bluuebunny/crossref_metadata_2025_split huggingface dataset:
Destructive twisting of neutral metalloproteases: the catalysis mechanism of the Dispase autolysis‐inducing protein from Streptomyces mobaraensis DSM 40487
Authors: Fiebig David , Storka Juliana , Roeder Markus , Meyners Christian , Schmelz Stefan , Blankenfeldt Wulf , Scrima Andrea , Kolmar Harald , Fuchsbauer Hans‐Lothar 
Published: September 2018

The Dispase autolysis‐inducing protein (DAIP) is produced by Streptomyces mobaraensis to disarm neutral metalloproteases by decomposition. The absence of a catalytic protease domain led to the assumption that the seven‐bladed β‐propeller protein DAIP causes structural modifications, thereby triggering autolysis. Determination of protein complexes consisting of DAIP and thermolysin or DAIP and a nonfunctional E138A bacillolysin variant supported this postulation. Protein twisting was indicated by DAIP‐mediated inhibition of thermolysin while bacillolysin underwent immediate autolysis under the same conditions. Interestingly, an increase in SYPRO orange fluorescence allowed tracking of the fast degradation process. Similarly rapid autolysis of thermolysin mediated by DAIP was only observed upon the addition of amphiphilic compounds, which probably amplify the induced structural changes. DAIP further caused degradation of FITC‐labeled E138A bacillolysin by trypsin, as monitored by a linear decrease in fluorescence polarization. The kinetic model, calculated from the obtained data, suggested a three‐step mechanism defined by (a) fast DAIP–metalloprotease complex formation, (b) slower DAIP‐mediated protein twisting, and (c) fragmentation. These results were substantiated by crystallized DAIP attached to a C‐terminal helix fragment of thermolysin. Structural superposition of the complex with thermolysin is indicative of a conformational change upon binding to DAIP. Importantly, the majority of metalloproteases, also including homologs from various pathogens, are highly conserved at the autolysis‐prone peptide bonds, suggesting their susceptibility to DAIP‐mediated decomposition, which may offer opportunities for pharmaceutical applications.DatabasesThe atomic coordinates and structure factors (PDB ID: 6FHP) have been deposited in the Protein Data Bank (http://www.pdb.org/).EnzymesAureolysin, EC 3.4.24.29; bacillolysin (Dispase, Gentlyase), EC 3.4.24.28; lasB (elastase), EC 3.4.24.4; subtilisin, EC 3.4.21.62; thermolysin, EC 3.4.24.27; transglutaminase, EC 2.3.2.13; trypsin, EC 3.4.21.4; vibriolysin (hemagglutinin(HA)/protease), EC 3.4.24.25.
European Society of Endodontology position statement: External Cervical Resorption
Authors:   European Society of Endodontology (ESE) developed by:, Patel S. , Lambrechts P. , Shemesh H. , Mavridou A. 
Published: September 2018

AbstractThis Position Statement represents the consensus of an expert committee convened by the European Society of Endodontology (ESE) on External Cervical Resorption (ECR). The statement is based on current clinical and scientific evidence as well as the expertise of the committee. The primary aim is to provide a current authoritative position on the aetiology, histopathology, clinical presentation and management of ECR, and also to highlight areas where there is minimal evidence. Previously published review articles provide more detailed background information and the basis for this position statement (International Endodontic Journal 51, 1205, 2018, International Endodontic Journal 51, 1224, 2018). It is intended that this position statement will be updated at appropriate intervals, as further evidence emerges.
Identification and expression analysis of the Spodoptera exigua neuropeptidome under different physiological conditions
Authors: Llopis‐Giménez A. , Han Y. , Kim Y. , Ros V. I. D. , Herrero S. 
Published: September 2018

AbstractNeuropeptides are small signalling molecules acting as neurohormones, neurotransmitters and neuromodulators. Being part of the chemical communication system between cells within an organism, they are involved in the regulation of different aspects of animal physiology and behaviour such as feeding, reproduction, development and locomotion. Transcriptomic data from larval and adult tissues have been obtained and mined to generate a comprehensive neuropeptidome for the polyphagous insect pest Spodoptera exigua. Sixty‐three neuropeptides have been identified and described based on their tissue specificity and their regulation in response to different abiotic perturbations. Expression analyses have identified those neuropeptides involved in ingestive and digestive behaviour of S. exigua larvae and revealed a general pattern of upregulation in the midgut during larval starvation. Our results represent a comprehensive neuropeptidome of a lepidopteran species that will be highly relevant to future studies and provide novel information of the insect’s perception of its environment.
Aggressive prevention and preemptive management of vascular complications after pediatric liver transplantation: A major impact on graft survival and long‐term outcome
Authors: Grimaldi Chiara , di Francesco Fabrizio , Chiusolo Fabrizio , Angelico Roberta , Monti Lidia , Muiesan Paolo , de Ville de Goyet Jean 
Published: September 2018

AbstractVascular complications are a major cause of patient and graft loss after LTs. The aim of this study was to evaluate the effect of a multimodal perioperative strategy aimed at reducing the incidence of vascular complications. A total of 126 first isolated LTs—performed between November 2008 and December 2015—were retrospectively analyzed. A minimum follow‐up period of 24 months was analyzable for 124/126 patients (98.4%). The aggressive preemptive strategy consisted of identifying and immediately managing any problem and any abnormality in the vascular flow, in any of the hepatic vessels, and at any time after the liver graft revascularization. As a result, with a median follow‐up of 57 months (3‐112 months), not a single graft has been lost from vascular or biliary problems. The actuarial 8‐year graft survival is 96.5%. These results have shown that a combination of technical attention, medical prevention, an early diagnosis, and rapid interventions reduced the negative impact of vascular problems on the outcome of both grafts and patients.
Combined treatment with oestradiol benzoate, d‐cloprostenol and oxytocin permits cervical dilation and nonsurgical embryo recovery in ewes
Authors: da Fonseca Jeferson Ferreira , Zambrini Fabiana Nunes , Guimarães José Domingos , Silva Marcio Roberto , Oliveira Maria Emilia Franco , Brandão Felipe Zandonadi , Bartlewski Pawel Mieczyslaw , Souza‐Fabjan Joanna Maria Gonçalves 
Published: September 2018

ContentsThis study examined the feasibility of transcervical embryo recovery after the hormonal treatment to induce cervical dilation, following the 7‐day oestrous synchronization protocol in multiparous Santa Inês ewes. A total of 23 cyclic ewes received two doses of 37.5 μg of d‐cloprostenol by latero‐vulvar route 7 days apart. After the second injection of d‐cloprostenol, the ewes were checked for oestrus (every 12 hr) and then mated by fertile rams throughout the oestrous period. All ewes received 37.5 μg of d‐cloprostenol (latero‐vulvar) and 1 mg of oestradiol benzoate by either intramuscular (EBim group; n = 12) or intravaginal (EBivg group; n = 11) route 16 hr before embryo flushing. Twenty minutes before the flushing, 50 IU of oxytocin were administered intravenously. The oestrous response (i.e., the percentage of ewes that showed signs of oestrous behaviour after the second d‐cloprostenol injection) was 91.3% (21/23). The proportion of successfully penetrated ewes (81.8% compared with 80.0%), the mean duration of embryo flushing (24.7 ± 2.0 min compared 26.2 ± 1.9 min), the flushing fluid recovery rate (94.8 ± 1.3% compared with 91.0 ± 2.9%) and the average number of structures recovered per ewe (0.5 ± 0.4 compared with 0.8 ± 0.4) did not vary (p > 0.05) between the EBim and EBivg groups. Viable embryos were recovered from 41.2% (7/17) of successfully penetrated ewes. It can be concluded that nonsurgical (i.e., transcervical) embryo collection can be performed in oestrous‐synchronized Santa Inês ewes pretreated with d‐cloprostenol, oxytocin and oestradiol benzoate, with the latter hormone administered by either the intramuscular or intravaginal route.
Effect of frame rate capture frequency on sperm kinematic parameters and subpopulation structure definition in boars, analysed with aCASA‐Mot system
Authors: Valverde Anthony , Madrigal Mónica , Caldeira Carina , Bompart Daznia , de Murga Javier Núñez , Arnau Sandra , Soler Carles 
Published: September 2018

ContentsMotility is the most widely used indicator of sperm quality. Computer‐Assisted Semen Analysis (CASA) allows the objective evaluation of sperm motility parameters.CASAtechnology is a common tool to predict semen doses in farm animal reproduction. The kinds of video cameras used until now for image acquisition have presented limited frame rates (FR), which have a negative influence on the quality of the obtained data. The aim of the present work was to define the optimal frame rate for a correct evaluation of boar sperm motility and its subpopulation structure. Eighteen ejaculates from nine mature boars of the Pietrain breed were used. Using theISAS®v1CASA‐Mot system, with a video camera working up to 200 Hz, sixFRs (25, 50, 75, 100, 150 and 200 fps) were compared.ISAS®D4C20 counting chambers, warmed to 37°C, were used.FRaffected all the kinematic parameters, with curvilinear velocity (VCL) andBCFthe most sensitive ones. All the parameters showed differences among animals. Non‐linear correlation showed the asymptotic level forVCLat 212 fps, being the highestFRfor all the parameters. For future studies based just on progressive motility, almost 100 fpsFRfor 0.5 s must be used, while when kinematics must be considered, almost 212 fps for one‐second should be analysed. Three principal components were obtained (velocity, progressivity and oscillation), being similar at 50 and 200 fps. Cells were grouped in four subpopulations but with different kinematic and cellular distribution at bothFRs.
A study on ghrelin and LH secretion after short fasting and on ghrelin levels at perioestrual period in dairy cattle
Authors: Chouzouris Thomas Markos , Dovolou Eleni , Rekkas Constantinos A. , Georgoulias Panagiotis , Athanasiou Lambrini V. , Amiridis Georgios S. 
Published: September 2018

ContentsIn two experiments, we studied (a) the changes of LH secretion in heifers under different feeding schedules and (b) total ghrelin concentration at oestrus in cows and heifers. In experiment one, synchronized heifers were allocated in three groups (R, regularly fed controls; F, fasted; and F‐F fasted‐fed). One day after the completion of the oestrous induction protocol, group F and F‐F animals stayed without feed for 24 hr; thereafter, feed was provided to R and F‐F cattle; 2 hr later, GnRH was administered to all animals. Blood samples were collected for ghrelin, progesterone, LH and cortisol concentrations. Fasting caused increased ghrelin concentrations in groups F and F‐F, while in response to GnRH, LH surge was significantly attenuated in groups F and F‐F compared to R. In experiment 2, lactating cows and heifers were used. On day 9 of a synchronized cycle, PGF2α was administered, and blood samples were collected twice daily until the third day after oestrus and analysed for progesterone, estradiol, ghrelin, glucose and BHBA concentrations. No difference was recorded between groups in steroids and BHBA concentrations. In comparison to mid‐luteal values, ghrelin concentrations significantly increased at perioestrual period in cows, but not in heifers. This study provides evidence that starving‐induced elevated ghrelin concentrations can have suppressing effect on LH secretion, even after ghrelin's restoration to basal values and that during oestrus, ghrelin secretion is differently regulated in cows and heifers, likely being independent from oestradiol concentrations. Further research is required to identify the determining factors that drive the different regulation of ghrelin secretion in cows and heifers.
Lifestyle interventions in prevention and comprehensive management of COPD
Authors: Ambrosino Nicolino , Bertella Enrica 
Published: September 2018

Chronic respiratory diseases are among the four major human chronic diseases. Tobacco smoke as well as environmental pollutants, infections, physical activity and nutritional status play a role in the prevalence, development and/or progression of chronic obstructive pulmonary disease (COPD).Changes in lifestyle are possible and may be beneficial in prevention and comprehensive management of COPD. Population-level interventions aimed at early diagnosis, promotion of vaccinations and prevention of infections, and reductions in smoking, environmental pollutants, physical inactivity, obesity and malnutrition may increase the number of life-years lived in good health.Educational aimsTo improve awareness of the influence of lifestyle on natural history of COPD.To describe the effects of some interventions to modify lifestyle in prevention and management.To provide information on the main clinical results.To define recommendations and limitations.
Zwei-Reiche-Lehre und Widerstand gegen die Staatsgewalt. Entfaltung einer Bemerkung Bonhoeffers
Authors: Ohly Lukas 
Published: September 2018

Abstract
A passage from one of Bonhoeffer’s prison letters hints at a diversification of Luther’s doctrine of the two kingdoms. Thus Bonhoeffer solves a dilemma: On the one hand, the doctrine of the two kingdoms does not support rogue regimes. On the other hand, Bonhoeffer escapes the danger of dissolving political justice by religious fanaticism. The solution, which is recon­structed in this essay, consists of a non-principal, but nevertheless evident situation of decision in favor of resistance. Any possible objections against this proposition are countered by the theological phenomenon of »Widerfahrnis« of human experience.
Crystal structure of 5,5′-dimethoxy-2,2′-[1,1′-(ethylenedioxydinitrilo)diethylidyne]diphenol, C20H24N2O6
Authors: Ren Zong-Li , Wang Lan , Liu Ling-Zhi , Li Xiang-Qian , Dong Wen-Kui 
Published: September 2018

Abstract
C20H24N2O6, triclinic, P1̄, a = 7.1389(5) Å, b = 9.4977(7) Å, c = 14.6456(10) Å, α = 105.118(3)°, β = 101.188(2)°, γ = 94.739(3)°, Z = 2, V = 931.08(11) Å3, R
gt(F) = 0.0564, wR
ref(F
2) = 0.1646, T = 153(2) K.
Crystal structure of methyl 4-(4-hydroxy-3-nitrophenyl)-2-methyl-5-oxo-1,4,5,6,7,8-hexahydroquinoline-3-carboxylate, C18H18N2O6
Authors: Gou Wei , Peng Ling , Li Bo , Yang Qinghua , Cao Lewei 
Published: September 2018

Abstract
C18H18N2O6, monoclinic, P21/n (no. 14), a = 7.4809(14) Å, b = 13.082(2) Å, c = 16.870(3) Å, β = 93.440(4)°, V = 1648.0(5) Å3, Z = 4, R
gt(F) = 0.0433, wR
ref(F
2) = 0.1279, T = 296(2) K.
Oxygen content and defect structure of the perovskite La0.5Ba0.5CoO3–δ
Authors: Malyshkin D. A. , Novikov A. Yu. , Tsvetkov D. S. 
Published: September 2018

Perovskite-type complex oxide La0.5Ba0.5CoO3–δ, promising cathode material for solid oxide fuel cells and precursor for synthesis of double perovskite LaBaCo2O6–δ, was prepared as a single-phase material. Its oxygen content was measured by two independent techniques in the temperature range 1000–1100 °C and at oxygen partial pressures corresponding to the stability field of cubic phase. The defect chemistry of this material was studied using the measured δ=f(pO2,T) dependences. The defect structure model based on the localized nature of the electronic defects was proposed and successfully verified.
Matematik Öğretmeni Adaylarının Geometrik Şekiller Üzerine Akıl Yürütme Becerilerinin Üniversite ve Sınıf Düzeyi Değişkenleri Açısından İncelenmesi
Authors: İLHAN Aziz , ASLANER Recep 
Published: September 2018

Bu çalışmada, matematik
öğretmeni adaylarının geometrik şekiller üzerine akıl yürütme becerileri
üniversite ve sınıf düzeyi değişkenleri açısından İncelenmiştir. Bu nedenle
çalışmada tarama türlerinden ilişkisel tarama deseni kullanılmıştır.
Araştırmanın katılımcılarını Doğu ve Güneydoğu Anadolu Bölgelerinde bulunan
iki üniversitede 2015-2016 güz döneminde öğrenim görmekte olan 266 matematik
öğretmeni adayı oluşturmaktadır. Veri
toplama aracı olarak araştırmacı tarafından geliştirilmiş olan GŞAYB testi
kullanılmıştır. Katılımcıların sınıf düzeyleri temel alınarak Frekans (f),
Yüzde (%) ve Ortalama () değerlerinden, verilerin homojen dağılıp
dağılmadıklarına göre; ANOVA, Tukey ve t-testlerinden yararlanılmıştır. Elde edilen verilere göre matematik
öğretmeni adaylarının geometrik şekiller üzerine akıl yürütme becerilerinin
orta düzeyde olduğu, bu düzeyin sınıflar arasında anlamlı farklılığa sahip
olduğu görülmüştür. Ayrıca üniversite değişkenine göre de anlamlı farklılık
gösterdiği sonucu elde edilmiştir. Araştırmadan elde edilen bulgular
neticesinde matematik öğretmeni adaylarının geometrik şekiller üzerine akıl
yürütme beceri düzeylerini geliştirecek öğretim yöntemlerini içeren deneysel
çalışmaların yapılabileceği tavsiye edilmiştir.
Asociación entre los niveles de déficit de base y la aparición de disfunción orgánica múltiple en pacientes con preeclampsia severa admitidas en la clínica gestión salud s.a.s (Cartagena) entre el 1 de enero de 2014 hasta 31 diciembre 2016
Authors: Buelvas Villalba Melkis , Jaraba Coronado Rafael 
Published: September 2018

Objetivo: Determinar la asociación entre los niveles de déficit de base y la aparición de disfunción orgánica en pacientes con preeclampsia severa admitidas en la Clínica Gestión salud S.A.S (Cartagena) entre el 1 de enero de 2014 hasta 31 Dic 2016. Método: La Pseudomonas Aerugiosa es una bacilo gramnegativos, con diámetros de 1 - 3 mm de longitud, aerobio obligado, oxidasa positiva que crece a 37-42 °C, de habita variable (tierra, agua, plantas y animales). Se caracteriza por formar colonias redondeadas, lisas con olor a uvas y de variable color según el pimento producidos por la cepa color verde-azulado (piocinina y pioverdina), rojo oscuro (piorrubina) y negro (piomelanina). Resultados: Mediante un análisis de regresión logística se exploró la fuerza de asociación entre tener un déficit de base menor o igual a -5 y el desarrollo de DOM encontrándose una asociación estadísticamente significativa. Tener un déficit de base menor o igual a -5 aumentaba la probabilidad de desarrollar DOM con una OR de 3,73 (IC 95% 1,28 a 12,05 p=0,0197). Conclusión: Valores de DB menores a -5, es decir, tener un mayor DB se asocia a un aumento en la probabilidad de tener DOM. En este grupo de pacientes también son más frecuentes la disfunción hepática, la disfunción de la coagulación y el síndrome de HELLP.
Caracterización de infecciones en adultos por staphylococcus aureus meticilino-resistente (SAMR) en una institución de salud de IV nivel, Barranquilla 2016
Authors: Maestre Zabala Sily Savel del Carmen , Henao Cabarcas Luis Carlos 
Published: September 2018

Objetivo: Determinar las características epidemiológicas, microbiológicas y clínicas de infecciones causadas por Staphylococcus aureus meticilino-resistente en pacientes adultos hospitalizados en una institución de IV nivel de complejidad en el año 2016. Método: La obtención de datos se realizó mediante la utilización de la base de datos del laboratorio de microbiología y la revisión de historias clínicas sistematizada de pacientes que fueron hospitalizados en el periodo enero a diciembre 2016 con reporte de cultivo positivo para Staphylococcus aureus meticilino-resistente, en el Hospital Universitario ESE Cari. Resultados: Al relacionar las variables edad, sexo, presencia de comorbilidades, qSOFA, estancia hospitalaria y días con antibiótico, se encontró que hubo diferencias estadísticamente significativas entre qSOFA y mortalidad (Fisher<0,05), y aunque en el resto de casos las diferencias no resultaron significativas (en gran parte debido al tamaño muestral y a que solamente 6 sujetos fallecieron), se destaca el hecho que todos aquellos que presentaron al menos una comorbilidad fallecieron, y que esta fue mayor en las mujeres, en los que tuvieron estancias hospitalarias más prolongadas y mayor número de días con antibióticos. Conclusión: Los resultados del presente estudio muestran un alto número de casos de S. Aureus MR. La mayoría de ellos eran adultos jóvenes, con estancias hospitalarias variables, en los que se empleó terapia combinada.
Analysis of Lecturers’ Work Performance Based on Pedagogy Competence and Work Motivation
Authors: . Misdalina , Asmawi Moch. , Sujanto Bedjo 
Published: September 2018

Lecturers’ work performance is one of the most crucial factors in the attainment of success of the university’s vision. Thus, extra attempts need to be done in enhancing factors that influence the work performance. The objective of this research is to determine the effect of pedagogy competence and work motivation toward lecturers’ work performance. The research is conducted using the quantitave approach. Research samples selected as much as 172 permanent lecturers of Universitas Persatuan Guru Republik Indonesia (UPGRI) Palembang. Foundation research data are collected through test and questionnaire. Further, data collected are analyzed using t-test and path analysis. Based on the research, it is found that: (1) there is direct positive effect of the pedagogy competence toward work performance; (2) there is direct positive effect of the work motivation toward work performance;(3) there is direct positive effect of the pedagogy competence toward work motivation. The conclusion is that work motivation needs to be firstly improved than the pedagogy competence so as to level up the work performance of UPGRI Palembang lecturers.

Keywords: Work performance, Pedagogy competence, Work motivation.
OPPORTUNITIES OF LOW-DOSE DIGITAL FLUOROGRAPHY IN DIAGNOSIS OF PHENOTYPIC VARIANTS OF CHRONIC OBSTRUCTIVE PULMONARY DISEASE
Authors: Gorbunov N. A. , Dergilev A. P. , Kochura V. I. , Manakova Ya. L. , Volchenko S. N. , Sudarkina A. V. 
Published: September 2018

Objective. To determine the opportunities of low-dose digital fluorography (LDDF) in differential diagnosis of phenotypic variants of chronic obstructive pulmonary disease (COPD).Material and methods. There were 107 patients with clinically diagnosed COPD of varying severity examined. The average age of the patients was 51.8±1.5 years (46–59 years). All patients for LDDF of the chest in the frontal projection in the inspiratory and expiratory phase of respiration were undergone.Results. The electron optical density of the lungs was determined in the upper, middle and lower zones of both lungs. As a result in patients with a predominance of emphysematous variant of COPD (n=15) the most characteristic radiological symptom was lung hyperventilation (14% of 107 patients) and inspiratory electron optical density value was 748.18±4.72 optical density units (ODU). In patients with a predominance of bronchitic variant of COPD (n=43) the most common radiological symptom was the presence of symptom amplification and deformation of lung pattern (40% of 107 patients) and inspiratory electron optical density value was 668.04±12.26 ODU. For patients with mixed phenotypic variant of COPD (n=49) it was characterized by a combination of X-ray symptom amplification and deformation of lung pattern with lung emphysema (46% of 107 patients); the average value of inspiratory electron optical density value was 815.24±17.25 ODU.Conclusion. The technique of LDDF can detect X-ray symptoms and determine inspiratory and expiratory electron optical density of the lungs in patients with COPD that allows optimizing the differential diagnosis of phenotypic variants of chronic obstructive pulmonary disease.