bluuebunny/crossref_metadata_2025_split huggingface dataset:
Egg-based influenza split virus vaccine with monoglycosylation induces cross-strain protection against influenza virus infections
Authors: Tseng Yung-Chieh , Wu Chia-Yu , Liu Ming-Liang , Chen Ting-Hua , Chiang Wan-Ling , Yu Yueh-Hsiang , Jan Jia-Tsrong , Lin Kuo-I , Wong Chi-Huey , Ma Che 
Published: February 2019

Significance
Glycosylation is important for the proper folding and function of glycoproteins. However, glycosylation on viral surface protein may mask the conserved epitopes and causes poor host immune responses and protection against viral infection. To expose more antigenic sites shielded by glycans, we developed a strategy to remove the glycans on the viral surface by modification of the traditional egg-based influenza vaccine production procedure. By means of immunization with the monoglycosylated split virus vaccine, mice were induced with broader immune responses, and thus better protection against cross-strain H1N1 virus infections. The results showed that the monoglycosylated split virus vaccine is a broadly protective vaccine and can be produced through simple modifications of the current vaccine manufacturing process.
Amyloid fibril-directed synthesis of silica core–shell nanofilaments, gels, and aerogels
Authors: Cao Yiping , Bolisetty Sreenath , Wolfisberg Gianna , Adamcik Jozef , Mezzenga Raffaele 
Published: February 2019

Significance
Proteinaceous fibrils networks are ubiquitous in nature and span most domains of living matter. Among these, amyloid networks bear a twofold significance, due to their implication in the formation of amyloid plaques and tangles in vivo, but also the remarkable width of applications in which they start to emerge when synthesized in vitro. This work provides a solid strategy to improve the mechanical properties of amyloid networks by biosilicification and demonstrates how the resulting hydrogels can be used for further material design, such as in the fabrication of ultra-high porous aerogels. This contribution also advances our fundamental understanding of complex dynamic networks by introducing a general theoretical model capable of quantitatively capturing the effects of filament bundling on gel rigidity.
Synaptic retinoic acid receptor signaling mediates mTOR-dependent metaplasticity that controls hippocampal learning
Authors: Hsu Yu-Tien , Li Jie , Wu Dick , Südhof Thomas C. , Chen Lu 
Published: February 2019

Significance
Homeostatic synaptic plasticity has long been considered an anti-Hebbian mechanism that maintains the brain’s network stability, prevents aberrant Hebbian plasticity, and sustains normal cognitive functions. However, evidence supporting this hypothesis is largely lacking. Using region-specific deletions of the retinoic acid receptor RARα, a key mediator of homeostatic synaptic plasticity, we show that experience of an enriched environment (EE) engages homeostatic synaptic plasticity in hippocampal neurons in an RARα-dependent manner. EE experience in mice with deletion of RARα in the hippocampus caused runaway long-term potentiation (LTP), enhanced contextual memory, and reduced cognitive flexibility by hyperactivating mammalian target of rapamycin (mTOR) signaling. Treatment with the mTOR inhibitor rapamycin during an EE experience reversed both runaway LTP and enhanced learning, demonstrating a close functional interaction between Hebbian and homeostatic plasticity.
Anti-BCMA immunotoxins produce durable complete remissions in two mouse myeloma models
Authors: Shancer Zoë , Liu Xiu-fen , Nagata Satoshi , Zhou Qi , Bera Tapan K. , Pastan Ira 
Published: February 2019

Significance
Multiple myeloma is a deadly disease for which new therapies are urgently needed. B cell maturation antigen (BCMA) is an excellent target for therapy of myeloma because it is a lineage restricted differentiation protein that is expressed on myeloma cells and plasma cells but not on essential organs. We have produced recombinant immunotoxins that target and kill myeloma cells expressing BCMA. We have examined two mouse models of myeloma and show that the anti-BCMA immunotoxins produce long-term complete regressions in both models. These data support further preclinical development of these agents.
Calcium‐dependent, non‐apoptotic, large plasma membrane bleb formation in physiologically stimulated mast cells and basophils
Authors: Jansen C. , Tobita C , Umemoto E. U. , Starkus J. , Rysavy N. M. , Shimoda L. M. N. , Sung C. , Stokes A.J. , Turner H 
Published: February 2019

ABSTRACTLarge membrane derangements in the form of non‐detaching blebs or membrane protrusions occur in a variety of cell stress and physiological situations and do not always reflect apoptotic processes. They have been studied in model mast cells under conditions of cell stress, but their potential physiological relevance to mast cell function and formation in primary mast cells or basophils have not been addressed. In the current study, we examine the large, non‐detaching, non‐apoptotic, membrane structures that form in model and primary mast cells under conditions of stimulation that are relevant to allergy, atopy and Type IV delayed hypersensitivity reactions. We characterized the inflation kinetics, dependency of formation upon external free calcium and striking geometric consistency of formation for large plasma membrane blebs (LPMBs). We describe that immunologically stimulated LPMBs in mast cells are constrained to form in locations where dissociation of the membrane‐associated cytoskeleton occurs. Mast cell LPMBs decorate with wheat germ agglutinin, suggesting that they contain plasma membrane (PM) lectins. Electrophysiological capacitance measurements support a model where LPMBs are not being formed from internal membranes newly fused into the PM, but rather arise from stretching of the existing membrane, or inflation and smoothing of a micro‐ruffled PM. This study provides new insights into the physiological manifestations of LPMB in response to immunologically relevant stimuli and in the absence of cell stress, death or apoptotic pathways.
Pitfalls associated with lipophilic fluorophore staining of extracellular vesicles for uptake studies
Authors: Simonsen Jens B. 
Published: February 2019

ABSTRACTPost‐staining of extracellular vesicles (EVs) with lipid‐anchored fluorophores (LAFs) such as PKH67 is a widely used strategy for studying EVs but it is associated with several pitfalls. The pitfalls discussed in this commentary are related to LAF labelling of non‐EV species due to (1) lipoprotein contamination in EV samples, (2) desorption of the LAF reporters from vesicles into proteins and lipoproteins in blood and serum, and (3) the capability of the amphiphilic LAF compounds to form EV‐like particles. Awareness of these challenges and developing solutions to overcome these are important to ensure that we make relevant interpretations when using LAFs to track EVs.
Outcome-Based Critical Result Thresholds in the Adult Patient Population
Authors: Tan Eng Hooi , Yang Zhutian , Li Yingda , Metz Michael P , Loh Tze Ping 
Published: February 2019

AbstractObjectivesTo derive outcome-based critical result thresholds in the adult patient population.MethodsWe extracted deidentified laboratory results and outcomes (death or discharged) of patients 18 years and older from the Medical Information Mart for Intensive Care database. The lower and upper critical result thresholds were obtained from the nearest minimum and maximum laboratory values, which corresponded to predicted probability of death at 90%.ResultsThe critical value thresholds were sodium (<123, >153 mmol/L), potassium (<2.2, >6.6 mmol/L), bicarbonate (<15, >49 mmol/L), chloride (<82, >121 mmol/L), urea (>20 mmol/L), creatinine (>1,052 μmol/L), glucose (<1.5, >23.8 mmol/L), total calcium (<1.62, >2.95 mmol/L), magnesium (<0.37, >1.48 mmol/L), phosphate (<0.19, >2.52 mmol/L), pH (<7.22, >7.57), lactate (>5.0 mmol/L), hemoglobin (<4.6 g/dL), WBCs (>32 × 103/μL), prothrombin time (>90 seconds), and international normalized ratio (>10).ConclusionsThe indirect approach described in this study is a pragmatic way to obtain threshold values that are clinically and operationally meaningful.
Patient-Reported Outcome Measures for Breast Implant Surgery: A Pilot Study
Authors: Ng Sze , Pusic Andrea , Parker Emily , Vishwanath, Swarna , Cooter Rodney D , Elder Elisabeth , Moore Colin , McNeil John , Hopper Ingrid 
Published: February 2019

Abstract

Background
The Breast-Q Implant Surveillance module (BREAST-Q IS) is a patient-reported outcome measure (PROM) that asks 5 questions on satisfaction (shape, feel, and rippling) and symptoms (pain and tightness) derived from the BREAST-Q.

Objectives
We aimed to pilot BREAST-Q IS on patients within the Australian Breast Device Registry (ABDR), an opt-out clinical quality device registry, and explored Short Message Service (SMS) communication as a follow-up method.

Methods
Patients with a breast device surgery in the previous 10 to 15 months, age ≥18 years, with a mobile phone number, were invited to complete the 5-question PROM via SMS initially, followed by 3 phone call attempts if no response, an e-mail, and then a letter by post as a final engagement strategy.

Results
The study included 197 participants [breast augmentation (BA) = 118; breast reconstruction (BR) = 79]. Mean ± SD age was 40 ± 12 years (BA) and 44 ± 11 years (BR). Mean ± SD time since surgery was 414 ± 36 days (BA) and 413 ± 51 days (BR). The total response rate, including opt-outs, was 76%. Responses indicated that &gt;90% of BA and &gt;79% of BR were very or somewhat satisfied with shape, feel, and wrinkling; &gt;70% of BA and &gt;46% of BR reported no pain or tightness. Completion of survey via SMS was 51% (BA) and 55% (BR). Further responses were received by phone (25%, 26%), post (21%, 16%), and e-mail (3%, 3%).

Conclusions
This pilot demonstrated high levels of satisfaction and low levels of pain and tightness in patients with breast augmentation and breast reconstruction 1 year postoperatively. It also showed the effectiveness of our engagement strategy, which achieved a 76% response rate. Over 50% of respondents used SMS to reply to a 5-question PROM assessing long-term surgical outcomes. This engagement strategy will be used as BREAST-Q IS is rolled out nationally.
Proposed Guide to Lip Treatment in Caucasian Women Using Objective and Measurable Parameters
Authors: Sito Giuseppe , Consolini Luana , Trévidic Patrick 
Published: February 2019

Abstract

Background
The lips are a focal point of the face; however, their aesthetic proportions and effect on perceived facial beauty remain poorly defined. Perioral aging is highly individual, with several distinct and often simultaneous processes contributing to changes in lip contour and surface. These processes can affect lip volume and length, shape, and the degree of vermilion inversion.

Objectives
We aimed to develop a treatment guide for Caucasian women that combined a complete analysis of the lips (including the effects of aging) with consideration of the rheological characteristics of the products used to assist practitioners in tailoring rejuvenation treatment to individual patients.

Methods
We reviewed existing literature to analyze the parameters that make the lips of Caucasian women “attractive” and investigated the rheological characteristics of different hyaluronic fillers to provide guidance on the optimal treatment for each woman.

Results
Our numerical definition of the lips is based on 4 specific parameters: philtrum height, upper vermillion height, ratio between philtrum and upper vermillion height, and golden ratio between upper and lower vermillion height. In our opinion, conserving the length of hyaluronic acid chains results in dynamic fillers that may provide better results with lower risk of asymmetry compared with conventional products.

Conclusions
This new classification and associated treatment guideline aims to allow accurate assessment and enable practitioners to customize treatment for individual patients.
Bone-anchored maxillary protraction in unilateral cleft lip and palate: a cephalometric appraisal
Authors: Faco Renato , Yatabe Marilia , Cevidanes Lucia H S , Timmerman Hilde , De Clerck Hugo J , Garib Daniela 
Published: February 2019

Abstract

Objectives
The aim of this study was to evaluate the cephalometric outcome of bone-
anchored maxillary protraction (BAMP) in individuals with unilateral complete cleft lip and palate (UCLP).

Material and methods
The experimental group (EG) comprised 23 individuals (17 males and 6 females) with UCLP and a mean age of 11.7 years. At least 6 months after secondary alveolar bone grafting, Bollard miniplates were installed in the posterior region of the maxilla and in the anterior region of the mandible. Class III elastics were recommended to be worn for 24 hours/day for a mean time of 18 months. Cone beam computed tomography (CBCT) was obtained before (T1) and after treatment (T2). The control group (CG) consisted of 23 individuals with UCLP matched by initial age and gender with the EG and without any orthopaedic or surgical intervention performed between T1 and T2. The interval between T1 and T2 observations was 18 months for both groups. Twenty-one cephalometric variables were analysed. Intra- and intergroup comparisons were performed using paired and independent t-tests, respectively (P &lt; 0.05).

Results
BAMP caused a greater maxillary protrusion (SNA) and a greater decrease of Class III maxillomandibular discrepancy (ANB and Wits appraisal) compared with the CG. BAMP also caused a counterclockwise rotation of the occlusal plane (Occ Plane to FH) and an improvement in the molar relationship compared with controls.

Conclusions
BAMP therapy demonstrated a significant orthopaedic maxillary protraction and an improvement in the Class III skeletal pattern in UCLP.
Evaluation of an education program based on the theory of planned behavior for salt intake in individuals at risk of hypertension
Authors: Rahimdel T , Morowatisharifabad M A , Salehi-Abargouei A , Mirzaei M , Fallahzadeh H 
Published: February 2019

Abstract
This study was conducted to evaluate an education program based on the theory of planned behavior (TPB) for salt intake in individuals at risk of hypertension. This randomized controlled trial was conducted from February 2017 to December 2017 in Shahediyeh, Yazd Province, Iran. For this purpose, 140 people were selected and assigned to two groups of 70 each, namely, intervention and control. Data were gathered by a self-administered TPB based questionnaire, and also 24-h urinary sodium and potassium levels and systolic and diastolic blood pressure were measured. The intervention group received TPB based on a training package, while the control group received no intervention. Post-test was administered 2 months after completion of the intervention. Independent and paired t-tests, χ2 and analysis of covariance (ANCOVA) were used for data analysis. All mean scores on TPB constructs increased significantly except motivation to comply and power of control for intervention group. Average salt intake decreased in intervention group compared with the control group (−4.73 g/day �0.73 versus −0.24 g/day �0.94, P &lt; 0.001), but systolic and diastolic blood pressure did not change significantly in intervention group compared with control group. TPB can be implemented along with other approaches in educational programs to reduce salt intake. Code: IRCT201701108803N3.
From Their Perspective: The Connection between Life Stressors and Health Care Service Use Patterns of Homeless Frequent Users of the Emergency Department
Authors: Moore Megan , Conrick Kelsey M , Reddy Ashok , Allen Ann , Jaffe Craig 
Published: February 2019

Abstract
The perspective of homeless adults on their health care service utilization is not well studied. This article describes a study that used in-depth, semistructured interviews with 18 individuals to highlight the viewpoints of homeless people who are frequent users of the emergency department (ED) about the influence of life events on service utilization. Participants reported high levels of pain and comorbid psychiatric, substance use, and medical conditions. They also reported an identifiable pattern of health care utilization, often centered on a crisis event, influenced by high perceived medical needs, inability to cope after crisis, predisposing vulnerability from social determinants of health, and health care system factors. A social work case management intervention often led to a period of stability and use of ED alternatives. Modifiable targets for intervention at the health care system and local levels include improving trust and convenience of ED alternatives, enhancing consistency of care at ED-alternative sites, and educating those at risk of frequent ED use about community alternatives.
Gender equity in mass drug administration for neglected tropical diseases: data from 16 countries
Authors: Cohn Daniel A , Kelly Maureen P , Bhandari Kalpana , Zoerhoff Kathryn L , Batcho Wilfrid E , Drabo François , Negussu Nebiyu , Marfo Benjamin , Goepogui André , Lemoine Jean-Frantz , Ganefa Sitti , Massangaie Marilia , Rimal Pradip , Gnandou Issa , Anagbogu Ifeoma N , Ndiaye Mamadou , Bah Yakuba Madina , Mwingira Upendo J , Awoussi Marcel S , Tukahebwa Edridah M , Stelmach Rachel D , Mingkwan Pia C , Pou Bolivar , Koroma Joseph B , Rotondo Lisa A , Kraemer John D , Baker Margaret C 
Published: February 2019

AbstractBackgroundGender equity in global health is a target of the Sustainable Development Goals and a requirement of just societies. Substantial progress has been made towards control and elimination of neglected tropical diseases (NTDs) via mass drug administration (MDA). However, little is known about whether MDA coverage is equitable. This study assesses the availability of gender-disaggregated data and whether systematic gender differences in MDA coverage exist.MethodsCoverage data were analyzed for 4784 district-years in 16 countries from 2012 through 2016. The percentage of districts reporting gender-disaggregated data was calculated and male–female coverage compared.ResultsReporting of gender-disaggregated coverage data improved from 32% of districts in 2012 to 90% in 2016. In 2016, median female coverage was 85.5% compared with 79.3% for males. Female coverage was higher than male coverage for all diseases. However, within-country differences exist, with 64 (3.3%) districts reporting male coverage >10 percentage points higher than female coverage.ConclusionsReporting of gender-disaggregated data is feasible. And NTD programs consistently achieve at least equal levels of coverage for women. Understanding gendered barriers to MDA for men and women remains a priority.
No Significant Changes to Residual Viremia After Switch to Dolutegravir and Lamivudine in a Randomized Trial
Authors: Li Jonathan Z , Sax Paul E , Marconi Vincent C , Fajnzylber Jesse , Berzins Baiba , Nyaku Amesika N , Fichtenbaum Carl J , Wilkin Timothy , Benson Constance A , Koletar Susan L , Lorenzo-Redondo Ramon , Taiwo Babafemi O 
Published: February 2019

Abstract
In the ASPIRE trial, antiretroviral therapy (ART) switch to dolutegravir plus lamivudine (DTG+3TC) was comparable to 3-drug ART in maintaining viral suppression by standard viral load assays. We used an ultrasensitive assay to assess whether this switch led to increased residual viremia. At entry, levels of residual viremia did not differ significantly between arms (DTG+3TC vs 3-drug ART: mean, 5.0 vs 4.2 HIV-1 RNA copies/mL; P = .64). After randomization, no significant between-group differences were found at either week 24 or 48. These results show no evidence for increased viral replication on DTG+3TC and support its further investigation as a dual ART strategy.
Biomedical, psychological, environmental and behavioural factors associated with adult obesity in a nationally representative sample
Authors: Cheng Helen , Montgomery Scott , Green Andy , Furnham Adrian 
Published: February 2019

Abstract

Objective
To identify personality, biomedical and behavioural factors associated with adult obesity in a large longitudinal sample.

Method
In total, 5360 participants with data on personality, neurological functioning, maternal smoking during pregnancy, education and occupation, physical exercise, adult self-reported BMI and obesity were included in the study. Obesity at 55 years was the outcome variable.

Results
The rates of obesity increased from 9.5 to 22.8% from age 33 to 55 years. Logistic regression analyses (adjusted estimates) showed that childhood neurological functioning (OR = 1.32: 1.07–1.63, P &lt; 0.01), maternal smoking during pregnancy (OR = 1.42: 1.22–1.65, P &lt; 0.001), educational qualifications (OR = 0.54: 0.37–0.79, P &lt; 0.01), trait conscientiousness (OR = 0.80:0.74–0.86, P &lt; 0.001) and physical exercise (OR = 0.87: 0.82–0.92, P &lt; 0.001) were significant predictors of obesity at age 55 years for both men and women. Trait extraversion for men (OR = 1.16: 1.07–1.26, P &lt; 0.001) and trait emotional stability for women (OR = 0.90: 0.82–0.99, P &lt; 0.05) were also significant predictors of the outcome variable.

Conclusion
Biomedical, psychological, environmental and behavioural factors were all associated with adult obesity.
Ask the Midwives: A Hebrew Manual on Midwifery from Medieval Germany†
Authors: Baumgarten Elisheva 
Published: February 2019

Summary
This article focuses on a chapter in a manual on circumcision written in Worms in the thirteenth century by Jacob and Gershom haGozrim (the circumcisers). The third chapter of the manual contains medical instruction on how to attend to women in labour and other gynaecological conditions. Whereas the first two chapters of the manual were published in the late nineteenth century, the midwifery chapter has only been recently examined. This article is comprised of a translation of the midwifery text(s) along with an introduction to the text and the community practices it reflects. It outlines the cooperation between medical practitioners, male and female, Jewish and Christian, and discusses the medical remedies recommended and some practices current in thirteenth-century Germany.
Association of Functional Variants and Protein-to-Protein Physical Interactions of Human MutY homolog linked with Familial Adenomatous Polyposis and colorectal cancer Syndrome
Authors: Abduljaleel Zainularifeen 
Published: February 2019

AbstractThe human geneMUTYHcodes for a DNA glycosylase involved in the repair of oxidative DNA damage. Faulty MUTYH protein activity causes the accumulation of G→T transversions due to unrepaired 8-oxoG:A mismatches.MUTYHgerm-line mutations in humans are linked with a recessive form of Familial Adenomatous Polyposis (FAP) and colorectal cancer predisposition. We studied the repair capacity of variants identified in MUTYH-associated polyposis (MAP) patients. MAP is inherited in an autosomal recessive type due to mutations in MUTYH (Y165C, G382D, P54S, A22V, Q63R, G45D, S136P and N43S), indicating that both copies of the gene become inactivated. However, the parents of an individual with an autosomal recessive condition may serve as carriers, each harboring one copy of the mutated gene without showing signs or symptoms of MAP. Six protein partners have been associated with MUTYH, four via direct physical interactions, namely, hMSH6, hPCNA, hRPA1, and hAPEX1. We examined, for the first time, specific interactions of these protein partners with MAP-associated MUTYH mutants using molecular dynamics simulations. The approach provided tools for exploration of the conformational energy landscape accessible to protein partners. The investigation also determined the impact before and after energy minimization of protein-protein interactions and binding affinities of MUTYH wild type and mutant forms, as well as the interactions with other proteins. Taken together, this study provided new insights into the role of MUTYH and its interacting proteins in MAP.