bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of crab shell extract as a coagulant on the textural and sensorial properties of tofu (soybean curd)
Authors: Jun Joon‐Young , Jung Min‐Jeong , Jeong In‐Hak , Kim Gwang‐Woo , Sim Jae‐Man , Nam Si‐Youl , Kim Byoung‐Mok 
Published: January 2019

AbstractTo find an economic use of red snow crab (Chionoecetes japonicus) shell waste, we focused on its high mineral content. To evaluate its usability as a coagulant for tofu making, the effects of the crab shell extracts on the textural and sensorial properties of the tofu samples were investigated. The crab shell powder (CSP) and ash (CSA) were used for their extract preparation, and 1%–5% acetic acid treatment led to an abundance of calcium in the resulting extracts. The tofu yields of all the acetic acid extracts were comparable with those of the commercial coagulants MgCl2 and glucono‐δ‐lactone (GDL). Furthermore, the results for the textural attributes and sensorial acceptability demonstrated that either the extract from CSP prepared with 3% acetic acid or the extracts from CSA prepared with 1% or 3% acetic acid could be used as coagulants, because all the values of the extracts were statistically equivalent to those of the MgCl2 and GDL (p < 0.05).
Depth of invasion in patients with early stage oral cancer staged by sentinel node biopsy
Authors: den Toom Inne J. , Janssen Luuk M. , van Es Robert J.J. , Karagozoglu K. Hakki , de Keizer Bart , van Weert Stijn , Willems Stefan M. , Bloemena Elisabeth , Leemans C. René , de Bree Remco 
Published: January 2019

AbstractBackgroundTo investigate if depth of invasion (DOI) can predict occult nodal disease in patients with cT1‐2N0 (7th TNM) oral squamous cell carcinoma (OSCC) staged by sentinel lymph node biopsy (SLNB).MethodsIn 199 OSCC patients, DOI measurements and SLNB were performed.ResultsMetastases were found in 64 of 199 patients (32%). Of these 64 patients, the mean DOI was 6.6 mm compared to 4.7 mm in patients without metastases (P = .003). The ROC‐curve showed an area under the curve of 0.65 with a most optimal cutoff point of 3.4 mm DOI (sensitivity 83% and specificity 47%). Regional metastases were found in 15% of patients with DOI ≤ 3.4 mm.ConclusionDOI seems to be a poor predictor for regional metastasis in patients with cT1‐2N0 OSCC. Therefore, staging of the neck using SLNB in patients with early stage oral cancer should also be performed in tumors with limited DOI and probably in T3 (8th TNM) OSCC ≤4 cm diameter.
Burden of Primary Biliary Cholangitis Among Inpatient Population in the United States
Authors: Shahab Omer , Sayiner Mehmet , Paik James , Felix Sean , Golabi Pegah , Younossi Zobair M. 
Published: January 2019

Primary biliary cholangitis (PBC) is an autoimmune liver disease that can lead to cirrhosis and liver failure. Our aim was to assess the recent trends in the mortality rates and health care utilization of patients with PBC seen in the inpatient setting in the United States. We used the National (Nationwide) Inpatient Sample data (2005‐2014). The study population included adults with PBC, using International Classification of Diseases, Ninth Revision codes. Trends in PBC‐related discharges, total charges, length of stay (LoS), and in‐hospital mortality were evaluated. Hierarchical generalized linear models were performed for determining predictors of mortality and total hospital charges. Between the study years of 2005 and 2014, a total of 22,665 hospitalized cases with PBC were identified (mean age 63 years; 84% female, 76% white). The number of PBC‐related discharges increased from 3.24 per 100,000 in 2005 to 3.68 per 100,000 in 2014, with an average annual increase of 1.4% (95% confidence interval [CI]: 0.4%‐2.4%). Fifty‐seven percent had Medicare as their primary payer, 37% had cirrhosis, and 1.3% had hepatocellular carcinoma. Between 2005 and 2014, the average total charges for PBC increased from $53,901 to $57,613 (annual percent change [APC], 1.7%; 95% CI: −0.2%‐3.5%), LoS decreased from 6.9 days to 5.4 days (APC, −2.2%; 95% CI: −3.2% to −1.1%), and mortality rate decreased from 3.8% to 2.8% (APC, −5.4%; 95% CI: −8.4% to −2.4%). Multivariable analysis revealed that ascites were independently associated with increased risk of in‐hospital mortality (odds ratio: 1.77; 95% CI: 1.50‐2.08), increased charge (percent change: 22.5%; 95% CI: 18.6%‐26.7%), and increased LoS (percent change: 29.7%; 95% CI: 25.7%‐33.9%). Conclusion: The number of PBC cases has increased in recent years. Mortality and LoS have decreased, and the total charges have remained the same.
Anticancer effect of novel platinum nanocomposite beads on oral squamous cell carcinoma cells
Authors: Tanaka Mai , Okinaga Toshinori , Iwanaga Kenjiro , Matsuo Kou , Toyono Takashi , Sasaguri Masaaki , Ariyoshi Wataru , Tominaga Kazuhiro , Enomoto Yasushi , Matsumura Yasufumi , Nishihara Tatsuji 
Published: January 2019

AbstractNanoparticles are used in industry and medicine, because of their physiochemical properties, such as size, charge, large surface area and surface reactivity. Recently, metal nanoparticles were reported to show cell toxicity on cancer cells. In this study, we focused novel platinum nanoparticles‐conjugated latex beads (P2VPs), platinum nanocomposite (PtNCP) beads, and investigated the possibility to incorporate novel anti‐cancer effect of these combined nanoparticles. Oral squamous cell carcinoma cell lines, HSC‐3‐M3 cells were injected subcutaneously into the back of nude mice to produce a xenograft model. PtNCP beads were injected locally and examined by measuring tumor volume and comparing pathological histology. PtNCP beads treatment suppressed tumor growth and identified increasing pathological necrotic areas, in vivo. PtNCP beads inhibited the cell viability of HSC‐3‐M3 cells in dose‐dependent manner and induced the cytotoxicity with extracellular LDH value, in vitro. Furthermore, SEM images were morphologically observed in PtNCP beads‐treated HSC‐3‐M3 cells. The aggregation of the PtNCP beads on the cell membrane, the destructions of the cell membrane and globular structures were observed in the SEM image. Our results indicated that a potential anti‐cancer effect of the PtNCP beads, suggesting the possibility as a therapeutic tool for cancer cell‐targeted therapy. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 2281–2287, 2019.
Rapid optical tissue clearing using poly(acrylamide‐co‐styrenesulfonate) hydrogels for three‐dimensional imaging
Authors: Ono Yuta , Nakase Ikuhiko , Matsumoto Akikazu , Kojima Chie 
Published: January 2019

AbstractOptical tissue clearing methods are attractive for three‐dimensional imaging of intact tissues and organs. A CLARITY (clear lipid‐exchanged acrylamide‐hybridized rigid imaging/immunostaining/in situ‐hybridization‐compatible tissue‐hydrogel) method using polyacrylamide gels was recently developed. In the current study, we used poly(acrylamide‐co‐styrenesulfonate) gels as a polyelectrolyte gel for a passive CLARITY method. First, radical copolymerization of acrylamide (AAm) and sodium p‐styrenesulfonate (SS) at different ratios was performed. The composition of the copolymer could be controlled by changing the monomer ratio. With increased SS content, the molecular weight decreased and the refractive index slightly increased. The poly(AAm‐co‐SS) hydrogels with a higher SS content were more swollen and looser. The clearing time could be reduced using the poly(AAm‐co‐SS) hydrogel with a higher SS content. Three‐dimensional fluorescence imaging of the poly(AAm‐co‐SS) hydrogel‐cleared tissues was successfully performed after treatment using a blue fluorescent DNA stain. Therefore, the use of the poly(AAm‐co‐SS) hydrogel improved the clearing process in the passive CLARITY method, and the technique is useful for fluorescence imaging of DNA. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 2297–2304, 2019.
Design of novel organic–inorganic composite bone cements with high compressive strength, in vitro bioactivity and cytocompatibility
Authors: Ji Mizhi , Ding Zhengwen , Chen Hong , Peng Haitao , Yan Yonggang 
Published: January 2019

AbstractIn this work, novel bioactive organic–inorganic composite bone cements consisting of tricalcium silicate (C3S), sodium alginate (SA), and calcium sulfate hemihydrate (CS) were successfully fabricated for the first time via a special method designing material composition and internal structure simultaneously, which was intended to enhance mechanical performance by combining progressive hydration process of C3S with distinctive gelation capacity of SA and further improve degradability and self‐setting properties with the addition of CS. Depending on the synergistic combination of hydration and gelation, the C3S/SA/CS composite cements (45/45/10 wt %) obtained extremely higher compressive strength up to 92.41 MPa as compared with each single component. The reinforcing mechanisms involving interfacial interaction and interior microstructure were proposed to explain this enhancement phenomenon. Additionally, the final setting time could be reduced from 68 min to 21 min with the increasing CS content. The composite cements possessed good apatite mineralization ability in simulated body fluid solution and moderate degradation rate in phosphate buffer solution. What's more, the composite cements exhibited excellent cytocompatibility and increased proliferation of rat bone‐marrow stem cells. This study could provide guidelines for the preparation of bioactive composite cements with enhanced mechanical performance, which may be suitable for load‐bearing bone repair. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 2365–2377, 2019.
Adsorptive filtration of As(III) from drinking water by CuFe2O4 particles embedded in carbon nanotube membranes
Authors: Luan Hongyan , Xu Jingwen , Tan Zhiqiang , Zheng Aihua , Huang Haiou 
Published: January 2019

AbstractBACKGROUNDFor effective removal of arsenite [As(III)] from drinking water, copper ferrite (CuFe2O4) particles were prepared by using a hydrothermal method and incorporated into layered carbon nanotube (CNT) membranes. The prepared composite membranes were used in the filtration of As(III) under conditions representative of drinking water treatment.RESULTSThe results demonstrated that the CNT membrane successfully immobilized CuFe2O4 particles and prevented their release into the filtered water. Moreover, the composite membrane consistently removed >90% of As(III) under various solution chemical conditions. Besides, the CuFe2O4/CNT membrane was readily regenerated with 0.001 mol L–1 NaOH solution, allowing repeated and sustained use of the membrane for As(III) removal. However, the presence of phosphate or high concentration of natural organic matter (NOM) suppressed arsenic (As) removal, which necessitates consideration of feedwater properties in the application of the composite membrane.CONCLUSIONSThis study developed a facile method for the integration of CuFe2O4 particles with a membrane filtration technique for efficient As decontamination. The novel composite membranes have a promising potential for As(III) removal from drinking water. © 2019 Society of Chemical Industry
Environmental sustainability assessment of renewables‐based propylene glycol at full industrial scale production
Authors: Nachtergaele Pieter , De Meester Steven , Dewulf Jo 
Published: January 2019

AbstractBACKGROUNDGlycerol, a by‐product from oleochemical or biodiesel production, can be used to produce renewables‐based propylene glycol (PG). The environmental sustainability of PG was evaluated using life‐cycle assessment. The effect of shifting from petrochemical to renewables‐based PG was assessed by using data from an industrial scale renewables‐based PG production plant. A contribution analysis was performed to identify the hotspots in the production process. The effects of implementing a sustainable sourcing strategy and producing steam/electricity by combined heat and power (CHP) were assessed.RESULTSA switch from petrochemical to renewables‐based PG results in a reduction of the climate change impact between 40% and 60% kg CO2 eq. A shift of burden is found to agriculture‐related indicators. For the biodiesel and oleochemical production route, respectively, 73% and 59% of the impact on climate change is related to feedstock production. By implementing two improvements, the emitted CO2 eq. for biodiesel PG could be reduced by 38%.CONCLUSIONSSwitching from petrochemical to renewables‐based PG has a clear environmental benefit for slowing climate change. However, the shift of burden towards agriculture‐related indicators shows the need to ensure sustainable agricultural practices. Combining both on‐site improvements and a more sustainable supply chain effectively decreases the environmental impact. © 2019 Society of Chemical Industry
Three‐dimensional echocardiography of mitral Barlow's disease with infective endocarditis: Perforations or cleft‐like indentations?
Authors: Pang Bo , Wang Yonghuai , Liu Shuang , Yang Jun , Gu Tianxiang , Ma Chunyan 
Published: January 2019

AbstractBarlow's disease is a complicated form of degenerative mitral valve (MV) disease. Infective endocarditis (IE) often occurs on the basis of primary heart diseases and may be combined with valve perforations. Cleft‐like indentations (CLIs) were suggested by Ring et al. in 2013. They are located at the inter‐scallop position and involve at least one‐half of the valve. Herein, we report a case of Barlow's disease combined with IE and CLIs, which was confirmed intra‐operatively and by histopathological examination. The CLIs were misdiagnosed by two‐dimensional transthoracic echocardiography as perforations, but rightly interpreted by preoperative three‐dimensional echocardiography. The possibility of CLIs should be considered in the evaluation of mitral regurgitation caused by myxomatous MV diseases.
Clinical, radiological, and genetic characteristics of 16 patients with ACO2 gene defects: Delineation of an emerging neurometabolic syndrome
Authors: Sharkia Rajech , Wierenga Klaas J. , Kessel Amit , Azem Abdussalam , Bertini Enrico , Carrozzo Rosalba , Torraco Alessandra , Goffrini Paola , Ceccatelli Berti Camilla , McCormick M. Eileen , Plecko Barbara , Klein Andrea , Abela Lucia , Hengel Holger , Schöls Ludger , Shalev Stavit , Khayat Morad , Mahajnah Muhammad , Spiegel Ronen 
Published: January 2019

AbstractMitochondrial aconitase is the second enzyme in the tricarboxylic acid (TCA) cycle catalyzing the interconversion of citrate into isocitrate and encoded by the nuclear gene ACO2. A homozygous pathogenic variant in the ACO2 gene was initially described in 2012 resulting in a novel disorder termed “infantile cerebellar retinal degeneration” (ICRD, OMIM#614559). Subsequently, additional studies reported patients with pathogenic ACO2 variants, further expanding the genetic and clinical spectrum of this disorder to include milder and later onset manifestations. Here, we report an international multicenter cohort of 16 patients (of whom 7 are newly diagnosed) with biallelic pathogenic variants in ACO2 gene. Most patients present in early infancy with severe truncal hypotonia, truncal ataxia, variable seizures, evolving microcephaly, and ophthalmological abnormalities of which the most dominant are esotropia and optic atrophy with later development of retinal dystrophy. Most patients remain nonambulatory and do no acquire any language, but a subgroup of patients share a more favorable course. Brain magnetic resonance imaging (MRI) is typically normal within the first months but global atrophy gradually develops affecting predominantly the cerebellum. Ten of our patients were homozygous to the previously reported c.336C>G founder mutation while the other six patients were all compound heterozygotes displaying 10 novel mutations of whom 2 were nonsense predicting a deleterious effect on enzyme function. Structural protein modeling predicted significant impairment in aconitase substrate binding in the additional missense mutations. This study provides the most extensive cohort of patients and further delineates the clinical, radiological, biochemical, and molecular features of ACO2 deficiency.
Tissue-type plasminogen activator neutralizes LPS but not protease-activated receptor-mediated inflammatory responses to plasmin
Authors: Zalfa Cristina , Azmoon Pardis , Mantuano Elisabetta , Gonias Steven L 
Published: January 2019

Abstract
Tissue-type plasminogen activator (tPA) activates fibrinolysis and also suppresses innate immune system responses to LPS in bone marrow-derived macrophages (BMDMs) and in vivo in mice. The objective of this study was to assess the activity of tPA as a regulator of macrophage physiology in the presence of plasmin. Enzymatically active and enzymatically inactive (EI) tPA appeared to comprehensively block the response to LPS in BMDMs, including expression of proinflammatory cytokines such as TNF-α and IL-1β and anti-inflammatory cytokines such as IL-10 and IL-1 receptor antagonist. The activity of EI-tPA as an LPS response modifier was conserved in the presence of plasminogen. By contrast, in BMDMs treated with tPA and plasminogen or preactivated plasmin, in the presence or absence of LPS, increased proinflammatory cytokine expression was observed and tPA failed to reverse the response. Plasmin independently activated NF-κB, ERK1/2, c-Jun N-terminal kinase, and p38 mitogen-activated protein kinase in BMDMs, which is characteristic of proinflammatory stimuli. Plasmin-induced cytokine expression was blocked by ε-aminocaproic acid, aprotinin, and inhibitors of the known plasmin substrate, Protease-activated receptor-1 (PAR-1), but not by N-methyl-d-aspartate receptor inhibitor, which blocks the effects of tPA on macrophages. Cytokine expression by BMDMs treated with the PAR-1 agonist, TFLLR, was not inhibited by EI-tPA, possibly explaining why EI-tPA does not inhibit macrophage responses to plasmin and providing evidence for specificity in the ability of tPA to oppose proinflammatory stimuli. Regulation of innate immunity by the fibrinolysis system may reflect the nature of the stimulus and a balance between the potentially opposing activities of tPA and plasmin.
Persistent systemic rotavirus vaccine infection in a child with X‐linked severe combined immunodeficiency
Authors: Yoshikawa Tetsushi , Ihira Masaru , Higashimoto Yuki , Hattori Fumihiko , Miura Hiroki , Sugata Ken , Komoto Satoshi , Taniguchi Koki , Iguchi Akihiro , Yamada Masafumi , Ariga Tadashi 
Published: January 2019

AbstractObjectiveThe main aims of the present study were to elucidate the systemic group A rotavirus (RVA) infection and to clarify the genetic changes of persistent virus in the X‐linked severe combined immunodeficiency (SCID) patient.MethodsRotaTeq vaccine (RV5) genotype‐specific real‐time reverse transcription polymerase chain reaction was used to monitor viral RNA load in serially collected serum and stool samples. Next‐generation sequence analysis was used to determine the genotype of the virus by sequencing 11 gene segments. Polyacrylamide gel electrophoresis (PAGE) analysis was used to identify rearrangement of viral genes. The gene rearrangement was examined in NSP5 gene by using Sanger sequence.ResultsA 7‐month‐old boy demonstrated chronic diarrhea following the third administration of RV5 and failure to thrive. He was diagnosed with X‐linked SCID and successfully underwent cord blood transplantation. High copy numbers of RV5 genotype G1 RNA were detected in serially collected stool and serum samples and the kinetics of viral RNA loads were correlated with the degree of clinical disease. Next‐generation sequence analysis revealed genetic reassortment at least between the strains WI79‐9/G1P7[5] and WI79‐4/G6P1A[8] in the VP7 gene and the VP4 gene among the vaccine‐derived rotavirus strains. In addition, PAGE analysis suggested genetic rearrangements in several genes, and it was confirmed in the NSP5 gene by sequence analysis.ConclusionsThe kinetics of RVA RNA load in serum and stool samples was consistent with the clinical course of the patient. Among five genotypes of RV5 vaccine, G1 genotype replicated well in this patient. Reassortment and rearrangements were demonstrated in persistently infected G1 genotype of RV5.
Periodontal disease may induce liver fibrosis in an experimental study on Wistar rats
Authors: Mester Alexandru , Ciobanu Lidia , Taulescu Marian , Apostu Dragos , Lucaciu Ondine , Filip Gabriela Adriana , Feldrihan Vasile , Licarete Emilia , Ilea Aranka , Piciu Andra , Oltean‐Dan Daniel , Scurtu Iuliu , Berce Cristian , Campian Radu Septimiu 
Published: January 2019

AbstractBackgroundThe aim was to assess the effects of periodontal disease in promoting liver fibrosis in a rat model of ligature‐induced periodontitis.MethodsTwenty‐four Wistar rats were divided into four groups: control (CTRL), experimental periodontitis group at day 7 (PER7), at day 14 (PER14), at day 21 (PER21). Experimental periodontitis was induced by the placement of a silk ligature around mandibular incisors. The following parameters were assessed: gingival index, tooth mobility; liver status, and portal vein caliber by ultrasound examination; bone retraction, bone mineral density (BMD), bone volume/tissue volume (BV/TV) by micro‐CT analysis; aspartate aminotransferase (ASAT) and alanine aminotransferase (ALAT); oxidative stress (malondialdehyde [MDA], reduced glutathione/oxidative glutathione ratio [GSH/GSSG]), and matrix metalloproteinase‐8 (MMP‐8) levels; and histopathological evaluation of periodontal and liver tissues.ResultsPeriodontal parameters showed the development of periodontitis in experimental groups. Micro‐CT results indicates an increase of bone retraction and BMD values and a decrease of BV/TV value in PER groups. Liver fibrosis could not be diagnosed with ultrasound examination in any of the groups. Elevated levels of ASAT and ALAT in PER groups compared with CTRL group were found. MDA have indicated elevated levels and a decrease of GSH/GSSG ratio in PER group compared with the CTRL group. Levels of MMP‐8 have indicated high values in PER21 compared with the other groups. Histological analysis of the periodontal and liver tissues sustains the link between periodontal and hepatic injury.ConclusionThis study demonstrates a positive correlation between periodontal lesions and liver disease. Periodontitis may be an independent risk factor for liver fibrosis.
Posttraumatic Stress Symptoms and Disorders in Parents of Children and Adolescents With Chronic Physical Illnesses: A Meta‐Analysis
Authors: Pinquart Martin 
Published: January 2019

AbstractThe present meta‐analysis analyzed whether parents of young people with pediatric chronic physical illnesses experience higher levels of posttraumatic stress symptoms (PTSS) and higher rates of posttraumatic stress disorder (PTSD) than other adults and searched for correlates of PTSS in these parents. Based on a systematic search in electronic databases, 184 studies were included in the meta‐analysis. On average, 18.9% of the parents fulfilled the criteria for PTSD, and PTSS were more common among parents of young people with pediatric chronic physical illnesses than in other adults, with Hedges' g showing a 0.85 increase in standard deviation units. Parental PTSS were most prevalent among parents of children with epilepsy, g = 1.25, and diabetes, g = 1.16, and were positively associated with being the mother, r = .19; illness severity, r = .18; treatment duration/intensity, r = .21; and PTSS of the child, r = .34. In contrast, longer illness duration, r = −.19; longer time since active treatment, r = −.10; and better social resources, rs = −.17 to −.07, were associated with lower parental PTSS. The findings indicate that parents who have faced traumatic events in the context of their child's chronic illnesses should be screened for PTSS and PTSD and receive psychological interventions when needed.