bluuebunny/crossref_metadata_2025_split huggingface dataset:
The structural basis of nanobody unfolding reversibility and thermoresistance
Authors: Kunz Patrick , Zinner Katinka , Mücke Norbert , Bartoschik Tanja , Muyldermans Serge , Hoheisel Jörg D. 
Published: May 2018

AbstractNanobodies represent the variable binding domain of camelid heavy-chain antibodies and are employed in a rapidly growing range of applications in biotechnology and biomedicine. Their success is based on unique properties including their reported ability to reversibly refold after heat-induced denaturation. This view, however, is contrasted by studies which involve irreversibly aggregating nanobodies, asking for a quantitative analysis that clearly defines nanobody thermoresistance and reveals the determinants of unfolding reversibility and aggregation propensity. By characterizing nearly 70 nanobodies, we show that irreversible aggregation does occur upon heat denaturation for the large majority of binders, potentially affecting application-relevant parameters like stability and immunogenicity. However, by deriving aggregation propensities from apparent melting temperatures, we show that an optional disulfide bond suppresses nanobody aggregation. This effect is further enhanced by increasing the length of a complementarity determining loop which, although expected to destabilize, contributes to nanobody stability. The effect of such variations depends on environmental conditions, however. Nanobodies with two disulfide bonds, for example, are prone to lose their functionality in the cytosol. Our study suggests strategies to engineer nanobodies that exhibit optimal performance parameters and gives insights into general mechanisms which evolved to prevent protein aggregation.
Investigation on the structures and magnetic properties of carbon or nitrogen doped cobalt ferrite nanoparticles
Authors: Cao Derang , Pan Lining , Li Jianan , Cheng Xiaohong , Zhao Zhong , Xu Jie , Li Qiang , Wang Xia , Li Shandong , Wang Jianbo , Liu Qingfang 
Published: May 2018

AbstractCarbon or nitrogen doped cobalt ferrite nanoparticles were synthesized in the air by a facile calcination process. X-ray diffraction, mapping, X-ray photoelectron spectroscopy, and mössbauer spectra results indicate that the nonmetal elements as the interstitial one are doped into cobalt ferrite nanoparticles. The morphologies of doped cobalt ferrite nanoparticles change from near-spherical to irregular cubelike shapes gradually with the increased carbon or nitrogen concentration, and their particles sizes also increase more than 200 nm. Furthermore, the saturation magnetization of carbon doped cobalt ferrite is improved. Although the saturation magnetization of N-doped cobalt ferrite is not enhanced obviously due to the involved hematite, they also do not drop drastically. The results reveal an approach to synthesize large scale ferrite nanoparticles, and improve the magnetic properties of ferrite nanoparticles, and also provide the potential candidates to synthesis co-doped functional magnetic materials.
Automatic Cone Photoreceptor Localisation in Healthy and Stargardt Afflicted Retinas Using Deep Learning
Authors: Davidson Benjamin , Kalitzeos Angelos , Carroll Joseph , Dubra Alfredo , Ourselin Sebastien , Michaelides Michel , Bergeles Christos 
Published: May 2018

AbstractWe present a robust deep learning framework for the automatic localisation of cone photoreceptor cells in Adaptive Optics Scanning Light Ophthalmoscope (AOSLO) split-detection images. Monitoring cone photoreceptors with AOSLO imaging grants an excellent view into retinal structure and health, provides new perspectives into well known pathologies, and allows clinicians to monitor the effectiveness of experimental treatments. The MultiDimensional Recurrent Neural Network (MDRNN) approach developed in this paper is the first method capable of reliably and automatically identifying cones in both healthy retinas and retinas afflicted with Stargardt disease. Therefore, it represents a leap forward in the computational image processing of AOSLO images, and can provide clinical support in on-going longitudinal studies of disease progression and therapy. We validate our method using images from healthy subjects and subjects with the inherited retinal pathology Stargardt disease, which significantly alters image quality and cone density. We conduct a thorough comparison of our method with current state-of-the-art methods, and demonstrate that the proposed approach is both more accurate and appreciably faster in localizing cones. As further validation to the method’s robustness, we demonstrate it can be successfully applied to images of retinas with pathologies not present in the training data: achromatopsia, and retinitis pigmentosa.
Vanishing river ice cover in the lower part of the Danube basin – signs of a changing climate
Authors: Ionita M. , Badaluta C. -A. , Scholz P. , Chelcea S. 
Published: May 2018

AbstractMany of the world’s largest rivers in the extra tropics are covered with ice during the cold season, and in the Northern Hemisphere approximately 60% of the rivers experience significant seasonal effects of river ice. Here we present an observational data set of the ice cover regime for the lower part of the Danube River which spans over the period 1837–2016, and its the longest one on record over this area. The results in this study emphasize the strong impact of climate change on the occurrence of ice regime especially in the second part of the 20th century. The number of ice cover days has decreased considerably (~28days/century) mainly due to an increase in the winter mean temperature. In a long-term context, based on documentary evidences, we show that the ice cover occurrence rate was relatively small throughout the Medieval Warm Period (MWP), while the highest occurrence rates were found during the Maunder Minimum and Dalton Minimum periods. We conclude that the river ice regime can be used as a proxy for the winter temperature over the analyzed region and as an indicator of climate-change related impacts.
A multi-phenotypic imaging screen to identify bacterial effectors by exogenous expression in a HeLa cell line
Authors: Collins Adam , Huett Alan 
Published: May 2018

AbstractWe present a high-content screen (HCS) for the simultaneous analysis of multiple phenotypes in HeLa cells expressing an autophagy reporter (mcherry-LC3) and one of 224 GFP-fused proteins from the Crohn’s Disease (CD)-associated bacterium, Adherent Invasive E. coli (AIEC) strain LF82. Using automated confocal microscopy and image analysis (CellProfiler), we localised GFP fusions within cells, and monitored their effects upon autophagy (an important innate cellular defence mechanism), cellular and nuclear morphology, and the actin cytoskeleton. This data will provide an atlas for the localisation of 224 AIEC proteins within human cells, as well as a dataset to analyse their effects upon many aspects of host cell morphology. We also describe an open-source, automated, image-analysis workflow to identify bacterial effectors and their roles via the perturbations induced in reporter cell lines when candidate effectors are exogenously expressed.
A collection of public transport network data sets for 25 cities
Authors: Kujala Rainer , Weckström Christoffer , Darst Richard K. , Mladenović Miloš N , Saramäki Jari 
Published: May 2018

AbstractVarious public transport (PT) agencies publish their route and timetable information with the General Transit Feed Specification (GTFS) as the standard open format. Timetable data are commonly used for PT passenger routing. They can also be used for studying the structure and organization of PT networks, as well as the accessibility and the level of service these networks provide. However, using raw GTFS data is challenging as researchers need to understand the details of the GTFS data format, make sure that the data contain all relevant modes of public transport, and have no errors. To lower the barrier for using GTFS data in research, we publish a curated collection of 25 cities' public transport networks in multiple easy-to-use formats including network edge lists, temporal network event lists, SQLite databases, GeoJSON files, and the GTFS data format. This collection promotes the study of how PT is organized across the globe, and also provides a testbed for developing tools for PT network analysis and PT routing algorithms.
Investigating the environmental and socio‐economic impacts of grid‐tied photovoltaic and on‐shore wind systems in Bangladesh
Authors: Nurunnabi Md , Roy Naruttam Kumar , Mahmud Md Apel 
Published: May 2018

Current power generation scenarios all over the world are not climate friendly as the generation systems are mainly dependent on fossil fuels that produce greenhouse gas (GHG). As renewable energies (especially solar and wind energies) have the sustainable characteristics, by replacing the conventional energy system with them, it is possible to significantly contribute to reducing the dependency on fossil fuels as well as GHG emissions. Though biomass is the primary renewable source in Bangladesh, this study is exploring new options in photovoltaic (PV) and on‐shore wind in the 0.5–2 MW capacity range. This research presents environmental and socio‐economic impacts of grid‐connected hybrid (PV/wind) power systems by investigating the potentials of the solar and wind energy with considering various sensitivity variables for two different locations, namely Magnama and Dinajpur, in Bangladesh. The main goal of this research is to generate the lowest possible adverse socio‐economic and environmental impacts ensuring a certain degree of monetary benefits. Three sizes of plants have been chosen for quantifying the variations in socio‐economic and environmental impacts. It is found that the proposed model of the hybrid power system can maximise the benefits by reducing the adverse effects of conventional power plants.
VLTI-GRAVITY measurements of cool evolved stars
Authors: Wittkowski M. , Rau G. , Chiavassa A. , Höfner S. , Scholz M. , Wood P. R. , de Wit W. J. , Eisenhauer F. , Haubois X. , Paumard T. 
Published: May 2018

Context. Dynamic model atmospheres of Mira stars predict variabilities in the photospheric radius and in atmospheric molecular layers which are not yet strongly constrained by observations.
Aims. Here we measure the variability of the oxygen-rich Mira star R Peg in near-continuum and molecular bands.
Methods. We used near-infrared K-band spectro-interferometry with a spectral resolution of about 4000 obtained at four epochs between post-maximum and minimum visual phases employing the newly available GRAVITY beam combiner at the Very Large Telescope Interferometer (VLTI).
Results. Our observations show a continuum radius that is anti-correlated with the visual lightcurve. Uniform disc (UD) angular diameters at a near-continuum wavelength of 2.25 μm are steadily increasing with values of 8.7 ± 0.1 mas, 9.4 ± 0.1 mas, 9.8 ± 0.1 mas, and 9.9 ± 0.1 mas at visual phases of 0.15, 0.36, 0,45, 0.53, respectively. UD diameters at a bandpass around 2.05 μm, dominated by water vapour, follow the near-continuum variability at larger UD diameters between 10.7 mas and 11.7 mas. UD diameters at the CO 2–0 bandhead, instead, are correlated with the visual lightcurve and anti-correlated with the near-continuum UD diameters, with values between 12.3 mas and 11.7 mas.
Conclusions. The observed anti-correlation between continuum radius and visual lightcurve is consistent with an earlier study of the oxygen-rich Mira S Lac, and with recent 1D CODEX dynamic model atmosphere predictions. The amplitude of the variation is comparable to the earlier observations of S Lac, and smaller than predicted by CODEX models. The wavelength-dependent visibility variations at our epochs can be reproduced by a set of CODEX models at model phases between 0.3 and 0.6. The anti-correlation of water vapour and CO contributions at our epochs suggests that these molecules undergo different processes in the extended atmosphere along the stellar cycle. The newly available GRAVITY instrument is suited to conducting longer time series observations, which are needed to provide strong constraints on the model-predicted intra- and inter-cycle variability.
Limonoids from Aphanamixis polystachya Leaves and Their Interaction with Hsp90
Authors: Camero César Muñoz , Vassallo Antonio , De Leo Marinella , Temraz Abeer , De Tommasi Nunziatina , Braca Alessandra 
Published: May 2018

AbstractA phytochemical study of n-hexane, CHCl3, and CHCl3-MeOH extracts of Aphanamixis polystachya leaves led to the isolation of 10 compounds. Five of them turned out to be new natural compounds, including two mexicanolide-type (1, 2) and three polyoxyphragmalin-type (3–5) limonoids, together with two known andirobin-type limonoids (6, 7) and three phenolic derivatives. The structures of the new compounds were established on the basis of spectroscopic methods to be 8-hydro-14,15-en-cabralin (1), 3-deacetyl-8-hydro-cabralin-14,15-en-3-one (2), 20,22-dihydroxy-21,23-dimethoxytetrahydrofuran khayanolide A (3), 1-deacetyl-3-dehydroxy-3-oxokhaysenelide E (4), and meliaphanamixin A (5). All compounds were isolated for the first time from this species. The ability of the isolated limonoids to interact with the molecular chaperone Hsp90 was tested. Compounds 6 and 7 were the most active.
Massive Retropharyngeal Hematoma with Airway Obstruction after Minor Injury
Authors: Sobti Shivender , Grewal Sarvpreet , John Paul , Grover Ashwani 
Published: May 2018

AbstractRetropharyngeal hematoma can cause life-threatening airway compromise. We present a case of massive retropharyngeal hematoma following minor injury. The patient required immediate tracheostomy followed by evacuation of hematoma and cervical stabilization.
Role of Magnetic Resonance Spectroscopy for Prognosis of Patients with Traumatic Brain Injury
Authors: Kumar Raghuvendra , Ghosh Subhasis , Dhibar Tapan , Kumar Abhishek 
Published: May 2018

Abstract
Background Traumatic brain injury (TBI) is one of the leading causes of death worldwide. Long-term clinical outcome following TBI can be difficult to predict. Evaluation of the degree of severity of injury and prediction of outcome are important for the management of these patients.
Objective To evaluate whether degree of severity of injury and outcome in moderate to severe TBI is possible by proton magnetic resonance spectroscopy (1H-MRS).
Materials and Methods Patients with moderate (Glasgow coma scale [GCS] 9–13) and severe head injury (GCS: 5–8), within 1 week of trauma with their normal computed tomographic (CT) scan findings, their magnetic resonance imaging (MRI) finding, and neurologic status were investigated with single-voxel proton MRS (1H-MRS). The study included 51 patients and 24 controls.
Result The MRS study revealed lower ratio of N-acetylaspartate (NAA)/choline (Cho) and NAA/creatine (Cr) and higher ratio of Cho/Cr and lactate level compared with the control group. The ratio of NAA/Cr, NAA/Cho, and Cho/Cr were statistically significant with initial GCS (p = < 0.00001, r = 0.7595; p = < 0.00001, r = 0.7506; and p < 0.00001, r = −0.5923, respectively), and these ratios were also statistically significant with Glasgow outcome scale (GOS) (p < 0.00001, r = 0.8498, p < 0.00001, r =0.9323, p < 0.00001, r = −0.9082, respectively). The ratio of NAA/Cr, NAA/Cho, and Cho/Cr were also statistically significant with severity of injury (p < 0.0001).
Conclusion MRS can quantify damage after TBI and may be a method of assessing severity and outcome in TBI.
Cerebral Infarction in a Child after Moderate Brain Trauma: A Case Report
Authors: Gohil Dhaval , Devi Bhagavatula , Bhat Dhananjaya , Shukla Dhaval 
Published: May 2018

AbstractIntracranial hematomas and contusions are common entity after traumatic brain injury in children and young population. However, cerebral infarction after traumatic head injury is a rare entity with frequency ranging from 1.9 to 10.4%. We herein report a case of cerebral infarction in right anterior cerebral artery (ACA) and parts of middle cerebral artery (MCA) territory after moderate head injury in a 14-year-old boy, in whom computed tomographic (CT) scan of the brain was performed 3.5 hours after injury, which showed specks of pneumocephalus in sellar suprasellar region, and 12 hours after injury showed right ACA territory infarct and evolving infarct in right MCA territory. Bony injury included right orbital roof and right sphenoid wing linear undisplaced fractures. Possible mechanism for development of infarction is right internal carotid artery (ICA) dissection by fractured bone fragments and/or thrombosis. The patient developed hemiplegia on second day after trauma. Intracranial pressure (ICP) monitoring was done, which was suggestive of raised ICP. The patient underwent right fronto-temporo-parieto craniotomy and decompression (tissue sent for histopathologic analysis) and augmentative duraplasty with bone flap replacement on fifth day after trauma. Histopathology was suggestive of cerebral infarct with hemorrhage. The patient was improved at follow-up, 10 months after trauma after rehabilitation, and physiotherapy to modified Rankin scale (mRS) score 3. Hence delayed CT scan plays a vital role in detecting developing posttraumatic infarcts, and rigorous rehabilitation care is necessary for clinical improvement.
Relation between extracellular Chemistry and Patient Outcome for Severe Traumatic Brain Injury within the First 24 hours: A Microdialysis Study
Authors: Igarashi Yutaka , Yokobori Shoji , Onda Hidetaka , Masuno Tomohiko , Yokota Hiroyuki 
Published: May 2018

Abstract
Object Many studies have reported that extracellular chemistry is related to the outcome of patients with traumatic brain injury (TBI). No study has reported that extracellular chemistry predicts outcome in less than 3 days. Moreover, in other studies, both focal brain and diffuse brain injuries have been often discussed. The authors focused on the relationship between extracellular chemistry in a shorter period and the outcome of patients with focal brain injury.
Methods By using intracerebral microdialysis monitoring, extracellular fluid concentrations of glucose, lactate, glycerol, glutamate, lactate/pyruvate (L/P), and lactate/glucose (L/G) were determined in 30 patients with severe TBI for initial 24 hours. The results were analyzed between favorable and unfavorable, and between survival and mortality.
Results The medians of glycerol and L/P in the favorable group were significantly lower than those in the unfavorable group (124 µmol/L vs. 808 µmol/L, p = 0.002; 31 vs. 48, p = 0.021, respectively). All parameters apart from glutamate differed significantly between the survival and mortality groups (glucose, 25 mmol/L vs. 77 mmol/L, p = 0.035; lactate, 38 mmol/L vs. 73 mmol/L, p = 0.018; glycerol, 168 µmol/L vs. 1462 µmol/L, p = 0.002; glutamate, 14 µmol/L vs. 95 µmol/L, p = 0.019; L/P, 32 vs. 124, p < 0.001; L/G, 1.46 vs. 4.52, p = 0.004).
Conclusion Cerebral extracellular glycerol and L/P was the most reliable predictor of outcomes in patients with focal brain injury and can discriminate between favorable and unfavorable outcomes for the first 24 hours, using the threshold of 200 and 40, respectively.
Cranial Vault Fractures in Civilian Head Injury: Clinical and Radiologic Predictors of Seizures and the Fracture Seizure Index: A Prospective Single-Center Observational Cohort Study
Authors: Uche Enoch , Okorie Emeka , Onyia Ephraim , Iloabachie Izuchukwu , Matthew Mesi , Amuta Dubem , Mezue Wilfred 
Published: May 2018

Abstract
Study Objectives To determine the risk factors of posttraumatic seizures (PTS) among patients with civilian head injury and skull vault fractures.
Methods A 5-year prospective cohort study of patients with skull fractures presenting to our center from March 2013 to February 2017.
Results Of 637 patients with traumatic brain injury (TBI), 135 (21.2%) patients sustained calvarial fractures, 91 (72%) were from road traffic accidents (RTAs), and most were young adult males (M:F = 11.6, mean age = 29.1 ± 2.3 years, 95% CL). Linear fractures in 69 patients and depressed fractures in 50 (39.7%) were the common fracture types. Seventy-seven (61.1%) patients had cerebral contusions, 31 (24.6%) had extradural hematoma (EDH), and 21 (16.7%) patients had Glasgow coma scale (GCS) ≤ 8. Twenty-five (18.5%) patients suffered early PTS, and five had late PTS. Among nonfracture patients (n = 361), 31 (8.6%) had seizures. Seizures occurred more in the fracture subgroup (χ2
= 10.1, p < 0.05, df = 1) and earlier (χ2
= 5.9, p = 0.027, df = 1). Fractures and seizures followed a similar trend in occurrence. Among patients with vault fractures, severe head injury, contusions, and intracranial hematoma, the relative risk and odds for early seizures followed a trend predicted by a statistical index—the fracture seizure index (FSI). Late PTS did not show a statistical relationship with early PTS (χ2
= 2.98, df = 1, p > 0.05).
Conclusion GCS score ≤8, depressed fracture and multifocal cerebral contusions are predictors of early PTS. Seizure risk associated with fractures and other lesions can be predicted by the FSI.
Unusual Clinical and Imaging Presentation of Chronic Subdural Hematoma
Authors: Roy Kaushik , Saha Suniti K. , Ghosh Partha 
Published: May 2018

AbstractVarious clinical and radiologic presentations of chronic subdural hematoma (SDH) are reported in the literature. Therefore, sometimes the presentation of a patient with chronic SDH often creates confusion regarding decision making. Here, the authors present three cases of chronic SDH, in which the clinical presentation, radiology, and operative findings were unusual. In the first case, the patient presented with acute extradural hematoma like clinical as well as radiologic presentation but intraoperatively found to have chronic calcified SDH, whereas another case with history of bilateral ventriculoperitoneal (VP) shunting at childhood presented with large head with discharging sinus at the forehead. Radiologic and operative findings were very much unusual. Intraoperatively, the bilateral subdural collection was found to have fungus-like projections with subdural space communicating with the forehead sinus. In another case, a 10-year-old girl with history of VP shunting at age of 6 months presented with left hemiparesis of subacute onset. Computed tomographic (CT) scan revealed biconvex lesion at the right parietal region intraoperatively. The authors found the shell-like lesion with inner and outer membrane calcified, within which the subdural collection was present. In these three cases, they observed the very unusual mode of presentation of chronic SDH, and in the literature such mode of presentation and operative findings of such type are very rare.