bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prediction of Lymph Node Metastasis in Breast Cancer by Gene Expression and Clinicopathological Models: Development and Validation within a Population-Based Cohort
Authors: Dihge Looket , Vallon-Christersson Johan , Hegardt Cecilia , Saal Lao H. , Häkkinen Jari , Larsson Christer , Ehinger Anna , Loman Niklas , Malmberg Martin , Bendahl Pär-Ola , Borg Åke , Staaf Johan , Rydén Lisa 
Published: July 2019

Abstract

Purpose:
More than 70% of patients with breast cancer present with node-negative disease, yet all undergo surgical axillary staging. We aimed to define predictors of nodal metastasis using clinicopathological characteristics (CLINICAL), gene expression data (GEX), and mixed features (MIXED) and to identify patients at low risk of metastasis who might be spared sentinel lymph node biopsy (SLNB).
Experimental Design: Breast tumors (n = 3,023) from the population-based Sweden Cancerome Analysis Network–Breast initiative were profiled by RNA sequencing and linked to clinicopathologic characteristics. Seven machine-learning models present the discriminative ability of N0/N+ in development (n = 2,278) and independent validation cohorts (n = 745) stratified as ER+HER2−, HER2+, and TNBC. Possible SLNB reduction rates are proposed by applying CLINICAL and MIXED predictors.

Results:
In the validation cohort, the MIXED predictor showed the highest area under ROC curves to assess nodal metastasis; AUC = 0.72. For the subgroups, the AUCs for MIXED, CLINICAL, and GEX predictors ranged from 0.66 to 0.72, 0.65 to 0.73, and 0.58 to 0.67, respectively. Enriched proliferation metagene and luminal B features were noticed in node-positive ER+HER2− and HER2+ tumors, while upregulated basal-like features were observed in node-negative TNBC tumors. The SLNB reduction rates in patients with ER+HER2− tumors were 6% to 7% higher for the MIXED predictor compared with the CLINICAL predictor accepting false negative rates of 5% to 10%.

Conclusions:
Although CLINICAL and MIXED predictors of nodal metastasis had comparable accuracy, the MIXED predictor identified more node-negative patients. This translational approach holds promise for development of classifiers to reduce the rates of SLNB for patients at low risk of nodal involvement.
Glibenclamide Targets Sulfonylurea Receptor 1 to Inhibit p70S6K Activity and Upregulate KLF4 Expression to Suppress Non-Small Cell Lung Carcinoma
Authors: Xu Kexin , Sun Geng , Li Min , Chen Hongling , Zhang Zuhao , Qian Xixi , Li Ping , Xu Lin , Huang Wenbin , Wang Xuerong 
Published: July 2019

Abstract
Sulfonylurea receptor 1 (SUR1) is the regulatory subunit of ATP-sensitive potassium channels (KATP channels) and the receptor of antidiabetic drugs, such as glibenclamide, which induce insulin secretion in pancreatic β cells. However, the expression and role of SUR1 in cancer are unknown. In this study, we found that SUR1 expression was elevated in human non–small cell lung carcinoma (NSCLC) tissues and cell lines. SUR1 silencing suppressed the growth of NSCLC cells, while SUR1 overexpression promoted cell growth. Targeting SUR1 with glibenclamide suppressed cell growth, cell-cycle progression, epithelial–mesenchymal transition (EMT), and cell migration. Moreover, SUR1 directly interacted with p70S6K and upregulated p70S6K phosphorylation and activity. In addition, glibenclamide inhibited p70S6K, and overexpression of p70S6K partially reversed the growth-inhibiting effect of glibenclamide. Furthermore, glibenclamide upregulated the expression of the tumor suppressor Krüppel-like factor 4 (KLF4), and silencing KLF4 partially reversed the inhibitory effect of glibenclamide on cell growth, EMT, and migration. We found that SUR1 targeted p70S6K to downregulate KLF4 expression by enhancing DNA-methyltransferase 1–mediated methylation of the KLF4 promoter. Finally, in xenograft mouse models, SUR1 expression silencing or glibenclamide treatment inhibited the growth of A549 tumors, downregulated p70S6K activity, and upregulated KLF4 expression. These findings suggested that SUR1 expression was elevated in some NSCLC tissues and functioned as a tumor enhancer. Targeting SUR1 with glibenclamide inhibited NSCLC through downregulation of p70S6K activity and subsequent upregulation of the expression of the tumor suppressor gene KLF4. SUR1 can be developed as a new target for cancer therapy and glibenclamide has potential anticancer effects.
High-Resolution History: Downscaling China’s Climate from the 20CRv2c Reanalysis
Authors: Amato R. , Steptoe H. , Buonomo E. , Jones R. 
Published: July 2019

AbstractA Met Office Hadley Centre regional climate model, HadRM3P, is used to dynamically downscale the NOAA Twentieth Century Reanalysis, version 2c (20CRv2c), to generate a fine-resolution reconstruction of China’s climate from 1851 to 2010. The downscaled dataset has a small warm and seasonal wet bias (1.4°C; 0.9 mm day−1) relative to recent observations but otherwise represents spatial and temporal trends realistically. Analysis focused on temperature and precipitation shows that downscaling 20CRv2c is found to improve its representation of China’s climatological annual cycle, particularly over areas with sparse observational coverage such as the Tibetan Plateau. The downscaled dataset better represents the interannual variability and trends in observed temperature since 1901 and suggests that China has experienced a significant and sustained increase in temperature of 0.05°C (10 yr)−1 since the 1850s. Chinese precipitation trends have not changed significantly in the recent past or over the past 160 years. This analysis serves as an initial yet imperative step toward improving in-depth understanding of the characteristics and multidecadal drivers of high-impact events over China such as heat waves, droughts, and extreme precipitation.
Major Moisture Pathways and Their Importance to Rainy Season Precipitation over the Sanjiangyuan Region of the Tibetan Plateau
Authors: Zhang Yu , Huang Wenyu , Zhong Deyu 
Published: July 2019

Abstract
Knowledge of the quantitative importance of moisture transport pathways of the Sanjiangyuan region (known as the “water tower” of China) can provide insights into the regional atmospheric branch of the hydrological cycle over the Sanjiangyuan region. A combined method with a clustering algorithm [Hierarchical Density-Based Spatial Clustering of Applications with Noise (HDBSCAN)] and a Lagrangian moisture source diagnostic is developed to identify the major moisture transport pathways and quantify their importance to three types of consecutive precipitation events—extreme precipitation (EP) events, moderate precipitation (MP) events, and extreme aridity (EA) events—for the Sanjiangyuan region during the rainy season (June–September 1960–2017). The results indicate that moisture paths from the northwest covering northwest China and central Asia (the N.W. pathway) and moisture paths from southern and southeastern China (the S.S. pathway) are stable moisture transport pathways during EP and MP events [importance (precipitation contribution in percentage): N.W. pathway, 18.4% (EP), 32.2% (MP); S.S. pathway, 25.9% (EP), 28.5% (MP)]. Affected by the western edge of a significant anticyclone anomaly centered around 35°N, 115°E, the moisture paths via the Bay of Bengal (the B.B. pathway) can reach the target region and become a supplementary moisture contributor (14.9%) to EP events. Moisture paths via the Arabian Sea and Indian peninsula (the A.I. pathway) are also active but the contributions are limited [4.9% (EP) and 5.6% (MP)]. For EA events, the fast-moving trajectories from farther western Asia (the F.W. pathway) play a dominant role and all major moisture pathways (F.W., N.W., and S.S. pathways) carry limited moisture to the target region.
Estimates of Testing for Latent Tuberculosis Infection and Cost, United States, 2013
Authors: Marks Suzanne M. , Woodruff Rachel Yelk , Owusu-Edusei Kwame , Asay Garrett R. Beeler , Hill Andrew N. 
Published: July 2019

Objectives: Tracking trends in the testing of latent tuberculosis infection (LTBI) can help measure tuberculosis elimination efforts in the United States. The objectives of this study were to estimate (1) the annual number of persons tested for LTBI and the number of LTBI tests conducted, by type of test and by public, private, and military sectors, and (2) the cost of LTBI testing in the United States. Methods: We searched the biomedical literature for published data on private-sector and military LTBI testing in 2013, and we used back-calculation to estimate public-sector LTBI testing. To estimate costs, we applied Medicare-allowable reimbursements in 2013 by test type. Results: We estimated an average (low-high) 13.3 million (11.3-15.4 million) persons tested for LTBI and 15.3 million (12.9-17.7 million) LTBI tests, of which 13.2 million (11.1-15.3 million) were tuberculin skin tests and 2.1 million (1.8-2.4 million) were interferon-γ release assays (IGRAs). Eighty percent of persons tested were in the public sector, 18% were in the private sector, and 2% were in the military. Costs of LTBI tests and of chest radiography totaled $314 million (range, $256 million to $403 million). Conclusions: To achieve tuberculosis elimination, millions more persons will need to be tested in all sectors. By targeting testing to only those at high risk of tuberculosis and by using more specific IGRA tests, the incidence of tuberculosis in the United States can be reduced and resources can be more efficiently used.
Dimensional Analysis in Economics: A Study of the Neoclassical Economic Growth Model
Authors: Texocotitla Miguel Alvarez , Alvarez-Hernández M. David , Alvarez-Hernández Shaní Eneida 
Published: July 2019

The fundamental purpose of the present research article is to introduce the basic principles of dimensional analysis in the context of the neoclassical economic theory, in order to apply such principles to the fundamental relations that underlay most models of economic growth. In particular, basic instruments from dimensional analysis are used to evaluate the analytical consistency of the neoclassical economic growth model. The analysis shows that an adjustment to the model is required in such a way that the principle of dimensional homogeneity is satisfied. JEL: A12, C02, C65, O40
Target Capture/Next-Generation Sequencing for Nonsyndromic Cleft Lip and Palate in the Japanese Population
Authors: Shibano Masayasu , Watanabe Akira , Takano Nobuo , Mishima Hiroyuki , Kinoshita Akira , Yoshiura Koh-ichiro , Shibahara Takahiko 
Published: July 2019

Objective: The pathogenesis of nonsyndromic cleft lip with or without cleft palate (NSCL ± P) and nonsyndromic cleft palate only (NSCP) may be associated with genetic factors. Although some predisposing genes/loci have been reported, their attributable risk is too small to be clinically meaningful. To clarify the genetic causes and mechanisms of NSCL±P or NSCP, we conducted mutation analysis of target genes using a next-generation sequencing (NGS) approach. Methods: The target genes, IRF6, WNT5A, WNT9B, TP63, MSX1, TFAP2A, PAX9, DLX3, DLX4, and MN1, were selected based on previous reports of potential associations with the development of NSCL±P or NSCP from genome-wide association studies and candidate gene analyses. Mutation analysis was conducted using NGS on 74 Japanese trios (patient and parents) and 18 Japanese patients only families. Results: We detected single-nucleotide variants (SNVs) for 7 genes: IRF6, DLX4, WNT5A, TFAP2A, WNT9B, TP63, and PAX9. The SNVs found on IRF6 and DLX4 were missense mutations, whereas those identified on WNT5A, TFAP2A, WNT9B, TP63, and PAX9 were rare variants in the noncoding region; no de novo mutation was identified in the trio samples. The amino acid change on DLX4 was detected within the highly conserved homeodomain and was predicted to have a deleterious impact on the protein function by in silico analysis. Conclusions: The DLX4 missense mutation c.359C>T (Pro120Leu) was found in 1 Japanese patient with NSCL±P and was located in the homeodomain region. This mutation likely plays a role in the development of NSCL±P in the Japanese population.
A Rare Case of 7 Simultaneous Arterial Dissections and Review of The Literature
Authors: Amitai Komem Daphna , Sukenik Halevy Rivka , Griton Yigal , Shnaker Ali , Levy Yair , Weissmann Jonathan , Fajer Simone 
Published: July 2019

Objective: Spontaneous multiple artery dissection is a relatively rare phenomenon. Early clinical signs are often nonspecific, making it difficult to diagnose. Case Report: This is a case of a 51-year-old female who presented with spontaneous dissection of 4 visceral arteries, both iliac arteries, and of the right internal carotid artery. The patient underwent urgent successful endovascular repair. Later complications included acute respiratory distress syndrome and pneumonia after massive blood transfusion. She recovered gradually and was discharged after 21 days. Due to this rare presentation, genetic investigation was performed in search of a connective tissue disorder. Results revealed a new COL3A1 subtype mutation. The pathogenicity of this variant remains unclear. Conclusion: We recommend a high index of suspicion for visceral artery dissection in the differential diagnosis for abdominal pain with concurrent uncontrolled hypertension. Early diagnosis and intervention are crucial to reducing the mortality rate.
A Case of Exploding Markets: Latin American and South Asian Literary “Booms”
Authors: Kantor Roanne L. 
Published: July 2019

Abstract
This article seeks to explain the recent popularity of South Asian Anglophone literature (beginning in 1981 and peaking between 1998 and 2008) in light of the boom in Latin American literature of the 1960s. It argues that the phenomenon of regional literary “booms” shares features across both eras, and that a unified theory of booms is increasingly important to understanding the way contemporary literature circulates around the globe. Scholarship about both eras has tended to coalesce around three types of boom-driving agents: “creators,” “contexts,” and “curators.” Within that broader agreement, however, scholarship about the South Asian boom has tended to overemphasize the political symbolism of recent South Asian Anglophone literature and its global popularity, while under-emphasizing the political realities that create the conditions under which that literature became popular. This line of criticism has come at the expense of attention to literature’s other dimensions as a cultural object, as well as contextual explanations of popularity involving the role of governments, demographics, and market flows. The more diverse scholarship on the Latin American boom offers a corrective with insights for both the future of South Asian Anglophone literature and the field of World Literature.
Mutant City: On Partial Transformations in Three Johannesburg Narratives
Authors: Wright Timothy 
Published: July 2019

Abstract
Since the fall of the apartheid regime, critical discourse on and popular imaginations of South Africa have focused with renewed intensity on the city of Johannesburg: its schizophrenic social organization, its fragmented geography, its “citadelization,” its “architecture of fear,” and its development within networks of global capital, all indexes of the ultimate failure of the nation to move beyond its segregated past. In this essay, I will focus on representations of Johannesburg's mutancy, a concept that foregrounds its temporal movements rather than its spatial calcification. In particular, I examine the uses to which the tropes of mutation and the figure of the mutant are put in a number of recent Johannesburg narratives. Mutation here is a logic of discontinuous transformation, distinct from “hybridity,” concerned less with mimicry and in-betweenness than with emergent forms of life in spaces where ideological forces have ceded to material ones. The speculative mutations in these texts give body to various forms of emergent, unconceptualized, or fantastic subjectivities, homologous with but not reducible to the “real” mutations taking place in South African urban space. I am ultimately interested in how these subjectivities inform various imaginations of futurity—catastrophic, deconstructive, and regenerative—within a country in which, as Imraan Coovadia has written, “the conditions for transcending the present are hardly to be conceived” (51).
The Shield-Shaped Jade Pendant
Authors: Erickson Susan N. 
Published: July 2019

Abstract
Objects carved of jade often were placed in Han-dynasty burials of people of high rank. This article focuses on a small, shield-shaped (or “heart-shaped”) pendant frequently found near the deceased. The development of the type is examined through its appearance in tombs dating to the early Western Han through the end of the Eastern Han and extending into the immediate post-Han period. The typology of the pendant and its surface decoration are analyzed. This type of jade pendant resurfaced in the Song dynasty, but its most significant resurgence is during the Ming and early Qing dynasties, although by then its decorative features, as recorded in illustrations in books, appear to be more important than its use in burials. The article also explores the foundational years of collecting Chinese art in the West by individuals such as the sinologist Berthold Laufer, as well as other scholars of Chinese art, as they began to understand the shield-shaped pendant's origin as a Han-dynasty artifact and to explore its significance.
Beyond the Spectacular
Authors: Pereira Charmaine 
Published: July 2019

AbstractThe aim of this essay is to interrogate gender relations in the wake of the Boko Haram insurgency in a way that recognizes continuities as well as discontinuities across multiple dimensions of social relations. The essay begins by outlining the changing trajectory of the Boko Haram insurgency and scholarly efforts to understand it as a social phenomenon. The second section discusses how research and media recognition of Boko Haram’s violence in relation to women led to a focus on spectacular events, such as mass abductions and suicide bombings. It is critical to recognize the politics of visibility and nonvisibility regarding women in the gendered dynamics set in motion by Boko Haram’s spectacles of violence. Finally, the essay points to ways in which feminist analyses of conflict and militarism throw light on the more suppressed yet critical dimensions of gender relations that surface in the wake of the Boko Haram insurgency.
Training Load and Energy Expenditure during Military Basic Training Period
Authors: JURVELIN HEIDI , TANSKANEN-TERVO MINNA , KINNUNEN HANNU , SANTTILA MATTI , KYRÖLÄINEN HEIKKI 
Published: July 2019

ABSTRACT

Purpose
To compare training load and energy expenditure during an 8-wk military basic training (BT) period among individuals having different fitness level using objective measurements in an authentic environment.

Methods
Thirty-four voluntary male conscripts (age, 19.1 ± 0.3 yr) were divided into three training groups (inactive, moderate, active) by their reported physical activity (PA) level evaluated by the International Physical Activity Questionnaire (IPAQ) before military service. Maximal oxygen uptake (V˙O2max) and HR were determined by maximal treadmill test in the beginning and after 4 and 7 wk of BT. During BT, HR monitors and accelerometers were used to measure PA and energy expenditure. The HR data were used to calculate the training load (TRIMP, training impulse) for each day, week, and the whole BT period.

Results
Training load of BT was comparable to training of competitive athletes at the highest level. The training groups differed (P < 0.001–0.05) in terms of V˙O2max to each other (inactive, 36 ± 6; moderate, 42 ± 6; active, 48 ± 6 mL·kg−1·min−1). The conscripts in the inactive group were the most loaded during the study period (TRIMPinactive 12,393 ± 2989 vs TRIMPmoderate 10,252 ± 1337, P < 0.05 and TRIMPactive 8444 ± 2051, P < 0.01). The PA intensity of different military tasks during the BT period were low or moderate (<6 METs).

Conclusions
The remarkable training load during BT period is comparable to the training loads of professional athletes participating 3 wk of cycling competition. The training load in BT period was, however, primarily due to duration of low-intensity activities including only some high-intensity military activities. In the future, measuring the training load during the military service is recommended to customize the physical training for conscripts regarding his/her fitness level as much as possible.
Muscle Glycogen Content during Endurance Training under Low Energy Availability
Authors: KOJIMA CHIHIRO , ISHIBASHI AYA , TANABE YOKO , IWAYAMA KAITO , KAMEI AKIKO , TAKAHASHI HIDEYUKI , GOTO KAZUSHIGE 
Published: July 2019

ABSTRACT

Purpose
The present study investigated the effects of three consecutive days of endurance training under conditions of low energy availability (LEA) on the muscle glycogen content, muscle damage markers, endocrine regulation, and endurance capacity in male runners.

Methods
Seven male long-distance runners (19.9 ± 1.1 yr, 175.6 ± 4.7 cm, 61.4 ± 5.3 kg, maximal oxygen uptake [V˙O2max]: 67.5 ± 4.3 mL·kg−1·min−1) completed two trials consisting of three consecutive days of endurance training under LEA (18.9 ± 1.9 kcal·kg FFM−1·d−1) or normal energy availability (NEA) (52.9 ± 5.0 kcal·kg FFM−1·d−1). The order of the two trials was randomized, with a 2-wk interval between trials. The endurance training consisted of 75 min of treadmill running at 70% of V˙O2max. Muscle glycogen content, respiratory gas variables, and blood and urine variables were measured in the morning for three consecutive days of training (days 1–3) and on the following morning after training (day 4). As an indication of endurance capacity, time to exhaustion at 19.0 ± 0.8 km·h−1 to elicit 90% of V˙O2max was evaluated on day 4.

Results
During the training period, body weight, fat-free mass, and skeletal muscle volume were significantly reduced in LEA (P = 0.02 for body weight and skeletal muscle volume, P = 0.01 for fat-free mass). Additionally, muscle glycogen content was significantly reduced in LEA (~30%, P < 0.001), with significantly lower values than those in NEA (P < 0.001). Time to exhaustion was not significantly different between the two trials (~20 min, P = 0.39).

Conclusions
Three consecutive days of endurance training under LEA decreased muscle glycogen content with lowered body weight. However, endurance capacity was not significantly impaired.