bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inhibition of calcium/calmodulin‐dependent protein kinase kinase (CaMKK) exacerbates impairment of endothelial cell and blood–brain barrier after stroke
Authors: Sun Ping , Bu Fan , Min Jia‐Wei , Munshi Yashasvee , Howe Matthew D. , Liu Lin , Koellhoffer Edward C. , Qi Li , McCullough Louise D. , Li Jun 
Published: November 2018

AbstractBrain microvascular endothelial cells play an essential role in maintaining blood–brain barrier (BBB) integrity, and disruption of the BBB aggravates the ischemic injury. CaMKK (α and β) is a major kinase activated by elevated intracellular calcium. Previously, we demonstrated that inhibition of CaMKK exacerbated outcomes, conversely, overexpression reduced brain injury after stroke in mice. Interestingly, CaMKK has been shown to activate a key endothelial protector, sirtuin 1 (SIRT1). We hypothesized that CaMKK protects brain endothelial cells via SIRT1 activation after stroke. In this study, Oxygen‐Glucose Deprivation (OGD) was performed in human brain microvascular endothelial cells. Stroke was induced by middle cerebral artery occlusion (MCAO) in male mice. Knockdown of CaMKK β using siRNA increased cell death following OGD. Inhibition of CaMKK β by STO‐609 significantly and selectively down‐regulated levels of phosphorylated SIRT1 after OGD. Changes in the downstream targets of SIRT1 were observed following STO‐609 treatment. The effect of STO‐609 on cell viability after OGD was absent, when SIRT1 was concurrently inhibited. We also demonstrated that STO‐609 increased endothelial expression of the pro‐inflammatory proteins ICAM‐1 and VCAM‐1 and inhibition of CaMKK exacerbated OGD‐induced leukocyte‐endothelial adhesion. Finally, intracerebroventricular injection of STO‐609 exacerbated endothelial apoptosis and reduced BBB integrity after 24‐hr reperfusion following MCAO in vivo. Collectively, these results demonstrated that CaMKK inhibition reduced endothelial cell viability, exacerbated inflammatory responses and aggravated BBB impairment after ischemia. CaMKK activation may attenuate ischemic brain injury via protection of the microvascular system and a reduction in the infiltration of pro‐inflammatory factors.
Order anticipation around predictable trades
Authors: Sağlam Mehmet 
Published: November 2018

AbstractI study the presence of order anticipation strategies by examining predictable patterns in large order trades. I construct three simple signals based on child‐order execution patterns and find empirical evidence that stronger signals are correlated with higher execution costs. I use the SEC's (Securities and Exchange Commission's) ban on unfiltered access and increase in noise trading as shocks to order anticipatory activities of algorithmic traders and find that the price impact of predictability is smaller when order anticipation becomes difficult. The empirical findings are mostly consistent with the back‐running theory that predicts delayed price impact as strategic traders learn about large orders gradually.
Effect of 64S bioglass addition on sintering kinetic, flexural strength and osteoblast cell response of yttria‐partially stabilized zirconia ceramics
Authors: Stábile Matías F. , Soubelet Clara G. , Albano María P. , Rosa Adalberto L. , Moreira Spinola de Castro‐Raucci Larissa , Tambasco de Oliveira Paulo 
Published: November 2018

AbstractA quantity of 3 mol% yttria‐partially stabilized zirconia (Y‐TZP) with 10.5 and 19.9 vol% 64S bioglass compacts was sintered at different temperatures up to 1500°C. The influence of 64S glass addition on the sintering kinetic, flexural strength, and osteoblast cell response of Y‐TZP ceramics was investigated. The addition of 64S glass increased the initial sintering rate through the decrease in the activation energy and the increase on the order of diffusion with respect to those previously reported for Y‐TZP. Y‐TZP at 1500°C exhibited the highest flexural strength. Within Y‐TZP ceramics with 64S additions, a maximum flexural strength occurred for 10.5 vol% 64S at 1400°C, its flexural strength was able to approach that of Y‐TZP at 1500°C. The polished sintered surfaces became rougher as the 64S content increased. Cell viability experiments on the less nanoroughness Y‐TZP and Y‐TZP with 10.5 vol% 64S surfaces revealed their good biocompatibility; on the contrary, the high level of nanoroughness of Y‐TZP with 19.9 vol% 64S significantly reduced cell survival. However, the matrix mineralization was not adversely affected by the surface roughness; larger amounts of calcium phosphate phases on Y‐TZP‐19.9 vol% 64S surfaces appeared to promote the osteogenic potential of UMR‐106 cells.
The ABCs of CRISPR in Tephritidae: developing methods for inducing heritable mutations in the genera Anastrepha, Bactrocera and Ceratitis
Authors: Sim S. B. , Kauwe A. N. , Ruano R. E. Y. , Rendon P. , Geib S. M. 
Published: November 2018

AbstractTephritid fruit flies are destructive agricultural pests that are the targets of expensive population eradication and suppression efforts. Genetic pest management is one of the strategies for reducing or eliminating tephritid populations, relying upon the genetic manipulation of insects to render them sterile or capable of transmitting deleterious traits through gene drive. Currently, radiation, chemical mutagenesis, and transgenic techniques are employed to generate agents for genetic pest management, but new methods must be explored and developed for all tephritid pest species. Targeted mutagenesis induced by nonhomologous end join repair of clustered regularly interspaced short palindromic repeats and the CRISPR associated protein 9 (Cas9) (commonly known as CRISPR/Cas9) has been demonstrated to be an efficient method for creating knock‐out mutants and can be utilized to create germline mutations in Tephritidae. In this paper, we describe detailed methods to knockout the white gene in three tephritid species in the genera Anastrepha, Bactrocera and Ceratitis, including the first demonstration of CRISPR/Cas9 induced mutations in the genus Anastrepha. Lastly, we discuss the variables in tephritid systems that directed method development as well as recommendations for performing injections in remote containment facilities with little molecular biology capabilities. These methods and recommendations combined can serve as a guide for others to use in pursuit of developing CRISPR/Cas9 methods in tephritid systems.
The 1918 influenza pandemic in Montevideo: The southernmost capital city in the Americas
Authors: Cristina Juan , Pollero Raquel , Pellegrino Adela 
Published: November 2018

BackgroundFew studies have addressed the impact and dynamics of the 1918‐1919 influenza pandemic in temperate regions of South America.ObjectiveTo identify key factors for influenza onset, spread, and mortality in Montevideo and Uruguay in 1918‐1919.MethodsAn analysis of official national records of the public health system of Uruguay was performed.ResultsFrom November to December of 1918 (spring), a total of 131 deaths due to influenza occurred in Montevideo and a total of 296 deaths accounted from July to September of 1919 (winter) in the same city. The total deaths attributed to influenza in Uruguay in 1918 and 1919 were 926 and 1089, respectively. In contrast, the mean annual mortality attributed to influenza in Uruguay from 1908 to 1917 was 50.9. A pattern of age‐shift in mortality in the two pandemic waves studied was observed.ConclusionsThe results of studies revealed that Montevideo was first hit by the devastating second wave of the pandemic of 1918, arriving Montevideo at the end of the spring of that year. The third wave arrived by July 1919, in the winter season, and in the capital city was as severe as the second one.
Anticancer activity of paroxetine in human colon cancer cells: Involvement of MET and ERBB3
Authors: Jang Won‐Jun , Jung Sung Keun , Vo Tam Thuy Lu , Jeong Chul‐Ho 
Published: November 2018

AbstractThe concept of drug repositioning has recently received considerable attention in the field of oncology. In the present study, we propose that paroxetine can be used as a potent anticancer drug. Paroxetine, one of the selective serotonin reuptake inhibitors (SSRIs), has been widely prescribed for the treatment of depression and anxiety disorders. Recently, SSRIs have been reported to have anticancer activity in various types of cancer cells; however, the underlying mechanisms of their action are not yet known. In this study, we investigated the potential anticancer effect of paroxetine in human colorectal cancer cells, HCT116 and HT‐29. Treatment with paroxetine reduced cell viability, which was associated with marked increase in apoptosis, in both the cell lines. Also, paroxetine effectively inhibited colony formation and 3D spheroid formation. We speculated that the mode of action of paroxetine might be through the inhibition of two major receptor tyrosine kinases – MET and ERBB3 – leading to the suppression of AKT, ERK and p38 activation and induction of JNK and caspase‐3 pathways. Moreover, in vivo experiments revealed that treatment of athymic nude mice bearing HT‐29 cells with paroxetine remarkably suppressed tumour growth. In conclusion, paroxetine is a potential therapeutic option for patients with colorectal cancer.
Parallel plumage colour evolution and introgressive hybridization in wheatears
Authors: Schweizer Manuel , Warmuth Vera , Alaei Kakhki Niloofar , Aliabadian Mansour , Förschler Marc , Shirihai Hadoram , Suh Alexander , Burri Reto 
Published: November 2018

AbstractGenetic and phenotypic mosaics, in which various phenotypes and different genomic regions show discordant patterns of species or population divergence, offer unique opportunities to study the role of ancestral and introgressed genetic variation in phenotypic evolution. Here, we investigated the evolution of discordant phenotypic and genetic divergence in a monophyletic clade of four songbird taxa—pied wheatear (O. pleschanka), Cyprus wheatear (Oenanthe cypriaca), and western and eastern subspecies of black‐eared wheatear (O. h. hispanica and O. h. melanoleuca). Phenotypically, black back and neck sides distinguish pied and Cyprus wheatears from the white‐backed/necked black‐eared wheatears. Meanwhile, mitochondrial variation only distinguishes western black‐eared wheatear. In the absence of nuclear genetic data, and given frequent hybridization among eastern black‐eared and pied wheatear, it remains unclear whether introgression is responsible for discordance between mitochondrial divergence patterns and phenotypic similarities, or whether plumage coloration evolved in parallel. Multispecies coalescent analyses of about 20,000 SNPs obtained from RAD data mapped to a draft genome assembly resolve the species tree, provide evidence for the parallel evolution of colour phenotypes and establish western and eastern black‐eared wheatears as independent taxa that should be recognized as full species. The presence of the entire admixture spectrum in the Iranian hybrid zone and the detection of footprints of introgression from pied into eastern black‐eared wheatear beyond the hybrid zone despite strong geographic structure of ancestry proportions furthermore suggest a potential role for introgression in parallel plumage colour evolution. Our results support the importance of standing heterospecific and/or ancestral variation in phenotypic evolution.
Comparison of endoscopic ultrasound tissue acquisition methods for genomic analysis of pancreatic cancer
Authors: Elhanafi Sherif , Mahmud Nadim , Vergara Norge , Kochman Michael L , Das Koushik K , Ginsberg Gregory G , Rajala Michael , Chandrasekhara Vinay 
Published: November 2018

AbstractBackground and AimTumor genotyping may allow for improved prognostication and targeted therapy for pancreatic ductal adenocarcinoma (PDAC). We aimed to compare endoscopic ultrasonography (EUS) with fine needle aspiration (FNA) to fine needle biopsy (FNB) for obtaining sufficient tissue for genomic analysis and theranostic potential.MethodsA retrospective cohort study of patients that underwent EUS‐FNA or EUS‐FNB with either positive or suspicious cytology for PDAC between March 2016 and December 2017. Demographic, procedural, and cytology data were recorded. Genetic alterations were recorded, and Kaplan–Meier survival curves were calculated.ResultsThe study included 167 patients: 145 patients had FNA and 22 patients underwent FNB. Overall, 117 samples (70.1%) were sufficient for targeted next‐generation sequencing. FNB resulted in a higher proportion of patients with sufficient samples compared with FNA (90.9% vs 66.9%; P = 0.02). In multivariable modeling, only FNB (odds ratio 4.95, 95% confidence interval 1.11–22.05, P = 0.04) was associated with sufficient sampling for genomic testing. FNB was more likely to obtain sufficient tissue from tumors ≤ 3 cm (100% vs 68.4%, P = 0.017) and tumors located in the head/neck of the pancreas (100% vs 63.1%, P = 0.03) compared with FNA. The most commonly identified alterations were in KRAS (88%), TP53 (68%), and SMAD4 (16%).ConclusionsEndoscopic ultrasonography can reliably obtain sufficient tissue from PDAC for targeted genomic sequencing for prognostication and theranostics. FNB should be considered when tumor genotyping is requested, especially for tumors ≤ 3 cm or tumors located in the head/neck of the pancreas.
Entrepreneurship, College, and Credit: The Golden Triangle
Authors: SAMANIEGO ROBERTO M. , SUN JULIANA YU 
Published: November 2018

AbstractWe develop a model to evaluate the aggregate impact of college finance in an environment with entrepreneurship. The calibrated model captures the stylized fact that entrepreneurs with college are more common and more profitable in the United States. The calibration indicates this is mainly because higher labor earnings allow college‐educated agents to ameliorate credit constraints if and when they eventually become entrepreneurs. Changes in financing constraints on entrepreneurs can thus affect college attendance, and changes in financing constraints on college can affect entrepreneurship rates as well.
Annual report of the committee on gynecologic oncology, the Japan Society of Obstetrics and Gynecology: Annual patients report for 2015 and annual treatment report for 2010
Authors: Nagase Satoru , Ohta Tsuyoshi , Takahashi Fumiaki , Enomoto Takayuki ,   The 2017 Committee on Gynecologic Oncology of the Japan Society of Obstetrics and Gynecology
Published: November 2018

AbstractAimTo provide information including the trend of gynecological malignancies in Japan, we hereby present the Annual Patient Report for 2015 and the Annual Treatment Report for 2010 on the outcomes of patients who started treatment in 2010.MethodsThe Japan Society of Obstetrics and Gynecology maintains an annual tumor registry where information on gynecological malignancies from various participating institutions is gathered. The data of patients whose treatment was initiated in 2015 were analyzed retrospectively. Survival of the patients who started treatment in 2010 was analyzed by using the Kaplan–Meier, log‐rank and Wilcoxson tests.ResultsTreatment was initiated in 2015 for 7527 patients with cervical cancer, 10 119 with endometrial cancer, 6424 with ovarian cancer and 2181 with ovarian borderline tumors. This clinicopathological information was summarized as the Patient Annual Report. Prognoses were analyzed across 4309 patients with cervical cancer, 5054 with endometrial cancer and 3423 with ovarian cancer, whose treatment was initiated in 2010. The 5‐year survival rates of the patients with cervical cancer were 92.1%, 74.2%, 52.0%, and 29.8% for stages I, II, III, and IV, respectively. The 5‐year survival rates for the patients with endometrial cancer were 94.3%, 88.8%, 74.0% and 26.6% for stages I, II, III and IV, respectively. The 5‐year survival rates for the patients with ovarian cancer (surface epithelial‐stromal tumors) were 88.5%, 80.1%, 46.3% and 36.2% for stages I, II, III and IV, respectively.ConclusionThe annual tumor report is an important survey that provides knowledge on gynecological malignancy trends in Japan.
The Development of Willingness to Sacrifice and Unmitigated Communion in Intimate Partnerships
Authors: Johnson Matthew D. , Horne Rebecca M. , Neyer Franz J. 
Published: November 2018

Objective: This study examined developmental trajectories of willingness to sacrifice and unmitigated communion, or putting a partner's needs above one's own to the exclusion of the self.Background: Willingness to sacrifice and unmitigated communion have proven important for personal and relationship well‐being, but no prior research has sought to understand their developmental trajectories or the factors that motivate growth in each. We draw from a relational developmental systems perspective to explore these questions.Method: Latent growth curve analyses of German Family Panel data from 3,405 focal participants and their partners surveyed across seven years were used to answer the research questions.Results: Findings revealed each construct followed a declining curvilinear pattern with much variability underlying average trajectories. Women, those with more prior partnerships, and those in longer‐term relationships were less willing to sacrifice and had lower unmitigated communion at baseline. Participants were more willing to sacrifice when participants were more committed and more insecure in their partner's love. Unmitigated communion was higher when they were more committed, when they and their partners were happier with the union, and when household income was lower.
Conclusion: Willingness to sacrifice and unmitigated communion develop in response to sociodemographics, personal history, and both partners' relationship cognitions.
Urbanisation and Agricultural Productivity: Why Did the Splendour of the Italian Cities in the Sixteenth Century Not Lead to Transition?
Authors: Chiarini Bruno , Marzano Elisabetta 
Published: November 2018

SummaryIn this paper, we investigate the relationships among total population, wages and the urban population in the Italian economy during the period 1320‐1870. From the late Middle Ages to the Early Modern age (the Italian Renaissance), the prevailing conditions were those of a poor, mainly agricultural economy with rudimentary technology. However, these centuries witnessed considerable growth of urban centres. The question that drives this paper is why the development of Italian cities in the sixteenth century did not lead to sustained growth. Using a vector autoregression (VAR) model, we provide a picture of a trapped economy in which urbanisation was unable to trigger a persistent process of development because migration to cities had negative consequences for rural marginal productivity. The demand for a young and healthy labour force from the urban sector was not adequately supported by the productivity of the agricultural sector, which suffered from a lack of technological innovation.
Hygienic conditions influence sensitization to Blomia tropicalis allergenic components: Results from the FRAAT birth cohort
Authors: Zakzuk Josefina , Mercado Dilia , Bornacelly Adriana , Sánchez Jorge , Ahumada Velky , Acevedo Nathalie , Caraballo Luis 
Published: November 2018

AbstractBackgroundIn tropical zones, perennial exposure to house dust mite (HDM) allergens and helminth infections is present. Studying the impact of these conditions on the inception and evolution of allergic diseases is necessary to have an accurate view of their natural history. We aimed to evaluate the dynamics of genuine sensitization to Blomia tropicalis and Ascaris in children from the FRAAT birth cohort and the effects of helminth infection, environmental HDM allergen levels, and sociodemographic factors.MethodsChildren were followed up to 6 years old. Specific IgE to recombinant allergens from B. tropicalis (Blo t 5 and Blo t 12) and Ascaris spp (Asc l 3, Asc l 13 and Asc s 1) was measured by ELISA at different time points. Allergen levels were measured in dust when children were 6 months old.ResultsIgE sensitization increased over time up to 3 years old. Correlation among the specific IgE levels to B. tropicalis and Ascaris components is poor at year 1, but coefficients are high and significant (Spearman's rho coefficients >0.70) at year 6. Unhygienic conditions increased the odds of sensitization to B. tropicalis allergenic components. Blo t 5 levels were lower in the poorest. IgE response to Blo t 5 and Blo t 12 was less intense in children with high exposure to Blo t 5 (levels >80th percentile).ConclusionIn this tropical community, the pattern of childhood IgE sensitization is different from that in developing countries and is influenced by the hygienic conditions.
I am more than a mom: Stories of parental leave during the transition to motherhood in Canada
Authors: Paterson Stephanie , Hebblethwaite Shannon , Trussell Dawn , Evans Meredith , Xing Trisha 
Published: November 2018

AbstractThe transition to motherhood is a time of tremendous change, during which maternal subjects experience important shifts in their relationships with family, friends, employers, and state. Parental leave policies have potential to mitigate some of these changes, contributing positively to health and economic outcomes. Indeed, such policies have been identified as essential for achieving gender equality, serving to address both recognition and redistributive claims. In this paper, we explore parental leave policy through the lens of first time mothers in Canada, where two distinct policy regimes coexist. Using narrative analysis, we examine the ways in which the transition to motherhood is embodied and enacted through policy experiences. The stories we uncover, stories of helplessness, of control and resistance, and of stymied progress, illuminate how leave policies differently position maternal subjects in complex social settings.
“We do STEM”: Unsettled conceptions of STEM education in middle school S.T.E.M. classrooms
Authors: Kloser Matthew , Wilsey Matthew , Twohy Kyra E. , Immonen Audrey D. , Navotas Ariel C. 
Published: November 2018

AbstractSTEM education has received attention both as a reform pedagogy and as a public means for economic growth and national security. Yet, despite this attention, clarity about what “counts” as STEM and how the individual disciplines relate to an integrated approach remains ambiguous. This article elicits the conceptions about what constitutes STEM education from middle grade teachers across the United States who teach a STEM discipline. Based on coded interviews and drawn conceptual models, participating teachers were more likely to describe essential aspects of integrated STEM found in the literature, but when asked to draw their conception of STEM education in their context, they often represented less ambitious conceptions. Furthermore, nontraditional subjects like engineering and technology were sometimes represented in subordinate ways to traditional subjects like science and mathematics. These findings have implications for preparing teachers to engage students in integrated STEM learning experiences as well as policy implications for what requirements must be in place to fund new STEM learning opportunities.