bluuebunny/crossref_metadata_2025_split huggingface dataset:
Study of the Association of V640L (rs6133) Polymorphism in the Platelet P-selectin Gene with Acetylsalicylic Acid Resistance in Patients after Coronary Bypass Surgery
Authors: Kosinova A. A. , Mongush T. S. , Goncharov M. D. , Subbotina T. N. , Semashchenko K. S. , Kochmareva G. Y. , Grinshtein Yu. I. 
Published: July 2019

Aim.To study the association of V640L (rs6133) polymorphism in the P-selectin gene with acetylsalicylic acid (ASA) resistance in patients with coronary heart disease after coronary bypass surgery (CABG).Material and methods.The study included 104 patients aged 36-78 years (mean age 61.6±6.9 years) with stable angina pectoris: 61 (58.7%) patients had functional class II (according to Canadian Cardiovascular Society), 41 (39.4%) – class III and 2 (1.9%) – class IV. Atherosclerotic lesions of the coronary arteries were confirmed by coronary angiography. The antiplatelet therapy was stopped for at least 5 days before CABG. In the postoperative period, from the first day, all patients received 100 mg of ASA in enteric form, 61 patients received alone ASA therapy, 43 patients – combined antiplatelet therapy: ASA+clopidogrel (75 mg/day). The aggregation study was performed with an optical aggregometer, using 5 μM adenosinediphosphate (ADP) and 1 mM arachidonic acid inductors before CABG, on 1-3 day and on 8-10 day after surgical treatment. DNA samples were examined for the V640L (rs6133) polymorphism in the P-selectin gene by real-time polymerase chain reaction (PCR) using the allele-specific primers.Results. The frequency of the homozygous GG genotype of the rs6133 polymorphism was 84.6%; heterozygous GT genotype – 15.4%. The amplitude of aggregation with ADP before CABG, on 1-3 day and on 8-10 day after CABG for carriers of homozygotes of allele G vs carriers of the allele T were: 47.9±19.3%, 44.5±17.8%, 30.1±13.2% vs 47.9±17.1%, 46.3±16.5%, 39.6±22.0%, respectively (p=0.497, 0.441 and 0.687, respectively). The amplitude of aggregation with arachidonic acid before CABG, on 1-3 day and on 8-10 day after CABG for carriers of homozygotesof allele G vs carriers of the allele T, were: 47.9±23.2%, 24.5±21.7%, 12.3±16.3% vs 54.3±17.8%, 29.7±23.7%, 11±10.9%, respectively (p=0.416, 0.825 and 0.872, respectively). In the first 10 days of the postoperative period, 6 thrombotic events (5.7%) were observed in the study group: 2 strokes and 4 perioperative myocardial infarctions. Five events occurred in the group of patients with the GG genotype, 1 event in the group of patients with the GT genotype.Conclusion. V640L (rs6133) polymorphism in the P-selectin gene is not associated with ASA resistance in patients with coronary artery disease after CABG. The T allele of the rs6133 polymorphism is not associated with increased platelet aggregation activity after CABG and does not increase the risk of adverse events in the first 10 days after CABG.
Multi-Ethnic Analysis of Cardiac Pharmacogenetic Markers of Cytochrome P450 and Membrane Transporters Genes in the Russian Population
Authors: Mirzaev K. B. , Fedorinov D. S. , Ivashchenko D. V. , Sychev D. A. 
Published: July 2019

Aim. To summarize Russian studies using pharmacogenetic testing as applied to cardiology.Material and methods. The authors conducted an online search for articles in December 2018 using the following databases: PubMed, Google Scholar, eLIBRARY. The search was carried out by keywords: "Russia", "Russian", "cardiology" together with the terms associated with the polymorphic marker, including: «P450», «CYP2C19», «CYP2D6», «CYP2B1», «CYP2B6», «CYP2Е1», «CYP2C8», «CYP2C9», «CYP3A4», «CYP3A5», «CYP1A1», «CYP1A2», «CYP4F2», «CYP4F1», «ABCB1», «SLCO1B1», «VKORC1», «GGCX», «SULT1A1», «CULT1», «CES1», «gene», «genes», «pharmacogenetics», «pharmacogenomics», «ethnic group».Results. Generalization of information allowed to identify obscure genes that need to be investigated in pharmacogenetic studies. This information can be used for the development of dosing algorithms and the priority choice of drugs, considering the results of pharmacogenetic testing and planning future research.Conclusion. The results of the literature review indicate the importance of studying the most clinically valid and clinically useful pharmacogenetic markers (CYP2C19, CYP2C9, VKORC1, SLCO1B1) among various ethnic groups in the Russian Federation. With the accumulation of evidence of clinical validity and clinical utility of other pharmacogenetic markers (CES1, CYP2D6*4, etc.), the problem of interethnic differences in the carriage of clinically significant polymorphisms of these genes identified in previous studies in the Russian Federation increasingly requires attention. The most promising for the introduction into the clinical practice in the Russian Federation in the near future are polymorphic markers of the CYP2C19, CYP2C9, VKORC1 and SLCO1B1 genes.
Analisis Tingkat Kesiapan Pemerintah Daerah dalam Pengambilalihan Pajak Bumi dan Bangunan Pedesaan dan Perkotaan 1 (PBB-P2) (Studi Kasus Pada Pemerintah Kota Pekanbaru)
Authors: Wulandari Hesty 
Published: July 2019

<p>This research is intended to know the level of preparedness of Local Government of Pekanbaru City in taking over the authority of management of Property Tax in Urban and Rural Area from central government. Readiness indicators are measured by local regulations, human resources, equipment, financing and cooperation with others. This research uses qualitative method where all data collected by observation and interview with official in Dispenda Office Pekanbaru City. The results showed that the Pekanbaru city government has been quite ready to take over the authority. It shows that almost all the needs that exist and two unmet needs are in the process.</p><p> </p><p>Penelitian ini dimaksudkan untuk mengetahui tingkat kesiapan Pemerintah Daerah Kota Pekanbaru dalam pengambilalihan kewenangan pengelolaan Pajak Bumi dan Bangunan Pedesaan dan Perkotaan dari pemerintah pusat. Indikator kesiapannya diukur dari peraturan daerah, sumber daya manusia, peralatan, pendanaan serta kerjasama dengan pihak lain. Penelitian ini menggunakan metode kualitatif dimana semua data dikumpulkan dengan observasi dan wawancara dengan pejabat berwenang di Kantor Dispenda Kota Pekanbaru. Hasil penelitian menunjukkan bahwa Pemda Kota Pekanbaru telah cukup siap untuk mengambil alih kewenangan ini terbukti dengan hampir terpenuhinya semua kebutuhan yang ada dan dua kebut uhan yang belum terpenuhi sedang berada dalam proses pengerjaan.</p>
Pola Pemanfataan Sumberdaya Laut Berbasis Kearifan Lokal Pada Masyarakat Wabula, di Kabupaten Buton
Authors: Mustari Tamar , Manaf Sulhan , Munafi La Ode Abdul 
Published: July 2019

<em>The research describes the patten of utilization of marine resources based on local wisdom in the Wabula community in Buton district. The data were collected by doing interwiews, observation, documentation, and study of literature. Then the data were analyzed descriptively and kualitatively. The results showed that in utilizing marine resources, Wabula community have a local wisdom called kaombo which is protected marine areas by the custom. When a marine area is designated as a kaombo a ban om the explotation of marine resources in the area applies. The prohibition is intended to provide a respite for the continuation of the proliferation of marine biota in kaombo ecosystem. A violation of the prohibition is subject to strict customary law. To preserve the local wisdom of Wabula community in the utilization of marine resources, then through the regulation of Buton Regent Number 13 of 2018, Wabula was designated as an area Customary Law Community in the management of marine resources based om local customary law. The regulation is considered as an ideal model for strengthening local community institutions in conserving marine resources in a sustainable manner.</em>
An Experimental Approach To Assess the Dispersion of Shale in Fracturing Fluids
Authors: Muggli I. de Araujo , Chellappah K. , Collins I. R. 
Published: July 2019

Summary
We propose an experimental approach to evaluate how typical fluids influence shale dispersion. In this approach, finely ground shale is left to settle in the fracturing fluid, generating particle-size and concentration profiles within the settling column. Samples are taken at various settling times and depths and then analyzed with regard to turbidity and capillary-suction-time (CST) behavior. Particle-size-distribution (PSD) measurements are used to further substantiate analysis. Turbidity data indicate the volume of particles present, and PSD data indicate the sizes of these particles (or flocs). This approach was tested using ground shale, Eagle Ford brine (EFB), and three typical fluid additives. Without additives present, shale flocculation resulted in rapid particle settling, and samples taken from suspension gave low turbidity and CST values. With additives present, suspensions were better dispersed and hence tended to give higher CST values. Some additives hindered flocculation more effectively than others. The results suggest that low CST numbers might not always be desirable; additives that are good inhibitors might hinder flocculation of shale particulates and hence promote higher CST numbers. In this paper we discuss how our proposed experimental approach can give insights into the influence of additives on the degree and nature of shale dispersion.
The Effects of Smoking-Cessation Counseling Based on the Stages-of-Change Model in Dental Patients; Semi-Experimental Study
Authors: Ghorbani Zahra , Ahmady Arezoo Ebn , hosseini zahra , Azimi Somayyeh 
Published: July 2019

Abstract
Background

Dentists may take part in smoking cessation counseling of dental patients by using the time they are engaged in dental procedures and by emphasizing on oral manifestations of smoking. The present study aimed to evaluate the effects of smoking cessation counseling by a dentist on preparation for change to quit smoking in smoker patients.

Methods

This study was performed on 150 smoking patients admitted to Dental School of Shahid Beheshti University of Medical sciences, Tehran, Iran. The patients were randomly divided into two groups of 75 patients. The intervention group received smoking cessation counseling by a single senior dental student. A self-administered questionnaire containing questions regarding the position of the individual in change cycle stages was completed by both intervention and control group at baseline and at one-month follow up. For statistical analysis, paired t-tests, Mann-Whitney U and multivariate linear regression models was used with a significance level of P<0.05.

Results

At baseline, there were no significant differences between the intervention and control groups in terms of change cycle stages of smoking cessation. Also, there was no significant difference between change cycle stages at baseline and one-month follow up in control group; but this difference in intervention group was significant (p=0.006). The proportion of patients in the pre-contemplation stage decreased by 43% while the proportion of patients in the contemplation and action stages increased by 20% and 16% respectively.

Conclusions

The smoking cessation counseling enhances forward movement through the stages-of-change of smoking cessation. Measurement of this movement may be an important intermediary in evaluating small clinical trials of counseling.
Prevalence and Associated Factors of Pneumonia among Children 2 – 59 Months of Age in Gumay District, Jimma Zone, Southwest Ethiopia
Authors: Tesfaye Anteneh , Hiko Desta , Kabeta Teshome 
Published: July 2019

Abstract
Background

Recent estimates from the World Health Organization suggest that pneumonia is responsible for 20% of deaths in the under-five age group, leading to 3 million deaths per year. Out of fifteen countries that have the highest death rate from clinical pneumonia in children younger than five-year-old, Ethiopia ranks as number four in the world.

Objective

To determine the prevalence and identify the associated factors of pneumonia among 2 -59 months old children in Gumay district, Jimma zone, 2017.

Methods

Community based cross sectional study was conducted in Gumay district from March 1- 26 /2017. Multi-stage sampling technique was used to proportionally draw 347 households from 5 selected kebeles. Pre-tested Interviewer administered structured questionnaire was employed to collect data from households. Health professionals were recruited in the survey as data collectors and supervisors. The data was entered to Epi-Data version 3 and then exported to SPSS version 20 for analysis.

Result

The prevalence of pneumonia in 2 to 59 months old children found to be 7.5%. a child 2-11 months of age(AOR = 3.17;95%CI 1.6,6.3; p-value= 0.024), Cooking place (AOR=5.7; 95% CI 1.83,18; p-value=0.004), living in houses with less than two windows (AOR= 3.18; 95% CI 1.07, 9.5; p-value =0.034), location of the child during cooking (AOR=5.15; 95%CI 1.6, 16.7; p-value= 0.008) and being not vaccinated (AOR=4.76, 95% CI;1.69, 13.37; p-value=0.003) were found to be significant associated with pneumonia among children 2 to 59 months of age in this study.
Integrated analysis of metabolomes and transcriptome between the testis of non-repropductive season and reproductive season of oriental river prawn, Macrobrachium nipponense
Authors: Jin Shubo 
Published: July 2019

Abstract
Background: The better understanding of male sexual differentiation and development mechanism of M. nipponense is urgently needed, in order to maintain the sustainable development of M. nipponense industry. In the present study, we aimed to identify the key genes and metabolites involved in the male sexual differentiation and development of M. nipponense through performing the integrated analysis of metabolomes and transcriptomes of testis between the reproductive season and non-reproductive season.
Results: A total of 385 differentially expressed metabolites (DEMs) and 12,230 differentially expressed genes (DEGs) were identified. Glycerophospholipid metabolism and Sphingolipid metabolism were predicted to have dramatic effects on male sexual differentiation and development in M. nipponense, based on the integrated analysis of metabolomes and transcriptomes. According to the KEGG enrichment analysis of DEGs, Oxidative phosphorylation, Glycolysis/Gluconeogenesis, HIF-1 signaling pathway, Citrate cycle, Steroid hormone synthesis and Spliceosome were predicted to promote the male differentiation and development through providing ATP, promoting the synthesis of steroid hormone and providing correct gene products. SDHB, PDE1, HSDL1, Cp450, SRSF and SNRNP40 from these metabolic pathways were predicted to have dramatic effects in M. nipponene through performing the qPCR analysis in different reproductive season of testis and various tissues.
Conclusion: This study provides new evidences for analyzing the process of male sexual differentiation and development in M. nipoponense.
Comparative genomics uncovers genetic diversity and synthetic biology of secondary metabolite production of the genus Trametes
Authors: Zhang Yan , Cao Jie , Wang Jingjing , Cheng Yajun , Zhou Minghui , Wang Wei , Geng Ming , Xu Decong , Xu Zhongdong 
Published: July 2019

Abstract
It’s well-established that the CAZyme genes of genus Trametes contributed to the degradation processes of polysaccharides, including lignin or crystalline cellulose. However, the comprehensive analysis of the composition of CAZymes and the biosynthetic gene clusters of Trametes genus remain unclear. We conducted comparative analysis, detected the CAZyme genes, and predicted the biosynthetic gene clusters for 9 Trametes strains. Among 82,053 homologous clusters we obtained for genus Trametes, we identified 8,518 core genes, 60,441 accessory genes and 13,094 specific genes. Our results showed that a large proportion of CAZyme genes were catalogued into glycoside hydrolases, glycosyltransferases, and carbohydrate esterases. The predicted BGCs of Trametes genus were divided into 6 strategies and the 9 Trametes strains harbored 47.78 BGCs on average. Our study uncovers the genus Trametes exhibited an open pan-genome structure, provides insights into the genetic diversity and explores the synthetic biology of secondary metabolite production for Trametes genus.
Multiple Iterative Labeling by Antibody Neodeposition (MILAN)
Authors: Cattoretti Giorgio , Bosisio Francesca Maria , Marcelis Lukas , Bolognesi Maddalena Maria 
Published: July 2019

Abstract

Multiplexing, labeling for multiple immunostains the very same cell or tissue section in situ, is of considerable interest. The major obstacles to the diffusion of this technique are high costs in custom antibodies and instruments, low throughput, scarcity of specialized skills or facilities.

We have validated and detail here a method based on common primary and secondary antibodies, diffusely available fluorescent image scanners and routinely processed tissue sections \(FFPE). It entails rounds of four-color indirect immunofluorescence, image acquisition and removal \(stripping) of the antibodies, before another stain is applied. The images are digitally registered and the autofluorescence is subtracted. Removal of antibodies is accomplished by disulphide cleavage. In excess of 50 different antibody stains can be applied to one single section from routinely fixed and embedded tissue. This method requires a modest investment in hardware and materials and uses freeware image analysis software.
Toward Overcoming Physical Disability in Spinal Cord Injury: A Qualitative inquiry of the Experiences of Injured Individuals and Their Families
Authors: Nikbakht-Nasrabadi Alireza , mmohammadi nooredin , Yazdanshenas Manijeh , Shabany Maryam 
Published: July 2019

Abstract
OBJECTIVES

Spinal cord injury (SCI) is a life-changing experience for the individuals with SCI and their families. This study aimed to investigate physical strategies used for overcoming physical disability in individuals with SCI.

METHODS

In this qualitative study, 17 SCI persons and 13 family caregivers were selected by a purposeful sampling. Settings of the study were Brain and SCI research (BASIR) center of Tehran University of Medical Sciences and Southern Social Welfare Center of Tehran and SCI Association of Tehran, Iran. Data were collected by face-to-face semi-structured interviews, which were continued until data saturation. The gathered data were concurrently analyzed by the content analysis method.

RESULTS

The data analysis revealed one main theme (towards overcoming physical disability) and three sub-themes: 1) physical rehabilitation by various methods; 2) tendency towards the use of alternative medical methods; and 3) making effort for self-reliance.

CONCLUSION

The participants used physiotherapy and occupational therapy as an effective and essential approach offered by the healthcare team. Some individuals with SCI with help of their family had invented simple rehabilitation equipment for help to their physical rehabilitation. However, most participants had referred to different complimentary medicine specialists based on advice friends and relatives and they often had spent a lot of time and money ineffectively. Therefore, they need training and support of the healthcare team as well as social support to achieve physical independence and physical recovery. Further research is suggested to investigate the barriers to achieving physical empowerment in people with SCI in Iran.
Pharmacokinetics of Recombinant Human Growth Hormone (rhGH) in Beagles by ELISA
Authors: Qian Yueyue , Shao Qing , Xu Quanyu , Qiao Hongqun 
Published: July 2019

Background::
Somatropin is recombinant human growth (GH) used for the treatment of
growth failure in children and GH deficiency in adults. At present, rhGH marketed in China is mostly
freeze-dried powder injection. As the lyophilization process is unstable, time-consuming and costly,
rhGH has been prepared into an aqueous solution for administering directly.

Introduction::
In this study, the pharmacokinetics of two dosage forms of rhGH in beagle dogs after
single subcutaneous administration was determined by enzyme-linked immunosorbent assay (ELISA).

Methods:
Twelve healthy beagles (male, 6:female, 6) were used for the pharmacokinetic study and
were equally divided into two groups. Subcutaneous injection of 0.2 IU/kg with rhGH in the two formulations.
The blood samples were taken from forearms, 0, 0.033, 0.083, 0.25, 0.5, 1, 2, 3, 4, 7, 10, 24 h
and collected the beagle plasma on time. The pharmacokinetic parameters of rhGH after subcutaneous
(s.c.) injection were determined experimentally on beagles. Primary PK endpoints were area under the
serum concentration-time curve (AUC0-t) and maximum serum concentration (Cmax). Serum rhGH level
was determined by enzyme-linked immunosorbent assay.

Results::
The calibration curves obtained were linear over the concentration range of 25 to 1600 ng/ml
for recombinant human growth. The results of the intra- and inter-day precision and accuracy studies
were well within the acceptable limits. The analysis samples were stable under different storage conditions
and temperature.

Conclusions::
The developed ELISA method has been successfully applied to the studies of pharmacokinetic
of recombinant human growth hormone in beagles.
Impacts of Wind Energy Integration to the Utility Grid and Grid Codes: A Review
Authors: Srivastava Puneet K. , Tiwari Amar N. , Singh Sri N. 
Published: July 2019

Background:
This paper reviews the requirements imposed by Transmission System
Operators (TSOs) in their grid codes to address intolerable implications of integrating large Wind
Power Plants (WPPs) to the grid. Besides the common objectives, terminologies used by different
TSOs were distinct. Therefore, developing a harmonized grid code is imperative.

Introduction:
On the recommendation of the European Commission, European Network of Transmission
System Operators for Electricity (ENTSO-E) has made concerted efforts in the direction of
developing a harmonized grid code, and published its document in March 2013.

Method:
Several TSOs have updated their grid codes in accordance with the increasing wind energy
penetration. We have made an effort to present a comparison between different integration requirements
specified by different grid codes. The outcomes presented will certainly be beneficial to
the countries, which are in the process of enacting their own grid code.

Result:
We have reviewed different grid codes in terms of Low Voltage Ride-Through (LVRT)
requirement, operating voltage and frequency range, active power control (frequency regulation)
and reactive power support (voltage regulation).

Conclusion:
The present study envisages helping wind turbine manufacturers and WPP developers,
as it would provide them a clear insight into the regulations imposed on WPPs as included/
updated in different grid codes.