bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome-scale metabolic reconstruction and metabolic versatility of an obligate methanotrophMethylococcus capsulatusstr. Bath
Authors: Gupta Ankit , Ahmad Ahmad , Chothwe Dipesh , Madhu Midhun K. , Srivastava Shireesh , Sharma Vineet K. 
Published: June 2018

AbstractThe increase in greenhouse gases with high global warming potential such as methane is a matter of concern and requires multifaceted efforts to reduce its emission and increase its mitigation from the environment. Microbes such as methanotrophs can assist in methane mitigation. To understand the metabolic capabilities of methanotrophs, a complete genome-scale metabolic model of an obligate methanotroph,Methylococcus capsulatusstr. Bath was reconstructed. The model contains 535 genes, 898 reactions and 784 unique metabolites and is namediMC535. The predictive potential of the model was validated using previously-reported experimental data. The model predicted the Entner-Duodoroff (ED) pathway to be essential for the growth of this bacterium, whereas the Embden-Meyerhof-Parnas (EMP) pathway was found non-essential. The performance of the model was simulated on various carbon and nitrogen sources and found thatM. capsulatuscan grow on amino acids. The analysis of network topology of the model identified that six amino acids were in the top-ranked metabolic hubs. Using flux balance analysis (FBA), 29% of the metabolic genes were predicted to be essential, and 76 double knockout combinations involving 92 unique genes were predicted to be lethal. In conclusion, we have reconstructed a genome-scale metabolic model of a unique methanotrophMethylococcus capsulatusstr. Bath. The model will serve as a knowledge-base for deeper understanding, as a platform for exploring the metabolic potential, and as a tool to engineer this bacterium for methane mitigation and industrial applications.
Identification of anti-norovirus genes in mouse and human cells using genome-wide CRISPR activation screening
Authors: Orchard Robert C. , Sullender Meagan E. , Dunlap Bria F. , Balce Dale R. , Doench John G. , Virgin Herbert W. 
Published: June 2018

AbstractNoroviruses (NoVs) are a leading cause of gastroenteritis world-wide, yet host factors that restrict NoV replication are not well understood. Here, we use a CRISPR activation (CRISPRa) genome-wide screening to identify host genes that can inhibit murine norovirus (MNoV) replication in either mouse or human cells. Our screens identified with high confidence 57 genes that can inhibit MNoV infection when overexpressed. A significant number of these genes are in interferon and immune regulation signaling networks, but surprising, the majority of the genes identified are not associated with innate or adaptive immunity nor with any antiviral activity. Confirmatory studies of eight of the genes in validate the initial screening data. Mechanistic studies on TRIM7 demonstrated a conserved role of the molecule in mouse and human cells in restricting MNoV in a step of infection after viral entry. Furthermore, we demonstrate that two isoforms of TRIM7 have differential antiviral activity. Taken together these data provide a resource for understanding norovirus biology and demonstrate a robust methodology for identifying new antiviral molecules across cell types and species.Author SummaryNorovirus is one of the leading causes of foodborne illness world-wide. Despite its prevalence, our understanding of norovirus biology is limited due to the difficulty in growing human norovirus in vitro and a lack of an animal model. Murine norovirus (MNoV) is a model norovirus system because MNoV replicates robustly in cell culture and in mice. To identify host genes that can restrict norovirus replication when overexpressed we performed genome-wide CRISPR activation (CRISPRa) screens to induce gene overexpression at the native locus through recruitment of transcriptional activators to individual gene promoters. We found 57 genes could block murine norovirus replication in either mouse or human cells. Several of these genes are associated with classical immune signaling pathways, while many of the molecules we identified have not been previously associated with antiviral activity. Our data is a resource for those studying norovirus and we provide a robust approach to identify novel antiviral genes.
Application of combined MADM methods as MOORA and PSI for ranking of FMS performance factors
Authors: Jain Vineet 
Published: June 2018

Purpose
Due to the increasing demand of customer and competitive market pressure, manufacturing organizations should be modernized in strategies, production operations, processes and its procedures to remain competitive. So, a flexible manufacturing system (FMS) was adopted by the manufacturing system to fight with competitive pressure. The purpose of this paper is to enhance the performance of manufacturing system, with a focus on its factors.

Design/methodology/approach
In this research, the ranking of the performance factor of FMSs is done by using multiple attribute decision-making (MADM) methods as multi-objective optimization on the basis of ratio analysis (MOORA) and preference selection index (PSI). Weights of attributes are defined by the AHP method.

Findings
Ranking of performance factor is done on the basis of six variables which affect three elements of performance of FMS, i.e. productivity, flexibility and quality. MOORA is applied in three ways such as the ratio-based, reference point and full multiplicative method. In the MOORA method, ranking was done considering weights of attributes and also without it. A PSI method is used to find the best factor among the factors. The results of these methodologies, i.e. MOORA and PSI, are same, i.e. productivity is the primary factor in the manufacturing system. The ranking is validated by the result of different methodology used in this research.

Practical implications
This research has evaluated the important factors and performance variables which can enhance the performance of manufacturing organizations. So, the manufacturing persons can focus on these to enhance its performance.

Originality/value
Combined MADM methods, i.e. MOORA and PSI methodologies, are used in this paper to deal with the ranking of performance factors of the FMS considering qualitative characteristics. These approaches show the conversion of a qualitative attribute to quantitative attributes by using fuzzy logic.
Measuring the invisible
Authors: Ying Fei , Tookey John , Seadon Jeff 
Published: June 2018

PurposeConstruction logistics is an essential part of Construction Supply Chain Management for both project management and cost aspects. The quantum of money that is embodied in the transportation of materials to site could be 39–58 per cent of total logistics costs and between 4 and 10 per cent of the product selling price for many firms. However, limited attention has been paid to measure the logistics performance at the operational level in the construction industry. The purpose of this paper is to contribute to the knowledge about managing logistics costs by setting a key performance indicator (KPI) based on the number of vehicle movements to the construction site.Design/methodology/approachA case study approach was adopted with on-site observations and interviews. Observations were performed from the start of construction until “hand-over” to the building owner. A selection of construction suppliers and subcontractors involved in the studied project were interviewed.FindingsData analysis of vehicle movements suggested that construction transportation costs can be monitored and managed. The identified number of vehicle movements as a KPI offers a significant step towards logistics performance management in construction projects.Originality/valueThis research paper demonstrates that framework of using vehicular movements meet the criterion of effective KPI and is able to detect rooms for improvements. The key findings shed valuable insight for industry practitioners in initiating the measurement and monitor “the invisible logistics costs and performance”. It provides a basis for benchmarking that enables comparison, learning and improvement and thereby continuous enhancement of best practice at the operational level, which may accelerate the slow SCM implementation in the construction industry.
Multi-principal collaboration and supplier’s compliance with codes-of-conduct
Authors: Delbufalo Emanuela , Bastl Marko 
Published: June 2018

Purpose
The purpose of this paper is to articulate propositions on how collaborating multi-national corporations (MNCs) can manage their supplier base in order to reduce the risk of suppliers’ non-compliance with shared codes-of-conduct.

Design/methodology/approach
The study utilises a conceptual theory development approach. In doing so, it utilises key tenets of agency theory that are applied in a multi-principal–supplier relationship context and synthesised in a series of propositions.

Findings
The study shows that MNCs have a variety of mechanisms for reducing the risk of suppliers’ non-compliance by decreasing information asymmetry, increasing their bargaining power and simultaneously use of both rewards/sanctions, and reputation-based safeguards.

Research limitations/implications
This is a conceptual theory development study, offering testable propositions, which have then to be empirically validated.

Practical implications
The study showcases that managers of MNCs who find themselves in relationships with non-compliant suppliers have at their disposal a variety of mechanisms to reduce the risk of suppliers’ non-compliance.

Originality/value
This is one of the first studies that explore suppliers’ non-compliance with codes-of-conduct at the level of a relationship, rather than a single firm. In this way it proposes a theoretical framework grounded in agency theory on managing relationships between multi-principal collaborators and their suppliers.
Silver lining of envy on social media? The relationships between post content, envy type, and purchase intentions
Authors: Lin Ruoyun 
Published: June 2018

Purpose
The purpose of this paper is to explore the prevalence of benign and malicious envy on social media, and to examine the relationships between shared content (experiential vs material purchases), envy type (benign vs malicious), and purchase intention (toward the same vs a superior object).

Design/methodology/approach
Three studies (N=622) were conducted to ask participants to recall the last time they experienced envy due to browsing social media, report an envy-triggering post about either an experiential or a material purchase shared by others and read a post about a friend’s newly bought MacBook in either an experiential or a material phrasing. The degrees of benign and malicious envy were measured, as well as the future purchase intentions toward the same and a superior object.

Findings
The results showed that most of the envious emotions were actually benign envy. Although there was no main effect of purchase type on envy type, both experiential purchases and phrasings were less likely to be perceived as showing off, and therefore triggered less malicious envy. Furthermore, benign envy was positively associated with the purchase intention of the same envied purchase, and malicious envy was positively associated with the purchase intention of something even superior.

Originality/value
As browsing other’s social news sometimes evokes envy, people were concerned about the negative effects of envy on consumers. However, this paper addressed the positive effects of envy which comes along with a motivation of moving up. This positive motivation can also be utilized for social media advertising.
Mean reverting leverage policy in China: theory and evidence from industrial and sectorial level unit root analysis
Authors: Rehman Ajid ur 
Published: June 2018

Purpose
This study aims to apply unit root test to investigate the behavior of Chinese firms toward their leverage policy. The study is based on two influential and competing theories of capital structure.

Design/methodology/approach
This study applies unit root test to investigate the behavior of Chinese firms toward their leverage policy. The study is based on two influential and competing theories of capital structure. Trade off theory advocates that firms have a target level of leverage ratio and that firms try to achieve that optimal leverage ratio, whereas pecking order theory argues that firms have no target level of leverage and that they follow a specific pattern of leverage. For this purpose, this study applies a Fisher type unit root test to 12,808 firm level observations. The data are unbalanced and cover a period from 1991 to 2014.

Findings
The results reveal the presence of a stationary behavior across short-term, long-term and total leverage policies. For short-term leverage policy, 21 per cent firms show stationary behavior, while for long-term, 20 per cent show a targeting behavior; for the total leverage policy 17 per cent of firms are found to follow a tradeoff model. To make the findings more interesting sample was further classified into profit and loss making firms. The study finds that loss making firms do not follow a target level of leverage in China. Furthermore, unit root is applied to all firms before and after crises-2008. It is revealed that stationary behavior is more prevalent before crises-2008.

Originality/value
This study is highly important from the point of view that it quantifies firms into distinct categories of following specific model of capital structure. To the best of the author’s knowledge, the findings of this study add to current research knowledge about Chinese firms with respect to adjustment behavior toward a target capital structure.
Advertising skepticism, need for cognition and consumers’ attitudes
Authors: Raziq Muhammad Mustafa , Ahmed Qazi Mohammed , Ahmad Mansoor , Yusaf Saquib , Sajjad Aymen , Waheed Salman 
Published: June 2018

Purpose
The purpose of this paper is to examine the relationship of advertising skepticism and need for cognition with consumers’ attitudes toward brand. There is currently limited understanding on how advertising skepticism and need for cognition relate to the consumers’ attitudes.

Design/methodology/approach
Using a “within-brand-comparison” strategy, a mock print advertisement of a telecom brand is shown to 204 cellular services users in Pakistan. This is followed by a survey. Data are analyzed using a variance-based structural equation modeling.

Findings
The relationship of advertising skepticism with attitude toward brand is negative and partially mediated by the sequence of brand image, advertisement believability and attitudes toward advertisement. In contrast, the relationship between need for cognition and attitude toward brand is positive and fully mediated by the sequence of brand image, advertisement believability and attitudes toward advertisement.

Originality/value
The paper fills some theoretical as well as empirical gaps by showing how (in a within-brand comparative advertisement context) advertising skepticism and need for cognition relate to the consumers’ attitudes toward brand.
Quality of life of head and neck cancer patients in urban and regional areas: An Australian perspective
Authors: Pateman Kelsey A. , Cockburn Nicole L. , Batstone Martin D. , Ford Pauline J. 
Published: June 2018

AbstractObjectiveHead and neck cancer treatment affects quality of life. There are differences in quality of life outcomes and perceived supportive care needs between cancer patients living in metropolitan and regional–remote areas. This study investigated quality of life over the first 6 months following head and neck cancer diagnosis and observed differences in quality of life by geographical location.DesignProspective observational study.SettingTertiary hospital in Brisbane, Australia.ParticipantsNewly diagnosed patients who were referred for the diagnosis, and/or treatment of head and neck cancer.Main outcome measure(s)Quality of life was measured by the University of Washington Quality of Life Survey, version 4 (UW‐QoL). Participants completed the UW‐QoL questionnaire prior to starting treatment, 1 month and 6 months post treatment. Metropolitan and regional or remote status was classified according to the Australian Standard Geographic Classification‐Remoteness Area system.ResultsNinety‐five participants were included at baseline; 49 and 41 participants completed the 1‐month and 6‐month follow‐ups, respectively. Scores in most UW‐QoL domains decreased between baseline and 1 month post treatment and increased towards pre‐treatment scores at the 6‐month follow‐up (except for anxiety and saliva). Pain at baseline was significantly worse in the regional–remote participants compared with metropolitan participants. No other statistically significant differences in UW‐QoL score by geographical location were observed.ConclusionThe findings generally did not support significant differences in quality of life outcome between metropolitan and regional–remote head and neck cancer patients. The difference in pain experience between metropolitan and regional–remote groups requires further investigation.
Neural network model for growth of Salmonella Typhimurium in brain heart infusion broth
Authors: Oscar Thomas P. 
Published: June 2018

SummaryModels that predict growth of Salmonella as a function of variables in the current and previous environment are valuable tools for assessing the safety of food. Therefore, this study was undertaken to develop a model for growth of Salmonella Typhimurium in brain heart infusion broth as a function of previous pH (5.7–8.6), temperature (15–40 °C), pH (5.2–7.4) and time. Viable count data (log CFU mL−1) were modelled using a neural network approach. The variable impacts were 2.4% for previous pH, 29.0% for temperature, 4.9% for pH and 63.7% for time. The proportion of residuals in an acceptable prediction zone (pAPZ) from ‐1 (fail‐safe) to 0.5 log CFU mL−1 (fail‐dangerous) was 0.965 (1061/1100) for dependent data and 0.939 (386/411) for independent data for interpolation. A pAPZ ≥ 0.7 indicated that the model provided predictions with acceptable accuracy and bias. Thus, the model was successfully validated.
A personal perspective on the science, ethics, and commercialization of xenotransplantation
Authors: Rothblatt Martine 
Published: June 2018

AbstractA parent of a child with a fatal lung disease decides to try to save her first with improved pharmaceuticals to hold the disease at bay, and then with an unlimited supply of transplantable lungs to cure the disease and manage the consequences of eventual organ rejection. The parent needs to surmount science, ethics, and commercialization issues for organ transplantation to be a practical cure, and several of those issues are particularly difficult for xenotransplanation. After half‐a‐decade of effort, the parent’s personal journey has resulted in a 100‐fold increase in the number of hours that pulmonary xenografts are viable, but still remains significantly shy of commercial viability and still leaves some ethical issues open.
Regulatory T cells from allo‐ to xenotransplantation: Opportunities and challenges
Authors: Ezzelarab Mohamed B. 
Published: June 2018

AbstractRegulatory T cells (Treg) are currently being evaluated in clinical allotransplantation for tolerance induction, with proven safety in humans with autoimmune diseases and graft‐versus‐host disease. A considerable amount of recent data suggests that additional factors may need to be validated, including the stability and commitment of newly discovered Treg subsets under inflammatory conditions, to further warrant safe and effective Treg‐based therapeutic approaches. This review explores the opportunities and challenges of Treg‐based cell therapy in xenotransplantation. The emerging new technologies for genetic modifications of the donor pig offer a major advantage for Treg therapy to improve xenograft protection. Particularly, the feasibility of (i) ex vivo expansion of donor (pig)‐specific Treg for infusion, and (ii) development of Treg in situ for the life of the xenograft. Our understanding of the Treg biology and their role in xenograft protection, under the newly developed immunosuppressive protocols remains limited. The incidence of various Treg subpopulations in xenograft recipients and their suppressive efficacy across species barriers are largely unknown. Finally, deciphering the dynamics of Treg function, and their interaction with adaptive and innate immune cells are of critical importance to design safe, effective and clinically relevant Treg‐based therapeutic approaches in xenotransplantation.
The 2017 IXA Presidential Lecture: Recent developments in xenotransplantation
Authors: Cowan Peter J. 
Published: June 2018

AbstractThe last few years have seen significant progress in xenotransplantation. Porcine xenograft survival in preclinical models continues to improve, accompanied by the adjustment of immunosuppression to more clinically realistic levels. The rapid uptake of CRISPR/Cas9 technology has accelerated the generation of new knock‐in and knockout pigs, including animals null for the endogenous retrovirus PERV. This brief review presents a personal view of recent developments in the field.
New insights on innate B‐cell immunity in transplantation
Authors: Zorn Emmanuel 
Published: June 2018

AbstractInnate B cells and natural antibodies (Nabs) have been extensively studied in normal physiological conditions as well as in several diseases. However, their significance in the context of ABO‐compatible solid organ transplantation is only emerging. This review summarizes recent studies exploring these often neglected innate immune elements in situations related to sensitization and clinical graft rejection. A focus is placed on class‐switched IgG Nabs that develop amidst inflammation, rather than IgM Nabs abundant at the steady state, as new evidence point to their implication in serum reactivity to HLA and kidney graft failure. The involvement of innate B cells in the pathophysiology of CAV is also presented. Lastly, we discuss key questions that need answering to understand whether and how innate B‐cell immunity contributes to the outcome of solid organ transplantation.