bluuebunny/crossref_metadata_2025_split huggingface dataset:
Catalytic Formation of C−N Bonds: ICS Symposium Honoring Wolf Prize Laureates Stephen L. Buchwald and John F. Hartwig: May 29, 2019, Technion‐Israel Institute of Technology, Haifa, Israel
Authors: Keinan Ehud 
Published: September 2019

AbstractThis report covers two exciting events in the scientific landscape of the State of Israel: the traditional Wolf Prize Symposium of the ICS and the Wolf Prize Ceremony in the Knesset. The symposium, dedicated to the science of Stephen L. Buchwald and John F. Hartwig, highlighted the catalytic formation of C−N bonds. In a general sense, the two Wolf Prize laureates may be considered as molecular architects who produced efficient molecular‐scale machines that make important molecules for the benefit of humanity. After receiving the Wolf Prize from Israel's President, Buchwald commented, “There are many who believe that support for research should focus exclusively on endeavors that have specific practical applications in mind. With this mindset, our work would have never been possible. Time and time again experience shows that it is exceedingly difficult to predict which scientific discoveries will lead to major advances. So often, it is the scientist following his or her own intellectual curiosity whose work leads to a breakthrough. I believe that basic curiosity‐driven research and societal and economic progress are inextricably linked.” And Hartwig comments, “We all know the principles of science know no boundaries, but maybe less appreciated, or taken for granted, is that the assembly of research teams in many places knows no boundaries. If we recognize and nurture talent in people from all corners and all backgrounds we can address and maybe solve today's most important problems in health, energy, and environmental sustainability that are urgently facing us.”
Association between person‐centered maternity care and newborn complications in Kenya
Authors: Sudhinaraset May , Landrian Amanda , Afulani Patience A. , Diamond‐Smith Nadia , Golub Ginger 
Published: September 2019

AbstractObjectiveDespite the recognized importance of person‐centered care, very little information exists on how person‐centered maternity care (PCMC) impacts newborn health.MethodsBaseline and follow‐up data were collected from women who delivered in government health facilities in Nairobi and Kiambu counties in Kenya between August 2016 and February 2017. The final analytic sample included 413 respondents who completed the baseline survey and at least one follow‐up survey at 2, 6, 8, and/or 10 weeks. Data were analyzed using descriptive, bivariate, and multivariate statistics. Logistic regression was used to assess the relationship between PCMC scores and outcomes of interest.ResultsIn multivariate analyses, women with high PCMC scores were significantly less likely to report newborn complications than women with low PCMC scores (adjusted odds ratio [aOR] 0.39, 95% confidence interval [CI] 0.16–0.98). Women reporting high PCMC scores also had significantly higher odds of reporting a willingness to return to the facility for their next delivery than women with low PCMC score (aOR 12.72, 95% CI 2.26–71.63). The domains of Respect/Dignity and Supportive Care were associated with fewer newborn complications and willingness to return to a facility.ConclusionPCMC could improve not just the experience of the mother during childbirth, but also the health of her newborn and future health‐seeking behavior.
Development of a cross‐validated model for predicting emergency cesarean for intrapartum fetal compromise at term
Authors: Flatley Christopher , Gibbons Kristen Stacey , Hurst Cameron , Kumar Sailesh 
Published: September 2019

AbstractObjectivesTo develop a model for predicting emergency cesarean for fetal distress (ECFD) at term using a combination of maternal and late pregnancy ultrasound parameters measured at more than 36 gestational weeks.MethodsA study of prospectively collected data, including ultrasound scans at 36–38 weeks, for singleton non‐anomalous deliveries at Mater Mother's Hospital, Brisbane, Australia, between January 2010 and April 2017. Univariable and multivariable mixed‐effects generalized linear models were generated. The final model was validated by the K‐fold cross validation technique.ResultsOverall, 5439 women met the inclusion criteria; of these, 230 (4.2%) underwent ECFD. There were more nulliparous women and women with induction of labor (IOL) in the ECFD cohort (both P < 0.001). ECFD neonates had lower z‐scores for estimated fetal weight (EFW), cerebroplacental ratio (CPR), and middle cerebral artery pulsatility index; and higher scores for umbilical artery pulsatility index. Ethnicity, nulliparity, IOL, EFW z‐score, and CPR z‐score were included in the final prediction model, which showed high accuracy with an area under the receiver operator characteristic curve of 0.77.ConclusionThe study shows that a prediction model combining the continuous standardized measures of CPR and EFW and several maternal factors was able to identify ECFD with improved accuracy.
LINC00365 promotes colorectal cancer cell progression through the Wnt/β‐catenin signaling pathway
Authors: Zhu Yiping , Bian Yinzhu , Zhang Qun , Hu Jing , Li Li , Yang Mi , Qian Hanqing , Yu Lixia , Liu Baorui , Qian Xiaoping 
Published: September 2019

AbstractIn the past decade, substantial evidence established that long noncoding RNAs are serious about mediating the evolution of malignancies. In previous studies, LINC00365, which has not been reported in colorectal cancer (CRC), was selected using the bioinformatics analysis in GSE109454 and GSE41655 data sets. However, the function and mechanism of LINC00365 are still obscure. In our study, LINC00365 was found upregulated in CRC specimens and intimately connected with the prognosis of patients with CRC. In addition, LINC00365 overexpression enhances the cell abilities of proliferation, migration, and invasion in vitro. Meanwhile, mechanistic studies showed that LINC00365 might involve in CRC cell progression by mediating the Wnt/β‐catenin pathway. Furthermore, LINC00365 upregulation increased CDK1 protein expression. In conclusion, this study suggests that LINC00365 acts as a vital part in facilitating CRC progression and might play as a therapeutic target for patients with CRC.
MicroRNA‐203 diminishes the stemness of human colon cancer cells by suppressing GATA6 expression
Authors: Lai Hung‐Tzi , Tseng Wen‐Ko , Huang Shi‐Wei , Chao Ta‐Chung , Su Yeu 
Published: September 2019

AbstractThe interaction between hyaluronan and CD44, an important cancer stem‐cell marker, stimulates various tumor cell‐specific functions such as the stemness of tumor cells. microRNA‐203 (miR‐203) can be downregulated by this interaction in human colorectal cancer (CRC) cells, which increases their stemness; however, the underlying mechanism is not yet defined. Here, we show that overexpression and sequestration of miR‐203 in HCT‐116 and HT‐29 human CRC cells reduces and enhances their stemness, respectively. We also show that GATA‐binding factor 6 (GATA6) is a direct target of miR‐203. Our results indicate that upregulated expression of this transcription factor not only restores the self‐renewal abilities of miR‐203‐overexpressing HCT‐116 and HT‐29 cells but also promotes the stemness properties of their parental counterparts. More important, we show that silencing the expression of either LRH‐1 or Hes‐1 is sufficient to diminish the stemness‐promoting effects of GATA6 in human CRC cells. Together, our findings delineate the stemness‐inhibitory mechanism of miR‐203 in human CRC cells and suggest that this miR is a potential therapeutic agent for colorectal cancer.
miR‐204‐5p regulates cell proliferation, invasion, and apoptosis by targeting IL‐11 in esophageal squamous cell carcinoma
Authors: Tang Jianwei , Li Zhihua , Zhu Quan , Wen Wei , Wang Jun , Xu Jing , Wu Weibin , Zhu Yining , Xu Honglei , Chen Liang 
Published: September 2019

AbstractEsophageal cancer (EC) is the world's eighth most common malignant neoplasm and is ranked as the sixth leading cause of death related to cancer. Aberrant microRNA (miRNA) expression has been reported to be associated with esophageal squamous cell carcinoma. However, the molecular mechanism of miR‐204‐5p in esophageal squamous cell carcinoma (ESCC) is not clear. Therefore, the aim of this study was to investigate the potential role of miR‐204‐5p in ESCC. In the present study, we found that miR‐204‐5p could affect ESCC proliferation, invasion, apoptosis, and cell cycle in cell and mouse models. A dual‐luciferase reporter assay showed that miR‐204‐5p expression was negatively correlated with interleukin‐11 (IL‐11) expression. IL‐11 overexpression reversed the suppressive effects of miR‐204‐5p in the cell lines. These results indicated that miR‐204‐5p functions as a tumor suppressor by directly targeting IL‐11 in ESCC.
Virulence and DNA fingerprinting analysis of Xanthomonas oryzae pv. oryzae identify a new pathotype in Guangxi, South China
Authors: Chen Xiaolin , Wei Shanfu , Yan Qun , Huang Fengkuan , Ma Zengfeng , Li Ruifang , Cen Zhenlu , Yan Weihong , Li Kunhua 
Published: September 2019

AbstractBacterial blight caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the most destructive diseases affecting rice worldwide. However, little is known about the population structure of this organism in Guangxi Zhuang Autonomous Region, South China. Here, pathotypic and DNA fingerprint analyses were conducted to characterize the isolates of Xoo collected from rice leaves in five districts of the region from 2013 to 2016. Their pathogenicity was tested by leaf clipping, and the DNA fingerprints were analyzed by repetitive sequence‐based polymerase chain reaction and endogenous insertion sequence element‐based polymerase chain reaction assays using the repetitive extragenic palindromic sequence and enterobacterial repetitive intergenic consensus primers, respectively. Pathogenicity assays of 70 representative isolates were conducted using a series of near‐isogenic lines and two new pathotypes were identified. All the pathotypes were found to be incompatible with xa5 and Xa7. One pathotype was virulent to Xa14, Xa21, and Xa23, whereas another virulent to Xa21 and Xa23, but incompatible with Xa14. A dendrogram generated for the data sets obtained from DNA fingerprinting suggested the prevalence of high genetic diversity of Xoo throughout Guangxi, and no association between the molecular haplotypes and pathotypes was identified.
LncRNA DANCR promotes tumor growth and angiogenesis in ovarian cancer through direct targeting of miR‐145
Authors: Lin Xiaojuan , Yang Fan , Qi Xiaorong , Li Qingli , Wang Danqing , Yi Tao , Yin Rutie , Zhao Xia , Zhong Xiaomin , Bian Ce 
Published: September 2019

AbstractDifferentiation antagonizing non‐protein coding RNA (DANCR) is a newly identified oncogenic long noncoding RNA found in various cancers. However, the functional role of DANCR in tumor angiogenesis and the underlying mechanisms are still unclear. The expression of DANCR was determined in ovarian malignant tissues and cell lines. The functional role of DANCR in tumor angiogenesis was revealed by the following methods: CD31 staining of ovarian tumor tissues, matrigel‐plug assay tissues, HUVEC‐related tube formation assay, and invasion assay. Enzyme‐linked immunosorbent assay, Western blotting, luciferase assay, and rescue experiments were used to investigate the underlying mechanisms of DANCR‐regulating angiogenesis. DANCR was upregulated in ovarian malignant tissues and ovarian cancer cells. Knockdown of DANCR efficiently impaired ovarian tumor growth through inhibition of tumor angiogenesis. Furthermore, the conditional culture medium from DANCR‐knockdown ovarian cells significantly inhibited tube formation and invasion of HUVEC in vitro. Mechanistic investigation indicated that vascular endothelial growth factor A (VEGF‐A, VEGF) plays a crucial role during DANCR inhibition of tumor angiogenesis in ovarian cancer. Further results demonstrated that miR‐145 is the direct binding target of DANCR during regulation of VEGF expression and tumor angiogenesis in ovarian cancer cells. Collectively, DANCR plays a promotional role in tumor angiogenesis in ovarian cancer through regulation of miR‐145/VEGF axis. Therefore, DANCR may be a novel therapy target for ovarian cancer.
XRCC1‐mediated DNA repair is associated with progression‐free survival of multiple myeloma patients after autologous stem cell transplant
Authors: Persaud Avinash K. , Li Junan , Johnson Jasmine A. , Seligson Nathan , Sborov Douglas W. , Duah Ernest , Cho Yu Kyoung , Wang Danxin , Phelps Mitch A. , Hofmeister Craig C. , Poi Ming J. 
Published: September 2019

AbstractAutologous stem cell transplant (ASCT) with high‐dose melphalan (HDM) is the standard treatment for fit multiple myeloma (MM) patients. It is generally believed that some DNA repair proteins impact the activity to repair melphalan‐induced DNA damage, thus potentially contributing to the patient's clinical response. However, knowledge of these proteins is limited. In the current study, we investigated the roles of XRCC1, a protein involved in base excision repair and single‐strand break repair, in melphalan response in MM cells. Small interfering RNA knockdown of XRCC1 significantly increased the accumulation of melphalan‐induced DNA damage in MM cells and sensitized them to melphalan treatment, indicating that genetic variation in XRCC1 may impact response to melphalan treatment. We then evaluated the association between an XRCC1 variant with reduced activity, rs25487 (R399Q), and clinical outcomes of 108 MM patients with melphalan therapy. Our results showed that XRCC1 rs25487 was associated with prolonged progression‐free survival (PFS) in MM patients. The adjusted hazard ratio for PFS between patients carrying rs25487 AA/AG and GG was 0.42 (95% confidence interval: 0.25, 0.84, P = .014). Taken together, these results indicate that XRCC1 is involved in the repair of melphalan‐induced DNA damage and XRCC1 rs25487 variant with impaired DNA repair function influences the clinical responses of HDM in MM patients.
Maternal transcripts in good and poor quality eggs from Japanese eel, Anguilla japonica—their identification by large‐scale quantitative analysis
Authors: Izumi Hikari , Gen Koichiro , Lokman P. Mark , Hagihara Seishi , Horiuchi Moemi , Tanaka Toshiomi , Ijiri Shigeho , Adachi Shinji 
Published: September 2019

AbstractOur understanding of maternal control of development in vertebrates remains incomplete. In this study, we investigated levels of maternal transcripts in good and poor quality eggs from artificially matured Japanese eel, using RNA‐Seq and quantitative polymerase chain reaction (qPCR), to identify candidate maternal transcripts related to development. De novo assembly or mapping of reads to the eel draft genome yielded 619,029 contigs and 85,906 transcripts, respectively; normalized read counts to these assemblies were calculated using reads (RPKM) or fragments (FPKM) per kilobase of transcript per million mapped reads. In silico screening identified 1,594 contigs and 150 transcripts with lower RPKM or FPKM in poor than in good quality eggs, 245 contigs, and 85 transcripts of which could be annotated by BLASTx, respectively. From selected contigs or transcripts, six genes (dnajb4, gnpat, card14, pdp1, fcgbp, ttn) had significantly lower messenger RNA levels in poor than in good quality eggs by qPCR. Multiple regression analysis showed that five genes (gnpat, b4galnt1, acsl6, rtkn, trim24) significantly correlated with hatchability. Taken together, 10 genes were identified as candidate maternal transcripts, regulating development in Japanese eel. Our results contribute to understanding the molecular basis for maternal control of development in vertebrates.
Impact of Ice-Angling and Handling on Swimming Performance in Bluegill and Largemouth Bass
Authors: Bieber John F. , Louison Michael J. , Stein Jeffrey A. , Suski Cory D. 
Published: September 2019

Abstract
Many recreational anglers practice catch-and-release; however, research indicates that capture and handling has the potential to adversely affect fish. Numerous catch-and-release studies have been conducted during warmer months, but little work has been done during the winter when ice-anglers in temperate regions target fish. We conducted an ice angling simulation that quantified the impacts of air temperature and air exposure duration on swimming performance and gill physiology of Bluegill Lepomis macrochirus and Largemouth Bass Micropterus salmoides. In all experiments, fish were first subjected to a simulated angling bout in water at 5°C, followed by 30 s or 5 min of air exposure at above freezing (3–8°C) or subfreezing (−7°C) temperatures. The fish were then assessed for critical swimming speed (Bluegill), oxygen consumption (Bluegill), burst swimming (Largemouth Bass), or gill damage (Largemouth Bass). Results showed that Bluegill subjected to 5 min of air exposure at −7°C suffered impaired swimming, with a 47% loss in critical swimming speed (Ucrit) compared with the controls. Treatment had no impact on burst swimming or gill damage in Largemouth Bass. The results demonstrate the possible impacts of air exposure on fish, and we recommend that ice-anglers make an effort to minimize air exposure duration, especially when air temperatures are low.
A Protocol to Deliver Intensive Behavioral Therapy (IBT) for Obesity in Primary Care Settings: The MODEL‐IBT Program
Authors: Wadden Thomas A. , Tsai Adam G. , Tronieri Jena Shaw 
Published: September 2019

ObjectiveThe Centers for Medicare and Medicaid Services (CMS) initiated coverage of intensive behavioral therapy (IBT) for obesity in 2011, providing beneficiaries 14 to 15 brief, individual counseling visits in 6 months. CMS offered general recommendations for delivering IBT but did not provide an evidence‐based treatment protocol, which was the objective of the present research.MethodsThis review describes the evidence that CMS considered in developing its IBT benefit. It also examines weight losses produced by the intensive lifestyle intervention in the Diabetes Prevention Program (DPP), as well an adapted version of the DPP delivered (for the first 6 months) on the visit schedule recommended by CMS. This new protocol, which was evaluated in a recent randomized trial, provided 14 visits in the first 24 weeks, with 7 additional monthly visits through week 52.ResultsAs reported previously, the 50 participants with obesity assigned to the new IBT protocol lost a mean of 5.4% of their initial weight at week 24; 46% of participants lost ≥ 5% of their baseline weight. At 1 year, the mean loss was 6.1%, and 44% of participants lost ≥ 5%.ConclusionsWith these generally favorable results, the IBT protocol is being posted online for practitioners and researchers to use.
Lipedema: A Call to Action!
Authors: Buso Giacomo , Depairon Michele , Tomson Didier , Raffoul Wassim , Vettor Roberto , Mazzolai Lucia 
Published: September 2019

Lipedema is a chronic progressive disease characterized by abnormal fat distribution resulting in disproportionate, painful limbs. It almost exclusively affects women, leading to considerable disability, daily functioning impairment, and psychosocial distress. Literature shows both scarce and conflicting data regarding its prevalence. Lipedema has been considered a rare entity by several authors, though it may be a far more frequent condition than thought. Despite the clinical impact on women's health, lipedema is in fact mostly unknown, underdiagnosed, and too often misdiagnosed with other similarly presenting diseases. Polygenic susceptibility combined with hormonal, microvascular, and lymphatic disorders may be partly responsible for its development. Furthermore, consistent information on lipedema pathophysiology is still lacking, and an etiological treatment is not yet available. Weight loss measures exhibit minimal effect on the abnormal body fat distribution, resulting in eating disorders, increased obesity risk, depression, and other psychological complaints. Surgical techniques, such as liposuction and excisional lipectomy, represent therapeutic options in selected cases. This review aims to outline current evidence regarding lipedema epidemiology, pathophysiology, clinical presentation, differential diagnosis, and management. Increased awareness and a better understanding of its clinical presentation and pathophysiology are warranted to enable clinicians to diagnose and treat affected patients at an earlier stage.
Strategy to minimize radiation burden in infants and high‐risk medulloblastoma using intrathecal methotrexate and high‐dose chemotherapy: A prospective registry study in Japan
Authors: Yamasaki Kai , Okada Keiko , Soejima Toshinori , Sakamoto Hiroaki , Hara Junichi 
Published: September 2019

AbstractBackgroundMost childhood medulloblastoma (MB) cases are curable using multimodal treatment, including craniospinal irradiation (CSI). However, late effects are a serious problem for survivors. This prospective registry study evaluated Japanese patients to determine whether a reduced radiation dose was feasible.Patients and MethodsPatients with MB were classified as an infant group (<3 years old) and a high‐risk (HR) group (≥3 years old with metastasis). The HR group received intrathecal methotrexate (IT‐MTX) and high‐dose chemotherapy (HDC) using thiotepa and melphalan, as well as concomitant radiotherapy with a recommended CSI dose of 18 Gy and a total local dose of 50 Gy. Radiotherapy was only considered for infants if residual tumors were present after the HDC.ResultsBetween 1997 and 2006, we identified 28 HR patients (M1: 9, M2/3: 19) and 17 infant patients (M0: 11, M1: 3, M2/3: 3). During the median follow‐up of 9.4 years for the entire HR group, the 5‐year progression‐free survival (PFS) rate was 82.1 ± 7.2% and the 5‐year overall survival (OS) rate was 85.7 ± 6.6%. Subanalyses of the patients who received the recommended treatment revealed that the 5‐year PFS and OS rates were both 90.5 ± 6.4%. In the infant group, the 5‐year PFS rate was 52.9 ± 12.1% and the 5‐year OS rate was 51.8 ± 12.4%. There were no serious adverse events associated with the IT‐MTX and HDC treatments.ConclusionIntensified chemotherapy using HDC and IT‐MTX might allow for a reduced prophylactic radiation dose in patients with MB with metastases. Further studies are needed to validate these findings.
Conformity to masculinity ideology, a predictor of academic‐related attitudes and behaviors
Authors: Liang Christopher T. H. , Rocchino Gabrielle H. , Gutekunst Malaïka H. C. , Smithson Annalisa 
Published: September 2019

AbstractMasculinity ideology is argued to be associated with academic outcomes. However, relatively few studies have examined these associations. This study examines the associations between masculinity ideology and academic skepticism, self‐presentation of low achievement, academic engagement, academic initiative, and self‐regulation, above and beyond academic self‐efficacy, among a diverse sample of urban youth. With a sample of 135 eighth‐grade students from a Northeastern middle school, 5 separate multiple hierarchical regression analyses were used to examine whether students’ endorsement of masculinity ideology predicted the criterion variables above and beyond academic self‐efficacy. The sample was comprised of 54 girls and 74 boys ages 12–14 years (M = 13.14; SD = .37). Results showed academic self‐efficacy significantly predicted academic engagement (B = 1.26; β = .53, p < .001; f2 = .39), academic initiative (B = .98; β = .37; p < .001, f2 = .16), and behavioral regulation (B = .61; β = .24; p < .05; f2 = .09) and explained an additional 8 and 10% of the variance in self‐presentation of low achievement (B = .54; β = .33; p = .001; f2 = .14) and academic skepticism (B = .56; β = .35; p < .001; f2 = .15), respectively, over and above academic self‐efficacy. Gender, which was dummy coded and entered as a covariate, only predicted self‐presentation of low achievement. Findings from this study point to the need to foster positive academic outcomes through bolstering academic self‐efficacy and, perhaps more important, addressing masculinity ideology.