bluuebunny/crossref_metadata_2025_split huggingface dataset:
Where has the ‘bio’ in bio-psycho-social gone?
Authors: Schweinhardt Petra 
Published: March 2019

Purpose of review
Current definitions of pain do not necessitate tissue damage. This is important because it does justice to the pain patient in whom a nociceptive source is not detectable. However, in conjunction with exciting findings regarding supraspinal pain modulation and a (perceived) failure of identifying nociceptive sources in individual patients, this might have led to a devaluation of the role of nociception for chronic pain. In this review, the relative importance of nociception versus psychological factors for chronic pain is examined by scrutinizing the example of pain present several months following surgical joint replacement for severe osteoarthritis.

Recent findings
In most patients with chronic pain due to severe osteoarthritis, removal of the putative nociceptive source leads to pain elimination/reduction, indicating that their pain depended on nociceptive input. Furthermore, the influence of psychological factors on outcomes following joint replacement for severe osteoarthritis is limited: pain catastrophizing, which is the most consistently identified psychological factor influencing outcome, explains less than 10% of the variance of pain magnitude several months after knee replacement. The influence of psychological factors might be larger for pain disability than for pain magnitude, which could skew the perception of the importance of psychological factors.

Summary
It appears that the importance of nociception relative to psychological factors is often underestimated, at least in the instance of pain present several months following surgical joint replacement for severe osteoarthritis. Because this might apply also to other chronic pain patients, in particular those without disability, research should not neglect the investigation of nociceptive mechanisms, in particular how they might be detected clinically.
Cognitive Problems in Parkinson Disease: Perspectives and Priorities of Patients and Care Partners
Authors: Raein Katrina L. , Ortiz-Hernández Samia , Benge Jared F. 
Published: March 2019

Objective:
To report how people with Parkinson disease (pwPD) and their care partners (CPs) describe the cognitive impacts of the disease, explore the convergent validity of subjective cognitive complaints (SCCs) with measures of cognition and daily functioning, and report the cognitive treatment priorities of pwPD and their CPs.

Background:
Cognitive symptoms in pwPD are common and disabling. Although objective cognitive impairments have been closely studied, SCCs are less well understood.

Methods:
Fifty dyads consisting of a person with PD and his or her CP independently completed a questionnaire that describes cognitive difficulties and was derived from a prior focus group study. Each participant rated the person with PD’s degree of difficulty with symptoms and identified the top five items that would be important treatment targets. Each person with PD also completed the Montreal Cognitive Assessment (MoCA), and his or her CP completed questionnaires assessing the patient’s daily functioning and the CP’s distress.

Results:
Significant correlations existed between CP-reported cognitive symptoms and objective cognitive impairment as assessed by the MoCA. Both patient- and CP-reported SCCs were correlated with the pwPD’s cognition as assessed by the MoCA, with an increasing number of SCCs reported with declining cognition. In general, the pwPD self-reported more SCCs than did the CPs, but for patients with dementia, the CPs reported more SCCs. Language and decision-making were the top treatment priorities.

Conclusions:
In view of the array of cognitive impacts of PD, clinicians and researchers must consider both the reporter (patient or CP) and the overall stage of a patient’s cognitive decline when evaluating SCCs.
Why Sex Matters: A Cognitive Study of People With Multiple Sclerosis
Authors: Donaldson Emily , Patel Viral P. , Shammi Prathiba , Feinstein Anthony 
Published: March 2019

Background:
Cognitive dysfunction affects 40% to 70% of people with multiple sclerosis (MS). Sex may influence a person’s cognition. Although a few studies have reported greater cognitive deficits in men than women, it is unclear whether specific cognitive domains are more vulnerable than others to the effects of sex or whether cognition is influenced by neurologic or psychiatric variables.

Methods:
A chart review was undertaken of 408 people with MS referred to neuropsychological services. Demographic and MS-related variables were extracted from the patients’ records. We used the Minimal Assessment of Cognitive Functioning in Multiple Sclerosis for the neuropsychological assessment. Raw test scores were converted to z scores using Canadian regression-based normative means. A general linear model was conducted on the adjusted scores, controlling for age; years of education; disease course; illness duration; and disability, anxiety, and depression scores.

Results:
Men were more likely than women to have primary progressive MS (χ2=6.415, P=0.011). There were no other sex differences with respect to demographic, neurologic, or psychiatric data. Women performed significantly better than men on the California Verbal Learning Test—Second Edition Total Learning index (F=7.846, P=0.006).

Conclusions:
An analysis of a large, consecutive sample of people with MS demonstrated that sex, independent of demographic, neurologic, or psychiatric factors, is an important determinant in cognitive impairment, with men being more impaired than women on tests of verbal learning and memory.
Dietary intake regulates the circulating inflammatory monocyte pool
Authors: Jordan Stefan , Tung Navpreet , Casanova-Acebes Maria , Chang Christie , Cantoni Claudia , Zhang Dachuan , Wirtz Theresa H. , Naik Shruti , Rose Samuel A. , Brocker Chad N. , Gainullina Anastasiia , Maier Barbara B. , LeRoith Derek , Gonzalez Frank J. , Meissner Felix , Ochando Jordi , Rahman Adeeb , Chipuk Jerry E. , Artyomov Maxim N. , Frenette Paul S. , Piccio Laura , Berres Marie-Luise , Gallagher Emily J. , Merad Miriam 
Published: March 2019

SUMMARYCaloric restriction is known to improve inflammatory and autoimmune diseases. However, the mechanisms by which reduced caloric intake modulates inflammation are poorly understood. Here we show that short-term fasting reduced monocyte metabolic and inflammatory activity and drastically reduced the number of circulating monocytes. Regulation of peripheral monocyte numbers was dependent on dietary glucose and protein levels. Specifically, we found that activation of the low-energy sensor 5’-AMP-activated protein kinase (AMPK) in hepatocytes and suppression of systemic CCL2 production by peroxisome proliferator-activator receptor alpha (PPARα) reduced monocyte mobilization from the bone marrow. Importantly, while caloric restriction improves chronic inflammatory diseases, fasting did not compromise monocyte emergency mobilization during acute infectious inflammation and tissue repair. These results reveal that caloric intake and liver energy sensors dictate the blood and tissue immune tone and link dietary habits to inflammatory disease outcome.HighlightsFasting reduces the numbers of peripheral pro-inflammatory monocytes in healthy humans and mice.A hepatic AMPK-PPARα energy-sensing axis controls homeostatic monocyte numbers via regulation of steady-state CCL2 production.Fasting reduces monocyte metabolic and inflammatory activity.Fasting improves chronic inflammatory diseases but does not compromise monocyte emergency mobilization during acute infectious inflammation and tissue repair.
Krüppel-like factor 2- induced microRNAs: implications for treatment of pulmonary hypertension
Authors: Sindi Hebah , Russomanno Giusy , Jo Kyeong Beom , Abdul-Salam Vahitha B. , Chaudhry Basma Qazi , Ainscough Alexander J. , Szulcek Robert , Bogaard Harm Jan , Morgan Claire C. , Pullamsetti Soni , Alzaydi Mai , Rhodes Christopher J. , Eichstaedt Christina A. , Grünig Ekkehard , Wilkins Martin R. , Wojciak-Stothard Beata 
Published: March 2019

ABSTRACTFlow-activated transcription factor Krüppel-like factor 2 (KLF2) signaling is compromised in pulmonary arterial hypertension (PAH). We aimed to identify KLF2-induced endothelium-protective exosomal microRNAs of potential therapeutic significance.Eight exosomal microRNAs elevated by KLF2 but reduced in PAH were transfected into human pulmonary artery endothelial cells. Of these, only miR-181a-5p and miR-324-5p had anti-apoptotic, anti-inflammatory and anti-proliferative effect on endothelial cells and reduced proliferation of vascular smooth muscle cells in vitro. RNA sequencing of miRNA-transfected HPAECs revealed reduced expression of multiple genes implicated in vascular remodelling, including ETS-1, NOTCH4, ACTA2, TNF-α, IL-1, MMP10, MAPK and NFATC2. KLF2, miR-181a-5p and miR-324-5p were reduced, while their target genes were elevated in blood-derived endothelial colony forming cells and lung tissues from idiopathic and heritable PAH patients with disabling KLF2 mutation and Sugen/hypoxia mice. Supplementation of miR-181a-5p and miR-324-5p or silencing of their target genes attenuated proliferative and angiogenic responses in endothelial cells from idiopathic PAH and prevented development of pulmonary hypertension in Sugen/hypoxia mice.This study highlights potential therapeutic role of KLF2-induced exosomal microRNAs in PAH.
Arabidopsis S2Lb links AtCOMPASS-like and SDG2 activity in histone H3 Lys-4 trimethylation independently from histone H2B monoubiquitination
Authors: Fiorucci Anne-Sophie , Bourbousse Clara , Concia Lorenzo , Rougée Martin , Deton-Cabanillas Anne-Flore , Zabulon Gérald , Layat Elodie , Latrasse David , Kim SoonKap , Chaumont Nicole , Lombard Bérangère , Stroebel David , Lemoine Sophie , Mohammad Ammara , Blugeon Corinne , Loew Damarys , Bailly Christophe , Bowler Chris , Benhamed Moussa , Barneche Fredy 
Published: March 2019

AbstractThe functional determinants of histone H3 Lys-4 trimethylation (H3K4me3), their potential dependency on histone H2B monoubiquitination (H2Bub) and their contribution to defining transcriptional regimes are poorly defined in plant systems. Unlike inS. cerevisiae, where a single SET1 protein catalyzes H3 Lys-4 trimethylation as part of COMPASS (COMPlex of proteins ASsociated with Set1), inArabidopsis thalianathis activity involves multiple histone methyltransferases (HMTs). Among these, the plant-specific SDG2 (SET DOMAIN GROUP2) has a prominent role. We report that SDG2 co-regulates hundreds of genes with SWD2-like b (S2Lb), a plant ortholog of the Swd2 axillary subunit of yeast COMPASS. S2Lb co-purifies with the AtCOMPASS core subunit WDR5 from a high-molecular weight complex, and both S2Lb and SDG2 directly influence H3K4me3 enrichment over highly transcribed genes.S2Lbknockout triggers pleiotropic developmental phenotypes at the vegetative and reproductive stages, including reduced fertility and seed dormancy. Notwithstanding,s2lbseedlings display little transcriptomic defects as compared to the large repertoire of genes targeted by S2Lb, SDG2 or H3 Lys-4 trimethylation, suggesting that H3K4me3 enrichment is important for optimal gene induction during cellular transitions rather than for determining on/off transcriptional status. Moreover, unlike in budding yeast, most of the S2Lb and H3K4me3 genomic distribution does not rely on a trans-histone crosstalk with histone H2B monoubiquitination. Collectively, this study unveils that the evolutionarily conserved COMPASS-like complex has been coopted by the plant-specific SDG2 HMT and mediates H3K4me3 deposition through an H2Bub-independent pathway in Arabidopsis.
A distinct class of bursting neurons with strong gamma synchronization and stimulus selectivity in monkey V1
Authors: Onorato Irene , Neuenschwander Sergio , Hoy Jennifer , Lima Bruss , Rocha Katia-Simone , Broggini Ana Clara , Uran Cem , Spyropoulos Georgios , Womelsdorf Thilo , Fries Pascal , Niell Cristopher , Singer Wolf , Vinck Martin 
Published: March 2019

AbstractCortical computation depends on interactions between excitatory and inhibitory neurons. The contributions of distinct neuron-types to sensory processing and network synchronization in primate visual-cortex remain largely undetermined. We show that in awake monkey V1, there exists a distinct cell-type (≈30% of neurons) that has narrow-waveform action-potentials, high spontaneous discharge-rates, and fires in high-frequency bursts. These neurons are more stimulus-selective and phase-locked to gamma (30-80Hz) oscillations as compared to other neuron types. Unlike the other neuron-types, their gamma phase-locking is highly predictive of their orientation tuning. We find evidence for strong rhythmic inhibition in these neurons, suggesting that they interact with interneurons to act as excitatory pacemakers for the V1 gamma rhythm. These neurons have not been observed in other primate cortical areas and we find that they are not present in rodent V1. However, they resemble the excitatory “chattering” neurons previously identified by intracellular recordings in cat V1. Given its properties, this neuron type should be pivotal for the encoding and transmission of V1 stimulus information.
Rhythmic chromatin interactions with lamin B1 reflect stochasticity in variable lamina-associated domains during the circadian cycle
Authors: Brunet Annaël , Forsberg Frida , Collas Philippe 
Published: March 2019

AbstractMany mammalian genes exhibit circadian expression patterns concordant with periodic binding of transcription factors, chromatin modifications and chromosomal interactions. Here, we report periodic interactions of chromatin with nuclear lamins, suggesting rhythmic associations with the nuclear lamina. Entrainment of the circadian clock is accompanied in mouse liver by a gain of lamin B1-chromatin interactions, followed by oscillations in these interactions at hundreds of lamina-associated domains (LADs). A subset of these oscillations exhibit distinct 12, 18, 24 or 30-h periodicity in our dataset, and affect one or both LAD borders or entire stand-alone LADs. However, most LADs are conserved during the circadian cycle, and periodic LADs are seldom occurrences rather than dominant features of variable LADs. Periodic LADs display oscillation asynchrony between 5’ and 3’ LAD borders, and are uncoupled from periodic gene expression within or in vicinity of these LADs. Accordingly, periodic genes, including central clock-control genes, are often located megabases away from LADs, suggesting residence in a transcriptionally permissive environment throughout the circadian cycle. Autonomous oscillatory associations of the genome with nuclear lamins provide new evidence for rhythmic spatial chromatin configurations. Nevertheless, our data suggest that periodic LADs reflect stochasticity in lamin-chromatin interactions underlying chromatin dynamics in the liver during the circadian cycle. They also argue that periodic gene expression is by and large not regulated by rhythmic chromatin associations with the nuclear lamina.
Construction and validation of EGFP-expressingStaphylococcus aureusclinical strains for adhesion and internalization assays on epithelial cells
Authors: Charaoui-Boukerzaza Sana , Morgene M. Fedy , Rigaill Josselin , Audoux Estelle , He Zhiguo , Grattard Florence , Carricajo Anne , Pozzetto Bruno , Berthelot Philippe , Botelho-Nevers Elisabeth , Verhoeven Paul O. 
Published: March 2019

ABSTRACTBackgroundStaphylococcus aureusis both a major pathogen and a commensal bacterium in humans. It is able to adhere at the surface of epithelial cells of the anterior nares and can trigger its internalization inside these non-professional phagocytic cells. To better understand the interactions of clinical isolates with keratinocytes in the anterior nares, we developed and validated a one-step protocol expressing enhanced green fluorescent protein (EGFP) inS. aureusclinical strains with the aim to study adhesion to and internalization into mammalian cells.MethodsTwentyS. aureusclinical isolates belonging to clonal complexes 5, 8, 30, 45, 398 were selected for one-step transformation protocol with the EGFP-encoding plasmid pBSU101. EGFP expression was analysed by flow cytometry and confocal microscopy. Wild type and isogenic EGFP-expressing strains were compared for adhesion and internalization levels by using the HaCaT cell model.ResultsTransformation was achieved in all theS. aureusstrains regardless of their genetic background. The flow cytometry analysis showed that the mean proportion of EGFP-expressing bacteria was 97.2% (± 2.1) after 4h of incubation. Adhesion and internalization levels were similar in wild-type and isogenic EGFP-expressingS. aureusstrains. Confocal laser scanning microscopy confirmed that EGFP-expressingS. aureusbacteria could be easily identified inside HaCaT keratinocytes.ConclusionThis study reports an efficient protocol for expressing EGFP inS. aureusclinical strains and demonstrates that these EGFP-expressing strains are suitable for adhesion and internalization assays using HaCaT cells, which allows to perform static and dynamicin vitrostudies ofS. aureuscolonization.
Seroprevalence of zoonotic diseases (leptospirosis and Japanese encephalitis) in swine in ten provinces of Vietnam: a Bayesian approach to estimate prevalence
Authors: Lee Hu Suk , Thanh To Long , Ly Nguyen Khanh , Nguyen-Viet Hung , Thakur Krishna K. , Grace Delia 
Published: March 2019

AbstractBackgroundLeptospirosis is an important zoonotic disease with a global distribution, affecting a wide range of mammalian animals and humans. Japanese encephalitis (JE) virus is the major vector-borne zoonotic disease in the Asia-Pacific region. The main objective of this study was to evaluate the seroprevalence of serovar-specificLeptospiraand JE in swine from 10 provinces in Vietnam.MethodsSamples were initially collected for swine influenza surveillance from March to April 2017 at large-scale farms (with at least 50 sows and/or 250 fattening pigs) with pigs that tested positive for influenza in the previous surveillance period (2015-16).FindingsA total of 2,000 sera samples were analyzed from 10 provinces. Overall, the seroprevalence of leptospirosis was 21.05% (95% CI: 19.28-22.90) using a cut-off titer of ≥ 1:100. The apparent prevalence of JE was 73.45% (95% CI: 71.46-75.37) while the true prevalence was slightly higher (74.46%, 95% credible interval: 73.73-86.41). We found a relatively high presence of leptospirosis and JE in pigs kept on large farms. Prevalence was comparable with other studies suggesting opportunistic testing of samples collected for other surveillance purposes can be a valuable tool to better understand and prevent the potential transmission of these zoonotic diseases from pigs to people in Vietnam.ConclusionOur study provides evidence to veterinarians and animal health professionals for evidence-based practice such as diagnosis, vaccination and zoonotic control. Further investigation into the possible role of different domestic animals, wildlife species or environmental factors is needed to identify the potential risk factors and transmission routes in Vietnam.
Re-evaluation of homozygous lethality for EF1A indel polymorphism inPisaster ochraceus
Authors: Wares John P. , Duffin Paige J. 
Published: March 2019

AbstractA genotypic polymorphism in the sea starPisaster ochraceushas been associated with possible overdominant maintenance of diversity, and subsequent studies of this polymorphism suggested that intermittent disease outbreaks could be a driving factor in this system. However, comparative transcriptomic studies of individuals carrying distinct genotypes at the elongation factor 1-alpha (EF1A) region indicated that the marker was not accurately describing the constitutive differences among individuals. Here we more thoroughly assess this EF1A intron region to better understand how polymorphic diversity could be associated with differential disease outcomes and physiological responses, and find that the underlying genetic model is incorrect. In fact, rather than an instance of homozygous lethality, it is clear that previous genotyping efforts were misled by a PCR artefact. We reanalyze results from two previous studies to show that the effects are not as clear as believed.
Cabbage stem flea beetle’s (Psylliodes chrysocephalaL.) susceptibility to pyrethroids and tolerance to thiacloprid in the Czech Republic
Authors: Stará Jitka , Kocourek František 
Published: March 2019

AbstractThe cabbage stem flea beetle (CSFB),Psylliodes chrysocephala(Coleoptera: Chrysomelidae), has recently become a major pest species in winter oilseed rape in the Czech Republic. The susceptibility of CSFB populations from two localities to six pyrethroids, two neonicotinoids, one organophosphate and one oxadiazine was evaluated in 2015-2018 in glass vial experiments. The susceptibility of CSFB to thiacloprid and thiamethoxam was evaluated in feeding experiment in 2017 and 2018. High susceptibility of CSFB populations tolambda-cyhalothrin, cypermethrin, esfenvalerate,tau-fluvalinate, etofenprox, deltamethrin, chlorpyrifos, indoxacarb and acetamiprid was observed in the glass vial experiments. The LC50 and LC90 data obtained for pyrethroids in these experiments in 2015 represent baseline for CSFB resistance monitoring to pyrethroids in the Czech Republic. High tolerance of CSFB to thiacloprid of CSFB was demonstrated in glass vial and the feeding experiment, too.
Time-series expression profiling of sugarcane leaves infected withPuccinia kuehniireveals an ineffective defense system leading to susceptibility
Authors: Correr Fernando Henrique , Hosaka Guilherme Kenichi , Gomez Sergio Gregorio Perez , Cia Mariana Cicarelli , Vitorello Claudia Barros Monteiro , Camargo Luis Eduardo Aranha , Júnior Nelson Sidnei Massola , Carneiro Monalisa Sampaio , Margarido Gabriel Rodrigues Alves 
Published: March 2019

AbstractPuccinia kuehniiis an obligate biotrophic fungus that infects sugarcane leaves causing a disease called orange rust. It spread out to other countries resulting in reduction of crop yield since its first outbreak in Australia. One of the knowledge gaps of that pathosystem is to understand the molecular mechanisms altered in susceptible plants by the stress induced byP. kuehnii. Here we investigated changes in temporal expression of transcripts in pathways associated with the immune system. To achieve this purpose, we used RNA-Seq to analyze infected leaf samples collected at 0, 12, 24, 48 hours after inoculation (hai), 5 and 12 days after inoculation (dai). Ade novotranscriptome was assembled with preprocessed reads, filtering out potential fungal sequences to focus on plant transcripts. Differential expression analyses of adjacent time points revealed substantial changes at 12, 48 hai and 12 dai, coinciding with the events of spore germination, haustoria post-penetration and post-sporulation. During the first 24 hours, a lack of transcripts involved with resistance mechanisms was revealed by underrepresentation of hypersensitive and defense responses. However, two days after inoculation, upregulation of genes involved with immune response regulation provided evidence of some potential defense response. Events related to biotic stress and phenylpropanoid biosynthesis pathways were predominantly downregulated in the initial time points, but expression was later restored to basal levels. Similar waves of expression were apparent for sets of genes in photosynthesis and oxidative processes, whose initial repression could avoid production of signaling molecules. Their subsequent upregulation possibly restored carbohydrate metabolism, ensuring pathogen nutritional requirements were met. Our results support the hypothesis thatP. kuehniiinitially suppressed sugarcane genes involved in plant defense systems. Late overexpression of specific regulatory pathways also suggests the possibility of an inefficient recognition system by a susceptible sugarcane genotype.