bluuebunny/crossref_metadata_2025_split huggingface dataset:
microRNA‐206 overexpression inhibits epithelial‐mesenchymal transition and glomerulosclerosis in rats with chronic kidney disease by inhibiting JAK/STAT signaling pathway
Authors: Zhao Shi‐Qi , Shen Zhao‐Chun , Gao Bing‐Feng , Han Ping 
Published: May 2019

AbstractChronic kidney disease (CKD) is a traumatic disease with significant psychic consequences to the patient's overall physical condition. microRNA‐206 (miR‐206) has been reported to play an essential role in the development of various diseases. The purpose of the present study is to investigate the effect of miR‐206 through the JAK/STAT signaling pathway on epithelial‐mesenchymal transition (EMT) of renal tubular epithelial cells and glomerulosclerosis in rats with CKD. The targeting relationship between miR‐206 and ANXA1 was verified. To explore the role of miR‐206 in CKD, the model of CKD rats was established to detect glomerular sclerosis index (GSI), contents of interleukin‐6 (IL‐6) and transforming growth factor‐beta1 (TGF‐β1), and expression of type IV collagen. Moreover, to further determine the roles of both miR‐206 and the JAK/STAT signaling pathway in CKD, the gain‐ and loss‐of function approaches were performed with the expression of ANXA1, α‐SMA, E‐cadherin, vimentin, N‐cadherin, and the JAK/STAT signaling pathway‐related genes detected. miR‐206 negatively targeted ANXA1. Overexpressed miR‐206 inhibited the degeneration and interstitial fibrosis of renal tubular epithelial cells, decreased GSI of rats, and the expression of type IV collagen, TGF‐β1 and IL‐6. Overexpressed miR‐206 inhibited the degeneration of renal tubular epithelial cells, the expression of ANXA1, α‐SMA, TGF‐β1, p‐STAT3, STAT3, p‐STAT1, STAT1, p‐JAK2, and JAK2, while promoted the expression of E‐cadherin. Taken together the results, miR‐206 inhibits EMT of renal tubular epithelial cells and glomerulosclerosis by inactivating the JAK/STAT signaling pathway via ANXA1 in CKD.
Circular RNA circ_0079593 indicates a poor prognosis and facilitates cell growth and invasion by sponging miR‐182 and miR‐433 in glioma
Authors: Qu Yi , Zhu Jian , Liu Jianyong , Qi Li 
Published: May 2019

AbstractGlioma is one of the major global health problems, including in China. Circular RNAs (circRNAs) have been increasingly identified and characterized in almost every aspect of biology, especially in cancer biology. This research desires to explore the functions and mechanism of a novel circRNA, circ_0079593, on regulating glioma progression. Quantitative real‐time polymerase chain reaction (qRT‐PCR) was carried out to measure the relative expression of circ_0079593, which was upregulated in matched cancerous tissues from 60 patients and four cell lines of glioma. A higher level of circ_0079593 in glioma specimens was linked to larger tumor size, higher WHO grade, and worse survival rate for patients with glioma. Moreover, circ_0079593 can be deemed as an independent prognostic predictor for glioma patients analyzed by multivariate method. Cell counting kit‐8, flow cytometric, wound healing, and transwell experiments were used to evaluate cell growth, apoptosis, migration, and invasion influenced by circ_0079593 knockdown/overexpression. Exogenous downregulation of circ_0079593 expression significantly suppressed glioma cell proliferation by increasing cell apoptosis in vitro, and retarded the migratory and invasive potential. Ectopic expressed circ_0079593 could induce the opposite effects. Mechanistically, bioinformatics analysis, qRT‐PCR, and dual‐luciferase reporter assays showed that microRNA 182 (miR‐182) and miR‐433 could be sponged and negatively regulated by circ_0079593. Further, rescue assays demonstrated that the biological functions of circ_0079593 are dependent on its inhibition of miR‐182 and miR‐433. Collectively, the present work indicates that circ_0079593 may be used as an effective prognostic marker and therapeutic target for glioma.
Hepatic symptoms and histology in 13 patients with a Zellweger spectrum disorder
Authors: Berendse Kevin , Koot Bart G. P. , Klouwer Femke C. C. , Engelen Marc , Roels Frank , Lacle Miangela M. , Nikkels Peter G. J. , Verheij Joanne , Poll‐The Bwee Tien 
Published: May 2019

AbstractPatients with a Zellweger spectrum disorder (ZSD) have a defect in the assembly or maintenance of peroxisomes, leading to a multisystem disease with variable outcome. Liver disease is an important feature in patients with severe and milder phenotypes and a frequent cause of death. However, the course and histology of liver disease in ZSD patients are ill‐defined. We reviewed the hepatic symptoms and histological findings of 13 patients with a ZSD in which one or several liver biopsies have been performed (patient age 0.2‐39 years). All patients had at least some histological liver abnormalities, ranging from minor fibrosis to cirrhosis. Five patients demonstrated significant disease progression with liver failure and early death. In others, liver‐related symptoms were absent, although some still silently developed cirrhosis. Patients with peroxisomal mosaicism had a better prognosis. In addition, we show that patients are at risk to develop a hepatocellular carcinoma (HCC), as one patient developed a HCC at the age of 36 years and one patient a precancerous lesion at the age of 18 years. Thus, regular examination to detect fibrosis or cirrhosis should be included in the standard care of ZSD patients. In case of advanced fibrosis/cirrhosis expert consultation and HCC screening should be initiated. This study further delineates the spectrum and significance of liver involvement in ZSDs.
Predictability of East Asian summer monsoon in seasonal climate forecast models
Authors: Liu Yunyun , Ke Zongjian , Ding Yihui 
Published: May 2019

AbstractThe prediction skill and source of the predictability of the East Asian summer monsoon (EASM) system are examined in this work based on four state‐of‐the‐art seasonal climate forecast models including BCC_CSM1.1, ECMWF_SYS4, NCEP_CFS2 and TCC_CPS2. The prediction of the climatology and interannual EASM pattern and the impact on the prediction are further investigated. It is noted that the four models have some skill in predicting summer rainfall in the East Asia, however, the skill is low on average and also largely regional dependence. The interannual variation of EASM measured by monsoon circulation index is well reproduced, implying that the broad‐scale feature/pattern of EASM has higher predictability than the detailed spatial variation of EASM rainfall. The possible sources of predictability of the interannual variability of EASM are associated with the El Niño‐Southern Oscillation (ENSO) and the north Indian Ocean (NIO) sea surface temperature (SST) anomalies. The correlation pattern of rainfall with the NIO SST is characterized by a tripole pattern from south to north of East Asia, which is different from the correlation distribution of the southern‐northern dipole with ENSO, suggesting that NIO SST may exert influence on the EASM independently. The major biases in climatology of EASM in the models are the northward shift of the western Pacific subtropical high (WPSH) and weak monsoonal southerly over the coast of East Asia, which leads to the prediction bias of the Meiyu/Baiu/Changma (MBC) rainfall belt. The prediction of the interannual EASM pattern presents two deficiencies: too weak rainfall variability and northward shift of the dipole rainfall pattern (opposite variation between MBC and the northwestern Pacific), that may be caused by the biases of WPSH in the models.
Gelation Process and Physicochemical Properties of Thai Silk Fibroin Hydrogels Induced by Various Anionic Surfactants for Controlled Release of Curcumin
Authors: Chantong Nattakan , Damrongsakkul Siriporn , Ratanavaraporn Juthamas 
Published: May 2019

AbstractHydrogels based on Thai silk fibroin (SF) were prepared by the induction of various anionic surfactants including sodium octyl sulfate (SOS), sodium dodecyl sulfate (SDS), and sodium tetradecyl sulfate (STS), which have a similar chemical structure but different alkyl chain lengths and charges. The effects of anionic surfactant types and their concentrations on the gelation mechanism and time of SF were systematically investigated. We found that SDS and STS that have long alkyl chain lengths and high negative charges could accelerate the gelation of SF to occur within 14–42 min in a concentration‐dependent manner. SOS that has a short alkyl chain length and low negative charge slowly induced SF to gel at around 113–144 h. The mechanisms of SF gelation induced by these three anionic surfactants were supposed to be combination of hydrophobic and electrostatic interactions, as well as the self‐transition of beta sheets. The SF + STS hydrogels were further employed to encapsulate curcumin for the controlled release application. The SF + 0.09% wt. STS hydrogel encapsulating curcumin showed a slow rate of degradation while sustained the release of curcumin. This hydrogel can be applied as a minimal invasive injectable hydrogel or as a hydrogel for topical treatment of diseases.
Application of the Hydrophilic–Lipophilic Deviation Concept to Surfactant Characterization and Surfactant Selection for Enhanced Oil Recovery
Authors: Nguyen Thu T. , Morgan Carla , Poindexter Laurie , Fernandez Jorge 
Published: May 2019

AbstractThe hydrophilic–lipophilic deviation (HLD) concept has been demonstrated to be useful in determining characteristic curvature (Cc) of a surfactant. Cc is a surfactant parameter that reflects the hydrophobicity/hydrophilicity or the tendency of the surfactant to form microemulsions in an oil–water system. In order for the Cc value to be calculated, the formation of the optimum Winsor III microemulsion of oil and water systems under specific salinity and temperature conditions is required. Surfactant Cc values have been widely used to quantitatively screen and select a suitable surfactant in formulations for different application areas, especially enhanced oil recovery (EOR). The HLD concept is an effective tool for designing new surfactant molecules to meet the target Cc value for a specific formulation condition. The HLD equation indicates the dependence of a microemulsion system on the changes of various system parameters. This article demonstrates how the HLD equation can be derived in different ways depending on the characteristics of the surfactant to identify the proper experimental approach so that the Cc values of different types of surfactants can be determined. Three types of surfactants were studied, including nonionic alcohol ethoxylates, anionic alkyl propoxy ethoxy sulfates, and carboxylates. The application of the HLD concept to surfactant selection for EOR application was also demonstrated.
Botrytis cinerea differentially induces postharvest antioxidant responses in ‘Braeburn’ and ‘Golden Delicious’ apple fruit
Authors: Bui Tuyet TA , Wright Sandra AI , Falk Anders B , Vanwalleghem Tanja , Van Hemelrijck Wendy , Hertog Maarten LATM , Keulemans Johan , Davey Mark W 
Published: May 2019

AbstractBACKGROUNDThe fruit of two apple cultivars – ‘Braeburn’, which is susceptible to inoculation with Botrytis cinerea, and the less susceptible cv. ‘Golden Delicious’ – were investigated with respect to their response to inoculation with B. cinerea. Successful infection by B. cinerea leads to an oxidative burst and perturbation of plant redox homeostasis. To investigate the interaction between apple fruit and B. cinerea, antioxidant metabolism in fruit samples from sun‐exposed and shaded sides of different tissue types was measured over time.RESULTSThe sun‐exposed tissue of ‘Braeburn’ had higher initial levels of total vitamin C in the peel and phenolic compounds in the flesh than ‘Golden Delicious’, despite its greater susceptibility to gray mold. A substantial antioxidant response was recorded in diseased ‘Braeburn’ fruit 14 days after inoculation, which involved an elevated superoxide dismutase activity and ascorbate peroxidase activity, a progressive oxidation of total vitamin C, and a decrease in peroxidase activity and phenolic content. Disease development was slower on the sun‐exposed sides than on the shaded sides.CONCLUSIONThe two cultivars appeared to utilize different strategies to defend themselves against B. cinerea. ‘Golden Delicious’ almost entirely escaped infection. Preharvest exposure of apple fruit to high light / temperature stress appears to prepare them to better resist subsequent postharvest attack and disease. © 2019 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
Eliminate angular color shift in top‐emitting OLEDs through cavity design
Authors: Dong Chen , Fu Xiangyu , Amoah Stephen , Rozelle Adam , Shin Dong‐Hun , Salehi Amin , Mendes Juliana , So Franky 
Published: May 2019

AbstractOLEDs suffer from viewing angle dependent spectral shift due to microcavity effects. To address this issue, we introduce a novel top‐emitting OLED with a dielectric spacer that forms multiple cavity modes. The resulting device shows almost no color shift at different viewing angles.
Synthesis of Stannole‐Containing π‐Conjugated Polymers by Post‐Element Transformation of Organotitanium Polymer
Authors: Matsumura Yoshimasa , Sugihara Masato , Tan Sia‐Er , Sato Tatsuhiko , Hayashi Kohei , Nishiyama Hiroki , Zhou Wei‐Min , Inagi Shinsuke , Tomita Ikuyoshi 
Published: May 2019

AbstractThe synthesis of stannole‐2,5‐diyl‐containing π‐conjugated polymers by the post‐element transformation of a regioregular organotitanium polymer is described. For example, a 1,1‐diphenylstannole‐containing polymer is obtained in 83% yield by the reaction of a regioregular organotitanium polymer, which is prepared from 1,4‐bis(2‐ethylhexyloxy)‐2,5‐diethynylbenzene and a low‐valent titanium complex with diphenyltin dichloride at −50 °C to ambient temperature. The number‐average molecular weight and molecular weight distribution (Mn and Mw/Mn) of the stannole‐containing polymer are estimated as 4800 and 1.8, respectively. The obtained polymer is found to have the extended π‐conjugated backbone and relatively low‐lying lowest unoccupied molecular orbital (LUMO) energy level (−3.12 eV), which is supported by its UV–vis absorption spectrum and cyclic voltammetric (CV) analysis. In addition, the stannole‐containing polymer is found to be applicable to a chemosensor for fluoride anion where the color and photoluminescence intensity of the polymer solution exhibits a distinct change in the presence of a fluoride anion.
Zinc Nutrition and Inflammation in the Aging Retina
Authors: Gilbert Rosie , Peto Tunde , Lengyel Imre , Emri Eszter 
Published: May 2019

AbstractZinc is an essential nutrient for human health. It plays key roles in maintaining protein structure and stability, serves as catalytic factor for many enzymes, and regulates diverse fundamental cellular processes. Zinc is important in affecting signal transduction and, in particular, in the development and integrity of the immune system, where it affects both innate and adaptive immune responses. The eye, especially the retina‐choroid complex, has an unusually high concentration of zinc compared to other tissues. The highest amount of zinc is concentrated in the retinal pigment epithelium (RPE) (RPE‐choroid, 292 ± 98.5 µg g−1 dry tissue), followed by the retina (12362.2 µg g−1 dry tissue). The interplay between zinc and inflammation has been explored in other parts of the body but, so far, has not been extensively researched in the eye. Several lines of evidence suggest that ocular zinc concentration decreases with age, especially in the context of age‐related disease. Thus, a hypothesis that retinal function could be modulated by zinc nutrition is proposed, and subsequently trialled clinically. In this review, the distribution and the potential role of zinc in the retina‐choroid complex is outlined, especially in relation to inflammation and immunity, and the clinical studies to date are summarized.
Monodisperse Branched Molybdenum‐Based Bioactive Nanoparticles Significantly Promote Osteogenic Differentiation of Adipose‐Derived Stem Cells
Authors: Niu Wen , Guo Yi , Xue Yumeng , Chen Mi , Wang Min , Cheng Wei , Lei Bo 
Published: May 2019

AbstractAdipose‐derived stem cells (ADSCs) are considered to be ideal stem cell sources for bone‐tissue regeneration owing to their ease of collection and high activity. However, the regulation of osteogenic differentiation of ADSCs using biomaterials without adding growth factors is still not satisfactory. For the first time, molybdenum‐doped bioactive glass nanoparticles with a radial porous morphology (Mo‐rBGNs) are reported and their role in the osteogenic differentiation of ADSCs is investigated. The results show that Mo‐rBGNs exhibit radially porous and spherical morphology, relatively homogeneous particle size (200–400 nm), and excellent apatite‐forming bioactivity. They do not affect the proliferation of ADSCs, but significantly regulate their osteogenic differentiation and biomineralization. 5% Mo‐rBGNs significantly enhance the alkaline phosphatase activity and biomineralization ability and promote the osteogenic gene expressions of collagen I secretion and bone sialo protein in ADSCs. A reasonable and promising strategy for designing nanoscale bioactive materials with the excellent osteogenic ability for stem cell–based bone tissue regeneration is provided.
Tuning a Bisphenol A Lateral Flow Assay Using Multiple Gold Nanosystems
Authors: Lin Li‐Kai , Huang Peng‐Yuan , Dutta Sayan , Rochet Jean‐Christophe , Stanciu Lia A. 
Published: May 2019

AbstractAdvanced bisphenol A (BPA) lateral flow assays (LFAs) that use multiple nanosystems are reported. The assays use three nanosystems: gold nanostars, gold nanocubes, and gold nanorods, which are rarely applied in LFAs, compared with general gold nanoparticles that are referred to as gold nanospheres in this paper. These various nanosystems are bound to anti‐BPA antibodies and applied in LFAs to develop advanced BPA LFAs; the developed LFAs show differing BPA detection performance, as well as different visible colors, optical intensities, limits of detection, and application ranges. Advanced BPA LFAs that use multiple gold nano‐object shapes are successfully developed, and the geometry effects of diverse gold nanosystems coupled with anti‐BPA antibodies and the potential applications of regular BPA LFAs are explored.
Impacts of climate change and host plant availability on the global distribution of Brontispa longissima (Coleoptera: Chrysomelidae)
Authors: Zou Ya , Ge Xuezhen , Guo Siwei , Zhou Yuting , Wang Tao , Zong Shixiang 
Published: May 2019

AbstractBACKGROUNDThe coconut hispine beetle Brontispa longissima Gestro (Coleoptera: Chrysomelidae) is one of the most serious pests of the coconut palm, Cocos nucifera L. (Arecales: Arecaceae) and other palms. The invasion of B. longissima causes major economic and ecological losses worldwide. In this study, the impacts of climate change on the risk of spread were evaluated. CLIMEX was used to project its global potential distribution based on historical climate data (1987–2016) and simulated future climate data (2071–2100).RESULTSThe distribution of B. longissima included each continent under historical and future climate conditions. However, climate suitability was predicted to decrease in most tropical and subtropical regions under a climate change scenario. Temperature was a more important determinant of the climatic suitability of the pest than relative humidity or precipitation. The availability of host plants (Arecaceae) only had a slight impact on climate suitability in some regions.CONCLUSIONThe projected potential distribution of B. longissima will help to determine the impacts of climate change and will provide supportive information for the development of management strategies to reduce future economic and ecological losses. © 2019 Society of Chemical Industry
Research on reliability allocation technology for NC machine tool meta‐action
Authors: Li Yulong , Zhang Genbao , Wang Yongqin , Zhang Xiaogang , Ran Yan 
Published: May 2019

AbstractThe reliability of machine tool is getting more and more attention. Reasonable reliability allocation is beneficial to improve the inherent reliability of machine tool. However, the existing reliability allocation methods for machine tool have some limitations. For example, static part is selected as the reliability allocation object of machine tool, which cannot reflect the characteristics “function realized by motion”; factors affecting reliability allocation are not considered comprehensively, weights of experts are treated as the same, and the allocation results are not optimized or the impact of time on enterprise is neglected in the optimization. To solve these problems, a new multiobjective optimization reliability allocation method for machine tool is proposed in this paper. Firstly, the latest achievements about meta‐action are given, and meta‐action is set as the reliability allocation object. Secondly, more reasonable and comprehensive factors affecting reliability allocation are extracted. Thirdly, expert weight coefficient is brought to reduce the subjective impact of expert scoring. Fourthly, time factor is brought to make the optimized allocation results more reasonable and accurate. Finally, a numerical control (NC) machine tool made in China is taken as an example, with a comparison on the reliability allocation results of current methods and proposed method. The results verify the applicability, rationality, and accuracy of the proposed method, which lays a foundation for the subsequent study on the quality characteristics of machine tool based on meta‐action.
Charge‐dependent modulation of specific and nonspecific protein‐metal ion interactions in nanoelectrospray ionization mass spectrometry
Authors: Zheng Shihui , Yuan Siming , Hou Zhuanghao , Li Gongyu , Chen Yuting , Pan Yang , Liu Yangzhong , Huang Guangming 
Published: May 2019

RationalePrevious studies found that charge state could affect both specific and nonspecific binding of protein–metal ion interactions in nanoelectrospray ionization mass spectrometry (nESI‐MS). However, the two kinds of interactions have been studied individually in spite of the problem that they often coexist in the same system. Thus, it is necessary to study the effects of charge state on specific and nonspecific protein–metal ion interactions in one system to reveal more accurate binding state.MethodsThe HIV‐1 nucleocapsid protein (NCp7(31–55)) which can bind specifically and nonspecifically to Zn2+ served as the model to show the charge‐dependent protein–metal ion interactions. Hydrogen/deuterium exchange (HDX) and photodissociation (PD) were used to demonstrate that specific binding state was correlated with protein structure. In addition to NCp7(31–55), three other model proteins were used to investigate the reason for the charge‐dependent nonspecific binding.ResultsFor specific binding, we proposed that protein ions with different charge states had different conformations. The HDX results showed that labile protons in the NCp7(31–55)–Zn complex were exchanged in a charge‐state‐dependent way. The PD experiments revealed differential fragment yields for different charge states. For nonspecific binding, higher charge states had more Zn2+ additions, but less SO42− additions. The effects of charge states on nonspecific binding levels were entirely the opposite for Zn2+ and SO42−. These results could reveal that the nonspecific binding was caused by electrostatic interaction.ConclusionsFor specific binding, NCp7(31–55) with lower charge states have folding and undenatured structures. The binding states of lower charge states can better reflect more native binding states. For nonspecific binding, when multiple metal ions adduct to proteins, the proteins have more net positive charges, which tend to generate higher charge ions during electrospray.