bluuebunny/crossref_metadata_2025_split huggingface dataset:
Two-year dynamic functional network connectivity in clinically isolated syndrome
Authors: Rocca Maria A , Hidalgo de La Cruz Milagros , Valsasina Paola , Mesaros Sarlota , Martinovic Vanja , Ivanovic Jovana , Drulovic Jelena , Filippi Massimo 
Published: March 2019

Background: The features of functional network connectivity reorganization at the earliest stages of MS have not been investigated yet. Objective: To combine static and dynamic analysis of resting state (RS) functional connectivity (FC) to identify mechanisms of clinical dysfunction and recovery occurring in clinically isolated syndrome (CIS) patients. Methods: RS functional magnetic resonance imaging (fMRI) and clinical data were prospectively acquired from 50 CIS patients and 13 healthy controls (HC) at baseline, month 12 and month 24. Between-group differences and longitudinal evolution of network FC were analysed across 41 functionally relevant networks. Results: At follow-up, 47 patients developed MS. Disability remained stable (and relatively low). CIS and HC exhibited two recurring RS FC states (states 1 and 2, showing low and high internetwork connectivity, respectively). At baseline, patients showed reduced state 2 connectivity strength in the default-mode and cerebellar networks, and no differences in global dynamism versus HC. A selective FC reduction in networks affected by the clinical attack was also detected. At follow-up, increased state 2 connectivity strength and global connectivity dynamism was observed in patients versus HC. Conclusion: Longitudinal FC modifications occurring relatively early in the course of multiple sclerosis may represent a protective mechanism contributing to preserve clinical function over time.
Evaluation on Short-Term Therapeutic Effect of 2 Porphyrin Photosensitizer-Mediated Photodynamic Therapy for Esophageal Cancer
Authors: Liu Huilong , Liu Yanfang , Wang Li , Ruan Xinjian , Wang Fei , Xu Dandan , Zhang Jing , Jia Xiaoyan , Liu Duanqi 
Published: March 2019

Purpose: To analyze and study the short-term therapeutic effects and main adverse effects of 2 Porphyrin photosensitizer-mediated photodynamic therapy for esophageal cancer. Methods: We apply the hematoporphyrin derivative and hematoporphyrin injection produced by different manufacturers at different periods as photosensitizers in therapy of 79 esophageal cancer cases, with the administration dosage of 5 mg/kg and intravenous drip 24 hours before irradiation. We apply the gold vapor laser and semiconductor laser, respectively, as treatment light source, with the power density of 100 to 300 mW/cm2 and energy density of 100 to 300 J/cm2. After treatment for 1 to 4 sessions, we evaluate the short-term therapeutic effects as complete response, partial response, minor response, or no change, and then make comparative study on therapeutic effects and adverse effects. Results: There were 47 patients in hematoporphyrin derivative group, including 3 (6.4%) patients with complete response, 31 (66.0%) patients with partial response, 10 (21.3%) patients with minor response, and 3 (6.4%) patients with no change. The dysphagia score was reduced from 2.53 (1.16) before treatment to 1.32 (1.20; P < .01) after treatment. There were 32 patients in the hematoporphyrin injection group, including 3 (9.4%) patients with complete response, 19 (59.4%) patients with partial response, 6 (18.8%) patients with minor response, and 4 (12.5%) patients with no change. The dysphagia score was reduced from 2.41 (1.13) before treatment to 1.18 (0.99; P < .01) after treatment. The dysphagia scores of 2 groups after treatment were significantly reduced compared to those before treatment. Both groups did not display serious adverse effect. Conclusions: Two porphyrin photosensitizers in treatment of esophageal cancer at different clinical stages all had good effect with similar therapeutic effect, mild adverse effect, and good tolerance, which implies it is a preferable palliative therapy means.
Left Internal Thoracic Artery Graft Assessment by Firefly Fluorescence Imaging for Robot-Assisted Minimally Invasive Direct Coronary Artery Bypass
Authors: Nakamura Yoshitsugu , Kuroda Miho , Ito Yujiro , Masuda Takahiko , Nishijima Shuhei , Hirano Takahisa , Hisasue Shinichi 
Published: March 2019

Objective The da Vinci Xi surgical system (Intuitive Surgical, Sunnyvale, CA, USA) cannot give tactile feedback to surgeons. This shortcoming may increase the risk of left internal thoracic artery (LITA) injury during its harvest. We utilized Firefly Fluorescence Imaging (Firefly) to assess LITA quality in robot-assisted minimally invasive direct coronary artery bypass (R-MIDCAB). Methods We retrospectively reviewed clinical records and intraoperative videos of 30 consecutive patients who underwent R-MIDCAB with LITA–left anterior descending (LAD) coronary bypass. All patients had post-harvest assessment of LITA blood flow by Firefly with 1 mL (2.5 mg/mL) of indocyanine green injection through a central line. Results Twenty-seven of the patients were male, mean age was 67.7 ± 10.7 years. In post-harvest assessment performed before transection of the distal LITA, blood flow in LITA was well visualized in 28 patients. In the remaining 2 patients, 1 had dissection and the other had severe spasm of the LITA. Firefly was also useful for locating LITA and LAD and for assessing blood flow of the graft after anastomosis. Time required for each Firefly assessment was approximately 20 seconds. There were no side effects or complications due to Firefly intraoperatively and postoperatively. Twenty-six patients had postoperative coronary computed tomography; LITA patency rate was 100% (26/26). Conclusion Firefly is fast, simple, and effective for locating and assessing flow in LITA and LAD before and after anastomosis in R-MIDCAB.
Clinical and Functional Outcomes Associated with Age after Transapical Transcatheter Aortic Valve Replacement
Authors: Okoh Alexis Kofi , Kang Nathan , Haik Nicky , Fugar Setri , Chunguang Chen , Bruce Haik , Cohen Marc , Russo Mark J. 
Published: March 2019

Objective Transcatheter aortic valve replacement (TAVR) via a transapical (TA) approach has been associated with high morbidity. The aim of this study is to investigate the association of age and clinical and functional outcomes after TA-TAVR. Methods Patients who had TA-TAVR at a single center were divided into 3 age groups: <75 years (Group I), 75 to 85 years (Group II), and >85 years (Group III). Pre- and postoperative clinical, functional status, and procedure-related outcomes were compared among patient groups. A multivariable Cox proportional hazards model was used to assess the impact of age on overall all-cause mortality. Results Out of 183 TA-TAVR cases performed, 117 met the study criteria. These included 15 aged <75 years, 60 aged 75 to 85 years, and 42 aged >85 years. Short-term (30-day) clinical and functional status improved significantly for all age groups. The incidence of acute kidney injury, access site complications, and requirement for permanent pacemaker were similar for all age groups at 30 days. After a median follow-up of 26 months, overall all-cause survival rates were 86% for Group I, 88% for Group II, and 83% for Group III at 1 year. Cox proportional hazards model showed frailty status (HR: 1.84; 95% CI, 1.23 to 2.69; P = 0.003) but not age as an independent predictor of overall all-cause mortality. Conclusions Findings from this study suggest that both older and younger patients benefit from TA-TAVR with comparable operative outcomes. Age should not be an exclusion criterion for TA-TAVR.
Novel CD19-targeted TriKE restores NK cell function and proliferative capacity in CLL
Authors: Felices Martin , Kodal Behiye , Hinderlie Peter , Kaminski Michael F. , Cooley Sarah , Weisdorf Daniel J. , Vallera Daniel A. , Miller Jeffrey S. , Bachanova Veronika 
Published: March 2019

Abstract
Chronic lymphocytic leukemia (CLL) is characterized by chronic clonal expansion of mature CD19-expressing B lymphocytes and global dysfunction of immune effectors, including natural killer (NK) cells. CLL remains incurable, and novel approaches to refractory CLL are needed. Our group has previously described trispecific killer engager (TriKE) molecules that redirect NK cell function against tumor cells. TriKE reagents simultaneously bind an activating receptor on NK cells, CD16, and a tumor antigen while also providing an NK cell expansion signal via an interleukin-15 moiety. Here we developed the novel CD19-targeting 161519 TriKE. We demonstrate that 161519 TriKE induced killing of a CD19-expressing Burkitt’s lymphoma cell line and examined the impact on primary CLL targets using healthy donor and patient NK cells. 161519 TriKE induced potent healthy donor NK cell activation, proliferation, and directed killing. Furthermore, 161519 TriKE rescued the inflammatory function of NK cells obtained from CLL patient peripheral blood samples. Finally, we show that 161519 TriKE induced better directed killing of CLL in vitro when compared with rituximab. In conclusion, 161519 TriKE drives a potent activating and proliferative signal on NK cells, resulting in enhanced NK cell expansion and CLL target killing. Our findings indicate the potential immunotherapeutic value of 161519 TriKE in CLL.
Combined photoluminescence and Raman microscopy for the identification of modern pigments: explanatory examples on cross-sections from Russian avant-garde paintings
Authors: Artesani Alessia , Ghirardello Marta , Mosca Sara , Nevin Austin , Valentini Gianluca , Comelli Daniela 
Published: March 2019

Abstract
In conservation science, the identification of painting materials is fundamental for the study of artists’ palettes, for dating and for understanding on-going degradation phenomena. For these purposes, the study of stratigraphic micro-samples provides unique information on the complex heterogeneity of the pictorial artworks. In this context, we propose a combined-microscopy approach based on the application of time-resolved photoluminescence (TRPL) micro-imaging and micro-Raman spectroscopy. The TRPL device is based on pulsed laser excitation (excitation wavelength = 355 nm, 1 ns pulse width) and time-gated detection, and it is suitable for the detection of photoluminescent emissions with lifetime from few nanoseconds to hundreds of microseconds. In this work, the technique is beneficially applied for identifying different luminescent semiconductor and mineral pigments, on the basis of their spectral and decay kinetic emission properties. The spatial heterogeneities, detected in the micro-sample, are investigated with Raman spectroscopy (785-nm in CW mode) for a further identification of the paint composition on basis of the molecular vibrations associated with the crystal structure. The effectiveness and limits of the proposed combined method is discussed through analysis of a corpus of stratigraphic micro-samples from Russian Avant-garde modern paintings. In the selected samples, the method allows the identification of modern inorganic pigments such as cadmium-based pigments, zinc white, titanium white, chrome yellow, ultramarine and cinnabar.
Weighing the Evidence: Variant Classification and Interpretation in Precision Oncology, US Food and Drug Administration Public Workshop—Workshop Proceedings
Authors: Horne Hisani N. , Roscoe Donna , Litwack E. David , Pathak Anand , Pandey Jai P. , Gallagher Pamela S. , Koontz Laura M. , Schuck Robert N. , Lee Eunice Y. , Hu Yun-Fu , Beaver Julia A. , Blumenthal Gideon M. , Philip Reena 
Published: March 2019

PURPOSE Next-generation sequencing (NGS) oncology panels are becoming integral in hospital and academic settings to guide patient treatment and enrollment in clinical trials. Although NGS technologies have revolutionized decision-making for cancer therapeutics, physicians may face many challenges in parsing and prioritizing NGS-based test results to determine the best course of treatment for individual patients. On January 29, 2018, the US Food and Drug Administration held a public workshop entitled, “Weighing the Evidence: Variant Classification and Interpretation in Precision Oncology.” Here, we discuss the presentations and discussion highlights across the four sessions of the workshop. METHODS The goal of the public workshop was to engage stakeholders and solicit input from experts in precision oncology to discuss the integration of complex NGS data into patient management and regulatory innovation within the precision oncology community. The US Food and Drug Administration gathered representatives from academia, industry, patient advocacy, government, and professional organizations for a series of presentations followed by panel discussions. After the workshop, the transcript and speaker presentation slides were reviewed and summarized for manuscript preparation. RESULTS Speakers and panelists provided diverse perspectives on the integration of NGS technology into patient care for oncology and on the complexities that surround data interpretation and sharing. Discussions highlighted the challenges with standardization for variant classification while expressing the utility of consensus recommendations among stakeholders in oncology for driving innovation in the era of precision medicine. CONCLUSION As precision medicine advances, clear communication within the field of precision oncology will be key to creating an environment that facilitates the generation and sharing of data that have value to patients. [Box: see text]
The Hunchback temporal transcription factor determines motor neuron axon and dendrite targeting in Drosophila
Authors: Seroka Austin Q. , Doe Chris Q. 
Published: March 2019

ABSTRACT
The generation of neuronal diversity is essential for circuit formation and behavior. Morphological differences in sequentially born neurons could be due to intrinsic molecular identity specified by temporal transcription factors (henceforth called intrinsic temporal identity) or due to changing extrinsic cues. Here, we have used the Drosophila NB7-1 lineage to address this issue. NB7-1 generates the U1-U5 motor neurons sequentially; each has a distinct intrinsic temporal identity due to inheritance of different temporal transcription factors at its time of birth. We show that the U1-U5 neurons project axons sequentially, followed by sequential dendrite extension. We misexpressed the earliest temporal transcription factor, Hunchback, to create ‘ectopic’ U1 neurons with an early intrinsic temporal identity but later birth-order. These ectopic U1 neurons have axon muscle targeting and dendrite neuropil targeting that are consistent with U1 intrinsic temporal identity, rather than with their time of birth or differentiation. We conclude that intrinsic temporal identity plays a major role in establishing both motor axon muscle targeting and dendritic arbor targeting, which are required for proper motor circuit development.
Nuclear actin interactome analysis links actin to KAT14 histone acetyl transferase and mRNA splicing
Authors: Viita Tiina , Kyheröinen Salla , Prajapati Bina , Virtanen Jori , Frilander Mikko J. , Varjosalo Markku , Vartiainen Maria K. 
Published: March 2019

ABSTRACT
In addition to its essential functions within the cytoskeleton, actin also localizes to the cell nucleus, where it is linked to many important nuclear processes from gene expression to maintenance of genomic integrity. However, the molecular mechanisms by which actin operates in the nucleus remain poorly understood. Here, we have used two complementary mass spectrometry (MS) techniques, AP-MS and BioID, to identify binding partners for nuclear actin. Common high-confidence interactions highlight the role of actin in chromatin-remodeling complexes and identify the histone-modifying complex human Ada-Two-A-containing (hATAC) as a novel actin-containing nuclear complex. Actin binds directly to the hATAC subunit KAT14, and modulates its histone acetyl transferase activity in vitro and in cells. Transient interactions detected through BioID link actin to several steps of transcription as well as to RNA processing. Alterations in nuclear actin levels disturb alternative splicing in minigene assays, likely by affecting the transcription elongation rate. This interactome analysis thus identifies both novel direct binding partners and functional roles for nuclear actin, as well as forms a platform for further mechanistic studies on how actin operates during essential nuclear processes.
This article has an associated First Person interview with the first author of the paper.
Clinical outcomes of zirconia-reinforced lithium silicate partial coverage crowns compared to lithium disilicate partial coverage crowns. A randomized controlled split-mouth clinical study
Authors: Nassar Hanaa , H Halim Carl , A Katamish Hesham 
Published: March 2019

Background: Despite the fact that preliminary clinical results of conservative partial coverage restorations (PCRs) are promising, the clinical behavior of different PCR ceramic materials is rarely investigated in clinical trials. This study aimed to evaluate the clinical outcomes of partial coverage restorations (PCR) fabricated with zirconia-reinforced lithium silicate ceramic system compared to partial coverage restorations fabricated with lithium disilicate ceramic system. Methods: 46 vital premolars and molars of 14 patients were restored with PCRs (23 Vita Suprinity and 23 IPS e.max CAD). PCRs were CAD/CAM fabricated in the lab and adhesively luted with dual-polymerizing resin cement (Duolink. BISCO, USA). Clinical evaluation of PCRs was performed according to the Modified United States Public Health Service (USPHS) at baseline, 6 and 12 months post-insertion. Absolute failure was demonstrated by Kaplan-Meier survival rate analysis. Results: After 12 months observation, all PCRs of both ceramic groups demonstrated 100% survival rate. Non-significant decrease in Alpha ratings for marginal adaptation (p = 0.1560) and marginal discoloration (p = 0.6078) in e-max group. While in the Suprinity group, PCRs demonstrated 100% Alpha ratings for marginal adaptation and only one Bravo rating (p= 0.3625) for marginal discoloration after 12 month observation. Conclusions: Both Vita-Suprinity and e.max CAD partial coverage restorations are considered reliable treatment options for restoring larger defects in posterior dentition. Trial registration: ClinicalTrials.gov NCT02861729 04/08/2016
Technology Conglomeration, Strategic Alliances, and Corporate Innovation
Authors: Li Kai , Qiu Jiaping , Wang Jin 
Published: March 2019

We examine the organizational choice and innovative activity of technology conglomerates—firms that explore different technology fields with heated inventive activity. We develop a measure of firm-to-economy technological proximity to capture the extent of a firm’s technology conglomeration. We show that technology conglomerates are more likely to form alliances and that these alliances lead to higher patent output. In terms of underlying mechanisms, we show that after alliance formation, there are significant knowledge pooling and cross-fertilization between technology conglomerates and their alliance partners. Moreover, technology conglomerates produce more patents that are novel and/or with greater impact. Our findings suggest that both synergy and tolerance for failure are important motives for technology conglomerates to use alliances to accelerate corporate innovation. This paper was accepted by Gustavo Manso, finance.
ANALISIS PENGARUH PENGINDERAAN PASAR, PEMASARAN KEWIRAUSAHAAN, KEMAMPUAN JARINGAN, DAN KEUNGGULAN BERSAING TERHADAP KINERJA PEMASARAN (Studi Pada UKM Makanan dan Minuman di Kota Semarang)
Authors: Choirunnisa Choirunnisa , Ferdinand Augusty Tae , Indriani Farida 
Published: March 2019

Penelitian ini bertujuan untuk menguji apakah pengaruh penginderaan pasar, pemasaran kewirausahaan, kemampuan jaringan, dan keunggulan bersaing mempunyai pengaruh terhadap kinerja pemasaran pada UKM makanan dan minuman di Kota Semarang. Penelitian ini mengambil sampel sebanyak 200 responden UKM makanan dan minuman di Kota Semarang. Teknik pengambilan sampel yang digunakan yaitu probability sampling dengan metode simple random sampling. Alat analisis yang digunakan yaitu structural equation model (SEM) pada program AMOS. Hasil analisis data untuk model secara keseluruhan telah memenuhi kriteria Goodness Of Fit yaitu nilai chi-square = 213,800; probability = 0,059; CMIN/DF = 1,168; GFI = 0,901; AGFI = 0,875; TLI = 0,988; CFI = 0,989; RMSEA = 0,029. Dengan demikian, dapat dikatakan bahwa model penelitian yang diajukan dapat diterima. Penelitian ini mempunyai lima hipotesis dan sudah diuji, ada satu hipotesis yang ditolak dan empat diterima. H1 penginderaan pasar memiliki pengaruh positif dan signifikan terhadap keunggulan bersaing. Kemudian, H2 pemasaran kewirausahaan memiliki pengaruh positif dan signifikan terhadap kemampuan jaringan, lalu H3 keunggulan bersaing memiliki pengaruh positif dan signifikan terhadap kinerja pemasaran. Selanjutnya H4 kemampuan jaringan memiliki pengaruh positif dan signifikan terhadap keunggulan bersaing, dan H5 kemampuan jaringan memiliki pengaruh positif dan tidak signifikan terhadap kinerja pemasaran.
The Quintessence of Organizational Commitment and Organizational Cynicism
Authors: Margelytė-Pleskienė Aida , Vveinhardt Jolita 
Published: March 2019

Abstract
The article deals with the constituents of organizational commitment and organizational cynicism and in order to concisely present systematised material disclosing the very essence of the phenomena, the authors analyse their interrelation, the specificity of development (organizational commitment) and management (organizational cynicism), their causes, importance and / or consequences caused. The aim of research is to distinguish the dimensions of the relationship of these phenomena highlighting the quintessence of organizational commitment and organizational cynicism.
Presumptions of Decentralisation in the Context of Institutional and Political Economy
Authors: Žigienė Gerda 
Published: March 2019

Abstract
The aim of the article is to discuss and distinguish the notion of decentralisation within time, institutional economics and political organisation of States, as well as the role of decentralisation in the context of those political organisations. The differences between the notions of decentralisation and federalism are differentiated in order to avoid misinterpretations of the notions.
Crystal structure of (3E,5E)-3,5-bis(4-cyanobenzylidene)-1-((4-fluorophenyl)sulfonyl)piperidin-4-one, C27H18FN3O3S
Authors: Li Xiao-Yan , Meng Qing-Guo , Hou Gui-Ge 
Published: March 2019

Abstract
C27H18FN3O3S, monoclinic, P21/c (no. 14), a = 8.091(6) Å, b = 42.84(3) Å, c = 7.096(6) Å, β = 107.806(10)°, V = 2342(3) Å3, Z = 4, R
gt(F) = 0.0616, wR
ref(F
2) = 0.1321, T = 173(2) K.