bluuebunny/crossref_metadata_2025_split huggingface dataset:
213 Transcriptome profiling of olfactory epithelium in normal cycling and acyclic gilts
Authors: Nonneman Dan J , Dickey Aaron M , Lents Clay A 
Published: July 2019

Abstract
A significant proportion of gilts that enter the herd never farrow a litter and are culled because of anestrus or failure to conceive. Genome-wide association studies for pubertal traits have identified loci involved in neuronal and olfactory pathways, and olfaction is critical for expression of reproductive behavior in mammalian females. We evaluated major olfactory epithelium (MOE) for differential gene expression in nonpubertal, behavioral anestrus and normal cycling early follicular and luteal phase gilts (n = 8/group; average age of 259 days). An average of 50 million paired-end RNA-seq reads were collected from each of the 32 RNA libraries and mapped to Sscrofa 11.1. Differential gene expression was determined using DESeq2. A total of 18,484 genes were expressed with a mean normalized expression value greater than 5. Only four genes were differentially expressed between nonpubertal or behavioral anestrus MOE and their cycling controls (early follicular and luteal, respectively). Comparing cycling follicular and luteal phase gilts showed that 1146 genes were more highly expressed in MOE from follicular phase gilts, whereas 1351 genes were more highly expressed in MOE from luteal phase gilts. Pathways for transmembrane receptor protein tyrosine kinase/growth factor signaling, cell junction and epithelium development were overrepresented in follicular phase MOE and cell cycle, chromatin remodeling, inflammatory response and sensory perception were overrepresented in luteal phase MOE. While 1348 locus IDs were identified for olfactory receptors in MOE, only 160 were expressed at an appreciable level (base mean > 5) in MOE and 16 were more highly expressed in MOE from luteal phase than follicular phase gilts. While few genes were differentially expressed in MOE between prepubertal and anestrus gilts and cycling gilts at the same ovarian stage, the comparison between ovarian stages indicates that MOE gene expression is under hormonal control. USDA is an equal opportunity provider and employer.
221 Optimizing sugar inclusion in high roughage diets for growing cattle
Authors: VanDerWal Allison , Zeltwanger Josh , DiCostanzo Alfredo 
Published: July 2019

Abstract
Producers wishing to background cattle may not have access to grain in certain regions of the country or when grain price is high. Sugar sources may replace grain under these circumstances. The objective of this study was to determine optimum sugar concentration based on daily gain and feed conversion in high-forage diets fed to growing cattle. Ninety-two Angus crossbred (Ranch 1) steers (n = 60; 339 + 11 kg BW) and heifers (n = 32; 309 + 14 kg BW), and 89 Red Angus crossbred (Ranch 2) steers (338 + 16 kg BW) were randomly allocated (5 to 7 hd/pen) within ranch and sex to one of 15 pens in each of two (north or south side) locations within a deep bedded confinement feedlot. Target dietary treatments were designed to contain supplemental sugar inclusion of 0%, 3.5%, 7% or 10.5% (8, 7, 8 or 7 replicate pens in each treatment, respectively) using a molasses-based supplement containing 56.8% sugar and 7.4% urea. Diets were comprised (DM basis) of hay (12%), corn silage, dry distillers grains (14.5%), dry rolled corn and a liquid supplement (5%); corn grain and corn silage inclusion varied from 21% to 32% and from 29% to 36%, respectively, to accommodate supplemental sugar. This resulted in dietary sugar concentrations of 4.3%, 7.3%, 10.3% and 13.3% or 0%, 3%, 6%, and 9%, respectively, as supplemental sugar. As concentration of sugar increased, DMI increased linearly (P < 0.006) with no change in ADG (P = 0.22) resulting in linearly decreasing feed conversion efficiency (P = 0.032). Iterated ME concentration of supplemental sugar was similar to that of corn grain (P > 0.06). Given the appropriate conditions, sugar may replace dry rolled corn and corn silage in high roughage diets for growing cattle.
331 Economic comparison of march and may calving systems in the nebraska sandhillls
Authors: Broadhead Devin L , Stockton Matt , Erickson McKay , Musgrave Jackie A , Funston Rick N 
Published: July 2019

Abstract
A 3-yr study evaluated the economic differences between a March and May calving production system of crossbred beef cows and their offspring from the Gudmundsen Sandhills Laboratory, Whitman, NE. Both herds were treated as a common when not in treatment period. Adjusted calf weaning BW was higher (P) for March Calves (226.4 ± 1.1 kg vs 193.4 ± 2 kg). Pregnancy rates (89% vs 91%) were similar through both systems. The stochastic economic model used for the analysis was based on 9 yr of USDA AMS data. The model accounts for most assumptions within each system, including all labor, cull and replacement cow costs and feed costs. This analysis was on the total calf costs, total pair feed costs and average market net return at weaning. March systems wintered on hay had a positive net return 2 out of the 9 yr (Average of -$88.76/calf) and on cornstalks 8 out of 9 years in Dawson County, NE (Average of $62.75/calf). March systems on winter range feed 0.41 kg DM/(cow • d) of supplement had a positive net return 4 out of the 9 yrs (Average of $25.23/calf). The May system, no matter the treatments of range or meadow and with or without supplement, had a positive net return 2 out of the 9 years (Average of $-65.77 and $-83.90/calf). Within this analysis, even with the input costs being higher for March, the net return was still greater in a March vs May system. Further analysis will be done on different trts within each system.
330 Identification and genomic characterization of two novel strains of Prevotella albensis as starch utilizers in the rumen of beef cows
Authors: Bandarupalli Venkata , St- Pierre Benoit 
Published: July 2019

Abstract
In cattle fed concentrate diets, rumen amylolytic bacteria digest starch into glucose, which is metabolized for growth. Since metagenomics studies have revealed that uncharacterized ruminal amylolytic bacteria far outnumber known starch utilizers, we have been pursing the identification of novel ruminal amylolytic bacteria. The same Operational Taxonomic Unit (OTU) was enriched independently from the rumen fluid of two beef cows after culturing with starch. Since this identification was performed using the V1–V3 region of the 16S rRNA gene, a metagenomic analysis was conducted to determine whether this OTU represented the same strain or different strains of the same species. A total of 9.25 and 9.16 million sequence reads were respectively generated from a select enriched starch culture from each cow, which had a relative abundance for this OTU of 67.9% and 74.0%, respectively. Contigs were assembled using the publicly available software ABySS, with contigs of at least 2kb in length used for further analysis. Of the enzymes identified by gene annotation of these contigs (using a combination of the online tools RAST and BLASTp), the presence of genes encoding α-amylase and lactate dehydrogenase enzymes further supported this OTU as corresponding to a starch utilizer. The alpha-amylase isoforms from the two rumens differed in amino acid length (538 vs 625) and sequence, with their respective closest affiliation being to an uncultured species of Lachnospiraceae (51% amino acid identity) and to Prevotella albensis (95% amino acid identity), respectively. The lactate dehydrogenase isoforms were also found to be different in length (348 aa vs 335 aa) and sequence (100% amino acid identity to Lactobacillus mucosae and 99% to an uncultured species of the genus Olsonella, respectively). These and other gene comparisons together suggest that two strains of the same starch-utilizing OTU have been identified in the rumen of beef cows.
333 Effects of ruminally-protected lysine and Megasphaera elsdenii on performance and carcass characteristics of finishing cattle
Authors: Veloso Vanessa de Aguiar , Horton Lucas , Baker Adrian , Aperce Celine , Drouillard James 
Published: July 2019

Abstract
Crossbred steers (n = 448; 352 ± 25 kginitial body weight) were used to evaluate impact of Megasphaera elsdenii (ME; Lactipro advance, MS Biotec, Wamego, KS), alone or in combination with ruminally-protected lysine (RPL; USA Lysine, Kemin Industries, Inc., USA), on performance and carcass traits of finishing steers in a randomized complete block design. Steers were blocked by initial body weight and randomly assigned within block to one of 64 pens (7 steers/pen) in a 2 x 2 factorial treatment arrangement. Factors consisted of RPL (0 or 40 g/steer daily) and two step-up regimens (21-d without ME or 10-d with ME). Four step-up diets were used to transition cattle to finishing diets containing (dry basis) 60% steam flaked corn, 7% wheat straw, 30% sweet bran, and supplement. Diets were fed once daily ad libitum. Freeze-dried ME was re-hydrated and dosed orally (1x1010 CFU/steer) on d 1, and top-dressed (1x107 CFU/steer daily) onto diets daily thereafter. Data were analyzed using a mixed model with ME, RPL and the interaction as fixed effects, and block as the random effect. There were no interactions between ME and RPL (P > 0.05). Steers given ME consumed 16% less roughage compared to their counterparts without (P < 0.05), but gain, dry matter intake, and gain:feed were similar (P > 0.10). Administering ME tended to increase percentage of USDA Prime carcasses (0.5 vs 2.7%; P < 0.06). Feeding RPL did not affect feedlot performance, carcass weight, longissimus muscle area, marbling score, 12th-rib fat thickness, or liver abscess incidence (P > 0.10), but tended to increase yield grade (P < 0.07). In conclusion, use of Megasphaera elsdenii with an accelerated step-up program yielded feedlot performance and carcass traits comparable to those of cattle adapted with a conventional, 21-d step-up program, but supplemental lysine was without effect.
337 Effect of adding urea to finishing diets containing two different inclusions of distillers grains on steer performance and carcass characteristics
Authors: Boyd Bradley M , MacDonald James C , Luebbe Matthew , Erickson Galen E 
Published: July 2019

Abstract
Crossbred beef steers (n = 384) were utilized to evaluate the effects of adding urea to a dry rolled corn based finishing diet containing low inclusions of wet distillers grains plus solubles (WDGS). Treatments were set up as a 2 × 4 factorial arrangement with factors consisting of WDGS inclusion at either 12 or 20% of diet dry matter (DM) and urea inclusion at either 0, 0.4, 0.8, 1.2% of diet DM. Cattle were blocked and stratified by initial body weight (BW) and assigned randomly to treatment. The heavy block of cattle were harvested after 160 d on feed (DOF) and the middle and light blocks of cattle were harvested after 179 DOF. The model include the fixed effects of weight block, WDGS inclusion, urea inclusion, and their interactions. There were no significant interactions (P > 0.12) observed between WDGS inclusion and urea inclusion in the diet; therefore, only main effects are discussed. Increasing inclusion of WDGS improved (P < 0.04) carcass adjusted ADG and G:F. Dry matter intake was reduced (P = 0.04) with increasing WDGS inclusions. Fat thickness was increased (P = 0.04) with increased WDGS inclusion; however, no other carcass characteristics were significantly affected by WDGS inclusion. Cubic effects of urea inclusion were observed (P < 0.05) for HCW, final BW, ADG, and DMI on a carcass adjusted and live BW basis; however, cubic effects with increasing urea inclusion are of minimal biological relevance. There were no linear or quadratic responses (P > 0.11) for increasing urea inclusion in the diet for performance measurements or carcass characteristics. Increasing WDGS inclusion in the diet improved performance. Added urea in the diet had minimal impact on performance, regardless of wet distillers grains plus solubles inclusion.
348 Effects of supplemental copper, zinc, and manganese source on growth performance and carcass characteristics of finishing beef steers
Authors: Heldt Jeff S , Davis Shane 
Published: July 2019

Abstract
The objective of this study was to determine if different sources and levels of supplemental copper (Cu), zinc (Zn), and manganese (Mn) affect growth performance, health and carcass characteristics of finishing beef steers. A large pen, commercial feedlot in OK utilized 1,707 crossbred steers (297 ± 5 kg) randomized across 8 pens/treatment. Supplemental trace mineral treatments consisted of: Control (CON) = CuSO4 plus Availa® Cu (10.6 ppm), ZnSO4 plus Availa Zn (37.8 ppm), and MnSO4 plus Availa Mn (25.5 ppm); Combination (COMBO) = basic Cu chloride (IntelliBond® C; 10 ppm), ZnSO4 plus Zn methionine (ZINPRO® 120; 90 ppm), and MnSO4 plus Mn hydroxychloride (IntelliBond M; 20 ppm); or Hydroxy (HYD) = basic Cu chloride (IntelliBond C; 10 ppm), Zn hydroxychloride (IntelliBond Z; 90 ppm), and MnSO4 plus Mn hydroxychloride (IntelliBond M; 20 ppm). Statistical analysis was performed using SAS with pen as the experimental unit and pre-planned orthogonal contrasts to separate means. Contrasts were: CON vs. COMBO and HYD (level) and COMBO vs. HYD (source). Supplemental trace mineral source or level did not affect final BW (P ≥ 0.34), ADG (P ≥ 0.36), DMI (P ≥ 0.36), feed efficiency (P ≥ 0.30), morbidity (P ≥ 0.28), total treatment days (P ≥ 0.43), or mortality (P ≥ 0.33) (Table 1). Additionally, trace mineral source or level did not affect HCW (P ≥ 0.78), DP (P ≥ 0.85), or backfat thickness (P ≥ 0.32) (Table 2). Increased level of trace mineral tended (P = 0.13) to increase marbling score. There was tendency for COMBO to have a larger loin muscle area (P = 0.14) and lower yield grade (P = 0.10) than HYD. Feeding a lower level of sulfates and amino acid complexes resulted in similar performance to higher trace mineral levels. Providing 100% Zn from Zn hydroxychloride resulted in an economic benefit while maintaining similar performance and carcass characteristics as feeding a combination of ZnSO4 and Zn methionine.
350 Effects of swab type on detection of PEDV from feed manufacturing surfaces
Authors: Stewart Savannah C , Niederwerder Megan C , Woodworth Jason C , Dritz Steve S , Jones Cassandra K 
Published: July 2019

Abstract
As pathogens become more prevalent within the swine industry, the demand for methods to monitor and detect them has increased. Using environmental swabbing to detect pathogens in the feed supply chain may allow for a greater likelihood of detection than product sampling. The objective of this experiment was to identify the swab that detects porcine epidemic diarrhea virus (PEDV) from feed manufacturing surfaces at the dose most similar to that included in an ingredient. The experiment was arranged as a 2×2×4 factorial with two doses of PEDV (low: 10^3 or high: 10^5 TCID50), two surface types (stainless steel or polyethylene tote), and four swab types (1: sponge-tip swab packaged with neutralizing buffer; 2: Dacron-tip swab packaged in Dey-Engley neutralizing broth; 3: dry dust-mop; or 4: cotton gauze soaked in phosphate-buffered saline). Soybean meal (SBM) was inoculated with levels of PEDV and placed onto stainless steel for 15 min, then the polyethylene tote. The SBM was removed, surfaces were swabbed with one of four types. Samples of inoculum, SBM, and swabs were analyzed for PEDV via qRT-PCR with three replicates per treatment. Data were analyzed via the GLIMMIX procedure of SAS. There was a dose×surface type×swab type interaction (P < 0.0001; SEM=0.967; Table 1). In stock virus and SBM, increased dose resulted in lower (P < 0.05) detectable PEDV. However, no virus was detected in sponge-tip swabs used on stainless steel surfaces. Dacron-tip and cotton gauze swabs yielded the most detectable PEDV from surfaces, but was still 1.2 to 5.1 Cycle threshold (Ct) higher than SBM. Detectable PEDV in SBM was approximately 11 Ct higher than in stock virus. In summary, environmental swabbing can easily detect PEDV, but at higher Ct than product samples. Of the swabs tested, Dacron-tip and cotton gauze are superior for PEDV environmental analysis than dry mops. Sponge-tip swabs should not be used for environmental monitoring of PEDV.
354 The effect of OvuGel® administered to weaned sows at proestrus or estrus on reproductive performance
Authors: Minton Amanda M , Shull Caleb M , Schwab Clint R , Buysse Steven G , Johnston Mike E , Webel Stephen K , Arend Lidia S , Knox Robert V 
Published: July 2019

Abstract
The use of single-fixed time insemination enhances leverage of genetically superior boars. It is prudent to elucidate the appropriate strategy for using OvuGel® by season and parity given the potential economic return. It was hypothesized that OvuGel® could be used to mitigate seasonal anestrus, in particular for parity 1 sows. The objective was to evaluate reproductive performance of weaned sows administered OvuGel® (OG; n = 1342) that expressed proestrus or estrus on d 4 post-weaning, followed by a single post-cervical artificial insemination (PCAI) 24 h later. This protocol was evaluated as an alternative to treating all weaned sows regardless of estrous behavior. The Control (CO; n = 1530) sows were inseminated via PCAI after they were identified in estrus and again 24 h later if still expressing estrus. Sows were inseminated from May through November 2017. Prior to weaning, within each replicate, sows were equally assigned to OG or CO treatment by parity, genetic line, litter size, nurse sow status and health treatment status. Farrowing rate for OG was not different than CO (86.2 ± 1.2 vs. 86.6 ± 1.2%; P > 0.10, respectively). There was no effect on total born between OG and CO (13.2 ± 0.1 vs. 13.3 ± 0.1; P > 0.10, respectively). However, there was a parity × treatment interaction. Parity 1 OG (86.6 ± 3.0%) sows had greater farrowing rates than CO (74.5 ± 3.0%) sows (P < 0.01), whereas there were no differences (P > 0.05) between treatments for the other parities. These data indicate that producers can administer OvuGel® to sows in proestrus or estrus on d 4 post-weaning, followed by a single insemination 24 h later, and achieve similar performance as sows inseminated multiple times upon detection of estrus.
353 Optimal dietary lysine for growth development of gilts until first parity
Authors: Supakorn China , Lents Clay A , Martinez Xochitl , Vallet Jeff , Dean Boyd R , DeDecker Ashley , Stalder Kenneth J 
Published: July 2019

Abstract
Unacceptable sow longevity in modern production systems has placed nutritional programs for developing gilts into question. Nutritional programs are well defined for terminal pigs, but not for developing gilts. To achieve breeding targets and allow gilts to meet their maternal genetic potential for lifetime productivity, the optimum lysine requirement for developing gilts should be well understood. The National Pork Board Animal Science Committee commissioned trials to determine the optimum lysine levels for developing gilts in U.S. pig production systems. An experiment was conducted to challenge proposed dietary lysine concentration for optimum growth rate during gilt development. A total of 2,960 growing crossbred gilts were fed three grower-finisher diets, formulated to provide low lysine (0.68 and 0.52% SID lysine), medium lysine (0.79 and 0.60% SID lysine), and high lysine (0.90 and 0.68% SID lysine) levels and fed to developing gilts starting from 142 to 200 d of age. Gilts fed the high lysine dietary treatment had significantly increased body weight (BW), flank-to-flank length, backfat thickness (BF), loin depth (LD), average daily gain, and fat-to-lean ratio (P < 0.05). Moreover, gilts fed the high lysine treatment during development phase had greater BW (202.4 ± 1.2 kg), BF (14.8 ± 0.2 mm), and LD (5.4 ± 0.5 cm) at first farrowing and BF (15.1 ± 0.2 mm) at weaning when compared with gilts fed the medium and low lysine treatments (P < 0.05). Litter birth weight (17.4 ± 0.3 kg), number weaned (9.0 ± 0.2), and litter weaning weight (46.6 ± 0.9 kg) from the gilts fed the high lysine sow treatment were greater when compared with performance from gilts fed the medium and low treatments (P < 0.05). Gilt development diets containing 2.79 g and 2.08 g SID lysine per MCal ME (high lysine grower and finisher diets, respectively) allowed gilts to achieve good growth and first parity reproductive performance.
358 Comparison of analytical methods for determination of nutrient concentration in beef cow colostrum and milk
Authors: Rathert Abigail R , Stephenson Emma L , Kenny Ann L , Meyer Allison M 
Published: July 2019

Abstract
Our primary objective was to determine the validity of Dairy Herd Improvement Association (DHIA) nutrient composition analysis for beef cow colostrum and milk by comparing DHIA with wet chemistry assays for protein, lactose, and milk urea nitrogen (MUN). A secondary objective was to analyze the change in milk nutrient composition over time in beef cattle. Colostrum and milk were collected from multiparous, fall-calving, Sim-Angus cows [n = 43; BW: 649 ± 80 (SD) kg; BCS: 5.3 ± 0.5; age: 4.3 ± 1.2 yr]. Colostrum was collected from a single rear-quarter pre-suckling, and milk was collected during 4-h milk yields at 35 and 60 d post-calving. Sub-samples were sent for DHIA analysis and frozen for colorimetric assays utilizing commercial kits run on a UV-visible light microplate reader. Analytical methods were compared using a general linear model, and nutrient composition across day was analyzed using a mixed model with day as a repeated effect. When comparing wet chemistry and DHIA colostrum analysis, r2 were 0.142 (P = 0.06), 0.348 (P = 0.002), and 0.0004 (P = 0.91) for protein, lactose, and MUN, respectively. Colostrum protein analyses had a negative relationship, whereas lactose was positive. For 35 d milk, r2 were 0.217 (P = 0.002), 0.128 (P = 0.02), and 0.248 (P = 0.001) for protein, lactose, and MUN, respectively. Comparing 60 d milk, r2 (P < 0.001) were 0.706, 0.314, and 0.381 for protein, lactose, and MUN, respectfully. All milk relationships were positive. There was an effect of day on nutrient concentrations, where colostrum contained greater (P < 0.001) protein than 35 and 60 d milk, whereas lactose and MUN were greatest (P < 0.001) in 35 d milk, and lowest (P < 0.001) in colostrum. These data indicate that beef cow colostrum and milk require wet chemistry analysis for improved accuracy.
360 Effect of housing beef cows on dry lots vs pasture on cow performance and reproduction
Authors: Cooney Ashley E , Neira Lucas T , Chapple Wesley P , Meteer William T , McCann Joshua C , Shike Daniel W 
Published: July 2019

Abstract
The objectives were to compare the effects of housing cows in dry lots or pasture on cow performance and reproduction. Simmental × Angus (n = 108; 87 ± 11.5 d postpartum), lactating, spring-calving cows were stratified by age, BW, BCS, calving date, and sex of the calves and allotted to six groups. Groups were randomly assigned to 1 of 2 treatments: dry lot (DL) or pasture (PAST). The cows in dry lot were limit-fed a ration consisting of corn silage, dried distillers grains, corn stalks, corn, and soybean hulls to meet their protein and energy requirements. Cows on pasture were rotationally grazed and with free-choice mineral. On d 0, cows were synchronized and artificially inseminated (AI). On d 0, 33, 51, and 87, BW and BCS were measured. Cow AI and overall pregnancy were determined on d 35 and 88, respectively. On d 52, milk production was determined using the weigh-suckle-weigh technique. Body weight, BCS, and milk yield were analyzed using the MIXED procedure of SAS. Reproductive data were analyzed using the GLIMMIX procedure of SAS. On d 0 and 33, BW did not differ (P ≥ 0.38) between cows on in DL or on PAST. On d 51, DL cows tended (P = 0.07) to have greater BW than PAST cows. On d 87, DL cows had greater BW (P = 0.05) than PAST cows. The BCS, milk yield, and reproductive rates did not differ (P ≥ 0.12) between treatments. Housing cows in dry lots compared to pasture increased BW, but did not affect BCS, milk production, and reproduction.