bluuebunny/crossref_metadata_2025_split huggingface dataset:
Metabolic modulations of Pseudomonas graminis in response to H2O2 in cloud water
Authors: Wirgot Nolwenn , Lagrée Marie , Traïkia Mounir , Besaury Ludovic , Amato Pierre , Canet Isabelle , Sancelme Martine , Jousse Cyril , Diémé Binta , Lyan Bernard , Delort Anne-Marie 
Published: September 2019

AbstractIn cloud water, microorganisms are exposed to very strong stresses especially related to the presence of reactive oxygen species including H2O2 and radicals, which are the driving force of cloud chemistry. In order to understand how the bacterium Pseudomonas graminis isolated from cloud water respond to this oxidative stress, it was incubated in microcosms containing a synthetic solution of cloud water in the presence or in the absence of H2O2. P. graminis metabolome was examined by LC-MS and NMR after 50 min and after 24 hours of incubation. After 50 min, the cells were metabolizing H2O2 while this compound was still present in the medium, and it was completely biodegraded after 24 hours. Cells exposed to H2O2 had a distinct metabolome as compared to unexposed cells, revealing modulations of certain metabolic pathways in response to oxidative stress. These data indicated that the regulations observed mainly involved carbohydrate, glutathione, energy, lipid, peptides and amino-acids metabolisms. When cells had detoxified H2O2 from the medium, their metabolome was not distinguishable anymore from unexposed cells, highlighting the capacity of resilience of this bacterium. This work illustrates the interactions existing between the cloud microbial metabolome and cloud chemistry.
Active behaviour during early development shapes glucocorticoid reactivity
Authors: Castillo-Ramírez Luis A. , Ryu Soojin , De Marco Rodrigo J. 
Published: September 2019

AbstractGlucocorticoids are the final effectors of the stress axis, with numerous targets in the central nervous system and the periphery. They are essential for adaptation, yet currently it is unclear how early life events program the glucocorticoid response to stress. Here we provide evidence that involuntary swimming at early developmental stages can reconfigure the cortisol response to homotypic and heterotypic stress in larval zebrafish (Danio rerio), also reducing startle reactivity and increasing spontaneous activity as well as energy efficiency during active behaviour. Collectively, these data identify a role of the genetically malleable zebrafish for linking early life stress with glucocorticoid function in later life.
Near-field acoustic manipulation in a confined evanescent Bessel beam
Authors: Gires Pierre-Yves , Poulain Cédric 
Published: September 2019

AbstractWe demonstrate the potential of using evanescent fields, instead of conventional propagating sound fields, to manipulate particles at micro or nano scale. We generate an evanescent acoustic Bessel beam in liquid above a thin, circular, asymmetrically excited plate. In the sub-MHz ultrasound domain, the resulting radiation force causes the particles to assemble at the pressure antinodes along concentric circles corresponding to the Bessel profile. By imposing an axial confinement in the evanescent region, the subwavelength two-plate sandwich system becomes resonant, increasing the radiation force magnitude. Resonances occur for some well-defined gaps for which whole numbers of antinodal circles are observed. Through fine tuning, particles as small as bacteria can be patterned. Further amplification can be obtained by trapping a microbubble in the Bessel beam axis. As we show, this resonant bubble, which acts as an acoustic magnet, can be used to efficiently capture or repel nearby micro-particles.
The sound of Bell states
Authors: Hasan M. Arif , Calderin Lazaro , Lata Trevor , Lucas Pierre , Runge Keith , Deymier Pierre A. 
Published: September 2019

AbstractNonseparable states, analogous to “entangled” states, have generated great scientific interest since the very beginning of quantum mechanics. To date, however, the concept of “classical nonseparability” has only been applied to nonseparable states of different degrees-of-freedom in laser beams. Here, we experimentally demonstrate the preparation and tunability of acoustic nonseparable states, i.e. Bell states, supported by coupled elastic waveguides. A Bell state is constructed as a superposition of elastic waves, each a tensor product of a spinor part and an orbital angular momentum (OAM) part, which cannot be factored as a single tensor product. We also find that the amplitude coefficients of the nonseparable superposition of states must be complex. By tuning these complex amplitudes, we are able to experimentally navigate a sizeable portion of the Bell state’s Hilbert space. The current experimental findings open the door to the extension of classical nonseparability to the emerging field of phononics.
Understanding User Reactions and Interactions With an Internet-Based Intervention for Tinnitus Self-Management: Mixed-Methods Evaluation
Authors: Greenwell Kate , Sereda Magdalena , Coulson Neil S. , Hoare Derek J. 
Published: September 2019

Purpose
Internet-based interventions have the potential to reduce the disparity in access to psychological therapy that people with tinnitus currently experience. One example is the Tinnitus E-Programme, which, although freely available online, has not yet been formally evaluated. The purpose of this study was to evaluate past, current, and new users' reactions and interactions with the Tinnitus E-Programme.

Method

Study 1 used an online survey to gather past and current users' reactions to, and interactions with, the intervention (
n
= 27). Study 2 used interviews and a relaxation log to assess how new users implemented the skills they learned into their everyday lives (
n
= 13).

Results
Generally, users expressed positive views on the intervention content and design features. Users particularly valued the education about tinnitus and its management and the relaxation skills training, and use of these components was high. In contrast, user reactions to self-monitoring tools, an online support forum, and therapist support were mixed, and use was lower. Implementation was limited by instances of poor usability and accessibility, user engagement, and adherence to relaxation goals. Users' perceptions of the intervention's credibility and relevance and beliefs regarding a negative impact on their tinnitus influenced engagement. Users in both studies identified several benefits gained, including functional and emotional management, self-efficacy for managing and coping with tinnitus, understanding tinnitus and its management, social support, and acceptance of tinnitus.

Conclusion
Findings suggest that the intervention was acceptable to its target group but also highlighted some areas for improvement. These findings will be used to inform further optimization work.
Miniaturised preparation of polymeric nanoparticles using droplet manipulation on open surfaces
Authors: Abualsayed Alsaeed M. , Abouelmagd Sara A. , Abdelgawad Mohamed 
Published: September 2019

A digital microfluidics platform for the preparation of poly(lactic‐co‐glycolic) acid (PLGA) nanoparticles (NPs) was developed. Droplets of PLGA in dimethylformamide were merged with droplets of deionised water by electrical actuation on a digital microfluidics device to form PLGA NPs through nanoprecipitation. The developed platform is automated and allows for the preparation of polymeric NPs with small size and high uniformity. Using the platform, the authors were able to prepare monodisperse PLGA NPs as small as 115 nm with a polydispersity index (PDI) of 0.14 which can be challenging with conventional preparation techniques on the macroscale. Size of the prepared NPs can be tuned through proper choice of the volume ratio between the two merged droplets which controls the induced internal convection flow after merging. Concentration of PLGA in the dimethylformamide droplet also had an effect on the size and polydispersity of the formed NPs. These results prove the potential use of digital microfluidics for testing combinatorial synthesis of different polymeric NPs for various applications. This approach allows robust and automated screening of NP preparations using only few microlitres of the reagents used, thus conserving precious and costly NP components and loaded therapeutic agents.
Jammern und Klagen: Stressfaktor Nummer
eins
Authors: Jürgens Ute 
Published: September 2019

ZusammenfassungDer Kollege hat an allem etwas auszusetzen – egal, um wen oder was es geht. Die
Patienten sind unfreundlich, die Chefin kümmert sich um nichts, die Kollegin ist
zu langsam und der Praktikant schreibt die Protokolle zu umständlich. Manche
Menschen sind vom Pessimismus regelrecht befallen und nörgeln den ganzen Tag.
Leider ist eine Nörgelhaltung bei Personen im eigenen Umfeld ansteckend.
Adulte Aufmerksamkeitsdefizit-/Hyperaktivitätsstörungen und Persönlichkeitsstörungen
Authors: Jacob Christian 
Published: September 2019

ZUSAMMENFASSUNGBetroffene von adulte Aufmerksamkeitsdefizit-/Hyperaktivitätsstörungen (aADHS) weisen hohe Komorbidität mit Persönlichkeitsstörungen aus allen 3 Clustern auf, dem eigentümlich-exzentrischen Cluster A, dem emotional-launischen Cluster B und dem ängstlich-furchtsamen Cluster C. Die Komorbidität von aADHS und Persönlichkeitsstörungen ist sowohl in klinischen als auch in nicht klinischen Stichproben deutlich erhöht. Inkonsistent sind die Befunde, welche der Cluster B Persönlichkeitsstörungen dissoziale, Borderline, histrionische oder narzisstische Persönlichkeitsstörungen die höchste Komorbidität aufweisen. Die Komorbiditätsbeziehungen werden kontrovers diskutiert. Es gibt Hinweise auf eine hohe psychosoziale Relevanz der Komorbidität von aADHS mit Persönlichkeitsstörungen.
Wie geht’s weiter, wenn wir an unsere Grenzen
kommen?
Authors: Mark Alexander , Heinicke Tobias 
Published: September 2019

ZusammenfassungDie Arbeit von Intensivpflegenden ist mit vielfältigen Belastungen verknüpft.
Regelmäßige Besprechungen im multidisziplinären Team können helfen, mit
schwierigen Situationen umzugehen. Ein Team der Uniklinik Erlangen hat dazu ein
Ampel-System eingeführt, mit dessen Hilfe jeder Mitarbeiter schwierige
Situationen benennen kann, die in der Folge nach Dringlichkeit abgestuft,
systematisch und verbindlich im Team besprochen werden.
Empfehlungen zur systemischen Therapie des metastasierten Nierenzellkarzinoms 2019
Authors: Kröger Nils , Merseburger Axel S. , Bedke Jens 
Published: September 2019

ZusammenfassungFür die Behandlung des metastasierten Nierenzellkarzinoms steht eine Vielzahl an Medikamenten für die Systemtherapie zur Verfügung. Diese werden sowohl in der Erst- als auch Zweitlinientherapie und in späteren Linien eingesetzt. Die vorliegende Übersichtsarbeit beschreibt die in Deutschland zugelassenen Medikamente der Tyrosinkinase-, mTOR- und Immuncheckpointinhibition für die Systemtherapie des metastasierten klarzelligen Nierenzellkarzinoms und gibt eine Empfehlung für deren klinischen Einsatz aufgrund der vorhanden Phase II- und Phase III-Studiendaten. Des Weiteren werden detailliert die potenziellen Nebenwirkungen der einzelnen Substanzen beschrieben.
Geschlecht: Die Magie der Anisogamie
Authors: Villa Paula-Irene 
Published: September 2019

ZusammenfassungDer Beitrag setzt sich mit den Argumenten und Studien auseinander, die Ponseti
und Stirn aufbringen. Ihnen wird ein biologischer Reduktionismus bescheinigt,
der paradoxerweise das Unbehagen an der identitäts-subjektiven Engführung von
Geschlecht durch eine spiegelbildliche Engführung von Geschlecht auf Anisogamie
wiederholt. Als bessere Alternative wird ein biosoziales Verständnis von
Geschlecht skizziert, das der empirischen Komplexität von Geschlechtlichkeit
sowie dessen (Un-)verfügbarkeit besser gerecht wird.
Schwierigkeiten bei der Interpretation von
Längsschnittstudien bei Menschen mit Geschlechtsinkongruenz oder
Geschlechtsdysphorie: Ein Kommentar zu Ponseti und Stirn (2019)
Authors: Strauß Bernhard , Nieder Timo O. 
Published: September 2019

ZusammenfassungDer Kommentar nimmt Bezug auf die von Ponseti und
Stirn (2019) formulierte Kritik an der S3-Leitlinie zur Diagnostik,
Beratung und Behandlung bei Geschlechtsinkongruenz, Geschlechtsdysphorie und
Trans-Gesundheit. Dabei problematisieren wir zunächst die begriffliche
Gleichsetzung von Transsexualismus auf der einen mit Geschlechtsinkongruenz und
Geschlechtsdysphorie auf der anderen Seite. Im Anschluss diskutieren wir die
unzulässigen Schlüsse, die Ponseti und Stirn aus den Befunden einzelner
Katamnesestudien ziehen und argumentieren, dass die genannten Befunde keine
Rückschlüsse auf die Wirksamkeit der Behandlung des Transsexualismus zulassen,
sondern ausschließlich die Gesundheit von trans Personen nach multimodaler
geschlechtsangleichender Behandlung beschreiben. Schließlich stellen wir die
Überlegungen infrage, die die Autor_innen zur Psychotherapie des
Transsexualismus in ihrem Beitrag äußern.
A case of bulky pseudohyperplastic prostatic adenocarcinoma
Authors: Narita Tomoya , Kondo Atsuya , Maeda Motohiro , Funahashi Yasuhito , Tanaka Kuniaki , Gotoh Momokazu 
Published: September 2019

AbstractA 67-year-old male with a pelvic mass 13 × 7 cm in dimension was diagnosed with a pseudohyperplastic prostatic adenocarcinoma via mass biopsy. Androgen-deprivation therapy was remarkably effective, resulting in rapid tumor shrinkage.
Of Cestodes and Men: Surgical Treatment of a Spinal Hydatid Cyst
Authors: Saul Dominik , Seitz Mark-Tilmann , Weiser Lukas , Oberthür Swantje , Roch Jonathan , Bremmer Felix , Perske Christina , Viezens Lennart , Sehmisch Stephan , Lehmann Wolfgang 
Published: September 2019

Abstract
Background The cestode Echinococcus granulosus causes hydatid disease. In addition to manifestations in the liver and lung, it can lead to cystic lesions in the spine.
Case Description We report a 42-year-old male patient with primary hydatid disease in the eighth thoracic vertebra. The only clinical symptom was chronic back pain. Although laboratory findings were normal, imaging displayed lytic destruction that raised the suspicion of a metastatic disease. Diagnostics of the thoraces and abdomen did not reveal other pathologic abnormalities. Follow-up magnetic resonance imaging (MRI) depicted a progressive compression of the spinal cord and inhomogeneous structure in the fat-suppressed sequences. Because the Jamshidi biopsy was inconclusive, the tumor board recommended surgery. Dorsal decompression, spondylodesis of T6–T10, and vertebral column resection of T8 with complete cyst removal were performed. The resected vertebrae showed a mucous-like lesion with white granular tissue interfusing the whole vertebral body. A pathologic examination and enzyme-linked immunosorbent assay confirmed E. granulosus. Thus chemotherapy with albendazole was initiated. A follow-up MRI of the whole spine confirmed complete remission and found no additional resettlements. The patient's back pain was resolved without neurologic deficits.
Conclusions For lytic manifestations of the vertebral column, hydatid cysts should be considered a differential diagnosis in addition to malignant metastasis, tuberculosis, and osteomyelitis. Thorough surgical resection and strict follow-up are necessary.
Sub-Bandage Pressure in the Canine Forelimb after Rigid Splint Application by Surgeons and Veterinary Students
Authors: Vitt Molly A. , Wingert Daniel C. , Conzemius Michael G. 
Published: September 2019

Objective The aim of this study was to measure and compare sub-bandage pressures after a rigid splint was applied to the forelimb of a dog by surgeons and veterinary students.
Animals One, adult, Labrador Retriever.
Methods Sub-bandage pressure was measured at five locations on the limb of a dog using a previously validated pneumatic compression measurement system over a 4-hour period after splint application. All participants received the same instructions and the same dog was used for each splint application.
Results Across time and location, mean sub-bandage pressures from the experienced group were significantly greater than those from the inexperienced group at all transducer locations and at all time points. People from the inexperienced group recorded the greatest range in sub-bandage pressures and had significantly higher-pressure differences across the five locations sub-bandage pressure was measured.
Conclusions and Clinical Relevance Surgeons applied their splint bandages with approximately 50% greater pressure and 50% less variability between locations. The large range in sub-bandage pressures found may suggest that decreased and/or increased sub-bandage pressure may predispose to bandage complications.
Children with Hirschsprung's Disease and Syndromes with Cognitive Dysfunction: Manifestations, Treatment, and Outcomes
Authors: Hedbys Josefine , Hasserius Johan , Granéli Christina , Arnbjörnsson Einar , Hagelsteen Kristine , Stenström Pernilla 
Published: September 2019

Abstract
Introduction To assess differences in initial symptoms, treatments, and bowel function between children with Hirschsprung's disease (HD) with or without a cognitive dysfunction (CD).
Materials and Methods The study included children with HD who underwent transanal endorectal pull-through. A retrospective chart review was performed to collect data on patient characteristics, diagnosis, and treatment. Data on bowel symptoms in children older than 4 years without a colostomy were compiled during a cross-sectional, patient-reported follow-up.
Results Fifty-three children with HD were included; of these, 12 (23%) had CD. The median birth weight was lower, frequency of vomiting as the presenting symptom was lower, and time until the first contact with a pediatric surgeon was higher in children with CD than in those without (3,295 vs. 3,623 g, p = 0.013; 28 vs. 66%, p = 0.02; and 4 days vs. 1 day, p = 0.048, respectively). At follow-up, 5 (15%) of 33 children aged over 4 years had CD. More children without CD had some ability to hold back defecation and sense the urge to defecate than those with CD (p = 0.002 and p = 0.001, respectively).
Conclusion HD children who have CD present with different initial symptoms, have a delay in the first consultation with a pediatric surgeon, and experience poorer bowel function outcomes than HD children without CD. Therefore, HD children with CD should receive special attention in both clinical practice and research.
Wound Infection after Laparoscopic-Assisted Gastrostomy in Infants
Authors: Burman Linnéa , Diaz Maia , Viktorsdóttir Margrét Brands , Sjövie Helen , Stenström Pernilla , Salö Martin , Arnbjörnsson Einar Ólafur 
Published: September 2019

Abstract
Background Gastrostomy placement in children is one of the most frequently performed pediatric surgical procedures and laparoscopic-assisted gastrostomy (LAG) is the preferred technique. Wound infection after LAG has become a significant concern due to the emergence of antibiotic resistance. The aim of this study was to describe the frequency of wound infection after LAG in children younger than 2 years of age and to identify the associated risk factors and the bacterial species involved.
Methods Information about wound infection, results from bacterial cultures, and type of antibiotic treatment used within 30 postoperative days after LAG were compiled for infants who underwent LAG from 2010 to 2017. A retrospective chart review was performed. Data was compiled from charts and from an electronic database containing prospectively collected data. A multivariate logistic analysis was used to explore potential risk factors. Preoperative antibiotic prophylaxis and postoperative local wound care were conducted according to standard procedures.
Results The 141 included infants underwent surgery at a median age of 10 months (range: 1–24). Thirty-eight (27%) patients had a clinically determined wound infection, bacteria were cultured from 26/38 (69%), and 30/38 (79%) received antibiotic treatment. The median interval from surgery to detection of a clinical wound infection was 14 days (range: 4–30). The most common microbes discovered were skin bacteria Staphylococcus aureus or Streptococcus pyogenes, but respiratory and intestinal bacteria were also found. Multivariate logistic regression analysis revealed no independent risk factors for infection such as age, gender, or underlying diagnosis.
Conclusion Infants have a high rate of postoperative clinical wound infection after LAG despite the use of preoperative antibiotic prophylaxis and intense local wound care. Gender, age at operation, and previous diagnoses were not found to be independent risk factors for wound infection.
Expression analysis of genes encoding malectin-like domain (MLD)- and leucine-rich repeat (LRR)- containing proteins in Arabidopsis thaliana
Authors: Sultana Mst Momtaz , Hachiya Takushi , Dutta Amit Kumar , Nishimura Kohji , Suzuki Takamasa , Tanaka Ai , Nakagawa Tsuyoshi 
Published: September 2019

ABSTRACT
Malectin is a maltose-binding endoplasmic reticulum protein conserved in animals. In Arabidopsis thaliana, we identified four genes that encode malectin-like domain (MLD)- and leucine-rich repeat (LRR)-containing proteins (AtMLLRs): two were receptor-like proteins (AtMLLR1 and 2) and the other two were extracellular proteins (AtMLLR3 and 4). The promoter:G3GFP+promoter:GUS assay indicated the organ- and cell-specific expression of the AtMLLR2 and AtMLLR3 genes.
Abbreviations: Cmr: chloramphenicol-resistance marker; G3GFP: G3 green fluorescent protein; GUS: β-glucuronidase; KD: kinase domain; LRR: leucine-rich repeat; MLD: malectin-like domain; RLK: receptor-like kinase; SP: signal peptide; TMD: transmembrane domain; Tnos: nopaline synthase terminator
Common source of light emission and nonlocal molecular manipulation on the Si(111)−7 × 7 surface
Authors: Purkiss Rebecca M , Etheridge Henry G , Sloan Peter A , Rusimova Kristina R 
Published: September 2019

Abstract
The tip of a scanning tunnelling microscope can inject hot electrons into a surface with atomic precision. Their subsequent dynamics and eventual decay can result in atomic manipulation of an adsorbed molecule, or in light emission from the surface. Here, we combine the results of these two near identical experimental techniques for the system of toluene molecules chemisorbed on the Si(111)−7 × 7 surface at room temperature. The radial dependence of molecular desorption away from the tip injection site conforms to a two-step ballistic-diffusive transport of the injected hot electrons across the surface, with a threshold bias voltage of +2.0 V. We find the same threshold voltage of +2.0 V for light emission from the bare Si(111)−7 × 7 surface. Comparing these results with previous published spectra we propose that both the manipulation (here, desorption or diffusion) and the light emission follow the same hot electron dynamics, only differing in the outcome of the final relaxation step which may result in either molecular displacement, or photon emission.
Aspherical and covalent bonding character of d electrons of molybdenum from synchrotron x-ray diffraction
Authors: Sasaki Tomoaki , Kasai Hidetaka , Nishibori Eiji 
Published: September 2019

Abstract
The occupancies and spatial distribution of electrons for 4d-orbitals in pure molybdenum have been experimentally determined by a charge density study from synchrotron radiation x-ray powder diffraction. There are valence charge density maxima in interatomic positions indicating bond formation. The electron deficiencies of Γ12 orbitals were visualized in the observed static deformation density. An electron deficiency of ∼0.5 was observed from the orbital population analysis through multipole refinement. The occupancies and spatial distribution have also been calculated by a density functional theoretical calculation using WIEN2k packages for comparison. The observed features agree well with the theoretical study. In addition, the observed charge density has more covalent bonding character than the theoretical one. The present study confirms that a state-of-the-art x-ray charge density study can reveal the spatial structure of d-electrons in 4d-system.