bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exosomes derived from M1 macrophages aggravate neointimal hyperplasia following carotid artery injuries in mice through miR-222/CDKN1B/CDKN1C pathway
Authors: Wang Zeng , Zhu Hong , Shi Hongtao , Zhao Huan , Gao Rifeng , Weng Xinyu , Liu Rongle , Li Xiao , Zou Yunzeng , Hu Kai , Sun Aijun , Ge Junbo 
Published: May 2019

AbstractThe role of M1 macrophages (M1M)-derived exosomes in the progression of neointimal hyperplasia remains unclear now. Using a transwell co-culture system, we demonstrated that M1M contributed to functional change of vascular smooth muscle cell (VSMC). We further stimulated VSMCs with exosomes isolated from M1M. Our results demonstrated that these exosomes could be taken up by VSMCs through macropinocytosis. Using a microRNA array assay, we identified that miR-222 originated from M1M-derived exosomes triggered the functional changes of VSMCs. In addition, we confirmed that miR-222 played a key role in promoting VSMCs proliferation and migration by targeting Cyclin Dependent Kinase Inhibitor 1B (CDKN1B) and Cyclin Dependent Kinase Inhibitor 1C (CDKN1C) in vitro. In vivo, M1M-derived exosomes significantly aggravated neointima formation following carotid artery ligation injury and wire injury and these effects were partly abolished by miR-222 inhibitor 2′OMe-miR-222. Our findings thus suggest that exosomes derived from M1M could aggravate neointimal hyperplasia through delivering miR-222 into VSMCs. Future studies are warranted to validate if the post-injury vascular neointimal hyperplasia and restenosis could be attenuated by inhibiting miR-222.
Superconductor to resistive state switching by multiple fluctuation events in NbTiN nanostrips
Authors: Ejrnaes M. , Salvoni D. , Parlato L. , Massarotti D. , Caruso R. , Tafuri F. , Yang X. Y. , You L. X. , Wang Z. , Pepe G. P. , Cristiano R. 
Published: May 2019

AbstractWe report on measurements of the switching current distributions on two-dimensional superconducting NbTiN strips that are 5 nm thick and 80 nm wide. We observe that the width of the switching current distributions has a non-monotonous temperature dependence, where it is constant at the lowest temperatures up to about 1.5 K, after which it increases with temperature until 2.2 K. Above 2.5 K any increase in temperature decreases the distribution width which at 4.0 K is smaller than half the width observed at 0.3 K. By using a careful analysis of the higher order moments of the switching distribution, we show that this temperature dependence is caused by switching due to multiple fluctuations. We also find that the onset of switching by multiple events causes the current dependence of the switching rate to develop a characteristic deviation from a pure exponential increase, that becomes more pronounced at higher temperatures, due to the inclusion of higher order terms.
The time course of auditory recognition measured with rapid sequences of short natural sounds
Authors: Isnard Vincent , Chastres Véronique , Viaud-Delmon Isabelle , Suied Clara 
Published: May 2019

AbstractHuman listeners are able to recognize accurately an impressive range of complex sounds, such as musical instruments or voices. The underlying mechanisms are still poorly understood. Here, we aimed to characterize the processing time needed to recognize a natural sound. To do so, by analogy with the “rapid visual sequential presentation paradigm”, we embedded short target sounds within rapid sequences of distractor sounds. The core hypothesis is that any correct report of the target implies that sufficient processing for recognition had been completed before the time of occurrence of the subsequent distractor sound. We conducted four behavioral experiments using short natural sounds (voices and instruments) as targets or distractors. We report the effects on performance, as measured by the fastest presentation rate for recognition, of sound duration, number of sounds in a sequence, the relative pitch between target and distractors and target position in the sequence. Results showed a very rapid auditory recognition of natural sounds in all cases. Targets could be recognized at rates up to 30 sounds per second. In addition, the best performance was observed for voices in sequences of instruments. These results give new insights about the remarkable efficiency of timbre processing in humans, using an original behavioral paradigm to provide strong constraints on future neural models of sound recognition.
Compton Scattering of Hermite Gaussian Wave γ Ray
Authors: Maruyama Tomoyuki , Hayakawa Takehito , Kajino Toshitaka 
Published: May 2019

AbstractOne of candidates for the generation mechanism of high linearly polarized γ rays in γ-ray bursts is synchrotron radiations from high energy electrons under strong magnetic fields. If this scenario is true, Hermite Gaussian (HG) wave photons, which are one of high-order Gaussian modes, are also generated by high-order harmonic radiations in strong magnetic fields. The HG wave γ rays propagating along the z-direction have quantum numbers of nodes of nx and ny in the x- and y-directions, respectively. We calculate the differential cross sections for Compton scattering of photons described by HG wave function in the framework of relativistic quantum mechanics. The results indicate that it is possible to identify the HG wave photon and its quantum numbers nx and ny and by measuring the azimuthal angle dependence of differential cross section or the energy spectra of the scattered photon as a function of the azimuthal angle.
Serum Magnesium is associated with Carotid Atherosclerosis in patients with high cardiovascular risk (CORDIOPREV Study)
Authors: Rodríguez-Ortiz M. Encarnación , Gómez-Delgado Francisco , Arenas de Larriva Antonio P. , Canalejo Antonio , Gómez-Luna Purificación , Herencia Carmen , López-Moreno Javier , Rodríguez Mariano , López-Miranda José , Almadén Yolanda 
Published: May 2019

AbstractThis study aimed to ascertain whether there is an independent association between serum magnesium (Mg) and the Carotid Intima-Media Thickness (IMT-CC), a well-accepted atherosclerotic-biomarker surrogate of cardiovascular disease (CVD), in a population with high cardiovascular risk. Serum Mg and traditional atherosclerotic risk factors were recorded in 939 patients (mean age, 59.6 ± 0.3 years, 83.2% men) with coronary heart disease (CHD) enrolled in the CORDIOPREV trial. Serum Mg strongly associated with IMT-CC. Before adjusting for potential confounding factors, IMT-CC decreased by 0.111 ± 0.011 mm per mg/dl increase in serum Mg (p < 0.001). After adjustment, the effect of Mg did not appear mediated through factors related to glucose metabolism, the lipid profile or the mineral metabolism and renal function. Multivariate models showed the lower Mg levels (quartile 1) as a strong independent factor contributing to IMT-CC along with age, sex, SBP, HDL-C, and diuretic use. Logistic regression analysis confirmed the predictive ability of serum Mg to differentiate patients at higher atherosclerotic risk as defined by an IMT-CC ≥ 1.0 mm, yielding a OR for the lower quartile of 10.623 (95%CI 2.311–48.845; P = 0.002) and a ROC-derived cutoff of 1.61 mg/dl. Therefore, our findings outline low serum magnesium as a possible independent risk factor for carotid atherosclerosis.
Thermodynamic reasoning for colossal N supersaturation in austenitic and ferritic stainless steels during low-temperature nitridation
Authors: Sasidhar K. N. , Meka S. R. 
Published: May 2019

AbstractColossal N supersaturation of ferritic as well as austenitic stainless steels during low temperature gaseous nitridation treatments has lately gained much technological significance. However, available thermodynamic models to calculate the N paraequilibrium solubility limits have failed to explain the levels of colossal N supersaturation observed in several cases of nitrided ferritic/austenitic stainless steels. In this work, we show that consideration of N dissolution induced spinodal decomposition is essential in calculating the N paraequilibrium solubility limit for both ferritic and austenitic stainless steels. This modification in the thermodynamic model has led to the successful explanation of the thermodynamic cause for the colossal N supersaturation in ferritic and austenitic stainless steels. Available experimental observations in literature support the occurrence of spinodal decomposition.
Immunostimulatory functions of adoptively transferred MDSCs in experimental blunt chest trauma
Authors: Kustermann Monika , Klingspor Malena , Huber-Lang Markus , Debatin Klaus-Michael , Strauss Gudrun 
Published: May 2019

AbstractMyeloid-derived suppressor cells (MDSCs) expand during inflammation and exhibit immunomodulatory functions on innate and adaptive immunity. However, their impact on trauma-induced immune responses, characterized by an early pro-inflammatory phase and dysregulated adaptive immunity involving lymphocyte apoptosis, exhaustion and unresponsiveness is less clear. Therefore, we adoptively transferred in vitro-generated MDSCs shortly before experimental blunt chest trauma (TxT). MDSCs preferentially homed into spleen and liver, but were undetectable in the injured lung, although pro-inflammatory mediators transiently increased in the bronchoalveolar lavage (BAL). Surprisingly, MDSC treatment strongly increased splenocyte numbers, however, without altering the percentage of splenic leukocyte populations. T cells of MDSC-treated TxT mice exhibited an activated phenotype characterized by expression of activation markers and elevated proliferative capacity in vitro, which was not accompanied by up-regulated exhaustion markers or unresponsiveness towards in vitro activation. Most importantly, also T cell expansion after staphylococcal enterotoxin B (SEB) stimulation in vivo was unchanged between MDSC-treated or untreated mice. After MDSC transfer, T cells preferentially exhibited a Th1 phenotype, a prerequisite to circumvent post-traumatic infectious complications. Our findings reveal a totally unexpected immunostimulatory role of adoptively transferred MDSCs in TxT and might offer options to interfere with post-traumatic malfunction of the adaptive immune response.
Runx2 stimulates neoangiogenesis through the Runt domain in melanoma
Authors: Cecconi Daniela , Brandi Jessica , Manfredi Marcello , Serena Michela , Dalle Carbonare Luca , Deiana Michela , Cheri Samuele , Parolini Francesca , Gandini Alberto , Marchetto Giulia , Innamorati Giulio , Avanzi Francesco , Antoniazzi Franco , Marengo Emilio , Tiso Natascia , Mottes Monica , Zipeto Donato , Valenti Maria Teresa 
Published: May 2019

AbstractRunx2 is a transcription factor involved in melanoma cell migration and proliferation. Here, we extended the analysis of Runt domain of Runx2 in melanoma cells to deepen understanding of the underlying mechanisms. By the CRISPR/Cas9 system we generated the Runt KO melanoma cells 3G8. Interestingly, the proteome analysis showed a specific protein signature of 3G8 cells related to apoptosis and migration, and pointed out the involvement of Runt domain in the neoangiogenesis process. Among the proteins implicated in angiogenesis we identified fatty acid synthase, chloride intracellular channel protein-4, heat shock protein beta-1, Rho guanine nucleotide exchange factor 1, D-3-phosphoglycerate dehydrogenase, myosin-1c and caveolin-1. Upon querying the TCGA provisional database for melanoma, the genes related to these proteins were found altered in 51.36% of total patients. In addition, VEGF gene expression was reduced in 3G8 as compared to A375 cells; and HUVEC co-cultured with 3G8 cells expressed lower levels of CD105 and CD31 neoangiogenetic markers. Furthermore, the tube formation assay revealed down-regulation of capillary-like structures in HUVEC co-cultured with 3G8 in comparison to those with A375 cells. These findings provide new insight into Runx2 molecular details which can be crucial to possibly propose it as an oncotarget of melanoma.
Extreme multi-valence states in mixed actinide oxides
Authors: Epifano E. , Naji M. , Manara D. , Scheinost A. C. , Hennig C. , Lechelle J. , Konings R. J. M. , Guéneau C. , Prieur D. , Vitova T. , Dardenne K. , Rothe J. , Martin P. M. 
Published: May 2019

AbstractTo assure the safety of oxide-fuel based nuclear reactors, the knowledge of the atomic-scale properties of U1−yMyO2±x materials is essential. These compounds show complex chemical properties, originating from the fact that actinides and rare earths may occur with different oxidation states. In these mostly ionic materials, aliovalent cationic configurations can induce changes in the oxygen stoichiometry, with dramatic effects on the properties of the fuel. First studies on U1−yAmyO2±x indicated that these materials exhibit particularly complex electronic and local-structure configurations. Here we present an in-depth study of these compounds, over a wide compositional domain, by combining XRD, XAS and Raman spectroscopy. We provide evidences of the co-existence of four different cations (U4+, U5+, Am3+, Am4+) in U1−yMyO2±x compounds, which nevertheless maintain the fluorite structure. Indeed, we show that the cationic sublattice is basically unaffected by the extreme multi-valence states, whereas complex defects are present in the oxygen sublattice.
Light-promoted synthesis of highly-conjugated crystalline covalent organic framework
Authors: Kim Soyoung , Choi Hee Cheul 
Published: May 2019

AbstractLow electrical conductivity caused by low degree of π-conjugation and structural disorders induces critical limitations in electronic applications of covalent organic frameworks (COFs). Here we focus on improving the electrical properties of COFs by synthesis of highly conjugated and crystalline COF (hcc-COF). Addition of a small amount of water and acetic acid induces the efficient reversible dynamic imine condensation reaction that is required to obtain uniform and crystalline products. Also, simulated sunlight irradiation facilitates the reversible imine condensation reaction, and achieves formation of hcc-COF with high-yield within short reaction time. The obtained hcc-COF has an extended π-conjugated structure along the lateral direction and an inclined stacking structure. The intrinsic electron transport properties along the in-plane direction are evaluated using a hcc-COF film grown on a water surface from a polarity-controlled precursor solution. To the best of our knowledge, our hcc-COF film shows the highest reported electrical conductivity for highly conjugated organic porous polymers.
Increased expression of lncRNA FTH1P3 predicts a poor prognosis and promotes aggressive phenotypes of laryngeal squamous cell carcinoma
Authors: Yuan Haozhan , Jiang Hong , Wang Yanting , Dong Yameng 
Published: May 2019

Abstract
Laryngeal squamous cell cancer (LSCC) is a highly aggressive malignancy in the head and neck region. Recent studies have shown that long noncoding RNAs (lncRNAs) are novel transcripts that play an important role in the progression of LSCC. However, the overall pathophysiological regulation of lncRNAs to LSCC is largely unknown. The present study aimed to determine the clinical significances of lncRNA ferritin heavy chain 1 pseudogene 3 (FTH1P3) and to identify its potential roles in LSCC. Quantitative real-time PCR (qRT-PCR) showed that FTH1P3 expression was significantly up-regulated in LSCC tissues than that in non-neoplastic tissues. High FTH1P3 expression was positively correlated with the poor differentiation, high T classification, positive lymph node metastasis, and advanced clinical stage. Overall survival analysis showed that high levels of FTH1P3 predicted a poor prognosis in LSCC patients. Moreover, elevated expression of FTH1P3 was found to increase LSCC cell proliferation, migration and invasion, and to inhibit cell apoptosis, Conversely, knockdown of FTH1P3 suppressed LSCC cell proliferation, migration and invasion, and induced cell apoptosis. In addition, overexpression of FTH1P3 resulted in an increase in cells in S phase and a decrease in cells in G0/G1 phase, whereas inhibition of FTH1P3 did the opposite effects. Taken together, these results suggested that increased expression of FTH1P3 predicts a poor prognosis and promotes aggressive phenotypes of LSCC by regulating cell proliferation, migration, invasion, apoptosis, and cell cycle, indicating FTH1P3 may serve as a promising therapeutic biomarker for the treatment of LSCC.
Development and validation of a prognostic nomogram for early-onset colon cancer
Authors: Yu Chaoran , Zhang Yujie 
Published: May 2019

Abstract
The present study was to develop a prognostic nomogram to predict overall survival (OS) and cancer-specific survival (CSS) in early-onset colon cancer (COCA, age &lt; 50). Patients diagnosed as COCA between 2004 and 2015 were retrieved from the surveillance, epidemiology, and end results (SEER) database. All included patients were assigned into training and validation sets. Univariate and multivariate analysis were used to identify independent prognostic variables for the construction of nomogram. The discrimination and calibration plots were used to measure the accuracy of the nomogram. A total of 11220 patients were included from the SEER database. The nomograms were established based on the variables significantly associated with OS and CSS using cox regression models. Calibration plots indicated that both nomograms in OS and CSS exhibited high correlation to actual observed results. The nomograms also displayed improved discrimination power than tumor-node-metastasis (TNM) stage and SEER stage both in the training and validation sets. The monograms established in the present study provided an alternative tool to both OS and CSS prognostic prediction compared with TNM and SEER stages.
Molecular profiling of the tumor microenvironment in glioblastoma patients: correlation of microglia/macrophage polarization state with metalloprotease expression profiles and survival
Authors: Gjorgjevski Marko , Hannen Ricarda , Carl Barbara , Li Yu , Landmann Emilie , Buchholz Malte , Bartsch Jörg W. , Nimsky Christopher 
Published: May 2019

Abstract
Due to poor prognosis of glioblastoma (GBM), there is an urgent need to develop new therapeutic strategies. Besides eliminating GBM tumor cells and stem cells, a novel therapeutic approach aims to target Glioma-associated microglia/macrophages (GAMs). We investigated the molecular profile of GAMs correlated with patient prognosis by exploiting M1/M2-like polarization markers in a cohort of 20 GBM patients. Using quantitative PCR (qPCR), the markers CXCL10 (M1) and CCL13 (M2) were validated in human macrophages and applied to a global analysis of GBM tissue. Furthermore, proteinase genes, known to be associated with GBM progression (ADAM8, MMP9, MMP14, ADAM10, ADAM17), were analyzed in correlation to M1/M2 markers. Notably, expression levels of ADAM10 and ADAM17 are significantly correlated with an M1-like phenotype and are positively associated to patient survival. Whilst ADAM8 mRNA expression was equally correlated with M1- and M2-like markers, genes for MMP9 and MMP14 are significantly associated with an M2-like phenotype and association to impaired prognosis in the GBM patient cohort. Thus, we provide a robust and reliable combination of qPCR markers to characterize global microglia/macrophage status and the associated proteinase profiles in GBM patients that can be used to analyze the tumor microenvironment, the patients’ prognosis and preselect those GBM patients for which targeting the microglia/macrophage population by repolarization might be beneficial.
CD14 dictates differential activation of mesenchymal stromal cells through AKT, NF-κB and P38 signals
Authors: Jiang Menghui , Gao Tianlin , Liu Yuansheng , Cao Xue , Li Yanting , Li Jianyu , Liu Yuanjiao , Piao Jinmei 
Published: May 2019

Abstract
Mesenchymal stromal cells (MSCs) widely exist in many tissues and have multiple differentiation potential and immunomodulatory capacities. Recently, MSCs have become promising tools for the treatment of various degenerative disorders and autoimmune diseases. The properties of MSCs could be modified in different microenvironments. Thus, it is important to explore the factors controlling MSC function. The presence of Toll-like receptors (TLRs) in MSCs was demonstrated according to previous studies. Consistently, we also illustrated the expression of TLRs in both murine and human MSCs, and displayed that the expression patterns of TLRs in MSCs from different sources. Furthermore, we explored the role of TLR and TLR signaling pathway in MSCs. Interestingly, activation of TLR4-induced expression of cytokines and some specific genes in MSCs. However, MSCs retained much lower mRNA level compared with macrophages. We explored the expression of CD14 in MSCs from different sources, which played a vital role in TLR4 signaling pathway, and found that MSCs are almost negative for CD14. Moreover, only partial activation of TLR4 signaling pathway was observed in MSCs, with no activation of AKT, NF-κB and P38. Here, in the study we defined TLR expression, function and activation in MSCs, which is critical for designing MSC-based therapies.
High‐resolution FPGA‐pulse width modulation applied to PFC 2 MHz converter using eGaN field effect transistor
Authors: Arbugeri José Augusto , Arbugeri Cesar Augusto , Mussa Samir Ahmad 
Published: May 2019

This study proposes an implementation based on a low‐cost field‐programmable gate array (FPGA) of a high‐resolution pulse width modulation applied on a single‐phase power factor correction (PFC) converter operating with 2 MHz switch frequency. The PFC was implemented using a half‐bridge enhancement‐mode Gallium Nitride (eGaN) field effect transistor with on‐board gate drives (EPC9003 development board) and the MAX10 FPGA.
Design and evaluation of an energy storage system for helicopters
Authors: Klumpner Christian , Rashed Mohamed 
Published: May 2019

This paper presents the design and evaluation of an energy storage system (ESS) for helicopters with the aim to recover the kinetic energy in the rotor available after landing and to be able to control the 270 V DC bus voltage during load disturbances. A study is conducted in order to identify the suitable mix of commercially available energy storage devices with the aim of obtaining the minimum weight, exploring also the possibility to implement a hybrid supercapacitor‐battery system. On the converter side, commercially available Silicon and Silicon‐Carbide devices have been evaluated to achieve also the smallest size/weight.
Multi‐physics design optimisation of a GaN‐based integrated modular motor drive system
Authors: Uğur Mesut , Keysan Ozan 
Published: May 2019

Here, a multi‐physics approach is presented for the design optimisation of an integrated modular motor drive (IMMD). The system is composed of a modular permanent magnet synchronous motor (PMSM) and a GaN‐based modular motor drive power stage. The multi‐physics model includes motor drive inverters and DC‐link capacitor bank (electrical model), stator windings and rotor magnets (electromagnetic model), heat sink (thermal model), and a geometrical model. The main purpose of the design optimisation is to obtain the highest power density possible, which is quite critical in integrated drives. Due to the integrated structure, the system has several interdependencies and parameters are selected based on those relationships. An 8 kW IMMD system design is proposed from the developed optimisation tool and evaluated. The resultant system has a power density of 0.71 kW/lt, drive efficiency of 98.3%, and motor efficiency of 96.6%.