bluuebunny/crossref_metadata_2025_split huggingface dataset:
Microwave-assisted preparation of yeast cells for ultrastructural analysis by electron microscopy
Authors: Mayer Moritz , Schug Christina , Geimer Stefan , Klecker Till , Westermann Benedikt 
Published: November 2024

Abstract
Budding yeast Saccharomyces cerevisiae is widely used as a model organism to study the biogenesis and architecture of organellar membranes, which can be visualized by transmission electron microscopy (TEM). Preparation of yeast cells for TEM can be quite challenging and time-consuming. Here, we describe an optimized protocol for conventional fixation of yeast cells with potassium permanganate combined with cell wall permeabilization with sodium metaperiodate and embedding in Epon. We have replaced time-consuming incubation steps by short treatments with microwaves and developed a microwave-assisted permanganate fixation and Epon embedding protocol that reduces the time required for sample preparation to one working day. We expect that these protocols will be useful for routine analysis of membrane ultrastructure in yeast.
Structure and electrical transport properties of cation-deficient derivatives of layered neodymium–barium ferrocuprocobaltite
Authors: Chizhova Ekaterina A. , Marozau Maksim V. , Shevchenko Svetlana V. , Klyndyuk Andrei I. , Zhuravleva Yana Yu. , Kononovich Vladimir M. 
Published: November 2024

Layered double perovskites (LDP) based on rare earth elements, barium, and 3d-metals with high electrical conductivity and electrocatalytic activity in the oxygen reduction reaction are promising cathode materials for medium-temperature solid oxide fuel cells based on proton- or oxygen-conducting solid electrolytes. For the improvement of the functional characteristics of LDP, various strategies are used: a) creating composites based on LDP, b) the partial substitution of cations, and c) the creation of a deficiency of cations in various positions in the LDP structure. The advantage of the latter strategy is that it does not require complicating the chemical and, as a rule, phase composition of the LDP. The purpose of this study was the investigation of the effect of neodymium and barium deficiency on the structural and electrical transport characteristics of NdBaFeCo0.5Cu0.5O6–d LDP.
The samples were obtained by the ceramic method and characterized using X-ray phase analysis, IR absorption spectroscopy, iodometry, electron microscopy, thermal analysis, as well as electrical conductivity and thermo-EMF measurements methods.
Creation of up to 10 mol. % of vacancies in neodymium or barium sublattices had little effect on the values of the oxygen nonstoichiometry index (d) and unit cell parameters of NdBaFeCo0.5Cu0.5O6–d derivatives. owever, it led to an increase in the crystallite size (determined by the Scherrer, Williamson–Hall and size–strain methods) and the thermal stability of these phases. The values of electrical conductivity and the Seebeck coefficient of ceramics, in general, increased, and the activation energies of the electrical transfer process decreased when a deficiency of neodymium or barium was created in its structure. In the temperature range 300–700 K, the weighted mobility of charge carriers (“holes”) varied within 0.04–0.8cm2/(V·s) and increased with increasing temperature, which is typical for the polaron conduction mechanism, and their concentration varied in the range (0.1–3)·1020 cm–3, increased exponentially with increasing temperature and, in general, when a deficiency of neodymium or barium in the NdBaFeCo0.5Cu0.5O6–d structure was created
A molecular dynamics simulation of the release of desloratadine from alloys containing polyvinylpyrrolidone
Authors: Polkovnikova Yulia A. , Belal Mohamed , Slivkin Alexey I. 
Published: November 2024

Computer modeling is currently a promising technique used in pharmaceutical technologies to develop drug compositions. Molecular dynamics has provided space and time resolutions unavailable during experiments and thus has greatly extended the capabilities of chemistry and some other areas. Molecular dynamics stimulations are very important for the development of solid drug dispersions. The purpose of this study is to simulate the molecular dynamics of the release of desloratadine from alloys containing polyvinylpyrrolidone-10000 into the dissolution medium.
The release of desloratadine from alloys containing polyvinylpyrrolidone-10000 was simulated by the method of molecular dynamics (Gromacs 2023 program, Amber 99 force field). The study involved calculating van der Waals energies of interaction between desloratadine and PVP and desloratadine and water and the proportion of desloratadine molecules that lost their bonds with PVP. The desloratadine molecule was considered released into water provided that it did not bind either to the polymer or water.
It was found that the degree of desloratadine release from PVP into the aqueous medium was the highest at a ratio of 1:1 (24.56±2.08%), and the lowest at ratios of 1:2 and 1:5 (8.27±1.79 and 8.65±0.98%, respectively). At a ratio of 1:1, the average energy of interaction between desloratadine with PVP per one molecule of desloratadine was the highest (–36.13±0.62 kJ/mol) when the energy of interaction between desloratadine and water was low (–52.03±0.82 kJ/mol), which indicates that desloratadine involvement in the solvation and desorption processes was the highest at this ratio. The average energy of interaction between desloratadine and the polymer was the lowest at a ratio of 1:5 (–52.03±0.82 kJ/mol) when the energy of interaction between desloratadine and water was –44.45±1.60 kJ/mol. This fact indicates a low intensity of the esorptionand solvation processes at this ratio
Improving breast-cancer diagnostics: A study using AI-ML with computational prognostics and feature extraction
Authors: Raju V Rama , Devi G Naga Rama 
Published: November 2024

: Though and even though advancements are high during last two decades, yet ‘breast—cancer’ continues to be a sizable causal transience amongst females (womankind) globally. Whilst mammography has distinctly lowered death rates, detection and categorization and accuracy of breast sizes (forms in quantities) in mammograms remains argue. Thus we present a new method of detection and discovery of key region-of-interest(ROI) plus its labeling as natural usual ‘0’, benign ‘1’, or cancerous ‘2’ sizes. : For sorting of identified ‘ROIs’ as usual, benign or malicious exactly. : We use the removal of temporally sequential digital mammograms, merged via machine-learning (ML). We estimated feature-selection algorithmic techniques (ATs) over the novel database (data—set) comprises images~circa352 as of 88 diseased exactly noted by sites-of-mass. 98 feature extractions were done and classified by 8 dissimilar feature-selection ATs to detect the best discriminative features. We then computed 10classifiers via first in first out (FIFO) diseased plus a k-fold cross validation. In our computation, we observed the artificial neural nets (ANNs) appeared as the highest classifier with 99.5%accuracy, 0.98AUC (for label ‘0’), 0.92AUC (for label ‘1’, plus 0.94AUC (for class ‘2’). : Our findings showed significant progress in divergence (disparity) through existing advanced high-tech algorithms and techniques, which feature worth of using temporally successive mammographs in amalgamation, with advanced computational intelligence ML, aimed at the exact sorting of identified ‘ROIs’ as usual, benign or malicious. : Clinical significancy/ when clinically applied, this study improves the clinical prognosis precision of breast-cancer possibly steering to reliable diseased outcomes plus added personalized therapeutic attempts.
Use of ambulatory blood pressure monitoring in hypertensive patient to monitor blood pressure control
Authors: Kurumbang Janer , Dahal Tshetiz 
Published: November 2024

To use ambulatory blood pressure monitoring in hypertensive patient for better control of their blood pressure. This was a single centre, observational study that included hypertensive patients who visited the out-patient department of tertiary care hospital Nepal. The study was carried out from June, 2021 to June, 2023. All the patients were assessed through ambulatory blood pressure monitoring machine for 24 hours. Total 259 patient were studied with ambulatory 24 hours blood pressure monitoring. Average age was 44.82±13.67 years. 30 to 50 age group was the maximum user of ABPM (41%) 20 patient in this study were ≥65years of age. 58 % patients were males and 42% were female. All the patient undergoing ABPM were hypertensive. Mean of 24 hours systolic BP was 129.69 ± 12.86 mmHg and diastolic BP was 81.91±9.2 mmHg, awake systolic BP was 134.5 ± 12.5 mmHg and diastolic BP was 86.32±9.44 mmHg, asleep systolic BP was 121± 15.1 mmHg and diastolic BP was 74.46±10.38 mmHg. Only 28 (10.8%) patient of 259 had normal mean daytime blood pressure while 21 patient 8.1% had elevated BP and 210 patient 81.1% had hypertension as per AHA/ACC 2017 classification. Of total hypertensive 210 patient 84% had grade 2 hypertension and 16% had grade 1 hypertension requiring intensification of medication to control blood pressure in majority of the patient. Most of the patient treated with hypertension had inadequate control as seen on ambulatory blood pressure monitoring. ABPM should be utilized in every hypertensive patient to monitor the adequate control of BP.
Examining the relationship between medical doctors\' lifestyle behaviors and the incidence of lifestyle-related diseases: An observational cohort study
Authors: Godse Ajay M , Gosavi Nayana B , Karande Bhagyashri L , Nawathe Vaidehi A 
Published: November 2024

Evaluating the presence of health-related lifestyle factors among physicians is crucial, as adopting healthy behaviors can significantly reduce mortality and morbidity, and positively impact patient counselling. Despite the expectation for physicians to advocate healthy behaviors to their patients, they themselves often fall short of meeting these standards of healthy lifestyle. Research indicates that risky behaviors such as alcohol consumption, tobacco use, and unhealthy eating habits tend to co-occur among physicians. This study aimed to assess the lifestyle behaviors of medical professionals at tertiary care center in order educate doctors about health-promoting behaviors and practices.This prospective single center study recruited 74 doctors who were asked to fill questionnaire through google form eliciting existing comorbid conditions and habits, followed by physical assessment which comprised Blood pressure, height, weight, BMI, pulse, oxygen saturation (SpO2), neck & hip circumference, body composition & fat analysis.No significant association was observed between physical activity and visceral fat (p-value = 0.38), nor between physical activity and body fat composition (p-value = 0.57). However significant association between visceral fat levels and BMI with respect vegetarian and non-vegetarian dietary patterns were observed, highlighting the importance of diet for optimal health.Study highlights necessity for a carefully devised strategy for medical proffesionals to have healthy dietary habits and regular exercise into daily routines, to not only have professional well-being but also as advocates for promoting overall physical and psychological health within society.
Assessment of the knowledge about blood donation among medical students: Before and after implementing a designed teaching session about blood donation
Authors: Mohan Roshan , Gotekar Yashodhara Rajesh 
Published: November 2024

: Human blood is most important body fluid in saving lives .Evidence indicates that there is a shortage of blood and blood products in many countries. This study aimed at assessing knowledge towards blood donation and its associated factors. With focus on encouraging voluntary blood donation. That’s why stress should be given on voluntary, non - remunerated blood donation. This study aims to determine knowledge about voluntary blood donation, and the objective was to educate undergraduate students about voluntary blood donation. Undergraduates who expressed their consent for participation were enrolled in the study. A pre‑ tested, structured questionnaire was used as a study tool. Baseline information were collected, a brief interactive awareness session, addressing voluntary blood donation was organized for the participants and their willingness to donate blood was again noted at the conclusion of the session. A total of five hundred undergraduate medical students enrolled , out of which 270 (54%) were male and 230 (46%) female. The overall knowledge of voluntary blood donation increased from 67% to 89.90% after educational intervention. Undergraduate medical students had good baseline knowledge about blood donation and this further improved after blood donation educational intervention.
Na pograniczu ideologii, komercji i wychowania. O współczesnej rosyjskiej animacji telewizyjnej dla dzieci
Authors: Śmiech Jaśmina 
Published: November 2024

Postrzeganie sztuki animacji jako narzędzia krzewienia określonego światopoglądu i hierarchii wartości ma w Rosji długą tradycję. Współcześnie film animowany jest traktowany przez rosyjskie instytucje państwowe i medialne jako podstawowy rodzaj sztuki filmowej przeznaczonej dla dzieci, na którym ciążą zadania wychowawcze i edukacyjne. Celem artykułu jest wykazanie powiązań pomiędzy całokształtem komunikacji werbalnej obecnej we współczesnych rosyjskich telewizyjnych filmach animowanych dla dzieci a aktualnymi wartościami społecznymi, instytucjami wpływającymi na produkcję i dystrybucję animacji dla dzieci oraz praktykami społecznymi kształtującymi preferencje wobec określonych konwencji filmowych. Analiza ma charakter mediolingwistyczny i obejmuje takie zagadnienia językowe jak: złożoność i nacechowanie stylistyczne tekstu, staranność wypowiedzi i kultura słowa, obecność elementów specyficznych kulturowo, aluzyjność oraz komizm. Prowadzi ona do wniosków, że preferowana jest prostota, neutralność stylistyczna oraz jednoznaczność przekazu, której nie powinien osłabiać komizm językowy. Pożądana jest obecność elementów specyficznych kulturowo, choć ich stopień zespolenia z tekstem bywa zróżnicowany.
PERBANDINGAN BIAYA DAN WAKTU ANTARA METODE KONVENSIONAL DENGAN METODE MODULAR MENGGUNAKAN MICROSOFT PROJECT
Authors: Huwaida Nabila Yusriyya , Handayani Fajar Sri , Rifai Muji 
Published: November 2024

<p><em>Indonesia's construction sector is experiencing rapid growth, this proven by numerous development projects, including a sub-district building construction project, aiming to optimize community services. </em><em>A project must have three aspects, namely cost, quality and time. Choosing an implementation method is very important </em><em>so that a project can achieve these three goals.</em><em> </em><em>The research method used a quantitative descriptive approach with the aim of comparing cost and time aspects between conventional methods and modular methods. The conventional approach involves working on site using conventional building materials, while the modular approach involves the use of pre-fabricated modules. In analyzing the costs and time of construction of the Grabag Sub-district office building, Purworejo Regency, Microsoft project application was used to calculate the duration of the work. The cost analysis show that building structure work using the conventional method costs Rp.3,086,929,413.11, while the modular method costs Rp.3,646,018,620.52. So the difference in the cost of construction work is Rp.559,089,207.42 or 18.11% higher for work using the modular method. The time analysis obtained building structure work using conventional methods require 150 days while the modular method is 87 days or time efficiency of 63 days or 42% lower for work with modular methods. The results of the Time Cost Trade Off (TCTO) calculation showed that the total project cost for conventional construction was Rp.2,781,017,489 while the cost for modular construction was Rp.3,117,530,328. So a difference of Rp.336,512,839 is higher for modular construction. Therefore, conventional construction is the most economical option for the sub-district building.</em></p>
Rancangan kemasan pelindung apel selama tranportasi berbahan dasar eceng gondok dan sabut kelapa
Authors: Widhiantari Ida Ayu , Amuddin Amuddin , Ridho Rosyid , Khalil Fakhrul Irfan , Puspitasari Isnaini 
Published: November 2024

Packaging during the transportation process greatly influences the damage to horticultural products. Harvest apples are usually packed in wooden boxes or bamboo baskets and stacked until they reach maximum capacity. This makes the fruit easily damaged by bruising due to high piles. Based on these conditions, it is necessary to handle horticultural products in the form of apples to reduce damage to the fruit during transportation to maintain the quality of the apples until they reach consumers and extend the shelf life of the fruit. This research aims to design protective packaging for apples from a combination of water hyacinth and coconut fiber. The experimental method was carried out in this research with 2 factors consisting of 3 levels; factor one the combination of packaging materials, and factor two was the pressure of the packaging printer. Nine experimental units were obtained from the combination of the two treatments, namely K1T1 (E 90% + S 10%) with a pressing pressure of 400 psi, K1T1 (E 90% + S 10%) with a pressing pressure of 700 psi, K1T1 (E 90% + S 10% ) with a pressing pressure of 1000 psi, K2T1 (E 60% + S 40%) with a pressing pressure of 400 psi, K2T2 (E 60% + S 40%) with a pressing pressure of 700 psi, K2T3 (E 60% + S 40%) with pressing pressure 1000 psi, K3T1 (E 30% + S 70%) with pressing pressure 400 psi, K3T2 (E 30% + S 70%) with pressing pressure 700 psi, K3T3 (E 30% + S 70%) with pressing pressure 1000 psi. The results showed that the combination treatment of K2T2 with a pressing pressure of 700 psi provided the best protective quality for apples with the lowest percentage of mechanical damage and reduction in apple mass, at 5.556%, 2.55%, respectively. The springiness and texture values for apples were the highest with values respectively 3.17 mm, 16.7335N. Meanwhile, the highest packaging compressive strength value was obtained in the K3T3 treatment with a value of 26.164N.
Association between endoscopic and radiological activity of inflammatory bowel disease versus dietary inflammatory index and body adiposity
Authors: de Oliveira Rubia , Vasques Ana Carolina , Ribeiro Stefhani , Shivappa Nitin , Hebert James , Nagasako Cristiane 
Published: November 2024

Abstract

Background
Inflammatory Bowel Disease (IBD) has genetic and environmental factors. However, the relationship between activity of IBD, adiposity and dietary pattern has not yet been fully clarified.
Objective
Investigate the association between endoscopic and radiological activity of IBD, body adiposity and dietary inflammatory index (DII).
Method
An observational, cross-sectional study was carried out. Endoscopic activity was defined by: endoscopic Mayo score > 2, Crohn’s Disease Endoscopic Index of Severity (CDEIS) > 5, and/or presence of a deep ulcer in any intestinal segment. Body adiposity was estimated using the body mass index, waist circumference, and waist-hip ratio (WHR). The DII score was calculated from a validated quantitative food frequency questionnaire. According to the DII, the patients were divided into three groups: the first with the less pro-inflammatory diet and the third with a predominantly pro-inflammatory diet.
Results
62 patients were assessed, 58.06% in remission disease (RD) and 41.93% in activity (AD). Overweight/obesity were more frequent in the RD group (p = 0.12); as well as high WHR (p < 0.05) and central obesity (p < 0.01). A predominance of pro-inflammatory diet was observed, both in the AD and RD groups, since most patients were in group 3 in the evaluation of DII and DII adjusted for energy density (E-DII) (p = 0.77 and p = 0.18).
Conclusions
high frequency of a proinflammatory diet and high rates of overweight and obesity were observed in the IBD population, with patients in remission showing higher WHR and central obesity. DII and EDII, were not associated with endoscopic and radiological disease activity in patients with IBD.
Vulnerable and vital: Intermittent estuaries deserve global attention
Authors: Khojasteh Danial , Rao Shivanesh , McSweeney Sarah , Ibaceta Raimundo , Nicholls Robert , French Jon , Glamore William , Largier John , Adams Janine , Hughes Michael , Barry Michael , Power Hannah , Du Jiabi , Tucker Tobias , Cienfuegos Rodrigo , Catalán Patricio , Hanslow David 
Published: November 2024

Abstract

Intermittently open/closed estuaries provide important ecosystem services but are often overlooked in coastal–catchment research and management. These estuaries are highly vulnerable to human/climate disturbances due to their tendency to close off from the ocean, yet their processes/dynamics remain under-researched. This study maps the global distribution of at least 2,245 intermittent estuaries, whose catchments currently support 55 million people, with projections rising to 101 million by 2100. Assessing three decades of scholarly articles indicated that only 7% of these sites have been studied. Academic literature on intermittent estuaries accounted for 0.5% of the total literature on all estuaries, despite these systems representing 4–5% of the estimated total number of global estuaries. Significant research gaps exist in Asia, South America, and Africa, where the largest, most susceptible populations reside. 90% of the existing research on intermittent estuaries is conducted in (southern) Africa (42%), Oceania (35%), and North America (14%), predominantly through domestic efforts. From 1992 to 2023, 60% of the research focused on physio-chemical and eco-hydro-geomorphological topics, with minimal attention to ecosystem services, climatic/human disturbances, and management. Our assessment underscores the need for increased focus on intermittent estuaries and suggests strategies to promote international collaborations, including leadership from intergovernmental organisations.
The Role of Moracin M in Promoting Hair Growth: Insights into Mechanisms of WNT/β- Catenin Pathway Activation and Angiogenesis Enhancement in Human Dermal Papilla Cells
Authors: Chang BoYoon , Hwang Yuri , Kim In , Park Hyungmin , Kim Yonghwan , Kim SungYeon 
Published: November 2024

Abstract

Hair follicle growth depends on the intricate interaction of cells within the follicle and its vascular supply. Current FDA-approved treatments like minoxidil have limitations, including side effects and the need for continuous use. Moracin M, a compound from Moraceae family, was investigated for its effects on hair growth and vascular regeneration. In our study, Moracin M significantly increased cell proliferation in human dermal papilla cells (hDPCs) during both the anagen and catagen phases and promoted cell migration in human umbilical vein endothelial cells (HUVECs) without cytotoxicity at concentrations up to 50 µM. Mechanistic analysis revealed that moracin M enhanced GSK-3β phosphorylation and increased non-phospho β-catenin levels, activating Wnt signaling and upregulating transcription factors LEF, TCF, and AXIN2. This resulted in elevated levels of growth factors VEGF, FGF2, KGF, HGF and MYC in hDPCs, effects comparable to those of minoxidil. Additionally, moracin M significantly increased protein and mRNA levels of VEGF, FGF2, and KGF in hDPCs under IFN-γ-induced inflammatory conditions. Moracin M treatments also resulted in notable wound width reductions in a dose-dependent manner. Further investigation showed that moracin M stimulated MMP-2 and MMP-9 expression while having no effect on MMP-7 levels. These findings indicate that moracin M significantly enhances hair growth through the promotion of cell proliferation and angiogenesis, particularly via the activation of the Wnt signaling pathway in dermal papilla cells, presenting it as a promising therapeutic alternative to current treatments.