bluuebunny/crossref_metadata_2025_split huggingface dataset:
Alcohol‐mediated calcium signals dysregulate pro‐survival Snai2/PUMA/Bcl2 networks to promote p53‐mediated apoptosis in avian neural crest progenitors
Authors: Flentke George R. , Baulch Joshua W. , Berres Mark E. , Garic Ana , Smith Susan M. 
Published: April 2019

AbstractBackgroundPrenatal alcohol exposure causes distinctive craniofacial anomalies that arise, in part, from the apoptotic elimination of neural crest (NC) progenitors that form the face. This vulnerability of NC to alcohol is puzzling as they normally express the transcriptional repressor Snail1/2 (in chick Snai2), which suppresses apoptosis and promotes their migration. Here, we investigate alcohol's impact upon Snai2 function.MethodsChick cranial NC cells were treated with acute alcohol (52 mM, 2 hr). We evaluated NC migration, gene expression, proliferation, and apoptosis thereafter.ResultsTransient alcohol exposure induced Snai2 (191% ± 23%; p = .003) and stimulated NC migration (p = .0092). An alcohol‐induced calcium transient mediated this Snai2 induction, and BAPTA‐AM blocked whereas ionomycin mimicked these pro‐migratory effects. Alcohol suppressed CyclinD1 protein content (59.1 ± 12%, p = .007) and NC proliferation (19.7 ± 5.8%, p < .001), but these Snai2‐enriched cells still apoptosed in response to alcohol. This was explained because alcohol induced p53 (198 ± 29%, p = .023), and the p53 antagonist pifithrin‐α prevented their apoptosis. Moreover, alcohol counteracted Snai2's pro‐survival signals, and Bcl2 was repressed (68.5 ± 6.0% of controls, p = .016) and PUMA was not induced, while ATM (1.32‐fold, p = .01) and PTEN (1.30‐fold, p = .028) were elevated.ConclusionsAlcohol's calcium transient uncouples the Snai2/p53 regulatory loop that normally prevents apoptosis during EMT. This represents a novel pathway in alcohol's neurotoxicity, and complements demonstrations that alcohol suppresses PUMA in mouse NC. We propose that the NCs migratory behavior, and their requirement for Snai2/p53 co‐expression, makes them vulnerable to stressors that dysregulate Snai2/p53 interactions, such as alcohol.
Saturation mutagenesis and self‐inducible expression of trehalose synthase in Bacillus subtilis
Authors: Liu Hongling , Wang Xihui , Yang Shaojie , Wang Ruiming , Wang Tengfei 
Published: April 2019

AbstractTrehalose is a nonreducing disaccharide synthesized by trehalose synthase (TreS), which catalyzes the reversible interconversion of maltose and trehalose. We aimed to enhance the catalytic conversion of maltose to trehalose by saturation mutagenesis, and constructed a self‐inducible TreS expression system by generating a robust Bacillus subtilis recombinant. We found that the conversion yield and enzymatic activity of TreS was enhanced by saturation mutations, especially by the combination of V407M and K490L mutations. At the same time, these saturation mutations were contributing to reducing by‐products in the reaction. Compared to WT TreS, the conversion yield of maltose to trehalose was increased by 11.9%, and the kcat/Km toward trehalose was 1.33 times higher in the reaction catalyzed by treSV407M‐K490L. treSV407M‐K490L expression was further observed in the recombinant B. subtilis W800N(ΔσF) under the influence of PsrfA, Pcry3Aa, and PsrfA‐cry3Aa promoters without an inducer. It was shown that PsrfA‐cry3Aa was evidently a stronger promoter for treSV407M‐K490L expression, with the intracellular enzymatic activity of recombinant treSV407M‐K490L being over 5,800 U/g at 35 hr in TB medium. These results suggested the combination of two mutations, V407M and K490L, was conducive for the production of trehalose. In addition, the self‐inducible TreSV407M/K490L mutant in the B. subtilis host provides a low‐cost choice for the industrial production of endotoxin‐free trehalose with high yields.
Percutaneous axillary artery approach for ductal stenting in critical right ventricular outflow tract lesions in the neonatal period
Authors: Breatnach Colm R. , Aggarwal Varun , Al‐Alawi Khalid , McMahon Colin J. , Franklin Orla , Prendiville Terence , Oslizlok Paul , Walsh Kevin , Qureshi Athar M. , Kenny Damien 
Published: April 2019

AbstractObjectivesWe aimed to assess the experience using a percutaneous axillary artery approach for insertion of arterial ductal stents in patients with critical right ventricular outflow tract lesions at two tertiary pediatric cardiology centers.BackgroundPatent ductus arteriosus stenting is an accepted palliative alternative to BT shunts for neonates with critical right heart lesions. Access to tortuous ductus' may be challenging via the femoral artery, whereas the carotid artery presents a low risk of stroke. Recently, the axillary artery has been utilized for access in these patients.MethodsWe performed a retrospective review of neonates who underwent stent placement or angioplasty using percutaneous axillary artery approach at two tertiary care centers from October 2016 to November 2018. Medical records were reviewed to ascertain demographic, clinical, and outcome data.ResultsAxillary artery access was performed in 20 patients (16 primary ductal stents and 4 re‐interventions) at a median (IQR) procedural weight of 3.4 (3–3.9) kg. Median (IQR) procedural time was 110 (75–150) min. The median (IQR) ICU stay and intubation times were 14 (0–94) hr and 5 (0–40) hr, respectively. There were three access‐related vascular complications which were managed conservatively with no long‐term effects. Two patients subsequently died due to non‐procedure related causes.ConclusionsDuctal stenting via a percutaneous axillary artery approach is a viable option in neonates with critical right ventricular outflow tract lesions. This approach provides an additional access site for PDA stenting which may be utilized in patients with vertical duct morphology.
Engineered Recombinant Proteins for Aqueous Ultrasonic Exfoliation and Dispersion of Biofunctionalized 2D Materials
Authors: Li Bing , Wang Tiankuo , Wang Xin , Wu Xin , Wang Chenyu , Miao Feng , Qin Meng , Wang Wei , Cao Yi 
Published: April 2019

AbstractInvited for the cover of this issue are the groups of Wei Wang and Yi Cao at the Nanjing University. The image depicts engineered recombinant proteins binding specifically to graphene layers, allowing for their efficient exfoliation and dispersion. Read the full text of the article at 10.1002/chem.201900716.
Enantioselective Catalytic [4+1]‐Cyclization of ortho‐Hydroxy‐para‐Quinone Methides with Allenoates
Authors: Zielke Katharina , Kováč Ondřej , Winter Michael , Pospíšil Jiří , Waser Mario 
Published: April 2019

AbstractThe first highly asymmetric catalytic synthesis of densely functionalized dihydrobenzofurans is reported, which starts from ortho‐hydroxy‐containing para‐quinone methides. The reaction relies on an unprecedented formal [4+1]‐annulation of these quinone methides with allenoates in the presence of a commercially available chiral phosphine catalyst. The chiral dihydrobenzofurans were obtained as single diastereomers in yields up to 90 % and with enantiomeric ratios up to 95:5.
The impact of environmental, social, and governance performance on stock prices: Evidence from the banking industry
Authors: Miralles‐Quirós María Mar , Miralles‐Quirós José Luis , Redondo‐Hernández Jesús 
Published: April 2019

AbstractAfter the global financial crisis, commercial banks have increased their social responsibility activities with the aim of reinforcing the credibility and trust that their stakeholders have in them. However, prior research about the value relevance for their financial stakeholders of these sustainable practices is scarce. In this context, the aim of this research is to examine whether environmental, social, and governance (ESG) performance of commercial banks listed on 20 different stock markets provides relevant information and has a significant impact on stock prices over the 2002–2015 period. Our overall results reveal that stock market investors value the three ESG pillars in a different manner. We also observe that the value relevance of ESG performance is significantly higher for banks from common law countries and after the global financial crisis. These findings could have several implications for internal and external stakeholders such as managers, investors, and market regulators.
Relationships of biological and environmental factors to cognition of preterm infants in the toddler and preschool periods
Authors: Ross Gail S. , Perlman Jeffrey M. 
Published: April 2019

AbstractOne hundred and nine preterm infants were studied to examine the relative effects of biologic/neurologic factors (length of hospital stay, 18 month cognitive status) and environment (socioeconomic status) on cognition in the toddler (18 months) and preschool periods (3 years). Length of hospital stay was significantly related to toddler cognitive outcome, and less so to preschool outcome. Socioeconomic status predicted only preschool cognitive outcome and not toddler outcome. Cognitive status at 18 months significantly predicted 3 year outcome, and there was relatively little change between those periods. Together, cognitive status at 18 months and socioeconomic status significantly predicted preschool IQ, accounting for 34% of the variance. Results showed that perinatal biologic risks became less salient while socioeconomic status became increasingly important at the preschool period. Relative lack of change in cognitive status indicated the importance of early cognitive evaluation in preterm infants.
Budget impact of intravenous ferric carboxymaltose in patients with chronic heart failure and iron deficiency in France
Authors: Bourguignon Sandrine , Faller Mathilde , Champs François‐Olivier , Moutier Hélène , Levesque Karine , Caranhac Gilbert , Cohen‐Solal Alain 
Published: April 2019

AbstractAimsThis analysis aims to evaluate the budget impact of intravenous iron therapy with ferric carboxymaltose for patients with systolic chronic heart failure and iron deficiency, from the perspective of the French public health insurance.Methods and resultsA budget impact model was adapted to forecast the budget impact over 5 years, according to two scenarios: one where patients receive ferric carboxymaltose according to market share forecast and another where patients are not treated for iron deficiency. Clinical data were extrapolated from pooled data from four randomized controlled trials. The time horizon was extended to 5 years by applying transition probabilities estimated from the CONFIRM‐HF trial. Epidemiological parameters for France were derived from the literature. Cost parameters were derived from national available databases.In the base case analysis, the modelled 5 year cost difference between the scenarios with ferric carboxymaltose vs. no iron deficiency treatment in a population of 189 334 prevalent and incident patients led to €0.8m savings. The cumulative savings resulted from a reduction in the hospitalization costs associated with worsening heart failure (€−35.8m) as well as a reduction in the follow‐up costs (€−2.9m). These cost savings outweighed the costs of ferric carboxymaltose treatment (€37.7m). Sensitivity analyses showed that the budget impact varied from €−34m to €+146m. Parameters with the most impact on the budget were the hospitalization rate for patients not treated for iron deficiency, the number of ambulatory sessions needed, the absence of hospitalization cost differentiation between New York Heart Association classes, and administration settings costs.ConclusionsIron deficiency treatment with ferric carboxymaltose in systolic chronic heart failure patients results in an improvement of New York Heart Association class and thereby increases the well‐being of the patients, while providing an overall cost saving for the French national health insurance.
Strain‐encoded magnetic resonance: a method for the assessment of myocardial deformation
Authors: Korosoglou Grigorios , Giusca Sorin , Hofmann Nina P. , Patel Amit R. , Lapinskas Tomas , Pieske Burkert , Steen Henning , Katus Hugo A. , Kelle Sebastian 
Published: April 2019

AbstractThis study aims to assess the usefulness of strain‐encoded magnetic resonance (SENC) for the quantification of myocardial deformation (‘strain’) in healthy volunteers and for the diagnostic workup of patients with different cardiovascular pathologies. SENC was initially described in the year 2001. Since then, the SENC sequence has undergone several technical developments, aiming at the detection of strain during single‐heartbeat acquisitions (fast‐SENC). Experimental and clinical studies that used SENC and fast‐SENC or compared SENC with conventional cine or tagged magnetic resonance in phantoms, animals, healthy volunteers, or patients were systematically searched for in PubMed. Using ‘strain‐encoded magnetic resonance and SENC’ as keywords, three phantom and three animal studies were identified, along with 27 further clinical studies, involving 185 healthy subjects and 904 patients. SENC (i) enabled reproducible assessment of myocardial deformation in vitro, in animals and in healthy volunteers, (ii) showed high reproducibility and substantially lower time spent compared with conventional tagging, (iii) exhibited incremental value to standard cine imaging for the detection of inducible ischaemia and for the risk stratification of patients with ischaemic heart disease, and (iv) enabled the diagnostic classification of patients with transplant vasculopathy, cardiomyopathies, pulmonary hypertension, and diabetic heart disease. SENC has the potential to detect a wide range of myocardial diseases early, accurately, and without the need of contrast agent injection, possibly enabling the initiation of specific cardiac therapies during earlier disease stages. Its one‐heartbeat acquisition mode during free breathing results in shorter cardiovascular magnetic resonance protocols, making its implementation in the clinical realm promising.
Glassy Carbon Electrode Modified via Molybdenum Disulfide Decorated Multiwalled Carbon Nanotubes for Sensitive Voltammetric Detection of Aristolochic Acids
Authors: Wang Ying , Mamat Xamxikamar , Li Yongtao , Hu Xun , Wang Ping , Dong Yemin , Hu Guangzhi 
Published: April 2019

AbstractIn this study, a molybdenum disulfide/multiwalled carbon nanotubes (MoS2@MWCNTs) nanocomposite was synthesized by employing a simple hydrothermal method. The flower‐like structure of the MoS2@MWCNTs was characterized via scanning electron microscopy (SEM) and transmission electron microscopy (TEM), and the load of crystalline MoS2 was verified via X‐ray diffraction (XRD) and energy‐dispersive spectroscopy (EDS). The as‐prepared MoS2@MWCNTs nanocomposite was used to modify glassy carbon electrode (GCE) as an electrochemical sensor for detecting aristolochic acids (AAs). With the optimized parameters, the proposed electrochemical sensor exhibited good sensitivity and a broad linear concentration range for detecting AAs from 0.2 to 10 μmol/L and 10 to 100 μmol/L, with the sensitivity of −3.10 μA/(μmol/L) and −0.91 μA/(μmol/L), respectively. The detection limit was also calculated as 0.06 μmol/L (S/N=3) based on the low background signal. Furthermore, the modified electrochemical sensor exhibited good selectivity, repeatability, reproducibility, and stability, thus showing potential application for detecting AA in chinese herbs with good mean recovery and accuracy. In other words, the MoS2@MWCNTs/GCE can be used as an excellent platform to detect AAs.
Reversing current rectification to improve DNA‐sensing sensitivity in conical nanopores
Authors: Cai Xiu‐Hong , Cao Shuo‐Hui , Cai Sheng‐Lin , Wu Yuan‐Yi , Ajmal Muhammad , Li Yao‐Qun 
Published: April 2019

AbstractHerein, we report the ultrasensitive DNA detection through designing an elegant nanopore biosensor as the first case to realize the reversal of current rectification direction for sensing. Attributed to the unique asymmetric structure, the glass conical nanopore exhibits the sensitive response to the surface charge, which can be facilely monitored by ion current rectification curves. In our design, an enzymatic cleavage reaction was employed to alter the surface charge of the nanopore for DNA sensing. The measured ion current rectification was strongly responsive to DNA concentrations, even reaching to the reversed status from the negative ratio (−6.5) to the positive ratio (+16.1). The detectable concentration for DNA was as low as 0.1 fM. This is an ultrasensitive and label‐free DNA sensing approach, based on the rectification direction‐reversed amplification in a single glass conical nanopore.
Self‐centering friction spring dampers for seismic resilience
Authors: Wang Wei , Fang Cheng , Zhao Yashuo , Sause Richard , Hu Shuling , Ricles James 
Published: April 2019

SummaryThis paper presents a comprehensive study on self‐centering dampers equipped with friction springs. The basic mechanical behavior of individual friction springs is first understood via analytical descriptions that are verified by a preliminary experimental study. The working principle, fabrication process, and mechanical performance of the proposed dampers are subsequently described, where the design details related to seismic applications are highlighted. This is followed by physical tests on three damper specimens, where the influence of the treatment of the taper surfaces of the friction springs on the overall damper behavior is examined, and the reliability of the dampers under repeated rounds of cyclic loading is evaluated. The damper specimens show reliable flag‐shaped load‐deformation hysteretic curves with excellent self‐centering capability. Satisfactory energy dissipation with an equivalent viscous damping (EVD) of up to 20% is shown, and the EVD is stabilized throughout the entire loading process. The dampers are capable of withstanding multiple rounds of cyclic loading with stable hysteretic behavior, indicating that they are fully reusable after multiple strong earthquakes. A larger friction coefficient on the taper surfaces leads to increased yield load and energy dissipation. Following the experimental study, some practical design recommendations are provided. Based on the available analytical model, an additional parametric study is performed to highlight the influences of precompression and friction condition of the friction springs on the damper behavior. Finally, the seismic performance of a building which adopts the friction spring dampers is evaluated via numerical simulation.
Anxiety and depressive symptoms are related to core symptoms, general health outcome, and medical comorbidities in eating disorders
Authors: Martín Josune , Arostegui Inmaculada , Loroño Ane , Padierna Angel , Najera‐Zuloaga Josu , Quintana José M. 
Published: April 2019

AbstractObjectiveThe goal of this study is to identify potential factors that have a significant effect on anxiety and depression of patients with eating disorders (ED) using the beta‐binomial regression (BBR) approach on a broad sample of patients.MethodThis cross‐sectional study involved 520 ED patients. The effect of sociodemographics, core symptoms, general health outcomes, and medical comorbidities in anxiety and depression were analysed jointly using the beta‐binomial mixed‐effects model.ResultsTwo hundred fifty‐five (49.4%) patients had anorexia nervosa, 173 (33.3%) patients had bulimia nervosa, and 92 (17.7%) had ED not otherwise specified. A high level of anxiety was associated, among other variables, with having a restrictive ED subtype as compared with purgative and binge ED subtypes (β = −0.2, p < 0.001, OR = 0.82 and β = −0.16, p < 0.03, OR = 0.85, respectively), with having a high level of ED symptomatology or with living not alone (β = −0.23, p = 0.002, OR = 0.80). A high level of depression was associated, among other variables, with older age (β = 0.74, p < 0.001, OR = 2.1).ConclusionsThe results of our study suggest that depressive and anxiety symptoms are related to ED symptoms, health status, ED subtype, medical comorbitidy, and educational level. In addition, another interesting finding is the inverse association between symptomatology of anxiety and living alone. Finally, BBR may be a useful approach in interpreting patient‐reported outcome as odds.
From books to bytes: A new data rescue tool
Authors: Slonosky Victoria , Sieber Renée , Burr Gordon , Podolsky Lori , Smith Robert , Bartlett Madeleine , Park Eun , Cullen Jeremy , Fabry Frédéric 
Published: April 2019

AbstractHistorical data provides observational information crucial to our understanding of the evolution of geophysical processes. However, there is a gap between predigital age observations, which are typically handwritten, and data that is discoverable and analysable. The data rescue protocols here address this gap, covering the information lifecycle from handwritten register pages to transcription‐ready content, describing the historical data, the database design for the data rescue, and the development of an application design to transcribe the meteorological information directly from an image file to the database. The preparatory steps necessary to organize, curate, image, and structure the meteorological information, prior to transcribing the historical data, are outlined here in an integrated methodology. The initial organization, the development of an image file nomenclature to link the rescued data to the original source, and the description of a metadata schema to optimize the transcription application are all vital to the process of ensuring traceability and transparency in the data rescue process. Taken together, these steps describe best practices guidelines for similar projects. Although we designed the methodology and application to be used in any data rescue context, our particular concern was to accommodate the needs of citizen scientists. We thus focused on making our application easily maintained, flexible, direct to database, clear, and simple to use.Open Practices

This article has earned an Open Data badge for making publicly available the digitally‐shareable data necessary to reproduce the reported results. The data is available at https://citsci.geog.mcgill.ca. Learn more about the Open Practices badges from the Center for Open Science: https://osf.io/tvyxz/wiki.
Agathisflavone: Botanical sources, therapeutic promises, and molecular docking study
Authors: Islam Muhammad Torequl , Zihad S. M. Neamul Kabir , Rahman Md. Shamim , Sifat Nazifa , Khan Md. Roich , Uddin Shaikh Jamal , Rouf Razina 
Published: April 2019

AbstractIn this article, we have summarized the biological sources and pharmacological activities of agathisflavone along with molecular docking studies to correlate the interaction of this biflavonoid and biomacromolecules involving in its biological effects observed in database‐oriented scientific reports. For this, an up‐to‐date (from 1991 to October 2018) search was done on the databases such as PubMed, Science Direct, Web of Science, Scopus, The American Chemical Society, Clinicaltrials.gov, and Google Scholar databases. The findings suggest that agathisflavone possesses antioxidant, anti‐inflammatory, antiviral, antiparasitic, cytotoxic, neuroprotective, and hepatoprotective activities. An in silico study of agathisflavone against 17 essential proteins/enzymes revealed that inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)‐2 are the most efficient enzymes for the interaction and binding of this biflavonoid for its anti‐inflammatory activity. In conclusion, agathisflavone may be one of the promising plant‐derived lead compounds in the treatment of oxidative stress, inflammatory diseases, microbial infection, hepatic and neurological diseases and disorders, and cancer. © 2019 IUBMB Life, 71(9):1192–1200, 2019