bluuebunny/crossref_metadata_2025_split huggingface dataset:
Overexpression of HPIP as a biomarker for metastasis and prognosis prediction in endometrial cancer patients
Authors: Cheng Li , Zhao Tingting , Li Shiguo , Wang Yao , Fei Hui , Meng Fanling 
Published: June 2019

AbstractBackgroundHematopoietic pre‐B‐cell leukemia transcription factor (PBX)‐interacting protein (HPIP) has shown to be overexpressed in several human cancers. The purpose of this study was to explore the expression of HPIP in endometrial cancer (EC) and its associated effects on disease.MethodsA total of 113 EC patients at the Harbin Medical University Cancer Hospital between August 2011 and September 2012 were studied for immunohistochemistry analysis. HPIP expression was detected using real‐time reverse transcription PCR, Western blotting, and immunohistochemistry. Prognostic value of HPIP expression was examined using multivariate Cox regression analysis and Kaplan‐Meier method.ResultsThe result of Western blotting indicated that HPIP protein expression is significantly high in normal tissues compared to EC tissues (P < 0.001). The expression of HPIP was significantly associated with FIGO stage (P < 0.001), histological grade (P < 0.001), depth of myometrial invasion (P < 0.001), and lymph node metastasis (P = 0.033). Kaplan‐Meier analysis demonstrated that there was a significant difference in overall survival and disease‐free survival between the two groups of patients stratified by HPIP expression level (log‐rank, both P = 0.002). Patients with HPIP high expression had significantly shorter median survival time than those with HPIP low expression. Moreover, results of the multivariate analysis revealed that HPIP expression was an independent prognostic factor for predicting overall survival (P = 0.015) and disease‐free survival (P = 0.017) in patients with EC.ConclusionThe present study provides evidence that HPIP predicts EC progression and poor survival, highlighting its potential as a therapeutic target for EC.
MiR‐137 knockdown promotes the osteogenic differentiation of human adipose‐derived stem cells via the LSD1/BMP2/SMAD4 signaling network
Authors: Ma Xiaohan , Fan Cong , Wang Yuejun , Du Yangge , Zhu Yuan , Liu Hao , Lv Longwei , Liu Yunsong , Zhou Yongsheng 
Published: June 2019

AbstractMicroRNAs are a group of endogenous regulators that participate in several cellular physiological processes. However, the role of miR‐137 in the osteogenic differentiation of human adipose‐derived stem cells (hASCs) has not been reported. This study verified a general downward trend in miR‐137 expression during the osteogenic differentiation of hASCs. MiR‐137 knockdown promoted the osteogenesis of hASCs in vitro and in vivo. Mechanistically, inhibition of miR‐137 activated the bone morphogenetic protein 2 (BMP2)‐mothers against the decapentaplegic homolog 4 (SMAD4) pathway, whereas repressed lysine‐specific histone demethylase 1 (LSD1), which was confirmed as a negative regulator of osteogenesis in our previous studies. Furthermore, LSD1 knockdown enhanced the expression of BMP2 and SMAD4, suggesting the coordination of LSD1 in the osteogenic regulation of miR‐137. This study indicated that miR‐137 negatively regulated the osteogenic differentiation of hASCs via the LSD1/BMP2/SMAD4 signaling network, revealing a new potential therapeutic target of hASC‐based bone tissue engineering.
Metal‐based ionic liquid assisted synthesis of highly dispersed mesoporous Fe(III)/SiO2 for enhanced adsorption of antibiotics
Authors: Chao Yanhong , Jin Yan , Jiang Wenjuan , Chen LinLin , Li Xiaowei , Luo Jing , Pang Jingyu , Li Huaming , Zhu Wenshuai 
Published: June 2019

AbstractBACKGROUNDSilica‐based adsorbents have shown huge potential and bright perspective in adsorptive removal of antibiotic contaminants for water remediation. To further simplify the synthesis process, stabilize the doped metal and improve the adsorption capacity, a highly‐effective metal iron‐doped mesoporous silica (meso‐Fe(III)/SiO2) adsorbent was in situ one‐step synthesized under the strategy of using metal‐based ionic liquid [(C12H25)3NCH3]FeCl4 as template and iron source.RESULTSIron(III) species have been found highly dispersed in the confined space of meso‐SiO2 without pore blocking. Compared with the prototype and Fe‐impregnate meso‐SiO2 adsorbents, the meso‐Fe(III)/SiO2 exhibited significantly enhanced adsorptive removal of 95.6% and 93.8% for tetracycline (TC) and levofloxacin (LOF), respectively. The adsorption was controlled by diffusion processes and well described by pseudo‐second‐order kinetics equation and Langmuir isotherm model. Solution pH, co‐existing Na+ and Ca2+ exhibited significant impact on the adsorption process which is strongly related to π‐complexation, hydrophobic effect and electrostatic interactions.CONCLUSIONThe strategy of metal‐based ionic liquid assisted synthesis provide a promising approach to design and develop highly‐stable metal‐supported adsorbents. Furthermore, the excellent adsorption performance indicated the potential application of this meso‐Fe(III)/SiO2 in the removal of antibiotics from aqueous solutions. © 2019 Society of Chemical Industry
Myxedema coma accompanied by sick sinus syndrome and hypoventilation: A case report
Authors: Harada Ko , Murakami Shuntaro , Tokumasu Kazuki , Togawa Yu , Otsuka Fumio 
Published: June 2019

AbstractMyxedema coma is an emergency presentation of hypothyroidism, and cardiopulmonary manifestations of the disease are related to a high mortality rate. We herein report a case of myxedema coma accompanied by sick sinus syndrome requiring temporary cardiac pacing and hypercapnic respiratory failure in an 87‐year‐old woman. This case is unique because both of the cardiac and pulmonary manifestations were reversible and successfully treated with thyroid hormone replacement. Our case indicates that early detection of the condition and administration of levothyroxine are essential to improve the life‐threatening condition and avoid invasive treatment such as permanent pacemaker implantation or intubation.
The macrophage-related biomarkers sCD163 and sCD206 are released by different shedding mechanisms
Authors: Nielsen Marlene Christina , Andersen Morten Nørgaard , Rittig Nikolaj , Rødgaard-Hansen Sidsel , Grønbæk Henning , Moestrup Søren Kragh , Møller Holger Jon , Etzerodt Anders 
Published: June 2019

Abstract
The hemoglobin receptor CD163 and the mannose receptor CD206 are both expressed on the surface of human macrophages. Upon inflammatory activation, the receptors are shed from the macrophage surface generating soluble products. The plasma concentration of both soluble CD163 (sCD163) and soluble CD206 (sCD206) are increased in several diseases, including inflammatory conditions and cancer. Here, we show that in contrast to CD163, LPS-mediated shedding of CD206 in humans is slow and a result of indirect signaling. Although both sCD163 and sCD206 were increased in response to LPS stimulation in vivo, only CD163 was shed from LPS-stimulated macrophages in vitro. Although both sCD163 and sCD206 were released from cultured macrophages stimulated with zymosan and PMA, shedding of CD206 was generally slower and less efficient and not reduced by inhibitors against the major protease classes. These data indicate that CD163 and CD206 are shed from the macrophages by very different mechanisms potentially involving distinctive inflammatory processes.
Study on the structure optimization and anti‐hepatitis B virus activity of novel human La protein inhibitor HBSC11
Authors: Tong Shuangmei , Pan Jiaqian , Tang Jing 
Published: June 2019

AbstractIn our previous study, Methyl pyrazolo[1,5‐a] pyridine‐2‐carboxylate (HBSC11) was shown to combine with La protein, which conferred anti‐hepatitis B virus (HBV) effects. The purpose of this study was to optimize, synthesize, and evaluate the anti‐HBV activity of HBSC11. The methyl group of HBSC11 was substituted with hydrophobic, hydrophilic, and tricyclic groups to generate novel HBV inhibitors with desirable potency. On in vitro evaluation, several derivatives exhibited good anti‐HBV activity compared with control. In particular, compound 5a reduced the level of HBV antigen by approximately 50%, which was similar to the activity of entecavir. In a mouse model, 5a showed 98.9% inhibition rate for HBV DNA, 57.4% for HBsAg, and 46.4% for HBeAg; the corresponding rates in the control group were 90.8, 3.8, and 9.8%, respectively. In addition, prediction of binding modes and physicochemical properties showed that 5a formed hydrogen bonds with La protein and conformed well to the Lipinski's rule of five. Our results suggest that 5a is a potential new anti‐HBV drug.
TGF‐β1 enhances the activity of acid‐sensing ion channel in rat primary sensory neurons
Authors: Qiu Chun‐Yu , Liu Ting‐Ting , Wei Shuang , Zhou Yi‐Mei , Wu Lei , Jin Ying , Hu Wang‐Ping 
Published: June 2019

AbstractTransforming growth factor‐β1 (TGF‐β1) is an important member of multifunctional growth factor superfamily. It has been implicated in pain signaling, but little is known about the underlying mechanisms. Herein, we report that TGF‐β1 can exert a sustained enhancing effect on the functional activity of acid‐sensing ion channels (ASICs) in rat dorsal root ganglia (DRG) neurons. Pre‐application of TGF‐β1 increased the amplitude of proton‐gated currents in a dose‐dependent manner. Enhancement of ASIC currents lasted for more than 30 min although TGF‐β1 was treated once only. This sustained enhancement by TGF‐β1 could be blocked by extracellular treatment of selective TGF‐β receptor I antagonist SD‐208, and abolished by blockade of intracellular several non‐Smad‐signaling pathways. TGF‐β1 also sustainedly enhanced proton‐evoked spikes in rat DRG neurons. Moreover, peripheral pre‐treatment with TGF‐β1 dose‐dependently exacerbated nociceptive behaviors evoked by intraplantar injection of acetic acid through TGF‐β receptor I in rats. These results suggested that TGF‐β1 potentiated ASIC‐mediated electrophysiological activity and nociceptive behaviors, which revealed a novel mechanism underlying TGF‐β1 implicated in peripheral pain signaling by sensitizing ASICs.
Advances in axillary surgery for breast cancer 2019
Authors: Sener Stephen F. 
Published: June 2019

AbstractOver the last 25 years, modifications in systemic and radiation therapy for breast cancer have led to significant reductions in the risk of regional recurrence. During the same time, surgeons have demonstrated that it is safe to forego axillary dissections for patients with low burdens of axillary lymph node disease. When these advances are added to those by reconstructive microsurgeons, the promise of reducing morbidity rates from posttreatment lymphedema seems within our grasp.
Does sport event satisfaction remain stable over time?
Authors: Schnitzer Martin , Barth Michael 
Published: June 2019

AbstractVery little scholarly research analyzes sports event visitors' satisfaction using longitudinal study designs. Thus, this study investigates whether or not satisfaction with event services and products among sports event visitors is constant over time. Data were collected during the 2017 Crankworx Mountain Bike Festival staged in Innsbruck (Austria) and 6 months later, on an individual level (n = 161). The analyses showed no significant differences in satisfaction with the majority of event aspects during and after the festival. It is hoped that this research note will fuel the discussion within the sports tourism community with respect to satisfaction gained from sports events.
Topological Glycopolymers as Agglutinator and Inhibitor: Cyclic versus Linear
Authors: Liu Lei , Zhou Feng , Hu Jun , Cheng Xiaoxiao , Zhang Wei , Zhang Zhengbiao , Chen Gaojian , Zhou Nianchen , Zhu Xiulin 
Published: June 2019

AbstractCarbohydrates play an important role in biological processes for their specific interactions with proteins. Cyclic glycopolymers are promising to mimic the topology of natural macrocycle–biomacromolecules due to their unique architecture of lacking chain ends. To systematically study the effect of glycopolymer architecture on the interactions with protein, the cyclic glycopolymers bearing galactose side‐chain (cyclic PMAGn) with three degrees of polymerization (n= 14, 24, 47) are prepared for the first time. The cyclic PMAGnexhibits unique properties in agglutinating and inhibiting proteins in subsequent studies by comparison with the linear precursor with the same molecular weights. More impressively, the cyclic PMAGnhighlight the improved performance of cyclic architecture. For example, the cyclic PMAGnshows superior inhibition abilities to suppress amyloid formation from amyloid β protein fragment 1‐42 aggregation and block the specific interaction between bacteria and galactose‐modified surface compared to that of respective linear counterpart. This interesting finding suggests that the architecture of cyclic glycopolymers may be capable of optimizing the ability to bind or inhibit proteins in biological processes.
Impact of increasing age on functioning free muscle transplantation for facial palsy reconstruction
Authors: Lu Johnny C.‐Y. , Fang Frank , Lin Jerry T.‐K. , Chang Tommy N.‐J. , Chuang David C.‐C. 
Published: June 2019

AbstractBackgroundManagement of facial paralysis in older patients is controversial. The purpose of this study is to evaluate the impact of increasing age on functioning free muscle transplantation (FFMT).Patients and MethodsTwenty‐nine facial paralysis patients over 50 years old received FFMT. A separate group of patients aged 41–50 years old were included as control. Outcome assessments included use of the SMILE evaluation system for excursion, a cortical adaptation scoring system for brain plasticity, and a subjective satisfaction assessment score. Meta‐analysis of literature over the past 50 years was also performed to examine the impact of older age.ResultsThe mean change in excursion movement was 13.39 ± 5.49 mm. Weakest excursion was found in the oldest age group (11.74 ± 4.84 mm, p = .097), in concordance with the meta‐analysis. There was significant difference between the three different neurotizers (p = .036). Excursion and satisfaction score were significantly worse in the older cohort with the cross face‐nerve graft FFMT.ConclusionsFFMT is a viable option in the elderly patient group but performs weaker in excursion. The choice of neurotizer is dependent on the patient's goals and the suitable age at which the benefits outweigh the risks.
Resolution Enhancement in SEA XLOC for Heteronuclear NMR Long‐Range Correlation
Authors: Gyöngyösi Tamás , Kövér Katalin E. , Sørensen Ole W. 
Published: June 2019

AbstractIt is shown how the resolution in SEA XLOC NMR spectra for distinguishing between heteronuclear two‐ and three‐bond correlations for all 13C multiplicities can be improved by a modified experiment delivering absorptive profiles in the indirect dimension. The method is demonstrated with applications to ibuprofen and strychnine.
Outcome and prognostic factors in pediatric malignant peripheral nerve sheath tumors: An analysis of the European Pediatric Soft Tissue Sarcoma Group (EpSSG) NRSTS‐2005 prospective study
Authors: van Noesel Max M. , Orbach Daniel , Brennan Bernadette , Kelsey Anna , Zanetti Ilaria , de Salvo Gian Luca , Gaze Mark N. , Craigie Ross J. , McHugh Kieran , Francotte Nadine , Collini Paola , Bisogno Gianni , Casanova Michela , Ferrari Andrea 
Published: June 2019

AbstractBackgroundMalignant peripheral nerve sheath tumors (MPNST) are rare tumors of childhood. The role of standard chemotherapy in unresectable MPNST is still unclear. We report the outcome and prognostic factors in the EpSSG risk‐adapted prospective study for localized pediatric MPNST.MethodsPatients were stratified into four treatment groups defined by surgical resection, tumor size, and tumor grade (G): (a) surgery‐only group—resected tumors G1; (b) adjuvant radiotherapy group—R0/R1, G2 tumors; (c) adjuvant chemotherapy group—R0/R1, G3 tumors; and (d) neoadjuvant chemotherapy group—R2 resected tumors and/or nodal involvement. Chemotherapy consisted of four courses of ifosfamide‐doxorubicin and two courses of ifosfamide concomitant with radiotherapy (50.4‐54 Gy).ResultsOverall, the study included 51 patients. The 5‐year event‐free survival (EFS) and overall survival (OS) were 52.9% (95% confidence interval, 38.1‐65.8) and 62.1% (46.7‐74.3), respectively. The 5‐year EFS was 92% (56.6‐98.9) for treatment group 1 (N = 13), 33% (0.9‐77.4) for treatment group 2 (N = 4), 29% (4.1‐61.2) for treatment group 3 (N = 7), and 42% (23.1‐60.1) for treatment group 4 (N = 27). Response rate to chemotherapy (partial response + complete response) in patients with measurable disease was 46%. The presence of neurofibromatosis type 1 (NF1; 51% of patients) was an independent poor prognostic factor for OS and EFS.ConclusionThe outcome for patients with resectable MPNST was excellent. Standard ifosfamide‐doxorubicin for unresectable MPNST rendered the best reported outcome. Children with NF1 disease seem to have worse prognosis.
Toward triplet disilavinylidenes: A Hammett electronic survey for substituent effects on singlet‐triplet energy gaps of silylenes by DFT
Authors: Ayoubi‐Chianeh Mojgan , Kassaee Mohamad Z. 
Published: June 2019

AbstractTo reach for novel triplet silylenes, para‐ and meta‐R‐1,1‐diphenyldisilavinylidenes with various electron donating and withdrawing groups (EDGs and EWGs, respectively) are compared and contrasted using the B3LYP/6‐311++g** method, where para silylenes consist of 1H,
, 3OH,
, 5F, 6Cl,
, 8CHO,
, and
, whereas their meta isomers are
, 3′OH,
, 5′F, 6′Cl,
, 8′CHO,
, and
. The overall trend for triplet tendency of the latter group based on their singlet‐triplet energy gap (ΔEs‐t) is 3′OH >
> 1H >
>
> 8′CHO > 5′F > 6′Cl >
>
while that for the former, para series is
>
> 3OH >
> 5F > 6Cl > 1H >
> 8CHO >
. Plotting disilavinylidenes ΔEs‐t, against their corresponding Hammett substituent constants (σp or σm) shows rather significant ρ factors of −2.07 and −1.42 with good correlations of R2 = 0.98 and 0.63, respectively. The negative sign of the ρ indicates that the more EDGs have higher impacts on stabilizing or reaching for the triplet ground states, displaying
and
as two silylenes with triplet ground states. The EWGs increase electrophilicity (ω) making
(7.62 eV) the most electrophilic. In contrast, EDGs increase nucleophilicity (N) showing
(5.03 eV) as the most nucleophilic silylene. Moreover,
shows the highest proton affinity (PA = 388.13 kcal/mol), chemical potential (μ = −3.67 eV), energy of HOMO (EHOMO = −4.46 eV), dipole moment (5.44 debye), and the lowest band gap (ΔEH‐L = −1.59 eV), and electrophilicity (ω = 4.24 eV), with the lowest NBO charge on divalent Si atom (+0.129) among the scrutinized species.
Whole Genome Sequencing of Single Circulating Tumor Cells Isolated by Applying a Pulsed Laser to Cell‐Capturing Microstructures
Authors: Kim Okju , Lee Daewon , Chungwon Lee Amos , Lee Yongju , Bae Hyung Jong , Lee Han‐Byoel , Kim Ryong Nam , Han Wonshik , Kwon Sunghoon 
Published: June 2019

AbstractSingle cell analysis of heterogeneous circulating tumor cells (CTCs), by which the genomic profiles of rare single CTCs are connected to the clinical status of cancer patients, is crucial for understanding cancer metastasis and the clinical impact on patients. However, the heterogeneity in genotypes and phenotypes and rarity of CTCs have limited extensive single CTC genome research, further hindering clinical investigation. Despite recent efforts to build platforms that separate CTCs, the investigation on CTCs is difficult due to the lack of a retrieval process at the single cell level. In this study, laser‐induced isolation of microstructures on an optomechanically‐transferrable‐chip and sequencing (LIMO‐seq) is applied for whole genome sequencing of single CTCs. Also, the whole genome sequences and the molecular profiles of the isolated single cells from the whole blood of a breast cancer patient are analyzed.
Multicenter Investigation of Fungal Infections of Inflatable Penile Prostheses
Authors: Gross Martin S. , Reinstatler Lael , Henry Gerard D. , Honig Stanton C. , Stahl Peter J. , Burnett Arthur L. , Maria Pedro P. , Bennett Nelson E. , Kava Bruce R. , Kohler Tobias S. , Beilan Jonathan A. , Carrion Rafael E. , Munarriz Ricardo M. 
Published: June 2019

Abstract

Background
Fungal infections of inflatable penile prostheses (IPPs) are inadequately understood in the literature.

Aim
To review a multi-institution database of IPP infections to examine for common patient and surgical factors related to IPP fungal infections.

Methods
This is a retrospective Institutional Review Board–approved analysis of 217 patients at 26 institutions who underwent salvage or device explant between 2001 and 2018. Patient data were compiled after an extensive record review.

Outcomes
26 patients (12%) with fungal infections were identified.

Results
23 of 26 patients (83%) with a fungal IPP infection were either diabetic or overweight. 15 patients had undergone primary IPP implantation, and the other 11 had previously undergone an average of 1.7 IPP-related surgeries (range 1–3; median 2). The average age at implantation was 63 years (range 31–92; median 63). 18 of the 26 patients with fungal infection had diabetes (69%), with a mean hemoglobin A1c (HbA1c) value of 8.4 (range 5.8–13.3; median 7.5). Twenty-two patients (85%) were overweight or obese. The mean body mass index for all patients was 30.1 kg/m2 (range 23.7–45 kg/m2; median 28.4 kg/m2), and that for diabetic patients was 30.8 kg/m2 (range 24.1–45 kg/m2, median 29.7 kg/m2). Ninety-one percent of implants were placed with intravenous antibiotics, consistent with current American Urological Association guidelines: an aminoglycoside plus first- or second-generation cephalosporin or vancomycin or ampicillin/sulbactam or piperacillin/tazobactam. 65% (17 of 26) of infected IPPs had only fungal growth in culture. No patient had concomitant immunosuppressive disease or recent antibiotic exposure before IPP implantation.

Clinical Implications
More than two-thirds of the fungal infections occurred in diabetic patients and 85% occurred in overweight or obese patients, suggesting that antifungal prophylaxis may be appropriate in these patients.

Strengths & Limitations
This is the largest series of fungal infections reported to date in the penile prosthesis literature. The overall number of such cases, however, remains small.

Conclusion
Fungal infections represent 12% of all penile prosthesis infections in our series and were seen mostly in diabetic or overweight patients, who may benefit from antifungal prophylaxis.