bluuebunny/crossref_metadata_2025_split huggingface dataset:
Understanding Pathogenic Detection Rates of CNVs in Prenatal Diagnosis: Insights from a Cohort Study
Authors: Zhu Shaohua , Cheng Shibing , Jia Chunyang , liu Furong , Hao Shengju , Lin Pengwu , Zhang Qinghua , Feng Xuan 
Published: October 2024

Abstract

The current study aimed to explore the clinical utility of CNV-seq in prenatal diagnosis by comparing the pathogenic detection rates of copy number variants (CNVs) in pregnant women who underwent amniocentesis for various indications and across different CNV fragment sizes. A total of 1,118 pregnant women who received CNV-seq testing were included in the cohort and categorized into eight groups (Groups A to H) based on their testing indications, with the mixed group (Group H) serving as the reference. The top three groups with the highest pathogenic detection rates were high-risk NIPT (Group A), parental chromosomal abnormalities (Group D), and high-risk trisomy 18 (Group F), with detection rates of 92.16% (141/153), 80% (4/5), and 70% (14/20), respectively. The pathogenic CNV detection rate in our reference group (Group H) was 69.02% (127/184). A binary logistic regression analysis comparing the other seven groups against Group H showed that the detection rate in Group A was significantly higher than that in Group H (P < 0.05), while Groups B, C, E, and G had significantly lower detection rates. Furthermore, based on CNV fragment size, cutoffs were established at 1 Mb and 5 Mb, and the study cohort was further divided into four groups (Groups I to IV). Using the group with multiple CNVs (Group IV) as the reference, the pathogenic detection rates for each group were calculated. Binary logistic regression analysis revealed that Group I had a significantly lower detection rate than Group IV (P < 0.05), while Group III exhibited a significantly higher detection rate (P < 0.05). These findings suggest that abnormal NIPT results are often associated with a higher pathogenic detection rate, and larger CNV fragments exceeding 5 Mb are more likely to be pathogenic. This study provides crucial insights into the clinical application of CNV-seq in prenatal diagnosis, emphasizing the importance of abnormal NIPT results and CNV fragment size in clinical interpretation, thereby facilitating more accurate prenatal risk assessment.
Combined Strategy Of α9-Integrin Transduction and AEIDGIEL Peptide-Functionalized Fibrin Gel Biomaterials to Promote Mature DRG Neurite Growth
Authors: Cimpean Anda , Roll Lars , Reinhard Jacqueline , Kwok Jessica , Faissner Andreas , Fawcett James W. , Jendelová Pavla 
Published: October 2024

Abstract

Spinal cord injury involves complex pathobiological mechanisms, necessitating a multidimensional approach for its cure. Previous studies have shown that α9-integrin expression and activation in mature dorsal root ganglion neurons enable the regeneration of injured axons within the spinal cord. However, tissue cavitation and fibrosis impede the regenerating axons from following their usual pathways, forcing them to seek alternative routes rich in tenascin-C, the primary ligand of the integrin. Fibrin gel can offer three-dimensional support for axonal extension through the cavitated area, preventing the formation of aberrant paths and connections that occur in the absence of a suitable scaffold. The aim of this study was to investigate how combining α9-integrin expression with the use of a fibrin gel as an extracellular microenvironment affects mature DRG neurites growth in vitro. Additionally, we sought to functionalize fibrin with AEIDGIEL peptide, the active domain of tenascin-C, to ensure α9-integrin activation. Our results indicate that fibrin gels are a suitable biomaterial for promoting neurite growth and that AEIDGIEL peptide effectively activates the integrin. In conclusion, the proposed combination therapy of α9-integrin and fibrin gel biomaterials incorporating AEIDGIEL peptide shows promise for addressing the complex challenges of spinal cord injury and promoting effective neural regeneration, laying the foundation for further in vivo research.
Direct synthesis of Cu/ZnO/SiO2 catalyst using induction flow levitation and catalytic activity in methanol production from CO2
Authors: Markov Artyom N. , Golovacheva Anna A. , Kapinos Alexander A. , Dokin Egor S. , Grachev Pavel P. , Petukhov Anton N. , Atlaskin Artem , Vorotyntsev Ilya V. , Suvorov Sergey S. , Vorotyntsev Andrey V. 
Published: October 2024

Abstract

The feasibility of utilizing induction flow levitation technique for synthesizing catalysts aimed at facilitating methanol production was explored in this study. Two distinct types of catalysts were prepared on a silicon dioxide substrate through mechanochemical synthesis utilizing copper and zinc oxide nanopowders: Cu/SiO2 and Cu/ZnO/SiO2. Subsequently, comprehensive characterization of the resulting nanoparticles and catalytic systems was conducted employing a suite of physicochemical techniques. Additionally, the purity assessment of the resultant nanoparticles was accomplished utilizing inductively coupled plasma mass spectrometry. The investigation of catalytic activity was carried out within a continuous-flow reactor, wherein methanol emerged as the predominant reaction product. Over the course of the experiment, a reduction in operating pressure was achieved while upholding elevated methanol productivity and ensuring a notable carbon dioxide conversion rate. Experimental analyses conducted on the pilot plant demonstrated that the catalyst incorporating zinc oxide showcased superior efficiency, attributed to its heightened CO2 and H2 sorption capabilities, consistent with the results of thermoprogrammed desorption. As by-products of the reaction, carbon monoxide, ethanol, methyl formate, dimethyl ether, propanol, isobutyl, and isopropanol were detected.
Allocentric and Egocentric Spatial Attention Bias in Individuals with Right-Hemispheric Stroke and Unilateral Spatial Neglect: A Pilot Study
Authors: Chen Shanjia , Lei Lei , Yu Guo , Yu Jiahui , Huang Jili , Huang Jiyi , he Xiaokuo , Lin Xin 
Published: October 2024

Abstract

Background
In neurorehabilitation, limited research exists on response performance and attention deficits in individuals with neglect across allocentric (aSC) and egocentric (eSC) spatial coding frameworks. These deficits are commonly assessed using pen-and-paper tests, which often fail to detect subclinical lateralized attention deficits. A computer-based task involving detecting shapes using a cue-to-target paradigm offers precise and sensitive data for identifying allocentric and egocentric spatial attention deficits.
Methods
This pilot study involved 18 right-handed healthy volunteers (HC) aged 23.89 ± 3.44 years, 13 individuals with right-hemispheric stroke and neglect (USN+) aged 32.83 ± 4.45 years, and 10 with stroke but no neglect (USN-) aged 32.6 ± 5.18 years, who performed a computer-based attention task. Response time (RT), accuracy rate (AR), lateralized visual perception deficits, reorienting spatial attention deficits, and disengagement deficits were recorded and analyzed.
Results
The USN + group had lower AR and slower RT in both aSC and eSC conditions than the USN- and HC groups across visual fields and cue types. Notably, the USN + group showed a greater RT delay for left and right visual fields (LVF) targets in the contralesional visual field than in the ipsilesional field, a pattern absent in the USN- or HC. No significant differences in AR and total RT were found between the aSC and eSC groups, but both stroke groups took longer to identify LVF targets in aSC than in eSC. Only the HC group displayed a significantly positive validity effect, with slower RT after invalid cues. This reflected their ability to disengage attention and maintain alertness. The USN + group had significantly slower RT for invalidly cued contralesional targets compared to the USN- and HC groups both in conditions.
Conclusion
Our study shows that individuals with neglect exhibit a rightward attentional bias during visuospatial tasks in both aSC and eSC conditions. Individuals without neglect, though classified as subclinical by pen-and-paper tests, showed a rightward bias in aSC and a leftward bias in eSC tasks using computer-based assessments. Those with or without neglect had greater difficulty with allocentric target identification in the contralesional visual field compared to egocentric processing. Additionally, individuals with neglect demonstrated a disengagement deficit, while both groups showed reorienting deficits in visuospatial attention.
Bias-Switchable Photomultiplication and Photovoltaic Dual-Mode Flexible Near-Infrared Organic Photodetector
Authors: Wang Yazhong , Huang Yijun , Shao Lin , Hao Lu , Zheng Jie , Cao Yunhao , Li Shuaiqi , Tan Zhaohong , Li Shuai , Zhong Wenkai , Dong Sheng , Yang Xiye , Benduhn Johannes , Liu Chunchen , Leo Karl , Huang Fei 
Published: October 2024

Abstract

Photomultiplication-type organic photodetectors (PM-OPDs) offer signal amplification, ideal for faint light detection and simplified systems, but typically face slow response speeds and high dark currents. In contrast, photovoltaic-type OPDs (PV-OPDs) provide fast response and high detectivity (D*) but have limited photoresponse. This study introduces a non-fullerene acceptor, BFDO-4F, into the active layer to create trap states for photogenerated electron capture. The resulting device exhibits dual-mode characteristics and is bias-switchable between PV and PM-modes. It achieves a high D* of 1.92×10¹² Jones and fast response times of 2.83/4.43 µs in PV-mode, and exceptional EQEs up to 3484% in PM-mode. Notably, an on-chip self-powered module featuring PV-mode pixels driving a PM-mode pixel is demonstrated, yielding a photocurrent approximately five times higher than the reference device. This approach establishes a new framework for developing multifunctional, bias-switchable, dual-mode on-chip OPDs, unlocking diverse opportunities in highly integrated advanced sensing applications.
Extraction of banana fiber and characterization analysis of a hybrid (banana, jute, carbon and glass) reinforced composite material.
Authors: Based Md. Abdul 
Published: October 2024

Abstract

Banana is a well-known tree in many countries. After harvesting bananas, the trees are cut down. The fibers obtained through this plant processing acquire luxurious properties like high strength, toughness, durability, etc. Due to the micro-pores on its surface, it has good reinforcing ability with resins. In many engineering applications today, including the automotive industry, natural fiber composites or composites that combine natural fibers with high performance fibers are preferred over synthetic fiber composites. Here a simple process is adopted to extract the banana fiber where no machines or chemicals are used. Three types of composite materials are made from banana, carbon, jute, and glass fiber, as well as their yarns, fabrics and mats. Three different samples, namely S1, S2, and S3, were prepared using the hand lay-up technique. After analyzing their properties, it showed that the highest mechanical properties are found in the composite of [Carbon fabric-(jute+banana) blended yarn-glass mat] at the direction of Yarn. The tensile strengths of samples S1, S2, and S3 are 229 MPa, 289 MPa, and 223 MPa, respectively, with elongation values of 6.47%, 5.59%, and 7.24%. For the flexural strength test, the results are 247 MPa for S1, 294 MPa for S2, and 234 2 MPa for S3. Finally, the impact strength results show 22 J for S1, 15 J for S2, and 19 J for S3. The qualities of the above features ensure that they can be used in the automobile industry.
A New Integral Transform and Applications: HK-Transform
Authors: Hilmi Hozan , HamaFaraj Jwamer Karwan 
Published: October 2024

In this paper, we propose a new integral transform called the Transform and employ it to analytically solve the Volterra integral equations (VIE.) of the first kind. To achieve this First we derive the transform of basic algebraic and transcendental mathematical functions. The fundamental characteristics of the transform, which can be used to solve a variety of functional equations, including, we then discuss the fundamental properties, such as ordinary differential equations and integral equations, then we discover the exact solution for general first class V.I.E. To illustrate the applicability of the transform, numerical problems are examined and handled carefully step-by-step. The results show that the proposed Transform new integral transform produces accurate solutions for first-kind VIE. without requiring time-consuming computations.
Периоперационное кондиционирование оксидом азота для предотвращения острого почечного повреждения при кардиохирургических вмешательствах у пациентов с хронической болезнью почек: промежуточные результаты рандомизированного контролируемого исследования DEF
Authors: Каменщиков Николай Олегович , Тё М. А. , Подоксенов Ю. К. , Кравченко И. В. , Чурилина Е. А. , Козулин М. С. , Свирко Ю. С. , Гусакова А. М. , Козлов Б. Н. 
Published: October 2024

INTRODUCTION: Acute kidney injury (AKI) is a common and serious complication after cardiac surgery performed under cardiopulmonary bypass (CPB). Patients with chronic kidney disease (CKD) are particularly vulnerable to cardiac surgery-associated AKI. Nitric oxide (NO) has great potential for implementing multi-organ protection in cardiac surgery.
OBJECTIVES: To test the hypothesis that perioperative nitric oxide conditioning has a good safety profile and reduces the incidence of acute kidney injury during cardiac surgery under cardiopulmonary bypass in patients with chronic kidney disease. MATERIAL AND METHODS: An interim analysis of the results of the single-center prospective randomized controlled trial DEFENDER was conducted. Currently, the study included 96 patients with CKD who underwent cardiac surgery under CPB. Patients was randomized into 2 equal groups consisting of 48 people each. In the experimental group, during the operation and 6 hours after it, 80 ppm NO was delivered through the ventilator and CPB circuits. In the control group, during the operation and 6 hours after it, a standard oxygen-air mixture without NO was delivered through the ventilator and CPB circuits. The end points of the study were: the difference between groups in the incidence of AKI, the incidence of postoperative complications, outcomes and the safety profile of NO therapy. RESULTS: In the experimental group, the incidence of AKI was significantly lower compared to the control group (16,7 и 35,4 %, p = 0.036). In the experimental group, nitrogen dioxide concentration in the gas-air mixture and methemoglobin level were not above the reference range. The groups did not differ in the frequency of blood transfusions, the amount of postoperative bleeding, or the number of platelets on the first day after surgery. CONCLUSIONS: Perioperative nitric oxide conditioning has a good safety profile and reduces the incidence of acute kidney injury during cardiac surgery with CPB in patients with chronic kidney disease.
Эффективность алгоритма назначения диуретической терапии под контролем натрийуреза у пациентов с острой декомпенсацией сердечной недостаточности в отделении реанимации и интенсивной терапии: проспективное интервенционное контролируемое исследование
Authors: Кобалава Жанна Давидовна , Толкачева В. В. , Назаров И. С. , Галочкин С. А. , Вацик-Городецкая М. В. , Петриков С. С. 
Published: October 2024

INTRODUCTION: The European Society of Cardiology suggests using early and repeated assessment of sodium content in urine in patients admitted with heart failure to evaluate diuretic therapy. OBJECTIVES: Evaluation of the effectiveness of the algorithm for diuretic therapy under the control of natriuresis in patients with acute decompensation of chronic heart failure (ADCHF) in the intensive care unit (ICU). MATERIALS AND METHODS: A prospective study included 150 patients hospitalized in the ICU with ADCHF. All patients underwent standard physical, laboratory and instrumental examinations. The starting dose of furosemide was 20 mg i/v. After 2 hours, a quantitative assessment of sodium in urine was performed. If the natriuresis was ≥ 70 mmol/l, furosemide was continued at the same dose every 12 hours. If the natriuresis was < 70 mmol/L, the dose of furosemide was doubled. Natriuresis was monitored every 12 hours. RESULTS: In the natriuresis-controlled group the duration of patients' stay in the ICU was significantly shorter (3 vs. 5 days, p = 0.01), achieving euvolemia was significantly more frequent (42 % vs. 12 %, p < 0.05), the daily diuresis in patients was significantly higher on more daily (120 mg vs. 80 mg, p < 0.05) and the total dose of furosemide (420 mg vs. 240 mg, p < 0.05). There was a pronounced decrease in congestion in the group of patients with natriuresis control with less pulmonary (number of B-lines 9.6 ± 1.2 versus 23.4 ± 2.5) and venous congestion (GRADE 0–56 %, 1–20 %, 2–24 %, 3–0 % against 0–20 %, 1–35 %, 2–18 %, 3–27 %) on the 3rd day of hospitalization. CONCLUSION: The inclusion of an algorithm for diuretic therapy under the control of natriuresis in patients with ADCHF in the ICU is effective and contributes to a more pronounced and rapid reduction of congestion, as well as a 1.5-fold reduction of time in the ICU.
Предикторы госпитальной летальности при экстренном хирургическом лечении острейшего расслоения аорты типа А по Стенфорду в условиях искусственного кровообращения: ретроспективное когортное исследование
Authors: Журавель Сергей Владимирович , Иванов И. В. , Владимиров В. В. , Сагиров М. А. , Редкобородый А. В. , Сергеева И. С. , Коков Л. С. 
Published: October 2024

INTRODUCTION: Acute Stanford type A aortic dissection is one of the most severe cardiovascular diseases with high in-hospital mortality. OBJECTIVE: To establish predictors of in-hospital mortality in the treatment of patients with acute Stanford type A aortic dissection under conditions of artificial circulation and to determine their threshold values. MATERIALS AND METHODS: Study design: retrospective, cohort, single-center. Inclusion criteria: surgical intervention with cardiopulmonary bypass, confirmed diagnosis of acute Stanford type A aortic dissection, age > 18 years. The study included 51 patients: 42 men and 9 women aged from 35 to 77 years, median 59 years [47; 65]. All patients (n = 51) were treated in 2022 and were divided into 2 groups depending on the outcome: group 1 (n = 27) included those who died in the intensive care unit within 30 days, group 2 (n = 24) included survived and discharged patients. The endpoint of trial was 30-day in-hospital mortality. RESULTS: Postoperative Sequential Organ Failure Assessment (SOFA) score > 7, Acute Physiology And Chronic Health Evaluation (APACHE) II score > 19, and Vasoactive-inotropic score (VIS) > 10, cardiopulmonary bypass time > 177 minutes, and total hypothermic circulatory arrest time > 30 minutes were associated with an increased risk of 30-day in-hospital mortality. CONCLUSIONS: Statistically significant predictors of hospital mortality in the treatment of patients with acute Stanford type A aortic dissection under cardiopulmonary bypass are the SOFA index, the APACHE II index and the VIS index after the end of surgery, as well as the time of cardiopulmonary bypass and the time of total hypothermic circulatory arrest.
Сравнение методик пункционно-дилатационной трахеостомии Фрова и Григгс у пациентов с избыточной массой тела и ожирением: рандомизированное контролируемое исследование
Authors: Кругляков Николай Михайлович , Альтшулер Н. Э. , Карпова О. В. , Кукленко А. Е. , Киселёв К. В. , Попугаев К. А. 
Published: October 2024

INTRODUCTION: Tracheostomy is one of the most frequently performed operations in intensive care patients. This is due to the fact that in the population of intensive care patients, puncture tracheostomy has a number of advantages compared to surgical tracheostomy (ST). This determines the urgency of finding ways to reduce the risk of perioperative complications during puncture tracheostomy in intensive care patients, especially in overweight and obese patients. OBJECTIVE: To study the advantages and limitations of puncture dilation tracheostomy (PDT) performed using the Frova method under ultrasound control in intensive care patients with overweight and obesity, in comparison with the Griggs PDT method under fibrobronchoscopic (FBS) control. MATERIALS AND METHODS: The presented prospective study was conducted in the departments of anesthesiology and intensive care of the A.I. Burnasyan Federal Medical Biophysical Center FMBA from 2017 to 2021, which included 92 overweight and obese patients whose body mass index (BMI) was more than 25 kg/m2 and required tracheostomy. Two groups were identified: group (n = 48) — Frova tracheostomy under ultrasound control, group (n = 44) — Griggs tracheostomy under FBS control. RESUITS: Performing PDT using the Frova technique under ultrasound control is feasible and safe in intensive care patients with overweight and obesity, whereas with grade 3 obesity with a BMI of more than 41 kg/m2 and/or with a thickness of pretracheal soft tissues of more than 40 mm, PDT using the Frova technique should be abandoned and should be considered as absolute contraindications for performing PDT using the Frova method under ultrasound control. CONCLUSIONS: PDT using the Frova method under ultrasound control, when compared with the Griggs method with FBS control, can significantly reduce the incidence of intraoperative desaturation, arterial hypotension, intraoperative severe hemorrhagic complications, postoperative soft tissue infection around a tracheostomy wound.
Factors associated with recurrent stroke in Sikhio District, Nakhon Ratchasima Province, Thailand
Authors: Singsalasang Atthawit , Bandidphak Jitra 
Published: October 2024

Background: Stroke survivors can experience residual disability and are at risk for subsequent strokes that might cause further damage or even death. The objective of this study was to identify factors associated with recurrent stroke in Sikhio District, Nakhon Ratchasima Province, Thailand.
Method: We used gender and age characteristics to match our study participants in a ratio of 1 case patient to 2 controls. The total sample size was 111 participants, 37 participants with recurrent stroke were considered as the case group and 74 participants without recurrent stroke were the control group. The case group was diagnosed by a physician along with a computerized tomography (CT) scan and received treatment at Sikhio Hospital in the period of 1 October 2021 - 30 September 2022. Face-to-face data were collected by a structured questionnaire and compared to the medical record.
Results: The study revealed that risk factors associated with recurrent stroke in Sikhio District were comorbidities (AOR = 4.64, 95% CI = 1.35-15.86, p < 0.014) and systolic blood pressure (AOR = 2.41, 95% CI = 1.10-5.78, p < 0.049).
Conclusion: Comorbidities and systolic blood pressure represented a risk to recurrent stroke among post-stroke patients. Therefore, hospitals should find effective methods to care for patients with co-morbidities and promote knowledge about blood pressure control.