bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clustering of hepatitis C virus antibody positivity within households and communities in Punjab, India
Authors: Trickey A. , Sood A. , Midha V. , Thompson W. , Vellozzi C. , Shadaker S. , Surlikar V. , Kanchi S. , Vickerman P. , May M. T. , Averhoff F. 
Published: October 2019

AbstractTo better understand hepatitis C virus (HCV) epidemiology in Punjab state, India, we estimated the distribution of HCV antibody positivity (anti-HCV+) using a 2013–2014 HCV household seroprevalence survey. Household anti-HCV+ clustering was investigated (a) by individual-level multivariable logistic regression, and (b) comparing the observed frequency of households with multiple anti-HCV+ persons against the expected, simulated frequency assuming anti-HCV+ persons are randomly distributed. Village/ward-level clustering was investigated similarly. We estimated household-level associations between exposures and the number of anti-HCV+ members in a household (N= 1593 households) using multivariable ordered logistic regression. Anti-HCV+ prevalence was 3.6% (95% confidence interval 3.0–4.2%). Individual-level regression (N= 5543 participants) found an odds ratio of 3.19 (2.25–4.50) for someone being anti-HCV+ if another household member was anti-HCV+. Thirty households surveyed had ⩾2 anti-HCV+ members, whereas 0/1000 (P< 0.001) simulations had ⩾30 such households. Excess village-level clustering was evident: 10 villages had ⩾6 anti-HCV+ members, occurring in 31/1000 simulations (P= 0.031). The household-level model indicated the number of household members, living in southern Punjab, lower socio-economic score, and a higher proportion having ever used opium/bhuki were associated with a household's number of anti-HCV+ members. Anti-HCV+ clusters within households and villages in Punjab, India. These data should be used to inform screening efforts.
Density and habitat associations of the Altamira YellowthroatGeothlypis flavovelatain Veracruz, Mexico: an endemic vulnerable species
Authors: HERNÁNDEZ-SÁNCHEZ MAURICIO , GONZÁLEZ-GARCÍA FERNANDO , ROJAS-SOTO OCTAVIO R. , MACGREGOR-FORS IAN 
Published: October 2019

SummaryThe Altamira YellowthroatGeothlypis flavovelatais endemic to north-eastern Mexico, with a restricted distribution due to the spatial arrangement of its major habitat: wetlands. Given the lack of information regarding this vulnerable and endemic landbird, here we describe and analyse the sites where we recorded it in Northern Veracruz, as well as its population density, and natural history information. Our results show that the average density of this endemic yellowthroat is 1.006 ind/ha, with more individuals recorded in Tecolutla when compared to Tuxpan. We found a strong association between the Altamira Yellowthroat and southern cat-tailTypha domingensis, although we found scenarios under which the presence of the cat-tail was not a determinant of Altamira Yellowthroat presence. In light of the strong anthropogenic pressures on wetlands in the region, the Altamira Yellowthroat has become highly vulnerable. Thus, if we aim to preserve this endemic species, together with other wetland-dependent species, it is crucial to moderate –and even stop– human pressures on these ecosystems and mitigate past damages.
From periphery to centre and back again: elite transformations in Mechelen (fourteenth to sixteenth centuries)
Authors: Buylaert Frederik 
Published: October 2019

AbstractThis article explores the social history of the political elites of Mechelen, a town that evolved from a seigneurial enclave within the duchy of Brabant to thede factocapital of the Burgundian–Habsburg Low Countries between the 1470s and 1530. Proceeding from a quantitative analysis of lists of aldermen, fiscal registers and epitaphs, the article argues that the short-lived functioning of Mechelen as a capital city had great impact on its ruling classes. Mechelen was traditionally ruled by a coalition of craft guilds and prominent citizens, but the latter reoriented their social networks to the court elite, as the latter's presence supercharged pre-existing trends towards ennoblement among the urban elite.
Development and validation of the Dental Nutrition Knowledge Competency Scale for low-income women
Authors: Sachdev Prageet K , Freeland-Graves Jeanne , Babaei Mahsa 
Published: October 2019

AbstractObjective:To develop and validate a Dental Nutrition Knowledge Competency Scale to assess dental health-related nutrition knowledge of low-income women.Design:This is a cross-sectional study. A literature search for foods/dietary practices related to dental caries was conducted and the items were incorporated into an initial questionnaire. A panel of ten nutrition experts evaluated it for its content, readability and relevance, and a focus group of six low-income women determined its readability and comprehension. Then the questionnaire was administered to 150 low-income women. Construct validity was evaluated by item difficulty, item discrimination and factor analysis. Internal consistency reliability was tested via Cronbach’s α. In a sub-sample of forty women, test–retest reliability was established. Paired-sample t tests were conducted to examine differences between test scores at the two time points, 2 weeks apart.Settings:Community centres in low-income housing in Central Texas, USA.Participants:A total of 150 low-income women, aged 18–50 years; annual household income <250 % of the federal poverty level.Results:Item difficulty and discrimination analysis resulted in elimination of eight questions. Factor analysis identified twenty-four items that loaded on three factors related to knowledge. These included foods/dietary practices that affect dental caries, added sugars in foods and on food labels, and recommended frequency of oral hygiene practices. The subscales and the completed scale exhibited good internal consistency (mean 0·7 (sd 0·97)) and test–retest reliability (mean 0·8 (sd 0·013)).Conclusions:The Dental Nutrition Knowledge Competency Scale is a validated and reliable instrument to assess nutrition knowledge related to dental health in low-income women.
Fabrication of anthropomorphic phantoms for use in total body irradiations studies
Authors: Monzari Shaghayegh F. , Geraily Ghazale , Hadisi nia Tahereh , Salmanian Soraya , Toolee Heydar , Farzin Mostafa 
Published: October 2019

AbstractPurpose:The aim of this study was to produce a low-cost anatomical model of adult male including lower limbs to evaluate the three-dimensional dose distribution for dosimetry measurements, especially in total body irradiation (TBI) and total skin electron therapy (TSET).Materials and methods:Computed tomography (CT) scan images of the atomic energy organisation RANDO phantom and lower limb CT scan images of 20 healthy persons were averaged. Selections of different body tissues substitute materials and phantom validation were performed according to previous studies worked on construction of radiation therapy phantoms.Results:The dosimetry aspect of the selected substitute materials from all considered methods showed that they were in good agreement with real human tissue, especially bone, with a percentage error of 0·5%. The results show that the electron densities obtained from the linear attenuation coefficient (reDLAC) for the tissue equivalent material used in the phantom is a better option for validation.Conclusions:This validated phantom has numerous advantages over the origin type of RANDO phantom. Therefore, using it in TBI and TSET dosimetry is recommendable.
The political economy of (de)regulation: theory and evidence from the US electricity industry
Authors: Guerriero Carmine 
Published: October 2019

AbstractThe choice of whether to regulate firms or to allow them to compete is key. If demand is sufficiently inelastic, competition entails narrower allocative inefficiencies, but also smaller expected profits, and thus weaker incentives to invest in cost reduction. Hence, deregulation should be found where cost reduction is less socially relevant and consumers are more politically powerful, and it should produce lower expected costs only when investment is not sufficiently effective. These predictions hold true under several alternative assumptions and are consistent with data on the deregulation initiatives implemented in 43 US state electricity markets between 1981 and 1999 and on the operating costs of the plants that served these markets. Crucially, these empirical results help rationalize the slowdown of the deregulation wave and are robust to considering the other determinants of deregulation emphasized by the extant literature, i.e. costly long-term wholesale contracts and excessive capacity accumulation.
Support for Refugee Integration in West and East	Germany
Authors: Hellmann Jens H. , Forthmann Boris , Knausenberger Judith , Hellmann Deborah F. , Rees Jonas H. , Gansel Eva , Back Mitja D. , Echterhoff Gerald 
Published: October 2019

Abstract. Prior research has reported less favorable attitudes toward and more violent crimes against ethnic out-group members in East (vs. West) Germany. We conducted two pre-registered lost letter studies in West versus East German cities (Study 1, N = 400) and in West versus East German rural areas (Study 2, N = 400). To investigate supportive behavior regarding refugee integration, we manipulated the addressee (refugee-integration vs. immigration-stop projects). Contrary to predictions, letter return rates did not differ between West and East Germany. Across western and eastern German regions, return rates were higher for the refugee-integration project in urban areas while no differences emerged in rural areas. A pooled analysis found greater support for the refugee-integration (vs. immigration-stop) project.
Targeting chemoresistant colorectal cancer via systemic administration of a BMP7 variant
Authors: Veschi Veronica , Mangiapane Laura R. , Nicotra Annalisa , Di Franco Simone , Scavo Emanuela , Apuzzo Tiziana , Sardina Davide S. , Fiori Micol , Benfante Antonina , Colorito Maria L. , Cocorullo Gianfranco , Giuliante Felice , Cipolla Calogero , Pistone Giuseppe , Bongiorno Maria Rita , Rizzo Aroldo , Tate Courtney M. , Wu Xiaohua , Rowlinson Scott , Stancato Louis F. , Todaro Matilde , De Maria Ruggero , Stassi Giorgio 
Published: October 2019

Abstract
Despite intense research and clinical efforts, patients affected by advanced colorectal cancer (CRC) have still a poor prognosis. The discovery of colorectal (CR) cancer stem cell (CSC) as the cell compartment responsible for tumor initiation and propagation may provide new opportunities for the development of new therapeutic strategies. Given the reduced sensitivity of CR-CSCs to chemotherapy and the ability of bone morphogenetic proteins (BMP) to promote colonic stem cell differentiation, we aimed to investigate whether an enhanced variant of BMP7 (BMP7v) could sensitize to chemotherapy-resistant CRC cells and tumors. Thirty-five primary human cultures enriched in CR-CSCs, including four from chemoresistant metastatic lesions, were used for in vitro studies and to generate CR-CSC-based mouse avatars to evaluate tumor growth and progression upon treatment with BMP7v alone or in combination with standard therapy or PI3K inhibitors. BMP7v treatment promotes CR-CSC differentiation and recapitulates the cell differentiation-related gene expression profile by suppressing Wnt pathway activity and reducing mesenchymal traits and survival of CR-CSCs. Moreover, in CR-CSC-based mouse avatars, BMP7v exerts an antiangiogenic effect and sensitizes tumor cells to standard chemotherapy regardless of the mutational, MSI, and CMS profiles. Of note, tumor harboring PIK3CA mutations were affected to a lower extent by the combination of BMP7v and chemotherapy. However, the addition of a PI3K inhibitor to the BMP7v-based combination potentiates PIK3CA-mutant tumor drug response and reduces the metastatic lesion size. These data suggest that BMP7v treatment may represent a useful antiangiogenic and prodifferentiation agent, which renders CSCs sensitive to both standard and targeted therapies.
A combined miRNA–piRNA signature to detect Alzheimer’s disease
Authors: Jain Gaurav , Stuendl Anne , Rao Pooja , Berulava Tea , Pena Centeno Tonatiuh , Kaurani Lalit , Burkhardt Susanne , Delalle Ivana , Kornhuber Johannes , Hüll Michael , Maier Wolfgang , Peters Oliver , Esselmann Hermann , Schulte Claudia , Deuschle Christian , Synofzik Mathis , Wiltfang Jens , Mollenhauer Brit , Maetzler Walter , Schneider Anja , Fischer Andre 
Published: October 2019

AbstractAlzheimer’s disease (AD) is the most common neurodegenerative disorder causing huge emotional and economic burden to our societies. An effective therapy has not been implicated yet, which is in part also due to the fact that pathological changes occur years before clinical symptoms manifest. Thus, there is a great need for the development of a translatable biomarker. Recent evidence highlights microRNAs as candidate biomarkers. In this study, we use next-generation sequencing to study the small noncoding RNAome (sncRNAome) in exosomes derived from human cerebrospinal fluid (CSF). We show that the sncRNAome from CSF-derived exosomes is dominated not only by microRNAs (miRNAs) but also by PIWI-interacting RNAs (piRNAs). We define a combined signature consisting of three miRNAs and three piRNAs that are suitable to detect AD with an AUC of 0.83 in a replication cohort and furthermore predict the conversion of mild–cognitive impaired (MCI) patients to AD dementia with an AUC of 0.86 for the piRNA signature. When combining the smallRNA signature with pTau and Aβ 42/40 ratio the AUC reaches 0.98. Our study reports a novel exosomal small noncoding RNA signature to detect AD pathology and provides the first evidence that in addition to miRNAs, piRNAs should also be considered as a candidate biomarker for AD.
Impaired adult neurogenesis is an early event in Alzheimer’s disease neurodegeneration, mediated by intracellular Aβ oligomers
Authors: Scopa Chiara , Marrocco Francesco , Latina Valentina , Ruggeri Federica , Corvaglia Valerio , La Regina Federico , Ammassari-Teule Martine , Middei Silvia , Amadoro Giuseppina , Meli Giovanni , Scardigli Raffaella , Cattaneo Antonino 
Published: October 2019

AbstractAlterations of adult neurogenesis have been reported in several Alzheimer's disease (AD) animal models and human brains, while defects in this process at presymptomatic/early stages of AD have not been explored yet. To address this, we investigated potential neurogenesis defects in Tg2576 transgenic mice at 1.5 months of age, a prodromal asymptomatic age in terms of Aβ accumulation and neurodegeneration. We observe that Tg2576 resident and SVZ-derived adult neural stem cells (aNSCs) proliferate significantly less. Further, they fail to terminally differentiate into mature neurons due to pathological, tau-mediated, and microtubule hyperstabilization. Olfactory bulb neurogenesis is also strongly reduced, confirming the neurogenic defect in vivo. We find that this phenotype depends on the formation and accumulation of intracellular A-beta oligomers (AβOs) in aNSCs. Indeed, impaired neurogenesis of Tg2576 progenitors is remarkably rescued both in vitro and in vivo by the expression of a conformation-specific anti-AβOs intrabody (scFvA13-KDEL), which selectively interferes with the intracellular generation of AβOs in the endoplasmic reticulum (ER). Altogether, our results demonstrate that SVZ neurogenesis is impaired already at a presymptomatic stage of AD and is caused by endogenously generated intracellular AβOs in the ER of aNSCs. From a translational point of view, impaired SVZ neurogenesis may represent a novel biomarker for AD early diagnosis, in association to other biomarkers. Further, this study validates intracellular Aβ oligomers as a promising therapeutic target and prospects anti-AβOs scFvA13-KDEL intrabody as an effective tool for AD treatment.
Intermittent compressive force promotes osteogenic differentiation in human periodontal ligament cells by regulating the transforming growth factor-β pathway
Authors: Manokawinchoke Jeeranan , Pavasant Prasit , Sawangmake Chenphop , Limjeerajarus Nuttapol , Limjeerajarus Chalida N. , Egusa Hiroshi , Osathanon Thanaphum 
Published: October 2019

AbstractMechanical force regulates periodontal ligament cell (PDL) behavior. However, different force types lead to distinct PDL responses. Here, we report that pretreatment with an intermittent compressive force (ICF), but not a continuous compressive force (CCF), promoted human PDL (hPDL) osteogenic differentiation as determined by osteogenic marker gene expression and mineral deposition in vitro. ICF-induced osterix (OSX) expression was inhibited by cycloheximide and monensin. Although CCF and ICF significantly increased extracellular adenosine triphosphate (ATP) levels, pretreatment with exogenous ATP did not affect hPDL osteogenic differentiation. Gene-expression profiling of hPDLs subjected to CCF or ICF revealed that extracellular matrix (ECM)-receptor interaction, focal adhesion, and transforming growth factor beta (TGF-β) signaling pathway genes were commonly upregulated, while calcium signaling pathway genes were downregulated in both CCF- and ICF-treated hPDLs. The TGFB1 mRNA level was significantly increased, while those of TGFB2 and TGFB3 were decreased by ICF treatment. In contrast, CCF did not modify TGFB1 expression. Inhibiting TGF-β receptor type I or adding a TGF-β1 neutralizing antibody attenuated the ICF-induced OSX expression. Exogenous TGF-β1 pretreatment promoted hPDL osteogenic marker gene expression and mineral deposition. Additionally, pretreatment with ICF in the presence of TGF-β receptor type I inhibitor attenuated the ICF-induced mineralization. In conclusion, this study reveals the effects of ICF on osteogenic differentiation in hPDLs and implicates TGF-β signaling as one of its regulatory mechanisms.
Genome sequences of horticultural plants: past, present, and future
Authors: Chen Fei , Song Yunfeng , Li Xiaojiang , Chen Junhao , Mo Lan , Zhang Xingtan , Lin Zhenguo , Zhang Liangsheng 
Published: October 2019

Abstract
Horticultural plants play various and critical roles for humans by providing fruits, vegetables, materials for beverages, and herbal medicines and by acting as ornamentals. They have also shaped human art, culture, and environments and thereby have influenced the lifestyles of humans. With the advent of sequencing technologies, there has been a dramatic increase in the number of sequenced genomes of horticultural plant species in the past decade. The genomes of horticultural plants are highly diverse and complex, often with a high degree of heterozygosity and a high ploidy due to their long and complex history of evolution and domestication. Here we summarize the advances in the genome sequencing of horticultural plants, the reconstruction of pan-genomes, and the development of horticultural genome databases. We also discuss past, present, and future studies related to genome sequencing, data storage, data quality, data sharing, and data visualization to provide practical guidance for genomic studies of horticultural plants. Finally, we propose a horticultural plant genome project as well as the roadmap and technical details toward three goals of the project.
Melatonin enhances salt tolerance by promoting MYB108A-mediated ethylene biosynthesis in grapevines
Authors: Xu Lili , Xiang Guangqing , Sun Qinghua , Ni Yong , Jin Zhongxin , Gao Shiwei , Yao Yuxin 
Published: October 2019

Abstract
The signal molecules melatonin and ethylene play key roles in abiotic stress tolerance. The interplay between melatonin and ethylene in regulating salt tolerance and the underlying molecular mechanism of this interplay remain unclear. Here, we found that both melatonin and 1-aminocyclopropane-1-carboxylic acid (ACC, a precursor of ethylene) enhanced the tolerance of grapevine to NaCl; additionally, ethylene participated in melatonin-induced salt tolerance. Further experiments indicated that exogenous treatment and endogenous induction of melatonin increased the ACC content and ethylene production in grapevine and tobacco plants, respectively. The expression of MYB108A and ACS1, which function as a transcription factor and a key gene involved in ethylene production, respectively, was strongly induced by melatonin treatment. Additionally, MYB108A directly bound to the promoter of ACS1 and activated its transcription. MYB108A expression promoted ACC synthesis and ethylene production by activating ACS1 expression in response to melatonin treatment. The suppression of MYB108A expression partially limited the effect of melatonin on the induction of ethylene production and reduced melatonin-induced salt tolerance. Collectively, melatonin promotes ethylene biosynthesis and salt tolerance through the regulation of ACS1 by MYB108A.
Evolution and regulation of nitrogen flux through compartmentalized metabolic networks in a marine diatom
Authors: Smith Sarah R. , Dupont Chris L. , McCarthy James K. , Broddrick Jared T. , Oborník Miroslav , Horák Aleš , Füssy Zoltán , Cihlář Jaromír , Kleessen Sabrina , Zheng Hong , McCrow John P. , Hixson Kim K. , Araújo Wagner L. , Nunes-Nesi Adriano , Fernie Alisdair , Nikoloski Zoran , Palsson Bernhard O. , Allen Andrew E. 
Published: October 2019

AbstractDiatoms outcompete other phytoplankton for nitrate, yet little is known about the mechanisms underpinning this ability. Genomes and genome-enabled studies have shown that diatoms possess unique features of nitrogen metabolism however, the implications for nutrient utilization and growth are poorly understood. Using a combination of transcriptomics, proteomics, metabolomics, fluxomics, and flux balance analysis to examine short-term shifts in nitrogen utilization in the model pennate diatom in Phaeodactylum tricornutum, we obtained a systems-level understanding of assimilation and intracellular distribution of nitrogen. Chloroplasts and mitochondria are energetically integrated at the critical intersection of carbon and nitrogen metabolism in diatoms. Pathways involved in this integration are organelle-localized GS-GOGAT cycles, aspartate and alanine systems for amino moiety exchange, and a split-organelle arginine biosynthesis pathway that clarifies the role of the diatom urea cycle. This unique configuration allows diatoms to efficiently adjust to changing nitrogen status, conferring an ecological advantage over other phytoplankton taxa.
Long-term memory is formed immediately without the need for protein synthesis-dependent consolidation in Drosophila
Authors: Zhao Bohan , Sun Jiameng , Zhang Xuchen , Mo Han , Niu Yijun , Li Qian , Wang Lianzhang , Zhong Yi 
Published: October 2019

AbstractIt is believed that long-term memory (LTM) cannot be formed immediately because it must go through a protein synthesis-dependent consolidation process. However, the current study uses Drosophila aversive olfactory conditioning to show that such processes are dispensable for context-dependent LTM (cLTM). Single-trial conditioning yields cLTM that is formed immediately in a protein-synthesis independent manner and is sustained over 14 days without decay. Unlike retrieval of traditional LTM, which requires only the conditioned odour and is mediated by mushroom-body neurons, cLTM recall requires both the conditioned odour and reinstatement of the training-environmental context. It is mediated through lateral-horn neurons that connect to multiple sensory brain regions. The cLTM cannot be retrieved if synaptic transmission from any one of these centres is blocked, with effects similar to those of altered encoding context during retrieval. The present study provides strong evidence that long-term memory can be formed easily without the need for consolidation.