bluuebunny/crossref_metadata_2025_split huggingface dataset:
Recombinant Escherichia coli Strain with Enhanced Production of Vibrio cholerae Neuraminidase
Authors: Monakhova E. V. , Demidova G. V. , Pisanov R. V. , Duvanova O. V. , Mishan’kin B. N. 
Published: July 2019

Objectiveof this work was cloning of the Vibrio cholerae nanH gene as part of a plasmid vector, providing expression of foreign genes under the control of T5 promoter, and construction of a E. coli strain – producer of V. cholerae recombinant neuraminidase.Materials and methods. V. cholerae о1 strain served as a DNA donor, pQE30 – as a vector plasmid. The gene was PCR-amplifi ed, the cloning was carried out by means of conventional methods, performance of recombinants and localization of the required protein was determined based on the results of electrophoresis of cell lysates. Neuraminidase activity was identifi ed by fl uorescence in ultraviolet light after incubation with specifi c substrate (4-methylumbelliferyl-N-acetylneuraminic acid).Results and discussion. Recombinant plasmid pNanH, containing the cloned gene nanH V. cholerae, has been constructed. The gene is inserted into BamHI-PstI sites of the polylinker of pQE30. Expression of the cloned gene in the producer strain E. coli JM103pNanH occurs under the control of T5 promoter after isopropyl-1-thio-β-D-galactopyranoside (IPTG) induction. The strain shows neuraminidase activity. The recombinant NanH protein is accumulated in the producer’s cells in two forms. The fi rst form, with molecular mass (MM) of 89.5 kDa, is an unprocessed protein with the hexahistidine block (6His-tag) at its N-terminus, it is located in inclusion bodies. Its percentage is 5.6–6.6 % of the total cell proteins. The second one, with MM of 83 kDa, is found in the periplasmic space and corresponds to the mature NanH, its percentage being 3.4–3.8 %. The total percentage of both forms is 9–10 % of total cell proteins, which allows to consider the strain E. coli JM103pNanH to be a super-producer of the required protein. The strain may be used for purifi cation of NanH preparations for construction of specifi c diagnostic, therapeutic and pharmaceutical preparations as well as for investigation of the protein as a virulence/persistence factor of the pathogen.
Tick-Borne Viral Encephalitis Incidence in the Constituent Entities of the Russian Federation. Communication 2. Verification of Conformity of the Forecast Data and Seasonal Monitoring of Actual Morbidity Rates
Authors: Nikitin A. Ya. , Andaev E. I. , Noskov A. K. , Pakskina N. D. , Yatsmenko E. V. , Verigina E. V. , Balakhonov S. V. 
Published: July 2019

Abstract. Despite the decreasing TBE incidence trend in Russia, the disease is considered an ongoing challenge for the state’s public health and economics. Objective of our study was to describe the algorithm of short-term incidence forecast of TBE, to evaluate the conformity of these data to factual incidence and the results of annual strategic seasonal monitoring which takes place across all the entities of Russia. In the paper, we described the procedure for providing short-term extrapolation of TBE incidence forecast onto the Russian territories, depending on the absence or presence of incidence change trends.Materials and methods. Utilized were the State statistics, “The data on infectious and parasitic diseases” (Form No 2), as well as the information on strategic monitoring for a period of 2007–2018. In order to determine the multi-year trend of epidemic process development, regression analysis was applied. If the trend was identified, predictions were made on its basis, if not – through calculating the long-term annual average. In all the cases, 95 % confidence interval for incidence trend deviation was considered. Comparative analysis of actual morbidity rates and predicted ones and the data on strategic monitoring was conducted by Student’s criterion.The commutations were performed using Excel software tools.Results and discussion. The study has demonstrated that the expected rates of TBE incidence are not statistically different from the actual incidence or the data from strategic monitoring. The underestimation of the epidemiological risk is found only in 4 out of 49 entities (8,2 %), and it is of note that in 3 of them it was less than 16 %. The data from operational monitoring are downward biased by reference to actual incidence, which is probably due to inclusion of TBE cases confirmed and/or manifested upon termination of incubation period after expiration of terms of weekly observations. The unified and simple approach that we proposed to TBE-incidence forecasting within the territory of Russia provides for correct information on expected epidemiological risk assessment and timely planning of required preventative measures.
Explicit Determination of Reserves for Variable-Bottomhole-Pressure Conditions in Gas Rate-Transient Analysis
Authors: Wang Yang , Ayala Luis F. 
Published: July 2019

Summary
Current rate-transient-analysis tools for gas wells producing under boundary-dominated-flow (BDF) conditions largely rely on the deployment of the Arps empirical decline models (Arps 1945), or liquid-based analytical models rewritten in terms of pseudofunctions. Recently, Stumpf and Ayala (2016) demonstrated that, contrary to common practice, decline exponents (b) used in Arps’ hyperbolic equations when applied to gas-well analysis can be rigorously estimated before any field-production data are collected. This determination is solely dependent on gas pressure/volume/temperature (PVT) properties and prevailing constant-bottomhole-pressure (BHP) specification for volumetric, single-phase gas-flow conditions. In the study, we extend that work to a more-realistic variable-BHP condition, which is the most common production-specification condition, in terms of the ratio of changing BHP to average reservoir pressure. The decline exponent (b) is thus rederived, and it is shown that under such conditions, variable BHP hyperbolic decline coefficients become solely dependent on fluid PVT properties and take their largest possible magnitude compared with constant-BHP production. Step-by-step analysis procedures are presented that enable explicit and straightforward estimation of original gas in place (OGIP) and other reservoir properties by universal-type-curve and straight-line analysis. Finally, several cases using simulated and field data are discussed in detail to validate the capabilities of the proposed approach.
Online reported women's experiences of symptomatic pelvic organ prolapse after vaginal birth
Authors: Mirskaya Maria , Lindgren Eva-Carin , Carlsson Ing-Marie 
Published: July 2019

Abstract
Background: Pelvic organ prolapse (POP) is a major public health problem with a relative high lifetime risk of surgery. The main risk factor for developing POP is vaginal birth. Many women become symptomatic later in life and most of the existing research on symptomatic pelvic organ prolapse (sPOP) predominantly concentrates on the effects of the condition on postmenopausal women. However, bothersome symptoms of POP can be reported as early as in women’s 20s and may occur shortly after vaginal birth. Limited studies provide an insight into daily life of fertile women with sPOP. Thus, we aimed to explore fertile women's experiences of symptomatic pelvic organ (sPOP) after vaginal birth. Methods: An inductive, text-driven approach was taken by selecting thread posts from an internet forum written by 33 Swedish fertile women who had experienced sPOP after vaginal birth.
Results: The overarching theme “being irreparably damaged” was identified as representing an experience of being disabled by sPOP after vaginal birth. The fertile women experienced that their lives were ruined because of physical and psychological limitations caused by this unexpected, unfamiliar and unexplained condition. Living with sPOP impinged on sexual health, restricted daily and sports activities and affected the women´s ability to fulfill everyday parental duties. This in turn compromised women’s psychological health. In addition, the negligence of healthcare professionals who tended to trivialize and normalize the symptoms led to the belief that there were no sustainable treatments and that women would have to live with bothersome symptoms of POP for rest of their lives.
Conclusions: This study found that sPOP had a significant negative impact on fertile women’s lives. The women indicated that they had not had the opportunity to voice their concerns and had not been taken seriously by healthcare professionals. It is of the utmost importance to acknowledge this problem and develop guidelines for prevention and management of sPOP to improve the quality of life for women.
A novel echocardiographic parameter for assessment of right ventricular function in acute pulmonary embolism: an experimental study
Authors: Zeng Decai , Wu Ji , Chen Hui , Tan Ying , Che Xueyu , Zhang Ting 
Published: July 2019

Abstract
Background

Right ventricular (RV) dysfunction played a decisive role in clinical management and associated with poor prognosis in acute pulmonary embolism (PE). It still remains challenging to estimate RV function accurately for the reason of complex structure and geometry. The present study aimed to determine the value of right ventricular outﬂow tract systolic excursion (RVOT-SE) in evaluating RV function in an animal model with acute PE.

Methods

Thirty-three healthy New Zealand rabbits were randomly assigned to massive thrombus group, sub-massive thrombus group and control group, 11 rabbits per group. The acute PE model was established by intravenous infusion of autologous blood clots. After 1h of thrombus injection, transthoracic echocardiography was performed to assess RV function in all rabbits.

Results

The acute PE model was successfully made in 18 rabbits (massive thrombus group, n = 8; sub-massive thrombus group n = 10). Right/left ventricular end-diastolic diameter (RV/LV) ratio and RV myocardial performance (Tei) index were significantly increased, while RV fractional area change (RVFAC), tricuspid annular plane systolic excursion (TAPSE) and RVOT_SE were reduced in massive thrombus group. The value of RVOT-SE and RVFAC in sub-massive thrombus group decreased significantly compared with control group (P < 0.05). But there was no significant difference in RV/LV, TAPSE and Tei index (P > 0.05). ROC analysis showed that RVOT-SE had high sensitivity (94.4%) and specificity (72.7%) in identifying RV dysfunction in acute PE. The area under the ROC curve (AUC) for combined TAPSE and RVOT-SE was greater than that of TAPSE or RVOT-SE alone (AUC= 0.962, P < 0.01).

Conclusion

RV function in acute PE is significantly decreased, which is closely related to the size of embolus. RVOT_SE is a simple and highly distinctive parameter in identifying RV dysfunction and tends to be superior to conventional parameters in acute PE. The combination of RVOT-SE and TAPSE can further improve the diagnostic accuracy of acute PE.
Ruxolitinib shows activity against Hodgkin lymphoma but not primary mediastinal large B-cell lymphoma
Authors: Kim Seok Jin , Yoon Dok Hyun , Kang Hye Jin , Hong Jung Yong , Lee Ho Sup , Oh Sung Yong , Shin Ho-Jin , Kong Jee Hyun , Yi Jun Ho , Sakamoto Kana , Ko Young Hyeh , Huh Jooryung , Lee Seung-Sook , Takeuchi Kengo , Shin Dong-Yeop , Suh Cheolwon , Kim Won Seog 
Published: July 2019

Abstract
Background

The upregulated expression of the JAK/STAT pathway promotes tumor growth in Hodgkin lymphoma (HL) and primary mediastinal large B-cell lymphoma (PMBCL). Based on the hypothesis that JAK2 is a therapeutic target, we performed a prospective pilot study using ruxolitinib.

Methods

Relapsed or refractory patients with HL or PMBCL were eligible for this study, and JAK2 amplification was assessed by fluorescence in situ hybridization. Ruxolitinib was administered orally at a dose of 20 mg twice daily for a 28-day cycle. Treatment was continued for up to 16 cycles or until progressive disease or intolerability. The primary objective was to assess the overall disease control rate comprising complete response (CR), partial response (PR), or stable disease (SD).

Results

We analyzed 13 HL patients and six PMBCL patients. All responders (one CR, five PR, and one SD) had HL whereas all cases of PMBCL progressed after first or second cycle. The disease control rate for HL was 54% (7/13) with median response duration of 5.6 months. JAK2 amplification was present in six of nine patients tested (four HL, two PMBCL), and three of these HL patients showed PR (n = 2) or SD (n = 1). None of the three HL patients shown to not have JAK2 amplification responded to ruxolitinib. Most treatment-related adverse events were grade 1 or 2 and manageable.

Conclusions

Ruxolitinib has single-agent activity against HL but does not act against PMBCL with or without JAK2 amplification.
Implementasi Metode Inquiry Discovery untuk Pembentukan Kreativitas Mahasiswa dalam Seni Penyajian Makanan
Authors: Komariah Kokom 
Published: July 2019

Kreativitas dapat dikembangkan melalui berbagai cara, sementara ini implementasi inquiry dan descovery pada praktek seni penyajian makanan belum pernah dikaji. Melalui tulisan ini ingin diketahui apakah penggunaan metode inquiry. Discovery berdampak pada pembentukan kreativitas seni penyajian makanan. Penelitian ini dilakukan melalui penelitian tindakan kelas, metode penelitian dimodifikasi dari Kemmis, setiap siklus dibagi 3 tahap yaitu perencanaan, observasi dan tindakan serta refleksi. Subjek penelitian adalah 39 orang mahasiswa. Pengumpulan data melalui observasi, angket dan wawancara, Analisis data dilakukan secara deskiptif kuantitatif. Hasil penelitian menemukan metode inquiry discovery menunjukkan kreativitas proses sangat baik (3,44) dan kreativitas produk baik (3,97), dan kreativitas individu secara rerata sangat baik (3,33). Dengan demikian proses inquiry dapat membentuk peserta didik untuk berkreasi pada aktivitas seni penyajian makanan, hal ini dapat dibuktikan dengan produk yang dihasilkan lebih bervariasi tidak terpancang pada contoh yang diberikan oleh dosen, dan gambar atau model yang di dapatkan melalui internet.
Tata Rias Fantasi Lionfish Lepu Dalam Pergelaran Drama Musikal Sabda Raja Mutiara
Authors: Novita Elok , Nadilah Shabrina 
Published: July 2019

Penelitian ini adaptasi dari penulisan proyek akhir mahasiswa D3 Tata Rias& Kecantikan yang menggelar acara drama musikal sabda raja mutiara. Adapun tujuan pembuatannya untuk memperlihatkan proses hasil rias fantasi mulai dari merancangtata rias fantasi, merancang kostum dan kelengkapanya sampai aplikasi rias fantasi dan menata kostum dengan kelengkapannya pada tokoh Lionfish Lepu dan mempergelarkan drama musikal Sabda Raja Mutiara yang terdapat tokoh Lionsfish Lepu. Metode yang digunakan untuk endapatkan hasil rias fantasi tokoh Lionfish Lepu menggunakan R&D dengan model pengembangan 4D, yaitu: 1) define; 2) design; 3) develop; 4) disseminate. Waktu penelitian dilakukan di lab tata rias dan kecantikan lt 2 program studi Tata Rias& Kecantikan , jurusan PTBB, FT-UNY. Hasil yang diperoleh dari proyek akhir ini, yaitu: 1) rancangan tata rias fantasi dan rancangan kostum serta kelengkapannya; 2) terwujudnya aplikasi tata rias fantasi berupa ; 3) terselenggara penampilan tokoh Lionfish Lepu dalam sebuah pergelaran di auditorium UNY 26 Januari 2017 pukul 13.00 wib yang dihadiri 850 penonton.
Pengembangan Video Pembelajaran Dodot Pengantin Puteri Gaya Solo Basahan
Authors: Juniastuti Eni , Yuswati Yuswati , Tritanti Asi 
Published: July 2019

Tujuan dari penelitian ini adalah: 1) untuk mengembangkan naskah program video pembelajaran Dodot Pengantin Putri Gaya Solo Basahan, 2) untuk menguji kelayakan video pembelajaran Dodot Pengantin Putri Gaya Solo Basahan. Penelitian ini merupakan penelitian pengembangan (research and development). Subyek penelitian adalah mahasiswa semester 3 dan semester 5 prodi D3 Tata Rias dan kecantikan FT UNY tahun angkatan 2008 yang sudah mengambil Mata Kuliah Pengantin Indonesia II dan angkatan 2009 yang belum mengambil Mata Kuliah Pengantin Indonesia II. Data penelitian dianalisis dengan teknik deskriptif kuantitatif secara sederhana, yakni dengan membandingkan nilai rerata. Hasil penelitian menunjukkan bahwa: 1) Pengembangan Video Pembelajara Dodot pengantin gaya solo basahan terdiri dari beberapa tahapan antara lain: a)Terdapat perencanaan dalam merumuskan tujuan, disesuaikan dengan kebutuhan mahasiswa agar dapat tercapai kompetensi dibidang Tata Rias Pengantin.b) Pembuatan produk Video Pembelajara Dodot pengantin gaya Solo Basahan dengan prosedur yang direncanakan.c) Uji coba Video untuk memvalidasi produk, dengan validasi ahli materi,dan ahli media yang berkompeten di bidang Tata Rias Pengantin Terutama penganti Gaya Solo Basahan . d) Merevisi hasil produk dan memperbaiki sebelum dilakukan uji coba dilapangan.e) Uji coba produk dilapangan atau pada pengguna produk tersebut.f) membuat laporan hasil sesuai dengan data yang diperoleh dilapangan baik dari ahli materi, ahli media dan pengguna Video Dodot pengantin gaya Solo Basahan 2) penelitian Video Pembelajaran Dodot Pengantin Putri Gaya Solo Basahan Puteri ini dalam kategori sangat layak sebagai sumber belajar menurut ahli media dan ahli materi, dan baik berdasarkan hasil uji coba di lapangan.
Perfil de pacientes e a ocorrência de complicações após cirurgia cardiovascular em hospital quaternário
Authors: Pereira Karen Thalita , Silva Bárbara Santucci da , Soares Naury de Jesus Danzi , Hueb Alexandre Ciappina 
Published: July 2019

Introdução:As complicações no pós-operatório de cirurgia cardiovascular podem aumentar o tempo de internação, a taxa de mortalidade e os custos hospitalares. Objetivo: Conhecer as características dos pacientes submetidos à cirurgia cardiovascular e identificar as complicações ocorridas no pós-operatório, durante o tempo de internação, em hospital quaternário. Métodos: Estudo unicêntrico, retrospectivo, transversal e quantitativo. Foram analisados 90 prontuários de pacientes submetidos a cirurgia cardiovascular, de junho de 2014 a julho de 2017. Resultados: Maior prevalência de pacientes do sexo masculino (67,8%), média de idade de 63,8 ± 3,4 anos e hipertensos (90%). O procedimento mais realizado foi a revascularização do miocárdio e o óbito entre esses pacientes foi de 3,33%. A circulação extracorpórea foi utilizada em 78% das cirurgias. Maioria dos pacientes ficou internada por menos de 24 h até a realização do procedimento. No pós-operatório, o tempo de ventilação mecânica foi inferior a 24 h e a droga mais utilizada foi o cloridrato de dobutamina. A complicação arrítmica foi a mais comum, com frequência de 22%. Conclusão:Não foi possível observar associação entre a pre-sença de fatores de risco e a ocorrência de com-plicações intra-hospitalares nos pacientes em pós-operatório de cirurgia cardiovascular.
Penyelesaian Sengketa Perkebunan Antara Perusahaan Perkebunan Sawit PT. Asiatic Persada dengan Suku Anak Dalam Batin IX di Desa Bungku Kecamatan Bajubang Kabupaten Batanghari
Authors: Fitria Fitria 
Published: July 2019

Penelitian ini bertujuan untuk mengetahui dan menganalisis Mekanisme penyelesaian sengketa perkebunan antara perusahaan perkebunan sawit PT. Asiatic Persada dengan Suku Anak Anak Dalam Batin 9 Desa Bungku dan mengetahui dan meganalisis Perananan Pemerintah Daerah terhadap penyelesaian sengketa perkebunan antara perusahaan perkebunan sawit PT. Asiatic Persada dengan Suku Anak Anak Dalam Batin 9 Desa Bungku. Pendekatan yang digunakan bersifat yuridis empiris, secara yuridis melihat peraturan perundang-undangan yang mengatur tentang Penyelesaian sengketa Perkebunan Antara Perusahaan Perkebunan Sawit PT. Asiatic Persada dengan Suku Anak Dalam Batin 9 Di Desa Bungku Kecamatan Bajubang Kabupaten Batanghari. Penelitian ini diharapkan bahwa Sengketa yang terjadi diantara Suku Anak Dalam dengan PT.Asiatic dan latar belakang kasus yang terjadi adalah sengketa pertanahan dan perkampungan masyarakat Suku Anak Dalam (SAD) Kelompok 113 tiga dusun (Tanah Menang, Pinang Tinggi, Padang Salak) yang berada di areal HGU sebuah perusahaan kelapa sawit yang belum dibebaskan. Akan tetapi, hingga saat ini, kesepakatan tersebut belum dilaksanakan oleh pihak perusahaan.
Model Pengelolaan Pertambangan Batubara Berbasis Penguatan Kelembagaan Masyarakat Adat di Kabupaten Bungo
Authors: Syarif Afif 
Published: July 2019

Pertambangan batubara sebagai kekayaan alam yang tak terbarukan, pengelolaannya harus dilakukan seoptimal mungkin, efesien, transparan, berkelanjutan dan bewawasan lingkungan, serta berkeadilan agar memperoleh manfaat sebesar-besarnya kemakmuran rakyat secara berkelanjutan. Untuk itu kepastian hukum pengelolaan pertambangan batubara dapat memberikan kontribusi dalam mewujudkan keadilan bidang pertambangan batubara di Kabupaten Bungo. Desain yang akan dilakukan dalam penelitian ini adalah menggunakan metode penelitian hukum normatif dengan pendekatan deskriptif analitis yang bertujuan untuk : (1) mencari konsep dan/atau model pengelolaan pertambangan batubara berbasis penguatan kelembagaan masyarakat adat dan hak-asasi manusia atas lingkungan hidup; (2) mengkaji konsep keadilan terhadap pengelolaan pertambangan batubara sebagai sumber daya alam; serta (3) melihat kewenangan pemerintah daerah terhadap pengelolaan pertambangan batubara di kabupaten Bungo. Untuk itu perlu dikaji model pengelolaan pertambangan batubara yang dapat memberikan konstribusi kongkrit dalam rangka terciptanya pembangunan berkelanjutan yang berkeadilan terhadap sumber daya alam batubara di Kabupaten Bungo.
Kajian Tentang Sport Development Index Kota Jambi Tahun 2017
Authors: Indrayana Boy 
Published: July 2019

Penelitian ini mengkaji tentang Sport Development Index (SDI) Kota Jambi : Kecamatan Telanai Pura , Kecamatan Jambi Pasar dan Kecamatan Kota Baru dengan empat dimensi , yaitu dimensi Partisipasi Masyarakat Berolahraga , Dimensi Ruang Terbuka ,dimensi Sumber Daya Manusia, dimensi Kebugaran. Tujuan penelitian ini adalah : 1) untuk mendapatkan seberapa besar tingkat partisipasi masyarakat berolahraga di Kota Jambi khususnya Kecamatan Telanai Pura, kecamatan Jambi Pasar dan Kecamatan Kota Baru, 2) untuk mendapatkan informasi tentang berapa luas ruang terbuka yang tersedia untuk berolahraga di Kota Jambi Khususnya Kecamatan Telanai Pura, Kecamatan Jambi Pasar dan Kecamatan Kota Baru, 3) Untuk mendapatkan informasi tentang tingkat kebugaran masyarakat di Kota Jambi khususnya Kecamatan Telanai Pura, Keamatan Jambi Pasar dan Kecamatan Kota Baru. Penelitian ini dilakukan selama 6 bulan meliputi persiapan sampai pelaporan. Penelitian menggunakan metode yang digunakan dalam penelitian ini adalah metode deskriptif Survey dengan angket dan tes pengukuran. Metode penelitian ini adalah deskriptif survey dengan angket dan tes pengukuran terhadap 270 Orang. Hasil penelitian diperoleh dimensi setiap kecamatan yaitu, Kecamatan Telanaipura, Partisipasi Masyarakat Berolahraga sebesar 0.72, Ruang Terbuka Sebesar 0.254, Kebugaran sebesar 0.39 dan SDM sebesar 0.20. Kecamatan Jambi Pasar Partisipasi Masyarakat Berolahraga sebesar 0.82, Ruang Terbuka sebesar 0.408 , kebugaran sebesar 0.37 dan SDM sebesar 0.24. Sedangkan Kecamatan Kota Baru Partisipasi Masyarakat Berolahraga Sebesar 0.75, Ruang Terbuka sebesar 1.18, Kebugaran sebesar , 0.39 dan SDM sebesar 0.82.
Analisis Yuridis Perjanjian Yang Berklausula Arbitrase Dalam Penyelesaian Sengketa Perdagangan
Authors: Alissa Evalina , Bafadhal Faizah 
Published: July 2019

Penelitian ini bertujuan untuk menganalisis kewenangan absolut dari lembaga Arbitrase dan penyelesaian sengketa alternatif dalam menyelesaikan sengketa perdagangan (bisnis) yang perjanjiannya berklausula arbitrase, serta menganalisis dasar hukum yang berlaku dalam menyelesaikan sengketa perdagangan atau bisnis yang berklausula Arbitrase. Dalam penyelesaian sengketa perdagangan atau bisnis yang di dalam perjanjian pokoknya mencantumkan perjanjian penyelesaiaan sengketanya berklausula arbitrse, maka itu merupakan kewenangan absolut dari lembaga arbitrase ,hal ini diatur dalam Undang-Undang Nomor 30 tahun 1999, Undang-Undang tentang Arbitrase dan Penyelesaian Sengketa Alternatif, dalam Pasal 3 dan Pasal 11.