bluuebunny/crossref_metadata_2025_split huggingface dataset:
Wnt signaling inhibition by monensin results in a period of Hippo pathway activation during intestinal adaptation in zebrafish
Authors: Isani Mubina A. , Gee Kristin , Schall Kathy , Schlieve Christopher R. , Fode Alexa , Fowler Kathryn L. , Grikscheit Tracy C. 
Published: March 2019

Intestinal adaptation (IA) is a critical response to increase epithelial surface area after intestinal loss. Short bowel syndrome (SBS) may follow massive intestinal resection in human patients, particularly without adequate IA. We previously validated a model in zebrafish (ZF) that recapitulates key SBS pathophysiological features. Previous RNA sequencing in this model identified upregulation of genes in the Wnt and Hippo pathways. We therefore sought to identify the timeline of increasing cell proliferation and considered the signaling that might underpin the epithelial remodeling of IA in SBS. SBS was created in a ZF model as previously reported and compared with sham fish with and without exposure to monensin, an ionophore known to inhibit canonical Wnt signaling. Rescue of the monensin effects was attempted with a glycogen synthase kinase 3 inhibitor that activates wnt signaling, CHIR-99021. A timeline was constructed to identify peak cellular proliferation, and the Wnt and Hippo pathways were evaluated. Peak stem cell proliferation and morphological changes of adaptation were identified at 7 days. Wnt inhibition diminished IA at 2 wk and resulted in activation of genes of the Wnt/β-catenin and Yes-associated protein (YAP)/Hippo pathway. Increased cytoplasmic YAP was observed in monensin-treated SBS fish. Genes of the WASP-interacting protein (WIP) pathway were elevated during Wnt blockade. In conclusion, cellular proliferation and morphological changes accompany SBS even in attempted Wnt blockade. Wnt/β-catenin, YAP/Hippo pathway, and WIP pathway genes increase during early Wnt blockade. Further understanding of the effects of Wnt and YAP pathway signaling in proliferating stem cells might enrich our knowledge of targets to assist IA. NEW & NOTEWORTHY Intestinal adaptation is a critical response to increase epithelial surface area after large intestinal losses. Inhibition of Wnt/β-catenin signaling impairs intestinal adaptation in a zebrafish model of short bowel syndrome. There is a subsequent upregulation in genes of the Yes-associated protein/Hippo and WIP pathway. These may be targets for future human therapies, as patients are salvaged by the compensation of increased intestinal epithelial surface area through successful intestinal adaptation.
Gait synergetic neuromuscular control in children with cerebral palsy at different gross motor function classification system levels
Authors: Yu Yi , Chen Xiang , Cao Shuai , Wu De , Zhang Xu , Chen Xun 
Published: March 2019

Cerebral palsy (CP) is a neural developmental disease featured with gait abnormalities. CP gait assessment is usually performed with the Gross Motor Function Classification System (GMFCS) in clinics, which does not involve a thorough assessment of neuromuscular control. To understand how the neuromuscular control disorders lead to gait abnormalities, we explored the relationship between GMFCS levels and the gait synergetic control characteristics in this study. In total, 18 children with CP at different GMFCS levels (mean age: 4.41±1.30 yr) and 8 age-matched typically developing (TD) children (mean age: 4.43±1.36 yr) were recruited to perform a straight walking task, and the surface electromyographic (sEMG) signals from eight lower limb muscles on each side and accelerometer data were collected. A nonnegative matrix factorization method was applied to obtain the muscle synergies from the sEMG signals. Next, synergy structures were projected onto the basic gait synergies to test the completeness of those structures. Subsequently, synergy activation parameters, including total activation duration and coactivation index, were compared across the participants. This study showed that children with CP at GMFCS levels I and II and the TD children had similar synergy structures, but the synergy activations of these children with CP were different from those of TD children. In addition, similar to previous research, we also found that children with CP at GMFCS level III could not access all four basic synergies on both sides. Based on the synergy analysis results, a gait assessment paradigm was proposed to facilitate the clinical CP gait evaluation. NEW & NOTEWORTHY Understanding the mechanism of gait abnormality has important clinical significance for the diagnosis, prognosis, and possible treatment of motor dysfunction in children with cerebral palsy (CP). In this study, the comparisons of the lower limb muscle synergies among different groups of children with CP at different Gross Motor Function Classification System levels might provide some new insight into the mechanism underlying the gait disorder. In particular, the discrepancies of gait synergy structure and activation patterns across the study groups may indicate different neurophysiological and pathological attributes in different groups of patients.
Neural Models of Text Normalization for Speech Applications
Authors: Zhang Hao , Sproat Richard , Ng Axel H. , Stahlberg Felix , Peng Xiaochang , Gorman Kyle , Roark Brian 
Published: March 2019

Machine learning, including neural network techniques, have been applied to virtually every domain in natural language processing. One problem that has been somewhat resistant to effective machine learning solutions is text normalization for speech applications such as text-to-speech synthesis (TTS). In this application, one must decide, for example, that 123 is verbalized as one hundred twenty three in 123 pages but as one twenty three in 123 King Ave. For this task, state-of-the-art industrial systems depend heavily on hand-written language-specific grammars. We propose neural network models that treat text normalization for TTS as a sequence-to-sequence problem, in which the input is a text token in context, and the output is the verbalization of that token. We find that the most effective model, in accuracy and efficiency, is one where the sentential context is computed once and the results of that computation are combined with the computation of each token in sequence to compute the verbalization. This model allows for a great deal of flexibility in terms of representing the context, and also allows us to integrate tagging and segmentation into the process. These models perform very well overall, but occasionally they will predict wildly inappropriate verbalizations, such as reading 3 cm as three kilometers. Although rare, such verbalizations are a major issue for TTS applications. We thus use finite-state covering grammars to guide the neural models, either during training and decoding, or just during decoding, away from such “unrecoverable” errors. Such grammars can largely be learned from data.
Contrasting Conditions in the U.K. Winter of 2015/16 as a Result of Remote Tropical Influences
Authors: Maidens Anna , Knight Jeff , Martin Nicola , Andrews Martin 
Published: March 2019

AbstractWinter 2015/16 exhibited contrasting weather patterns in western Europe. Early winter saw a succession of high-impact storms, and December was the wettest calendar month recorded in the United Kingdom. February experienced a shift to relatively cooler conditions. Overall, the winter was well forecast by GloSea5, the Met Office’s seasonal prediction system, capturing the positive North Atlantic Oscillation (NAO) in early winter and the northwesterly pattern of flow in February. In this paper, we use a series of atmospheric relaxation experiments to investigate tropical drivers of these signals and the shift in pressure patterns over the course of the winter. While December’s positive NAO is highly consistent with the strong El Niño event in the tropical Pacific Ocean, this pattern is shown to be modified by wavelike activity from the tropical Atlantic Ocean. The result is a shift in the direction of the prevailing flow from westerly to southwesterly, helping to explain the unusually high U.K. precipitation. In February, the surface pressure pattern is characteristic of a strong (rather than a moderate) El Niño, driven by the eastward extension of the Walker circulation. The relaxation experiments indicate that both the Pacific and Atlantic basins play a role in explaining the phenomena of winter 2015/16. To correctly predict the extratropical response over northern Europe, an accurate representation of tropical forcings in both basins is required.
On the Value of Time-Lag-Ensemble Averaging to Improve Numerical Model Predictions of Aircraft Icing Conditions
Authors: Xu Mei , Thompson Gregory , Adriaansen Daniel R. , Landolt Scott D. 
Published: March 2019

Abstract
The High-Resolution Rapid Refresh (HRRR) model with its hourly updating cycles provides multiple weather forecasts valid at any given time. A logical combination of these individual deterministic forecasts is postulated to show more skill than any single forecast for predicting clouds containing supercooled liquid water (SLW), an aircraft icing threat. To examine the potential value of using multiple HRRR forecasts for icing prediction, a time-lag-ensemble (TLE) averaging method of combining a number of HRRR forecasts was implemented for a multiple month real-time test during the winter of 2016/17. The skills of individual HRRR and HRRR-TLE aircraft icing predictions were evaluated using icing pilot reports (PIREPs) and surface weather observations and compared with the operational Forecast Icing Product (FIP) using the Rapid Refresh (RAP) model. The HRRR-TLE was found to produce a higher capture rate of icing PIREPs and surface icing conditions of freezing drizzle or freezing rain than single deterministic HRRR forecasts. As a trade-off, the volume of airspace warned in HRRR-TLE increased, resulting in a higher false detection rate than in the deterministic HRRR forecasts. Overall, the HRRR-TLE had similar probability of detection and volume of airspace warned for icing as the operational FIP prediction for the icing probability of 25% or greater. Alternative techniques for composing TLE from multiple HRRR forecasts were tested in postseason rerun experiments. The rerun tests also included a comparison of the skills of HRRR and HRRR-TLE to the skills of RAP and RAP-TLE.
Paediatric reference intervals for plasma anti-Müllerian hormone: comparison of data from the Roche Elecsys assay and the Beckman Coulter Access assay using the same cohort of samples
Authors: Yates Allen P , Jopling Helen M , Burgoyne Nicholas J , Hayden Katharine , Chaloner Christopher M , Tetlow Lesley 
Published: March 2019

Background Autoanalyser methods for the measurement of anti-Müllerian hormone have been introduced into clinical laboratories but few reports of paediatric reference intervals using these new assays have been published. Methods After prior evaluation of the Roche Elecsys anti-Müllerian hormone assay against the Beckman Coulter modified second generation anti-Müllerian Hormone enzyme-linked immunosorbent assay using samples from adult females, a cohort of paediatric samples which had previously been assessed using the Beckman Coulter Access anti-Müllerian hormone assay was analysed using the Roche Elecsys anti-Müllerian hormone assay. Results The Roche Elecsys anti-Müllerian hormone assay measured significantly lower than the Beckman Coulter modified second generation anti-Müllerian Hormone enzyme-linked immunosorbent assay. In the paediatric cohort measured with the Roche Elecsys assay, male levels are very high from birth to puberty after which they fall towards postpubertal female levels. Male results were similar to those previously obtained using the Beckman Coulter Access anti-Müllerian hormone assay on the same cohort. Roche Elecsys anti-Müllerian hormone in the females was very low in the neonatal and prepubertal years and the postpubertal trend, with a steady rise from 15 years, was smoother than previously modelled using the Beckman Coulter Access anti-Müllerian hormone assay. Conclusion Anti-Müllerian hormone levels measured with the Roche Elecsys assay were significantly lower than the Beckman Coulter modified second generation enzyme-linked immunosorbent assay suggesting the need for new reference ranges. In the paediatric cohort, Roche Elecsys anti-Müllerian hormone levels between boys and girls showed good prepubertal delineation and small but statistically significant differences to previously measured levels using the Beckman Coulter Access anti-Müllerian hormone assay on the same sample cohort.
Understanding HPV vaccine initiation in Hispanic adolescents using social marketing theory
Authors: Roncancio Angelica M , Muñoz Becky T , Carmack Chakema C , Ward Kristy K , Cano Miguel A , Cribbs Felicity L , Fernandez-Espada Natalie 
Published: March 2019

Objective: Human papillomavirus (HPV) vaccination rates among Hispanic adolescents in the USA are far below recommended levels. Using a social marketing theory framework, we assessed the four Ps (product, price, place and promotion) as they relate to efforts to promote HPV vaccine initiation. Design: Qualitative study involving in-depth interviews. Setting: A large Southern city in the USA. Method: Eighty-five in-depth qualitative interviews were conducted with Spanish-speaking Hispanic mothers divided into initiated and uninitiated market segments based on whether or not their adolescent child had received the first dose of the HPV vaccine. Data were analysed using directed qualitative content analysis. Results: Results suggest that the desire to prevent illnesses and protect their child drives the decision to vaccinate (product). Most initiated mothers did not experience barriers to vaccination, and market segments described different potential barriers (price). Both marker segments reported that they needed vaccine information before vaccinating (price). Finally, interventions should target clinics, schools and community centres to raise awareness about the importance of vaccinating against HPV (place and promotion). Conclusion: Findings from this formative study can inform the development of an intervention to increase HPV vaccine initiation among Hispanic adolescents.
Monitoring of impaired phagocytic function of Kupffer cells in an obstructive cholangitis rat model using superparamagnetic iron oxide MRI and contrast-enhanced ultrasound
Authors: Lee Jae Hwan , Kim Jung Hoon , Lee Seunghyun , Han Joon Koo 
Published: March 2019

Background Kupffer cells (KC) have an important role in the host defense in obstructive cholangitis. Non-invasive monitoring of phagocytic function of KC is pivotal. Several studies showed the possibility of non-invasive monitoring of phagocytic function of KC using superparamagnetic iron oxide-enhanced magnetic resonance imaging (SPIO-MRI) or contrast-enhanced ultrasound (CEUS). Purpose To investigate the serial change of KC function using SPIO-MRI and CEUS and whether the SPIO-MRI parameter correlates with the CEUS parameter in obstructive cholangitis rat models. Material and Methods With our institutional Animal Care and Use Committee approval, 19 rats (common bile duct ligation [CBDL]: n = 9; control: n = 10) underwent SPIO-MRI and CEUS at baseline, two, and four weeks after CBDL. The relative signal loss (RSL) of T2* value on SPIO-MRI and Kupffer phase parenchymal echogenicity (KPE) on CEUS were measured. The correlation between SPIO-MRI and CEUS parameters were compared with KC count. Results In CBDL group, RSL and KPE had significantly decreased (72.1% to 29.5%, 2.7 to 0.4) at four weeks compared with those in the control group (68.2% to 58.3%, 2.5 to 3.0, P < 0.05). During the follow-up period, RSL showed significantly positive correlations with KPE ( P = 0.007). In addition, at four weeks, we found RSL was positively correlated with KPE (ρ = 0.750, P = 0.002). KC count was negatively correlated to RSL and KPE at four weeks (ρ = −0.771, P = 0.001 and ρ = −0.644, P = 0.013). Conclusion SPIO-MRI and CEUS may be equally useful for monitoring the serial changes of KC phagocytic function in vivo.
Side of Hamstring Harvest Does Not Affect Performance, Return-to-Sport Rate, or Future Hamstring Injuries After Ulnar Collateral Ligament Reconstruction Among Professional Baseball Pitchers
Authors: Erickson Brandon J. , Chalmers Peter N. , D’Angelo John , Ma Kevin , Ahmad Christopher S. , Dines Joshua S. , Romeo Anthony A. 
Published: March 2019

Background: Ulnar collateral ligament reconstruction (UCLR) is a successful procedure among professional baseball pitchers. It is unclear if hamstring tendon harvest side for UCLR affects the outcome or alters the risk for subsequent hamstring injury. Hypothesis: Players with prior UCLR with ipsilateral (drive leg) hamstring autograft will have the same return-to-sport (RTS) rate and performance upon RTS but a higher number of subsequent lower extremity injuries than those with contralateral (landing leg) hamstring autograft. Study Design: Cohort study; Level of evidence, 3. Methods: All players between 2010 and 2015 who underwent UCLR with hamstring autograft were included. Surgical details of their procedures were recorded from operative reports. Outcomes for UCLR with hamstring autograft harvested from the drive leg were compared with UCLR with the graft harvested from the landing leg. Results: Overall, 191 players underwent UCLR with hamstring autograft (drive leg, n = 58, 30%; landing leg, n = 133, 70%). The docking technique was more common in the drive leg group, while the figure-of-8 technique was more common in the landing leg group ( P > .001). More patients in the landing leg group underwent concomitant treatment of the ulnar nerve than the drive leg group ( P < .001). No difference existed in RTS rates or timing of RTS between groups. No differences in subsequent ipsilateral or contralateral hamstring injuries occurred between players who underwent UCLR with hamstring autograft from the drive leg or the landing leg ( P≥ .999 and P = .460, respectively). No difference in overall upper or lower extremity injury rates existed between groups (all P > .05), and no difference in performance metrics existed between groups upon RTS. Conclusion: No difference in RTS rate, performance upon RTS, or subsequent injury rates (hamstring, lower extremity, or upper extremity) existed between players who underwent UCLR with hamstring autograft whether the graft came from the drive or the landing leg.
Comment l’autonomie rend-elle une expérience agréable ? Les rôles de la perception du risque et du contrôle personnel
Authors: Lunardo Renaud , Saintives Camille 
Published: March 2019

Résumé Cette recherché remet en cause l’idée selon laquelle l’autonomie est bénéfique pour les consommateurs quelle que soit la situation. Plus précisément, cette recherche démontre, à travers deux expérimentations et une étude terrain, que l’autonomie ne donne lieu à du plaisir que lorsque le risque est faible. Ces études identifient également le contrôle personnel comme un mécanisme expliquant pourquoi l’autonomie rend la consommation plus ou moins agréable, et ce, en fonction de la perception du risque. Dans l’étude 1, nous démontrons que le risque perçu modère l’effet que l’autonomie peut avoir sur le fait de rendre une expérience agréable, l’absence de risque permettant à l’autonomie d’accroître le contrôle personnel. L’étude 2 réplique cet effet modérateur du risque au cours d’une étude terrain. Pour tester si de mêmes effets peuvent être retrouvés avec un autre type de risque perçu, l’étude 3 manipule le risque social et réplique l’effet modérateur du risque dans la relation entre autonomie, contrôle personnel et plaisir observé dans les études 1 et 2. D’une manière générale, cette recherche met en avant la nécessité de prendre en considération le risque lorsqu’on rend les consommateurs autonomes, et offre de nouvelles contributions aux recherches sur l’autonomie des consommateurs.
Performance of SLEDAI-2K to detect a clinically meaningful change in SLE disease activity: a 36–month prospective cohort study of 334 patients
Authors: Jesus D , Rodrigues M , Matos A , Henriques C , Pereira da Silva J A , Inês L S 
Published: March 2019

Objective The objective of this paper is to evaluate the performance of the Systemic Lupus Erythematosus Disease Activity Index (SLEDAI-2K) in detecting clinically meaningful changes in SLE disease activity. Methods A longitudinal cohort study was conducted of 334 SLE patients during a 36-month follow-up. At each outpatient visit, disease activity was scored using the Physician Global Assessment (PGA) and SLEDAI-2K. Correlations between PGA and SLEDAI-2K were assessed. A clinically meaningful change in SLE disease activity was defined as a ΔPGA ≥ 0.3 points from baseline. Performance of SLEDAI-2K in detecting a clinically meaningful worsening or improvement was tested using receiver operating characteristic (ROC) analysis. Results Adjusted mean PGA and SLEDAI-2K scores presented a high correlation (rho = 0.824, p < 0.0005). In ROC analysis, a SLEDAI-2K variation presented an area under the curve (AUC) of 0.697 (95% confidence interval (CI) (0.628–0.766), p < 0.0005) to detect a clinically meaningful improvement, with a sensitivity of 28.8% for a SLEDAI-2K ≥ 4 reduction. The AUC to detect a clinically meaningful worsening was 0.877 (95% CI (0.822–0.932), p < 0.0005), with a sensitivity of 35.3%. Conclusions SLEDAI-2K has a limited ability to detect clinically meaningful changes in SLE disease activity, failing to identify almost two-thirds of cases judged as having a clinically meaningful improvement or worsening. There is a need for more sensitive SLE disease activity measures in clinical practice and research.
A Multidisciplinary Survey to Assess Facilitators and Barriers to Successful Organ Donation in the Intensive Care Unit
Authors: Oczkowski Simon J. W. , Durepos Pamela , Centofanti John , Arsenau Erika , Dhanani Sonny , Cook Deborah J. , Meade Maureen O. 
Published: March 2019

Introduction: Rates of successful organ donation vary between otherwise comparable intensive care units (ICUs). The ICU staff have a unique perspective into the facilitators and barriers underlying this variation in successful deceased organ donation. Research Question: What do ICU staff perceive to be the most meaningful facilitators and barriers to deceased organ donation? Design: We designed and conducted a survey of all disciplines working in the ICU to ascertain the perceived facilitators and barriers to donation in an academic tertiary care hospital. Survey reliability was assessed using Cronbach α. Factor analysis was used to assess construct validity and identify potentially redundant survey items. Results: We had responses from 108 ICU staff, including nurses (n = 75), respiratory therapists (n = 14), physicians (n = 12), chaplains (n = 2), as well as social work, pharmacy, physiotherapy, and occupational therapy (n = 1 each). Perceived facilitators included availability of organ donation organization coordinators, explicit institutional support for donation, ICU staff culture toward donation, standardized order sets for donation, presence of ICU staff with donation experience, and bedside nurse presence at discussions about donation. Perceived barriers included ICU staff ruling out potentially suitable donors before consulting a donor coordinator, physician communication skills, low priority for organ donation among operating room staff, limited family understanding of patient prognosis and organ donation, and limited emotional readiness of families to discuss donation. Discussion: Several staff-perceived facilitators and barriers to deceased organ donation were identified in the ICU. Future research could identify strategies to promote these facilitators and overcome barriers.