bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reduced inflammatory state promotes reinnervation of endometriotic-like lesions in TNFRp55 deficient mice
Authors: Ghersa F , Delsouc M B , Goyeneche A A , Vallcaneras S S , Meresman G , Telleria C M , Casais M 
Published: May 2019

AbstractEndometriosis is a chronic gynecological disease, characterized by growth of endometrial tissue in ectopic sites due to alteration of peritoneal homeostasis and deregulation of apoptosis. Here we have examined whether TNFRp55 deficiency modulates the pro-inflammatory state and the reinnervation of endometriotic-like lesions in mice. Two-month-old female C57BL/6 mice, eight wild type (WT) and eight TNFRp55−/− (KO) were used in the study. Endometriotic-like lesions were induced experimentally. The right uterine horn was removed from the animal, divided longitudinally, cut in three square pieces and sutured to the intestine mesentery. After 4 weeks, the lesions and the peritoneal fluid were collected. The level of TNFα in the peritoneal fluid was evaluated by enzyme-linked immunosorbent assay (EIA). The expressions of COX2, GRα and GRβ were evaluated in the lesions by western blot and immunohistochemistry. β-III TUBULIN, BDNF and NGF protein concentrations were evaluated in the lesions by western blot. Gene expression of Pgp 9.5, SP and Th was analyzed by RT-PCR, whereas relative concentrations of TRKA, NTRp75, phosphorylated NFκB (pNFκB) and total NFκB in lesions were measured by EIA. Compared with the WT group, the KO mice showed lower TNFα levels in the peritoneal fluid and lower numbers of COX2 immunoreactive cells along with increased expression of GRα, β-III TUBULIN, Pgp 9.5, SP, Th, BDNF, NGF, NTRp75 and pNFκB in the lesions. Future histological studies will be necessary to confirm the sensory/sympathetic imbalance in the endometriotic-like lesions of the KO mice. Our results suggest that a reduced inflammatory state promotes reinnervation of endometriotic-like lesions in TNFRp55−/− mice. Chronic deregulation of TNF receptors can have serious consequences for women with advanced endometriosis.
Resting-State Functional Network Organization Is Stable Across Adolescent Development for Typical and Psychosis Spectrum Youth
Authors: Jalbrzikowski Maria , Liu Fuchen , Foran William , Roeder Kathryn , Devlin Bernie , Luna Beatriz 
Published: May 2019

AbstractBackgroundResting-state functional neuroimaging captures large-scale network organization; whether this organization is intact or disrupted during adolescent development across the psychosis spectrum is unresolved. We investigated the integrity of network organization in psychosis spectrum youth and those with first episode psychosis (FEP) from late childhood through adulthood.MethodsWe analyzed data from Philadelphia Neurodevelopmental Cohort (PNC; typically developing = 450, psychosis spectrum = 273, 8–22 years), a longitudinal cohort of typically developing youth (LUNA; N = 208, 1–3 visits, 10–25 years), and a sample of FEP (N = 39) and matched controls (N = 34). We extracted individual time series and calculated correlations from brain regions and averaged them for 4 age groups: late childhood, early adolescence, late adolescence, adulthood. Using multiple analytic approaches, we assessed network stability across 4 age groups, compared stability between controls and psychosis spectrum youth, and compared group-level network organization of FEP to controls. We explored whether variability in cognition or clinical symptomatology was related to network organization.ResultsNetwork organization was stable across the 4 age groups in the PNC and LUNA typically developing youth and PNC psychosis spectrum youth. Psychosis spectrum and typically developing youth had similar functional network organization during all age ranges. Network organization was intact in PNC youth who met full criteria for psychosis and in FEP. Variability in cognitive functioning or clinical symptomatology was not related to network organization in psychosis spectrum youth or FEP.DiscussionThese findings provide rigorous evidence supporting intact functional network organization in psychosis risk and psychosis from late childhood through adulthood.
Decent Care for Migrant Households: Policy Alternatives to Sri Lanka’s Family Background Report
Authors: Withers Matt 
Published: May 2019

AbstractDomestic worker migration can profoundly reconfigure unpaid care arrangements within migrant households, often exacerbating gendered inequalities in providing and receiving care. While the International Labor Organization has led rights advocacy around migrant domestic work, there remains a dearth of attention to the relationship between feminized migration and unpaid care. In Sri Lanka, this policy space has been occupied by the Family Background Report: a series of regulations that reinforce maternal caregiving by restricting the migration of women with young children. An alternative “decent care” approach, involving investment in local care infrastructure, could yield multiple benefits while promoting a gender-inclusive decent work agenda.
Probabilistic Species Tree Distances: Implementing the Multispecies Coalescent to Compare Species Trees Within the Same Model-Based Framework Used to Estimate Them
Authors: Adams Richard H , Castoe Todd A 
Published: May 2019

AbstractDespite the ubiquitous use of statistical models for phylogenomic and population genomic inferences, this model-based rigor is rarely applied to post hoc comparison of trees. In a recent study, Garba et al. derived new methods for measuring the distance between two gene trees computed as the difference in their site pattern probability distributions. Unlike traditional metrics that compare trees solely in terms of geometry, these measures consider gene trees and associated parameters as probabilistic models that can be compared using standard information theoretic approaches. Consequently, probabilistic measures of phylogenetic tree distance can be far more informative than simply comparisons of topology and/or branch lengths alone. However, in their current form, these distance measures are not suitable for the comparison of species tree models in the presence of gene tree heterogeneity. Here, we demonstrate an approach for how the theory of Garba et al. (2018), which is based on gene tree distances, can be extended naturally to the comparison of species tree models. Multispecies coalescent (MSC) models parameterize the discrete probability distribution of gene trees conditioned upon a species tree with a particular topology and set of divergence times (in coalescent units), and thus provide a framework for measuring distances between species tree models in terms of their corresponding gene tree topology probabilities. We describe the computation of probabilistic species tree distances in the context of standard MSC models, which assume complete genetic isolation postspeciation, as well as recent theoretical extensions to the MSC in the form of network-based MSC models that relax this assumption and permit hybridization among taxa. We demonstrate these metrics using simulations and empirical species tree estimates and discuss both the benefits and limitations of these approaches. We make our species tree distance approach available as an R package called pSTDistanceR, for open use by the community.
Captive green iguana is a reservoir of diarrheogenicEscherichia colipathotypes
Authors: Bautista-Trujillo Gerardo Uriel , Gutiérrez-Miceli Federico Antonio , Mandujano-García Leonel , Oliva-Llaven María Angela , Ibarra-Martínez Carlos , Mendoza-Nazar Paula , Ruiz-Sesma Benigno , Tejeda-Cruz Carlos , Pérez-Vázquez Liset Candelaria , Pérez-Batrez Jesús Eduardo , Vidal Jorge E. , Gutiérrez-Jiménez Javier 
Published: May 2019

AbstractThe green iguana appears to be a reservoir for bacteria causing gastrointestinal infections in humans. The presence of diarrheogenicE. coli(DEC) pathotypes, however, has not been studied in this reptile. The aim of the current work was to investigate the prevalence of DEC in the intestines of 240 captive green iguanas, their phylogenetic groups, and the antibiotic susceptibility profile.E. colistrains were isolated from 41.7% of the intestinal content of green iguanas. DEC strains was identified in 25.9% of the screened population and were detected in the majority (62%, p=0.009] of those reptiles carryingE. colistrains. Among DEC strains, STEC strains carrying thestx1gene were the most prevalent pathotype isolated (38.7%), followed by EAEC and ETEC (27.4% each). Genetic markers of DEC strains belonging to the EHEC pathotype were not detected. More than a half of DEC strains were classified into the Clade I-II phylogroup (64.5%), followed by the phylogroup A (14.5%). The antibiotic susceptibility method demonstrated that a high proportion of DEC strains were resistance, or non-susceptible, to carbenicillin, amikacin, and ampicillin (85, 74, and 66%, respectively). We conclude that the green iguana kept in captivity is a reservoir of DEC strains bearing resistance to first-line antibiotics, including penicillins. Given the increase presence of the green iguana in Latin American households, these reptiles represent a potential source of transmission to susceptible humans and therefore a potential source of gastrointestinal disease.ImportanceLatin-American countries present a high burden of diarrheal disease. In this part of the world, besides common pathogens, diarrheal diseases are also caused by pathogenic types ofE. colireferred as “diarrheagenicE. coli” (DEC). While inhabitants of Latin American countries suffer of self-limiting diarrhea when infected with DEC, a main target of these strains are tourists from developed countries who are not exposed to DEC strains. Efforts are in place to decrease the burden of DEC-associated diarrheal disease. As such, this study investigated a potential reservoir of DEC strains that had been underestimated, the green iguana. These reptiles are very common in Latin American households and are found virtually everywhere in Mexico, Central and South America. We found that 25% of green iguanas carried DEC strains in their intestines. We also demonstrated a high prevalence of antibiotic resistance in these strains, posing a potential risk to humans.
Mixed-model admixture mapping identifies smoking-dependent loci of lung function in African Americans
Authors: Ziyatdinov Andrey , Parker Margaret M. , Vaysse Amaury , Beaty Terri H. , Kraft Peter , Cho Michael H. , Aschard Hugues 
Published: May 2019

AbstractAdmixture mapping has led to the discovery of many genes associated with differential disease risk by ancestry, highlighting the importance of ancestry-based approaches to association studies. However, the potential of admixture mapping in deciphering the interplay between genes and environment exposures has been seldom explored. Here, we performed a genome-wide screening of local ancestry-smoking interactions for five spirometric lung function phenotypes in 3,300 African Americans from the COPDGene study. To account for population structure and outcome heterogeneity across exposure groups, we developed a multi-component linear mixed model for mapping gene-environment interactions, and empirically showed its robustness and increased power. When applied to the COPDGene study, our approach identified two 11p15.2-3 and 2q37 loci, exhibiting local ancestry-smoking interactions at genome-wide significant level, that would have been missed by standard singlenucleotide polymorphism analyses. These two loci harbor the PARVA and RAB17 genes previously recognized to be involved in smoking behavior. Overall, our study provides the first evidence for potential synergistic effects between African ancestry and smoking on pulmonary function and underlines the importance of ethnic diversity in genetic studies.
Repurposing the aldose reductase inhibitor and diabetic neuropathy drug epalrestat for the congenital disorder of glycosylation PMM2-CDG
Authors: Iyer Sangeetha , Sam Feba S. , DiPrimio Nina , Preston Graeme , Verhejein Jan , Murthy Kausalya , Parton Zachary , Tsang Hillary , Lao Jessica , Morava Eva , Perlstein Ethan O. 
Published: May 2019

AbstractPhosphomannomutase 2 deficiency, or PMM2-CDG, is the most common congenital disorder of glycosylation affecting over 1,000 patients globally. There are no approved drugs that treat the symptoms or root cause of PMM2-CDG. In order to identify clinically actionable compounds that boost human PMM2 enzyme function, we performed a multi-species drug repurposing screen using a first-ever worm model of PMM2-CDG followed by PMM2 enzyme functional studies in PMM2-CDG patient fibroblasts. Drug repurposing candidates from this study, and drug repurposing candidates from a previously published study using yeast models of PMM2-CDG, were tested for their effect on human PMM2 enzyme activity in PMM2-CDG fibroblasts. Of the 20 repurposing candidates discovered in the worm-based phenotypic screen, 12 are plant-based polyphenols. Insights from structure-activity relationships revealed epalrestat, the only antidiabetic aldose reductase inhibitor approved for use in humans, as a first-in-class PMM2 enzyme activator. Epalrestat increased PMM2 enzymatic activity in four PMM2-CDG patient fibroblast lines with genotypes R141H/F119L, R141H/E139K, R141H/N216I and R141H/F183S. PMM2 enzyme activity gains range from 30% to 400% over baseline depending on genotype. Pharmacological inhibition of aldose reductase by epalrestat may shunt glucose from the polyol pathway to glucose-1,6-bisphosphate, which is an endogenous stabilizer and coactivator of PMM2 homodimerization. Epalrestat is a safe, oral and brain penetrant drug that was approved 27 years ago in Japan to treat diabetic neuropathy in geriatric populations. We demonstrate that epalrestat is the first small molecule activator of PMM2 enzyme activity with the potential to treat peripheral neuropathy and correct the underlying enzyme deficiency in a majority of pediatric and adult PMM2-CDG patients.
Soil and vegetation conditions changes following the different sand dune restoration measures on the Zoige Plateau
Authors: Luo Jiufu , Deng Dongzhou , Zhang Li , Zhu Xinwei , Chen Dechao , Zhou Jinxing 
Published: May 2019

AbstractRestoration of alpine sand dunes has been increasingly attracting the attention of ecologists due to their difficulty and importance among the mountain-river-forest-farmland-lake-grass system (referred as meta-ecosystem) restoration. Alpine sand dunes are suffered from unstable soil and lack of plants. Efficient restoration measures are vital to guide the sand dune restoration. Whether the engineering materials co-applied with seeding could achieve considerable restoration in such areas? Here, sandbag and wicker as environmental friendly materials combined with Elymus nutans seeding were implemented on the Zoige Plateau sand dune, comparing with the ‘control’ treatment that only seeding. We assessed the topsoil conditions by sampled the surface soil and measured the water capacity and nutrients. We also utilized interspecific relationship and population niche to analyse the plant community structure variances among different restoration measures. Results showed that the soil conditions got clearly improved in sandbag area than that in wicker area when compared with that in control area. The community in control area was the least structured, while the species showed the closest related in sandbag area. In addition, average population niche overlap showed a control (0.26) < wicker (0.32) < sandbag (0.39) ranking. Thus, we suggested that sandbag or wicker co-applied with indigenous grass seeding is a practical and quick restoration approach in alpine sand dunes, and the sandbag may surpasses the wicker. Moreover, soil amending measures including nutrient improvement, and microbial fertilizer addition may further accelerate sand dune restoration.
Perceived social norms, psychological capital and entrepreneurial intention among undergraduate students in Bukavu
Authors: Ephrem Akilimali Ndatabaye , Namatovu Rebecca , Basalirwa Edith Mwebaza 
Published: May 2019

PurposeEntrepreneurship is important for economic growth, through its role in the provision of employment. In the recent past, a number of African universities have developed entrepreneurship education courses to facilitate the growing demand for entrepreneurs in the market. An immediate outcome anticipated from entrepreneurship education is to increase entrepreneurial intention (EI) among the participants. Unfortunately, most of the entrepreneurship education in developing economies has not been linked to an increase in the EI of students. This paper thus proposes that it is when students possess high levels of psychological capital and perceive positive social norms that entrepreneurship education will lead to positive EI. The purpose of this paper is to establish the relationship between perceived social norms (PSN), psychological capital and EI of university students.Design/methodology/approachQuantitative data were collected through a self-administered questionnaire on a random sample of 196 final year entrepreneurship and business management students, from three universities in Bukavu (East of DRC). Structural equation modeling was used to test the research hypotheses.FindingsThe paper provides four main findings. First, PSN and psychological capital have a positive significant effect on EI. Second, PSN contribute more to this effect than psychological capital. Third, PSN make a positive and significant effect on psychological capital. Lastly, psychological capital positively mediates the relationship between PSN and EI.Research limitations/implicationsThis study could have benefited from a qualitative approach to have a more in-depth explanation of these relationships. The study is conducted amongst students who operate in a controlled environment. This may not reflect the actual behavior of entrepreneurs.Practical implicationsThis work provides cues of what entrepreneurship educators should consider as they recruit and train students. Specifically, the study highlights the importance of students’ psychological capital and positive social norms in transforming entrepreneurial education into intention.Originality/valueThis study adds value to knowledge by highlighting the mediating role of psychological capital on the relationship between PSN and EI.
The ethos and transformational nature of professional studies
Authors: Fergusson Lee , Van Der Laan Luke , White Craig , Balfour June 
Published: May 2019

Purpose
The purpose of this paper is to explore the work-based learning (WBL) ethos of a professional studies doctoral program, a higher degree by research program implemented in Australia.

Design/methodology/approach
This is a preliminary case study of one higher degree by research program and two doctoral candidates participating in the program to explore the ethos and outcomes of the program.

Findings
The program has sought to develop a different type of higher education ethos, one characterized by an open-door communications policy, a critical friend philosophy, an emphasis on teamwork, pro tem supervision and a new model for doctoral supervision, self-designed work-based projects, self-directed research programs and the development of professional identity.

Originality/value
The characteristics and contributions of WBL programs at the doctoral level have been well documented in the academic literature, but the unique ethos, if there is one, of such programs has yet to be fully examined. This study goes some of the way to answering the question of whether such programs have a unique ethos and if so what are its features and how might it contribute to student development.
Numerical study and POD snapshot analysis of flow characteristics for pulsating turbulent opposing jets
Authors: H. Bagheri Morteza , Esmailpour Kazem , Hoseinalipour Seyyed Mostafa , Mujumdar Arun S. 
Published: May 2019

Purpose
The purpose of this study is to investigate the coherent structures of pulsed opposing jets by large eddy simulation (LES) model and proper orthogonal decomposition (POD) snapshot method. Flow pulsation as an active flow control method is considered for the enhancement of transport phenomena in impinging jets. The effect of flow pulsation parameters such as pulsation signal shape and frequency on the vortical coherent structures, the energy content of primary modes and their variation are studied numerically.

Design/methodology/approach
In this study, flow field of turbulent pulsating opposing jets has been simulated using LES. The result of the simulation in different time steps (snapshots) are stored and POD is applied on the snapshots. In this study, the POD method and calculation of spatial modes has been done using OpenFOAM, and time coefficients have been calculated using a MATLAB code.

Findings
The results of this study show that the flow excitation has a great effect on the coherent structure formation and the energy containment of fundamental modes of the flow. When the flow was excited by a harmonic sinusoidal or step function, the turbulent kinetic energy accumulated in the set of primary modes. On the other hand, the pulsed opposing jets had more regularity compared to the steady jets. The shapes, patterns and energy values of dominant modes depended on the inlet pulsation signal. An increase in pulsation frequency leads to an augmentation in energy content of the primary modes.

Research limitations/implications
The predictions may be extended to include various pulsation conditions such as: various amplitudes, Reynolds number and aspect ratio.

Practical implications
The results of this study are a valuable source of information for active control of transport phenomena in opposing jet configurations which is used in different industrial applications such as cooling, combustion, reactors, heating and drying processes.

Originality/value
In this study, the coherent structures and energy content of primary modes was studied for the first time by LES model and POD snapshot method and a comprehensive discussion on numerical results is provided.
A recommended closed-loop assessment of learning outcomes process for hospitality programs
Authors: Reich Allen Z. , Collins Galen R. , DeFranco Agnes L. , Pieper Suzanne L. 
Published: May 2019

Purpose
Because of the increasingly higher expectations of accrediting organizations, calls for greater accountability from state governments, and students’ demand for an education that prepares them for a career, most hospitality programs are now required to have an effective assessment of learning outcomes process. The increasing popularity of the assessment of learning outcomes process is viewed as highly positive because it can be considered as best practices in higher education. The paper aims to discuss this issue.

Design/methodology/approach
This is Part 1 of a two-part article that provides an overview of the justifications for implementing an assessment of learning outcomes process, the steps that were developed by two hospitality programs and the experiences of the two programs during implementation of the seven steps. Part 1 includes foundational principles of the process and the first three of the seven steps.

Findings
The steps in a closed-loop assessment of learning outcomes process are relatively detailed; however, because of changes in expectations of stakeholders and the requirements of accreditors, they are now mandatory for most hospitality programs. Therefore, the choice is not whether to implement them, but when to implement them. From a competitive standpoint, it is to the program’s advantage to begin as soon as possible. Another factor to consider is that the implementation of an effective closed-loop assessment of learning outcomes process will take several years to complete.

Originality/value
This paper is presenting a critical view of one of, if not the most important concepts in higher education, the closed-loop assessment of learning outcomes process. Hopefully, the information on the process that is provided and the experiences of the two programs can shorten the learning curve for other hospitality programs.