bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluating the Need for Pediatric Procedural Sedation Training in Pediatric Critical Care Medicine Fellowship*
Authors: Hooper Michael C. , Kamat Pradip P. , Couloures Kevin G. 
Published: November 2018

Objectives:
Pediatric procedural sedation has been increasingly performed by pediatric intensivists over the past decade. Pediatric Critical Care Medicine fellowship guidelines do not specify how fellows obtain proficiency in pediatric procedural sedation. We sought to survey the state of pediatric procedural sedation training during fellowship and whether fellows thought it was sufficient.

Design:
A 21-question survey gathered data on pediatric procedural sedation training provided to Pediatric Critical Care Medicine fellows. Surveys were sent to fellowship directors with instructions to distribute to second- and third-year fellows or recent graduates. Over 2 months, up to three e-mail reminders were sent to fellowship directors whose program had not completed at least one survey.

Subjects:
Senior fellows and graduates of 65 active Accreditation Council for Graduate Medical Education Pediatric Critical Care Medicine fellowship programs.

Interventions:
None.

Measurements and Main Results:
Sixty-five percent of fellowship programs (42/65) returned at least one response. Ninety senior fellows and 27 recent graduates responded. Of respondents, 38% received pediatric procedural sedation training during the fellowship, and 32% reported mandatory training. Nine percent of programs used simulation. Although 61% who received training felt adequately prepared to perform pediatric procedural sedation, 25% needed additional preceptorship to sedate independently. Nearly one third (31%) reported that completion of a predetermined number of cases was required to sedate independently. Forty-eight percent reported a minimum number of cases was required for hospital credentialing. Nearly 45% were allowed to perform pediatric procedural sedation off the unit after receiving credentials. When asked if inadequate pediatric procedural sedation training would be a deterrent to applying for a position that included pediatric procedural sedation, 8.6% replied yes, 52.6% replied no, and 38.8% replied they were unsure.

Conclusions:
Pediatric procedural sedation lacks a clearly defined training pathway. Most fellows find pediatric procedural sedation a valuable skill set. We propose that all Pediatric Critical Care Medicine fellows receive training that includes pediatric procedural sedation critical incident simulation and cases performed outside the PICU to establish proficiency.
Serum Interleukin 35 Levels in Systemic Sclerosis and Relationship With Clinical Features
Authors: Yayla Müçteba Enes , Torgutalp Murat , Okatan İlyas Ercan , Yurteri Emine Uslu , Küçükşahin Orhan , Dinçer Ayşe Bahar Keleşoğlu , Gülöksüz Emine Gözde Aydemir , Sezer Serdar , Us Ebru , Turgay Tahsin Murat , Kinikli Gülay , Ateş Aşkin 
Published: November 2018

Background/Objective
Interleukin (IL) 35 is a member of the IL-12 family. Studies show that IL-35 is an important anti-inflammatory cytokine and suppresses effector T-cell activity. In this study, we aimed to evaluate serum IL-35 levels in systemic sclerosis (SSc) patients and its potential relation with clinical findings.

Methods
We conducted a cross-sectional analysis of 70 SSc patients and 29 healthy volunteers in a single center in 5 months' period. Extension of skin fibrosis was evaluated by using modified Rodnan skin score. Disease severity was assessed by Medsger disease severity scores. Serum IL-35 was measured using a commercial enzyme-linked immunosorbent assay (ELISA) kit (Cloud-Clone Corp, Wuhan, China). The relationship between IL-35 levels and clinical and laboratory parameters was investigated. Mann-Whitney U test was used to compare parameters among the groups. Correlation was tested by Spearsman correlation coefficient.

Results
Serum IL-35 levels was significantly higher in SSc patients (8.69 [interquartile range, 29.33] pg/mL) than in healthy controls (7.11 [interquartile range 7.53] pg/mL; p < 0.001). There was no significant relationship between serum IL-35 levels and organ involvement. There was a negative correlation between serum IL-35 levels and Medsger disease severity score (Rho, −0.333; p = 0.006), modified Rodnan skin score (Rho, −0.307; p = 0.010), and C-reactive protein (Rho, −0.294; p = 0.015). There was no relationship between IL-35 and disease duration and erythrocyte sedimentation rate.

Conclusions
Our study revealed that IL-35 levels were higher in SSc patients, and in contrast to previous studies, it was the first study that showed that IL-35 levels did not increase in SSc patients with pulmonary fibrosis.
CD36himonocytes play immunoregulatory roles in human umbilical cord blood
Authors: Lee Jessica G. , Jaeger Kathleen E. , Seki Yoichi , Nelson Alexander J. , Vuchkovska Alexandra , Nishimura Michael I. , White Paula , Knight Katherine L. , Iwashima Makio 
Published: November 2018

AbstractThe fetal and neonatal immune systems are uniquely poised to generate tolerance to self, maternal, and environmental antigens encountered in the womb and shortly after birth. The tolerogenic nature of fetal and neonatal immunity is a rising health concern with the spread of vertically transmitted viruses, such as the Zika virus. A variety of mechanisms contribute to fetal and neonatal tolerance, including a propensity to generate Foxp3+regulatory T cells (Tregs). Here, we demonstrate that a subset of CD14+monocytes expressing the scavenger molecule, CD36, is able to generate CD4+and CD8+T cells that express Foxp3 from umbilical cord blood (UCB). Monocyte-induced Foxp3+T cells have potent suppressive functions on T cell proliferation and maintain Foxp3 expression over six weeksin vitro. Importantly, UCB-derived Foxp3+T cells are distinguishable from adult peripheral blood (APB) CD4+CD25+Tregs by surface antigen expression. While UCB-derived Foxp3+T cells express prototypic Treg-associated surface antigens, such as CD25 and glucocorticoid-induced tumor necrosis factor-related receptor (GITR), only UCB-derived Foxp3+T cells express CD26. In addition, most UCB-derived CD8+Foxp3+T cells express CD31. Mechanistically, both APB and UCB-derived monocytes support the development of Foxp3+T cells from naïve T cells, but APB naïve T cells are less efficient in expressing Foxp3 than UCB naïve T cells. These data suggest that antigen presentation by CD36himonocytes in the fetus leads to the development of a group of T cells that share some but not all phenotypes of adult thymus-derived Tregs.
You’d better decide first: overt and covert decisions improve metacognitive accuracy
Authors: Siedlecka Marta , Skóra Zuzanna , Paulewicz Borysław , Wierzchoń Michał 
Published: November 2018

AbstractHow can we assess the accuracy of our decisions? Recent theoretical and empirical work suggest that confidence in one’s decision is influenced by the characteristics of motor response in a preceding decisional task. In this paper we present experiment designed to test whether decision itself can also inform confidence and therefore increase its accuracy. We tested 143 participants who solved an anagram-solving task in one of 3 conditions: participants either rated their confidence immediately after responding to the anagram task (overt decision), rated their confidence immediately after making a decision but without overt response (covered decision), or rated their confidence before both deciding and responding. The results showed significant relationship between decision accuracy and confidence level in each condition, however this relation was stronger when confidence rating followed decision, either covert or overt. We argue that completing a decisionmaking process increases metacognitive accuracy.
The Drosophila pioneer factor Zelda modulates the nuclear microenvironment of a Dorsal target enhancer to potentiate transcriptional output
Authors: Yamada Shigehiro , Whitney Peter H. , Huang Shao-Kuei , Eck Elizabeth C. , Garcia Hernan G. , Rushlow Christine A. 
Published: November 2018

SummaryConnecting the developmental patterning of tissues to the mechanistic control of RNA polymerase II remains a long term goal of developmental biology. Many key elements have been identified in the establishment of spatial-temporal control of transcription in the early Drosophila embryo, a model system for transcriptional regulation. The dorsal/ventral axis of the Drosophila embryo is determined by the graded distribution of Dorsal (DL), a homologue of the NF-κB family of transcriptional activators found in humans [1,2]. A second maternally deposited factor, Zelda (ZLD), is uniformly distributed in the embryo and is thought to act as a pioneer factor, increasing enhancer accessibility for transcription factors such as DL [3–9]. Here we utilized the MS2 live imaging system to evaluate the expression of the DL target gene short gastrulation (sog) to better understand how a pioneer factor affects the kinetic parameters of transcription. Our experiments indicate that ZLD modifies probability of activation, the timing of this activation, and the rate at which transcription occurs. Our results further show that this effective rate increase is due to an increased accumulation of DL at the site of transcription, suggesting that transcription factor “hubs” induced by ZLD [10] functionally regulate transcription.
Reward Modulates Local Field Potentials, Spiking Activity and Spike-Field Coherence in the Primary Motor Cortex
Authors: An Junmo , Yadav Taruna , Hessburg John P. , Francis Joseph T. 
Published: November 2018

ABSTRACTReward modulation of the primary motor cortex (M1) could be exploited in developing an autonomously updating brain-machine interface (BMI) based on a reinforcement learning architecture. In order to understand the multifaceted effects of reward on M1 activity, we investigated how neural spiking, oscillatory activities and their functional interactions are modulated by conditioned stimuli related reward expectation. To do so, local field potentials (LFPs) and singleunit/multi-unit activities were recorded simultaneously and bilaterally from M1 cortices while five non-human primates performed cued center-out reaching or grip force tasks either manually using their right arm/hand or observed passively. We found that reward expectation influenced the strength of alpha (8-14 Hz) power, alpha-gamma comodulation, alpha spike-field coherence, and firing rates in general in M1. Furthermore, we found that an increase in alpha-band power was correlated with a decrease in neural spiking activity, that firing rates were highest at the trough of the alpha-band cycle and lowest at the peak of its cycle. These findings imply that alpha oscillations modulated by reward expectation have an influence on spike firing rate and spike timing during both reaching and grasping tasks in M1. These LFP, spike, and spike-field interactions could be used to follow the M1 neural state in order to enhance BMI decoding (An et al., 2018; Zhao et al., 2018).Significance StatementKnowing the subjective value of performed or observed actions is valuable feedback that can be used to improve the performance of an autonomously updating brain-machine interface (BMI). Reward-related information in the primary motor cortex (M1) may be crucial for more stable and robust BMI decoding (Zhao et al., 2018). Here, we present how expectation of reward during motor tasks, or simple observation, is represented by increased spike firing rates in conjunction with decreased alpha (8-14 Hz) oscillatory power, alpha-gamma comodulation, and alpha spike-field coherence, as compared to non-rewarding trials. Moreover, a phasic relation between alpha oscillations and firing rates was observed where firing rates were found to be lowest and highest at the peak and trough of alpha oscillations, respectively.
Title:In vivophosphatidylserine variations steer Rho GTPase signaling in a cell-context dependent manner
Authors: Platre Matthieu Pierre , Bayle Vincent , Armengot Laia , Bareille Joseph , Marques-Bueno Maria Mar , Creff Audrey , Maneta-Peyret Lilly , Fiche Jean-Bernard , Nolmann Marcelo , Miège Christine , Moreau Patrick , Martinière Alexandre , Jaillais Yvon 
Published: November 2018

AbstractRho GTPases are master regulators of cell signaling, but how they are regulated depending on the cellular context is unclear. Here, we show that the phospholipid phosphatidylserine acts as a developmentally-controlled lipid rheostat that tunes Rho GTPase signaling in Arabidopsis. Live super-resolution single molecule imaging revealed that RHO-OF-PLANT6 (ROP6) is stabilized by phosphatidylserine into plasma membrane (PM) nanodomains, which is required for auxin signaling. Furthermore, we uncovered that the PM phosphatidylserine content varies during plant root development and that the level of phosphatidylserine modulates the quantity of ROP6 nanoclusters induced by auxin and hence downstream signaling, including regulation of endocytosis and gravitropism. Our work reveals that variations in phosphatidylserine levels are a physiological process that may be leveraged to regulate small GTPase signaling during development.One Sentence SummaryPhosphatidylserine acts as a developmentally-controlled lipid rheostat that regulates cellular auxin sensitivity and plant development.
In Vivo Two-Photon Imaging of Neuronal and Vascular Responses in Mice Chronically Exposed to Ethanol
Authors: O’Herron Phillip , Summers Phillip M. , Shih Andy Y. , Kara Prakash , Woodward John J. 
Published: November 2018

AbstractThe effects of ethanol on brain function have been extensively studied using a variety of in vitro and in vivo techniques. For example, electrophysiological studies using brain slices from rodents and nonhuman primates have demonstrated that acute and chronic exposure to ethanol alters the intrinsic excitability and synaptic signaling of neurons within cortical and sub-cortical areas of the brain. In humans, neuroimaging studies reveal alterations in measures of brain activation and connectivity in subjects with alcohol use disorders. While complementary, these methods are inherently limited due to issues related to either disruption of normal sensory input (in vitro slice studies) or resolution (whole brain imaging). In the present study, we used 2-photon laser scanning microscopy in intact animals to assess the impact of chronic ethanol exposure on sensory evoked neuronal and vascular responses. Adult male C57BL/6J mice were exposed to 4 weekly cycles of chronic intermittent ethanol (CIE) exposure while control mice were exposed to air. After withdrawal (≤ 72 hr), a cranial window was placed over the primary visual cortex (V1) and sensory evoked responses were monitored using the calcium indicator OGB-1. CIE exposure produced small but significant changes in response amplitude (decrease) and orientation selectivity of V1 neurons (increase). While arteriole diameter did not differ between control and CIE mice under baseline conditions, sensory-evoked dilation was enhanced in vessels from CIE exposed mice as compared to controls. This was accompanied by a reduced latency in response to stimulation. In separate experiments, pial arteriole diameter was measured in the barrel cortex of control and CIE exposed mice. Baseline diameter of barrel cortex arterioles was similar between control and CIE exposed mice but unlike vessels in V1, sensory-evoked dilation of barrel cortex arterioles was similar between the two groups. Together the results of these studies suggest that chronic exposure to alcohol induces changes in neurovascular coupling that are region dependent.
Effects of Biogas Slurry on Fruit Economic Traits and Soil Nutrients of Camellia oleifera Abel
Authors: You Lu , Yu Shuqin , Liu Huiyun , Wang Chutian , Zhou Zengliang , Zhang Ling , Hu Dongnan 
Published: November 2018

AbstractSoil nutrients play a principal role in Camellia oleifera Abel (oil-seed camellia) production. Camellia oleifera absorbs nutrients from surrounding soils and its production is highly influenced by nutrients or fertilization. In this study, we investigated the effects of biogas slurry applications on soil nutrients and economic traits of C. oleifera fruits. Five different amounts of fertilizing biogas slurry (0, 10, 20, 30, or 40 kg/plant/year from three applications per year) were applied to C. oleifera plants in 2015 and 2016. Rhizosphere soil nutrients and C. oleifera fruit economic traits (yield, seed rate, and oil yield)were measured. Fertilization with biogas slurryincreasedsoil organic matter, available nitrogen (N), phosphorus (P), and potassium (K) in both 2015 and 2016. Increases in soil available N, P, and Kwere largest at the highest slurry application rate and second largest at the second highest application rate. Fruit economic traits were maximized at the two highest application rates. Oil yield was correlated withsoil available P in 2015 and 2016, and soil organic matter in 2015. Fertilization with biogas slurry decreased saturated fatty acid content in fruit but had no effect on unsaturated fatty acid content. In conclusion, fertilization with biogas slurry increases rhizosphere soil nutrients and fruit economic traits of C. oleifera with the rates of at least 30 kg/plant/year having the most positive effects.
The impact of modern jazz dance on the electrical brain activity
Authors: Wind Johanna , Schöllhorn Wolfgang 
Published: November 2018

AbstractDance as one of the earliest cultural assets of mankind is practised in different cultures, mostly for wellbeing or for treating psycho-physiological disorders like Parkinson, depression, autism. However, the underlying neurophysiological mechanisms are still unclear and only few studies address the effects of particular dance styles. For a first impression, we were interested in the effects of modern jazz dance (MJD) on the brain activation that would contribute to the understanding of these mechanisms. 11 female subjects rehearsed a MJD choreography for three weeks (1h per week) and passed electroencephalographic (EEG) measurements in a crossover-design thereafter. The objectives were to establish the differences between dancing physically and participating just mentally with or without music. Therefore, each subject realized the four following test conditions: dancing physically to and without music, dancing mentally to and without music. Each of the conditions were performed for 15 minutes. Before and after each condition, the EEG activities were recorded under resting conditions (2 min. eyes-open, 2 min. eyes-closed) followed by a subsequent wash-out phase of 10 minutes.The results of the study revealed no time effects for the mental dancing conditions, either to or without music. An increased electrical brain activation was followed by the physical dancing conditions with and without music for the theta, alpha-1, alpha-2, beta and gamma frequency band across the entire scalp. Especially the higher frequencies (alpha-2, beta, gamma) showed increased brain activation across all brain areas. Higher brain activities for the physical dancing conditions were identified in comparison to the mental dancing condition. No statistically significant differences could be found as to dancing to or without music. Our findings demonstrate evidence for the immediate influence of modern jazz dance and its sweeping effects on all brain areas for all measured frequency bands, when dancing physically. In comparison, dancing just mentally does not result in similar effects.
Three autoregressive-neural network hybrid models for energy efficiency estimation of induction motors
Authors: Sertsöz Mine , Fidan Mehmet , Kurban Mehmet 
Published: November 2018

Purpose
Improvements on the energy efficiency of the induction motors bear on not only these motors but also on the whole industry as a result of preference of these types of motors. In recent projects, energy efficiency of the induction motors is approaching to 90 per cent. The first necessary condition of the efficiency improvements is an accurate estimation of energy efficiency. This study aims to estimate the energy efficiency of induction motors by using three innovative estimation methods.

Design/methodology/approach
Data for 307 motors were taken from three different companies and their torque, power, power factor and speed data were used. Three hybrid models were created by estimating the error of three autoregressive (AR)-based efficiency estimation models with the back-propagation artificial neural network (ANN) structure. In these proposed hybrid models, the AR models were supported with artificial neural networks to obtain a minimum estimation error. These three hybrid models were called as AR1-ANN, AR4-ANN and residual-ANN.

Findings
Without hybridization of AR models by back-propagation ANNs, the best estimation result was obtained by residual model. On the other hand, for the proposed hybrid models, the best estimation was obtained by AR1-ANN, followed by AR4-ANN and finally the residual-ANN according to ME values.

Practical implications
Proposed AR-ANN hybrid models relieve of longtime experiments for the energy efficiency measurement of induction motors. Furthermore, these AR-ANN models give more accurate results than the available methods in the literature. Engineering value of this research is three different issues in finding energy efficiency. The first one is minimizing of the test cost, the second one is no requirement the test equipment and the third one is not interrupting the motor. Every company that needs motors can use these estimation methods due to the advantages.

Originality/value
Novel three AR-ANN hybrid models for energy efficiency estimation were studied. These novel methods give better response than the other methods which were used for estimation of induction motors in the literature.
The effects of environmental factors on B2B e-services purchase: perceived risk and convenience as mediators
Authors: Matos Celso Augusto de , Krielow Anderson 
Published: November 2018

Purpose
Based on the stimulus-organism-response (SOR) framework, this paper aims to analyze the influence of environmental factors (e.g. competitive pressure) on the firm’s evaluations (i.e. perceived risk and convenience) and response (intention to purchase e-services).

Design/methodology/approach
A model is tested with data from a survey with 430 micro- and small-sized enterprises (MSEs) in an emerging country. The following constructs were measured: external factors, data security, lack of knowledge, perceived risk, convenience and purchase intention. Company size, internet use and previous experience were control variables.

Findings
MSEs’ intention to purchase e-services is strongly influenced by convenience, which in turn is more affected by external factors. Perceived risk is mainly affected by lack of knowledge and data security. Overall, the model supports the mediating role of perceived risk and convenience in the relationship between Stimuli factors (external factors, data security and lack of knowledge) on response (purchase intentions). For instance, data security influences purchase intention only through the mediation of perceived risk and convenience.

Practical implications
Firms interested in providing e-services for MSEs should improve the perceived convenience of e-commerce. At the same time, they can also reduce the lack of knowledge and increase data security to reduce the perceived risk of the decision-maker.

Originality/value
This paper demonstrates mediating effects of perceived risk and convenience, considering a SOR framework, as well as the analysis of business-to-business e-services in an understudied context, i.e. MSEs in an emerging country.