bluuebunny/crossref_metadata_2025_split huggingface dataset:
Values of welfare technologies: a qualitative study of how employees in Swedish care for older adults understand and justify the use of new technology
Authors: Lydahl Doris , Davidsson Anna 
Published: December 2024

Abstract
Background
In Swedish care for older adults, the use of welfare technology − for example, medicine-dispensing robots and GPS alarms − stimulates ongoing evaluations and negotiations. National policy documents, as well as previous research, indicate that these technologies are expected to improve the quality and efficiency of care but also involve potential or experienced challenges in providing high-quality care and the conditions for care workers − often portrayed as a conflict between political and caring organizational values. More research is thus needed on how the use of welfare technology is justified at central and municipal government levels as well as within care-providing organizations. This article aims to identify how the introduction and use of welfare technology is justified by employees and in policy documents pertaining to welfare technology in Swedish care for older people.

Methods
Qualitative interview data involving 37 individuals were collected in municipalities in southern Sweden. The interviewees were managers, administrative staff, and licensed practical nurses working in home care services or special housing for older adults, offering care 24/7. Policy documents and agreements of significance for Swedish welfare technology policy were analysed. The analysis focused on values promoted as significant for the provision of care and related to the use of welfare technology. Justifications and values were analysed as belonging to different ‘worlds of worth’, as either shared or conflicting, and contextually situated.

Results
The documents reflect values regarding, for instance, efficiency, independence, good working conditions, optimised use of human resources, and time to tend to relationships. The employees justified the use of welfare technologies with reference to optimisation and efficiency, activation and participation, dignity and freedom, social relationships, and good working conditions. These values represent three different worlds of worth − the industrial world, the civic world, and the domestic world − and are situated in employees’ everyday work practices.

Conclusions
Values presented by employees and in policy documents are shared rather than conflicting. However, employees emphasise values associated with the domestic world, downplaying industrial values. We argue that politicians and civil servants should consider the situated judgements made by employees and develop more bottom-up strategies. This requires an acknowledgement of the existing values hierarchy, where values of optimisation and efficiency are held in high esteem but to accommodate higher values rather than as end goals.
Supplementation of laying hens’ feed with Schizochytrium powder and its effect on physical and chemical properties of eggs
Authors: kiran Ifra , Umbreen Huma , Nisa Mahr un , Al-Asmari Fahad , Zongo Eliasse 
Published: December 2024

AbstractBiofortification enhances dietary quality and nutritional value using traditional marine microalga species, particularly docosahexaenoic acid (DHA), which is an essential n-3 fatty acid for human health. Eggs are natural fortified candidates. This study aimed to investigate the effect of dietary Schizochytrium powder on egg productivity, egg nutritional content, and fatty acid levels in laying hens. Hy-Line Brown laying hens (n = 150) were uniformly assigned to five groups for 52 days. The control group received no supplements, whereas the other four experimental groups were provided with varied amounts of schizochytrium powder. The experimental feed contained isonitrogenous (16.60%) and isocaloric metabolizable energy, 36.69 MJ/kg components. No significant differences were observed (p > 0.05) on different parameters such as average daily feed intake, feed conversion ratio, egg weight, and shell thickness. Enriching eggs significantly increased α-linolenic acid (ALA), eicosapentaenoic acid (EPA), and DHA levels (p < 0.001), while decreasing the n-6/-3 ratio in a dose-dependent manner, ensuring high quality and biological efficacy. A plateau point was maintained throughout the study period. In the first two weeks, increased DHA levels were observed in egg yolks when microalgae concentrations were elevated by 1%. The study found that powdered Schizochytrium limacinum served as a substitute for DHA in enhancing eggs with n-3 fatty acids.
Siwu decoction mitigates radiation-induced immune senescence by attenuating hematopoietic damage
Authors: Huang Mingyue , Ye Anping , Zhang Haoyu , Ru Yi , Bai Zhijie , Zhang Yanyan , Gao Yue , Ma Zengchun 
Published: December 2024

Abstract
Background
To investigate the long term effects of ionizing radiation (IR) on hematopoietic stem/progenitor cells (HSPCs), immune tissues and cells, and the effects of Siwu decoction (SWD) on immune senescence mice.

Methods
C57BL/6 J mice were exposed to 6.0 Gy 60Co γ irradiation. After 8-weeks of IR, SWD (5, 10, 20 g/kg/d) was administered for 30 days. The changes of HSPCs in bone marrow (BM) and T, B type lymphocyte and natural killer (NK) cells in spleen were detected by flow cytometry. The changes of peripheral blood cells were also examined. Hematoxylin–eosin staining were used to detect the pathological lesions of hippocampus, spleen and thymus tissues. Absolute mouse telomere length quantification qPCR assay kit was used to measure the telomere length of BM cells. The expression of factors associated with inflammation and aging such as p16, β-galactosidase in spleen, thymus and BM was determined.

Results
Administration of SWD could increase the proportion of LSK (Lin−, Sca-1 + , c-Kit−), multipotent progenitor cells and multipotent progenitor cells and decrease the proportion of common myeloid progenitors and granulocyte–macrophage progenitors in BM. The proportion of B cells and NK cells in spleen and the content of white blood cells, red blood cells, hemoglobin, lymphocytes and eosinophils in peripheral blood were increased, at the same time, the proportion of neutrophils and monocytes was reduced by SWD. The pathological lesions of hippocampus, spleen and thymus were improved. The expression of p16 and β-galactosidase in spleen, thymus and BM was reduced and shortening of the telomere of BM cells was inhibited after administration. In addition, SWD could reduce the content of Janus activated kinase (JAK) 1, JAK2 and signal transducer and activator of transcription 3 (STAT3) in BM and spleen.

Conclusions
SWD could slow down IR-induced immune senescence by improving hematopoietic and immunologic injury. SWD might reduce the inflammation level of BM hematopoietic microenvironment by acting on JAK/STAT signaling pathway, while the immune damage of mice was improved by affecting the differentiation of HSPCs. The remission of hematopoietic and immunologic senescence was further demonstrated at the overall level.

Graphical Abstract
Characterization of tumor-infiltrating lymphocytes and their spatial distribution in triple-negative breast cancer
Authors: Han Eunkyung , Choi Hye Yeon , Kwon Hyun Jung , Chung Yul Ri , Shin Hee-Chul , Kim Eun-Kyu , Suh Koung Jin , Kim Se Hyun , Kim Jee Hyun , Park So Yeon 
Published: December 2024

Abstract
Background
The tumor immune microenvironment, particularly tumor-infiltrating lymphocytes (TILs), plays a critical role in disease progression and treatment response in triple-negative breast cancers (TNBCs). This study was aimed to characterize the composition of TILs and investigate their clinicopathological and prognostic significance with a special focus on the spatial distribution of TILs in TNBCs.

Methods
We analyzed TNBC samples through PanCancer Immune Profiling using NanoString nCounter assays to identify immune-related genes that are expressed differentially in relation to TIL levels and evaluated protein expression of selected markers through immunohistochemical staining on tissue microarrays. For a comprehensive assessment of the expression of cytotoxic T lymphocyte (CTL) and natural killer (NK) cell markers, a CTL-NK score was devised based on CD8+, CD56+, CD57+, GNLY+, and GZMB+ TIL levels.

Results
Gene expression analysis revealed significant upregulation of CTL and NK cell-associated genes including GNLY, KLRC2, and GZMB in TIL-high TNBCs. Immunohistochemical validation confirmed that TNBCs with higher TILs had a greater amount of CD56+, CD57+, GNLY+, and GZMB+ TILs not only in absolute number but also in proportion relative to CD4+ or CD8+ TILs. High TIL and its subset (CD4+, CD8+, CD56+, CD57+, GNLY+, and GZMB+ TIL) infiltration correlated with favorable clinicopathological features of tumor. In survival analysis, high CTL-NK score was found to be an independent prognostic factor for better disease-free survival (DFS) of the patients. Furthermore, uniformly high TIL infiltration was linked to better DFS, whereas cases with heterogeneous TIL infiltration showed no difference in survival compared to those with uniformly low TIL infiltration.

Conclusion
Our study showed that CTL and NK cell-associated gene expression and protein levels differ significantly according to TIL levels and that CTL-NK score and distribution of TILs within tumors have a prognostic value. These findings emphasize the importance of CTLs and NK cells as well as the spatial uniformity of TIL infiltration in clinical outcome of TNBC patients, providing valuable insights for refining prognostic assessments and guiding immunotherapeutic strategies.
Whole-genome resequencing to investigate the genetic diversity and mechanisms of plateau adaptation in Tibetan sheep
Authors: Li Xue , Han Buying , Liu Dehui , Wang Song , Wang Lei , Pei Quanbang , Zhang Zian , Zhao Jincai , Huang Bin , Zhang Fuqiang , Zhao Kai , Tian Dehong 
Published: December 2024

Abstract
Introduction
Tibetan sheep, economically important animals on the Qinghai–Tibet Plateau, have diversified into numerous local breeds with unique characteristics through prolonged environmental adaptation and selective breeding. However, most current research focuses on one or two breeds, and lacks a comprehensive representation of the genetic diversity across multiple Tibetan sheep breeds. This study aims to fill this gap by investigating the genetic structure, diversity and high-altitude adaptation of 6 Tibetan sheep breeds using whole-genome resequencing data.

Results
Six Tibetan sheep breeds were investigated in this study, and whole-genome resequencing data were used to investigate their genetic structure and population diversity. The results showed that the 6 Tibetan sheep breeds exhibited distinct separation in the phylogenetic tree; however, the levels of differentiation among the breeds were minimal, with extensive gene flow observed. Population structure analysis broadly categorized the 6 breeds into 3 distinct ecological types: plateau-type, valley-type and Euler-type. Analysis of unique single-nucleotide polymorphisms (SNPs) and selective sweeps between Argali and Tibetan sheep revealed that Tibetan sheep domestication was associated primarily with sensory and signal transduction, nutrient absorption and metabolism, and growth and reproductive characteristics. Finally, comprehensive analysis of selective sweep and transcriptome data suggested that Tibetan sheep breeds inhabiting different altitudes on the Qinghai–Tibet Plateau adapt by enhancing cardiopulmonary function, regulating body fluid balance through renal reabsorption, and modifying nutrient digestion and absorption pathways.

Conclusion
In this study, we investigated the genetic diversity and population structure of 6 Tibetan sheep breeds in Qinghai Province, China. Additionally, we analyzed the domestication traits and investigated the unique adaptation mechanisms residing varying altitudes in the plateau region of Tibetan sheep. This study provides valuable insights into the evolutionary processes of Tibetan sheep in extreme environments. These findings will also contribute to the preservation of genetic diversity and offer a foundation for Tibetan sheep diversity preservation and plateau animal environmental adaptation mechanisms.
A systematic review and meta-analysis of the prevalence and risk factors of type 2 diabetes mellitus in Nigeria
Authors: Olamoyegun Michael Adeyemi , Alare Kehinde , Afolabi Samson Adedeji , Aderinto Nicholas , Adeyemi Taiwo 
Published: December 2024

Abstract
Background
Type 2 diabetes mellitus (T2DM) is a major global non-communicable disease, leading to increased morbidity and mortality. Its prevalence in Nigeria is driven by various risk factors. This review assesses the national and regional prevalence and risk factors of T2DM in Nigeria.

Methods
Following PRISMA guidelines, electronic databases (PubMed, Scopus, Google Scholar, African Journals Online) and gray literature were searched for English-language studies. The quality of the included studies was assessed using the Newcastle–Ottawa Scale. Data were extracted with Microsoft Excel and analyzed using Stata version 16 software. Random effect meta-regression analysis at 95% CI was used to assess pooled prevalence and risk factors. Heterogeneity was determined using the I2 statistic, and publication bias was evaluated with a funnel plot.

Results
Sixty studies from different Nigerian geopolitical zones met eligibility criteria, with a total sample size of 124,876 participants and a mean age of 48 ± 9.8 years. The pooled prevalence of T2DM in Nigeria was 7.0% (95% CI: 5.0-9.0%). Moderate publication bias was observed. The South-south zone had the highest prevalence at 11.35% (95% CI: 4.52-20.72%), while the North-central zone had the lowest at 2.03% (95% CI: 1.09-3.40%). Significant risk factors included family history (9.73), high socioeconomic status (6.72), physical inactivity (5.92), urban living (4.79), BMI > 25/m2 (3.07), infrequent vegetable consumption (2.68), and abdominal obesity (1.81).

Conclusion
The prevalence of T2DM in Nigeria (7.0%) nearly doubled the 2019 International Diabetes Federation estimate (3.7%) and shows a 21.3% increase from the 2019 review. Efforts should focus on modifying identified risk factors to reduce prevalence and prevent complications.
Chemical profiling and clustering of various dried cannabis flowers revealed by volatilomics and chemometric processing
Authors: Janta Pannipa , Vimolmangkang Sornkanok 
Published: December 2024

AbstractCannabis flower scent is one of the key characteristics of the cannabis plant. The diverse scents impact user experiences and offer medicinal benefits. These scents originate from volatile compounds, particularly terpenes and terpenoids. This study characterized the volatile profile of 19 different dried cannabis flowers using gas chromatography-mass spectrometry coupled with headspace-solid phase microextraction (HS-SPME-GC-MS). A total of 75 compounds were identified, including alcohols, aldehydes, benzenes, esters, ketone, monoterpenes, monoterpenoids, sesquiterpenes, and sesquiterpenoids. Cluster analysis was able to group the 19 cannabis cultivars into five clusters based on volatile chemotypes using chemometric techniques of hierarchical cluster analysis (HCA) and principal component analysis (PCA). Potential discriminant markers of each cultivar were then analyzed using a supervised partial least squares discriminant analysis (PLS-DA) verified through Variable Importance in Projection values (VIP), identifying twenty discriminant markers. In addition, the correlations among 75 volatile compounds were also obtained. The findings of this study provide a valuable database of single cannabis cultivars, useful for identifying individual strains and verifying their quality. Clustering the cultivars by volatile chemotype can be used for the classification of cannabis in the market. The results of this study are expected to be a starting point for further cannabis breeding programs to expand knowledge of this plant. Furthermore, the proposed method is applicable to other aroma plants in the future.
Decolorizing of seaweed extract by electrocoagulation
Authors: Akoetey Winifred , Caton Simon , Lai Edward , Hosseinian Farah 
Published: December 2024

AbstractElectrocoagulation (EC) is a technique commonly used in wastewater treatment to remove biological and chemical contaminants, but the process has the potential to be used in clarifying plant extracts for the isolation and identification of secondary metabolites. Seaweed extracts contain copious amounts of chlorophyll and other pigments that obscure the characterization of secondary metabolites such as phenolic acids and flavonoids. In place of conventional methods that utilize solvents, EC can potentially be applied to clarify and fractionate extracts. In this research, an EC duration of 30 min (22 V, 0.3–0.5A) with aluminum electrodes resulted in a significant decrease, about 76%, of chlorophyll and 70% of carotenoids from seaweed extract measured at 666 nm and 410 nm. The decrease in extract green and yellow color intensity also mirrored a decrease in total phenolic content (TPC) of the extract from 54 ± 1.55 mg GAE/g DW to 3.2 ± 0.01 mg GAE/g DW after 30 min of EC. However, the phenolic acid profile of the extract after electrocoagulation via HPLC-RP indicated the removal of an interference probably caused by polymeric compounds from the extract, thus leaving the simple phenolic acids in solution for detection. The major phenolic acids detected in seaweed crude extract were p-coumaric, o-coumaric, ferulic and syringic acid. Flavonoids detected included catechin, epicatechin, quercetin-3-glucoside and rutin. The results of this study show the potential of replacing conventional plant extract purification methods with a green method that requires no additional solvent.
Graphical Abstract
Oncology and Primary Care Involvement in Breast Cancer Survivorship Care More Than 5 Years After Initial Treatment
Authors: Radhakrishnan Archana , Furgal Allison K.C. , Hamilton Ann S. , Ward Kevin C. , Veenstra Christine , Hawley Sarah T. , Katz Steven J. , Lee Joy L. , Wallner Lauren P. 
Published: December 2024

PURPOSE
Very little is known about primary care involvement in the care of cancer survivors beyond the initial 5 years post-treatment when transitioning to primary care is guideline-recommended for many survivors.

METHODS
The ICanCare study is a longitudinal survey of women diagnosed with breast cancer in 2014-2015 identified in the Georgia and Los Angeles SEER registries. Women were surveyed during initial treatment and again approximately 6 years later in survivorship (2021-2022; n = 1,412, 60% response rate). Respondents were asked which provider led their survivorship care (oncology, shared care, primary care provider [PCP]) and PCP management of eight common survivorship issues (range, 0-32). Multivariable-adjusted associations of participant characteristics with survivorship care delivery and PCP management of survivorship issues were evaluated using logistic and linear regression models, respectively.

RESULTS

Over half of the women (57.2%) reported oncologist-led survivorship care delivery, 20.5% shared care, and 22.4% PCP-led. PCP management of survivorship issues was moderate (mean, 11.4, 95% CI, 11.0 to 11.8) and was highest (often/always discussed) for medication management (50.1%), improving physical activity (49.1%), and chronic disease management (43.6%). Greater confidence in PCP's ability to manage survivorship care (
v
no or little confidence) was associated with PCP-led delivery (
P
= .01) and more PCP management of survivorship issues (
P
< .0001).

CONCLUSION
In this diverse cohort of breast cancer survivors, primary care involvement in survivorship care delivery and management of common survivorship issues remains low even more than 5 years after completing treatment and little variation was seen across survivor sociodemographic or clinical characteristics. Confidence in PCP ability to manage survivorship care was found to be important and may represent an opportunity to foster transition to primary care-led survivorship care delivery.
KRAS
Variants Are Associated With Survival Outcomes and Genomic Alterations in Biliary Tract Cancers
Authors: Gelfer Rebecca , Gulla Aiste , Kalvin Hannah L. , Song Yi , Harding James , Abou-Alfa Ghassan K. , O'Reilly Eileen M. , Park Wungki , Chandwani Rohit , Wei Alice , Kingham Peter , Drebin Jeffrey , Balachandran Vinod , D'Angelica Michael , Soares Kevin , Gonen Mithat , Jarnagin William R. 
Published: December 2024

PURPOSE

KRAS
variants are associated with poor outcomes in biliary tract cancers (BTCs). This study assesses the prevalence of
KRAS
variants and their association with survival and recurrence in patients with intrahepatic cholangiocarcinoma (IHC), extrahepatic cholangiocarcinoma (EHC), and gallbladder adenocarcinoma (GB).

METHODS
In this cross-sectional, single-institution study at Memorial Sloan Kettering, tumors from 985 patients treated between 2004 and 2022 with IHC, EHC, and GB who underwent either curative-intent resection or were treated with chemotherapy for unresectable disease were used for targeted sequencing.

RESULTS

Of the 985 patients sequenced, 15% had a
KRAS
mutation. Five hundred and seventy-two had unresectable disease (n = 395 IHC, n = 71 EHC, n = 106 GB) and 413 were treated with curative-intent resection (n = 175 IHC, n = 119 EHC, and n = 119 GB). Median follow-up time was 18 months (IQR, 11-31).
KRAS G12D
mutations were most common in IHC (38%) and EHC (37%) tumors. Mutations in
SF3B1
co-occurred with mutant
KRAS
in IHC and EHC, with comutant resectable patients having worse survival after adjusting for tumor type (hazard ratio [HR], 4.04 [95% CI, 1.45 to 11.2];
P
= .007).
KRAS G12
mutations were associated with worse survival in patients with IHC compared with wild-type (WT) or other
KRAS
mutations, regardless of resection status (unresectable
P
< .001, resectable
P
= .011). After adjusting for clinical covariates,
KRAS
G12 mutations remained a prognostic indicator for patients with IHC compared with WT (HR, 1.99 [95% CI, 1.41 to 2.80];
P
< .001).

CONCLUSION

The adverse impact of
KRAS
mutations in BTC is driven by G12 alterations in patients with IHC regardless of resection status, which was not observed in GB or EHC. There are unique comutational partners in distinct BTC subsets. These differences have important clinical implications in the era of KRAS-targeted therapeutics.
Narratology Talks to the Talking Cure in Persuasion
Authors: Jones Wendy 
Published: December 2024

Abstract
This essay puts Persuasion in conversation with psychotherapeutic approaches to Austen and cognitive literary studies (more broadly, approaches that draw on the sciences of mind). Narrative therapy provides a heuristic for Persuasion, accounting for Anne Elliot's recovery from melancholy to achieve a sense of well-being. In line with the goals of narrative therapy, she learns to tell a different story about herself than the one that has oppressed her since her broken engagement. The novel represents the therapeutic elements involved in such reenvisioning, including validation; holding; the availability of alternative, better narratives as models; and awareness of the cultural factors that have contributed to her state of mind. Conversely, narratology accounts for the representation of Anne's experience, showing how literary devices, including embedded narrative, focalization, and voice, convey Anne's transformation from melancholy and passive spinster to active and vital woman who defends her actions and renews her engagement on better terms. This transformation depends not only on interpersonal exchanges but also on her realization that cultural “master narratives” infiltrate our most intimate stories.