bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evodiamine alleviates kidney ischemia reperfusion injury in rats: A biochemical and histopathological study
Authors: Eraslan Ersen , Tanyeli Ayhan , Polat Elif , Yetim Zeliha 
Published: May 2019

AbstractRenal ischemia/reperfusion (I/R) injury resulting in acute renal failure, is a major clinical problem due to its high mortality rate. Renal I/R increases the reactive oxygen species, secretion of inflammatory cytokines, chemokines and other factors. This suggests that initiating the apoptosis process in the presence of oxidative stress may play a role in life‐threatening conditions, such as ischemia. Ischemia reperfusion‐induced renal damage can result in renal failure and death. Although many treatment procedures have been carried out to reduce or destroy renal I/R damage in experimental models, so far, a routine method of treatment has not yet been found. For this reason, the current study was planned to investigate the possible protective effects of evodiamine on tissue damage caused by ischemia‐reperfusion in kidney tissue in rats and an experimental renal I/R model was used for this purpose. Four groups were formed in the study: the control, sham control, ischemia reperfusion (I/R), and evodiamine (10 mg/kg) + I/R groups. The effects of evodiamine against kidney I/R injury were investigated. TAS (total oxidant status), TOS (total oxidant status), interleukin‐1β (IL‐1β), IL‐6, IL‐10 and tumor necrosis factor‐α levels were determined by enzyme‐linked immunosorbent assay. The oxidative stress index was calculated from TAS and TOS levels. In addition, the renal ischemia reperfusion injury was examined histopathologically. The IL‐10 and TAS levels in the I/R group decreased when compared with the control and Sham groups, while these levels increased in the evodiamine group. Histopathologic examination revealed that caspase 3 and nuclear factor‐κB levels decreased in the evodiamine group compared with the I/R group. The application of evodiamine significantly reduced ischemia reperfusion‐induced kidney damage due to its antioxidant, anti‐inflammatory and antiapoptotic properties.
Altered expression of microRNA‐365 is related to the occurrence and development of non‐small‐cell lung cancer by inhibiting TRIM25 expression
Authors: Han Qian , Cheng Peng , Yang Hongjie , Liang Hengpo , Lin Fengchun 
Published: May 2019

AbstractThe purpose of this current study is to elucidate whether altered microRNA‐365 (miR‐365) has an association with the initiation and development of non‐small‐cell lung cancer (NSCLC) by targeting TRIM25 expression. The expression of miR‐365 and TRIM25 in NSCLC tissues, adjacent normal tissues, and NSCLC cell lines were detected. The relationship between miR‐365 expression and TRIM25 with the clinicopathological characteristics of NSCLC was analyzed. The putative binding site between miR‐365 and TRIM25 was determined by luciferase activity assay. miR‐365 inhibitors and miR‐365 mimics were transfected to human NSCLC A549 cells, and the cell viability was detected by cell counting kit‐8 assay; flow cytometry was carried out to determine cell cycle and apoptosis rate. Poorly expressed miR‐365 and overexpressed TRIM25 was found in NSCLC tissues. TRIM25 was determined as a target gene of miR‐365. The miR‐365 and TRIM25 expression were related to the clinicopathological features of NSCLC, such as pathological classification, differentiation degree, TNM stage as well as lymph node metastasis. miR‐365 suppressed the expression of TRIM25 and elevated the expression of the proapoptotic protein in NSCLC cells. Our study demonstrates that altered expression of miR‐365 has a close association with the occurrence and development of NSCLC by inhibiting TRIM25 expression.
The small molecule SI113 hinders epithelial‐to‐mesenchymal transition and subverts cytoskeletal organization in human cancer cells
Authors: Abbruzzese Claudia , Matteoni Silvia , Persico Michele , Ascione Barbara , Schenone Silvia , Musumeci Francesca , Amato Rosario , Perrotti Nicola , Matarrese Paola , Paggi Marco G. 
Published: May 2019

AbstractThe small molecule SI113 is an inhibitor of the kinase activity of SGK1, a key biological regulator acting on the PI3K/mTOR signal transduction pathway. Several studies demonstrate that this compound is able to strongly restrain cancer growth in vitro and in vivo, alone or in associative antineoplastic treatments, being able to elicit an autophagic response, either cytotoxic or cytoprotective. To elucidate more exhaustively the molecular mechanisms targeted by SI113, we performed activity‐based protein profiling (ABPP) proteomic analysis using a kinase enrichment procedure. This technique allowed the identification via mass spectrometry of novel targets of this compound, most of them involved in functions concerning cell motility and cytoskeletal architecture. Using a glioblastoma multiforme, hepatocarcinoma and colorectal carcinoma cell line, we recognized an inhibitory effect of SI113 on cell migration, invading, and epithelial‐to‐mesenchymal transition. In addition, these cancer cells, when exposed to this compound, showed a remarkable subversion of the cytoskeletal architecture characterized by F‐actin destabilization, phospho‐FAK delocalization, and tubulin depolimerization. These results were definitely concordant in attributing to SI113 a key role in hindering cancer cell malignancy and, due to its negligible in vivo toxicity, can sustain performing a Phase I clinical trial to employ this drug in associative cancer therapy.
Membrane‐Spanning Protein Genetic Polymorphisms Related to Methotrexate Therapeutic Outcomes in a Chinese Rheumatoid Arthritis Population
Authors: Lv Shuang , Fan Huizhen , Yang Hui , Huang Jing , Li Jiang , Shu Xiaoming , Zhang Lu , Xu Yuan , Li Xiaoya , Zuo Jieyu , Lv Cheng , Kong Xiaomu , Xiao Cheng 
Published: May 2019

AbstractMethotrexate (MTX) is a first‐line disease‐modifying antirheumatic drug for rheumatoid arthritis (RA), but individual variation in treatment response remains unexplained. The differences in drug efficacy and adverse drug reactions may be caused by genetic variations. We investigated the effects of single‐nucleotide polymorphisms (SNPs) in 2 genes encoding membrane‐spanning proteins, namely, reduced folate carrier‐1 RFC‐1/SLC19A1 (G>A [rs7499], A>G [rs2838956] and 180G>A [rs1051266]) and adenosine triphosphate–binding cassette B1 (rs1045642). Tagged SNPs were genotyped in 162 patients with RA in China. Then, we analyzed the relationships between these SNPs and therapeutic outcomes related to MTX in Chinese RA patients. No significant associations were found between the RFC‐1/SLC19A1 (G>A [rs7499] and A>G [rs2838956]) and adenosine triphosphate–binding cassette B1 (rs1045642) gene polymorphisms and the response to MTX in RA patients. However, MTX‐related toxicity was associated with one SNP, RFC‐1 rs1051266 AA vs GG (odds ratio, 6.523; 95% confidence interval, 1.596–26.565;P= .009). SLC19A1 A>G rs2838956 showed a trend toward a significant association (odds ratio, 0.377; 95% confidence interval, 0.124–1.143;P= .085) with toxicity. Our results suggest that the RFC‐1 80G>A (rs1051266) SNP exerts a potentially protective effect against the risk of adverse drug reactions in Chinese RA patients treated with MTX. Further studies are required to validate these findings.
Identification of effective membrane efflux transporters against β‐amyrin through molecular docking approach
Authors: Ahmed Muhammad Saad , Sehgal Sheikh Arslan , Tahir Rana Adnan , Li Chun 
Published: May 2019

AbstractBACKGROUNDMetabolic engineering techniques in microorganisms can produce high‐value complex metabolites. However, this efficiency was frequently hampered by toxic‐compounds produced due to the engineering of foreign genes. Comparatively, to other strategies, the efflux transporter engineering strategy is efficient to create a pulling effect at the downstream of the biosynthetic pathway, generally enhancing the cultural vitality and the total production yields due to which wild strains can withstand toxic compounds. However, it was difficult to select hundreds of transporters for the possible efflux of β‐amyrin. Thus, in silico approaches were used to explore the binding sites of the transporters and identify the stable binding patterns of transporters for the possible efflux of β‐amyrin.RESULTSA total 111 transporters were shortlisted having the ability to transport different compounds. Molecular docking analyses elucidated that YajR, UraA, GlcPse, and LeuT, transporters have strong binding affinities against β‐amyrin having least binding energies of −13.6, −13.4, −12.2, and − 11.9 kcal/mol, respectively.CONCLUSIONFor the first time, it was observed that the mentioned top‐ranked transporters might be the better option to efflux β‐amyrin for being effectively bound within the enclosed active site. These novel findings based on computational docking analyses may be momentous to enhance the metabolites production in the microorganism. © 2019 Society of Chemical Industry
The role of the right atrium in development of postoperative atrial fibrillation: A speckle tracking echocardiography study
Authors: Aksu Uğur , Kalkan Kamuran , Gulcu Oktay , Aksakal Emrah , Öztürk Mustafa , Topcu Selim 
Published: May 2019

AbstractPurposeAtrial fibrillation (AF) is relatively frequent in the postoperative period, and is associated with an increased frequency of adverse events. The role of right atrial (RA) volume and functions in the development of AF is unknown. In this study, we investigated the effect of RA echocardiographic indices on AF development in the postoperative period.MethodWe enrolled 142 consecutive patients who underwent coronary artery bypass surgery, and assigned them into two groups depending on the occurrence or not of AF development in the postoperative period.ResultsA propensity score matching analysis was performed to balance the groups, and 37 pairs were eventually included in the analysis. The median age was 67.5 (63‐75) years and 73.3% of them were males. In the univariate analysis, right atrial volume index (RAVi), right atrial strain during reservoir phase (RASr), left ventricular global longitudinal strain, right ventricular strain, left atrial volume index, left atrial strain during reservoir phase, and systolic pulmonary artery pressure were associated with AF development. In the regression analysis, we found that RAVi (OR: 3.1, 95% CI: 2.2‐6.3, P: .033) and RASr (OR: 0.82, 95% CI: 0.67‐0.93, P: .048) were independent predictors of AF development.ConclusionsRA structure and functions are closely associated with AF development in the postoperative period, and screening of RA functions prior to surgery may be useful for preventing AF development.
The role of spatial frequency analysis in correlating atomic force microscopy and optical profilometry with self‐etch adhesive enamel bond fatigue durability
Authors: Fischer Nicholas G. , Dang Jonathan , Takamizawa Toshiki , Tsujimoto Akimasa , Barkmeier Wayne W. , Baruth Andrew G. 
Published: May 2019

AbstractThe purpose of this study was to: (a) evaluate the role of enamel surface roughness on bond fatigue durability and (b) evaluate statistical differences in roughness values based on measurement technique, including the use of spatial filtering for optical profilometry (OP). OptiBond XTR (Kerr Corp), Prime & Bond elect (DENTSPLY Caulk), Scotchbond Universal (3 M Oral Care), and XTR pre‐etched with Ultra‐Etch phosphoric acid (35%) (Ultradent) self‐etch adhesives were used to treat enamel. A flat ground enamel surface was included as a control. Atomic force microscopy (AFM) and OP were used to measure the surface topography of each enamel surface following the application of adhesives. AFM, OP, and filtered OP (FOP) roughness values, where FOP was designed to only include the lateral spatial resolution consistent with AFM roughness values, were collected. Spatial resolution filtering with OriginPro was used to compare line scans from the two imaging techniques and generate the FOP group. These micro‐ versus nanoscale lateral roughness values were correlated with shear bond and shear fatigue strengths of the adhesives bonded to enamel. Roughness values showed differences based on measurement technique and strong correlations with bond and fatigue strength. The filtered OP group demonstrated the importance of careful usage and reporting of atomic force microscopy and OP metrics in adhesive dentistry. Best practices for surface roughness analysis were also discussed.
Gastroduodenal artery: single key for many locks
Authors: Desai Gunjan S. , Pande Prasad M. 
Published: May 2019

AbstractGastroduodenal artery (GDA) commonly arises from common hepatic artery, a branch of celiac axis. It holds a unique anatomical position that connects the foregut and midgut due to its intimate communications with foregut and midgut arterial supply. Its numerous anatomical variations have a significant impact on planning and performance of hepatopancreaticobiliary (HPB) surgery. Its close relation to the first part of duodenum, common bile duct and head of pancreas makes it susceptible for inadvertent bleeding during or after surgery, or due to various HPB pathologies. Also, a large number of vascular interventions rely on GDA and its branches. Careful preoperative planning is the key and a detailed knowledge and awareness of its variant anatomy is of paramount importance, be it liver resections, liver transplant, biliary and pancreatic resections and pancreatic transplant or transarterial procedures involving these arteries. GDA can also be a cause of gastrointestinal hemorrhage due to true or pseudoaneurysms and anatomy has significant implications on its management. The article provides a succinct review on relevance of GDA anatomy and variations and highlights that preoperative planning and intraoperative awareness of variations is the key to performance of safe HPB surgery and interventions.
Complete genome sequence analysis of temperate Erwinia bacteriophages 49 and 59
Authors: Zlatohurska Maryna , Gorb Tetiana , Romaniuk Liudmyla , Korol Natalia , Faidiuk Yuliia , Kropinski Andrew M. , Kushkina Alla , Tovkach Fedor 
Published: May 2019

AbstractTo date, a small number of temperate phages are known to infect members of the genus Erwinia. In this study, the genomes of temperate phages vB_EhrS_49 and vB_EhrS_59 infecting Erwinia horticola, the causative agent of beech black bacteriosis in Ukraine, were sequenced and annotated. Their genomes reveal no significant similarity to that of any previously reported viruses of Enterobacteriaceae. At the same time, phages 49 and 59 share extensive nucleotide sequence identity across the regions encoding head assembly, DNA packaging, and lysis. Despite significant homology between structural modules, the organization of distal tail morphogenesis genes is different. Furthermore, a number of putative morons and DNA methylases have been found in both phage genomes. Due to the revealed synteny as well as the structure of lysogeny module, phages 49 and 59 are suggested to be novel members of the lambdoid phage group. Conservative structural genes together with varying homology across the nonstructural region of the genomes make phages 49 and 59 highly promising objects for studying the genetic recombination and evolution of microbial viruses. The obtained data may as well be helpful for better understanding of relationships among Erwinia species.
An assessment of the impact of Pacific Decadal Oscillation on autumn droughts in North China based on the Palmer drought severity index
Authors: Wang Min , Gu Qinxue , Jia Xiaojing , Ge Jingwen 
Published: May 2019

AbstractThis study assesses the impact of the Pacific Decadal Oscillation (PDO) on the severity of autumn droughts in North China (ADNC) during the period of 1962–2013. The ADNC are defined using the Palmer drought severity index (PDSI). The results show that the ADNC are more severe during negative PDO phases than during positive PDO phases. During the negative PDO phase, both the autumn precipitation and the Penman–Monteith reference evaporation (PET_pm) play roles in the significant severity of the ADNC. In contrast, during positive PDO phases, the droughts over North China are impacted to a greater degree by the climate conditions in previous seasons. Regression analysis shows that the atmospheric circulation anomalies associated with ADNC are quite different over North China under different PDO phases. Specifically, during negative PDO phases, anomalous low‐pressure systems are observed over the East Asia‐western Japan area, indicating a strengthened East Asian trough (EAT). Anomalous northerly winds bring cold and dry air from high‐latitude inland regions to North China, leading to severe ADNC during this period. Meanwhile, significant descending motion is observed in the upstream region of the EAT and the area of North China, accompanied by reduced cloudiness and reduced precipitation over these regions. In contrast, during positive PDO phases, the ADNC‐associated atmospheric circulation anomalies over North China are quite weak. Further analysis shows that during the negative phase of PDO years, the El Niño Southern Oscillation can impact the severity of ADNC through the centre of the Pacific‐East Asian teleconnection pattern over northeastern Asia.
Antarctic sea‐ice variation associated with vertical geopotential height and temperature anomalies
Authors: Qian Weihong , Wu Kaijun , Leung Jeremy Cheuk‐Hin 
Published: May 2019

AbstractIt is well‐known that the increasing zonal‐mean trend of sea‐ice concentration (SIC) surrounding Antarctica is up to 2015 so this paper first summarized this trend with opposing regional trends in each season along the latitude band 60°–70°S during 1979–2015. The surface wind trends can partly explain the observed regional SIC trends. By analysing the surface wind, as well as the tropospheric geopotential height (GPH) and temperature trends, the study revealed that the increasing zonal‐mean SIC trend and opposing regional SIC trends surrounding Antarctica are vertically associated with GPH and temperature trends in the troposphere. This relationship is also found in extreme years for seven SIC maximum and four SIC minimum autumns from 1979 to 2015, particularly reconfirmed in the two SIC minimum autumns of 2017 and 2018, indicating that a positive regional SIC anomaly is vertically associated with an anomalously cold air mass centred around 850 hPa in the troposphere which is located beneath a negative GPH anomaly at the upper troposphere, and vice versa.
Influence of ambient temperature on OLED lifetime and uniformity based on modified equivalent lifetime detection
Authors: Fan Rui , Zhang Xiaoning , Tu Zhentao 
Published: May 2019

AbstractThe ambient temperature can affect the OLED heat dissipation and aggravate the luminance degradation. Based on temperature‐related equivalent lifetime detection, a luminance compensation algorithm without internal or external sensing circuits is proposed and the influence of ambient temperature on lifetime and display uniformity is studied. First, the parameters of luminance degradation curves at any ambient temperature are calculated according to the relationship between accelerate degradation factor n and ambient temperature T, which is determined by the measured luminance degradation curves at ambient temperature 25°C, 45°C, and 80°C. Second, luminance compensation is carried out by increasing drive current according to the estimated luminance degradation influenced by temperature. Furthermore, compensation iterations m, compensation goal value Lgoal, and compensation origin value Lorigin are optimized to obtain a better lifetime extension at different ambient temperature. The simulated and experimental results indicate that the lifetime extensions considering the influence of ambient temperature at 0°C, 25°C, 45°C, 60°C, and 80°C are 29.7%, 28.1%, 18.7%, 18.9%, and 11.6%. Meanwhile, after displaying an image of the International Electrotechnical Commission (IEC) 62087 for 90 hours, the luminance uniformity of OLED increase to 96.0%, 94.0%, 97.2%, 97.2%, and 98.6%, which is a significant improvement compared with it of regardless the influence of the ambient temperature.
Dually Cross‐Linked Supramolecular Hydrogel as Surface Plasmon Resonance Sensor for Small Molecule Detection
Authors: Li Jie , Ji Chendong , Yu Xiaoqian , Yin Meizhen , Kuckling Dirk 
Published: May 2019

AbstractDually cross‐linked supramolecular hydrogels (DCSH) are designed to show responsive properties while maintaining the gel structure by introducing two different kinds of cross‐links. This is realized by utilizing a photo‐cross‐linker for permanent cross‐linking and β‐cyclodextrin (β‐CD) and ferrocene (Fc) as host–guest recognition pair. The DCSH shows increased swelling in the presence of the small target molecule adamantane amine (Ada). Ada can break the non‐covalent bonding between β‐CD and Fc through competitive molecular guest interaction with β‐CD. By using a combination of surface plasmon resonance and optical waveguide spectroscopy, it is possible to use this behavior to construct a reversible sensor for specific small molecule detection.
Looking for alternative treatments for bovine and caprine mastitis: Evaluation of the potential of Calliandra surinamensis leaf pinnulae lectin (CasuL), both alone and in combination with antibiotics
Authors: Procópio Thamara F. , Moura Maiara C. , Bento Elinaldo F. L. , Soares Tatiana , Coelho Luana C. B. B. , Bezerra Raquel P. , Mota Rinaldo A. , Porto Ana Lúcia F. , Paiva Patrícia M. G. , Napoleão Thiago H. 
Published: May 2019

AbstractThis work aimed to evaluate the effects of CasuL on growth and viability of 15 mastitis isolates from cows and goats, to determine the synergistic potential between CasuL and antibiotics, and to investigate the effects on bacterial ultrastructure and antibiofilm activity. The lectin inhibited the growth of Staphylococcus isolates from either bovine (Ssp6PD and Sa) or caprine (Ssp5D and Ssp01) mastitis. The minimal inhibitory concentrations were ranged from 3.75 to 15 µg/ml. Synergistic effect was observed for CasuL‐tetracycline against Sa and Ssp6PD and CasuL‐ampicillin against Ssp01. No structural damage was observed under the scanning electron microscope in CasuL treatments. Flow cytometry analysis using thiazol orange and propidium iodide demonstrated that CasuL was unable to reduce the cell viability of the isolates tested. At sub‐inhibitory concentrations, CasuL reduced biofilm formation by the isolates Sa and Ssp5D. However, CasuL‐tetracycline and CasuL‐ampicillin combinations inhibited biofilm formation by Ssp6PD and Ssp01, respectively. In conclusion, CasuL is a bacteriostatic and antibiofilm agent against some mastitis isolates and displayed a synergistic potential when used in combination with either ampicillin (against one isolate) or tetracycline (against two isolates). The results stimulate the evaluation of CasuL for the treatment of mastitis, particularly when used in conjunction with antibiotics.