bluuebunny/crossref_metadata_2025_split huggingface dataset:
AKK-derived outer membrane vesicles alleviate indomethacin-induced mucin secretion reduction in LS174T cells by inhibiting endoplasmic reticulum stress
Authors: Zhang Lijun , Ma Shuang , Liang Huixi , Chen Xin , Zhao Jingwen 
Published: November 2024

IntroductionAKK-derived outer membrane vesicles (AKK-OMVs) have shown potential in modulating intestinal mucosal immunity by increasing the number of intestinal goblet cells. However, it remains unclear whether AKK-OMVs can directly regulate MUC2 secretion in goblet cells exposed to indomethacin in vitro and the underlying mechanisms involved.MethodsThe abnormal mucin secretion model in LS174T cells was established using indomethacin, with treatment including Akkermansia muciniphila (AKK) supernatant, AKK-OMVs, and extracellular vesicle removal supernatant. The effects of these treatment on MUC2 expression were observed. Transcriptomic sequencing analysis was used to explore the underlying regulatory mechanisms, which were further validated through qRT-PCR and western blotting.ResultsThe treatment with AKK supernatant and AKK-OMVs alleviated the indomethacin-induced reduction in MUC2 secretion in goblet cells. Mechanistically, transcriptomic analysis showed that the gene expression associated with endoplasmic reticulum (ER) stress were upregulated after indomethacin treatment in LS174T cells. This suggests that AKK-OMVs, as the active component in the supernatant, improved MUC2 expression by inhibiting ER stress.ConclusionAKK-OMVs can directly stimulate goblet cells to promote MUC2 secretion, providing potential for further in vivo studies to confirm their protective effects against indomethacin-induced intestinal injury.
Association between ideal cardiovascular health and bowel conditions among US adults
Authors: Wang Yiwen , Wang Zhigang 
Published: November 2024

ObjectiveThe aim of this study is to explore the relationship between ideal cardiovascular health (CVH), as assessed using the Life’s Essential 8 (LE8), and bowel conditions.MethodsThis cross-sectional study selected 11,108 participants aged ≥20 years from 2005 to 2010 National Health and Nutrition Examination Survey. LE8 scores (range 0–100) were measured according to American Heart Association definitions and were divided into health behavior and health factor scores. Bowel conditions including chronic diarrhea, constipation, and fecal incontinence were diagnosed by the Bowel Health Questionnaire. Weighted logistic regression and restricted cubic spline models were used for correlation analysis.ResultsLogistic regression results showed that LE8 scores were negatively associated with chronic diarrhea and fecal incontinence, but the difference with chronic constipation was not statistically significant. The health behaviors subscale was also negatively correlated with chronic diarrhea, chronic constipation, and fecal incontinence, but health factors were negatively related to chronic diarrhea and fecal incontinence and positively related to chronic constipation. The RCS was consistent with the trend of the logistic regression findings. Sensitivity analyses reconfirmed these outcomes.ConclusionLE8 is highly associated with chronic diarrhea and fecal incontinence, not with chronic constipation. Encouraging optimization of CVH levels may be beneficial for bowel disorders, and prevention of bowel disorders may enhance CVH.
Association between serum carotenoids and hyperuricemia: a cross-sectional study based on NHANES 2001–2006
Authors: Chen Pengfei , Miao Lina , Zhang Lixiao , Du Jianpeng , Guo Ming , Shi Dazhuo 
Published: November 2024

PurposeThis study aims to investigate the association between serum carotenoids and hyperuricemia.MethodsData were sourced from the National Health and Nutrition Examination Survey (NHANES) between 2001 and 2006. Hyperuricemia was defined as serum uric acid (UA) levels of ≥7 mg/dL for males and ≥ 6 mg/dL for females. Serum carotenoid levels were measured using high-performance liquid chromatography. Multivariate linear regression was used to analyze the correlation between serum carotenoids and UA levels. Multivariate logistic regression and restricted cubic spline (RCS) analyses were performed to explore the potential association between serum carotenoids and hyperuricemia. Additionally, subgroup and interaction analyses were conducted to determine variations across different population groups.ResultThis cross-sectional study included 13,561 participants. Multivariate linear regression analysis revealed that higher levels of serum carotenoids were correlated with lower UA levels. Specifically, the beta coefficients (β) and 95% confidence intervals (CIs) were as follows: α-carotene (−0.23 [−0.31, −0.15]), β-carotene (−0.30 [−0.38, −0.21]), β-cryptoxanthin (−0.17 [−0.25, −0.09]), lutein/zeaxanthin (−0.12 [−0.20, −0.04]), and total serum carotenoids (−0.25 [−0.33,-0.16]). However, lycopene showed no significant correlation with UA (−0.01 [−0.09, 0.08]). Multivariate logistic regression analysis indicates a significant inverse association between serum carotenoids and the risk of hyperuricemia. The odds ratios (ORs) and 95%CIs were as follows: α-carotene (0.61 [0.49, 0.77]), β-carotene (0.67 [0.51, 0.86]), β-cryptoxanthin (0.69 [0.51, 0.88]), lutein/zeaxanthin (0.72 [0.56, 0.97]), lycopene (0.82 [0.67, 1.00]) and total serum carotenoids (0.73 [0.57, 0.92]). RCS analysis indicated a potential nonlinear relationship between lycopene and hyperuricemia, with an inflection point at 33.45 μg/dL. Subgroup and interaction analyses demonstrated that the inverse association remained consistent across various demographic groups.ConclusionThis study found that higher serum carotenoid levels are associated with lower UA levels and reduced risk of hyperuricemia. Notably, while lycopene was associated with reduced hyperuricemia risk, its effect showed some heterogeneity.
Efficacy of acupuncture combined with mirror therapy in the treatment of post-stroke limb movement disorders: a systematic review and meta-analysis of randomised controlled trials
Authors: Ke Weihao , Cheng Hongxin , Ren Xiaoxuan , Yang Liang , Lai Xiaomin , Wang Zhenyu 
Published: November 2024

ObjectiveTo investigate whether the combination of acupuncture and mirror therapy can improve motor impairment in stroke patients.DesignA systematic review and meta-analysis of randomised controlled trials.Data sourcesCNKI, Wanfang, PubMed, Embase, Vip, web of since, Cochrane database and CBM database.Eligibility criteria for selecting studiesThe included randomized controlled trials compared the efficacy of acupuncture therapy (AT) combined with mirror therapy (MT) against AT, MT, and conventional rehabilitation therapy on limb motor impairment in stroke patients, with independent data extraction and study quality assessment conducted. A META analysis using fixed-effect and random-effect models was performed to calculate the mean difference (MD) in motor scores and the Total effective rate RR (Risk ratio) between the AT combined with MT group and the control group.Main outcome measuresThe Fugl-Meyer Assessment (FMA) for motor function includes the FMA-T (total FMA), FMA-UE (upper extremity FMA), and FMA-L (lower extremity FMA).ResultsA total of 42 randomized controlled trials were included, involving 3,340 patients with post-stroke motor impairment. AT combined with MT was more favorable for FMA-UE (mean difference [MD] = 6.67, 95% CI [5.60–7.93], Z = 11.42, P &lt; 0.0001), FMA-L [MD = 3.37, 95% CI (2.99–3.76), Z = 17.31, P &lt; 0.001], and FMA-T [MD = 6.84, 95% CI (5.92–7.77), Z = 14.48, P &lt; 0.001]. The combined AT and MT treatment was more favorable for the Modified Barthel Index (MBI) score in post-stroke motor impairment [MD = 10.82, 95% CI (8.52–13.12), Z = 9.22, P &lt; 0.001]. AT combined with MT was more favorable for the Modified Ashworth Scale (MAS) [MD = −0.34, 95% CI (−0.66 to −0.03), Z = 14.48, P &lt; 0.001]. AT combined with MT was more favorable for the Total effective rate in treating post-stroke motor impairment (relative risk = 1.27, 95% confidence interval [CI] [1.19–1.37], Z = 6.54, P &lt; 0.001).ConclusionsAT combined with MT can effectively improve patients’ motor function and daily living abilities.Systematic Review RegistrationPROSPERO, identifier, CRD42024559992.
Hybrid Origin of ×Leymotrigia bergrothii (Poaceae) as Revealed by Analysis of the Internal Transcribed Spacer ITS1 and trnL Sequences
Authors: Punina Elizaveta O. , Gnutikov Alexander A. , Nosov Nikolai N. , Shneyer Victoria S. , Rodionov Alexander V. 
Published: November 2024

×Leymotrigia bergrothii is a presumed hybrid of Leymus arenarius and Elytrigia repens. This article investigates the hybrid origin and genome composition of this species. These plants are sterile, do not undergo pollination, and do not produce seeds; occasionally, underdeveloped stamens containing abortive pollen grains form in individual spikelets. The karyotype analysis of root meristem cells revealed a diploid chromosome number of 49 in ×L. bergrothii, reported here for the first time. Subsequently, we examined the intragenomic polymorphism of the transcribed spacer ITS1 in several species of Elytrigia, Elymus, Leymus, Hordeum, and Psathyrostachys, and compared the ribotype patterns of these species with those of ×L. bergrothii. It is shown that the St-ribotype variants found in Elytrigia repens and Elytrigia pseudocaesia, as well as the ribotypes of the La family, which dominate in the genome of Leymus arenarius, correspond to major ribotypes in ×L. bergrothii. The ribotypes of the St and La families are present in the nuclear genome of ×L. bergrothii in almost equal proportions. A comparison of intron and exon sequences of the trnL gene in the chloroplast DNA of Leymus arenarius, Elytrigia repens, and ×L. bergrothii showed that this region in ×L. bergrothii is identical or very close to that of Elytrigia repens, suggesting that Elytrigia repens was the cytoplasmic donor to ×L. bergrothii. Thus, our study confirms the hypothesis that this species represents a sterile first-generation hybrid of Leymus arenarius and Elytrigia repens, reproducing vegetatively.
ND-FISH with New Oligo Probes for Chromosome Identification of Cichorium intybus Revealing Karyotypic Variation and Divergence of Asteraceae Species
Authors: Chen Meiling , Jiang Chengzhi , Huang Doudou , Zheng Zhiqiang , Yang Wenzhuo , Li Guangrong , Fu Chun , Liao Hong , Long Wencong , Yang Zujun , Yang Yaojun 
Published: November 2024

Chicory (Cichorium intybus L., 2n = 18), belonging to the Asteraceae family, exhibits significant edible, medicinal, and pasture values. Moderate research has been performed on identifying Chicory species’ chromosomes using fluorescence in situ hybridization (FISH) and C-banding. Detailed karyotype comparisons with chromosome nomenclature have not yet been performed for Chicory and similar species. In this study, the tandem repeats (TRs) were predicted and mapped to chromosomal regions based on released C. intybus L. ASM2352571 genome assembly v1, and then compared to the genome of Lettuce (Lactuca sativa L.). Nine new oligo probes were then developed and employed for karyotypic investigation of endive, Lettuce, and Chicory mitotic metaphase using non-denaturing FISH (ND-FISH). By combining the conserved oligo probes for 5S rDNA and 18S rDNA with the unique ND-FISH signals of new TR-oligo probes, we can develop a high-resolution standard karyotype for the cultivars of Lettuce and Chicory. The occurrence of chromosome structure variations from the natural population of Chicory and Lettuce was also revealed by ND-FISH with multiple oligo probes. The current observation of the karyotype differences and divergences of Lactuca and Cichorium and the genomic research offers crucial information about the Asteraceae family’s genetic diversity, chromosomal dynamics, and evolutionary routes.
Impacto da pandemia por COVID-19 e reflexões sobre a adesão de lactentes ao Palivizumabe em um complexo hospitalar no Ceará
Authors: Ramos David Almeida Martins , Dias Hannah Iorio , Brizeno Mariana de Oliveira , Rodrigues Aracélia Gurgel , Viana Eugenie Desirèe Rabelo Néri 
Published: November 2024

Este estudo tem como objetivo analisar o impacto da pandemia por covid-19 na adesão de lactentes à imunoprofilaxia para infecção pelo vírus sincicial respiratório (VSR) com palivizumabe em uma Maternidade no Ceará e refletir sobre os determinantes da saúde na adesão, sendo um estudo transversal, descritivo, retrospectivo e com abordagem quantitativa, realizado em uma maternidade do Ceará. Os participantes foram em sua maioria, lactentes < 1 ano com nascimento prematuro, idade gestacional ao nascer inferior a 29 semanas e crianças de até 2 anos com doença pulmonar crônica ou cardíaca com repercussão hemodinâmica, residentes no Estado do Ceará e que receberam o palivizumabe entre 2020 e 2023. Foram identificados 284 lactentes, majoritariamente do sexo feminino (52,82%) que eram principalmente da Região de Saúde de Fortaleza (78,81%%), com idade gestacional média de 27 semanas(s) e 5dias (d), peso médio ao nascer de 1,09 kg, e tendo a doença pulmonar crônica como principal diagnóstico (79,23%). As taxas de adesão ao palivizumabe variaram de 38,46% a 86,7%, com uma adesão média de 59,51% e 3,28 doses em média, por paciente, revelando o impacto negativo que a pandemia de covid-19 teve sobre a adesão à imunoprofilaxia para VSR com palivizumabe, como também demonstrou que após o período pandêmico houve a persistência de uma taxa de não adesão, suscitando reflexões sobre a existência de outros fatores de saúde como determinantes da adesão.
A atuação da enfermagem na segurança transfusional sanguínea
Authors: Carvalho Emerson Cardoso , Mourão Fabiana Costa , Freitas Alessandra Ramisse Nunes , Silva Pamela Vitória Ramos da , Antunes Erika Cristina Brasil , Miranda Betânia Portilho , Martins Fernanda Ferreira , Neri Miquelem Cristina da Luz Gomes , Martins Raiane da Silva , Rodrigues Jhon Victor Bahia , Miranda Silene do Socorro , Lopes Mateus dos Reis , Cruz Jacqueline Aviz Marques , Martins Ana Paula Melo , Belfor Eduardo Lima , Santos Pedro Augusto Ramos dos 
Published: November 2024

Introdução:no Brasil, a violência nas guerras acabou sendo o motivo para a inauguração de diversos serviços de hemoterapia. Dessa forma, em 1942, houve a criação do primeiro serviço de hemoterapia e, até 1964, não existiam registros de leis nacionais referentes à sua regulamentação. Objetivo: Identificar o papel da enfermagem na segurança transfusional sanguínea a partir das publicações científicas. Metodologia: O presente estudo trata-se de uma revisão bibliográfica de abordagem qualitativa descritiva. A revisão bibliográfica de abordagem qualitativa descritiva baseia-se em levantamentos de dados em estudos já realizados por teóricos anteriores e pesquisas, a fim de ter a certeza do método a ser trabalhado e se realmente está com o delimento correto. Resultados e Discussões: A partir da leitura e análise dos artigos identificaram-se três categorias temáticas a serem discutidas: O papel do Enfermeiro no ato transfusional, O conhecimento de enfermagem sobre a conduta frente às reações transfusionais, O conhecimento e dificuldades da equipe de enfermagem no processo transfusional. Conclusão: Conclui-se que a atuação na segurança transfusional sanguínea envolve toda a equipe de enfermagem, mas principalmente os enfermeiros que devem estar atentos aos corretos procedimentos de um processo de transfusão, porém, foi observado que há um despreparo técnico e insegurança por parte de alguns membros da equipe de enfermagem.
Levantamento sobre os diferentes métodos de emagrecimento praticados por mulheres em uma academia no Noroeste do Paraná
Authors: Santos Eduarda Granado dos , Vasconcelos Sandra Sayuri Nakamura de 
Published: November 2024

Introdução: A insatisfação com a aparência acaba por levar os indivíduos a uma incessante busca de uma imagem corporal considerada ideal. É muito comum associar ganho de peso com as condições de vida e saúde, ignorando a complexibilidade do processo saúde-doença e seu impacto sobre os considerados “magros”. Objetivo: Identificar métodos de emagrecimento utilizados por mulheres que frequentam a academia no município de Maringá Paraná. Metodologia: Refere-se a um estudo de natureza descritiva e transversal na qual foram entrevistadas mulheres entre 18 e 70 anos que frequentam uma academia, que residem no município de Maringá-PR. A pesquisa foi realizada através da aplicação de um questionário estruturado impresso, que avalia características sociodemográficos e métodos utilizados para o emagrecimento. Resultados: Das 155 mulheres que participaram desta pesquisa, a maioria tinha entre 18 a 25 anos, raça branca, solteiras, sem filhos, com renda familiar superior a seis salários mínimos e nível de escolaridade correspondente ao ensino médio. Dentre as entrevistadas, a faixa etária predominante no uso de medicamentos para emagrecer é de 18 a 25 anos (28%), sendo a sertralina o medicamento mais utilizado. Além disso, (78%) das entrevistadas relataram apresentar ansiedade e insatisfação com o próprio corpo, enquanto 18% afirmam fazer uso de medicamentos com o objetivo de melhorar a aparência física. Conclusão: Destaca-se o impacto que a sociedade impõe com relação ao corpo ideal, o que leva a uma busca incessante pela aparência perfeita, resultando no crescente uso de medicamentos e procedimentos estéticos.
Rural Women’s Engagement in Groundnut Production in the Wa East District, Ghana
Authors: Adams Abdulai 
Published: November 2024

In many rural communities, women face barriers to land ownership, access to productive resources such as finance and extension services, and participation in decision-making. These constraints result in the low productivity of women farmers. This study sought to examine the factors influencing women’s engagement in groundnut production and the challenges they face in the Wa East district. The survey data that the study relied on was collected from 240 respondents and analysed using a probit model and Kendall’s Coefficient of Concordance. The results revealed that socio-economic, environmental and institutional factors have impact on women’s engagement in groundnut production. More specifically, the age of the farmer, marital status, household size, access to land, and motivation for income significantly influenced women’s engagement in groundnut production. Furthermore, access to credit, climate change, and lack of farm inputs and machinery are the key constraints facing women farmers engaged in the groundnut production enterprise. Policies that minimize these constraints such as the provision of credit facilities, input support schemes, and farmer training on climate change need to be pursued by the department of agriculture, private companies, and non-governmental organizations working in the agricultural sector. This study contributes to the international literature by highlighting the constraints facing rural women in agricultural production and the drivers of inclusion in farming systems.
TRANSREA
Authors: Saletes Josselin , Grouard Delphine , Puisset Florence , Testier Jessica , Guillarme Séverine , Plumail Jean-François 
Published: November 2024

Introduction et objectif : Les transmissions sont un impératif du rôle propre infirmier et aide-soignant pour la traçabilité de leurs actes, mais surtout dans le souci d’assurer une continuité des soins optimale. Depuis l’avènement de l’écrit dans ces professions, le canal oral et le canal scripturaire sont traditionnellement utilisés au cours des passages de relai entre équipes. Dans un service spécifique comme la réanimation et dans ce climat de bouleversement que nous traversons, ne serait-il pas légitime de réinterroger ce paradigme ?
Matériels et méthode : Une réanimation polyvalente de 17 lits et une unité de surveillance continue de 13 lits fonctionnant en 12h, utilise parallèlement les deux canaux traditionnels de transmission : oral et écrit. Ce modèle entraine une organisation complexe pour un rendu jugé peu qualitatif et très chronophage. Avec la rédaction d’une macrocible d’admission, la création d’une feuille dite « tremplin », la rédaction de transmissions ciblées et l’élaboration d’une synthèse intermédiaire hebdomadaire, un modèle sans transmissions orales a été mis en place.
Résultats : Cinq ans après cette mise en place 100% des IDE et AS sont satisfaits par ce format.
Conclusion : Encadrement et paramédicaux ont largement adopté cette formule et ont relevé de nombreuses plus-values qualitatives et un bénéfice temps certain.
Characteristics of the GLLE Family of Lifetime Distributions, and Applications
Authors: Gleaton James U. , Hamid Sami , Sa Ping 
Published: November 2024

ABSTRACT. A Generalized Log-Logistic (GLL) family of lifetime distributions is one in which any pair of distributions are related through a GLL transformation, for some (non-negative) value of the transformation parameter (the odds function of the second distribution is the power of the odds function of the first distribution). We consider GLL families generated from an exponential distribution. We derive some useful characteristics of these distributions; moreover, we discuss the hazard rates and Kullback-Leibler divergence, then illustrate the usefulness of this distribution family by fitting real data sets.
Phenotypic, Molecular Identification and Virulence Assessment of Cronobacter spp Isolated from Clinical Samples of Children Under Two Years in Mosul City/Iraq
Authors: Alumary Muntaha , Almola Shakir 
Published: November 2024

Cronobacter spp. is known to be a foodborne causative agent for a variety of diseases in both humans and animals. This study focused on isolating Cronobacter species from 150 clinical samples of children under two years (60 from blood and stool samples, 30 from CSF samples) collected from Ibn al Atheer and Al Khanssa Hospitals. The phenotypic identification of bacterial isolates were performed through culture, microscopic, and biochemical examinations and vitek-2 system. The results revealed a mixture of bacteria in 6.5% of the clinical specimens. The identity of isolates was 99% using the vitek-2 system for identifying Cronobacter sakazakii, Cronobacter malonaticus, Cronobacter muytjensii, and Cronobacter pulveris. Out of 150 specimens there were 8 (5.33 %) of specimens gave positive for Cronobacter spp which included: C. sakazaki 2/60 blood specimens (3.3%), C. sakazaki 2/60 stool specimens (3.3%), C. malonaticus 2/60 stool specimens (3.3%), 1/60 C. muytjensii and C. pulveris 1/60 (3.3%). The eight isolates phenotypically identified as Cronobacter were confirmed at the molecular level through 16S rRNA sequencing and submitted to NCBI under the accession numbers (OR825874, OR825875, PP126443, PP126444, PP126445, PP126455). The virulence profile of these isolates showed that 7/8 (87.5%) of Cronobacter strains exhibited haemolysin activity, 5/8 strains (62.5%) were able to produce the protease enzyme and 2/8 (25%) of Cronobacter strains were positive for lipase and lecithinase. All strains lacked the ability to produce a slime layer. The results also showed the ability of Cronobacter strains to produce biofilm by using the tube method and microtiter plate method but with different levels.