bluuebunny/crossref_metadata_2025_split huggingface dataset:
The methylation of Notch1 promoter mediates the osteogenesis differentiation in human aortic valve interstitial cells through Wnt/β‐catenin signaling
Authors: Zhou Yangzhao , Li Jianming , Zhou Kang , Liao Xiaobo , Zhou Xinmin , Shen Kangjun 
Published: April 2019

AbstractAortic valve interstitial cells (AVICs) have the potential to undergo calcification, which has been regarded as a critical issue during the pathology of calcific aortic valve disease (CAVD). In the past decade, epigenetics, in particular, DNA methylation dysregulation, has been reported to play a vital role in the occurrence and development of CAVD. In the present study, the expression of Notch1, which can inhibit the osteogenesis differentiation of valve interstitial cells, was downregulated whereas the expression of methyltransferases was upregulated in CAVD tissues, suggesting the potential role of DNA methylation in Notch1 expression and CAVD progression. As revealed by DNA extraction and bisulfite sequencing polymerase chain reaction (PCR), the methylation level in Notch1 promoter was much higher in CAVD tissues and human AVICs on Day 14 of osteogenesis differentiation induction. The silence of Notch1 intercellular domain (NICD) promoted while the treatment of demethylation agent, 5‐Aza‐dC, inhibited the osteogenesis differentiation. Moreover, NICD overexpression significantly suppressed the transcriptional activity of β‐catenin on TCF4, and the expression of osteogenesis differentiation factors, indicating the involvement of Wnt/β‐catenin signaling in Notch1 modulating the osteogenesis differentiation in human AVICs (hAVICs). Taken together, Notch1 promoter methylation leads to a decreased Notch1 expression and subsequent decreased release of NICD in the nucleus of hAVICs, therefore promoting the activation of Wnt/β‐catenin signaling and the expression of osteogenesis differentiation factors, finally promoting the osteogenesis differentiation in hAVICs. DNA methylation might act as an important bridge to link epigenetic variation and CAVD progression.
Epigenetic‐based therapy for colorectal cancer: Prospect and involved mechanisms
Authors: Rezapour Saleheh , Hosseinzadeh Elham , Marofi Faroogh , Hassanzadeh Ali 
Published: April 2019

AbstractEpigenetic modifications are heritable variations in gene expression not encoded by the DNA sequence. According to reports, a large number of studies have been performed to characterize epigenetic modification during normal development and also in cancer. Epigenetics can be regarded more widely to contain all of the changes in expression of genes that make by adjusted interactions between the regulatory portions of DNA or messenger RNAs that lead to indirect variation in the DNA sequence. In the last decade, epigenetic modification importance in colorectal cancer (CRC) pathogenesis was demonstrated powerfully. Although developments in CRC therapy have been made in the last years, much work is required as it remains the second leading cause of cancer death. Nowadays, epigenetic programs and genetic change have pivotal roles in the CRC incidence as well as progression. While our knowledge about epigenetic mechanism in CRC is not comprehensive, selective histone modifications and resultant chromatin conformation together with DNA methylation most likely regulate CRC pathogenesis that involved genes expression. Undoubtedly, the advanced understanding of epigenetic‐based gene expression regulation in the CRC is essential to make epigenetic drugs for CRC therapy. The major aim of this review is to deliver a summary of valuable results that represent evidence of principle for epigenetic‐based therapeutic approaches employment in CRC with a focus on the advantages of epigenetic‐based therapy in the inhibition of the CRC metastasis and proliferation.
The role of autophagy in maintaining intestinal mucosal barrier
Authors: Wu Yanping , Tang Li , Wang Baikui , Sun Qiming , Zhao Pengwei , Li Weifen 
Published: April 2019

AbstractThe intestinal mucosal barrier is the first line to defense against luminal content penetration and performs numerous biological functions. The intestinal epithelium contains a huge surface that is lined by a monolayer of intestinal epithelial cells (IECs). IECs are dominant mediators in maintaining intestinal homeostasis that drive diverse functions including nutrient absorption, physical segregation, secretion of antibacterial peptides, and modulation of immune responses. Autophagy is a cellular self‐protection mechanism in response to various stresses, and accumulating studies have revealed its importance in participating physiological processes of IECs. The regulatory effects of autophagy depend on the specific IEC types. This review aims to elucidate the myriad roles of autophagy in regulating the functions of different IECs (stem cells, enterocytes, goblet cells, and Paneth cells), and present the progress of autophagy‐targeting therapy in intestinal diseases. Understanding the involved mechanisms can provide new preventive and therapeutic strategies for gastrointestinal dysfunction and diseases.
IRF2BP2: A new player in the regulation of cell homeostasis
Authors: Ramalho-Oliveira Renata , Oliveira-Vieira Barbara , Viola João P B 
Published: April 2019

Abstract
The IRF2BP2 (IFN regulatory factor 2 binding protein 2) protein was identified as a nuclear protein that interacts with IFN regulatory factor 2 (IRF-2) and is an IRF-2-dependent transcriptional repressor. IRF2BP2 belongs to the IRF2BP family, which includes IRF2BP1, IRF2BP2, and IRF2BPL (EAP1). Recently, IRF2BP2 has emerged as an important new transcriptional cofactor in different biological systems, acting as a positive and negative regulator of gene expression. IRF2BP2 plays a role in different cellular functions, including apoptosis, survival, and cell differentiation. Additionally, IRF2BP2 may be involved in cancer development. Finally, it has been recently reported that IRF2BP2 may play a role in macrophage regulation and lymphocyte activation, highlighting its function in innate and adaptive immune responses. However, it has become increasingly clear that IRF2BP2 and its isoforms can have specific functions. In this review, we address the possible reasons for these distinct roles of IRF2BP2 and the partner proteins that interact with it. We also discuss the genes regulated by IRF2BP2 during the immune response and in other biological systems.
Useful manifestations to detect adenovirus in children with upper respiratory infections: A retrospective study
Authors: Takahashi Kenichiro , Fujimoto Tsuguto , Hanaoka Nozomu , Tamura Mariko , Suzuki Yoko , Sugihara Shigetaka 
Published: April 2019

AbstractObjectiveApproximately 1 million adenovirus immunochromatography (IC) kits are annually used in Japan. However, no practical strategies have been developed regarding their use for detecting adenovirus. The present study aims to verify the usefulness of clinical manifestations in making decisions regarding the use of adenovirus IC kits for children with upper respiratory infections (URI).MethodsThe medical records of 825 pediatric cases tested by IC kits for adenovirus were extracted from clinical laboratory department database over a 3‐year period at our hospital. Among them, 585 patients were suspected adenovirus URI, and their clinical manifestations were reviewed. After data cleaning, 10 types of clinical manifestations were statistically analyzed between adenovirus IC kit‐positive and ‐negative groups. Multivariate analysis was performed to select significant clinical manifestations using adenovirus IC kit positivity as the objective variable.ResultsAmong 585 pediatric patients, the cases of 420 patients, with suitable data for whom no other pathogen was detected, were reviewed. Adenovirus was detected in 86 cases. Multivariate analysis identified a significant difference for three clinical manifestations: (1) fever ≥ 39.0°C, (2) rhinorrhea, and (3) tonsillar exudate. The negativity rate for the IC kit was 90% when none of the three manifestations was observed.ConclusionsThe results suggested that IC kits for adenovirus tend to give negative results in cases that lack all the three above mentioned clinical manifestations.
A disintegrin‐like and metalloproteinase with thrombospondin‐1 (ADAMTS‐1) levels in gingival crevicular fluid correlate with vascular endothelial growth factor‐A, hypoxia‐inducible factor‐1α, and clinical parameters in patients with advanced periodontitis
Authors: Tayman Mahmure Ayşe , Kurgan Şivge , Önder Canan , Güney Zeliha , Serdar Muhittin A. , Kantarcı Alpdoğan , Günhan Meral 
Published: April 2019

AbstractBackgroundADAMTS (a disintegrin‐like and metalloproteinase with thrombospondin) are a family of proteinases that are structurally similar to the family of matrix metalloproteinases with critical roles in damage and repair of the extracellular matrix. Their functions are closely related to inflammation, hypoxia, and vascularization. Our aim was to determine levels of ADAMTS‐1 in gingival crevicular fluid (GCF) in patients with advanced periodontal diseases and identify their association with hypoxia‐inducible factor‐1alpha (HIF‐1α), vascular endothelial growth factor (VEGF‐A), and clinical parameters of periodontitis.MethodsThe study consisted of three groups: healthy individuals (control; n = 20), generalized chronic periodontitis (CP; n = 21), and generalized aggressive periodontitis (GAgP; n = 20). Clinical parameters were measured. Levels of ADAMTS‐1, VEGF‐A, and HIF‐1α in GCF and serum were quantified by enzyme‐linked immunosorbent assay (ELISA) and reported as total amounts and concentration.ResultsADAMTS‐1 total amount in GCF were significantly higher in patients with CP and GAgP compared with healthy individuals (P < 0.05). HIF‐1α total amount in GCF were also higher in periodontitis groups compared with the control group (P < 0.05). GCF total VEGF‐A content was significantly higher in the GAgP group compared with the CP and the controls (respectively; P = 0.023, P = 0.003). There was a significant correlation between ADAMTS‐1, VEGF‐A, and HIF‐1α levels in the GCF and clinical periodontal parameters (probing depth [PD], bleeding on probing [BOP], and clinical attachment loss (CAL); P < 0.05).ConclusionADAMTS‐1 may play a role in advanced periodontal disease pathogenesis in correlation with tissue hypoxia and vascularization.
Identification of a novel salivary biomarker miR‐143‐3p for periodontal diagnosis: A proof of concept study
Authors: Nisha K.J. , Janam Presanthila , Harshakumar K. 
Published: April 2019

AbstractBackgroundThough the use of salivary miRNAs as potential biomarkers has been reported in few diseases/conditions such as rheumatoid arthritis and oral cancer, there are no reported studies on their utility in periodontal diagnostics. Thus, the aim of the present study was to profile salivary miRNAs and identify the most suitable salivary miRNA biomarker in chronic periodontitis.MethodsIn this study, we have explored the potential application of next generation sequencing (NGS) technology for profiling miRNAs in two unstimulated saliva samples collected by passive drool method from a patient diagnosed with generalized chronic periodontitis and a healthy control. Subsequently, the validation of most highly expressed known miRNA in periodontitis was performed in saliva samples collected from an independent set of 16 chronic periodontitis patients and 16 periodontally healthy controls using quantitative real‐time PCR (qRT‐PCR). Target gene prediction and pathway mapping were performed using bioinformatic tools.ResultsNGS analysis identified 40 upregulated and 40 downregulated known miRNAs in chronic periodontitis compared to healthy controls, of which miR‐143‐3p was the most highly expressed miRNA in periodontitis (Read count – 227630; fold change – 5.82). Validation using qRT‐PCR showed significant upregulation of miR‐143‐3p expression in the test group compared with controls (P < 0.05). K‐RAS (V‐Ki‐ras2 Kirsten rat sarcoma viral oncogene) gene was predicted as the target gene for miR‐143‐3p in humans. KEGG (Kyoto Encyclopedia of genes and genomes) pathway mapping revealed the involvement of K‐RAS in mitogen‐activated protein kinases (MAPK) pathway.ConclusionsThe application of NGS for miRNA expression profiling can be considered a valuable tool in detection of novel biomarkers in periodontal diagnostics. Also, the results of the study points to the potential utility of miR143‐3p as a novel salivary biomarker for chronic periodontitis.
The applicability of Porter's generic strategies in pure online firms: A case study approach
Authors: Firoz Suleman Marwa , Rashidirad Mona , Firoz Suleman Saffa 
Published: April 2019

AbstractThe aim of this paper is to investigate the usability and applicability of Porter's generic strategies in the current e‐business environment. In this paper, a review of Porter's generic strategies was conducted to recognise the limitations and weaknesses of the model, which prevent its effective application in the e‐business environment. To do so, a case study approach on three pure online firms, e.g., Amazon, e‐bay and Google was employed. The findings acknowledged the application of Porter's generic strategies to pure online firms in order to achieve competitive advantage and proved the positive impact of generic strategies on firms' performance. The paper suggests that although cost leadership strategy is able to generate greater performance, pursuing a differentiation strategy can be more worthwhile and sustainable for pure online firms compared to cost leadership strategy. This research contributes to existing research by suggesting Porter's generic strategies as a valid and viable model for pure online firms which operate in the current e‐business environment.
Bulk storage of mango (Mangifera indica L.) and pineapple (Ananas comosus L.) pulp: effect of pulping and storage temperature on phytochemicals and antioxidant activity
Authors: Arampath Palitha C , Dekker Matthijs 
Published: April 2019

AbstractBACKGROUNDThe effects of pulp extraction, thermal treatment and bulk storage of mango (Mangifera indica L.) and pineapple (Ananas comosus L.) pulps for 20 weeks at ambient (28 ± 2 °C) and cold (4 °C) temperatures on the bioactive phytochemicals and antioxidant activity were investigated.RESULTSThe contents of total polyphenols in mango (10.5%) and pineapple (5.4%) increased during pulping. The ratio of the degradation rate constants (kd values) (28 ± 2 °C: 4 °C) of vitamin C, polyphenols, Trolox equivalent antioxidant capacity (TEAC) and β‐carotene ranged from 2–4.5 and 1.5–2.7 in mango and pineapple pulps, respectively. The kd values of tannic acid, chlorogenic acid, epicatechin and catechin in mango pulp were 1.5–1.8 times higher under ambient storage than in cold storage. Furthermore, in pineapple pulp, the degradation rates of the same components were 1.6, 1.6, 2.1 and 1.4 times, respectively, faster at room temperature than in cold storage. The bulk storage of pulps at 4 °C provided better retention of health‐promoting compounds than ambient temperature storage for up to 20 weeks.CONCLUSIONBulk storage of mango and pineapple pulp under cold storage conditions (4 °C) is recommended as a better pulp preservation method than storage at ambient (28 ± 2 °C) temperature. © 2019 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
Long‐term outcomes after endoscopic dacryocystorhinostomy without mucosal flap preservation
Authors: Kingdom Todd T. , Barham Henry P. , Durairaj Vikram D. 
Published: April 2019

ObjectiveA growing body of evidence demonstrates that endonasal endoscopic dacryocystorhinostomy (eDCR) techniques provide comparable results to conventional external techniques. The purpose of this study was to evaluate long‐term outcomes after powered endoscopic DCR without preservation of mucosal flaps for the management of acquired nasolacrimal duct obstruction performed by a single surgical team.MethodsA retrospective review was performed of patients with epiphora secondary to acquired nasolacrimal duct obstruction who underwent eDCR without mucosal flap preservation from May 2003 to April 2013 at a tertiary referral medical center. Main outcome measures were subjective improvement in epiphora and assessment of anatomic patency based on lacrimal irrigation and endoscopic evaluation.ResultsEighty patients (69 primary and 11 revision) totaling 103 procedures (87 primary, 16 revision) were available for analysis at a mean follow‐up of 28.7 (range 6–114) months. At the most recent follow‐up, 92 of 103 (89.3%) procedures had complete resolution of epiphora, and 10 of 103 procedures achieved mild intermittent epiphora. Complete resolution of epiphora was noted in 93.1% (81 of 87) of primary procedures and 68.8% (11 of 16) of revision procedures. Objective anatomic patency was confirmed in 98% (101 of 103). A revision procedure was required in 5.8% (6 of 103).ConclusionOutcomes of powered eDCR without the preservation of mucosal flaps compare favorably to previously reported results in the literature. These long‐term results suggest that mucosal flap preservation is not required to achieve successful outcomes with eDCR.Level of Evidence4 Laryngoscope, 130:12–17, 2020
Treatment modalities in sinonasal mucosal melanoma: A national cancer database analysis
Authors: Ganti Ashwin , Raman Anish , Shay Aryan , Kuhar Hannah N. , Auger Samuel R. , Patel Tirth , Kuan Edward C. , Diaz Aidnag Z. , Batra Pete S. , Tajudeen Bobby A. 
Published: April 2019

Objectives/HypothesisThe purpose of this study was to investigate the association of demographic factors, tumor stage, and treatment modalities for overall survival in patients with sinonasal mucosal melanoma (SNMM).Study DesignRetrospective database review.MethodsThe National Cancer Database was queried for patients of all ages with SNMM between 2004 and 2015. Univariate Kaplan‐Meier and multivariate Cox regression analyses were performed to evaluate the association of suspected prognostic factors with overall survival.ResultsA total of 1,874 patients with SNMM were included in the analysis. The 5‐year overall survival was 24%. Prognostic factors associated with decreased survival include advanced age (hazard ratio [HR]: 1.02, 95% confidence interval [CI]: 1.01‐1.03), T4 disease (HR: 1.44, 95% CI: 1.09‐1.89), and presence of distant metastases (HR: 3.22. 95% CI: 2.06‐5.04). Improved survival was associated with surgical resection only when margins were negative (HR: 0.44; 95% CI: 0.30‐0.65). In patients with metastatic disease, administration of immunotherapy (HR: 0.14; 95% CI: 0.04‐0.49) was associated with improved survival. Surgical approach, radiotherapy, and chemotherapy were nonsignificant predictors of survival.ConclusionsThis investigation is the largest to date to analyze the association of treatment modalities with overall survival in SNMM. Surgery remains the mainstay of treatment in patients with SNMM. However, administration of immunotherapy may confer survival benefit to patients with metastatic disease.Level of EvidenceNA Laryngoscope, 130:275–282, 2020
Water or soil—What is the dominant driver controlling the vegetation pattern of degraded shallow mountain peatlands?
Authors: Glina Bartłomiej , Piernik Agnieszka , Hulisz Piotr , Mendyk Łukasz , Tomaszewska Klara , Podlaska Magda , Bogacz Adam , Spychalski Waldemar 
Published: April 2019

AbstractThe aim of this paper was to evaluate the link between environmental conditions and the vegetation pattern of mountain peatlands drained for forestry. We assumed that (a) water chemistry and soil properties differ between the investigated peatlands types—from fen to bog, (b) the vegetation pattern is dependent on water chemistry and soil properties, and (c) water chemistry and soil properties play different roles in determining peatland patterning. For our study, five ecologically and topographically diverse forestry‐drained shallow peatlands in Central Sudetes, Poland, were selected. A comparison between the studied peatlands and environmental variables was done by discriminant analysis, whereas vegetation–environmental relationship was analysed by canonical correspondence analysis. Results demonstrated that pH, HCO3−, NO3−, and Ca2+ best explained the variation in water chemistry (approximately 74%), whereas base saturation, pHwater, and plant‐available phosphorus best explained the variation in soil properties. Plant assemblages within the peatlands exhibited three vegetation clusters that did not always correspond to peatland ecological type. The vegetation was mostly affected by water chemistry (explained up to 54% of variation), rather than by soil properties. Vegetation within such ecosystems seems to be a good indicator of differences in water chemistry, caused by differences in bedrock (soligenic fen peatlands) or atmospheric inputs (ombrogenic bogs). Our results will help improve our understanding of vegetation–environment relationships in degraded mountain peatland ecosystems in the temperate climate zone. They might also be useful for proper planning of restoration and monitoring of these ecosystems.
A Wide‐Bandgap Conjugated Polymer Based on Quinoxalino[6,5‐f  ]quinoxaline for Fullerene and Non‐Fullerene Polymer Solar Cells
Authors: Pang Shuting , Liu Liqian , Sun Xiaofei , Dong Sheng , Wang Zhenfeng , Zhang Ruiwen , Guo Yiting , Li Weiwei , Zheng Nan , Duan Chunhui , Huang Fei , Cao Yong 
Published: April 2019

AbstractA wide‐bandgap conjugated polymer, PNQx‐2F2T, based on a ring‐fused unit of quinoxalino[6,5‐f  ]quinoxaline (NQx), is synthesized for use as electron donor in polymer solar cells (PSCs). The polymer shows intense light absorption in the range from 300 to 740 nm and favorable energy levels of frontier molecular orbitals. The polymer has afforded decent device performance when blended with either fullerene‐based acceptor [6,6]‐phenyl‐C71‐butylric acid methyl ester ([70]PCBM) or non‐fullerene acceptor 3,9‐bis(2‐methylene‐(3‐(1,1‐dicyanomethylene)‐indanone‐methyl))‐5,5,11,11‐tetrakis(4‐n‐hexylphenyl)‐dithieno[2,3‐d: 2′,3′‐d′]‐s‐indaceno[1,2‐b:5,6‐b′]dithiophene (IT‐M). The highest PCEs of 7.9% and 7.5% have been achieved for [70]PCBM or IT‐M based PSCs, respectively. Moreover, the influence of molecular weight of PNQx‐2F2T on solar cell performance has been investigated. It is found that fullerene‐based devices prefer higher polymer molecular weight, while non‐fullerene devices are not susceptible to the molecular weight of PNQx‐2F2T. The device results are extensively explained by electrical and morphological characterizations. This work not only evidences the potential of NQx for constructing high‐performance photovoltaic polymers but also demonstrates a useful structure–performance relationship for efficiency enhancement of non‐fullerene PSCs via the development of new conjugated polymers.