bluuebunny/crossref_metadata_2025_split huggingface dataset:
Satiety, Thermosensation and Mechanosensation Regulate a Spontaneous C. elegans Sleep State
Authors: Gonzales Daniel L. , Zhou Jasmine , Robinson Jacob T. 
Published: February 2019

AbstractOne remarkable feature of the nervous system is its ability to rapidly and spontaneously switch between activity states. In the extreme example of sleep, animals arrest locomotion, reduce their sensitivity to sensory stimuli, and dramatically alter their neural activity. Small organisms are useful models to better understand these sudden changes in neural states because we can simultaneously observe whole-brain activity, monitor behavior and precisely regulate the external environment. Here, we show a spontaneous sleep-like behavior in C. elegans that is associated with a distinct global-brain state and regulated by both the animal’s internal physiological state and input from multiple sensory circuits. Specifically, we found that when confined in microfluidic chambers, adult worms spontaneously transition between periods of normal activity and short quiescent bouts, with behavioral state transitions occurring every few minutes. This quiescent state, which we call μSleep, meets the behavioral requirements of C. elegans sleep, is dependent on known sleep-promoting neurons ALA and RIS, and is associated with a global down-regulation of neural activity. Consistent with prior studies of C. elegans sleep, we found that μSleep is regulated by satiety and temperature. In addition, we show for the first time that quiescence can be either driven or suppressed by thermosensory input, and that animal restraint induces quiescence through mechanosensory pathways. Together, these results establish a rich model system for studying how neural and behavioral state transitions are influenced by multiple physiological and environmental conditions.Significance StatementUnique brain states govern animal behaviors like sleep and wakefulness; however, how the brain regulates these dramatic state transitions is not well understood. Brain activity can be influenced by a complex interaction between sensory circuits that monitor the external environment, neural circuits that control behavior, and internal chemical signaling. Here, we describe a platform to study behavioral states in a context that allows us to record whole-brain activity while controlling the environment and monitoring animal behavior. Specifically, we identify a pattern of sleep bouts in the roundworm C. elegans that occur when they are confined to microscopic fluidic chambers. This behavior platform provides a powerful system to study how neural circuits interact with chemical signaling to drive brain state transitions.
The kinetics of nucleotide binding to isolatedChlamydomonasaxonemes using UV-TIRF microscopy
Authors: Feofilova M. , Mahamdeh M. , Howard J. 
Published: February 2019

AbstractCilia and flagella are long, slender organelles found in many eukaryotic cells where they have sensory, developmental and motile functions. All cilia and flagella contain a microtubule-based structure called the axoneme. In motile cilia and flagella, which drive cell locomotion and fluid transport, the axoneme contains, along most of its length, motor proteins from the axonemal dynein family. These motor proteins drive motility by using energy derived from the hydrolysis of ATP to generate a bending wave, which travels down the axoneme. As a first step towards visualizing the ATPase activity of the axonemal dyneins during bending, we have investigated the kinetics of nucleotide binding to axonemes. Using a specially built UV-TIRF microscope, we found that the fluorescent ATP analog mantATP (methylanthraniloyl adenosine triphosphate), which has been shown to support axonemal motility, binds all along isolated, immobilized axonemes. By studying the recovery of fluorescence after photobleaching, we found that there are three mantATP binding sites: one that bleaches rapidly (time constant ≈ 1.7 s) and recovers slowly (time constant ≈ 44 s), one that bleaches with the same time constant but does not recover and one that does not bleach. By reducing the dynein content in the axoneme using mutants and salt extraction, we provide evidence that the slow-recovering component corresponds to axonemal dyneins. The recovery rate of this component, however, is too slow to be consistent with the activation of beating observed at higher mantATP concentrations; this indicates that the dyneins may be inhibited due to their immobilization at the surface. The development of this method is a first step towards direct observation of the travelling wave of dynein activity.
Coinfection of semi-infectious particles can contribute substantially to influenza infection dynamics
Authors: Farrell Alex , Brooke Christopher , Koelle Katia , Ke Ruian 
Published: February 2019

AbstractInfluenza is an RNA virus with a genome comprised of eight gene segments. Recent experiments show that the vast majority of virions fail to express one or more gene segments and thus cannot cause a productive infection on their own. These particles, called semi-infectious particles (SIPs), can induce virion production through complementation when multiple SIPs are present in an infected cell. Previous within-host influenza models ignore the potential effects of coinfection and SIPs during virus infection. Here, to investigate the extent that SIPs and coinfection impact viral dynamics, we constructed two within-host models that explicitly keep track of SIPs and coinfection, and fitted the models to clinical data published previously. We found that the model making a more realistic assumption that viruses can only reach a limited number of target cells allows for frequent co-infection during early viral exponential growth and predicts that SIPs contribute substantially to viral load. Furthermore, the model provides a new interpretation of the determinants of viral growth and predicts that the virus within-host growth rate (a measure of viral fitness) is relatively insensitive to the fraction of virions being SIPs, consistent with biological observations. Our results highlight the important role that cellular co-infection can play in regulating infection dynamics and provide a potential explanation for why SIP production is not highly deleterious. More broadly, the model can be used as a general framework to understand coinfection/superinfection in other viral infections.Author SummaryInfluenza A viruses (IAVs) represent a large public health burden across the world. Currently, our understanding of their infection dynamics is incomplete, which hinders the development of effective vaccines and treatment strategies. Recently, it was shown that a large fraction of virions, called semi-infectious particles, do not cause productive infection on their own; however, coinfection of these particles leads to productive infection. The extent that semi-infectious particles and, more broadly, coinfection contribute to overall influenza infection dynamics is not clear. To address this question, we constructed mathematical models explicitly keeping track of semi-infectious particles and coinfection. We show that coinfection can be frequent over the course of infection and that SIPs play an important role in regulating infection dynamics. Our results have implications towards developing effective therapeutics.
Entrepreneurial competencies and SMEs’ growth: the mediating role of network competence
Authors: Tehseen Shehnaz , Ahmed Farhad Uddin , Qureshi Zuhaib Hassan , Uddin Mohammad Jasim , T. Ramayah 
Published: February 2019

PurposeThe purpose of this paper is twofold. First, to examine the influence of two dimensions of entrepreneurial competencies, namely, strategic competency and ethical competency on the growth of small and medium enterprises (SMEs); and second, to explore the role of network competence as a mediator among these understudied variables.Design/methodology/approachThe data for this study were collected from wholesale and retail-based SMEs in Malaysia through a standard structured questionnaire. PLS–SEM approach was utilised to analyse the data.FindingsAlthough the findings did not reveal a direct effect of strategic and ethical competencies of entrepreneurs on SMEs’ growth, these competences, however, were found to be influential in driving their growth when network competence was used as a mediator.Originality/valueThe existence of a mediation effect between strategic and ethical competencies and SMEs’ growth via network competence has provided insights which add new knowledge to the extant entrepreneurship and SMEs’ performance literature.
To investigate the determinants of cloud computing adoption in the manufacturing micro, small and medium enterprises
Authors: Raut Rakesh D. , Gardas Bhaskar B. , Narkhede Balkrishna E. , Narwane Vaibhav S. 
Published: February 2019

Purpose
The purpose of this paper is to identify the critical factors influencing the cloud computing adoption (CCA) in the manufacturing micro, small and medium enterprises (MSMEs) by employing a decision-making trial and evaluation laboratory (DEMATEL) methodology.

Design/methodology/approach
Through literature review and expert opinions, 30 significant factors were identified, and then a DEMATEL approach was applied for exploring the cause–effect relationship between the factors.

Findings
The results of study highlighted that five factors, namely, “hardware scalability and standardisation”, “cost (subscription fees, maintenance cost and implementation cost (CS1)”, “innovation”, “installation and up gradation (CS28)”, and “quality of service” were the most significant factors influencing the CCA in the case sector.

Research limitations/implications
The DEMATEL model was developed by considering expert inputs, and these inputs could be biased which can influence the reliability of the model. This study guides the organisational managers, cloud service providers and governmental organisations in formulating the new policies/strategies or modifying the existing ones for the effective CCA in the case sector.

Originality/value
For the first time. interdependency between the critical factors influencing CCA was discussed by employing the DEMATEL approach in the Indian manufacturing MSMEs context.
The adoption of soft skills in supply chain and understanding their current role in supply chain management skills agenda
Authors: Bak Ozlem , Jordan Christine , Midgley James 
Published: February 2019

PurposeWith supply chains expanding in scope and scale globally, the academic literature underlined the increasing role and importance of soft skills. Traditionally, the supply chain literature geared towards hard skills including functional and technical skill sets with limited discussion on soft skills. Therefore, the purpose of this paper is to assess and explore the soft skills demand in supply chain management arena.Design/methodology/approachThis study has utilised a mixed methods study in two phases, with the first stage including a questionnaire distributed to 120 supply chain employees in the UK, followed by six interviews with supply chain experts in the UK.FindingsThe results suggest that soft skills, especially behavioural skills such as communication, planning, initiative and negotiation, were seen to be more important when compared to decision making, negotiation and management skills. The findings indicate that the changing supply chain scope encourages the requisition and development of different supply chain soft skills with varied levels of emphasis in relation to 15 soft skills identified in the literature.Research limitations/implicationsThis study employs a mixed-method approach to establish the perceived importance of soft skills in the UK supply chains. This limits the generalisability of the results to other contextual settings.Practical implicationsThis paper presents soft skills impact upon the supply chain. Specific soft skills are critical to supply chain employees compared to others (e.g. behavioural and people management skills), which may lead to articulation of supply chain soft skills training initiatives.Originality/valueThis paper contributes to the soft skills discussion in the supply chain context and discusses the role of soft skills. Topical gaps in the literature are identified as areas for future research. The findings have generated additional supply chain skills to the academic literature as well as provided an understanding of the weighting of soft skills in terms of their importance and application to industry needs.
Construction logistics governing guidelines in urban development projects
Authors: Janné Mats , Fredriksson Anna 
Published: February 2019

Purpose
Although a construction logistics solution is necessary for dealing with the demands in many large urban development projects, there is a lack of research on governance mechanisms for construction logistics solutions. Therefore, the purpose of this paper is to analyse the initiation and utilisation of a construction logistics centre (CLC) from different stakeholders’ perspectives to suggest governance mechanisms for strategic, tactical and operational levels and to develop guidelines for implementing these governance mechanisms.

Design/methodology/approach
A single case research design was used. Data were collected through interviews, site visits, observations and documentation from four stakeholder groups.

Findings
There is potential for utilising CLCs in development projects, with positive effects such as consolidation effects and enhanced planning. What is evident, however, is that the design and implementation of the CLCs must be based on a comprehensive stakeholder analysis, as there are conflicting goals between stakeholders. Governance mechanisms, including flexibility in the main contractors’ working construction process, as well as clearly stated roles, responsibilities and communication must be developed to enhance this potential.

Research limitations/implications
The conflicting goals of CLCs are identified and discussed, and the results show the need for further multi-stakeholder analysis of construction logistics solutions.

Practical implications
The experiences from the studied case are developed into practical guidelines to be used in the design of construction logistics solutions in development projects.

Originality/value
This study contributes by taking a multi-stakeholder perspective on CLCs and providing guidelines to be used in the design of construction logistics solutions in development projects.
Development and validation of financial well-being related scales
Authors: Abrantes-Braga Farah Diba M.A. , Veludo-de-Oliveira Tania 
Published: February 2019

PurposeThe purpose of this paper is to develop valid and reliable scales for assessing a driver and two obstacles potentially related to financial well-being (FWB): financial preparedness for emergency, beliefs of credit limits as additional income and risky indebtedness behaviour.Design/methodology/approachThe scales were developed from scratch across six studies, employing a two-step methodology, which encompassed both qualitative (e.g. focus group, interviews) and quantitative (i.e. online surveys) data collection. Exploratory and confirmatory factor analyses were employed to test and validate the proposed scales.FindingsThis study provides a set of three parsimonious, self-reported behavioural measures that could be employed in conjunction with objective economic indicators to identify individuals who are financially ill prepared and potential candidates for delinquency. The three proposed scales achieved satisfactory levels of reliability and convergent and discriminant validity.Research limitations/implicationsThe resulting scales still need to be tested for predictive validity and in different consumer groups. The scales were validated in a single culture population (Brazil, a country that presents extraordinarily high credit card interest rates), and they should be tested cross-culturally in countries with different economic and credit policies.Originality/valueThe literature on FWB has traditionally employed objective financial indicators as an attempt to measure the concept of FWB and its elements. Self-reported behavioural measures of such constructs are scant to the point of being non-existent for some elements. This study is the first to offer scales for measuring the elements of financial preparedness for emergency, beliefs of credit limits as additional income and risky indebtedness behaviour.
Credit card literacy and financial well-being of college students
Authors: Limbu Yam B. , Sato Shintaro 
Published: February 2019

Purpose
By testing a moderated mediation model, the purpose of this paper is to examine the mediating role of credit card self-efficacy in the relationship between credit card literacy and financial well-being. The authors further examine if credit card number moderates this effect.

Design/methodology/approach
Data for the study were collected from 427 college students. The PROCESS macros in IBM SPSS Statistics 23 was used to assess the hypothesized relationships.

Findings
Credit card literacy positively influences financial well-being through self-efficacy. However, this effect is stronger when college students own fewer credit cards.

Practical implications
Banks and credit card issuers, policymakers and colleges and universities should place a greater emphasis on credit card literacy programs that enhance students’ general understanding of credit card terms and conditions and confidence in their ability to effectively use and manage their credit cards.

Originality/value
To our knowledge, this is the first study to examine the relationship between credit card literacy, self-efficacy and financial well-being.
Multilevel latent class modeling to segment the microfinance market
Authors: Souza Lucas Lopes Ferreira , Bassi Francesca , Ferreira de Freitas Ana Augusta 
Published: February 2019

Purpose
Microfinance has become an important way to alleviate poverty. Though four decades have passed since its introduction, its impact is still not entirely clear. What makes it difficult to ascertain its efficacy is the existence of diverse types of microfinance organizations and client profiles. Microfinance institutions must primarily pay more attention to the client, and to the mechanism through which financial services are delivered. The purpose of this paper is to identify the profiles of microfinance customers and the features of their operations.

Design/methodology/approach
In this paper, multilevel latent class models were estimated to reveal clusters of operations and classes of clients.

Findings
The results show that there are six clusters of operations and four classes of clients in the market, each with distinct profiles and needs. Different strategies are recommended for each cluster and class.

Originality/value
Numerous studies have focused on the importance of getting to know the clients of microfinance programs, but none as yet have used market segmentation as a way to do so. The goal is to generate better strategies to help clients improve their business results. Applying market segmentation to the microfinance market may point to different products for different groups of clients, taking the real needs of each of them into account.
Adoption of mobile banking services
Authors: Giovanis Apostolos , Athanasopoulou Pinelopi , Assimakopoulos Costas , Sarmaniotis Christos 
Published: February 2019

PurposeThe purpose of this paper is to investigate which of four well-established theoretical models (i.e. technology acceptance model, theory of planned behavior, unified theory of acceptance and use of technology, decomposed theory of planned behavior (DTPB)) best explains potential users’ behavioral intentions to adopt mobile banking (MB) services.Design/methodology/approachDrawing on data from 931 potential users in Greece, the structural equation modeling method was used to examine and compare the four models in goodness-of-fit, explanatory power and statistical significance of path coefficients.FindingsResults indicate that the best model is an extension of the DTPB with perceived risk (PR). Customers’ attitude, determined by three rationally-evaluated MB attributes (usefulness, easiness and compatibility), is the main driver of consumers’ intentions to adopt MB services. Additionally, consumers’ perceptions of availability of knowledge, resources and opportunities necessary for using the service, and the pressure of interpersonal and external social contexts toward the use of MB are the other two, less important, adoption drivers. Finally, PR negatively affects attitude formation and inhibits willingness to use MB services.Practical implicationsFindings can help marketers of financial institutions to select the more parsimonious model to develop appropriate marketing strategies to increase adoption rates of MB services.Originality/valueThis is the first study that compares the performance of four well-known innovation adoption models to explain consumers’ behavior in the MB context.
Motivations of undertaking CSR initiatives by independent hotels: a holistic approach
Authors: Abaeian Vahideh , Khong Kok Wei , Kyid Yeoh Ken , McCabe Scott 
Published: February 2019

PurposeThe purpose of this study is to explore how senior managers of independent hotels perceive the notion of corporate social responsibility (CSR) through a holistic analysis of motivations of undertaking both social and environmental initiatives.Design/methodology/approachThis study conducts qualitative interviews with 22 Malaysian senior hotel managers together with ethnographic observations.FindingsEmergent themes show that CSR is a dynamic concept involving complex struggles and trade-offs between fulfilling business objectives, paying heed to personal ethical values and considering cultural norms when making decisions regarding the adoption of a range of environmental and social initiatives.Research limitations/implicationsThis study contributes to legitimacy theory by highlighting that in the absence of pressure from key stakeholders for responsible initiatives, managers still proactively engage in CSR initiatives. More surprising still is that they set expectations instead of simply aligning or responding to the key stakeholders’ pre-set demands. Meanwhile, the uniqueness, size and purposeful selection of the sample limit the applicability of the findings to wider geographical and cultural locations.Practical implicationsWhile Malaysian hotel managers are willing to use CSR practices to demonstrate their commitment to their employees and the local communities where they operate, it is equally important that they utilize other media (such as their websites or social media) for both promotional and legitimacy building purposes. Within the context of growing tourism for economic development, the Malaysian Government can develop more effective strategies, rewards or incentives for encouraging businesses to undertake and improve CSR adoption for sustainability of this industry.Originality/valueBy extending the scope of CSR studies beyond firm performance issues, we attempt to show the wider set of motivations and contexts considered important in determining hotel engagement in CSR programs.