protein S complexed with C4b-binding protein) and an electroimmunoassay for C4b- binding protein. Ranges for the concentrations of these proteins were established in healthy volunteers and patients using oral anticoagulant therapy. A slight decrease in protein S antigen was observed in patients with liver disease (0.78 ± 0.25 U/ml); no significant decrease in protein S was observed in patients with DIC (0.95 ± 0.25 U/ml).Criteria were developed for the laboratory diagnosis of an isolated protein S deficiency
An Inhibitor to Factor VIII: C in a Patient with Possible Combined Haemophilia A and von Willebrand’s Disease
Authors: Taylor L D , Dean F L , Tiedemann K , Ekert H 
Published: July 2018

SummaryA factor VIII inhibitor has been found in a patient with an unusual combination of factor VIII-related properties. The inhibitor is directed specifically against the clotting activity (VIII :C) of the factor VIII complex. It behaves in a similar fashion to high responding inhibitors of factor VIII seen in haemophilia A patients and it was characterised as an immunoglobulin of the IgG class. Laboratory results from the patient and his family show considerable variation of factor VUI-related properties between various individuals. Overall, the data suggests the co-existence of haemophilia A and von Willebrand’s disease in the family and the presence of both diseases in the patient.