bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptional antitermination integrates the expression of loci of diverse phage origin in the chimericBartonellaGene Transfer Agent BaGTA
Authors: Korotaev Aleksandr , Niggli Quirin , Dehio Christoph 
Published: November 2024

AbstractGene Transfer Agents (GTAs) are mobile genetic elements derived from bacteriophages that mediate genome-wide horizontal gene transfer (HGT) in diverse groups of prokaryotes. BaGTA, encoded by all the pathogens of the genusBartonella, is a chimeric GTA that evolved by the domestication of two phages. The run-off-replication module ROR of one phage is integrated with the capsid production, DNA packaging and lysis machinery Bgt of a second phage. Restricted to a self-sacrifying subset of the bacterial population, the position-specific DNA amplification and packaging of a genomic plasticity region enriched for genes involved in host interaction and adaptation selectively enhances the HGT frequency of these pathogenicity genes. This feature of BaGTA is considered a key innovation underlying the evolutionary success ofBartonella. Little is known, however, about the mechanism mediating the coordinated expression of therorandbgtloci. Here, we established the regulatory hierarchy, withroracting upstream of the capsid gene clusterbgtA-K. BrrG, encoded by therorlocus, controls the transcription of thebgtA-Koperon by functioning as a processive antiterminator. This study provides the first insights into the mechanism controlling the coordinated expression of the two BaGTA modules of divergent phage origin. Beyond BaGTA, we propose that antitermination is a broadly relevant mechanism for controlling HGT by GTAs of the Alphaproteobacteria.
A sequential event-responsive fluorescent reporter for inducible Cre and Flp combinatorial recombination
Authors: Pessina Giada , Camera Mattia , Loiacono Fabrizio , Uccelli Antonio , Benfenati Fabio , Medini Paolo , Trovato Francesco , Sato Sebastian Sulis 
Published: November 2024

AbstractCurrent technologies for precise genetic manipulation of cells often utilize site-specific recombinases, enabling the creation of conditional transgenic models for studying gene functions in specific tissues and at defined time windows. The advent of drug-inducible recombinases has further enhanced this field by allowing temporal control of gene expression. However, detecting gene expression patterns when multiple recombinases are used is challenging, particularly when visualizing combinatorial maps and their temporal profile. To address this, we developed Rubik, a fluorescent genetic reporter specifically designed to monitor the action of Cre and Flp recombinases by expressing different fluorophores depending on the temporal sequence of recombination events. Rubik offers four alternative fluorescent configurations: blue for Cre recombination, green for Flp recombination, yellow for Cre followed by Flp recombination, and red for Flp followed by Cre recombination. Additionally, Rubik integrates light-gated ion channels, allowing optogenetic excitation or inhibition of electrically-excitable cells based on the temporal sequence of recombination events. Moreover, to add temporal control over Rubik recombination, we optimized a trimethoprim-inducible FlpO recombinase (FlpO-DD) to use in combination with the tamoxifen-inducible Cre recombinase (ERT2CreERT2). We validated Rubik by generating a stable knock-in HeLa cell line using CRISPR-Cas9 and single-cell sorting. Our results confirmed the effective functioning of Rubik with Cre and Flp recombinases and showed the ability to induce selective recombination via either tamoxifen or trimethoprim. This system holds potential for neuroscience research, particularly in generating knock-in mice expressing Rubik, which could be instrumental in precisely defining the roles of specific neuronal populations based on Cre and Flp activity.
Two-dimensional perisaccadic visual mislocalization in rhesus macaque monkeys
Authors: Baumann Matthias P. , Hafed Ziad M. 
Published: November 2024

AbstractPerceptual localization of brief, high contrast perisaccadic visual probes is grossly erroneous. While this phenomenon has been extensively studied in humans, more needs to be learned about its underlying neural mechanisms. This ideally requires running similar behavioral paradigms in animals. However, during neurophysiology, neurons encountered in the relevant sensory and sensory-motor brain areas for visual mislocalization can have arbitrary, non-cardinal response field locations. This necessitates using mislocalization paradigms that can work with any saccade direction. Here, we first established such a paradigm in three male rhesus macaque monkeys. In every trial, the monkeys generated a visually-guided saccade towards an eccentric target. Once a saccade onset was detected, we presented a brief flash at one of three possible locations ahead of the saccade target location. After an experimentally-imposed delay period, we removed the saccade target, and the monkeys were then required to generate a memory-guided saccade towards the remembered flash location. All three monkeys readily learned the task, and, like humans, they all showed strong backward mislocalization towards the saccade target, which recovered for later flashes from the saccade time. Importantly, we then replicated a well-known property of human perisaccadic mislocalization, as revealed by two-dimensional mislocalization paradigms: that mislocalization is strongest for upward saccades. For horizontal saccades, we additionally found stronger mislocalization for upper visual field flashes, again consistent with humans. Our results establish a robust two-dimensional mislocalization paradigm in monkeys, and they pave the way for exploring the neural mechanisms underlying the dependence of perisaccadic mislocalization strength on saccade direction.SignificanceVisual perception is strongly altered around the time of saccades. Such alteration is often studied by characterizing how brief perisaccadic visual flashes are perceptually localized. While the properties of visual mislocalization have been exhaustively studied, the underlying neural mechanisms remain elusive, and this is due to a lack of suitable behavioral paradigms in animal models. We describe such a paradigm for macaques, which are ideal for exploring sensory-motor neural processes related to perisaccadic mislocalization.
Transcriptional Regulatory Logic Orchestrating Lymphoid and Myeloid Cell Fate Decisions
Authors: Godini Rasoul , Chopin Michaël 
Published: November 2024

AbstractThe differentiation of hematopoietic stem cells (HSCs) into diverse blood and immune cells is a complex, highly hierarchical process characterized by a series of tightly regulated steps. It involves a sequence of intermediate oligo-potent progenitors making successive binary decisions. This process gradually narrows down lineage possibilities until a final fate is reached. This step-wise process is tightly controlled by Transcription Factors (TFs) and their associated regulome ultimately resulting the differentiation of both lymphoid and myeloid compartments. Here, we set to unravel the lineage-specific gene regulatory circuitry controlling the development of B cells, T cells, Innate Lymphoid Cells (ILCs), and Dendritic Cells (DCs). We employ Weighted Gene Co-expression Network Analysis (WGCNA) to characterize gene modules associated to the lymphoid or myeloid cell fate, enabling the identification of lineage restricted TFs based on their expression patterns. By identifying TFs whose expression is subset-restricted or those with a broader expression in the hematopoietic compartment we construct a regulatory logic that potentially controls the development of these key immune cells. Our results point to conserved regulatory elements between ILCs, Natural Killer cells, and DCs. This analysis unravels an intricate relationship between each cell types and how the expression of key TFs dictate lineage specificity. We particularly dissect the elements associated to conventional DCs and plasmacytoid DCs. In conclusion, our findings shed new lights on regulatory mechanisms controlling blood cell development and offer a blueprint that can be leveraged to better understand the molecular mechanisms underpinning blood cell development.
Shared Rhythm to Shared Vision: Synchronous Marching increases Conformity on Perceptual Decision making
Authors: Biswas Manisha , Brass Marcel 
Published: November 2024

AbstractPrevious research on synchronous movement rituals have found that it increased prosocial propensity toward the synchronised group and reliance on the group opinions. However, whether basic cognitive processes such as perceptual decision-making are affected by synchronous movement remains unexplored. In this novel virtual reality experiment, we examined whether marching synchronously with a group can induce greater informational conformity on an unrelated perceptual task (forced choice random dot motion). We found higher degree of conformity following synchronised marching during low stimulus information. This finding indicates that synchronised movement induces minimal group membership expressed via increased self-other blurring and conformity. Thus, participation in movement-based rituals has the capacity to change our perception of the world to align more closely with the synchronised group.
The balance stabilising benefit of social touch: influence of an individual’s age and the partner’s relative body characteristics
Authors: Schulleri Katrin H. , Lee Dongheui , Johannsen Leif 
Published: November 2024

AbstractInterpersonal touch (IPT) is a successful strategy to support the stability of and individual’s body balance during a multitude of activities in daily life, including physical education and therapy. Despite common practice, however, the influence of an individual’s anthropometry and other personal characteristics, such as age, balancing skills, motor experience, and sex as well as interindividual differences in these characteristics between interaction partners on the balance stabilising benefit of social touch is unknown.We assessed an individual’s balance stability and change due to IPT provision during single-legged stance in 72 pairs (age range 4 to 63 years) under four sensory conditions: with or without vision in combination with IPT or without. Two participant subgroups were created: one of more vulnerable with low stability and one of more stable, mature participants. Best fitting multiple linear regression models, including moderating variables, for explaining the benefit of IPT in each visual condition indicated that without vision, an individual’s benefit of IPT was determined by their balancing skill and the partner-related difference in balancing skill but not by any other factors or partner-related differences. Especially vulnerable individuals improved considerably with IPT when vision was unavailable. With vision complementing IPT, however, an individual’s age-dependent motor developmental potential became an additional moderating factor.These findings indicate that the extent to which IPT is benefitting mutual balance stabilisation does not depend on biomechanical factors. Instead, the IPT benefit emerges as a product of both partners’ sensorimotor capabilities moderated by a person’s motor developmental potential when visual feedback could be utilised. We discuss a theoretical framework that accounts for the observed dependencies of the effect of haptic social support on balance control.Significance StatementThis work contributes to a better understanding of the factors influencing the benefit of interpersonal touch for balance stabilization across a wide age range from children to senior adults. It is the first work that investigates the role of both individual factors and relative differences between interaction partners on the amount of benefit of IPT and takes moderating factors such as age-related motor developmental potential, sex, and body mass index into account. Future studies can build on this work when selecting or simulating an optimal partner, especially for individuals with postural instability, for example due to age or sensorimotor deficits.
Anomalies in Hadronic
B
Decays
Authors: Berthiaume Raphaël , Bhattacharya Bhubanjyoti , Boumris Rida , Jean Alexandre , Kumbhakar Suman , London David 
Published: November 2024

In this Letter, we perform fits to B→PP decays, where B={B0,B+,Bs0} and the pseudoscalar P={π,K}, under the assumption of flavor SU(3) symmetry [SU(3)F]. Although the fits to ΔS=0 or ΔS=1 decays individually are good, the combined fit is very poor: there is a 3.6σ disagreement with the SU(3)F limit of the standard model (SMSU(3)F). One can remove this discrepancy by adding SU(3)F-breaking effects, but 1000% SU(3)F breaking is required. The above results are rigorous, group theoretically—no dynamical assumptions have been made. When one adds an assumption motivated by QCD factorization, the discrepancy with the SMSU(3)F grows to 4.4σ.

Published by the American Physical Society
2024
Leadership, values and authority: what shapes a manager’s work ethos and plays a key role in authority creation?
Authors: Postuła Agnieszka 
Published: November 2024

PurposeThe article aims to identify and classify competencies and values considered essential or even necessary in the daily work of active managers and leaders.Design/methodology/approachThis article is based on the results of comprehensive ethnographic research. I acquired the research material through open interviews (26 items), which facilitated immersion into the social reality of individual groups of respondents.FindingsA manager who consistently adheres to their values builds lasting authority. A manager who is guided by values also has a greater influence on decision-making and team relationships. A manager who adheres to values can contribute to improving organizational culture and thus increase employee engagement and achieve better organizational results.Research limitations/implicationsFindings from qualitative studies are context-specific and may not be easily generalizable to other settings or populations.Practical implicationsOrganizations need to recognize the centrality of values in building managerial authority. By strategically aligning values, promoting consistency and fostering a culture of trust, organizations can enhance managerial effectiveness, team relationships and overall organizational performance.Social implicationsManagers’ values play a crucial role in shaping workplace dynamics and can extend their influence to broader societal contexts. A values-driven approach has the potential to create positive social impacts and thus contribute to ethical business practices, trustful relationships and overall societal well-being.Originality/valueThe findings are original and valuable because they holistically explore the connection between managerial values and authority and identify key factors that contribute to lasting and impactful leadership in organizations and society.
Why the great surges of developments are different: a coupling analysis of the socio-political paradigm and the techno-economic paradigm
Authors: Yang Hutao , Yang Mingxuan 
Published: November 2024

PurposeThe “problem-ridden present” is indubitably related to the prevalence of neoliberalism, but it is also when the fifth technological revolution took place. In this special time, what should be done to achieve the coupling of Socio-political Paradigmand Technoeconomic Paradigm through the adaptive transformation of socio-political paradigm to avoid the resurfacing of the “problem-ridden present”?Design/methodology/approachThe obvious difference between the efficacy of the fifth technological revolution and the previous four indicates that the Techno-economic Paradigm of Neo-Schumpeterian school is not sufficient enough to explain the institutional obstacles. No matter institutional or technological, any single perspective would not be adequate to explain the fundamental cause of the “problem-ridden present”.FindingsFrom a historical materialism standpoint, the structure and progress of the techno-economic paradigm are inevitably influenced by the socio-political paradigm, and only when the techno-economic paradigm and the socio-political paradigm are coupled, the potential of the wave of technological revolution can be “shared release.”Originality/valueThe coupling analysis of the Socio-political Paradigm and the Technoeconomic Paradigm is of great enlightening value to the understanding of the economic development in the Digital Economy era.
The financialization of platform capital from the perspective of political economy
Authors: Qi Hao , Li Zhongjin , Wen Yankai 
Published: November 2024

PurposeThe financialization of the platform economy is a crucial part of the theoretical landscape of the platform economy. However, it has not yet received adequate attention from the academic community. This deficiency has led to current research often neglecting the supportive role of finance capital in the platform economy, and consequently, there has been a lack of comprehensive interpretation of the generally poor profitability of platform companies. This paper aims to address the existing gap in the literature within this field.Design/methodology/approachThis paper examines the role of finance capital, particularly venture capital, in the platform economy and offers a political economy analysis of the historical background, accumulation logic, and inherent contradictions of the financialization of the platform economy.FindingsThe paper argues that the advancement of new technologies, excess capital and labor supply in the post-crisis era, and the development of economic financialization, particularly the maturity and diffusion of the venture capital system, constitute the historical background of the financialization of the platform economy. Driven by finance capital, platform enterprises engage in excessive expansion to increase their financial valuations, forming a valuation-driven accumulation model. Financialization has caused the platform economy to deviate from its normal developmental trajectory. Platform enterprises exhibit higher shareholder payouts while simultaneously engaging in rapid investment expansion, leading to a trend of bubble-like development in the platform economy.Originality/valueThe financialization of the platform economy has exacerbated the socio-economic problems caused by disembeddedness, deepening the contradiction between the speculative logic of finance capital and the healthy development of the real economy. This profoundly reflects the obstruction posed by contemporary capitalist relations of production to the development of productive forces. This paper provides several key policy insights for the formulation and implementation of relevant policies in China.
Managing coworker conflict: investigating the effect of workplace phubbing and mindfulness on employee deviant and negligent behavior
Authors: Alagarsamy Subburaj , Mehrolia Sangeeta , Vinod Sangeetha 
Published: November 2024

PurposeThis study aims to examine the influence of workplace phubbing on employee deviant behavior and negligence, while also investigating the mediating role of coworker conflict. Additionally, the study explores the moderating effect of workplace mindfulness on the relationship between workplace phubbing, the mediators and employee deviant behavior and negligence.Design/methodology/approachData were gathered from employees in the service sector in the UAE using an online survey questionnaire. A total of 374 participants submitted complete responses. The study’s hypotheses were tested through regression-based moderated path analysis, incorporating conditional process modeling and nonlinear bootstrapping.FindingsThe study indicates that experiencing “phubbing” at work contributes to feelings of coworker conflict, which subsequently leads to increased interpersonal deviance and employee negligence. Moreover, workplace mindfulness weakens the positive influence of being phubbed on coworker conflict, interpersonal deviance and employee negligence.Originality/valueTo the best of the authors’ knowledge, no previous studies have examined the negative impact of being “phubbed” at the individual employee level within the service industry. This study aims to contribute to both theory and practice by elucidating the mediating mechanism of coworker conflict and exploring the moderating effects of workplace mindfulness.
The role of interpersonal distrust as a mediator of the relationship between ethnic minorities and knowledge hiding in international construction projects
Authors: Anbar Diana R. , Chang Tengyuan , Ullah Safi , Alqershy Mohammed Taha , Mousa Kawther , Deng Xiaopeng 
Published: November 2024

PurposeThis study explores the factors influencing knowledge hiding (KH) in international construction projects (ICPs), focussing on the role of ethnic minorities (EMs) and interpersonal distrust (ID). It also aims to understand how these factors influence KH and explore the mediating effect of ID on the relationship between EMs and KH.Design/methodology/approachA survey questionnaire was developed and distributed to professionals participating in ICPs. We used structural equation modelling (SEM) to analyse 294 valid responses, examining the effect of EMs, ID and demographic variables on KH behaviours. Our approach integrates insights from social identity theory (SIT) and actor network theory (ANT) to provide a comprehensive framework for understanding the observed dynamics.FindingsThe findings reveal that EMs significantly influence KH behaviours, with ID partially mediating this relationship. Additionally, demographic variables such as gender and age also play significant roles in KH behaviours. However, the degree of education did not show a significant impact on KH. Our results emphasise the critical importance of addressing ID and fostering inclusive environments to reduce KH.Research limitations/implicationsWhile our study sheds light on the complex dynamics of KH in ICPs, further research could explore additional contextual factors and organisational influences. While our study emphasises the importance of inclusive practices and trust-building to mitigate KH, it also recognises the need for a deeper understanding of power dynamics and organisational contexts.Practical implicationsOur findings underscore the importance of creating transparent and inclusive cultures of knowledge sharing (KS). Implementing inclusion initiatives and providing cultural awareness training can enhance project outcomes and organisational success. We also propose a practical framework to mitigate KH by promoting trust, inclusivity and effective KS practices.Originality/valueThis study contributes to the understanding of knowledge management in multicultural project teams by integrating SIT and ANT, offering a comprehensive perspective on the factors driving KH in ICPs. The findings pave the way for future investigations into power dynamics and organisational contexts within ICPs.