bluuebunny/crossref_metadata_2025_split huggingface dataset:
Trigger warnings in medical student education: A scoping review
Authors: Fisher James , Bennett Jessica , Atkinson Abby , Errington Linda 
Published: November 2024

AbstractBackgroundMedicine is recognised as a challenging course where exposure to potentially distressing content is inevitable. Some educators provide students with warnings before they encounter potentially upsetting content—trigger warnings. In this scoping review, we mapped the existing literature seeking to better understand how trigger warnings are implemented in medical schools and how they are influencing education within them.MethodsBibliographic databases were searched to identify relevant literature, including searching for grey literature. Articles were included if they focussed on medical school education and were written in English. Data analysis was undertaken using both quantitative and qualitative approaches (thematic analysis).FindingsSearching generated 1284 potential records for inclusion. Articles not related to the primary research question were excluded. Abstracts of the remaining 841 articles were screened, and ultimately, 12 articles met criteria for full‐text review. Of these, there were three empirical research studies. Qualitative analysis identified five main themes: purpose, implementation, student distress, responsibility and problems with terminology.ConclusionsThe use of trigger warnings within medical student education remains contentious. There is a lack of consensus as to their purpose and much diversity in how they are implemented. There was limited published empirical evidence to inform practice in this area.
Student professionalism in clerkships
Authors: Cochran Lauren W. , Auerbach Lisa , Roberts Nicole K. 
Published: November 2024

AbstractIntroProfessionalism can be challenging to assess during clerkships. Current literature focuses either on aspirational generalities or professionalism lapses. Most tools assessing professionalism employ Likert scales. This study investigated the frequency and detail with which clerkship directors describe professional behaviours in end‐of‐clerkship narrative assessments.MethodsWe analysed de‐identified core clerkship final narratives from one New York City medical school collected in academic years 2018–2019 and 2019–2020. We used ATLAS.ti and the six‐step thematic approach outlined by Braun & Clarke to perform a qualitative analysis, coding each semantic unit.ResultsWe found that 176 out of 753 narratives (23%) mentioned professionalism, but only 29 (16%) of those provided a detailed description including an anecdote or example. Focusing on these most detailed comments, we identified seven core themes: managing time, following through on expectations, eager to engage, work ethic & efficiency, effective communication, going above and beyond and situational awareness. Themes range from simple behaviours to sophisticated behaviours that may not fully develop until residency.ConclusionNarratives that use the word “professional” or “professionalism” without examples are unlikely to facilitate learning, meaningful assessment or opportunities for remediation. Our hierarchical framework can be used to teach students how to navigate the current clinical environment and can assist supervisors in providing students with specific, actionable feedback and assessment. Our model explicitly fosters a growth mindset in assessing and developing professionalism.
Ancient duplications, multidimensional specializations, and defense role of hexokinases in wheat
Authors: Tian Xiaolin , Li Fan , Lin Jie , Xu Yun , Tian Kai , Gu Lihua , Zhang Yanfeng , Xu Jin‐Rong , Wang Qinhu 
Published: November 2024

SUMMARYHexokinases (HXKs), which sense and catalyze cellular sugar, play a critical role in the growth and development of various plants, including wheat, a primary source of human calories frequently attacked by fungal pathogens. However, the evolutionary dynamics and functional diversification of HXKs in wheat, particularly their roles in plant defense, remain unclear. Here, we discovered that the wheat hexokinase gene family originated through multiple ancient gene duplications across different plant lineages and has undergone comprehensive, multidimensional functional specialization in gene expression, subcellular localization, enzyme activity, and regulation of plant defense responses. Gene expression analysis suggests that two‐thirds of the TaHXK genes are responsive to fungal infection. Subcellular analysis reveals that while six TaHXKs are localized in mitochondria, three TaHXKs from different phylogenetic branches are sorted into other cellular compartments. Notably, biochemical analysis shows that TaHXKs in mitochondria differ in their glucose‐catalyzing activity, with TaHXK5 and TaHXK3 exhibiting the highest and lowest enzyme activity, respectively. Consistently, transient expression analysis suggests that TaHXK5 induces various plant defense responses, while TaHXK3 is defective in activating some plant defense responses. Furthermore, inactivation of the glucokinase activity of TaHXK5 compromised its function in defense activation, suggesting that mitochondrial TaHXKs display functional divergence in both enzyme activity and defense‐inducing activity that are intrinsically connected. Overall, our findings reveal that the multidimensional specialization events following the ancient duplication events may have shaped the functional diversity of HXKs in wheat, shedding light on their evolutionary dynamics and potentially contributing to the improvement of wheat defense.
Oxyntomodulin physiology and its therapeutic development in obesity and associated complications
Authors: Kueh Martin T. W. , Chong Ming Chuen , Miras Alexander D. , le Roux Carel W. 
Published: November 2024

AbstractIncretins, such as glucagon‐like peptide‐1 (GLP1) and glucose‐dependent insulinotropic polypeptide (GIP), have advanced the treatment landscape of obesity to a new pinnacle. As opposed to singular incretin effects, oxyntomodulin (OXM) activates glucagon receptors (GCGR) and glucagon‐like peptide‐1 receptors (GLP1R), demonstrating a more dynamic range of effects that are more likely to align with evolving ‘health gains’ goals in obesity care. Here, we will review the molecular insights from their inception to recent developments and challenges. This review will discuss the physiological actions of OXM, primarily appetite regulation, energy expenditure, and glucose homeostasis. Finally, we will shed light on the development of OXM‐based therapies for obesity and associated complications, and outline important considerations for more translational efforts.
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Trends in Physical Abilities of Physical Education Students Between the Years 2000–2021
Authors: Weissblueth Eyal , Jablonowsky Roni 
Published: November 2024

Background: The comforts of modern life have markedly caused a reduction in physical activity. Approximately only one third of the adult population engages in regular physical activity levels as recommended, resulting in increased morbidity and mortality. The study aimed to determine if physical education undergraduate students’ cardiorespiratory and abdominal endurance levels changed between 2000–2021. Methods: We collected data from 1353 physical education undergraduate students, 20–30 years of age. Data included evaluations of the following abilities: cardiorespiratory endurance using the time result of a 2000-m run, abdominal muscle endurance measured using 1-minute sit-up test results, arms muscle endurance using maximal numbers of push-up test results, and agility using 4 × 10-m shuttle run results. Results: Findings include a statistically significant decline over the years in cardiorespiratory endurance with medium effect size for males and females (r = .293; 95% CI, 0.215 to .367; and r = .222; 95% CI, .133 to .308, respectively), and abdominal muscle endurance with medium effect size for males and females (r = −.394; 95% CI, −.455 to −.329; and r = −.366; 95% CI, −.434 to −.294, respectively). Conclusions: We concluded that the decline in the physical abilities of physical education undergraduate students over a span of 20 years is significant. Also, in light of the current technological age, further physical activity considerations should be given to populations younger than 18 and of other particular adult populations.
Analysis of
Acinetobacter
P-type type IV secretion system-encoding plasmid diversity uncovers extensive secretion system conservation and diverse antibiotic resistance determinants
Authors: Oke Mosopefoluwa T. , Martz Kailey , Mocăniță Mădălina , Knezevic Sara , D'Costa Vanessa M. 
Published: November 2024

ABSTRACT

Acinetobacter baumannii
is globally recognized as a multi-drug-resistant pathogen of critical concern due to its capacity for horizontal gene transfer and resistance to antibiotics. Phylogenetically diverse
Acinetobacter
species mediate human infection, including many considered as important emerging pathogens. While globally recognized as a pathogen of concern, pathogenesis mechanisms are poorly understood. P-type type IV secretion systems (T4SSs) represent important drivers of pathogen evolution, responsible for horizontal gene transfer and secretion of proteins that mediate host-pathogen interactions, contributing to pathogen survival, antibiotic resistance, virulence, and biofilm formation. Genes encoding a P-type T4SS were previously identified on plasmids harboring the carbapenemase gene

bla
NDM-1

in several clinically problematic
Acinetobacter
; however, their prevalence among the genus, geographical distribution, the conservation of T4SS proteins, and full capacity for resistance genes remain unclear. Using systematic analyses, we show that these plasmids belong to a group of 53 P-type T4SS-encoding plasmids in 20 established
Acinetobacter
species, the majority of clinical relevance, including diverse
A. baumannii
sequence types and one strain of
Providencia rettgeri
. The strains were globally distributed in 14 countries spanning five continents, and the conjugative operon’s T4SS proteins were highly conserved in most plasmids. A high proportion of plasmids harbored resistance genes, with 17 different genes spanning seven drug classes. Collectively, this demonstrates that P-type T4SS-encoding plasmids are more widespread among the
Acinetobacter
genus than previously anticipated, including strains of both clinical and environmental importance. This research provides insight into the spread of resistance genes among
Acinetobacter
and highlights a group of plasmids of importance for future surveillance.
Establishment of CD1b-restricted immunity to lipid antigens in the pulmonary response to
Mycobacterium tuberculosis
infection
Authors: Harris Macallister C. , Gary Hadley E. , Cooper Sarah K. , Ackart David F. , DiLisio James E. , Basaraba Randall J. , Cheng Tan-Yun , van Rhijn Ildiko , Branch Moody D. , Podell Brendan K. 
Published: November 2024

ABSTRACT

CD1 is an antigen-presenting glycoprotein homologous to MHC I; however, CD1 proteins present lipid rather than peptide antigens. CD1 proteins are well established to present lipid antigens of
Mycobacterium tuberculosis
(Mtb) to T cells, but understanding the role of CD1-restricted immunity
in vivo
in response to Mtb infection has been limited by the availability of animal models naturally expressing the CD1 proteins implicated in human response: CD1a, CD1b, and CD1c. Guinea pigs, in contrast to other rodent models, express four CD1b orthologs, and here we utilize the guinea pig to establish the kinetics of gene and protein expression of CD1b orthologs, as well as the Mtb lipid-antigen and CD1b-restricted immune response at the tissue level over the course of Mtb infection. Our results indicate transient upregulation of CD1b expression during the effector phase of adaptive immunity that wanes with disease chronicity. Gene expression indicates that the upregulation of CD1b is the result of transcriptional induction across all CD1b orthologs. We show high CD1b3 expression on B cells, and identify CD1b3 as the predominant CD1b ortholog in pulmonary granuloma lesions. We identify
ex vivo
cytotoxic activity directed against CD1b that parallels the kinetic changes in CD1b expression in Mtb-infected lungs and spleen. This study confirms that CD1b expression is modulated by Mtb infection in lung and spleen, leading to pulmonary and extrapulmonary CD1b-restricted immunity as a component of the antigen-specific response to Mtb infection.
Beyond boundaries: exploring a generative artificial intelligence assignment in graduate, online science courses
Authors: Ganjoo Rohini , Rankin James , Lee Benjamin , Schwartz Lisa 
Published: November 2024

ABSTRACT

Generative artificial intelligence (GAI) offers increased accessibility and personalized learning, though the potential for inaccuracies, biases, and unethical use is concerning. We present a newly developed research paper assignment that required students to utilize GAI. The assignment was implemented within three online, asynchronous graduate courses for medical laboratory sciences. Student learning was assessed using a rubric, which rated students’ effective integration and evaluation of GAI-generated content against peer-reviewed research articles, thus demonstrating their critical thinking and synthesis skills, among other metrics. Overall rubric scores were high, suggesting that learning outcomes were met. After field testing, we administered a 16-item survey about GAI utilization, contribution to learning, and ethical concerns. Data (
n
= 32) were analyzed, and free-response answers were thematically coded. While 93.8% of respondents found the GAI-generated content to be “very good” or “excellent,” 28.1% found inaccuracies, and 68.8% “strongly agreed” or “agreed” that GAI should be allowed to be used as a tool to complete academic assignments. Interestingly, however, only 28.1% “strongly agreed” or “agreed” that GAI may be used for assignments if not explicitly authorized by the instructor. Though GAI allowed for more efficient completion of the project and better understanding of the topic, students noted concerns about academic integrity and the lack of citations in GAI responses. The assignment can easily be modified for different learning preferences and course environments. Raising awareness among students and faculty about the ethical use and limitations of GAI is crucial in today’s evolving pedagogical landscape.
Visualizing the virus world inside the cell by cryo-electron tomography
Authors: Zhou Qunfei , Lok Shee-Mei 
Published: November 2024

ABSTRACT

Structural studies on purified virus have revealed intricate architectures, but there is little structural information on how viruses interact with host cells
in situ
. Cryo-focused ion beam (FIB) milling and cryo-electron tomography (cryo-ET) have emerged as revolutionary tools in structural biology to visualize the dynamic conformational of viral particles and their interactions with host factors within infected cells. Here, we review the state-of-the-art cryo-ET technique for
in situ
viral structure studies and highlight exemplary studies that showcase the remarkable capabilities of cryo-ET in capturing the dynamic virus–host interaction, advancing our understanding of viral infection and pathogenesis.
E2F3-dependent activation of FAM111B restricts mouse cytomegalovirus replication in primate cells
Authors: Ostermann Eleonore , Luoto Laura-Marie , Clausen Michaela , Virdi Sanamjeet , Brune Wolfram 
Published: November 2024

ABSTRACT

Cytomegaloviruses are highly species-specific as they replicate only in cells of their own or a closely related species. For instance, human cytomegalovirus cannot replicate in rodent cells, and mouse cytomegalovirus (MCMV) cannot replicate in human and monkey cells. However, the mechanisms underlying the host species restriction remain poorly understood. We have previously shown that passaging MCMV in human retinal pigment epithelial cells allows the virus to replicate to high titers in these cells due to the accumulation of adaptive mutations, such as loss-of-function mutations in the viral M117 gene. The M117 protein interacts with E2F transcription factors and activates E2F-dependent transcription. Here, we show that activation of E2F3 is primarily responsible for MCMV’s inability to replicate in human cells. By transcriptome analysis, we identified two E2F3-induced serine proteases, FAM111A and FAM111B, as potential host restriction factors. By using shRNA-mediated gene knockdown and CRISPR/Cas9-mediated gene knockout, we demonstrated that FAM111B, but not its paralog FAM111A, suppresses MCMV replication in human and rhesus macaque cells. By immunofluorescence, we detected FAM111B predominantly in the nucleus of infected cells with enrichment in viral replication compartments, suggesting that it might play a role during viral replication. The fact that the FAM111B gene is conserved in primates but absent in rodents suggests that MCMV has not evolved to evade or counteract this restriction factor, which is not present in its natural host.

IMPORTANCE
Viruses must counteract host cell defenses to facilitate viral replication. Viruses with a narrow host range, such as the cytomegaloviruses, are unable to counteract cellular defenses in cells of a foreign species. However, little is known about the cellular host range factors restricting cytomegalovirus replication. Here, we show that mouse cytomegalovirus (MCMV) induces the expression of the FAM111 proteases and that FAM111B, but not FAM111A that has previously been shown to restrict the replication of polyomavirus and orthopoxvirus host range mutants, acts as a cellular factor suppressing MCMV replication in human and rhesus monkey cells. The identification of FAM111B as a host range factor should provide new insight into the physiological functions of this poorly characterized protein.
Associations of pulmonary microvascular blood volume with per cent emphysema and CT emphysema subtypes in the community: the MESA Lung study
Authors: Hermann Emilia A , Motahari Amin , Hoffman Eric A , Sun Yifei , Allen Norrina , Angelini Elsa D , Bertoni Alain G , Bluemke David A , Gerard Sarah E , Guo Junfeng , Kaczka David W , Laine Andrew , Michos Erin , Nagpal Prashant , Pankow James S , Sack Coralynn S , Smith Benjamin , Stukovsky Karen Hinckley , Watson Karol E , Wysoczanski Artur , Barr R Graham 
Published: November 2024

BackgroundPulmonary microvasculature alterations are implicated in emphysema pathogenesis, but the association between pulmonary microvascular blood volume (PMBV) and emphysema has not been directly assessed at scale, and prior studies have used non-specific measures of emphysema.MethodsThe Multi-Ethnic Study of Atherosclerosis Lung Study invited participants recruited from the community without renal impairment to undergo contrast-enhanced dual-energy CT. Pulmonary blood volume was calculated by material decomposition; PMBV was defined as blood volume in the peripheral 2 cm of the lung. Non-contrast CT was acquired to assess per cent emphysema and novel CT emphysema subtypes, which include the diffuse emphysema subtype and small-airways-related combined bronchitic-apical emphysema subtype. Generalised linear regression models included age, sex, race/ethnicity, body size, smoking, total lung volume and small airway count.ResultsAmong 495 participants, 53% were never-smokers and the race/ethnic distribution was 35% white, 31% black, 15% Hispanic and 18% Asian. Mean PMBV was 352±120 mL; mean per cent emphysema was 4.95±4.75%. Lower PMBV was associated with greater per cent emphysema (−0.90% per 100 mL PMBV, 95% CI: −1.29 to –0.51). The association was of larger magnitude in participants with 10 or more pack-years smoking and airflow obstruction, but present among participants with no smoking history or airflow limitation, and was specific to the diffuse CT emphysema subtype (−1.48% per 100 mL PMBV, 95% CI: −2.31 to –0.55).ConclusionIn this community-based study, lower PMBV was associated with greater per cent emphysema, including in participants without a smoking history or airflow limitation, and was specific to the diffuse CT emphysema subtype.
Riemann surfaces and winding numbers of Rényi phase structure of charged-flat black holes
Authors: Barzi F. , Moumni H. El , Masmar K. 
Published: November 2024

AbstractIt’s widely recognized that the free energy landscape captures the essentials of thermodynamic phase transitions. In this work, we extend the findings of [1] by incorporating the nonextensive nature of black hole entropy. Specifically, the connection between black hole phase transitions and the winding number of Riemann surfaces derived through complex analysis is extended to the Rényi entropy framework. This new geometrical and nonextensive formalism is employed to predict the phase portraits of charged-flat black holes within both the canonical and grand canonical ensembles. Furthermore, we elucidate novel relations between the number of sheets comprising the Riemann surface of the Hawking–Page and Van der Waals transitions and the dimensionality of black hole spacetimes. Notably, these new numbers are consistent with those found for charged-AdS black holes in Gibbs–Boltzmann statistics, providing another significant example of the potential connection between the cosmological constant and the nonextensive Rényi parameter.
Integrated Analysis of Utilization Efficiency of Intelligent Sports Venues Based on Data Mining Algorithms
Authors: Li Guyue 
Published: November 2024

With the rapid development of information technology and the increasing prosperity of the sports industry, the construction and management of intelligent sports venues have become an important way to improve the efficiency of sports resource utilization and meet diverse fitness needs. This paper focuses on the integrated analysis of the utilization efficiency of intelligent sports venues based on data mining algorithms. Aiming to reveal the inherent laws and potential value in the operation of sports venues through advanced data processing and analysis techniques, and provide a scientific basis for optimizing resource allocation and improving service quality. Intelligent sports venues utilize modern information technologies such as the Internet of Things, big data, and cloud computing to achieve comprehensive and intelligent monitoring and management of venue facilities, personnel activities, environmental conditions, and more. This new management model not only improves the operational efficiency of the venue but also enhances the user experience and meets personalized and diverse fitness needs. This paper focuses on the current situation of the opening of university sports venues to the outside world, aiming to reveal the pain points and bottlenecks in their operation process through detailed investigation and analysis. This study introduces advanced concepts of middleware architecture and constructs a multi-level data integration framework that includes local models, global models, user models, and mapping rules. This framework can effectively integrate distributed heterogeneous data sources, achieving seamless integration and efficient interaction of data. At the same time, with the help of distributed programming technology in cloud computing, it is possible to conduct in-depth mining and analysis of integrated massive data, discover potential value, and provide a scientific basis for decision support.