bluuebunny/crossref_metadata_2025_split huggingface dataset:
Unravelling the Molecular Dialogue of Beneficial Microbe−Plant Interactions
Authors: Srivastava Ashish K. , Singh Reema D. , Pandey Girdhar K. , Mukherjee Prasun K. , Foyer Christine H. 
Published: November 2024

ABSTRACTPlants are an intrinsic part of the soil community, which is comprised of a diverse range of organisms that interact in the rhizosphere through continuous molecular communications. The molecular dialogue within the plant microbiome involves a complex repertoire of primary and secondary metabolites that interact within different liquid matrices and biofilms. Communication functions are likely to involve membrane‐less organelles formed by liquid−liquid phase separation of proteins and natural deep eutectic solvents that play a role as alternative media to water. We discuss the chemistry of inter‐organism communication and signalling within the biosphere that allows plants to discriminate between harmful, benign and beneficial microorganisms. We summarize current information concerning the chemical repertoire that underpins plant−microbe communication and host‐range specificity. We highlight how the regulated production, perception and processing of reactive oxygen species (ROS) is used in the communication between plants and microbes and within the communities that shape the soil microbiome.
GmSTOP1‐3 Increases Soybean Manganese Accumulation Under Phosphorus Deficiency by Regulating GmMATE2/13 and GmZIP6/GmIREG3
Authors: Liu Guoxuan , Chen Qianqian , Li Dongqian , Mai Huafu , Zhou Yuming , Lin Maoxin , Feng Xiaonan , Lin Xiaoying , Lu Xing , Chen Kang , Tian Jiang , Liang Cuiyue 
Published: November 2024

ABSTRACTMineral nutrient deficiencies and metal ion toxicities coexist on acid soils. Phosphorus (P) deficiency in plants is generally accompanied with significant levels of leaf manganese (Mn) accumulation. However, the molecular regulatory mechanisms underpinning remain unclear. The present study found that P‐deficient soybean plants accumulated more Mn compared to P‐sufficient ones on acid soils in both field and greenhouse experiments. Meanwhile, both P deficiency and Mn toxicity enhanced the expression of GmSTOP1‐3. Over‐expressing GmSTOP1‐3 enhanced Mn accumulation in transgenic soybean hairy roots, but RNA‐interference did not show obvious differences. Moreover, transgenic soybeans with GmSTOP1‐3‐overexpression showed enhanced root citrate exudation and augmented Mn accumulation. RNA‐sequence identified four downstream genes of GmSTOP1‐3, including multidrug and toxic compound extrusion (GmMATE2/13) and metal transporter genes (GmZIP6/GmIREG3), which encode plasma membrane proteins. GmSTOP1‐3 activated the transcription of these four genes by directly binding to their promoter regions. In addition, both GmZIP6 and GmIREG3 functioned in Mn uptake as manifested by the higher Mn concentration and decreased growth of soybean hairy roots with their overexpression. Taken together, it is suggested that upregulation of GmSTOP1‐3 by low P stress on acid soils activates transcripts of GmMATE2/13 and GmZIP6/GmIREG3, which consequently result in enhanced Mn accumulation in soybean.
Hyperspectral Leaf Reflectance Detects Interactive Genetic and Environmental Effects on Tree Phenotypes, Enabling Large‐Scale Monitoring and Restoration Planning Under Climate Change
Authors: Corbin Jaclyn P. M. , Best Rebecca J. , Garthwaite Iris J. , Cooper Hillary F. , Doughty Christopher E. , Gehring Catherine A. , Hultine Kevin R. , Allan Gerard J. , Whitham Thomas G. 
Published: November 2024

ABSTRACTPlants respond to rapid environmental change in ways that depend on both their genetic identity and their phenotypic plasticity, impacting their survival as well as associated ecosystems. However, genetic and environmental effects on phenotype are difficult to quantify across large spatial scales and through time. Leaf hyperspectral reflectance offers a potentially robust approach to map these effects from local to landscape levels. Using a handheld field spectrometer, we analyzed leaf‐level hyperspectral reflectance of the foundation tree species Populus fremontii in wild populations and in three 6‐year‐old experimental common gardens spanning a steep climatic gradient. First, we show that genetic variation among populations and among clonal genotypes is detectable with leaf spectra, using both multivariate and univariate approaches. Spectra predicted population identity with 100% accuracy among trees in the wild, 87%–98% accuracy within a common garden, and 86% accuracy across different environments. Multiple spectral indices of plant health had significant heritability, with genotype accounting for 10%–23% of spectral variation within populations and 14%–48% of the variation across all populations. Second, we found gene by environment interactions leading to population‐specific shifts in the spectral phenotype across common garden environments. Spectral indices indicate that genetically divergent populations made unique adjustments to their chlorophyll and water content in response to the same environmental stresses, so that detecting genetic identity is critical to predicting tree response to change. Third, spectral indicators of greenness and photosynthetic efficiency decreased when populations were transferred to growing environments with higher mean annual maximum temperatures relative to home conditions. This result suggests altered physiological strategies further from the conditions to which plants are locally adapted. Transfers to cooler environments had fewer negative effects, demonstrating that plant spectra show directionality in plant performance adjustments. Thus, leaf reflectance data can detect both local adaptation and plastic shifts in plant physiology, informing strategic restoration and conservation decisions by enabling high resolution tracking of genetic and phenotypic changes in response to climate change.
Factors Influencing Professional Help‐Seeking Intentions for Sexual and Gender‐Based Violence Among Female University Students in Eswatini
Authors: Jung Mi Sook , Dlamini Nondumiso Satiso 
Published: November 2024

ABSTRACTObjectivesTo investigate the prevalence of sexual and gender‐based violence (SGBV) among female college students, identify factors influencing intention to seek professional help among SGBV survivors, and compare help‐seeking determinants among survivors based on their prior utilization of healthcare services.DesignA cross‐sectional online survey was employed among female students aged 18–24 years from two universities in Eswatini.Measurements and SampleA total of 271 female college students were recruited to assess SGBV experience, attitudes, social norms, perceived control, help‐seeking intention, and other factors not originally included in the theory of planned behavior. Data analyses utilized descriptive statistics, comparative analyses, and multiple regression methods.ResultsAmong the participants, 73.1% reported experiencing one or more forms of SGBV, while only 43.4% sought professional help through health services. Help‐seeking intentions of survivors were significantly associated with positive attitudes, subjective norms, and marital status, collectively explaining 66% of the variance. In subgroup analyses, the subjective norm and attitude emerged as the most robust predictors of help‐seeking intentions.ConclusionThe results revealed a high prevalence of SGBV and a concerning trend of low help‐seeking behavior, with attitudes and subjective norms emerging as the most influential factors shaping intention. These results provide valuable insights for designing theory‐driven and culturally sensitive public health nursing interventions and educational programs aimed at effectively supporting SGBV survivors.
Developing a Scale to Measure Public Health Nurses’ Advocacy Practices in Group and Community Settings in Japan
Authors: Hatono Yoko , Suzuki Hiroko , Shimazu Taeko 
Published: November 2024

ABSTRACTObjectiveThis study aimed to develop a scale to assess public health nurses’ (PHNs’) advocacy practices in groups and communities in Japan and determine its reliability and validity.Design and SampleThis study is a cross‐sectional investigation. The sample comprised PHNs across various municipalities in Japan.MeasurementsAnonymous self‐administered questionnaires were conducted, including the attributes of PHNs, a preliminary scale, and external criteria. Reliability was assessed by calculating the alpha coefficient. To ascertain construct validity, exploratory and confirmatory factor analyses were conducted.ResultsA total of 432 questionnaires were analyzed. The scale comprised 27 items divided into five factors: “Raising awareness of the challenging situation faced by disadvantaged individuals,” “Empowering disadvantaged individuals to improve their situation autonomously,” “Establishing a foundation in local governments and community groups of disadvantaged individuals for advocacy initiatives,” “Working toward policy changes,” and “Collaborating with stakeholders and organizations to enhance the effectiveness of activities.” The Cronbach's alpha coefficient for this scale was 0.945. Model fit indices included a comparative fit index of 0.907, a goodness‐of‐fit index of 0.840, an adjusted goodness‐of‐fit index of 0.806, and a root mean square approximation of 0.067.ConclusionsThe developed scale demonstrated satisfactory reliability and validity.
Macrophytes Used as Multifaceted Benefits Including Feeding, Bioremediation, and Symbiosis in Freshwater Aquaculture—A Review
Authors: Mandal Rathidra Nath , Bera Pujadebi 
Published: November 2024

ABSTRACTThe utility of macrophytes in freshwater aquaculture is diverse. We review the benefits of macrophytes in aquaculture into broadly three useful perspectives: (i) Feeding, (ii) Bioremediation, and (iii) Symbiosis. Macrophytes are used as direct food by herbivores for their potential nutrient values with a maximal protein content of 35% (D/W basis) and as ingredients for fish feed preparation with optimal 50% protein inclusion to reduce feed cost in commercial aquaculture. Macrophytes are used for wastewater treatments to eliminate contaminants, including As, Cu, Cd, Cr, Hg, and Pb, to 99.9%, and to reduce BOD (Biological Oxygen Demand) to improve water quality. Intake of remedied wastewater into aquaculture enhances water productivity through phytoplankton growth. An estimate is that 1.0 L of remedied wastewater, particularly sewage effluent adds an average of 0.05 g N & P and 0.99‐L freshwater into aquaculture to yield an average of 0.309 g fish. Macrophytes are integrated with fish in commercial aqua‐farming. Plants and fish are farmed together in aquaponics. They are used as substrates for some commercial fish species to lay eggs in farming. They are raised with ornamental fish for benefits in aquariums. Nevertheless, extensive studies are required to harness more benefits from macrophytes.
Psychometric Properties of the Danish International Trauma Questionnaire—Child and Adolescent Version (ITQ‐CA)
Authors: Sigurðardóttir Katla , Qambar Noor , Elklit Ask , Auning‐Hansen Mikkel A. , Nielsen Sabrina B. 
Published: November 2024

ABSTRACTWith the introduction of the ICD‐11 diagnostic manual, the need for developing and validating new assessment instruments has become urgent. The International Trauma Questionnaire‐Child and Adolescent version (ITQ‐CA) assesses posttraumatic stress disorder (PTSD) and complex PTSD (CPTSD) based on the ICD‐11 diagnostic definition. This study examines the psychometric properties of the Danish version of the ITQ‐CA in a non‐clinical sample of 226 adolescents. Hierarchical multiple regression analyses were performed to study the effects of gender, age, trauma exposure, family dysfunction, and SDQ variables on PTSD and Disturbances in Self‐Organization (DSO). Results showed that 16 participants (7%) met the ITQ‐CA criteria for CPTSD and 11 (5.7%) for PTSD. The full model of gender, age, family dysfunction, trauma exposure, and SDQ variables was found to predict PTSD and DSO. The present study supports the validity of the Danish version of the ITQ‐CA and represents an important step of establishing and applying validated tools for assessing PTSD and CPTSD in children and adolescents. The results also underscore the need for a broader trauma definition. Future research should examine the sensitivity of the ITQ‐CA.
The influencing factors of willingness to participate in crop rotation and fallow: A behavioural economics perspective
Authors: Wu Haohao , He Linbo , He Junhong 
Published: November 2024

AbstractCrop rotation and fallow practices are increasingly adopted to mitigate ecological resource pressures and accelerate the development of modern agriculture. These practices have significant implications for environmental protection, soil quality enhancement and the promotion of sustainable, high‐quality agricultural development. Despite their importance, empirical research on the factors influencing participation in crop rotation and fallow remains limited and comparative analyses of different compensation scenarios are scarce. This study, grounded in behavioural economics, utilizes data from the China Rural Revitalization Survey (CRRS) and employs propensity score matching (PSM) and binary logistic regression models to analyse farmers' behaviour regarding both induced and voluntary crop rotation and fallow. The research aims to identify the factors that influence farmers' decisions to engage in these practices. The findings indicate that, under a fixed subsidy standard, the participation rates for crop rotation and fallow were 14.5% and 5.4%, respectively. Among the farmers participating in crop rotation, 43.8% were induced, while 56.2% were voluntary. For fallow practices, 47.9% of the participants were induced and 52.1% were voluntary. In the context of induced crop rotation and fallow, ecological subsidies significantly increased farmers' willingness to participate (p‐value <.001). Conversely, in voluntary contexts, resource endowment had a significantly positive impact on crop rotation behaviour (p‐value <.1), whereas path dependence and policy awareness significantly constrained fallow behaviour (p‐value <.05). The impact of ecological subsidies on farmers' crop rotation and fallow behaviour varied significantly across different ecological regions, with other factors also exhibiting notable heterogeneity. The results of this research offer valuable guidance for the implementation of future crop rotation and fallow programmes. Additionally, they provide insights into the relationship between ecological subsidy systems and farmers' welfare in other underdeveloped areas, suggesting improvements and offering new perspectives for further research.
Utilization of IGN historical aerial photographs and Google earth for measuring changes in land use and evolution of termite lenticular mound abundance in paddy fields in Cambodia
Authors: Muon Ratha , Zaiss Rainer , Lao Chanrithy , Ann Vannak , Jouquet Pascal 
Published: November 2024

AbstractThis study was conducted to investigate termite mounds' dynamics in paddy fields in Cambodia. Historical aerial images collected in the 50s by the French Institut Géographique National (IGN) and recent Google Earth (GE) were analysed to study land use changes and mound distribution in 30 plots. A significant decrease in the surface covered by scrublands and forests was measured (from 37% in 1953 to less than 2% in 2021). We observed that most mounds seen in the field in 2021 could also be seen in IGN and GE images (88.6%), indicating that mounds have a long lifespan but also that they can be built in less than 70 years. Mound density was neither influenced by the topography nor by the restructuring of the paddy field boundaries during the Khmer Rouge regime. However, areas that were more recently converted into paddy fields had more mounds compared to areas that were already paddy fields in 1953 (2.92 vs. 1.53 mounds ha−1, respectively). Therefore, deforestation and other environmental changes have turned mounds into remnants of the forests that had almost completely disappeared. This highlights the importance of protecting these specific environments in a changing world facing a major crisis of biodiversity loss.
TaWRKY24 integrates the tryptophan metabolism pathways to participate in defense against Fusarium crown rot in wheat
Authors: Xu Xing , Yu Tai‐Fei , Wei Ji‐Tong , Ma Xiao‐Fei , Liu Yong‐Wei , Zhang Jin‐Peng , Zheng Lei , Hou Ze‐Hao , Chen Jun , Zhou Yong‐Bin , Chen Ming , Ma Jian , Jiang Yun‐Feng , Ji Hu‐Tai , Li Li‐Hui , Ma You‐Zhi , Zhang Zhi‐An , Xu Zhao‐Shi 
Published: November 2024

SUMMARYWheat growth process has been experiencing severe challenges arising from the adverse environment. Notably, the incidence of Fusarium crown rot (FCR), a severe soil‐borne disease caused by Fusarium pseudograminearum (Fp), has significantly intensified in various wheat‐growing regions, resulting in a decline in grain yield. However, the identification of wheat varieties and the exploration of effective gene resources resistant to FCR have not yet been accomplished. Here, we screened and identified the tryptophan metabolism pathway to participate in wheat resistance to FCR by correlation analysis between transcriptome and metabolome, and found that indole‐3‐acetaldehyde (IAAld) and melatonin, two key metabolites in the tryptophan metabolic pathway, were significantly accumulated in Fp‐induced wheat stem bases. Interestingly, exogenous application of these two metabolites could significantly enhance wheat resistance against Fp. Additionally, we observed that the activity of TaALDHase, a crucial enzyme responsible for catalyzing IAAld to produce indole‐3‐acetic acid (IAA), was inhibited. Conversely, the activity of TaMTase, a rate‐limiting involved in melatonin biosynthesis, was enhanced in the Fp‐induced wheat transcriptome. Further analysis showed that TaWRKY24 could regulate IAA and melatonin biosynthesis by inhibiting the expression of TaALDHase and enhancing the transcription of TaMTase, respectively. Silencing of TaALDHase could significantly increase wheat resistance to FCR. However, interference with TaWRKY24 or TaMTase could decrease wheat resistance to FCR. Collectively, our findings demonstrate the crucial role of the tryptophan metabolism pathway in conferring resistance against FCR in wheat, thereby expanding its repertoire of biological functions within the plant system.
Risk of introducing Zika virus in the Canadian cord blood supply: A risk analysis
Authors: Lewin Antoine , O'Brien Sheila F. , Seftel Matthew , Latour Catherine , Allan David , Chouinard Marie‐Claude , Fournier Diane , Renaud Christian 
Published: November 2024

AbstractBackgroundIn Canada, as many as 24% of mothers are deferred from cord blood (CB) donation due to risk factors for Zika virus (ZIKV). However, the ZIKV epidemic has waned considerably since 2016, and there has not been any report of ZIKV transmission by CB transplantation, which questions this policy. Thus, we performed an analysis of the risk of introducing ZIKV in the CB supply maintained by Héma‐Québec (HQ) and Canadian Blood Services (CBS).Study Design and MethodsThis simulation considered the following parameters: the risk of travel exposure in a high‐risk ZIKV country, the duration of travel, the daily risk of acquiring ZIKV in a high‐risk country, the probability of materno‐fetal ZIKV transmission, the probability of asymptomatic fetal viremia, and the probability of sexual transmission. A hundred million Monte Carlo simulations were run.ResultsIn the most‐likely scenario (probability of traveling to a high‐risk ZIKV country while pregnant = 0.178), the risk was estimated at 0.9 ZIKV‐positive donations per million donations (95% confidence interval [CI] = 0.4–1.6)—or one every 868 years at HQ and one every 453 years at CBS. In the pessimistic model (probability of traveling to a high‐risk ZIKV country while pregnant = 0.240), the risk was estimated at 1.2 ZIKV‐positive donations per million donations (95% CI = 0.6–2.1)—or one every 644 years at HQ and one every 340 years at CBS.DiscussionWe conclude that the risk of introducing ZIKV in the Canadian CB supply is too small to justify maintaining the current policy.
Why is the Roy–Borjas model unable to predict international migrant selection on education? Evidence from urban and rural Mexico
Authors: Leopold Stefan , Ruhose Jens , Wiederhold Simon 
Published: November 2024

AbstractThe Roy–Borjas model predicts that international migrants are less educated than nonmigrants because the returns to education are generally higher in developing (migrant‐sending) countries than in developed (migrant‐receiving) countries. However, empirical evidence often contradicts this prediction, even when comparing migrants from different regions of the same country. Specifically, whilst Mexicans migrating to the United States from urban areas are negatively selected based on education, those from rural areas are positively selected – despite similar returns in both regions. Leveraging this setting, we demonstrate that the inconsistency between theory and evidence can be resolved by assessing migrants' human capital using a two‐dimensional measure of occupational skills rather than educational attainment. Our findings caution against using positive migrant selection on education as evidence to reject the Roy–Borjas framework for explaining international migration.
Exploring the imperative of “relational documentary”: Process, power, and unruly collaboration in media production on Palestine
Authors: Bishara Amahl 
Published: November 2024

AbstractBecoming an anthropologist of Palestine during the War on Terror meant that the specter of violence and exclusion always loomed over my research; yet I had become an anthropologist out of a commitment to presence in and engagement with people from the Middle East. I investigate the ways in which Faye Ginsburg's approach to studying polarizing issues, her analysis of Indigenous media, and her commitment to ethical collaboration has shaped my written and video work. In the first part of the paper, I discuss how reflexivity and positionality propelled me forward in an ethnographic study of journalistic production. In the second half, I reflect on collaborative film projects that illuminated themes of risk and positionality that are at the heart of politics and media production in Palestine. The model of “relational documentary” analyzed by Ginsburg that considers ethics and politics both on and off screen informs my approach to the obligations of long‐running partnerships. Just as much, Ginsburg's commitment to play and creativity allowed me to imagine unexpected explorations of mobility, voice, and place to ask what we can do, working across lines of difference, to be able to hear each other and make space for each other to be heard.
Outcomes and Perioperative Complications of Surgical Resection on Canine Carotid Paragangliomas (21 Cases): A Veterinary Society of Surgical Oncology Study
Authors: Paskoff Kathryn Marie , Matz Brad , Fournier Quentin , Aisa Josep , Oblak Michelle L. , Bray Jonathan , Linden Daniel 
Published: November 2024

ABSTRACTCarotid body paragangliomas represent an uncommon neoplasm in dogs. The objective of this study was to report outcomes and complications associated with surgical excision of carotid body paragangliomas in 21 dogs. Cases were recruited retrospectively via medical record review from 9 veterinary speciality centres. The perioperative complication rate was 52% (11/21). Complications encountered in this cohort related to removal of carotid body tumour included airway obstruction, aspiration pneumonia, megaesophagus, unilateral laryngeal paralysis, coughing and Horner's syndrome. The overall perioperative mortality rate was 4.7% (1/21 dogs) and median survival time was 554 days for the six patients with known dates of death. One‐ and two‐year survival rates were 61% and 42%, respectively. This is the largest collection of carotid body paraganglioma cases reported in veterinary literature. Based on these results, surgical resection of carotid body paragangliomas was associated with low perioperative mortality and long survival times.