bluuebunny/crossref_metadata_2025_split huggingface dataset:
High-throughput proliferation and activation of NK-92MI cell spheroids via a homemade one-step closed bioreactor in pseudostatic cultures for immunocellular therapy
Authors: Lin Jhih-Ni , Kuan Che-Yung , Chang Chia-Ting , Chen Zhi-Yu , Kuo Wei-Ting , Lin Jason , Lin Yu-Ying , Yang I.-Hsuan , Lin Feng-Huei 
Published: November 2024

AbstractThe NK-92MI cell line has displayed significant promise in clinical trials for cancer treatment. However, challenges persist in obtaining sufficient cell quantities and achieving optimal cytotoxicity. The proliferation of natural killer (NK) cells involves the formation of cell aggregates, but excessively large aggregates can impede nutrient and waste transport, leading to reduced cell survival rates. In this study, a custom bioreactor was designed to mimic pseudostatic culture conditions by integrating brief mechanical rotation during a 6-h static culture period. This method aimed to achieve an optimal aggregate size while improving cell viability. The findings revealed a 144-fold expansion of 3D NK-92MI cell aggregates, reaching an ideal size of 80–150 µm, significantly increasing both cell proliferation and survival rates. After 14 days of culture, the NK-92MI cells maintained their phenotype during the subsequent phase of cell activation. Moreover, these cells presented elevated levels of IFN-γ expression after IL-18 activation, resulting in enhanced NK cell-mediated cytotoxicity against K562 cells. This innovative strategy, which uses a closed suspension-based culture system, presents a promising approach for improving cell expansion and activation techniques in immunocellular therapy.
Postpartum modern family planning among women living with HIV attending care at health facilities in Busia County, Kenya
Authors: Tebagalika Florence Zawedde , Kimuli Derrick , Walusimbi Dennis , Nyang’echi Edna , Ndunyu Louisa 
Published: November 2024

Abstract
Background
For women living with the human immunodeficiency virus (WLHIV), preventing untimed pregnancies during the postpartum period reduces vertical transmission and improves other maternal and child health outcomes. In Kenya, Busia County’s HIV prevalence and mother-to-child transmission rate are higher than the national average yet uptake of postpartum family planning (PPFP) is generally low. This study examined health system factors influencing the consistent use of PP modern FP methods among WLHIV in Busia County.

Methods
A retrospective study involving 314 WLHIV with children aged 12–24 months who were chosen using systematic random sampling was conducted from February to March 2024 from outpatient clinics in Busia County. Additionally, 14 health providers were purposively sampled as key informants. Quantitative data was collected using a pretested questionnaire, while qualitative data was gathered through key informant interview guides. Quantitative data was analyzed using STATA 15 with descriptive statistics, logistic regression, and Chi-square tests, while a deductive thematic analysis was used for qualitative data.

Results
The mean age of the participants was 32.06 (± 6.00) with the majority (51.27) aged between 25 and 34 years, married (74.84%) and unemployed (77.39%). Overall, 73.25% had used postpartum (PP) modern family planning (FP) methods, but only 52.55% reported consistent use throughout the first year postpartum. The only factors found to increase the odds of PPFP use were being married (aOR 3.34, 95% CI 1.58–7.07, p = 0.002), being escorted by a preferred person during seeking maternal and child health services (aOR 2.29, 95% CI 1.36–3.83, p = 0.002), and perceiving that they were provided information on all types of FP (aOR 2.33, 95% CI 1.19–4.16, p = 0.012). Persistent stock-outs and inadequate counseling hindered consistent PPFP use.

Conclusion
The study identified gaps in the consistent use of PP modern FP methods among WLHIV in Busia County, influenced by the availability of FP information and health system factors. Addressing stock-outs and improving counseling during clinic visits and pregnancy are crucial for improving FP service delivery and reducing maternal and child health risks in high HIV-incidence areas like Busia County.
Is retinal vein occlusion highly associated with an increased risk of myocardial infarction? A systematic review and meta-analysis
Authors: Chen Kai-Yang , Chan Hoi-Chun , Chan Chi-Ming 
Published: November 2024

Abstract
Background and objective
Retinal vein occlusion (RVO) and acute myocardial infarction (MI) are significant vascular events that impact patient health and mortality. Both conditions share common risk factors, such as hypertension, diabetes, and atherosclerosis. This study investigated the potential connection between RVO and MI, particularly among younger individuals, to improve preventive measures and management protocols.

Method
A systematic review and meta-analysis were conducted, adhering to the PRISMA and MOOSE guidelines. Multiple databases, including PubMed, Scopus, MEDLINE, ScienceDirect, and ClinicalTrials.gov, were exhaustively searched until August 24, 2024. Studies were selected based on their reports of the association between RVO and MI risk. Quality assessment was performed using the Newcastle-Ottawa Quality Assessment Scale, and data were pooled using a random-effects model with hazard ratios and 95% confidence intervals.

Result
Twelve studies comprising 371,817 participants were included. Meta-analysis revealed a pooled hazard ratio of 1.324 (95% CI, 1.238–1.415), indicating a significant association between RVO and increased MI risk (p = 0.0001). Subgroup analysis for central retinal vein occlusion (CRVO) showed a hazard ratio of 1.691 (95% confidence interval [CI] 1.142, 2.502, p = 0.009) with moderate heterogeneity (I2 = 36%), whereas branch retinal vein occlusion (BRVO) yielded a non-significant hazard ratio of 1.167 (95% CI 0.843, 2.106, p = 0.444; I2 = 33%). Publication bias was identified (Egger’s test, p = 0.036) and addressed through trim-and-fill adjustment, maintaining statistical significance.

Conclusion
Our meta-analysis shows a strong association between CRVO and a 69.1% increased risk of MI, while BRVO shows no significant correlation. Overall, RVO is linked to a 32.4% elevated risk of MI. Despite slight publication bias, adjusted analyses confirm reliability, indicating that improved cardiovascular monitoring for RVO patients, especially those with CRVO, is essential to mitigate MI risk.

Clinical trial number
Not applicable.
Specialty Tobacco Treatment Implementation in Oncology: A Qualitative Study
Authors: Bird Jennifer E. , Chladek Jason S. , D'Angelo Heather , Minion Mara , Pauk Danielle , Adsit Robert T. , Conner Karen L. , Zehner Mark , Fiore Michael , Rolland Betsy , McCarthy Danielle 
Published: November 2024

PURPOSE
In 2017, the National Cancer Institute (NCI) funded the Cancer Center Cessation Initiative (C3I) to implement and expand tobacco treatment programs in routine oncology care. Many C3I programs developed specialty care programs staffed by tobacco treatment specialists (TTSs) to deliver evidence-based treatment to adult patients who smoke. People involved in specialty tobacco treatment programs can help to identify implementation strategies and adaptations that may enhance tobacco treatment reach and effectiveness in cancer care and help more patients with cancer quit using tobacco.

METHODS
We conducted semistructured interviews with TTSs from 21 C3I-funded cancer centers and applied content analysis to interview transcripts from 37 TTSs and 17 respondents in other program roles. We used the Consolidated Framework for Implementation Research to code interview data. We identified final themes and implementation strategies and adaptations recommended by respondents on the basis of these codes.

RESULTS
Respondents shared that implementation of specialty tobacco treatment programs in cancer care settings could be facilitated by training staff to provide patient connection to services, incorporating prescription of no- or low-cost cessation medications, hiring additional staff to deliver tobacco treatment, allocating space to the program, and automating electronic health record workflows.

CONCLUSION
TTSs and others involved in specialty tobacco treatment in NCI-designated cancer centers identified ways to improve tobacco treatment access, use, and effectiveness by (1) adapting specialty tobacco treatment delivery to meet patient needs; (2) facilitating referrals and pharmacotherapy coordination; and (3) committing staffing, space, and support resources to tobacco treatment programs. Key program participants suggested that these approaches would help more oncology patients connect with evidence-based tobacco treatment and quit smoking.
Cigarette Smoking Impairs Cardiorespiratory and Metabolic Response at Peak Incremental Exercise and during Recovery in Young, Physically Active Adults
Authors: Borrelli Marta , Doria Christian , Toninelli Nicholas , Longo Stefano , Coratella Giuseppe , Cè Emiliano , Rampichini Susanna , Esposito Fabio 
Published: November 2024

ABSTRACT

Purpose: Cigarette smoking (CS) induces systemic changes that impair cardiorespiratory and muscular function both at rest and during exercise. Although these abnormalities are reported in sedentary, middle-aged smokers (SM) with pulmonary disease, few and controversial studies focused on young, physically active SM at the early stage of smoking history. This study aimed at assessing the impact CS on cardiorespiratory and metabolic response during an incremental test and the subsequent recovery in young, physically active SM without known lung or cardiovascular disease.

Methods: After pulmonary function evaluation, 12 SM (age: 22±2 yr; body mass: 75±8 kg; stature: 1.78±0.06 m; 12±4 cigarette/day for 6±2 years; mean±SD) and 12 non-smokers (CTRL; age: 23±1 yr; body mass: 76±8 kg; stature: 1.79±0.08 m) matched for age and exercise habits, underwent an exhaustive incremental step test (25 W/2 min) on a cycle ergometer. Pulmonary O2 uptake (V’O2), expiratory ventilation (V’E), heart rate (f
H) responses and lactate concentration were assessed during the test and subsequent recovery.

Results: Despite similar static lung volumes, SM reported lower peak expiratory flow (-23%; P = 0.003) and maximal voluntary ventilation (-10%; P = 0.003). At submaximal exercise, no differences in the cardiorespiratory and metabolic were noted between the two groups. However, SM exhibited ventilatory (P < 0.01) and lactate thresholds at lower work rates (P = 0.01). At peak exercise, SM exhibited lower V’O2 (-8%; P = 0.02), mechanical power (-11%; P = 0.02) and V’E (-9%; P = 0.01). During recovery, SM showed longer time constants (τ) in V’O2 (+52%; P = 0.002), V’E (+19%; P = 0.027) and f
H (+21%; P = 0.022) and smaller f
H at 30 s of recovery (HRR30; -31%; P = 0.032).

Conclusions: These results are compatible with an early CS-related impairment of the cardiorespiratory and metabolic function even in young individuals with relatively short smoking history.
Predictors of Resistance Training Behavior among Glucagon-like Peptide 1 Medication Users
Authors: Kompf Justin M. , Rhodes Ryan E. 
Published: November 2024

ABSTRACT

Introduction
Obesity is a chronic condition affecting around 650 million adults globally, with significant health implications such as cardiovascular disease and type 2 diabetes. Glucagon-like peptide 1 (GLP-1) medications have shown efficacy in promoting weight loss among obese individuals, although the weight loss is associated with undesired muscle breakdown. In this study, we investigate the psychosocial determinants of resistance training (RT) behavior among GLP-1 medication users, utilizing the Capability, Opportunity, and Motivation Behavior (COM-B) model.

Methods
A 1-wk longitudinal study design was adopted, involving members of a medical weight loss program (95.3% female; body mass index, 32.8 ± 7.0 kg·m−2, 48.8 ± 9.9 yr old), assessing correlates of RT participation through an online survey.

Results
Psychological capabilities (b = 0.39, standard error (SE) = 0.14; P = 0.004) and reflective motivation had direct effects on frequency (b = 1.27, SE = 0.52; P = 0.02). Automatic motivation (b = 3.40, SE = 1.22; P = 0.005) and physical opportunities (b = 2.05, SE = 0.92; P = 0.02) had direct effects on duration, and psychological capabilities (b = 0.41, SE = 0.18; P = 0.03) and automatic motivation (b = 0.27, SE = 0.13; P = 0.04) had direct effects on intensity. Psychological capabilities directly or indirectly influenced all RT participation characteristics, suggesting a critical role of planning and self-monitoring in fostering RT adherence.

Conclusion
For GLP-1 users engaging in RT, targeted behavioral interventions may be useful to mitigate muscle loss. Behavior change strategies should focus on psychological capabilities integrating planning and self-monitoring to enhance RT participation, with future research needed to confirm these results in more diverse and larger populations.
Research on Ecological Restoration Strategies for Abandoned Mines Based on Ecology-Oriented Development
Authors: Wang Ning , Tan Deqing 
Published: November 2024

The ecological restoration of abandoned mines poses a significant challenge within China’s broader efforts toward the protection and restoration of the nation’s ecosystems. Based on the ecology-oriented development (EOD) model, a differential game framework for the ecological restoration of abandoned mines was constructed in this study, involving both government and corporate entities as players. Our analysis focused on various factors, including investment levels in ecological restoration quality, the quality of ecological restoration itself, investment levels in environmentally sensitive industry development, the quality of environmentally sensitive industries, environmentally sensitive product pricing strategies, and profit dynamics for both players across four distinct modes. Our findings reveal several key insights. First, extending the government’s concession period incentivizes enterprises to increase investment in both the quality of ecological restoration of abandoned mines and the development of environmentally sensitive industries. Second, the government should prefer the cooperative mode to introduce social capital for the ecology-oriented development of abandoned mines. Third, in noncooperative mode, for technology-intensive industries, the government’s industrial support policy is more mutually beneficial; conversely, for labor-intensive industries, the ecological restoration compensation policy yields higher mutual gains. Lastly, differentiated incentive policies implemented by the government across various modes prompt the input of enterprises in ecological restoration quality and environmentally sensitive industry development. Based on these findings, we offer specific policy recommendations to guide governmental efforts in promoting EOD for the ecological restoration of abandoned mines. Funding: This work was supported by the Sichuan Office of Philosophy and Social Science [Grant SC22C004] and the National Social Science Fund of China [Grant 22XKS015].
Znanja i stavovi o nespuštenim testisima – potreba za edukativnom intervencijom
Authors: Bašković Marko , Čolić Tonko , Mašić Mario , Zaninović Luca , Čizmić Ante , Bojanac Ana Katušić , Ježek Davor 
Published: November 2024

Cilj: S obzirom na loše pokazatelje dobi orhidopeksije za kongenitalnu skupinu nespuštenih testisa, cilj ovoga rada bio je istražitiznanja i stavove specijalizanata/mladih specijalista pedijatrije, obiteljske i školske medicine o navedenoj problematici jer upravo suoni, u većini slučajeva prvi kojima se roditelji obraćaju za pomoć i mišljenje.Metode: Znanje i stavovi specijalizanata i mladih specijalista ispitani su pomoću anonimnog online upitnika, temeljenog na najnovijim spoznajama i smjernicama. Prikupljanje podataka provedeno je putem besplatnog programa Google Forms®. Kako bismoutvrdili odstupaju li dobiveni odgovori od odgovora koje bismo očekivali pod određenom hipotezom, odgovori su uspoređivani korištenjem hi-kvadrat testa uz razinu statističke značajnosti od 5%.Rezultati: Upitnik je ispunilo 129 specijalizanata/mladih specijalista, od kojih većina smatra kako za vrijeme studiranja i specijalističkog usavršavanja nisu dovoljno čuli i naučili o nespuštenim testisima, kako svoje znanje o problematici nespuštenih testisa smatrajunezadovoljavajućim te kako se u dosadašnjoj karijeri nisu susreli sa smjernicama koje govore o dijagnostici i liječenju nespuštenihtestisa. Odgovori na većinu pitanja, koja su se temeljila na najnovijim smjernicama, bili su nezadovoljavajući. Gotovo 2/3 ispitanikasmatra kako se o dječacima s nespuštenim testisima u Republici Hrvatskoj ne vodi pravodobna briga i liječenje, a 1/3 ispitanikaključni problem vidi u primarnim pedijatrima, obiteljskim liječnicima te u timovima školske medicine.Zaključci: S obzirom na dobro poznate posljedice nepravodobnog liječenja nespuštenih testisa u dječaka, rezultatima analize ankete kojima ne možemo biti zadovoljni potrebno je ozbiljno pristupiti. Stoga potičemo buduće edukativne intervencije, utemeljene nadokazima, koje će za cilj imati bolju informiranost o problematici nespuštenih testisa i u konačnici vremenski pomak samih orhidopeksija k ranijoj dobi.
Anafi laksija ovisna o hrani, potaknuta naporom – prikaz bolesnice s osvrtom na praktični pristup problemu
Authors: Topalušić Iva , Jureković Irena Ivković , Marković Asja Stipić , Jakuš Bernarda 
Published: November 2024

Anafi laksija ovisna o hrani, potaknuta naporom (engl. food dependent exercise induced anaphylaxis, FDEIA) poseban je oblik preosjetljivosti na hranu posredovan IgE protutijelima, koji se javlja u bolesnika koji unutar nekoliko sati od konzumacije određene namirnice budu izloženi tjelesnoj aktivnosti. U djece je posebice rijedak, a najčešće je uzrokovan pšeničnim brašnom, iako ga mogu uzrokovati i druge žitarice, ribe, plodovi mora, orašasti plodovi, kikiriki, jaje, mlijeko i druga hrana. Dijagnoza se postavlja temeljemkarakterističnih simptoma koji se pojavljuju tijekom ili nekoliko sati nakon tjelesne aktivnosti, kojoj prethodi ingestija određene hrane, in-vitro i in-vivo pretraga te provokacijski pokusi. Sama tjelesna aktivnost ili konzumacija hrane u odsustvu tjelesne aktivnosti, neizaziva simptome. U ovom radu prikazujemo 17-godišnju bolesnicu koja je pokazala FDEIA-u nakon konzumacije proizvoda odpšeničnog brašna. Iako rijedak, ovaj je entitet potrebno poznavati kako bi se izbjegle životno ugrožavajuće alergijske reakcije, a temelj dijagnoze je detaljna anamneza.
Neuronska ceroidna lipofuscinoza – najčešća neurodegenrativna bolest u djetinjstvu
Authors: Kravljanac Ružica 
Published: November 2024

Cilj: Neuronska ceroidna lipofuscinoza pripada skupini lizosomskih poremećaja, obuhvaća četrnaest tipova bolesti i najčešća je neurodegenerativna bolest u djetinjstvu. Cilj je ove publikacije da se liječnici upoznaju s načinom i značenjem ranog otkrivanja oboljelih od NCL-a.Metode: Analiza literature i podataka iz povijesti bolesti pacijenata liječenih u Institutu tijekom posljednjih 20 godina.Rezultati: Glavna karakteristika NCL-a je progresivni tijek bolesti koji zahvaća motoričko i kognitivno propadanje, epileptičke napadaje, a zbog zahvaćanja retine dolazi do progresivnog slabljenja vida sve do sljepila. Postoji genotipsko-fenotipska korelacija uzrastai početka ispoljavanja bolesti, brzina progresije, težine i kontrole epilepsije, izraženost psihijatrijskih poremećaja itd. Također postojikorelacija između genske mutacije i načina na koji se raspoređuju depoziti lipofuscina u stanici, a koji se vide elektronskim mikroskopom. Liječenje djece oboljele od neuronske ceroidne lipofuscinoze veliki je izazov. Palijativno liječenje koje obuhvaća i epilepsiju odključnog je značenja. Za liječenje pojedinih oblika NCL-a postoji mogućnost primjenom enzima koji nedostaje, kao što je slučaj kodkasnog infantilnog oblika (CLN2), ili genskom terapijom koja je u fazi kliničkog ili eksperimentalnog ispitivanja.Zaključak: Poznavanje ključnih kliničkih karakteristika, ranih simptoma i algoritma u dijagnostici NCL-a od velikog je značenja uranom otkrivanju bolesti. Rano otkrivanje dobiva na značenju u „novoj eri“ u kojoj je sve više lijekova za liječenje neuroprogresivnih,dosad neizlječivih bolesti kao što je i NCL, a važno je i u prenatalnoj dijagnostici.
Neurološke manifestacije mukopolisaharidoza
Authors: Kero Mijana 
Published: November 2024

Mukopolisaharidoze su skupina rijetkih progresivnih bolesti nakupljanja koje se javljaju u oko 1:25000 osoba. Uzrokovane su nasljednim djelomičnim ili potpunim nedostatkom lizosomnih enzima koji sudjeluju u razgradnji glikozaminoglikana. Djelomično razgrađeni glikozaminoglikani nakupljaju se u većini stanica i izvanstaničnom tkivu te se pojačano izlučuju urinom. Klinička slika je složena i može varirati od teških poremećaja, koji završavaju smrću već u prvim mjesecima ili godinama života, do onih koji se očituju sblagim simptomima i imaju normalan životni vijek. Česti simptomi uključuju dysostosis multiplex, kraniofacijalnu dizmorfi ju, hepatosplenomegaliju, ukočenost i kontrakture zglobova, kardiomiopatiju, bolest zalistaka, česte respiratorne infekcije, a u nekim oblicima je zahvaćen i središnji živčani sustav. Enzimsko nadomjesno liječenje za MPS tip I., II., IV.A, VI. i VII. uz transplantaciju koštane sržikod MPS-a I. poboljšale su prognozu u mnogih bolesnika. No enzimsko nadomjesno liječenje ne sprječava razvoj neuroloških manifestacija. Teška i životnoograničavajuća priroda neurokognitivnih problema kod bolesnika s MPS-om traži ranu dijagnozu i liječenjekako bi se ograničila ireverzibilna oštećenja tkiva. Učinkoviti tretmani MPS poremećaja moraju imati za cilj spriječiti ili zaustaviti razvoj kako somatskih tako i neuroloških manifestacija. Precizni klinički opis neuroloških manifestacija kod pacijenata, kao i razumijevanje patološke osnove u njihovoj podlozi, može pomoći u ranoj identifi kaciji bolesnika, dizajniranju kliničkih studija i razvojuučinkovitih terapija.
Seksualno ponašanje dviju generacija studenata u Osijeku
Authors: Han Kristina Hibler , Majer Marjeta , Jureša Vesna 
Published: November 2024

Cilj rada je bio istražiti seksualno ponašanje studenata prve godine Sveučilišta u Osijeku, akademske godine 2005./2006. i 2015./2016.te utvrditi razlike među spolovima i generacijama.Metode: Ispitanici u istraživanju bili su studenti Sveučilišta u Osijeku, njih ukupno 1229-ero, od toga 599 (47,1% djevojaka) akademske godine 2005./2006., te 630 studenata (52,5% djevojaka) akademske godine 2015./2016. Iz medicinske dokumentacije ispitanikaprimijenjeni su podatci o dobi, spolu i seksualnom ponašanju. Podatci su analizirani metodama deskriptivne statistike.Rezultati: Od ukupnog broja ispitanika 2005./2006. spolni odnos je imalo njih 65,4%, a 2015./2016. 62,9%. Dob prvog spolnog odnosa 2005./2006. za većinu mladića (41,0%) bila je 17 godina, a za djevojke 18 godina (38,9%), a 2015./2016. 18 godina (33,3%) za obaspola. Obje akademske godine najveći udio ispitanika (70,4% i 75,5%) imao je u posljednjih godinu dana jednog partnera. Kondomje redovito upotrebljavalo 62,0% i 65,7% ispitanika. Oralnu hormonsku kontracepciju primjenjivalo je značajno više djevojaka2005./2006. u odnosu na 2015./2016. (30% vs 15%; χ2=13,2; P=0,003). Prekinutim snošajem redovito se služilo 10,7% i 13,9% ispitanika. Sigurne dane redovito je primjenjivalo 9,4% i 19,2% ispitanika, značajno više djevojaka 2015./2016. u odnosu na 2005./2006.(27,8% vs 13,3%; χ2=12,5, P=0,006).Zaključak: Rezultati pokazuju nedostatnu uporabu oralne hormonske kontracepcije i kondoma među osječkim studentima, te porast uporabe tradicionalnih metoda kontracepcije, osobito među djevojkama, što upućuje na potrebu kontinuirane edukacijemladih u segmentu reproduktivnog zdravlja.