bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrin α7 high expression correlates with deteriorative tumor features and worse overall survival, and its knockdown inhibits cell proliferation and invasion but increases apoptosis in breast cancer
Authors: Bai Xiaorong , Gao Chen , Zhang Lifeng , Yang Suisheng 
Published: July 2019

AbstractBackgroundThis study aimed to investigate the correlation of integrin α7 (ITGA7) expression with clinical/pathological characteristics and overall survival (OS), and its knockdown on inhibiting cell activities in breast cancer.MethodsA total of 191 breast cancer patients underwent surgery were retrospectively reviewed, and ITGA7 expression in tumor tissues was determined by immunofluorescence and real‐time quantitative polymerase chain reaction. Patients’ clinical/pathological data were recorded, and OS was calculated. In vitro, control shRNA and ITGA7 shRNA plasmids were transfected into MCF7 cells to evaluate the influence of ITGA7 knockdown on cell proliferation, apoptosis, and invasion.ResultsNinety‐two (48.2%) patients presented with ITGA7 high expression, and 99 patients (51.8%) presented with ITGA7 low expression. ITGA7 expression was positively correlated with T stage, tumor‐node metastasis (TNM) stage, and pathological grade. Kaplan‐Meier curves showed that ITGA7 high expression was associated with shorter OS, and multivariate Cox's proportional hazards regression displayed that ITGA7 high expression was an independent predictive factor for poor OS. Moreover, in vitro experiments disclosed that cell proliferation (by Cell Counting Kit‐8 assay) and cell invasion (by Matrigel invasion assay) were reduced, while cell apoptosis rate (by Annexin V/propidium iodide assay) was enhanced by ITGA7 knockdown in MCF‐7 cells.ConclusionIntegrin α7 high expression correlates with increased T stage, TNM stage, and pathological grade as well as worse OS, and its knockdown enhances cell apoptosis but inhibits cell proliferation and invasion in breast cancer.
Co‐sensitization and cross‐reactivity of Blomia tropicalis with two Dermatophagoides species in Guangzhou, China
Authors: Liu Xiaoqing , Zheng Peiyan , Zheng Song Guo , Zhai Yingying , Zhao Xiaoming , Chen Yin , Cai Chuanxu , Wu Zehong , Huang Zhifeng , Zou Xiangwei , Liao Chenxi , Sun Baoqing 
Published: July 2019

AbstractBackgroundContradictory results have been reported previously in the analyses of cross‐reactivity among Blomia tropicalis (Blo t), Dermatophagoides pteronyssinus (Der p), and Dermatophagoides farinae (Der f). This study aims to investigate the characteristics of co‐sensitization and the IgE cross‐reactivity among them and attempts to identify whether patients are sensitized to Blo t due to cross‐reaction or true sensitization.MethodsSpecific IgE (sIgE) in the sera from 1497 allergenic patients was determined by ImmunoCAP. Cross‐reactivity was analyzed and determined by sIgE inhibition with 21 sera samples.ResultsAround 85.50% of patients were sensitized to Der p, 85.37% of patients were sensitized to Der f, and 71.54% of patients were sensitized to Blo t. Further, 70.14% of patients were co‐sensitized to Blo t, Der p, and Der f, and only seven patients were sensitized solely to Blo t. With increasing sIgE levels for Blo t, the positive rates of severe‐level (class 5‐6) co‐sensitization to Der p or Der f significantly increased. Blo t was moderately associated with Der p and Der f, with correlation coefficients of 0.6998 and 0.6782, respectively. Der p and Der f inhibited IgE binding to Blo t more strongly than Blo t inhibited IgE binding to Der p or Der f in the patient groups CBlo t < CDer p and CBlo t < CDer f.ConclusionsThis study has established valuable information about the co‐sensitization and cross‐reactivity of Blo t with two Dermatophagoides species (Der p and Der f) and helps to provide adequate diagnosis and treatment of the mite‐allergic patients.
Association between subclinical hypothyroidism and metabolic disorders: A retrospective chart review study in an emerging university hospital
Authors: Aldossari Khaled , Al‐Ghamdi Sameer , Al‐Zahrani Jamaan , Al Jammah Anwar , Alanazi Bader , Al‐Briek Abdulilah , Alanazi Mohammad 
Published: July 2019

AbstractBackgroundSubclinical hypothyroidism is defined as an increase in serum levels of Thyroid Stimulating Hormone (TSH) above the normal range, without alteration of total T4 concentrations that is reported to have association with various metabolic conditions. The study aimed to investigate any association between subclinical hypothyroidism and metabolic disorders in Al Kharj city.MethodsThis is a cross‐sectional study that included review of patients’ charts from prince Sattam bin Abdul‐Aziz University, Al Kharj, Saudi Arabia, from August 1 to November 30, 2016. Data were analyzed with SPSS version 21. Descriptive statistics were obtained as frequencies. Pearson chi‐square analysis was used to assess any differences between disease status and study variables. P‐value < 0.05 was considered significant.ResultsThe mean age was 30.65 ± 13.3 with a female predominance. The average BMI was 29.5 ± 7.71; 46 (11.5%) had hypertension, 52 (46.8%) had diabetes, 173 (44%) had anemia, and 192 (56%) had vitamin D deficiency. Due to increased TSH levels, male gender had higher prevalence of subclinical hypothyroidism with P‐value < 0.001 and 0.011, respectively.ConclusionSubclinical hypothyroidism is a significant topic worldwide whose prevalence is rising. In this study, we could not find any significant association between subclinical hypothyroidism and metabolic disorder. Further longitudinal studies with large sample size are needed to study this association.
Recent advancements in the study of breast cancer exosomes as mediators of intratumoral communication
Authors: Groza Monica , Zimta Alina–Andreea , Irimie Alexandru , Achimas‐Cadariu Patriciu , Cenariu Diana , Stanta Giorgio , Berindan‐Neagoe Ioana 
Published: July 2019

AbstractBreast cancer is a heterogeneous disease, with a morbidity rate of 27.8% and a mortality rate of 15% among women population worldwide. Understanding how this cancer develops and the mechanisms behind tumor progression and chemoresistance is of utmost importance. Exosomes mediate communication in a population of heterogeneous tumoral cells. They have a cargo composed of oncogenes and oncomiRs which change the transcriptomic scenario of their targeted cells and activate numerous tumor‐promoting signaling pathways. Exosomes secreted by breast cancer cells lead to enhanced cell proliferation, replicative immortality, angiogenesis, invasion, migration, and chemoresistance. Studying exosomes from this perspective offers more in depth understanding of breast malignancy and may aid in the future development of early diagnostic, prognostic and therapeutic options. We present the latest findings in this area and offer practical solutions which may further stimulate the much‐needed research of exosome in breast cancer.
Sequential treatment with bicarbonate and low‐temperature to potentiate both biomass and lipid productivity in Nannochloropsis oceanica
Authors: Yuan Wasiqi , Ma Yuhan , Wei Wei , Liu Wanjun , Ding Yu , Balamurugan Srinivasan 
Published: July 2019

AbstractBACKGROUNDMicroalgal lipid overproduction has been achieved greatly by stress treatments, which drastically impede cellular biomass and overall productivity, hence providing the impetus to develop a viable strategy to uncouple lipid enhancement from stress associated growth impairment.RESULTSWe designed a sequential strategy in Nannochloropsis oceanica, to enhance cellular biomass and carbon sink by providing optimized bicarbonate source during the growth phase, followed by low‐temperature treatment to harness the increased cellular biomass and carbon precursors for lipid hyperaccumulation. Provision of bicarbonate (1.0 g/L) remarkably promoted cellular physiological properties, which in turn enhanced cellular biomass and photosynthetic carbon sink. Subsequent low‐temperature treatment (16 °C) significantly redirected the carbon flux towards lipogenesis and significantly altered fatty acid composition. The designed strategy resulted in significant enhancement of lipid productivity by 2.9‐fold than that of control cells and resulted in lipid accumulation up to 57% of dry cell weight without impeding cellular biomass. Interestingly, the sequential treatment altered fatty acid profile, particularly a 8.8‐fold increase in the content of eicosapentaenoic acid with health significance.CONCLUSIONOur study exemplifies the feasible strategy to potentiate microalgal biomass, and also to circumvent stress associated growth impairment and consequent algal lipid productivity. © 2019 Society of Chemical Industry
Out‐colourings of digraphs
Authors: Alon Noga , Bang‐Jensen Jørgen , Bessy Stéphane 
Published: July 2019

AbstractWe study vertex colourings of digraphs so that no out‐neighbourhood is monochromatic and call such a colouring an out‐colouring. The problem of deciding whether a given digraph has an out‐colouring with only two colours (called a 2‐out‐colouring) is ‐complete. We show that for every choice of positive integers there exists a ‐strong bipartite tournament, which needs at least colours in every out‐colouring. Our main results are on tournaments and semicomplete digraphs. We prove that, except for the Paley tournament , every strong semicomplete digraph of minimum out‐degree at least three has a 2‐out‐colouring. Furthermore, we show that every semicomplete digraph on at least seven vertices has a 2‐out‐colouring if and only if it has a balanced such colouring, that is, the difference between the number of vertices that receive colour 1 and colour 2 is at most one. In the second half of the paper, we consider the generalization of 2‐out‐colourings to vertex partitions of a digraph so that each of the three digraphs induced by respectively, the vertices of , the vertices of and all arcs between and , have minimum out‐degree for a prescribed integer . Using probabilistic arguments, we prove that there exists an absolute positive constant so that every semicomplete digraph of minimum out‐degree at least has such a partition. This is tight up to the value of .
Efficacy of direct‐acting antivirals: UK real‐world data from a well‐characterised predominantly cirrhotic HCV cohort
Authors: Macken Lucia , Gelson William , Priest Matthew , Abouda George , Barclay Stephen , Fraser Andrew , Healy Brendan , Irving Will , Verma Sumita 
Published: July 2019

AbstractDirect‐acting antivirals (DAAs) have revolutionised the management of chronic hepatitis C virus (HCV) infection. We describe UK real‐world DAA experience. Individuals commencing HCV treatment containing a DAA regimen (Mar 2014‐Nov 2016), participating in the National HCV Research UK (HCVRUK) Cohort Study were recruited from 33 UK HCV centers. The data were prospectively entered at sites onto a centralised database. The data were reported as median (Q1‐Q3). Of the 1448 treated patients, 1054 (73%) were males, the median age being 54 years (47‐60), 900 (62%) being genotype 1 and 455 (31%) genotype 3. The majority, 887 (61%) had cirrhosis, and 590 (41%) were treatment‐experienced. DAA regimens utilised: genotype1 sofosbuvir (SOF)/Ledipasvir/±Ribavirin (625/900, 69%) and Ombitasvir/Paritaprevir/Dasabuvir/±RBV (220/900, 24%), and in genotype 3 SOF/Daclatasvir + RBV (256/455, 56%) and SOF/pegylated interferon/RBV (157/455, 35%). Overall, 1321 (91%) achieved sustained virological response (SVR12), genotype 1 vs 3, 93% vs 87%, P < .001. Prior treatment, presence of cirrhosis and treatment regimen did not impact SVR12. Predictors of treatment failure were genotype 3 infection, OR, 2.015 (95% CI: 1.279‐3.176, P = .003), and male sex, OR, 1.878 (95% CI: 1.071‐3.291, P = .028). Of those with hepatic decompensation at baseline (n = 39), 51% (n = 20) recompensated post‐treatment, lower baseline serum creatinine being associated with recompensation (P = .029). There were two liver‐related deaths, both having decompensated disease. This real‐world UK data, comprising of a predominantly cirrhotic HCV genotype 1/3 cohort, confirms DAA efficacy with an overall 91% SVR12, with 51% recompensating post‐treatment. Genotype 3 infection was a predictor of treatment failure.
Impacts of climate change on characteristics of daily‐scale rainfall events based on nine selected GCMs under four CMIP5 RCP scenarios in Qu River basin, east China
Authors: Gao Chao , Booij Martijn J. , Xu Yue‐Ping 
Published: July 2019

AbstractClimate change significantly influences characteristics of rainfall events including rainfall depth, rainfall duration, inter‐event time and temporal patterns that directly affect water resources management, flood defence and hydraulic structure design. In this study, a framework is proposed to analyse daily‐scale rainfall event characteristics based on global climate model (GCM) simulations. This framework includes bias correction of raw GCM‐simulated rainfall series, selection of good‐performing bias‐corrected GCMs based on the mean absolute percentage error (MAPE) and evaluation of selected GCMs' skills in simulating rainfall event characteristics and finally assessment of changes in rainfall event characteristics in the future. In this study, 17 GCMs, four representative concentration pathways (i.e., RCP2.6, RCP4.5, RCP6.0 and RCP8.5) and two future periods (i.e., 2041–2070 and 2071–2100) are considered. After bias correction of the GCMs using the monthly‐scale double gamma distribution, 9 out of 17 GCMs with MAPE values smaller than 20% in the historical period 1971–2000 are selected. In general, these selected GCMs well capture the rainfall characteristics of different rainfall event classes. The multi‐model ensembles suggest that compared to the historical period, the frequency of rainfall events with an extreme depth, short duration and long inter‐event time will increase in the two future periods and the change in 2071–2100 is generally larger than that in 2041–2070, indicating that more extreme climate conditions may occur in Qu River basin in the future. Moreover, the temporal patterns of heavy rainfall events will become more non‐uniform with more concentrated peak rainfall. The frequency of the delayed rainfall type (i.e., peaks occurring at the end of the rainfall event) will increase in the future, which can probably cause more severe floods and is very detrimental to flood defence in this study area.
Hydrolysis of chlorogenic acid in apple juice using a p‐coumaryl esterase of Rhizoctonia solani
Authors: Siebert Mareike , Berger Ralf Günter , Pfeiffer Franziska 
Published: July 2019

AbstractBACKGROUNDApple juice is rich in polyphenolic compounds, especially in chlorogenic acid. A sour and bitter taste has been attributed to the compound. Chlorogenic acid in coffee powder was quickly hydrolysed by a p‐coumaryl esterase of Rhizoctonia solani (RspCAE) at its optimal pH of 6.0. It was unknown, however, if RspCAE would also degrade chlorogenic acid under the strongly acidic conditions (pH 3.3) present in apple juice.RESULTSTreatment of apple juice with RspCAE led to a chlorogenic acid degradation from 53.38 ± 0.94 mg L−1 to 21.02 ± 1.47 mg L−1. Simultaneously, the caffeic acid content increased from 6.72 ± 0.69 mg L−1 to 19.33 ± 1.86 mg/L−1. The aroma profile of the enzymatically treated sample and a control sample differed in only one volatile. Vitispirane had a higher flavour dilution factor in the treated juice. Sensory analysis showed no significant difference in the taste profile ( p < 0.05).CONCLUSIONThese results demonstrated a high stability and substrate specificity of RspCAE. An increase in caffeic acid and a concurrent decrease in chlorogenic acid concentration may exert a beneficial effect on human health. © 2019 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
Evaluating the metabolic perturbations in Mangifera indica (mango) ripened with various ripening agents/practices through gas chromatography ‐ mass spectrometry based metabolomics
Authors: Ganneru Sireesha , Shaik Hussain , Peddi Kiranmayi , Mudiam Mohana Krishna Reddy 
Published: July 2019

AbstractMangifera indica L. (mango) is said to be the king of fruits due to its rich nutritional properties and mainly originates from the Indian sub‐continent. The consumption pattern of the mangoes has increased drastically, due to which, many ripening practices/agents were used to make it ready‐to‐eat fruit or juice for the consumers. The fruit quality and metabolic composition are said to be altered due to different ripening agents/practices. The present communication mainly deals to understand the metabolic perturbations in mango fruits due to different ripening practices/agents (room temperature ripening, ethylene, and calcium carbide) using gas chromatography ‐ mass spectrometry based metabolomics. The partial least square‐discriminant analysis has found 16 differential metabolites for different ripening agents/practices which are belong to the classes of amino acids, fatty acids, sugars, and polyols. Four metabolic pathways were found to alter in the fruit metabolome due to different ripening agents/practices. Fructose, glucose, and galactose were found to be significantly up‐regulated due to calcium carbide ripening in comparison to other ripening agents/practices. Overall findings from the present study advocates that mass spectrometry based metabolomics can be valuable tool to understand the fruit quality and safety with respect to consumer health.
Is Ultrasound‐Guided Core Biopsy as Safe as Fine‐Needle Aspiration, and Does It Add Significantly to the Diagnosis of Suspected Peritoneal Malignancy?
Authors: Kipper Benjamin , McGahan John P. , Loehfelm Thomas W. , Fananapazir Ghaneh 
Published: July 2019

ObjectivesWe undertook this retrospective review to compare the safety and diagnostic yield of core biopsy (CB) compared to fine‐needle aspiration (FNA) in patients with suspected peritoneal malignancy.MethodsThis retrospective study included 35 patients who underwent ultrasound (US)‐guided percutaneous biopsy of a peritoneal mass. Success rates of US‐guided biopsy of these masses using the CB technique, FNA, or the combination of the two were compared. Outcomes of tissue adequacy, the final pathologic diagnosis, and complications were recorded. The binary outcome variable was adequate tissue obtained.ResultsAdequate specimens were obtained in 94% (33 of 35) of the cases. There were 19 CBs, with 100% of samples sufficient for diagnosis (19 of 19). Thirty‐one FNAs were performed, of which 7 were insufficient, with a diagnostic yield of 77% (24 of 31). There was a statistically significant difference between FNA and CB in providing more adequate tissue for diagnosis in our population (P = .035). There were no significant complications in either group.ConclusionsThe use of the CB technique when performing US‐guided percutaneous biopsy of peritoneal masses provides better tissue for diagnosis compared to FNA. Additional benefits of CB, including genomic testing and tumor subtyping, make this technique a good addition to FNA, without significant complications.
Endoscope‐assisted transoral accessory parotid mass excision: Multicenter prospective observational study
Authors: Kim Jin Pyeong , Lee Dong Kun , Moon Jeong Hwan , Ryu Jun Sun , Woo Seung Hoon 
Published: July 2019

ObjectiveTransoral surgery is gaining favor because it has the advantage of leaving no scar after surgery. The aim of this study was to evaluate the technical feasibility and safety of endoscope‐assisted transoral accessory parotid mass excision.Study DesignMulticenter, prospective, observational study.MethodsThis study was designed as a 7‐year, prospective, multicenter evaluation of endoscope‐assisted transoral accessory parotid mass excision. Clinical outcomes and complications related to the procedures were evaluated in patients.ResultsTwenty patients underwent endoscope‐assisted transoral accessory parotid mass excisions, and 22 patients underwent conventional parotidectomy approach excisions. There was no significant difference with respect to overall demographic characteristics between the groups. However, the operation times were shorter in the transoral approach group (P = 0.001), and cosmetic satisfaction was much better in the transoral group (P < 0.001).ConclusionEndoscope‐assisted transoral accessory parotid mass excision is a potentially safe and effective procedure with excellent outcomes.Level of Evidence2Laryngoscope, 130:1218–1226, 2020
Copolymerization of Pentafluorophenylmethacrylate with Hydrophilic Methacrylamide Monomers Induces Premature Hydrolytic Cleavage
Authors: Heraud Clemence , Basuki Johan , Hughes Timothy C. , Mueller Michael 
Published: July 2019

AbstractActive ester polymers are commonly used for fast development of novel polymer libraries, but they require post‐polymerization modification, which is not atom‐efficient or economical. In order to more efficiently produce 2‐hydroxypropyl methacrylamide (HPMAm) libraries, it would be advantageous to perform a direct copolymerization with active ester monomers. In this work, the synthesis of copolymer libraries of pentafluorophenyl methacrylate (PFPMA) and the hydrophilic monomer HPMAm is investigated. Surprisingly, HPMAm induces premature hydrolytic cleavage of PFPMA, which occurs during polymerization and depends on the HPMAm/PFPMA feed ratio. Copolymerization of PFPMA with N‐isopropylmethacrylamide and the methacrylate monomers 2‐hydroxypropylmethacrylate and N‐isopropylmethacrylate reveals that the hydrolytic cleavage is promoted by copolymerization with methacrylamides only. By switching from a thermal‐ to a light‐based initiator and lowering the reaction temperature, premature hydrolytic cleavage of PFPMA is avoided and allows direct copolymerization of HPMAm together with PFPMA to create polymer libraries for biomaterial screening.
Novel GLI3 variant causes Greig cephalopolysyndactyly syndrome in three generations of a Lithuanian family
Authors: Siavrienė Evelina , Mikštienė Violeta , Radzevičius Darius , Maldžienė Živilė , Rančelis Tautvydas , Petraitytė Gunda , Tamulytė Giedrė , Kavaliauskienė Ingrida , Šarkinas Laurynas , Utkus Algirdas , Kučinskas Vaidutis , Preikšaitienė Eglė 
Published: July 2019

AbstractBackgroundPreaxial polydactyly type IV, also referred as polysyndactyly, has been described in a few syndromes. We present three generations of a family with preaxial polydactyly type IV and other clinical features of Greig cephalopolysyndactyly syndrome (GCPS).Methods and resultsSequencing analysis of the GLI3 coding region identified a novel donor splice site variant NC_000007.14(NM_000168.6):c.473+3A>T in the proband and the same pathogenic variant was subsequently identified in other affected family members. Functional analysis based on Sanger sequencing of the proband's complementary DNA (cDNA) sample revealed that the splice site variant c.473+3A>T disrupts the original donor splice site, thus leading to exon 4 skipping. Based on further in silico analysis, this pathogenic splice site variant consequently results in a truncated protein NP_000159.3:p.(His123Argfs*57), which lacks almost all functionally important domains. Therefore, functional cDNA analysis confirmed that the haploinsufficiency of the GLI3 is the cause of GCPS in the affected family members.ConclusionDespite the evidence provided, pathogenic variants in the GLI3 do not always definitely correlate with syndromic or nonsyndromic clinical phenotypes associated with this gene. For this reason, further transcriptomic and proteomic evaluation could be suggested.