bluuebunny/crossref_metadata_2025_split huggingface dataset:
The power of odour volatiles from unifloral melipona honey evaluated by gas chromatography–olfactometry Osme techniques
Authors: Costa Ana CV , Garruti Deborah S , Madruga Marta S 
Published: February 2019

AbstractBackgroundThe quality and power of each odorous volatile present in the headspace of unifloral honey velame branco (Croton heliotropiifolius Kunth) prepared by stingless uruçu (Melipona scutellaris Latrelle) were evaluated using the gas chromatography–olfactometry Osme technique. Five trained panellists evaluated the sensory chromatographic effluent in three replications, and the results were integrated by SCDTI software.ResultsOf the 42 compounds identified in the chromatogram, 17 odorous stimuli were detected by the sensory panel. Among these compounds, the most odoriferous impacts were pentanoate acetate ‘ripe fruit’, safranal green, medicinal plant and methyl eugenol ‘clove, tea’. The furaneol compounds, hotrienol and benzaldehyde showed aroma notes associated with honey.ConclusionThese results are important since, in addition to revealing the presence of compounds that are not identified by other analytical methods, they can also assist in the verification of monofloral honeys compliance. © 2019 Society of Chemical Industry
Modeling precursors of impulsive tourist shopping behavior: Evidence from long‐haul Chinese outbound tourists
Authors: Meng Fang , Zhang Pei , Li Hengyun , So Kevin Kam Fung 
Published: February 2019

AbstractStudies have examined tourism shopping in various aspects, but scarce research has specifically focused on impulsive shopping behavior of tourists, which is of particular relevance in the tourism settings. This study addresses this paucity by examining factors influencing impulsive tourist shopping urge and purchase from the aspects of tourist internal attribute, social influence, and product attribute. By examining Chinese long‐haul tourists traveled outside Asia, the empirical results suggest that (a) impulsive trait and hedonic and materialistic tendency of tourists lead to impulsive urge and engagement in actual impulsive buying; (b) shopping companion/social influences from family and relatives, peers (friends/colleagues), and shop assistants also affect the impulsive shopping urge; (c) product brand variety shows significant effect on impulsive urge, whereas the influence of product price is insignificant; and (d) traveling with a shopping list or not moderates the relationship between impulsive urge and actual impulsive purchase. The study advances the theoretical understanding of tourist impulsive shopping behavior and provides marketing/managerial insights into Chinese overseas tourism market.
Long‐Term Measures of Dyslipidemia, Inflammation, and Oxidative Stress in Rats Fed a High‐Fat/High‐Fructose Diet
Authors: Feillet‐Coudray Christine , Fouret Gilles , Vigor Claire , Bonafos Béatrice , Jover Bernard , Blachnio‐Zabielska Agnieszka , Rieusset Jennifer , Casas François , Gaillet Sylvie , Landrier Jean Francois , Durand Thierry , Coudray Charles 
Published: February 2019

AbstractInflammation and oxidative stress are thought to be involved in, or associated with, the development of obesity, dyslipidemia, hepatic steatosis, and insulin resistance. This work was designed to determine the evolution of inflammation and oxidative stress during onset and progression of hepatic steatosis and glucose intolerance. Seventy‐five male Wistar rats were divided to control and high‐fat high‐fructose (HFHFr) groups. A subgroup of each group was sacrificed at 4, 8, 12, 16, and 20 weeks. HFHFr‐fed rats exhibited overweight, glucose intolerance, and hepatic steatosis with increased contents of hepatic diacylglycerols and ceramides. The HFHFr diet increased hepatic interleukin 6 (IL‐6) protein and adipose tissue CCL5 gene expression and hepatic nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity but not mitochondrial reactive oxygen species (ROS) production. The HFHFr diet decreased plasma and liver levels of isoprostanoid metabolites as well as plasma thiobarbituric acid‐reactive substance (TBARS) levels. Hepatic glutathione content was decreased with a moderate decrease in superoxide dismutase (SOD) and glutathione peroxidase (GPx) with the HFHFr diet. Overall, HFHFr diet led to hepatic lipid accumulation and glucose intolerance, which were accompanied by only moderate inflammation and oxidative stress. Most of these changes occurred at the same time and as early as 8 or 12 weeks of diet treatment. This implies that oxidative stress may be the result, not the cause, of these metabolic alterations, and suggests that marked hepatic oxidative stress should probably occur at the end of the steatotic stage to result in frank insulin resistance and steatohepatitis. These findings need to be further evaluated in other animal species as well as in human studies.
Characterization of Collagen/Lipid Nanoparticle–Curcumin Cryostructurates for Wound Healing Applications
Authors: Laghezza Masci Valentina , Taddei Anna‐Rita , Courant Thomas , Tezgel Ozgül , Navarro Fabrice , Giorgi Franco , Mariolle Denis , Fausto Anna‐Maria , Texier Isabelle 
Published: February 2019

AbstractCurcumin‐loaded collagen cryostructurates have been devised for wound healing applications. Curcumin displays strong antioxidant, antiseptic, and anti‐inflammatory properties, while collagen is acknowledged for promoting cell adhesion, migration and differentiation. However, when curcumin is loaded directly into collagen hydrogels, it forms large molecular aggregates and clogs the matrix pores. A double‐encapsulation strategy is therefore developed by loading curcumin into lipid nanoparticles (LNP), and embedding these particles inside collagen scaffolds. The resulting collagen/LNP cryostructurates have an optimal fibrous structure with ≈100 µm average pore size for sustaining cell migration. Results show that collagen is structurally unaltered and that nanoparticles are homogeneously distributed amidst collagen fibers. Hydrogels soaked in saline buffer release about 20 to 30% of their nanoparticles content within 24 h, while achieved 100% release after 25 days. When exposed to NIH 3T3 fibroblasts, these hydrogels provide a satisfactory scaffold for cell interaction as early as 4 h after seeding, with no cytotoxic counter effect. These positive features make the collagen/lipid cryostructurates a promising material for further use in wound healing.
A monolithic approach to fluid‐structure interaction based on a hybrid Eulerian‐ALE fluid domain decomposition involving cut elements
Authors: Schott B. , Ager C. , Wall W.A. 
Published: February 2019

SummaryA novel method for complex fluid‐structure interaction (FSI) involving large structural deformation and motion is proposed. The new approach is based on a hybrid fluid formulation that combines the advantages of purely Eulerian (fixed‐grid) and arbitrary Lagrangian‐Eulerian (ALE) moving mesh formulations in the context of FSI. The structure, as commonly given in Lagrangian description, is surrounded by a fine resolved layer of fluid elements based on an ALE‐framework. This ALE‐fluid patch, which is embedded in a Eulerian background fluid domain, follows the deformation and motion of the structural interface. This approximation technique is not limited to finite element methods but can also be realized within other frameworks like finite volume or discontinuous Galerkin methods. In this work, the surface coupling between the two disjoint fluid subdomains is imposed weakly using a stabilized Nitsche's technique in a cut finite element method (CutFEM) framework. At the fluid‐solid interface, standard weak coupling of node‐matching or nonmatching finite element approximations can be utilized. As the fluid subdomains can be meshed independently, a sufficient mesh quality in the vicinity of the common fluid‐structure interface can be assured. To our knowledge, the proposed method is the only method (despite some overlapping domain decomposition approaches that suffer from other issues) that allows for capturing boundary layers and flow detachment via appropriate grids around largely moving and deforming bodies. In contrast to other methods, it is possible to do this, eg, without the necessity of costly remeshing procedures. A clear advantage over existing overlapping domain decomposition methods consists in the sharp splitting of the fluid domain, which comes along with improved convergence behavior of the resulting monolithic FSI system. In addition, it might also help to save computational costs as now background grids can be much coarser. Various FSI‐cases of rising complexity conclude the work. For validation purpose, results have been compared to simulations using a classical ALE‐fluid description or purely fixed‐grid CutFEM‐based schemes.
Urolithin A, a Gut Metabolite, Improves Insulin Sensitivity Through Augmentation of Mitochondrial Function and Biogenesis
Authors: Toney Ashley Mulcahy , Fan Rong , Xian Yibo , Chaidez Virginia , Ramer‐Tait Amanda E. , Chung Soonkyu 
Published: February 2019

ObjectiveUrolithin A (UroA) is a major metabolite of ellagic acid produced following microbial catabolism in the gut. Emerging evidence has suggested that UroA modulates energy metabolism in various cells. However, UroA’s physiological functions related to obesity and insulin resistance remain unclear.MethodsMale mice were intraperitoneally administrated either UroA or dimethyl sulfoxide (vehicle) along with a high‐fat diet for 12 weeks. Insulin sensitivity was evaluated via glucose and insulin tolerance tests and acute insulin signaling. The effects of UroA on hepatic triglyceride accumulation, adipocyte size, mitochondrial DNA content, and proinflammatory gene expressions were determined. The impact of UroA on macrophage polarization and mitochondrial respiration were assessed in bone marrow–derived macrophages.ResultsAdministration of UroA (1) improved systemic insulin sensitivity, (2) attenuated triglyceride accumulation and elevated mitochondrial biogenesis in the liver, (3) reduced adipocyte hypertrophy and macrophage infiltration into the adipose tissue, and (4) altered M1/M2 polarization in peritoneal macrophages. In addition, UroA favored macrophage M2 polarization and mitochondrial respiration in bone marrow‐derived macrophages.ConclusionsUroA plays a direct role in improving systemic insulin sensitivity independent of its parental compounds. This work supports UroA’s role in the metabolic benefits of ellagic acid–rich foods and highlights the significance of its microbial transformation in the gut.
Ring‐Stacking Water Clusters: Morphology and Stabilities
Authors: Yang Liu , Ji Hanyang , Liu Xiaojie , Lu Wen‐Cai 
Published: February 2019

AbstractThe structures and interaction energies of water clusters with ring stacking motifs are studied by using ab initio calculations. The structures of the water clusters are constructed by stacking either single rings or multi‐rings of tetramer, pentamer, and hexamer. We found that, in the single‐ring‐stacking motif, the most stable isomers exhibit an alternative clockwise‐anticlockwise stacking pattern. We also show that four‐layer single‐ring‐stacking isomers are not energetically favorable in comparison with those of two‐layer multi‐ring‐stacking isomers. The relative stability of the isomers is also analyzed in terms of H‐bond strength and elastic distortions of the water molecules.
Properties and Structure of ADN‐Prills
Authors: Heintz Thomas , Herrmann Michael J. 
Published: February 2019

AbstractAmmonium dinitramide (ADN) is the upcoming oxidizer for solid propellants of the current decade. Spherical ADN particles, so‐called ADN‐prills, are a key product for the development of environmentally friendly and low‐signature solid propellants for defence and space applications. At the moment the particle size and the spherical shape of the ADN‐prills can be controlled by the emulsion crystallization process or by spray congealing processes. In this work the crystal structure and the thermal‐ and mechanical stability of ADN‐prills were investigated. By temperature resolved X‐ray diffraction measurements new insights about temperature depending crystalline structures inside of ADN‐prills could be gained. Anisotropic crystal properties could be detected at certain temperature ranges between ambient temperature and the melting point of ADN at 93 °C. Due to the unexpected material properties of molten ADN droplets a surprising correlation between process parameters, particle size, crystal structure, thermal stability and mechanical stability was found. This leads to a more profound knowledge about the crystal structure of ADN‐prills and gives the opportunity to produce enhanced ADN particles for propellant applications.
Human Fibrinogen for Maintenance and Differentiation of Induced Pluripotent Stem Cells in Two Dimensions and Three Dimensions
Authors: Gandhi Jarel K. , Knudsen Travis , Hill Matthew , Roy Bhaskar , Bachman Lori , Pfannkoch-Andrews Cynthia , Schmidt Karina N. , Metko Muriel M. , Ackerman Michael J. , Resch Zachary , Pulido Jose S. , Marmorstein Alan D. 
Published: February 2019

Abstract
Human fibrin hydrogels are a popular choice for use as a biomaterial within tissue engineered constructs because they are biocompatible, nonxenogenic, autologous use compatible, and biodegradable. We have recently demonstrated the ability to culture induced pluripotent stem cell (iPSC)-derived retinal pigment epithelium on fibrin hydrogels. However, iPSCs themselves have relatively few substrate options (e.g., laminin) for expansion in adherent cell culture for use in cell therapy. To address this, we investigated the potential of culturing iPSCs on fibrin hydrogels for three-dimensional applications and further examined the use of fibrinogen, the soluble precursor protein, as a coating substrate for traditional adherent cell culture. iPSCs successfully adhered to and proliferated on fibrin hydrogels. The two-dimensional culture with fibrinogen allows for immediate adaption of culture models to a nonxenogeneic model. Similarly, multiple commercially available iPSC lines adhered to and proliferated on fibrinogen coated surfaces. iPSCs cultured on fibrinogen expressed similar levels of the pluripotent stem cell markers SSea4 (98.7% ± 1.8%), Oct3/4 (97.3% ± 3.8%), TRA1-60 (92.2% ± 5.3%), and NANOG (96.0% ± 3.9%) compared with iPSCs on Geltrex. Using a trilineage differentiation assay, we found no difference in the ability of iPSCs grown on fibrinogen or Geltrex to differentiate to endoderm, mesoderm, or ectoderm. Finally, we demonstrated the ability to differentiate iPSCs to endothelial cells using only fibrinogen coated plates. On the basis of these data, we conclude that human fibrinogen provides a readily available and inexpensive alternative to laminin-based products for the growth, expansion, and differentiation of iPSCs for use in research and clinical cell therapy applications. Stem Cells Translational Medicine 2019;8:512–521
Concise Review: Update on Retinal Pigment Epithelium Transplantation for Age-Related Macular Degeneration
Authors: Zarbin Marco , Sugino Ilene , Townes-Anderson Ellen 
Published: February 2019

AbstractRetinal cell therapy can have the objectives of rescue (i.e., modulation of metabolic abnormalities primarily for sight preservation) as well as replacement (i.e., replace cells lost due to injury or disease for sight restoration as well as preservation). The first clinical trials of retinal pigment epithelium (RPE) transplantation for vision-threatening complications of age-related macular degeneration (AMD) have begun with some preliminary signs of success (e.g., improvement in vision in some patients, anatomic evidence of transplant-host integration with some evidence of host photoreceptor recovery, long-term survival of autologous induced pluripotent stem cell-derived RPE transplants without immune suppression) as well as limitations (e.g., limited RPE suspension survival in the AMD eye, limited tolerance for long-term systemic immune suppression in elderly patients, suggestion of uncontrolled cell proliferation in the vitreous cavity). RPE survival on aged and AMD Bruch’s membrane can be improved with chemical treatment, which may enhance the efficacy of RPE suspension transplants in AMD patients. Retinal detachment, currently used to deliver transplanted RPE cells to the subretinal space, induces disjunction of the first synapse in the visual pathway: the photoreceptor-bipolar synapse. This synaptic change occurs even in areas of attached retina near the locus of detachment. Synaptic disjunction and photoreceptor apoptosis associated with retinal detachment can be reduced with Rho kinase inhibitors. Addition of Rho kinase inhibitors may improve retinal function and photoreceptor survival after subretinal delivery of cells either in suspension or on scaffolds.
The association between stressful life events and respiratory infections during the first 4 years of life: The Environmental Determinants of Diabetes in the Young study
Authors: Roth Roswith , Lynch Kristian , Hyöty Heikki , Lönnrot Maria , Driscoll Kimberly A. , Bennett Johnson Suzanne ,   the TEDDY Study Group
Published: February 2019

AbstractThe aim of this study was to conduct a prospective analysis of the association between negative life events (NLEs) and respiratory infections in children genetically at risk for islet autoimmunity (IA) and type 1 diabetes (T1D). Long‐ and short‐term temporal associations between NLEs and rate of respiratory infection episodes (RIEs) in 5,618 children in The Environmental Determinants of Diabetes in the Young study for at least 1 up to 4 years were analysed. All models were adjusted for demographic, day care, season of infection, and psychosocial factors associated with the rate of child RIEs between study visits. The rate of child RIEs was 26% higher in Europe (Sweden, Finland, Germany) than in the United States (rate ratio [RR] = 1.26, p < 0.001). However, the percentage of child NLEs (odds ratio [OR] = 1.18, p < 0.001) and mother NLEs (OR = 1.83, p < 0.001) was higher in the United States compared with Europe. In both continents (Europe, RR = 1.12, p < 0.001; United States, RR = 1.07, p = 0.006), high child cumulative NLEs (>1 NLE per year since study inception) was significantly associated with an increased rate of child RIEs. This large‐scale prospective study confirms observations that stress may increase the susceptibility for infections in paediatric populations and suggests at least one mechanism by which stress could increase risk for IA and T1D in genetically at risk children.
Systematic position of the enigmatic liverwort Mizutania (Mizutaniaceae, Marchantiophyta) inferred from molecular phylogenetic analyses
Authors: Masuzaki Hiroaki , Shimamura Masaki , Furuki Tatsuwo , Tsubota Hiromi , Yamaguchi Tomio , Majid Haji Mohamed Abdul , Deguchi Hironori 
Published: February 2019

AbstractPhylogenetic analyses of liverworts using chloroplast rbcL and rps4 sequences were performed with special reference to the enigmatic thalloid liverwort, Mizutania riccardioides. The results showed that Mizutania is nested within leafy liverwort family Calypogeiaceae (subclass Jungermanniidae), thereby refuting the traditional interpretation that Mizutania is a member of the simple thalloid liverworts (subclass Metzgeriidae) related to Aneuraceae. Male plants with three ranked bracts, which are newly discovered in this study, also show a close morphological affinity to those of leafy liverworts. Parallel evolution of the flattened gametophyte seems to have occurred sporadically in the Jungermanniidae in association with adaptation to prostrate growth on the ground or on living leaves. The unistratose thalloid features of Mizutania are interpreted as an extremely reduced or highly specialized form of a creeping leafy liverwort. The rudimentary sexual branches which develop on the margins of the thallus are similar in many characteristics to those of Calypogeiaceae. We reduce the monotypic family Mizutaniaceae to a synonym of Calypogeiaceae.
A revised classification of Santalales
Authors: Nickrent Daniel L. , Malécot Valéry , Vidal-Russell Romina , Der Joshua P. 
Published: February 2019

AbstractWe present here a revised classification of Santalales, an angiosperm order that contains 18 families, 160 genera, and over 2200 species. Both nonparasitic and parasitic flowering plants occur in the traditionally circumscribed family Olacaceae whereas all other families are composed entirely of parasites. The five evolutionary radiations of aerial parasitism produced mistletoes that constitute most of the generic and specific diversity seen in the order. This classification, although based primarily upon results from molecular phylogenetic investigations, brings together all currently available information that contributes to our understanding of relationships among these plants. Monophyletic groups (clades) obtained from molecular analyses were named using a Linnaean ranked system. Four new families are named that formerly resided in Santalaceae s.l.: Amphorogynaceae, Cervantesiaceae, Comandraceae, and Nanodeaceae. A new tribal and subtribal classification for Loranthaceae is presented where nine new subtribe names are proposed.
Biogeography of high‐mountain plants in the Carpathians: An emerging phylogeographical perspective
Authors: Ronikier Michał 
Published: February 2019

AbstractThe Carpathians belong to the main elements of the European Alpine System and have an important biogeographical location between the Balkan ranges in the South, the Alps in the West and the Scandinavian range in the North. However, until recently this area was rarely included in detailed phylogeographical studies that could bring insights into its biogeographical history, links with other mountain ranges and contemporary genetic structure of populations. Here, available phylogeographical studies on high‐mountain plants that include data concerning the Carpathians are reviewed in order to (1) discuss regional phylogeographical structure and divergence of the Carpathian populations from those in other European mountain ranges, and (2) outline further perspectives of the Carpathian phylogeography. Analysis of available studies revealed the complexity of the biogeographical history of high‐mountain plants. The studies show a deep phylogeographical structure in the Carpathians, mostly concurring with classical biogeographical boundaries, and suggesting a long‐term isolation and restricted gene flow between the main Carpathian regions. For some species, though, recent dispersal events among isolated mountain ranges were detected. Such contrasting patterns were found at a larger geographical scale as well (e. g., between the Carpathians and the Alps). Several examples suggest the importance of the Carpathians in migration of arctic‐alpine plants from the East and towards the North. In most reviewed studies, however, the Carpathians are only marginally represented and detailed intraspecific studies based on dense population coverage in all disjunct areas of species' ranges are clearly needed to obtain reliable information and confirm the preliminary phylogeographical patterns emerging from the overview presented here.