bluuebunny/crossref_metadata_2025_split huggingface dataset:
An efficient high‐speed countercurrent chromatography method for preparative separation of javanicin from Fusarium solani, a fungus isolated from the fruiting body of the mushroom Trametes trogii
Authors: Guo Ruyi , Cai Xu , Li Qian , Huang Yun , Chen Benke , Guan Ping , Tang Jintian , Zou Xianwei 
Published: May 2019

AbstractTo develop an efficient method for large preparation of javanicin from Fusarium solani, a rapid and simple method by high‐speed countercurrent chromatography was established based on average polarity (P′ values) and partition coefficients (K values) of crude samples. A suitable solvent system for high‐speed countercurrent chromatography was selected from many possible biphasic solvent systems. HSCCC was successfully applied to separate and purify javanicin, the main bioactive component of solid cultures of the fungus F. solani isolated from the fruiting body of Trametes trogii, with petroleum ether–ethyl acetate–methanol–water (4:3:2:1, v/v) as solvent system. A total amount of 40.6 mg of javanicin was obtained from 100 mg crude sample. The purity of javanicin was 92.2% with a recovery of 95.1%, as determined by high‐performance liquid chromatrography. The molecular structure was identified primarily by NMR and MS methods. The results indicated that high‐speed countercurrent chromatography could be a powerful technology for separating naphthoquinones from the solid cultures of the fungus F. solani. It is also of significance that the separation of javanicin from natural source was carried out for the first time utilizing high‐speed countercurrent chromatography.
Direct costs of both inpatient and outpatient care for all type cancers: The evidence from Beijing, China
Authors: Yin Xuejun , Xu Yue , Man Xiaowei , Liu Liming , Jiang Yan , Zhao Liying , Cheng Wei 
Published: May 2019

AbstractBackgroundCancer is a major public health issue worldwide. The cost of cancer care imposes a substantial economic burden on society and patient, but it has not been thoroughly studied in China. This study aimed to describe direct cost and cost elements of all cancer types by different beneficial characteristics.MethodsThe research was a retrospective observational study based on inpatient and outpatient records with a primary diagnosis of cancer from 31 hospitals in 2016. Total cost and cost per time were analyzed by cancer type, sources (prescription medicines, consumables fee for diagnosis and surgery, and other health services), and beneficial characteristics (gender and age).ResultsA total of 30 224 eligible inpatient admissions and 485 391 outpatient visits were identified during the study period. Inpatient care costs account for 58.6% cancer treatment costs. Nearly 70% of the total expenditure is spent on patients aged 50‐79 years. Lung cancer had the highest economic cost (15% of overall cancer costs), followed by breast cancer (12%), and colorectal cancer (10%). Anticancer drug cost accounted a large proportion in both inpatient (37.7%) and outpatient care (64.6%). The average cost per inpatient admission was estimated to be $4590.1 (5621.9), ranging from $1157.7 (1349.8) for testis cancer to $7975 (7343.9) for stomach cancer. The regression analyses revealed that length of hospital stay, cancer type, age, payment type, and hospital level were highly correlated with the expenditure per admission (P < 0.001).ConclusionsThe cancer care cost is substantial and varies with cancer type. Our findings provide important information for health service planning, allowing more efficient allocation of health resources for the care of people with cancer.
LncRNA BDNF‐AS suppresses colorectal cancer cell proliferation and migration by epigenetically repressing GSK‐3β expression
Authors: Zhi Hui , Lian Jiayu 
Published: May 2019

This study was designed to investigate the molecular mechanism and biological roles of long non‐coding RNA (lncRNA) brain‐derived neurotrophic factor antisense (BDNF‐AS) in colorectal cancer (CRC). The quantitative real‐time PCR (qRT‐PCR) and western blotting were performed to detect the expressions of lncRNA BDNF‐AS and glycogen synthase kinase‐3β (GSK‐3β) in human CRC tissues and cell lines. The cell proliferation, transwell migration, and invasion assays were carried out to evaluate the effect of lncRNA BDNF‐AS on the growth of CRC cells. RNA pull‐down and RNA immunoprecipitation (RIP) assays were conducted to confirm the interaction between lncRNA BDNF‐AS and enhancer of Zeste Homologue 2 (EZH2). Chromatin immunoprecipitation (ChIP) assay was used to verify the enrichment of EZH2 and histone H3 lysine 27 trimethylation (H3K27me3) in the promoter region of GSK‐3β in CRC cells. LncRNA BDNF‐AS expression was significantly decreased, while GSK‐3β was highly expressed in human CRC tissues and cell lines. Moreover, lncRNA BDNF‐AS induced inhibition of proliferation, migration, and invasion of CRC cells via inhibiting GSK‐3β expression. Mechanistically, BDNF‐AS led to GSK‐3β promoter silencing in CRC cells through recruitment of EZH2. In conclusion, lncRNA BDNF‐AS functioned as an oncogene in CRC and shed new light on lncRNA‐directed therapeutics in CRC.Significance of the studyLncRNA BDNF‐AS is recently reported to be remarkably downregulated in a variety of tumours and served as a tumour suppressor. However, the functions and underlying mechanism of lncRNA BDNF‐AS in CRC pathogenesis have not been reported yet. Our study is the first to demonstrate the effect of lncRNA BDNF‐AS in CRC and revealed that lncRNA BDNF‐AS expression is negatively correlated with the aggressive biological behaviour of CRC. Further investigation demonstrated that lncRNA BDNF‐AS functioned as a tumour suppressor in CRC progression by suppressing GSK‐3β expression through binding to EZH2 and H3K27me3 with the GSK‐3β promoter, shedding light on the diagnosis and therapy for CRC.
Blockade of AT1 receptor restore the migration of vascular smooth muscle cells in high sodium medium
Authors: Brun Bruna F. , Strela Felipe B. , Berger Rebeca C. M. , Melo Stéphano F. S. , de Oliveira Edilamar M. , Barauna Valério G. , Vassallo Paula F. 
Published: May 2019

AbstractThe present study aimed to test the hypothesis that increased sodium concentration affects the migratory phenotype of vascular smooth muscle cells (VSMCs) independently of the haemodynamic factors. Cell migration was evaluated by wound‐healing assay under the following conditions: high sodium (HS, 160 mM) and control (CT, 140 mM). Cell viability was assessed by annexin V and propidium iodide labeling. Cyclooxygenase‐2 (COX‐2) gene expression was analysed by reverse transcription polymerase chain reaction. ERK1/2 phosphorylation was assessed by western blot. Exposure of VSMCs to HS reduced migration, and AT1R blockade prevented this response. HS increased COX‐2 gene expression, and COX‐2 blockade prevented the reduction in VSMC migration induced by HS. HS also increased ERK1/2 phosphorylation, and ERK1/2 inhibition recovered VSMC migration as well as blocked COX‐2 gene expression. The TXA2 receptor blocker, but not the prostacyclin receptor blocker, prevented the HS‐induced VSMCs migration decrease. HS reduces the migration of VSMCs by increasing COX‐2 gene expression via AT1R‐ERK1/2 phosphorylation. In addition, increased COX‐2 by HS seems to modulate the reduction of VSMCs migration by the TXA2 receptor.
Explantation and reimplantation of a breast implant to facilitate minimally invasive mitral valve surgery and cryoablation of atrial fibrillation
Authors: Cetinkaya Ayse , Borsche André , Hein Stefan , Bramlage Peter , Schoenburg Markus , Richter Manfred 
Published: May 2019

AbstractClinicians can feel confident about performing mitral repair/replacement in patients who have previously undergone mammoplasty. It may also have applications in performing atrial septal defect closure, Maze procedures for atrial fibrillation, and tricuspid valve surgery in patients with breast implants.
Rational Design of Transition Metal Co‐doped Ceria Catalysts for Low‐Temperature CO Oxidation
Authors: Kim Hyung Jun , Lee Geonhee , Jang Myeong Gon , Noh Kyung‐Jong , Han Jeong Woo 
Published: May 2019

AbstractThe front cover artwork for Issue 09/2019 is provided by researchers from Computational Catalysis and Emerging Materials Laboratory (CCEL) at Pohang University of Science and Technology and the University of Seoul (Republic of Korea). The image shows rational design of transition metal co‐doped ceria catalyst to be used for automobile exhaust removal. See the Full Paper itself at https://doi.org/10.1002/cctc.201900178.
Pyrrole‐Based π‐System–PtII Complexes: Chiroptical Properties and Excited‐State Dynamics with Microsecond Triplet Lifetimes
Authors: Hirata Goki , Kobayashi Yoichi , Sato Ryuma , Shigeta Yasuteru , Yasuda Nobuhiro , Maeda Hiromitsu 
Published: May 2019

AbstractHeteroleptic PtII complexes comprising π‐extended dipyrrins and 2‐phenylquinoline were prepared. Single‐crystal X‐ray analysis disclosed the stepped conformations of two ligand moieties in these PtII complexes. The enantiomers could be separated by HPLC and their configurations were determined from CD spectroscopy results and TD‐DFT calculations. Transient absorption measurements revealed excited‐state dynamics characterized by fast intersystem crossing and microsecond‐order triplet‐state lifetimes.
An efficient similarity join approach on large‐scale high‐dimensional data using random projection
Authors: Ma Youzhong , Zhang Ruiling , Jia Shijie , Zhang Yongxin , Meng Xiaofeng 
Published: May 2019

SummarySimilarity join on large‐scale high‐dimensional data faces major challenges because of the data scale and the cure of dimensionality. Random projection with p‐stable distribution can reduce the high‐dimensional data form d‐dimension to k‐dimension (k ≪ d), the distance of the data in k‐dimensional space can be used to filter out as many data pairs as possible at relative low cost. Based on the above idea, we proposed two novel approaches to deal with large‐scale high‐dimensional data similarity join: projection‐based similarity join (PromSimJ) algorithm and projection space partitioning–based similarity join (ProSPSimJ) algorithm. The comprehensive experiments were performed to test the performance of the above methods. We also compared the performance of the above methods with that of the naive method block nested loop join. The final experimental results prove that our approaches have much better performance and good scalability.
Willingness of individuals with eating disorders to participate in health research
Authors: Nutley Sara , Varma Deepthi , Chen Xinguang , Striley Catherine W. 
Published: May 2019

AbstractBackground/AimsThis study examines the association between eating disorders (EDs) and willingness to participate in health research studies among community members.Materials & MethodData were collected from Health Street, a University of Florida community engagement initiative which aims to reduce disparities in healthcare and health research by direct engagement of community members.ResultsAmong 8,226 community members, 3.9% (n = 324) reported a lifetime ED. For all six types of health research studies queried, individuals with a lifetime ED reported a higher willingness to participate in health research compared to individuals without a history. After adjusting for selected covariates, individuals with ED were significantly more likely than individuals without ED to say they would be willing to volunteer for research studies that: ask questions about health (OR: 7.601, 95% CI: [1.874, 30.839]); require an overnight stay in a hospital (OR: 2.041, 95% CI: [1.442, 2.889]); and provide no remuneration (OR: 1.415, 95% CI: [1.022, 1.958]). Furthermore, when compared to individuals with anxiety or depression, individuals with ED reported increased interest in research participation and increased willingness to participate in most types of research studies assessed. After stratifying by gender and race, we observed few differences in willingness to participate in research among individuals with ED.DiscussionThese findings contribute to our current understanding of participant recruitment and enrollment in ED health research. Underrepresented populations who often do not seek treatment for EDs endorsed a high willingness to participate.ConclusionFuture studies will likely benefit from including community members in ED research.
Effects of habitat area and spatial configuration on biodiversity in an experimental intertidal community
Authors: Loke Lynette H. L. , Chisholm Ryan A. , Todd Peter A. 
Published: May 2019

AbstractIsolating the effects of fragmentation per se (i.e., spatial configuration of habitat patches) on species richness is an ongoing challenge as habitat configuration often covaries with the amount of habitat. Consequently, there is a lack of experimental evidence for configurational effects on species richness in the whole landscape. Here, we developed a novel experimental system for testing the independent and interactive effects of habitat area and configuration on tropical intertidal species richness. Our results confirmed the expectation that average species richness would increase monotonically with habitat area. More intriguingly, we found mixed evidence for a non‐monotonic relationship between species richness and fragmentation per se, with the highest richness at intermediate fragmentation configuration, that is, when habitat tiles were placed in a “several‐small” configuration. The effect of habitat configuration was not due to passive sampling (since area was controlled for), variation in total individual abundance, or niche specialization of species to different landscape configurations. We postulate that a combination of processes, including local negative density dependence and dispersal limitation, could give rise to the observed pattern. We emphasize the importance of considering configurational effects on biodiversity at broader spatial scales and for more experimental research to delve into the mechanisms driving the patterns seen here.
The origin and persistence of alpine vernal ponds in mineral soils
Authors: Harrison‐Day Violet , Atkinson Joe , Kirkpatrick Jamie B. 
Published: May 2019

AbstractLittle is known of the processes that create and maintain vernal ponds in mineral soils in alpine environments. On the Central Plateau, Tasmania, we tested the hypotheses that vernal pond complexes on mineral soils formed in response to the underlying topography of a glacio‐fluvial plain; relate to present day topography; resulted from past damming by organic accumulation; are moulded by wind. The underlying topography did not relate to the surface ponds, nor were they on steeper slopes than adjacent areas without ponds. The morphology of the ponds and the morphological and edaphic characteristics of the pond complexes and adjacent areas are consistent with an origin by organic material damming. The strongest winds orientate most ponds, rather than the aspect of the slope. Sediments were preferentially caught on sticky traps to the northeast of the ponds, away from fierce prevailing southwesterly winds. Temperature measurements and fortnightly observation showed non‐concordant patterns of variation in water levels in the ponds. We deduce that the complexes of vernal ponds may have formed in previous moister conditions more favourable to organic matter accumulation, possibly in the early Holocene, and are maintained by a faster rate of accumulation of mineral and organic particles in the tussock grassland adjacent to the ponds than in the ponds themselves. © 2019 John Wiley & Sons, Ltd.
Decadal morphological evolution of the mouth zone of the Yangtze Estuary in response to human interventions
Authors: Zhu Chunyan , Guo Leicheng , van Maren Dirk Sebastiaan , Tian Bo , Wang Xianye , He Qing , Wang Zheng Bing 
Published: May 2019

AbstractThe morphology of the Yangtze Estuary has changed substantially at decadal time scales in response to natural processes, local human interference and reduced sediment supply. Due to its high sediment load, the morphodynamic response time of the estuary is short, providing a valuable semi‐natural system to evaluate large‐scale estuarine morphodynamic responses to interference. Previous studies primarily addressed local morphologic changes within the estuary, but since an overall sediment balance is missing, it remains unclear whether the estuary as a whole has shifted from sedimentation to erosion in response to reduced riverine sediment supply (e.g. resulting from construction of the Three Gorges Dam). In this paper we examine the morphological changes of two large shoals in the mouth zone (i.e. the Hengsha flat and the Jiuduan shoal) using bathymetric data collected between 1953 and 2016 and a series of satellite images. We observe that the two shoals accreted at different rates before 2010 but reverted to erosion thereafter. Human activities such as dredging and dumping contribute to erosion, masking the impacts of sediment source reduction. The effects of local human intervention (such as the construction of a navigation channel) are instantaneous and are likely to have already resulted in new dynamic equilibrium conditions. The morphodynamic response time of the mouth zone to riverine sediment decrease is further suggested to be >30 years (starting from the mid‐1980s). Accounting for the different adaptation time scales of various human activities is essential when interpreting morphodynamic changes in large‐scale estuaries and deltas. © 2019 John Wiley & Sons, Ltd.
Plasma Cholinesterase Activity in Wild Birds from Undisturbed Woodlands in the Central Monte Desert
Authors: Quero Arnoldo Ángel Martín , Zarco Agustín , Landa Florencia Belén , Gorla Nora Bibiana María 
Published: May 2019

Abstract
Plasma cholinesterase activity is a biomarker sensitive to the effect of organophosphate and carbamate pesticides, and its enzymatic levels have been previously unknown for most of the wild birds analyzed in the present study. Our objectives were to establish plasma acetylcholinesterase levels in songbirds of 2 undisturbed sites in the central Monte Desert (Argentina). We also examined the influence on cholinesterase activity of age, sex, body condition, feeding and migratory habits, and species. One hundred and sixty-five wild birds belonging to 26 species were studied. The values obtained for acetylcholinesterase activity provide a good estimate of the normal values in free-living individuals of the species Zonotrichia capensis, Molothrus bonariensis, Passer domesticus, Diuca diuca, Poospiza ornata, Saltator aurantiirostris, Gryseotyrannus aurantioatrocristatus, and Columbina picui, with interspecies differences. The median enzymatic levels ± standard error of the mean ranged from 546.31 ± 17.97 μmol min–1 L–1 in P. domesticus to 3439.90 ± 173.92 μmol min–1 L–1 in Tyrannus melancholicus. No significant differences were detected between different sexes or ages. Birds that migrate (which are also insectivores) showed higher levels of cholinesterase than residents (mainly granivores). It is recommended that in cases of bird poisoning, plasma cholinesterase activity can be used as a diagnostic tool only if pre-exposure levels obtained in the same species are available, and ideally evaluated in individuals from the same biogeographical region. Environ Toxicol Chem 2019;38:1692–1700. © 2019 SETAC
Abstract
Prosopis flexuosa, a shrubby tree characteristic of the central Monte Desert, Argentina, a passerine, and the plasma cholinesterase reaction. AChE = acetylcholinesterase.
GDNF‐ADSCs‐APG embedding enhances sciatic nerve regeneration after electrical injury in a rat model
Authors: Zheng Zhao , Liu Jiaqi 
Published: May 2019

AbstractThe pluripotency of adipose‐derived stem cells (ADSCs) makes them appropriate for tissue repair and wound healing. Owing to the repair properties of autologous platelet–rich gel (APG), which is based on easily accessible blood platelets, its clinical use has been increasingly recognized by physicians. The aim of this study was to investigate the effect of combined treatment with ADSCs and APG on sciatic nerve regeneration after electrical injury. To facilitate the differentiation of ADSCs, glial cell line–derived neurotrophic factor (GDNF) was overexpressed in ADSCs by lentivirus transfection. GDNF‐ADSCs were mingled with APG gradient concentrations, and in vitro, cell proliferation and differentiation were examined with 5‐ethynyl‐2′‐deoxyuridine staining and immunofluorescence. A rat model was established by exposing the sciatic nerve to an electrical current of 220 V for 3 seconds. Rat hind‐limb motor function and sciatic nerve regeneration were subsequently evaluated. Rat ADSCs were characterized by high expression of CD90 and CD105, with scant expression of CD34 and CD45. We found that GDNF protein expression in ADSCs was elevated after Lenti‐GDNF transfection. In GDNF‐ADSCs‐APG cultures, GDNF was increasingly produced while tissue growth factor‐β was reduced as incubation time was increased. ADSC proliferation was augmented and neuronal nuclei (NeuN) and glial fibrillary acidic protein (GFAP) expression were upregulated in GDNF‐ADSCs‐APG. In addition, limb motor function and nerve axon growth were improved after GDNF‐ADSCs‐APG treatment. In conclusion, our study demonstrates the combined effect of ADSCs and APG in peripheral nerve regeneration and may lead to treatments that benefit patients with electrical injuries.
Knockdown of FOXJ1 inhibits the proliferation, migration, invasion, and glycolysis in laryngeal squamous cell carcinoma cells
Authors: Liu Lifeng , Zhang Pengfei , Shao Yuan , Quan Fang , Li Huajing 
Published: May 2019

AbstractForkhead box (FOX) proteins are a large family of transcription factors that are involved in multiple biological processes. FOXJ1, a member of the FOX family, has been found to participate in tumorigenesis. However, the role of FOXJ1 in laryngeal squamous cell carcinoma (LSCC) is still unclear. The aim of the present study was to explore the potential roles of FOXJ1 in LSCC. Our results showed that FOXJ1 was overexpressed in LSCC tissues and cell lines. Then the small interfering RNA targeting FOXJ1 (FOXJ1‐siRNA) or control siRNA was transfected into TU‐177 and AMC‐HN‐8 cells to knockdown FOXJ1. Cell Counting Kit‐8 assay showed that knockdown of FOXJ1 inhibited the proliferation of LSCC cells. Transwell assay revealed that FOXJ1‐siRNA‐transfected cells showed significant reduction in cell migration and invasion compared to the cells transfected with control siRNA. FOXJ1 knockdown suppressed glycolysis in LSCC cells, which was illustrated by the reduced glucose consumption and lactate production. In addition, FOXJ1 knockdown inhibited the activation of the Wnt/β‐catenin pathway, and the LiCl treatment mitigated the inhibitory effects of FOXJ1‐siRNA on cell proliferation, migration, invasion, and glycolysis. These findings indicated that FOXJ1‐siRNA executed its functions via suppressing the activation of the Wnt/β‐catenin pathway.