bluuebunny/crossref_metadata_2025_split huggingface dataset:
Trapping intermediates in metal transfer reactions of the CusCBAF export pump of Escherichia coli
Authors: Chacón Kelly N. , Perkins Jonathan , Mathe Zachary , Alwan Katherine , Ho Ethan N. , Ucisik Melek N. , Merz Kenneth M. , Blackburn Ninian J. 
Published: November 2018

AbstractEscherichia coli CusCBAF represents an important class of bacterial efflux pump exhibiting selectivity towards Cu(I) and Ag(I). The complex is comprised of three proteins: the CusA transmembrane pump, the CusB soluble adaptor protein, and the CusC outer-membrane pore, and additionally requires the periplasmic metallochaperone CusF. Here we used spectroscopic and kinetic tools to probe the mechanism of copper transfer between CusF and CusB using selenomethionine labeling of the metal-binding Met residues coupled to RFQ-XAS at the Se and Cu edges. The results indicate fast formation of a protein−protein complex followed by slower intra-complex metal transfer. An intermediate coordinated by ligands from each protein forms in 100 ms. Stopped-flow fluorescence of the capping CusF-W44 tryptophan that is quenched by metal transfer also supports this mechanism. The rate constants validate a process in which shared-ligand complex formation assists protein association, providing a driving force that raises the rate into the diffusion-limited regime.
IFNL4-ΔG is associated with prostate cancer among men at increased risk of sexually transmitted infections
Authors: Minas Tsion Zewdu , Tang Wei , Smith Cheryl J. , Onabajo Olusegun O. , Obajemu Adeola , Dorsey Tiffany H. , Jordan Symone V. , Obadi Obadi M. , Ryan Bríd M. , Prokunina-Olsson Ludmila , Loffredo Christopher A. , Ambs Stefan 
Published: November 2018

AbstractSexually transmitted infections can reach the prostate gland where their harmful effects are mediated by innate immunity, including interferons. Humans are polymorphic for the germline dinucleotide variant, rs368234815-TT/ΔG, in the IFNL4 gene encoding interferon λ4. Since the IFNL4-ΔG allele has been linked to impaired viral clearance, we hypothesized that potential exposure to sexually transmitted pathogens, as assessed by the number of lifetime sexual partners, may increase prostate cancer risk in an IFNL4-ΔG-dependent manner. Accordingly, we find that men with 10 or more sexual partners and at least one copy of IFNL4-ΔG have a significantly increased risk of prostate cancer while those with the same number of partners but lacking IFNL4-ΔG do not. Moreover, a test for effect modification shows a positive interaction between the number of lifetime partners and IFNL4-ΔG in the development of aggressive prostate cancer. Based on these findings, we conclude that a gene–environment interaction between IFNL4-ΔG and sexual activity may increase the risk of prostate cancer.
A Survey of Clinician Decision Making When Identifying Swallowing Impairments and Determining Treatment
Authors: Vose Alicia K. , Kesneck Sara , Sunday Kirstyn , Plowman Emily , Humbert Ianessa 
Published: November 2018

Purpose
Speech-language pathologists (SLPs) are the primary providers of dysphagia management; however, this role has been criticized with assertions that SLPs are inadequately trained in swallowing physiology (Campbell-Taylor, 2008). To date, diagnostic acuity and treatment planning for swallowing impairments by practicing SLPs have not been examined. We conducted a survey to examine how clinician demographics and swallowing complexity influence decision making for swallowing impairments in videofluoroscopic images. Our goal was to determine whether SLPs' judgments of swallowing timing impairments align with impairment thresholds available in the research literature and whether or not there is agreement among SLPs regarding therapeutic recommendations.

Method
The survey included 3 videofluoroscopic swallows ranging in complexity (easy, moderate, and complex). Three hundred three practicing SLPs in dysphagia management participated in the survey in a web-based format (Qualtrics, 2005) with frame-by-frame viewing capabilities. SLPs' judgments of impairment were compared against impairment thresholds for swallowing timing measures based on 95% confidence intervals from healthy swallows reported in the literature.

Results
The primary impairment in swallowing physiology was identified 67% of the time for the easy swallow, 6% for the moderate swallow, and 6% for the complex swallow. On average, practicing clinicians mislabeled 8 or more swallowing events as impaired that were within the normal physiologic range compared with healthy normative data available in the literature. Agreement was higher among clinicians who report using frame-by-frame analysis 80% of the time. A range of 19–21 different treatments was recommended for each video, regardless of complexity.

Conclusions
Poor to modest agreement in swallowing impairment identification, frequent false positives, and wide variability in treatment planning recommendations suggest that additional research and training in healthy and disordered swallowing are needed to increase accurate dysphagia diagnosis and treatment among clinicians.
Strategien zur Überwindung der erworbenen EGFR-TKI-Resistenz durch T790M spezifische Substanzen am Beispiel von Osimertinib
Authors: Griesinger F. , Radke S. , Lüers A. , Deschler-Baier B. , Kimmich M. , Sebastian M. , Schulz C. , Brugger W. , Wiewrodt R. , Pirker R. , Früh M. , Gautschi O. , Wolf J. 
Published: November 2018

Zusammenfassung Die Resistenzbildung gegenüber der 1. und 2. Generation von Tyrosinkinase-Inhibitoren (TKIs) des epidermalen Wachstumsfaktors (EGFR) stellt bei der Behandlung von Patienten mit nicht kleinzelligem Lungenkarzinom, die eine aktivierende Mutation im EGFR aufweisen, ein großes Problem dar. Drittgenerations-EGFR-TKIs richten sich sowohl gegen aktivierende als auch gegen die Resistenz-vermittelnde T790M-Mutation. EGFR-TKIs der 3. Generation zeigen in klinischen Studien bei T790M-positiven Patienten relevante Wirksamkeit bei meist milden bis moderaten klassenspezifischen Nebenwirkungen. Molekularpathologischen Analysen kommt bei der Entscheidung zur Therapie mit Drittgenerations-EGFR-TKIs eine bedeutsame Rolle zu. In dieser Übersichtsarbeit wird der aktuelle Entwicklungsstand von Drittgenerations-EGFR-TKIs dargestellt mit einem Schwerpunkt auf Osimertinib, dem ersten und bislang einzigen in Deutschland zugelassenen Wirkstoff dieser Klasse. Zudem wird die Relevanz einer molekularen Diagnostik an Tumorgewebe bzw. an zirkulierender Tumor-DNA diskutiert.
Stimm- und Kehlkopfveränderungen im Alter (Presbyphonie und
Presbylarynx)
Authors: Angerstein Wolfgang 
Published: November 2018

ZusammenfassungEtwa 20 % der über 60-Jährigen weisen eine Altersstimmstörung (Presbyphonie) auf,
die oftmals von Presbyakusis (Altersschwerhörigkeit), Presbyphagie
(Schluckstörungen im Alter), Presbyvertigo (Altersschwindel) und/oder Presbyopie
(Alterssichtigkeit) begleitet wird.Presbyphonie betrifft die Singstimme häufiger, früher und deutlicher als die
Sprechstimme. Aber nicht nur Qualität und Belastbarkeit von Sing- und
Sprechstimme werden beeinträchtigt, auch der Atemantrieb wird durch die
eingeschränkte Lungenfunktion schwächer. Durch das Absenken des Kehlkopfes
verändern sich bei Senioren zudem die Resonanzverhältnisse im Ansatzrohr, und
das Aspirationsrisiko steigt.Die stimmlichen Einbußen beruhen auf morphologischen Altersveränderungen des
Kehlkopfes (Prebylarynx), die sowohl das ektolaryngeale Kehlkopfskelett als auch
die endolaryngealen Weichteile (insbesondere die Stimmlippen) betreffen.Der Vokalismuskel atrophiert und wird hypoton, was zu einer
spindelförmig-ovalären Glottisschlussinsuffizienz mit Luftverlust bei Phonation
führt. Die Schleimhäute trocknen aus im Sinne einer Laryngitis sicca.Bei der Stimmrehabilitation im Alter müssen allgemeine und speziell auf die
Stimme bezogene Behandlungsoptionen unterschieden werden: Zu den allgemeinen
Therapieansätzen gehört die Mitbehandlung internistischer und neurologischer
Grunderkrankungen (z. B. Reflux, neurodegenerative oder zerebrovaskuläre
Erkrankungen) – auch Nebenwirkungen von Medikamenten müssen beachtet werden. Ein
gesunder Lebensstil ohne Noxen begünstigt den Funktionserhalt von Phonations-
und Atemorganen. Stimmtherapeutisch kommen sowohl Tonisierungsübungen zur
Verbesserung des phonatorischen Glottisschlusses als auch detonisierende
Entspannungsübungen zum Abbau supraglottischer hyperfunktioneller
Pressmechanismen in Betracht. In Einzelfällen mit großem Glottisspalt bei
Phonation kann eine phonochirurgische Stimmlippenaugmentation den Glottisschluss
verbessern und somit die Stimme kräftigen. Stimmhygienische Maßnahmen (z. B.
regelmäßiges Befeuchten der Atemwege) ergänzen das Stimmtraining.Singen im Alter hat sowohl psychosozial (Steigerung des Wohlbefindens und der
sozialen Teilhabe) als auch immunologisch (Anstieg des IgA im Speichel) positive
Effekte.
Die Deutsche Dermatologische Akademie und ihre Zertifikate
Authors: Fritz K. 
Published: November 2018

ZusammenfassungNeben Facharzt- und Zusatzbezeichnungen der Ärztekammern gibt es eine Fülle von Spezialkenntnissen, die mit den offiziellen Titeln nicht abgebildet werden. Qualifikationsbelege in Form von Zertifikaten können als Kompetenznachweis gegenüber hilfesuchenden Patienten sowie in den Medien Bedeutung haben, wie auch in juristischen Fragen wie Haftung, für die Kostenerstattung und zum Marketing genutzt werden.Hierzu wurden in der DDA (Deutsche Dermatologische Akademie) eine Reihe von Themen identifiziert, die für solche Zusatzweiterbildungen und Zertifizierungen von Interesse sind, für die nun Curricula existieren und Fortbildungsveranstaltungen danach ausgerichtet werden können. Nach ca. 10 Jahren sollte eine Rezertifizierung erfolgen. Auch unser Assistenzpersonal soll von diesen Weiterbildungen profitieren, daher werden spezielle Curricula und Zertifikate auch für sie aufgelegt.
Oropharynx-Karzinom-CT: prognostische Bedeutung der
extranodalen Ausbreitung
Authors: Güldner Christian 
Published: November 2018

Patel MR et al. Radiographic Imaging Does Not Reliably Predict Macroscopic
Extranodal Extension in Human Papilloma Virus-Associated Oropharyngeal Cancer.
ORL 2018; 80: 85–95
Amerikanische Radiologen und Fachärzte für Otorhinolaryngologie evaluierten
bei Patienten mit Oropharynx-Plattenepithelkarzinomen (OPSCC) die
Genauigkeit der präoperativen kontrastmittelverstärkten Computertomographie
(CECT), um die extranodale Ausdehnung (ENE) (> 2 mm) zu
prognostizieren.
Sexueller Missbrauch an Kindern – Entwicklungen im Hell- und Dunkelfeld seit 1953 und Implikationen für das Gesundheitswesen
Authors: Horten Barbara , Dölling Dieter , Hermann Dieter , Kruse Andreas , Schmitt Eric , Bannenberg Britta , Salize Hans-Joachim , Dreßing Harald 
Published: November 2018

ZusammenfassungDaten zum sexuellen Missbrauch an Kindern können zum einen über die Hellfeldstatistiken gewonnen und zum anderen durch Dunkelfeldforschung erhoben werden. Der vorliegende Beitrag befasst sich mit der längsschnittlichen Entwicklung der Delikte in den vergangenen 63 Jahren in Deutschland. Die Straftaten des sexuellen Missbrauchs von Kindern gemäß § 176 StGB weisen in der Polizeilichen Kriminalstatistik (PKS) seit Veröffentlichung der PKS 1953–2016 einen Rückgang der Häufigkeitszahlen auf. Die Ergebnisse aus der Dunkelfeldforschung sind uneinheitlich, sprechen aber teilweise auch für einen Rückgang. Die Bedeutung dieser Befunde für die Präventionsarbeit und gesundheitspolitische Aktivitäten wird diskutiert.
Organocatalytic Group Transfer Reactions with Hypervalent Iodine­ Reagents
Authors: Kalek Marcin , Ghosh Manoj , Rajkiewicz Adam 
Published: November 2018

In recent years, a plethora of synthetic methods that employ hypervalent iodine compounds donating an atom or a group of atoms to an acceptor molecule have been developed. Several of these transformations utilize organocatalysis, which complements well the economic and environmental advantages offered by iodine reagents. This short review provides a systematic survey of the organocatalytic approaches that have been used to promote group transfer from hypervalent iodine species. It covers both the reactions in which an organocatalyst is applied to activate the acceptor, as well as those that exploit the organocatalytic activation of the hypervalent iodine reagent itself.1	Introduction2	Organocatalytic Activation of Acceptor2.1	Amine Catalysis via Enamine and Unsaturated Iminium Formation2.2	NHC Catalysis via Acyl Anion Equivalent and Enolate Formation2.3	Chiral Cation Directed Catalysis and Brønsted Base Catalysis via Pairing with Stabilized Enolates3	Organocatalytic Activation of Hypervalent Iodine Reagent3.1	Brønsted and Lewis Acid Catalysis3.2	Lewis Base Catalysis3.3	Radical Reactions with Organic Promoters and Catalysts4	Summary and Outlook
Liposarcoma paratesticular gigante
Authors: Cortes-Caballero Carlos , Mogollon-Zehr Margarita Rosa , Campos Silvia Nathalia Vera 
Published: November 2018

Introducción Los liposarcomas son lesiones que se originan en el tejido mesodérmico, su localización paratesticular es muy rara. Se divide en 4 subtipos histológicos, siendo el mixoide el más infrecuente. Tiene una recurrencia del 70%, Por lo que su tratamiento inicial debe ser agresivo.
Objetivo resaltar su gran tamaño y que sea considerado como diagnóstico diferencial de masa inguinoescrotal.
Presentación del caso Paciente masculino de 87 años con masa dolorosa inguinoescrotal derecha con diagnóstico de hernia inguinal unilateral, se realiza orquiectomía inguinal derecha. En patología se recibe pieza quirúrgica de 2440 g; al estudio histológico se observa lesión tumoral mesenquimal maligna, rodeado por estroma nodular mixoide prominente. Se diagnostica liposarcoma mixoide de cordón espermático. (Figura 2).
Conclusiones Son neoplasias raras mal diagnosticadas como hernia inguinal, escrotal o lipoma de la médula espermática. Tiene un alto riesgo de metástasis, siendo la orquiectomía inguinal con resección del cordón espermático el manejo quirúrgico ideal.
A small single-domain protein folds through the same pathway on and off the ribosome
Authors: Guinn Emily J. , Tian Pengfei , Shin Mia , Best Robert B. , Marqusee Susan 
Published: November 2018

Significance
All proteins are synthesized by the ribosome and therefore have the opportunity to fold for the first time while they are still attached to it. Although protein folding in vitro has been extensively characterized, we are just beginning to understand how proteins fold on the ribosome, which is essential to understanding protein folding in vivo. Here, we characterize and compare the folding pathway of a single protein domain on the ribosome and in bulk solution. By probing the response of the protein to mechanical force, chemical denaturant, and point mutations, and comparing experimental results to simulations, we show that this single protein domain folds through the same pathway on and off the ribosome.
Thermal and durability performances of mortar and concrete containing phase change materials
Authors: Drissi Sarra , Ling Tung-Chai 
Published: November 2018

Abstract
Thanks to their latent heat storage, phase change materials (PCMs) continue to attract the interest of many researchers and industrialists. Microencapsulated and macroencapsulated PCMs are generally added directly or immersed into conventional buildings materials to improve thermal inertia and provide a better comfort to users. However, many drawbacks related to PCMs were noticed when they are incorporated into cement-based materials. This paper summarizes previous research concerning the use of PCMs on the thermal, mechanical and durability properties of mortar and concrete. Despite the obvious reduction of the mechanical strength, PCMs are still found to be good candidates to enhance the overall thermal performances of cement-based materials. It is therefore suggested that future researches should focus more on mitigating the impact of PCMs on the mechanical strength in order to gain a wider application of PCMs in concrete building applications.
Effect of binder content and water-binder ratio in mortar developed using partial replacement of cement with palm oil clinker powder
Authors: Sumesh Mathialagan , Alengaram U Johnson , Nayaka Ramappa Ramesh 
Published: November 2018

Abstract
The manufacture of cement leads to almost 5 to 7% of the total production of carbon dioxide which causes greenhouse effect. So, it is vital to replace the ordinary Portland cement by the other binders which have the similar or better properties than cement. Besides, the recent interests on the palm oil industrial wastes in the development of mortar led to research works on usefulness of these wastes in construction materials. The palm oil industry produces many wastes such as oil palm shell, palm oil fuel ash (POFA) and palm oil clinker powder (POCP). In this research work, an experimental study was conducted to investigate the effects of different binder contents, and water-binder (w/b) ratio on the development of mortars. Conventional ordinary Portland cement was replaced with 40% of POCP and the total binder contents of 450, 500 and 550 kg/m3 with w/b ratios of 0.45, 0.35 and 0.32 used. The properties of mortars were evaluated for fresh and hardened properties; XRD characteristic was also studied. Test results showed that the strength of mortar specimens improved as the binder content increased with subsequent decrease in w/b ratio. POCP mortar specimens with binder content of 550kg/m3 with cement replacement of 40% produced 28-day compressive strength of 45.3 MPa, compared to 65.12 MPa for control cement mortar (CM). The value of flow diameter of 40% replacement of POCP mortar was 108.51 mm and this was 16.6% lower compared to control mortar. Though POCP based mortar produced a strength reduction of about 36.6%, its usefulness in achieving acceptable strength and sustainable aspect of reducing 40% of conventional cement could be considered significant.
Pan-genomic analysis provides novel insights into the association of E.coli with human host and its minimal genome
Authors: Yang Zhi-Kai , Luo Hao , Zhang Yanming , Wang Baijing , Gao Feng 
Published: November 2018

Abstract

Motivation
Bacteria can usually acquire certain advantageous genes that enable the bacteria to adapt to rapidly changing niches, thereby leading to a wide range of intraspecific genome content and genetic redundancy. The minimal genome of Escherichia coli, which is the most important bacterial species, and the association between E.coli and its human host are worthy of further exploration.

Results
We used gene prediction and phylogenetic analysis to reveal a rich phylogenetic diversity among 491 E.coli strains and to reveal substantial differences between these strains with respect to gene number and genome length. We used pan-genomic analysis to accurately identify 867 core genes, in which only 243 genes are shared by essential genes. This analysis revealed that core genes mainly provide essential functions to the basic lifestyle of E.coli, and accessory genes are likely to confer selective advantages such as niche adaptation or the ability to colonize specific hosts. By association analysis, we found that E.coli strains in non-human hosts may more easily utilize foreign genetic materials to adapt to their surroundings, but the population in human hosts has higher demands for the control of population density, indicating that highly accurate quorum-sensing behavior is very important for harmony between E.coli and its human host. By considering core genes and previous deletions together, we proposed a potential direction for further reduction of the E.coli genome.

Availability and implementation
The data, analysis process and detailed information on software tools used in this study are all available in the supplementary material.

Supplementary information
Supplementary data are available at Bioinformatics online.
ERA-EDTA fellowship, a ‘bonne opportunité’: the scientific and human experience of a fellow
Authors: Trepiccione Francesco 
Published: November 2018

Abstract
As a fellow of the ERA-EDTA long-term fellowship programme, I spent two fantastic years as a post-doc in Prof. D. Eladari’s laboratory at Inserm U970, Paris-Cardiovascular Research Centre. It was a highly formative and productive scientific experience. On a personal level, immersion into the French society and the international environment of the laboratory were added bonuses that enriched my experience. I am honoured to report my experience here from the ERA-EDTA fellowship programme. I hope this will inspire young fellows to apply for such a programme and pursue their career in science. Good mentorship, a passion for scientific investigation and determination are required.
Non-linear multidimensional flow cytometry analyses delineate NK cell phenotypes in normal and HIV-infected chimpanzees
Authors: Manickam Cordelia , Li Haiying , Shah Spandan V , Kroll Kyle , Reeves R Keith 
Published: November 2018

Abstract
Natural killer (NK) cells are primary immune effector cells with both innate and potentially adaptive functions against viral infections, but commonly become exhausted or dysfunctional during chronic diseases such as human immunodeficiency virus (HIV). Chimpanzees are the closest genetic relatives of humans and have been previously used in immunology, behavior and disease models. Due to their similarities to humans, a better understanding of chimpanzee immunology, particularly innate immune cells, can lend insight into the evolution of human immunology, as well as response to disease. However, the phenotype of NK cells has been poorly defined. In order to define NK cell phenotypes, we unbiasedly quantified NK cell markers among mononuclear cells in both naive and HIV-infected chimpanzees by flow cytometry. We identified NKG2D and NKp46 as the most dominant stable NK cells markers using multidimensional data reduction analyses. Other traditional NK cell markers such as CD8α, CD16 and perforin fluctuated during infection, while some such as CD56, NKG2A and NKp30 were generally unaltered by HIV infection, but did not delineate the full NK cell repertoire. Taken together, these data indicate that phenotypic dysregulation may not be pronounced during HIV infection of chimpanzees, but traditional NK cell phenotyping used for both humans and other non-human primate species may need to be revised to accurately identify chimpanzee NK cells.
Single-cell analyses identify distinct and intermediate states of zebrafish pancreatic islet development
Authors: Lu Chong-Jian , Fan Xiao-Ying , Guo Yue-Feng , Cheng Zhen-Chao , Dong Ji , Chen Jin-Zi , Li Lian-Yan , Wang Mei-Wen , Wu Ze-Kai , Wang Fei , Tong Xiang-Jun , Luo Ling-Fei , Tang Fu-Chou , Zhu Zuo-Yan , Zhang Bo 
Published: November 2018

AbstractPancreatic endocrine islets are vital for glucose homeostasis. However, the islet developmental trajectory and its regulatory network are not well understood. To define the features of these specification and differentiation processes, we isolated individual islet cells from TgBAC(neurod1:EGFP) transgenic zebrafish and analyzed islet developmental dynamics across four different embryonic stages using a single-cell RNA-seq strategy. We identified proliferative endocrine progenitors, which could be further categorized by different cell cycle phases with the G1/S subpopulation displaying a distinct differentiation potential. We identified endocrine precursors, a heterogeneous intermediate-state population consisting of lineage-primed alpha, beta and delta cells that were characterized by the expression of lineage-specific transcription factors and relatively low expression of terminally differentiation markers. The terminally differentiated alpha, beta, and delta cells displayed stage-dependent differentiation states, which were related to their functional maturation. Our data unveiled distinct states, events and molecular features during the islet developmental transition, and provided resources to comprehensively understand the lineage hierarchy of islet development at the single-cell level.