bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multidisciplinary studies on a sick-leader syndrome-associated mass stranding of sperm whales (Physeter macrocephalus) along the Adriatic coast of Italy
Authors: Mazzariol Sandro , Centelleghe Cinzia , Cozzi Bruno , Povinelli Michele , Marcer Federica , Ferri Nicola , Di Francesco Gabriella , Badagliacca Pietro , Profeta Francesca , Olivieri Vincenzo , Guccione Sergio , Cocumelli Cristiano , Terracciano Giuliana , Troiano Pasquale , Beverelli Matteo , Garibaldi Fulvio , Podestà Michela , Marsili Letizia , Fossi Maria Cristina , Mattiucci Simonetta , Cipriani Paolo , De Nurra Daniele , Zaccaroni Annalisa , Rubini Silva , Berto Daniela , de Quiros Yara Beraldo , Fernandez Antonio , Morell Maria , Giorda Federica , Pautasso Alessandra , Modesto Paola , Casalone Cristina , Di Guardo Giovanni 
Published: July 2018

AbstractMass strandings of sperm whales (Physeter macrocephalus) are rare in the Mediterranean Sea. Nevertheless, in 2014 a pod of 7 specimens stranded alive along the Italian coast of the Central Adriatic Sea: 3 individuals died on the beach after a few hours due to internal damages induced by prolonged recumbency; the remaining 4 whales were refloated after great efforts. All the dead animals were genetically related females; one was pregnant. All the animals were infected by dolphin morbillivirus (DMV) and the pregnant whale was also affected by a severe nephropathy due to a large kidney stone. Other analyses ruled out other possible relevant factors related to weather conditions or human activities. The results of multidisciplinarypost-mortemanalyses revealed that the 7 sperm whales entered the Adriatic Sea encountering adverse weather conditions and then kept heading northward following the pregnant but sick leader of the pod, thereby reaching the stranding site. DMV infection most likely played a crucial role in impairing the health condition and orientation abilities of the whales. They did not steer back towards deeper waters, but eventually stranded along the Central Adriatic Sea coastline, a real trap for sperm whales.
Transcriptome analysis based detection of Plasmodium falciparum development in Anopheles stephensi mosquitoes
Authors: Oakley Miranda S. , Verma Nitin , Myers Timothy G. , Zheng Hong , Locke Emily , Morin Merribeth J. , Tripathi Abhai K. , Mlambo Godfree , Kumar Sanjai 
Published: July 2018

AbstractThe Plasmodium life cycle within the mosquito involves the gamete, zygote, motile ookinete, and the oocyst stage that supports sporogony and sporozoite formation. We mapped the P. falciparum transcriptome as the parasite progresses through the oocyst stage of development on days 2, 4, 6, and 8 post-P. falciparum infectious blood meal. Through these genomic studies, we identified 212 novel transmission stage biomarkers including genes that are developmentally expressed at a single time point and genes that are pan-developmentally expressed at all four time points in P. falciparum oocysts. Validation of a small subset of genes at the transcriptional and translational level resulted in identification of a signature of genes/proteins that can detect parasites within the mosquito as early as day 2 post-infectious blood meal and can be used to distinguish early versus late stage P. falciparum oocyst development in the mosquito. Currently, circumsporozoite protein (CSP), which is detectable only after day 7 post-infection, is the only marker used for detection of P. falciparum infection in mosquitoes. Our results open the prospect to develop a non-CSP based detection assay for assessment of P. falciparum infection in mosquitoes and evaluate the effect of intervention measures on malaria transmission in an endemic setting.
The Metabolomic Bioenergetic Signature of Opa1-Disrupted Mouse Embryonic Fibroblasts Highlights Aspartate Deficiency
Authors: Bocca Cinzia , Kane Mariame Selma , Veyrat-Durebex Charlotte , Chupin Stéphanie , Alban Jennifer , Kouassi Nzoughet Judith , Le Mao Morgane , Chao de la Barca Juan Manuel , Amati-Bonneau Patrizia , Bonneau Dominique , Procaccio Vincent , Lenaers Guy , Simard Gilles , Chevrollier Arnaud , Reynier Pascal 
Published: July 2018

AbstractOPA1 (Optic Atrophy 1) is a multi-isoform dynamin GTPase involved in the regulation of mitochondrial fusion and organization of the cristae structure of the mitochondrial inner membrane. Pathogenic OPA1 variants lead to a large spectrum of disorders associated with visual impairment due to optic nerve neuropathy. The aim of this study was to investigate the metabolomic consequences of complete OPA1 disruption in Opa1−/− mouse embryonic fibroblasts (MEFs) compared to their Opa1+/+ counterparts. Our non-targeted metabolomics approach revealed significant modifications of the concentration of several mitochondrial substrates, i.e. a decrease of aspartate, glutamate and α-ketoglutaric acid, and an increase of asparagine, glutamine and adenosine-5′-monophosphate, all related to aspartate metabolism. The signature further highlighted the altered metabolism of nucleotides and NAD together with deficient mitochondrial bioenergetics, reflected by the decrease of creatine/creatine phosphate and pantothenic acid, and the increase in pyruvate and glutathione. Interestingly, we recently reported significant variations of five of these molecules, including aspartate and glutamate, in the plasma of individuals carrying pathogenic OPA1 variants. Our findings show that the disruption of OPA1 leads to a remodelling of bioenergetic pathways with the central role being played by aspartate and related metabolites.
Livestock trade network: potential for disease transmission and implications for risk-based surveillance on the island of Mayotte
Authors: Kim Younjung , Dommergues Laure , M’sa Ali Ben , Mérot Philippe , Cardinale Eric , Edmunds John , Pfeiffer Dirk , Fournié Guillaume , Métras Raphaëlle 
Published: July 2018

AbstractThe island of Mayotte is a department of France, an outermost region of the European Union located in the Indian Ocean between Madagascar and the coast of Eastern Africa. Due to its close connection to the African mainland and neighbouring islands, the island is under constant threat of introduction of infectious diseases of both human and animal origin. Here, using social network analysis and mathematical modelling, we assessed potential implications of livestock movements between communes in Mayotte for risk-based surveillance. Our analyses showed that communes in the central region of Mayotte acted as a hub in the livestock movement network. The majority of livestock movements occurred between communes in the central region and from communes in the central region to those in the outer region. Also, communes in the central region were more likely to be infected earlier than those in the outer region when the spread of an exotic infectious disease was simulated on the livestock movement network. The findings of this study, therefore, suggest that communes in the central region would play a major role in the spread of infectious diseases via livestock movements, which needs to be considered in the design of risk-based surveillance systems in Mayotte.
Astrocytic modulation of cortical oscillations
Authors: Bellot-Saez Alba , Cohen Greg , van Schaik André , Ooi Lezanne , W Morley John , Buskila Yossi 
Published: July 2018

AbstractBrain waves are rhythmic voltage oscillations emerging from the synchronization of individual neurons into a neuronal network. These oscillations range from slow to fast fluctuations, and are classified by power and frequency band, with different frequency bands being associated with specific behaviours. It has been postulated that at least ten distinct mechanisms are required to cover the frequency range of neural oscillations, however the mechanisms that gear the transition between distinct oscillatory frequencies are unknown. In this study, we have used electrophysiological recordings to explore the involvement of astrocytic K+clearance processes in modulating neural oscillations at both network and cellular levels. Our results indicate that impairment of astrocytic K+clearance capabilities, either through blockade of K+uptake or astrocytic connectivity, enhance network excitability and form high power network oscillations over a wide range of frequencies. At the cellular level, local increases in extracellular K+results in modulation of the oscillatory behaviour of individual neurons, which underlies the network behaviour. Since astrocytes are central for maintaining K+homeostasis, our study suggests that modulation of their inherent capabilities to clear K+from the extracellular milieu is a potential mechanism to optimise neural resonance behaviour and thus tune neural oscillations.
Histone deacetylase inhibitor, CG200745 attenuates renal fibrosis in obstructive kidney disease
Authors: Choi Hong Sang , Song Ji Hong , Kim In Jin , Joo Soo Yeon , Eom Gwang Hyeon , Kim Inkyeom , Cha Hyunju , Cho Joong Myung , Ma Seong Kwon , Kim Soo Wan , Bae Eun Hui 
Published: July 2018

AbstractTubulointerstitial fibrosis is a common feature of kidney disease. Histone deacetylase (HDAC) inhibitors have been reported to attenuate renal fibrosis progression. Here, we investigated the effect of CG200745, a novel HDAC inhibitor, on renal fibrosis development in a mouse model of unilateral ureteral obstruction (UUO). To examine the effects of CG200745 on renal fibrosis in UUO, C57BL/6 J male mice were divided into three groups: control, UUO, and CG200745 (30 mg/kg/day)-treated UUO groups. CG 200745 was administered through drinking water for 1 week. Human proximal tubular epithelial (HK-2) cells were also treated with CG200745 (10 µM) with or without TGF-β (2 ng/mL). Seven days after UUO, plasma creatinine did not differ among the groups. However, plasma neutrophil gelatinase-associated lipocalin (NGAL) levels were markedly increased in the UUO group, which were attenuated by CG200745 treatment. UUO kidneys developed marked fibrosis as indicated by collagen deposition and increased α-smooth muscle actin (SMA) and fibronectin expression. CG200745 treatment attenuated these fibrotic responses and suppressed UUO-induced production of transforming growth factor-beta1 (TGF-β) and phosphorylation of Smad-2/3. CG200745 treatment also attenuated UUO-induced inflammation as indicated by the expression of inflammatory markers. Furthermore, CG200745 attenuated phosphorylation of p38 mitogen-activated protein kinase in UUO kidneys. In HK-2 cells, TGF-β induced the expression of α-SMA and fibronectin, which were attenuated by CG200745 cotreatment. These results demonstrate that CG200745, a novel HDAC inhibitor, has a renoprotective effect by suppressing renal fibrosis and inflammation in a UUO mouse model.
RDM1 plays an oncogenic role in human lung adenocarcinoma cells
Authors: Tong Lu , Liu Jian , Yan Wangjun , Cao Wenjiao , Shen Shihui , Li Kun , Li Lei , Niu Guoping 
Published: July 2018

AbstractRAD52 motif containing 1 (RDM1) is involved in DNA damage repair pathway and RDM1−/− cells increase sensitivity to cisplatin, a common chemotherapy drug. Lung cancer is the leading cause of cancer death worldwide. However, the role of RDM1 in lung cancer is unknown. Here, we find that the mRNA and protein expression levels of RDM1 are significantly increased in human lung tumors, especially in lung adenocarcinoma. The lung adenocarcinoma patients with higher mRNA expression of RDM1 show the worse clinical outcomes. Knockdown of RDM1 in lung adenocarcinoma cells reduces cell proliferation and promotes apoptosis, consistent with the role RDM1 in the overexpression experiments. Xenograft mouse model shows stable knockdown of RDM1 significantly inhibits lung adenocarcinoma tumor growth. These in vitro and in vivo results conclude that RDM1 plays an oncogenic role in human lung adenocarcinoma. Interestingly, P53/RAD51/RAD52 can be regulated by RDM1, and the negative regulation of P53 by RDM1 may be one of major mechanisms for RDM1 to accomplish its oncogenic functions in lung adenocarcinoma. Therefore, RDM1 may be a new target for the treatment of lung adenocarcinoma.
Blocking ion channels induced by antifungal lipopeptide syringomycin E with amide-linked local anesthetics
Authors: Zakharova Anastasiia A. , Efimova Svetlana S. , Schagina Ludmila V. , Malev Valery V. , Ostroumova Olga S. 
Published: July 2018

AbstractThe effects of the amide-linked (lidocaine (LDC), mepivacaine (MPV), prilocaine (PLC)) and ester-bound local anesthetics (benzocaine (BZC), procaine (PRC), and tetracaine (TTC)) on the pore-forming activity of the antifungal lipopeptide syringomycin E (SRE) in lipid bilayers were studied. Independently on electrolyte concentration in the membrane bathing solution the observed changes in conductance of SRE channels agreed with the altered membrane dipole potential under the action of ester-bound local anesthetics. Effects of aminoamides in diluted and concentrated solutions were completely different. At 0.1 M KCl (pH 7.4) the effects of amide-linked anesthetics were in accordance with changes in the membrane surface potential, while at 2 M KCl aminoamides blocked ion passage through the SRE channels, leading to sharp reductions in pore conductance at negative voltages and 100-fold decreases in the channel lifetimes. The effects were not practically influenced by the membrane lipid composition. The interaction cooperativity implied the existence of specific binding sites for amide-bound anesthetics in SRE channels.
Trace mono-atomically dispersed rhodium on zeolite-supported cobalt catalyst for the efficient methane oxidation
Authors: Hou Yuhui , Nagamatsu Shinichi , Asakura Kiyotaka , Fukuoka Atsushi , Kobayashi Hirokazu 
Published: July 2018

AbstractThe partial oxidation of methane is a promising method for the efficient production of syngas. To implement this process using common stainless steel reactors, an inexpensive catalyst that functions at 650 °C or below is necessary. However, base metal catalysts typically require much higher temperatures, and they are deactivated by re-oxidation and coke formation. Here we report that modification of a zeolite-supported 3 wt% cobalt catalyst with a trace amount of mono-atomically dispersed rhodium (0.005 wt%) dramatically improves catalytic performance and durability. Cobalt/mordenite is nearly inactive due to the oxidation of cobalt, but the catalyst modified with rhodium continuously gives 85–86% methane conversion and 90–91% CO selectivity with an H2/CO ratio of 2.0 without serious coking at 650 °C. During the reaction, mono-atomically dispersed rhodium converts cobalt oxide to Co0 active species via hydrogen spillover. Use of the zeolite support is key to the high catalytic performance.
Impacts of radiative accelerations on solar-like oscillating main-sequence stars
Authors: Deal M. , Alecian G. , Lebreton Y. , Goupil M. J. , Marques J. P. , LeBlanc F. , Morel P. , Pichon B. 
Published: July 2018

Context. Chemical element transport processes are among the crucial physical processes needed for precise stellar modelling. Atomic diffusion by gravitational settling is usually taken into account, and is essential for helioseismic studies. On the other hand, radiative accelerations are rarely accounted for, act differently on the various chemical elements, and can strongly counteract gravity in some stellar mass domains. The resulting variations in the abundance profiles may significantly affect the structure of the star.Aims. The aim of this study is to determine whether radiative accelerations impact the structure of solar-like oscillating main-sequence stars observed by asteroseismic space missions.Methods. We implemented the calculation of radiative accelerations operating on C, N, O, Ne, Na, Mg, Al, Si, S, Ca, and Fe in the CESTAM code using the single-valued parameter method. We built and compared several grids of stellar models including gravitational settling, some with and others without radiative accelerations. We considered masses in the range [0.9, 1.5]M⊙and three values of the metallicity around the solar value. For each metallicity we determined the mass range where differences between models due to radiative accelerations exceed the uncertainties of global seismic parameters of theKeplerLegacy sample or expected for PLATO observations.Results. We found that radiative accelerations may not be neglected for stellar masses higher than 1.1M⊙at solar metallicity. The difference in age due to their inclusion in models can reach 9% for the more massive stars of our grids. We estimated that the percentage of the PLATO core program stars whose modelling would require radiative accelerations ranges between 33% and 58% depending on the precision of the seismic data.Conclusions. We conclude that in the context ofKepler, TESS, and PLATO missions which provide (or will provide) high-quality seismic data, radiative accelerations can have a significant effect when properly inferring the properties of solar-like oscillators. This is particularly important for age inferences. However, the net effect for each individual star results from the competition between atomic diffusion including radiative accelerations and other internal transport processes. Rotationally induced transport processes for instance are believed to reduce the effects of atomic diffusion. This will be investigated in a forthcoming companion paper.
Solar energetic particles and galactic cosmic rays over millions of years as inferred from data on cosmogenic26Al in lunar samples
Authors: Poluianov S. , Kovaltsov G. A. , Usoskin I. G. 
Published: July 2018

Aims.Lunar soil and rocks are not protected by a magnetic field or an atmosphere and are continuously irradiated by energetic particles that can produce cosmogenic radioisotopes directly inside rocks at different depths depending on the particle’s energy. This allows the mean fluxes of solar and galactic cosmic rays to be assessed on the very long timescales of millions of years.Methods.Here we show that lunar rocks can serve as a very good particle integral spectrometer in the energy range 20–80 MeV. We have developed a new method based on precise modeling, that is applied to measurements of26Al (half-life ≈0.7 megayears) in lunar samples from theApollomission, and present the first direct reconstruction (i.e., without any a priori assumptions) of the mean energy spectrum of solar and galactic energetic particles over a million of years.Results.We show that the reconstructed spectrum of solar energetic particles is totally consistent with that over the last decades, despite the very different levels of solar modulation of galactic cosmic rays (ϕ= 496 ± 40 MV over a million years versus (ϕ= 660 ± 20 MV for the modern epoch). We also estimated the occurrence probability of extreme solar events and argue that no events with theF(>30 MeV) fluence exceeding 5×1010and 1011cm−2are expected on timescales of a thousand and million years, respectively.Conclusions.We conclude that the mean flux of solar energetic particles hardly depends on the level of solar activity, in contrast to the solar modulation of galactic cosmic rays. This puts new observational constraints on solar physics and becomes important for assessing radiation hazards for the planned space missions.
Risk and Relevance of Insulin Pump Therapy in the Aetiology of Ketoacidosis in People with Type 1 Diabetes
Authors: Giessmann Lena Christina , Kann Peter Herbert 
Published: July 2018

Abstract
Objective The aim of this systematic data analysis was to determine the prevalence of diabetic ketoacidosis (DKA) as well as hypoglycemic and hyperglycemic disorders during insulin pump therapy (CSII) in patients with type 1 diabetes. The main focus was to investigate whether CSII patients have more DKA than the general type 1 diabetes population.
Subjects and Methods This retrospective study with patients who were treated in our treatment center from 2003 to 2016 includes data from 229 patients (52.4% male, 47.6% female, 37.2±16.3 years; DKA: 93, hypoglycemia: 66, hyperglycemia: 70).
Results Intensified insulin therapy was the most common treatment regimen in the study cohort (73.4%), followed by CSII (24%). However, 32.3% of the patients with DKA were on CSII. This number of DKA cases among the insulin pump users in our study cohort was higher than the prevalence reported in a previously published study by Reichel et al. (2013; p<0.05) and in a customer database (p<0.005). Most common causes of DKA in our study cohort were patient errors (43.3%) or insulin resistance induced by an underlying infection (29.8%). Device malfunction caused 13.5% of all DKA cases with an overwhelming majority on insulin pump treatment (93%). Overall, patient errors caused more DKA cases than device malfunctions.
Conclusions Our findings suggest that despite development of more sophisticated insulin pump devices, DKA is still more frequent with CSII than with other kinds of insulin treatment.
Epitope Mapping of Inhibitory Monoclonal Antibodies to Human von Willebrand Factor by Using Recombinant cDNA Libraries
Authors: Piétu Geneviéve , Ribba Anne-Sophie , Chérel Ghislaine , Siguret Virginie , Obert Bernadette , Rouault Christine , Ginsburg David , Meyer Dominique 
Published: July 2018

SummaryTwo recombinant expression libraries containing small (300-600 base pairs) cDNA fragments of von Willebrand Factor (vWF) were screened in order to map the epitope of monoclonal antibodies (MAbs) to vWF. Among eleven MAbs tested, seven were effectively mapped. The epitopes of MAbs 418 and 522, which inhibit the binding of vWF to Factor VIII (FVIII), were localized between Leu 2 and Arg 53 and between Glu 35 and He 81 of the vWF subunit respectively, within the N-terminal trypsin fragment called SpIII-T4 [amino acids (aa) 1-272] which contains a binding domain for FVIII. The epitope of MAb 710, which inhibits the binding of vWF to glycoprotein lb (GPIb), was identified between Ser 593 and Ser 678 on the tryptic 52/48 kDa fragment (aa 449-728) which contains binding domains for GPIb, collagen, heparin, sulfatides and subendothclium extracellular matrices. The epitope of MAb 723, which does not interfere with any known function of vWF, was localized between Ser 523 and Gly 588. The epitopes of MAb 505 and MAb 400, which inhibit the binding of v WF to collagen, were identified between Leu 927 and Arg 1114 within the SPI fragment (aa 911-1365) corresponding to the central part of the vWF subunit. The epitope of MAb 9, which inhibits the binding of vWF to GPIIb/IIIa, was identified in the C-terminal part of the vWF subunit between Gin 1704 and Asp 1746, the latter being the third aa of the RGD sequence common to adhesive proteins and serving as a recognition site for integrin receptors.
Pharmacokinetic Properties of Recombinant Factor VIII Compared with a Monoclonally Purified Concentrate (Hemofil® M)
Authors: Morfini M , Longo G , Messori A , Lee M , White G , Mannucci P ,   The Recombinate Study Group
Published: July 2018

SummaryA recombinant FVIII preparation, Recombinate™, was compared with a high-purity plasma-derived concentrate, Hemofil® M, in 47 hemophilia A patients in a cross-over evaluation of pharmacokinetic properties. The recombinant material showed a significantly lower clearance, volume of distribution, and higher in vivo recovery, but a similar half-life to the plasma-based product.In a comparison with reported data from other standard concentrates, the recombinant preparation exhibited potentially better pharmacokinetic properties in that its clearance was slower and its half-life was longer.We conclude that the recombinant DNA method of preparation does not adversely affect the biological and pharmacological characteristics of the factor VIII molecule.