bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enalapril mitigates aging by targeting antioxidative genes via phosphorylated Smad1/5/9
Authors: Lyu Wencong , Wang Haochen , Du Zhehao , Wei Ran , He Jianuo , Meng Fanju , Bi Jinlong , Zhang Lijun , Zhang Chao , Guan Yiting , Tao Wei 
Published: November 2024

ABSTRACTAging increases the risk of a myriad of chronic diseases, which are expensive and difficult to treat owing to their various risk factors. Repurposing existing anti-aging medications has accelerated the development of therapies aimed at slowing aging. In this study, we revealed that enalapril, a drug widely prescribed for hypertension, can improve both cellular senescence and individual health. Mechanistically, phosphorylated Smad1/5/9 act as pivotal mediators of the anti-aging properties of enalapril. It stimulates downstream genes involved in cell cycle regulation and antioxidative defense, facilitating cell proliferation and diminishing the production of reactive oxygen species (ROS), thus increasing the antioxidative ability of enalapril. At the organismal level, enalapril has been shown to bolster the physiological performance of various organs; it notably enhances memory capacity and renal function and relieves lipid accumulation. Our work highlights the potential of enalapril to augment antioxidant defenses and combat the effects of aging, thereby indicating its promise as a treatment strategy for aging-associated diseases and its use for healthy aging.
A library of lineage-specific driver lines connects developing neuronal circuits to behavior in theDrosophilaVentral Nerve Cord
Authors: Soffers Jelly HM , Beck Erin , Sytkowski Daniel J. , Maughan Marianne E. , Devasi Devarakonda , Zhu Yi , Wilson Beth , David Chen Yu-Chieh , Erclik Ted , Truman James W. , Skeath James B. , Lacin Haluk 
Published: November 2024

AbstractUnderstanding the developmental changes in neuronal lineages is crucial to elucidate how they assemble into functional neural networks. Studies investigating nervous system development in model systems have focused on only on a few regions of the central nervous system due to the limited availability of genetic drivers that target specific neuronal lineages throughout development and adult life. This has hindered our understanding of how distinct neuronal lineages interconnect to form neuronal circuits during development. Here, we present a split-GAL4 library composed of genetic driver lines, which we generated via editing the genomic locus of lineage-specific transcription factors and demonstrate that we can use this library to specifically target most individual neuronal hemilineages in theDrosophilaventral nerve cord (VNC) throughout development and into adulthood. Using these genetic driver lines, we found striking morphological changes in neuronal processes within a lineage during metamorphosis. We also demonstrated how neurochemical features of neuronal classes can be quickly assessed. Lastly, we documented behaviors elicited in response to optogenetic activation of individual neuronal lineages and generated a comprehensive lineage-behavior map of the entire fly VNC. Looking forward, this lineage-specific split-GAL4 driver library will provide the genetic tools needed to address the questions emerging from the analysis of the recent VNC connectome and transcriptome datasets.
Transcriptional Regulation of Mammary Alveolar Proliferation and Differentiation during Early Pregnancy
Authors: Vusich John , Garcia-Lerena Jesus , Schulte Anthony , Corfixsen Ryan , Andrechek Eran 
Published: November 2024

AbstractProliferation and differentiation of the mammary gland during pregnancy is regulated by a wide variety of factors. Using gene expression data, we have predicted that the E2F5 transcription factor has a role in the mammary gland during pregnancy. Using CUT&RUN for E2F5 in combination with gene expression data revealed that there were a number of E2F5 target genes associated with gene expression in early pregnancy, suggesting a critical role. This prediction was then functionally tested through analysis of mice with an ablation of E2F5 in the mouse mammary epithelium using a Floxed E2F5 in combination with MMTV-Cre. This revealed a striking delay in alveolar proliferation and differentiation at the early stages of pregnancy. The significance of this effect was lost in both a second pregnancy and by the mid-point of pregnancy in single parous females. Analysis of E2F5 targets revealed overlap but a slight divergence from the consensus E2F sequence and that E2F5 was bound to the promoter of many genes involved in cellular proliferation, including known regulators of pregnancy associated alveolar expansion such as Stat6. However, progesterone stimulation of cells in a reporter assay revealed that it counteracts the repression of Stat6 by E2F5, leading to expression of Stat6. Together these data reveal the complexity of the transcriptional regulation of alveolar proliferation in early pregnancy.
Circuit mechanisms underlying sexually dimorphic outcomes of early life stress
Authors: Jiang Cheng , Ruiz-Sanchez Ignacio , Mei Cindy , Pittenger Christopher 
Published: November 2024

ABSTRACTStress during early life influences brain development and can affect social, motor, and emotional processes. We describe a striking sex difference in the effects of early life stress (ELS), which produces anhedonia and anxiety-like behaviors in female adolescent mice, as reported previously, but repetitive behavioral pathology and social deficits in male adolescent mice. Notably, this parallels sex differences seen in the prevalence of psychiatric symptoms: depression and anxiety disorders are more common in girls and women, whereas neurodevelopmental disorders like autism spectrum disorder and Tourette syndrome are markedly more common in boys and men. We characterized the effects of ELS on the medial prefrontal cortex (mPFC) and on its projections to the dorsal striatum (dStr) and lateral septum (LS). ELS males, but not females, developed hyperactivity in the cortico-striatal circuit and hypoactivity in the cortico-septal circuit. Chemogenetic manipulation of cortico-striatal projection neurons modulates repetitive behavioral pathology and social behaviors in stressed males, and anhedonia in stressed females. Activation of cortico-septal projection neurons rescues social deficits in stressed males. We conclude that early life stress produces sexually dimorphic behavioral effects, with potential relevance to human psychiatric symptoms, through its differential effects on cortico-striatal and cortico-septal circuits.
Non-targeted screening method for detecting temporal shifts in spectral patterns of Methicillin ResistanceStaphylococcus aureusand post-hoc description of peak features
Authors: Nichani Kapil , Uhlig Steffen , Martin Victor San , Hettwer Karina , Frost Kirstin , Steinacker Ulrike , Kaspar Heike , Gowik Petra , Kemmlein Sabine 
Published: November 2024

AbstractNon-targeted methods (NTMs) using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) show promise in bacterial resistance detection, yet temporal variations in spectral features pose significant challenges. These proteomic patterns, which characterize bacterial phenotypes and pathological functions, may vary over time due to bacterial adaptation, virulence, or resistance mechanisms, resulting in large prediction uncertainties and potentially degrading NTM performance. We present a comprehensive screening method to detect temporal changes in MALDI-TOF spectral patterns, demonstrated using methicillin-resistant and -susceptibleStaphylococcus aureus(MRSA/MSSA) isolates collected over several years. Our approach combines convolutional neural networks (CNN) with statistical methods, including significance testing, kernel density estimation, and receiver operating characteristics for dataset shift detection. We employ Gradient-weighted Class Activation Mapping (Gradcam) for post-hoc feature description, enabling biochemical characterization of temporal changes. This analysis reveals crucial insights into the dynamic relationship between spectral data patterns over time, addressing key challenges in developing robust NTMs for routine applications.
Sensing of extracellular L-Proline availability by the integrated stress response determines the outcome of cell competition
Authors: Krishnan Shruthi , Lima Ana , Low Ying Thong , Montero Salvador Perez , Tan Sizhe , Gregorio Aida Di , Barreto Adrian Perez , Bowling Sarah , Vousden Karen , Rodriguez Tristan A. 
Published: November 2024

AbstractCell competition is a quality control acting from development to the adult that eliminates cells that are less-fit than their neighbours. How winner cells induce the elimination of losers during this process is poorly understood. Here, we address this question by studying the onset of differentiation in mouse, where cell competition eliminates 35% of embryonic cells. These loser cells have mitochondrial dysfunction, and we find that this causes amino-acid deprivation and activation of the integrated stress response (ISR), a pathway essential for their survival. We show that L-Proline is a key amino-acid sensed by the ISR and that in a competitive environment, winner cells induce increased L-Proline uptake in loser cells. This causes ISR repression and their elimination. Our results imply that cell competition is acting as a nutrient sensor, eliminating dysfunctional cells when amino acids are plentiful but sparing them in nutrient poor environments.
What drives entrepreneurial intentions? Interplay between entrepreneurial education, financial support, role models and attitude towards entrepreneurship
Authors: Saoula Oussama , Abid Muhammad Farrukh , Ahmad Munawar Javed , Shamim Amjad 
Published: November 2024

Purpose
This study aims to investigate the impact of entrepreneurial education (EE), financial support (FS) and role models (RMs) on one’s attitude towards entrepreneurship (ATE) and on entrepreneurial intention (EI) based on the theory of planned behaviour.

Design/methodology/approach
In this study, quantitative research design, a deductive approach and purposive sampling have been used to collect data from 352 individuals from various Malaysian higher education institutions. The data were analysed using partial least squares structural equation modelling.

Findings
The findings have provided interesting insights into how EE, FS and RMs have a significant impact on one’s ATE and their EI. Surprisingly, RMs did not emerge as a significant predictor of EI. However, it is worth noting that mediating relationships were of significance, indicating that one’s ATE plays a substantial mediating role between EE, FS, RMs and EIs.

Originality/value
To the best of the authors’ knowledge, this study is among the few that investigate the relationships between RMs, FS and EE in relation to enhancing EIs through the mediating role of one’s ATE in Malaysia. Based on the findings of this study, stakeholders in Malaysia’s education sector are being urged to develop policy guidelines for the designing and teaching of entrepreneurship education as well as to develop FS mechanisms for business start-ups. They are also being encouraged to expose students to entrepreneurial RMs to improve their ATE and their EIs.
Impact of technology adoption on livestock production efficiency incorporating grassland productivity
Authors: Huang Yi , Huang Zhipeng , Xu Gang , Zhang Yan 
Published: November 2024

PurposeGrassland degradation is a global ecological issue that inevitably leads to low livestock production efficiency (LPE). Adoption of appropriate technology is an effective way to improve productivity. However, the rate of technology adoption among herders in less developed pastoral areas is low. Therefore, it is critical to improve the level of technology adoption in order to increase LPE.Design/methodology/approachBased on remote sensing data and survey datasets of herder households in China’s Qinghai–Xizang Plateau, this paper innovatively constructs a stochastic production frontier model incorporating grassland productivity (i.e. grassland total net primary productivity) to accurately evaluate LPE and uses fractional regression models to determine the impact of technology adoption on LPE.FindingsThe results show that grassland productivity is essential to estimating LPE, and failing to account for it will result in overestimation. Technology adopters have a technical advantage with respect to average LPE (0.596) when compared with non-adopters (0.540), and technology adoption positively contributes to LPE. Furthermore, compared with profit-seeking technology, pro-environmental technology contributes more to improving LPE, and the combined adoption of both technologies leads to a markedly greater enhancement in LPE.Originality/valueFew studies have empirically analyzed the economic benefits of technologies that most smallholders can afford, and few measure LPE considering grassland productivity. This study fills these gaps, and the findings are highly relevant for policies aimed at encouraging technology adoption and facilitating more efficient livestock production.
Do the more women on the board, the higher the firm’s performance during a COVID-19 crisis? Evidence from ASEAN countries
Authors: Nadia Linda Putri , Sudirman Wahyu Febri Ramadhan , Pratiwi Anggun 
Published: November 2024

PurposeThis study extends the literature on women directors and their impact on firm performance, focusing on developing countries and highlighting practices related to the role of gender diversity and inclusion in corporate governance, especially during a crisis like the COVID-19 pandemic.Design/methodology/approachWe analyzed unbalanced panel data with five methods: OLS, fixed effect, random effect, two-stage least squares (2SLS) and the two-step system GMM. Using the Osiris database and annual reports from five Association of Southeast Asian Nations (ASEAN) countries covering 2010 to 2021, we analyzed 2,494 director-firm-year observations and 2,823 firm-year data on 305 firms.FindingsWe found a positive link between women on the board and firm performance. More women on the board enhance performance, particularly during crises, with a significant boost when three or more women are present. However, the positive impact weakens as the number of women surpasses this threshold.Practical implicationsThis study’s finding can signal that women’s presence can bring better firm performance, although, in emerging economies, the growth of women’s presence on the board needs to be more inclusive. This condition becomes momentum for policymakers and practitioners or organizations to be more aware and count the women’s potential if they sit on the corporate board. These findings give implications and suggestions to the policymaker to make gender quotes at a high level, such as the board of directors under the critical mass theory, which is three women or one-third of the total board.Originality/valueThis research enhances understanding of how board gender diversity affects firm performance. Specifically, it examines the previously overlooked area of how diversity on boards impacts firms in developing countries facing crises.
Sanjeevani: replicating affordable child and maternal healthcare ecosystem for social transformation
Authors: Shroff Arvind , Shah Bhavin J. 
Published: November 2024

Learning outcomes
The authors have discussed the importance of creating socially transformative health-care ecosystems in emerging economies like India. After completion of this case study and assignment questions, the students will be able to apply the case experience to comprehend the underlying factors influencing the decision of the implementation and replication of the Sanjeevani health-care service delivery model, understand the impact of free and subsidized health-care service delivery models on social transformation, evaluate the operational performance of health-care institutions using the balanced scorecard model and create practical relevance when implementing novel health-care solutions like Sanjeevani, which has been successful due to its singular pin-pointed focus on solving the issue of congenital heart diseases (CHDs).

Case overview/synopsis
Forty thousand surgeries against 0.3 million new CHD patients every year was the unbalanced equation of pediatric cardiac care in India. It also contributed to almost 46% of total CHD prevalence in the world. This case study explores the evolution of the affordable health-care ecosystem provided by Sri Sathya Sai Sanjeevani Hospital (Sanjeevani), Raipur, Chhattisgarh, which included services ranging from OPD to postoperative surgical care, including accommodation and food, completely free of cost. Over the past eight years, it had managed over 80,000 pediatric cardiac outpatients and performed over 9,000 surgeries. This case study also outlines the execution of Sanjeevani, as an affordable health venture aimed at producing social transformation. The pertinent question to be explored is, “Can the Sanjeevani healthcare ecosystem be replicated, both operationally and financially?”

Complexity academic level
This case study is suited to undergraduate Bachelor of Business Administration, Master of Business Administration (MBA) and executive MBA.

Supplementary materials
Teaching notes are available for educators only.

Subject code
CSS 2: Built Environment.
Fortifying humanitarian supply chains: evaluating sustainability enablers for strengthened resilience of humanitarian supply chains during calamities and pandemics
Authors: Kashav Vishal , Garg Chandra Prakash 
Published: November 2024

Purpose
The purpose of this paper is to identify key sustainability enablers that are crucial for resilient humanitarian supply chains (RHSCs) during natural calamities and pandemics. It also aims to subsequently rank them using the fuzzy analytic hierarchy process (AHP) to assess their importance. The goal is to enhance stakeholders’ understanding, enabling them to implement effective strategies to mitigate disruptions and safeguard RHSCs.

Design/methodology/approach
This study applied the fuzzy AHP as part of the multicriteria decision-making (MCDM) framework to prioritize sustainability enablers that are crucial for the resilience of humanitarian supply chains (RHSCs).

Findings
The research findings indicate that the operational enablers category is the most critical, followed by the infrastructural and environmental categories, which rank as the second and third most critical, respectively. Among the sub-categories, “Hyper-connectivity,” “Renewable energy sources for infrastructure” and “Sustainable procurement and sourcing practices” are identified as the top three, highlighting the urgency for immediate attention.

Research limitations/implications
While this research provides valuable insights, it also presents some limitations and opens new avenues for future study. First, the study predominantly focuses on natural calamities and pandemics, potentially overlooking other catastrophic events that could jeopardize humanitarian supply chains. Second, to eliminate potential biases and develop a more robust model, it would have been beneficial to explore other modern MCDM methods. It is possible that these alternative MCDM techniques might yield better results than fuzzy AHP. Future research could explore a broader range of crises to comprehensively address the varied dynamics influencing Resilient Humanitarian Supply Chains (RHSCs) while also experimenting with different cutting-edge MCDM methods. Additionally, future research could dive deeper into the top-ranked technology enablers, particularly focusing on human-technology interactions within humanitarian supply chains, a topic that is currently being discussed among experts.

Social implications
The manuscript emphasizes the importance of global preparedness and collaborative efforts among governments, nongovernmental organizations and communities. Through its discussion of social enablers, this study aims to develop RHSCs that prioritize the well-being of disaster-affected populations and individuals used in this field.

Originality/value
This research offers unique insights by ranking sustainability enablers for resilient humanitarian supply chains (RHSCs) amid natural calamities and pandemics, providing novel and actionable contributions.
How do challenge and hindrance stressors differentially influence work-to-family enrichment? The roles of self-esteem and marital power
Authors: Tan Alice J.M. , Loi Raymond 
Published: November 2024

PurposeThe paper aims to extend the work–home resources model by identifying self-esteem as the mechanism and marital power as the moderator on the relationship between challenge/hindrance stressors and work-to-family enrichment (WFE).Design/methodology/approachThis paper conducted a three-phase paper survey. Path analytic models were used to test the hypotheses using Mplus 8.10.FindingsChallenge stressor (workload) enriches employees’ family through increased self-esteem, while hindrance stressor (role conflict) threatens WFE through decreased self-esteem. Marital power strengthens the positive relationship between self-esteem and enrichment as well as the indirect relationships between job stressors and WFE.Originality/valueThis study reveals how and when job stressors increase WFE.
Moderating the role of cybernetic controls on data accuracy and decision-making in accounting information systems in construction projects in Iraq
Authors: Al-Hashimy Hisham Noori Hussain , Yao Jinfang 
Published: November 2024

PurposeThe importance of cybernetic controls (CC) in the context of accounting information systems (AIS) in increasing data accuracy (DA) and improving decision-making (DM) has attracted much attention. In this study, the paper analyses the moderating role of CC on the relationship between DA and DM in AIS, which is supposed to be a case of construction projects in Iraq.Design/methodology/approachData were collected using a questionnaire-structured interview targeted at construction site managers and project managers, employing probability sampling on the 253 valid responses, which was analysed using the partial least squares structural equation modelling (PLS-SEM) method.FindingsCC significantly reduced the role of the moderating aspect of the DA and positively influenced DM in AIS. These results provided a base for the research postulates, including integrating CC in AIS to offer an overdue solution to challenges in construction project DM.Practical implicationsEmpirical outcomes of the study demonstrated that the implementation of CC in AIS can be applied successfully to the accounting of exact data and precision decisions in construction projects. Such lessons are strategically important to practitioners needing optimal AIS performance with a view to industry satisfaction.Originality/valueThe work has a breakthrough quality in the study at hand by stressing that CC moderate the link of DA to the DM approach in AIS. This area lacks exposition when construction projects in Iraq are the case. The findings of the research are aligned with the criteria of good governance in the context of the use of electronic systems to make decisions in AIS.