bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genomic Vulnerability to Climate Change of an Australian Migratory Freshwater Fish, the Golden Perch (Macquaria ambigua)
Authors: Booth Emily J. , Brauer Chris J. , Sandoval‐Castillo Jonathan , Harrisson Katherine , Rourke Meaghan L. , Attard Catherine R.M. , Gilligan Dean M. , Tonkin Zeb , Thiem Jason D. , Unmack Peter J. , Zampatti Brenton , Beheregaray Luciano B. 
Published: November 2024

ABSTRACTGenomic vulnerability is a measure of how much evolutionary change is required for a population to maintain optimal genotype‐environment associations under projected climates. Aquatic species, and in particular migratory ectotherms, are largely underrepresented in studies of genomic vulnerability. Such species might be well equipped for tracking suitable habitat and spreading diversity that could promote adaptation to future climates. We characterised range‐wide genomic diversity and genomic vulnerability in the migratory and fisheries‐important golden perch (Macquaria ambigua) from Australia's expansive Murray–Darling Basin (MDB). The MDB has a steep hydroclimatic gradient and is one of the world's most variable regions in terms of climate and streamflow. Golden perch are threatened by fragmentation and obstruction of waterways, alteration of flow regimes, and a progressively hotter and drying climate. We gathered a genomic dataset of 1049 individuals from 186 MDB localities. Despite high range‐wide gene flow, golden perch in the warmer, northern catchments had higher predicted vulnerability than those in the cooler, southern catchments. A new cross‐validation approach showed that these predictions were insensitive to the exclusion of individual catchments. The results raise concern for populations at warm range edges, which may already be close to their thermal limits. However, a population with functional variants beneficial for climate adaptation found in the most arid and hydrologically variable catchment was predicted to be less vulnerable. Native fish management plans, such as captive breeding and stocking, should consider spatial variation in genomic vulnerability to improve conservation outcomes under climate change, even for dispersive species with high connectivity.
What intrinsic factors affect the central corneal thickness?
Authors: Da Silva Filipe , Linhares João M. M. , Lira Madalena 
Published: November 2024

AbstractThe cornea is one of the tissues responsible for covering and protecting the inner structures of the eye. Central corneal thickness (CCT) is defined as the distance between the anterior epithelial surface and the posterior surface of the endothelial layer. This parameter plays a very important role regarding intraocular pressure (IOP) measurement, evaluation of corneal uniformity, selection of a suitable technique for corneal refractive surgery and the planning of surgical procedures to overcome corneal disease. This comprehensive review elucidates the multifaceted factors influencing the central corneal thickness. Recognising the impact of these factors not only enhances our understanding of corneal dynamics but also contributes significantly to the refinement of diagnostic and therapeutic strategies in ophthalmology.
Documenting customary land boundaries using unmanned aerial vehicle imagery and artificial intelligence
Authors: Abeho Dianah Rose , Shoko Moreblessings , Odera Patroba Achola 
Published: November 2024

AbstractThe use of computer vision and deep learning in boundary documentation for land registration stems from the ongoing demand for appropriate mapping approaches of unregistered land rights to eradicate the global challenge of tenure insecurity. Previous research has yielded promising results towards automated extraction of photo‐visible cadastral boundaries from high‐resolution imagery. Nonetheless, the extraction of invisible cadastral boundaries is still a challenge. This study investigates the place of sensor/s on‐board unmanned aerial vehicles and deep learning algorithms in detecting cadastral boundaries. It develops a participatory boundary marking procedure using low‐cost markers to bring monument to previously invisible and ill‐defined cadastral boundaries. After that, the researchers trained and tested the accuracy of a convolutional neural network, namely single shot multi‐box detector (SSD) based on Residual Neural Network (ResNet) and Visual Geometry Group (VGG) backbone networks to automatically detect cadastral boundary markers from unmanned aerial vehicle imagery. SSD based on ResNet34 performed best with 0.88 precision, 0.92 recall and 0.91 F measure or (F1) score. VGG19‐based SSD yielded a precision of 0.47, recall of 0.53 and F1 score of 0.50. The horizontal accuracy of the cadastral map generated varied from 0.089 to 0.496 m per parcel, with a standard deviation of 0.120 m. Results show that this approach is practical for cadastral mapping in rural areas.
Periodontal diseases in Africa
Authors: Olujitan Mojisola , Ayanbadejo Patricia O. , Umeizudike Kehinde , Oyapero Afolabi , Okunseri Christopher , Butali Azeez 
Published: November 2024

AbstractPeriodontal diseases, a group of complex conditions marked by an excessive immune response and periodontal tissue destruction, are a global health concern. Since 1990, the incidence of these diseases has doubled, with Western sub‐Saharan Africa experiencing the highest burden. Accurate diagnosis and case identification are crucial for understanding the etiology, features of disease, research, treatment and prevention. Modern perspectives on periodontal disease classification are based on commonality among those affected. However, current literature is often plagued by methodological inconsistencies and focused on disease mechanisms in European populations. Health inequalities in low‐ and middle‐income countries (LMICs) are exacerbated by these challenges, with sub‐Saharan Africa, and Nigeria specifically, facing unique difficulties such as clinical personnel shortages and limited research infrastructure. This review explored disparities in periodontal disease research, care and outcomes in African populations. We highlighted these disparities and identified the factors contributing to inequities in periodontal health outcomes. We further demonstrated the critical need for inclusive and equitable healthcare and research practices tailored to the unique challenges faced by diverse populations and regions with limited resources. Addressing these disparities is essential for ensuring that advancements in healthcare are accessible to all, thereby improving global oral health and general health.
The immediate and lasting effects of imagery rescripting and their associations with imagery tendency in young adults with childhood maltreatment history: An ERP study
Authors: Liu Mingfan , Niu Juan , Zhou Li , Zeng Yuandong , Ouyang Huan 
Published: November 2024

AbstractThe effectiveness of imagery rescripting (IR) in reducing psychological symptoms associated with aversive memories has been confirmed across various disorders. To better understand the neural mechanisms underlying IR, we assessed the immediate and lasting effects and their associations with imagery tendency by using unpleasant pictures depicting child maltreatment within a population with childhood maltreatment (CM) history. Participants (n = 68) were instructed to engage in two experimental phases while electroencephalogram was recorded. In the rescripting phase, participants viewed neutral or unpleasant pictures and then either imagined the same pictures or rescripted unpleasant ones to assess immediate effect. In the re‐exposure phase, participants passively viewed all pictures without instruction to assess lasting effect. Participants rated their subjective valence and imagery vividness in the rescripting phase or intensity of negative feelings in the re‐exposure phase. IR led to an attenuation of the late positive potential (LPP) amplitude in the late time window (2000–6000 ms at parietal‐occipital electrodes) and a decrease in self‐reported unpleasantness during the rescripting phase. After 5‐min interval, unpleasant pictures with rescripted history elicited smaller LPP (400–1500 ms at centro‐parietal electrodes) and negative feelings than those with imagery history in the re‐exposure phase. The higher habitual use of imagery was associated with a greater reduction in late LPP during the rescripting phase and full‐time range LPP during the re‐exposure phase. The current findings suggest that IR has an immediate effect and a lasting effect on subjective and neural response in the CM population. Individuals with higher imagery tendency are likely to profit more from IR.
Overexpression of TAF4B Promoted the Proliferation of Undifferentiated Spermatogonia in Cattleyak In Vitro
Authors: Tian Yuan , Zhang Peng , Jing Kemin , Li Yuqian , Yue Binglin , Wu Zhijuan , Dong Wenjing , Zhong Jincheng , Cai Xin 
Published: November 2024

ABSTRACTAs the hybrid between cattle and yak, cattleyak is a typical male sterile mammal, and the underlying mechanism for its spermatogenic arrest is still unclear. In this study, the coding region of cattleyak TAF4B gene was cloned by RT‐PCR and analysed by bioinformatics. To investigate the effects of TAF4B on cellular proliferation and differentiation, an expression vector was generated and introduced into undifferentiated spermatogonia (UDSPG) of cattleyak. The results showed that the protein encoded by TAF4B did not contain the signal peptide sequence. The expression level of TAF4B in UDSPG of cattleyak was lower than that in yak, while the overexpression of TAF4B in cattleyak promoted the proliferation activity of cattleyak UDSPG. Meanwhile, the expression of proliferation and meiosis‐related genes was increased but the differentiation‐related genes were decreased. Therefore, the aberrant expression of TAF4B in cattleyak UDSPG possibly impaired its proliferation and differentiation equilibrium and decreased its growth potentiality, thereby reducing the quantity of UDSPG and affecting spermatogenesis. This study provided a potential approach for further elucidation of the mechanism of spermatogenesis arrest and provided a new idea for solving the problem of male infertility in cattleyak.
Benchmark dose modeling for epidemiological dose–response assessment using case‐control studies
Authors: De Pretis Francesco , Zhou Yun , Shao Kan 
Published: November 2024

AbstractFollowing a previous article that focused on integrating epidemiological data from prospective cohort studies into toxicological risk assessment, this paper shifts the focus to case‐control studies. Specifically, it utilizes the odds ratio (OR) as the main epidemiological measure, aligning it with the benchmark dose (BMD) methodology as the standard dose–response modeling approach to determine chemical toxicity values for regulatory risk assessment. A standardized BMD analysis framework has been established for toxicological data, including input data requirements, dose–response models, definitions of benchmark response, and consideration of model uncertainty. This framework has been enhanced by recent methods capable of handling both cohort and case‐control studies using summary data that have been adjusted for confounders.The present study aims to investigate and compare the “effective count” based BMD modeling approach, merged with an algorithm used for converting odds ratio to relative risk in cohort studies with partial data information (i.e., the Wang algorithm), with the adjusted OR‐based BMD analysis approach. The goal is to develop an adequate BMD modeling framework that can be generalized for analyzing published case‐control study data. As in the previous study, these methods were applied to a database examining the association between bladder and lung cancer and inorganic arsenic exposure. The results indicate that estimated BMDs and BMDLs are relatively consistent across both methods. However, modeling adjusted OR values as continuous data for BMD estimation aligns better with established practices in toxicological BMD analysis, making it a more generalizable approach.
Support needed by parents when a child's mental well‐being is threatened—A qualitative study of views of experts‐by‐experience
Authors: Tiina Putkuri , Irene Latva‐Korpela , Mikko Häkkinen 
Published: November 2024

AbstractBackgroundIn situations where a child's mental well‐being is threatened, the parents also need support. The available support has been perceived as insufficient by both parents and professionals.AimTo explore the views of experts‐by‐experience of the support needed by parents when a child's mental well‐being is threatened.MethodsA qualitative study with a phenomenological approach was conducted in Finland. The data were collected in six focus group interviews during the autumn of 2022. The participants (n = 26) were adult experts‐by‐experience who had experienced either mental well‐being challenges in their own childhood (before the age of 18 years) or experienced the mental well‐being challenges of a child from the role of a parent. The data were analysed using inductive content analysis.ResultsThe support needed by parents in situations where the mental well‐being of their child is at risk consists of support for parenting, support for sharing and support for surviving.ConclusionParents whose child's mental well‐being is at risk, need support both for their own well‐being and for their ability to support their child's well‐being. Support is needed not only from professionals but also from peers.
Personalized CZA‐ATM dosing against an XDR E. coli in liver transplant patients; the application of the in vitro hollow fiber system
Authors: Sadouki Zahra , Wey Emmanuel Q. , Iype Satheesh , Nasralla David , Potts Jonathan , Spiro Mike , Williams Alan , McHugh Timothy D. , Kloprogge Frank 
Published: November 2024

AbstractBackgroundA patient with an extensively drug‐resistant (XDR) New Delhi metallo‐β‐lactamase (NDM) and oxacillinase (OXA‐48) producing Escherichia coli (E. coli) infection was awaiting orthotopic liver transplant. There is no standardized antibiotic prophylaxis regimen; however, in line with the Infectious Diseases Society of America guidance, an antibiotic prophylactic regimen of ceftazidime‐avibactam 2.5 g TDS with aztreonam 2 g three times a day (TDS) IV was proposed.MethodsThe hollow fiber system (HFS) was applied to inform the individualized pharmacodynamic outcome likelihood prior to prophylaxis.ResultsA 4‐log reduction in CFU/mL in the first 10 h of the regimen exposure was observed; however, the killing dynamics were slow and six 8‐hourly infusions were required to reduce bacterial cells to below the limit of quantification. Thus, the HFS supported the use of the regimen for infection clearance; however, it highlighted the need for several infusions. Standard local practice is to administer prophylaxis antibiotics at induction of orthotopic liver transplantation (OLT); however, the HFS provided data to rationalize earlier dosing. Therefore, the patient was dosed at 24 h prior to their OLT induction and subsequently discharged 8 days after surgery.ConclusionThe HFS provides a dynamic culture solution for informing individualized medicine by testing antibiotic combinations and exposures against the bacterial isolates cultured from the patient's infection.
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The dynamic N1‐methyladenosine RNA methylation provides insights into the tomato fruit ripening
Authors: Ma Lili , Zuo Jinhua , Bai Chunmei , Fu Anzhen , Wang Qing , Zhou Zhongjing , Deng Zhiping , Tan Jinjuan , Bouzayen Mondher , Zheng Yanyan 
Published: November 2024

SUMMARYN1‐methyladenosine (m1A) methylation is an essential mechanism of gene regulation known to impact several biological processes in living organisms. However, little is known about the abundance, distribution, and functional significance of mRNA m1A modification during fruit ripening of tomato the main model species for fleshy fruits. Our study shows that m1A modifications are prevalent in tomato mRNA and are detected in lncRNA and circRNA. The distribution of m1A peaks in mRNA segments indicates that m1A is mainly enriched at the start codon and CDS regions. Assessing changes in global RNA methylation during fruit ripening in wild‐type tomatoes and in the ripening‐impaired Nr mutant affected in the ethylene receptor gene (SlETR3) revealed a decrease in the overall methylation levels from mature green (MG) stage to 6 days postbreaker (Br + 6). Nr mutant fruits show significantly lower methylation levels than Ailsa Craig (AC) fruits. Notably, differences in m1A methylation are well correlated to the expression levels of a number of key ripening‐related genes. The integration of RNA‐seq and MeRIP‐seq data suggests a potential positive impact of m1A modifications on gene expression. In comparison to the AC fruits, the hypomethylation and reduced expression of ethylene‐related genes, ACO3, EBF1, and ERF.D6, in the Nr mutants likely underpin the distinct phenotypic traits observed between the two fruit genotypes at the Br6 stage. Overall, our study brings further arguments supporting the potential significance of m1A methylation modifications in fruit ripening, a developmental process that is instrumental to plant reproduction and to fruit sensory and nutritional qualities.
A plastidial lipoyl synthase LIP1p plays a crucial role in phosphate homeostasis in Arabidopsis
Authors: Ge Shenghong , Xiao Xinlong , Zhang Ke , Yang Changhong , Dong Jinsong , Chen Keying , Lv Qiuyu , Satheesh Viswanathan , Lei Mingguang 
Published: November 2024

SUMMARYPhosphate (Pi) homeostasis is important for plant growth and adaptation to the dynamic environment, which requires the precise regulation of phosphate transporter (PHT) trafficking from the endoplasmic reticulum to the plasma membrane. LIPOYL SYNTHASE 1p (LIP1p) is known as a key enzyme in plastids to catalyze lipoylation of pyruvate dehydrogenase complex for de novo fatty acid synthesis. It is unknown whether this process is involved in regulating Pi homeostasis. Here, we demonstrate a new role of LIP1p in controlling Pi homeostasis by regulating PHT1 trafficking. We recovered a weak mutant allele of LIP1p in Arabidopsis that accumulates much less Pi and has enhanced expression of phosphate starvation‐induced genes. LIP1p mutation alters the lipid profile and compromises vesicle trafficking of PHT1 to the plasma membrane to impair Pi uptake. Beside phosphorus, the homeostasis of a series of mineral nutrients was also perturbed in lip1p mutant. Our findings provide powerful genetic evidence to support the linkage between lipoylation and ion homeostasis in plants.
ABI3 regulates ABI1 function to control cell length in primary root elongation zone
Authors: Datta Saptarshi , Mandal Drishti , Mitra Sicon , Chakraborty Swarnavo , Nag Chaudhuri Ronita 
Published: November 2024

SUMMARYPost‐embryonic primary root growth is effectively an interplay of several hormone signalling pathways. Here, we show that the ABA‐responsive transcription factor ABI3 controls primary root growth through the regulation of JA signalling molecule JAZ1 along with ABA‐responsive factor ABI1. In the absence of ABI3, the primary root elongation zone is shortened with significantly reduced cell length. Expression analyses and ChIP‐based assays indicate that ABI3 negatively regulates JAZ1 expression by occupying its upstream regulatory sequence and enriching repressive histone modification mark H3K27 trimethylation, thereby occluding RNAPII occupancy. Previous studies have shown that JAZ1 interacts with ABI1, the protein phosphatase 2C, that works during ABA signalling. Our results indicate that in the absence of ABI3, when JAZ1 expression levels are high, the ABI1 protein shows increased stability, compared to when JAZ1 is absent, or ABI3 is overexpressed. Consequently, in the abi3‐6 mutant, due to the higher stability of ABI1, reduced phosphorylation of plasma membrane H+‐ATPase (AHA2) occurs. HPTS staining further indicated that abi3‐6 root cell apoplasts show reduced protonation, compared to wild‐type and ABI3 overexpressing seedlings. Such impeded proton extrusion negatively affects cell length in the primary root elongation zone. ABI3 therefore controls cell elongation in the primary root by affecting the ABI1‐dependent protonation of root cell apoplasts. In summary, ABI3 controls the expression of JAZ1 and in turn modulates the function of ABI1 to regulate cell length in the elongation zone during primary root growth.
ACVECC‐Veterinary Committee on Trauma registry report 2020–2021
Authors: Talbot Charles T. , Raffe Marc R. , Boller Manuel , Edwards Melissa L. , Hall Kelly E. 
Published: November 2024

AbstractObjectiveTo report summative data from the American College of Veterinary Emergency and Critical Care (ACVECC)‐Veterinary Committee on Trauma (VetCOT) registry, with further individual evaluation of university and private practices and level I and II Veterinary Trauma Centers (VTCs).DesignMulti‐institutional registry data report, January 1, 2020, to December 31, 2021.SettingVTCs identified and verified by ACVECC‐VetCOT.AnimalsDogs and cats with evidence of trauma.InterventionsData were input to a web‐based data capture system (REDCap) by data entry personnel trained in registry software use and operational definitions of data variables. Patient data on demographics, trauma type, preadmission care, trauma severity assessment at presentation (modified Glasgow Coma Score and Animal Trauma Triage score), key laboratory parameters, interventions, and outcome were collected. Summary descriptive data for each species are reported.Measurements and Main ResultsTwenty‐two VTCs contributed data to the VetCOT registry during a 24‐month period, culminating in a total of 9758 complete trauma case records for dogs and 11734 for cats. Head trauma in dogs and cats was seen at a higher percentage in both university‐only VTCs (encompassing both level I and II) (20.1% and 24.1%, respectively) and level I‐only VTCs (24.3% and 24.1%, respectively), in comparison to private‐only VTCs (encompassing both level I and II) (13.5% and 16.2%, respectively) and individual level II VTCs (14.1% and 18.9%, respectively). Canine and feline surgical procedures were performed at a higher percentage at university VTCs (50% and 40.5%, respectively) compared to private VTCs (39.2% and 28.6%, respectively). Overall survival to discharge for dogs and cats remains high at 93.1% and 83.6%, respectively.ConclusionsThe VetCOT registry has continued to show powerful potential in collating a large, multifaceted, international dataset in trauma for both dogs and cats. As published in previous VetCOT registry reports, survival to discharge has remained static across both university and private practice veterinary hospitals; however, further breakdown has identified university and level I VTCs admitting and managing a higher number of head traumas, as well as university VTCs performing a higher proportion of surgical procedures. Data from this registry will continue to aid in the design of clinical trials, prospective observational studies, and translational research, which will improve the understanding and outcome of trauma patients.