bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impaired airway mucociliary function reduces antigen-specific IgA immune response to immunization with a claudin-4-targeting nasal vaccine in mice
Authors: Suzuki Hidehiko , Nagatake Takahiro , Nasu Ayaka , Lan Huangwenxian , Ikegami Koji , Setou Mitsutoshi , Hamazaki Yoko , Kiyono Hiroshi , Yagi Kiyohito , Kondoh Masuo , Kunisawa Jun 
Published: February 2018

AbstractVaccine delivery is an essential element for the development of mucosal vaccine, but it remains to be investigated how physical barriers such as mucus and cilia affect vaccine delivery efficacy. Previously, we reported that C-terminal fragment ofClostridium perfringensenterotoxin (C-CPE) targeted claudin-4, which is expressed by the epithelium associated with nasopharynx-associated lymphoid tissue (NALT), and could be effective as a nasal vaccine delivery. Mice lacking tubulin tyrosine ligase-like family, member 1 (Ttll1-KO mice) showed mucus accumulation in nasal cavity due to the impaired motility of respiratory cilia.Ttll1-KO mice nasally immunized with C-CPE fused to pneumococcal surface protein A (PspA-C-CPE) showed reduced PspA-specific nasal IgA responses, impaired germinal center formation, and decreased germinal center B-cells and follicular helper T cells in the NALT. Although there was no change in the expression of claudin-4 in the NALT epithelium inTtll1-KO mice, the epithelium was covered by a dense mucus that prevented the binding of PspA-C-CPE to NALT. However, administration of expectorant N-acetylcysteine removed the mucus and rescued the PspA-specific nasal IgA response. These results show that the accumulation of mucus caused by impaired respiratory cilia function is an interfering factor in the C-CPE-based claudin-4-targeting nasal vaccine.
Hydrophilic fluorinated molecules for spectral 19F MRI
Authors: Tanifum Eric A. , Patel Chandreshkumar , Liaw Matthew E. , Pautler Robia G. , Annapragada Ananth V. 
Published: February 2018

AbstractFluorine-19 (19F) Magnetic Resonance Imaging (MRI) is an emerging modality for molecular imaging and cell tracking. The hydrophobicity of current exogenous probes, perfluorocarbons (PFCs) and perfluoropolyethers (PFPEs), limits the formulation options available for in vivo applications. Hydrophilic probes permit more formulation flexibility. Further, the broad Nuclear Magnetic Resonance (NMR) chemical shift range of organofluorine species enables multiple probes with unique 19F MR signatures for simultaneous interrogation of distinct molecular targets in vivo. We report herein a flexible approach to stable liposomal formulations of hydrophilic fluorinated molecules (each bearing numerous magnetically equivalent 19F atoms), with 19F encapsulation of up to 22.7 mg/mL and a per particle load of 3.6 × 106 19F atoms. Using a combination of such probes, we demonstrate, with no chemical shift artifacts, the simultaneous imaging of multiple targets within a given target volume by spectral 19F MRI.
Unbalanced inflammatory reaction could increase tissue destruction and worsen skin infectious diseases – a comparative study of leishmaniasis and sporotrichosis
Authors: Morgado F. N. , de Carvalho L. M. V. , Leite-Silva J. , Seba A. J. , Pimentel M. I. F. , Fagundes A. , Madeira M. F. , Lyra M. R. , Oliveira M. M. , Schubach A. O. , Conceição-Silva F. 
Published: February 2018

AbstractThe clinical presentations of skin diseases produced by different pathogens, as American tegumentary leishmaniasis (ATL) and sporotrichosis can be similar and possibly influenced by the skin immune system (SIS). The aim of the study was to understand the underlying mechanisms of skin inflammation produced by different pathogens. We used immunohistochemistry to analyze 96 patients: a- localized cutaneous leishmaniasis (LCL-ATL); b- sporotrichoid cutaneous leishmaniasis (SCL-ATL); c-lymphocutaneous (LC-SP); d- fixed (F-SP) sporotrichosis. LCL-ATL and SCL-ATL had a significantly higher percentage of CD8, FasL and NOS2 than sporotrichosis. In contrast, LC-SP had a substantially higher percentage of CD4, BCl2 and neutrophils than ATL lesions. These results indicated some differences in the profile of thein situimmune response suggesting that SIS is a complex, adaptable system capable of different responses to intracellular or extracellular pathogens. However, regardless of the etiological agents, the inflammatory reaction and clinical manifestations can be similar. SCL-ATL and LC-SP presented similarities in both clinical presentation andin situinflammatory profile (CD3, CD22, neutrophils, macrophages). The clinical presentation of ATL and sporotrichosis could be explained by a combination of factors both of the host SIS and the etiological agent. The unbalanced host parasite relationship could result in atypical manifestations of skin disease.
Utterance Duration as It Relates to Communicative Variables in Infant Vocal Development
Authors: Ramsdell-Hudock Heather L. , Stuart Andrew , Parham Douglas F. 
Published: February 2018

Purpose
We aimed to provide novel information on utterance duration as it relates to vocal type, facial affect, gaze direction, and age in the prelinguistic/early linguistic infant.

Method
Infant utterances were analyzed from longitudinal recordings of 15 infants at 8, 10, 12, 14, and 16 months of age. Utterance durations were measured and coded for vocal type (i.e., squeal, growl, raspberry, vowel, cry, laugh), facial affect (i.e., positive, negative, neutral), and gaze direction (i.e., to person, to mirror, or not directed).

Results
Of the 18,236 utterances analyzed, durations were typically shortest at 14 months of age and longest at 16 months of age. Statistically significant changes were observed in utterance durations across age for all variables of interest.

Conclusion
Despite variation in duration of infant utterances, developmental patterns were observed. For these infants, utterance durations appear to become more consolidated later in development, after the 1st year of life. Indeed, 12 months is often noted as the typical age of onset for 1st words and might possibly be a point in time when utterance durations begin to show patterns across communicative variables.
Characterising open clusters in the solar neighbourhood with the Tycho-Gaia Astrometric Solution
Authors: Cantat-Gaudin T. , Vallenari A. , Sordo R. , Pensabene F. , Krone-Martins A. , Moitinho A. , Jordi C. , Casamiquela L. , Balaguer-Núnez L. , Soubiran C. , Brouillet N. 
Published: February 2018

Context. The Tycho-Gaia Astrometric Solution (TGAS) subset of the first Gaia catalogue contains an unprecedented sample of proper motions and parallaxes for two million stars brighter than G ~ 12 mag.
Aims. We take advantage of the full astrometric solution available for those stars to identify the members of known open clusters and compute mean cluster parameters using either TGAS or the fourth U.S. Naval Observatory CCD Astrograph Catalog (UCAC4) proper motions, and TGAS parallaxes.
Methods. We apply an unsupervised membership assignment procedure to select high probability cluster members, we use a Bayesian/Markov Chain Monte Carlo technique to fit stellar isochrones to the observed 2MASS JHKS magnitudes of the member stars and derive cluster parameters (age, metallicity, extinction, distance modulus), and we combine TGAS data with spectroscopic radial velocities to compute full Galactic orbits.
Results. We obtain mean astrometric parameters (proper motions and parallaxes) for 128 clusters closer than about 2 kpc, and cluster parameters from isochrone fitting for 26 of them located within a distance of 1 kpc from the Sun. We show the orbital parameters obtained from integrating 36 orbits in a Galactic potential.
Webb–Dattani Syndrome: Report of a Saudi Arabian Family with a Novel Homozygous Mutation in the ARNT2 Gene
Authors: Al-Sannaa Nouriya Abbas , Pepler Alexander , Al-Abdulwahed Hind Y. , Al-Majed Sami I. , Abdi Rifat F. , Menzel Moritz , Biskup Saskia 
Published: February 2018

AbstractWebb–Dattani syndrome (WEDAS) is an autosomal recessive disorder caused by mutation in the ARNT2 gene characterized by frontotemporal hypoplasia, globally delayed development, and pituitary and hypothalamic insufficiency. The condition is reported to be associated with consanguinity and with Saudi Arabian ancestry. Here we describe a family of three affected siblings born to healthy second cousin parents of Saudi Arabian ancestry. The children presented at 3 months of age with congenital central hypotonia and hypoventilation, central diabetes insipidus, multiple pituitary hormone deficiency, severe developmental delay, acquired microcephaly, cortical blindness with normal retinal examination, seizures, and gastroesophageal reflux. Whole exome sequencing detected a homozygous unclear variant (c.378C>T; p.G126G) in the ARNT2 gene in both the affected twins. According to splice prediction programs, this variant results in the creation of a cryptic donor splice site, possibly leading to a loss of function. These data support the role of the detected mutation in the ARNT2 gene in causing the described phenotype.
Adenoma recurrence after endoscopic mucosal resection: propensity score analysis of old and new colonoscopes and Sydney recurrence tool implementation
Authors: Vinsard Daniela , Kandel Pujan , Mejia Perez Lady , Bingham Russell , Lennon Ryan , Woodward Timothy , Gomez Victoria , Raimondo Massimo , Bouras Ernest , Wallace Michael 
Published: February 2018

Abstract
Background and study aims Risk factors for colorectal adenoma recurrence after endoscopic mucosal resection (EMR) have been well documented. We assessed the efficacy of the newer 190 colonoscope versus the standard 180 colonoscope for complete resection of lateral spreading lesions.
Patients and methods A single-center, retrospective study of patients who underwent EMR with Olympus 180 or 190 colonoscopes from January 1, 2010 to September 30, 2016. We included patients with lesions ≥ 20 mm and surveillance colonoscopy (SC1) after index EMR. A propensity score approach with inverse probability weighting was used to control for potential confounders. A secondary aim was to identify risk factors for recurrence and assess the applicability of the Sydney EMR recurrence tool (SERT) by grading each lesion of our cohort and analyzing associations with recurrence.
Results Two hundred ninety-one lesions met inclusion criteria for the study. Odds ratio (OR) for recurrence with the 190 colonoscope was 1.06 (P = .85). Adenoma size (P = .02) and use of argon plasma coagulation (APC; P < .001) were risk factors for recurrence. Lesions with SERT scores > 0 had a higher recurrence risk during follow-up (32 % vs 21 %; OR 1.71; P = .05). Lesions with SERT scores = 0 reached a plateau for recurrence at 12 and 18 months in Kaplan-Meier curves.
Conclusions The use of 190 colonoscopes did not measurably affect adenoma recurrence at SC1. Recurrence was associated with adenoma size, complementary APC for resection, and SERT scores > 0. Lesions with SERT scores = 0 that remain negative for recurrence at 18 months may return to routine surveillance.
Extended cold snare polypectomy for small colorectal polyps increases the R0 resection rate
Authors: Abe Yasuhiro , Nabeta Haruaki , Koyanagi Ryota , Nakamichi Taro , Hirashima Hayato , Lefor Alan , Shinozaki Satoshi 
Published: February 2018

Abstract
Background and study aims Despite widespread use of cold snare polypectomy (CSP), the R0 resection rate is not well documented. We perform extended CSP, resecting polyps with a > 1 mm circumferential margin. The aim of this study is to compare the R0 resection rate of extended CSP with conventional CSP and to assess safety.
Patients and methods From April 2014 to September 2016, 712 non-pedunculated colorectal polyps, < 10 mm in size, resected using CSP from 316 patients were retrospectively analyzed.
Results We divided lesions into conventional CSP (n = 263) and extended CSP groups (n = 449). The baseline characteristics of these two groups were not significantly different in univariate or multivariate analyses. Sessile polyps comprised 94 % (668/712), and the remaining were flat-elevated polyps. Mean size of polyps (±standard deviation) was 4.2 ± 1.5 mm. The most frequent pathology was low grade adenoma (97 %, 689/712). The R0 resection rate was significantly higher in the extended CSP group (439/449 [98 %]) than in the conventional CSP group (222/263 [84 %], P < 0.001). There was no delayed bleeding or perforation in either group (conventional CSP group, 0/263, 95 % confidence interval: 0.0 – 1.4 % and extended CSP group, 0/449, 95 % confidence interval: 0.0 – 0.8 %).
Conclusions Extended CSP results in a higher R0 resection rate compared with conventional CSP. Extended CSP did not result in a higher rate of delayed bleeding or perforation. Extended CSP is a safe and promising procedure for endoscopic resection of non-pedunculated colorectal polyps < 10 mm in size
Fetal Cardiac Remodeling in Response to Anemia: Using Hemoglobin Bart’s Disease as a Study Model
Authors: Tongsong Theera , Tongprasert Fuanglada , Srisupundit Kasemsri , Luewan Suchaya , Traisrisilp Kuntharee , Jatavan Phudit 
Published: February 2018

Abstract
Objective To assess fetal cardiac remodeling in response to anemia, by comparing the fetal cardiac dimensions and global sphericity index (GSI) of normal fetuses and fetuses with anemia using fetal Hb Bart’s disease as a study model.
Methods Fetuses at risk for Hb Bart’s disease undergoing cordocentesis at 18 to 22 weeks of gestation were recruited. Fetal cardiac dimensions including GSI (cardiac length to cardiac width ratio), interventricular septum thickness (IVST), left ventricular wall thickness (LVWT) and right ventricular wall thickness (RVWT) were measured.
Results 215 pregnancies at risk met the inclusion criteria, including 54 affected fetuses and 161 normal fetuses. The mean GSI was significantly lower in the affected group (1.11 ± 0.06 vs. 1.26 ± 0.09, p-value 0.017). The GSI of the normal group was relatively constant regardless of gestational age. The IVST and LVWT tended to increase, but not significantly, in the affected group, whereas the RVWT was minimally but significantly increased. The ROC curve for GSI had an area under curve of 0.844. The best cut-off of GSI was 1.17, giving a sensitivity of 74.1 % and a specificity of 88.2 %.
Conclusion Fetal cardiac remodeling in response to anemia causes a marked decrease in global GSI with minimal hypertrophy as an adaption to volume overload. Importantly, GSI is a new maker for anemia and may play a role in clinical application for early detection of fetal anemia, possibly due to any cause. Additionally, GSI measurement is simple and gestational age-independent.
Septal cholinergic neurons gate hippocampal output to entorhinal cortex via oriens lacunosum moleculare interneurons
Authors: Haam Juhee , Zhou Jingheng , Cui Guohong , Yakel Jerrel L. 
Published: February 2018

Significance
Memory formation is a complex process that involves information transfer to the hippocampus for temporary storage (i.e., encoding) and the reciprocal circuit that relays the temporary information back to the neocortex for long-term storage (i.e., consolidation). Acetylcholine has been shown to play a critical role in memory function by differentially modulating encoding and consolidation, but the underlying mechanism is yet unclear. We found that acetylcholine suppresses the hippocampus–entorhinal cortex pathway, which is the gateway to the consolidation pathway. We show that this inhibition is mediated by oriens lacunosum moleculare interneurons and that the ablation of these interneurons impairs proper memory encoding. We provide evidence that demonstrates how acetylcholine tones down the memory consolidation pathway for efficient memory encoding.
Patient Predictors and Utilization of Health Services within a Medical Home for Homeless Persons
Authors: Jones Audrey L. , Thomas Roxanne , Hedayati Daniel O. , Saba Shaddy K. , Conley James , Gordon Adam J. 
Published: February 2018

Background: The Veterans Health Administration (VHA) established a patient-centered medical home model of care for veterans experiencing homelessness called a Homeless Patient Aligned Care Team (HPACT) to improve engagement with primary care and reduce utilization of hospital-based services. To evaluate the impact of the HPACT model, this study compares the number and type of health care visits in the 12 months before and after enrollment in HPACT at one VHA facility, and explores patient characteristics associated with increases and decreases in visits. Methods: Chart reviews of VHA medical records were conducted for all patients enrolled in an HPACT in Pittsburgh, Pennsylvania, between May 2012 and December 2013 ( N = 179). Multivariable mixed-effect logistic regressions estimated differences in having any visit in the 0–6 months and 7–12 months before and after HPACT enrollment, and multinomial logistic regressions predicted increases or decreases versus no change in number of visits over 12 months. Results: Compared with 0–6 months prior to HPACT, patients were more likely to visit primary care in the 0–6 months (adjusted odds ratio [aOR] = 4.91, 95% confidence interval [CI] = 2.94–8.20) and 7–12 months (aOR = 2.30, 95% CI = 1.42–3.72) following HPACT. Patients were less likely to visit the emergency department (ED) or to be hospitalized in the 0–6 months (aOR = 0.57, 95% CI = 0.34–0.94; and aOR = 0.55, 95% CI = 0.25–0.76) and 7–12 months (aOR = 0.43, 95% CI = 0.33–0.91; and aOR = 0.45, 95% CI = 0.26–0.80) following HPACT. Patients were less likely to visit mental health (aOR = 0.35, 95% CI = 0.20–0.60) and addiction specialists (aOR = 0.39, 95% CI = 0.18–0.84) in the 7–12 months following HPACT. Overall, 59% of patients had increases in primary care visits following HPACT. Female patients and those with self-housing were less likely to have increases versus no change in primary care visits (adjusted relative risk ratio [aRRR] = 0.15, 95% CI = 0.03–0.74; and aRRR = 0.35, 95% CI = 0.14–0.90). Conclusions: An integrated HPACT model was successful in engaging homeless veterans in primary care for 1 year, potentially contributing to reductions in ED use. More tailored approaches may be needed for vulnerable populations experiencing homelessness, including homeless women.
Repeated‐sprint performance and plasma responses following beetroot juice supplementation do not differ between recreational, competitive and elite sprint athletes
Authors: Jonvik K.L. , Nyakayiru J. , Van Dijk J.W. , Maase K. , Ballak S.B. , Senden J.M.G. , Van Loon L.J.C. , Verdijk L.B. 
Published: February 2018

AbstractPurpose: There is an ongoing debate whether highly trained athletes are less responsive to the ergogenic properties of nitrate. We assessed the effects of nitrate supplementation on plasma nitrate and nitrite concentrations and repeated‐sprint performance in recreational, competitive and elite sprint athletes. Methods: In a randomized double‐blinded cross‐over design, recreational cyclists (n = 20), national talent speed‐skaters (n = 22) and Olympic‐level track cyclists (n = 10) underwent two 6‐day supplementation periods; 140 mL/d nitrate‐rich (BR; ∼800 mg/d) and nitrate‐depleted (PLA; ∼0.5 mg/d) beetroot juice. Blood samples were collected and three 30‐s Wingate tests were performed. Results: Plasma nitrate and nitrite concentrations were higher following BR vs PLA (P < .001), with no differences between sport levels (all P > .10). Peak power over the three Wingates was not different between BR and PLA (1338 ± 30 vs 1333 ± 30 W; P = .62), and there was no interaction between treatment (BR‐PLA) and Wingate number (1‐2‐3; P = .48). Likewise, mean power did not differ between BR and PLA (P = .86). In contrast, time to peak power improved by ∼2.8% following BR vs PLA (P = .007). This improvement in BR vs PLA was not different between Wingate 1, 2 and 3. Moreover, the effects of BR vs PLA did not differ between sport levels for any Wingate parameter (all P > .30). Conclusion: The plasma and repeated‐sprint performance responses to beetroot juice supplementation do not differ between recreational, competitive and elite sprint athletes. Beetroot juice supplementation reduces time to reach peak power, which may improve the capacity to accelerate during high‐intensity and sprint tasks in recreational as well as elite athletes.
Study on the micro direct ethanol fuel cell (Micro-DEFC) performance
Authors: Saisirirat Penyarat , Joommanee Bordindech 
Published: February 2018

Abstract
The direct ethanol fuel cell (DEFC) is selected for this research. DEFC uses ethanol in the fuel cell instead of the more toxic methanol. Ethanol is more attractive than methanol by many reasons. Ethanol is a hydrogen-rich liquid and it has a higher specific energy (8.0 kWh/kg) compared to that of methanol (6.1 kWh/kg). Ethanol can be obtained in great quantity from biomass through a fermentation process from renewable resources such as sugar cane, wheat, corn, and even straw. The use of ethanol would also overcome both the storage and infrastructure challenge of hydrogen for fuel cell applications. The experimental apparatus on the micro direct ethanol fuel cell for measuring the cell performance has been set for this research. The objective is to study the micro direct ethanol fuel cell performance for applying with the portable electronic devices. The cell performance is specified in the terms of cell voltage, cell current and power of the cell at room operating temperature and 1 atm for the pressure and also includes the ethanol fuel consumption. The effect of operating temperature change on the electrical production performance is also studied. The steady-state time for collecting each data value is about 5-10 minutes. The results show that with the increase of concentrations of ethanol by volume, the reactant concentration at the reaction sites increases so the electrochemical rate also increases but when it reaches the saturated point the performance gradually drops.
The Proteomic Analysis of Pancreatic Exocrine Insufficiency Protein Marker in Type 2 Diabetes Mellitus Patients
Authors: Srihardyastutie Arie , Soeatmadji DW , Fatchiyah  , Aulanni’am  
Published: February 2018

Abstract
Type 2 Diabetes Mellitus (T2D) is the vast majority case of diabetes. Patient with T2D is at higher risk for developing acute or chronic pancreatitis. Prolonged hyperglycemia results in damages to tissue, which also causes dysfunctions of some organ systems, including enzyme or hormone secretions. Commonly, dysfunction or insufficiency of pancreatic exocrine is evaluated by increasing activity of serum pancreatic enzyme, such as amylase and lipase. Although incidence of pancreatitis was found in Indonesian T2D, the pathogenic mechanism still unclear. The aim of this study was to characterize the marker protein that indicated the correlation of pancreatic exocrine insufficiency with progression of T2D. Proteomic analysis using LC-MS/MS was used in identification and characterization of protein marker which indicates insufficiency pancreatic exocrine. First step, protein profile was analyzed by SDS-PAGE methods using serum sample of T2D compared with normal or healthy control, as negative control, and pancreatitis patients, as positive control. Protein with 18 kDa was found as a candidate protein marker which indicated the pancreatic exocrine insufficiency in T2D. The further identification of that protein using LC-MS/MS showed 4 peptide fragments. In silico analysis of the peptide fragment indicated the correlation of pancreatic exocrine insufficiency with progression of T2D was METTL10 – methyltransferase like protein-10.