bluuebunny/crossref_metadata_2025_split huggingface dataset:
β-cyclocitral synergizes the response of adult Drosophila suzukii (Diptera: Drosophilidae) to fruit juices and isoamyl acetate in a sex-dependent manner
Authors: Piñero Jaime C. , Barrett Bruce A. , Bolton Leland Grant , Follett Peter A. 
Published: July 2019

AbstractSemiochemicals play a pivotal role in the location, evaluation, and utilization of hosts by herbivorous insects. Mixtures of host plant-derived compounds are often required to elicit appropriate levels of response to olfactory stimuli. In multiple-choice bioassays, we characterized the response of adult Drosophila suzukii to foliage- and fruit-based synthetic compounds tested alone and in association with grape and tart cherry juices, and assessed whether synergistic interactions among olfactory stimuli are involved in the olfactory-driven behavior of D. suzukii. Our results established (1) significant attraction of females (but not males) to β-cyclocitral and isoamyl acetate when tested singly, (2) the presence of a synergistic interaction between β-cyclocitral and cherry juice only for females, and (3) the presence of a synergistic interaction between β-cyclocitral and isoamyl acetate but only in the case of males. Our findings increase our understanding of male and female D. suzukii olfactory responses to synthetic compounds and fruit juices as sources of attractants. Combinations of foliage- and fruit-based compounds may be needed to increase SWD attraction.
Systems biology and network pharmacology of frailty reveal novel epigenetic targets and mechanisms
Authors: Gomez-Verjan J. C. , Ramírez-Aldana R. , Pérez-Zepeda M. U. , Quiroz-Baez R. , Luna-López A. , Gutierrez Robledo L. M. 
Published: July 2019

AbstractFrailty is an age-associated condition, characterized by an inappropriate response to stress that results in a higher frequency of adverse outcomes (e.g., mortality, institutionalization and disability). Some light has been shed over its genetic background, but this is still a matter of debate. In the present study, we used network biology to analyze the interactome of frailty-related genes at different levels to relate them with pathways, clinical deficits and drugs with potential therapeutic implications. Significant pathways involved in frailty: apoptosis, proteolysis, muscle proliferation, and inflammation; genes as FN1, APP, CREBBP, EGFR playing a role as hubs and bottlenecks in the interactome network and epigenetic factors as HIST1H3 cluster and miR200 family were also involved. When connecting clinical deficits and genes, we identified five clusters that give insights into the biology of frailty: cancer, glucocorticoid receptor, TNF-α, myostatin, angiotensin converter enzyme, ApoE, interleukine-12 and −18. Finally, when performing network pharmacology analysis of the target nodes, some compounds were identified as potentially therapeutic (e.g., epigallocatechin gallate and antirheumatic agents); while some other substances appeared to be toxicants that may be involved in the development of this condition.
Investigating fouling at the pore-scale using a microfluidic membrane mimic filtration system
Authors: Debnath Nandini , Kumar Aloke , Thundat Thomas , Sadrzadeh Mohtada 
Published: July 2019

AbstractThe work investigates fouling in a microfluidic membrane mimic (MMM) filtration system for foulants such as polystyrene particles and large polymeric molecules. Our MMM device consists of a staggered arrangement of pillars which enables real-time visualization and analysis of pore-scale phenomena. Different fouling scenarios are investigated by conducting constant-pressure experiments. Fouling experiments are performed with three different types of foulants: polystyrene particle solution (colloidal fouling), polyacrylamide polymer solution (organic fouling) and a mixture of these two solutions (combined fouling). Four major categories of microscopic fouling are observed: cake filtration (upstream), pore blocking (inside the pores), colloidal aggregation (downstream) and colloidal streamer fouling (downstream). Our microfluidic experiments show that downstream colloidal aggregation and streamer fouling have a significant effect on overall membrane fouling which were not studied before.
Parvalbumin expression in oligodendrocyte-like CG4 cells causes a reduction in mitochondrial volume, attenuation in reactive oxygen species production and a decrease in cell processes’ length and branching
Authors: Lichvarova Lucia , Blum Walter , Schwaller Beat , Szabolcsi Viktoria 
Published: July 2019

AbstractForebrain glial cells - ependymal cells and astrocytes -acquire upon injury- a “reactive” phenotype associated with parvalbumin (PV) upregulation. Since free radicals, e.g. reactive oxygen species (ROS) play a role in the pathogenesis of multiple sclerosis, and that PV-upregulation in glial cells is inversely correlated with the level of oxidative stress, we hypothesized that PV-upregulation might also protect oligodendrocytes by decreasing ROS production. Lentiviral transduction techniques allowed for PV overexpression in CG4 oligodendrocyte progenitor cells (OPCs). Depending on the growth medium CG4 cells can be maintained in an OPC-like state, or induced to differentiate into an oligodendrocyte (OLG)-like phenotype. While increased levels of PV had no effect on cell proliferation and invasiveness in vitro, PV decreased the mitochondria volume in CG4 cell bodies, as well as the mitochondrial density in CG4 processes in both OPC-like and OLG-like states. In line with the PV-induced global decrease in mitochondrial volume, elevated PV levels reduced transcript levels of mitochondrial transcription factors involved in mitochondria biogenesis. In differentiated PV-overexpressing CG4 cells with a decreased mitochondrial volume, UV-induced ROS production was lower than in control CG4 cells hinting towards a possible role of PV in counteracting oxidative stress. Unexpectedly, PV also decreased the length of processes in undifferentiated CG4 cells and moreover diminished branching of differentiated CG4 cell processes, strongly correlated with the decreased density of mitochondria in CG4 cell processes. Thus besides conferring a protective role against oxidative stress, PV in a cell autonomous fashion additionally affects process’ growth and branching in CG4 cells.
Three-dimensional HepaRG spheroids as a liver model to study human genotoxicity in vitro with the single cell gel electrophoresis assay
Authors: Mandon Marion , Huet Sylvie , Dubreil Estelle , Fessard Valérie , Le Hégarat Ludovic 
Published: July 2019

AbstractMany efforts have been made in the last 30 years to develop more relevant in vitro models to study genotoxic responses of drugs and environmental contaminants. While 2D HepaRG cells are one of the most promising models for liver toxicology, a switch to 3D cultures that integrate both in vivo architecture and cell-cell interactions has occurred to achieve even more predictive models. Preliminary studies have indicated that 3D HepaRG cells are suitable for liver toxicity screening. Our study aimed to evaluate the response of HepaRG spheroids exposed to various genotoxic compounds using the single cell gel electrophoresis assay. HepaRG spheroids were used at 10 days after seeding and exposed for 24 and 48 hours to certain selected chemical compounds (methylmethansulfonate (MMS), etoposide, benzo[a]pyrene (B[a]P), cyclophosphamide (CPA), 7,12-dimethylbenz[a]anthracene (DMBA), 2-acetylaminofluorene (2-AAF), 4-nitroquinoline (4-NQO), 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), 2-amino-3-methylimidazo[4,5-f]quinolone (IQ), acrylamide, and 2-4-diaminotoluene (2,4-DAT)). After treatment, the comet assay was performed on single cell suspensions and cytotoxicity was determined by the ATP assay. Comet formation was observed for all compounds except IQ, etoposide and 2,4-DAT. Treatment of spheroids with rifampicin increased CYP3A4 activity, demonstrating the metabolic capacity of HepaRG spheroids. These data on genotoxicity in 3D HepaRG spheroids are promising, but further experiments are required to prove that this model can improve the predictivity of in vitro models to detect human carcinogens.
Trip Centrality: walking on a temporal multiplex with non-instantaneous link travel time
Authors: Zaoli Silvia , Mazzarisi Piero , Lillo Fabrizio 
Published: July 2019

AbstractIn complex networks, centrality metrics quantify the connectivity of nodes and identify the most important ones in the transmission of signals. In many real world networks, especially in transportation systems, links are dynamic, i.e. their presence depends on time, and travelling between two nodes requires a non-vanishing time. Additionally, many networks are structured on several layers, representing, e.g., different transportation modes or service providers. Temporal generalisations of centrality metrics based on walk-counting, like Katz centrality, exist, however they do not account for non-zero link travel times and for the multiplex structure. We propose a generalisation of Katz centrality, termed Trip Centrality, counting only the walks that can be travelled according to the network temporal structure, i.e. “trips”, while also differentiating the contributions of inter- and intra-layer walks to centrality. We show an application to the US air transport system, specifically computing airports’ centrality losses due to delays in the flight network.
Mammographic casting-type calcification is an independent prognostic factor in invasive breast cancer
Authors: Li Yan , Cao Jian , Zhou Yidong , Mao Feng , Shen Songjie , Sun Qiang 
Published: July 2019

AbstractThis study aimed to determine whether there is an association between mammographic casting-type calcification and other prognostic factors for invasive breast cancer. We also assessed whether casting-type calcification could be an independent prognostic factor. Invasive breast cancer patient information from January 2010 and January 2013 was retrospectively reviewed. The associations between mammographic casting-type calcification and other clinicopathological factors, including tumor size, node status, grade, progesterone receptor (PR) status, estrogen receptor (ER) status, and human epidermal growth factor receptor 2 (HER2) status, were analyzed. The Kaplan–Meier method and a Cox proportional hazards model were used for survival analyses of disease-free survival (DFS) and overall survival (OS). A total of 1155 invasive breast cancer patients who underwent definitive surgery were included, and 136 cases (11.8%) had casting-type calcification on mammography. In multivariate logistic regression, casting-type calcification was significantly associated with axillary node metastasis, ER-negativity, and HER2 overexpression. Casting-type calcification significantly decreased OS and DFS after a median follow-up of 60 months. This result remained after adjusting other prognostic factors in the multivariate analysis. Casting-type calcification is significantly linked to axillary node metastasis, ER-negativity and HER2 overexpression. Casting-type calcification is therefore an independent prognostic factor for breast cancer patients.
Monte-Carlo dosimetry and real-time imaging of targeted irradiation consequences in 2-cell stage Caenorhabditis elegans embryo
Authors: Torfeh Eva , Simon Marina , Muggiolu Giovanna , Devès Guillaume , Vianna François , Bourret Stéphane , Incerti Sébastien , Barberet Philippe , Seznec Hervé 
Published: July 2019

AbstractCharged-particle microbeams (CPMs) provide a unique opportunity to investigate the effects of ionizing radiation on living biological specimens with a precise control of the delivered dose, i.e. the number of particles per cell. We describe a methodology to manipulate and micro-irradiate early stage C. elegans embryos at a specific phase of the cell division and with a controlled dose using a CPM. To validate this approach, we observe the radiation-induced damage, such as reduced cell mobility, incomplete cell division and the appearance of chromatin bridges during embryo development, in different strains expressing GFP-tagged proteins in situ after irradiation. In addition, as the dosimetry of such experiments cannot be extrapolated from random irradiations of cell populations, realistic three-dimensional models of 2 cell-stage embryo were imported into the Geant4 Monte-Carlo simulation toolkit. Using this method, we investigate the energy deposit in various chromatin condensation states during the cell division phases. The experimental approach coupled to Monte-Carlo simulations provides a way to selectively irradiate a single cell in a rapidly dividing multicellular model with a reproducible dose. This method opens the way to dose-effect investigations following targeted irradiation.
An ICCD camera-based time-domain ultrasound-switchable fluorescence imaging system
Authors: Yu Shuai , Yao Tingfeng , Yuan Baohong 
Published: July 2019

AbstractFluorescence imaging in centimeter-deep tissues with high resolution is highly desirable for many biomedical applications. Recently, we have developed a new imaging modality, ultrasound-switchable fluorescence (USF) imaging, for achieving this goal. In our previous work, we successfully achieved USF imaging with several types of USF contrast agents and imaging systems. In this study, we introduced a new USF imaging system: an intensified charge-coupled device (ICCD) camera-based, time-domain USF imaging system. We demonstrated the principle of time-domain USF imaging by using two USF contrast agents. With a series of USF imaging experiments, we demonstrated the tradeoffs among different experimental parameters (i.e., data acquisition time, including CCD camera recording time and intensifier gate delay; focused ultrasound (FU) power; and imaging depth) and the image qualities (i.e., signal-to-noise ratio, spatial resolution, and temporal resolution). In this study, we also discussed several imaging strategies for achieving a high-quality USF image via this time-domain system.
Cortical-wide functional correlations are associated with stress-induced cardiac dysfunctions in individual rats
Authors: Nakayama Ryota , Ikegaya Yuji , Sasaki Takuya 
Published: July 2019

AbstractMental stress-induced biological responses considerably differ across animals, which may be explained by intrinsic brain activity patterns. To address this hypothesis, we recorded local field potential signals from six cortical areas, electrocardiograms, and electromyograms from freely moving rats. Based on their stress-induced changes in cardiac signals, individual defeated rats were classified into stress susceptible and resilient groups. Rats with lower correlations in theta power across wide ranges of cortical regions before the stress challenge had higher probability to be stress-susceptible rats as defined based on the irregularity of heartbeat signals. A combination of principal component analysis and the support vector machine algorithm revealed that functional connections across cortical regions could be predictive factors accounting for individual differences in future stress susceptibility. These results suggest that individual differences in cortical activity may be a mechanism that causes abnormal activity of peripheral organs in response to mental stress episodes. This evidence will advance the understanding of the neurophysiological correlates of mind-body associations during mental stress exposure.
Vps10-mediated targeting of Pep4 determines the activity of the vacuole in a substrate-dependent manner
Authors: Boutouja Fahd , Stiehm Christian M. , Mastalski Thomas , Brinkmeier Rebecca , Reidick Christina , El Magraoui Fouzi , Platta Harald W. 
Published: July 2019

AbstractThe vacuole is the hydrolytic compartment of yeast cells and has a similar function as the lysosome of higher eukaryotes in detoxification and recycling of macromolecules. We analysed the contribution of single vacuolar enzymes to pexophagy and identified the phospholipase Atg15, the V-ATPase factor Vma2 and the serine-protease Prb1 along with the already known aspartyl-protease Pep4 (Proteinase A) to be required for this pathway. We also analysed the trafficking receptor Vps10, which is required for an efficient vacuolar targeting of the precursor form of Pep4. Here we demonstrate a novel context-dependent role of Vps10 in autophagy. We show that reduced maturation of Pep4 in a VPS10-deletion strain affects the proteolytic activity of the vacuole depending on the type and amount of substrate. The VPS10-deletion has no effect on the degradation of the cytosolic protein Pgk1 via bulk autophagy or on the degradation of ribosomes via ribophagy. In contrast, the degradation of an excess of peroxisomes via pexophagy as well as mitochondria via mitophagy was significantly hampered in a VPS10-deletion strain and correlated with a decreased maturation level of Pep4. The results show that Vps10-mediated targeting of Pep4 limits the proteolytic capacity of the vacuole in a substrate-dependent manner.
Mobility assessment using wearable technology in patients with late-onset Pompe disease
Authors: Hamed Alaa , Curran Christopher , Gwaltney Chad , DasMahapatra Pronabesh 
Published: July 2019

AbstractLate-onset Pompe disease (LOPD) is a rare genetic disorder due to the absence or deficiency of acid alpha-glucosidase enzyme resulting in slowly progressing reduction of muscle strength, causing difficulties with mobility and respiration. Wearable technologies offer novel options to evaluate mobility in a real-world setting. LOPD patients self-reporting LOPD, ≥18 years, US residents, walking (with or without aid), and not on invasive ventilation were recruited for a 6- to 8-week wearable study via patient organizations. Eligible patients were shipped a wearable tracker (Fitbit One™) and completed self-assessment questionnaires. Mobility outcome measures were median step count and peak 1-min activity. In the analyses cohort (N = 29), engagement in data sharing was high (94% of patients uploaded data for more than half the study days). Mean age was 43 years, 90% were females, and 93% were diagnosed in adulthood. Mean delay in diagnosis was 10 years; most had disease onset for ≥10 years (55%); some required walking aid (17%) and breathing assistance (38%). Mean step count differed by age (20–39 years: 4071 vs. 40–69 years: 2394,p < 0.01), diagnostic delay (<10 years: 3584 vs. ≥10 years: 2232,p < 0.05), disease duration (<10 years: 4219 vs. ≥10 years: 2462,p < 0.05), and ambulatory status (aided: 1883 vs. unaided: 3408,p < 0.05). Patient-reported “fatigue and pain” score was inversely correlated with step count (Pearson’sr = −0.42,p < 0.05) and peak 1-min activity (Pearson’sr = −0.49,p < 0.01). This study illustrates a new approach to measure mobility in LOPD patients and establishes a framework for future outcomes data collection.
Wearable sensors for monitoring the physiological and biochemical profile of the athlete
Authors: Seshadri Dhruv R. , Li Ryan T. , Voos James E. , Rowbottom James R. , Alfes Celeste M. , Zorman Christian A. , Drummond Colin K. 
Published: July 2019

AbstractAthletes are continually seeking new technologies and therapies to gain a competitive edge to maximize their health and performance. Athletes have gravitated toward the use of wearable sensors to monitor their training and recovery. Wearable technologies currently utilized by sports teams monitor both the internal and external workload of athletes. However, there remains an unmet medical need by the sports community to gain further insight into the internal workload of the athlete to tailor recovery protocols to each athlete. The ability to monitor biomarkers from saliva or sweat in a noninvasive and continuous manner remain the next technological gap for sports medical personnel to tailor hydration and recovery protocols per the athlete. The emergence of flexible and stretchable electronics coupled with the ability to quantify biochemical analytes and physiological parameters have enabled the detection of key markers indicative of performance and stress, as reviewed in this paper.
Making Sense of Sentences: Top-Down Processing of Speech by Adult Cochlear Implant Users
Authors: Moberly Aaron C. , Reed Jessa 
Published: July 2019

Purpose
Speech recognition relies upon a listener's successful pairing of the acoustic–phonetic details from the bottom-up input with top-down linguistic processing of the incoming speech stream. When the speech is spectrally degraded, such as through a cochlear implant (CI), this role of top-down processing is poorly understood. This study explored the interactions of top-down processing, specifically the use of semantic context during sentence recognition, and the relative contributions of different neurocognitive functions during speech recognition in adult CI users.

Method

Data from 41 experienced adult CI users were collected and used in analyses. Participants were tested for recognition and immediate repetition of speech materials in the clear. They were asked to repeat 2 sets of sentence materials, 1 that was semantically meaningful and 1 that was syntactically appropriate but semantically anomalous. Participants also were tested on 4 visual measures of neurocognitive functioning to assess working memory capacity (Digit Span;
Wechsler, 2004
), speed of lexical access (Test of Word Reading Efficiency;
Torgeson, Wagner, & Rashotte, 1999
), inhibitory control (Stroop;
Stroop, 1935
), and nonverbal fluid reasoning (Raven's Progressive Matrices;
Raven, 2000
).

Results
Individual listeners' inhibitory control predicted recognition of meaningful sentences when controlling for performance on anomalous sentences, our proxy for the quality of the bottom-up input. Additionally, speed of lexical access and nonverbal reasoning predicted recognition of anomalous sentences.

Conclusions
Findings from this study identified inhibitory control as a potential mechanism at work when listeners make use of semantic context during sentence recognition. Moreover, speed of lexical access and nonverbal reasoning were associated with recognition of sentences that lacked semantic context. These results motivate the development of improved comprehensive rehabilitative approaches for adult patients with CIs to optimize use of top-down processing and underlying core neurocognitive functions.
Neighborhood Density and Word Production in Delayed and Advanced Learners
Authors: Jones Samuel David , Brandt Silke 
Published: July 2019

Purpose

This study reexamines the claim that difficulty forming memories of words comprising uncommon sound sequences (i.e., low phonological neighborhood density words) is a determinant of delayed expressive vocabulary development (e.g.,
Stokes, 2014
).

Method

We modeled communicative development inventory data from (
N
= 442) 18-month-old children, with expressive lexicon sizes between 0 and 517 words (
Mdn
= 84). We fitted a Bayesian regression model in which the production of each communicative inventory word (
N
= 680) by each child was predicted by interactions between that child's expressive lexicon size and the word's (a) phonological neighborhood density, (b) frequency in child-directed speech, (c) length, (d) babiness, and (e) concreteness.

Results
Children with larger expressive lexicons were more likely to produce words comprising uncommon sound sequences than age-matched children with smaller lexicons. However, the magnitude of the interaction between expressive lexicon size and phonological neighborhood density was modest relative to interactions between expressive lexicon size and word frequency, length, babiness, and concreteness.

Conclusion
Emphasis on a difficulty with the memorization of low–neighborhood density words as a determinant of slow vocabulary growth may be unwarranted, and the current evidence base in this direction is not robust enough to strongly support the development of possible interventions for late talkers.