bluuebunny/crossref_metadata_2025_split huggingface dataset:
Peer Educator Dan Komunikator Dapat Meningkatkan Pengetahuan Dan Sikap Remaja Tentang Pernikahan Dini Di SMA Negeri 11 Kota Bengkulu
Authors: Defitri Ayu , Simanjuntak, SKM, MKM Dr. Betty Yosephin , Lagora Reka 
Published: November 2024

Latar Belakang: Pernikahan usia dini seringkali dilakukan remaja yang disebabkan berbagai faktor. Salah satunya pengetahuan dan sikap remaja tentang pernikahan dini masih bervariasi. Tujuan: Mengetahui perubahan pengetahuan dan sikap tentang pernikahan dini melalui peer educator dan komunikator di SMA Negeri 11 Kota Bengkulu. Metode: Penelitian ini adalah penelitian kuantitatif dengan rancangan kuasi eksperimen dengan pre-test dan post-test satu kelompok.Setiap perlakuan diberikan berupa edukasi melalui peer educator dan komunikator pada masing-masing kelompok. Hasil: Hasil analisis uji statistik untuk melihat rerata pengetahuan responden pada kelompok peer educator (88,71) kelompok komunikator (80,97) selisih mean rank (7,74). Hasil analisi uji Mann Whitney diperoleh p=0,000 artinya bahwa skor pengetahuan responden pada kelompok peer educator lebih tinggi dibandingkan dengan kelompok komunikator. Hasil uji statistik rerata sikap pada kelompok peer educator (37,29) kelompok komunikator (34,94) selisih mean rank (2,35). Hasil Independent T test p=0,000 artinya peningkatan skor sikap lebih tinggi pada kelompok peer educator dibanding kelompok komunikator. Kesimpulan: Temuan ini menegaskan pentingnya pemberian edukasi yang dilakukan oleh peer educator dan komunikator. Namun lebih efektif untuk remaja penyampaian melalui peer educator.
PENGARUH VARIASI PERBANDINGAN SAMPAH PLASTIK JENIS LDPE DAN PS TERHADAP VOLUME DAN NILAI KALOR MINYAK PADA PROSES PIROLISIS
Authors: EFITA NADINE ANGGRAINI  , BASUKI  , MOHAMMAD MUNIB ROSADI  , DIAN ANISA ROKHMAHWATI  
Published: November 2024

Sampah plastik yang sangat umum ditemui di lingkungan Universitas Hasyim Asy’ari Tebuireng Jombang yaitu plastik pembungkus suatu makanan atau minuman. Sampah plastik tersebut termasuk ke dalam plastik jenis LDPE dan PS. Konversi sampah plastik menjadi bahan bakar alternatif dilakukan melalui proses pirolisis. Pirolisis adalah proses dimana bahan diuraikan pada suhu tinggi tanpa adanya atau terbatasnya udara. Tujuan penelitian ini adalah untuk mengetahui hasil dari pengaruh variasi berat sampah plastik terhadap volume dan nilai kalor minyak pada proses pirolisis. Metode penelitian yang digunakan adalah metode penelitian eksperimental, dalam metode ini menggunakan variasi perbandingan berat sampah plastik LDPE:PS yaitu: 100%:0%; 70%:30%; 50%:50%; 40%:60% dan 0%:100% dengan total berat sampah plastik sebesar 600 gram yang diolah menggunakan teknik komparasi. Jenis penelitian ini menggunakan jenis penelitian kuantitatif. Pengujian ini dilakukan dengan proses pirolisis selama 120 menit dengan temperatur pembakaran 100 dan temperatur kondensor 10. Hasil dari penelitian ini dapat diketahui bahwa variasi LDPE 0% : PS 100% menghasilkan rata-rata volume minyak yang lebih banyak dibandingkan variasi lainnya sebesar 28,67 ml. Sedangkan variasi LDPE 100% : PS 0% menghasilkan rata-rata nilai kalor minyak yang lebih tinggi dibandingkan variasi lainnya 36197,75967 . Diketahui nilai (Sig.) untuk pengaruh variasi berat sampah plastik terhadap volume minyak pada proses pirolisis adalah 0,048 sehingga dapat disimpulkan bahwa ada pengaruh yang signifikan. Serta dapat diketahui nilai (Sig.) untuk pengaruh variasi berat sampah plastik terhadap nilai kalor minyak pada proses pirolisis adalah 0,742 sehingga dapat disimpulkan bahwa tidak ada pengaruh yang signifikan.
Immunological characteristics in elderly COVID-19 patients: a post-COVID era analysis
Authors: Li Yunhui , Chen Yuan , Liang Jing , Wang Yajie 
Published: November 2024

BackgroundAdvanced age is a primary risk factor for adverse COVID-19 outcomes, potentially attributed to immunosenescence and dysregulated inflammatory responses. In the post-pandemic era, with containment measures lifted, the elderly remain particularly susceptible, highlighting the need for intensified focus on immune health management.MethodsA total of 281 elderly patients were enrolled in this study and categorized based on their clinical status at the time of admission into three groups: non-severe (n = 212), severe survivors (n = 49), and severe non-survivors (n = 20). Binary logistic regression analysis was employed to identify independent risk factors associated with disease severity and in-hospital outcomes. The diagnostic performance of risk factors was assessed using the receiver operating characteristic (ROC) curves. Kaplan-Meier survival analysis and log-rank test were utilized to compare the 30-day survival rates. Furthermore, the transcriptomic data of CD4+ T cells were extracted from Gene Expression Omnibus (GEO) database. Gene Set Enrichment Analysis (GSEA) was applied to reveal biological processes and pathways involved.ResultsIn the comparison between severe and non-severe COVID-19 cases, significant elevations were observed in the neutrophil-to-lymphocyte ratio (NLR), C-reactive protein (CRP), and Serum Amyloid A (SAA) levels, concurrent with a notable reduction in CD8+ T cells, CD4+ T cells, natural killer (NK) cells, and monocytes (all p &lt; 0.05). CD4+ T cells (OR: 0.997 [0.995-1.000], p&lt;0.05) and NLR (OR: 1.03 [1.001-1.060], p&lt;0.05) were independent risk factors affecting disease severity. The diagnostic accuracy for COVID-19 severity, as measured by the area under the curve (AUC) for CD4+ T cells and NLR, was 0.715 (95% CI: 0.645-0.784) and 0.741 (95% CI: 0.675-0.807), respectively. Moreover, patients with elevated NLR or IL-6 levels at admission exhibited significantly shorter survival times. Gene Set Enrichment Analysis (GSEA) revealed several biological pathways that are implicated in the regulation of immune responses and metabolic processes.ConclusionsLymphocytopenia and the cytokine storm onset are significant predictors of an unfavorable prognosis in elderly patients. The decrease in CD4+ T cells among elderly patients is detrimental to disease recovery, and the biological pathways regulated by these cells could potentially heighten vulnerability to SARS-CoV-2 infection, thereby exacerbating the development of associated complications.
Consumer-oriented (patient and family) outcomes from nursing in genomics: a scoping review of the literature (2012–2022)
Authors: Keels Jordan N. , Thomas Joanne , Calzone Kathleen A. , Badzek Laurie , Dewell Sarah , Murthy Vinaya , O’Shea Rosie , Tonkin Emma T. , Dwyer Andrew A. 
Published: November 2024

IntroductionGenomics is a lifespan competency that is important for improving health outcomes for individuals, families, and communities. Nurses play a key role in genomic healthcare and realizing the potential of the genomic era.MethodsWe aimed to chart the current state of genomics in nursing by conducting a systematic scoping review of the literature in four databases (2012–2022). We categorized included articles using the Cochrane Collaboration outcome domains/sub-domains and identify key topical areas.ResultsOf 8532 retrieved articles, we identified 67 articles on ‘consumer-oriented outcomes’ (patient and family) for analysis. Identified articles primarily centered on themes of genetic testing and screening. Most studies reported non-interventional studies 39/67 (58%) and more than half were from the U.S.A. 34/67 (51%). Six of nine subdomains were reported on. The “patient involvement in care” subdomain was the most commonly reported subdomain (17/67, 25%) while “treatment outcomes” had the fewest reports (5/67, 8%). Overall, consumers (i.e., patients and families) had high satisfaction with nurse-led interventions.DiscussionSynthesizing findings revealed key knowledge gaps and unmet patient informational needs around genetic testing and decision support. There are opportunities for interprofessional collaboration between nursing and genetic counseling to meet the mounting demand for genomic healthcare and develop more person-centered approaches to genetic counseling and decisional support. Findings support the need for interventional studies and enhanced focus on implementation for nurses to improve consumer-oriented outcomes.
ID1 and ID3 functions in the modulation of the tumour immune microenvironment in adult patients with B-cell acute lymphoblastic leukaemia
Authors: Poveda-Garavito Nathaly , Orozco Castaño Carlos A. , Torres-Llanos Yulieth , Cruz-Rodriguez Nataly , Parra-Medina Rafael , Quijano Sandra , Zabaleta Jovanny , Combita Alba Lucia 
Published: November 2024

IntroductionB-cell acute lymphoblastic leukemia (B-ALL) in adults often presents a poor prognosis. ID1 and ID3 genes have been identified as predictors of poor response in Colombian adult B-ALL patients, contributing to cancer development. In various cancer models, these genes have been associated with immune regulatory populations within the tumor immune microenvironment (TIME). B-ALL progression alters immune cell composition and the bone marrow (BM) microenvironment, impacting disease progression and therapy response. This study investigates the relationship between ID1 and ID3 expression, TIME dynamics, and immune evasion mechanisms in adult B-ALL patients. MethodsThis exploratory study analysed BM samples from 10 B-ALL adult patients diagnosed at the National Cancer Institute of Colombia. First, RT-qPCR was used to assess ID1 and ID3 expression in BM tumour cells. Flow cytometry characterised immune populations in the TIME. RNA-seq evaluated immune genes associatedwith B-ALL immune response, while xCell and CytoSig analysed TIME cell profiles and cytokines. Pathway analysis, gene ontology, and differential gene expression (DEGs) were examined, with functional enrichment analysis performed using KEGG ontology.ResultsPatients were divided into two groups based on ID1 and ID3 expression, namely basal and overexpression. A total of 94 differentially expressed genes were identified between these groups, with top overexpressed genes associated with neutrophil pathways. Gene set enrichment analysis revealed increased expression of genes associated with neutrophil degranulation, immune response-related neutrophil activation, and neutrophil-mediated immunity. These findings correlated with xCell data. Overexpression group showed significant differences in neutrophils, monocytes and CD4+ naive T cells compared to basal group patients. Microenvironment and immune scores were also significantly different, consistent with the flow cytometry results. Elevated cytokine levels associated with neutrophil activation supported these findings. Validation was performed using the Therapeutically Applicable Research to Generate Effective Treatments (TARGET) TCGA B-ALL cohorts.DiscussionThese findings highlight significant differences in ID1 and ID3 expression levels and their impact on TIME populations, particularly neutrophil-related pathways. The results suggest a potential role for ID1 and ID3 in immune evasion in adult B-ALL, mediated through neutrophil activation and immune regulation.
Long term efficacy and safety profile of dexamethasone intravitreal implant in retinal vein occlusions: a systematic review
Authors: Carnevali Adriano , Bacherini Daniela , Metrangolo Cristian , Chiosi Flavia , Viggiano Pasquale , Astarita Carlo , Gallinaro Valentina , Bonfiglio Vincenza M. E. 
Published: November 2024

Background/objectiveRetinal vein occlusion (RVO) is a common, sight-threatening vascular disorder affecting individuals of all ages, with incidence increasing with age. Due to its complex, multifactorial nature, treating RVO remains a clinical challenge. Currently, treatment strategies include laser photocoagulation (especially for branch RVO), anti-VEGF therapies, and intravitreal corticosteroids. This systematic review (without meta-analysis) aimed to update the evidence on the efficacy and safety of the sustained-release intravitreal dexamethasone implant (DEX-i) in managing macular edema (ME) secondary to central and branch RVO.MethodsA systematic review was conducted to assess current literature on DEX-i for ME secondary to RVO. Relevant studies were analyzed for outcomes related to visual acuity, retinal thickness, and the safety profile of DEX-i in RVO treatment.ResultsEvidence indicates that DEX-i substantially improves best-corrected visual acuity (BCVA) and reduces central retinal thickness (CRT) in ME associated with both branch and central RVO, demonstrating rapid and sustained effects. Common adverse events associated with DEX-i included manageable complications, such as medically controlled intraocular pressure elevation and progression of cataracts.ConclusionDEX-i offers effective and sustained improvements in both visual and anatomical outcomes for patients with ME secondary to RVO. Individualized treatment selection is essential to optimize patient outcomes. Future directions include identifying predictive biomarkers and adopting patient-centered approaches based on individual clinical characteristics, which may enhance treatment success in RVO.
Radioimmunotherapy combating biofilm-associated infection in vitro
Authors: Ye Zijian , van der Wildt Berend , Nurmohamed F. Ruben H. A. , van Duyvenbode J. Fred F. Hooning , van Strijp Jos , Vogely H. Charles , Lam Marnix G. E. H. , Dadachova Ekaterina , Weinans Harrie , van der Wal Bart C. H. , Poot Alex J. 
Published: November 2024

BackgroundAddressing prosthetic joint infections poses a significant challenge within orthopedic surgery, marked by elevated morbidity and mortality rates. The presence of biofilms and infections attributed to Staphylococcus aureus (S. aureus) further complicates the scenario.ObjectiveTo investigate the potential of radioimmunotherapy as an innovative intervention to tackle biofilm-associated infections.MethodsOur methodology involved employing specific monoclonal antibodies 4497-IgG1, designed for targeting wall teichoic acids found on S. aureus and its biofilm. These antibodies were linked with radionuclides actinium-225 (225Ac) and lutetium-177 (177Lu) using DOTA as a chelator. Following this, we evaluated the susceptibility of S. aureus and its biofilm to radioimmunotherapy in vitro, assessing bacterial viability and metabolic activity via colony-forming unit enumeration and xylenol tetrazolium assays.ResultsBoth [225Ac]4497-IgG1 and [177Lu]4497-IgG1 exhibited a noteworthy dose-dependent reduction in S. aureus in planktonic cultures and biofilms over a 96-h exposure period, compared to non-specific antibody control groups. Specifically, doses of 7.4 kBq and 7.4 MBq of [225Ac]4497-IgG1 and [177Lu]4497-IgG1 resulted in a four-log reduction in planktonic bacterial counts. Within biofilms, 14.8 kBq of [225Ac]4497-IgG1 and 14.8 Mbq [177Lu]4497-IgG1 led to reductions of two and four logs, respectively.ConclusionOur findings underscore the effectiveness of [225Ac]4497-IgG1 and [177Lu]4497-IgG1 antibodies in exerting dose-dependent bactericidal effects against planktonic S. aureus and biofilms in vitro. This suggests that radioimmunotherapy might serve as a promising targeted treatment approach for combating S. aureus and its biofilm.
Investigating the causal relationship between inflammation and multiple types of hearing loss: a multi-omics approach combining Mendelian randomization and molecular docking
Authors: Zhang Jingqi , Guo Tao , Chen Yaxin , Wang Xiangjin , Wu Lijiao , Xie Hui 
Published: November 2024

BackgroundHearing loss affects over 10% of the global population. Inflammation is a key factor in hearing loss caused by noise, infection, and aging, damaging various hearing-related tissues (e.g., spiral ligament, stria vascularis). Mendelian randomization (MR) can help identify potential causal relationships and therapeutic targets.MethodsWe conducted MR analyses on 91 inflammatory proteins (n = 14,824) and genome-wide association study results for various hearing loss types in European ancestry populations, including sensorineural hearing loss (SNHL; ncases = 15,952, ncontrols = 196,592), sudden idiopathic hearing loss (SIHL; ncases = 1,491, ncontrols = 196,592), and other hearing loss (OHL; ncases = 4,157, ncontrols = 196,592). Additionally, hearing loss with difficulty in hearing (ncases = 14,654, ncontrols = 474,839) served as a validation set. To predict inflammatory protein-enriched pathways and tissues, we performed enrichment analysis, functional annotation, and tissue analyses using “OmicsNet2.0” and “FUMA” platforms. We also combined “CoreMine” and molecular docking to explore potential drugs targeting inflammatory proteins and investigate binding efficacy.ResultsCCL19 was identified as a common risk factor for SNHL and OHL, which was validated in the hearing loss with difficulty in hearing dataset. Tissue analysis revealed that SIHL-related inflammatory proteins were enriched in the amygdala. Multi-omics research indicated associations between inflammatory proteins and neurodegenerative diseases. Molecular docking studies suggested that Chuanxiong Rhizoma and Uncariae Ramulus Cumuncis are potential drugs for targeting CCL19.ConclusionThis study identified CCL19 as a common risk factor for various types of hearing loss through MR analysis, highlighting the crucial role of inflammatory proteins in hearing loss. The enrichment of related inflammatory proteins in the amygdala and their association with neurodegenerative diseases provide new insights into the mechanisms of hearing loss.
Study on the correlation between energy availability and subclinical menstrual disorders
Authors: Bingzheng Zhou , Zhuo Jin , Qihao Wang , Lunhao Bai 
Published: November 2024

BackgroundThere are few studies on the correlation between energy availability (EA) and subclinical menstrual disorders (SMD) in female athletes. This study aims to explore the differences in EA between female athletes with eumenorrhea and those with SMD, and the correlation between EA and the occurrence of SMD.MethodsLuteal phase defect (LPD) and anovulation were defined as SMD. Fifty-six adult female college athletes with regular menstrual cycles and no clinical symptoms of menstrual disorders (MD) were selected as subjects. The EA of the subjects was monitored, and SMD were identified. The EA was compared between the two groups, and the correlation between EA and the occurrence of SMD was analyzed.ResultsNineteen subjects were identified as LPD, and 7 subjects were identified as anovulation. The occurrence of SMD was 46.4%. The EA of subjects with SMD was lower than that of subjects with eumenorrhea (t = 3.165, p = 0.003), and EA was negatively correlated with the occurrence of SMD (r = −0.396, p = 0.000).ConclusionThere were differences in EA between female college athletes with eumenorrhea and SMD. EA was negatively correlated with the occurrence of SMD.
Establishment and validation of a nomogram model for early diagnosis of gastric cancer: a large-scale cohort study
Authors: Wang Haiyu , Ding Yumin , Zhao Shujing , Li Kaixu , Li Dehong 
Published: November 2024

PurposeIdentifying high-risk populations and diagnosing gastric cancer (GC) early remains challenging. This study aimed to establish and verify a nomogram model for the early diagnosis of GC based on conventional laboratory indicators.MethodsWe performed a retrospective analysis of the clinical data of 2,770 individuals with first diagnosis of GC and 1,513 patients with benign gastric disease from January 2018 to December 2022. The cases were divided into the training set and validation set randomly, with a ratio of 7:3. Variable screening was performed by least absolute shrinkage and selection operator (LASSO) and logistic regression analysis. A nomogram was constructed in the training set to assist in the early diagnosis of GC.ResultsThere were 4283 patients included in the study, with 2998 patients assigned in the training set and 1285 patients in the validation set. Through LASSO regression and logistic regression analysis, independent variables associated with GC were identified, including CEA, CA199, LYM, HGB, MCH, MCHC, PLT, ALB, TG, HDL, and AFR. The nomogram model was constructed using the above 11 independent indicators. The AUC was 0.803 for the training set and 0.797 for the validation set, indicating that the model showed high clinical diagnostic efficacy. The calibration curves and decision curve analysis (DCA) of the nomogram presented good calibration and clinical application ability.ConclusionBased on the analysis of large sample size, we constructed a nomogram model with 11 routine laboratory indicators, which showed good discrimination ability and calibration.
Circular RNA CDR1as/ciRS-7– a novel biomarker in solid tumors
Authors: Zhang Yun , Xiong Chanyu , Jiang Zhilin , Wang Xiao , Ji Juanjuan , Pan Yan , Yu Tianshu , Wang Zihao , Zhu Lin , Yue Yumei , Li Qiong , Wang Haizhen , Zhu Shikai , Zhou Yu 
Published: November 2024

IntroductionCircular RNA CDR1as/ciRS-7 has been reported to function as an oncogenic regulator in various cancers. However, the prognostic value of CDR1as/ciRS-7 expression in solid tumors remains unclear. Herein, we conducted an updated meta-analysis to investigate the association between CDR1as/ciRS-7 expression and clinical outcomes in solid tumors.MethodsA systematic search was performed through the PubMed, EMBASE, Web of Science, and Ovid databases for eligible studies on clinical values of CDR1as/ciRS-7 in solid tumors. The pooled hazard ratios (HRs) or odd ratios (ORs) with 95% confidence intervals (CIs) were used to evaluate the correlation between CDR1as/ciRS-7 and clinical outcomes.ResultsA total of 2424 patients from 17 studies between 2017 and 2023 were included. The results suggested that elevated CDR1as/ciRS-7 expression predicted a poor overall survival (OS) for 12 types of solid tumors (HR=1.93, 95% CI: 1.43-2.60, P&lt;0.001) with no heterogeneity (I2 = 80.2%, P&lt;0.001). Stratified analysis indicated that there was a negative relationship between CDR1as/ciRS-7 expression and OS in digestive system cancers (HR=2.30, 95% CI: 1.84-2.88, P&lt;0.001), and respiratory cancers (HR=2.40, 95% CI: 1.75-3.30, P&lt;0.001). Furthermore, we also revealed that CDR1as/ciRS-7 was positively related to tumor size (OR=2.11, 95%CI: 1.64-2.71, P&lt;0.001), TNM stage (OR=2.05, 95%CI: 1.65-2.54, P&lt;0.001), lymph node metastasis (LNM) (OR=1.74, 95%CI: 1.38-2.21, P&lt;0.001), and distant metastasis (OR=2.79, 95%CI: 1.71-4.55, P&lt;0.001). Although the probable evidence of publication bias was found in the studies with OS, tumor size, TNM stage, and LNM, the trim and fill analysis confirmed the reliability of these results was not affected.ConclusionElevated CDR1as/ciRS-7 expression was associated with larger tumor size, advanced TNM stage, worse LNM, distant metastasis, and shorter OS, suggesting that CDR1as/ciRS-7 may act as an independent prognostic biomarker in solid tumors.