bluuebunny/crossref_metadata_2025_split huggingface dataset:
Platelets from donors who use non‐steroidal anti‐inflammatory drugs are functional when stored under blood bank conditions
Authors: Bontekoe Ido J. , Groot Stéphanie A. , van der Meer Pieter F. , de Korte Dirk 
Published: November 2018

Background and ObjectivesIn our country, buffy coats (BCs) from donors who used non‐steroidal anti‐inflammatory drugs (NSAIDs) are not used for platelet (PLT) production, because NSAIDs inhibit platelet aggregation. We aimed to investigate the in vitro quality of PLT concentrates (PCs) prepared from NSAID BCs. Also a dose‐response study was performed with ibuprofen in PCs prepared from regular BCs.Materials and MethodsSingle‐donor PCs in plasma (sPCs, n = 18) were prepared from NSAID BCs. The type of NSAID was extracted from the donor questionnaire. Aliquots of pooled PC in PAS‐E (n = 3) were used to test dose‐response effects of ibuprofen. The PC (aliquots) was stored for 8 days at 22 ± 2°C and sampled regularly.ResultsMost NSAID donors used aspirin, diclofenac, ibuprofen or naproxen. Storage properties of PC were not measurably affected by NSAIDs. Aggregation tests with arachidonic acid revealed no differences with ibuprofen, which was in line with levels in donor plasma and the dose‐response study. A low or absent response was observed for sPC with aspirin (0, 0–60, median, range, P < 0·05), diclofenac (30, 0–93) and naproxen (0, 0–47, P < 0·05), compared to normal controls (69, 0–90). No differences were detected in aggregation with ADP or collagen.ConclusionNo effects of NSAIDs on platelet storage properties were measured, but some effects on aggregation properties were present. The use of BC with ibuprofen can be considered feasible for pooled BC, due to pooling and dilution with PAS. For the other NSAIDs, except for aspirin which inhibits PLTs irreversibly, further study of NSAID BCs in pools is recommended.
Nonarticular osseous cyst‐like lesions of the intermedioradial carpal bone may be incidental magnetic resonance imaging findings in dogs
Authors: Porter Ian R. , Pownder Sarah L. , McDonough Sean P. , Potter Hollis G. , Hayashi Kei 
Published: November 2018

AbstractAs magnetic resonance imaging (MRI) becomes more readily available and more frequently utilized in the assessment of canine carpal lameness, both normal variations and early pathologic conditions must be recognized to optimize patient care and provide accurate diagnosis. On cross sectional studies of the canine carpus, cyst‐like lesions have been detected at the dorsolateral aspect of the intermedioradial carpal bone. The cross‐sectional imaging and histologic properties of these lesions have not been described. The purpose of this observational study is to evaluate the MRI and histologic features of these cyst‐like lesions in a cohort of clinically sound dogs. It was hypothesized that the lesions would show features similar to intraosseous ganglion cysts of the human wrist. Twenty‐five cadaveric canine carpi were obtained and a total of 13 lesions were detected on MRI. Based on MRI, six carpi with lesions of varying size and one normal carpus were submitted for histological evaluation. Five of the abnormal carpi had nonarticular cyst‐like lesions; one specimen with a positive magnetic resonance image for a cyst‐like lesion had no cyst‐like lesion on histology. Conspicuity of a medium‐size lesion as evaluated on radiographs was poor. Given the presence of these nonarticular cyst‐like lesions in a population of clinically sound patients, their clinical importance is uncertain. The development of these lesions may relate to altered mechanics or genetic predispositions, requiring additional study.
Thoracic processi spinosi findings agree among subjective, semiquantitative, and modified semiquantitative scintigraphic image evaluation methods and partially agree with clinical findings in horses with and without thoracolumbar pain
Authors: van Zadelhoff Claudia , Ehrle Anna , Merle Roswitha , Jahn Werner , Lischer Christoph 
Published: November 2018

AbstractImpinging processi spinosi in the equine thoracic spine are a common cause of poor performance in the horse. A modified semiquantitative scintigraphic image analysis has been proposed for the evaluation of equine processi spinosi. This technique showed a high inter‐ and intraobserver agreement when compared to subjective and semiquantitative image analysis. The aim of this retrospective, method comparison study was to evaluate the agreement of the modified semiquantitative scintigraphic image assessment with previous methods of interpretation and to compare these scintigraphic evaluation techniques with radiographic and clinical findings. Two hundred twenty‐three Warmblood horses that underwent scintigraphic, radiographic, and clinical examination of the thoracic spine were included in the study. Scintigraphic images were assessed using subjective, semiquantitative, and modified semiquantitative techniques. Radiographs were subjectively graded and horses were assigned to a group with or without thoracolumbar pain. Total radiographic and total scintigraphic grades were higher in horses with thoracolumbar pain (P < 0.05). Both the semiquantitative and the modified semiquantitative uptake ratios did not differ significantly in horses with or without thoracolumbar pain. The kappa agreement showed a substantial agreement between the modified semiquantitative scintigraphic and the semiquantitative scintigraphic evaluation techniques. The agreement between subjective scintigraphic and modified semiquantitative scintigraphic image evaluations was fair. There was a slight agreement between all scintigraphic techniques and radiographic findings. Limitations were the definition of thoracolumbar pain and the image analysis being restricted to the caudal thoracic processi spinosi. In conclusion, the modified semiquantitative scintigraphic image assessment obtained consistent results but did not perform better than previous evaluation methods. Further comparison to a defined diagnosis is warranted.
Point-of-care measurements of blood ketones in newborns
Authors: Harris Deborah L , Weston Philip J , Harding Jane E 
Published: November 2018

ObjectiveBabies may use alternative cerebral fuels including ketones when blood glucose concentrations are low, but laboratory ketone measurements are slow and expensive. Point-of-care measurement of ketone concentrations, if sufficiently accurate, may provide useful information for clinical care.Patients and designEligible babies were 35–42 weeks’ gestation, ≤10 days old and admitted to the newborn intensive care unit. At the time of clinically indicated blood tests, additional samples were taken to measure beta-hydroxybutyrate using a point-of-care analyser and the laboratory method.ResultsOne-hundred and fifty babies had 142 paired samples. Overall point-of-care accuracy was excellent (mean difference 0.00 mmol/L) and precision was moderate (SD 0.18 mmol/L). A point-of-care measurement ≥0.4 mmol/L was highly predictive of a laboratory measurement ≥0.4 mmol/L (area under the curve 0.98).ConclusionPoint-of-care measurement of blood beta-hydroxybutyrate concentrations is sufficiently accurate in newborns to be potentially useful in clinical care.Clinical trial registration numberRegistered with the Australian and New Zealand Clinical Trials Registry ACTRN: 12616000784415. The study was registered before recruitment commenced.
Comparison of efficacy and safety between electroacupuncture at ‘four sacral points’ and conventional electroacupuncture for the treatment of urinary incontinence after stroke: study protocol for a randomised controlled trial
Authors: Chen Shan , Wang Siyou , Xuan Lihua , Lu Hanti , Hu Zhikai , Zhang Chao , Zhang Huifang 
Published: November 2018

IntroductionElectroacupuncture at ‘four sacral points’, also known as electrical pudendal nerve stimulation therapy, combines the advantages of pudendal nerve neuromodulation and the technique of deep insertion of long acupuncture needles. It has been used to treat stress urinary incontinence, female urgency-frequency syndrome, idiopathic urgency urinary incontinence and neurological bladders in previous studies. Here, we describe the protocol for a randomised controlled trial for evaluation of the efficacy and safety of electroacupuncture at ‘four sacral points’ for the management of urinary incontinence after stroke.Methods and analysisThis is an open-label randomised controlled trial with blinded assessments and analyses. A total of 140 eligible patients will be randomly allocated to two groups. The treatment group (n=70) will receive electroacupuncture at ‘four sacral points’ along with routine medical care, while the control group will receive conventional electroacupuncture along with routine medical care. Twenty treatment sessions will occur over a period of 4 weeks. The primary outcome measures will be the self-recorded findings in an incontinent episode diary at baseline and at 4 weeks after baseline. The secondary outcome measures will be the International Consultation on Incontinence Questionnaire Urinary Incontinence—Short Form (ICIQ-UI SF) score and the Barthel Activities of Daily Living Index (Barthel ADL Index) score at baseline and at 4 and 28 weeks after baseline.Ethics and disseminationThis protocol has been approved by the Ethics Committee of the First Affiliated Hospital of Zhejiang Chinese Medical University (approval No. 2018-K-059–01). Written informed consent will be obtained from each participant. The results of the study will be published in peer-reviewed journals.Trial registration numberChiCTR-IOR-17012847; Pre-result.
Nocturnal respiratory rate as a predictor of mortality in patients with acute coronary syndrome
Authors: Eick Christian , Groga-Bada Patrick , Reinhardt Kathrin , Duckheim Martin , Mizera Lars , Böhm Katharina , Götz Nina , Gawaz Meinrad , Zürn Christine 
Published: November 2018

BackgroundPatients with acute coronary syndrome (ACS) are at risk especially in the period shortly after the event. Alterations in respiratory control have been associated with adverse prognosis. The aim of our study was to assess if the nocturnal respiratory rate (NRR) is a predictor of mortality in patients with ACS presenting in the emergency department.MethodsClinically stable consecutive patients with ACS aged ≥ 18 years were prospectively enrolled. The Global Registry of Acute Coronary Events (GRACE) score and left ventricular ejection fraction (LVEF) were assessed for all patients. The average NRR over a period of 6  hours was determined by the records of the surveillance monitors in the first night after admission. Primary and secondary endpoints were intrahospital and 2  years all-cause mortality, respectively.ResultsOf the 860 patients with ACS, 21 (2.4%) died within the intrahospital phase and 108 patients (12.6%) died within the subsequent 2 years. The NRR was a significant predictor of both endpoints and was independent from the GRACE score and LVEF. Implementing the NRR into the GRACE risk model leads to a significant increase of the C-statistics especially for prediction of intrahospital mortality.ConclusionThe NRR is an independent predictor of mortality in patients with ACS.
The physical properties of ThCr2Si2-type superconductors MPd2P2 (M = Y, La): A theoretical investigation
Authors: Lokman Ali Md. , Tosaddek Hossen Md. , Alam Shahin , Sadek Hossain Md. , Islam Jakiul , Rubayatul Islam SM. 
Published: November 2018

The structural stability, elastic, mechanical, optical characteristics and Debye temperature of single crystalline superconductors MPd2P2 (M = Y, La) were investigated by using the ab initio technique. We have carried out the plane wave pseudopotential within the generalized gradient approximation (GGA) implemented in the CASTEP computer code. Our investigated results of structural data are in well consistent with the previous experimental data. The bulk modulus B, shear modulus G, Young’s modulus E, Poisson’s ratio v, hardness H, and anisotropic factor A of MPd2P2 (M = Y, La) compounds were evaluated from the calculated elastic constants. The analysis of ratio B/G shows that the MPd2P2 superconductors are in ductile behavior. The Debye temperatures are also investigated from the elastic constants. Finally, the optical functions including reflectivity, absorption coefficient, loss function, conductivity, refractive index, dielectric function are calculated and analyzed.
A Combination CDK4/6 and IGF1R Inhibitor Strategy for Ewing Sarcoma
Authors: Guenther Lillian M. , Dharia Neekesh V. , Ross Linda , Conway Amy , Robichaud Amanda L. , Catlett Jerrel L. , Wechsler Caroline S. , Frank Elizabeth S. , Goodale Amy , Church Alanna J. , Tseng Yuen-Yi , Guha Rajarshi , McKnight Crystal G. , Janeway Katherine A. , Boehm Jesse S. , Mora Jaume , Davis Mindy I. , Alexe Gabriela , Piccioni Federica , Stegmaier Kimberly 
Published: November 2018

Abstract

Purpose:
Novel targeted therapeutics have transformed the care of subsets of patients with cancer. In pediatric malignancies, however, with simple tumor genomes and infrequent targetable mutations, there have been few new FDA-approved targeted drugs. The cyclin-dependent kinase (CDK)4/6 pathway recently emerged as a dependency in Ewing sarcoma. Given the heightened efficacy of this class with targeted drug combinations in other cancers, as well as the propensity of resistance to emerge with single agents, we aimed to identify genes mediating resistance to CDK4/6 inhibitors and biologically relevant combinations for use with CDK4/6 inhibitors in Ewing.

Experimental Design:
We performed a genome-scale open reading frame (ORF) screen in 2 Ewing cell lines sensitive to CDK4/6 inhibitors to identify genes conferring resistance. Concurrently, we established resistance to a CDK4/6 inhibitor in a Ewing cell line.

Results:
The ORF screen revealed IGF1R as a gene whose overexpression promoted drug escape. We also found elevated levels of phospho-IGF1R in our resistant Ewing cell line, supporting the relevance of IGF1R signaling to acquired resistance. In a small-molecule screen, an IGF1R inhibitor scored as synergistic with CDK4/6 inhibitor treatment. The combination of CDK4/6 inhibitors and IGF1R inhibitors was synergistic in vitro and active in mouse models. Mechanistically, this combination more profoundly repressed cell cycle and PI3K/mTOR signaling than either single drug perturbation.

Conclusions:
Taken together, these results suggest that IGF1R inhibitors activation is an escape mechanism to CDK4/6 inhibitors in Ewing sarcoma and that dual targeting of CDK4/6 inhibitors and IGF1R inhibitors provides a candidate synergistic combination for clinical application in this disease.
Targeting an Autocrine Regulatory Loop in Cancer Stem-like Cells Impairs the Progression and Chemotherapy Resistance of Bladder Cancer
Authors: Wang Kai-Jian , Wang Chao , Dai Li-He , Yang Jun , Huang Hai , Ma Xiao-Jing , Zhou Zhe , Yang Ze-Yu , Xu Wei-Dong , Hua Mei-Mian , Lu Xin , Zeng Shu-Xiong , Wang Hui-Qing , Zhang Zhen-Sheng , Cheng Yan-Qiong , Liu Dan , Tian Qin-Qin , Sun Ying-Hao , Xu Chuan-Liang 
Published: November 2018

AbstractPurpose:Cancer stem-like cells (CSCs) contribute to bladder cancer chemotherapy resistance and progression, but the associated mechanisms have not been elucidated. This study determined whether blocking an autocrine signaling loop in CSCs improves the therapeutic effects of cis-platinum on bladder cancer.Experimental Design:The expression of the epithelial marker OV6 and other markers in human bladder cancer specimens was examined by IHC. The CSC properties of magnetic-activated cell sorting (MACS)-isolated OV6+ and OV6− bladder cancer cells were examined. Molecular mechanisms were assessed through RNA-Seq, cytokine antibody arrays, co-immunoprecipitation (co-IP), chromatin immunoprecipitation (ChIP) and other assays. An orthotopic bladder cancer mouse model was established to evaluate the in vivo effects of a YAP inhibitor (verteporfin) and a PDGFR inhibitor (CP-673451) on the cis-platinum resistance of OV6+ CSCs in bladder cancer.Results:Upregulated OV6 expression positively associated with disease progression and poor prognosis for bladder cancer patients. Compared with OV6− cells, OV6+ bladder cancer cells exhibited strong CSC characteristics, including self-renewal, tumor initiation in NOD/SCID mice, and chemotherapy resistance. YAP, which maintains the stemness of OV6+ CSCs, triggered PDGFB transcription by recruiting TEAD1. Autocrine PDGF-BB signaling through its receptor PDGFR stabilized YAP and facilitated YAP nuclear translocation. Furthermore, blocking the YAP/TEAD1/PDGF-BB/PDGFR loop with verteporfin or CP-673451 inhibited the cis-platinum resistance of OV6+ bladder cancer CSCs in an orthotopic bladder cancer model.Conclusions:OV6 could be a helpful indicator of disease progression and prognosis for patients with bladder cancer, and targeting the autocrine YAP/TEAD1/PDGF-BB/PDGFR loop might serve as a remedy for cis-platinum resistance in patients with advanced bladder cancer.
A Combined Nomogram Model to Preoperatively Predict Histologic Grade in Pancreatic Neuroendocrine Tumors
Authors: Liang Wenjie , Yang Pengfei , Huang Rui , Xu Lei , Wang Jiawei , Liu Weihai , Zhang Lele , Wan Dalong , Huang Qiang , Lu Yao , Kuang Yu , Niu Tianye 
Published: November 2018

Abstract

Purpose:
The purpose of this study is to develop and validate a nomogram model combing radiomics features and clinical characteristics to preoperatively differentiate grade 1 and grade 2/3 tumors in patients with pancreatic neuroendocrine tumors (pNET).
Experimental Design: A total of 137 patients who underwent contrast-enhanced CT from two hospitals were included in this study. The patients from the second hospital (n = 51) were selected as an independent validation set. The arterial phase in contrast-enhanced CT was selected for radiomics feature extraction. The Mann–Whitney U test and least absolute shrinkage and selection operator regression were applied for feature selection and radiomics signature construction. A combined nomogram model was developed by incorporating the radiomics signature with clinical factors. The association between the nomogram model and the Ki-67 index and rate of nuclear mitosis were also investigated respectively. The utility of the proposed model was evaluated using the ROC, area under ROC curve (AUC), calibration curve, and decision curve analysis (DCA). The Kaplan–Meier (KM) analysis was used for survival analysis.

Results:
An eight-feature–combined radiomics signature was constructed as a tumor grade predictor. The nomogram model combining the radiomics signature with clinical stage showed the best performance (training set: AUC = 0.907; validation set: AUC = 0.891). The calibration curve and DCA demonstrated the clinical usefulness of the proposed nomogram. A significant correlation was observed between the developed nomogram and Ki-67 index and rate of nuclear mitosis, respectively. The KM analysis showed a significant difference between the survival of predicted grade 1 and grade 2/3 groups (P = 0.002).

Conclusions:
The combined nomogram model developed could be useful in differentiating grade 1 and grade 2/3 tumor in patients with pNETs.
ПОРІВНЯЛЬНЕ ВИПРОБУВАННЯ ОРГАННОЇ МОДЕЛІ ДЛЯ ДОСЛІДЖЕННЯ ЕЛЕКТРОЗВАРНОГО МІЖКИШКОВОГО АНАСТОМОЗУ В ЛАБОРАТОРНОМУ ЕКСПЕРИМЕНТІ
Authors: Podpriatov S. S. , Podpryatov S. Ye. , Gichka S. G. , Hetman V. G. , Makarov A. V. , Marinsky G. S. , Tkachenko V. A. , Chernets O. V. , Vasylchenko V. A. , Petrenko O. F. , Tarnavsky D. V. 
Published: November 2018

При заміщенні живої свині органною моделлю кишки людини зі свинячого органокомплексу для потреб експерименту незрозумілою є повноцінність збереження діелектричних властивостей стінки кишки та можливості утворення в ній субстрату електрозварного з’єднання.
Мета – визначити відповідність обраної органної моделі потребам лабораторного етапу відпрацювання умов створення міжкишкового анастомозу з застосуванням методу електрозварювання, замість проведення гострого експерименту на тварині.
Матеріал і методи. Дослідили особливості зміни товщини, імпедансу та утворення субстрату електрозварного з’єднання в тканинах тонкої кишки діаметром 24–27 мм та товстої кишки діаметром 27–31 мм. Органною моделлю слугував органокомплекс свині. Його охолоджували до 4 ºС та протягом 6–10 годин доставляли до лабораторії. Там біоімітатор занурювали у теплий (26–32 ºС) розчин 0,9 % NaCl на 10–20 хв. Отримані показники порівняли з отриманими в гострому експерименті на свині масою 45 кг, за згодою комітету з біоетики. Створили 8 електрозварних анастомозів на тваринній моделі та 52 на органокомплексі. Ззовні на електроди прикладали тиск 2,1 Н/мм2 або 3,0 Н/мм2. Подавали імпульсну високочастотну напругу, що рівномірно зростала від 80 В до 120 В впродовж 0,2 секунди. Ділянку з’єднання кишки видаляли для гістологічного дослідження.
Результати. Під зовнішнім стисненням ми відзначили подібність еластичності та щільності, на межі еластичності. У живої тварини була вища об’ємна резистентність м’язового шару, але динаміка стоншання – тотожною, що свідчить про подібність структурної міцності шарів тканини. У первинному імпульсі імпеданс плавно знижувався, після чого плавно зростав. У наступному імпульсі імпеданс миттєво падав, а потім майже лінійно зростав впродовж всього імпульсу. Подібна форма реактивності імпедансу стабілізувалася з другого імпульсу в 92,3 % проб на органокомплексі та 96,2 % – на тварині. В усіх дослідженнях утворювалось щільне з’єднання внаслідок коагуляційних змін пучків гладеньком’язових волокон та колагенових волокон неоднорідної глибини, але з утворенням суцільної безперервної структури.
Висновки. Динаміка стиснення, перебіг електрозварного імпульсу крізь тканину та структура електрозварного анастомозу при використанні органокомплексу були такими ж, як і під час гострого експерименту на тварині.
З огляду на синергійність впливу на тканини кишки при створенні електрозварного анастомозу, розробка технології його створення потребує проведення численних експериментальних досліджень. Враховуючи відтворення у дослідженій органній моделі базових механічних та електричних характеристик живої тканини та очікуваних морфологічних електрозварних перетворень можна зробити висновок, що існує можливість повноцінного заміщення тваринної моделі на цій стадії розробки та проведення тривалого лабораторного експерименту.