bluuebunny/crossref_metadata_2025_split huggingface dataset:
Is the Noble Gas‐Based Rate of Ocean Warming During the Younger Dryas Overestimated?
Authors: Shackleton S. , Bereiter B. , Baggenstos D. , Bauska T. K. , Brook E. J. , Marcott S. A. , Severinghaus J. P. 
Published: May 2019

AbstractNoble gases in ice cores enable reconstructions of past mean ocean temperature. A recent result from the clathrate‐containing WAIS Divide Ice Core showed tight covariation between ocean and Antarctic temperatures throughout the last deglaciation, except for the Younger Dryas interval. In the beginning of this interval, oceans warmed at 2.5 °C/kyr—three times greater than estimates of modern warming. If valid, this challenges our understanding of the mechanisms controlling ocean heat uptake. Here we reconstruct mean ocean temperature with clathrate‐free ice samples from Taylor Glacier to test these findings. The two records agree in net temperature change over the Younger Dryas, but the Taylor Glacier record suggests sustained warming at the more modest rate of 1.1 ± 0.2°C/kyr. We explore mechanisms to explain differences between records and suggest that the noble gas content for the Younger Dryas interval of WAIS Divide may have been altered by a decimeter‐scale fractionation during bubble‐clathrate transformation.
Geographical and Seasonal Variability of Mesospheric Bores Observed from the International Space Station
Authors: Hozumi Yuta , Saito Akinori , Sakanoi Takeshi , Yamazaki Atsushi , Hosokawa Keisuke , Nakamura Takuji 
Published: May 2019

AbstractGeographical and seasonal variations of mesospheric bores were derived from mesospheric airglow observations by the Visible and near Infrared Spectral Imager (VISI) of Ionosphere, Mesosphere, upper Atmosphere and Plasmasphere (IMAP) mission onboard the International Space Station. In the three‐year data set spanning September 2012 to August 2015, 306 mesospheric bore events were found between 55ºS and 55ºN in the O2(0‐0) airglow whose peak height is around 95 km. The distribution of the bore events showed a high occurrence at equatorial latitudes especially during the equinox seasons and at winter midlatitudes. These latitudes and seasons are also known for being the place and time where the migrating diurnal and semidiurnal tides have a large temperature amplitude at the upper mesosphere altitude. This coincidence suggests that the majority of mesospheric bores occurred in a temperature inversion layer, which is related to the tides. The local time variation of the bore occurrences at midlatitudes showed a minimum around midnight. The local time variation at equatorial latitudes is more widely distributed compared to those at midlatitudes. The dominant propagation direction of mesospheric bores is from the winter hemisphere to the summer hemisphere.
Caspase-1 initiates apoptosis in the absence of gasdermin D
Authors: Tsuchiya Kohsuke , Nakajima Shinsuke , Hosojima Shoko , Thi Nguyen Dinh , Hattori Tsuyoshi , Manh Le Thuong , Hori Osamu , Mahib Mamunur Rashid , Yamaguchi Yoshifumi , Miura Masayuki , Kinoshita Takeshi , Kushiyama Hiroko , Sakurai Mayumi , Shiroishi Toshihiko , Suda Takashi 
Published: May 2019

AbstractCaspase-1 activated in inflammasomes triggers a programmed necrosis called pyroptosis, which is mediated by gasdermin D (GSDMD). However, GSDMD-deficient cells are still susceptible to caspase-1-mediated cell death. Therefore, here, we investigate the mechanism of caspase-1-initiated cell death in GSDMD-deficient cells. Inflammasome stimuli induce apoptosis accompanied by caspase-3 activation in GSDMD-deficient macrophages, which largely relies on caspase-1. Chemical dimerization of caspase-1 induces pyroptosis in GSDMD-sufficient cells, but apoptosis in GSDMD-deficient cells. Caspase-1-induced apoptosis involves the Bid-caspase-9-caspase-3 axis, which can be followed by GSDME-dependent secondary necrosis/pyroptosis. However, Bid ablation does not completely abolish the cell death, suggesting the existence of an additional mechanism. Furthermore, cortical neurons and mast cells exhibit little or low GSDMD expression and undergo apoptosis after oxygen glucose deprivation and nigericin stimulation, respectively, in a caspase-1- and Bid-dependent manner. This study clarifies the molecular mechanism and biological roles of caspase-1-induced apoptosis in GSDMD-low/null cell types.
Tuning the charge flow between Marcus regimes in an organic thin-film device
Authors: Atxabal A. , Arnold T. , Parui S. , Hutsch S. , Zuccatti E. , Llopis R. , Cinchetti M. , Casanova F. , Ortmann F. , Hueso L. E. 
Published: May 2019

AbstractMarcus’s theory of electron transfer, initially formulated six decades ago for redox reactions in solution, is now of great importance for very diverse scientific communities. The molecular scale tunability of electronic properties renders organic semiconductor materials in principle an ideal platform to test this theory. However, the demonstration of charge transfer in different Marcus regions requires a precise control over the driving force acting on the charge carriers. Here, we make use of a three-terminal hot-electron molecular transistor, which lets us access unconventional transport regimes. Thanks to the control of the injection energy of hot carriers in the molecular thin film we induce an effective negative differential resistance state that is a direct consequence of the Marcus Inverted Region.
TFIIE orchestrates the recruitment of the TFIIH kinase module at promoter before release during transcription
Authors: Compe Emmanuel , Genes Carlos M. , Braun Cathy , Coin Frederic , Egly Jean-Marc 
Published: May 2019

AbstractIn eukaryotes, the general transcription factors TFIIE and TFIIH assemble at the transcription start site with RNA Polymerase II. However, the mechanism by which these transcription factors incorporate the preinitiation complex and coordinate their action during RNA polymerase II transcription remains elusive. Here we show that the TFIIEα and TFIIEβ subunits anchor the TFIIH kinase module (CAK) within the preinitiation complex. In addition, we show that while RNA polymerase II phosphorylation and DNA opening occur, CAK and TFIIEα are released from the promoter. This dissociation is impeded by either ATP-γS or CDK7 inhibitor THZ1, but still occurs when XPB activity is abrogated. Finally, we show that the Core-TFIIH and TFIIEβ are subsequently removed, while elongation factors such as DSIF are recruited. Remarkably, these early transcriptional events are affected by TFIIE and TFIIH mutations associated with the developmental disorder, trichothiodystrophy.
Towards a global DNA barcode reference library for quarantine identifications of lepidopteran stemborers, with an emphasis on sugarcane pests
Authors: Lee Timothy R. C. , Anderson Stacey J. , Tran-Nguyen Lucy T. T. , Sallam Nader , Le Ru Bruno P. , Conlong Desmond , Powell Kevin , Ward Andrew , Mitchell Andrew 
Published: May 2019

AbstractLepidopteran stemborers are among the most damaging agricultural pests worldwide, able to reduce crop yields by up to 40%. Sugarcane is the world’s most prolific crop, and several stemborer species from the families Noctuidae, Tortricidae, Crambidae and Pyralidae attack sugarcane. Australia is currently free of the most damaging stemborers, but biosecurity efforts are hampered by the difficulty in morphologically distinguishing stemborer species. Here we assess the utility of DNA barcoding in identifying stemborer pest species. We review the current state of the COI barcode sequence library for sugarcane stemborers, assembling a dataset of 1297 sequences from 64 species. Sequences were from specimens collected and identified in this study, downloaded from BOLD or requested from other authors. We performed species delimitation analyses to assess species diversity and the effectiveness of barcoding in this group. Seven species exhibited <0.03 K2P interspecific diversity, indicating that diagnostic barcoding will work well in most of the studied taxa. We identified 24 instances of identification errors in the online database, which has hampered unambiguous stemborer identification using barcodes. Instances of very high within-species diversity indicate that nuclear markers (e.g. 18S, 28S) and additional morphological data (genitalia dissection of all lineages) are needed to confirm species boundaries.
Genome-wide characterization of ALDH Superfamily in Brassica rapa and enhancement of stress tolerance in heterologous hosts by BrALDH7B2 expression
Authors: Gautam Ranjana , Ahmed Israr , Shukla Pawan , Meena Rajesh Kumar , Kirti P. B. 
Published: May 2019

AbstractAldehyde dehydrogenase (ALDH) carries out oxidation of toxic aldehydes using NAD+/NADP+ as cofactors. In the present study, we performed a genome-wide identification and expression analysis of genes in the ALDH gene family in Brassica rapa. A total of 23 ALDH genes in the superfamily have been identified according to the classification of ALDH Gene Nomenclature Committee (AGNC). They were distributed unevenly across all 10 chromosomes. All the 23 Brassica rapa ALDH (BrALDH) genes exhibited varied expression patterns during treatments with abiotic stress inducers and hormonal treatments. The relative expression profiles of ALDH genes in B. rapa showed that they are predominantly expressed in leaves and stem suggesting their function in the vegetative tissues. BrALDH7B2 showed a strong response to abiotic stress and hormonal treatments as compared to other ALDH genes; therefore, it was overexpressed in heterologous hosts, E. coli and yeast to study its possible function under abiotic stress conditions. Over-expression of BrALDH7B2 in heterologous systems, E. coli and yeast cells conferred significant tolerance to abiotic stress treatments. Results from this work demonstrate that BrALDH genes are a promising and untapped genetic resource for crop improvement and could be deployed further in the development of drought and salinity tolerance in B. rapa and other economically important crops.
Hemopoietic Cell Kinase amplification with Protein Tyrosine Phosphatase Receptor T depletion leads to polycythemia, aberrant marrow erythoid maturation, and splenomegaly
Authors: Ku Matthew , MacKinnon Ruth N. , Wall Meaghan , Narayan Nisha , Walkley Carl , Cheng Heung-Chin , Campbell Lynda J. , Purton Louise E. , Nandurkar Harshal 
Published: May 2019

AbstractDeletion of long arm of chromosome 20 [del(20q)] is the second most frequent recurrent chromosomal abnormality in hematological malignancies. It is detected in 10% of myeloproliferative neoplasms, 4–5% of myelodysplastic syndromes, and 1–2% of acute myeloid leukaemia. Recurrent, non-random occurrence of del(20q) indicates that it is a pathogenic driver in myeloid malignancies. Genetic mapping of patient samples has identified two regions of interest on 20q – the “Common Deleted Region” (CDR) and “Common Retained Region” (CRR), which was often amplified. We proposed that the CDR contained tumor suppressor gene(s) (TSG) and the CRR harbored oncogene(s); loss of a TSG together with over-expression of an oncogene favored development of myeloid malignancies. Protein Tyrosine Phosphatase Receptor T (PTPRT) and Hemopoietic cell kinase (HCK) were identified to be the likely candidate TSG and oncogene respectively. Retroviral transduction of HCK into PTPRT-null murine LKS+ stem and progenitor cells resulted in hyperproliferation in colony forming assays and hyperphosphorylation of intracellular STAT3. Furthermore, over half of the murine recipients of these transduced cells developed erythroid hyperplasia, polycythemia and splenomegaly at 12 months, although no leukemic phenotype was observed. The findings suggested that HCK amplification coupled with PTPRT loss in del(20q) leads to development of a myeloproliferative phenotype.
A Biobehavioral Validation of the Taylor Aggression Paradigm in Female Adolescents
Authors: Rinnewitz Lena , Parzer Peter , Koenig Julian , Bertsch Katja , Brunner Romuald , Resch Franz , Kaess Michael 
Published: May 2019

AbstractThis research assessed the behavioral, emotional, endocrinological and autonomic reactivity to the laboratory Taylor Aggression Paradigm (TAP) in a sample of healthy female adolescents. Twenty participants were induced with the TAP to behave aggressively (aggression group) and 20 age-matched participants were not induced to behave aggressively (control group). Regression analysis revealed that the aggression group displayed significant higher levels of aggressive behavior compared to the control group (χ2 (2) = 255.50, p < 0.0001). Aggressive behavior was not related to self-reported measures of trait aggression, impulsiveness or psychopathy features. Regarding the biological responses, regression analysis on cortisol, missed the set level of significance (χ2 (1) = 3.73, p = 0.054), but showed significant effects on heart rate as a function of aggression induction (χ2 (1) = 5.81, p = 0.016). While aggression induction was associated with increased autonomic arousal (heart rate), the interpretation of the effects on cortisol warrant caution, given existing differences between groups at baseline and overly elevated cortisol attributable to the general experimental procedures and not the TAP per se. No differences were found with respect to testosterone. In summary, the present study lends preliminary support for the validity of the TAP and its use in female adolescents on a behavioral and autonomic level.
Utilizing a Simple Method for Stoichiometric Protein Labeling to Quantify Antibody Blockade
Authors: Friedman Ohana Rachel , Hurst Robin , Rosenblatt Mike , Levin Sergiy , Machleidt Thomas , Kirkland Thomas A. , Encell Lance P. , Robers Matthew B. , Wood Keith V. 
Published: May 2019

AbstractLigand binding assays routinely employ fluorescently-labeled protein ligands to quantify the extent of binding. These ligands are commonly generated through chemical modification of accessible lysine residues, which often results in heterogeneous populations exhibiting variable binding properties. This could be remedied by quantitative, site-specific labeling. Recently, we reported on a single-step method integrating recombinant protein purification with 2-cyanobenzothiazole (CBT) condensation for labeling a proteolytically exposed N-terminal cysteine. Here, using three growth factors, we show that unlike random lysine labeling, this site-specific approach yielded homogeneous populations of growth factors that were quantitatively labeled at their N-termini and retained their binding characteristics. We demonstrate the utility of this labeling method through the development of a novel assay that quantifies the capacity of antibodies to block receptor-ligand interactions (i.e. antibody blockade). The assay uses bioluminescence resonance energy transfer (BRET) to detect binding of CBT-labeled growth factors to their cognate receptors genetically fused to NanoLuc luciferase. The ability of antibodies to block these interactions is quantified through decrease in BRET. Using several antibodies, we show that the assay provides reliable quantification of antibody blockade in a cellular context. As demonstrated here, this simple method for generating uniformly-labeled proteins has potential to promote more accurate and robust ligand binding assays.
Activities of starch synthetic enzymes and contents of endogenous hormones in waxy maize grains subjected to post-silking water deficit
Authors: Yang Huan , Gu Xiaotian , Ding Mengqiu , Lu Weiping , Lu Dalei 
Published: May 2019

AbstractRainfed maize in Southern China and frequently suffer water deficit at later plant growth periods. A pot trial in 2014–2015 was conducted to study the effects of drought stress (the relative soil moisture contents are 70–80% and 50–60% under control and water deficit conditions, respectively) after pollination on grain filling and starch accumulation, activities of starch synthetic enzymes, and contents of indole-3-acetic acid (IAA) and abscisic acid (ABA), with Suyunuo5 as test material. The grain fresh weight, volume, and dry weight were not affected by drought before 10 days after pollination but were restricted thereafter. The reduction at maturity was reduced by 33.3%, 40.0%, and 32.3% in 2014 and by 21.7%, 24.3%, and 18.3% in 2015. The grain filling rate was suppressed by water deficit, whereas grain moisture and starch content were slightly affected. The starch accumulation was decreased by 33.5% and 20.0% at maturity in 2014 and 2015, respectively. The activities of starch synthetic enzymes (sucrose phosphate synthase, sucrose synthase, ADP-glucose pyrophosphorylase, soluble starch synthase, and starch branching enzyme) were downregulated by post-silking drought. The ABA content was increased, whereas IAA content was decreased when plants suffered water deficit during grain filling. In conclusion, post-silking water deficit increased ABA content, decreased IAA content, and weakened the activities of starch synthetic enzymes, which suppressed grain development and ultimately reduced grain weight.
Chemosynthetic ectosymbionts associated with a shallow-water marine nematode
Authors: Bellec Laure , Bonavita Marie-Anne Cambon , Hourdez Stéphane , Jebbar Mohamed , Tasiemski Aurélie , Durand Lucile , Gayet Nicolas , Zeppilli Daniela 
Published: May 2019

AbstractProkaryotes and free-living nematodes are both very abundant and co-occur in marine environments, but little is known about their possible association. Our objective was to characterize the microbiome of a neglected but ecologically important group of free-living benthic nematodes of the Oncholaimidae family. We used a multi-approach study based on microscopic observations (Scanning Electron Microscopy and FluorescenceIn SituHybridization) coupled with an assessment of molecular diversity using metabarcoding based on the 16S rRNA gene. All investigated free-living marine nematode specimens harboured distinct microbial communities (from the surrounding water and sediment and through the seasons) with ectosymbiosis seemed more abundant during summer. Microscopic observations distinguished two main morphotypes of bacteria (rod-shaped and filamentous) on the cuticle of these nematodes, which seemed to be affiliated toCampylobacterotaandGammaproteobacteria, respectively. Both ectosymbionts belonged to clades of bacteria usually associated with invertebrates from deep-sea hydrothermal vents. The presence of theAprAgene involved in sulfur metabolism suggested a potential for chemosynthesis in the nematode microbial community. The discovery of potential symbiotic associations of a shallow-water organism with taxa usually associated with deep-sea hydrothermal vents, is new for Nematoda, opening new avenues for the study of ecology and bacterial relationships with meiofauna.
Non-invasive detection of DNA methylation states in carcinoma and pluripotent stem cells using Raman microspectroscopy and imaging
Authors: Daum Ruben , Brauchle Eva M. , Berrio Daniel Alejandro Carvajal , Jurkowski Tomasz P. , Schenke-Layland Katja 
Published: May 2019

AbstractDNA methylation plays a critical role in the regulation of gene expression. Global DNA methylation changes occur in carcinogenesis as well as early embryonic development. However, the current methods for studying global DNA methylation levels are invasive and require sample preparation. The present study was designed to investigate the potential of Raman microspectroscopy and Raman imaging as non-invasive, marker-independent and non-destructive tools for the detection of DNA methylation in living cells. To investigate global DNA methylation changes, human colon carcinoma HCT116 cells, which were hypomorphic for DNA methyltransferase 1, therefore showing a lower global DNA methylation (DNMT1−/−cells), were compared to HCT116 wildtype cells. As a model system for early embryogenesis, murine embryonic stem cells were adapted to serum-free 2i medium, leading to a significant decrease in DNA methylation. Subsequently, 2i medium -adapted cells were compared to cells cultured in serum-containing medium. Raman microspectroscopy and imaging revealed significant differences between high- and low-methylated cell types. Higher methylated cells demonstrated higher relative intensities of Raman peaks, which can be assigned to the nucleobases and 5-methylcytosine. Principal component analysis detected distinguishable populations of high- and low-methylated samples. Based on the provided data we conclude that Raman microspectroscopy and imaging are suitable tools for the real-time, marker-independent and artefact-free investigation of the DNA methylation states in living cells.
Accumulation and toxicological effects of nonylphenol in tomato (Solanum lycopersicum L) plants
Authors: Jiang Lei , Yang Yi , Zhang Yong , Liu Ying , Pan Bo , Wang Bingjie , Lin Yong 
Published: May 2019

AbstractNonylphenol (NP) is one of the most worrisome and ubiquitous environmental endocrine disruptors. The tomato is one of the most important agricultural plants in the world. However, little is known about the toxicological effects of NP on tomato crops or the accommodative responses of tomato plants to NP stress. Thus, in this study, relevant tests were performed using pot experiments, and they indicated that when the NP concentration in the soil was elevated from 25 mg kg−1 to 400 mg kg−1, NP was progressively accumulated by the tomato plants. The NP induced growth inhibition and a declined in the total chlorophyll content, and it aggravated membrane lipid peroxidation in tomato plants. When confronted with NP stress, the tomato plants correspondingly induced their antioxidant enzymes via both molecular and protein pathways to relieve the NP-induced oxidative stress. All the above results would be illuminating for developing strategies to address NP-induced damage to agricultural output, food quality and public health.
Experimental study on releasing the unbalanced tension using sliding suspension string with V‐shaped groove for transmission line under ice accretion
Authors: Zhang Da‐Chang , Chen Qian , Han Li‐ting , Chen Yan‐Bin , Huang Feng‐Hua 
Published: May 2019

Unbalanced tension is one of the most important loads for the design of transmission towers under ice accretion in cold regions. A V‐shaped sliding groove, which is a new type of connection for suspension strings, is developed as one of the most effective ways to release unbalanced tension. Eight typical experiments on the V‐shaped sliding groove for releasing exceeding unbalanced tensions in transmission lines were designed and performed. The variation mechanism of unbalanced tensions was investigated, and the calculation theory of the critical sliding angle was verified. The experimental results showed that all specimens under all load cases could effectively simulate to release the unbalanced tension when the unbalanced tension increased to the critical value and the suspension string began to slide. Besides, the variation of conductor tensions for 100 m span could be simulated by adopting the curved orbit developed in this study, and the transmission‐line parameters could be changed easily and the experiment became flexible. On the basis of the comparison of theoretical values with the test results of the conductor tension, the design method and practical use of the curved orbit were reasonable and feasible.