bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lipidomic profiling of subchronic As4S4 exposure identifies inflammatory mediators as sensitive biomarkers in rats
Authors: Zhou Jing , Ma Hongyue , Wu Yuanyuan , Lv Xiang , Wang Jiajia , Liu Shengjin , Li Dandan , Wang Hengbin , Yan Yanqing , Luo Niancui , Li Quan , Xu Huiqin , Zhang Qichun , Yu Li , Guo Hongbo , Kuzmanov Uros , Di Liuqing , Wu Qinan , Duan Jinao 
Published: October 2018

AbstractArsenic sulfide compounds provide nearly all of the world's supply of arsenic. However, the risk of arsenic trisulfide exposure is still not fully investigated. Here, we systemically assessed the toxicology of As4S4 in rats by combining arsenic metabolite detection, routine testing and lipidomic profiling. It was revealed that the oral administration of As4S4 for two months increased the total arsenic content in the liver reaching a saturation level. Further analysis by anion exchange chromatography coupled with inductively coupled plasma mass spectrometry (ICP-MS) technology showed no trace of inorganic arsenic, but there was significant presence of dimethylarsinic acid (DMA), in the livers of rats. This arsenic metabolite was less toxic to rats and did not induce overt liver pathology and functional injury. In contrast, lipidomic profiling provided a comprehensive map of lipids and uncovered a more complex inflammatory response, exhibiting more sensitive change to arsenic exposure. We observed that metabolites of cyclooxygenase, including PGF2α, dhk PGF2α, 15k PGF2α, 8-iso-PGF2a, PGE2, dhk PGE2, PGD2, 15d-PGD2, and PGJ2, were significantly elevated. But mediators from lipoxygenase, cytochrome P450, docosahexaenoic acid, and eicosapentaenoic acid pathways were not markedly affected. In summary, we identified DMA as the predominant arsenic species in the livers of rats, and found cyclooxygenase-derived lipids as the inflammatory mediators before the development of overt liver injury for subchronic As4S4 exposure. These mediators could translate into potential metabolic biomarkers in early arsenic risk assessment and as targets for therapeutic intervention.
Testing the efficiency of water aeration with the use of ATOL-OXY type air applier
Authors: Glowacka Anna , Bogusławski Bartosz 
Published: October 2018

The purpose of this study was to determine the effectiveness of aeration with the use of an applier (a device for aeration of sludge protected by the patent application no P-416711) by determination of characteristics of its operation depending on the stream of water flowing through the device, the height of the outlet over the water table and multiplication factors of measuring water pumped through the applier. On the basis of laboratory tests/measurements, a mathematical model of the increased concentration of oxygen dissolved in water has been developed.
As a result of the research, a significant increase in oxygen concentration has been achieved only after the first water pumping through the applier. The determined mathematical model can be used to calculate predicted increases of oxygen concentration dissolved in water in variants other than those investigated.
Langzeitergebnis nach autologer Membrana-limitans-interna-Lappenplastik bei nicht verschlossenem Makulaloch
Authors: Guber Josef , Vieregge Max , Scholl Hendrik P. N. , Valmaggia Christophe 
Published: October 2018

Zusammenfassung
Ziel Das Ziel dieser Studie ist es, die Operationstechnik sowie die Langzeitergebnisse nach freiem autologem Membrana-limitans-interna-Lappen (ILM-Lappen) für nicht verschlossene Makulalöcher zu evaluieren.
Methoden Die freie ILM-Lappentechnik wurde als sekundäres Verfahren für nicht schließende Makulalöcher nach fehlgeschlagenem initialem Standardverfahren verwendet. Um das anatomische Ergebnis der Operation zu beurteilen, wurden optische Kohärenztomografie-Aufnahmen (SD-OCT-Aufnahmen) postoperativ durchgeführt, während die bestkorrigierte Sehschärfe (BCVA) verwendet wurde, um das funktionelle Ergebnis zu beurteilen. Alle Patienten wurden über einen Zeitraum von 1 Jahr nachkontrolliert.
Ergebnisse Insgesamt wurden 5 Augen eingeschlossen. Bei allen Patienten konnte das Makulaloch mittels ILM-Lappen erfolgreich verschlossen werden. Eine partielle retinale Regeneration, die als Wiederherstellung der äußeren Grenzmembran und der Ellipsoidzone im SD-OCT dargestellt werden konnte, wurde in allen Fällen beobachtet. Funktionell zeigten die meisten Patienten Verbesserungen der BCVA von 1 – 2 Linien.
Fazit Die autologe ILM-Lappentechnik bei persistierenden Makulalöchern scheint ein sicherer und effektiver Ansatz zu sein. Insgesamt zeigen sich gute anatomische und funktionelle Langzeitergebnisse.
Deutsche Fachgesellschaft für Aktivierendtherapeutische Pflege e. V. (DGATP)
Authors: Bartels Friedhilde 
Published: October 2018

Zusammenfassung
Motivation Vielen Patienten, Bewohnern oder Menschen mit Behinderung wird es erst durch die Aktivierend-therapeutische Pflege ermöglicht, therapeutische
Angebote wahrzunehmen und zu erfahren. Eine ausschließlich aktivierende Pflege genügt dafür nicht. Um die Aktivierend-therapeutische Pflege voranzubringen, hat
sich die DGATP gegründet.
MRE in Sachsen – ein Rückblick auf die Jahre 2013 bis 2017
Authors: Schinzel Anja-Susann 
Published: October 2018

Zusammenfassung
Sensibilisierung Die Zahlen von MRSA-Inzidenzen zeigen in den letzten Jahren einen leichten Rückgang und können als erster Erfolg der
Präventionsstrategien gesehen werden. Um diesen Erfolg nicht zu gefährden, bedarf es einer weiteren Sensibilisierung der Fachöffentlichkeit mit Informationen
zum Antibiotikamanagement und zu Hygienemaßnahmen beim Umgang mit Betroffenen.
Korrektur horizontaler Schielwinkel nach Dekompressionsoperation bei Patienten mit endokriner Orbitopathie – Vergleich von Sehnenverlängerung und einfacher Rücklagerung des M. rectus medialis
Authors: Oeverhaus Michael , Fischer Maragrethe , Schlüter Anke , Esser Joachim , Eckstein Anja 
Published: October 2018

Zusammenfassung
Hintergrund Patienten mit endokriner Orbitopathie (EO) können nach knöcherner Dekompressionsoperation sehr große horizontale Schielwinkel entwickeln, vor allem wenn präoperativ eine massive Muskelverdickung vorliegt. Diese großen Schielwinkel können häufig nicht ausreichend mit Rücklagerungen der Mm. recti mediales korrigiert werden. Eine Sehnenverlängerung mit Interponat aus bovinem Perikard (Tutopatch®) ist dann eine Alternative zur gleichzeitigen Resektion des M. rectus lateralis. Wir berichten hier über unsere klinischen Erfahrungen mit Operationen am M. rectus medialis nach knöcherner 3-Wand-Dekompression.
Methoden Patienten mit ein- und beidseitigen Medialis-Rücklagerungen (MR, BMR) (n = 87) und beidseitigen Medialis-Rücklagerungen kombiniert mit Sehnenverlängerung (n = 60) durch Interponat, an einer oder beiden Seite(n) wurden hinsichtlich des operativen Erfolgs (Restschielwinkel ≤ 5° ggf. ausgeglichen mit Prismen, doppelbildfreies Gebrauchsblickfeld – zentrale 20° mit Kopfzwangshaltung ≤ 10°), der Dosis-Wirkungs-Beziehung (DWB; °/mm) und der postoperativen Augenmotilität retrospektiv nach 3 und 12 Monaten analysiert.
Ergebnisse In allen Patientengruppen zeigte sich eine deutliche geringere Dosis-Wirkungs-Beziehung im Vergleich zur MR ohne vorangegangene Dekompression: 1,2 ± 0,4°/mm für die einseitige (MR) und 1,0 ± 0,3°/mm für die beidseitige Medialis-Rücklagerung (BMR); 0,92 ± 0,3°/mm für die einseitige Sehnenverlängerung kombiniert mit einer MR am anderen Auge (BMR + IP) und 0,87 ± 0,3°/mm für die beidseitige Sehnenverlängerung (BMR + BIP). Da bei der präoperativen Planung eine höhere DWB angenommen wurde, war ein großer Teil der Patienten postoperativ unterkorrigiert. Unter Berücksichtigung der tatsächlichen DWB ergeben sich auch bei diesen sehr schwer erkrankten EO-Patienten akzeptable OP-Erfolgsraten: Bei einfacher Rücklagerung um 90% und bei Sehnenverlängerung um 70%.
Schlussfolgerung Nach knöcherner Orbitadekompression muss man Medialis-Rücklagerungen bzw. Rücklagerungen in Kombination mit Sehnenverlängerung höher dosieren als bei Patienten ohne vorangegangene Dekompression. Einfache Rectus-medialis-Rücklagerungen sind deshalb nur bis zu einem Schielwinkel von 15° sinnvoll. Bei größeren Schielwinkeln bis 25° kann man Interponate einsetzen.
Sleep Profile and Self-Reported Neuropsychiatric Symptoms in Vertically HIV-Infected Adolescents on cART
Authors: García-Navarro Cristina , Ruiz-Saez Beatriz , Sainz Talía , Jiménez de Ory Santiago , Rojo Pablo , Mellado Peña María , Prieto Luis , Muñoz-Fernandez Maria , Zamora Berta , Cuéllar-Flores Isabel , Velo Carlos , Martin-Bejarano Manuela , Ramos José , Navarro Gómez María , González-Tomé Maria 
Published: October 2018

Background Sleep quality (SQ) data in human immunodeficiency virus (HIV) pediatric population are scarce. Our main objective was to assess SQ in our cohort and to determine the impact of antiretroviral therapy (ART) on sleep in a cohort of HIV-infected adolescents on cART.
Materials and Methods The SQ was assessed through the Pittsburgh Sleep Quality Index (PSQI). Neuropsychiatric symptoms (NS) were recorded using an auto-administered questionnaire. To determine the antiretroviral (ARV) impact of efavirenz (EFV) on SQ, patients on EFV versus protease inhibitors-based regimens were compared.
Results Forty-six patients were evaluated (median age: 16 years, interquartile range [IQR]: 10.8, 17)). Age at the start of ART: 1.3 years (0.4, 5.2); 23.9% showed acquired immunodeficiency syndrome (AIDS) category. Median CD4 at baseline was 656 (550, 808) cells/mm3; 91.3% had viral load <50 copies/mL. Median time on cART was 11.3 years (7.5, 15.2). Fifty-two percent of the patients were on EFV-based regimen. No differences were found in clinical and immunovirological variables although patients on EFV were older and were exposed for a longer time to ARV. Poor SQ was found in 26.1% of patients. Most frequent complaints were: sleep disturbances (76.1%), sleep latency (63%), and daytime dysfunction (54.3%). Similarly, there were no significant differences in NS between both treatment groups according to patients' reports but were significantly more common in bad sleepers. Patients on EFV-based regimen were more likely to repeat one or more school grades after the adjustment for age at evaluation and nadir CD4 percentage (odds ratio [OR]: 14.421, confidence interval [CI] 95%: 1.207–172.249, p = 0.035).
Conclusions Sleep complaints and NS are common among our cohort of HIV adolescents on long-term cART and interestingly, not only in those who were receiving EFV.
Levetiracetam is Less Effective than Phenobarbital in Treating Seizures Associated with KCNQ2 Variant of Benign Familial Neonatal Convulsions
Authors: Milrod Charles J. , Palmer Jeremy J. , Milrod Lewis M. 
Published: October 2018

AbstractBenign familial neonatal convulsions (BFNC) is an autosomal dominant disorder that presents with generalized tonic–clonic and partial seizures. Levetiracetam is becoming increasingly popular for the treatment of neonatal seizures due to a benign safety profile. Concern has arisen over possible neurotoxicity and apoptosis due to phenobarbital. A male infant with BFNC due to a KCNQ2 mutation responded to phenobarbital but not to levetiracetam. The patient's brother with the same mutation had seizures on day 4 of life; phenobarbital was administered and he became seizure free. This suggests that levetiracetam is a less effective treatment than phenobarbital for BFNC.
Classic Ehlers–Danlos Syndrome in a Son and Father with a Heart Transplant Performed in the Father
Authors: Sen Paushpala , Butler Merlin 
Published: October 2018

AbstractWe report a 13-year-old male patient with severe orthopedic problems including features of a connective tissue disorder and a heterozygous c.305T > A variant found within exon 3 of the autosomal dominant collagen (COL5A1) gene causing the classic Ehlers–Danlos syndrome. This variant has not been reported previously and identified as having an unknown clinical significance but classified as trending damaging per in silico prediction with high conservation among species. Our patient's father had the same gene variant and similar features of stretchable skin, easy bruising, and multiple joint dislocations. The father had unexplained heart failure requiring cardiac transplantation at 43 years of age.
Long-Term Outcome after Successful Lower Extremity Free Flap Salvage
Authors: Bigdeli Amir , Gazyakan Emre , Schmidt Volker , Bauer Christoph , Germann Günter , Radu Christian , Kneser Ulrich , Hirche Christoph 
Published: October 2018

Background Vascular occlusion after free flap surgery has become a rare complication but still poses a major challenge. It necessitates urgent re-exploration, but the logistic challenge to provide sufficient resources for the emergency intervention remains. The aim of this study was to analyze the long-term outcome after successful lower extremity free flap salvage.
Methods A single-center retrospective study including long-term follow-up was approved by the local ethics committee. From January 1999 to December 2010, a total of 581 free flaps were performed for lower extremity reconstruction. Eighty-six flaps required emergency re-exploration, of which 65 could be salvaged. Fifteen salvaged flaps were excluded from the study because of secondary amputation. Of 50 patients, 29 (6 females and 23 males) were eligible for follow-up. The mean follow-up time was 54.5 ± 32.9 months. Health-related quality of life (Short Form 36 [SF-36]) and scar quality (Vancouver Scar Scale [VSS]) were analyzed.
Results The overall flap survival rate was 94.7% and the total loss rate was 5.3%. The re-exploration rate was 14.8% (86 of 581 flaps). The salvage rate was 75.6% (65 of 86 flaps). Twenty-one free flaps were totally lost (24.4%). Partial flap loss occurred in 12 cases (14.0%); 67.5% of the vascular complications occurred during the first 24 hours, 20.9% between 24 and 72 hours, and 11.6% after more than 72 hours. The mean time from the first signs of impaired flap perfusion to re-exploration was 1.3 ± 0.4 hours, and from free tissue transfer to re-exploration was 16.2 ± 1.9 hours. The overall scar appearance was good with an average VSS score of 4.0 points. The average SF-36 physical component score was 54.4 ± 5.4 and the mental component score was 63.1 ± 10.7.
Conclusion Careful monitoring and the opportunity for urgent re-exploration are the key to success for free flaps salvage. Following these principles, an acceptable long-term outcome can be achieved.
Work-Related Musculoskeletal Discomfort and Injury in Microsurgeons
Authors: Howarth Ashley , Hallbeck Susan , Mahabir Raman , Lemaine Valerie , Evans Gregory , Noland Shelley 
Published: October 2018

Background Long surgical procedures with loupe magnification and microscopes may put microsurgeons at an increased risk of musculoskeletal discomfort. Identifying the prevalence and impact of work-related musculoskeletal discomfort may guide preventive strategies to prolong well-being, job satisfaction, and career duration.
Methods An online 29-question survey was designed to evaluate work-related musculoskeletal discomfort. The survey was created and distributed electronically through a private survey research center and was sent to the members of the American Society for Reconstructive Microsurgery.
Results There were 117 respondents (16.7% response rate): 80% were men; 69% were aged 31 to 50 years; and 68% were in academic practice. On a scale of 0 to 10 (0, no pain and 10, worst pain), the median for work-related musculoskeletal discomfort for surgery without loupes or microscope was 2; with loupes, 4; and with a microscope, 5. Pain was most common in the neck. Half of the surgeons reported pain within 4 hours of surgery, and 57% feared that pain would influence future surgical performance. Surgeon discomfort affected posture (72%), stamina (36%), sleep (29%), relationships (25%), concentration (22%), and surgical speed (19%). Tremor caused by the discomfort occurred in 8%. Medical treatment for discomfort was sought by 29%. Time off work for treatment occurred for 8%.
Conclusion Work-related musculoskeletal discomfort can affect many aspects of a microsurgeon's life and has the potential to limit a surgeon's ability to operate. Therefore, more emphasis is needed in the surgical community on the important issues of occupational health and surgical ergonomics for microsurgeons.
Focal adhesion molecules regulate astrocyte morphology and glutamate transporters to suppress seizure-like behavior
Authors: Cho Sukhee , Muthukumar Allie K. , Stork Tobias , Coutinho-Budd Jaeda C. , Freeman Marc R. 
Published: October 2018

Significance
Astrocytes are nonneuronal cells that closely associate with neuronal synapses to regulate synaptic function in brain circuits. A powerful mechanism by which astrocytes can modulate neurotransmission is through their uptake of synaptically released neurotransmitters (NTs). Imbalances in the uptake of the excitatory NT glutamate or inhibitory NT GABBA can change the balance of excitatory and inhibitory signaling in the brain and lead to hyperactivity or seizure. In this study, we explore how astrocytes modulate neuronal hyperactivity and show that focal adhesions, a class of cell-adhesion molecules, are key regulators of astrocyte associations with synapses and astrocyte expression of NT transporters. This work provides potential targets for therapeutic manipulation to suppress seizure activity or excess neuronal excitability.
Shift from androgen to estrogen action causes abdominal muscle fibrosis, atrophy, and inguinal hernia in a transgenic male mouse model
Authors: Zhao Hong , Zhou Ling , Li Lin , Coon V John , Chatterton Robert T. , Brooks David C. , Jiang Enze , Liu Li , Xu Xia , Dong Zhiyong , DeMayo Francesco J. , Stulberg Jonah J. , Tourtellotte Warren G. , Bulun Serdar E. 
Published: October 2018

Significance
Inguinal hernia is one of the most common disorders that affect elderly men. A major pathology underlying inguinal hernia is the fibrosis and other degenerative changes that affect the lower abdominal muscle strength adjacent to the inguinal canal. Here we describe a critical role of estrogen and its nuclear receptor that enhance fibroblast proliferation and muscle atrophy, leading to inguinal hernia. Further research may reveal a potential role of estrogen ablation to prevent muscle fibrosis or hernia in a subset of elderly men.
Categorization of endoplasmic reticulum stress as accumulation of unfolded proteins or membrane lipid aberrancy using yeast Ire1 mutants
Authors: Tran Duc Minh , Takagi Hiroshi , Kimata Yukio 
Published: October 2018

ABSTRACT
Endoplasmic reticulum (ER)-located protein Ire1 triggers the unfolded protein response against ER-stressing stimuli, which are categorized as ER accumulation of unfolded proteins or membrane lipid-related aberrancy. Here we demonstrate that by using yeast Ire1 mutants, we can distinguish the category to which a stress-inducing stimulus belongs. For instance, ethanol was found to activate Ire1 through both types of cellular damage.
Single residue mutation in integrase catalytic core domain affects feline foamy viral DNA integration
Authors: Lee Ga-Eun , Kim Jinsun , Shin Cha-Gyun 
Published: October 2018

ABSTRACTDD(35)E motif in catalytic core domain (CCD) of integrase (IN) is extremely involved in retroviral integration step. Here, nine single residue mutants of feline foamy virus (FFV) IN were generated to study their effects on IN activities and on viral replication. As expected, mutations in the highly conserved D107, D164, and E200 residues abolished all IN catalytic activities (3′-end processing, strand transfer, and disintegration) as well as viral infectivity by blocking viral DNA integration into cellular DNA. However, Q165, Y191, and S195 mutants, which are located closely to DDE motif were observed to have diverse levels of enzymatic activities, compared to those of the wild type IN. Their mutant viruses produced by one-cycle transfection showed different infectivity on their natural host cells. Therefore, it is likely that effects of single residue mutation at DDE motif is critical on viral replication depending on the position of the residues.
Expanded functionality, increased accuracy, and enhanced speed in the de novo genotyping-by-sequencing pipeline GBS-SNP-CROP
Authors: Melo Arthur T O , Hale Iago 
Published: October 2018

Abstract

Summary
GBS-SNP-CROP is a bioinformatics pipeline originally developed to support the cost-effective genome-wide characterization of plant genetic resources through paired-end genotyping-by-sequencing (GBS), particularly in the absence of a reference genome. Since its 2016 release, the pipeline’s functionality has greatly expanded, its computational efficiency has improved, and its applicability to a broad set of genomic studies for both plants and animals has been demonstrated. This note details the suite of improvements to date, as realized in GBS-SNP-CROP v.4.0, with specific attention paid to a new integrated metric that facilitates reliable variant identification despite the complications of homologs. Using the new de novo GBS read simulator GBS-Pacecar, also introduced in this note, results show an improvement in overall pipeline accuracy from 66% (v.1.0) to 84% (v.4.0), with a time saving of ∼70%. Both GBS-SNP-CROP versions significantly outperform TASSEL-UNEAK; and v.4.0 resolves the issue of non-overlapping variant calls observed between UNEAK and v.1.0.

Availability and implementation
GBS-SNP-CROP source code and user manual are available at https://github.com/halelab/GBS-SNP-CROP. The GBS read simulator GBS-Pacecar is available at https://github.com/halelab/GBS-Pacecar.

Supplementary information
Supplementary data are available at Bioinformatics online.
Quality assessment for the putative intrinsic disorder in proteins
Authors: Hu Gang , Wu Zhonghua , Oldfield Christopher J , Wang Chen , Kurgan Lukasz 
Published: October 2018

AbstractMotivationWhile putative intrinsic disorder is widely used, none of the predictors provides quality assessment (QA) scores. QA scores estimate the likelihood that predictions are correct at a residue level and have been applied in other bioinformatics areas. We recently reported that QA scores derived from putative disorder propensities perform relatively poorly for native disordered residues. Here we design and validate a general approach to construct QA predictors for disorder predictions.ResultsThe QUARTER (QUality Assessment for pRotein inTrinsic disordEr pRedictions) toolbox of methods accommodates a diverse set of ten disorder predictors. It builds upon several innovative design elements including use and scaling of selected physicochemical properties of the input sequence, post-processing of disorder propensity scores, and a feature selection that optimizes the predictive models to a specific disorder predictor. We empirically establish that each one of these elements contributes to the overall predictive performance of our tool and that QUARTER’s outputs significantly outperform QA scores derived from the outputs generated the disorder predictors. The best performing QA scores for a single disorder predictor identify 13% of residues that are predicted with 98% precision. QA scores computed by combining results of the ten disorder predictors cover 40% of residues with 95% precision. Case studies are used to show how to interpret the QA scores. QA scores based on the high precision combined predictions are applied to analyze disorder in the human proteome.Availability and implementationhttp://biomine.cs.vcu.edu/servers/QUARTER/Supplementary informationSupplementary data are available at Bioinformatics online.