bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic evaluation of Cryptomeria japonica breeding materials for male-sterile trees
Authors: Tadama Takumi , Totsuka Satoko , Iwai Junji , Uchiyama Kentaro , Hasegawa Yoichi , Moriguchi Yoshinari 
Published: June 2019

Abstract
Pyramiding of male-sterile genes in Cryptomeria japonica is currently being carried out in Niigata prefecture, Japan. This is the first attempt to apply pyramid breeding to forest trees. As the breeding materials for male sterility are limited, special attention must be given to increased genetic relatedness in the process of pyramid breeding to avoid the effects of inbreeding depression as much as possible. In this study, we estimated genetic relatedness based on 246 genome-wide SNP markers for male-sterile individuals in Niigata Prefecture (n = 6) and individuals doubly heterozygous for two male-sterile genes (hereafter referred to as “double-hetero”) produced by marker- assisted selection (n = 124). The pairwise relatedness estimates between male-sterile individuals selected from the same area in Niigata Prefecture were low (−0.01 ± 0.08, mean ± standard deviation), suggesting that there will be almost no negative effects even if the F1 of these male-sterile individuals is used for artificial crossing. On the other hand, the pairwise relatedness between double-hetero individuals in this study was higher than the theoretical relatedness values, as individuals with the slightly higher relatedness were used as parents in artificial crossings. However, there was a large variance in pairwise relatedness for double-hetero individuals. This result suggested that it may be possible to avoid the adverse effects of inbreeding depression by using a pair of double-heteros with lower relatedness for artificial crossing, when we produce a double-homo using the limited breeding materials of male- sterile individuals. It will also be important to continue additional selection of new breeding material for male sterility.
Genetic control of silvicultural traits in Balfourodendron riedelianum (ENGL.) ENGL.
Authors: Aguiar B.I. , Freitas M.L.M. , Tavares Y.R. , Tambarussi E.V. , Zanatto B. , Gandara F.B. , Paludeto J.G.Z. , Silva D.Y.B.O. , Silva J.R. , Moraes M.L.T. , Longui E.L. , Zanata M. , Sebbenn A.M. 
Published: June 2019

Abstract
The Atlantic Forest has very high levels of biodiversity and is considered one of the most important and threatened biomes in the world. Balfourodendron riedelianum is one of the forest’s characteristic tree species that is currently under considered endangered due to timber extraction and forest fragmentation. With the goal of generating information that may contribute to the genetic conservation of B. riedelianum, genetic parameters are estimated for quantitative traits important for silviculture in a provenance and progeny test located in the Experimental Station of Luiz Antônio, São Paulo State, Brazil. The test was established in 1986 and consists of three provenances, from which open-pollinated seeds from 19 seed trees were collected. The following traits were evaluated at 32 years of age: diameter at breast height (DBH), total height (H), stem height (SH), stem straightness (SS), and forking (FOR). The estimates were carried out using the REML/BLUP method. Significant genetic variation among progenies was detected for all traits (except SH) and between provenances for DBH. The coefficient of individual genetic variation (CV

gi
) ranged from 2.5 to 9.5 %. The mean heritability among progeny (

h

f
2

$h_f^2$

) was substantial for DBH (0.44) and FOR (0.36), enabling the selection of families with the highest DBH and lowest FOR for population improvement. Genotypic and phenotypic correlations among traits were also found. We conclude that there is genetic variability in the population that can be exploited in future breeding programs and for the genetic conservation of the species.
Comparison of DNA Yield from Different Plant Materials of Plumeria sp. (Apocynaceae)
Authors: Martida Vanesa , Pharmawati Made 
Published: June 2019

DNA extraction that gives good quantity and quality DNA is a basic step that must be completed for molecular studies, especially in DNA fingerprint imaging. The aim of this research was to find out the better quality and quantity of DNA extracted from different plant materials of frangipani cultivars (Plumeria sp.). Leaves and flowers were collected from Taman Jepun, Denpasar Bali. Fresh young leaves and flowers were used as plant materials as well as dried leaves (silica gel dried leaves) of Plumeria sp. This research used CTAB buffer with modification as lysis buffer. Purification techique used NucleoSpin® Gel and PCR Clean Up Kit. The results showed that the colour of DNA solution from fresh material was clear and the quantities of DNA from young fresh leaves were between 70-300 ng/µl. The DNA colour solution from flowers was also transparent with concentration between 0-40 ng/µl. DNA isolated from dry material resulted in brown solution with DNA quantity between 30-100 ng/µl and need to be purified to obtain clear DNA solution.
DISTRIBUSI SPASIO -TEMPORAL NITROGEN DI DAERAH TANGKAPAN AIR DANAU TONDANO PROVINSI SULAWESI UTARA
Authors: Wantasen Sofia , Luntungan Joudie N. 
Published: June 2019

The spatio temporal distribution of nitrogen in catchment area of Lake Tondano wasimportant to investigate because agricultural residue comes from the catchment potentiallydecreased water quality in Lake Tondano. The purpose of this study was to examine thedistribution of nitrogen spatially and temporally in the catchment area of Lake Tondano.The study was conducted in the field and laboratory. Water quality sampling conducted byusing composite sampling on the rivers, lake Tondano and outlet of irrigation channels.The data collected was the total nitrogen, nitrate, ammonia in 2001, 2008 and 2014. Datawere analyzed using Graph Method and Spatial Analysis. The results showed that the distribution of total nitrogen that has a high concentrationfound in the outlet streams and irrigation canals outlet. High nitrate accumulates in theoutlet of Lake Tondano. Nitrate concentration can be divided into 9 (nine) classes, namelyconcentration of 0.100 to 0.211 mg / l; 0.211 to 0.322 mg / l; 0.322 to 0.433 mg / l; 0.433 to0.544 mg / l; From 0.544 to 0.655 mg / l; From 0.655 to 0.766 mg / l; 0.766 to 0.877 mg / l;0.877 to 0.988 mg / l; and 0,988- 1,100 mg / l. Ammonia, spatially spread evenly on thesurface of Lake Tondano. Ammonia concentration can be divided into 9 (nine) class is aclass that has a concentration of 0.000 to 0.064 mg / l; From 0.064 to 0.129 mg / l; 0.129 to0.193 mg / l; From 0.193 to 0.258 mg / l; 0.258 to 0.322 mg / l; 0.322 to 0.387 mg / l; From0.387 to 0.451 mg / l; 0.451 to 0.516 mg / l; and from 0.516 to 0.580 mg / l. Concentrationsof nitrate and ammonia fluctuated from year to year of observation. This was influenced byfertilization in rice fields.
PENGELOLAAN CULTURAL LANDSCAPE PURA BATUKARU SEBAGAI DAYA TARIK EKOWISATA
Authors: Wardi I Nyoman , Srijaya I Wayan 
Published: June 2019

The research aims to uncover the potential and management Cultural Landscape PuraBatukaru (Batukaru Temple) as the appeal of ecotourism in Tabanan and Bali in general.The study was conducted by the method of data collection and analysis. The data collectionis done by observation, interview (dept-interview), and literature study. The data wereanalyzed descriptively qualitative.The study showed that Pura Batukaru reserve that has been set by UNESCO as part ofthe World Cultural Landscape of Bali can be classified into Associative Cultural Landscape(Associative Cultural Landscapes). More details, namely the associative cultural landscapenuances of local culture (Bali). Cultural landscape nuances of local culture Bali includesthree main components of the environment (human-nature-god) in the relationship andinteraction interdependence known as Tri Hita Karana.Based on the names of palinggih (shrine), namely Pucak Kedaton / Sang HyangTumuwuh, and figures of other gods), stone shrines (natural stone) and communities tomanage these (community Bali Age / Bali Mula and Jero Kubayan), historically likelyHeritage Pura Batukaru already exist on prehistoric times (Neolithic-Megalithik). Then,when the kingdom’s political system (mornachi) introduced by Hindu culture, figures ofkings (royalty) was also honored at the site by building shrines as media for ancestralworship.Cultural Landscape of Pura Batukaru can be classified into Associative CulturalLandscape (Associative Cultural Landscapes), namely the associative cultural landscapenuances of local culture (Bali). Cultural landscape nuances of local culture Bali includesthree main components of the environment (human-nature-god) in the relationship andinteraction interdependence known as Tri Hita Karana.Heritage of Pura Batukaru with its jajar-kumiri network (pecan row) and relationswith Ulun Danu Tamblingan form a sacred area (sacred cultural landscape of Batukaru)and the rituals and myths that exist in it has important value in maintaining and preservingthe natural environment for a variety of purposes and interests of the community andgovernment in development, especially the development of ecotourism in a sustainablemanner in Tabanan and Bali in general.
Validasi Nilai Erosivitas Hujan Dari Data Penginderaan Jauh TRMM 3B42 Di Bali Selatan
Authors: Adnyana I Wayan Sandi , As-syakur Abd. Rahman 
Published: June 2019

Rainfall erosivity is a measure for the erosive force of rainfall. Rainfall kinetic energydetermines the erosivity and is in turn greatly dependent on rainfall intensity. Research hasbeen conducted to validate monthly rainfall erosivity derived from the Tropical Rainfall MeasuringMission (TRMM) Multisatellite Precipitation Analysis(TMPA)3B43 version 7 usingraingauge data analysis from 2003 to 2012. Rain gauge located in the south Bali regions wereemployedto monitor erosivity value from two different methods that are base on Bols (1978)andAbdurachman(1989). Therelationship of erosivity and their other factor from TRMM3B43andrain gauge data statistical analysis measures consisted of the linear correlation coefficient,themean bias error (MBE), and the root mean square error (RMSE). Data validation wasconductedwith point-by-point analysis. The results of these analyses indicate that satellitedatahave lower values than the gauge estimation values. The point-by-point analysis indicatedsatellite data values of high to very high correlation, while values of MBE and RMSEtendedto indicate underestimations with high square errors. Moreover,monthly rainfall erosivityderived from TRMM give high correlation from both methods, with has high bias androot-mean-squareerror. In general, the data from TRMM3B43 version 7 are potentially usabletoreplace rain gauge data based on erosivity estimation, but after inconsistencies and errorsaretaken into account.
MODEL PENANGGULANGAN SEDIMENTASI DANAU BERBASIS MASYARAKAT DI PULAU BALI
Authors: Nada I Made , Suryatmaja Ida Bagus , Wiswasta I Gusti Ngurah Alit 
Published: June 2019

Countermeasures of community-based lake sediments are directed at maintaining the dynamic balance of Lake Batur, Beratan Lake, Lake Buyan and Lake Tamblingan. Sedimentation process of this lake is triggered by the pattern of management of agricultural land on the edge of the lake is less attention to aspects of soil and water conservation. The purpose of this research is; (1) to obtain a community-based sedimentation prevention model, (2) Mapping Land management on the edge of the lake, (3) erosion control strategy at the edge of the lake. This research is field experiment and surpei research, sampling is done by purposive sampling at Batur Lake 20 samples, Beratan Lake 16 samples, Lake Buyan 16 samples and Danau Tamblingan 12 Sampel. The socio-economic support data of the community was determined by 180 respondents in cluster sampling. Interviews and questionnaires are closed questions on a conventional scale. Characteristics of the lake were analyzed descriptive statistics using SPSS analysis tool. Land mapping is done by GPS method with GIS device. Predict erosion using the Universal Soil Loss Equation (USLE) Model while for sedimentation using the Stanford Sediment model. Data analysis to find out the model of development of sedimentation rate in lake due to erosion that happened, done by non linear regreresi analysis. The results of the analysis are tested on both sides with 95% confidence level on sediment data that is modeled whether or not significant effect
Coral Reef and Shallow Water Benthic Identification Using Landsat 7 ETM+ Satellite Data in Nusa Penida District
Authors: Fahlevi Arief Reza , Osawa Takahiro , Arthana I Wayan 
Published: June 2019

This study aims to investigate the feasibility of Landsat 7 ETM+ to identify coral reefs and shallow water benthic at Nusa Penida district in 2009 and 2012, comparison with the Reef Health Monitoring (RHM) results conducted by the Coral Triangle Center (CTC) using the Point Intercept Transect (PIT) method in the same periods. This study also aims to determine the changes of coral reefs and shallow water benthic cover during this period and the distribution at Nusa Penida districk. Shallow water benthic classification refers to English et al. (1997), with a modified by the addition of vegetation classes (seagrass and seaweed). The classification result using confusion matrix between the Reef Health Monitoring (RHM) with the classification of satellite image in 2009 obtained an accuracy rate of 65.85% with a kappa coefficient value of 0.525, while in 2012 the accuracy value obtained of 74.81% with kappa coefficient value of 0.650, which shows the results of that classification of satellite images of Landsat 7 ETM+ with the results of in-situ research is in a moderate level.
Estimation of Carbon Sequestration in Tropical Peat Swamp Forest in Central Kalimantan Using Satellite Based on Primary Productivity
Authors: Agustin Dewa Ayu Mery , Osawa Takahiro , Ardhana I Putu Gede 
Published: June 2019

One of approach that can be used to estimate the carbon sequestration by vegetation is to calculate the Gross Primary Productivity (GPP) in Central Kalimantan. GPP is total carbon that can be absorbed by vegetation to be used in the process of photosynthesis. The purpose of this study is to estimate the value of GPP using Vegetation Photosynthesis and Respiration Model (VPRM) and analyze the data comparison between GPP value data derived from flux tower and GPP value data from MODIS data. The field data from flux tower was taken by Hirano et al. (2007) from January 1, 2004 to December 31, 2005. The MODIS data is used MODIS Surface Reflectance Level 3 data year 2004 to 2005. According to the result of this study, the maximum GPP value year 2004 and 2005 showed 302.365 gC m-2 per month (February 2004) and 366.841 gC m-2 per month (June 2005). The minimum GPP value year 2004 and 2005 was 166.003 gC m-2 per month (November 2004) and 187.663 gC m-2 per month (March 2005). The total value of GPP in year 2004 was 1,134.231 gC m-2 yr-1 and in year 2005 the value was 1,109.001 gC m-2 yr-1. The correlation coefficient between GPP value from flux tower and GPP value from MODIS – VPRM showed in dry season, r = 0.766 and in rainy season, r = 0,839.
Study of Land Use Change from 1997 To 2014 Using Landsat Data In Bangli Regency
Authors: Naningsi Nuranita , Osawa Takahiro , Merit I Nyoman 
Published: June 2019

Bangli Regency is one of Regency in the Bali Province. The total area of Bangli Regency is 52,081 hectares (9.24%) of total area of Bali Province (563,666 ha). The Growth and the development of the region Bangli Regency the positive impacts on the economy of the community, and the negative impacts on the environment. Land use change is one of the negative issue of development Bangli Regency. This study conduted the calculation of land use change from 1997 to 2014 using Landsat data in Bangli Regency. Landsat 5 TM, Landsat 7 ETM+ and Landsat 8 OLI/TIRS imageries were used to determine the land use map based, on using supervised classification method. The field data set the nine classes were classtuded based, on the classification were fresh water, bare land, forest, residential, bushes, irrigated paddy field, non irrigated paddy field, dry land and plantation. There results showed in land use changes from 1997 to 2014 that plantation increased (19,486.33 ha (36.89%)), and residential increased (1,872.00 ha (3.47%)), there is also a vast to reduction in dry land (-10,868.90 ha (-21.21%)), forest (-6,333.34 ha (-12.24%)), irrigated paddy field (-1,619.50 ha (-3.17%)), bushes (-1,637.30 ha (-3.27%)), bare land (-63.00 ha (-0.17%)), non irrigated paddy field (-113.59 ha ( -0.26%)) and fresh water (-2.70 ha (-0.05%). The results accuracy rate was 89.45%. Anslyse of land use showed that the significant decrease of plantation area in Bangli Regency hill due to rapid development of infrastrusture of tourism and extensive residential area has increased particularly in sub district of the Kintamani District.
Validitas Rasio Neutrofil Limfosit pada Apendisitis Komplikata di RSUP Sanglah Denpasar
Authors: Christian Dewi Prima , Suwedagatha I Gede , Golden Nyoman , Wiargitha I Ketut 
Published: June 2019

Tujuan: untuk mengetahui validitas rasio neutrofil limfosit (RNL) pada apendisitis komplikata. Metode: penelitian dilakukan secara observasional analitik dengan menggunakan desain studi kohort dengan mengambil sampel penderita apendisitis akut yang menjalani apendisektomi di RSUP Sanglah Denpasar, periode Oktober-Desember 2015. Data dikelompokkan menjadi dua kelompok, yaitu RNL dengan cut of point >5 dan RNL dengan cut of point ?5 dan kemudian disesuaikan dengan temuan pemeriksaan histopatologi anatomi sebagai standar baku emas, komplikata dan non-komplikata. Data tersebut kemudian dianalisis dengan analisis statistik deskriptif, analisis kurva ROC, dan uji diagnostik. Hasil: pada penelitian ini diperoleh 62 sampel, dengan median umur 23 tahun, 32 orang penderita laki-laki, 30 orang penderita perempuan, 28 apendisitis non-komplikata, dan 34 apendisitis komplikata. Dari area under curve ROC 0,6229 dengan 95% CI didapatkan cut of point RNL >5 pada apendisitis komplikata, RNL ?5 pada apendisitis non-komplikata. Uji diagnostik didapatkan nilai sensitivitas 85,3%, spesifisitas 39,3%, dan tingkat akurasi 64,5%. Simpulan: RNL merupakan tolak ukur sederhana yang lebih baik untuk meramalkan apendisitis akut dibandingkan dengan penilaian Alvarado Score dan USG abdomen serta valid untuk membedakan apendisitis komplikata dan non-komplikata melalui cut of point RNL.
Penatalaksanaan Batu Ginjal dengan Stone Burden Lebih dari Dua Sentimeter di Rumah Sakit Pusat Angkatan Darat Gatot Subroto Tahun 2011-2014
Authors: Aslim Octoveryal , Utomo Nugroho Budi , Prasidja Nindra , Prasetyo Robertus Bebet 
Published: June 2019

Tujuan: untuk mengetahui perbandingan lama operasi, lama perawatan paska operasi, jumlah perdarahan, komplikasi, angka bebas batu, dan hubungan antara komponen tersebut pada operasi percutaneous nephrolithotomy (PCNL) dan operasi terbuka baik, pyelolithotomy maupun extended pyelolithotomy sebagai penatalaksanaan batu ginjal dengan stone burden lebih dari 2 cm. Metode: pengumpulan data dilakukan secara retrospektif yang diambil dari rekam medis pasien batu ginjal yang menjalani PCNL dan operasi terbuka di Rumah Sakit Pusat Angkatan Darat (RSPAD) Gatot Soebroto sepanjang tahun 2011 hingga tahun 2014. Hasil: dari 116 pasien dengan usia antara 22-73 tahun, mayoritas laki-laki, didapatkan hasil perbedaan yang bermakna secara statistik pada lama operasi (p=0,001), lama rawat paska operasi (p=0,011), dan komplikasi demam paska operasi (p=0,048), antara PCNL dan operasi terbuka. Sedangkan untuk parameter angka bebas batu dan jumlah perdarahan, tidak didapatkan perbedaan yang bermakna secara statistik (p=0,245 dan p=0,154). Pada kelompok PCNL dan operasi terbuka, terdapat hubungan yang bermakna pada lama operasi dengan stone burden (p=0,004 dan p=0,02) maupun letak batu (p<0,001 dan p=0,011). PCNL memerlukan lama operasi dan lama rawat paska operasi yang lebih singkat, serta komplikasi demam paska operasi yang lebih sedikit, dibandingkan operasi terbuka. Namun demikian, untuk angka bebas batu dan jumlah perdarahan, tidak berbeda bermakna pada kedua kelompok. Terdapat hubungan yang bermakna secara statistik pada lama operasi dengan stone burden dan letak batu pada kedua kelompok. Simpulan: PCNL merupakan prosedur minimal invasif yang efektif dan aman dalam tatalaksana batu ginjal lebih besar dari 2 cm atau batu ginjal kompleks.
Faktor Risiko Terjadinya Batu Empedu di RSUP Dr. Wahidin Sudirohusodo Makassar
Authors: Sueta Made Agus Dwianthara , Warsinggih Warsinggih 
Published: June 2019

Tujuan: untuk mengetahui apakah terdapat hubungan beberapa faktor seperti jenis kelamin, usia, diabetes, obesitas, dan hiperlipidemia, sebagai penyebab munculnya batu empedu di RSUP Dr. Wahidin Sudirohusodo. Metode: penelitian ini dilakukan dengan pengambilan sampel secara retrospektif pada pasien yang dirawat diruang bedah di Rumah Sakit Wahidin Sudirohusodo Makassar bulan Januari sampai dengan Desember 2013, sampel diambil secara konsekutif berurutan sebesar jumlah sampel yang dibutuhkan. Hasil: sampel yang masuk kriteria inklusi dari penelitian ini sebanyak 196 orang. Dari 196 orang 114 (58,5%) orang dengan batu empedu, 101 perempuan (51,5%) dan 95 laki-laki (48,5%). Terdapat 86 (75,4%) penderita dengan batu empedu yang berusia di bawah 40 tahun dan 28 (24,6%) penderita berusia lebih dari 40 tahun. Berdasarkan jenis kelamin 26 (22,8%) penderita laki-laki dengan batu empedu dan 88 (77,2%) perempuan dengan batu empedu. Penderita DM dengan batu empedu sebanyak 103 (90,4%), pasien dengan obesitasyang menderita batu empedu sejumlah 97 (85,1%), 88 (77,2%) penderita dengan kadar trigliserida yang meningkat dengan batu empedu, dan 95 (83,3 %) pasien dengan kadar kolesterol yang meningkat mengalami batu empedu. Simpulan: jenis kelamin perempuan, usia di bawah 40 tahun, penderita diabetes, obesitas dan hiperlipidemia merupakan faktor risiko menimbulkan batu empedu.
Dosis Efektif Enoxaparin dalam Mencegah Terjadinya Trombosis pada Anastomosis Arteri Femoralis Tikus
Authors: Adnyana I Made Suka , Saputro Iswinarno Doso 
Published: June 2019

Tujuan: untuk mengetahui dosis efektif enoxaparin dalam mencegah terjadinya trombosis pada anastomosis mikrovaskular. Metode: penelitian ini bersifat eksperimental dengan rancangan the randomized post test only control group design. Terdapat 33 tikus jantan Rattus norvegicus Wistar strain yang dikelompokkan menjadi tiga perlakuan yaitu perlakuan A (enoxaparin 0,75 mg/kg), B (enoxaparin 1 mg/kg), dan C (kontrol). Tuck model anastomosis dilakukan pada arteri femoralis, kemudian luas trombus yang terjadi pada pembuluh darah dibandingkan dengan diameter lumen pembuluh darah diukur dengan graticule lens dan dinyatakan dalam persen. Hasil: trombus terbentuk pada semua subyek penelitian baik pada kelompok perlakuan maupun kontrol. Rerata persentase luas trombus pada kelompok enoxaparin 0,75 mg/kg adalah 24,3%, enoxaparin 1 mg/kg sebesar 19,8% dan kelompok NaCl 0,9% sebesar 79,4%. Terdapat perbedaan antara perlakuan pemberian enoxaparin dosis 0,75 mg/kg dan 1 mg/kg dengan kontrol, namun tidak ada perbedaan bermakna rerata persentase luas trombus diantara kelompok enoxaparin dosis 0,75 mg/kg dan dosis 1 mg/kg (p=0,624). Perlu dilakukan penelitian secara klinis guna melihat efektivitas enoxaparin dalam meningkatkan patensi anastomosis pada free flap maupun replantasi. Simpulan: pemberian enoxaparin dosis 0,75 mg/kg dan enoxaparin dosis 1 mg/kg secara subkutan efektif mengurangi persentase luas trombus pada anastomosis arteri femoralis tikus. Tidak terdapat perbedaan efektivitas yang bermakna dalam mengurangi persentase luas trombus pada anastomosis arteri femoralis tikus setelah diberikan dosis enoxaparin 0,75 mg/kg dan 1 mg/kg secara subkutan.