bluuebunny/crossref_metadata_2025_split huggingface dataset:
Temporal differentiation of bovine airway epithelial cells grown at an air-liquid interface
Authors: Cozens Daniel , Sutherland Erin , Marchesi Francesco , Taylor Geraldine , Berry Catherine C. , Davies Robert L. 
Published: October 2018

AbstractThere is an urgent need to develop improved, physiologically-relevant in vitro models of airway epithelia with which to better understand the pathological processes associated with infection, allergies and toxicological insults of the respiratory tract of both humans and domesticated animals. In the present study, we have characterised the proliferation and differentiation of primary bovine bronchial epithelial cells (BBECs) grown at an air-liquid interface (ALI) at three-day intervals over a period of 42 days from the introduction of the ALI. The differentiated BBEC model was highly representative of the ex vivo epithelium from which the epithelial cells were derived; a columnar, pseudostratified epithelium that was highly reflective of native airway epithelium was formed which comprised ciliated, goblet and basal cells. The hallmark defences of the respiratory tract, namely barrier function and mucociliary clearance, were present, thus demonstrating that the model is an excellent mimic of bovine respiratory epithelium. The epithelium was fully differentiated by day 21 post-ALI and, crucially, remained healthy and stable for a further 21 days. Thus, the differentiated BBEC model has a three-week window which will allow wide-ranging and long-term experiments to be performed in the fields of infection, toxicology or general airway physiology.
The quantum Zeno and anti-Zeno effects with non-selective projective measurements
Authors: Majeed Mehwish , Chaudhry Adam Zaman 
Published: October 2018

AbstractIn studies of the quantum Zeno and anti-Zeno effects, it is usual to consider rapid projective measurements with equal time intervals being performed on the system to check whether or not the system is in the initial state. These projective measurements are selective measurements in the sense that the measurement results are read out and only the case where all the measurement results correspond to the initial state is considered in the analysis of the effect of the measurements. In this paper, we extend such a treatment to consider the effect of repeated non-selective projective measurements – only the final measurement is required to correspond to the initial state, while we do not know the results of the intermediate measurements. We present a general formalism to derive the effective decay rate of the initial quantum state with such nonselective measurements. Importantly, we show that there is a difference between using non-selective projective measurements and the usual approach of considering only selective measurements only if we go beyond the weak system-environment coupling regime in models other than the usual population decay models. As such, we then apply our formalism to investigate the quantum Zeno and anti-Zeno effects for three exactly solvable system-environment models: a single two-level system undergoing dephasing, a single two-level system interacting with an environment of two-level systems and a large spin undergoing dephasing. Our results show that the quantum Zeno and anti-Zeno effects in the presence of non-selective projective measurements can differ very significantly as compared to the repeated selective measurement scenario.
Formation of massive iron deposits linked to explosive volcanic eruptions
Authors: Ovalle J. Tomás , La Cruz Nikita L. , Reich Martin , Barra Fernando , Simon Adam C. , Konecke Brian A. , Rodriguez-Mustafa María A. , Deditius Artur P. , Childress Tristan M. , Morata Diego 
Published: October 2018

AbstractThe genetic link between magmas and ore deposit formation is well documented by studies of fossil hydrothermal systems associated with magmatic intrusions at depth. However, the role of explosive volcanic processes as active agents of mineralization remains unexplored owing to the fact that metals and volatiles are released into the atmosphere during the eruption of arc volcanoes. Here, we draw on observations of the uniquely preserved El Laco iron deposit in the Central Andes to shed new light on the metallogenic role of explosive volcanism that operates on a global scale. The massive magnetite (Fe3O4) ore bodies at El Laco have surface structures remarkably similar to basaltic lava flows, stimulating controversy about their origin. A long-standing debate has endured because all proposed models were constructed based exclusively on samples collected from surface outcrops representing the uppermost and most altered portion of the deposit. We overcome this sampling bias by studying samples retrieved from several drill cores and surface outcrops. Our results reveal complex lithological, textural and geochemical variations characterized by magmatic-like features and, most notably, a systematic increase in titanium concentration of magnetite with depth that account for an evolving system transitioning from purely magmatic to magmatic-hydrothermal conditions. We conclude that El Laco, and similar deposits worldwide, formed by a synergistic combination of common magmatic processes enhanced during the evolution of caldera-related explosive volcanic systems.
Relationship between Ocular Deviation and Visual Function in Retinitis Pigmentosa
Authors: Miyata Manabu , Oishi Akio , Ogino Ken , Oishi Maho , Hasegawa Tomoko , Nagasaku Yuko , Ikeda Hanako Ohashi , Ohtsuki Hiroshi , Tsujikawa Akitaka 
Published: October 2018

AbstractIn retinitis pigmentosa (RP), peripheral visual-field loss starts in early stages, whereas central vision loss occurs in advanced stages. Sensory strabismus gradually occurs in RP. We investigated the relationship between ocular deviation and visual function and explored for sensory strabismus risk factors in 119 consecutive patients with RP at various stages. We assessed ocular deviation at far and near distances, that is the central visual field, using the mean deviation (MD) value and visual acuity (VA), and the residual binocular field area, using Goldmann perimetry (GP), in 33 patients. The horizontal ocular deviation at near distance was >10° in 30% patients and correlated with residual visual function. Although there was no effective cut-off value for central visual function, a cut-off residual GP area of 40 cm2 distinguished patients with a larger from those with a smaller horizontal ocular deviation at far distance (P = 0.04). Our findings suggest that visual function is negatively associated with ocular deviation in patients with RP and that the sensory strabismus risk is relatively high for patients with a binocular visual field <40 cm2. Thus, screening for ocular alignment may be necessary for patients with RP-associated severe vision loss as part of their comprehensive care.
Spatial congruence between multiple stressors in the Mediterranean Sea may reduce its resilience to climate impacts
Authors: Ramírez Francisco , Coll Marta , Navarro Joan , Bustamante Javier , Green Andy J. 
Published: October 2018

AbstractClimate impacts on marine ecosystems may be exacerbated by other, more local stressors interacting synergistically, such as pollution and overexploitation of marine resources. The reduction of these human stressors has been proposed as an achievable way of retaining ecosystems within a “safe operating space” (SOS), where they remain resilient to ongoing climate change. However, the operability of an SOS requires a thorough understanding of the spatial distribution of these climate and human impacts. Using the Mediterranean Sea as a case study, we illustrate the spatial congruence between climate and human stressors impacting this iconic “miniature ocean” synergistically. We use long-term, spatially-explicit information on the distribution of multiple stressors to identify those highly impacted marine areas where human stressors should be prioritized for management if the resilience to climate impacts is to be maintained. Based on our spatial analysis, we exemplify how the management of an essential supporting service (seafood provision) and the conservation of a highly impacted Mediterranean sub-region (the Adriatic Sea) may benefit from the SOS framework.
Genome-wide methylation analysis identifies ELOVL5 as an epigenetic biomarker for the risk of type 2 diabetes mellitus
Authors: Hwang Joo-Yeon , Lee Hyo Jung , Go Min Jin , Jang Han Byul , Choi Nak-Hyun , Bae Jae Bum , Castillo-Fernandez Juan E. , Bell Jordana T. , Spector Tim D. , Lee Hye-Ja , Kim Bong-Jo 
Published: October 2018

AbstractGenome-wide DNA methylation has been implicated in complex human diseases. Here, we identified epigenetic biomarkers for type 2 diabetes (T2D) underlying obesogenic environments. In a blood-based DNA methylation analysis of 11 monozygotic twins (MZTW) discordant for T2D, we discovered genetically independent candidate methylation sites. In a follow-up replication study (17 MZTW pairs) for external validation, we replicated the T2D-association at a novel CpG signal in the ELOVL fatty acid elongase 5 (ELOVL5) gene specific to T2D-discordant MZTW. For concordant DNA methylation signatures in tissues, we further confirmed that a CpG site (cg18681426) was associated with adipogenic differentiation between human preadipocytes and adipocytes isolated from the same biopsy sample. In addition, the ELOVL5 gene was significantly differentially expressed in adipose tissues from unrelated T2D patients and in human pancreatic islets. Our results demonstrate that blood-derived DNA methylation is associated with T2D risk as a proxy for cumulative epigenetic status in human adipose and pancreatic tissues. Moreover, ELOVL5 expression was increased in cellular and mouse models of induced obesity-related diabetes. These findings may provide new insights into epigenetic architecture by uncovering methylation-based biomarkers.
Dual Porosity Protein-based Scaffolds with Enhanced Cell Infiltration and Proliferation
Authors: Rasoulianboroujeni Morteza , Kiaie Nasim , Tabatabaei Fahimeh Sadat , Yadegari Amir , Fahimipour Farahnaz , Khoshroo Kimia , Tayebi Lobat 
Published: October 2018

Abstract3D dual porosity protein-based scaffolds have been developed using the combination of foaming and freeze-drying. The suggested approach leads to the production of large, highly porous scaffolds with negligible shrinkage and deformation compared to the conventional freeze-drying method. Scanning electron microscopy, standard histological processing and mercury intrusion porosimetry confirmed the formation of a dual network in the form of big primary pores (243 ± 14 µm) embracing smaller secondary pores (42 ± 3 µm) opened onto their surface, resembling a vascular network. High interconnectivity of the pores, confirmed by micro-CT, is shown to improve diffusion kinetics and support a relatively uniform distribution of isolated human dental pulp stem cells within the scaffold compared to conventional scaffolds. Dual network scaffolds indicate more than three times as high cell proliferation capability as conventional scaffolds in 14 days.
Bulk band inversion and surface Dirac cones in LaSb and LaBi: Prediction of a new topological heterostructure
Authors: Dey Urmimala , Chakraborty Monodeep , Taraphder A. , Tewari Sumanta 
Published: October 2018

AbstractWe perform ab initio investigations of the bulk and surface band structures of LaSb and LaBi and resolve the existing disagreements about the topological property of LaSb, considering LaBi as a reference. We examine the bulk band structure for band inversion, along with the stability of surface Dirac cones (if any) to time-reversal-preserving perturbations, as a strong diagnostic test for determining the topological character of LaSb, LaBi and LaSb-LaBi multilayer. A detailed ab initio investigation of a multilayer consisting of alternating unit cells of LaSb and LaBi shows the presence of band inversion in the bulk and a massless Dirac cone on the (001) surface, which remains stable under the influence of time-reversal-preserving perturbations, thus confirming the topologically non-trivial nature of the multilayer in which the electronic properties can be tailored as per requirement. A detailed $${{\mathbb{Z}}}_{2}$$

Z

2

invariant calculation is performed to arrive at a holistic conclusion.
Variability of crossing phase in older people with Parkinson’s disease is dependent of obstacle height
Authors: Simieli Lucas , Barbieri Fabio Augusto , Orcioli-Silva Diego , Lirani-Silva Ellen , Beretta Victor Spiandor , Santos Paulo Cezar Rocha dos , Gobbi Lilian Teresa Bucken 
Published: October 2018

AbstractOur aim was investigating variability in spatiotemporal parameters and kinetics of obstacle avoidance steps of different height obstacles in people with Parkinson’s disease (PD) and healthy older people. Twenty-eight participants have included (15 PD – stage disease: 2.1 ± 0.4 pts) and 13 healthy older people (control group). Each subject performed 10 trials of the following tasks: low obstacle, intermediate obstacle and high obstacle. The presentation order was randomized by block for each condition and participant. The spatiotemporal parameters was collected by GAITRite. An optoelectronic system (Optotrak Northern Digital Inc.) with 100 Hz of frequency was used to collect obstacle parameters. The kinect parameters (propulsion impulse and braking impulse) were acquire through two force plates (AccuGait), with a frequency of acquisition 200 Hz. Intersteps variability was calculated throughout mean values, standard deviation and coefficient of variation of two obstacle avoidance steps for each trial. PD group presented greater variability than control group on vertical and horizontal distances to the obstacle. Obstacle height did not change kinect’s parameters for both groups. The combination of task complexity (obstacle height) and disease impairments (gait alteration, loss of balance, etc) contributing for greater variability of Parkinson’s group. Besides, low obstacle and high obstacle seem to exacerbate variability of distance between obstacle and foot.
Assembling the genome of the African wild rice Oryza longistaminata by exploiting synteny in closely related Oryza species
Authors: Reuscher Stefan , Furuta Tomoyuki , Bessho-Uehara Kanako , Cosi Michele , Jena Kshirod K. , Toyoda Atsushi , Fujiyama Asao , Kurata Nori , Ashikari Motoyuki 
Published: October 2018

AbstractThe African wild rice species Oryza longistaminata has several beneficial traits compared to cultivated rice species, such as resistance to biotic stresses, clonal propagation via rhizomes, and increased biomass production. To facilitate breeding efforts and functional genomics studies, we de-novo assembled a high-quality, haploid-phased genome. Here, we present our assembly, with a total length of 351 Mb, of which 92.2% was anchored onto 12 chromosomes. We detected 34,389 genes and 38.1% of the genome consisted of repetitive content. We validated our assembly by a comparative linkage analysis and by examining well-characterized gene families. This genome assembly will be a useful resource to exploit beneficial alleles found in O. longistaminata. Our results also show that it is possible to generate a high-quality, functionally complete rice genome assembly from moderate SMRT read coverage by exploiting synteny in a closely related Oryza species.
523676 (2013 UL10): the first active red centaur
Authors: Mazzotta Epifani E. , Dotto E. , Ieva S. , Perna D. , Palumbo P. , Micheli M. , Perozzi E. 
Published: October 2018

Aims. We present observations of 523676 (2013 UL10), a centaur orbiting between Jupiter and Uranus that is dynamically similar to the few tens of active centaurs that are currently known.
Methods. We analysed visible BVR images of the centaur obtained at the Telescopio Nazionale Galileo (La Palma, Canary Islands, Spain) to investigate the weak comet-like activity and to derive information on the nucleus surface colours and size.
Results. Centaur 523676 (2013 UL10) is the only centaur known so far that has both comet-like activity and red surface colours: its nucleus has a colour index [B – R] = 1.88 ± 0.11. The nucleus R magnitude (R = 20.93 ± 0.09) allowed us to derive an upper limit for its nucleus size of D ≤ 10 km. We estimated its dust production rate to be Qd ~ 10 kg s−1 at 6.2 au (just after its perihelion passage), resulting in a timescale for the surface blanketing process τB of approximately tens of years, which is very short with respect to typical dynamical lifetime inside the group. Future monitoring of 523676 (2013 UL10) is needed to further constrain the blanketing model for active centaurs and its timescale.
The 3PAs: An Update on the Association of Pheochromocytomas, Paragangliomas, and Pituitary Tumors
Authors: Xekouki Paraskevi , Brennand Ana , Whitelaw Ben , Pacak Karel , Stratakis Constantine A. 
Published: October 2018

AbstractPituitary adenomas (PA) and pheochromocytomas/paragangliomas (PHEO/PGL) are rare tumors. Although they may co-exist by coincidence, there is mounting evidence that genes predisposing in PHEO/PGL development, may play a role in pituitary tumorigenesis. In 2012, we described a GH-secreting PA caused by an SDHD mutation in a patient with familial PGLs and found loss of heterozygosity at the SDHD locus in the pituitary tumor, along with increased hypoxia-inducible factor 1α (HIF-1α) levels. Additional patients with PAs and SDHx defects have since been reported. Overall, prevalence of SDHx mutations in PA is very rare (0.3–1.8% in unselected cases) but we and others have identified several cases of PAs with PHEOs/PGLs, like our original report, a condition which we termed the 3 P association (3PAs). Interestingly, when 3PAs is found in the sporadic setting, no SDHx defects were identified, whereas in familial PGLs, SDHx mutations were identified in 62.5–75% of the reported cases. Hence, pituitary surveillance is recommended among patients with SDHx defects. It is possible that the SDHx germline mutation-negative 3PAs cases may be due to another gene, epigenetic changes, mutations in modifier genes, mosaicism, somatic mutations, pituitary hyperplasia due to ectopic hypothalamic hormone secretion or a coincidence. PA in 3PAs are mainly macroadenomas, more aggressive, more resistant to somatostatin analogues, and often require surgery. Using the Sdhb

+/−
mouse model, we showed that hyperplasia may be the first abnormality in tumorigenesis as initial response to pseudohypoxia. We also propose surveillance and follow-up approach of patients presenting with this association.
Noninvasive Liver Fibrosis Tests in Patients with Nonalcoholic Fatty Liver Disease: An External Validation Cohort
Authors: Polyzos Stergios , Slavakis Aristidis , Koumerkeridis Georgios , Katsinelos Panagiotis , Kountouras Jannis 
Published: October 2018

AbstractThe main aim of this study was the comparative evaluation of nonalcoholic fatty liver disease (NAFLD) fibrosis score (NFS), fibrosis 4 index (FIB-4), AST-to-Platelet Ratio Index (APRI), and enhanced liver fibrosis (ELF) test in distinguishing none/early (F0/F1) from significant/advanced (F2/F3) fibrosis in NAFLD patients, thereby providing an external validation cohort. Thirty-one patients with biopsy-proven NAFLD and 10 matched controls without NAFLD were prospectively enrolled. Serum hyaluronic acid (HA), aminoterminal propeptide of type III procollagen (PIIINP), tissue inhibitor of metallo-proteinases (TIMP)-1, and biochemical tests were measured. NFS, FIB-4, APRI, and ELF were calculated. ELF, FIB-4, and APRI, but not NFS, were higher in F2/F3 than F0/F1 group. Specifically, ELF [area under the ROC curve (AUROC): 0.86±0.10; p=0.004) and APRI (AUROC: 0.86±0.07; p=0.005], but not NFS (AUROC: 0.68±0.12; p=0.16), and FIB-4 (AUROC: 0.71±0.11; p=0.10), could similarly discriminate F0/F1 from F2/F3 stage. The sensitivity, specificity, positive predicted value (PPV), and negative predicted value (NPV) were: a) for cut-off of APRI=0.5, 85.7%, 70.8%, 46.2%, and 94.4%, respectively, and b) for cut-off of ELF=9.0, 85.7%, 83.3%, 60.0%, and 95.2%, respectively. When ln(PIIINP) or TIMP-1 were combined with APRI, the combined AUROCs could distinguish F2/F3 from F0/F1, but without significantly higher accuracy compared with APRI alone. APRI could also distinguish patients with simple steatosis from nonalcoholic steatohepatitis, and those with from those without lobular inflammation and ballooning, findings warranting further research. In conclusions: The application of ELF test and APRI can distinguish F0/F1 from F2/F3 fibrosis stages in NAFLD patients.
New Era in Denture Base Resins: A Review
Authors: Nandal Shikha , Ghalaut Pankaj , Shekhawat Himanshu , Gulati Manmeet 
Published: October 2018

AbstractIn past, the materials used for denture bases were vulcanite, celluloid & phenol formaldehyde. Acrylic resins were introduced to dentistry in 1930's. There are certain limitations of acrylics like residual monomer allergy, poor mechanical strength, low fatigue strength, brittle on impact, poor conductors of heat, low hardness, high coefficient of thermal expansion, thermal shrinkage, poor color stability of self-cured resins, porosity, crazing, warpage, poor adhesion to metal and porcelain and requirement of mechanical retention. But still they are the most widely used denture base materials till date. But recently there has been much advancement in the field of denture base resins to overcome their drawbacks and to obtain an ideal denture base material. This article reviews the various advancements in the field of denture base resins.
How to Manage Xerostomia in Prosthodontics???
Authors: Singh Opinder , Mittal Neeraj , Saini Rishi ,   Rubina,   Nikita
Published: October 2018

AbstractSaliva is known as the mirror of the body. It is not only essential for retention of removable prosthesis but also protects the oral mucosa from injury by its lubricating effect. Xerostomia or hyposalivation is a commonly encountered condition especially among the elderly that needs to be treated to improve quality of life in denture wearers. This article reviews the various etiologic factors and treatment modalities in patients with xerostomia.
Mineral Trioxide Aggregate in the Treatment of External Invasive Resorption: A Case Report
Authors: Ahir Bhavesh , Parekh Vaishali , Vats Anjali , Vats Ankur 
Published: October 2018

AbstractExternal invasive root resorption may occur as a consequence of trauma, orthodontic treatment, intracoronal bleaching and surgical procedures and may lead to the progressive and destructive loss of tooth structure. Depending on the extent of the resorptive process, different treatment regimens have been proposed. A 20-year-old female patient presented with tooth showing signs and symptoms of pain in 21 with extensive invasive resorption in middle third of root canal. After root canal treatment, The resorption area was chemomechanically debrided using ultrasonic tips and irrigant solution. MTA was used to fill the resorptive defect and the coronal access was temporarily sealed. Radiographs at 3 and 12 months showed adequate repair of the resorption and endodontic success. Clinically, the tooth was asymptomatic and found to be functioning well.
Hemisection for the Treatment of Advanced Endodontic Periodontal Lesion
Authors: Gupta Ashu , Thakur Saroj , Thakur Rambhika 
Published: October 2018

AbstractThe removal of a root and the overlaying anatomic crown is referred to as a hemisection. A case with a complain of pain and bone loss till the apex of distal root of 36 presented in the department of Endodontics, Government dental college, Shimla. The patient had increased desire to maintain the natural dentition. The decision was taken to hemi-sect the distal root, as mesial bone and furcation bone was relatively unaffected. After phase I therapy, periodontal flap was reflected, area debrided and distal root was resected. This article describes a procedure of hemisection in mandibular molar and its subsequent restoration. The key to long term success appear to be thorough diagnosis, selection of patients with good oral hygiene and careful surgical and restorative management.