bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biomedical Applications of Layer‐by‐Layer Self‐Assembly for Cell Encapsulation: Current Status and Future Perspectives
Authors: Liu Tengfei , Wang Ying , Zhong Wen , Li Bingyun , Mequanint Kibret , Luo Gaoxing , Xing Malcolm 
Published: December 2018

AbstractEncapsulating living cells within multilayer functional shells is a crucial extension of cellular functions and a further development of cell surface engineering. In the last decade, cell encapsulation has been widely utilized in many cutting‐edge biomedical fields. Compared with other techniques for cell encapsulation, layer‐by‐layer (LbL) self‐assembly technology, due to the versatility and tunability to fabricate diverse multilayer shells with controllable compositions and structures, is considered as a promising approach for cell encapsulation. This review summarizes the state‐of‐the‐art and potential future biomedical applications of LbL cell encapsulation. First of all, a brief introduction to the LbL self‐assembly technique, including assembly mechanisms and technologies, is made. Next, different cell encapsulation strategies by LbL self‐assembly techniques are explained. Then, the biomedical applications of LbL cell encapsulation in cell‐based biosensors, cell transplantation, cell/molecule delivery, and tissue engineering, are highlighted. Finally, discussions on the current limitations and future perspectives of LbL cell encapsulation are also provided.
Simulating Inflammation in a Wound Microenvironment Using a Dermal Wound‐on‐a‐Chip Model
Authors: Biglari Sahar , Le Thi Y. L. , Tan Richard P. , Wise Steven G. , Zambon Alessandro , Codolo Gaia , De Bernard Marina , Warkiani Majid , Schindeler Aaron , Naficy Sina , Valtchev Peter , Khademhosseini Ali , Dehghani Fariba 
Published: December 2018

AbstractConsiderable progress has been made in the field of microfluidics to develop complex systems for modeling human skin and dermal wound healing processes. While microfluidic models have attempted to integrate multiple cell types and/or 3D culture systems, to date they have lacked some elements needed to fully represent dermal wound healing. This paper describes a cost‐effective, multicellular microfluidic system that mimics the paracrine component of early inflammation close to normal wound healing. Collagen and Matrigel are tested as materials for coating and adhesion of dermal fibroblasts and human umbilical vein endothelial cells (HUVECs). The wound‐on‐chip model consists of three interconnecting channels and is able to simulate wound inflammation by adding tumor necrosis factor alpha (TNF‐α) or by triculturing with macrophages. Both the approaches significantly increase IL‐1β, IL‐6, IL‐8 in the supernatant (p < 0.05), and increases in cytokine levels are attenuated by cotreatment with an anti‐inflammatory agent, Dexamethasone. Incorporation of M1 and M2 macrophages cocultured with fibroblasts and HUVECs leads to a stimulation of cytokine production as well as vascular structure formation, particularly with M2 macrophages. In summary, this wound‐on‐chip system can be used to model the paracrine component of the early inflammatory phase of wound healing and has the potential for the screening of anti‐inflammatory compounds.
Engineering Transport in Manganites by Tuning Local Nonstoichiometry in Grain Boundaries
Authors: Chiabrera Francesco , Garbayo Iñigo , López‐Conesa Lluis , Martín Gemma , Ruiz‐Caridad Alicia , Walls Michael , Ruiz‐González Luisa , Kordatos Apostolos , Núñez Marc , Morata Alex , Estradé Sonia , Chroneos Alexander , Peiró Francesca , Tarancón Albert 
Published: December 2018

AbstractInterface‐dominated materials such as nanocrystalline thin films have emerged as an enthralling class of materials able to engineer functional properties of transition metal oxides widely used in energy and information technologies. In particular, it has been proven that strain‐induced defects in grain boundaries of manganites deeply impact their functional properties by boosting their oxygen mass transport while abating their electronic and magnetic order. In this work, the origin of these dramatic changes is correlated for the first time with strong modifications of the anionic and cationic composition in the vicinity of strained grain boundary regions. We are also able to alter the grain boundary composition by tuning the overall cationic content in the films, which represents a new and powerful tool, beyond the classical space charge layer effect, for engineering electronic and mass transport properties of metal oxide thin films useful for a collection of relevant solid‐state devices.
3D‐Printed Mechanical Metamaterials with High Energy Absorption
Authors: Yuan Shangqin , Chua Chee Kai , Zhou Kun 
Published: December 2018

AbstractRecently, 3D metamaterials have been achieved with inaccessible mechanical properties in natural materials such as negative Poisson's ratio, stiffness, and thermal expansion coefficient. While most of the developed metamaterials are with engineerable deformation evolution of structures, few studies have revealed their potential in energy absorption due to the limited mechanical properties of 3D‐printed constituent materials and inevitable structural defects induced by the manufacturing process. Herein, an approach is proposed for creating 3D metamaterials of auxetic composite lattices via laser‐sintering of carbon nanotubes reinforced nanocomposites, which provide a platform for the design and manufacturing of systems with programmable energy absorption capability. The optimization of constituent material and structural design enables the improvement of energy absorption performance across multiple scales. The energy absorption capacity of auxetic metamaterials was exponentially scaled with the relative density with the order of 2.5–3. The rationally topologized auxetic metamaterials exhibit a combination of high specific densification strength (0.0195 MPa kg−1 m−3), ultrahigh energy absorption capacity (6.29 MJ m−3), and excellent specific energy absorption (20.42 J g−1). Impressively, this group of auxetic metamaterials possesses the advantageous specific energy absorption approaching that of titanium alloy foams as well as over a broad range of materials including plastic foams, aluminum alloy foams, and other 3D‐printed lightweight structures.
A Wearable Second Skin‐Like Multifunctional Supercapacitor with Vertical Gold Nanowires and Electrochromic Polyaniline
Authors: An Tiance , Ling Yunzhi , Gong Shu , Zhu Bowen , Zhao Yunmeng , Dong Dashen , Yap Lim Wei , Wang Yan , Cheng Wenlong 
Published: December 2018

AbstractAn ideal wearable/implantable bio‐diagnostics can be powered by second skin‐like energy devices in that they offer advantages such as conformal attachment/integration, lightweightness, and moduli‐matching properties. Past several years have witnessed encouraging progresses in soft stretchable supercapacitors by various material combinations and design strategies; however, it remains nontrivial to achieve highly flexible high‐performance supercapacitors in a skin‐thin layout yet with multifunctionality. Here, such a second skin‐like electrochromic supercapacitor is demonstrated using self‐assembled vertical gold nanowires (v‐AuNWs) and electrodeposited polyaniline (PANI). The v‐AuNW film is highly conductive yet offers great flexibility and deformability, while PANI plays a dual role in enhancing capacitance and as charge/discharge‐level indicator. The assembled supercapacitors offer superior skin‐conformability and could achieve an areal specific capacitance of 11.76 mF cm−2 (scan rate of 10 mV s−1) with high durability. A second skin‐like wearable tattoo energy device can be designed with negligible performance deterioration under multiple hand movements and skin deformations.
Hybrid Additive Manufacturing Method for Selective Plating of Freeform Circuitry on 3D Printed Plastic Structure
Authors: Li Ji , Wang Yang , Xiang Gengzhao , Liu Handa , He Jiangling 
Published: December 2018

AbstractThis work proposes a hybrid additive manufacturing method coupling the advantages of dual‐material fused deposition modeling (FDM) 3D printing and selective electroless plating. The method relies on the selective sensitization/activation and metallization of dual‐material FDM 3D printed structure. High‐conductive nickel alloy can only be deposited on the platable plastic surface leaving the nonplatable plastic bare. The study reveals that a combination of acrylonitrile‐butadiene‐styrene and polyethylene terephthalate glycol‐modified materials provides the best metallization selectivity and film adhesion strength. The effects of electroless plating parameters such as temperature, pH value, and duration on the electrical conductivity of metallic film are systematically investigated. Through four‐point probe measurement, energy‐dispersive X‐ray spectroscopy inspection, and scanning electron microscopy cross‐section analysis, it is found that the metal deposition rate majorly depends on the plating temperature, while the composition of plated film is mainly determined by the pH value. Thus, the conductivity of metallic film can be customized according to the detailed requirements of practical applications. A minimal resistivity of 6.7 × 10−5 Ω cm is obtained when electrolessly plated at 50 °C and pH 9. A light‐emitting diode (LED) blinking circuitry is fabricated in both 2D and 3D manners as demonstrators to prove the feasibility and potential of this technology in industry applications.
Controlled Nonvolatile Transition in Polyoxometalates‐Graphene Oxide Hybrid Memristive Devices
Authors: Chen Xiaoli , Zhu Xin , Zhang Shi‐Rui , Pan Jingyi , Huang Pu , Zhang Chen , Ding Guanglong , Zhou Ye , Zhou Kui , Roy Vellaisamy A. L. , Han Su‐Ting 
Published: December 2018

AbstractGraphene oxide (GO)‐based nonvolatile memory has triggered tremendous interest owing to its high mechanical flexibility, high optical transparency, low cost fabrication, and environmental friendly manufacture for future flexible and transparent electronic devices. Although various data storage types with different switching mechanisms have been demonstrated, challenges still exist in controlling of the memory performances. Here, polyoxometalates (POMs), a type of molecular oxide cluster, is coassembled into the GO‐based memory through electrostatic layer‐by‐layer deposition approach. Assisted by the catalytic activity of POMs, the GO can be reduced to reduced graphene oxide (RGO) under UV irradiation. By this catalytic photoreduction reaction, two types of memory characteristics can be obtained including the write‐once‐read‐many‐times (WORM) derived from the GO‐based memory and the rewritable bipolar resistive switching arising from the RGO‐based counterpart. It was verified that the transition between the WORM and bipolar resistive switching is associated with the modulation of the interface barrier of POM and GO junction. Furthermore, charge trapping/detrapping process is proposed to account for the mechanism of the two memory types. This work offers valuable insight into the new design of tunable memory characteristics and emphasizes the significant role of POMs in modifying the interface features.
2D Perovskite‐Based Self‐Aligned Lateral Heterostructure Photodetectors Utilizing Vapor Deposition
Authors: Hwang Bohee , Lee Jang‐Sik 
Published: December 2018

Abstract2D organic–inorganic hybrid perovskites (OIPs) hold great promise for electronics and optoelectronics due to structural diversity, high photoluminescence, and tunable bandgap. Moreover, integrating 2D OIPs with the 2D materials including graphene, transition metal dichalcogenide, and other OIPs gives opportunities for various options in constructing new types of heterojunctions with novel functionalities such as enhanced charge transport properties or chemical stability. Here, a sequential vapor deposition method is presented to fabricate 2D OIP lateral heterostructure films (C4H9NH3)2PbI4 (BA2PbI4)–(C4H9NH3)2(CH3NH3)Pb2I7 (BA2MAPb2I7) for photodetector applications. The lateral heterostructure is realized using thermal evaporation to deposit lead iodide (PbI2) films, then exploiting vapor transport of organic vapor to form BA2PbI4, BA2MAPb2I7, and self‐aligned lateral heterostructure. A photodetector based on the lateral structure (BA2PbI4–BA2MAPb2I7) shows larger photocurrent and higher on/off ratio (>102) than those of single material‐based devices (BA2PbI4, BA2MAPb2I7). This method to fabricate 2D OIP lateral heterostructure suggests a simple way to synthesize 2D perovskite heterostructure and may open the possibility of 2D OIP perovskites to be integrated in emerging optoelectronic devices.
Chemo‐ and Regioselective Dihydroxylation of Benzene to Hydroquinone Enabled by Engineered Cytochrome P450 Monooxygenase
Authors: Zhou Hangyu , Wang Binju , Wang Fei , Yu Xiaojuan , Ma Lixin , Li Aitao , Reetz Manfred T. 
Published: December 2018

AbstractHydroquinone (HQ) is produced commercially from benzene by multi‐step Hock‐type processes with equivalent amounts of acetone as side‐product. We describe an efficient biocatalytic alternative using the cytochrome P450‐BM3 monooxygenase. Since the wildtype enzyme does not accept benzene, a semi‐rational protein engineering strategy was developed. Highly active mutants were obtained which transform benzene in a one‐pot sequence first into phenol and then regioselectively into HQ without any overoxidation. A computational study shows that the chemoselective oxidation of phenol by the P450‐BM3 variant A82F/A328F leads to the regioselective formation of an epoxide intermediate at the C3=C4 double bond, which departs from the binding pocket and then undergoes fragmentation in aqueous medium with exclusive formation of HQ. As a practical application, an E. coli designer cell system was constructed, which enables the cascade transformation of benzene into the natural product arbutin, which has anti‐inflammatory and anti‐bacterial activities.
Hydrogels based on chitosan in tissue regeneration: How do they work? A mini review
Authors: Jiao Jiao , Huang Jinjian , Zhang Zhijun 
Published: December 2018

ABSTRACTThe repair of tissue defects after injury is of significant clinical and research importance. A variety of chitosan‐based hydrogels have been investigated and applied to address this problem. By using different synthetic methods, the hydrogels exhibit distinct physical properties, including porosity, adhesion, and stiffness. These properties are considered important factors affecting cell proliferation and tissue regeneration. Meanwhile, drug delivery and cell delivery are the two main strategies to improve the therapeutic effects of chitosan‐based hydrogels, but the choices of cells or drugs are quite varied and largely depend on the specificity of the treated tissues. The present review summarizes the physicochemical properties of chitosan‐based hydrogels and their influences on cells, and lists specific ways to promote the tissue regeneration in different systems of the human body. © 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 136, 47235.
High capacity natural fiber coated conductive and electroactive composite papers electrode for energy storage applications
Authors: Masood Aneeqa , Shoukat Zahid , Yousaf Zunaira , Sana Maham , Faisal Iqbal M. , Rehman A. R. , Sultana I. , Razaq Aamir 
Published: December 2018

ABSTRACTFlexible, lightweight, and environment friendly energy storage devices are in high demand of modern disposable technology. This study presents the coating of directly collected lignocelluloses fibers from self‐growing plant, Monochoria vaginalis with conducting layers of polypyrrole and polyaniline. Fabricated paper electrodes were conductive, electroactive, all‐organic constituents, flexible, and can be cut with help of scissor in any shape. Paper electrodes were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, and thermogravimetric analysis for morphology, structural, and thermal characteristics, respectively. Furthermore, fabricated paper electrodes were characterized by cyclic voltammetry to confirm the electroactive behavior and showed excellent electrochemical performance. Paper sheets comprising lignocelluloses fibers and polypyrrole coating (LC/PPy) were employed as electrodes of symmetric cell and showed specific capacitance of 230.35 Fg−1 at current density 0.25 mA g−1 for LC/PANI, while LC/PPy showed 9.042 W h kg−1 and 91.33 W kg−1 energy density and power density, respectively. This paper electrodes are highly feasible for environmentally safe and flexible energy storage applications, particularly in era of modern disposable technology. © 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 136, 47282.
An overexpressed cytochrome P450 CYP439A1v3 confers deltamethrin resistance in Laodelphax striatellus Fallén (Hemiptera: Delphacidae)
Authors: Miah Mohammad Asaduzzaman , Elzaki Mohammed Esmail Abdalla , Husna Asmaul , Han Zhaojun 
Published: December 2018

AbstractDeltamethrin resistance in Laodelphax striatellus had been associated with its oxidative detoxification by overexpression of four cytochrome P450 monooxygenases like CYP353D1v2, CYP6FU1, CYP6AY3v2, and CYP439A1v3. The first three P450s have been validated for insecticide‐metabolizing capability and only CYP6FU1 was found to degrade deltamethrin. In this study, an investigation was conducted to confirm the capability of CYP439A1v3 to degrade deltamethrin. The CYP439A1v3 was first expressed in Sf9 cell line and its recombinant enzyme was tested for metabolic activity against different insecticides using substrate depletion assay combined with metabolite identification. Sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) and carbon monoxide (CO)‐difference spectra analysis showed that the intact cytochrome P450 protein was successfully expressed. Tests with probe substrates proved its enzyme activity, as p‐nitroanisole, ethoxycoumarin, and ethoxyresorufin were preferentially metabolized (specific activity 7.767 ± 1.22, 1.325 ± 0.37, and 0.355 ± 0.37 nmol/min per mg of protein, respectively) while only luciferin‐HEGE was not. In vitro incubation of the recombinant CYP439A1v3 protein with deltamethrin revealed hydroxylation by producing hydroxydeltamethrin. On the contrary, no metabolite/metabolism was seen with nonpyrethroid insecticide, including imidacloprid, buprofezin, chlorpyrifos, and fipronil. To the best of our knowledge, this is the first study to link a CYP450 from family 439 to confer pyrethroid resistance to L. striatellus. This finding should help in the design of appropriate insecticide resistance management for control of this strain of L. striatellus.
Low concentrations of cadmium chloride promotes protein translation and improve cell line productivity
Authors: Mahameed Mohamed , Obiedat Akram , Beck Gad , Johnson Jeffrey C. , Tirosh Boaz 
Published: December 2018

AbstractProtein translation has emerged as a critical bottleneck for overall productivity of biological molecules. An augmentation of protein translation can be achieved by cell line engineering or by sophisticated vector design. However, for industrial process development purposes, identification of media additives that promote translation will be of great value, obviating the generation of new host platforms. Here, we examined the effect of low cadmium chloride concentrations on protein synthesis and cell line productivity. At low micromolar concentrations, cadmium chloride induced the mTOR pathway and promoted total protein synthesis in HEK 293T and CHO‐K1 cells with minimal toxicity. In a parallel screening of kinase inhibitors for promoting protein expression, we identified the RSK1 inhibitor, BI‐D1870, as having a transcription promoting activity on cytomegalovirus promoter‐driven transgenes. Fed‐batch analyses of CHO‐K1 cells producing the anticoagulant factor tissue plasminogen activator (tPA) demonstrated that inclusion of cadmium chloride alone and particularly in combination with BI‐D1870 improved overall yields of tPA by more than two‐fold with minimal effect on cell growth. We, therefore, underscore the use of cadmium alone and in combination with BI‐D1870 for improving bioproduction yields.
Analysis of sialic acid levels in Chinese intravenous immunoglobulins by high‐performance liquid chromatography with fluorescence detection
Authors: Ma Li , Zhang Wei , Hou Mingxia , Li Dong , Liu Fengjuan , Du Xi , Jiang Peng , Wang Zongkui , Zhang Rong , Cao Haijun , Ye Shengliang , Li Changqing 
Published: December 2018

AbstractIntravenous immunoglobulin (IVIg) is increasingly used for the treatment of autoimmune and systemic inflammatory diseases with both licensed and off‐label indications. Recent studies indicated that IVIg‐mediated immunomodulation and anti‐inflammation are closely associated with the IgG sialylation, especially with IgG crystallizable fragment (Fc) sialylation. The sialic acid levels of the IgG molecules and Fc fragments in 12 IVIg preparations from six Chinese manufacturers were evaluated. The Fc fragments were derived from the papain digestion of IVIg, followed by affinity and size exclusion chromatography. The sialic acid levels in Fc fragments and IVIg preparations were determined by high‐performance liquid chromatography with fluorescence detection, after the sialic acid residues were released from the proteins. The results showed that the sialic acid levels in Chinese IVIg preparations ranged from 0.875 (mol/mol IgG) to 1.085 (mol/mol IgG), and the sialic acid levels in Fc fragments were from 0.321 (mol/mol Fc) to 0.361 (mol/mol Fc). Furthermore, the sialic acid levels of IVIg preparations and Fc fragments from different Chinese manufactures were significantly different. These findings will contribute to an increased understanding of Chinese IVIg preparations and the relationship between the sialic acid levels in IVIg preparations and their clinical efficacy in future clinical studies.