bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel method to assess photobiomodulation in stimulating regenerative capacity and vascularization in zebrafish
Authors: Adly Afnan Sedky , Egea Jean‐Christophe , Adly Mahmoud Sedky , Panayotov Ivan , Adly Aya Sedky , Malthiery Eve , Cuisinier Frederic 
Published: November 2024

AbstractPhotobiomodulation (PBM) therapy is a continuously growing approach to stimulating healing and reducing inflammation and pain. However, its effects in the fields of regenerative medicine and tissue engineering are still under investigation. Studying PBM effects on the regenerative capacity of zebrafish can allow the application of novel clinical approaches where the impact of PBM will be cross‐linked with the stem‐cell therapeutic approaches. This study was done to establish an in‐vivo experimental setup for studying the effects of laser and ultraviolet therapy on zebrafish caudal‐fin regeneration and vascularization. Thirty zebrafish were randomly and equally allocated into three groups. The caudal‐fins of all zebrafish were amputated under anaesthesia. In the first control group, the caudal‐fin was only monitored until fully regenerated. In the second group, the amputated‐fin was irradiated with ultraviolet. Finally, in the third group, the amputated‐fin was irradiated with laser. Caudal‐fin regeneration and vascularization were assessed at days 0, 3, 6, 9, 12, and 15 in all fish. In terms of regeneration, the results indicated that it is possible to discriminate the regenerative effect of laser with the experimental setup as laser therapy showed a statistically significant difference when compared to control‐group. It was also found that regenerative stimulation of the group that received ultraviolet therapy showed significant difference when compared to the control group. In terms of vascularization, there was a statistically significant difference in all groups of the study, which may suggest that laser as well as ultraviolet have limited effects in terms of improving vascularization. This study presented a novel, simple and inexpensive method for the assessment of PBM effects on zebrafish. Laser and ultraviolet therapy appeared to act as regenerative stimulators for caudal‐fin regeneration of zebrafish. However, laser therapy results were, to some extent, better than ultraviolet therapy. This novel in‐vivo design of the experiment led to more rapid and reproducible results than in‐vitro experiments.
Pannexin 1 and pannexin 3 differentially regulate the cancer cell properties of cutaneous squamous cell carcinoma
Authors: O'Donnell Brooke L. , Johnston Danielle , Bhatt Ayushi , Kardan Zahra , Stefan Dan , Bysice Andrew , Sayedyahossein Samar , Dagnino Lina , Cecchini Matthew , Loganathan Sampath Kumar , Roth Kathryn , Penuela Silvia 
Published: November 2024

AbstractPannexin (PANX) channels are present in skin and facilitate the movement of signalling molecules during cellular communication. PANX1 and PANX3 function in skin homeostasis and keratinocyte differentiation but were previously reduced in a small cohort of human cutaneous squamous cell carcinoma (cSCC) tumours compared to normal epidermis. In our study we used SCC‐13 cells, limited publicly available RNA‐seq data and a larger cohort of cSCC patient‐matched samples to analyse PANX1 and PANX3 expression and determine the association between their dysregulation and the malignant properties of cSCC. In a bioinformatics analysis, PANX1 transcripts were increased in cSCC and head and neck SCC tumours compared to normal tissues, but PANX3 mRNA showed no differences. However, in our own cohort PANX3 transcripts were decreased in cSCC compared to patient‐matched aged skin, whereas PANX1 protein was upregulated in cSCC. PANX1 localized to all regions within the cSCC tumour microenvironment, and increased levels were associated with larger tumour dimensions. To investigate PANX1 function in SCC‐13 cells, we deleted PANX1 via CRISPR/Cas9 and treated with PANX1 inhibitors, which markedly reduced cell growth and migration. To assess PANX3 function in cutaneous carcinogenesis, we employed the 7,12‐dimethylbenz(a)anthracene/12‐otetradecanoylphorbol‐13‐acetate (DMBA/TPA) model using our global Panx3 knockout (KO) mice, where 60% of wild‐type and 100% of KO mice formed precancerous papillomas. Average papilloma volumes at endpoint were significantly increased in KO mice and showed moderate evidence of increases in KO mice over time. Collectively, these findings suggest PANX1 and PANX3 dysregulation may have potential tumour‐promoting and tumour‐suppressive effects for keratinocyte transformation, respectively.
imageKey points
Pannexin 1 (PANX1) and pannexin 3 (PANX3) are channel‐forming proteins which are critical in the normal maintenance and function of keratinocytes in the skin but may become altered in cutaneous squamous cell carcinoma (cSCC) tumours.
In this study we used a combination of culture models, mouse models and patient‐derived tissues. We found PANX1 levels are increased in cSCC tumours and present in all tumour regions, functioning to promote cSCC cell growth and migration.
Conversely, PANX3 levels are decreased in cSCC tumours, and this protein reduces the incidence and growth of precancerous lesions.
Taken together our data indicate that in cSCC these pannexin family members seem to have opposite effects, in either promoting or restricting cancer cell properties.
These results help us to better understand the mechanisms of malignant transformation of keratinocytes and offer a new potential therapeutic target for the treatment of advanced cSCC.
Orbitofrontal control of the olfactory cortex regulates olfactory discrimination learning
Authors: Wang Ding , Zhang Ying , Li Shan , Liu Penglai , Li Xiang , Liu Zhiqiu , Li Anan , Wang Dejuan 
Published: November 2024

AbstractServing as an integral node for cognitive processing and value‐based decision‐making, the orbitofrontal cortex (OFC) plays a multifaceted role in associative learning and reward‐driven behaviours through its widespread synaptic integration with both subcortical structures and sensory cortices. Despite the OFC's robust innervation of the olfactory cortex, the functional implications and underlying mechanisms of this top‐down influence remain largely unexplored. In this study, we demonstrated that the OFC formed both direct excitatory and indirect inhibitory synaptic connections with pyramidal neurons in the anterior piriform cortex (aPC). OFC projection predominantly regulated spontaneous and odour‐evoked excitatory activity in the aPC of awake mice. Importantly, suppression of this OFC–aPC projection disrupted olfactory discrimination learning, potentially due to a consequent decrease in the excitability of aPC principal output neurons following inhibition of this projection. Whole‐cell recordings revealed that olfactory learning increased the intrinsic excitability of aPC neurons while concurrently decreasing OFC input to these neurons. These findings underscore the pivotal influence of orbitofrontal modulation over the olfactory cortex in the context of olfactory learning and provide insight into the associated neurophysiological mechanisms.
imageKey points
The orbitofrontal cortex (OFC) densely innervates the anterior piriform cortex (aPC) through direct excitatory synaptic connections.
The OFC regulates both spontaneous and odour‐evoked excitatory activities in the aPC of awake mice.
Inhibition of OFC projections disrupts olfactory discrimination learning, probably due to reduced excitability of aPC main output neurons.
Following olfactory learning, the intrinsic excitability of aPC neurons increases while the OFC–aPC input decreases, highlighting the importance of adaptable OFC input for olfactory learning.
These results provide new perspectives on how the OFC's top‐down control modulates sensory integration and associative learning.
Late‐gestation maternal undernutrition induces circulatory redistribution while preserving uteroplacental function independent of fetal glycaemic state
Authors: Cho Steven K.S. , Darby Jack R.T. , Saini Brahmdeep S. , Holman Stacey L. , Lock Mitchell C. , Perumal Sunthara Rajan , Williams Georgia K. , Macgowan Christopher K. , Seed Mike , Morrison Janna L. 
Published: November 2024

AbstractProgramming effects of maternal undernutrition on fetal metabolic and cardiovascular systems are well elucidated, yet a detailed characterization of maternal haemodynamics is not available. This study used comprehensive cardiovascular magnetic resonance (CMR) imaging to quantify maternal haemodynamics after 29 days (111–140 days) of late‐gestation undernutrition (LGUN) in pregnant sheep. Control ewes received 100% of metabolizable energy requirements (MERs, n = 15), whereas LGUN ewes were globally nutrient restricted to 50% MER (n = 18), with a subset of fetuses undergoing continuous glucose infusion (LGUN + G, n = 6/18). Ewes underwent CMR (138–140 days’ gestation), and placental tissue was collected the next day. Ewes in both LGUN groups had reduced body weight and mean blood glucose concentration across gestation. Ventricular dimensions were lower in both LGUN groups. Uterine artery blood flow (QUtA) was elevated in the LGUN group compared with controls, whereas peripheral blood flow was reduced and further diminished in LGUN + G. Maternal weight change correlated with all haemodynamic parameters across all groups. Uteroplacental oxygen and glucose delivery were increased in LGUN compared to control ewes, whereas uteroplacental oxygen consumption was preserved. LGUN did not impact placental or fetal weight, and markers of brain‐sparing physiology were absent. Placental expression of insulin‐like growth factors (IGF‐1 and IGF‐2) and their receptors, glucose, fatty acid (FA) or amino acid transporters and markers of angiogenesis was not impacted. FA transporter expression was positively correlated with QUtA, and FA binding protein correlated negatively with maternal weight change. Maternal cardiovascular adaptations in response to LGUN manifest as preservation of placental growth and function, thereby preserving fetal growth.
imageKey points
Maternal undernutrition during pregnancy alters fetal metabolic and cardiovascular physiology, but little is known about alterations in maternal haemodynamics.
Late‐gestation undernutrition (LGUN) and LGUN + G redirected maternal blood flow from the periphery to the uteroplacental unit, concomitantly increasing the delivery of glucose and oxygen to the uteroplacental unit.
Substrate transporter expression and uteroplacental oxygen consumption were preserved in LGUN and LGUN + G, suggesting prioritization of the placenta.
This study is the first to report detailed maternal haemodynamics in the setting of maternal undernutrition, where placental growth and function were maintained, ultimately preserving fetal oxygen metabolism and growth.
AR-DAVID: Augmented Reality Display Artifact Video Dataset
Authors: Chapiro Alexandre , Kim Dongyeon , Asano Yuta , Mantiuk Rafał K. 
Published: November 2024

The perception of visual content in optical-see-through augmented reality (AR) devices is affected by the light coming from the environment. This additional light interacts with the content in a non-trivial manner because of the illusion of transparency, different focal depths, and motion parallax.
To investigate the impact of environment light on display artifact visibility (such as blur or color fringes), we created the first subjective quality dataset targeted toward augmented reality displays. Our study consisted of 6 scenes, each affected by one of 6 distortions at two strength levels, seen against one of 3 background patterns shown at 2 luminance levels: 432 conditions in total. Our dataset shows that environment light has a much smaller masking effect than expected. Further, we show that this effect cannot be explained by compositing of the AR-content with the background using optical blending models. As a consequence, we demonstrate that existing video quality metrics perform worse than expected when predicting the perceived magnitude of degradation in AR displays, motivating further research.
Non‐surgical Treatment May be Appropriate for Most Chinese Children With Monogenic Congenital Hyperinsulinism Based on a Retrospective Study of 121 Patients
Authors: Cheng Ming , Su Chang , Wang Dongmei , Song Yanning , Li Yang , Zeng He , Yuan Zheng , Li Xiaoqiao , Meng Xi , Ding Yuan , Cao Bingyan , Gong Chunxiu 
Published: November 2024

Objective: There is a notable absence of extensive Chinese studies involving monogenic congenital hyperinsulinism (CHI). The purpose of this large retrospective Chinese cohort with monogenic CHI from a national children’s medical center was to analyze the genetic and clinical characteristics.Methods: We compared clinical characteristics grouped by genotypes based on CHI‐targeted next‐generation sequencing (tNGS) and performed subgroup analyses by onset time.Results: Totally, 121 non‐consanguineous patients were enrolled. Among them, 79 patients (65.3%) had variants in ATP‐sensitive potassium channel (KATP) genes (62 heterozygotes and 17 compound heterozygotes), 35 (28.9%) in glutamate dehydrogenase 1 (GLUD1), and 7 (5.8%) in rare genes (hydroxyacyl‐CoA dehydrogenase [HADH], glucokinase [GCK], and hepatocyte nuclear factor 4 alpha [HNF4A]). Ten patients had ATP binding cassette subfamily C member 8 (ABCC8) variants (p.G111R), and 12 had GLUD1 variants (p.S498L), suggesting two potential founder variants. Three ABCC8 variants (p.G1478R, p.L580_S581insFASL, and p.S986 ∗) and two HNF4A variants (p.R63W and p.V382I) were previously reported to be associated with diabetes. Non‐surgical treatment was effective in 65.9% of patients with KATP variants, while in 100% of those with non‐KATP variants. For the subgroup of KATP variants, neonatal‐onset patients tended to present with mild symptoms (67.9% versus 19.3%), had a higher proportion of surgical intervention (24.5% versus 3.8%), and displayed higher levels of serum insulin and C‐peptide than non‐neonatal onset ones (p < 0.001).Conclusion: The absence of homozygous variants in KATP genes and a quite higher proportion of GLUD1 variants than previous cohorts, may explain a high response rate of non‐surgical treatment in this study. Surgery might be considered for neonatal‐onset children, especially when KATP variants were discovered but not for those carried variants reported to cause diabetes in later life. While expanding the genotypic spectrum, we also highlight the clinical significance of genetic screening.
The Impact of Perceived Emotional Intelligence on Occupational Stress Among Nurses: Empirical Evidence From a Saudi Health Cluster
Authors: Alsufyani Abdulaziz M. , Almalki Mohammed S. , Khader Khaled A. , Stanford Penelope , Freeman Samantha , Alsufyani Yasir M. 
Published: November 2024

Background: Occupational stress is a significant challenge for healthcare systems worldwide. It compromises the quality of healthcare and jeopardizes patient safety. Globally, the estimated economic impact of occupational stress in the healthcare system ranges from US $221.13 million to US $187 billion. Emotional intelligence has been recognized as a behavioral buffer against occupational stress. Hence, this study investigated whether nurses’ perceptions of emotional intelligence impact their self‐perceived occupational stress.Design: A predictive correlational design was utilized.Method: A prior power analysis using G ∗ Power 3.1 was conducted. A convenience sample of 734 nurses was recruited from Taif Health Cluster. Data were coded and analyzed using IBM® SPSS® Statistics for Windows v.25. Descriptive and inferential statistics were used. A hierarchical regression technique was used. The level of significance was established at p < 0.05. The process of data collection started at August 2022 and continued through October 2022.Results: The results indicated that nurses’ age and working area predicted occupational stress perceptions in the first model (β = −0.28 and β = 0.21, p = 0.001, respectively). The second model showed a significant improvement (ΔF(7,727) = 162.35, p < 0.000, ΔR2 = 0.226) and indicated a negative correlation between nurses’ perceptions of emotional intelligence and occupational stress (β = −0.45, t = −12.8, p < 0.000). The nurses’ sociodemographic characteristics in the first model explained 2.4% of the variance. The second model represented 25.0% of the variance when nurses’ emotional intelligence perception was included.Conclusion: Our study shows a novel framework that indicates a positive perceived effect of emotional intelligence on nurses’ perceptions of occupational stress in Saudi Arabia. Our findings propose that emotional intelligence is a significantly effective mechanism against occupational stress.
Association of Lipoprotein(a) With Changes in Coronary Atherosclerosis in Patients Treated With Alirocumab
Authors: Koskinas Konstantinos C. , Häner Jonas , Ueki Yasushi , Otsuka Tatsuhiko , Lonborg Jacob , Shibutani Hiroki , Kakizaki Ryota , Kaiser Christoph , van Geuns Robert-Jan , Ondracek Anna S. , Praz Fabien , Ambühl Maria , Spirk David , Lanz Jonas , Daemen Joost , Heg Dik , Mayr Manuel , Mach François , Windecker Stephan , Engstrøm Thomas , Lang Irene M. , von Eckardstein Arnold , Losdat Sylvain , Räber Lorenz 
Published: November 2024

BACKGROUND:
Elevated Lp(a) (lipoprotein[a]) is a risk marker for atherosclerotic disease, but the underlying mechanisms remain elusive. We examined the association of Lp(a) with changes in coronary atherosclerosis following intensive lipid-lowering therapy.

METHODS:

In the PACMAN-AMI trial (Effects of the PCSK9 Antibody Alirocumab on Coronary Atherosclerosis in Patients With Acute Myocardial Infarction), 300 patients with acute myocardial infarction were randomized to receive biweekly alirocumab 150 mg or placebo in addition to high-intensity statins. Patients underwent serial 2-vessel intravascular ultrasound, optical coherence tomography, and near-infrared spectroscopy in the non–infarct-related arteries at baseline and after 52 weeks. The main end points were percent atheroma volume by intravascular ultrasound, minimum fibrous cap thickness by optical coherence tomography, and maximum lipid core burden index within 4 mm (maxLCBI
4mm
) by near-infrared spectroscopy.

RESULTS:

A total of 265 patients had serial intravascular ultrasound data (mean age, 58±9 years; 16% women). Alirocumab resulted in greater reductions in percent atheroma volume and maxLCBI
4mm
, as well as a greater increase in minimum fibrous cap thickness, compared with placebo. In the alirocumab group, the reduction in maxLCBI
4mm
was smaller in patients with higher baseline Lp(a), defined by the highest quartile (Q4, ≥98 nmol/L; n=30), than in those with lower baseline Lp(a) (Q1–Q3, <98 nmol/L; n=99; −40.2 [−91.1 to 10.7] versus −91.4 [−113.9 to −68.9], respectively;
P
=0.01 after adjustment for clinically relevant baseline variables), and was comparable to the maxLBI
4mm
reduction in the placebo group (−37.60 [−57.40 to −17.80]; n=134). These findings were consistent when higher baseline Lp(a) was defined by cut-off values of ≥75 versus <75 nmol/L (n=35 versus 94, respectively, in the alirocumab group) and ≥125 versus <125 nmol/L (n=23 versus 106, respectively). Changes in percent atheroma volume and minimum fibrous cap thickness did not differ in relation to baseline Lp(a).

CONCLUSIONS:
In patients with acute myocardial infarction, elevated Lp(a) at baseline is associated with attenuation of plaque lipid regression despite intensive treatment with alirocumab plus high-intensity statin. This finding may explain the residual cardiovascular risk associated with high Lp(a) despite optimal control of lipid levels.

REGISTRATION:

URL:
https://www.clinicaltrials.gov
; Unique identifier: NCT03067844.
International Comparison of Quality Indicators for Adults Hospitalized for Heart Failure: A Systematic Review
Authors: Garcia Acevedo Giliana , Ahmad Aisha , Stall Benjamin , Mokhtarnia Media , Lapp John M. , Verma Amol A. , Ebrahim Jalal , Van Spall Harriette G.C. , Razak Fahad , Isenberg Sarina R. , Etchells Edward , Mak Susanna , Steinberg Leah , Ko Dennis T. , Poon Stephanie , Quinn Kieran L. 
Published: November 2024

BACKGROUND:
There is limited international agreement on defining care quality for the millions of people hospitalized with heart failure worldwide. Our objective was to compare and measure agreement across existing internationally published quality indicators (QIs) for the care of adults hospitalized for heart failure.

METHODS:
Systematic review and evidence gap map of internationally published articles reporting on QIs for adults hospitalized for heart failure, using PubMed, MEDLINE, EMBASE, and TRIP from inception to July 18, 2022. Narrative synthesis and descriptive statistics characterized included articles and QIs using the Donabedian Framework of Structural, Process, and Outcomes. The methodological quality of QI sets was assessed using the Appraisal of Indicators through Research and Evaluation instrument. Agreement about QIs was defined as having at least 3 different cardiovascular societies recommend its use. An evidence gap map displayed each QI according to its clinically relevant category, methodological quality, and reporting articles.

RESULTS:
Fourteen articles from 11 societies reported 75 unique QIs; 53 QIs were process, 16 were structural, and 7 were outcome measures. There was limited agreement on individual QIs across sets as a minority were recommended by ≥3 societies (12%; 9/75 QIs). The most common QIs included postdischarge follow-up (73%, 8/11 societies), specific pharmacotherapy (64%, 7/11 societies), patient education (45%, 5/11 societies), assessment of left ventricular ejection fraction (45%, 5/11 societies), 30-day readmission rate (45%, 5/11 societies), cardiac rehabilitation (36%, 4/11 societies), and multidisciplinary management (27%, 3/11 societies).

CONCLUSIONS:
There was little agreement on defining high-quality care and limited agreement on measures including postdischarge follow-up, specific pharmacotherapies, patient education, assessment of left ventricular ejection fraction, 30-day readmission, cardiac rehabilitation, and multidisciplinary management. These measures may define high-quality care and highlight opportunities to improve the quality of care for adults hospitalized for heart failure.