bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of ferulic acid esterase-producingLactobacillus fermentumand cellulase additives on the fermentation quality and microbial community of alfalfa silage
Authors: Su Rina , Ni Kuikui , Wang Tianwei , Yang Xiaopan , Zhang Jie , Liu Yayong , Shi Weixiong , Yan Liu , Jie Chen , Zhong Jin 
Published: October 2019

BackgroundAlfalfa (Medicago sativa) is an important forage material widely used for animal feed production. Ensiling is an effective method for preserving alfalfa, but it has shown some limitations in the production of high-quality alfalfa silage due to its low water soluble carbohydrates (WSC) content and high buffering capacity. Lactic acid bacteria (LAB) and cellulase are often used as silage additives to promote the ensiling process and enhance fermentation quality.MethodsExperiments were conducted to investigate the effects of ferulic acid esterase (FAE)-producingLactobacillus fermentum17SD-2 (LF) and cellulase (CE) on the fermentation quality and microbial community of alfalfa silage. After 60 days of ensiling, analysis of fermentation quality and bacterial diversity in alfalfa silages were conducted using high-performance liquid chromatography and high-throughput sequencing methods.ResultsAlfalfa was ensiled with additives (LF, CE, and LF+CE) or without additives for 60 days. All additives increased lactic acid and decreased pH values and ammonia-N contents compared to control. Among all treatments, the combined addition of LF and CE showed lowest pH (4.66) and ammonia-N (NH3-N, 0.57% DM) content, highest contents of lactic acid (LA, 10.51% DM), dry matter (DM, 22.54%) and crude protein (CP, 24.60% DM). Combined addition of LF and CE performed better in reducing neutral detergent fiber (NDF, 29.76% DM) and acid detergent fiber (ADF, 22.86% DM) contents than the addition of LF (33.71, 27.39% DM) or CE (32.07, 25.45% DM) alone. Moreover, the microbial analysis indicated that LF+CE treatments increased the abundance of desirableLactobacillusand inhibited the growth of detrimentalEnterobacterandClostridiain alfalfa silage.DiscussionCombined addition of FAE-producing LF and CE is more effective than treatments of LF or CE alone in improving fermentation quality and nutrition values of alfalfa silage. This is likely due to a synergistic effect of CE and FAE produced by LF on plant cell wall degradation, indicating that these additives promote each other to improve fiber degradation and silage fermentation. In conclusion, combined addition of FAE-producing LF and CE could be a feasible way to improve alfalfa silage quality.
Identification of crucial genes in abdominal aortic aneurysm by WGCNA
Authors: Chen Siliang , Yang Dan , Lei Chuxiang , Li Yuan , Sun Xiaoning , Chen Mengyin , Wu Xiao , Zheng Yuehong 
Published: October 2019

Background
Abdominal aortic aneurysm (AAA) is the full thickness dilation of the abdominal aorta. However, few effective medical therapies are available. Thus, elucidating the molecular mechanism of AAA pathogenesis and exploring the potential molecular target of medical therapies for AAA is of vital importance.

Methods
Three expression datasets (GSE7084, GSE47472 and GSE57691) were downloaded from the Gene Expression Omnibus (GEO). These datasets were merged and then normalized using the “sva” R package. Differential expressed gene (DEG) analysis and weighted gene co-expression network analysis (WGCNA) were conducted. We compared the co-expression patterns between AAA and normal conditions, and hub genes of each functional module were identified. DEGs were mapped to co-expression network under AAA condition and a DEG co-expression network was generated. Crucial genes were identified using molecular complex detection (MCODE) (a plugin in Cytoscape).

Results
In our study, 6 and 10 gene modules were detected for the AAA and normal conditions, respectively, while 143 DEGs were screened. Compared to the normal condition, genes associated with immune response, inflammation and muscle contraction were clustered in three gene modules respectively under the AAA condition; the hub genes of the three modules were MAP4K1, NFIB and HPK1, respectively. A DEG co-expression network with 102 nodes and 303 edges was identified, and a hub gene cluster with 10 genes from the DEG co-expression network was detected. YIPF6, RABGAP1, ANKRD6, GPD1L, PGRMC2, HIGD1A, GMDS, MGP, SLC25A4 and FAM129A were in the cluster. The expression levels of these 10 genes showed potential diagnostic value.

Conclusion
Based on WGCNA, we detected 6 modules under the AAA condition and 10 modules in the normal condition. Hub genes of each module and hub gene clusters of the DEG co-expression network were identified. These genes may act as potential targets for medical therapy and diagnostic biomarkers. Further studies are needed to elucidate the detailed biological function of these genes in the pathogenesis of AAA.
Socioeconomic and Behavioral Determinants That Permeate The Active Aging of Elderly People From a Community Living Center / Determinantes Socioeconômicos e Comportamentais Que Permeiam o Envelhecimento Ativo dos Idosos de Um Centro Comunitário de Convivência
Authors: Mira Bruna Corrêa , Rodrigues Ferreira Angela Maria , Ozela Claudia do Santos , Penha de Oliveira Santos Maria Izabel , Palmeira Iaci Proença , Dias da Silva Silvio Éder 
Published: October 2019

Objetivo: Descrever os determinantes socioeconômicos e comportamentais que permeiam o envelhecimento ativo dos participantes do grupo de convivência de uma Universidade da Terceira Idade. Método: Pesquisa descritiva, quantitativa, realizada a partir de um questionário, com 183 participantes de grupos de convivência de uma universidade pública de Belém/PA. Resultado: Dos 183 participantes, foi predominante 91% do gênero feminino, 71% com idades entre 60 e 70 anos; 51% apresentaram escolaridade superior a 8 anos; 68% residiam com seus familiares e 55% eram ativos economicamente. Observou-se que 99% referiu mudança após início da participação no grupo, 80% apresentou pelo menos uma comorbidade, 91% praticava atividades físicas e 73% usava racionalmente os medicamentos. Conclusão: Os determinantes socioeconômicos e comportamentais dos participantes do estudo apontam satisfação no convívio grupal, conhecimento, qualidade de vida e a promoção do envelhecimento ativo. Descritores: Envelhecimento; Comportamentos Saudáveis; Centros Comunitários Para Idosos.
Multiprofessional Care Towards the Crack-Related Hospital Admissions / O Cuidado Multiprofissional na Prevenção de Internações Relacionadas ao Uso de Crack
Authors: Nazaré Oliveira Eliany , Martins Moreira Roberta Magda , Da Silva Oliveira Lycélia , Dos Santos Olímpio Anny Caroline , De Souza Silva Rita Wigna , De Almeida Pereira Paulo Jorge 
Published: October 2019

Objetivo: Analisar a relação do cuidado multiprofissional ofertado em um Centro de Atenção Psicossocial para Álcool e Outras Drogas (CAPS AD) com o número de internação hospitalar relacionada diretamente com o consumo de drogas em usuários de crack. Métodos: Estudo documental com abordagem quantitativa, realizado com 213 prontuários de usuários de crack de um CAPS AD, referente ao perfil socioeconômico, atendimentos individuais, em grupo e internação hospitalar. As informações foram processadas no SPSS versão 22. Resultados: Observou-se predomínio de homens (76,5%), com idade entre 11 a 47 anos, solteiros (60,8%) que possuíam ensino fundamental incompleto (53,5%). Os atendimentos com maior significância estatística foram enfermagem, psiquiatria, clínica médica e técnico em enfermagem, bem como grupos de atividade física, arteterapia e prevenção de recaída. Conclusão: É necessário realizar assistência contínua e integral ao usuário de crack, com equipe multiprofissional atuando de forma complementar para minimizar as internações referentes ao consumo da droga.
The Nurse Approach Towards the Detection of Antecedent Signs and Symptoms of Sepsis in Patients at a Nursing Ward / O Enfermeiro na Detecção dos Sinais e Sintomas que Antecedem Sepse em Pacientes na Enfermaria
Authors: César Oliveira Simone , Taboas Corrêa Bruna , Nogueira Dodde Hanna , Lombardo Pereira Gicélia , Gerbassi Costa Aguiar Beatriz 
Published: October 2019

Objetivo: Descrever os sinais e sintomas que antecedem a sepse em pacientes internados na Clínica Médica de um Hospital Federal no Rio de Janeiro identificados pelo Enfermeiro; analisar como o Enfermeiro correlaciona os sinais e sintomas com a Sepsis-1, Sepsis-2 e Sepsis-3. Métodos: Estudo descritivo com abordagem quantitativa, a população foram 10 Enfermeiros em plantões diurnos na enfermaria da Clínica. A coleta de dados foi um questionário estruturado, abordando identificação dos sinais e sintomas que antecedem a sepse, englobando as características e particularidades da sepse. Resultados: Evidenciou-se que possuem entendimento sobre o conceito de sepse, entretanto apresentaram dificuldades em correlacionar alguns dos sinais e sintomas dos tipos de sepse. Conclusão: Ciente que a sepse é um problema de saúde e o Enfermeiro presta cuidado direto ao paciente, percebe-se a importância na identificação dos sinais e sintomas que a antecedem para oferecer assistência de qualidade e auxiliar na redução dos casos.Descritores: Enfermagem, Sepse, Sinais e sintomas, Síndrome de resposta inflamatória sistêmica.
The Difficult Daily Life of Heart Failure Bearing Patients / O difícil cotidiano dos pacientes com insuficiência cardíaca
Authors: Abreu e Souza Thereza Cristina Terra de Oliveira de , Correia Dayse Mary da Silva , Nascimento Daiana Cordeiro , Christovam Barbara Pompeu , Batista Deyse Conceição Santoro , Cavalcanti Ana Carla Dantas 
Published: October 2019

Objetivo: analisar as principais limitações relatadas por pacientes com insuficiência cardíaca. Método: análise secundária de um estudo exploratório, de abordagem qualitativa, realizado com 15 pacientes portadores de insuficiência cardíaca no período de março a julho de 2011, dentre os 167 em atendimento ambulatorial. A coleta de dados primários deu-se a partir de uma entrevista semi-estruturada, com posterior análise de Bardin, e para presente análise secundária foi utilizada como estratégia a interpretação retrospectiva. Resultados: predominância do sexo feminino (73,3%); ensino fundamental incompleto (80,2%); hipertensos (80,2%); dislipidêmicos (53,4%); diabéticos (33,3%). Quanto às limitações no cotidiano, destacam-se: deambulação prejudicada, padrão de sono precário, fadiga, dispneia, perda de autonomia, dependência de terceiros, padrão de sono precário, baixa frequência da atividade sexual. Conclusão: na insuficiência cardíaca, uma doença crônica, é fundamental que o enfermeiro busque por orientações e estratégias, as quais possam desenvolver a capacidade de autocuidado, prevenção e promoção da saúde.
Early diagnosis of skin cancer by ultrasound frequency analysis
Authors: Kia Shabnam , Setayeshi Saeed , Pouladian Majid , Ardehali Seyed Hossein 
Published: October 2019

AbstractThe diagnosis of cancer by modern computer tools, at the very first stages of the incident, is a very important issue that has involved many researchers. In the meantime, skin cancer is a great deal of research because many people are involved with it. The purpose of this paper is to introduce an innovative method based on tissue frequency analyzes to obtain the accurate and real‐time evaluation of skin cancers. According to the Biological resonance theory, body cells have natural and unique frequencies based on their biological fluctuations, which, if the structure, profile and cellular status change, its frequency also varies. This concept and theory is considered as the basis for analyzing skin tissue health in the proposed method. Reflected ultrasound waves from tissue have been processed and studied based on frequency analysis as a new method for early detection and diagnosis of accurate location and type of skin diseases. The developed algorithm was approved through 400 patients from CRED; its ability to evaluate benign and malignant skin lesions was shown (AUC = 0.959), with comparable clinical precision; as for the selected threshold, sensitivity, and specificity were 93.8% and 97.3%, respectively. Therefore, this method can detect skin malignancy with an accurate, noninvasive and real‐time procedure.
Signal and contrast to noise ratio evaluation of fluoroscopic loops for interventional fluoroscope quality control
Authors: Goode Allen R. , Snyder Carl , Snyder Angela , Collins Patricia , DeLorenzo Matthew , Lin Pei‐Jan 
Published: October 2019

AbstractModern fluoroscopes pose a challenge for the clinical physicist for annual testing and continued upkeep. These fluoroscopes are critical to providing care to patients for complex interventions, and continue to evolve in automated image quality adjustments. Few tools in software or hardware currently exist to assist the physicist or technologist in gauging fluoroscope constancy or readiness for procedures. Many modalities such as mammography, computed tomography or even magnetic resonance imaging are much more evolved with respect to testing or quality control. In this work we sought to provide simple reproducible tools and methods for spot evaluating or continued quality testing of interventional fluoroscopes.
High‐Throughput Combinatorial Optimizations of Perovskite Light‐Emitting Diodes Based on All‐Vacuum Deposition
Authors: Li Jinghui , Du Peipei , Li Shunran , Liu Jing , Zhu Menghua , Tan Zhifang , Hu Manchen , Luo Jiajun , Guo Deqiang , Ma Lin , Nie Zhaogang , Ma Ying , Gao Liang , Niu Guangda , Tang Jiang 
Published: October 2019

AbstractLight‐emitting diodes (LEDs) based on lead halide perovskites demonstrate outstanding optoelectronic properties and are strong competitors for display and lighting applications. While previous halide perovskite LEDs are mainly produced via solution processing, here an all‐vacuum processing method is employed to construct CsPbBr3 LEDs because vacuum processing exhibits high reliability and easy integration with existing OLED facilities for mass production. The high‐throughput combinatorial strategies are further adopted to study perovskite composition, annealing temperature, and functional layer thickness, thus significantly speeding up the optimization process. The best rigid device shows a current efficiency (CE) of 4.8 cd A−1 (EQE of 1.45%) at 2358 cd m−2, and best flexible device shows a CE of 4.16 cd A−1 (EQE of 1.37%) at 2012 cd m−2 with good bending tolerance. Moreover, by choosing NiOx as the hole‐injection layer, the CE is improved to 10.15 cd A−1 and EQE is improved to a record of 3.26% for perovskite LEDs produced by vacuum deposition. The time efficient combinatorial approaches can also be applied to optimize other perovskite LEDs.
Influence of strain and fermentation time on the production, composition, and properties of xanthan gum
Authors: Miranda Andrea Lobo , Costa Samantha Serra , Assis Denilson de Jesus , de Jesus Cristiane Santos , Guimarães Alaíse Gil , Druzian Janice Izabel 
Published: October 2019

ABSTRACTThe objective of this study was to monitor the influence of Xanthomonas spp. and fermentation time on the production, properties, and composition of xanthan gum, verifying how these variables affect the biopolymer synthesis and properties. Three strains were monitored for 168 h of fermentation. Every 24 h, a sample was collected to determine cell growth, gum production, and substrate consumption. Fourier transform infrared, X‐ray diffraction, rheology, molecular characterization, thermogravimetric analysis, sugars, and acids analyzes were performed to characterize the gums produced and to identify possible differences in these properties and composition. The results showed the influence of strain and the fermentation time in production (Pr645 = 0.047 g L−1 h−1, Pr290 = 0.039 g L−1 h−1, Pr1155 = 0.149 g L−1 h−1) and gum quality, since the viscosity was highest in the first 48 h for strains 290 (148.71 MPa s−1) and 645 (135.28 MPa s−1), while for Strain 1155, the maximum value identified for this property was 90.48 MPa s−1. These results are of practical importance, since they allow obtaining better quality xanthan, enabling reduction of production costs. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2020, 137, 48557.
Revisiting “the 1990s debutante”: Scholar‐led publishing and the prehistory of the open access movement
Authors: Moore Samuel A. 
Published: October 2019

AbstractThe movement for open access publishing (OA) is often said to have its roots in the scientific disciplines, having been popularized by scientific publishers and formalized through a range of top‐down policy interventions. But there is an often‐neglected prehistory of OA that can be found in the early DIY publishers of the late 1980s and early 1990s. Managed entirely by working academics, these journals published research in the humanities and social sciences and stand out for their unique set of motivations and practices. This article explores this separate lineage in the history of the OA movement through a critical‐theoretical analysis of the motivations and practices of the early scholar‐led publishers. Alongside showing the involvement of the humanities and social sciences in the formation of OA, the analysis reveals the importance that these journals placed on experimental practices, critique of commercial publishing, and the desire to reach new audiences. Understood in today's context, this research is significant for adding complexity to the history of OA, which policymakers, advocates, and publishing scholars should keep in mind as OA goes mainstream.
Parenting stress in caregivers of young children with ASD concerns prior to a formal diagnosis
Authors: DesChamps Trent D. , Ibañez Lisa V. , Edmunds Sarah R. , Dick Catherine C. , Stone Wendy L. 
Published: October 2019

Robust findings demonstrate that parents of children with autism spectrum disorder (ASD) experience high levels of parenting stress that are associated with negative outcomes for families. Although the majority of research on parenting stress has focused on parents of children with an existing diagnosis, parents of young children with concerns about ASD‐related behaviors also face many unique challenges before the time of diagnosis. However, no study to date has examined patterns of parenting stress among parents of children with ASD concerns prior to a formal ASD diagnosis. Therefore, the current study investigated longitudinal trajectories of parenting stress among parents of young children with ASD concerns compared to parents of children with non‐ASD developmental concerns (e.g., language delay), and parents of children with no developmental concerns. Known predictors of parenting stress were also examined. Results from multilevel model analyses revealed that parents of children with ASD concerns experienced consistently higher levels of parenting stress across early child development compared to parents of children with non‐ASD developmental concerns and those with no concerns. Additionally, parenting efficacy, psychological functioning, social satisfaction, and child social communication behaviors predicted levels of parenting stress for all parents. Autism Res 2020, 13: 82–92. © 2019 International Society for Autism Research, Wiley Periodicals, Inc.Lay SummaryThis study examined parenting stress across time among parents of young children with ASD concerns before receiving a diagnosis. Parents of children with ASD concerns reported consistently higher levels of parenting stress compared to parents of children with other developmental concerns and parents of children with no concerns. Also, ASD concerns predicted parenting stress in addition to other parent and child predictors of parenting stress. These findings highlight the need to better support families before an ASD diagnosis.
News in fusion
Authors: Lübke Stefanie , Löwer Andreas , Papendieken Michaela , Filiz Önel Susanne 
Published: October 2019

ZusammenfassungDie Bildung eines synzytialen Muskels beruht auf der Fähigkeit von einkernigen Myoblasten zu fusionieren. Wesentliche Prozesse während der Myoblastenfusion von Drosophila und Säugetieren sind dabei die Zellerkennung und Zelladhäsion, die Bildung eines Zug‐ und Druckkräfte generierenden Zentrums durch Polymerisation von monomerem zu F‐Aktin, die Bildung einer Fusionspore sowie die Vermischung des Zytoplasmas und die Integration der Myoblasten in die wachsende Myotube. In den letzten 20 Jahren wurden viele Schlüsselgene der Myoblastenfusion am Modellorganismus Drosophila melanogaster identifiziert. Keines dieser Gene kodiert für ein Fusogen. Im Gegensatz dazu wurden in den letzten Jahren bei Mäusen zwei Proteine identifiziert, die direkt an der Umstrukturierung der Plasmamembran während der Bildung einer Fusionspore beteiligt sind und die fusogene Eigenschaften besitzen. Diese Proteine können uns zukünftig helfen zu verstehen, was während der Myoblastenfusion an der Zellmembran passiert.
Zuckerbrot und Peitsche
Authors: Will Maria , Jerominek Markus 
Published: October 2019

ZusammenfassungPfeilwurzgewächse gehören zu den wenigen Beispielen für sehr schnelle pflanzliche Bewegungen. Diese kennt man unter anderem von fleischfressenden Pflanzen oder der Mimose. Das bekannteste Beispiel ist die Schließbewegung der Fangblätter von Venusfliegenfallen (Dionaea). Hier sind elektrische Impulse die Ursache für eine schnelle Reaktion auf den Reiz. Die Frage, wie das explosionsartige Einrollen des Griffels der tropischen Pfeilwurzgewächse zustande kommt, konnten bisherige Untersuchungen noch nicht zufriedenstellend beantworten. Elektrophysiologische Experimente helfen dabei, den Mechanismus hinter der Griffelbewegung besser zu verstehen.
Biomimetic Assembly of a Polydopamine Layer on Graphene as an Electron Gate for Fluorescent MicroRNA Detection in Living Cells
Authors: Li Xinyi , Wang Ting , Luo Yonglan , Asiri Abdullah M. , Chen Song , Sun Xuping 
Published: October 2019

AbstractA novel strategy was developed for microRNA detection based on the fluorescence quenching of polydopamine (PDA)‐coated reduced graphene oxide (RGO) nanosheets (RGO@PDA). Compared with graphene oxide (GO), the reduction of GO and modification of the surface of RGO by PDA not only improve the stability, dispersity, biocompatibility, and cellular uptake without degeneration of the unique electronic properties of graphene, but also add an electron gate for harvesting electrons, as well as enabling efficient and forward electron transfer to avoid unwanted electron transfer and realize highly sensitive miRNA detection; thus a lower detection limit can be achieved in this sensing system. Remarkably, nanoprobes consisting of RGO@PDA and fluorescein‐labeled single‐stranded DNA can naturally enter cancer cells without the aid of transfection agents, as well as resisting enzymatic lysis and showing almost no effect on the cell viability. More importantly, intense and time‐dependent fluorescence responses were observed from the important tumor marker microRNA‐21 (miR‐21) in living cells; thus suggesting that the proposed sensing platform shows great promise for applications in disease diagnosis and fundamental research into biochemistry.