bluuebunny/crossref_metadata_2025_split huggingface dataset:
In vitro, in vivo, and in silico approaches for evaluating the preclinical DMPK profiles of ammoxetine, a novel chiral serotonin and norepinephrine reuptake inhibitor
Authors: Zhu Xiuqing , Li Yuexin , Luo Huan , Zhang Yunxia , Zhang Zhenqing , Li Jinglai 
Published: November 2024

Background and AimAmmoxetine, a novel chiral serotonin and norepinephrine reuptake inhibitor, holds promise for major depressive disorder treatment. This study aimed to thoroughly investigate its preclinical drug metabolism and pharmacokinetics (DMPK) profiles.MethodsThe preclinical DMPK profiles of ammoxetine were examined through in vitro, in vivo, and in silico methods.ResultsAssessment of blood-brain barrier penetration via MDCK-MDR1 cells revealed strong brain permeation by ammoxetine, despite being a probable P-glycoprotein (P-gp) substrate. Molecular docking indicated a robust binding interaction between ammoxetine and P-gp. Ammoxetine was well absorbed orally, with Tmax ranging from 0.75 to 3.83 h in rats and 0.75–1.40 h in beagle dogs. At a 2 mg/kg dose in beagle dogs, ammoxetine exhibited an absolute bioavailability of approximately 42%. Plasma protein binding rates were around 50%–60% in beagle dogs, rats, and humans, suggesting moderate binding. Tissue distribution studies displayed rapid and extensive ammoxetine spread in major rat tissues post-gavage, with notable brain exposure and no tissue accumulation. Cumulative excretion rates in rats’ urine, feces, and bile accounted for only 1.11% of the total administered drug, indicating extensive transformation into metabolites. Chiral inversion of ammoxetine was absent in vivo. Metabolic stability varied across species using liver microsomes, but beagle dogs showed clearance rates more akin to humans. Metabolic pathways unveiled two key metabolites, M1 and M2. M1, likely generated through methylenedioxyphenyl ring oxidation, involves CYP2C19 and CYP3A4, crucial human cytochrome P450 (CYP) enzymes for liver metabolism, while M2 is M1’s glucuronide conjugate. Ammoxetine may exhibit saturation elimination trends with increasing doses in rats and beagle dogs. A high-throughput assay using the cocktail-substrate method indicated weak CYP inhibition by ammoxetine on CYP2D6 and CYP1A2, with minimal effects on other CYP enzymes, suggesting a low likelihood of CYP inhibition-related drug-drug interactions.ConclusionThis study presents encouraging DMPK profiles of ammoxetine, backing its potential as a candidate compound for future clinical assessments.
Impact of intergenerational support on older adults’ care expectations in rural areas in China
Authors: Wu Lianxia , Xie Tong , Guan Weihua , Li Wei 
Published: November 2024

IntroductionAs rural-to-urban migration accelerates, rural areas are experiencing a significant increase in empty-nesters among the older adults. Influenced by traditional concepts of filial piety, older adults in rural areas heavily rely on the family-based old care model, creating a complex interplay between supply and demand for older adults’ care. This study investigates the relationship between intergenerational support and older adults’ care expectations in rural areas. We construct measures of financial and care expectations of older adults’ care to reflect older adults’ intentions and choices.MethodsThis study uses data from the 2018 China Health and Retirement Longitudinal Study and rural case studies to employ a binomial logit regression model, moderation effect analysis, and in-depth interviews.ResultsOur research reveals that the level of financial expectations for older adults’ care is inversely related to the extent of financial support from the parental generation but positively related to the level of financial support from the offspring. However, the older adults’ care expectations do not appear to be influenced by intergenerational support. Furthermore, population characteristics of the parental generation, such as educational attainment, age, and marital status, moderate the relationship between intergenerational support and older adults’ care expectations. Educational attainment negatively moderates the impact of parental financial support on financial expectations, age positively moderates offspring’s financial support on financial expectations. However, age negatively moderates offspring’s financial support on care expectations, while marital status positively moderates offspring’s financial support on care expectations.DiscussionThese findings help to elucidate the older adults’ care expectations of rural residents during the process of urbanization and social transformation, offering family-centered solutions such as ‘promoting cohabitation or proximity of children to their parents, developing diverse older adults’ care models based on different family situations, and ensuring the provision of basic older adults’ care services’ to address the current challenges of rural older adults’ care in China.
Assessment of the relationship between living alone and suicidal behaviors based on prospective studies: a systematic review and meta-analysis
Authors: Luo Zhipeng , Wang Jinfeng , Chen Xu , Cheng Dejin , Zhou Yuanyuan 
Published: November 2024

Suicide, a global health concern, can be influenced by living arrangements. Hence, the objective of this systematic review was to assess the extent of the association between living alone and suicidal behaviors. We conducted a comprehensive search across eight databases for prospective studies. Hazard ratios (HR) and 95% confidence intervals were pooled using Stata software. Nine large-scale prospective studies with 3,663,205 participants proved eligible. The results of the meta-analysis showed that the pooled prevalence of living alone was 32%. Living alone was associated with suicide attempts and suicide death even after adjusting for all potential confounding factors (AHR = 1.45, 95% CI: 1.13–1.77; AHR = 1.27, 95% CI: 1.06–1.48). Compared to people who live with others, living alone increases the risk of suicide behaviors. Given the anticipated global rise in suicide rates and the growing prevalence of living alone, it is recommended to explore this issue on a broader scale.Systematic review registration: PROSPERO, identifier: CRD42023464684, https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42023464684.
Spatial prediction of the probability of liver fluke infection in water resource within sub-basin using an optimized geographically-weighted regression model
Authors: Pumhirunroj Benjamabhorn , Littidej Patiwat , Boonmars Thidarut , Artchayasawat Atchara , Buasri Nutchanat , Slack Donald 
Published: November 2024

IntroductionInfection with liver flukes (Opisthorchis viverrini) is partly attributed to their ability to thrive in sub-basin habitats, causing the intermediate host to remain within the watershed system throughout the year. It is crucial to conduct spatial monitoring of fluke infection at a small basin analysis scale as it helps in studying the spatial factors influencing these infections. The number of infected individuals was obtained from local authorities, converted into a percentage, and visually represented as raster data through a heat map. This approach generates continuous data with dependent variables.MethodsThe independent set comprises nine variables, including both vector and raster data, that establish a connection between the location of an infected person and their village. Design spatial units optimized for geo-weighted modeling by utilizing a clustering and overlay approach, thereby facilitating the optimal prediction of alternative models for infection.Results and discussionThe Model-3 demonstrated the strongest correlation between the variables X5 (stream) and X7 (ndmi), which are associated with the percentage of infected individuals. The statistical analysis showed t-statistics values of −2.045 and 0.784, with corresponding p-values of 0.016 and 0.085. The RMSE was determined to be 2.571%, and the AUC was 0.659, providing support for these findings. Several alternative models were tested, and a generalized mathematical model was developed to incorporate the independent variables. This new model improved the accuracy of the GWR model by 5.75% and increased the R2 value from 0.754 to 0.800. Additionally, spatial autocorrelation confirmed the difference in predictions between the modeled and actual infection values. This study demonstrates that when using GWR to create spatial models at the sub-basin level, it is possible to identify variables that are associated with liver fluke infection.
Relationship Between the Online Health Information Search and Vaccination Behavior During the COVID-19 Pandemic
Authors: Xu Yunpeng , Pan Chen , Kong Peiyu , Shangguan Lina 
Published: November 2024

ObjectivesDuring the COVID-19 pandemic, online health information search has been shown to influence the public’s health beliefs, risk attitudes, and vaccination behavior. This study constructs a conditional process model to explore how online health information search impacts public vaccination behavior, considering critical factors like healthcare system satisfaction, vaccine risk perception, and the perceived usefulness of information.MethodsData from the 2021 Chinese General Social Survey (N = 2,547) were analysed. The study utilized logistic regression, path analysis, and the Bootstrap method to test the conditional process model.ResultsIncreased online health information search promotes vaccination behavior, while increased vaccine risk perception hinders vaccination behavior. Higher satisfaction with the healthcare system encourages vaccination behavior, but online health information search reduces healthcare system satisfaction. Satisfaction with the healthcare system and vaccine risk perception play a chain mediating role between online health information search and vaccination behavior. Additionally, the perceived usefulness of information has a negative moderating effect on online health information search and healthcare system satisfaction.ConclusionThe research findings provide new insights for health information dissemination and vaccination interventions.
Biological Nitrification Inhibition by Australian Tussock Grass and Its Impact on the Rhizosphere Ammonia-Oxidizing Microbiome
Authors: Zhou Yi , Toh Ruey , Iqbal Nasir , Ryder Maarten , Li Jishun , Denton Matthew D. 
Published: November 2024

Certain plant species have developed the ability to express biological nitrification inhibition (BNI), suppressing the activity of nitrifying microbes and thereby reducing the conversion of ammonium to nitrate. This study assessed the BNI capacity and the rhizosphere ammonia-oxidizing microbiome of two grass species: the endemic Australian Barley Mitchell grass (Astrebla pectinata) and the introduced koronivia grass (Urochloa humidicola), using soils from both agricultural land and native vegetation. In agricultural soil, koronivia grass exhibited significantly higher BNI capacity compared with Barley Mitchell grass. However, in native soil, this trend was reversed, with Barley Mitchell grass demonstrating a significantly greater BNI capacity than koronivia grass (52% vs. 38%). Koronivia grass significantly altered the composition of the ammonia-oxidizing bacteria community in its rhizosphere, leading to a decrease in the Shannon index and bacteria number. Conversely, Barley Mitchell grass reduced the Shannon index (1.2 vs. 1.7) and population size (3.28 × 107 vs. 7.43 × 107 gene copy number g−1 dry soil) of the ammonia-oxidizing archaea community in its rhizosphere to a greater extent. These findings suggest that Australian Barley Mitchell grass may have evolved mechanisms to suppress soil archaeal nitrifiers, thereby enhancing its BNI capacity and adapting to Australia’s nutrient-poor soils.
AaSlt2 Is Required for Vegetative Growth, Stress Adaption, Infection Structure Formation, and Virulence in Alternaria alternata
Authors: Jiang Qianqian , Wang Tiaolan , Li Yongcai , Bi Yang , Zhang Miao , Wang Xiaojing , Prusky Dov B. 
Published: November 2024

Slt2 is an important component of the Slt2-MAPK pathway and plays critical regulatory roles in growth, cell wall integrity, melanin biosynthesis, and pathogenicity of plant fungi. AaSlt2, an ortholog of the Saccharomyces cerevisiae Slt2 gene, was identified from A. alternata in this study, and its function was clarified by knockout of the gene. The ΔAaSlt2 strain of A. alternata was found to be defective in spore morphology, vegetative growth, and sporulation. Analysis of gene expression showed that expression of the AaSlt2 gene was significantly up-regulated during infection structure formation of A. alternata on hydrophobic and pear wax extract-coated surfaces. Further tests on onion epidermis confirmed that spore germination was reduced in the ΔAaSlt2 strain, together with decreased formation of appressorium and infection hyphae. Moreover, the ΔAaSlt2 strain was sensitive to cell wall inhibitors, and showed significantly reduced virulence on pear fruit. Furthermore, cell wall degradation enzyme (CWDE) activities, melanin accumulation, and toxin biosynthesis were significantly lower in the ΔAaSlt2 strain. Overall, the findings demonstrate the critical involvement of AaSlt2 in growth regulation, stress adaptation, infection structure formation, and virulence in A. alternata.
Prediksi Jumlah Kunjungan Pasien Mental disorder Pelayanan Mentari Puskesmas Kecamatan Kalideres menggunakan ARCH, GARCH dan Box-Jenkins
Authors: Arifin Alicia , Wiyanti Wiwik 
Published: November 2024

Pelayanan Mentari adalah pelayanan pasien mental disorder di Puskesmas kecamatan Kalideres, yang mana memiliki sub pelayanan Polania (poli anti aniaya) untuk menangani permasalahan bullying pada. Penelitian ini dilakukan untuk memprediksi jumlah kunjungan pasien di pelayanan Mentari. Data yang ditemukan menunjukkan bahwa 26,7% remaja di Indonesia mengalami gangguan cemas dan total prevalensi masalah kesehatan mental mencapai 46,8%, penting untuk mengidentifikasi pola data agar pemerintah bisa mengatasi masalah mental disorder sejak dini. Jenis penelitian yang digunakan adalah metode kuantitatif. Tujuan dari penelitian ini adalah menggambarkan kondisi kesehatan mental di daerah Kalideres dan bisa memproyeksikan jumlah pasien mental disorder di daerah Kalideres, sehingga pemerintah bisa membuat kebijakan untuk menurunkan masalah mental disorder. Data yang digunakan adalah data bulanan kunjungan pasien mental disorder pelayanan Mentari tahun 2020-2023. Metode analisa time-series yang digunakan adalah Box-Jenkins, ARCH dan GARCH. Metode yang terbaik adalah metode Box-Jenkins dengan ARIMA(1,1,1) yang mempunyai nilai MAPE sebesar 9,7%. Prediksi di bulan januari 2024 adalah 270,60 (95% CI, 204,97-336,23) dan bulan Februari 2024 sebanyak 269.31 (95% CI, 200,77 - 337,86). Hasil prediksi oleh model ARCH(1) sebesar 200,97(95% CI, -206,28-608,22), model GARCH(1,1) sebesar 191,95 (95% CI, -215,30-599,20).
Kata kunci: mental disorder, forecast, arima, arch, garch
Ride-Hailing Drivers' Preferences for Fairwork Principles of Satisfactory Working Conditions and Fair Pay-Profit Distribution and Willingness to Form and Join Worker-Platform Co-Operatives in Kenya
Authors: Matabi Jared Mark Ochieno , Gicheru Esther Njoki , Kiganane Lucy Maina 
Published: November 2024

Ride-hailing drivers (e-drivers) are among the platform workers who successfully embrace the worker-platform co-operative model's emergence to counter capitalistic ride-hailing platforms and their complex ecosystems, which align with fairwork principles of pay, condition, contracts and (platform) management. This study was conducted to determine the extent of ride-hailing drivers' preferences for satisfactory working conditions, fair pay-profit distribution and willingness to form and join worker-platform co-operatives in Kenya. The study was grounded in the social exchange theory, affective theory of social exchange, and utility theory. The researcher applied critical-realism research philosophy and multimethod research designs of discrete choice experiments and sequential explanatory mixed method research designs. Using the Yamane sampling formula, the study used quantitative data from 497 respondents from sampled 600 of the 20,000 e-drivers in the Nairobi Metropolitan Region. The quantitative data was analysed using multinominal logistic regression. Moreover, the study used qualitative data from 11 participants, which was analysed using thematic analysis. Results indicated that the two fairwork principles were statistically significant to e-drivers’ willingness to form and join worker-platform co-operatives. fair pay-profits distribution (FPD) principle, in particular, was a highly preferred fairwork principle in terms of e-drivers’ willingness to form and join the worker-platform co-operative model (Coeff=1.564, SE = 0.234, Z = 6.67, p< .001), compared to the satisfactory working conditions (SWC) principle (Coeff=0.783, SE = 0.156, Z = 5.012, p< .001). This study brings practical and theoretical contributions to improve the e-drivers’ benefits from the ride-hailing sector. Policymakers, promoters, and platform workers like e-drivers could understand how best to promote worker-platform co-operatives to succeed in the country’s societal context.
ERAS (Enhanced Recovery After Surgery) Protocol in Oral and Maxillofacial Surgery: Narrative Review
Authors: Anderson Jara Ferreira  , Camila Coêlho Guimarães  , Isabella Bezerra Araújo Cirilo  , Maria Eduarda Batista Henriques  , Karolayne Dutra Félix  , Dárcio Brito da Cunha  , Etiene Pereira Duarte Lins  , HUDSON GOMES DE SANTANA ILAN , Arthur Felipe de Brito Andrade  , Maria Claúdia Oliveira da Silva  , Erick Andres Alpaca  , Mayara Rebeca Martins Viana  
Published: November 2024

Introduction: Oral and maxillofacial surgery is a specialty that involves the diagnosis and treatment of pathologies related to the oral cavity, mandible, jaws and facial structures. In recent years, the ERAS (Enhanced Recovery After Surgery) Protocol has been implemented in various surgical areas with the aim of optimizing postoperative recovery. Objective: To understand the impact of this approach on the patient experience and clinical outcomes. Methodology: This narrative review was conducted through a systematic search of the PubMed, Scopus and Google Scholar databases. The aim was to identify studies investigating the implementation of the ERAS Protocol in oral and maxillofacial surgery. Results: The studies reviewed showed that the implementation of the ERAS Protocol in oral and maxillofacial surgery resulted in a significant reduction in the length of hospital stay, with patients being discharged in 1 to 2 days. In addition, pain control was improved, with a reduction in the use of opioids by up to 50%. The reduction in post-operative complications, such as infections and respiratory problems, was another benefit observed, with a reduction of up to 40% in these complications. Discussion: The adoption of the ERAS Protocol has shown promising results in the recovery of patients undergoing oral and maxillofacial surgery. The benefits include better pain management, early mobilization and lower complication rates. Conclusion: The ERAS Protocol in oral and maxillofacial surgery has the potential to transform surgical practice, promoting faster and more effective recovery. More studies are needed to standardize its implementation and evaluate its long-term effects.
A Consensus on Current Issues of Multidisciplinary Care for Geriatric Patients with Frailty Undergoing Routine Surgical Procedures
Authors: Melkonyan G. G. , Protsenko D. N. , Runikhina N. K. , Tkacheva O. N. , Revishvili A. Sh. , Tsarenko S. V. , Eruslanova K. A. 
Published: November 2024

The demographic shift towards an increasing number of older and oldest-old individuals is a global phenomenon. This trend has been accompanied by a rise in surgical interventions targeting individuals aged 65 years and above.However, the traditional clinical approach to treating single diseases often proves insufficient for geriatric patients due to multiple factors, such as the presence of multiple chronic conditions, the use of multiple medications, physical frailty, malnutrition, cognitive impairments, and diminished physiological function. With age, metabolic processes tend to slow down, leading to reduced efficiency and diminished ability to cope with stressors.These factors, coupled with the increasing frequency of surgical interventions, pose a significant risk of postoperative complications and even mortality. Consequently, a comprehensive approach is essential for geriatric surgical patients. This approach encompasses preoperative assessment, meticulous surgical planning, and complex postoperative care. A multidisciplinary team of medical professionals is indispensable for providing comprehensive care throughout the treatment course.
Hormonal and metabolic status of centenarians diagnosed with sarcopenia: Prospective, Observational Study
Authors: Matchekhina L. V. , Tkacheva O. N. , Shelley E. M. , Mamchur A. A. , Erema V. V. , Bruttan M. V. , Ivanov M. V. , Kashtanova D. A. , Rumyantseva A. M. , Yudin V. S. , Makarov V. V. , Keskinov A. A. , Kraevoy S. A. , Yudin S. M. , Strazhesko I. D. 
Published: November 2024

RELEVANCE: Sarcopenia is one of the leading geriatric syndromes that increases the risk of disability, falls and injuries. This syndrome is of particular importance for centenarians aged 90 years and older, for whom a detailed analysis of the course of sarcopenia has not previously been carried out and the hormonal and metabolic characteristics of this condition have not been described.AIM OF THE STUDY: To analyze the features of hormonal and metabolic status in nonagenarians with sarcopenia and to identify factors that increase the risk of developing this conditionMATERIALS AND METHODS: The study included 2221 people over the age of 90 years. Study participants underwent a comprehensive geriatric assessment during a visit with a doctor and nurse, as well as blood tests to measure a number of metabolic and hormonal parameters. Statistical data analysis was carried out using the R programming language version 4.2.2.RESULTS: Apolipoprotein A1, free triiodothyronine, vitamin D, albumin, C-reactive protein, hemoglobin, red blood cells and hematocrit were significantly associated with the presence of sarcopenia. In a survival analysis of people with sarcopenia, the strongest protective factors for participants with sarcopenia were any physical activity and increasing free T3. Malnutrition is the leading destructive factor.CONCLUSION: Hormonal metabolic status, in particular low concentrations of vitamin D, triiodothyronine, albumin, and apolipoprotein A1, largely determines the presence of sarcopenia in centenarians, but when assessing risks, it is necessary to take into account a number of other important parameters, such as physical activity and nutritional status.