bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Facile and Efﬁcient Approach for the Synthesis of 3-Aryl-4-hydroxy-1,3-thiazolidin-2-ones
Authors: Zhu Yue , Wang Qilin , Luo Haofan , Wang Zijuan , Zhang Guolin , Yu Yongping 
Published: April 2019

A facile and efficient method for the synthesis of 3-aryl-4-hydroxy-1,3-thiazolidin-2-ones by the reaction of 1,4-dithiane-2,5-diol with acyl azides is reported. This reaction proceeded well at 80 °C to afford products in excellent yields for a wide range of substrates. A possible mechanism has been proposed.
Evaluation of an Alternative Staining Method Using SYTO 13 to Determine Reticulated Platelets
Authors: Hille Laura , Cederqvist Marco , Hromek Julia , Stratz Christian , Trenk Dietmar , Nührenberg Thomas 
Published: April 2019

AbstractReticulated platelets reflect the rate of platelet turnover and represent the youngest circulating platelets in peripheral blood. Reticulated platelets contain residual ribonucleic acid (RNA) from megakaryocytes which is lost in a time-dependent manner and can be transcribed into proteins even in the absence of a nucleus. An increased proportion of reticulated platelets is associated with higher platelet reactivity, cardiovascular events and mortality. At present, a fully automated assay system (SYSMEX haematology analyser) is available for analysis. This method, however, is not suitable for extended laboratory investigations like subsequent cell sorting. Flow cytometry analysis after staining with thiazole orange (TO) is frequently used in such settings despite several limitations. Here, we describe a new assay for determination of reticulated platelets by flow cytometry using the nucleic acid staining dye SYTO 13 and compare it with SYSMEX and TO staining as current standards. A significant correlation between immature platelet fraction (IPF) determined by SYSMEX XE-2100 analyser and results obtained with the SYTO 13-based assay was observed (r = 0.668, p < 0.001) which was stable during a reasonable time period. In contrast, the correlation between TO staining and IPF was weaker (r = 0.478, p = 0.029) and lost after 90 minutes of staining. SYTO 13 staining of platelets enabled sorting of RNAlow and RNArich platelets which was confirmed by RNA quantification of sorted platelets. Except for fixation of platelets, sorting of these platelet sub-populations was stable under various experimental settings. In summary, determination of reticulated platelets with the new SYTO 13 assay offers distinct technical advantages enabling further laboratory processing.
Is a Chest Tube Necessary after Video-Assisted Thoracoscopic Mediastinal Tumor Resection?
Authors: Liu Yu-Wei , Chen Hao-Wei , Lee Jui-Ying , Chiang Hung-Hsing , Li Hsien-Pin , Chang Po-Chih , Chou Shah-Hwa 
Published: April 2019

Background The omission of chest tubes after thoracoscopic procedures such as sympathectomy, lung biopsy, and lung resection has proven efficacious in decreasing pain and length of hospital stay in some cases. However, its safety for mediastinal diseases remains unclear. This study evaluated the feasibility and outcome of eliminating chest drains after video-assisted thoracoscopic surgery (VATS) for mediastinal tumor resection.
Methods We retrospectively investigated 70 patients receiving VATS mediastinal tumor resection in a single institution between January 2016 and November 2018. A total of 39 patients (drain group) received postoperative chest drains and 31 patients (no-drain group) did not. Group clinical outcomes and operation data were compared. A propensity score matching analysis was further performed to yield a fairer comparison.
Results Before propensity score matching, the no-drain group had a higher prevalence of cystic lesions, a shorter operative time, and less blood loss compared with the drain group (p = 0.015, p = 0.018, and p < 0.001, respectively). After matching, the group differences in these perioperative variables lost significance (p = 0.095, 0.4, and 0.2, respectively). The no-drain group had lower postoperative day 2 pain scores and shorter postoperative hospital stays than the drain group, regardless of whether they were matched (pain: p = 0.028; hospital stay < 0.001) or not (pain: p = 0.003; hospital stay < 0.001). No major adverse events occurred in either group during hospitalization or follow-up period.
Conclusion Eliminating chest drain placement after VATS mediastinal tumor resection may benefit some patients and decrease postoperative pain and hospital stay without increasing complications or compromising patient safety.
Binding of Coagulation Factor XIII Zymogen to Activated Platelet Subpopulations: Roles of Integrin αIIbβ3 and Fibrinogen
Authors: Kotova Yana N. , Podoplelova Nadezhda A. , Obydennyy Sergey I. , Kostanova Elizaveta A. , Ryabykh Alexander A. , Demyanova Aleksandra S. , Biriukova Maria I. , Rosenfeld Mark A. , Sokolov Alexey V. , Chambost Herve , Kumskova Maria A. , Ataullakhanov Fazoil I. , Alessi Marie-Christine , Panteleev Mikhail A. 
Published: April 2019

AbstractFactor XIIIa (fXIIIa) is a transglutaminase that plays a crucial role in fibrin clot stabilization and regulation of fibrinolysis. It is known to bind to procoagulant platelets. In contrast, the zymogen fXIII interaction with platelets is not well characterized. We investigated the interaction of zymogen fXIII with activated platelet subpopulations. Confocal microscopy and flow cytometry using fluorescently labelled factors and antibodies. Phosphatidylserine (PS)-positive activated platelets bound 700 to 800 molecules/cell of fXIII at 100 nM, while both PS-negative activated platelets and resting platelets bound 200 to 400 molecules/cell. The binding was reversible, calcium-independent and linear within the fXIII concentration range of up to 1,000 nM. fXIII predominantly bound to the caps of procoagulant platelets and co-localized with fibrinogen. Exogenous fibrinogen promoted fXIII binding by activated PS-negative platelets; this effect was abolished by the integrin αIIbβ3 antagonist monafram. The fXIII binding was 1.5- to 3-fold decreased for platelets from four patients with grey platelet syndrome, and was variable for platelets from six patients with Glanzmann's thrombasthenia. Strong platelet stimulation, fibrinogen and αIIbβ3 play essential roles in fXIII binding, without any of them fXIII does not bind to platelets. The preferential binding in the cap-like structures might be important for increasing local fXIII concentration in platelet thrombi.
A Novel Frameshift Homozygous Mutation in DHCR7 with a Known Missense Homozygous Mutation in the PROC in a 6-Year-Old Boy: A Child with Two Rare Genetic Diseases
Authors: Gumus Evren 
Published: April 2019

AbstractIn the present case report, we described a 6-year-old-boy with developmental delay, mental retardation, lack of speech, skin scars, and 2 to 3 toe syndactyly from healthy consanguineous Turkish parents. The whole exome sequencing (WES) analysis of this patient showed homozygous variant c.418T > C p.(Cys140Arg) in PROC gene and novel homozygous variant c.57dupC p.(Asn20Glnfs*2) in the DHCR7 gene. This finding demonstrated that WES is of great value for the diagnosis of two separate genetic disorders in a patient with multiple dysmorphic and other clinical features. It should also be kept in mind that the coexistence of two autosomal recessive diseases could be observed in highly related consanguineous marriages. The combined evaluation of clinical and laboratory data provided extremely valuable insight into the diagnosis of this unique case.
Dresden's Musical Ruins
Authors: Sprigge Martha 
Published: April 2019

AbstractIn studies of memory politics in post-war Germany, the role that music played in responding to the Allied bombing of Dresden on 13–14 February 1945 has been overlooked. This article examines one of the first musical reactions to this traumatic event: Rudolf Mauersberger's mourning motet Wie liegt die Stadt so wüst (How Deserted Lies the City, 1945). I argue that Mauersberger, who served as cantor of the world-famous Kreuzchor from 1930 until his death in 1971, used allegory rather than testimony to formulate a response to the firebombing that resonated with historical customs familiar to the city's residents. When premièred in the bombed-out Kreuzkirche, Mauersberger's music provided a communal setting to confront the effects of the air war, transforming a space of destruction into one of contemplation and mourning. Both his compositional process and the performance transformed rubble (the material aftermath of the attack) into a ruin (an aesthetic object).
Direct measurement of ion chamber correction factors, kQ and kB, in a 7 MV MRI-linac
Authors: de Prez Leon , Woodings Simon , de Pooter Jacco , van Asselen Bram , Wolthaus Jochem , Jansen Bartel , Raaymakers Bas 
Published: April 2019

Abstract
The output of MRI-integrated photon therapy (MRgXT) devices is measured in terms of absorbed dose to water, D
w. Traditionally this is done with reference type ion chambers calibrated in a beam quality Q
0 without magnetic field. To correct the ion chamber response for the application in the magnetic field, a factor needs to be applied that corrects for both beam quality Q and the presence of the magnetic field B, k
Q,B. This can be expressed as the product of k
Q, without magnetic field, and ion chamber magnetic field correction, k
B. k
B depends on the magnetic field strength and its direction, the direction of the beam and the orientation and type of the ion chamber. In this study, for the first time, both k
Q and k
B were measured directly for six waterproof ion chambers (3  ×  PTW 30013 and 3  ×  IBA FC65-G) in a pre-clinical 7 MV MRI-linac at 0 T and at 1.5 T. Measurements were done with the only available primary standard built for this purpose, a water calorimeter. Resulting k
Q factors for PTW and IBA chambers were 0.985(5) and 0.990(4), respectively. k
B factors were measured with the chambers in antiparallel direction to the magnetic field (|| 180°), and perpendicular direction (⊥  −90°). k
B|| and k
B⊥ for the PTW chambers were 0.985(6) and 0.963(4), respectively and for IBA chambers 0.995(4) and 0.956(4). Agreement with the available literature values was shown, partly caused by the relatively large standard deviation (SD) in those values. The values in this study are currently the only available measured values for k
Q and k
B in an MRI-linac that are directly linked to the international traceability framework for the quantity absorbed dose to water, D
w.
Performance of Matching Methods as Compared With Unmatched Ordinary Least Squares Regression Under Constant Effects
Authors: Vable Anusha M , Kiang Mathew V , Glymour M Maria , Rigdon Joseph , Drabo Emmanuel F , Basu Sanjay 
Published: April 2019

AbstractMatching methods are assumed to reduce the likelihood of a biased inference compared with ordinary least squares (OLS) regression. Using simulations, we compared inferences from propensity score matching, coarsened exact matching, and unmatched covariate-adjusted OLS regression to identify which methods, in which scenarios, produced unbiased inferences at the expected type I error rate of 5%. We simulated multiple data sets and systematically varied common support, discontinuities in the exposure and/or outcome, exposure prevalence, and analytical model misspecification. Matching inferences were often biased in comparison with OLS, particularly when common support was poor; when analysis models were correctly specified and common support was poor, the type I error rate was 1.6% for propensity score matching (statistically inefficient), 18.2% for coarsened exact matching (high), and 4.8% for OLS (expected). Our results suggest that when estimates from matching and OLS are similar (i.e., confidence intervals overlap), OLS inferences are unbiased more often than matching inferences; however, when estimates from matching and OLS are dissimilar (i.e., confidence intervals do not overlap), matching inferences are unbiased more often than OLS inferences. This empirical “rule of thumb” may help applied researchers identify situations in which OLS inferences may be unbiased as compared with matching inferences.
Structural Covariance Reveals Alterations in Control and Salience Network Integrity in Chronic Schizophrenia
Authors: Spreng R Nathan , DuPre Elizabeth , Ji Jie Lisa , Yang Genevieve , Diehl Caroline , Murray John D , Pearlson Godfrey D , Anticevic Alan 
Published: April 2019

Abstract
Schizophrenia (SCZ) is recognized as a disorder of distributed brain dysconnectivity. While progress has been made delineating large-scale functional networks in SCZ, little is known about alterations in grey matter integrity of these networks. We used a multivariate approach to identify the structural covariance of the salience, default, motor, visual, fronto-parietal control, and dorsal attention networks. We derived individual scores reflecting covariance in each structural image for a given network. Seed-based multivariate analyses were conducted on structural images in a discovery (n = 90) and replication (n = 74) sample of SCZ patients and healthy controls. We first validated patterns across all networks, consistent with well-established functional connectivity reports. Next, across two SCZ samples, we found reliable and robust reductions in structural integrity of the fronto-parietal control and salience networks, but not default, dorsal attention, motor and sensory networks. Well-powered exploratory analyses failed to identify relationships with symptoms. These findings provide evidence of selective structural decline in associative networks in SCZ. Such decline may be linked with recently identified functional disturbances in associative networks, providing more sensitive multi-modal network-level probes in SCZ. Absence of symptom effects suggests that identified disturbances may underlie a trait-type marker in SCZ.
Disparities in Short-Term Depression Among Prefrontal Cortex Synapses Sustain Persistent Activity in a Balanced Network
Authors: Yoon Jae Young , Lee Hyoung Ro , Ho Won-Kyung , Lee Suk-Ho 
Published: April 2019

AbstractPersistent activity of cue-representing neurons in the prefrontal cortex (PFC) is regarded as a neural basis for working memory. The contribution of short-term synaptic plasticity (STP) at different types of synapses comprising the cortical network to persistent activity, however, remains unclear. Characterizing STP at synapses of the rat PFC layer 5 network, we found that PFC synapses exhibit distinct STP patterns according to presynaptic and postsynaptic identities. Excitatory postsynaptic currents (EPSCs) from corticopontine (Cpn) neurons were well sustained throughout continued activity, with stronger depression at synapses onto fast-spiking interneurons than those onto pyramidal cells. Inhibitory postsynaptic currents (IPSCs) were sustained at a weaker level compared with EPSC from Cpn synapses. Computational modeling of a balanced network incorporating empirically observed STP revealed that little depression at recurrent excitatory synapses, combined with stronger depression at other synapses, could provide the PFC with a unique synaptic mechanism for the generation and maintenance of persistent activity.
Adherence and Viral Suppression Among Participants of the Patient-centered Human Immunodeficiency Virus (HIV) Care Model Project: A Collaboration Between Community-based Pharmacists and HIV Clinical Providers
Authors: Byrd Kathy K , Hou John G , Bush Tim , Hazen Ron , Kirkham Heather , Delpino Ambrose , Weidle Paul J , Shankle Michael D , Camp Nasima M , Suzuki Sumihiro , Clay Patrick G ,   Patient-centered HIV Care Model Team, Aguirre Michael , Akinbosoye Osayi , Bamberger David M , Bluml Ben , Bullock Katura , Burrell Diane C , Bush Tim , Bush Clifton , Byrd Kathy K , Cadwell Chad , Camp Nasima M , Cardarelli Roberto , Clark Terri , Clay Patrick G , Crim Andrew , Cure Angela , Darin Kristin , Dean Traci , Delpino Ambrose , DeMayo Michael , Elrod Shara , Eschmann Ashley L , Farmer David , Farnan Rose , Free Heather , Gudzelak Andrew , Halbur Andrew , Hardnett Felicia , Hazen Ronald , Hilker Heidi , Hou John , Hujdich Brian , Johnson Lisa , Kirkham Heather , Lecounte James , Lio Sayuri , Lo Guanzhong , Middleton Sondra , Mills Brittany , Nguyen Christopher M , Ortiz Linda , Pietrandoni Glen , Scarsi Kimberly K , Schommer Jon , Shankle Michael D , Shrestha Ram , Smith Daron , Suzuki Sumihiro , Taitel Michael S , Teferi Gebeyehu N , Tomer Vikas , Torres Louis , Weidle Paul J , Whitfield Carmelita , Willman Jason E 
Published: April 2019

Abstract

Background
Human immunodeficiency virus (HIV) viral suppression (VS) decreases morbidity, mortality, and transmission risk.

Methods
The Patient-centered HIV Care Model integrated community-based pharmacists with HIV medical providers and required them to share patient clinical information, identify therapy-related problems, and develop therapy-related action plans.
Proportions adherent to antiretroviral therapy (proportion of days covered [PDC] ≥90%) and virally suppressed (HIV RNA &lt;200 copies/mL), before and after model implementation, were compared. Factors associated with postimplementation VS were determined using multivariable logistic regression; participant demographics, baseline viral load, and PDC were explanatory variables. PDC was modified to account for time to last viral load in the year postimplementation, and stratified as &lt;50%, 50% to &lt;80%, 80% to &lt;90%, and ≥90%.

Results
The 765 enrolled participants were 43% non-Hispanic black, 73% male, with a median age of 48 years; 421 and 649 were included in the adherence and VS analyses, respectively. Overall, proportions adherent to therapy remained unchanged. However, VS improved a relative 15% (75% to 86%, P &lt; .001). Higher PDC (adjusted odds ratio [AOR], 1.74 per 1-level increase in PDC category [95% confidence interval {CI}, 1.30–2.34]) and baseline VS (AOR, 7.69 [95% CI, 3.96–15.7]) were associated with postimplementation VS. Although non-Hispanic black persons (AOR, 0.29 [95% CI, .12–.62]) had lower odds of suppression, VS improved a relative 23% (63% to 78%, P &lt; .001).

Conclusions
Integrated care models between community-based pharmacists and primary medical providers may identify and address HIV therapy–related problems and improve VS among persons with HIV.
Influenza A Reinfection in Sequential Human Challenge: Implications for Protective Immunity and “Universal” Vaccine Development
Authors: Memoli Matthew J , Han Alison , Walters Kathie-Anne , Czajkowski Lindsay , Reed Susan , Athota Rani , Angela Rosas Luz , Cervantes-Medina Adriana , Park Jae-Keun , Morens David M , Kash John C , Taubenberger Jeffery K 
Published: April 2019

Abstract

Background
Identification of correlates of protection against human influenza A virus infection is important in development of broadly protective (“universal”) influenza vaccines. Certain assumptions underlie current vaccine developmental strategies, including that infection with a particular influenza A virus should offer long-term or lifelong protection against that strain, preventing reinfection. In this study we report observations made when 7 volunteers participated in sequential influenza challenge studies where they were challenged intranasally using the identical influenza A(H1N1)pdm09 virus approximately 1 year apart. We evaluate and describe the outcomes of these 7 rechallenge participants and discuss what these results may suggest about correlates of protection and development of more broadly protective influenza vaccines.

Methods
Seven participants were enrolled in 2 viral challenge studies at 7.5- to 18.5-month intervals. Both challenge studies used the identical lot of influenza A (H1N1)pdm09 virus administered intranasally. We evaluated pre- and postchallenge hemagglutination inhibition, neuraminidase inhibition, and stalk antibody titers; peripheral blood leukocyte host gene expression response profiles; daily viral detection via nasal wash; and clinical signs and symptoms.

Results
At least 3 of 7 participants demonstrated confirmed laboratory evidence of sequential infection, with 5 of 7 demonstrating clinical evidence.

Conclusions
The data presented in this report demonstrate that sequential infection with the identical influenza A virus can occur and suggest it may not be rare. These data raise questions about immune memory responses in an acute superficial respiratory mucosal infection and their implications in development of broadly protective influenza vaccines. Further investigation of these observations is warranted.

Clinical Trials Registration
NCT01646138; NCT01971255.
Uniportal video-assisted thoracic surgery lobectomy: a consensus report from the Uniportal VATS Interest Group (UVIG) of the European Society of Thoracic Surgeons (ESTS)
Authors: Bertolaccini Luca , Batirel Hasan , Brunelli Alessandro , Gonzalez-Rivas Diego , Ismail Mahmoud , Ucar Antonio Martin , Ng Calvin S H , Scarci Marco , Sihoe Alan D L , Ugalde Paula A , Abu Akar Firas , Bedetti Benedetta , Nadal Sergio Bolufer , Brandolini Jury , Crucitti Pierfilippo , Enyedi Attila , Fernando Hiran C , Furak Jozsef , Gallego-Poveda Javier , Galvez-Munos Carlos , Hanke Ivo , Hernandez-Arenas Luis A , Janik Miroslav , Juhos Peter , Libretti Lidia , Lucciarini Paolo , Macrì Paolo , Margaritora Stefano , Mahoozi Hamid Reza , Nachira Dania , Pardolesi Alessandro , Pischik Vadim , Sagan Dariusz , Schreurs Hermien , Sekhniaidze Dmitrii , Socci Laura , Tosi Davide , Turna Akif , Vannucci Fernando , Zielinski Marcin , Rocco Gaetano 
Published: April 2019

Abstract

OBJECTIVES
Our goal was to report the results of the first consensus paper among international experts in uniportal video-assisted thoracoscopic surgery (UniVATS) lobectomy obtained through a Delphi process, the objective of which was to define and standardize the main procedural steps, optimize its indications and perioperative management and identify elements to assist in future training.

METHODS
The 40 members of the working group were convened and organized on a voluntary basis by the Uniportal VATS Interest Group (UVIG) of the European Society of Thoracic Surgeons (ESTS). An e-consensus finding exercise using the Delphi method was applied to require 75% agreement for reaching consensus on each question. Repeated iterations of anonymous voting continued for 3 rounds.

RESULTS
Overall, 31 international experts from 18 countries completed all 3 rounds of questionnaires. Although a technical quorum was not achieved, most of the responders agreed that the maximum size of a UniVATS incision should be ≤4 cm. Agreement was reached on many points outlining the currently accepted definition of a UniVATS lobectomy, its indications and contraindications, perioperative clinical management and recommendations for training and future research directions.

CONCLUSIONS
The UVIG Consensus Report stated that UniVATS offers a valid alternative to standard VATS techniques. Only longer follow-up and randomized controlled studies will predict whether UniVATS represents a valid alternative approach to multiport VATS for major lung resections or whether it should be performed only in selected cases and by selected centres. The next step for the ESTS UVIG is the establishment of a UniVATS section inside the ESTS databases.