bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biochemical principles of miRNA targeting in flies
Authors: Vega-Badillo Joel , Zamore Phillip D. , Jouravleva Karina 
Published: November 2024

SummaryMicroRNAs-direct Argonaute proteins to repress complementary target mRNAs via mRNA degradation or translational inhibition. While mammalian miRNA targeting has been well studied, the principles by whichDrosophilamiRNAs bind their target RNAs remain to be fully characterized. Here, we use RNA Bind-n-Seq to systematically identify binding sites and measure their affinities for four highly expressedDrosophilamiRNAs. Our results reveal a narrower range of binding site diversity in flies compared to mammals, with fly miRNAs favoring canonical seed-matched sites and exhibiting limited tolerance for imperfections within these sites. We also identified non-canonical site types, including nucleation-bulged and 3′-only sites, whose binding affinities are comparable to canonical sites. These findings establish a foundation for future computational models ofDrosophilamiRNA targeting, enabling predictions of regulatory outcomes in response to cellular signals, and advancing our understanding of miRNA- mediated regulation in flies.
A definition of the body of knowledge for real estate: reconceptualizing the discipline and delineating the jurisdictions of its practice
Authors: K'Akumu Owiti A. 
Published: November 2024

PurposeFollowing calls at different times in the United States for the definition of the body of knowledge (BOK) of real estate discipline, this briefing sets out to define the intellectual boundaries of real estate and applies the definitional concept to map out the scopes of real estate professions and real estate valuation vocationDesign/methodology/approachThe study essentially uses a literature review to draw out definitional issues that have been tackled before. It reviews calls that have been made in the past for a definition of the BOK of the real estate discipline. Further, it reviews past attempts at definition to reveal why they failed at the definition project.FindingsThe study found three key suggestions made in the past: that there is a need to define real estate’s BOK, that there is a need to use a “central structure” to define the BOK of real estate, and that definition of the BOK of real estate should rely on the activity nodes that constitute real estate. The study has relied on these three key ideas to define real estate as the science of value creation, distribution and sustenance in built space.Practical implicationsThe definition of the BOK will help in curriculum development for real estate education. It will help faculties and departments to single out what fits into the real estate curriculum and what does not fit in.Originality/valueA definition of the BOK for real estate: reconceptualizing the discipline and delineating jurisdictional boundaries of its practice.
The emergence of collective moral elevation through multilevel individual and organizational processes
Authors: Marasli Furkan , Golgeci Ismail , Akgun Ali Akber , Müceldili Büşra , Arslan Ahmad 
Published: November 2024

PurposeThis paper aims to extend the concept of moral elevation from the individual level to the team/group level by introducing the notion of collective moral elevation (CME) and elucidating its emergence.Design/methodology/approachDrawing on social exchange theory and social capital arguments, this paper presents a conceptual framework that outlines the mechanisms through which moral norms, organizational symbols, social cohesion and repeated interactions facilitate the transition from individual internalization of norms to collective aggregation of moral elevation.FindingsThe proposed framework emphasizes the processual nature of CME, highlighting the importance of understanding sequences of events rather than merely examining static relationships between variables.Originality/valueThis paper is one of the pioneering academic works to offer a novel perspective on moral elevation, exploring its manifestation at the collective level and elucidating the dynamics of its emergence and evolution within teams and groups in organizational settings. Our proposed framework explicates how moral norms, organizational symbols, social cohesion, emulation and their iterations allow individuals to transition from individual internalization of norms to collective aggregation of moral elevation.
The “insider” ethnographic diagnostic radiographer thinking like “an outsider”
Authors: Strudwick Ruth Mary 
Published: November 2024

PurposeThe purpose of this paper is to explore how the ethnographic researcher navigates their insider–outsider status and provides a methodological contribution to this important aspect of ethnographic research; this will be framed from the researcher’s perspective using a semi-autoethnographic approach. The ethnographic study being reflected upon explored the culture in a Diagnostic Imaging Department (DID), looking at how radiographers work and what the issues were within their working environment. The original study was carried out within one DID in a District General Hospital in the East of England (Strudwick, 2011).Design/methodology/approachIn the original study, the researcher used ethnography to study the culture in a DID. Observation was carried out for a four-month period. Field notes were recorded and used to formulate topics for the interviews that were to follow. After the observation, the researcher conducted semi-structured interviews with key informants from the DID. Ten key informants were purposefully sampled from the DID to provide a cross-section of opinion from the staff. The data collected were analysed to identify key themes. This paper reflects on the data from the original study to explore the tensions between the insider and outsider researcher role and how this contributes to the way the ethnographic researcher views the environment, reports on their findings and how they feel about the data from their own perspective.FindingsEthnographers carrying out research in their own area of practice need to try to think like an outsider in order to see the environment with a sense of strangeness but also try to make sense of what the participants are thinking and doing. There is a tension between becoming part of the group in order to understand it and looking at the environment as an outsider in order to make a note of what is happening. Findings from the original ethnographic study will be used to illustrate this point and will be used to reflect on the feelings of the researcher, considering her insider and outsider status.Research limitations/implicationsThis study was carried out in one diagnostic imaging department in the east of England.Originality/valueThe author, who is a diagnostic radiographer and radiography educator reflects on how she managed the insider–outsider tension during her ethnographic observation and after the event when reflecting on the data from the original study.
FinTech Adoption and Corporate Greenwashing: A Technology Affordance Perspective
Authors: Sun Zhe , Liu Lei , Johan Sofia , Zhao Liang 
Published: November 2024

AbstractAffordance theory suggests that technology offers certain opportunities or ‘affordances’ that can be exploited by users. In this context, we are exploring how FinTech adoption provides opportunities to address corporate greenwashing. Drawing on an affordance perspective, we assert that FinTech adoption effectively inhibits corporate greenwashing behaviour, primarily through facilitating green innovation and improving managerial efficiency. We also contend that the impact of FinTech adoption on reducing greenwashing behaviour is not uniform across all industries. It is more pronounced in heavy‐polluting industries, indicating that FinTech has a greater effect in encouraging accurate disclosure of environmental information in environmentally sensitive sectors. Conversely, its impact is weaker in high‐tech industries, which might already have strong environmental commitments. The findings contribute to the literature on sustainability, FinTech and governance.
Anthracological analysis of wooden shaft remains from the Roman‐period sacrificial lake of Nidajno, Czaszkowo 1 site, north‐eastern Poland
Authors: Rzeszotarska‐Nowakiewicz Aleksandra , Kontny Bartosz , Nowakiewicz Tomasz , Cywa Katarzyna 
Published: November 2024

AbstractHere, we report on an archaeobotanical study of charred wood remains from eight iron spearhead sockets from a sacrificial aquatic site at Nidajno/Czaszkowo 1, Mazurian Lakeland, NE Poland (c. second–fifth c. CE), in search of botanical evidence for the selection of wood for manufacture of wooden shafts used with iron spearheads. The samples were analysed using a Nicon Eclipse ME600 metallographic microscope. The results show significant botanical data for Fraxinus excelsior L. and Tillia sp.The results of this research illustrate the material standard of the finds from a unique sacrificial site of the Scandinavian ‘sacrificial bog’ type, such as the sacrificial site at Nidajno/Czaszkowo 1—one of the most scientifically prospective in the entire zone of the North European Barbaricum. They also reveal a clear preference in the way spears and javelins were made, linked to the use of ash wood for this purpose. This choice is rational, justified by the technological and operational properties of this type of wood, but it also has connotations from the world of symbolic religious imagery.
A Global Synthesis of Environmental Enrichment Effect on Fish Stress
Authors: Zhang Zonghang , He Yijie , Wang Jiujiang , Zheng Yating , Mo Jiezhang , Zhang Xiumei , Liu Wenhua 
Published: November 2024

ABSTRACTThe stress‐coping ability (SCA) is one of the core aspects of fish welfare and is of vital importance for fish production in the aquaculture industry and for fish fitness in hatchery release. Environmental enrichment (EE), a method of introducing external stimuli into the husbandry environment, has been recently proposed to improve fish SCA, but the present experimental evidence is mixed, and the reasons for these discrepancies are unclear. Here, we conducted a global meta‐analysis using a data set that consists of 1786 cases from 107 studies across 42 fish species to solve this problem. Overall, we found that enriched fish had significantly higher SCA than control fish, reflected in either basal stress levels or stress responses. Meta‐regression analyses showed that specific subgroups of enrichment type, fish developmental stage, stress category, stress duration, stress place, sample tissue and indicator system showed significant positive EE effects on SCA. Multi‐model inference indicated that the indicator system, fish developmental stage, stress characteristic and enrichment mode are important drivers for the high heterogeneity among effect sizes. These results highlight the importance of introducing EE into the rearing systems, which will not only increase the welfare of aquaculture fish but also improve the ecological adaptability of released fish. The comprehensive knowledge obtained in this analysis will provide insights into fish ontogenetic plasticity and its responses to EE and have important implications for improving the production cycle in fish aquaculture and fisheries conservation.
Solving the minimum labeling global cut problem by mathematical programming
Authors: Koehler Victor J. de Sousa , Gouveia Thiago , Sousa Filho Gilberto Farias de , Ochi Luiz Satoru , Michelon Philippe , Gueye Serigne , Cabral Lucidio A. F. 
Published: November 2024

AbstractAn edge‐labeled graph (ELG) is a graph such that is the set of vertices, is the set of edges, is the set of labels (colors), and each edge has a label associated. Given an ELG , the goal of the minimum labeling global cut problem (MLGCP) is to find a subset such that the removal of all edges with labels in disconnects and is minimum. This work proposes three new mathematical formulations for the MLGCP, namely PART, VC, and TE as well as branch‐and‐cut algorithms to solve them. Additionally, a theoretical study was carried out on the MLGCP input graph, leading to the concept of chromatic closure, used in preprocessing algorithms for this model PART. Finally, a comprehensive polyhedral investigation of the model is performed. The computational experiments showed that the model, adopting the chromatic closure concept and its branch‐and‐cut algorithm, can solve small to average‐sized instances in reasonable times.
Immunosuppressive SOX9‐AS1 Resists Triple‐Negative Breast Cancer Senescence Via Regulating Wnt Signalling Pathway
Authors: Ye Xuan , Cen Yi , Li Quan , Zhang Yuan‐Ping , Li Qian , Li Jie 
Published: November 2024

ABSTRACTLong noncoding RNAs (lncRNAs) are involved in the regulation of triple‐negative breast cancer (TNBC) senescence, while pro‐carcinogenic lncRNAs resist senescence onset leading to the failure of therapy‐induced senescence (TIS) strategy, urgently identifying the key senescence‐related lncRNAs (SRlncRNAs). We mined seven SRlncRNAs (SOX9‐AS1, LINC01152, AC005152.3, RP11‐161 M6.2, RP5‐968 J1.1, RP11‐351 J23.1 and RP11‐666A20.3) by bioinformatics, of which SOX9‐AS1 was reported to be pro‐carcinogenic. In vitro experiments revealed the highest expression of SOX9‐AS1 in MDA‐MD‐231 cells. SOX9‐AS1 knockdown inhibited cell growth (proliferation, cycle and apoptosis) and malignant phenotypes (migration and invasion), while SOX9‐AS1 overexpression rescued these effects. Additionally, SOX9‐AS1 knockdown facilitated tamoxifen‐induced cellular senescence and the transcription of senescence‐associated secretory phenotype (SASP) factors (IL‐1α, IL‐1β, IL‐6 and IL‐8) mechanistically by resisting senescence‐induced Wnt signal (GSK‐3β/β‐catenin) activation. Immune infiltration analysis revealed that low SOX9‐AS1 expression was accompanied by a high infiltration of naïve B cells, CD8+ T cells and γδ T cells. In conclusion, SOX9‐AS1 resists TNBC senescence via regulating the Wnt signalling pathway and inhibits immune infiltration. Targeted inhibition of SOX9‐AS1 enhances SASP and thus mobilises immune infiltration to adjunct TIS strategy.
Genome sequences of toxigenic cyanobacteria from a bloom in Lake Mattamuskeet, North Carolina (United States)
Authors: Moretto Jéssica A. , Berthold David E. , Lefler Forrest W. , Mazzei Viviana , Loftin Keith A. , Laughinghouse H. Dail 
Published: November 2024

AbstractLake Mattamuskeet, the largest lake in North Carolina, USA, has undergone decades‐long eutrophication causing reduced water quality and promoting cyanobacterial blooms that may produce toxins. It is therefore necessary to evaluate the cyanobacterial diversity of the lake and their toxigenic potential. We present draft genomes of Microcystis, Pelatocladus, Raphidiopsis, and Umezakia strains isolated from Lake Mattamuskeet. The whole‐genome shotgun projects for Umezakia ovalisporum BLCC‐F208, Microcystis sp. BLCC‐F209, Microcystis sp. BLCC‐F210, Pelatocladus sp. BLCC‐F211, U. ovalisporum BLCC‐F215, and Raphidiopsis BLCC‐F218 have been deposited in GenBank under accession numbers JBHFLK000000000, JBHFLL000000000, CP169647, JBHFLM000000000, JBHFLN000000000, and JBHFLO000000000, respectively. Based on the genomic analysis, several biosynthetic gene clusters (BCGs) with varying degrees of similarity to known toxic and bioactive compound gene clusters were identified across the different cyanobacterial strains.
Estimating the minimal cost of delivering nutrition‐specific and nutrition‐sensitive interventions in Ethiopia
Authors: Maru Yetayesh , Mesfin Firehiwot , Laillou Arnaud , Noor Ramadhani , Adhikari Shiva Raj , Zelalem Meseret , Darsene Hiwot , Memire Solomon , Abie Andarge , Chitekwe Stanley 
Published: November 2024

AbstractThe Ethiopia Food and Nutrition Strategy (FNS 2021–2030) aims to provide evidence‐based, nutrition‐specific, and sensitive interventions to address malnutrition. A costing exercise was done to estimate the minimum financing needed to implement nutrition interventions for the ten‐year FNS, and further analysis was made to estimate the investment required to implement the prioritised recommended Lancet series interventions for 10 years. Activity‐based costing methodology was used to carry out the FNS costing for nutrition interventions prioritised by the different line ministries, and then estimated costs to implement the 2021 recommended Lancets interventions were examined from the FNS. The minimum cost of implementing the National FNS was estimated to be US$ 2.55bn with an average annual cost of US$ 250 million over 10 years (2021–2030). The cost of nutrition‐sensitive approaches represents US$ 1.8 billion (72%) and nutrition‐specific US$ 704 million (28%) of the total cost of the FNS. The Lancet series intervention costs accounted for US$ 1.7 billion (66%) of the total cost of the strategy. In this costing, half of the strong/moderate evidence (7 out of 13) Lancet interventions are costed. Therefore, the strategy's costing should be revised every 3 years to integrate new evidence and consider lessons from real expenditure. Furthermore, the need to establish a nutrition expenditure tracking system is urgent.
Identification of cell‐type specificity, trans‐ and cis‐acting functions of plant lincRNAs from single‐cell transcriptomes
Authors: Xu Jiwei , Shen Enhui , Guo Fu , Wang Kaiqiang , Hu Yurong , Shen Leti , Chen Hongyu , Li Xiaohan , Zhu Qian‐Hao , Fan Longjiang , Chu Qinjie 
Published: November 2024

Summary
Long noncoding RNAs, including intergenic lncRNAs (lincRNAs), play a key role in various biological processes throughout the plant life cycle, and the advent of single‐cell RNA sequencing (scRNA‐seq) technology has opened up a valuable avenue for scrutinizing the intricate roles of lincRNAs in cellular processes.
Here, we identified a new batch of lincRNAs using scRNA‐seq data from diverse tissues of plants (rice, Arabidopsis, tomato, and maize). Based on well‐annotated single‐cell transcriptome atlases, plant lincRNAs were found to possess the same level of cell‐type specificity as mRNAs and to be involved in the differentiation of certain cell types based on pseudo‐time analysis.
Many lincRNAs were predicted to play a hub role in the cell‐type‐specific co‐expression networks of lincRNAs and mRNAs, suggesting their trans‐acting abilities. Besides, plant lincRNAs were revealed to have potential cis‐acting properties based on their genomic distances and expression correlations with the neighboring mRNAs.
Furthermore, an online platform, PscLncRNA (http://ibi.zju.edu.cn/psclncrna/), was constructed for searching and visualizing all identified plant lincRNAs with annotated potential functions. Our work provides new insights into plant lincRNAs at single‐cell resolution and an important resource for understanding and further investigation of plant lincRNAs.
Morphologic transformation of ferrofluid during micropump driving under field control
Authors: Li Wangxu , Li Zhenggui , Han Wei , Wang Ye , Zhao Jiali , Zhou Juping 
Published: November 2024

AbstractThe superiority of ferrofluid pumps in the fields of biomedical, life science, energy, and power research has been experimentally demonstrated. However, the mechanisms underlying the morphological transformations of ferrofluid fusion and separation during pump driving are not completely understood. To bridge the gap between the theory and practical applications of ferrofluid pumps, we employed optical methods to record the dynamic morphological transformation of rotating and fixed ferrofluids under different magnetic field polarities, magnetic field distributions, and ferrofluid mass fractions. The magnetic field polarity causes dynamic differences in the fusion‐separation process of the ferrofluid but does not affect the volume segmentation of the ferrofluid, which depends on the ratio of the magnetic field intensities. When this ratio deviates from one, the morphology of ferrofluid changes, reducing the pumping efficiency. Compared to external environmental factors, the mass fraction does not change the morphology of the ferrofluid. However, high mass fractions lead to wall‐clinging of the ferrofluid, and low mass fractions induce bubbles, both of which detrimentally affect the pumping performance. This study reveals the properties of ferrofluid and the effects of external environmental conditions on the morphological transformation of ferrofluid, providing references for optimizing ferrofluid pumps.
Comparison of the performance of classical and quantum machine‐learning methods on the detection of sugar beet Cercospora leaf disease
Authors: Katirci Ramazan , Adem Kemal , Tatar Muhammed , Ölmez Fatih 
Published: November 2024

AbstractImage processing and machine‐learning (ML) techniques are essential for the detection of diseases and pests in plants. This study explored the application of quantum ML (QML) algorithms for the early detection of Cercospora beticola leaf disease in sugar beet, which causes significant impact on global sugar production. Using a dataset of 1065 images (739 diseased and 326 healthy), we extracted 70 ML statistical features, including 10 from the grey‐level co‐occurrence matrix (GLCM) and 60 colour‐related features. Performance evaluations of classical ML algorithms, such as random forest (RF; 91.95% accuracy) and extreme gradient boosting (91.95% accuracy), demonstrated strong results compared to quantum approaches. Notably, the quantum support vector classifier (QSVC) achieved an accuracy of 85% with perfect recall of 1.00, while the variational quantum classifier (VQC) recorded an accuracy of 88.73%. Dimensionality reduction via principal component analysis reduced features from 70 to 5, enabling effective classification with competitive results: ML (RF) 91.41%, VQC with limited‐memory Broyden–Fletcher–Goldfarb–Shanno with box constraints (L_BFGS_B) 88.73% and QSVC 85%. These findings highlight the potential of QML algorithms in improving agricultural disease identification and aiding in the advancement of more efficient, sustainable farming techniques.
Numerical model of N-level cascade systems for atomic Radio Frequency sensing applications
Authors: Bussey Liam W. , Kale Yogeshwar B. , Winter Samuel , Burton Fraser A. , Lien Yu-Hung , Bongs Kai , Constantinou Costas 
Published: November 2024

AbstractA ready-to-use numerical model has been developed for the atomic ladder (cascade) systems which are widely exploited in Rydberg Radio Frequency (RF) sensors. The model has been explicitly designed for user convenience and to be extensible to arbitrary N-level non-thermal systems. The versatility and adaptability of the model is validated up to 4-level atomic systems by direct comparison with experimental results from the prior art. The numerical model provides a good approximation to the experimental results and provides experimentalists with a convenient ready-to-use model to optimise the operation of an N-level Rydberg RF sensor. Current sensors exploit the 4-level atomic systems based on alkali metal atoms which require visible frequency lasers and these can be expensive and also suffer from high attenuation within optical fiber. The ability to quickly and simply explore more complex N-level systems offers the potential to use cheaper and lower-loss near-infrared lasers.