bluuebunny/crossref_metadata_2025_split huggingface dataset:
Alginate/chitosan hydrogel containing olfactory ectomesenchymal stem cells for sciatic nerve tissue engineering
Authors:  Salehi Majid , Bagher Zohreh , Kamrava Seyed Kamran , Ehterami Arian , Alizadeh Rafieh , Farhadi Mohammad , Falah Masoumeh , Komeili Ali 
Published: January 2019

AbstractRegeneration and functional recovery after peripheral nerve damage still remain a significant clinical problem. In this study, alginate/chitosan (alg/chit) hydrogel was used for the transplantation of olfactory ectomesenchymal stem cells (OE‐MSCs) to promote peripheral nerve regeneration. The OE‐MSCs were isolated from olfactory mucosa biopsies and evaluated by different cell surface markers and differentiation capacity. After creating sciatic nerve injury in a rat model, OE‐MSCs were transplanted to the injured area with alg/chit hydrogel which was prepared and well‐characterized. The prepared hydrogel had the porosity of 91.3 ± 1.27%, the swelling ratio of 379% after 240 min, weight loss percentages of 80 ± 5.56% after 14 days, and good blood compatibility. The 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide assay, 4′,6‐diamidino‐2‐phenylindole, and LIVE/DEAD staining were done to assay the behavior of OE‐MSCs on alg/chit hydrogel and the results confirmed that the hydrogel can provide a suitable substrate for cell survival. For functional analysis, alg/chit hydrogel with and without OE‐ MSCs was injected into a 3‐mm sciatic nerve defect of Wistar rats. The results of the sciatic functional index, hot plate latency, electrophysiological assessment, weight‐loss percentage of wet gastrocnemius muscle, and histopathological examination using hematoxylin–eosin and Luxol fast blue staining showed that utilizing alg/chit hydrogel with OE‐MSCs to the sciatic nerve defect enhance regeneration compared to the control group and hydrogel without cells.
Aspirin inhibits cancer stem cells properties and growth of glioblastoma multiforme through Rb1 pathway modulation
Authors: Pozzoli Giacomo , Marei Hany E. , Althani Asma , Boninsegna Alma , Casalbore Patrizia , Marlier Lionel N. J. L. , Lanzilli Giulia , Zonfrillo Manuela , Petrucci Giovanna , Rocca Bianca , Navarra Pierluigi , Sgambato Alessandro , Cenciarelli Carlo 
Published: January 2019

AbstractSeveral clinical studies indicated that the daily use of aspirin or acetylsalicylic acid reduces the cancer risk via cyclooxygenases (Cox‐1 and Cox‐2) inhibition. In addition, aspirin‐induced Cox‐dependent and ‐independent antitumor effects have also been described. Here we report, for the first time, that aspirin treatment of human glioblastoma cancer (GBM) stem cells, a small population responsible for tumor progression and recurrence, is associated with reduced cell proliferation and motility. Aspirin did not interfere with cell viability but induced cell‐cycle arrest. Exogenous prostaglandin E2 significantly increased cell proliferation but did not abrogate the aspirin‐mediated growth inhibition, suggesting a Cox‐independent mechanism. These effects appear to be mediated by the increase of p21
waf1 and p27
Kip1, associated with a reduction of Cyclin D1 and Rb1 protein phosphorylation, and involve the downregulation of key molecules responsible for tumor development, that is, Notch1, Sox2, Stat3, and Survivin. Our results support a possible role of aspirin as adjunctive therapy in the clinical management of GBM patients.
A Phase 1 Randomized Study of Single Intravenous Infusions of the Novel Nitroxyl Donor BMS‐986231 in Healthy Volunteers
Authors: Cowart Douglas , Venuti Robert P. , Lynch Kim , Guptill Jeffrey T. , Noveck Robert J. , Foo Shi Yin 
Published: January 2019

AbstractNitroxyl (HNO) is a reactive nitrogen molecule that has potential therapeutic benefits for patients with acute heart failure. The results of the first‐in‐human study for BMS‐986231, a novel HNO donor, are reported. The aim of this sequential cohort study was to evaluate the safety, tolerability, and pharmacokinetic profile of BMS‐986231 after 24‐ and 48‐hour intravenous infusions in healthy volunteers. Eighty subjects were randomized and dosed. Seven cohorts (stratum A) received BMS‐986231 0.1, 0.33, 1, 3, 5, 10, and 15 μg/kg/min or placebo, infused over 24 hours. An additional cohort (stratum B) received 10 μg/kg/min or placebo, infused over 48 hours. Adverse events (AEs) were reported for 30 days after completion of infusion. Blood/urine samples were collected at regular intervals; other parameters (blood pressure, heart rate/rhythm, cardiac index) were also assessed. Headaches were the most commonly reported drug‐related AE (48%) in those who received BMS‐986231, although their severity was reduced by hydration. No other significant drug‐related AEs were noted. BMS‐986231 was associated with dose‐dependent and well‐tolerated reductions in systolic and diastolic blood pressure versus baseline; cardiac index, as measured noninvasively, was increased. BMS‐986231 had no clinically significant effect on heart rate/rhythm or laboratory parameters. Its mean elimination half‐life was 0.7‐2.5 hours. BMS‐986231 was safe and well‐tolerated for up to 24 hours (15 μg/kg/min) or 48 hours (10 μg/kg/min), with a favorable hemodynamic profile observed. Ongoing studies continue to evaluate the potential benefit of BMS‐986231 in patients with acute heart failure.
Fecograph: A graphical representation of daily stool forms to subtype irritable bowel syndrome in office practice
Authors: Shah Chirag , Grover Anumeet S , Dhore Prashant , Shukla Akash , Abraham Philip , Bhatia Shobna J 
Published: January 2019

Background and AimInterpreting stool form diaries for subtyping patients with the irritable bowel syndrome (IBS) is cumbersome; a picture showing a trend would be easier to interpret.MethodsFifty‐one consecutive adults with IBS (median age 35.5 years; 47 men), diagnosed using the Rome III criteria, were given a picture of the Bristol stool form scale (BSFS) and asked to record their stool frequency and form for 7 days. The numbers were plotted by a technician as dots on a chart. On the y axis, BSFS category 4 was marked as 0, harder stools as +1 to +3, and softer stools as −1 to −3; each bowel movement was represented on the x axis. A line graph was plotted by connecting the dots. Each “fecograph” was then given for visual interpretation to three gastroenterologists (observers). When most readings appeared to be 0, +1, or −1, it was to be reported as normal; most above +1 as IBS‐constipation (IBS‐C); most below −1 as IBS‐diarrhea (IBS‐D); and readings crossing 1 on either side as IBS‐mixed (IBS‐M). If no clear trend was noted, it was IBS‐unclassified (IBS‐U). Each observer reported all graphs in different orders twice, at 1‐month intervals; thus, 306 reports were available. Interclass correlation coefficient (ICC) was calculated.ResultsEighteen patients had IBS‐C, 13 IBS‐D, 4 IBS‐M, and 16 IBS‐U. The 51 fecographs were reported in mean 20 min 36 s. ICC for intra‐ and interobserver reliability was 0.62 (0.50–0.73).ConclusionThe fecograph is a reliable and easy‐to‐use tool to subtype patients with IBS.
On the clique number of the square of a line graph and its relation to maximum degree of the line graph
Authors: Faron Maxime , Postle Luke 
Published: January 2019

AbstractIn 1985, Erdős and Nešetřil conjectured that the square of the line graph of a graph , that is, , can be colored with colors. This conjecture implies the weaker conjecture that the clique number of such a graph, that is, , is at most . In 2015, Śleszyńska‐Nowak proved that . In this paper, we prove that . This theorem follows from our stronger result that where .
Pilot study on longitudinal change in pancreatic proton density fat fraction during a weight‐loss surgery program in adults with obesity
Authors: Covarrubias Yesenia , Fowler Kathryn J. , Mamidipalli Adrija , Hamilton Gavin , Wolfson Tanya , Leinhard Olof Dahlqvist , Jacobsen Garth , Horgan Santiago , Schwimmer Jeffrey B. , Reeder Scott B. , Sirlin Claude B. 
Published: January 2019

BackgroundQuantitative‐chemical‐shift‐encoded (CSE)‐MRI methods have been applied to the liver. The feasibility and potential utility CSE‐MRI in monitoring changes in pancreatic proton density fat fraction (PDFF) have not yet been demonstrated.PurposeTo use quantitative CSE‐MRI to estimate pancreatic fat changes during a weight‐loss program in adults with severe obesity and nonalcoholic fatty liver disease (NAFLD). To explore the relationship of reduction in pancreatic PDFF with reductions in anthropometric indices.Study TypeProspective/longitudinal.PopulationNine adults with severe obesity and NAFLD enrolled in a weight‐loss program.Field Strength/SequenceCSE‐MRI fat quantification techniques and multistation‐volumetric fat/water separation techniques were performed at 3 T.AssessmentPDFF values were recorded from parametric maps colocalized across timepoints.Statistical TestsRates of change of log‐transformed variables across time were determined (linear‐regression), and their significance assessed compared with no change (Wilcoxon test). Rates of change were correlated pairwise (Spearman's correlation).ResultsMean pancreatic PDFF decreased by 5.7% (range 0.7–17.7%) from 14.3 to 8.6%, hepatic PDFF by 11.4% (2.6–22.0%) from 14.8 to 3.4%, weight by 30.9 kg (17.3–64.2 kg) from 119.0 to 88.1 kg, body mass index by 11.0 kg/m2 (6.3–19.1 kg/m2) from 44.1 to 32.9 kg/m2, waist circumference (WC) by 25.2 cm (4.0–41.0 cm) from 133.1 to 107.9 cm, HC by 23.5 cm (4.5–47.0 cm) from 135.8 to 112.3 cm, visceral adipose tissue (VAT) by 2.9 L (1.7–5.7 L) from 7.1 to 4.2 L, subcutaneous adipose tissue (SCAT) by 4.0 L (2.9–7.4 L) from 15.0 to 11.0 L. Log‐transformed rate of change for pancreatic PDFF was moderately correlated with log‐transformed rates for hepatic PDFF, VAT, SCAT, and WC (ρ = 0.5, 0.47, 0.45, and 0.48, respectively), although not statistically significant.Data ConclusionChanges in pancreatic PDFF can be estimated by quantitative CSE‐MRI in adults undergoing a weight‐loss surgery program. Pancreatic and hepatic PDFF and anthropometric indices decreased significantly.Level of Evidence: 2Technical Efficacy Stage: 1J. Magn. Reson. Imaging 2019;50:1092–1102.
Transition zone prostate cancer: Logistic regression and machine‐learning models of quantitative ADC, shape and texture features are highly accurate for diagnosis
Authors: Wu Mark , Krishna Satheesh , Thornhill Rebecca E. , Flood Trevor A. , McInnes Matthew D.F. , Schieda Nicola 
Published: January 2019

BackgroundThe limitation of diagnosis of transition zone (TZ) prostate cancer (PCa) using subjective assessment of multiparametric (mp) MRI with PI‐RADS v2 is related to overlapping features between cancers and stromal benign prostatic hyperplasia (BPH) nodules, particularly in small lesions.PurposeTo evaluate modeling of quantitative apparent diffusion coefficient (ADC), texture, and shape features using logistic regression (LR) and support vector machine (SVM) models for the diagnosis of transition zone PCa.Study TypeRetrospective.PopulationNinety patients; 44 consecutive TZ PCa were compared with 61 consecutive BPH nodules (26 glandular/35 stromal).Field Strength/Sequence3 T/T2‐weighted (T2W) fast spin‐echo, diffusion weighted imaging.AssessmentA radiologist manually segmented lesions on axial images for quantitative ADC (mean, 10th, 25th‐centile‐ADC), T2W‐shape (circularity, convexity) and T2W‐texture (kurtosis, skewness, entropy, run‐length nonuniformity [RLNU], gray‐level nonuniformity [GLNU]) analysis. A second radiologist segmented one‐fifth of randomly selected lesions to determine the reproducibility of measurements. The reference standard was histopathology for all lesions.Statistical TestsQuantitative features were selected a priori and were compared using univariate and multivariate analysis. LR and SVM models of statistically significant features were constructed and evaluated using receiver operator characteristic (ROC) analysis. Subgroup analysis of TZ PCa vs. only stromal BPH and in lesions measuring <15 mm was performed. Agreement in measurements was assessed using the Dice similarity coefficient (DSC).ResultsMean, 25th and 10th‐centile ADC, circularity, and texture (entropy, RLNU, GLNU) features differed between groups (P < 0.0001–0.0058); however, at multivariate analysis only circularity and ADC metrics (P < 0.001) remained significant. LR and SVM models were highly accurate for the diagnosis of TZ PCa (sensitivity/specificity/AUC): 93.2%/98.4%/0.989 and 93.2%/96.7%/0.949, respectively, with no significance difference between the LR and SVM models (P = 0.2271). Reproducibility of segmentation was excellent (DSC 0.84 tumors and 0.87 BPH). Subgroup analyses of TZ PCa vs. stromal BPH (AUC = 0.976) and in <15 mm lesions (AUC = 0.990) remained highly accurate.Data ConclusionLR and SVM models incorporating previously described quantitative ADC, shape and texture analysis features are highly accurate for the diagnosis of TZ PCa and remained accurate when comparing TZ PCa with stromal BPH and in smaller lesions.Level of Evidence: 3Technical Efficacy: Stage 2J. Magn. Reson. Imaging 2019;50:940–950.
Feasibility of a novel mapping system combined with remote magnetic navigation for catheter ablation of premature ventricular contractions
Authors: Dang Shipeng , Jons Christian , Jacobsen Peter Karl , Pehrson Steen , Chen Xu 
Published: January 2019

AbstractBackgroundRemote magnetic navigation (RMN) is often used in combination with a 3‐dimensional mapping system to perform catheter ablations. This study aim to investigate the feasibility and effectiveness of a novel 3D‐mapping system, EnSite Precision, combined with RMN for catheter ablation of premature ventricular contractions (PVCs), and compared it to the procedures performed by CARTO3 with RMN.MethodsForty‐three consecutive PVC patients were either ablated with the guidance of EnSite Precision (n = 22) or CARTO (n = 21) navigated by RMN. Procedure‐related details, acute and long‐term success were assessed.ResultsPatient characteristics between both the groups were similar (age: 47.1 ± 19.8 vs 47.1 ± 12.7, female: 63.6% vs 57.1%). No significant difference was found in the procedure time (99.5 ± 30.4 vs 92.9 ± 24.8 min, P = 0.436), mapping time (18.6 ± 12.8 vs 15.5 ± 10.2 min, P = 0.390), radiofrequency ablation time (333.4 ± 267.0 vs 469.3 ± 343.1 s, P = 0.154), fluoroscopy time (4.0 ± 1.9 vs 3.8 ± 2.0 min, P = 0.635), and X‐ray dose (1.8 ± 1.4 vs 2.0 ± 1.2 Gycm2, P = 0.649) between the two groups. No significant procedural complication occurred in either group. In addition, there was no significant differences regarding the acute success rate (90.9% vs 90.5%, P = 0.961) and long‐term success rate (86.4% vs 81.0%, P = 0.631) after 16.2 ± 6.2 months of follow‐up between the two groups.ConclusionsRMN combined with EnSite Precision mapping system is effective and safe for catheter ablation of PVCs.
Are men better negotiators everywhere? A meta‐analysis of how gender differences in negotiation performance vary across cultures
Authors: Shan Wen , Keller Joshua , Joseph Damien 
Published: January 2019

SummaryPrior research has found that each culture and gender influences negotiation strategies and outcomes, but less is known about their interplay. We integrate these two research streams by providing a meta‐analytic review of the interactive impact of gender and culture on negotiation performance. We reviewed 185 studies that reported gender differences in intracultural negotiation performance across 30 societies that varied across seven cultural dimensions. Results showed that Hofstede's individualism–collectivism, GLOBE's in‐group collectivism and assertiveness practices, and Schwartz's harmony moderated the gender effect on negotiation performance. We found that in cultures lower in individualism, higher in in‐group collectivism practices, lower in assertiveness practices, and higher in harmony, women more likely outperformed men in negotiations. Implications for the role of gender and culture in negotiations and organizations more broadly are discussed.
Effect of guided bone regeneration on immediately placed implants: Meta‐analyses with at least 12 months follow‐up after functional loading
Authors: Kinaia Bassam M. , Kazerani Shahrdad , Korkis Samuel , Masabni Omar Mario , Shah Maanas , Neely Anthony L. 
Published: January 2019

AbstractBackgroundImmediate implant placement (IIP) into fresh extraction socket is a favorable treatment option. If successfully managed, it reduces the overall treatment time, and increases patient's satisfaction. Surgical and restorative factors affect IIP success rates. In this systematic review we evaluate the effect of guided bone regeneration (GBR) at the time of IIP on crestal bone level (CBL) changes after at least 12‐months of functional loading.MethodsReviewers conducted an independent search of the National Center for Biotechnology Information PubMed, Medline, and the Cochrane Collaboration Library from 1966 to January 2017 following the inclusion criteria. A hand search of bibliographies of reviews and clinical trials related to IIP was also performed. This study looked into CBL changes around IIP primarily and further extracted the data to conduct three meta‐analysis of “IIP using GBR versus IIP without GBR”, “IIP using bone graft alone versus IIP using bone graft with membrane” and “IIP using GBR versus conventional implant placement” which were further subdivided to provide more detailed information for each. Four reviewers independently assessed the study data and methodologic quality using data extraction and assessment forms.ResultsThe electronic search identified 714 potential studies and the hand search retrieved 55 studies. Crestal bone level (CBL) changes were determined in three meta‐analyses. The results revealed a mean difference in CBL changes of 0.175 ± 0.180 mm in favor of IIP without GBR when compared with implant with GBR. However, IIP with bone graft and membrane showed better results when compared with IIP with bone graft alone [CBL changes of 0.532 ± 0.572 mm]. CBL preservation was noted in IIP with GBR versus conventional implant placement [CBL changes of – 0.001 ± 0.049 mm].ConclusionsMeta‐analyses showed minimal difference in CBL around IIP with bone graft versus without bone graft and with IIP with GBR compared with conventional implant placement. However, IIP with bone graft and membrane reported better CBL preservation compared with IIP with bone graft alone. Nonetheless, these results should be interpreted with caution because of moderate heterogeneity between studies.
Information integration of force sensing and machine vision for in‐shell shrivelled walnut detection based on the golden‐section search optimal discrimination threshold
Authors: Zhai Zhiqiang , Jin Zuohui , Zhang Ruoyu 
Published: January 2019

AbstractBACKGROUNDThe shrivelled defect of walnuts has a serious effect on walnut quality and is a common internal defect of in‐shell walnuts. However, only depending on a single detection technique such as machine vision to detect in‐shell shrivelled walnuts is challenging. Meanwhile, the threshold has a great impact on the accuracy of the discrimination analysis. Therefore, the golden‐section was used to search the optimal discrimination threshold and the information integration of force sensing and machine vision was used to identify the shrivelled walnut and the sound walnut.RESULTSA discrimination model for in‐shell shrivelled walnut based on information integration of force sensing and machine vision was built. The optimal threshold was determined as 0.3464 by the golden‐section method, and the optimal threshold was used to discriminate the in‐shell shrivelled walnut and the sound walnut. The discriminant accuracy of in‐shell shrivelled and sound walnuts were 96.97% and 85.29%, respectively, and the total discriminant accuracy reached 93.00% based on the discrimination model.CONCLUSIONThe results indicated the information integration of force sensing and machine vision based on the golden‐section search optimal discrimination threshold is a potential method to discriminate shrivelled walnuts and sound walnuts, which also makes a basis for on‐line detection of in‐shell shrivelled walnut. © 2019 Society of Chemical Industry
Determination of estrone and 17α‐ethinylestradiol in digested sludge by ultrasonic liquid extraction and high‐performance liquid chromatography with fluorescence detection
Authors: Louros Vitória L. , Lima Diana L.D. , Leitão Jorge H. , Esteves Valdemar I. , Nadais Helena G. 
Published: January 2019

AbstractEstrone, 17β‐estradiol and 17α‐ethinylestradiol are increasingly recognised as important micropollutants to be monitored in wastewater treatment plants. These estrogens are retained onto sludge due to their high adsorption and since they are largely used in land applications, the monitoring of these chemicals in sludge samples is of great importance. This study describes a method for the determination of estrone and 17α‐ethinylestradiol in fresh sludge samples. After spiking fresh digested sludge with estrone and 17α‐ethinylestradiol and maintaining in contact during 5, 30 and 60 min, the freeze‐dried samples were subjected to ultrasonic liquid extraction, with methanol and acetone, and analysed by high‐performance liquid chromatography with fluorescence detection. The average recoveries obtained for estrone and 17α‐ethinylestradiol using the different contact times were 103 ± 3 and 97 ± 4%, respectively. Fresh sludge samples from one waste water treatment plant located in Portugal were analysed and estrone was detected in primary fresh sludge, anaerobic digested sludge and dehydrated sludge at a concentration in the range of 1–4.8 μg/g. The method here developed does not require any sample clean‐up, being fast and simple, reliable and inexpensive, making possible its application for monitoring the contamination of sludge with these estrogens.
Investigation of dynamic accumulation and regularity of nine glycosides and saccharides in Rehmannia glutinosa by rapid quantitative analysis technology
Authors: Xu Zhuo , Dai Xinxin , Su Shulan , Yan Hui , Guo Sheng , Qian Dawei , Duan Jin‐ao 
Published: January 2019

AbstractIn the process of planting and harvesting of Rehmannia glutinosa, only the underground part is used, and a large number of stems and leaves that are considered non‐medicinal parts are usually discarded. Recent studies have shown that the chemical components in the leaves are similar to those identified in the roots. In this study, we selected leaves and roots from Rehmannia glutinosa at different growth stages and leaves from different cultivation regions to investigate the dynamic accumulation of three kinds of glycosides (catalpol, acteoside, and ajugol), six kinds of carbohydrates (rhamnose, fructose, sucrose, melibiose, stachyose, and verbascose), and acidic and neutral polysaccharides via rapid quantitative analysis technology, including ultra high performance liquid chromatography triple quadrupole tandem mass spectrometry, high‐performance liquid chromatography, and UV spectroscopy. The results showed that the Rehmannia glutinosa leaves also contained higher content of catalpol (3.81∼24.51 mg/g), ajugol (0.55∼10.23 mg/g), acteoside (1.34∼21.16 mg/g), monosaccharide/ oligosaccharides (7.71∼120.73 mg/g), and polysaccharides (5.63∼15.57%). In this study, we developed a new rapid and simple method for determination to clarify the distribution and dynamic accumulation of nine glycosides and saccharides in Rehmannia glutinosa leaves to provide a scientific basis for the discovery, development, and utilization of the resource value of Rehmannia glutinosa leaves.