bluuebunny/crossref_metadata_2025_split huggingface dataset:
Classification and identification the most important features of cervical cancer based on the expression of microRNA gene with the random forest (RF) algorithm
Authors: Aziz E A , Wibowo A , Wirawan P W 
Published: June 2019

Abstract
Cervical cancer is the leading cause of death women in the world and number one in Indonesia. An effort that can be done for this case is early detection, for example, an IVA test (visual inspection test with acetic acid). However, the IVA test is not able to indicate patients who have potential cancer before cancer’s physical characteristics are seen. Thus a new solution is needed for early detection of cervical cancer that can indicate patients who have potential cancer before cancer’s physical characteristics are seen. In recent years, various types of miRNA that play a role in cancer malignancies have been identified and can be used as non-invasive biomarkers for cancer diagnosis and monitoring. The use of classification based on miRNA gene expression is a solution for early detection, but the use of high accuracy classification algorithms is something that must be considered. Random Forest (RF) algorithm is the solution to these problems because better generalization performance and is less susceptible to overfitting. In this study also identified important features that are very influential in the classification process. The results showed that the Random Forest algorithm was able to have 100% accuracy for classification and most important features supporting the cancer were miR-549c-5p, miR-183 and miR-515-5p.
The effect of coconut water and tofu wastewater as nitrogen source on the production of alkali protease from Aspergillus flavus DUCC K225
Authors: Rukmi I , Pujiyanto S , Mulyani N S , Faidah N , Ayu L 
Published: June 2019

Abstract
Protease is one of important industrial enzyme produced by microorganisms, including fungi. Aspergillus flavus DUCC K225 is an indigenous mold isolated from lime soil Madura island, has been verified as potential producing protease at pH 8. The production of alkaline protease by A. flavus DUCC K225 was studied under submerged fermentation. Molasse and glucose were used to replace sucrose as carbon source. The effect of medium components was observed on the 7th day’s incubation, by measuring biomass, protease activity, protein content and protease specific activity at pH 8.5. The examination were done for crude enzyme, 0-20%, 20-40%, 40-60%, 60-80% and 80-100% ammonium phosphate fractionation. The results showed that highest biomass found in glucose medium (14,27 g/L); the highest protease specific activity was found in 60-80% ammonium sulfate fractionation of molasses medium (120,17 U/ml) and 9,4 purification fold.
Bioindicator for environmental water quality based on saprobic and diversity indices of planktonic microalgae: a study case at Rawapening lake, Semarang district, Central Java, Indonesia
Authors: Hariyati R , Putro S P 
Published: June 2019

Abstract
Over the last twenty years, the aquatic environment of Rawapening Lake has been negatively impacted by increasing rates of sedimentation and rising levels of soluble organic materials. The uncontrolled growth rate of hyacinth and other aquatic plants, and the rapidly increased implementation of traditional fish cages are believed to be the main contributing factors, resulting in degraded water quality. Dynamic structure of phytoplankton can be used as a bioindicator. This study is aimed at understanding the phytoplankton community in Rawapening Lake and assessing the water quality based on saprobic and diversity indices. A purposive random sampling method was used to determine four sampling locations. The data were analyzed with the saprobic index, Shanon-Wiener diversity index, and domination index. The result of the study shows that a substantial amount of phytoplankton were dominated by the genus Melosira sp., Aulacoseria granulosa, Oscillatoria sp., and Synedra sp., with low to moderate diversity index values. The quality of the Rawapening Lake water environment based on the saprobic index value was categorized as β meso-Oligosaprobic, indicating very mild to light pollution.
Analysis of glycemic index of “Gula Semut” through blood glucose level test
Authors: Winarni S , Arifan F , Broto RTD. W , Fuadi A , Ramadhan R 
Published: June 2019

Abstract
The glycemic index is a measure developed to classify carbohydrate foods based on their physiological effects on blood glucose levels. The purpose of this study was to distinguish glycemic index of ant sugar compared to comparator (carbohydrate 50 gram). Respondents involved in the glycemic index test were 5 persons. Prior to treatment (fresh bread and “gula semut”) respondents fasting at least 10 hours. In the first stage respondents were given 50 grams of fresh bread measured blood sugar levels at 0 minutes (fasting), 15, 30, 45, 60, 90, and 120 minutes post giving bread. Respondents were rested for 3 days without heavy activity. The second stage of the respondents was given 50 grams of ant sugar and measured blood sugar level of 0 minutes (fasting), 15, 30, 45, 60, 90, and 120 minutes post giving “Gula semut”. Blood glucose measurement using finger-prick capillary blood samples method. Calculation of glycemic index to bread and “gula semut" obtained through calculation of area under the curve. The total area under the curve on the supply of bread 35.75% while the total area under the curve on the supply of “gula semut” 14.10%. The glycemic index of “gula semut” is low (<55%). Low glycemic index foods improve slowly blood sugar levels. So the “gula semut” does not raise the blood glucose levels quickly.
Ecotourism development strategy at minapolitan area of Menayu Village, Magelang District, Central Java, Indonesia
Authors: Putro S P , Wulandari A , Muhammad F 
Published: June 2019

Abstract
Menayu Village is one of the villages in the Muntilan District, Magelang Regency, Central Java which is one of the minapolitan areas based on Magelang Regent’s Decree (SK) No. 188.45 / 347 / KEP / 29/2011. Minapolitan area is a part of the area that has the main function of the economy which consists of production centers, processing, marketing of fishery commodities, services, and other supporting activities. The purpose of this study was to determine internal and external factors that support the development of ecotourism in the Minapolitan area and the appropriate ecotourism development strategies to be able to develop Menayu as the minapolitan area. SWOT analysis was used, involving internal strategy factors (IFAS) and external strategy factors (EFAS). The results showed that IFAS factors that support ecotourism development in the Minapolitan area include rural natural beauty, topography, diversity of plankton and fish in the area of fish farming, community support and the farm of red arowana fish (Schleropagus formosus), while weakness factors for tourism were infrastructure and lack of public knowledge. Supporting EFAS factors were the potential aquaculture sector, local government support, and stakeholders, the presence of red arowana fish (Schleropagus formosus) which are included in the IUCN category for endangered species. While the obstacles were unoptimal stakeholder support and the lack of transportation to the area from the district center. Priority strategies that can be applied based on the SWOT analysis are protection and conservation of ecotourism potential (conservation of aquatic resources and red arowana fish and the optimization of biological potential as a tourist attraction, as well as enhancing ecotourism support facilities.
Morphologic characteristics and population density of Teredo Navalis L in mangrove forest area, Wailukum East Halmahera Regency
Authors: Sinyo Y. , Anggoro S. , Soeprobawati T. R. 
Published: June 2019

Abstract
This study aimed to analyze the population density and distribution patterns of Teredo Navalis L in the mangrove forest area of Wailukum Maba, East Halmahera Regency. A survey method with deskriptive analysis used in this research, while the reseacrh location was determined using a purposive method. The research location is 100 meters long and 50 meters wide, making it 5,000 m2. The research location was divided into 4 stations. Station I in the north, station II at the center, station III in the east and station IV in the west. At each station, five transect lines were made using a rope and 5 plots were placed alternately with a size of 10x10 meters. The results showed that there were three morphological characteristics of T. navalis L. These carakteristics divided in mangrove logs based on body measurements 0f 60 cm long, 30 cm long and 15 cm long. The population density of T. navalis L at the four stations varied between 1-2 ind / m2. the highest population density was measured at station II (2 ind/m2) and the lowest was measured at station III (1 ind/m2).
Applications of mycorrhiza on potato growth and productivity
Authors: Susiana Purwantisari , Rukmi Isworo , Jannah Siti Nur 
Published: June 2019

Abstract
Mycorrhiza is a root plant symbiont functioning to increase mineral and nutrients absorption. Mycorrhiza fertilization to potatoes has to be monitoredin order to observethe effects of mycorrhiza on the growth of potatoes. Therefore, theaim of thisstudy was to analyze the effect of mycorrhiza on potato growth and production. This field research was conducted in Kledung village, Temanggung (1,138 masl) from 25 March to 25 June 2018. The method used was separated block design with 4 treatments and 20 replications in each plot. The first plot (P1) was the plot without treatment; the second plot (P2), positive control, was the plot treated with chemical fertilizer;the third plot (P3) was plot treated with organic fertilizer Bokasi and Trichoderma; and the fourth plot (P4) was fertilized using mycorrhiza. The data related to growth and production were analyzed using ANOVA and Tukey’s test; while, to determine the best type of fertilizer among treatments t-testwas used. The results showed that mycorrhizal application significantly increased height (20.12 cm p ≤ 0.05), the number of leaf (32.47, p ≤ 0.05), and potato production (213.70 gr). It can be concluded that the use of mycorrhiza significantly increased the growth and production of potato.
The autoalelopathic potential of the Siam weed (Chromolaena odorata l.) leaf extract as a natural herbicide
Authors: Ziadaturrif’ah D , Darmanti S , Budihastuti R 
Published: June 2019

Abstract
The siam weed (Chromolaena odorata L.) has allelochemical compounds capable in inhibiting the growth of surrounding plants so that it has the potential as a natural herbicide to control various weeds including autoallelopathic weeds. This study aims to examine the effect of siam weed leaf extract at different concentrations on germination and initial growth of the Siam weed seed. The study used a Completely Randomized Design of one factor in the form of extract concentrations of 0%, 5%, 10%, 15%, and 20%. Germination method was carried out on petri dish and observations are carried out every 24 hours, and the treatment was stopped after 100% germination occurred at least in one petri dish. Each treatment was done with five replications — parameters measured as a percentage of germination and germination rate, initial germination including hypocotyl length, radicula length, and fresh weight. Data were analyzed by Analysis of Variance (ANOVA) and continued with Duncan’s Multiple Range Test (DMRT) at 95% confidence level. The results of this study showed that the Siam weed extract inhibited germination and initial growth of the Siam weed seed sprout, the higher concentration of the Siam weed extract the highest, inhibition of germination and initial growth of the Siam weed seed.
Distribution of total suspended solids (TSS) and chlorophyll-a in Kendari Bay, Southeast Sulawesi
Authors: Fanela M A P , Takarina N D , Supriatna  
Published: June 2019

Abstract
Kendari Bay is receiving direct pressure from land clearing. This activity leads to sedimentation to the estuarine area. The sedimentation rate in Kendari Bay reaches up to 143,147 m3/year. The high amount of total suspended solids will impede light penetration. As a consequence, it will decrease the rate of photosynthesis, thus decreasing chlorophyll-a content. This research was aimed to understand the relationship between distribution total suspended solid (TSS) and chlorophyll-a concentration in Kendari Bay. Water sampling was done between January 21 and February 6, 2018. TSS and chlorophyll-a were measured in the laboratory. Data of TSS and chlorophyll-a from 2010 to 2018 were downloaded from Landsat 5 TM and Landsat 8 OLI. ENVI 5.1 and ArcGIS 10.1 software was used to visualize the distribution into a map. The relationship between two parameters was analyzed using linear regression. The TSS showed a linear increase from 2010 to 2018 with a concentration higher than 50 mg.l−1. The correlation between TSS and chlorophyll–a showed a negative correlation (0.6376) which indicated that the higher TSS, the lower the chlorophyll-a.
Effect supplementation of turmeric powder (Curcuma longa L.) on histomorphometric duodenal female Melopsittacus undulates
Authors: Baehaqi I , Saraswati T R , Yuniwarti E Y W 
Published: June 2019

Abstract
Turmeric powder supplements contain curcumin and essential oils that can increase parakeet production. The aims study to analyze effect supplementation turmeric powder (Curcuma longa L.) on duodenal female parakeets (Melopsittacus undulates). This research used ten female parakeets aged four months. Research used a randomized design complete with two treatments and five replications. The treatment was P1 as control and P2 with supplement turmeric powder dose 35 mg/bird/day for 30 days. The result using t-test showed that effect turmeric powder did not make significant (p> 0.05) on body weight, feed consumption, gastrointestinal length, and duodenal histomorphometry (villi height, villi width, crypt depth, and mucosal muscular thickness). The conclusion is supplementation turmeric powder on female parakeets (Melopsittacus undulates) not effect on body weight, feed consumption, gastrointestinal length, and duodenal histomorphometry (villi height, villi width, crypt depth, and mucosal muscular thickness). Supplementation turmeric powder dose 35 mg/bird/day not positive impact on the digestive system of parakeets.
Characterization phosphate-solubilizing marine actinobacteria associated with Sargassum Sp from Menjangan kecil island, Indonesia
Authors: Lunggani A T , Suprihadi A. 
Published: June 2019

Abstract
Phosphorus plays a crucial significant role in all life forms. Microbes have the ability to convert several forms of phosphorus into dissolved forms, so that they can also be used in the phosphorus cycle process. The aim of this research were to explore, screen, phenetic and molecular identify the phosphobacteria naturally colonized and associated with Sargassum sp. from the Menjangan Kecil Island, Karimunjawa. Total of 23 bacterial isolates associated with Sargassum sp. were screened. All isolates obtained were tested for their ability in phosphate dissolution activity in Pikovskaya agar medium. Three different isolates showed the largest dissolution activity around bacterial colonies selected for quantitative estimation of phosphate solubilization. The next screening was to choose one candidate isolate based on its highest ability in dissolving phosphate and then identifying its identity based on phenotypic character and 16S rRNA gene. Quantitative estimation of phosphate solubilization PSB isolates MBS3R was 149,32 μg P ml-1 was analyzed for 120 hours of incubation at room temperature. MBS3R isolate is a strain of Microbacterium sp, which is taxonomically included in the class of Actinobacteria, genus Microbacterium.
Application of cinnamon and gotu kola supplements for increasing quail hematological status (Coturnixcoturnix-australica)
Authors: Mas’adah S M , Sunarno  , Djaelani M A 
Published: June 2019

Abstract
Cinnamon and gotu kola are herbal plants that are rich in polyphenol-type antioxidants. This type of antioxidant has been known to have an important role in stimulating an increase in biological function, especially hematological status. Quail with increased haematological status can be known from hemoglobin levels and erythrocyte counts per mm3 of blood. The aim of this study was to analyze the hematological status of quail Australian strains by adding cinnamon and gotu kola leaves supplements with variable hemoglobin levels, erythrocyte levels, and body weight supporting variables. The study design used a Completely Randomized Design consisting of 8 treatments with 3 replications. The treatment given includes control, feed supplemented with cinnamon bark powder 5% or 10%, supplementation of gotu kola leaf flour 5% or 10%, combination of cinnamon powder flour supplement with gotu kola (5%: 5%, 5%: 10 % or 10%: 5%). The results of the study were analyzed by Analysis of Variance (ANOVA) which continued with Duncan’s Test at a significance level of 5%. The results showed that the supplement of cinnamon bark and gotu kola leaves in feed gave a significant effect on body weight, hemoglobin levels, and the number of erythrocytes (P <0.05). A combination of gotu kola cinnamon-bark supplement with a ratio of 5%: 10% gives an effect on increasing hemoglobin and erythrocyte levels with a higher value than other treatments, which is equal to 13 g / % and 2,588,000 per mm3. Quail body weight in each treatment was not significantly different. The conclusion of this study is the addition of combination combinations of gotu kola cinnamon flour with a ratio of 5%: 10% can improve the hematological status of quails, so that the composition of this combination of ingredients can be used to increase the productivity of Australian strains quail laying.
Effect of combination explant difference leaf part and concentration of active charcoal on callus initiation mangrove (Rhizophora Apiculata BI) by in-vitro
Authors: Fitriana D , Prihastanti E , Nurchayati Y , Hastuti R B 
Published: June 2019

Abstract

Rhizophora Apiculata BI is one of the mangroves that has many benefits, but to cultivate naturally takes a long time. The alternative to such problems is culture cultivation in vitro. One stage of in vitro culture is through callus formation. Initiation of callus in mangrove plants is inhibited because explant release phenol compound that causes browning. Thus, it takes explant and charcoal active in a medium that can support growth explant. The purpose of this research is to study the effect of the combination of leaf explant and different activated charcoal concentration on callus initiation, to analyze the color and texture of callus produced, and to study the effect of the combination of leaf explant and different concentration of activated charcoal on the growth of explant. The basal medium used was Murashige and Skoog (MS) formula with the addition of NAA 1 mg/L and BAP 0.3 mg/L, and activated charcoal at concentration 4, 12, 20 g/L as the medium treatments. A different section of explant between base and tip of leaves combined with active charcoal were grown in MS medium. Each treatment was conducted three replications. The results showed that the combination of explant section, i.e. base of the leaf as well as activated charcoal with a concentration of 12 g / L could induce callus formation. The color and texture of callus were brownish yellow and compact. The best growth (0.1685 g) of callus was obtained at the leaf tip section combined with 4 g / L activated charcoal.
The Structure of Plankton as An Environmental Indicator for Water Management in Upper Part of Rawapening Lake, Semarang Regency, Indonesia
Authors: Hidayat J W , Hastuti R B , Hadi M , Yulianto G 
Published: June 2019

Abstract
The quality of Rawapening waters is constantly changing over time. The bloom growth of water hyacinth, fishery aquaculture activities and environmental pressures are getting heavier as expressed as eutrophication. This will affect in plankton composition. Plankton can provide an indication of changes and environmental quality, especially of its biotic structure and biodiversity. The upper part of Rawapening lake, similar to other part of the lake, is enriched with semi and aquatic plant. The purpose of this research is to analyze the condition of plankton currently, so that it can understand the change of water quality to support lake and fishery managements. The study was conducted in June 2018 on the upper parts of Rawapening Lake, particularly the southern side where many spring waters appear. Plankton collection was done by sampling using plankton net no 25. Data analysis was conducted descriptively and semi-quantitative using community structure including diversity, evenness and domination indices. The results showed that common plankton species found were Melosira sp, Synedra sp and Chlorella sp. Aulacosiera sp is quite abundant in all five stations reaching a range of 34-153 individuals / lt. In term of ecosystem stability, the waters are qualified as small to moderate stability conditions (H index :1.04 - 2.18) The species abundance tend to be small, especially in the spring station, which is associated with low water nutrients from springs. Aulacosiera sp is still dominate the plankton component for some station. The species which previously experienced a high domination, especially the Aulacoseir
a spp, change into a minority. This condition is mainly related to the changing physical condition of environmental. This, at a glance shows that the former water source area is still in good quality at this time experiencing enrichment. This is related to the increasing fishery practices of floating cage net of cultivation area approaching the spring water areas. The limitation and regulation in this area are urgently implemented and enforced, because clean water supply is important for the supply and circulation, which is not only for culture practice but also the lake in general.
Analysis of Pb residues on seaweed gracillaria spp in Randusangan District, Brebes, Central Java, Indonesia
Authors: Madusari B D , Hidayat J W , Permatasari M N 
Published: June 2019

Abstract
Heavy metal content in water always changes depending on lithology and disposal of contaminated waste. Heavy metals are difficult to decompose in water and are easily bound in sediment so that the more sediment accumulation, the greater the heavy metal content. Heavy metals have toxic effects on humans and other livings, accumulate in the body of an organism and stay for a long period. This compound can lead to a decrease in water quality which will affect the fishery and in pond cultivation. One of the important heavy metal pollutants is lead (Pb), which is usually came from many sources. The purpose of this study was to determine the concentration of lead in seaweed, pond water, and sediment. This research was conducted in March 2018 from 6 pond stations. This research was conducted using descriptive and statistic analysis. Analysis of Pb compound is done using AAS (Atomic Absorption Spectrophotometry). The results of the analysis of Pb content in seaweed ranged between 0.0163-0.1377 mg / kg. Pb content in pond water range from 0.0087 -0.0137 mg / lt and the Pb content in sediment range between 1.2703-2.3594 mg / lt. Based on the analysis, the Pb content is most abundant in sediments where the Pb content has exceeded the threshold value (SNI) of 0.3 mg/kg. Pb content on seaweed and pond water are still within the standard value of Pb metal contamination (BPOMRI No. 23 of 2013) which is 0.20 mg/kg. The relationship between Pb content in seaweed to the water is categorized as a weak (R = 0.073), it is similar to the seaweed with sediment (R = 0,28).