bluuebunny/crossref_metadata_2025_split huggingface dataset:
Systematic review and meta-analysis of the relationship between sleep disorders and suicidal behaviour in patients with depression
Authors: Wang Xiaofen , Cheng Sixiang , Xu Huilan 
Published: October 2019

Abstract

Background
The potential link between sleep disorders and suicidal behaviour has been the subject of several reviews. We performed this meta-analysis to estimate the overall association between sleep disorders and suicidal behaviour and to identify a more specific relationship in patients with depression.

Methods
A systematic search strategy was developed across the electronic databases PubMed, EMBASE and the Cochrane Library from inception to January 1, 2019 for studies that reported a relationship between sleep disorders and suicidal behaviour in depressed patients. The odds ratio (OR) and corresponding 95% confidence interval (CI) were used to measure the outcomes. Heterogeneity was evaluated by Cochran’s Q test and the I2 statistic. The Newcastle-Ottawa Scale (NOS) was adopted to evaluate the methodological quality of each of the included studies, and the Grading of Recommendations Assessment, Development and Evaluation (GRADE) approach was used to assess the quality of the evidence. We calculated the overall association between sleep disorders and suicidal behaviour and estimated more specific categories, including insomnia, nightmares, hypersomnia, suicidal ideation, suicide attempt, and completed suicide.

Results
A total of 18 studies were included in this study. Overall, sleep disorders were closely related to suicidal behaviour in patients with depression (OR = 2.45 95% CI: 1.33 4.52). The relatively increased risks of sleep disorders with suicidal ideation, suicide attempt and completed suicide ranged from 1.24 (95% CI: 1.00 1.53) to 2.41 (95% CI: 1.45 4.02). Nightmares were found to be highly correlated with the risk of suicidal behaviour (OR = 4.47 95% CI: 2.00 9.97), followed by insomnia (OR = 2.29 95% CI: 1.69 3.10). The certainty of the evidence was rated as very low for the overall outcome and the major depression subgroup and was rated as low for the depression subgroup.

Conclusions
This meta-analysis supports the finding that sleep disorders, particularly nightmares and insomnia, increase the risk of suicidal behaviour in depressed patients. Considering that all included studies were observational, the quality of the evidence is rated as very low. More well-designed studies are needed to confirm our findings and to better explain the mechanisms by which sleep disorders aggravate suicidal behaviour in depressed patients.
Mis-anaesthetized society: expectancies and recreational use of ketamine in Taiwan
Authors: Chang Chao-Ming , Wu Tat Leong , Ting Te-Tien , Chen Chuan-Yu , Su Lien-Wen , Chen Wei J. 
Published: October 2019

Abstract

Background
The popularity of ketamine for recreational use has been increasing in Asia, including Taiwan. Still, little known about the pattern of ketamine expectancies and whether such patterns are related to ketamine use. This study aimed to examine whether the positive and negative ketamine expectancies are differentially associated with ketamine-using behavior, and whether such relationship may differ by early-onset use of tobacco or alcohol.

Methods
Participants were recruited using respondent-driven sampling (RDS) among regular tobacco and alcohol users, aged 18 to 50, residing in Taipei from 2007 to 2010. Totally 1115 participants (with an age distribution skewed to the right, median = 26; interquartile range: 22–32) had information on substance use and completed a 12-item ketamine expectancies questionnaire (with 6 positive and 6 negative statements). Using two axes of High and Low expectancies, the four combinations of binary positive and binary negative ketamine expectancies were created. Each participant’s drug-using experience was categorized into illicit drug naïve, exclusive ketamine use, polydrug ketamine use, or other illicit drug use. Using the weights in the network output by RDS Analysis Tool, multivariable logistic regression analysis was then conducted.

Results
The weighted prevalence was 2.4% for exclusive ketamine use, 9.0% for polydrug ketamine use, and 9.1% for the other illicit drug use. Ketamine users (11.4%) had greater positive expectancies and lower negative expectancies, particularly the combination of High Positive with Low Negative, as compared to the illicit drug-naïve or other illicit drug users. After adjustment for early-onset tobacco (or alcohol) use and sociodemographic characteristics, High Positive, Low Negative, and their combination of High Positive-Low Negative expectancies remained strongly associated with ketamine uses, without evidence of moderation from early-onset use of tobacco or alcohol.

Conclusions
Positive and negative ketamine expectancies were associated in opposite directions with ketamine use, independent of early-onset use of tobacco or alcohol. Our results indicate ketamine expectancies as possible targets for future intervention and prevention of ketamine use, with a less confrontational feedback on decreasing an individual’s positive expectancies is essential in preventing young people from the initiation of ketamine use.
Engaging research ethics committees to develop an ethics and governance framework for best practices in genomic research and biobanking in Africa: the H3Africa model
Authors:   as members of the H3Africa Consortium, Tindana Paulina , Yakubu Aminu , Staunton Ciara , Matimba Alice , Littler Katherine , Madden Ebony , Munung Nchangwi Syntia , de Vries Jantina 
Published: October 2019

Abstract
In the past decade, there has been an increase in genomic research and biobanking activities in Africa. Research initiatives such as the Human Heredity and Health in Africa (H3Africa) Consortium are contributing to the development of scientific capacity and infrastructure to support these studies on the continent. Despite this growth, genomic research and biobanking have raised important ethical challenges for key research stakeholders, including members of research ethics committees. One of these is the limited ethical and regulatory frameworks to guide the review and conduct of genomic studies, particularly in Africa. This paper is a reflection on a series of consultative activities with research ethics committees in Africa which informed the development of an ethics and governance framework for best practices in genomic research and biobanking in Africa. The paper highlights the engagement process and the lessoned learned.
Mosquitoes as a feasible sentinel group for anti-malarial resistance surveillance by Next Generation Sequencing of Plasmodium falciparum
Authors: Smith-Aguasca Rebecca , Gupta Himanshu , Uberegui Estefania , Maquina Mara , Saute Francisco , Paaijmans Krijn P. , Mayor Alfredo , Huijben Silvie 
Published: October 2019

AbstractBackgroundPlasmodium falciparumdrug resistance surveillance is key to successful disease control and eradication. Contemporary methods that only allow determination of prevalence of resistance are expensive, time consuming and require ethical considerations. A newer method involving Next Generation Sequencing (NGS) permits obtaining frequency of resistance while allowing to detect minority variants in mixed infections. Here, NGS was tested forP. falciparumresistance marker detection in mosquito samples as a feasible and suitable alternative for molecular resistance surveillance.Anopheles funestuswere collected in southern Mozambique using CDC light traps and manual collections. DNA was extracted from either whole mosquito, head-thorax and abdomen separately or pools of five mosquitoes. These samples were screened forP. falciparumand if positive fork13,pfcrt,pfmdr1,pfdhpsandpfdhfrmutations related to anti-malarial drug resistance with Sanger sequencing and NGS.ResultsAmong the 846 samples screened forP. falciparum, 122 were positive by 18S ssrDNA qPCR with an infection rate of 23.6%. No mutations were observed fork13andpfcrt72-76 and almost zero forpfmdr86, but quintuplepfdhfr/pfdhpsmutations were near fixation and about half of the isolates contained thepfmdr184F polymorphism. Similar allele frequencies of resistance markers were estimated with NGS in comparison with the prevalence of markers obtained with the gold standard Sanger sequencing.ConclusionsPooled deep sequencing ofP. falciparumisolates extracted from mosquitoes is a promising, efficient and cost-effective method to quantify allele frequencies at population level which allows to detect known and unknown markers of resistance in single and mixed infections in a timelier manner. Using mosquitoes as sentinel group and focusing on allele frequency opposed to prevalence, permits active surveillance across a more homogeneous geographical range.
Effects of transcranial direct current stimulation on patients with disorders of consciousness after traumatic brain injury: study protocol for a randomized, double-blind controlled trial
Authors: Li Shilin , Dong Xiangli , Sun Weiming , Zhao Na , Yu Guohua , Shuai Lang 
Published: October 2019

AbstractBackgroundDisorders of consciousness (DOC) after traumatic brain injury (TBI) raise the mortality of patients, restrict the rehabilitation of patients with TBI, and increase the physical and economic burden that TBI imposes on patients and their families. Thus, treatment to promote early awakening in DOC after TBI is of vital importance. Various treatments have been reported, but there is no advanced evidence base to support them. Transcranial direct current stimulation (tDCS) has shown great potential in promoting neuroelectrochemical effects. This protocol is for a double-blind, randomized, controlled, clinical trial aiming to research the effects and safety of conventional rehabilitation combined with tDCS therapy in patients with DOC after TBI.Methods/designEighty patients with DOC after TBI will be randomized into one of two groups receiving conventional rehabilitation combined with sham tDCS or conventional rehabilitation combined with active tDCS. The intervention period in each of the two groups will last 4 weeks (20 min per day, 6 days per week). Primary outcomes (Glasgow Outcome Scale (GOS)) will be measured at baseline and the end of every week from the first to the fourth week. Secondary outcomes will be measured at baseline and the end of the fourth week. Adverse events and untoward effects will be measured during each treatment.DiscussionPatients with central nervous system lesions have received tDCS as a painless, non-invasive, easily applied and effective therapy for several decades, and there has been some evidence in recent years showing partial improvement on the level of consciousness of partial patients with DOC. However, reports mainly focus on the patients in a minimally conscious state (MCS), and there is a lack of large-sample clinical trials. This protocol presents an objective design for a randomized controlled trial that aims to study the effectiveness of conventional rehabilitation combined with tDCS therapy for DOC after TBI, to evaluate its safety, and to explore effective and economical therapeutic methods.Trial registrationChinese Clinical Trial Registry,ChiCTR1800014808. Registered on 7 February 2018.
The complete mitochondrial genome and description of a new cryptic species of Benedenia Diesing, 1858 (Monogenea: Capsalidae), a major pathogen infecting the yellowtail kingfish Seriola lalandi Valenciennes in the South-East Pacific
Authors: Baeza J. Antonio , Sepúlveda Fabiola A. , González M. Teresa 
Published: October 2019

Abstract

Background
The monogenean Benedenia seriolae parasitizes fishes belonging to the genus Seriola, represents a species complex, and causes substantial impact on fish welfare in aquaculture systems worldwide. This study reports, for the first time, the complete mitochondrial genome of B. humboldti n. sp., a new cryptic species from the South-East Pacific (SEP).

Methods
The mitogenome of B. humboldti n. sp. was assembled from short Illumina 150 bp pair-end reads. The phylogenetic position of B. humboldti n. sp. among other closely related congeneric and confamiliar capsalids was examined using mitochondrial protein-coding genes (PCGs). Morphology of B. humboldti n. sp. was examined based on fixed and stained specimens.

Results
The AT-rich mitochondrial genome of B. humboldti is 13,455 bp in length and comprises 12 PCGs (atp8 was absent as in other monogenean genomes), 2 ribosomal RNA genes, and 22 transfer RNA genes. All protein-coding, ribosomal RNA, and transfer RNA genes are encoded on the H-strand. The gene order observed in the mitochondrial genome of B. humboldti n. sp. was identical to that of B. seriolae from Japan but different from that of B. seriolae from Australia. The genetic distance between B. humboldti n. sp. and B. seriolae from Japan was high. Minor but reliable differences in the shape of the penis were observed between Benedenia humboldti n. sp. and congeneric species.

Conclusions
Phylogenetic analyses based on PCGs in association with differences in the shape of the penis permitted us to conclude that the material from the South-East Pacific represents a new species of Benedenia infecting S. lalandi off the coast of Chile. The discovery of this parasite represents the first step to improving our understanding of infestation dynamics and to develop control strategies for this pathogen infecting the farmed yellowtail kingfish, Seriola lalandi, in the South-East Pacific.
Status of perceived social support and its associated factors among inmate prisoners in Northwest Amhara, Ethiopia
Authors: Dadi Abel Fekadu , Dachew Berihun Assefa , Tariku Amare , Habitu Yohannes Ayanaw , Demissie Getu Debalkie 
Published: October 2019

Abstract

Objective
The objective of this study was to determine the level of social support and associated factors in selected prison institutions in Amhara region, Ethiopia.

Result
Prisoners that had good social support from their family, friends, and significant others were 64.7%, (95% CI 60.9%, 68.4%). The odds of social support was higher among those educated and rural prisoners. However, it was found to be lower among non-Orthodox Christian prisoners and prisoners who were discriminated. Social support is buffering tool for social difficulties and hardships faced by prisoners while they are in prison and very helpful to reduce mental health morbidities and their consequences, hence should be strengthened.
Circulating adult stem and progenitor cell numbers—can results be trusted?
Authors: Kröpfl Julia M. , Schmid Michelle , Di Marzio Yvonne , Schreiber Karine , Spengler Christina M. 
Published: October 2019

Abstract

Background
Within the last years, the interest in physical exercise as non-invasive stimulus influencing circulating hematopoietic stem and progenitor cell (CPC) concentrations has constantly grown. Cell estimates are often derived by determining the subgroup of CPC as percent lymphocytes (LYM) or mononuclear cells (MNC) via flow cytometry and back calculation over whole blood (WB) cell counts. However, results might depend on the used cell isolation technique and/or gating strategy. We aimed to investigate MNC loss and apoptosis during the flow cytometry sample preparation process preceded by either density gradient centrifugation (DGC) or red blood cell lysis (RBCL) and the potential difference between results derived from back calculation at different stages of cell isolation and from WB.

Methods
Human blood was subjected to DGC and RBCL. Samples were stained for flow cytometry analysis of CPC (CD34+/CD45dim) and apoptosis analysis (Annexin V) of MNC and CPC subsets. MNC and LYM gating strategies were compared.

Results
Both DGC as well as RBCL yielded comparable CPC concentrations independent of the gating strategy when back calculated over WB values. However, cell loss and apoptosis differed between techniques, where after DGC LYM, and monocyte (MONO) concentrations significantly decreased (p < 0.01 and p < 0.05, respectively), while after RBCL LYM concentrations significantly decreased (p < 0.05) and MONO concentrations increased (p < 0.001). LYM apoptosis was comparable between techniques, but MONO apoptosis was higher after DGC than RBCL (p < 0.001).

Conclusions
Investigated MNC counts (LYM/MONO ratio) after cell isolation and staining did not always mimic WB conditions. Thus, final CPC results should be corrected accordingly, especially when reporting live CPC concentrations after DGC; otherwise, the CPC regenerative potential in circulation could be biased. This is of high importance in the context of non-invasively induced CPC mobilization such as by acute physical exercise, since these cell changes are small and conclusions drawn from published results might affect further applications of physical exercise as non-invasive therapy.
An efficient protocol to generate placental chorionic plate-derived mesenchymal stem cells with superior proliferative and immunomodulatory properties
Authors: Huang Qilin , Yang Yi , Luo Chen , Wen Yi , Liu Ruohong , Li Shuai , Chen Tao , Sun Hongyu , Tang Lijun 
Published: October 2019

Abstract

Background
Placenta-derived MSCs (P-MSCs) represent a promising tool for cell-based therapeutic applications. However, the increasing demand for P-MSCs in clinical trials makes high quality and large number of P-MSCs mandatory. Here, we aim to develop an efficient protocol for P-MSC isolation and culture.

Methods
The modified explant culture (MEC) method by combining an initial mild enzymatic reaction with the subsequent explant culture was developed to simultaneously produce various P-MSCs from the different regions of the placenta in serum-free medium (SFM). Its isolation efficiencies, cell yield, and proliferative capacity were compared with the conventional explant culture (EC) method. Furthermore, we determined whether functional properties of P-MSCs are affected by the used tissue-harvesting sites in terms of their proliferation, migration, and the immunomodulatory effect on macrophage.

Results
The MEC method achieved higher yield and shorter time in primary cell confluence in SFM compared with the conventional method. The harvested cells possessed the MSC characteristics and demonstrated significantly stronger proliferation ability. Importantly, MSCs derived from chorionic plate (CP-MSCs) were found to exhibit superior properties to the other P-MSCs in proliferation and migration capacity, maintaining the fetal origin over serial passages. Notably, CP-MSCs show stronger ability in regulating macrophage polarization from M1 to M2.

Conclusion
Our study developed an efficient and high-yield technique to produce high-quality P-MSCs from the placenta, hence serving as an optimal source of MSCs for clinical application.
New fractional inequalities of midpoint type via s-convexity and their application
Authors: Almutairi Ohud , Kılıçman Adem 
Published: October 2019

Abstract
In this study, we introduced new integral inequalities of Hermite–Hadamard type via s-convexity and studied their properties. The absolute form of the first and second derivatives for the new inequalities is considered to be s-convex. As an application, the inequalities were applied to the special means of real numbers. We give the error estimates for the midpoint formula.
Fractional Hermite–Hadamard type inequalities for interval-valued functions
Authors: Liu Xuelong , Ye Gouju , Zhao Dafang , Liu Wei 
Published: October 2019

Abstract
We introduce the concept of interval harmonically convex functions. By using two different classes of convexity, we get some further refinements for interval fractional Hermite–Hadamard type inequalities. Also, some examples are presented.
Ordinary and degenerate Euler numbers and polynomials
Authors: Kim Taekyun , Kim Dae San , Kim Han Young , Kwon Jongkyum 
Published: October 2019

Abstract
In this paper, we study some identities on Euler numbers and polynomials, and those on degenerate Euler numbers and polynomials which are derived from the fermionic p-adic integrals on $\mathbb{Z}_{p}$

Z
p

. Specifically, we obtain a recursive formula for alternating integer power sums and representations of alternating integer power sum polynomials in terms of Euler polynomials and Stirling numbers of the second kind, as well as various properties about Euler numbers and polynomials. In addition, we deduce representations of degenerate alternating integer power sum polynomials in terms of degenerate Euler polynomials and degenerate Stirling numbers of the second kind, as well as certain properties on degenerate Euler numbers and polynomials.
Existence and stability results for multi-time scale stochastic fractional neural networks
Authors: Wang Dehua , Ding Xiao-Li , Ahmad Bashir 
Published: October 2019

Abstract
We apply the tools of functional analysis to investigate the existence and uniqueness of solutions for multi-scale stochastic fractional neutral networks. By constructing a descent Lyapunov functional, the asymptotic stability of the solution of the given problem is also studied. Finally, we present two examples to illustrate the effectiveness of the theory.
First record of Burmeister’s porpoise (Phocoena spinipinnis) in the Falkland Islands (Malvinas)
Authors: Weir Caroline R. , Rutherford Susan 
Published: October 2019

Abstract
The Burmeister’s porpoise Phocoena spinipinnis is a small, poorly-studied, and Near Threatened cetacean species, with a limited geographic range comprising the Pacific and Atlantic coastal temperate waters of five South American countries. Here we describe a stranding of a freshly-dead adult male Burmeister’s porpoise that was discovered opportunistically on the eastern coast of the Falkland Islands (51°40.9′S, 57°43.2′W) on 29 June 2019. Unfortunately the carcass was removed by an unknown source prior to any scientific sampling, but the morphological features revealed in photographs taken of the dead animal were sufficient to verify the species identification. This is the first known record of Burmeister’s porpoise in the Falklands, confirming a sixth range state for the species and expanding its known distribution range eastwards from the previous nearest documented occurrence in Tierra del Fuego by at least 600 km across the Patagonian Shelf.