bluuebunny/crossref_metadata_2025_split huggingface dataset:
Circulating kidney injury molecule‐1 as a biomarker of renal parameters in diabetic kidney disease
Authors: Gohda Tomohito , Kamei Nozomu , Koshida Takeo , Kubota Mitsunobu , Tanaka Kanako , Yamashita Yoshinori , Adachi Eri , Ichikawa Saki , Murakoshi Maki , Ueda Seiji , Suzuki Yusuke 
Published: September 2019

AbstractAims/IntroductionUrinary kidney injury molecule‐1 (KIM‐1) has been associated with proximal tubular damage in human and animal studies. Although it has been recognized as a biomarker of acute kidney injury and chronic kidney disease, its significance in the serum remains unclear. Therefore, we examined the relationship of serum and urinary KIM‐1 levels with renal parameters in patients with type 2 diabetes.Materials and MethodsSerum and urinary KIM‐1 levels, together with urinary liver‐type fatty acid‐binding protein, were measured in 602 patients with type 2 diabetes and an estimated glomerular filtration rate (eGFR) ≥30 mL/min/1.73 m2. These were then compared with the urinary albumin‐to‐creatinine ratio and eGFR.ResultsThe serum and urinary KIM‐1 levels were significantly different among the three (eGFR ≥60, 45–59, <45 mL/min/1.73 m2) groups. These levels were positively associated with the albumin‐to‐creatinine ratio and negatively associated with eGFR. In a multivariate logistic model, both serum and urinary KIM‐1 were associated with an increased albumin‐to‐creatinine ratio (>30 mg/g Cr), but only the serum KIM‐1 was associated with a lower eGFR (<60 mL/min/1.73 m2), after adjustment for covariates.ConclusionsRenal parameters appear to be strongly associated with serum KIM‐1, and not urinary KIM‐1, in patients with type 2 diabetes and an eGFR ≥30 mL/min/1.73 m2.
Parasitism of Hypothenemus hampei (Coleoptera: Scolytidae) in different farming systems and altitudes of Mount Elgon, Uganda
Authors: Ijala Anthony Raphael , Kyamanywa Samuel , Cherukut Scola , Sebatta Christopher , Karungi Jeninah 
Published: September 2019

AbstractAltitude and farming system play a vital role in modifying the niche for arthropods, by directly influencing microclimatic conditions, the quality and quantity of vegetative cover, which act variably on the behaviour of the pests, and their natural enemies. The objective of the study was to determine their effect on the abundance and parasitism of the Coffee berry borer (Hypothenemus hampei) in the Mount Elgon region. Altitude was categorized as: low (1,400–1,499 m.a.s.l); mid (1,500‐1,679 m.a.s.l); and high (1,680–2,100 m.a.s.l), and farming system was categorized as: Coffee monocrop; Coffee + annual; Coffee + banana; and Coffee + banana + shade trees. For each altitudinal range, each farming system was represented three times. The study was in two districts of the Mt. Elgon, covering a total of 72 Arabica Coffee study sites. The work involved field pest infestation inventories, followed by laboratory rearing for the abundance and parasitism studies. The results revealed highly significant interactions between altitude and farming system in influencing the abundance of the pest and its four parasitoids: Phymastichus coffea, Cephalonomia stephanoderis, Prorops nasuta and Heterospilus coffeicola. C. stephanoderis was highest in the mid‐altitudes within Coffee + banana+shade tree system; P. nasuta was highest at high altitude within Coffee + banana system; P. coffea was most abundant at mid‐altitude within Coffee + banana system, whereas H. coffeicola was highest at high altitude within the Coffee + annual cropping system. H. hampei counts were highest at low altitudes, especially in the Coffee + annual system. Some of these trends can be explained by the condition of the microclimate in the Coffee fields. There was a negative relationship between temperature and abundance of all the four parasitoids. Only C. stephanoderis had a relationship (+) with semi‐natural vegetation species counts. And only H. coffeicola had a relationship (+) with light intensity. These contrasted with H. hampei, which was positively related to temperature and negatively to light intensity.
The long‐term impact of an interprofessional humanistic faculty development programme: A qualitative investigation
Authors: Tortez Leanne M. , Quinlan Phyllis S. , Makaryus Amgad N. , George Cicy , Caruso Vincenza , Gilman Sarah , Ricardo Alison , Fornari Alice 
Published: September 2019

AbstractRationale, aims, and objectivesWhile it has long been supported that faculty development programmes serve as a means to improving practical knowledge, professional skills, and identity formation for faculty, significantly less research is focused on how learning that occurs in faculty development programmes is actually employed in the workplace and ingrained in day‐to‐day activities. The present study qualitatively explored the long‐term impact of the Mentoring and Professionalism in Training (MAP‐IT) programme, a longitudinal, interprofessional faculty development curriculum designed to enhance clinicians' humanistic mentoring skills, specifically nurses and physicians.MethodParticipants included 21 former high‐potential mentors and facilitator leaders who had graduated from the MAP‐IT programme from 2014 to 2016. Semi‐structured focus groups and interviews were conducted between August and September of 2017 to collect participant experiences of the impact of MAP‐IT skills on their professional roles (with colleagues and patients) in their clinical environments. Qualitative data were analysed using content analysis methodology.ResultsQualitative analyses using an editing analysis style resulted in nine major themes, including incorporation into clinical practice, self‐care, team building and conflict resolution, mindfulness, mentorship, professionalism, interprofessional collaboration, humanism, and appreciative inquiry.ConclusionThe personal and professional development instilled through the MAP‐IT programme was found to remain important over time, years after participation in the programme had concluded, supporting its “durability.” Implications are also discussed.
Polymetamorphism in high‐T metamorphic rocks: An example from the central Appalachians
Authors: Broadwell Kirkland S. , Caddick Mark J. , Beard James S. 
Published: September 2019

AbstractContact metamorphism associated with mafic intrusives is one of several mechanisms that has been invoked to produce extensive high‐temperature (HT) metamorphism and associated partial melting of the crust. Indisputable evidence for polymetamorphism in these settings can be difficult to decipher because both melt loss and retrogression (i.e. rehydration) can erase or obscure the records of earlier HT metamorphism by modifying HT mineral parageneses and compositions. Here, a combination of detailed field and petrographical observations, inverse mineral thermometry, and thermodynamic forward modelling is used to delineate the polymetamorphic history of migmatites from the Smith River Allochthon (SRA) in the central Appalachians. Bulk rock geochemical data suggest that some metapelitic samples lost a significant amount of melt during interpreted contact metamorphism with the Rich Acres gabbro, resulting in a residual bulk composition (<50 wt% SiO2, ~30 wt% Al2O3). Garnet cores (Grt1) in SiO2‐depleted samples are interpreted to grow during this HT contact metamorphism, with Fe‐Ti oxide thermometry on spinel inclusions in Grt1, cordierite–garnet thermometry, and thermodynamic forward modelling constraining peak P–T conditions during contact heating of the migmatites to ~800ºC and ∼0.5 GPa. This is associated with an inferred peak assemblage prior to melt loss of crd+kfs+pl+grt+bt+spl (mag+usp+hc)+ilm+sil+qtz+melt. Garnet in SiO2‐depleted samples has a distinct high‐Ca rim (Grt2), which appears to record a younger metamorphic event. A combination of substantial melt loss and later rehydration appears to be a major control on the ability of SiO2‐depleted samples to faithfully record evidence for this polymetamorphism. The tectonic implications of this younger metamorphic event are not entirely clear, but it appears to record renewed burial and heating of the SRA sometime after the Taconic orogeny, which may be related to either the neo‐Acadian or Alleghanian orogenies.
Spontaneous meningoencephalitis by Staphylococcus aureus in an infant golden‐headed lion tamarin (Leontopithecus chrysomelas)
Authors: Molina Camila V. , Bueno Marina G. , Kierulff Maria Cecília M. , Pissinatti Alcides , Cunha Marcos P. V. , Knöbl Terezinha , Catão‐Dias José L. , Díaz‐Delgado Josué 
Published: September 2019

AbstractNon‐human primates are susceptible to many bacteria, some of which bear zoonotic potential. We report the pathologic features of spontaneous fulminating meningoencephalitis by Staphylococcus aureus in a captive infant golden‐headed lion tamarin (Leontopithecus chrysomelas) from Brazil.
Medical conditions revealed in fairy tales, folklore and literature
Authors: Massie John 
Published: September 2019

AbstractOur culture has a rich history of fairy tales, folklore and literature. These have all served a purpose, often to entertain, but also to moralise. Authors have often included interesting characters with identifiable medical conditions or described interesting characters that lend their names to conditions we can identify today. This paper looks at the medical conditions found in fairy tales, folklore and literature.
Appendicitis or non‐specific abdominal pain in pre‐school children: When to request abdominal ultrasound?
Authors: Prada‐Arias Marcos , Gómez‐Veiras Javier , Vázquez José L , Salgado‐Barreira Ángel , Montero‐Sánchez Margarita , Fernández‐Lorenzo José R 
Published: September 2019

AimThe aim of this study is to determine the most useful diagnostic tools to differentiate appendicitis from non‐specific abdominal pain (NSAP) in pre‐school children.MethodsWe prospectively evaluated all children aged 5 years or younger admitted for suspected appendicitis at a paediatric emergency department during 5 years. Cases of NSAP and appendicitis were enrolled according to inclusion and exclusion criteria. The different variables collected were assessed by statistic and diagnostic accuracy studies.ResultsA total of 82 patients were studied: 27 cases of NSAP and 55 cases of appendicitis. We found no symptoms or signs with a high power of discrimination between both processes. Complicated cases of appendicitis begin to appear when the duration of symptoms exceeds 12 h. Among laboratory tests, C‐reactive protein (CRP) value >34 mg/L was the variable with a greater association to appendicitis (odds ratio 9.8). Abdominal ultrasound (US) had high sensitivity and specificity to differentiate appendicitis, significantly improving its diagnostic accuracy when the duration of symptoms exceeds 12 h.ConclusionsA good history and physical examination are important in the diagnostic process, but reliable physical signs can be difficult to elicit in pre‐school children. CRP and abdominal US are useful investigations that can improve diagnostic accuracy. According to our results, abdominal pain duration longer than 12 h or CRP value >34 mg/L should be an indication to perform an abdominal US in pre‐school children with right lower quadrant tenderness.
Jersey steer ruminal papillae histology and nutrigenomics with diet changes
Authors: Novak Taylor E. , Rodriguez‐Zas Sandra L. , Southey Bruce R. , Starkey Jessica D. , Stockler Ricardo M. , Alfaro Gastón F. , Moisá Sonia J. 
Published: September 2019

AbstractThe transition from a high forage to a high concentrate diet is an important milestone for beef cattle moving from a stocker system to the feedlot. However, little is known about how this transition affects the rumen epithelial gene expression. This study assessed the effects of the transition from a high forage to a high concentrate diet as well as the transition from a high concentrate to a high forage diet on a variety of genes as well as ruminal papillae morphology in rumen fistulated Jersey steers. Jersey steers (n = 5) were fed either a high forage diet (80% forage and 20% grain) and transitioned to a high concentrate diet (20% forage and 80% grain) or a high concentrate diet (40% forage and 60% grain) and transitioned to a high forage diet (100% forage). Papillae from the rumen were collected for histology and RT‐qPCR analysis. Body weight had a tendency for significant difference (p = .08). Histological analysis did not show changes in papillae length or width in steers transitioning from a high forage to a high concentrate diet or vice versa (p > .05). Genes related to cell membrane structure (CLDN1, CLDN4, DSG1), fatty acid metabolism (CPT1A, ACADSB), glycolysis (PFKL), ketogenesis (HMGCL, HMGCS2, ACAT1), lactate/pyruvate (LDHA), oxidative stress (NQO1), tissue growth (AKT3, EGFR, EREG, IGFBP5, IRS1) and the urea cycle (SLC14A1) were considered in this study. Overall, genes related to fatty acid metabolism (ACADSB) and growth and development (AKT3 and IGFBP5) had a tendency for a treatment × day on trial interaction effect. These profiles may be indicators of rumen epithelial adaptations in response to changes in diet. In conclusion, these results indicate that changes in the composition of the diet can alter the expression of genes with specific functions in rumen epithelial metabolism.
Effect of dietary saponin rich soapnut (Sapindus mukorossi) shell powder on growth performance, immunity, serum biochemistry and gut health of broiler chickens
Authors: Bera Indrajit , Tyagi Praveen K. , Mir Nasir Akbar , Begum Jubeda , Dev Kapil , Tyagi Pramod K. , Biswas Avishek , Sharma Divya , Mandal Asit Baran 
Published: September 2019

AbstractThis study was conducted to evaluate the effects of dietary soapnut (Sapindus mukorossi) shell powder (SSP), a cheap source of saponins, on growth performance, immunity, serum biochemistry and gut health of broiler chickens. The experimental design was 4×2, employing four saponin levels (0, 100, 150 and 200 mg/kg diet), each provided for two time durations (0–42 day and 21–42 day) resulting into eight dietary treatments. Results revealed no significant effect of dietary saponins on body weight gain, feed intake and feed conversion ratio of birds. The abdominal fat percentage, heterophil to lymphocyte ratio, serum cholesterol and triglyceride levels, faecal total plate count, coliform count and E. coli count decreased (p < .05) progressively with increasing saponin levels and lower values were observed at 150 mg and 200 mg saponin levels. Significant improvement of cell‐mediated and humoral immune response was observed in birds fed 150 mg and 200 mg saponin compared to control. The serum glucose concentration was significantly (p < .05) higher in control group compared to other groups. No significant effects of dietary saponin were observed on carcass characteristics, faecal Lactobacillus count, intestinal histomorphometry and cost economics of broiler chicken production. Thus, dietary saponins at 150 mg/kg diet as SSP for three weeks (21–42 days) was optimum for better immunity and welfare of birds without adverse effects on the growth performance.
Does Ethnolinguistic Diversity Preclude Good Governance? A Comparative Study with Alternative Data, 1990‐2015
Authors: de Soysa Indra , Almås Synøve 
Published: September 2019

SUMMARYTo what extent does ethno‐linguistic diversity hinder good governance? Using a variety of data measuring political and economic corruption for 150 countries over 24 years, we find positive effects between ethno‐linguistic diversity and corruption, but the substantive effects are very slight. Higher diversity also predicts higher economic freedom and its subcomponents, measures routinely used as proxies of sound economic governance. Indicators of ethnic frictions measured as ethnic exclusion from state power and discrimination, show positive effects on good governance, results at odds with the idea that governance is harmed by fraught ethnic relations. These findings suggest that the effects of societal diversity do not uniformly explain the failure of governance. We also find diversity to matter negatively when assessing violent state repression of dissent. The results are robust to a host of alternative specifications, data, and estimating strategy, including the inclusion of region fixed effects and hybrid fixed effects.
The ATG16L1 gene variant rs2241880 (p.T300A) is associated with susceptibility to HCC in patients with cirrhosis
Authors: Reuken Philipp A. , Lutz Philipp , Casper Markus , Al‐Herwi Eihab , Stengel Sven , Spengler Ulrich , Stallmach Andreas , Lammert Frank , Nischalke Hans Dieter , Bruns Tony 
Published: September 2019

AbstractBackground and aimsProtein and organelle turnover by autophagy is a key component to maintain cellular homeostasis. Loss of the autophagy protein ATG16L1 is associated with reduced bacterial killing and aberrant interleukin‐1β production, perpetuating inflammation and carcinogenesis. Here we hypothesized that the functional p.T300A gene variant in ATG16L1 is associated with an increased risk for hepatocellular carcinoma (HCC) in cirrhosis.MethodsA case‐control study was performed using a prospective derivation cohort (107 patients with HCC and 101 controls) and an independent validation cohort (124 patients with HCC and 108 controls) of patients with cirrhosis of any aetiology. ATG16L1 p.T300A (rs2241880) and PNPLA3 p.I148M (rs738409) variants were determined by real‐time PCR.ResultsThe G allele of the ATG16L1 p.T300A variant was more frequent in patients with HCC compared to controls without HCC in the derivation cohort (0.62 vs. 0.51, P = .022) and in the validation cohort (0.59 vs. 0.50, P = .045). In combined analysis, the odds ratios (OR) were 1.76 (95% CI: 1.07‐2.88) for G allele positivity and 2.43 (95% CI: 1.37‐4.31) for p.T300A G allele homozygosity. This association was independent from the presence of a PNPLA3 variant, which was also associated with HCC (OR 2.10; 95% CI: 1.20‐3.66), and it remained significant after adjustment for male sex, age and aetiology in multivariate analysis.ConclusionThe common germ‐line ATG16L1 gene variant is a risk factor for HCC in patients with cirrhosis. Personalized strategies employing the genetic risk conferred by ATG16L1 and PNPLA3 may be used for risk‐based surveillance in cirrhosis.
Transcriptomics of monarch butterflies (Danaus plexippus) reveals that toxic host plants alter expression of detoxification genes and down‐regulate a small number of immune genes
Authors: Tan Wen‐Hao , Acevedo Tarik , Harris Erica V. , Alcaide Tiffanie Y. , Walters James R. , Hunter Mark D. , Gerardo Nicole M. , de Roode Jacobus C. 
Published: September 2019

AbstractHerbivorous insects have evolved many mechanisms to overcome plant chemical defences, including detoxification and sequestration. Herbivores may also use toxic plants to reduce parasite infection. Plant toxins could directly interfere with parasites or could enhance endogenous immunity. Alternatively, plant toxins could favour down‐regulation of endogenous immunity by providing an alternative (exogenous) defence against parasitism. However, studies on genomewide transcriptomic responses to plant defences and the interplay between plant toxicity and parasite infection remain rare. Monarch butterflies (Danaus plexippus) are specialist herbivores of milkweeds (Asclepias spp.), which contain toxic cardenolides. Monarchs have adapted to cardenolides through multiple resistance mechanisms and can sequester cardenolides to defend against bird predators. In addition, high‐cardenolide milkweeds confer monarch resistance to a specialist protozoan parasite (Ophryocystis elektroscirrha). We used this system to study the interplay between the effects of plant toxicity and parasite infection on global gene expression. We compared transcriptional profiles between parasite‐infected and uninfected monarch larvae reared on two milkweed species. Our results demonstrate that monarch differentially express several hundred genes when feeding on A. curassavica and A. incarnata, two species that differ substantially in cardenolide concentrations. These differentially expressed genes include genes within multiple families of canonical insect detoxification genes, suggesting that they play a role in monarch toxin resistance and sequestration. Interestingly, we found little transcriptional response to infection. However, parasite growth was reduced in monarchs reared on A. curassavica, and in these monarchs, several immune genes were down‐regulated, consistent with the hypothesis that medicinal plants can reduce reliance on endogenous immunity.
Polygenic selection drives the evolution of convergent transcriptomic landscapes across continents within a Nearctic sister species complex
Authors: Rougeux Clément , Gagnaire Pierre‐Alexandre , Praebel Kim , Seehausen Ole , Bernatchez Louis 
Published: September 2019

AbstractIn contrast to the plethora of studies focusing on the genomic basis of adaptive phenotypic divergence, the role of gene expression during speciation has been much less investigated and consequently less understood. Yet, the convergence of differential gene expression patterns between closely related species‐pairs might reflect the role of natural selection during the process of ecological speciation. Here, we test for intercontinental convergence in differential transcriptional signatures between limnetic and benthic sympatric species‐pairs of Lake Whitefish (Coregonus clupeaformis) and its sister lineage, the European Whitefish (Coregonus lavaretus), using six replicated sympatric species‐pairs (two in North America, two in Norway and two in Switzerland). We characterized both sequence variation in transcribed regions and differential gene expression between sympatric limnetic and benthic species across regions and continents. Our first finding was that differentially expressed genes (DEG) between limnetic and benthic whitefish tend to be enriched in shared polymorphism among sister lineages. We then used both genotypes and covariation in expression in order to infer polygenic selection at the gene level. We identified parallel outliers and DEG involving genes primarily overexpressed in limnetic species relative to the benthic species. Our analysis finally revealed the existence of shared genomic bases underlying parallel differential expression across replicated species‐pairs from both continents, such as a cis‐eQTL affecting the pyruvate kinase expression level involved in glycolysis. Our results are consistent with a long‐standing role of natural selection in maintaining trans‐continental diversity at phenotypic traits involved in ecological speciation between limnetic and benthic whitefishes.