bluuebunny/crossref_metadata_2025_split huggingface dataset:
Individual Radiosensitivity Assessment of the Families of Ataxia-Telangiectasia Patients by G2-Checkpoint Abrogation
Authors: Aghamohammadi Asghar , Akrami Seyed M. , Yaghmaie Marjan , Rezaei Nima , Azizi Gholamreza , Yaseri Mehdi , Nosrati Hassan , Zaki-Dizaji Majid 
Published: March 2019

Objectives: Ataxia-telangiectasia (A-T) is an autosomal recessive multisystem disorder characterised by cerebellar degeneration, telangiectasia, radiation sensitivity, immunodeficiency, oxidative stress and cancer susceptibility. Epidemiological research has shown that carriers of the heterozygous ataxia-telangiectasia mutated (ATM) gene mutation are radiosensitive to ionising irradiation and have a higher risk of cancers, type 2 diabetes and atherosclerosis. However, there is currently no fast and reliable laboratory-based method to detect heterozygous ATM carriers for family screening and planning purposes. This study therefore aimed to evaluate the ability of a modified G2-assay to identify heterozygous ATM carriers in the families of A-T patients. Methods: This study took place at the Tehran University of Medical Sciences, Tehran, Iran, between February and December 2017 and included 16 A-T patients, their parents (obligate heterozygotes) and 30 healthy controls. All of the subjects underwent individual radiosensitivity (IRS) assessment using a modified caffeine-treated G2-assay with G2-checkpoint abrogation. Results: The mean IRS of the obligate ATM heterozygotes was significantly higher than the healthy controls (55.13% ± 5.84% versus 39.03% ± 6.95%; P <0.001), but significantly lower than the A-T patients (55.13% ± 5.84% versus 87.39% ± 8.29%; P = 0.001). A receiver operating characteristic (ROC) curve analysis of the G2-assay values indicated high sensitivity and specificity, with an area under the ROC curve of 0.97 (95% confidence interval: 0.95–1.00). Conclusion: The modified G2-assay demonstrated adequate precision and relatively high sensitivity and specificity in detecting heterozygous ATM carriers.Keywords: Ataxia-Telangiectasia; Chromosome Breakage; Genetic Carrier Screening; Heterozygote; Radiation Sensitivity; Sensitivity and Specificity.
Food Allergen Sensitisation Patterns in Omani Patients with Allergic Manifestations
Authors: Al-Tamemi Salem , Naseem Shafiq-Ur-Rehman , Tufail-Alrahman Munira , Al-Kindi Mahmood , Alshekaili Jalila 
Published: March 2019

Objectives: This study aimed to evaluate the relationship between food allergen sensitisation patterns and allergic manifestations in Omani patients and highlight the importance of specific immunoglobulin E (IgE) testing. Methods: This retrospective study included all patients referred due to allergic manifestations to the Sultan Qaboos University Hospital (SQUH), Muscat, Oman, from November 2012 to November 2016. Specific IgE blood testing was performed to determine sensitisation to common foods known to cause allergic reactions. Results: A total of 164 patients were referred to SQUH over the study period, with 35.4% presenting with one allergic manifestation, 48.8% with 2–3 and 15.9% presenting with more than three manifestations. There was a familyhistory of allergies in 70.7% of patients. Eosinophil counts and total and specific IgE levels were elevated in 18.9%, 54.9% and 73.2% of patients, respectively. Patients demonstrated sensitisation to cow milk (47.6%), wheat (41.5%),chicken eggs (34.8%), mixed tree nuts (34.1%), lentils (33.5%), peanuts (32.9%), soy (32.3%), shrimp (23.2%) and fish (15.2%). Overall, 19.5% were sensitised to a single allergen, 14% were sensitised to 2–3 and 39.6% were sensitised to more than three allergens. Almost one-third (29.3%) of patients suffered from food-induced anaphylaxis, of which 85.4% were prescribed self-injectable adrenaline. Conclusion: To the best of the authors’ knowledge, this study is the first to describe food allergen sensitisation patterns among Omani patients with allergic manifestations. In conjunction with clinical symptoms, the correct interpretation of specific IgE levels is important to diagnose food allergies and make safe decisions about reintroducing foods.Keywords: Hypersensitivity; Food Allergies; Anaphylaxis; Urticaria; Atopic Dermatitis; Asthma; Immunoglobulin E; Oman.
The protective effect of vitamin E on rats’ ovarian follicles following an administration of diazinon: An experimental study
Authors: Sargazi Zinat , Nikravesh Mohammad Reza , Jalali Mehdi , Sadeghnia Hamid Reza , Anbarkeh Fatemeh Rahimi 
Published: March 2019

Background: Diazinon (DZN) is an organophosphate insecticide that has been widely utilized in agriculture all over the world and caused many negative effects on different species such as plants and animal species, especially on a human.
Objective: The aim of the present study was to evaluate the protective effect of vitamin E on rats’ ovarian follicles following an administration of diazinon.
Materials and Methods: A total of 30 adult female Wistar rats were divided into five groups: a control group (without any intervention), sham group (received only pure olive oil, as solvent), experimental group I (DZN+olive oil, 60 mg/kg), experimental group II (vitamin E, 200 mg/kg), and experimental group III (DZN: 60 mg/kg+vitamin E: 200 mg/kg). All drugs were injected intraperitoneally, except vitamin E which was administrated by gavage. The animals were sacrificed after two weeks and the left ovary was used to measure proliferation of ovarian follicles. Tissues were analyzed by the PCNA technique and viewed with an optical microscope for evaluating cell proliferation.
Results: The result of the present study revealed that the number of proliferative cells in the experimental group I decreased significantly in contrast to the control group in secondary and Graffian follicles (p< 0.001). The administration of vitamin E plus DZN significantly increased proliferative cells compared to the DZN group (p< 0.001). Primordial follicles showed that all study groups were lacking PCNA positive cells, which means no expression of PCNA in these follicles. The results of this study showed that primary follicles in all study groups had a few and scattered PCNA positive cells with no significant difference between the groups (p> 0.05).
Conclusion: Results showed that DZN reduced proliferation in secondary and Graffian follicles and vitamin E increased it. The results of this study suggested that vitamin E by its antioxidant activity was able to improve the DZN-induced ovarian toxicity.
Key words: Diazinon, Proliferation, Ovary, Vitamin E, Rat.
Effect of antioxidant therapy on the sperm DNA integrity improvement; a longitudinal cohort study
Authors: Salehi Peyman , Shahrokhi Seyedeh Zahra , Kamran Student, Tayyebeh , Ajami Ali , Taghiyar Sana , Deemeh Mohammad Reza 
Published: March 2019

Background: The effect of antioxidant therapy on sperm DNA fragmentation index (DFI) and achieving natural pregnancy were under debate. Very few studies have showed the rate of pregnancy rate after the antioxidant therapy due to ethical and technical limitations.
Objective: The aim of this cohort study was to determine the improvement rate of sperm DFI and natural pregnancy rate after the antioxidant therapy in infertile men.
Materials and Methods: 1645 infertile men were subjected for this study from May 2015 to December 2017. The Spermogram and sperm DFI were assessed using World Health Organization (WHO) 2010-based protocols and sperm chromatin structure assay (SCSA), respectively, in sperm samples before and after antioxidant therapy.
Results: The total sperm DFI improvement rate was 38.9% in the total population. Sperm DFI improvement had close correlation with total motility (r= 0.731, p= 0.001) and progressive motility improvement (r= 0.885, p= 0.001); 16.8% of individuals who completed antioxidant therapy for nine months achieved natural pregnancy.
Conclusion: The results of the current study suggested that SCSA along with spermogram might be a suitable option for the evaluation of fertility potential. In addition, antioxidant therapy may be useful for men with high levels of sperm DFI. However, the rate of pregnancy was still low and other treatment protocols such as assisted reproductive technology may be necessary.
Key words: Antioxidant, Reactive oxygen species, DNA fragmentation, Male infertility.
Subchronic effects of different doses of Zinc oxide nanoparticle on reproductive organs of female rats: An experimental study
Authors: Hosseini Seyed Mohammad , Moshrefi Amir Hossein , Amani Reza , Razavimehr Seyed Vahid , Aghajanikhah2 Mohammad Hasan , Sokouti Zahra , Holari Behnam Babaei 
Published: March 2019

Background: Zinc performs many biochemical and physiological functions; however, toxicological studies demonstrate that Nano-zinc oxide has harmful effects on human health and environmental species in high concentrations.
Objective: The aim of this study was to investigate the toxicity of zinc oxide nanoparticles on reproductive tissues of female rat.
Materials and Methods: Eighty female Wistar adult rats weighing 180–200 gr, divided into eight groups (n= 10 in each group) including control, sham (treated with saline), and six groups injected with different doses of zinc oxide nanoparticle with 10–30 nanometer size (4, 8, 25, 50, 100, and 200 mg/kg) twice a week for four weeks. At the end of the study, the rats were bled and slaughtered; the Ovary and Uterus were taken for histopathology studies and blood samples were transferred to the laboratory for biochemical analysis.
Results: Microscopic diagnoses in ovary tissue were included; increase in the corpus luteum, follicular cysts, inflammatory cells infiltration and fibrosis. Histopathological changes in ovary in a dose-dependent manner. In uterus tissue the lesions consisted; epithelial destruction, hyperplasia of endometrial glands. The Estrogen and Progesterone level in the serum of rats increased in low doses and reduced in a dose-dependent manner at high doses.
Conclusion: The results of the current study proved the toxicity of zinc oxide nanoparticles on the ovary and uterus organs at high concentrations, so further investigation is needed to reduce these effects.
Key words: Zinc oxide, Nanoparticles, Ovary, Uterus, Toxicity.
The relationship of the psychological coping and adjustment strategies of infertile women with the success of assisted reproductive technology
Authors: Golmakani Nahid , Zagami Samira Ebrahimzadeh , Esmaily Habibollah , Vatanchi Atiyeh , Kabirian Maryam 
Published: March 2019

Background: The success of assisted reproductive techniques plays a very important role in the quality of life of infertile couples and decreases the negative behavior states of infertility.
Objective: This study aimed at determining the relationship between psychological coping and adjustment strategies with the success of assisted reproductive technology (ART).
Materials and Methods: This correlational study was conducted on 204 women visiting Milad Infertility Center in Mashhad during 2015-2016. The research instruments included Fertility Adjustment Scale and Infertility Coping Strategies Scale. The positive result of two pregnancy tests within 48 hours was considered as the success of ART.
Results: The mean and standard division score of adjustment in the group achieved treatment success (34.3±8.2) exceeded the group failed (33.6±8.8), the difference was not statistically significant (p= 0.381). Also, there was no significant difference between groups in the median and interquartile range of total coping strategies 81 (13) vs. 79.5 (12.25), (p= 0.369). Based on the logistic regression model for one increased transferred embryo, the chance of getting pregnant is 1.3 times, and for each unit increase in FSH level, the chance of ART success decreases 18%.
Conclusion: The results of this study showed that there is no relationship between psychological coping and adjustment strategies with ART success. However, the number of transferred fetus and tirthday FSH are introduced as factors that are related to the success of ART.
Key words: Infertility, Psychological adjustment, Coping skills, (ART).
Protective effect of vitamin E on oxidative stress and sperm apoptosis in diabetic Mice
Authors: Khorramabadi Khadijeh Mirzaei , Talebi Ali Reza , Sarcheshmeh Abolghasem Abbasi , Mirjalili Aghdas 
Published: March 2019

Background: Generation of free radicals and oxidative stress are a major contributor to diabetes. These factors lead to the development of diabetic testicles disorders.
Objective: In this study, the protective effect of vitamin E on functional disorders associated with diabetes induced oxidative stress in male reproductive systems has been investigated.
Materials and Methods: Thirty-three adult male Mice were divided into control, diabetic, and untreated diabetic groups. Streptozotocin was used to induce diabetes. In the treated group, vitamin E was given to the Mice intraperitoneally for 30 days. Then, animals were anesthetized and sacrificed. Animal testicles were isolated and homogenized in phosphate buffer and used for measuring sperm count, motility and survival of sperm, MDA concentration and antioxidant capacity (TAC). Apoptosis was also performed with the TUNEL test.
Results: The results of reduction (12.03±98.11) TAC, MDA concentration (–28.5±2.58), sperm motility (unstable sperma= 86.4±7.48), sperm count (171.51), Sperm morphology (natural morphology= 49.69±31.93) and abnormal morphology (9.77±49.7) with increased oxidative damage. These changes were statistically significant in comparison with the control group for all variables other than MDA (p= 0.05). Treatment of vitamin E diabetic Mice improved the ability of antioxidants to prevent oxidative damage in the testicles, restore the sperm movement, and increase the number of normal sperm as well as TAC. The level of apoptosis in the treated group has decreased compared to the untreated group.
Conclusion: Vitamin E protects the reproductive system against diabetes mellitus. Therefore, it was concluded that vitamin E may be a suitable agent for protecting the sperm and testicular parameters against undesirable effects of diabetes.
Key words: Case-control study, Vitamin E, Diabetes treatment, Diabetic Syrian mice, Male reproductive dysfunction.
Performance Analysis of the Differences Restricted Access Window (RAW) on IEEE 802.11ah Standard with Enhanced Distributed Channel Access (EDCA)
Authors: Perbawa Mulki Nurullah , Perdana Doan , Bisono Yoseph Gustommy 
Published: March 2019

IEEE 802.11 standard is a WLAN (Wireless LAN) standard that has been used in all over the world. IEEE 802.11ah is the newer technology that designed to supports Internet of Things (IoT) and Machine-to-machine Communication (M2M). IEEE 802.11ah has a feature called Restricted Access Window (RAW) that capable to reduce power usage and have satisfying Quality of Service (QoS). In this research, Enhanced Distributed Channel Access (EDCA) is also applied. Same as RAW, EDCA also be able to affect QoS by modified the MAC Layer in 802.11 standard. This research used 3 different scenarios for RAW parameters: Modifying the number of RAW Group, Modifying the number of RAW Slot, and Comparing 2 Datamode. The EDCA Parameters that used in this research were: Contention Window and Arbitrary inter-frame Spacing Number. The values that expected to be the output in this research are: Delay, Throughput, Packet Delivery Ratio, Availability, and Reliability. After the research has been simulated, the results are: First, the lowest of average delay was Ngroup = 1, the highest of PDR was Ngroup = Nsta/2, and the highest of Throughput was Ngroup = Nsta/2. Second, the lowest of average delay was RAW Slot = 6, the highest of PDR were RAW Slot = 3 and 4, and the highest of Throughput was RAW Slot = 4. Third, the lowest of average delay was Datamode 3,9 Mbps BW 2 MHz, the highest of PDR was Dat mode 3,9 Mbps BW 2 MHz, and the highest of Throughput was Datamode 3,9 Mbps BW 2 MHz. Reliability, Availability, and Energy Consumption also can be affected by modifying RAW parameters, in 802.11ah Energy Consumption can be reduced by increasing the number of RAW Stations and RAW Groups.
Determination of sources of sulfur dioxide in confectionery
Authors: Kondratyev N. B. , Kazantsev E. V. , Osipov M. V. , Rudenko O. S. , Krylova E. N. 
Published: March 2019

Sulfur dioxide is used to ensure the safety of fruit raw materials and semi-finished products used for the production of confectionery. This preservative has allergenic properties. In accordance with the Technical Regulations of the Customs Union TR CU 022/2011 "Food products in terms of their labeling" the content of sulfur dioxide must be specified when labeling confectionery products, if its content exceeds 10 mg per kg. The definition of this preservative in raw materials, semi-finished products and confectionery in accordance with the current GOST 26811 “Confectionery. The iodometric method for determining the mass fraction of total sulfuric acid is often difficult because of the complexity of confectionery products, the formation of intensive staining of the solutions under study and the ability of sulfur dioxide to react with other components of the objects under study. Therefore, the task of developing methods with the optimization of the sample preparation stage of the samples under study, which allow one to fully determine sulfur dioxide in confectionery products, semi-finished products and raw materials, is relevant. The purpose of this work was to develop methods for determining sulfur dioxide in raw materials, semi-finished products and various names of confectionery products, as well as assessing the impact of these types of raw materials and semi-finished products on the content of sulfur dioxide in confectionery products. The results showed that sulfur dioxide is present in many raw materials. In mg per 1 kg of sugar in white sand from 1 to 9, molasses from 25 to 52, wheat flour from 9 to 15, starch from 3 to 17. In fruit raw materials from 5 to 545 mg per kg. In confectionery: marshmallow and marshmallow from 8 to 29, gingerbread from 6 to 25, biscuits from 0 to 16, chocolate from 8 to 13. Based on the data obtained, it can be concluded that the content of sulfur dioxide in raw materials and semi-finished confectionery products is a very wide range, which makes it necessary to control the quality of all raw materials entering the production. Sulfur dioxide, used as a preservative for fruit raw materials, can significantly increase the content of this allergen in confectionery made using such raw materials. Ensuring the level of sulfur dioxide less than 10 mg / kg is achieved using raw materials with low content of sulfur dioxide and technological methods.
Patterns of inhibition of the culture of Salmonella
Authors: Ilyuhina N. V. , Kolokolova A. Yu. 
Published: March 2019

Studies on the inhibition effectiveness of the initial degree of inoculation of the Salmonella microorganism were carried out. In this paper, the irradiation efficiency of model systems with electron beams with beam energy of 6.5 and 10 MeV was studied. The investigations were carried out at UELV-10-10-C-70 accelerator at Frumkin Institute of Physical Chemistry and Electrochemistry of Russian Academy of Sciences (IPCE RAS) with an average beam power of 6.5 MeV and at radiation-technological center with an electron accelerator UELR-10-10-40 at A.I. Burnazyan Federal Medical Biophysical Centre of Federal Medical Biological Agency with an average electron energy of 10 MeV. The conducted researches on studying and revealing the dependence of inhibition of pathogenic microflora, irradiation with different intensity, on the structure (density) of the studied samples, which model liquid and solid nutrient media. The study used a strain Salmonella enterica subsp. Enterica serovar Typhimurium. The results of the effective inhibition of the initial degree of contamination for the two plants were obtained. Studies have shown that the effectiveness of inhibition of Salmonella culture can vary depending on the characteristics of electron beams. For example, when samples were irradiated with electrons with energy of 6.5 and 10 MeV at doses from 3 to 7 kGy, various results of the inhibition effectiveness of Salmonella culture on media with different work were obtained. When processing samples with studied strains of cultures in the dose range from 4 to 5 kGy, there is an increase in the growth of microorganisms for all processing conditions. In the remaining studied ranges their inhibition are observed. It is important to take into account not only the effectiveness of the oppression of microflora on specific products, but also the efficiency of the installation for a specific sample.
Efficiency of synthesis of extracellular polysaccharides strains of Lipomyces yeast
Authors: Khusainov I. A. , Yakubov E. R. , Kanarskaya Z. A. , Kanarskiy A. V. , Maximova I. A. , Kachalkin A. V. 
Published: March 2019

The formation of extracellular polysaccharides is a fairly well-studied property of bacteria that is used for the industrial production of such extracellular bacterial as xanthan, dextran, gellan, hyaluronan, etc.. Polysaccharides synthesized by fungi are also widely used, such as schizophillan and scleroglucan. However, polysaccharides synthesized by yeast and yeast-like fungi have not yet found wide industrial application, with the exception of pullulan produced by Aureobasidium pullulans yeast, although there are a number of promising developments in the use of yeast polysaccharides in medicine. Yeast synthesizes polymers that contain mannans, glucans, phosphomannans, galactomannans, and glucuronoxylmannans. Polysaccharides produced by different species, and sometimes even by different strains of the same species, may differ in chemical composition and structure. Such a variety of composition and properties opens up great prospects for their use in various fields: medicine, chemical, food and cosmetic industries, as well as feed additives. In this regard, the search for new producers of polysaccharides is very relevant. Yeast of the genus Lipomyces is found in the soils of the southern and northern hemispheres of the Earth, except in the high-mountainous regions and tundra soils, where soil formation processes are in early stages of development, but the soils are rich in steppe and forest zones. As a result of the research, it turns out that from the point of view of biomass growth on the presented nutrient medium at the temperatures studied, the strains of the Lipomyces lipofer yeast КБП Y-6267 and КБП Y-6265 attract the most attention, especially at low temperatures. With an increase in temperature, the increase in biomass in these yeasts decreases markedly. As producers of extracellular polysaccharide, it is worth noting the КБП Y-6267 and КБП Y-6264 strains at 20 °C and the КБП Y-6268 strains and the КБП Y-6234 at 30 °C, which indicates the possibility of using for these purposes different species of the genus Lipomyces. At 30 °C, Lipomyces lipofer strains of the КБП Y-6268 and Lipomyces kononenkoae КБП Y-6234 had the highest enzyme activities, however, there was no relationship between enzyme activities, biomass gains and polysaccharide yields at low temperatures.