bluuebunny/crossref_metadata_2025_split huggingface dataset:
ОЦЕНКА ГЕНЕТИЧЕСКОГО РАЗНООБРАЗИЯ АЛАТАУСКОЙ, ЧЕРНО-ПЕСТРОЙ И ГОЛШТИНСКОЙ ПОРОД КРУПНОГО РОГАТОГО СКОТА КЫРГЫЗСТАНА ПО ГЕНАМ PRL, GH И PIT-1
Authors: ISAKOVA ZH.T. , BYKOVCHENKO Y.G. , CHEKIROV K.B. , TALAIBEKOVA E.T. , KAINAZAROV M.K. , DUISHEEVA ZH.T. , KURMANBEKOVA G.T. , ALDASHEVA N.M. , ALDASHEV A.A. , KIPEN V.N. 
Published: June 2019

Приведены результаты молекулярно-генетического анализа 3 пород крупного рогатого скота Кыргызстана по генам, детерминирующим полиморфизм пролактина РRL (RsaI, g.35333742C>T), гормона роста GH (AluI, g.48118264G>C) и гипофизарного фактора транскрипции PIT-1 (HinfI, g.35419909T>C). В отношении полиморфизма гена PRL показано, что среди коров алатауской, черно-перстрой и голштинской пород преобладали животные с генотипом PRLAA — 57,6%, 59,3% и 61,5%, соответственно. Особи с генотипом PRLBB встречались с низкой частотой — в пределах 1,69—7,69%. Аллель GHL по полиморфизму гена GH среди исследуемых пород являлся мажорным и варьировал в пределах 83,1—98,1%. По полиморфизму гена PIT-1 наиболее распространены у животных алатауской породы аллель PIT-1B (69,5%) и генотип PIT-1AB (47,5%), у черно-пестрой — аллель PIT-1B (72,2%) и генотип PIT-1AB (55,6%), у голштинской — аллель PIT-1B (76,9%) и генотип PIT-1BB (53,8%). Установлена сочетаемость генотипов по 3 полиморфным сайтам и набольшее разнообразие по ним выявлено у алатауской породы.The results of the genetic identification of three cattle breeds of Kyrgyzstan on the genes determining the polymorphism of prolactin РRL (RsaI, g.35333742C>T), growth hormone GH (AluI, g.48118264G>C) and pituitary transcription factor PIT-1 (HinfI, g.35419909T>C) are presented. The PRLAA genotype prevailed among Alatau, Black-Pied and Holstein cows (57.6%, 59.3% and 61.5% respectively). Cows with the PRLBB genotype were identified with a low frequency (within 1.69-7.69 The GHL allele in the polymorphism of the GH gene among the studied cattle breeds was major and varied within 83.1-98.1%. The PIT-1B allele is common among animals of the Alatau breed with a frequency of 69.5% (genotype PIT-1AB – 47.5%), for Black-Pied – 72.2% (genotype PIT-1AB – 55.6%), for Holstein – 76.9% (genotype PIT-1BB – 53.8%). The compatibility of genotypes for the three polymorphic sites was determined and the greatest diversity of them was found in the Alatau breed cattle. Based on the results of molecular genetic testing, we conducted a comparative analysis of the Alatau, Black-Pied and Holstein breeds of Kyrgyzstan with 13 breeds from other regions for which data were freely available, and the level of genetic similarity was calculated.
The possibilities of the development of functional dairy products
Authors: Zsuzsanna Kárnyáczki  , Béla Béri  , József Fenyvessy  , József Csanádi  
Published: June 2019

Because of our new aged world’s emerging stress, over exhausting and move less lifestyle the pollution, the not appropriate food consuming and the low calories in the consumed food there are more and more the called „civilian” disorders.Many people have diabetes and osteoporosis others fight with lung, cardiovascular system, problems and find cancers of many kinds without age exemptions. There could be a solution in changing lifestyle. In the developed side of the world there are presence of food lines with higher content in nutritious and/or vitamins and fibers such as: kalium, calcium, selen, magnesium, plus it contains less saturated fat. Because of that there is a new word in dietetics as functional foods.Food having more inner content and/or biological values, so healthier, are called functional foods. Those foods components picked carefully for healthiness by modern knowledge of dietetics.One of the best raw materials for functional food is the milk. It is already healthy by itself. The Körös-Maros Biofarm Ltd. has a goal of developing and marketing, health protecting organic functional foods in hungaricum products from cured milk. The Jedlik Ányos project helps the Ltd. to achieve this goal. We just finished an antioxidant test and we check if the antioxidant in present in the milk than we check the final product of the presence of the original antioxidant and the form and amount of it.It had been feeding, for two weeks for three herd of cows selected for age, milk output and consanguinity. All of the three herd had been feeding with basic forage. One of the herds had been getting vitamin-E in the amount of 250 mg/bwkg the other group got licopin in the amount of 200 mg/bwkg once a day each by each orally, the third herd was the control group. After two weeks we toke a sample of every herd were processed the samples into yogurt and cheese. Than we checked the raw milk, the cheese and the yogurt for antioxidant content with HPLC method. The test ended with good results by finding a great amount of antioxidant, in not only the raw milk but also in the final product.
PURCHASE BEHAVIOR OF ORGANIC SEAFOOD CONSUMERS IN VIETNAM
Authors: Duy Binh Tran , Ai Tran Huu 
Published: June 2019

Consumers make buying decisions according to specific market conditions and also to various attribute of the product, namely, the specie, the form, and the place of purchasing, product size and its quality. The results of the consumption surveys reveal that consumers perceive fish as a rather safe food, and also as healthy and nutritious food product but seafood consumption varies quite a lot across Vietnam, not only in relation to frequency of consumption but also by types of fish products and types of species preferred in different provinces. Overall, little is known concerning the attitudes and knowledge among Vietnamese people regarding seafood consumption. Therefore, our research aim was to study the consumption habits and preferences of Vietnamese people regarding seafood on the example of the residents in HCM city. The rates of organic seafood consumption and the consumption frequency increase proportionally to the age of consumers. Most of the respondents (32.16%) consume seafood once a week and they believe they should consume seafood more often. If seafood were more available, many participants (39.45%) state they would consume more seafood
FEATURES OF THE DEVELOPMENT OF FINANCIAL POLICY OF RUSSIAN COMPANIES IN MODERN CONDITIONS
Authors: Keri Igor , Gromova Elena , Sinelschikova Nadezhda 
Published: June 2019

The study includes proposals regarding the development of rational financial policies by Russian companies in modern conditions. The authors have identified the following areas of difficulty: sales, the deficit and availability of financial resources and investment for development and distribution, instability of external conditions.
Changes in price policies, optimization of components and volume of trade receivables accounts are some of the suggested ways of improving a company’s sales. The solution to the lack of financial resources for a company’s investment development can be determined by selecting the more effective forms of financing. The authors point out the limitations of investment activities financing under conditions of import substitution while suggesting a composition of indicators of innovation activity.
The study states that the state faces the challenge of creating favourable conditions for improving the financial stability of companies. The authors suggest measures aimed at perfecting the mechanisms of governmental financial policies.
Translocations of European ground squirrel (Spermophilus citellus) along altitudinal gradient in Bulgaria – an overview
Authors: Koshev Yordan , Kachamakova Maria , Arangelov Simeon , Ragyov Dimitar 
Published: June 2019

The European ground squirrel (Spermophiluscitellus) is a vulnerable species (IUCN) living in open habitats of Central and South-eastern Europe. Translocations (introductions, reintroductions and reinforcements) are commonly used as part of the European ground squirrel (EGS) conservation. There are numerous publications for such activities carried out in Central Europe, but data from South-eastern Europe, where translocations have also been implemented, are still scarce.
The present study summarises the methodologies used in the translocations in Bulgaria and analyses the factors impacting their success. Eight translocations of more than 1730 individuals were performed in the period 2010 to 2018. These included 4 reinforcements, 3 reintroductions and 1 introduction. Two of the translocations are still ongoing. Five of the completed six (83%) translocations were successful, although in two cases the number of individuals was critically low. The relatively higher success in Bulgaria than in Central Europe is probably due to using the gained experience. Most of the translocations (6) used a soft release approach. In 6 cases, the animals settled 100 to 720 metres away from the release site, implying management and protection of suitable habitat beyond the translocation area. In 7 of the translocations, the altitude between the donor colony and the release site varied from 470 to 1320 m which could have a hindering effect on the adaptation of animals due to the specific conditions in the mountains. The main reasons for failure are probably poorly selected and maintained habitats and bad climatic conditions (rainy and cool weather) during the translocation action. European funds are of critical importance for translocations, with only two translocations funded by other sources. Based on the gathered data, the current paper also gives some recommendations for improvement in translocation activities.
Heurts et douleur dans la relation de soin
Authors: Loehlé Christian 
Published: June 2019

Peut-être sommes-nous tous d’accord pour affirmer que notre vulnérabilité, comme nous dit notre auteur, est « inhérente à notre condition d’être humain et indispensable à notre travail », mais nous sommes-nous demandé ce que nous faisions de ces douleurs que pathologies ou transfert n’expliquent pas ? Les accueillons-nous ? Luttons-nous contre elles ? Comment ?… Subtil alliage d’AT et de spiritualité propre à son chemin, Christian Loehle nous guide dans l’exploration de ces douleurs tantôt discrètes, tantôt moins discrètes, dans un chemin passionnant. C’est avec une belle profondeur qu’il nous permet de saisir comment en faire une force tant pour le client que pour nous-mêmes. BE
Packet Warriors: An Academic Mobile Action Game for Promoting OSI Model Concepts to Learners
Authors: Uiphanit Thanakorn , Bhattarakosol Pattarasinee , Suanpong Kwanrat , Iamsupasit Sompoch 
Published: June 2019

<p class="0abstract">This research aims to develop a game to teach the theoretical concepts of the OSI reference model to students who must learn data communication and networking as a compulsory or an elective subject. The survey was administered to most students at Suan Sunandha Rajabhat University by a questionnaire to assess a suitable game for students of this generation. The result showed that action games are the most popular game for them. Then, a script of the OSI reference model was drawn based on the theoretical contents and the software design and implementation were performed. After finishing this development process, 19 third-year undergraduate students majoring in information sciences at Suan Sunandha Rajabhat University tested this system. The evaluation process was performed using pretest and posttest data analyzed by a paired samples t-test at a 95% confidence level. The result of this study shows that the action game ‘Packet Warrior’ is an efficient tool to help students improve their learning capabilities via fun and good relationships with friends. </p>
Gender Differences in Perceptions of Conventional Video, Virtual Reality and Augmented Reality
Authors: Dirin Amir , Alamäki Ari , Suomala Jyrki 
Published: June 2019

<p class="Abstract"><strong>Abstract--</strong>The user’s emotional involvement plays an important role in adopting new technologies. The level of engagement with and adoption of new digital applications depends on various personal, contextual, and emotional factors. In our study, we assessed the personal factors, such as gender differences, of perceiving and adopting technologies such as virtual reality (VR), augmented reality (AR), and conventional video. Furthermore, we assessed what kind of emotions are involved in and invoked by the context of new technologies. This is a quantitative study in which students were asked to perform experiments on VR, AR, and conventional videos. After the experiments, participants were asked to fill out a predefined survey about their emotional reactions to the experiments. The results show, unlike the prior research, that female participants were more enthusiastic about the usage of new technologies than males. The user experience of VR, AR and conventional videos triggered more positive emotions among females than males. For practitioners, the results suggest that the audio-visual technologies could engage more females than males. For academics, this study provides further research on how to trigger users to adopt new audio-visual technologies<em>. </em></p>
Is Dolly Varden in Arctic Alaska Increasing in Length in a Warming Climate?
Authors: Courtney Michael B. , DeSanto Harrison , Seitz Andrew C. 
Published: June 2019

AbstractThe body condition, abundance, and size of several vertebrate taxa occupying the Alaskan and Canadian Arctic have increased in this rapidly changing environment. Presently, anecdotal stakeholder reports suggest that anadromous populations of Dolly Varden Salvelinus malma in Arctic Alaska are attaining greater maximum sizes than reported in the past. However, growth analyses have not been conducted for any substantial period of time. To qualitatively examine one facet of growth, we reviewed scientific journal articles, gray literature, and unpublished data for reported maximum lengths of Dolly Varden from the Chukchi and Beaufort seas collected over the past ∼50 y (1969–2015). Regression analyses of maximum length of Dolly Varden from 1969 to 2015 support the observations that the maximum size of this species is likely increasing in a changing Arctic. These results, coupled with the lack of comprehensive growth data, highlight the importance of long-term monitoring of organismal responses to a changing environment and provide valuable direction for future research on this important subsistence resource for Indigenous peoples who inhabit the Arctic.
The ECMWF operational ensemble reanalysis–analysis system for ocean and sea ice: a description of the system and assessment
Authors: Zuo Hao , Balmaseda Magdalena Alonso , Tietsche Steffen , Mogensen Kristian , Mayer Michael 
Published: June 2019

Abstract. The ECMWF OCEAN5 system is a global ocean and sea-ice ensemble of reanalysis and real-time analysis. This paper gives a full description
of the OCEAN5 system, with the focus on upgrades of system components with
respect to its predecessors, ORAS4 and ORAP5. An important novelty in OCEAN5
is the ensemble generation strategy that includes perturbation of initial
conditions and a generic perturbation scheme for observations and forcing
fields. Other upgrades include revisions to the a priori bias correction
scheme, observation quality control and assimilation method for sea-level
anomalies. The OCEAN5 historical reconstruction of the ocean and sea-ice
state is the ORAS5 reanalysis, which includes five ensemble members and covers
the period from 1979 onwards. Updated versions of observation data sets are
used in ORAS5 production, with special attention devoted to the consistency
of sea surface temperature (SST) and sea-ice observations. Assessment of
ORAS5 through sensitivity experiments suggests that all system components
contribute to an improved fit to observation in reanalyses, with the most
prominent contribution from direct assimilation of ocean in situ
observations. Results of observing system experiments further suggest that
the Argo float is the most influential observation type in our data assimilation
system. Assessment of ORAS5 has also been carried out for several key ocean
state variables and verified against reference climate data sets from the ESA CCI (European Space Agency Climate Change Initiative) project. With respect to ORAS4, ORAS5 has improved ocean climate state and
variability in terms of SST and sea level, mostly due to increased model
resolution and updates in assimilated observation data sets. In spite of the
improvements, ORAS5 still underestimates the temporal variance of sea level and continues exhibiting large SST biases in the Gulf Stream and its extension
regions which are possibly associated with misrepresentation of front
positions. Overall, the SST and sea-ice uncertainties estimated using five
ORAS5 ensemble members have spatial patterns consistent with those of
analysis error. The ensemble spread of sea ice is commensurable with the
sea-ice analysis error. On the contrary, the ensemble spread is
under-dispersive for SST.
Impurity Removal of Waste Cooking Oil Using Hydrophobic Polypropylene Hollow Fiber Membrane
Authors: Aliwarga Lienda , Widodo Setyo , Suwardana Novika , Darmawan Hanna , Khoiruddin Khoiruddin , Wenten I Gede 
Published: June 2019

Removal of impurities from cooking oil is an important step in providing the possibility of WCO reuse to extend the life cycle of cooking oil, leading to a reduction of WCO disposal. This study was conducted to investigate the performance of a polypropylene (PP) hollow fiber ultrafiltration (UF) membrane for removal of impurities from WCO. The results showed that the membrane could remove water content up to 95% (at 0.1 MPa and 30 °C), but the color improvement was only 9.5% as indicated by the absorbance reduction. Within the range of the operation conditions (i.e. a trans-membrane pressure of 0.1-0.2 MPa and a temperature of 30-50 °C), the oil flux varied from 0.3 L.m"‘2.h"‘1 to 1.3 L.m-2.h-1. In long-term operation, the membrane wettability was improved as shown by the oil contact angle decreasing from 28.2 ± 1.5° to 14.4 ± 0.5°. This resulted in a higher oil flux. At the same time, the hydrophobicity was also increased, as indicated by an increase in the water contact angle from 95.4 ± 0.7° to 97.3 ± 1.1°.
Performance of a Multiplex Nested Polymerase Chain Reaction in Detecting 7 Pathogens Containing DNA in Their Genomes Associated With Congenital Infections
Authors: Yamamoto Lidia , Filho Antonio G. Amorim , Queiroz Joelma A. , de Carvalho Mario H. B. , Rodrigues Jonatas C. , Kanunfre Kelly A. , Francisco Rossana P. V. , Okay Thelma Suely 
Published: June 2019

Context.—
Infections are the leading cause of perinatal and infant mortality in low-income and low-resource countries, which have a higher prevalence of infections. Definitive diagnosis of congenital and perinatal infections is largely dependent upon the results of laboratory tests.

Objective.—
To develop a multiplex nested polymerase chain reaction (PCR) technique for the simultaneous detection of 7 pathogens containing DNA in their genomes in suspected cases of congenital infection.

Design.—
Eligible participants were pregnant women with positive immunoglobulin M antibodies raised to one of the pathogens in the prenatal serologic screening, associated or not with fetal ultrasound abnormalities or positive fetal serology. Neonates whose mothers did not attend prenatal care were included when they presented with symptomatology and laboratory parameters suggestive of infection. The detection rate of the multiplex nested PCR was compared with maternal, fetal, and neonatal serology, as well as placental immunohistochemistry and noncommercial amplifications.

Results.—
Of 161 suspected cases, the multiplex nested PCR detected 60 (37.3%), whereas the tests available in hospital laboratories detected 13 of 60 (21.7%) of the cases detected by the multiplex nested PCR, demonstrating a 4.6 times higher detection rate for the multiplex nested PCR (Fisher exact test, P &lt; .001). Positive amplifications were to Toxoplasma gondii (32 cases), cytomegalovirus (14 cases), parvovirus B19 (5 cases), and adenovirus (5 cases). In 4 cases, 2 pathogens were simultaneously detected. All types of biological matrices were suitable for amplification. Sequencing of multiplex nested PCR products confirmed the molecular findings.

Conclusions.—
The multiplex nested PCR significantly increased the number of diagnosed congenital infections. Given the scarcity of DNA recovered from amniotic fluid and some neonatal samples, this multiplex nested PCR allows the simultaneous detection of 7 pathogens associated with congenital infections in a reliable, faster, cost-effective, and more sensitive way.
The complete mitochondrial genome of Paragonimus ohirai (Paragonimidae: Trematoda: Platyhelminthes) and its comparison with P. westermani congeners and other trematodes
Authors: Le Thanh Hoa , Nguyen Khue Thi , Nguyen Nga Thi Bich , Doan Huong Thi Thanh , Agatsuma Takeshi , Blair David 
Published: June 2019

We present the complete mitochondrial genome of Paragonimus ohirai Miyazaki, 1939 and compare its features with those of previously reported mitochondrial genomes of the pathogenic lung-fluke, Paragonimus westermani, and other members of the genus. The circular mitochondrial DNA molecule of the single fully sequenced individual of P. ohirai was 14,818 bp in length, containing 12 protein-coding, two ribosomal RNA and 22 transfer RNA genes. As is common among trematodes, an atp8 gene was absent from the mitogenome of P. ohirai and the 5′ end of nad4 overlapped with the 3′ end of nad4L by 40 bp. Paragonimusohirai and four forms/strains of P. westermani from South Korea and India, exhibited remarkably different base compositions and hence codon usage in protein-coding genes. In the fully sequenced P. ohirai individual, the non-coding region started with two long identical repeats (292 bp each), separated by tRNAGlu. These were followed by an array of six short tandem repeats (STR), 117 bp each. Numbers of the short tandem repeats varied among P. ohirai individuals. A phylogenetic tree inferred from concatenated mitochondrial protein sequences of 50 strains encompassing 42 species of trematodes belonging to 14 families identified a monophyletic Paragonimidae in the class Trematoda. Characterization of additional mitogenomes in the genus Paragonimus will be useful for biomedical studies and development of molecular tools and mitochondrial markers for diagnostic, identification, hybridization and phylogenetic/epidemiological/evolutionary studies.