bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pilot‐scale Protamex™‐catalysed production of round scad protein hydrolysates:effects of agitation alone and combined with aeration
Authors: Yao Yujing , Yang Juan , Sun‐Waterhouse Dongxiao , Cui Chun , Wang Wei , Dong Keming 
Published: May 2018

SummaryA pilot‐scale production of round scad protein hydrolysates via Protamex™‐catalysed proteolysis was firstly established. Process optimisation then focused on the use of mechanical agitation (MA) alone or combined with aeration (MAA). Comparative examinations revealed that proteolysis involving either MA or MAA was feasible with a hydrolysis degree up to 55%, and individual and total biogenic amine (BA) contents much lower than the commonly recognised limits (including the dominant BA, cadaverine). MAA is more advantageous in suppressing bacterial counts and activities associated with protein decomposition, enabling reduced volatile basic nitrogen and BAs. Thus, additional aeration to mechanical agitation is recommended for industrial proteolysis of raw materials unavoidably containing micro‐organisms (especially anaerobic bacteria) in common handling practice.
Nurses’ confidence in providing and managing care for older persons with depressive symptoms or depression in long‐term care facilities: A national survey
Authors: Chuang Yeu‐Hui , Kuo Li‐Min 
Published: May 2018

AbstractThe prevalence of depressive symptoms among older residents in long‐term care facilities (LTCFs) is high. Nurses are the main healthcare providers in LTCFs and also the persons responsible for detecting changes in residents’ mental function and providing subsequent care. Therefore, it is necessary to understand nurses’ knowledge, attitudes, and confidence regarding care for older residents with depressive symptoms or depression. This study aimed to understand nurses’ level of knowledge of late‐life depression, attitudes towards depression, and confidence levels in caring for older adults with depressive symptoms or depression in LTCFs. A cross‐sectional descriptive and correlational research design was used. A nationwide self‐report survey was conducted in 2016. Ultimately, 556 valid questionnaires were returned. The study found that LTCF nurses’ knowledge about late‐life depression was poor, and they also lacked confidence in managing and caring for older persons with depressive symptoms or depression, but nurses’ attitudes towards depression were neutral or slightly positive. Moreover, nurses who had greater confidence in providing care for older persons with depression were those with more positive attitudes towards depression, a greater interest level in taking care of older adults with depression, less late‐life depression knowledge, longer nursing experience in LTCFs, and a greater interest level in late‐life depression issues, and who had read late‐life depression pamphlets or taken courses or classes in late‐life depression. The findings suggest an urgent need to develop strategies to improve nurses’ late‐life depression knowledge and increase their confidence in providing care to older residents with depressive symptoms or depression.
Blood pressures immediately following ischemic strokes are associated with cerebral perfusion and neurologic function
Authors: He Mingli , Cui Bing , Wu Cunjin , Meng Pin , Wu Taotao , Wang Mingyu , Yang Ru , Zhou Lin , He Xiaobin , Xu Bingchao , Li Zaipo , Xu Bei , Cai Zenglin , Sun Yong’an , Hui Rutai , Wang Yibo 
Published: May 2018

The optimal range of blood pressure levels in the early phase of ischemic stroke with hypertension is still controversial. Based on our stroke registry database, we explored the relationship between blood pressure levels and cerebral perfusion in the early phase of ischemic stroke with hypertension and neurofunctional recovery at 3 months after stroke. Total 732 stroke patients with hypertension were finally analyzed. Patients were divided into quintiles according to systolic blood pressure (SBP) and diastolic blood pressure (DBP) to perform multivariable logistic regression to analyze their relation with neurofunctional recovery, respectively. The cerebral perfusion levels displayed a reverse “U” shape curve with the change of blood pressure levels. Sufficient estimated cerebral blood flow (ECBF) in the early phase of ischemic stroke was associated with good neurofunctional recovery at 3 months after stroke. The best neurofunctional recovery was observed in the middle quintiles with SBP at 161 to 177 mm Hg and DBP at 103 to 114 mm Hg, respectively. So maintaining appropriate blood pressure levels in the early phase of ischemic stroke might be beneficial to cerebral perfusion and neurofunctional recovery.
Primary prevention of chronic kidney disease through population‐based strategies for blood pressure control: The ARIC study
Authors: Hardy Shakia T. , Zeng Donglin , Kshirsagar Abhijit V. , Viera Anthony J. , Avery Christy L. , Heiss Gerardo 
Published: May 2018

While much of the chronic kidney disease (CKD) literature focuses on the role of blood pressure reduction in delaying CKD progression, little is known about the benefits of modest population‐wide decrements in blood pressure on incident CKD. The authors used multivariable linear regression to characterize the impact on incident CKD of two approaches for blood pressure management: (1) a 1‐mm Hg reduction in systolic BP across the entire study population; and (2) a 10% reduction in participants with unaware, untreated, and uncontrolled BP above goal as defined by the Seventh Report of the Joint National Committee on Prevention, Detection, Evaluation, and Treatment of High Blood Pressure (JNC 7) thresholds. Over a mean of 20 years of follow‐up (ARIC [Atherosclerosis Risk in Communities] study, n = 15 390), 3852 incident CKD events were ascertained. After adjustment, a 1‐mm Hg decrement in systolic BP across the population was associated with an estimated 11.7 (95% confidence interval [CI], 6.2–17.3) and 13.4 (95% CI, 10.3–16.6) fewer CKD events per 100 000 person‐years in blacks and whites, respectively. Among participants with BP above JNC 7 goal, a 10% decrease in unaware, untreated, or uncontrolled BP was associated with 3.2 (95% CI, 2.0–4.9), 2.8 (95% CI, 1.8–4.3), and 5.8 (95% CI, 3.6–8.8) fewer CKD events per 100 000 person‐years in blacks and 3.1 (95% CI, 2.3–4.1), 0.7 (95% CI, 0.5–0.9), and 1.0 (95% CI, 1.3–2.4) fewer CKD events per 100 000 person‐years in whites. Modest population‐wide reductions in systolic BP hold potential for the primary prevention of CKD.
Association between functional polymorphisms in IL‐33/ST2 pathway and risk of osteosarcoma
Authors: Wang Jun‐Li , Liu Jia , Xie Ke‐Gong , Lan Chang‐Gong , Lu Lu , Tang Yu‐Jin 
Published: May 2018

AbstractInterleukin (IL)‐33/ST2 pathway plays crucial roles in tumour growth and metastasis. The aim of this study was to investigate the association of two functional polymorphisms (IL‐33 rs7025417 and ST2 rs3821204) with osteosarcoma (OS) risk. The rs7025417 and rs3821204 were genotyped by Taqman assay. IL‐33mRNA and protein levels were measured by real‐time PCR or enzyme‐linked immunosorbent assay. The luciferase activity was measured by a dual luciferase reporter gene assay. The allele‐specific transcription factor binding for rs7025417 was examined by ChIP‐seq. The IL‐33 rs7025417 CC genotype was significantly associated with a decreased risk of OS (CC vs TT: OR = 0.59, 95% CI, 0.41‐0.85; recessive model: OR = 0.68, 95% CI, 0.49‐0.94; C vs T: OR = 0.76, 95% CI, 0.63‐0.91). Combined analysis showed that the IL‐33 rs7025417CT/CC‐ST2 rs3821204CG/CC and the IL‐33 rs7025417CT/CC‐ST2 rs3821204GG genotypes also had a decreased risk of OS. IL‐33mRNA and protein levels in OS patients were significantly higher than controls. Patients with the rs7025417 CC genotype exhibited lower levels of IL‐33 (P = .03). The rs7025417 C allele presented a lower transcriptional activity by disrupting the binding site to c‐Myb (P < .01). Moreover, the rs3821204 G/C influences the transcriptional activity and ST2mRNA expression by altering the binding site of miR‐202‐3p. These findings suggest that the rs7025417 and rs3821204 may have a combined effect to protect against the development of OS by decreasing the expression levels of IL‐33 or ST2.
Efficacy of non‐surgical treatments for androgenetic alopecia: a systematic review and network meta‐analysis
Authors: Gupta A.K. , Mays R.R. , Dotzert M.S. , Versteeg S.G. , Shear N.H. , Piguet V. 
Published: May 2018

AbstractAndrogenetic alopecia, or male/female pattern baldness, is the most common type of progressive hair loss disorder. The aim of this study was to review recent advances in non‐surgical treatments for androgenetic alopecia and identify the most effective treatments. A network meta‐analysis (NMA) was conducted of the available literature of the six most common non‐surgical treatment options for treating androgenetic alopecia in both men and women; dutasteride 0.5 mg, finasteride 1 mg, low‐level laser therapy (LLLT), minoxidil 2%, minoxidil 5% and platelet‐rich plasma (PRP). Seventy‐eight studies met the inclusion criteria, and 22 studies had the data necessary for a network meta‐analysis. Relative effects show LLLT as the superior treatment. Relative effects show PRP, finasteride 1 mg (male), finasteride 1 mg (female), minoxidil 5%, minoxidil 2% and dutasteride (male) are approximately equivalent in mean change hair count following treatment. Minoxidil 5% and minoxidil 2% reported the most drug‐related adverse events (n = 45 and n = 23, respectively). The quality of evidence of minoxidil 2% vs. minoxidil 5% was high; minoxidil 5% vs. placebo was moderate; dutasteride (male) vs. placebo, finasteride (female) vs. placebo, minoxidil 2% vs. placebo and minoxidil 5% vs. LLLT was low; and finasteride (male) vs. placebo, LLLT vs. sham, PRP vs. placebo and finasteride vs. minoxidil 2% was very low. Results of this NMA indicate the emergence of novel, non‐hormonal therapies as effective treatments for hair loss; however, the quality of evidence is generally low. High‐quality randomized controlled trials and head‐to‐head trials are required to support these findings and aid in the development of more standardized protocols, particularly for PRP. Regardless, this analysis may aid physicians in clinical decision‐making and highlight the variety of non‐surgical hair restoration options for patients.
Healthcare Costs for Insured Older U.S. Adults with Hearing Loss
Authors: Simpson Annie N. , Simpson Kit N. , Dubno Judy R. 
Published: May 2018

ObjectivesTo measure 18‐month healthcare cost difference attributable to hearing loss (HL) in older adults.DesignMatched cohort.SettingRetrospective analysis of administrative healthcare bills of insured older Americans.ParticipantsOlder U.S. adults with health insurance in 3 cohorts matched based on HL diagnosis using propensity score methods (N=904,750).MeasurementsComparison groups were defined as those with and without HL diagnosis using International Classification of Diseases, Ninth Revision, diagnosis codes from billing records. Outcomes measured include 18‐month total healthcare payments and healthcare payments broken down according to payment type (inpatient, outpatient, prescription, hearing services). Three comparison cohorts were examined for these outcomes: individuals covered by Medicare plus supplemental private insurance (Cohort 1, n=782,216), those covered only under Medicare (Cohort 2, n=105,296), and those within the Medicare sample that were dually eligible for Medicare and Medicaid coverage (Subcohort 3, n=17,238). The quality of the propensity score match was examined using standardized differences in means or proportions between all matched covariates, and cost outcomes were analyzed using multiple generalized linear regression models.ResultsFully adjusted models showed significantly higher 18‐month healthcare payments for individuals with a diagnosis of HL without indication of use of hearing services than in those without a HL diagnosis in the 3 samples (payment differences: $3,587 Cohort 1, $3,779 Cohort 2, $4,657 Subcohort 3; all p<.001). Payment differences were also found between individuals with HL and indications of hearing services and those without HL.ConclusionWe observed more than 20% higher total healthcare payments over 18 months for a group of insured individuals with HL regardless of insurance type or hearing services use, indicating that negative health‐related effects of HL may increase healthcare use unrelated to HL. Thus, clinical care to ameliorate HL may improve overall health.
Prediction of skin anti‐aging clinical benefits of an association of ingredients from marine and maritime origins: Ex vivo evaluation using a label‐free quantitative proteomic and customized data processing approach
Authors: Hameury Sebastien , Borderie Laurent , Monneuse Jean‐Marc , Skorski Gilbert , Pradines Dominique 
Published: May 2018

SummaryBackgroundThe application of ingredients from marine and maritime origins is increasingly common in skin care products, driven by consumer expectations for natural ingredients. However, these ingredients are typically studied for a few isolated in vitro activities.ObjectivesThe purpose of this study was to carry out a comprehensive evaluation of the activity on the skin of an association of ingredients from marine and maritime origins using label‐free quantitative proteomic analysis, in order to predict the clinical benefits if used in a skin care product.MethodsAn aqueous gel containing 6.1% of ingredients from marine and maritime origins (amino acid‐enriched giant kelp extract, trace element‐enriched seawater, dedifferentiated sea fennel cells) was topically applied on human skin explants. The skin explants’ proteome was analyzed in a label‐free manner by high‐performance liquid nano‐chromatography coupled with tandem mass spectrometry. A specific data processing pipeline (CORAVALID) providing an objective and comprehensive interpretation of the statistically relevant biological activities processed the results.ResultsCompared to untreated skin explants, 64 proteins were significantly regulated by the gel treatment (q‐value ≤ 0.05). Computer data processing revealed an activity of the ingredients on the epidermis and the dermis. These significantly regulated proteins are involved in gene expression, cell survival and metabolism, inflammatory processes, dermal extracellular matrix synthesis, melanogenesis and keratinocyte proliferation, migration, and differentiation.ConclusionsThese results suggest that the tested ingredients could help to preserve a healthy epidermis and dermis, and possibly to prevent the visible signs of skin aging.
Fetal lung size after thoracoamniotic shunting reflects survival in primary fetal hydrothorax with hydrops
Authors: Suyama Fumio , Ozawa Katsusuke , Ogawa Kohei , Sugibayashi Rika , Wada Seiji , Sago Haruhiko 
Published: May 2018

AbstractAimTo assess the role of lung size and abnormal Doppler findings in the umbilical artery (UA) in determining the outcomes of fetuses with primary fetal hydrothorax (FHT) associated with hydrops who underwent thoracoamniotic shunting (TAS).MethodsThis was a retrospective study at a single center. We included cases of primary FHT with hydrops who underwent TAS at our hospital between 2004 and 2016. We assessed the relationship between mortality until 28 days after birth and ultrasound findings, including absent or reversed end‐diastolic velocity (AREDV) in the UA and the lung‐to‐thorax transverse area ratio (LTR), before and after TAS.ResultsForty‐one cases of primary FHT with hydrops underwent TAS. The median (range) gestational age at TAS was 28.5 (19.3–33.8) weeks. Bilateral pleural effusion was observed in 39 cases (95.1%). Among the 41 cases, 19 (46.4%) survived, 11 (26.8%) died in utero, and 11 (26.8%) died in the neonatal period. AREDV in the UA before and after TAS were not associated with mortality (P = 0.32 and 0.47, respectively). The odds ratio for mortality in LTR 0.2–0.3 before TAS was 0.62 (vs LTR < 0.2, P = 0.45) and that in LTR 0.2–0.3 and > 0.3 after TAS were 0.27 and 0.06, respectively (vs LTR < 0.2, P for trend <0.01).ConclusionA larger LTR after TAS was significantly associated with a better prognosis in hydropic primary FHT. The fetal lung size after the procedure may be a prognostic factor of primary FHT.
Comparative Analysis of Markers of Mass Effect after Ischemic Stroke
Authors: Ostwaldt Ann‐Christin , Battey Thomas W.K. , Irvine Hannah J. , Campbell Bruce C.V. , Davis Stephen M. , Donnan Geoffrey A. , Kimberly W. Taylor 
Published: May 2018

ABSTRACTBACKGROUND AND PURPOSEMidline shift determined on magnetic resonance imaging (MRI) or computed tomography (CT) images is a well‐validated marker of mass effect after large hemispheric infarction and associated with mortality. In this study, we targeted a population with moderately sized strokes. We compared midline shift to other imaging markers and determined their ability to predict long‐term outcome.METHODSMRI scans were studied from the Echoplanar Imaging Thrombolysis Evaluation Trial (EPITHET) cohort. Midline shift, acute stroke lesion volume, lesional swelling volume, change in ipsilateral hemisphere volume, the ratio of ipsilateral to contralateral hemisphere volume, and the reduction in lateral ventricle volume were measured. The relationships of these markers with poor outcome (modified Rankin scale score 3–6 at day 90) were assessed. Receiver‐operating characteristic (ROC) curves were generated to compare the performance of each metric.RESULTSOf the 71 included patients, 59.2% had a poor outcome that was associated with significantly larger values for midline shift, lesional swelling volume, and ratio of hemisphere volumes. Lesional swelling volume, change in hemisphere volume, ratio of hemisphere volumes, and lateral ventricle displacement were each correlated with midline shift (Spearman r = .60, .49, .61, and −.56, respectively; all P < .0001). ROC curve analysis showed that lesional swelling volume (area under the curve [AUC] = .791) predicted poor outcome better than midline shift (AUC = .682). For predicting mortality, ROC curve analysis showed that these three markers were equivalent.CONCLUSIONThe ratio of ipsilateral to contralateral hemisphere volume, baseline lesion volume and lesional swelling volume best predicted poor outcome across a spectrum of stroke sizes.
Effects of the Dietary Fermented Tuna By‐product Meal on Growth, Blood Parameters, Nonspecific Immune Response, and Disease Resistance in Juvenile Olive Flounder, Paralichthys olivaceus
Authors: Oncul Fatma O. , Aya Frolan A. , Hamidoghli Ali , Won Seonghun , Lee Geon , Han Kyoung R. , Bai Sungchul C. 
Published: May 2018

AbstractThis study evaluated the effects of dietary fermented tuna by‐product meal (FTBM) in juvenile olive flounder, Paralichthys olivaceus. Five diets were formulated to replace fishmeal (FM) with FTBM at 0% (FTBM0), 12.5% (FTBM12.5), 25.0% (FTBM25), 37.5% (FTBM37.5), or 50% (FTBM50). After 8 wk, weight gain, specific growth rate, and feed efficiency of fish fed FTBM0 and FTBM12.5 diets were significantly higher than fish fed the other diets (P < 0.05). Also, mean cumulative survival rates (%) of fish fed the FTBM0 and FTBM12.5 diets were significantly higher than those fed FTBM50 diet at Day 9 postchallenge with Edwardsiella tarda (P < 0.05). Protein efficiency ratio of fish fed FTBM0 and FTBM12.5 diets was significantly higher (P < 0.05) than fish fed diets FTBM37.5 and FTBM50. Broken‐line regression analysis of weight gain showed an optimal FM replacement level of 10.65% with FTBM. Therefore, the optimal dietary inclusion of FTBM in juvenile olive flounder diets could be greater than 10.65% but less than 12.5% without any adverse physiological effects on fish health.
A high‐resolution anorectal manometry parameter based on integrated pressurized volume: A study based on 204 male patients with constipation and 26 controls
Authors: Seo M. , Joo S. , Jung K. W. , Lee J. , Lee H. J. , Soh J. S. , Yoon I. J. , Koo H. S. , Seo S. Y. , Kim D. , Hwang S. W. , Park S. H. , Yang D.‐H. , Ye B. D. , Byeon J.‐S. , Jung H.‐Y. , Yang S.‐K. , Rao S. S. , Myung S.‐J. 
Published: May 2018

AbstractBackgroundConventional anorectal manometric parameters based on linear waves cannot properly predict balloon expulsion (BE) time. We aimed to determine the correlation between integrated pressurized volume (IPV) parameters during simulated evacuation (SE) and BE time in healthy individuals and constipated patients and to assess the correlation between each parameter and symptoms.MethodsA total of 230 male participants (including 26 healthy volunteers and 204 chronically constipated patients) underwent high‐resolution anorectal manometry (HRAM) and BE tests. The IPV was calculated by multiplying the amplitude, distance, and time from the HRAM profile. Receiver operating characteristic curve (ROC) analysis and partial least square regression (PLSR) were performed.Key ResultsROC analysis indicated that the IPV ratio between the upper 1 cm and lower 4 cm of the anal canal was more effective for predicting BE time (area under the curve [AUC]: 0.74, 95% confidence interval [CI]: 0.67‐0.80, P < .01) than the conventional anorectal parameters, including defecation index and rectoanal gradient (AUC: 0.60, 95% CI: 0.52‐0.67, P = .01). PLSR analysis of a linear combination of IPV parameters yielded an AUC of 0.79. Moreover, the IPV ratio showed a greater clinical correlation with patient symptoms than conventional parameters.Conclusions and InferencesThe IPV parameters and the combination of IPV parameters via PLSR were more significantly correlated with BE time than the conventional parameters. Thus, this study presents a useful diagnostic tool for the evaluation of pathophysiologic abnormalities in dyssynergic defecation using IPV and BE time.
Systemic cytokines are elevated in a subset of patients with irritable bowel syndrome but largely unrelated to symptom characteristics
Authors: Bennet S. M. P. , Palsson O. , Whitehead W. E. , Barrow D. A. , Törnblom H. , Öhman L. , Simrén M. , van Tilburg M. A. L. 
Published: May 2018

AbstractBackgroundSerum levels of pro‐inflammatory cytokines tend to be increased in irritable bowel syndrome (IBS) patients, or subgroups thereof. Still, the link between cytokine levels and IBS symptoms is unclear. We aim to determine systemic cytokine levels in IBS patients and healthy subjects (HS), confirm the presence of a subset of patients with an increased immune activity and to establish if cytokines are linked to IBS symptoms and pathophysiological factors.MethodsSerum levels of interleukin (IL)‐1β, IL‐6, IL‐8, tumor necrosis factor (TNF), and IL‐10 were measured. All subjects reported IBS symptoms using validated questionnaires and underwent colonic sensorimotor testing. Multivariate supervised orthogonal partial least squares‐discriminant analysis (OPLS‐DA) and unsupervised principal component analysis (PCA) and hierarchical cluster analysis (HCA) were implemented.Key ResultsIrritable bowel syndrome patients (n = 246) had higher serum levels of IL‐1β, IL‐6, IL‐8, TNF, and IL‐10 compared to HS (n = 21); however, serum cytokine profiles could not discriminate patients from HS. Moreover, cytokine levels were not correlated with symptoms among patients. Supervised OPLS‐DA identified 104 patients (40% of patients) and unsupervised HCA analysis identified 49 patients (20%) with an increased immune activity indicated by elevated levels of serum cytokines compared to HS and the other patients. However, irrespective of how patients with increased immune activity were identified they were symptomatically similar to patients with no indication of increased immune activity.Conclusions & InferencesSerum cytokines are elevated in IBS patients compared to HS. Immune activation characterizes a subset of patients, but modest associations between cytokine profile and symptoms suggest immune activity does not directly influence symptoms in IBS.