bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nicastrin haploinsufficiency alters expression of type-I interferon-stimulated genes in two immortalized human cell lines
Authors: Cao Li , Morales-Heil David J. , Roberson Elisha D. O. 
Published: June 2018

A.AbstractBackgroundHidradenitis suppurativa (HS) is a chronic skin disease. The symptoms can be severe, and include intensely painful nodules and abscesses in apocrine-gland rich inverse skin, such as the buttocks, under the arms, and the groin. Autosomal dominant forms of HS exist, but are rare. Some of these kindred have heterozygous loss-of-function rare variants in the γ-secretase complex component nicastrin (NCSTN).ObjectivesWe wanted to know what effect NCSTN haploinsufficiency has on human keratinocytes to assess potential mechanisms for lesion development.MethodsWe knocked down nicastrin using an shRNA construct in both a keratinocyte cell line (HEK001) and an embryonic kidney cell line (HEK293). We assessed differential gene expression using RNA microarray. We also generated a NCSTN heterozygous deletion in the HEK293 line using CRISPR/Cas9 genome-editing and assessed NFKB activity in this line using a luciferase reporter.ResultsThe keratinocyte NCSTN knockdown cell line demonstrated significantly increased expression of genes related to the type-I interferon response pathway when compared to controls. Both HEK001 and HEK293 knockdowns demonstrated evidence for altered growth. We observed a small, but significant increase in NFKB signaling in response to TNF treatment a HEK293 line genome-edited for reduced NCSTN.ConclusionsOur data suggest a role for increased keratinocyte inflammatory responsiveness in familial HS. Confirming this phenotype, and characterizing additional effects in different cell types, will require study beyond cell lines in primary cells and tissues.
DARPins recognizing mTFP1 as novel reagents for in vitro and in vivo protein manipulations
Authors: Vigano M. Alessandra , Bieli Dimitri , Schaefer Jonas V. , Peter Jakob Roman , Matsuda Shinya , Maier Timm , Plückthun Andreas , Affolter Markus 
Published: June 2018

AbstractOver the last few years, protein-based affinity reagents have proven very helpful in cell and developmental biology. While many of these versatile small proteins can be expressed both in the intracellular and extracellular milieu in cultured cells and in living organisms, they can also be functionalized by fusing them to different protein domains in order to regulate or modulate their target proteins in diverse manners. For example, protein binders have been employed to degrade, trap, localize or enzymatically modify specific target proteins. Whereas binders to many endogenous proteins or small protein tags have been generated, also several affinity reagents against fluorescent proteins have been created and used to manipulate target proteins tagged with the corresponding fluorescent protein. Both of these approaches have resulted in improved methods for cell biological and developmental studies. While binders against GFP and mCherry have been previously isolated and validated, we now report the generation and utilization of designed ankyrin repeat proteins (DARPins) against the monomeric teal fluorescent protein 1 (mTFP1). Here we use the generated DARPins to delocalize Rab proteins to the nuclear compartment, in which they cannot fulfill their regular functions anymore. In the future, such manipulations might enable the production of acute loss-of-function phenotypes in different cell types or living organisms based on direct protein manipulation rather than on genetic loss-of-function analyses.Summary statementStructural characterization of two novel DARPins (designed ankyrin repeat proteins) recognizing the monomeric teal fluorescent protein 1 (mTFP1) and their functionalization for protein manipulation strategies in cultured cells and potentially in living organisms.
Sequence variability, constraint and selection in the CD163 gene in pigs
Authors: Johnsson Martin , Ros-Freixedes Roger , Gorjanc Gregor , Campbell Matt A. , Naswa Sudhir , Kelly Kimberly , Lightner Jonathon , Rounsley Steve , Hickey John M. 
Published: June 2018

AbstractBackgroundIn this paper, we investigate sequence variability, evolutionary constraint, and selection on the CD163 gene in pigs. The pig CD163 gene is required for infection by porcine reproductive and respiratory syndrome virus (PRRSV), a serious pathogen with major impact on pig production.ResultsWe used targeted pooled sequencing of the exons of CD163 to detect sequence variants in 35,000 pigs of diverse genetic backgrounds and search for potential knock-out variants. We then used whole genome sequence data from three pig lines to calculate a variant intolerance score, which measures the tolerance of genes to protein coding variation, a selection test on protein coding variation over evolutionary time, and haplotype diversity statistics to detect recent selective sweeps during breeding.ConclusionsWe performed a deep survey of sequence variation in the CD163 gene in domestic pigs. We found no potential knock-out variants. CD163 was moderately intolerant to variation, and showed evidence of positive selection in the lineage leading up to the pig, but no evidence of selective sweeps during breeding.
Are women really risk-averse? The lending behavior of women-owned banking cooperatives in India
Authors: Ghosh Saibal 
Published: June 2018

PurposeThe purpose of this paper is to examine the lending behavior of women-owned cooperatives (WoCs) by exploiting the natural experiment of the financial crisis, employing a novel data set of Indian cooperative banks during 2004–2013.Design/methodology/approachIn view of the longitudinal nature of the data, the authors employ panel data techniques for the purposes of analysis.FindingsThe findings indicate that WoC banks increased lending to both agriculture and small-scale industries, especially in high-income states. Further disaggregation reveals that the possible weaknesses in asset quality from lending to these sectors in low-income states could be driving the results.Originality/valueTo the best of our knowledge, this is one of the earliest studies for a leading emerging economy to empirically investigate the behavior of WoC banks and relatedly, how their behavior evolved during the financial crisis.
Chinese strategic thinking on competitive conflict: insights from Yin-Yang harmony cognition
Authors: Chin Tachia , Rowley Chris , Redding Gordon , Wang Shouyang 
Published: June 2018

Purpose
Grounded in Yijing, the wellspring of Chinese philosophies, this research aims to propose a novel interpretation of the indigenous Yin-Yang harmony cognitive framework, and to elaborate on how to use it as a meta-theorising tool to characterise the conflicting yet complementary dynamics of strategy, commonly seen as the prominent feature of Chinese strategic thinking.

Design/methodology/approach
Using the Yin-Yang harmony approach (i.e. Yin as the endogenous factors and Yang the exogenous factors), the authors first put forward eight paradoxical situations facing Chinese organisations as per the changing paradigm of Yijing. Then the authors use the thick description model as a roadmap to identify three evolving trajectories in Chinese higher education (HE) system. Finally, they raise four strategic propositions regarding how competing HE institutes handle the conflicting yet complementary dynamics in China.

Findings
Results show that the main strategic choices used by two different types of higher education institutes to cope with the current high-level uncertainty and competition could be described in terms of the two “Qian” and “Li” strategic situations, respectively. More details are discussed in the four propositions.

Research limitations/implications
This research brings potentially valuable implications for global regulators, policymakers, providers and other stakeholders through better understanding of HE-related issues, as well as certain distinct conceptual complexities in terms of developing strategies in China. It implies potentially significant differences in cognition between East and West, and illustrates what may be their workings.

Originality/value
This indigenous eight-dimensional paradigm demonstrates the conflicting yet complementary dynamic gestalt of organisational strategic choices that may only be realised in Chinese terms, and that cannot be elucidated by theories purely derived from Western experience. It thus can foster the transfer of understanding between the East and West and open a new chapter for future research.
The effect of human capital on regional financial conditions through cultural capital of employees
Authors: Nirwana Nirwana 
Published: June 2018

Purpose
This paper aims to discuss about whether human capital and cultural capital affect the financial condition of a region and whether cultural capital has a mediating effect (mediator) on the effect of human capital on the financial condition of a region. Moreover, this research also refines previous research by combining the simultaneous effect of the variables human capital and cultural capital on the financial condition of a region and examining the mediating effect of the variable cultural capital.

Design/methodology/approach
Research was conducted at the Regional Apparatus Work Unit (SKPD) in the Area of South Sulawesi Province with a total population of up to 630. The inferential statistical analysis using the method of variance-based structural equation modeling (SEM) is known as partial least squares SEM.

Findings
Human capital has positive influence on cultural capital. The higher human capital, primarily reflected in the work experience, will result in increasing the capital of culture in the working environment of local government in the province of South Sulawesi.

Originality/value
Originality of this paper is as follows: an inferential method used in this research is an SEM. The location of the research located in of the Regional Apparatus Work Unit (SKPD) in the Area of South Sulawesi Province, Indonesia, Indonesia which there has not been research by the methods and the same location.
The effect of social capital and knowledge sharing to the small medium enterprise’s performance and sustainability strategies
Authors: Meflinda Astuti , Mahyarni Mahyarni , Indrayani Henni , Wulandari Hesty 
Published: June 2018

Purpose
The development of small- and medium-sized enterprises (SMEs) in Indonesia is one of the national economic development priorities. SMEs have given benefits for society, especially in creating a fair income distribution and supporting economic growth. This paper aims to examine and analyze the impact of social capital and knowledge sharing on the sustainability strategy and performance of SME and to formulate policies about SMEs in the future.

Design/methodology/approach
This research was carried out in Riau Province, which is the closest province to Malaysia and Singapore. The authors collected data from 56 SMEs in trading and craft industries by using the purposive sampling method. The data were analyzed using partial least square technique.

Findings
The result of data analysis shows that social capital and knowledge sharing significantly affect the sustainability strategy of SMEs. Furthermore, sustainability strategy and knowledge sharing affects SME performance significantly, whereas social capital does not affect its performance.

Originality/value
A participatory approach (partial least square) was used, the location of the research was Riau Province, Indonesia and the participants in this study are the perpetrators of the micro-, small- and medium-sized enterprises. This method and location have not been considered in earlier studies.
Introducing data analytics to the robotic drilling process
Authors: Al Khawli Toufik , Bendemra Hamza , Anwar Muddasar , Swart Dewald , Dias Jorge 
Published: June 2018

Purpose
This paper presents a method for extracting the geometric primitives of a circle in a three-dimensional space from a discrete point cloud data set obtained by a laser stripe sensor. This paper aims to first establish a reference frame for the robotic drilling process by detecting the position and orientation of a reference hole on structural parts in a pre-drilling step, and second, to perform quality inspection of the hole in a post-drilling step.

Design/methodology/approach
The method is divided into the following steps: a plane is initially fitted on the data by evaluating the principle component analysis using singular value decomposition; the data points or measurements are then rotated around an arbitrary axis using the Rodrigues’ rotation formula such that the normal direction of the estimated plane and the z-axis direction is parallel; the Delaunay triangulation is constructed on the point cloud and the confidence interval is estimated for segmenting the data set located at the circular boundary; and finally, a circular profile is fitted on the extracted set and transformed back to the original position.

Findings
The geometric estimation of the circle in three-dimensional space constitutes of the position of the center, the diameter and the orientation, which is represented by the normal vector of the plane that the circle lives in. The method is applied on both simulated data set with the addition of several noise levels and experimental data sets. The main purpose of both the tests is to quantify the accuracy of the estimated diameter. The results show good accuracy (mean relative error < 1 per cent) and high robustness to noise.

Research limitations/implications
The proposed method is applied here to estimate the geometric primitives of only one circle (the reference hole). If multiple circles are needed, an addition clustering procedure is required to cluster the segmented data into multiple data sets. Each data set represents a circle. Also, the method does not operate efficiently on a sparse data sets. Dense data are required to cover the hole (at least ten scans to cover the hole diameter).

Practical implications
Researchers and practitioners can integrate this method with several robotic manufacturing applications where high accuracy is required. The extracted position and orientation of the hole are used to minimize the positioning and alignment errors between the mounted tool tip and the workpiece.

Originality/value
The method introduces data analytics for estimating the geometric primitives in the robotic drilling application. The main advantage of the proposed method is to register the top surface of the workpiece with respect to robot base frame with a high accuracy. An accurate workpiece registration is extremely necessary in the lateral direction (identifying where to drill), as well as in the vertical direction (identifying how far to drill).
The effect of additional egg supplementation on vitamin and mineral fortification program on growth, cognitive development and hemoglobin in Indonesian underweight and stunting children
Authors: Sudargo Toto , Muhammad Harry Freitag Luglio , Kandarina Istiti , Putri Nurul , Irianto Sugeng Eko , Pranoto Yosephin Anandati , Paramastri Rathi 
Published: June 2018

Purpose
Stunting and being underweight in children are major nutritional problems especially in developing countries. The purpose of this study is to evaluate the effect of egg supplementation on a vitamin and mineral fortification program for growth, cognitive development and hemoglobin in underweight and stunted children.

Design/methodology/approach
This was an experimental study using a crossover design conducted in Yogyakarta province, Indonesia. A total of 39 subjects were randomly selected from two community health centers and provided with two types of intervention: vitamin and mineral fortification sprinkle (Taburia) and Taburia with egg supplementation (Taburia PLUS). Each intervention was conducted for three months with one-month of washout period in between interventions.

Findings
There were no changes in weight-for-age Z-score in Taburia and Taburia PLUS (all p > 0.05). The height-for-age Z-score was increased in Taburia PLUS (p = 0.022) but not in Taburia (p > 0.05). Hemoglobin level was significantly increased in Taburia (p = 0.039) but not in Taburia PLUS (p > 0.05). There were no significant changes in visual motoric score in Taburia, but there were slight increases in Taburia PLUS (all p > 0.05).

Originality/value
The authors concluded that egg supplementation combined with multivitamins and minerals fortification program, Taburia PLUS, is beneficial to improve linear growth but not hemoglobin in stunted and underweight children.
Applying spatial literacy to transform library space: a selected literature review
Authors: Gray Juliet Kerico , Burel Melissa , Graser Marlee , Gallacci Karen 
Published: June 2018

Purpose
The purpose of this paper is to review a selection of articles and books that highlight aspects of spatial theory and literacy from various disciplinary perspectives, along with a review of library space studies.

Design/methodology/approach
This study reviews library literature that uses spatial literacy and its related tools. The authors searched in two databases: Library, Information Science & Technology Abstracts, and SCOPUS. The paper records were analyzed to find primary research studies, published between 2010 and 2017, which study patron use of library space using various single and hybrid methodologies.

Findings
The findings of the literature reveal that of the 26 studies reviewed, 23 have a descriptive research question and three have a relational research question. Based on the analysis of the research methodologies used, there is more that can be done in support of a librarian’s research efforts as well as the arenas in which research is conducted.

Practical implications
These findings highlight ways in which library and information science researchers and those who educate them can broaden knowledge within the profession regarding spatial theory, literacy and applicable research methodologies for studying library space.

Originality/value
Current and best practices for designing library space studies that use spatial literacy to collect and analyze data are identified along with a discussion of future directions for researchers to better assess space and communicate the value of physical space in libraries.
Self‐reported environmental health risks of nurses working in hospital surgical units
Authors: Azizoğlu F. , Köse A. , Gül H. 
Published: June 2018

AimThis study investigated the occupational health risk factors among nurses who work in public hospital surgical units.BackgroundNursing has a significant place in healthcare systems around the world. Surgical units are environments with certain risks, especially because of the possibility of exposure to various chemical, biologic or physical hazards.MethodsThis study was conducted with 229 nurses who were working in the 11 surgery units of a big university hospital. In this cross‐sectional study, a personal information form and an occupational risk factors scale were administered to respondents. We performed factor and reliability analyses for the scale; the overall reliability of the 41 items was α = 0.924, and the factor analysis found the scale was feasible.ResultsBiologic and psychological risk factor levels were found to be high. Physical, chemical, ergonomic and radiation risk factor levels were moderate. The general occupational risk factor score was moderate. Nurses working night duty were confronted with physical and psychological risk factors at a higher rate compared with those working in the daytime.ConclusionReported occupational health problems by nurses were correlated with the descriptive properties of the nurses including age, sex, marital status, education level, working hours, mode of working and status of occupational health and safety training.Implications for nursingNurses experience different occupational risks. If these risks are identified, healthier working environments can be provided to the nurses by taking necessary precautions.Implications for social policyThe health care provided by nurses who work in a healthy environment would be more efficient and of better quality, which will result in better economic and social outcomes for individual and communities.
Periodontal health
Authors: Lang Niklaus P. , Bartold P. Mark 
Published: June 2018

AbstractObjectivesTo date there is a paucity of documentation regarding definitions of periodontal health. This review considers the histological and clinical determinants of periodontal health for both intact and reduced periodontium and seeks to propose appropriate definitions according to treatment outcomes.ImportanceDefining periodontal health is can serve as a vital common reference point for assessing disease and determining meaningful treatment outcomes.FindingsThe multifactorial nature of periodontitis is accepted, and it is recognized that restoration of periodontal health will be defined by an individual's response to treatment, taking into account allostatic conditions.ConclusionsIt is proposed that there are 4 levels of periodontal health, depending on the state of the periodontium (structurally and clinically sound or reduced) and the relative treatment outcomes: (1) pristine periodontal health, with a structurally sound and uninflamed periodontium; (2) well‐maintained clinical periodontal health, with a structurally and clinically sound (intact) periodontium; (3) periodontal disease stability, with a reduced periodontium, and (4) periodontal disease remission/control, with a reduced periodontium.
Classification and diagnosis of aggressive periodontitis
Authors: Fine Daniel H. , Patil Amey G. , Loos Bruno G. 
Published: June 2018

AbstractObjectiveSince the initial description of aggressive periodontitis (AgP) in the early 1900s, classification of this disease has been in flux. The goal of this manuscript is to review the existing literature and to revisit definitions and diagnostic criteria for AgP.Study analysisAn extensive literature search was performed that included databases from PubMed, Medline, Cochrane, Scopus and Web of Science. Of 4930 articles reviewed, 4737 were eliminated. Criteria for elimination included; age > 30 years old, abstracts, review articles, absence of controls, fewer than; a) 200 subjects for genetic studies, and b) 20 subjects for other studies. Studies satisfying the entrance criteria were included in tables developed for AgP (localized and generalized), in areas related to epidemiology, microbial, host and genetic analyses. The highest rank was given to studies that were; a) case controlled or cohort, b) assessed at more than one time‐point, c) assessed for more than one factor (microbial or host), and at multiple sites.ResultsEpidemiologic studies provided insight into ethnic and societal factors affecting AgP. DNA analysis of microbes showed some consistency but significant variability. Host factor analysis was less consistent. Many genetic studies were conducted but few had either sufficient power or looked at multiple genes in AgP.ConclusionsConflicting data resulted for several reasons; 1) the classification was too broad, 2) the disease (AgP) was not studied from its inception, at differing time points (temporal), and at different locations (topographic). New technologic advances coupled with a more delimiting definition of disease will allow for genetic, host and microbial factor analyses in an unbiased manner. As such we predict that progress can be made in identifying a robust group of genetic, host, and microbial risk‐markers associated with periodontal disease that can improve diagnostic capability in disease associated with juveniles, adolescents, and post‐adolescent individuals.