bluuebunny/crossref_metadata_2025_split huggingface dataset:
Novel manufacturing method for producing apohemoglobin and its biophysical properties
Authors: Pires Ivan S. , Belcher Donald A. , Hickey Richard , Miller Colbert , Badu‐Tawiah Abraham K. , Baek Jin Hyen , Buehler Paul W. , Palmer Andre F. 
Published: October 2019

AbstractApohemoglobin (apoHb) is a dimeric globular protein with two vacant heme‐binding pockets that can bind heme or other hydrophobic ligands. Purification of apoHb is based on partial hemoglobin (Hb) unfolding to facilitate heme extraction into an organic solvent. However, current production methods are time consuming, difficult to scale up, and use highly flammable and toxic solvents. In this study, a novel and scalable apoHb production method was developed using an acidified ethanol solution to extract the hydrophobic heme ligand into solution and tangential flow filtration to separate heme from the resultant apoprotein. Total protein and active protein yields were >95% and ~75%, respectively, with <1% residual heme in apoHb preparations and >99% purity from sodium dodecyl sulfate–polyacrylamide gel electrophoresis analysis. Virtually no loss of apoHb activity was detected at 4°C, −80°C, and in lyophilized form during long term storage. Structurally, size exclusion chromatography (SEC) and circular dichroism indicated that apoHb was dimeric with a ~25% reduction of helical content compared to Hb. Furthermore, mass spectroscopy and reverse‐phase chromatography indicated that the mass of the α and β subunits were virtually identical to the theoretical mass of these subunits in Hb and had no detectable oxidative modifications upon heme removal from Hb. SEC confirmed that apoHb bound to haptoglobin at a similar ratio to that of native Hb. Finally, reconstituted Hb (rHb) was processed via a hemichrome removal method to isolate functional rHb for biophysical characterization in which the O2equilibrium curve, O2dissociation, and CO association kinetics of rHb were virtually identical to native Hb. Overall, this study describes a novel and improved method to produce apoHb, as well as presents a comprehensive biochemical analysis of apoHb and rHb.
Evaluation of the psychoemotional status of young adults with symptoms of temporomandibular disorders
Authors: Maślak‐Bereś Monika , Loster Jolanta E. , Wieczorek Aneta , Loster Bartłomiej W. 
Published: October 2019

AbstractBackground and purposeTemporomandibular disorders (TMD) are among the most frequent pathologies of the stomatognathic system. One problem often associated with TMD is the psychoemotional status. The aim of study was to evaluate the psychoemotional status of young adults with pain symptoms associated with TMD.Material and methodsWe analyzed the data of 260 volunteers. The Research Diagnostic Criteria for Temporomandibular Disorders (RDC/TMD) form was used to diagnose TMD. The relationships between TMD/RDC clinical diagnoses and psychoemotional status, as described by the Beck's Depression Inventory (BDI) and Perceived Stress Scale (PSS‐10), were analyzed. We divide the group into four on the basis of RDC/TMD Axis I diagnosis. Group 0 included 30 students lacking TMD symptoms. Group I consisted of 30 people with myofascial pain (group IA in RDC/TMD). Group II contained 23 people with disk displacement with reduction (group IIA in RDC/TMD). Group III contained ten people (Group III diagnosis, often associated with pain).ResultsWe did not find statistically significant differences between the study groups. In subjects with pain (Groups I and III), we found the mean value on the BDI and PSS‐10 scales to be higher than among the pain‐free subjects (Groups 0 and II).ConclusionIn young adults with TMD accompanied by pain, psychoemotional status should also be evaluated.
Single‐Flap Approach in Periodontal Regeneration for Intraosseous Defects: Case Series
Authors: Oh Se‐Lim , Joshi Shashank 
Published: October 2019

IntroductionA single‐flap approach (SFA) is the elevation of a periodontal flap to access the defect only from one side. Several studies have reported that the SFA is at least as clinically effective as the elevation of a flap at both buccal and palatal/lingual aspects. However, studies regarding the SFA have reported only 6 to 10 months follow‐up clinical outcomes. The purpose of this case series was to investigate the outcomes of the SFA for periodontal regeneration with a collagen membrane and bone grafts in regard to linear bone defect fill and clinical parameters such as gingival recession (GR), pocket depth (PD), and clinical attachment level (CAL) for the 15‐month follow‐up.Case SeriesBased on the deepest pocket depth site, the flap retraction side for each case, either buccal or palatal/lingual, was determined. After retraction of a one‐side full thickness flap, complete removal of granulation tissue and thorough scaling and root planing were performed. Demineralized ground cortical bone grafts were gently packed into the defect areas and an absorbable collagen membrane was placed over the grafts in 13 cases from 11 patients.ConclusionThis case series demonstrated that the SFA for periodontal regeneration with a collagen membrane and bone grafts resulted in decreased PD with minimum GR, gain in CAL, and bone fill at defect sites for the 15‐month follow‐up.
Novel Metabolites from the Endophytic Fungus Chaetomium subaffine L01
Authors: Han Xiao‐Yang , Xie Yi‐Xuan , Wu Chao‐Qun , Ai Hong‐Lian , Lei Xin‐Xiang , Wang Xiang‐Jing 
Published: October 2019

AbstractOne natural p‐terphenyl glycoside, gliocladinin C, and two furano‐polyene derivatives, chaetominins A and B, were isolated from potato endophytic fungus Chaetomium subaffine. The absolute configurations of these compounds were elucidated by HR‐ESI‐MS, NMR, the DP4+ probabilities and electronic circular dichroism (ECD) spectra. Furthermore, gliocladinin C and chaetominin A showed cytotoxic activity against two selected human tumor cell lines (Hep‐2 and HepG‐2).
The Cul4‐DDB1‐WDR3/WDR6 Complex Binds SPAK and OSR1 Kinases in a Phosphorylation‐Dependent Manner
Authors: Dhiani Binar A. , Mehellou Youcef 
Published: October 2019

AbstractSPAK and OSR1 are two protein kinases that play critical roles in regulating ion homeostasis. They are activated under osmotic stress through phosphorylation by their upstream WNK kinases at a conserved threonine site on their T‐loops. Additionally, WNK kinases phosphorylate SPAK and OSR1 at a highly conserved serine residue on their S‐motif, the function of which remains elusive. Using affinity pull down and mass spectrometry, we identified the E3 ubiquitin ligase complex Cullin 4‐DDB1‐WDR3/WDR6 as a binder to OSR1 kinase in a SPAK/OSR1 S‐motif phosphorylation‐dependent manner. This binding was found to be compromised by S‐motif phosphorylation following osmotic stress. Using proteasomal and neddylation inhibitors, we subsequently showed that OSR1 ubiquitylation was abolished under osmotic stress when its S‐motif is phosphorylated. These results provide the first example of an E3 ubiquitin ligase system that binds the OSR1 kinase and, thus, links the CRL4 complex to ion homeostasis.
Analysis of the Substrate Specificity of the SMYD2 Protein Lysine Methyltransferase and Discovery of Novel Non‐Histone Substrates
Authors: Weirich Sara , Schuhmacher Maren Kirstin , Kudithipudi Srikanth , Lungu Cristiana , Ferguson Andrew D. , Jeltsch Albert 
Published: October 2019

AbstractThe SMYD2 protein lysine methyltransferase methylates various histone and non‐histone proteins and is overexpressed in several cancers. Using peptide arrays, we investigated the substrate specificity of the enzyme, revealing a recognition of leucine (or weaker phenylalanine) at the −1 peptide site and disfavor of acidic residues at the +1 to +3 sites. Using this motif, novel SMYD2 peptide substrates were identified, leading to the discovery of 32 novel peptide substrates with a validated target site. Among them, 19 were previously reported to be methylated at the target lysine in human cells, strongly suggesting that SMYD2 is the protein lysine methyltransferase responsible for this activity. Methylation of some of the novel peptide substrates was tested at the protein level, leading to the identification of 14 novel protein substrates of SMYD2, six of which were more strongly methylated than p53, the best SMYD2 substrate described so far. The novel SMYD2 substrate proteins are involved in diverse biological processes such as chromatin regulation, transcription, and intracellular signaling. The results of our study provide a fundament for future investigations into the role of this important enzyme in normal development and cancer.
Upstream anticoagulation for patients with ST‐elevation myocardial infarction undergoing primary percutaneous coronary intervention: Insights from the TOTAL trial
Authors: Cantor Warren J. , Lavi Shahar , Džavík Vladimír , Cairns John , Cheema Asim N. , Della Siega Anthony , Moreno Raul , Stankovic Goran , Kedev Sasko , Natarajan Madhu K. , Levi Yaniv , Yuan Fei , Jolly Sanjit S. 
Published: October 2019

AbstractObjectivesTo assess the relationship between preprocedural anticoagulation use and clinical and angiographic outcomes.BackgroundFor patients with ST‐elevation myocardial infarction (STEMI) undergoing primary percutaneous coronary intervention (PCI), the optimal timing of anticoagulant administration remains uncertain.MethodsPatients enrolled in the TOTAL trial were stratified based on whether or not they had received any parenteral anticoagulant prior to randomization and PCI. Baseline and procedural characteristics were compared. For one‐year clinical outcomes, Cox proportional modeling adjusted on a propensity score was used to analyze differences between groups. Angiographic endpoints were analyzed by logistic regression models adjusted for propensity scores.ResultsIn the trial, 10,064 patients were enrolled and underwent PCI. Preprocedural anticoagulation was used in 6,381 patients (63%).The most common anticoagulant was intravenous unfractionated heparin (5,188, 81%). Patients who received preprocedural anticoagulation had higher rates of TIMI‐2‐3 or TIMI‐3 flow and lower grades of thrombus prior to PCI. Pretreatment with anticoagulation was associated with lower use of bailout thrombectomy, GP IIb/IIIa inhibitors, and intra‐aortic balloon pump. After adjustment, preprocedural anticoagulation was associated with lower rates of CABG and minor bleeding at 1 year but there were no significant differences in death, stroke, recurrent MI, cardiogenic shock, or congestive heart failure.ConclusionsPreprocedural anticoagulation is associated with improved flow and reduced thrombus in the IRA prior to PCI, less bailout thrombectomy during PCI but no difference in death, recurrent infarction, or heart failure at 1 year.
Vibrational Quenching in Near‐Infrared Emitting Lanthanide Complexes: A Quantitative Experimental Study and Novel Insights
Authors: Mara Dimitrije , Artizzu Flavia , Smet Philippe F. , Kaczmarek Anna M. , Van Hecke Kristof , Van Deun Rik 
Published: October 2019

AbstractTwo series of novel NIR‐emissive complexes of Nd3+, Sm3+, Er3+ and Yb3+ with two different β‐diketonate ligands (L1=4,4,4‐trifluoro‐1‐phenyl‐1,3‐butadione and L2=4,4,4‐trifluoro‐1‐(4‐chlorophenyl)‐1,3‐butadione) are reported. The neutral triphenylphosphine oxide (tppo) ligand was used to replace coordinated water molecules in the first coordination sphere of the as‐obtained [Ln(L1(2))3(H2O)2] complexes to afford water‐free [Ln(L1(2))3(tppo)2] molecular species. Upon replacement of water molecules by tppo units, the NIR emission lifetimes of the Nd3+, Er3+and Sm3+complexes increase by about one order of magnitude up to values of ≈9, 8 and 113 ms while Yb3+ complexes reach intrinsic quantum yields as high as to ΦYb=6.5 %., which are remarkably high for fully hydrogenated complexes. Vibrational quenching by CH and OH oscillators has been quantitatively assessed by implementing the Förster's model of resonance energy transfer on the basis of experimental data. This study demonstrates that highly efficient NIR‐emitting lanthanide complexes can be obtained with facile, cheap and accessible syntheses through a rational design.
Crystal Growth of Materials with the ThCr2Si2 Structure Type
Authors: Kliemt Kristin , Peters Marius , Feldmann Fabian , Kraiker Alexej , Tran Doan‐My , Rongstock Susanna , Hellwig Johannes , Witt Sebastian , Bolte Michael , Krellner Cornelius 
Published: October 2019

AbstractThe single crystal growth of 19 different intermetallic compounds within the LnT2X2 family (with Ln = lanthanides, T = Co, Ru, Rh, Ir, and X = Si, P) is presented, by employing a high‐temperature metal‐flux technique. The habitus of the obtained crystals is platelet‐like with the crystallographic c direction perpendicular to the surface and with individual masses between 1 and 100 mg. The magnetic properties of these crystals are characterized by magnetization, heat‐capacity, and resistivity measurements. These crystals form the materials basis for a thorough study of exciting surface properties by angle‐resolved photoemission spectroscopy.
Biomass‐based Hierarchical Porous Carbon for Supercapacitors: Effect of Aqueous and Organic Electrolytes on the Electrochemical Performance
Authors: Chen Zhimin , Wang Xiaofeng , Ding Zhiyao , Wei Qingling , Wang Zichen , Yang Xiaomin , Qiu Jieshan 
Published: October 2019

AbstractBiomass‐based hierarchical porous carbon (SCPC) exhibits excellent electrochemical performance in electric double layer capacitors, prepared by carbonization and activation of straw cellulose. To investigate the potential applications of SCPC in supercapacitors, the effect of aqueous and organic electrolytes on the electrochemical performance of SCPC was studied in detail. In H2SO4, the SCPC electrode exhibits higher specific capacitance (358 F g−1) and outstanding cycling stability with 95.6 % capacitance retention over 10 000 cycles. The SCPC electrode shows superior rate capability with 90.7 % capacitance retention in KOH, and higher energy density of 17.9 Wh kg−1 in Na2SO4. The SCPC electrode exhibits ideal capacitance characteristics, superior rate capability with capacitance retention of 95.8 %, and high energy density of 36.0 Wh kg−1 in tetraethylammonium tetrafluoroborate/propylene carbonate (Et4NBF4/PC). The significant difference of capacitive performance of SCPC electrode in various electrolytes is mainly attributed to the difference in the electrolyte ion size, ionic conductivity, matching between the electrolyte ions and pore structure, and matching between anions and cations adsorbed on the positive and negative electrodes. This work not only establishes the relationship between the structure of SCPC and its electrochemical performance in different electrolytes, but also provides a reference for the high value‐added utilization of SCPC.
Metal–Organic Framework‐Derived Fe/Co‐based Bifunctional Electrode for H2 Production through Water and Urea Electrolysis
Authors: Singh Thangjam Ibomcha , Rajeshkhanna Gaddam , Singh Soram Bobby , Kshetri Tolendra , Kim Nam Hoon , Lee Joong Hee 
Published: October 2019

AbstractHollow‐structured FexCo2−xP, FexCo3−xO4, and Prussian blue analogue (FeCo‐PBA) microbuilding arrays on Ni foam (NF) are derived from Co‐based metal–organic frameworks (Co‐MOF) using a simple room temperature and post‐heat‐treatment route. Among them, FexCo2−xP/NF shows excellent bifunctional catalytic activities by demonstrating very low oxygen evolution reaction (OER) and hydrogen evolution reaction (HER) overpotentials of 255/114 mV at a current density of 20/10 mA cm−2 respectively, whereas FexCo3−xO4/NF and FeCo‐PBA/NF demand higher overpotentials. Remarkably, for water electrolysis, FexCo2−xP/NF requires only 1.61 V to obtain 10 mA cm−2. In contrast to water electrolysis, urea electrolysis reduces overpotential and simultaneously purifies the urea‐rich wastewater. The urea oxidation reaction at the FexCo2−xP/NF anode needs just 1.345 V to achieve 20 mA cm−2, which is 140 mV less than the 1.48 V potential required for OER. Moreover, the generation of H2 through urea electrolysis needs only 1.42 V to drive 10 mA cm−2.
Flexible Freestanding MoO3−x–Carbon Nanotubes–Nanocellulose Paper Electrodes for Charge‐Storage Applications
Authors: Etman Ahmed S. , Wang Zhaohui , El Ghazaly Ahmed , Sun Junliang , Nyholm Leif , Rosen Johanna 
Published: October 2019

AbstractHerein, a one‐step synthesis protocol was developed for synthesizing freestanding/flexible paper electrodes composed of nanostructured molybdenum oxide (MoO3−x) embedded in a carbon nanotube (CNT) and Cladophora cellulose (CC) matrix. The preparation method involved sonication of the precursors, nanostructured MoO3−x, CNTs, and CC with weight ratios of 7:2:1, in a water/ethanol mixture, followed by vacuum filtration. The electrodes were straightforward to handle and possessed a thickness of approximately 12 μm and a mass loading of MoO3−x–CNTs of approximately 0.9 mg cm−2. The elemental mapping showed that the nanostructured MoO3−x was uniformly embedded inside the CNTs–CC matrix. The MoO3−x–CNTs–CC paper electrodes featured a capacity of 30 C g−1, normalized to the mass of MoO3−x–CNTs, at a current density of 78 A g−1 (corresponding to a rate of approximately 210 C based on the MoO3 content, assuming a theoretical capacity of 1339 C g−1), and exhibited a capacity retention of 91 % over 30 000 cycles. This study paves the way for the manufacturing of flexible/freestanding nanostructured MoO3−x‐based electrodes for use in charge‐storage devices at high charge/discharge rates.
Comparison of inhibition resistance among PCR reagents for detection and quantification of environmental DNA
Authors: Uchii Kimiko , Doi Hideyuki , Okahashi Teruyuki , Katano Izumi , Yamanaka Hiroki , Sakata Masayuki K. , Minamoto Toshifumi 
Published: October 2019

AbstractBackgroundReal‐time PCR has been widely employed in environmental DNA (eDNA) surveys to detect and quantify target species. The biggest obstacle in real‐time PCR based eDNA assays is the inhibition of PCR amplification that results in false‐negative detection and uncertainty of quantification.AimsHere, we aimed to evaluate inhibition resistance of PCR reagents for detection and quantification of environmental DNA.Materials & MethodsWe compared six commercial PCR reagents, including four inhibitor‐resistant reagents, for resistance to major PCR inhibitory substances, i.e., humic, fulvic, and tannic acids. Then, we selected three inhibitorresistant reagents to test for their resistance to multiple natural inhibitors using field eDNA samples collected from various habitats.ResultsWe found that each inhibitor‐resistant reagent had its advantage and disadvantage depending on which inhibitory substance was used. Similarly, the inhibitory effects caused by field samples differed among the three inhibitor‐resistant reagents, where none of the reagents consistently showed strong resistance to all field samples.ConclusionOur results indicate that the selection of appropriate PCR reagent would greatly ease PCR inhibition, and consequently improve the estimation of species distribution by real‐time PCR‐based eDNA assays.
Trajectories of multisite musculoskeletal pain in midlife: Associations with common mental disorders
Authors: Neupane Subas , Lallukka Tea , Pietiläinen Olli , Rahkonen Ossi , Leino‐Arjas Päivi 
Published: October 2019

AbstractObjectivesWe examined developmental trajectories of multisite musculoskeletal pain in midlife, and their associations with mental well‐being.MethodsMidlife municipal employees at baseline aged 40, 45, 50, 55 or 60 years (80% women) from the City of Helsinki, Finland, responded to a baseline questionnaire in 2000–02 (N = 8,960; response rate 67%) and follow‐ups in 2007 (N = 7,332; 83%) and 2012 (N = 6,809; 78%). Trajectories of the number of pain sites (0–4) were modelled using latent class growth analysis (n = 6,527). Common mental disorders were assessed by the General Health Questionnaire (GHQ) 12‐item version (trichotomized to low, intermediate or high). Information on health‐related behaviour, comorbidity and socioeconomic position was obtained from the questionnaire. Associations of baseline factors with pain trajectories were assessed by multinomial logistic regression.ResultsWe identified four distinct pain trajectories: high (15%), increasing (24%), decreasing (20%) and low (41%). After an initial increase, the high and increasing trajectories stabilized at around 2.5 and 1.5 pain sites respectively. In a multivariable model, high, increasing and decreasing trajectories of pain sites were associated with higher baseline GHQ scores. The association was strongest for the high trajectory (low GHQ: OR 3.7, 95% CI 2.8–4.9; high GHQ: OR 5.4, 95% 4.4–6.6). Trajectory membership also associated with unhealthy behaviours, musculoskeletal comorbidities and a low socioeconomic position at baseline. Average GHQ was consistently highest for the high pain trajectory and decreased in the decreasing trajectory over the follow‐up.ConclusionsMultisite musculoskeletal pain shows variable developmental patterns among midlife employees. The trajectories are associated with the level of common mental disorders.SignificanceFour developmental trajectories of multisite pain in midlife were described over 10–12 years of follow‐up: low (41% of the sample), increasing (24%), high (15%) and decreasing (20%). Common mental disorders strongly associated with these. Belonging to the highest tertile of mental disorders at baseline increased the risk of membership in the high trajectory more than fivefold. On the other hand, together with a decrease in mental disorders, the number of pain sites decreased to zero.
A Novel Chronoimpedimetric Glucose Sensor in Real Blood Samples Modified by Glucose‐imprinted Pyrrole‐Aminophenylboronic Acid Modified Screen Printed Electrode
Authors: Onur Uygun Zihni , Ertuğrul Uygun Hilmiye Deniz 
Published: October 2019

AbstractIn this study, a molecularly imprinted sensor technology is engineered to detect glucose in real blood samples by chronoimpedimetrically. The imprinting process of glucose (Glc) was carried out by electrochemical polymerization of aminophenylboronic acid (APBA) and pyrrole (Py) by performing cyclic voltammetry (CV). Afterwards, glucose molecule was removed from imprinted surface by 5 % acetic acid to reveal glucose imprinted cavities. Electrochemical Impedance Spectroscopy (EIS) was used to characterize sensor modification steps and glucose removal. Glucose monitoring process was carried out chronoimpedimetrically(CI) for the first time in real blood samples. Calibration curve was prepared between 20–800 mg/dL. The standard deviations of the 18 calibration curves R2 were calculated as 0.9866±0.0066 to assess reproducibility. Recovery was calculated by using 105 mg/dL Glc Serum Sample, which was monitored by auto analyzer and into this sample 50 mg/dL Glc added and our sensor response was 147.92±2.43 mg/dL, 98.6±1.62 % (n=5). Non‐imprinted (NIP) sensor gave no signal for the glucose concentration.