bluuebunny/crossref_metadata_2025_split huggingface dataset:
Automated label‐free detection of injured neuron with deep learning by two‐photon microscopy
Authors: Wang Shu , Lin Bingbing , Lin Guimin , Lin Ruolan , Huang Feng , Liu Weilin , Wang Xingfu , Liu Xueyong , Zhang Yu , Wang Feng , Lin Yuanxiang , Chen Lidian , Chen Jianxin 
Published: October 2019

AbstractStroke is a significant cause of morbidity and long‐term disability globally. Detection of injured neuron is a prerequisite for defining the degree of focal ischemic brain injury, which can be used to guide further therapy. Here, we demonstrate the capability of two‐photon microscopy (TPM) to label‐freely identify injured neurons on unstained thin section and fresh tissue of rat cerebral ischemia‐reperfusion model, revealing definite diagnostic features compared with conventional staining images. Moreover, a deep learning model based on convolutional neural network is developed to automatically detect the location of injured neurons on TPM images. We then apply deep learning‐assisted TPM to evaluate the ischemic regions based on tissue edema, two‐photon excited fluorescence signal intensity, as well as neuronal injury, presenting a novel manner for identifying the infarct core, peri‐infarct area, and remote area. These results propose an automated and label‐free method that could provide supplementary information to augment the diagnostic accuracy, as well as hold the potential to be used as an intravital diagnostic tool for evaluating the effectiveness of drug interventions and predicting potential therapeutics.
Optimal stimulation frequency for vibrational optical coherence elastography
Authors: Zhang Duo , Wang Jinjiang , Li Chunhui , Huang Zhihong 
Published: October 2019

AbstractVibrational optical coherence elastography (OCE) is a promising tool for extracting the mechanical property of soft tissue. Purpose of this study is focusing on settling the optimal frequency range for vibrational OCE with evenly distributed stress filed. A finite element model of 2% agar phantom was built by ANSYS with a vibration stimulation frequency range from 200 to 3000 Hz. Practical experiments were carried out for cross‐validation with the same frequencies and sample. Lateral and horizontal stress filed distributions under different frequencies were mathematically evaluated by coefficient of variance and degree of linearity. Results from simulation and practical experiment cross‐validated each other and 1000 Hz was set as the maximum ideal frequency for vibrational OCE, while the minimum frequency is set by theoretical calculation with a result of 250 Hz. An ex vivo biological sample was utilised to testify performance of vibrational OCE with excitation frequencies in and out of concluded optimal range, which showed that stiffness was better mapped out in optimal frequency range.
Mineral and Electrolyte Disorders With SGLT2i Therapy
Authors: Cianciolo Giuseppe , De Pascalis Antonio , Capelli Irene , Gasperoni Lorenzo , Di Lullo Luca , Bellasi Antonio , La Manna Gaetano 
Published: October 2019

ABSTRACTThe newly developed sodium‐glucose cotransporter 2 inhibitors (SGLT2is) effectively modulate glucose metabolism in diabetes. Although clinical data suggest that SGLT2is (empagliflozin, dapagliflozin, ertugliflozin, canagliflozin, ipragliflozin) are safe and protect against renal and cardiovascular events, very little attention has been dedicated to the effects of these compounds on different electrolytes. As with other antidiabetic compounds, some effects on water and electrolytes balance have been documented. Although the natriuretic effect and osmotic diuresis are expected with SGLT2is, these compounds may also modulate urinary potassium, magnesium, phosphate, and calcium excretion. Notably, they have had no effect on plasma sodium levels and promoted only small increases in serum potassium and magnesium concentrations in clinical trials. Moreover, SGLT2is may induce an increase in serum phosphate, FGF‐23, and PTH; reduce 1,25‐dihydroxyvitamin D; and generate normal serum calcium. Some published and preliminary reports, as well as unconfirmed reports have suggested an association with bone fractures. Some homeostasis perturbations are transient, whereas others may persist, suggesting that the administration of SGLT2is may affect electrolyte balances in exposed subjects. Although current evidence supports their safety, additional efforts are needed to elucidate the long‐term impact of these compounds on chronic kidney disease, mineral metabolism, and bone health. Indeed, the limited follow‐up studies and the heterogeneity of the case‐mix of different randomized controlled trials preclude a definitive answer on the impact of these compounds on long‐term outcomes such as the risk of bone fracture. Here we review the current understanding of the mechanisms involved in electrolyte handling and the available data on the clinical implications of electrolytes and mineral metabolism perturbations induced by SGLT2i administration. © 2019 The Authors. JBMR Plus published by Wiley Periodicals, Inc. on behalf of American Society for Bone and Mineral Research.
Homogenization of a combined hourly air temperature dataset over Romania
Authors: Dumitrescu Alexandru , Cheval Sorin , Guijarro José A. 
Published: October 2019

AbstractDaily and sub‐daily homogenization of climate variables have been intensively investigated in the last decades, but to the best of our knowledge, this is the first study on homogenization of hourly temperature in Romania. This paper describes the creation of a homogenized hourly air temperature data set at a country scale by combining data from four independent meteorological networks. The air temperature measurements for the period 2009 and 2017 were obtained from the following networks: Romanian National Meteorological Administration (ANM), National Network for Monitoring Air Quality (RNMCA), Regional Basic Synoptic Network (RBSN), and Meteorological Terminal Aviation Routine Weather Report network (METAR). The climatological limits, persistence, temporal variation (step test), and spatial consistency were the quality control tests used to isolate the errors due to malfunctioning of the temperature sensors, data coding or transmission. The Climatol homogenization method was successfully applied for identifying and correcting any suspicious values. The missing data were filled by considering the similarities between each station and the reference series. Comparing the output with the original data, it is apparent that the removal of the break points, correction and homogenization resulted in a new data set with statistical properties very similar to the raw data, but more reliable for climate research due to the increased homogeneity. Eventually, the procedure can be implemented in operational use for collecting more data from other networks.
microRNAs in sudden hearing loss
Authors: Nunez Desmond A. , Wijesinghe Printha , Nabi Shahin , Yeh David , Garnis Cathie 
Published: October 2019

ObjectivesTo compare the circulating microRNA (miRNA) expression profiles between sudden sensory neural hearing loss (SSNHL) patients and age‐matched normal hearing controls.Study DesignProspective cohort multi‐center study.MethodsPatients presenting within 28 days of onset of SSNHL were prospectively recruited along with contemporaneous age‐matched controls. Pooled sera of four patient (n = 09, mean age = 53.0 years; 07, 55.0; 10, 52.9; 10, 51.6) and two control (09, 51.2 and 03, 50.0) groups were assessed using a TaqMan Low Density Array. The patients’ sera were also divided into two pools, untreated (04, 57.7) and treated (32, 52.6) for additional analysis. miRNA expression level was derived from cycle threshold (Ct) values normalized to a global mean. Inter‐group mean Ct differences with fold changes ≥2.0 and ≤0.5 atP < .05 were considered significant. Bioinformatic databases were used to identify putative target mRNAs or validated genes and their functional annotations.ResultsThirty‐six SSNHL patients (mean age 53.0 ± standard deviation (SD) 15.2 years) and 12 controls (50.9 ± 11.9) were studied. Eight miRNAs hsa‐miR‐590‐5p/ ‐186‐5p/ ‐195‐5p/ ‐140‐3p/ ‐128‐3p/ ‐132‐3p/ ‐375‐3p, and ‐30a‐3p were identified as significantly differentially expressed in SSNHL patients. Most of these miRNAs were abundantly identified in the nervous system and the putative target messenger RNAs (mRNAs) were enriched in signaling pathways such as phosphatidyl inositol 3 kinase/protein Kinase B (PI3K/Akt), Ras and mitogen‐activated protein kinase (MAPK).ConclusionThese findings suggest the possible cellular signaling pathways that underlie the disruption of auditory signal transmission in SSNHL.Level of Evidence2Laryngoscope, 130:E416–E422, 2020
Readability of patient‐reported outcome measures for chronic rhinosinusitis and skull base diseases
Authors: Lee Saangyoung E. , Farzal Zainab , Kimple Adam J. , Senior Brent A. , Thorp Brian D. , Zanation Adam M. , Ebert Charles S. 
Published: October 2019

ObjectiveOutcome measures in healthcare that presume a higher level of patient health and overall literacy may inadequately estimate the disease experiences of less‐educated patients and further disadvantage them. Patient‐Reported Outcome Measures (PROMs) are widely used communication tools for clinical practice and are often used to evaluate and guide management for chronic rhinosinusitis (CRS) and skull base diseases. However, their readability and subsequent incomprehensibility for patients have not been assessed. The aim of this study is to evaluate the readability of commonly used PROMs for these conditions and whether they meet recommended readability levels.MethodsThree readability measures, Gunning Fog, Simple Measure of Gobbledygook (SMOG), and FORCAST were used in the evaluation of commonly used PROMs for CRS and skull base disease. PROMs with sixth‐grade readability level or lower were considered to meet health literacy experts' recommendations.ResultsA total of 11 PROMs were reviewed (8 CRS, 3 skull base). Gunning Fog consistently estimated the easiest readability, whereas FORCAST the most difficult. One hundred percent of CRS and 67% of skull base PROMs were above National Institutes of Health and health literacy experts' recommended reading levels. PROMs developed more recently had easier readability.ConclusionPROMs are important clinical tools in otolaryngology that help guide management of disease for improved patient‐centered care. Like many other fields of medicine, those used in otolaryngology are beyond recommended reading levels. Development of PROMs in the future should meet recommended readability levels to fully assess the disease experience of our patients.Level of Evidence4 Laryngoscope, 130:2305–2310, 2020
q‐pseudoconvex and q‐holomorphically convex domains
Authors: Ioniţă George‐Ionuţ , Preda Ovidiu 
Published: October 2019

AbstractIn this article we prove a global result in the spirit of Basener's theorem regarding the relation between q‐pseudoconvexity and q‐holomorphic convexity: we prove that any open subset with smooth boundary, strictly q‐pseudoconvex, is ‐holomorphically convex; moreover, assuming that Ω verifies an additional assumption, we prove that it is q‐holomorphically convex. We also prove that any open subset of is n‐holomorphically convex.
Chitin deacetylases Cod4 and Cod7 are involved in polar growth of Aspergillus fumigatus
Authors: Xie Mingming , Zhao Xiaobing , Lü Yang , Jin Cheng 
Published: October 2019

AbstractChitin is one of the key components of fungal cell wall, and chitin deacetylases (CDAs) have been found in fungi; however, their functions remain unknown. Aspergillus fumigatus is known to cause fatal invasive aspergillosis (IA) among immunocompromised patients with a high mortality rate. Although the A. fumigatus cell wall has long been taken as a unique target for drug development, its dynamic remodeling is complicated and not well understood. Seven putative CDAs are annotated in the A. fumigatus genome. In this study, we analyzed the function of the putative CDAs, Cod4 and Cod7, in A. fumigatus. Biochemical analysis of recombinant proteins showed that Cod4 preferentially deacetylated (GlcNAc)4 and was less active on chitooligosaccharides with DP > 5, whereas Cod7 was unable to catalyze deacetylation. Simulation of three‐dimensional structure revealed that both Cod4 and Cod7 shared a similar folding pattern with HyPgdA from Helicobacter pylori and, similar to HyPgdA, a substitution of Thr8 by Ala8 in Cod7 abolished its CDA activity. Deletion of the cod4, cod7, or both in A. fumigatus led to polarity abnormality and increased conidiation. Furthermore, the expression level of the genes related to polarity was upregulated in the mutants. Our results demonstrated that Cod4 and Cod7 were involved in polarity, though Cod4 was inactive.
Network modelling, citizen science and targeted interventions to predict, monitor and reverse bee decline
Authors: Lander Tonya 
Published: October 2019

Societal Impact StatementThe global decline in pollinating insect populations has rightly received widespread news coverage as it imperils ecosystem function and human food security. Reversing and addressing this decline is an urgent global priority. However, in many locations we do not know what species are present, how large or small species populations are, or what types of specific resources the populations require. By adopting novel network analyses approaches and by working with monitoring programs, such as Oxford Plan Bee, we may be able to dramatically improve our ability to predict species extinctions and facilitate targeted conservation action to maintain abundant, diverse and stable pollinator communities.
Summary
Pollination is fundamentally important to ecosystem function and human food security. Recent reports of dramatic insect declines, and pollinator decline in particular, have increased public awareness and political motivation to act to protect pollinators. This article maps commonly proposed management interventions onto known drivers of bee decline, and identifies forage and nest site provision as a tractable management intervention that can simultaneously address multiple drivers of decline. However, it is recognized that there are gaps in the knowledge of exactly how much and which types of forage resources are necessary to support wild pollinator populations. A novel network analysis approach based on quantified floral resources and pollination services is proposed, which would illuminate the types and quantities of floral resources and pollinators necessary to maintain a diverse and abundant plant–pollinator community. The approach would also facilitate the prediction of species extinctions in plant–pollinator communities and help target conservation interventions. Finally, Oxford Plan Bee is introduced as a new, citizen‐science‐based project to monitor solitary bee populations, and provide empirical data to validate predictions from the proposed network approach. The over‐arching aim of the described network analysis approach and the Oxford Plan Bee project is to facilitate effective, evidence‐based conservation action to protect pollinators and the plants they pollinate into the future.
Gut microbiota metabolic potential correlates with body size between mulberry‐feeding lepidopteran pest species
Authors: Chen Bosheng , Xie Sen , Zhang Xiancui , Zhang Nan , Feng Huihui , Sun Chao , Lu Xingmeng , Shao Yongqi 
Published: October 2019

AbstractBACKGROUNDMany insect pests rely on microbial symbionts to obtain nutrients or for defence, thereby allowing them to exploit novel food sources and degrade environmental xenobiotics, including pesticides. Although Lepidoptera is one of the most diverse insect taxa and includes important agricultural pests, lepidopteran microbiotas, particularly functional traits, have not been studied widely. Here, we provide a comprehensive characterization of the gut microbiota across multiple mulberry‐feeding lepidopteran species, resolving both community structure and metabolic potential.RESULTSOur results indicate abundant bacteria inside the gut of larval Lepidoptera. However, even though they were fed the same diet, the structures of the bacterial communities differed in four major mulberry pest species, suggesting host‐specific effects on microbial associations. Community‐level metabolic reconstructions further showed that although taxonomic composition varied greatly, carbohydrate and amino acid metabolism and membrane transporter were key functional capabilities of the gut bacteria in all samples, which may play basic roles in the larval gut. In addition, principal coordinate analysis (PCoA) of gut bacterial‐predicted gene ontologies revealed specialized features of the microbiota associated with these mulberry pests, which were divided into two distinct clusters (macrolepidopterans and microlepidopterans). This pattern became even more prominent when further Lepidoptera species were involved.CONCLUSIONSA suite of gut microbiota metabolic functions significantly correlated with larval size; the metabolism of terpenoids and polyketides, xenobiotics biodegradation and metabolism were specifically enriched in large species, while small larvae had enhanced nucleotide metabolism. Our report paves the way for uncovering the correlation between host phenotype and microbial symbiosis in this notorious insect pest group. © 2019 Society of Chemical Industry
A bacterium against the tiger: preliminary evidence of fertility reduction after release of Aedes albopictus males with manipulated Wolbachia infection in an Italian urban area
Authors: Caputo Beniamino , Moretti Riccardo , Manica Mattia , Serini Paola , Lampazzi Elena , Bonanni Marco , Fabbri Giulia , Pichler Verena , della Torre Alessandra , Calvitti Maurizio 
Published: October 2019

AbstractBACKGROUNDNovel tools are needed to reduce the nuisance and risk of exotic arbovirus transmission associated with the colonization of temperate regions by Aedes albopictus. The incompatible insect technique (IIT) is a population suppression approach based on cytoplasmic incompatibility between males with manipulated endosymbionts and wild females. Here, we present the results of the first field experiment in Europe to assess the capacity of an Ae. albopictus line (ARwP) deprived of its natural endosymbiont Wolbachia and transinfected with a Wolbachia strain from the mosquito Culex pipiens, to sterilize wild females.RESULTSWe released ∼ 4500 ARwP males weekly for 6 weeks in a green area within urban Rome (Italy) and carried out egg (N = 13 442), female (N = 128) and male (N = 352) collections. Egg (N = 13 783) and female (N = 48) collections were also carried out at two untreated control sites. The percentage of viable eggs during release was, on average, significantly lower in treated sites than in control sites, with the greatest difference (16%) seen after the fourth release. The ARwP to wild male ratio in the release spots between day 3 after the first ARwP male release and day 7 after the last release was, on average, 7:10. Released males survived up to 2 weeks. Approximately 30% of females collected in the release spots showed 100% sterility and 20% showed strongly reduced fertility compared with control sites.CONCLUSIONSResults support the potential of IIT as a tool contributing to Ae. albopictus control in the urban context, and stress the need for larger field trials to evaluate the cost‐efficacy of the approach in suppressing wild populations. © 2019 Society of Chemical Industry
Endoscopic Vacuum Therapy in the Management of Postoperative Leakage After Esophagectomy
Authors: Jeon Jae Hyun , Jang Hyo Joon , Han Ji Eun , Park Young Soo , Seong Yong Won , Cho Sukki , Jheon Sanghoon , Kim Kwhanmien 
Published: October 2019

AbstractBackgroundPostoperative leakage after esophagectomy is associated with significant life‐threatening complications. Recently, endoscopic vacuum therapy (EVT) was introduced and has been successfully used as a new treatment option. The purpose of this study was to evaluate the safety and efficacy of EVT for the management of postoperative leakage after esophagectomy.MethodsA total of 22 patients were treated with either intraluminal or intracavitary EVT for the management of postoperative leakage from May 2012 to April 2018. The location of leakage was intrathoracic in 17 patients and cervical in five patients. The outcomes of EVT were analyzed retrospectively.ResultsComplete postoperative leakage closure was achieved in 19 of 22 patients. The median duration of EVT application was 14 days (range 2–103), and a median number of three EVT systems (range 1–14) were used. In 19 patients who were successfully managed with EVT, oral feeding was possible a median of 15 days after the first day of treatment. There were no cases of mortality related to postoperative leakage.ConclusionsEVT is a well‐tolerated and effective therapeutic option for the treatment of various types of postoperative leakage after esophagectomy.
Viscosity dynamics and the production of extracellular polymeric substances and soluble microbial products during anaerobic digestion of pulp and paper mill wastewater sludges
Authors: Ekstrand Eva-Maria , Svensson Bo H. , Šafarič Luka , Björn Annika 
Published: October 2019

Abstract
The production processes of the pulp and paper industry often run in campaigns, leading to large variations in the composition of wastewaters and waste sludges. During anaerobic digestion (AD) of these wastes, the viscosity or the production of extracellular polymeric substances (EPS) and soluble microbial products (SMP) may be affected, with the risk of foam formation, inefficient digester mixing or poor sludge dewaterability. The aim of this study was to investigate how viscosity and production of EPS and SMP during long-term AD of pulp and paper mill sludge is affected by changes in organic loading rate (OLR) and hydraulic retention time (HRT). Two mesophilic lab-scale continuous stirred tank reactors (CSTRs) were operated for 800 days (R1 and R2), initially digesting only fibre sludge, then co-digesting fibre sludge and activated sludge. The HRT was lowered, followed by an increase in the OLR. Reactor fluids were sampled once a month for rheological characterization and analysis of EPS and SMP. The production of the protein fraction of SMP was positively correlated to the OLR, implicating reduced effluent qualities at high OLR. EPS formation correlated with the magnesium content, and during sulphate deficiency, the production of EPS and SMP increased. At high levels of EPS and SMP, there was an increase in viscosity of the anaerobic sludges, and dewatering efficiency was reduced. In addition, increased viscosity and/or the production of EPS and SMP were important factors in sludge bulking and foam formation in the CSTRs. Sludge bulking was avoided by more frequent stirring.