bluuebunny/crossref_metadata_2025_split huggingface dataset:
Núcleo de Estudo Geempiano do Distrito Federal: um olhar “de dentro para fora”
Authors: Oliveira Ândrea De Queiroz , Da Rocha Carla Borba , Medeiros Marques Yone Martins , Tuboiti Nair Cristina Da Silva 
Published: November 2018

<span>Diante de altos índices de analfabetismo, principalmente no que se refere a alunos oriundos de classes populares, em torno de 50% ao final de três anos de estudos, ou seja, crianças aos 8 anos de idade, nosso questionamento se direciona para a necessidade de mudança em busca da qualidade na educação no Ensino Fundamental, prioritariamente nas escolas públicas. Sendo assim, esse relato de experiência objetiva abordar aspectos estruturais da formação e as interferências no processo de aprendizagem dos professores ao ensinarem seus alunos, chegando, ao final de cada ano letivo, a índices de 100% de alfabetização em suas turmas. Nosso estudo apresenta o relato de profissionais pertencentes ao Núcleo de Estudo Geempiano do Distrito Federal que, composto por professoras vinculadas à Secretaria de Educação do Distrito Federal, ao longo de 13 anos, vem construindo uma trajetória de profissionalização enquanto alfabetizadoras. Utilizam-se da proposta didático-pedagógica pós-construtivista, que tem como ponto de partida, o princípio da igualdade das inteligências e o processo psicogenético de cada aluno, dentro de um Campo Conceitual de conhecimentos relacionados à leitura e à escrita. Comprovamos a formação de professores alfabetizadores, junto à proposta didático-pedagógica pós-construtivista, como uma opção na busca da melhoria e da democratização das aprendizagens, uma vez que possuem uma trajetória bem sucedida de alfabetização em turmas de classes populares.</span>
Narrativas sobre a Centralidade da Mulher numa Sociedade Atual: Perspectivas Bororo
Authors: Campos Neide Da Silva , Grando Beleni Saléte 
Published: November 2018

<p>Neste artigo, fruto de uma pesquisa de doutorado em andamento que visa compreender as trajetórias das professoras da etnia Bororo na educação escolar indígena, trazemos as primeiras aproximações da temática a partir do diálogo com mulheres e liderança a fim de pensar as relações entre os diferentes papeis da mulher professora e mulher bororo considerando sua estrutura social matrilinear. Apresentaremos neste ensaio, dados preliminares a partir de três fontes de dados distintas, que nos permitem compreender o lugar da mulher Boe a partir da cosmovisão Bororo e nisso analisar sentidos e significados atribuídos às mulheres na sociedade Bororo, considerando toda a especificidade da organização social e cultural do povo cuja estrutura social é centrada na mulher. A educação tradicional articula os mundos que constituem a cosmologia Bororo, na qual se vinculam as dimensões material e imaterial num processo binário de estruturação e organização social, a fim de educar o corpo-ser <em>aredu</em> (mulher) e o corpo-ser <em>imedu</em> (homem). Na concepção Bororo de ver o mundo, há sempre duas metades, dois lados, que não são antagônicos, mas complementares e co-dependentes, no sentido de que um clã, uma metade exogâmica, não pode sobreviver sem a outra, pois uma dá visibilidade à outra, permitindo o reconhecimento de diferença e pertencimento de cada pessoa na sociedade a partir do seu grupo social clânico matrilinear.</p><p> </p>
The role of synthetic P (MMA‐co‐MAH) as compatibilizer in the preparation of chlorinated polyethylene/polysodium acrylate water‐swelling rubber
Authors: Fang Zhengping , Zhang Xingning , Xia Meng , Luo Wei , Hu Huiling , Wang Zhiguo , He Peixin , Zhang Yuhong 
Published: November 2018

AbstractBy multicomponent mechanical blending technology, water‐swelling rubber (WSR) was prepared through choosing chlorinated polyethylene (CPE), a superabsorbent polymer (cross‐linking poly(sodium acrylate), CPNaAA), and compatibilizer (poly(methyl methacrylate‐co‐maleic anhydride), P (MMA‐co‐MAH)) along with other minor additives. P (MMA‐co‐MAH) was synthesized by grafting maleic anhydride (MAH) onto methyl methacrylate (MMA). Microstructure, water‐absorbent properties, and mechanical properties of WSR were investigated. The results showed that P (MMA‐co‐MAH) boosted the dispersibility between CPNaAA and CPE and led to the increase in Shore A hardness, tensile strength, tensile stress at 100%, and water‐swelling behavior, whereas the elongation at break and weight loss were decreased with P (MMA‐co‐MAH) content. When the content of P (MMA‐co‐MAH) reached 8 phr, the tensile strength, hardness, and water‐swelling ability of dry‐state WSR exhibited a maximum value and the percentage loss ratio of CPNaAA reached a relatively low value.
Genetically inspired prognostic scoring system (GIPSS) outperforms dynamic international prognostic scoring system (DIPSS) in myelofibrosis patients
Authors: Kuykendall Andrew T. , Talati Chetasi , Padron Eric , Sweet Kendra , Sallman David , List Alan F. , Lancet Jeffrey E. , Komrokji Rami S. 
Published: November 2018

AbstractA genetically inspired prognostic scoring system (GIPSS) that stratifies primary myelofibrosis (PMF) patients by genetic variants alone was recently proposed. While non‐inferior to the dynamic international prognostic scoring system (DIPSS), the lack of overlapping prognostic variables between the models leads to increased risk for disagreement between two valid prognostic models and presents a challenging clinical situation. In an external cohort of 266 molecularly annotated myelofibrosis (MF) patients, we demonstrated that the GIPSS model significantly differentiated between four risk groups (low, int‐1, int‐2, high) with median OS that was not reached, not reached, 60.5 and 28.9 months, respectively. High‐risk patients had significantly inferior leukemia‐free survival (LFS) (P < 0.0001). We identified a cohort of prognostically ambiguous patients (n = 39) in which GIPSS and DIPSS models differed by ≥2 risk groups. Among these patients, a similar proportion were up‐staged by DIPSS (n = 19) and GIPSS (n = 20). Patients upstaged by GIPSS (genetically high‐risk) had a trend toward inferior OS compared with patients upstaged by DIPSS (clinically high‐risk) (P = .08) and significantly worse LFS (P = .04). Patients deemed intermediate‐2 and high‐risk by GIPSS who underwent allogeneic transplant had improved OS compared with those that did not (P = .04). GIPSS is a valid disease‐specific prognostic system and outperforms DIPSS in patients where the two models disagree. Additionally, while GIPSS was developed for PMF; the current study shows, however, that the contemporary genetic model performs equally well for both primary and secondary myelofibrosis.
Repellent action and contact toxicity mechanisms of the essential oil extracted from Chinese chive against Plutella xylostella larvae
Authors: Gao Quan , Song Li , Sun Jia , Cao Hai‐Qun , Wang Likun , Lin Huafeng , Tang Feng 
Published: November 2018

AbstractBotanical pesticides play increasingly important roles in the control of agricultural pests. In this study, the insecticidal effects, specifically the repellent action and contact toxicity, of the essential oil extracted from Chinese chive (EOC) against Plutella xylostella larvae were confirmed. The mechanisms of repellent’s action were studied using electroantennograms (EAGs), and the effects on glutathione
S‐transferase (GST), carboxylesterase (CarE), and acetyl cholinesterase were investigated after EOC treatments. The EOC affected the EAG results and inhibited the activities of GST and CarE in treated
P. xylostella larvae, which could explain its insecticidal effects. And, four pyrazines showed greater repellent activities than that of the EOC, which was confirmed as the main active compounds of EOC.
Interleukin‐17A Inhibition Diminishes Inflammation and New Bone Formation in Experimental Spondyloarthritis
Authors: van Tok Melissa N. , van Duivenvoorde Leonie M. , Kramer Ina , Ingold Peter , Pfister Sabina , Roth Lukas , Blijdorp Iris C. , van de Sande Marleen G. H. , Taurog Joel D. , Kolbinger Frank , Baeten Dominique L. 
Published: November 2018

ObjectiveIt remains unclear if and how inflammation and new bone formation in spondyloarthritis (SpA) are coupled. We undertook this study to assess the hypothesis that interleukin‐17A (IL‐17A) is a pivotal driver of both processes.MethodsThe effect of tumor necrosis factor (TNF) and IL‐17A on osteogenesis was tested in an osteoblastic differentiation assay using SpA fibroblast‐like synoviocytes (FLS) differentiated with dexamethasone, β‐glycophosphatase, and ascorbic acid. IL‐17A blockade was performed in HLA–B27/human β2‐microglobulin (hβ2m)–transgenic rats, which served as a model for SpA in both prophylactic and therapeutic settings. Inflammation and new bone formation were evaluated by micro–computed tomography imaging, histologic analysis, and gene expression profiling.ResultsTNF and IL‐17A significantly increased in vitro osteoblastic differentiation. In vivo, prophylactic blockade of IL‐17A significantly delayed spondylitis and arthritis development and decreased arthritis severity. Anti–IL‐17A treatment was also associated with prevention of bone loss and periosteal new bone formation. Therapeutic targeting of IL‐17A after the initial inflammatory insult also significantly reduced axial and peripheral joint inflammation. This treatment was again associated with a marked reduction in spinal and peripheral structural damage, including new bone formation. RNA sequencing of target tissue confirmed that IL‐17A is a key driver of the molecular signature of disease in this model and that therapeutic anti–IL‐17A treatment reversed the inflammatory signature and the selected gene expression related to bone damage.ConclusionBoth prophylactic and therapeutic inhibition of IL‐17A diminished inflammation and new bone formation in HLA‐B27/hβ2m–transgenic rats. Taken together with the ability of IL‐17A to promote osteoblastic differentiation of human SpA FLS, these data suggest a direct link between IL‐17A–driven inflammation and pathologic new bone formation in SpA.
Efficacy and safety of liraglutide added to insulin therapy in elderly patients with type 2 diabetes
Authors: Tonoike Mie , Chujo Daisuke , Noda Mitsuhiko 
Published: November 2018

SummaryIntroductionThe combination of GLP‐1 receptor agonists and insulin is effective in type 2 diabetes (T2D) treatment. However, its longitudinal efficacy and safety in elderly patients have not been established. We evaluated whether liraglutide (Lira) added to insulin therapy safely improved glycaemic control in T2D patients aged >65 years.MethodsTwenty T2D patients receiving insulin were recruited, and Lira was added to their treatment regimen. Before and 6 months after Lira was added, we assessed the metabolic parameters and continuous glucose monitoring (CGM) data.ResultsSix months after Lira was added, the levels of HbA1c and glycated albumin and body weight were significantly improved, despite the daily doses and number of insulin injections per day being reduced. CGM analysis revealed that the SD and AUC of glucose >180 mg/dL were significantly decreased; the proportion of hypoglycaemic events was not increased.ConclusionLira administration safely improved glycaemic control and reduced body weight. Lira added to insulin therapy may improve the quality of life in elderly T2D patients undergoing insulin therapy, especially those requiring social support.
Estimation of magnesium level in type 2 diabetes mellitus and its correlation with HbA1c level
Authors: Saeed Hajar , Haj Safer , Qasim Bayar 
Published: November 2018

SummaryBackgroundType 2 diabetes mellitus (T2DM) is a common disease; the total number of diabetes patients is expected to reach 366 million by 2030. Magnesium has received considerable attention for its potential role in improving insulin sensitivity and preventing diabetes and its cardiovascular complications. Hypomagnesaemia is linked to poor control of type 2 diabetes mellitus, and depletion of serum magnesium occurs exponentially with duration of disease. The aim of this study was to measure serum magnesium level and the correlation of magnesium level with HbA1c in type 2 diabetes mellitus.MethodsIn this cross‐sectional study, we included 100 diabetic patients; blood is taken for measurement of both magnesium and HbA1c levels. Patients with risk factors of magnesium deficiency were not included.ResultsOf the total 100 patients recruited in our study, majority had diabetes for 8.0 years duration. The mean age of the patients was 53.97 ± 10.65 years, most of them were on oral hypoglycaemic agents (84.9%) followed by combination of oral hypoglycaemic agents and insulin (10.5%), and a small percentage were on insulin alone (4.7%). The mean of serum magnesium and HbA1c levels was 1.88 ± 0.25 mg/dL and 8.38% ± 1.53%, respectively. The majority of the patients had a normal level of magnesium (95.0%); however, most of them had uncontrolled blood glucose (82.0%). The study showed that the serum magnesium level and HbA1c are not significantly correlated (P = 0.462).ConclusionNormal magnesium levels were observed in majority of patients, there is no significant correlation between serum magnesium and HbA1c levels in patients with T2DM, but larger‐scale clinical trials are needed in future.
Interrater reliability in visual identification of interictal high‐frequency oscillations on electrocorticography and scalp EEG
Authors: Nariai Hiroki , Wu Joyce Y. , Bernardo Danilo , Fallah Aria , Sankar Raman , Hussain Shaun A. 
Published: November 2018

SummaryHigh‐frequency oscillations (HFOs), including ripples (Rs) and fast ripples (FRs), are promising biomarkers of epileptogenesis, but their clinical utility is limited by the lack of a standardized approach to identification. We set out to determine whether electroencephalographers experienced in HFO analysis can reliably identify and quantify interictal HFOs. Two blinded raters independently reviewed 10 intraoperative electrocorticography (ECoG) samples from epilepsy surgery cases, and 10 scalp EEG samples from epilepsy monitoring unit evaluations. HFOs were visually marked using bandpass filters (R, 80–250 Hz; FR, 250–500 Hz) with a sampling frequency of 2,000 Hz. There was agreement as to the presence or absence of epileptiform discharges (EDs), Rs, and FRs, in 17, 18, and 18 cases, respectively. Interrater reliability (IRR) was favorable with κ = 0.70, 0.80, and 0.80, respectively, and similar for ECoG and scalp electroencephalography (EEG). Furthermore, interclass correlation for rates of Rs (0.99, 95% confidence interval [CI] 0.96–0.99) and FRs (0.77, 95% CI 0.41–0.91) were superior in comparison to EDs (0.37, 95% CI −0.60 to 0.75). Our data suggest that HFO identification and quantification are reliable among experienced electroencephalographers. Our findings support the reliability of utilizing HFO data in both research and clinical arenas.
RGD‐modified dihydrolipoamide dehydrogenase as a molecular bridge for enhancing the adhesion of bone forming cells to titanium dioxide implant surfaces
Authors: Dayan Avraham , Lamed Raphael , Benayahu Dafna , Fleminger Gideon 
Published: November 2018

AbstractTitanium and its alloys are widely used in dental‐ and orthopedic implants, the outer surface of which is often oxidized to titanium dioxide (TiO2). To achieve efficient osseointegration with bone‐forming cells, it is desirable to counter the formation of the soft fibrous tissue around the implant by creating strong and stable interactions between the implant surface and bone‐forming osteoblasts. To address this challenge, a bioactive coating had to be designed. Protein adsorption to TiO2 is well known in the literature, but it is mostly characterized by weak associations, rendering less efficient implant osseointegration. We have previously demonstrated the unique conjugation between the dihydrolipoamide dehydrogenase (DLDH) protein and TiO2 surfaces, based on specific coordinative bonding via Cys–His–Glu–Asp motif residues. To enhance cell binding to DLDH and facilitate osseointegration, DLDH was bioengineered to include Arg–Gly–Asp (RGD) moieties (DLDHRGD). Coating TiO2 disks with DLDHRGD led to improved adherence of integrin‐expressing osteogenic MBA‐15 to the surface of the disks. Following the enhanced adsorption, higher proliferation rates of the adherent cells, as well as faster mineralization were observed, compared to controls. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 545–551, 2019.
Combinational effects of mechanical forces and substrate surface characteristics on esophageal epithelial differentiation
Authors: Wu Yanru , Kang Yun Gyeong , Cho Hana , Kim In Gul , Chung Eun‐Jae , Shin Jung‐Woog 
Published: November 2018

AbstractEven the efficacy of substrate and mechanical stimuli in addition to biochemical cues have been recognized in many studies of stem cell differentiation, few studies have been reported on the differentiation into esophageal epithelial cells. Therefore, the aim of this study was set to propose a method of differentiating stem cells into esophageal epithelial cells according to biochemical reagent concentration, substrate properties, and mechanical forces. After the concentration of all‐trans retinoic acid was determined as 5 μM by a baseline experiment, the degree of differentiation was compared in three different kinds of substrates: cover glass, polyurethane (PU) membrane, and electrospun PU sheet (ePU). Then, on the substrate showing the more positive results, that is, ePU, two types of mechanical forces, intermittent hydrostatic pressure (IHP), and shear stress (SS), were applied individually at different magnitudes for the latter 7 days of an overall incubation period of 14 days. Following various biological assays, the lower IHP (50 mmHg) resulted in greater positive effects than the others. Even with cessation of the mechanical force, the relevant markers were remarkably increased. Although the range of factors regulating differentiation was limited, this study nonetheless demonstrated the combinational effects of mechanical force along with substrate type for the first time in related studies. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 552–560, 2019.
Peptide‐modified methacrylated glycol chitosan hydrogels as a cell‐viability supporting pro‐angiogenic cell delivery platform for human adipose‐derived stem/stromal cells
Authors: Dhillon Jobanpreet , Young Stuart A. , Sherman Stephen E. , Bell Gillian I. , Amsden Brian G. , Hess David A. , Flynn Lauren E. 
Published: November 2018

AbstractCell‐based therapies involving the injection of adipose‐derived stem/stromal cells (ASCs) within rationally designed biomaterials are a promising approach for stimulating angiogenesis. With this focus, the current work explored the effects of incorporating integrin‐binding RGD or IKVAV peptides within in situ‐gelling N‐methacrylate glycol chitosan (MGC) hydrogels on the response of encapsulated human ASCs. Initial studies focused on hydrogel characterization to validate that the MGC, MGC‐RGD, and MGC‐IKVAV hydrogels had similar biomechanical properties. ASC viability following encapsulation and culture under 2% O2 was significantly impaired in the MGC‐IKVAV group relative to the MGC and MGC‐RGD groups. In contrast, sustained viability, along with enhanced cell spreading and metabolic activity were observed in the MGC‐RGD group. Investigation of angiogenic transcription suggested that the incorporation of the peptide groups did not substantially alter the pro‐angiogenic gene expression profile of the encapsulated ASCs after 7 days of culture under 2% O2. Consistent with the in vitro findings, preliminary in vivo characterization following subcutaneous implantation into NOD/SCID mice showed that ASC retention was enhanced in the MGC‐RGD hydrogels relative to the MGC‐IKVAV group at 14 days. Further, the encapsulated ASCs in the MGC and MGC‐RGD groups promoted murine CD31+ endothelial cell recruitment to the peri‐implant region. Overall, the results indicate that the MGC‐RGD and MGC hydrogels are promising platforms for ASC delivery, and suggest that strategies that support long‐term ASC viability can augment in vivo angiogenesis through paracrine mechanisms. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 571–585, 2019.
Surface mineralized hybrid nanofibrous scaffolds based on poly(l‐lactide) and alginate enhances osteogenic differentiation of stem cells
Authors: Ataie Maryam , Shabani Iman , Seyedjafari Ehsan 
Published: November 2018

AbstractSurface mineralized nanofibrous scaffolds hold great potential for bone tissue engineering applications. In this study, a new hybrid nanofibrous scaffold composed of alginate/poly(l‐lactide) nanofibers was fabricated using electrospinning method and then crosslinking process was employed. Hydroxyapatite crystal formation took place using in situ precipitation by immersion of the scaffolds in simulated body fluid solution for 10 days at 37°C. The morphologies of the scaffolds were observed using scanning electron microscope. Hydroxyapatite crystal formation on the surface of nanofibers was confirmed using scanning electron microscopy, energy‐dispersive X‐ray spectroscopy, Fourier transform infrared spectroscopy and X‐ray diffraction analysis. The biocompatibility of the fabricated scaffolds was evaluated using mesenchymal stem cell culture and MTT assay. According to alkaline phosphatase activity and calcium content assays, it was concluded that the osteogenic differentiation of stem cells was considerable on scaffolds containing hydroxyapatite crystals in comparison with other specimens. The results showed biocompatibility of the scaffolds and their support from stem cell adhesion, growth and osteogenic differentiation, so the hydroxyapatite‐coated poly(l‐lactide)/alginate hybrid nanofibrous scaffolds hold suitable characteristics for bone tissue engineering applications. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 586–596, 2019.
miR‐124‐3p attenuates neuropathic pain induced by chronic sciatic nerve injury in rats via targeting EZH2
Authors: Zhang Yang , Liu Hai‐Lin , An Li‐Jun , Li Lin , Wei Meng , Ge Dong‐Jian , Su Zhen 
Published: November 2018

AbstractEmerging evidence has suggested that microRNAs play a critical role in neuropathic pain development. However, the biological role of miRNAs in regulating neuropathic pain remains barely known. In our present study, we found that miR‐124‐3p was significantly downregulated in rats after chronic sciatic nerve injury (CCI). In addition, it was showed that overexpression of miR‐124‐3p obviously repressed mechanical allodynia and heat hyperalgesia. Meanwhile, it has been reported that neuroinflammation can contribute a lot to neuropathic pain progression. Here, we found that inflammatory cytokine (IL‐6, IL‐1β, and TNF‐⍺) protein expression in rats after CCI greatly increased and miR‐124‐3p mimics depressed inflammation cytokine levels. Consistently, miR‐124‐3p alleviated inflammation production in lipopolysaccharide‐incubated spinal microglial cells. Bioinformatics analysis revealed that EZH2 acted as a direct target of miR‐124‐3p, which participated in the miR‐124‐3p‐modulated effects on neuropathic pain development and neuroinflammation. We observed that miR‐124‐3p was able to promote neuroinflammation and neuropathic pain through targeting EZH2. The direct correlation between them was validated in our current study using dual‐luciferase reporter assays. Subsequently, it was manifested that EZH2 abrogated the inhibitory role of miR‐124‐3p on neuropathic pain progression in CCI rats. Taken these together, our findings highlighted a novel contribution of miR‐124‐3p to neuropathic pain and indicated the possibilities for developing novel therapeutic options for neuropathic pain.