bluuebunny/crossref_metadata_2025_split huggingface dataset:
Konsiliar- und Liaisonpsychiatrie im Allgemeinkrankenhaus
Authors: Bartels P. , Burian R. , Diefenbacher A. , Kratz T. 
Published: September 2018

ZusammenfassungSomatische Erkrankungen werden häufig von psychischen Symptomen und Verhaltensauffälligkeiten begleitet. Andererseits weisen viele körperliche Beschwerden auch auf das Vorliegen einer larvierten psychischen Erkrankung hin. Aus diesem Grund hat in den letzten Jahren die Bedeutung der Konsiliarpsychiatrie und Psychotherapie am Allgemeinkrankenhaus deutlich zugenommen. Der vorliegende Artikel gibt einen Überblick über die Aufgaben und die Organisation der Konsiliarpsychiatrie am Allgemeinkrankenhaus. Besonderer Aspekt wird auf die Bedeutung der Konsiliarpsychiatrie für die Arbeit des somatisch tätigen Arztes gelegt. Es sollten Probleme, Arbeitsfelder und Chancen der Konsiliarpsychiatrie am Allgemeinkrankenhaus aufgezeigt werden.
Outcome after Combined Lateral Extra-articular Tenodesis and Anterior Cruciate Ligament Revision in Professional Soccer Players
Authors: Alessio-Mazzola Mattia , Formica Matteo , Russo Antonio , Sanguineti Francesca , Capello Andrea Giorgio , Lovisolo Stefano , Felli Lamberto 
Published: September 2018

AbstractWe report the functional outcome after combined anterior cruciate ligament (ACL) reconstruction and lateral extra-articular tenodesis (LET) for ACL re-rupture and high-grade pivot shift in professional soccer players. For this retrospective review, the medical records of 24 professional soccer players were analyzed. The mean age at surgery was 23.8 ± 4.2 years and the mean follow-up was 42.2 ± 16.9 months. Pre- and postoperative assessment included the KT-1000 Lachman test, pivot shift test, International Knee Documentation Committee (IKDC) subjective knee evaluation, Tegner activity scale (TAS), and Lysholm score. The rate of return to sports and the level of play at final follow-up were recorded. ACL revision was performed with an autologous bone–patellar tendon–bone autograft or a hamstring graft. LET was performed using an extra-articular MacIntosh procedure as modified by Arnold–Coker. Anterior–posterior laxity was significantly reduced at the final clinical assessment (p < 0.0001): 22 patients (91.7%) had a negative pivot shift and 2 (8.3%) had residual glide (+), with significant improvement (p < 0.0001). The mean subjective IKDC and Lysholm score improved from 69.5 ± 11.1 (range: 56–90) to 88.4 ± 8.9 (range: 62.1–100) and from 58.1 ± 11.7 (range: 33–72) to 97.4 ± 3.2 (range: 88–100), respectively, with significant improvement (p < 0.0001) over preoperative values. The overall failure rate was 8.3%. There were no differences between mean preinjury and final TAS scores (p > 0.05). The rate of return to sports at the same level was 91.7% and the mean time to return to sports was 9.2 ± 2.2 months. Mid-term functional outcome after combined extra-articular reconstruction and ACL revision surgery was satisfactory, with a reduction in residual postoperative rotatory instability and degree of pivot shift.
Is Arthrodesis a Reliable Salvage Option following Two-Stage Revision for Suspected Infection in Proximal Tibial Replacements? A Multi-Institutional Study
Authors: Sambri Andrea , Bianchi Giuseppe , Parry Michael , Frenos Filippo , Campanacci Domenico , Donati Davide , Jeys Lee 
Published: September 2018

AbstractThe aim of this multicentric retrospective study was to verify whether knee arthrodesis (KA) is a viable reconstructive option after two-stage revision for infection of proximal tibia (PT) endoprosthetic reconstruction (EPR). Sixty patients who underwent a two-stage revision were included. Definitive EPR or a KA with a modular system was performed following consideration of soft tissue and extensor mechanism conditions. Patients were evaluated with Musculoskeletal Tumor Society Score and Oxford Knee Score. Implant survival was assessed on the basis of recurrence of infection. Five patients did not receive any reconstruction after the first stage. In 14 cases, a KA was performed, and in 41, an EPR was implanted. At 5 years follow-up, reinfection rate in the KA group was lower (10 vs. 17.5% in KA and EPR groups, respectively). In reinfected patients, the KA group had a reduced rate of amputation when compared with those with EPR (50 vs. 88%). Functional evaluation did not show any significant differences between the two groups. A successful KA using a modular implant can eradicate infection and allow preservation of the limb with good function and good pain relief in after two-stage revision for an infected PT EPR.
Does Chronic Ankle Instability Influence Knee Biomechanics of Females during Inverted Surface Landings?
Authors: Li Yumeng , Ko Jupil , Walker Marika , Brown Cathleen , Schmidt Julianne , Kim Seock-Ho , Simpson Kathy 
Published: September 2018

AbstractThe primary purpose of the study was to determine whether atypical knee biomechanics are exhibited during landing on an inverted surface. A seven-camera motion analysis system and two force plates were used to collect lower extremity biomechanics from two groups of female participants: 21 subjects with chronic ankle instability (CAI) and 21 with pair-matched controls. Subjects performed ten landings onto inverted and flat platforms on the CAI/matched and non-test limbs, respectively. Knee and ankle joint angles, joint angular displacements, joint moments and eccentric work were calculated during the landing phase and/or at the initial contact. Paired t-tests were used to compare between-group differences (p<0.05). We observed that CAI group displayed a significantly increased knee flexion angle, knee flexion displacement, peak knee extension moment and internal rotation moment, and eccentric work in the sagittal plane, possibly due to altered ankle biomechanics. Participants with CAI employed some compensatory strategy to improve their ankle and postural stability during landing onto the tilted surface. The increased knee extension and internal rotation moments of CAI participants could potentially result in a greater ACL loading. In future studies, it may be worthwhile to measure or estimate the ACL loading to confirm whether CAI could relate to the mechanism of ACL injury.
Genomic responses to selection for tame/aggressive behaviors in the silver fox (
Vulpes vulpes
)
Authors: Wang Xu , Pipes Lenore , Trut Lyudmila N. , Herbeck Yury , Vladimirova Anastasiya V. , Gulevich Rimma G. , Kharlamova Anastasiya V. , Johnson Jennifer L. , Acland Gregory M. , Kukekova Anna V. , Clark Andrew G. 
Published: September 2018

Significance
The behavior of domesticated animals differs dramatically from that of wild relatives, and the Russian tame fox experiment demonstrated clearly that these changes can occur in just a few generations of selection. Analysis of gene expression in the brains of tame and aggressive foxes from this experiment allows us to ask what brain pathways have been altered by this recent, strong selection. Pathways that impact the function of both serotonergic and glutaminergic neurons were clearly modulated by selection, consistent with the roles of these neurons in learning and memory. Both allele frequency and gene expression changes also implicate genes important in neural crest cell function, supporting a possible role of neural crest cells in the domestication syndrome.
Two-level masers as heat-to-work converters
Authors: Ghosh Arnab , Gelbwaser-Klimovsky David , Niedenzu Wolfgang , Lvovsky Alexander I. , Mazets Igor , Scully Marlan O. , Kurizki Gershon 
Published: September 2018

Significance
We propose a paradigm of heat-powered maser. In contrast to textbook knowledge, it does not require population inversion or coherent driving and hence can operate with a two-level working medium. Therefore, it is a conceptually different type of maser and, more generally, a conceptually different quantum heat machine. Its autonomous character and “free” power source make this machine technologically enticing.
Intrinsic spin of elastic waves
Authors: Long Yang , Ren Jie , Chen Hong 
Published: September 2018

Significance
Spin unveils the fundamental geometrical and topological properties of waves. Massive studies have shown that the circularly polarized transverse wave can carry nontrivial spin density and thus possess quantum spin Hall effect. However, the spin nature of the longitudinal wave still lacks research. Here, based on the elastic wave platform, we show that the longitudinal wave can carry unique spin angular momentum due to its special spin–orbit coupling, and, importantly, the mixed longitudinal–transverse waves will induce an unusual spin form: hybrid spin. These spins in an elastic wave will be responsible for several spin-related phenomena beyond a pure transverse wave. Our work can advance physical understanding about spin properties of elastic waves.
The Role of Figurative Complexity in the Comprehension and Appreciation of Advertisements
Authors: Pérez-Sobrino Paula , Littlemore Jeannette , Houghton David 
Published: September 2018

AbstractTo date, research in advertising has focussed almost exclusively on metaphor, with linguists and marketing scholars paying very little attention to alternative types of figurative expression. Beyond the finding that metaphor leads to an increased appreciation of advertisements, there has been surprisingly little research into how consumer response is affected by metonymy, or by metaphor–metonymy interactions. In this article, we present findings from a study that investigated the depth to which participants (n = 90) from a range of cultural and linguistic backgrounds (the UK, Spain, and China) were found to process 30 real-world adverts featuring creative metaphor and metonymy in multimodal format. We focus on the cross-cultural variation in terms of time taken to process, appreciation and perceived effectiveness of adverts, and on individual differences explained by different levels of need for cognition. We found significant variation in the understanding of advertisements containing metaphor, metonymy, and combinations of the two, between subjects and across nationalities in terms of (i) processing time, (ii) overall appeal, and (iii) the way in which participants interpreted the advertisements.
Parkour LIMS: high-quality sample preparation in next generation sequencing
Authors: Anatskiy E , Ryan D P , Grüning B A , Arrigoni L , Manke T , Bönisch U 
Published: September 2018

Abstract

Motivation
This paper presents Parkour, a software package for sample processing and quality management of next generation sequencing data and samples.

Results
Starting with user requests, Parkour allows tracking and assessing samples based on predefined quality criteria through different stages of the sample preparation workflow. Ideally suited for academic core laboratories, the software aims to maximize efficiency and reduce turnaround time by intelligent sample grouping and a clear assignment of staff to work units. Tools for automated invoicing, interactive statistics on facility usage and simple report generation minimize administrative tasks. Provided as a web application, Parkour is a convenient tool for both deep sequencing service users and laboratory personal. A set of web APIs allow coordinated information sharing with local and remote bioinformaticians. The flexible structure allows workflow customization and simple addition of new features as well as the expansion to other domains.

Availability and implementation
The code and documentation are available at https://github.com/maxplanck-ie/parkour.

Supplementary information
Supplementary data are available at Bioinformatics online.
Exploring sequence-based features for the improved prediction of DNA N4-methylcytosine sites in multiple species
Authors: Wei Leyi , Luan Shasha , Nagai Luis Augusto Eijy , Su Ran , Zou Quan 
Published: September 2018

AbstractMotivationAs one of important epigenetic modifications, DNA N4-methylcytosine (4mC) is recently shown to play crucial roles in restriction–modification systems. For better understanding of their functional mechanisms, it is fundamentally important to identify 4mC modification. Machine learning methods have recently emerged as an effective and efficient approach for the high-throughput identification of 4mC sites, although high predictive error rates are still challenging for existing methods. Therefore, it is highly desirable to develop a computational method to more accurately identify m4C sites.ResultsIn this study, we propose a machine learning based predictor, namely 4mcPred-SVM, for the genome-wide detection of DNA 4mC sites. In this predictor, we present a new feature representation algorithm that sufficiently exploits sequence-based information. To improve the feature representation ability, we use a two-step feature optimization strategy, thereby obtaining the most representative features. Using the resulting features and Support Vector Machine (SVM), we adaptively train the optimal models for different species. Comparative results on benchmark datasets from six species indicate that our predictor is able to achieve generally better performance in predicting 4mC sites as compared to the state-of-the-art predictors. Importantly, the sequence-based features can reliably and robust predict 4mC sites, facilitating the discovery of potentially important sequence characteristics for the prediction of 4mC sites.Availability and implementationThe user-friendly webserver that implements the proposed 4mcPred-SVM is well established, and is freely accessible at http://server.malab.cn/4mcPred-SVM.Supplementary informationSupplementary data are available at Bioinformatics online.
Biomonitoring an albumin adduct of the cooked meat carcinogen 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine in humans
Authors: Bellamri Medjda , Wang Yi , Yonemori Kim , White Kami K , Wilkens Lynne R , Le Marchand Loïc , Turesky Robert J 
Published: September 2018

Abstract
2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) is formed in cooked meats and may be linked to dietary-associated colorectal, prostate and mammary cancers. Genotoxic N-oxidized metabolites of PhIP react with the Cys34 of albumin (Alb) to form a sulfinamide adduct, a biomarker of the biologically effective dose. We examined the kinetics of PhIP-Alb adduct formation in plasma of volunteers on a 4-week semicontrolled diet of cooked meat containing known quantities of PhIP. The adduct was below the limit of detection (LOD) (10 femtograms PhIP/mg Alb) in most subjects before the meat feeding but increased by up to 560-fold at week 4 in subjects who ate meat containing 8.0 to 11.7 μg of PhIP per 150–200 g serving. In contrast, the adduct remained below the LOD in subjects who ingested 1.2 or 3.0 μg PhIP per serving. Correlations were not seen between PhIP-Alb adduct levels and PhIP intake levels (P = 0.76), the amount of PhIP accrued in hair (P = 0.13), the amounts of N-oxidized urinary metabolites of PhIP (P = 0.66) or caffeine CYP1A2 activity (P = 0.55), a key enzyme involved in the bioactivation of PhIP. The half-life of the PhIP-Alb adduct was &lt;2 weeks, signifying that the adduct was not stable. PhIP-Alb adduct formation is direct evidence of bioactivation of PhIP in vivo. However, the PhIP hair biomarker is a longer lived and more sensitive biomarker to assess exposure to this potential human carcinogen.
Chromosome-level genome assembly of the spotted sea bass, Lateolabrax maculatus
Authors: Shao Changwei , Li Chang , Wang Na , Qin Yating , Xu Wenteng , Liu Qun , Zhou Qian , Zhao Yong , Li Xihong , Liu Shanshan , Chen Xiaowu , Mahboob Shahid , Liu Xin , Chen Songlin 
Published: September 2018

Abstract

Background
The spotted sea bass (Lateolabrax maculatus) is a valuable commercial fish that is widely cultured in China. While analyses using molecular markers and population genetics have been conducted, genomic resources are lacking.

Findings
Here, we report a chromosome-scale assembly of the spotted sea bass genome by high-depth genome sequencing, assembly, and annotation. The genome scale was 0.67 Gb with contig and scaffold N50 length of 31 Kb and 1,040 Kb, respectively. Hi-C scaffolding of the genome resulted in 24 pseudochromosomes containing 77.68% of the total assembled sequences. A total of 132.38 Mb repeat sequences were detected, accounting for 20.73% of the assembled genome. A total of 22, 015 protein-coding genes were predicted, of which 96.52% were homologous to proteins in various databases. In addition, we constructed a phylogenetic tree using 1,586 single-copy gene families and identified 125 unique gene families in the spotted sea bass genome.

Conclusions
We assembled a spotted sea bass genome that will be a valuable genomic resource to understanding the biology of the spotted sea bass and will also lead to the development of molecular breeding techniques to generate spotted sea bass with better economic traits.
Single-cell RNA-seq reveals dynamic transcriptome profiling in human early neural differentiation
Authors: Shang Zhouchun , Chen Dongsheng , Wang Quanlei , Wang Shengpeng , Deng Qiuting , Wu Liang , Liu Chuanyu , Ding Xiangning , Wang Shiyou , Zhong Jixing , Zhang Doudou , Cai Xiaodong , Zhu Shida , Yang Huanming , Liu Longqi , Fink J Lynn , Chen Fang , Liu Xiaoqing , Gao Zhengliang , Xu Xun 
Published: September 2018

Abstract

Background
Investigating cell fate decision and subpopulation specification in the context of the neural lineage is fundamental to understanding neurogenesis and neurodegenerative diseases. The differentiation process of neural-tube-like rosettes in vitro is representative of neural tube structures, which are composed of radially organized, columnar epithelial cells and give rise to functional neural cells. However, the underlying regulatory network of cell fate commitment during early neural differentiation remains elusive.

Results
In this study, we investigated the genome-wide transcriptome profile of single cells from six consecutive reprogramming and neural differentiation time points and identified cellular subpopulations present at each differentiation stage. Based on the inferred reconstructed trajectory and the characteristics of subpopulations contributing the most toward commitment to the central nervous system lineage at each stage during differentiation, we identified putative novel transcription factors in regulating neural differentiation. In addition, we dissected the dynamics of chromatin accessibility at the neural differentiation stages and revealed active cis-regulatory elements for transcription factors known to have a key role in neural differentiation as well as for those that we suggest are also involved. Further, communication network analysis demonstrated that cellular interactions most frequently occurred in the embryoid body stage and that each cell subpopulation possessed a distinctive spectrum of ligands and receptors associated with neural differentiation that could reflect the identity of each subpopulation.

Conclusions
Our study provides a comprehensive and integrative study of the transcriptomics and epigenetics of human early neural differentiation, which paves the way for a deeper understanding of the regulatory mechanisms driving the differentiation of the neural lineage.
A nonsense mutation in myelin protein zero causes congenital hypomyelination neuropathy through altered P0 membrane targeting and gain of abnormal function
Authors: Fratta Pietro , Ornaghi Francesca , Dati Gabriele , Zambroni Desirée , Saveri Paola , Belin Sophie , D’Adamo Patrizia , Shy Michael , Quattrini Angelo , Laura Feltri M , Wrabetz Lawrence 
Published: September 2018

Abstract
Protein zero (P0) is the major structural protein in peripheral myelin, and mutations in the Myelin Protein Zero (Mpz) gene produce wide-ranging hereditary neuropathy phenotypes. To gain insight in the mechanisms underlying a particularly severe form, congenital hypomyelination (CH), we targeted mouse Mpz to encode P0Q215X, a nonsense mutation associated with the disease, that we show escapes nonsense mediated decay and is expressed in CH patient nerves. The knock-in mice express low levels of the resulting truncated protein, producing a milder phenotype when compared to patients, allowing to dissect the subtle pathogenic mechanisms occurring in otherwise very compromised peripheral myelin. We find that P0Q215X does not elicit an unfolded protein response, which is a key mechanism for other pathogenic MPZ mutations, but is instead in part aberrantly trafficked to non-myelin plasma membranes and induces defects in radial sorting of axons by Schwann cells. We show that the loss of the C-terminal Tyr-Ala-Met-Leu motif is responsible for P0 mislocalization, as its addition is able to restore correct P0Q215X trafficking in vitro. Lastly, we show that P0Q215X acts through dose-dependent gain of abnormal function, as wild-type P0 is unable to rescue the hypomyelination phenotype. Collectively, these data indicate that alterations at the premyelinating stage, linked to altered targeting of P0, may be responsible for CH, and that different types of gain of abnormal function produce the diverse neuropathy phenotypes associated with MPZ, supporting future allele-specific therapeutic silencing strategies.
A simple mathematical model to describe antibody-dependent enhancement in heterologous secondary infection in dengue
Authors: Cerón Gómez Miller , Yang Hyun Mo 
Published: September 2018

Abstract
We develop a mathematical model to describe the role of antibody-dependent enhancement (ADE) in heterologous secondary infections, assuming that antibodies specific to primary dengue virus (DENV) infection are being produced by immunological memory. The model has a virus-free equilibrium (VFE) and a unique virus-presence equilibrium (VPE). VFE is asymptotically stable when VPE is unstable; and unstable, otherwise. Additionally, there is an asymptotic attractor (not a fixed point) due to the fact that the model assumes unbounded increase in memory cells. In the analysis of the model, ADE must be accounted in the initial stage of infection (a window of time of few days), period of time elapsed from the heterologous infection until the immune system mounting an effective response against the secondary infection. We apply the results yielded by model to evaluate ADE phenomonon in heterologous DENV infection. We also associate the possible occurrence of severe dengue with huge viremia mediated by ADE phenomenon.
Canonical NF-κB signaling regulates satellite stem cell homeostasis and function during regenerative myogenesis
Authors: Straughn Alex R , Hindi Sajedah M , Xiong Guangyan , Kumar Ashok 
Published: September 2018

Abstract
Skeletal muscle regeneration in adults is attributed to the presence of satellite stem cells that proliferate, differentiate, and eventually fuse with injured myofibers. However, the signaling mechanisms that regulate satellite cell homeostasis and function remain less understood. While IKKβ-mediated canonical NF-κB signaling has been implicated in the regulation of myogenesis and skeletal muscle mass, its role in the regulation of satellite cell function during muscle regeneration has not been fully elucidated. Here, we report that canonical NF-κB signaling is induced in skeletal muscle upon injury. Satellite cell-specific inducible ablation of IKKβ attenuates skeletal muscle regeneration in adult mice. Targeted ablation of IKKβ also reduces the number of satellite cells in injured skeletal muscle of adult mice, potentially through inhibiting their proliferation and survival. We also demonstrate that the inhibition of specific components of the canonical NF-κB pathway causes precocious differentiation of cultured satellite cells both ex vivo and in vitro. Finally, our results highlight that the constitutive activation of canonical NF-κB signaling in satellite cells also attenuates skeletal muscle regeneration following injury in adult mice. Collectively, our study demonstrates that the proper regulation of canonical NF-κB signaling is important for the regeneration of adult skeletal muscle.