bluuebunny/crossref_metadata_2025_split huggingface dataset:
Specification of diverse cell types during early neurogenesis of the mouse cerebellum
Authors: Wizeman John W. , Guo Qiuxia , Wilion Elliot , Li James Y.H. 
Published: October 2018

SUMMARYWe applied single-cell RNA sequencing to profile genome-wide gene expression in about 9,400 individual cerebellar cells from the mouse embryo at embryonic day 13.5. Reiterative clustering identified the major cerebellar cell types and subpopulations of different lineages. Through pseudotemporal ordering to reconstruct developmental trajectories, we identified novel transcriptional programs controlling cell fate specification of populations arising from the ventricular zone and the anterior rhombic lip, two distinct germinal zones of the embryonic cerebellum. Together, our data revealed cell-specific markers for studying the cerebellum, important specification decisions, and a number of previously unknown subpopulations that may play an integral role in the formation and function of the cerebellum. Importantly, we identified a potential mechanism of vermis formation, which is affected by multiple congenital cerebellar defects. Our findings will facilitate new discovery by providing insights into the molecular and cell type diversity in the developing cerebellum.
Calotropis gigantea extract induces apoptosis through extrinsic/intrinsic pathways and upregulation of reactive oxygen species in non-small-cell lung cancer cells
Authors: Lee Jiyon , Jang Huiju , Bak Yesol , Shin Jong-Woon , Jin Hang , Kim Yong-In , Won Ryu Hyung , Ryang Oh Sei , Yoon Do-Young 
Published: October 2018

AbstractBackgroundCalotropis gigantea (CG) plant grows in Asia and tropical Africa. However, the precise mechanisms of its anticancer effects have not yet been examined in human non-small cell lung cancer (NSCLC) cells, A549 and NCI-H1299 cells.PurposeThis study was focused on the anti-cancer effects of CG extract on non-small cell lung cancer (NSCLC) cells.MethodsThe cytotoxic effects of CG extract on NSCLC, A549 and NCI-H1299 cells, were detected by MTS assay, microscope and DAPI staining. Apoptosis was determined by annexin V-FITC/PI staining, cell cycle analysis, western blotting, quantitative polymerase chain reaction, and JC-1 staining.ResultsFirst, CG showed significant dose-dependent cytotoxicity in NSCLC, A549, and NCI-H1299 cells. In addition to induction of caspase-8 processing, CG induced apoptosis by upregulating mRNA expression levels of extrinsic pathway molecules such as Fas, Fas ligand (FasL), Fas-associated protein with death domain (FADD) and death receptor 5 (DR5). Also, mitochondrial membrane potential (MMP) was collapsed, and intrinsic pathway molecules such as poly (ADP-ribose) polymerase (PARP), caspase-3, and caspase-9 were processed by CG. Moreover, reactive oxygen species (ROS) were generated in a CG dose-dependent manner, and inhibition of ROS by NAC, ROS scavenger, recovered A549 and NCI-H1299 cell viability.ConclusionThese results indicate that CG causes apoptosis by activating the extrinsic and intrinsic pathways and generating ROS in NSCLC cells. These results suggest that CG can be used as a lung cancer therapeutic agent.
Thymic Stromal Lymphopoietin Promotes Proliferation and Contractility of Human Pulmonary Artery Smooth Muscle
Authors: Thompson Michael , Sathish Venkatachalem , Manlove Logan , Roos Benjamin , Wang Bowen , Vassallo Robert , Miller Jordan , Pabelick Christina M. , Prakash Y.S. 
Published: October 2018

AbstractHypoxia is a well-recognized risk factor in several pulmonary vascular diseases including pulmonary hypertension (PH). Furthermore, hypoxia-associated inflammatory changes enhance the structural and functional changes in the pulmonary artery (PA) of PH patients. Understanding the mechanisms that link hypoxia and inflammation, particularly early in disease, is key to development of novel therapeutic avenues for PH. Thymic stromal lymphopoietin (TSLP) is an “early” inflammatory mediator thought to be critical in diseases such as asthma, chronic obstructive pulmonary disease and atopic dermatitis. TSLP has canonical effects on the immune system, but can also have non-canonical effects on resident lung cells, e.g. airway smooth muscle. Currently, the expression and role of TSLP in the PA is unknown. We hypothesized that locally-produced TSLP potentiates the effects of hypoxia in PA remodeling and contractility relevant to PH. Experiments in human PA endothelial cells (PAECs) and smooth muscle cells (PASMCs) found PAECs to be a larger source of TSLP which targets PASMCs to enhance intracellular Ca2+responses to vasoconstrictor agonist as well as cell proliferation, acting via a number of signaling cascades including Stat3 and PI3/Akt. Hypoxia, acting via HIF1α, enhanced PAEC production of TSLP, and promoted TSLP effects on PASMCs. Interestingly, TSLP per se enhance HIF1a. Overall, these novel data highlight a role for TSLP in hypoxia effects on the PA, and thus relevance for inflammation in PH.
Geoelectrical study of corrosivity and competence of soils within Uburu and Okposi areas of Ebonyi State, Southeastern Nigeria
Authors: Ekwe Amobi C. , Opara Alexander I. , Onwuka Obialo S. 
Published: October 2018

Purpose
The corrosivity and competence of soils within Uburu and Okposi areas of the Southern Benue Trough, Nigeria, were evaluated using the electrical resistivity method. This paper aims to provide information that will aid pre-design of subsurface iron/steel pipe networks for distribution of pipe-borne water and construction of subsurface structures for agricultural and environmental purposes.

Design/methodology/approach
In total, 22 vertical electrical soundings (VES) in the Schlumberger configuration were acquired with Allied Ohmega™ Terrameter with a maximum half current (AB/2) electrode spacing of 200 m. Layer parameters were determined using partial curve matching techniques, using the Schlumberger master curves, while processing and modelling were done with the IPI2win™ software. The VES results were interpreted qualitatively and quantitatively to obtain various curve types and layer parameters, respectively, which were used to categorize the area into different competence and corrosivity zones. The first layer isoresistivity and competence maps were used to delineate four zones (A,B,C and D) with varying apparent resistivities and competences.

Findings
Incompetent soils with resistivity values ranging from 24.3-88.7 Om are found in Zone A. The soils in Zone A are mainly expansive clays which swell on absorption of water. Zone B contains moderately competent soils with resistivity values ranging from 273-308.6 Om, while Zones C and D are underlain by sandstones and contain competent to highly competent soils with resistivity values ranging from 511-750 Om and 835-1,525 Om, respectively. Zone E contains highly corrosive (24.3 Om) to mildly corrosive (102 Om) soils; Zone F contains soils that are essentially non-corrosive with resistivity values ranging from 271-1,525 Om, while the corrosivity of soils within Zone G varies from corrosive to mildly corrosive, with resistivity values ranging from 44.3-114 Om.

Research limitations/implications
Some of the areas are not accessible because of community restrictions.

Practical implications
These findings are essentially very significant and should be taken into consideration when materials that are susceptible to corrosion are being considered for engineering, agricultural and environmental purposes in the area.

Social implications
The findings will aid water resource planners and developers on how to protect metal pipes from corrosion, when used for water reticulation and agricultural purposes.

Originality/value
This paper fulfils an identified need to study the corrosivity of soils in the study area with a view to providing adequate protection to metal objects when being considered for water reticulation for domestic and agricultural purposes in the area.
Bio-inspired aircraft control
Authors: Zikmund Pavel , Macík Miroslav , Dvořák Petr , Míkovec Zdeněk 
Published: October 2018

PurposeThis paper aims to present a state-of-the-art review in various fields of interest, leading to a new concept of bio-inspired control of small aircraft. The main goal is to improve controllability and safety in flying at low speeds.Design/methodology/approachThe review part of the paper gives an overview of artificial and natural flow sensors and haptic feedback actuators and applications. This background leads to a discussion part where the topics are synthesized and the trend in control of small aircraft is estimated.FindingsThe gap in recent aircraft control is identified in the pilot–aircraft interaction. A pilot’s sensory load is discussed and several recommendations for improved control system architecture are laid out in the paper.Practical implicationsThe paper points out an opportunity for a following research of suggested bio-inspired aircraft control. The control is based on the artificial feeling of aerodynamic forces acting on a wing by means of haptic feedback.Originality/valueThe paper merges two research fields – aircraft control and human–machine interaction. This combination reveals new possibilities of aircraft control.
Application of computed tomography to hole expansion measurements
Authors: Wronicz Wojciech , Malicki Maciej 
Published: October 2018

Purpose
The purpose of this paper is to investigate a possibility of determination of the rivet hole expansion with the use of computed tomography (CT). This method offers several benefits in comparison to the traditionally used destructive methods.

Design/methodology/approach
The measurements of rivet hole expansion were performed on three specimens with the use of CT. Then, the same specimens were measured with the use of the conventional destructive method. This allows to estimate accuracy of the proposed method and characterize its advantages and limitations.

Findings
Good correlation with the destructive method has been obtained. The proposed method enables more detailed analysis of a joint as arbitrarily oriented cross-section for analysed area can be easily generated and increase of measurements number is always possible and simple. The disadvantage of the method is lower accuracy of diameter determination than in the case of conventional methods.

Research limitations/implications
The measurements were performed only on one type of specimens. Probably, if a rivet and sheets were made of the same alloy, the measurements would be barely possible. The rivets were installed with squeezing ratio D/Do = 1.7 whose value is close to maximum as defined in riveting instructions (Kaniowski, 2015). This means that measured hole expansions were higher than in typical joint. The proposed method is appropriate for simple specimens (one rivet at a specimen width).

Practical implications
The investigation shows that rivet hole expansion can be measured with the use of CT. This method is useful especially when destruction of a specimen is not allowed or more detailed analysis is required (e.g. measurements on many depth levels).

Originality/value
The paper presents measurements of rivet hole expansion with the method which has not been used before for this application. Advantages and limitations of the proposed approach compared to conventional methods are discussed.
Flow-separation-control system operating in feedback closed loop
Authors: Stalewski Wienczyslaw , Krzysiak Andrzej 
Published: October 2018

Purpose
The purpose of this study is to develop the concept of self-adapting system which would be able to control a flow on the wing-high-lift system and protect the flow against strong separation.

Design/methodology/approach
The self-adapting system has been developed based on computational approach. The computational studies have been conducted using the URANS solver. The experimental investigations have been conducted to verify the computational results.

Findings
The developed solution is controlled by closed-loop-control (CLC) system. As flow actuators, the main-wing trailing-edge nozzles are proposed. Based on signals received from the pressure sensors located at the flap trailing edge, the CLC algorithm changes the amount of air blown from the nozzles. The results of computational simulations confirmed good effectiveness and reliability of the developed system. These results have been partially confirmed by experimental investigations.

Research limitations/implications
The presented research on an improvement of the effectiveness of high-lift systems of modern aircraft was conducted on the relatively lower level of the technology readiness. However, despite this limitation, the results of presented studies can provide a basis for developing innovative self-adaptive aerodynamic systems that potentially may be implemented in future aircrafts.

Practical implications
The studies on autonomous flow-separation control systems, operating in a closed feedback loop, are a great hope for significant advances in modern aeronautical engineering, also in the UAV area. The results of the presented studies can provide a basis for developing innovative self-adaptive aerodynamic systems at a higher level of technological readiness.

Originality/value
The presented approach is especially original and valuable in relation to the innovative concept of high-lift system supported by air-jets blown form the main-wing-trailing-edge nozzles; the effective and reliable flow sensors are the pressure sensors located at the flap trailing edge, and the effective and robust algorithm controlling the self-adapting aerodynamic system – original especially in respect to a strategy of deactivation of flow actuators.
A (2 + 1)-dimensional extension of the Benjamin-Ono equation
Authors: Wazwaz Abdul-Majid 
Published: October 2018

Purpose
The purpose of this paper is concerned with developing a (2 + 1)-dimensional Benjamin–Ono equation. The study shows that multiple soliton solutions exist and multiple complex soliton solutions exist for this equation.

Design/methodology/approach
The proposed model has been handled by using the Hirota’s method. Other techniques were used to obtain traveling wave solutions.

Findings
The examined extension of the Benjamin–Ono model features interesting results in propagation of waves and fluid flow.

Research limitations/implications
The paper presents a new efficient algorithm for constructing extended models which give a variety of multiple soliton solutions.

Practical implications
This work is entirely new and provides new findings, where although the new model gives multiple soliton solutions, it is nonintegrable.

Originality/value
The work develops two complete sets of multiple soliton solutions, the first set is real solitons, whereas the second set is complex solitons.
Unsteady MHD mixed convection along an exponentially stretching surface
Authors: Patil Prabhugouda Mallanagouda , A. Shashikant , Momoniat Ebrahim 
Published: October 2018

Purpose
This paper aims to investigate the unsteady mixed convection along an exponentially stretching surface in presence of transverse magnetic field applied at the wall and the opposing buoyancy flow.

Design/methodology/approach
The dimensional partial differential equations governing the flow field are transformed to non-dimensional coupled partial differential equations with the aid of suitable non-similar transformations. The resulting equations are then solved by the coalition of quasilinearization technique and the finite difference method.

Findings
Effects of volumetric heat source/sink, suction/blowing and other dimensionless parameters on velocity and temperature profiles are examined numerically. This investigation reveals that in presence of opposing buoyancy flow, the suction and volumetric heat source enhances the skin-friction coefficient, while the rise in the MHD increases the momentum boundary layer.

Originality/value
To the best of the authors’ knowledge, no such investigation has been carried out in the literature.
Linking marketing mix elements to passion-driven behavior toward a brand
Authors: Kim Min-Seong , Kim Jihye 
Published: October 2018

Purpose
This study aims to investigate the relationships among perceptions of advertising, sales promotions, brand prestige and brand love, as well as the development of passion-driven behavior among family restaurant customers. Based on the well-established framework of marketing mix elements and brand, this study proposes and tests a research model that attempts to understand the inter-relationships between the two dimensions of advertising (i.e. advertising spending and attitudes toward advertisement), two dimensions of sales promotions (i.e. monetary promotion and non-monetary promotion), brand prestige, brand love and passion-driven behavior, as well as compares local and global family restaurant brands.

Design/methodology/approach
The research model of this study was tested based on responses from 265 family restaurant customers in Korea (i.e. 105 from Outback Steak House, 78 from T.G.I. Fridays, 45 from VIPS and 37 from Ashley). Data were analyzed using frequency, reliability, confirmatory factor and correlation analyses, the structural equation modeling and multi-sample methods.

Findings
The verified model confirmed that brand prestige was significantly influenced by attitudes toward advertisement, monetary promotion and non-monetary promotion. Also, brand love was significantly affected by the amount spent on advertising, attitudes toward the advertisement and monetary promotion. Passion-driven behavior was significantly impacted by brand prestige and brand love. Lastly, the results identified that the brand origin (i.e. local and global family restaurant brands) moderated one of the hypothesized relationships (i.e. the impact of brand prestige on brand love).

Practical implications
The study suggests that a family restaurant brand manager may want to emphasize planning and developing marketing mix elements focusing on advertising and sales promotions.

Originality/value
This research illustrates the influences of the two dimensions of advertising and two types of sales promotions on brand prestige and brand love, which in turn led to passion-driven behavior from family restaurant customers.
Investigation on spatial distribution and evolution features of the running-in attractor based on a new approach
Authors: Ding Cong , Zhu Hua , Sun Guodong , Wei Chun Ling , Jiang Yu 
Published: October 2018

Purpose
The purpose of this work is to comprehensively reveal the spatial distribution and evolution features of a running-in attractor.

Design/methodology/approach
The friction coefficient signals extracted from wear experiments are reconstructed. A projected matrix is obtained based on the reconstructed matrix. Then the approach of three-dimensional (3D) histogram of phase points is proposed, which is used to intuitively characterize the complex properties of the running-in attractor.

Findings
The space occupied by the running-in attractor gradually contracts, then stabilizes and finally expands; the maximum of phase points number in a certain bin initially decreases, then keeps stable and finally increases rapidly; yet the percentage of bins number storing phase points shows an inverse variation tendency. Consequently, 3D histogram evolves from a nonuniform state to a uniform state then returns back to the nonuniform state, which indicates the evolution rule of “formation, stabilization and disappearance” of the running-in attractor.

Originality/value
Characterization on the features of the running-in attractor can provide valuable information about friction systems and their dynamic behaviors.
Design for safety implementation factors: a literature review
Authors: Poghosyan Anush , Manu Patrick , Mahdjoubi Lamine , Gibb Alistair G. F. , Behm Michael , Mahamadu Abdul-Majeed 
Published: October 2018

Purpose
Decisions made during the design stage of construction works can significantly reduce the risk of occurrence of occupational accidents, injuries and illnesses. Moreover, it has been established that design is one of the major contributors of accidents and injuries. Design for safety (DfS) studies within construction have highlighted factors affecting the implementation of DfS, among which are designer attitude; DfS knowledge/awareness and education; availability of DfS tools, including guidance; client’s influence and motivation; and legislation. The purpose of this study is to carry out an in-depth literature review of DfS studies within construction to explore the extent to which existing DfS research has looked at the above-listed DfS implementation factors.

Design/methodology/approach
A review of 164 journal articles related to DfS in construction (published from 1990 to 2017) within built environment, engineering and multidisciplinary safety journals was undertaken.

Findings
The findings indicate that around 60 per cent of the journal articles reviewed address designer knowledge/awareness and education issues, about 27 per cent looked at DfS implementation tools to assist designers to undertake DfS, about 23 per cent studied client influence/motivation, about 16 per cent studied designers’ attitudes towards DfS implementation and approximately 16 per cent looked at the role of legislation in DfS implementation. The literature points that client influence/motivation and legislation are very influential DfS implementation factors despite a limited number of studies in these areas.

Originality/value
Overall, the findings provide an indication of areas of DfS implementation, particularly client influence/motivation and legislation, where more research would be needed to promote DfS in construction to help mitigate the occurrence of accidents and injuries.
Price risk management in BOT railroad construction projects using financial derivatives
Authors: Vahdatmanesh Mohammad , Firouzi Afshin 
Published: October 2018

PurposeRailroad transit infrastructures are amongst major capital-intensive projects worldwide, which impose significant risks to the contractors of build-operate-transfer projects because of the fluctuations in steel price fluctuation. The purpose of this paper is to introduce a methodology for hedging steel price risk using financial derivatives.Design/methodology/approachCox–Ross valuation lattice has been used as an option valuation model for determining option’s price for the construction companies involved in fixed-price railroad projects. A sensitivity analysis has been conducted using the financial option Greeks to evaluate the impacts of option’s pricing factors in the total price of option.FindingsThe result of valuation shows that European options cost to safeguard against the effects of price risk is only a fraction in contrast to the total cost of steel procurement for a typical railroad construction company. This confirms that using this kind of financial derivative is a beneficial yet effective approach for hedging steel price risk for railroad construction companies.Practical implicationsThe applicability of the financial derivatives, both exchange-traded and over-the-counter instruments, is evident in broad financial industry. This paper shows how European options can be readily used for risk management of a typical railroad project, and explains the methodology in a step-by-step procedure.Originality/valueAlthough the financial engineering literature is rife of theory and application of derivatives in various contexts, to the best knowledge of authors there is only few papers on the application of these well-developed financial instruments for risk management in construction industry. This study intends to illustrate how financial derivatives can add value to risky construction projects and shed new light in this important application area.