bluuebunny/crossref_metadata_2025_split huggingface dataset:
Unexpectedly rapid evolution of mandibular shape in hominins
Authors: Raia P. , Boggioni M. , Carotenuto F. , Castiglione S. , Di Febbraro M. , Di Vincenzo F. , Melchionna M. , Mondanaro A. , Papini A. , Profico A. , Serio C. , Veneziano A. , Vero V. A. , Rook L. , Meloro C. , Manzi G. 
Published: May 2018

AbstractMembers of the hominins – namely the so-called ‘australopiths’ and the species of the genus Homo – are known to possess short and deep mandibles and relatively small incisors and canines. It is commonly assumed that this suite of traits evolved in early members of the clade in response to changing environmental conditions and increased consumption of though food items. With the emergence of Homo, the functional meaning of mandible shape variation is thought to have been weakened by technological advancements and (later) by the control over fire. In contrast to this expectation, we found that mandible shape evolution in hominins is exceptionally rapid as compared to any other primate clade, and that the direction and rate of shape change (from the ape ancestor) are no different between the australopiths and Homo. We deem several factors including the loss of honing complex, canine reduction, and the acquisition of different diets may have concurred in producing such surprisingly high evolutionary rates. This study reveals the evolution of mandibular shape in hominins has strong morpho-functional and ecological significance attached.
The relation between natural variations in ocean heat uptake and global mean surface temperature anomalies in CMIP5
Authors: Drijfhout Sybren 
Published: May 2018

AbstractIt is still unclear whether a hiatus period arises due to a vertical redistribution of ocean heat content (OHC) without changing ocean heat uptake (OHU), or whether the increasing radiative forcing is associated with an increase in OHU when global mean surface temperature (GMST) rise stalls. By isolating natural variability from forced trends and performing a more precise lead-lag analysis, we show that in climate models TOA radiation and OHU do anti-correlate with natural variations in GMST, when GMST leads or when they coincide, but the correlation changes sign when OHU leads. Surface latent and sensible heat fluxes always force GMST-variations, whilst net surface longwave and solar radiation fluxes have a damping effect, implying that natural GMST-variations are caused by oceanic heat redistribution. In the models an important trigger for a hiatus period on decadal timescales is increased reflection of solar radiation, by increased sea-ice cover over deep-water formation areas. On inter-annual timescales, reflection of solar radiation in the tropics by increased cloud cover associated with La Niña is most important and the subsequent reduction in latent heat release becomes the dominant cause for a hiatus.
A single amino acid substitution in the Bombyx-specific mucin-like membrane protein causes resistance to Bombyx mori densovirus
Authors: Ito Katsuhiko , Kidokoro Kurako , Katsuma Susumu , Sezutsu Hideki , Uchino Keiro , Kobayashi Isao , Tamura Toshiki , Yamamoto Kimiko , Mita Kazuei , Shimada Toru , Kadono-Okuda Keiko 
Published: May 2018

AbstractBombyx mori densovirus type 1 (BmDV) is a pathogen that causes flacherie disease in the silkworm. The absolute nonsusceptibility to BmDV among certain silkworm strains is determined independently by two genes, nsd-1 and Nid-1. However, neither of these genes has been molecularly identified to date. Here, we isolated the nsd-1 gene by positional cloning and characterized the properties of its product, NSD-1. Sequence and biochemical analyses revealed that this gene encodes a Bombyx-specific mucin-like glycoprotein with a single transmembrane domain. The NSD-1 protein was specifically expressed in the larval midgut epithelium, the known infection site of BmDV. Sequence analysis of the nsd-1 gene from 13 resistant and 12 susceptible strains suggested that a specific arginine residue in the extracellular tail of the NSD-1 protein was common among susceptible strains. Germline transformation of the susceptible-type nsd-1 (with a single nucleotide substitution) conferred partial susceptibility to resistant larvae, indicating that the +
nsd-1
gene is required for the susceptibility of B. mori larvae to BmDV and the susceptibility is solely a result of the substitution of a single amino acid with arginine. Taken together, our results provide striking evidence that a novel membrane-bound mucin-like protein functions as a cell-surface receptor for a densovirus.
Calretinin Participates in Regulating Steroidogenesis by PLC-Ca2+-PKC Pathway in Leydig Cells
Authors: Xu Wendan , Zhu Qian , Liu Shan , Dai Xiaonan , Zhang Bei , Gao Chao , Gao Li , Liu Jiayin , Cui Yugui 
Published: May 2018

AbstractCalretinin, a Ca2+-binding protein, participates in many cellular events. Our previous studies found the high expression of calretinin in testicular Leydig cells. In this study, (MLTC-1 cells were infected with LV-calb2, R2C cells with LV-siRNA-calb2. The primary mouse Leydig cells were also used to confirm those data from cell lines. Testosterone level was significantly higher in the MLTC-1 cells with over-expressed calretinin than in the control, while progesterone was lower in the R2C cells in which down-regulated calretinin. The expressions of StAR changed in synchrony with hormones. Cytoplasmic Ca2+ level was significantly increased when calretinin was over-expressed. When MLTC-1 cells were infected with LV-calb2 and then stimulated using Clopiazonic, a Ca2+-releasing agent, testosterone was significantly increased. Interestingly, the expression levels of PLC, p-PKCµ (PKD), p-MARCKS and CREB, were significantly increased in the MLTC-1 cells with over-expressed calretinin, while PLC, p-PKD, p-MARCKS, MARCKS and CREB were decreased in the R2C cells with down-regulated calretinin. We also observed the increased expression of calretinin up-regulated testosterone production and the expressions of StAR and PLC in primary mouse Leydig cells. So, calretinin as a Ca2+-binding protein participates in the regulation of steroidogenesis via the PLC-Ca2+-PKC pathway in Leydig cells.
Spin valley and giant quantum spin Hall gap of hydrofluorinated bismuth nanosheet
Authors: Gao Heng , Wu Wei , Hu Tao , Stroppa Alessandro , Wang Xinran , Wang Baigeng , Miao Feng , Ren Wei 
Published: May 2018

AbstractSpin-valley and electronic band topological properties have been extensively explored in quantum material science, yet their coexistence has rarely been realized in stoichiometric two-dimensional (2D) materials. We theoretically predict the quantum spin Hall effect (QSHE) in the hydrofluorinated bismuth (Bi2HF) nanosheet where the hydrogen (H) and fluorine (F) atoms are functionalized on opposite sides of bismuth (Bi) atomic monolayer. Such Bi2HF nanosheet is found to be a 2D topological insulator with a giant band gap of 0.97 eV which might host room temperature QSHE. The atomistic structure of Bi2HF nanosheet is noncentrosymmetric and the spontaneous polarization arises from the hydrofluorinated morphology. The phonon spectrum and ab initio molecular dynamic (AIMD) calculations reveal that the proposed Bi2HF nanosheet is dynamically and thermally stable. The inversion symmetry breaking together with spin-orbit coupling (SOC) leads to the coupling between spin and valley in Bi2HF nanosheet. The emerging valley-dependent properties and the interplay between intrinsic dipole and SOC are investigated using first-principles calculations combined with an effective Hamiltonian model. The topological invariant of the Bi2HF nanosheet is confirmed by using Wilson loop method and the calculated helical metallic edge states are shown to host QSHE. The Bi2HF nanosheet is therefore a promising platform to realize room temperature QSHE and valley spintronics.
Unbiased estimation of an optical loss at the ultimate quantum limit with twin-beams
Authors: Losero Elena , Ruo-Berchera Ivano , Meda Alice , Avella Alessio , Genovese Marco 
Published: May 2018

AbstractLoss measurements are at the base of spectroscopy and imaging, thus permeating all the branches of science, from chemistry and biology to physics and material science. However, quantum mechanics laws set the ultimate limit to the sensitivity, constrained by the probe mean energy. This can be the main source of uncertainty, for example when dealing with delicate systems such as biological samples or photosensitive chemicals. It turns out that ordinary (classical) probe beams, namely with Poissonian photon number distribution, are fundamentally inadequate to measure small losses with the highest sensitivity. It is known that quantum-correlated pair of beams, named “twin-beam state”, allows surpassing this classical limit. Here we demonstrate they can reach the ultimate sensitivity for all energy regimes (even less than one photon per mode) with the simplest measurement strategy. One beam of the pair addresses the sample, while the second one is used as a reference to compensate both for classical drifts and for fluctuation at the most fundamental quantum level. This capability of selfcompensating for unavoidable instability of the sources and detectors allows also to strongly reduce the bias in practical measurement. Moreover, we report the best sensitivity per photon ever achieved in loss estimation experiments.
Expressional analysis of disease-relevant signalling-pathways in primary tumours and metastasis of head and neck cancers
Authors: Goesswein Dorothee , Habtemichael Negusse , Gerhold-Ay Aslihan , Mazur Johanna , Wünsch Désirée , Knauer Shirley K. , Künzel Julian , Matthias Christoph , Strieth Sebastian , Stauber Roland H. 
Published: May 2018

AbstractHead and neck squamous cell carcinoma (HNSCC) often metastasize to lymph nodes resulting in poor prognosis for patients. Unfortunately, the underlying molecular mechanisms contributing to tumour aggressiveness, recurrences, and metastasis are still not fully understood. However, such knowledge is key to identify biomarkers and drug targets to improve prognosis and treatments. Consequently, we performed genome-wide expression profiling of 15 primary HNSSCs compared to corresponding lymph node metastases and non-malignant tissue of the same patient. Differentially expressed genes were bioinformatically exploited applying stringent filter criteria, allowing the discrimination between normal mucosa, primary tumours, and metastases. Signalling networks involved in invasion contain remodelling of the extracellular matrix, hypoxia-induced transcriptional modulation, and the recruitment of cancer associated fibroblasts, ultimately converging into a broad activation of PI3K/AKT-signalling pathway in lymph node metastasis. Notably, when we compared the diagnostic and prognostic value of sequencing data with our expression analysis significant differences were uncovered concerning the expression of the receptor tyrosine kinases EGFR and ERBB2, as well as other oncogenic regulators. Particularly, upregulated receptor tyrosine kinase combinations for individual patients varied, implying potential compensatory and resistance mechanisms against specific targeted therapies. Collectively, we here provide unique transcriptional profiles for disease predictions and comprehensively analyse involved signalling pathways in advanced HNSCC.
Colon Cancer-Upregulated Long Non-Coding RNA lincDUSP Regulates Cell Cycle Genes and Potentiates Resistance to Apoptosis
Authors: Forrest Megan E. , Saiakhova Alina , Beard Lydia , Buchner David A. , Scacheri Peter C. , LaFramboise Thomas , Markowitz Sanford , Khalil Ahmad M. 
Published: May 2018

AbstractLong non-coding RNAs (lncRNAs) are frequently dysregulated in many human cancers. We sought to identify candidate oncogenic lncRNAs in human colon tumors by utilizing RNA sequencing data from 22 colon tumors and 22 adjacent normal colon samples from The Cancer Genome Atlas (TCGA). The analysis led to the identification of ~200 differentially expressed lncRNAs. Validation in an independent cohort of normal colon and patient-derived colon cancer cell lines identified a novel lncRNA, lincDUSP, as a potential candidate oncogene. Knockdown of lincDUSP in patient-derived colon tumor cell lines resulted in significantly decreased cell proliferation and clonogenic potential, and increased susceptibility to apoptosis. The knockdown of lincDUSP affects the expression of ~800 genes, and NCI pathway analysis showed enrichment of DNA damage response and cell cycle control pathways. Further, identification of lincDUSP chromatin occupancy sites by ChIRP-Seq demonstrated association with genes involved in the replication-associated DNA damage response and cell cycle control. Consistent with these findings, lincDUSP knockdown in colon tumor cell lines increased both the accumulation of cells in early S-phase and γH2AX foci formation, indicating increased DNA damage response induction. Taken together, these results demonstrate a key role of lincDUSP in the regulation of important pathways in colon cancer.
Anomalous Scaling of Gene Expression in Confined Cell-Free Reactions
Authors: Sakamoto Ryota , Noireaux Vincent , Maeda Yusuke T. 
Published: May 2018

AbstractCellular surface breaks the symmetry of molecular diffusion across membrane. Here, we study how steric interactions between the surface and the bulk of cell-sized emulsion droplets alters gene expression emulated by a cell-free transcription/translation (TXTL) system. The concentration of synthesized reporter proteins in droplets of radius R shows an anomalous geometric scaling of R4 different from the expected size-dependence of R3. Given that TXTL becomes less efficient at thin surface layer, a mathematical model explains the anomalous size-dependence found in experiment. The surface of cell-sized compartment can thus play a regulatory role for cell-free gene expression.
Radioprotective effect of Date syrup on radiation- induced damage in Rats
Authors: Abou-Zeid Shimaa M. , EL-bialy Badr E. , EL-borai Nermeen B. , AbuBakr Huda O. , Elhadary Abdel Monsef A. 
Published: May 2018

AbstractIonizing radiation has cytotoxic and genotoxic effects caused mainly by the oxidative damage induced by free radical release. The need for radioprotectives is increasing to protect normal tissues during radiotherapy. In the present study, we investigated the radioprotective effect of Date syrup in rats subjected to whole body radiation at 6 Gy through biochemical, molecular and histopathological analysis. Significant elevations were recorded in the activities of serum ALT, AST, ALP and LDH and in the levels of all lipid profiles parameters, while the level of HDL-C was reduced. The concentration of liver MDA was elevated with depletion of hepatic glutathione (GSH) and catalase. DNA damage was evidenced by increased DNA strand breakage and DNA-protein crosslinks. Significant elevations were observed in the expression of liver TNF-α and serum activity of matrix metalloproteinase (MMP-9). Pretreatment of rats with Date syrup ameliorated the tissue damage induced by radiation as evidenced by the improvement of liver function, antioxidant status and reduction of DNA damage. Besides, liver TNF-α expression and serum MMP-9 activity were reduced. In conclusion, Date syrup could alleviate the toxic effects of ionizing radiation and thus is useful as a radioprotective in radiotherapy regimen.
Integrated analysis highlights APC11 protein expression as a likely new independent predictive marker for colorectal cancer
Authors: Drouet Youenn , Treilleux Isabelle , Viari Alain , Léon Sophie , Devouassoux-Shisheboran Mojgan , Voirin Nicolas , de la Fouchardière Christelle , Manship Brigitte , Puisieux Alain , Lasset Christine , Moyret-Lalle Caroline 
Published: May 2018

AbstractAfter a diagnosis of colorectal cancer (CRC), approximately 50% of patients will present distant metastasis. Although significant progress has been made in treatments, most of them will die from the disease. We investigated the predictive and prognostic potential of APC11, the catalytic subunit of APC/C, which has never been examined in the context of CRC. The expression of APC11 was assessed in CRC cell lines, in tissue microarrays (TMAs) and in public datasets. Overexpression of APC11 mRNA was associated with chromosomal instability, lymphovascular invasion and residual tumor. Regression models accounting for the effects of well-known protein markers highlighted association of APC11 protein expression with residual tumor (odds ratio: OR = 6.51; 95% confidence intervals: CI = 1.54–27.59; P = 0.012) and metastasis at diagnosis (OR = 3.87; 95% CI = 1.20–2.45; P = 0.024). Overexpression of APC11 protein was also associated with worse distant relapse-free survival (hazard ratio: HR = 2.60; 95% CI = 1.26–5.37; P = 0.01) and worse overall survival (HR = 2.69; 95% CI = 1.31–5.51; P = 0.007). APC11 overexpression in primary CRC thus represents a potentially novel theranostic marker of metastatic CRC.
Drivers of demographic decline across the annual cycle of a threatened migratory bird
Authors: Wilson Scott , Saracco James F. , Krikun Richard , Flockhart D. T. Tyler , Godwin Christine M. , Foster Kenneth R. 
Published: May 2018

AbstractMigratory species are rapidly declining but we rarely know which periods of the annual cycle are limiting for most species. This knowledge is needed to effectively allocate conservation resources to the periods of the annual cycle that best promote species recovery. We examined demographic trends and response to human footprint for Canada warblers (Cardellina canadensis), a threatened Neotropical migrant, using range-wide data (1993–2016) from the Monitoring Avian Productivity and Survivorship (MAPS) program on the breeding grounds. Declines in abundance were steepest in the eastern breeding region, followed by the western region. Breeding productivity did not decline in any region. In contrast, we observed declining recruitment in all regions, low apparent survival in the east and west, and a decline in apparent survival in the east. Abundance declined with increasing disturbance around MAPS stations. Between 1993 and 2009, the human footprint index on the breeding range increased by 0.11% in contrast to a 14% increase on the wintering range. Landscape-scale disturbance on the breeding grounds may influence abundance in some regions; however, the observed trends in demography and footprint suggests limitation during the non-breeding period as the likely driver of overall declines, particularly for eastern populations.
Replication study of polymorphisms associated with response to methotrexate in patients with rheumatoid arthritis
Authors: López-Rodríguez Rosario , Ferreiro-Iglesias Aida , Lima Aurea , Bernardes Miguel , Pawlik Andrzej , Paradowska-Gorycka Agnieszka , Świerkot Jerzy , Slezak Ryszard , Dolžan Vita , González-Álvaro Isidoro , Narváez Javier , Cáliz Rafael , Pérez-Pampín Eva , Mera-Varela Antonio , Vidal-Bralo Laura , Acuña Ochoa José Gorgonio , Conde Carmen , Gómez-Reino Juan J. , González Antonio 
Published: May 2018

AbstractAbout 70 genetic studies have already addressed the need of biomarkers to predict the response of patients with rheumatoid arthritis (RA) to methotrexate (MTX) treatment. However, no genetic biomarker has yet been sufficiently validated. Here, we aimed to replicate a selection of 25 SNPs in the largest collection of patients up to date, which consisted of 915 patients treated with MTX. The change in disease activity (measured as ΔDAS28) from baseline was considered the primary outcome. In addition, response according to widely used criteria (EULAR) was taken as secondary outcome. We considered consistency between outcomes, P values accounting for the number of SNPs, and independence from potential confounders for interpretation of the results. Only the rs1801394 SNP in MTRR fulfilled the high association standards. Its minor allele was associated with less improvement than the major allele according to ΔDAS28 (p = 0.0016), and EULAR response (p = 0.004), with independence of sex, age, baseline DAS28, smoking, seropositivity, concomitant corticosteroid use or previous treatments. In addition, previous evidence suggests the association of this SNP with response to MTX in another autoimmune disease, juvenile idiopathic arthritis, and with high intracellular folate levels, which could contribute to poor response.
Low-Yellowing Phosphor-in-Glass for High-Power Chip-on-board White LEDs by Optimizing a Low-Melting Sn-P-F-O Glass Matrix
Authors: Yoon Hee Chang , Yoshihiro Kouhara , Yoo Heeyeon , Lee Seung Woo , Oh Ji Hye , Do Young Rag 
Published: May 2018

AbstractWe introduce a low-melting-point (MP) Sn-P-F-O glass ceramic material into the phosphor-in-glass (PIG) material to realize an ‘on-chip’ chip-on-board (COB) type of phosphor-converted (pc) white light-emitting diode (WLED) with green (BaSr)2SiO4:Eu2+ and red (SrCa)AlSiN3:Eu2+ (SCASN) phosphors. The optimum Sn-P-F-O-based ceramic components can be sintered into the glass phase with a facile one-step heating process at 285 °C for 1 min. Specifically, these soft-fabrication conditions can be optimized to minimize the degradation of the luminescent properties of the red SCASN phosphor as well as the green silicate phosphor in PIG-based white COB-type pc-LEDs owing to the low thermal loss of the phosphors at low fabrication temperatures below 300 °C. Moreover, the constituents of the COB package, in this case the wire bonding and plastic exterior, can be preserved simultaneously from thermal damage. That is, the low sintering temperature of the glass ceramic encapsulant is a very important factor to realize excellent optical qualities of white COB LEDs. The optical performances of low-MP Sn-P-F-O-based PIG on-chip COB-type pc-WLEDs exhibit low yellowing phenomena, good luminous efficacy of 70.9–86.0 lm/W, excellent color rendering index of 94–97 with correlated color temperatures from 2700 to 10000 K, and good long-term stability.
Clinical evaluation of silicone gel in the treatment of cleft lip scars
Authors: Chang Chun-Shin , Wallace Christopher Glenn , Hsiao Yen-Chang , Huang Jung-Ju , Chen Zung-Chung , Chang Chee-Jen , Lo Lun-Jou , Chen Philip Kuo-Ting , Chen Jyh-Ping , Chen Yu-Ray 
Published: May 2018

AbstractUpper lip scars are at risk of hypertrophy. Our center therefore uses microporous tape and silicone sheeting for postoperative scar care following cleft lip repair. However, some babies have previously ingested their silicone sheeting, which has the potential for respiratory compromise or gastrointestinal obstruction. Self-dry silicone gel is reportedly also effective for preventing hypertrophic scars. Hence, we sought to test whether silicone gel, which cannot be ingested whole, might be non-inferior to silicone sheeting for controlling against upper lip scar hypertrophy. This was a mixed prospective and retrospective case-controlled clinical trial involving patients undergoing unilateral cleft lip repair, 29 of whom received standard postoperative silicone sheeting (control group) and another 33 age-matched consecutive patients who received self-dry silicone instead. The Vancouver scar scale, visual analogue scale and photographically assessed scar width assessments were the same in both groups at six months after surgery. In conclusion, silicone gel appears to be non-inferior to silicone sheeting for postoperative care of upper lip scars as judged by scar quality at six months, but silicone sheeting has the safety disadvantage that it can be swallowed whole by babies. It is thus recommended that silicone gel be used for upper lip scar management in babies.