bluuebunny/crossref_metadata_2025_split huggingface dataset:
Estaciones automatizadas preanalíticas en el laboratorio de hemostasia. Estudio observacional descriptivo prospectivo, realizado en un hospital universitario de referencia entre el 15 de abril y 15 de julio de 2017
Authors: Sua Luz Fernanda , Amézquita María Alejandra , Hernández Daniel Esteban , Alcalá-Flores Mercedes , Leib Carl Sebastian , Aguirre-Rojas Marisol , Fernández-Trujillo Liliana 
Published: May 2019

Introducción: los errores del proceso de análisis de las muestras del laboratorio clínico, impactan negativamente a la práctica médica, la seguridad del paciente e incrementan los costos de atención en la salud. El uso de estaciones automatizadas demostró una disminución de los errores en los laboratorios clínicos de inmunoquímica y hematología. Se propone comparar las primeras estaciones automatizadas de hemostasia con el método manual para determinar los interferentes en las muestras de cinco servicios del hospital durante tres meses.Métodos: estudio observacional descriptivo prospectivo de corte trasversal. Las muestras que ingresaron al laboratorio clínico fueron analizadas por la estación automatizada y el método manual. Las interferentes bilirrubinas, hemoglobina, lipemia, volumen de llenado del tubo y obstrucción fluídica-coágulo se estudiaron con ambos métodos. Se realizó el análisis estadístico y se calculó el índice kappa para determinar la fuerza de la concordancia entre los métodos.Resultados: de 8.970 muestras analizadas, 29 % provinieron del servicio de urgencias. Las muestras aportadas por la unidad de cuidado intensivo reportaron más interferentes con ambos métodos; la estación automatizada reportó más interferentes que el método manual, con un índice kappa 0,52; la bilirrubina fue el interferente más detectado por ambos métodos. El método manual no evidenció el interferente volumen de llenado del tubo ni obstrucción fluídica-coágulo, los cuales fueron detectados por la estación automatizada.Conclusiones: la estación automatizada detectó más interferentes en comparación con el método manual. Los interferentes son errores preanalíticos en el laboratorio de hemostasia y son detectados con poca frecuencia de forma visual, especialmente los interferentes volúmenes de llenado del tubo y obstrucción fluídica-coágulo.
Literacy Engagement in Multilingual and Multicultural Learning Spaces
Authors: Kapoyannis Theodora 
Published: May 2019

This article presents qualitative findings from a larger design-based research doctoral study in which I examined the impact of a curricular innovation to challenge the monolingual and monocultural norms of literacy practices and to be responsive to the linguistic and cultural landscape of 21st century classrooms. I collaborated with 11 university preservice teachers and 28 English language learners (ELLs) in Grades 2 and 3 to design and implement a literacy intervention, called the Name Jar Project, focused on cultivating literacy engagement. I used constant comparison analysis to analyze data sources, which included field notes, student artifacts, and preservice teachers’ reflections. This article documents the positive impact of the intervention in supporting the students’ linguistic and cultural needs, affirming their identities, and bolstering their vocabulary development.
Cet article présente les constatations qualitatives d’une étude de recherche de doctorat plus importante conforme au modèle méthodologique de recherche-design (design-based research, ou DBR) dans laquelle j’ai examiné l’impact d’une innovation pédagogique conçue pour remettre en cause les normes unilingues et uniculturelles des pratiques de littératie et pour permettre à l’enseignement de s’adapter au paysage linguistique et culturel des salles de classe du 21e siècle. J’ai collaboré avec 11 enseignantes et enseignants universitaires en formation et 28 apprenantes et apprenants de la langue anglaise (English language learners,ou ELLs) de 2e et 3e année afin de concevoir et de mettre en œuvre une intervention en matière de litératie, baptisée Name Jar Project, centrée sur une émarche favorable à la culture de la littératie. J’ai constamment eu recours à des techniques d’analyse comparative pour analyser les sources de données, lesquelles comprenaient des notes, des artefacts associés au prénom de chaque élève et des réflexions d’enseignantes et enseignants en formation. Cet article documente l’impact positif de l’intervention sur la réponse aux besoins linguistiques et culturels des élèves, l’affirmation de leur identité et la stimulation du développement de leur vocabulaire.
A invisibilidade do Inativo: realidades jurídico-sociais da inatividade no Corpo de Bombeiros e Polícia Militar
Authors: De Araújo Marcos 
Published: May 2019

Este trabalho, de métodos mistos concomitantes, objetiva examinar as atuais realidades jurídico-sociais nos diferentes postos e graduações após a inatividade, no Corpo de Bombeiros e na Polícia Militar do Distrito Federal, procurando identificar como esses militares se sentem no tocante ao tratamento a eles prestados por suas respectivas corporações. Busca também aquilatar se é jurídica e socialmente oportuna, e em que grau, a inserção de programa preparativo para a inatividade como Direito Fundamental. Para garantir a precisão dos resultados, evitando distorções de análise de interpretações, o artigo se baseia em coleta de informação de 452 questionários on-line. Visando entender a natureza do fenômeno estudado, adotou-se também entrevistas abertas e a observação participante.
Simultaneous cardio-cerebral embolization associated with atrial fibrillation: a case report
Authors: Abe Soichiro , Tanaka Kanta , Yamagami Hiroshi , Sonoda Kazutaka , Hayashi Hiroya , Yoneda Shuichi , Toyoda Kazunori , Koga Masatoshi 
Published: May 2019

Abstract
Background: Simultaneous cerebral and myocardial infarction is called cardiocerebral infarction (CCI), and is rarely encountered. Because of the narrow time window and complex pathophysiology, CCI is challenging to immediately diagnose and treat.

Case presentation: A 73-year-old woman suddenly developed right hemiplegia and severe aphasia. Twelve-lead electrocardiography showed tachycardic atrial fibrillation without any significant ST-T change. Magnetic resonance imaging revealed a proximal middle cerebral artery occlusion. She was immediately treated with alteplase at the dosage approved for ischemic stroke followed by mechanical thrombectomy as bridging therapy, and complete recanalization was achieved. Aphasia improved and she began to complain of chest pain, and reported that she had experienced chest discomfort just prior to right limb weakness. Coronary angiography showed a partial filling defect in the right coronary artery with rapid and adequate distal flow, for which percutaneous coronary intervention was not required. Alteplase was suggested to have effectively resolved the coronary emboli. The occlusions of the cerebral and coronary arteries were assumed to have occurred nearly simultaneously and cardiogenic embolism due to atrial fibrillation was considered as the most likely etiology.

Conclusions: As seen in the present case, CCI may benefit from immediate treatment with intravenous tissue plasminogen activator (IV-tPA). Although which of percutaneous coronary intervention or cerebral thrombectomy should be performed first remains unclear, we must decide whether to rescue the brain or heart first in each patient within a limited window of time. This dilemma has recently become evident in this era with mechanical thrombectomy strongly established as an effective intervention for acute ischemic stroke. Close cooperation between stroke physicians and cardiologists is becoming more important.
Virtual Reality Social Prediction Improvement and Rehabilitation Intensive Training (VR-SPIRIT) for paediatric patients with congenital cerebellar diseases: study protocol of a randomized controlled trial.
Authors: Butti Niccolò , Biffi Emilia , Genova Chiara , Romaniello Romina , Redaelli Davide Felice , Reni Gianluigi , Borgatti Renato , Urgesi Cosimo 
Published: May 2019

Abstract
Background: Patients with cerebellar malformations exhibit not only movement problems, but also important deficits in social cognition. Thus, rehabilitation approaches should not only involve the recovery of motor function but also of higher-order abilities, such as processing of social stimuli. In keeping with the general role of the cerebellum in anticipating and predicting events, we used a VR-based rehabilitation system to implement a social cognition intensive training specifically tailored to improve predictive abilities in social scenarios (VR-Spirit).
Methods: The study is an interventional randomized controlled trial that aims to recruit 42 children, adolescents and young adults with congenital cerebellar malformations, randomly allocated to the experimental or active control group. The experimental group is administered the VR-Spirit, requiring the participants to compete with different avatars in the reaching of recreational equipment and implicitly prompting them to form expectations about their playing preference. The active control group participates to a VR-training with standard games currently adopted for motor rehabilitation. Both trainings are composed by 8 45-minute sessions and are administered in the GRAIL VR-Lab (Motekforce Link, NL), an integrated platform that allows patients to move in natural and attractive VR environments. An evaluation session in VR with the same paradigm used in the VR-Spirit but implemented in a different scenario is administered at the beginning (T0) and at the end (T1) of the two trainings. Moreover, a battery of neurocognitive tests spanning different domains are administered to all participants at T0, T1 and in a follow-up session after two months from the end of the two trainings (T2).
Discussion: This study offers a novel approach for rehabilitation based on specific neural mechanisms of the cerebellum. We aim to investigate the feasibility and efficacy of a new intensive social cognition training in a sample of Italian patients aged 7-25 years with congenital cerebellar malformations. We expect that VR-Spirit could enhance social prediction ability and indirectly improve cognitive performance in diverse domains. Moreover, through the comparison with a VR-active control training we aim to verify the specificity of VR-Spirit in improving social perception skills.
Fire needle acupuncture or moxibustion for chronic plaque psoriasis: study protocol for a randomized controlled trial
Authors: Chen Zhaoxia , Zhou Dongmei , Wang Yan , Lan Haibing , Duan Xingwu , Li Bohua , Zhao Jingxia , Li Wei , Liu Zhengrong , Di Tingting , Guo Xinwei , Zhang Jinchao , Li Bo , Feng Shuo , Li Ping 
Published: May 2019

Abstract
Background: Psoriasis is a chronic, immune-mediated disorder with chronic plaque psoriasis during remission stage. Patients often have a slow course and long history of the disease. The refractory type of psoriasis is a stubborn rash that does not subside easily. We designed this randomized controlled trial to compare the effectiveness and relapse rates of plaque psoriasis in patients treated with either acupuncture, moxibustion or calcipotriol ointment. The ultimate aim of the study is to select an effective traditional Chinese medicine (TCM) clinical program for patients with plaque psoriasis.
Methods: The study will be a multi-center, prospective randomized controlled trial (RCT), which compares the effectiveness of fire needle therapy, moxibustion and calcipotriol ointment. A total of 160 plaque psoriasis patients will be recruited and randomly assigned to receive either fire needle therapy (group A1), moxibustion (group A2) or calcipotriol ointment (group B). All participants will receive a 8-week treatment and then be followed up for another 24 weeks. The primary outcomes measured are relapse rates and Psoriasis area and severity index (PASI) score of the target lesions. The secondary outcomes are target lesion onset time, area and severity score. In addition, the overall clinical cure and improvement rate, dermatology life quality index (DLQI), TCM syndrome score, target recurrence rate and interval of the target lesion will be measured.
Discussion: This study is to determine whether fire needle therapy or moxibustion could improve the clinical effectiveness of psoriasis lesions and reduce the relapse rate. Once completed, it will provide information regarding therapeutic evaluation on fire needle therapy or moxibustion for plaque psoriasis, which will assist clinicians select the most effective treatment options for patients.
Trial registration: ChiCTR1800019588; Pre-results; Registered on 19 November 2018; International Clinical Trials Registry Platform (ICTRP). URL: http://apps.who.int/trialsearch/Trial2.aspx?TrialID=ChiCTR1800019588
Keywords: Fire needle therapy, Moxibustion, Plaque Psoriasis, Clinical effectiveness, Relapse rate
Multivariable prediction model for predicting deaths in severe dengue cases
Authors: S Saiful Safuan Md , Zaid Masliza , Leong Kar Nim , Hawari Rossman , Jelani Anilawati Mat , Abd-Rahman Marzilawati , Ooh Ping Yee , Shanmugarajoo Anusha , Kumar Suresh , Mustafa Mahiran 
Published: May 2019

Abstract
Background

Many predictive models have been developed to predict an outbreak, identify and stratify dengue but none in predicting mortality in severe dengue cases. To build a predictive model for deaths in severe dengue, a multicentre retrospective cohort study was conducted.

Methods

Patients with severe dengue based on WHO 2009 classification were studied. Demographic, clinical and laboratory data were collected at diagnosis of severe dengue. Penalised regression was used for variable selection and model-building. Ten-fold cross-validation with 1000 repeats was performed for internal validation.

Results

A cohort of 786 severe dengue cases including 35 deaths was analysed. Our model that predicts death in severe dengue cases comprises eight independent predictors: persistent diarrhoea, BMI, respiratory rate, platelet count, AST, serum bicarbonate, serum lactate and serum albumin. The AUROC is 89·6% with a sensitivity of 99·6%, specificity of 23·6%, positive predictive value of 96·6%, negative predictive value of 71·1%, positive likelihood ratio 1·45 and negative likelihood ratio 0·01. We also found that the proportion of patients that were in the febrile phase at diagnosis of severe dengue for the overall cohort, decompensated and compensated shock were 74·3%, 73% and 75·4%, respectively.

Conclusions

We developed a high performance dengue mortality prediction model comprising clinical and laboratory data and deployed an open access web-based tool (www.saifulsafuan.com/REPROSED2017E2) for any centre to utilise for local validation and found that a large majority of patients developed severe dengue during febrile phase.
MicroRNA expression profiling of peripheral blood mononuclear cells associated with syphilis
Authors: Huang Tao , Zhang Jun , Ke Wujian , Zhang Xiaohui , Chen Wentao , Yang Jieyi , Liao Yiwen , Liang Fangwen , Mei Shuqing , Li Mingjiu , Luo Zhenzhou , Zhang Qiwei , Yang Bin , Zheng Heping 
Published: May 2019

Abstract
Background

Treponema pallidum (T. pallidum Tp) infection evokes vigorous immune responses, resulting in tissue damage. The immune mechanism after Treponema pallidum infection is still not clear. MicroRNAs (miRNAs) have been shown, however, to influence immune cell function and consequently the generation of antibody responses during other microbe infections, but these values are unknown for Tp.

Methods

In this study, we performed a comprehensive analysis of differentially expressed miRNAs in healthy persons, untreated patients with syphilis, patients in the serofast state, and serologically cured patients. MiRNAs were profiled from patient peripheral blood obtained at the time of serological diagnosis. Then both the target sequence analysis on these different miRNAs and pathway analysis were performed to identify important immune and cell signaling pathways. Quantitative RT-PCR for analysis of microRNA

Results

There were 89 differentially regulated miRNA identified in total. Following RT-qPCR confirmation, three miRNAs (hsa-miR-195-5p, hsa-miR-223-3p, hsa-miR-589-3p) showed significant difference among serofast state, and serological cure (P<0.05). A miRNAs (hsa-miR-195-5p) showed significant differences among untreated patients and healthy individuals.

Conclusions

This is the first study of miRNA expression difference in PBMCs in different stages of T. pallium infection. Our study suggests that the combination of three miRNAs has great potential to serve as non-invasive biomarkers of Treponema pallidum infections, which will facilitate better diagnosis and treat of T. pallium infections.
Revising the BFIHOST catchment descriptor to improve UK flood frequency estimates
Authors: Griffin Adam , Young Andy , Stewart Lisa 
Published: May 2019

Abstract
The estimate of the base flow index (BFI) based on the Hydrology of Soil Types (HOST) classification, BFIHOST, provides a measure of catchment responsiveness. BFIHOST is used with other variables to estimate the median annual maximum flood (QMED) in the UK standard Flood Estimation Handbook (FEH) statistical method and is also an explanatory variable in ReFH2, the FEH design hydrograph package. The current estimates of BFIHOST are derived from a restricted linear model, and a number of issues in the catchment dataset have been identified since the original work in 1995. The BFI calculated through base flow separation tends to be underestimated in clay-dominated catchments, and the calculation technique performs poorly in ephemeral catchments or those with missing data. The pragmatic bounding of BFI coefficients for permeable soils overlying aquifer outcrops is also problematic for small catchments. This paper investigates alternative regression methods to improve base flow estimates using the HOST class data for 991 stations (compared to 575 in the original); beta regression was found to give the best performance. Combining multiple rare classes into single classes is also shown to improve performance. The new version of BFIHOST was applied to the QMED equation, showing improved performance.
A Clinical Decision Support System for Assessing the Risk of Cardiovascular Diseases in Diabetic Hemodialysis Patients
Authors: Azad Boni Tahere Talebi , Ayatollahi Haleh , Langarizadeh Mostafa 
Published: May 2019

Background:
One of the greatest challenges in the field of medicine is the increasing burden
of chronic diseases, such as diabetes. Diabetes may cause several complications, such as kidney failure
which is followed by hemodialysis and an increasing risk of cardiovascular diseases.

Objective:
The purpose of this research was to develop a clinical decision support system for assessing
the risk of cardiovascular diseases in diabetic patients undergoing hemodialysis by using a fuzzy logic
approach.

Methods:
This study was conducted in 2018. Initially, the views of physicians on the importance of assessment
parameters were determined by using a questionnaire. The face and content validity of the
questionnaire was approved by the experts in the field of medicine. The reliability of the questionnaire
was calculated by using the test-retest method (r = 0.89). This system was designed and implemented
by using MATLAB software. Then, it was evaluated by using the medical records of diabetic patients
undergoing hemodialysis (n=208).

Results:
According to the physicians' point of view, the most important parameters for assessing the
risk of cardiovascular diseases were glomerular filtration, duration of diabetes, age, blood pressure,
type of diabetes, body mass index, smoking, and C reactive protein. The system was designed and the
evaluation results showed that the values of sensitivity, accuracy, and validity were 85%, 92% and
90%, respectively. The K-value was 0.62.

Conclusion:
The results of the system were largely similar to the patients’ records and showed that the
designed system can be used to help physicians to assess the risk of cardiovascular diseases and to improve
the quality of care services for diabetic patients undergoing hemodialysis. By predicting the risk
of the disease and classifying patients in different risk groups, it is possible to provide them with better
care plans.
Phospholipase A2 is an Inflammatory Predictor in Cardiovascular Diseases: Is there any Spacious Room to Prove the Causation?
Authors: Santoso Anwar , Heriansyah Teuku , Rohman Mohammad S. 
Published: May 2019

:Lipoprotein-associated phospholipase A2 (Lp-PLA2) is an enzyme family of phospholipase A2 produced by the inflammatory cell in atherosclerotic plaque. It is transported in the circulation, attached mainly to low-density lipoprotein-cholesterol (LDL-C). It hydrolyzes glycerophospholipids particularly fatty acids at the sn-2 position and produces numerous bioactive lipids; and leads to endothelial dysfunction, atherosclerotic plaque inflammation, and development of the necrotic core in plaques.:There are two kinds of phospholipase A2, namely: secretory phospholipase A2 (sPLA2) and Lp- PLA2. They are deemed as evolving predictors of cardiovascular disease (CVD) risk in hospitaland population-based studies, including healthy subjects, acute coronary syndromes (ACS) and patients with CVD. Unfortunately, Lp-PLA2 inhibitor (darapladib) and s-PLA2 inhibitor (varespladib methyl) failed to prove to lower the risk of composite CVD mortality, myocardial infarction and stroke in those with stable CVD and ACS.:Herein, we describe the explanation based on the existing data why there is still a discrepancy among them. So, it highlights the opinion that phospholipase A2 is merely the inflammatory biomarkers of CVD and playing an important role in atherosclerosis. Further, there is more spacious room to prove the causation.
Novel p-Functionalized Chromen-4-on-3-yl Chalcones Bearing Astonishing Boronic Acid Moiety as MDM2 Inhibitor: Synthesis, Cytotoxic Evaluation and Simulation Studies
Authors: Bhatia Richa K. , Singh Lakhwinder , Garg Ruchika , Kaur Maninder , Yadav Manmohan , Madan Jitender , Kancherla Satyavathi , Pissurlenkar Raghuvir R.S. , Coutinho Evans C. 
Published: May 2019

Background:
Novel 4-[3-(6/7/8-Substituted 4-Oxo-4H-chromen-3-yl)acryloyl]phenylboronic
acid derivatives (5a-h) as well as other 6/7/8-substituted-3-(3-oxo-3-(4-substitutedphenyl)
prop-1-enyl)-4H-chromen-4-one derivatives (3a-u) have been designed as p53-MDM2
pathway inhibitors and reported to possess significant cytotoxic properties against several cancer
cell lines.

Objectives:
The current project aims to frame the structure-anticancer activity relationship of
chromen-4-on-3-yl chalcones (3a-u/5a-h). In addition, docking studies were performed on these
chromeno-chalcones in order to have an insight into their interaction possibilities with MDM2 protein.

Methods:
Twenty-nine chromen-4-on-3-yl chalcone derivatives (3a-u/5a-h) were prepared by utilizing
silica supported-HClO4 (green route with magnificent yield) and tested against four cancer
cell lines (HCT116, MCF-7, THP-1, NCIH322).

Results:
Among the series 3a-u, compound 3b exhibited the highest anticancer activity (with IC50
values ranging from 8.6 to 28.4 µM) overall against tested cancer cell lines. Interestingly, para-
Boronic acid derivative (5b) showed selective inhibition against colon cancer cell line, HCT-116
with an IC50 value of 2.35 µM. Besides the emblematic hydrophobic interactions of MDM2 inhibitors,
derivative 5b was found to exhibit extra hydrogen bonding with GLN59 and GLN72 residues
of MDM2 in molecular dynamics (MD) simulation. All the compounds were virtually nontoxic
against normal fibroblast cells.

Conclusion:
Novel compounds were obtained with good anticancer activity especially 6-
Chlorochromen-4-one substituted boronic acid derivative 5b. The molecular docking study proposed
good activity as a MDM-2 inhibitor suggesting hydrophobic as well as hydrogen bonding
interactions with MDM2.