bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tissue-specific Network Analysis of Genetic Variants Associated with Coronary Artery Disease
Authors: Miao Xiao , Chen Xinlin , Xie Zhijun , Lin Honghuang 
Published: July 2018

AbstractCoronary artery disease (CAD) is a leading cause of death worldwide. Recent genome-wide association studies have identified more than one hundred susceptibility loci associated with CAD. However, the underlying mechanism of these genetic loci to CAD susceptibility is still largely unknown. We performed a tissue-specific network analysis of CAD using the summary statistics from one of the largest genome-wide association studies. Variant-level associations were summarized into gene-level associations, and a CAD-related interaction network was built using experimentally validated gene interactions and gene coexpression in coronary artery. The network contained 102 genes, of which 53 were significantly associated with CAD. Pathway enrichment analysis revealed that many genes in the network were involved in the regulation of peripheral arteries. In summary, we performed a tissue-specific network analysis and found abnormalities in the peripheral arteries might be an important pathway underlying the pathogenesis of CAD. Future functional characterization might further validate our findings and identify potential therapeutic targets for CAD.
Barlenses in the CALIFA survey: Combining photometric and stellar population analyses
Authors: Laurikainen E. , Salo H. , Laine J. , Janz J. 
Published: July 2018

Aims: It is theoretically predicted that, at low galaxy inclinations, boxy/peanut bar components have a barlens appearance of a round central component embedded in the narrow bar. We investigate barlenses in the Calar Alto Legacy Integral Field Area (CALIFA) survey galaxies, studying their morphologies, stellar populations, and metallicities. We show that, when present, barlenses account for a significant portion of light of photometric bulges, i.e., the excess light on top of the disks, which highlights the importance of bars in accumulating central galaxy mass concentrations in the cosmic timescale.Methods: We made multi-component decompositions for a sample of 46 barlens galaxies drawn from the CALIFA survey, whereM⋆/M⊙= 109.7− 1011.4andz= 0.005 − 0.03. Unsharp masks of the Sloan Digital Sky Survey (SDSS)r′-band mosaics were used to identify the boxy/peanut or X-shaped features. Barlenses are identified in the images using our simulation snapshots as an additional guide. Our decompositions with GALFIT include bulges, disks, and bars as well as barlenses as a separate component. For 26 of the decomposed galaxies the CALIFA DR2 V500 grating data cubes were used to explore stellar ages and metallicities at the regions of various structure components.Results: We find that 25 ± 2% of the 1064 galaxies in the whole CALIFA sample show either X-shaped or barlens features. In the decomposed galaxies with barlenses, on average 13% ± 2% of the total galaxy light belongs to this component, leaving less than 10% for possible separate bulge components. Most importantly, bars and barlenses are found to have similar cumulative stellar age and metallicity distributions. The metallicities in barlenses are on average near solar, but exhibit a large range. In some of the galaxies barlenses and X-shaped features appear simultaneously, in which case the bar origin of the barlens is unambiguous.Conclusion: This is the first time that a combined morphological and stellar population analysis is used to study barlenses. We show that their stars are accumulated in a prolonged time period concurrently with the evolution of the narrow bar.
Insights into the inner regions of the FU Orionis disc
Authors: Siwak Michał , Winiarski Maciej , Ogłoza Waldemar , Dróżdż Marek , Zoła Stanisław , Moffat Anthony F.J. , Stachowski Grzegorz , Rucinski Slavek M. , Cameron Chris , Matthews Jaymie M. , Weiss Werner W. , Kuschnig Rainer , Rowe Jason F. , Guenther David B. , Sasselov Dimitar 
Published: July 2018

Context. We investigate small-amplitude light variations in FU Ori occurring in timescales of days and weeks.
Aims. We seek to determine the mechanisms that lead to these light changes.
Methods. The visual light curve of FU Ori gathered by the MOST satellite continuously for 55 d in the 2013–2014 winter season and simultaneously obtained ground-based multi-colour data were compared with the results from a disc and star light synthesis model.
Results. Hotspots on the star are not responsible for the majority of observed light variations. Instead, we found that the long periodic family of 10.5–11.4 d (presumably) quasi-periods showing light variations up to 0.07 mag may arise owing to the rotational revolution of disc inhomogeneities located between 16 and 20 R⊙. The same distance is obtained by assuming that these light variations arise because of a purely Keplerian revolution of these inhomogeneities for a stellar mass of 0.7 M⊙. The short-periodic (∼3 – 1.38 d) small amplitude (∼0.01 mag) light variations show a clear sign of period shortening, similar to what was discovered in the first MOST observations of FU Ori. Our data indicate that these short-periodic oscillations may arise because of changing visibility of plasma tongues (not included in our model), revolving in the magnetospheric gap and/or likely related hotspots as well.
Conclusions. Results obtained for the long-periodic 10–11 d family of light variations appear to be roughly in line with the colour-period relation, which assumes that longer periods are produced by more external and cooler parts of the disc. Coordinated observations in a broad spectral range are still necessary to fully understand the nature of the short-periodic 1–3 d family of light variations and their period changes.
Diagnostic Value of Cardiac Magnetic Resonance Strain Analysis for Detection of Cardiac Sarcoidosis
Authors: Dabir Darius , Meyer David , Kuetting Daniel , Luetkens Julian , Homsi Rami , Pizarro Carmen , Nadal Jennifer , Thomas Daniel 
Published: July 2018

Purpose The aim of this study was to investigate the diagnostic value of cardiac magnetic resonance (CMR)-derived strain parameters in patients with sarcoidosis, and to compare results to standard CMR markers of sarcoidosis (edema/T2 ratio, early gadolinium enhancement, late gadolinium enhancement) for prediction of disease outcome.
Materials and Methods 61 patients with biopsy-proven sarcoidosis underwent CMR examination. According to CMR results, patients were divided into two different groups: patients with (CMR+; n = 23) and without (CMR–; n = 38) findings consistent with cardiac sarcoidosis. In addition, a group of healthy age-matched volunteers (controls; n = 22) served as controls. Left ventricular functional parameters as well as left ventricular longitudinal and circumferential strain were evaluated in all three groups.
Results Compared to controls, global longitudinal strain (GLS) was significantly impaired in patients with sarcoidosis, irrespective of cardiac involvement. No significant differences in GLS could be revealed between CMR+ and CMR– patients. Circumferential strain parameters were significantly impaired in CMR+ patients in comparison to CMR– patients and controls, while no significant differences could be revealed between CMR– patients and controls. GLS was significantly lower in patients with a negative outcome compared to controls. Compared to the rest of the CMR+ and CMR– patients, the GLS in patients with a negative outcome was further reduced but did not reach statistical significance.
Conclusion GLS is the only strain parameter detecting left ventricular functional impairment in sarcoidosis patients with otherwise unsuspicious CMR and controls. GLS is further reduced in patients with a negative outcome. Thus, GLS may have the potential to serve as a marker for early cardiac involvement in sarcoidosis.
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Die Hand im Fokus – Schnittstellen zwischen Chirurgie und Gefäßmedizin
Authors: Wahl Uwe , Klemenz Albrecht , Hirsch Tobias 
Published: July 2018

ZusammenfassungVibrationsbedingte Schädigungen an den Händen werden bei einer Reihe von Handwerksberufen, aber auch im Zusammenhang mit der Ausübung verschiedener Sportarten beobachtet. Knöcherne, neurogene, aber auch Gefäßläsionen können zu chronischen Beschwerden und einer dauerhaften funktionellen Beeinträchtigung führen. Aus gefäßmedizinischer Sicht sind das Hand-Arm-Vibrationssyndrom (HAVS) sowie das Hypothenar-/Thenar-Hammer-Syndrom (HHS, THS) von Interesse. Aus chirurgischer Sicht ergibt sich eine besondere Bedeutung aus der Tatsache, dass es sich bei beiden Syndromen um anerkannte Berufskrankheiten handelt (BK 2104 bzw. BK 2114), die auch im Rahmen der durchgangsärztlichen Versorgung sowie in der Begutachtung gewürdigt werden müssen. Das Hypothenar-/Thenar-Hammer-Syndrom wird ausgelöst durch wiederholte Krafteinwirkung im Bereich des Kleinfinger- oder Daumenballens und ist gekennzeichnet durch eine traumatische Läsion der A. ulnaris oder A. radialis, die zu ischämiebedingten Schmerzen im akralen Versorgungsgebiet der betroffenen Handarterien i. d. R. der dominanten Hand führt. Liegt eine erhöhte Kältesensibilität mit vasospastischen Durchblutungsstörungen infolge einer Exposition zu vibrierenden Werkzeugen und Maschinen vor, spricht man von einem Hand-Arm-Vibrationssyndrom. Die Arbeit stellt verschiedene klinische und funktionsdiagnostische Tests zur Diagnosestellung vor. Dabei kommt der doppler- und duplexsonografischen Exploration ein besonderer Stellenwert zu. Verschiedene radiologische Methoden können die Diagnostik ergänzen. Zur Behandlung der Symptome sind medikamentöse und auch physiotherapeutische Behandlungsansätze zu verfolgen, die über eine Stimulation der Vasodilatation eine Verbesserung der Perfusion bewirken sollen. Eine wirksame Kausaltherapie steht jedoch nicht zur Verfügung. Aus diesem Grund muss im Rahmen einer Begutachtung die besondere Aufmerksamkeit speziell der Anamneseerhebung mit einer zweifelsfreien Zuordnung von auslösender Noxe und Körperschaden gelten, da sich daraus für den Geschädigten sozialmedizinische bzw. rentenrelevante Konsequenzen ergeben können.
Knie-TEP-Verankerung bei Revision und schlechten Knochenverhältnissen. Knochen, Zement, Schaft, Sleeve, Konus?
Authors: Sentürk Ufuk , Perka Carsten 
Published: July 2018

ZusammenfassungNach aktuellen Registerdaten muss von einer erheblichen Steigerung von Revisionsoperationen in der Kniegelenksendoprothetik ausgegangen werden. Diese gehen häufig mit ausgeprägten knöchernen Defekten einher. Die stabile und korrekte Implantatverankerung ist und bleibt eine Herausforderung. Voraussetzung für langfristige und reproduzierbare Ergebnisse ist die Auswahl der korrekten Rekonstruktions- und Verankerungsstrategie unter Beachtung des vorliegenden Knochendefekts sowie der patientenspezifischen Anatomie.
Interventional Treatment of Acute Portal Vein Thrombosis
Authors: Wolter Karsten , Decker Georges , Kuetting Daniel , Trebicka Jonel , Manekeller Steffen , Meyer Carsten , Schild Hans , Thomas Daniel 
Published: July 2018

Background Acute portal vein thrombosis is a potentially fatal condition. In symptomatic patients not responding to systemic anticoagulation, interventional procedures have emerged as an alternative to surgery. This study sought to retrospectively evaluate initial results of interventional treatment of acute portal vein thrombosis (aPVT) using a transjugular interventional approach.
Materials and Methods Between 2014 and 2016, 11 patients were treated because of aPVT (male: 7; female: 4; mean age: 41.06 years). All patients presented a rapid onset of symptoms without collateralization of portal flow as assessed by a CT scan at the time of admittance. The patients showed thrombotic occlusion of the main portal vein (11/11), the lienal vein (10/11) and the superior mesenteric vein (10/11). Different techniques for recanalization were employed: catheter thromboaspiration (1/9), AngioJet device (7/9), local-lysis-only (1/9) and TIPSS (7/9). Local lysis was administered using a dual (4/9) or single (5/9) catheter technique. The mean follow-up was 24.32 months.
Results In 9 patients transhepatic access was successful. Initially reduction of thrombus load and recanalization were achieved in all 9 cases with residual thrombi in PV (n = 3), SMV (n = 7), and IL (n = 5). In the collective undergoing interventional procedures (n = 9) rethrombosis and continuous abdominal pain were seen in one patient, and thrombus progression after successful recanalization was seen in another. Freedom from symptoms could be achieved in 6 patients. One patient developed peritoneal and pleural effusion, respiratory insufficiency and portosystemic collaterals. Both patients who could not undergo an interventional procedure developed a cavernous transformation of the portal vein. One of them also had continuous intermittent abdominal pain.
Conclusion Interventional percutaneous approaches are able to improve patient outcome in patients with aPVT. It appears to be of utmost importance to not only remove/reduce the thrombotic material but to establish sufficient inflow and outflow by TIPS and simultaneous multi-catheter thrombolysis.
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Interaction Study between Org 10172, a Low Molecular Weight Heparinoid, and Acetylsalicylic Acid in Healthy Male Volunteers
Authors: de Boer A , Danhof M , Cohen A F , Magnani H N , Breimer D D 
Published: July 2018

SummaryPotential interactions between Org 10172 (Lomoparan®; i. v. bolus injection of 3,250 anti-Xa units followed by 750 units twice daily s. c. for 8 days) and acetylsalicylic acid (ASA) (500 mg orally 14 and 2 h before i. v. Org 10172 administration) were studied in eight healthy male volunteers using an open, randomised three-way cross-over design. Except for moderate bruising at venepuncture and s.c. injection sites which were equally distributed over all three treatments (Org 10172 alone, ASA alone, Org 10172 and ASA combined), no side effects were observed. The effects of the separate drugs on several haemostatic parameters were as expected, although the prolongations in bleeding time after ASA were highly variable and tended to be somewhat more pronounced after the combination (p >0.05). Org 10172 did not influence the inhibiting effects of ASA on platelet function nor the functional recovery afterwards, as evaluated by thromboxane A2 (TXA2) generation and collagen-induced platelet aggregation. Furthermore, no important interactions were observed with regard to the coagulation tests and plasma anti-Xa activity. Although this study did not entirely exclude small interactions between Org 10172 and ASA in this relatively small group of subjects, these effects are probably without clinical significance.
Dexamethasone and Phorbol Ester, but not Cytokines, Increase the Production of Plasminogen Activator Inhibitor Type-2 in the PL-21 Human Promyelocytic Leukemia Cell Line
Authors: Niiya Kenji , Takeuchi Tetsuo , Kobayashi Makoto , Miyoshi Isao , Hayashi Tomohiro , Sakuragawa Nobuo 
Published: July 2018

SummaryPL-21 is a promyelocytic leukemia cell line that produces plasminogen activator inhibitor 2 (PAI-2). Differentiation-linked expression of PAI-2 was investigated by adding cell-differentiation promoting agents [such as phorbol myristate acetate (PMA), retinoic acid (RA), dexamethasone (Dex), and recombinant cytokines, including tumor necrosis factor-α (TNF-a), transforming growth factor-β (TGF-β), granulocyte-colony stimulating factor (G-CSF), and interleukin-6 (IL-6)] into the culture medium of PL-21 cells. PAI-1 and PAI-2 antigens were measured by an enzyme-linked immunoassay. The PAI-1 antigen, however, became detectable only after stimulation. The presence of PAI-2 antigen was further verified by immunoblotting using a monoclonal antibody against PAI-2 purified from a PL-21 culture medium. PAI activity both in the culture medium and in the cell lysate increased approximately 70-fold after exposure to PMA. Both PAI-1 and PAI-2 antigens increased, but the amount of the latter in the culture medium and in the cell lysate was approximately 10 times and 2,500 times, as much, respectively, as that of the former. Dex also increased the intracellular PAI activity approximately 6-fold, parallel with PAI-2 antigen. PAI-1 antigen increased only slightly in the culture medium but not in the cell lysate after Dex-stimulation. As with the case of PMA, TNF-a and IL-6 induced PL-21 cells to macrophage-like cells, but did not affect the PAI activity. Thus, the increase of the PAI-2 production by PMA may not necessarily depend on differentiation into macrophages. Other cytokines examined did not increase the PAI activity. Glucocorticoid has various effects on the fibrinolytic system because it has been also shown that the mRNA of PAI-2 in a human fibrosarcoma cell line decreases in response to Dex.
Normal ADP-Induced Aggregation and Absence of Dissociation of the Membrane GP IIb-IIIa Complex of Intact Rat Platelets Pretreated with EDTA
Authors: Gachet Christian , Stierlé Anita , Ohlmann Philippe , Lanza François , Hanau Daniel , Cazenave Jean-Pierre 
Published: July 2018

SummaryADP-induced platelet aggregation requires the presence of external calcium and fibrinogen. When human platelets are incubated for 30 min at 37° C with 5mM EDTA and then resuspended in a calcium containing medium, they lose their ability to bind fibrinogen and to aggregate in response to ADP stimulation. Under these conditions, the effect of EDTA is irreversible and accompanied by dissociation of the glycoprotein (GP) IIb-IIIa complex into its free subunits, GP IIb and GP IIIa. We studied the effect of incubation of intact rat platelets with 5 mM EDTA at 37° C from 30 to 120 min. EDTA treated rat platelets showed normal aggregation in response to 5 εM ADP in the presence of added purified rat fibrinogen and bound 125I-labeled rat fibrinogen at the same rate and magnitude after stimulation with 5 εM ADP as untreated platelets. Control and EDTA treated rat platelets, labeled or not with 125I and solubilized in Triton X-100, had a similar pattern of immunoprecipitates after crossed immunoelectrophoresis (CIE) analysis. The rat GP IIb-IIIa arc was located by incorporation of an 125I-labeled polyclonal anti-human GP IIb-IIIa antibody. In contrast, in experiments using rat platelet lysates, we demonstrated that the rat GP IIb-IIIa is a Ca2+-dependent heterodimer as it was dissociated by EDTA. Using SDS-PAGE and two-dimensional SDS-PAGE, the rat GP IIb-IIIa complex was found to have characteristics similar to the human complex with the exception that the light chain of the rat GP IIb was undetectable after 125I surface labeling. Small structural particularities of rat GP IIb-IIIa might still explain the functional differences observed between intact rat and human platelets treated with EDTA.
Factor IXa Inhibition Contributes to the Heparin Effect
Authors: Béguin Suzette , Dol Frédérique , Hemker H Coenraad 
Published: July 2018

SummaryWe investigated whether the inactivation of factor IXa contributes to the partial inhibition of thrombin formation that is observed at therapeutic concentrations of heparin. The action of standard unfractionated heparin (0.05 U/ml) on thrombin formation in the intrinsic system was compared to that of a mixture of dermatan sulfate (DS) and a synthetic pentasaccharide (PS). DS enhances the action of heparin cofactor II which inhibits thrombin only. PS specifically enhances the anti-factor Xa activity of antithrombin III (AT III). The concentrations of DS and PS were chosen so as to obtain equal anti-thrombin and anti-factor Xa activities as in 0.05 U/ml heparin. An extra inhibitory effect of heparin over the mixture is observed in situations where free factor IXa, not bound to factor VIIIa and phospholipid, limits the rate of thrombin formation, notably in contact activated plasma. We conclude that the inactivation of free factor IXa by heparin contributes importantly to the inhibition of thrombin formation in the intrinsic system such as e.g. measured in the activated partial thromboplastin time.
Dipyridamole Potentiates Platelet Inhibition by Nitric Oxide
Authors: Bult Hidde , Fret Hermine R L , Jordaens François H , Herman Arnold G 
Published: July 2018

SummaryIn a placebo-controlled double blind cross-over experiment the adenosine uptake inhibitor dipyridamole (400 mg/day) did not affect ex vivo platelet aggregation induced by collagen or adenosine-diphosphate (ADP) in an electronic whole blood aggregometer (WBA). Dipyridamole was also inactive in vitro, unless red blood cell injury was deliberately enhanced, thereby increasing the level of free adenine nucleotides. Since dipyridamole also inhibits cyclic guanosine monophosphate (GMP) phosphodiesterase (PDE), we used platelet rich plasma (PRP) to study its interaction with authentic and endothelium-derived nitric oxide (NO). The latter inhibits platelets by increasing cyclic GMP. Dipyridamole (1 to 30 εM), either alone or in combination with a subthreshold concentration of prostacyclin (PGI2), was inactive. However, when combined with a subthreshold concentration of NO, dipyridamole caused a concentration-dependent platelet suppression, which became more pronounced when PGI2 was present as well. It is concluded that dipyridamole could reduce the threshold for platelet suppression by NO through inhibition of cyclic GMP PDE.
Characterization of Epitheloid Cells from Human Omentum: Comparison with Endothelial Cells from Umbilical Veins
Authors: Latron Y , Alessi M C , George F , Anfosso F , Poncelet P , Juhan-Vague I 
Published: July 2018

SummaryCapillary cells represent 95% of the vascular bed, and cells from large and micro-vessels do not express identical functions. In order to study the hormonal regulation of plasminogen activator inhibitor 1 (PAI-1) secretion by human capillary cells we used epithelial cells from omental tissue (HOTMEC). As their endothelial origin is subject to controversy, we attempted to determine their characteristics by comparing them to human umbilical vein endothelial cells (HUVEC). Morphological and biological criteria were studied. By phase contrast microscopy HOTMEC elicited a cobblestone pattern similar to HUVEC. Weibel-Palade bodies were not found in the cytoplasm with electron microscopy. Fluorescence microscopy studies indicated that HOTMEC took up acetylated-LDL more intensely than HUVEC, and showed no staining for von Willebrand factor. The phenotype of HOTMEC was studied by flow cytometry using monoclonal antibodies (mo Ab) directed against epitopes either specific for endothelial cells or for mesothelial cells. We showed that in our preparations only 10% of cells reacted with mo Ab specific for endothelial cells. About 60% of the HOTMEC were labelled with an antibody directed against mesothelial cells. HOTMEC expressed fibrinolytic factors. Tissue plasminogen activator (t-PA) levels in HOTMEC conditioned medium were 50 fold higher than those of HUVEC, and the PAI-1 secretions were identical in both cell types. Insulin which is known to increase PAI-1 synthesis by hepatocytes did not enhance the PAI-1 level either in HOTMEC or in HUVEC conditioned media. Our results suggested that morphological and functional methods did not allow discrimination between the cell types present in the omentum tissue. They also showed that the population obtained from the omental tissue by collagenase digestion is heterogeneous, with few cells expressing endothelial markers.