bluuebunny/crossref_metadata_2025_split huggingface dataset:
Folliculin Regulates Osteoclastogenesis Through Metabolic Regulation
Authors: Baba Masaya , Endoh Mitsuhiro , Ma Wenjuan , Toyama Hirofumi , Hirayama Akiyoshi , Nishikawa Keizo , Takubo Keiyo , Hano Hiroyuki , Hasumi Hisashi , Umemoto Terumasa , Hashimoto Michihiro , Irie Nobuko , Esumi Chiharu , Kataoka Miho , Nakagata Naomi , Soga Tomoyoshi , Yao Masahiro , Kamba Tomomi , Minami Takashi , Ishii Masaru , Suda Toshio 
Published: June 2018

ABSTRACT

Osteoclast differentiation is a dynamic differentiation process, which is accompanied by dramatic changes in metabolic status as well as in gene expression. Recent findings have revealed an essential connection between metabolic reprogramming and dynamic gene expression changes during osteoclast differentiation. However, the upstream regulatory mechanisms that drive these metabolic changes in osteoclastogenesis remain to be elucidated. Here, we demonstrate that induced deletion of a tumor suppressor gene, Folliculin (Flcn), in mouse osteoclast precursors causes severe osteoporosis in 3 weeks through excess osteoclastogenesis. Flcn-deficient osteoclast precursors reveal cell autonomous accelerated osteoclastogenesis with increased sensitivity to receptor activator of NF-κB ligand (RANKL). We demonstrate that Flcn regulates oxidative phosphorylation and purine metabolism through suppression of nuclear localization of the transcription factor Tfe3, thereby inhibiting expression of its target gene Pgc1. Metabolome studies revealed that Flcn-deficient osteoclast precursors exhibit significant augmentation of oxidative phosphorylation and nucleotide production, resulting in an enhanced purinergic signaling loop that is composed of controlled ATP release and autocrine/paracrine purinergic receptor stimulation. Inhibition of this purinergic signaling loop efficiently blocks accelerated osteoclastogenesis in Flcn-deficient osteoclast precursors. Here, we demonstrate an essential and novel role of the Flcn-Tfe3-Pgc1 axis in osteoclastogenesis through the metabolic reprogramming of oxidative phosphorylation and purine metabolism. © 2018 The Authors Journal of Bone and Mineral Research published by Wiley Periodicals, Inc. on behalf of American Society for Bone and Mineral Research (ASBMR).
Annexin A7 is correlated with better clinical outcomes of patients with breast cancer
Authors: Huang Yuanli , Wang Hongtao , Yang Yuanrong 
Published: June 2018

AbstractThe clinical prognosis of breast cancer (BC) patients remains poor. Studies on BC microarrays GSE37751, GSE7390, and GSE21653 were reanalyzed to characterize the expressions of annexin A7 (ANXA7) in BC patients and the corresponding normal breast tissues and the correlation between ANXA7 expression and clinical characteristics and survivals of BC patients. Gene set enrichment analysis (GSEA) was applied to investigate the exact mechanisms as for the expression of ANXA7 and the proliferation of BC cells. The level of ANXA7 expression was significantly decreased in BC patients than that in normal controls (P < .0001). BC patients in the ANXA7 high‐expression group were associated with better clinical features such as tumor size; histopathological grading; estrogen receptors; and clinical risk groups according to St Gallen criteria, Nottingham prognostic index criteria, and Veridex signature compared with those in the ANXA7 low‐expression group. Higher expression of ANXA7 predicted better prognosis of BC patients. The result of GSEA indicated that ANXA7 might inhibit the proliferation of BC cells through biological processes involved in androgen response, heme metabolism, and oxidative phosphorylation. The messenger RNA and protein levels of ANXA7 were decreased in BC tissues compared with those in normal breast tissues. Our results proved that ANXA7 was downregulated in BC cells and that a higher expression of ANXA7 was associated with better prognosis of BC patients.
Supporting adults to support youth: An evaluation of two social justice trainings
Authors: Anderson Amy J. , Sánchez Bernadette , Meyer Graig , Sales Brian P. 
Published: June 2018

AbstractThe current research examined pre‐ and post survey data in two social justice trainings for adults who support youth, including staff and volunteers (Study 1, N = 44) at youth‐serving organizations and adults who mentor young Black men and boys (Study 2, N = 72). We investigate whether training participants’ self‐report scores of cultural competences (studies 1 and 2), self‐efficacy for race equity (Study 1) and racial self‐efficacy to support mentee (Study 2) changed between the beginning of the training and after the training. Furthermore, we examine whether the changes depend upon whether training participants share cultural background with the youth with whom they work. In Study 1, findings indicated that training participants reported significantly increased scores of cultural sensitivity and self‐efficacy for race equity. Individuals who were not first‐generation college students saw higher increases in sociopolitical awareness. In Study 2, participants reported significantly higher scores of cultural sensitivity and racial self‐efficacy to support their mentees. Furthermore, non‐Black and women participants showed greater increases in self‐efficacy than Black and male participants. These results fill a gap in the literature on the potential role of social justice trainings to increase cultural competencies among adults who work with diverse youth.
An extraction‐free method for quantifying cell‐free DNA in the serum of cancer patients
Authors: Xie Li , Guo Wei , Liu Lisheng , Wei Ling , Song Xingguo , Song Xianrang 
Published: June 2018

AbstractBACKGROUNDCell‐free DNA (cfDNA) is an important marker for cancer diagnosis and monitoring. Extraction of cfDNA from serum is indispensable for its quantification, and the DNA isolation procedure can significantly influence the quantification and qualification of DNA.RESULTSA cfDNA‐extraction‐free (EF) method and a SuperGreen quantitative PCR assay for determining total cfDNA in serum were developed. The cfDNA from the EF method did not affect the specificity and sensitivity of quantitative polymerase chain reaction (qPCR) assay. It was found that the median amount of spike‐in cfDNA quantified by different isolation methods varied from 3.01 to 83.81 ng mL−1. The efficiency of DNA extraction by the EF method was significantly better than that in the case of other cfDNA extraction kits. The serum samples from the patients with nonsmall cell lung cancer (NSCLC) contained a higher concentration of cfDNA than that in healthy people, and cfDNA showed a better prognostic ability for diagnosing NSCLC.CONCLUSIONThe cfDNA‐EF method gives a more reliable, easy, and low‐cost estimate of total cell‐free DNA quantity in serum. © 2018 Society of Chemical Industry
Quality of life outcomes in transcatheter aortic valve replacement patients requiring pacemaker implantation
Authors: Bhardwaj Aishwarya , Ramanan Tharmathai , Sawant Abhishek C. , Sinibaldi Everett , Pham Michael , Khan Sahoor , Qureshi Reema , Agrawal Nikhil , Khalil Charl , Hansen Rosemary , Baldo Shannon , Colern Gerald , Corbelli John , Pershad Ashish , Beck Hiroko , Iyer Vijay 
Published: June 2018

AbstractBackgroundPermanent pacemaker implantation is the most common complication after Transcatheter aortic valve replacement (TAVR) and is associated with worse outcomes and mortality. However, its impact on quality‐of‐life (QoL) outcomes remains unknown.MethodsWe included 383 consecutive patients undergoing TAVR from January 2012 to 2016 who completed a baseline Kansas City Cardiomyopathy Questionnaire (KCCQ‐12) health survey. The clinical, laboratory, angiographic, QoL, mortality, and occurrence of poor outcomes (KCCQ‐12 score < 45 or KCCQ decrease of ≥10 points) were obtained.ResultsThe mean age was 83 ± 8 years, 51% were men, and majority were Caucasians (n = 364, 95%). Permanent pacemaker (PPM) was implanted in 11.5% of patients post‐TAVR. PPM patients were more likely to have prior conduction disease including RBBB (25% vs 12%, P = .02) and PQ interval >250 ms (11% vs 5%, P = .07). One‐month median KCCQ‐12 scores were significantly lower among PPM patients (84.7 vs 68.8, P = .04), but did not differ significantly at 1‐year (86.5 vs 90.6, P = .5) post‐TAVR. Occurrence of poor outcomes did not differ significantly among those with or without PPM at 1 month (11% vs 7%, P = .39) and 1 year (13% vs 9%, P = .45), respectively. However, patients with poor QoL outcomes at 1 month post‐TAVR also had significantly worse mortality during follow‐up in unadjusted (31.3% vs 4.5%, P < .001) and adjusted (HR = 5.30, 95% [CI: 1.85‐15.22, P = .002])analyses, respectively.ConclusionPermanent pacemaker implantation is associated with short‐term reduction in QoL without long‐term implications post‐TAVR. Patients with poor QoL post‐TAVR also have significantly higher mortality.
Acute pulmonary hemorrhage during atrial fibrillation HotBalloon ablation
Authors: Fujii Eitaro , Fujita Satoshi , Kagawa Yoshihiko , Ito Masaaki 
Published: June 2018

AbstractA 64‐year‐old man with an atrial septal defect was referred for HotBalloon ablation of symptomatic drug‐resistant paroxysmal atrial fibrillation. Pulmonary vein (PV) isolation was achieved using a SATAKE HotBalloon ablation system, which was inserted into the left atrium through the deflectable guiding sheath via the atrial septal defect. During ablation of the right superior pulmonary vein carina, the HotBalloon dropped to the left atrium. Hemoptysis and respiratory failure was then observed, and the patient was intubated and controlled under ventilator. The computed tomography identified a pseudoaneurysm developed on the right superior PV, with massive hemorrhagic alveolar flooding.
Removal of hyperpolarized 129Xe gas‐phase contamination in spectroscopic imaging of the lungs
Authors: Hahn Andrew D. , Kammerman Jeff , Fain Sean B. 
Published: June 2018

PurposeA novel technique is presented for retrospective estimation and removal of gas‐phase hyperpolarized Xenon‐129 (HP 129Xe) from images of HP 129Xe dissolved in the barrier (comprised of parenchymal lung tissue and blood plasma) and red blood cell (RBC) phases. The primary aim is mitigating RF pulse performance limitations on measures of gas exchange (e.g., barrier–gas and RBC–gas ratios). Correction for gas contamination would simplify technical dissemination of HP 129Xe applications across sites with varying hardware performance, scanner vendors, and models.MethodsDigital lung phantom and human subject experiments (N = 8 healthy; N = 1 with idiopathic pulmonary fibrosis) were acquired with 3D radial trajectory and 1‐point Dixon spectroscopic imaging to assess the correction method for mitigating barrier and RBC imaging artifacts. Dependence of performance on TE, image SNR, and gas contamination level were characterized. Inter‐ and intra‐subject variation in the dissolved‐phase ratios were quantified and compared to human subject experiments before and after correction.ResultsGas contamination resulted in image artifacts similar to those in disease that were mitigated after correction in both simulated and human subject data; for simulation experiments performance varied with TE, but was independent of image SNR and the amount of gas contamination. Artifacts and variation of barrier and RBC components were reduced after correction in both simulation and healthy human lungs (barrier, P = 0.01; RBC, P = 0.045).ConclusionThe proposed technique significantly reduced regional variations in barrier and RBC ratios, separated using a 1‐point Dixon approach, with improved accuracy of dissolved‐phase HP 129Xe images confirmed in simulation experiments.
On the relation between MR spectroscopy features and the distance to MRI‐visible solid tumor in GBM patients
Authors: Pedrosa de Barros Nuno , Meier Raphael , Pletscher Martin , Stettler Samuel , Knecht Urspeter , Herrmann Evelyn , Schucht Philippe , Reyes Mauricio , Gralla Jan , Wiest Roland , Slotboom Johannes 
Published: June 2018

PurposeTo improve the detection of peritumoral changes in GBM patients by exploring the relation between MRSI information and the distance to the solid tumor volume (STV) defined using structural MRI (sMRI).MethodsTwenty‐three MRSI studies (PRESS, TE 135 ms) acquired from different patients with untreated GBM were used in this study. For each MRSI examination, the STV was identified by segmenting the corresponding sMRI images using BraTumIA, an automatic segmentation method. The relation between different metabolite ratios and the distance to STV was analyzed. A regression forest was trained to predict the distance from each voxel to STV based on 14 metabolite ratios. Then, the trained model was used to determine the expected distance to tumor (EDT) for each voxel of the MRSI test data. EDT maps were compared against sMRI segmentation.ResultsThe features showing abnormal values at the longest distances to the tumor were: %NAA, Glx/NAA, Cho/NAA, and Cho/Cr. These four features were also the most important for the prediction of the distances to STV. Each EDT value was associated with a specific metabolic pattern, ranging from normal brain tissue to actively proliferating tumor and necrosis. Low EDT values were highly associated with malignant features such as elevated Cho/NAA and Cho/Cr.ConclusionThe proposed method enables the automatic detection of metabolic patterns associated with different distances to the STV border and may assist tumor delineation of infiltrative brain tumors such as GBM.
A balanced load mapping method based on radial basis functions and fuzzy sets
Authors: Biancolini M.E. , Chiappa A. , Giorgetti F. , Groth C. , Cella U. , Salvini P. 
Published: June 2018

SummaryA common issue in multiphysics analysis regards a reliable way for loose couplings, because the same object is modeled using different mesh refinements, each one suited for a proper field of physics. Output data originating from a simulation environment are transferred as input data to a different model to run a new analysis. It is strongly desirable that such information transfers in a conservative way in terms of general balance. This paper faces the problem of pressure mapping between widely dissimilar meshes. The proposed procedure yields two steps: pressure interpolation by means of radial basis functions and fuzzy subset correction. The first step is pointwise interpolation that exploits a series of basis functions. The second step applies to the outcome of the first one to reestablish load balance between the two models through the introduction of a smooth correction field. Practical tests from the aeronautical field allow validating the method.
Sulfonated Compounds Bind with Prostatic Acid Phosphatase (PAP248–286) to Inhibit the Formation of Amyloid Fibrils
Authors: Zhang Tingting , Yang Haikui , Yang Zichao , Tan Suiyi , Jin Jiabin , Liu Shuwen , Zhang Jiajie 
Published: June 2018

AbstractThe peptide segment of prostatic acid phosphatase (PAP248–286) aggregates to form SEVI (semen‐derived enhancer of virus infection) amyloid fibrils. These are characteristic seminal amyloids that have the ability to promote the effect of HIV infection. In this paper, we explore the binding of sulfonated compounds with PAP248–286 through an in silico study. Three derivatives of suramin, NF110, NF279, and NF340, are selected. All of these sulfonated molecules bind to PAP248–286 and alter the conformation of the peptide, even though they have various structures, sizes, and configurations. The compounds bind with PAP248–286 through multiple interactions, such as hydrogen‐bonding interactions, hydrophobic interactions, π–π stacking interactions, and electrostatic interactions. However, NF110, which has an X‐shaped configuration, has the highest binding affinity of the three derivatives investigated. We also perform surface plasmon resonance and a Congo red assay to validate the results. The interactions between PAP248–286 and the sulfonated compounds are proposed to depend on the orientations of the sulfonate groups and the specific configurations of the compounds instead of the number of sulfonate groups. NF110 molecules occupy the exposed binding sites of PAP248–286, blocking interactions between the peptides. Therefore, these compounds are important in inhibiting the aggregation of PAP248–286. Herein, we provide useful information to develop new efficient microbicides to antagonize seminal amyloid fibrils and to block HIV transmission.
Are Reemployment Services Effective? Experimental Evidence from the Great Recession
Authors: Michaelides Marios , Mueser Peter 
Published: June 2018

AbstractWe examine an experimental‐design reemployment program implemented in Nevada during the Great Recession that required Unemployment Insurance (UI) recipients to: (1) undergo an eligibility review to confirm they were qualified for benefits and actively searching for work and, if deemed eligible, (2) receive job‐counseling services. Our results show that the program expedited participant exit from UI, produced UI savings that exceeded program costs, and improved participant employment outcomes. Analyses of program effects on the UI exit likelihood show that the program's effects are partly associated with increased participant exit up through the time when program activities were scheduled, reflecting voluntary exit of participants from UI to avoid program activities and disqualifications of participants who failed to meet eligibility requirements. In addition, the program induced substantial participant exit from UI in the period after participants fulfilled requirements and their interactions with the program had ended, suggesting that the job‐counseling services offered by the program may have helped participants to conduct more effective job searches. Our findings provide evidence that reemployment programs that combine an eligibility review with mandatory participation in job‐search services can be effective during recessions.
Using the MEDiPORT humanoid robot to reduce procedural pain and distress in children with cancer: A pilot randomized controlled trial
Authors: Jibb Lindsay A. , Birnie Kathryn A. , Nathan Paul C. , Beran Tanya N. , Hum Vanessa , Victor J. Charles , Stinson Jennifer N. 
Published: June 2018

AbstractBackgroundSubcutaneous port needle insertions are painful and distressing for children with cancer. The interactive MEDiPORT robot has been programmed to implement psychological strategies to decrease pain and distress during this procedure. This study assessed the feasibility of a future MEDiPORT trial. The secondary aim was to determine the preliminary effectiveness of MEDiPORT in reducing child pain and distress during subcutaneous port accesses.MethodsThis 5‐month pilot randomized controlled trial used a web‐based service to randomize 4‐ to 9‐year‐olds with cancer to the MEDiPORT cognitive‐behavioral arm (robot using evidence‐based cognitive‐behavioral interventions) or active distraction arm (robot dancing and singing) while a nurse conducted a needle insertion. We assessed accrual and retention; technical difficulties; outcome measure completion by children, parents, and nurses; time taken to complete the study and clinical procedure; and child‐, parent‐, and nurse‐rated acceptability. Descriptive analyses, with exploratory inferential testing of child pain and distress data, were used to address study aims.ResultsForty children were randomized across study arms. Most (85%) eligible children participated and no children withdrew. Technical difficulties were more common in the cognitive‐behavioral arm. Completion times for the study and needle insertion were acceptable and >96% of outcome measure items were completed. Overall, MEDiPORT and the study were acceptable to participants. There was no difference in pain between arms, but distress during the procedure was less pronounced in the active distraction arm.ConclusionThe MEDiPORT study appears feasible to implement as an adequately‐powered effectiveness‐assessing trial following modifications to the intervention and study protocol.ClinicalTrials.gov NCT02611739.
A case of inherited type 1 and type 2A von Willebrand disease confirmed by diagnostic exome sequencing
Authors: Shim Ye Jee , Park So Yun , Jung Nani , Kim Heung Sik , Ha Jung‐Sook , Jang Ja‐Hyun 
Published: June 2018

AbstractA 10‐year‐old male and his family members visited a pediatric hematology clinic due to coagulopathy. Laboratory tests indicated von Willebrand disease (vWD) in all the family members. We conducted diagnostic exome sequencing for confirmation. The patient was confirmed to be a compound heterozygote for vWD: c.2574C > G (p.Cys858Trp) from his father (known variant of vWD type 1) and c.3390C > T (p.Pro1127_Gly1180delinsArg) from his mother (variant known to result in exon 26 skipping in vWD type 2A). He was managed with factor VIII and von Willebrand factor complex concentrate during palatoplasty due to bleeding despite pre‐operative desmopressin injection. The operation was completed successfully.
Unfavorable presentation but comparable outcome: Presentation and outcome of children with nodular lymphocyte predominant Hodgkin lymphoma from India
Authors: Prasad Maya , Narula Gaurav , Chinnaswamy Girish , Arora Brijesh , Shet Tanuja , Panjwani Poonam , Sengar Manju , Laskar Siddharth , Khanna Nehal , Banavali Shripad 
Published: June 2018

AbstractBackgroundNodular lymphocyte predominant Hodgkin lymphoma (NLPHL) is an uncommon subtype of Hodgkin lymphoma (HL) with few published studies in children, entirely from North America and Europe. We analyzed clinical features and treatment outcome of pediatric NLPHL.ProcedureChildren less than 18 years of age diagnosed after histopathology review to have NLPHL between June 1998 and August 2016 were retrospectively analyzed. Descriptive details of clinical presentation and treatment were collected, and outcomes analyzed using Kaplan–Meier survival analysis.ResultsOf the 42 patients with a confirmed diagnosis of NLPHL during this period, there was complete information on 35. Median age was 11 years (range 6–16 years), male:female ratio was 4.8:1, there were 15, 11, 6, and 3 patients with Stage I, Stage II, Stage III, and Stage IV disease, respectively. Six patients had B symptoms, 10 had bulky disease, and 3 had bone marrow as well as extranodal involvement. Histology was typical NLPHL in 23 and variant in 12. Twenty‐nine received chemotherapy, 10 with additional radiation, 3 patients with early stage disease received only radiotherapy and three others underwent complete node resection alone. Median follow‐up was 55 months (range 7–165 months), 5 year event‐free survival (EFS) was 83.3%, and overall survival 97.1%. Variant NLPHL histology was associated with higher incidence of unfavorable presentation and lower EFS.ConclusionsNLPHL in India has an excellent outcome, despite a higher incidence of unfavorable presentations such as advanced stage disease, B symptoms, and bulky disease. Variant histology is an adverse prognostic factor.