bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of the microhaplotype markers in kinship analysis
Authors: Zhu Jing , Chen Peng , Qu Shengqiu , Wang Yinji , Jian Hui , Cao Shuqiang , Liu Yuqing , Zhang Ranran , Lv Meili , Liang Weibo , Zhang Lin 
Published: December 2018

AbstractMicrohaplotype markers are emerging forensic genetic markers, which may supplement existing markers. Consisting of two to four SNPs with an extent of <200 bp, microhaplotype can be genotyped through massively parallel sequencing technology. Articles that have been published suggested that microhaplotype markers have good application prospect in forensics. Multiallelic haplotype loci are potentially important in certain forensic works, as the stutter and high mutation rate of short tandem repeats and the low polymorphism of single nucleotide polymorphisms may limit the power of these two kind of regular markers. In this study, we explored the potential of 11 new microhaplotype loci in kinship analysis. The results suggested that these loci have relatively high polymorphic information in different populations worldwide and relatively high system effectiveness in the kinship analysis. Microhaplotypes have potential for forensic kinshipg analysis.
Microfluidic based impedance biosensor for pathogens detection in food products
Authors: Abdullah Amjed , Dastider Shibajyoti Ghosh , Jasim Ibrahem , Shen Zhenyu , Yuksek Nuh , Zhang Shuping , Dweik Majed , Almasri Mahmoud 
Published: December 2018

AbstractA MEMS‐based impedance biosensor was designed, fabricated, and tested to effectively detect the presence of bacterial cells including E. coli O157:H7 and Salmonella typhimurium in raw chicken products using detection region made of multiple interdigitated electrode arrays. A positive dielectrophoresis based focusing electrode was used in order to focus and concentrate the bacterial cells at the centerline of the fluidic microchannel and direct them toward the detection microchannel. The biosensor was fabricated using surface micromachining technology on a glass substrate. The results demonstrate that the device can detect Salmonella with concentrations as low as 10 cells/mL in less than 1 h. The device sensitivity was improved by the addition of the focusing electrodes, which increased the signal response by a factor between 6 and 18 times higher than without the use of the focusing electrodes. The biosensor is selective and can detect other types of pathogen by changing the type of the antibody immobilized on the detection electrodes. The device was able to differentiate live from dead bacteria.
Suitability of anodic stripping voltammetry for routine analysis of venous blood from raptors
Authors: González Fernando , Camacho María , Tiburón Natalia Pastor , Peña Manuel Zumbado , Rueda Luis Revuelta , Luzardo Octavio P. 
Published: December 2018

Abstract

Lead (Pb) poisoning is a significant threat faced by raptors. Hence, rapid Pb diagnosis has become a priority during the admission of raptors in wildlife recovery centers, and bench-top analyzers, such as LeadCare II ®, are routinely employed for this purpose. However, this device has been designed for conducting analyses of human blood Pb levels (BLLs), and the validity of this methodology for whole blood from raptors has, to date, rarely been assessed. In addition, a recent recall by the US Food and Drug Administration has recommended discontinuing the use of this analyzer for human venous blood because it may underestimate the BLL. We evaluated the precision of BLL measurements taken with LeadCare II by comparing them with those obtained with inductively coupled plasma mass spectrometry (ICP-MS). Our sample contained venous blood from 105 raptors belonging to 4 species. The results showed a good correlation between the 2 techniques (Spearman's r = 0.927, p &lt; 0.0001). The mean BLL with ICP-MS was 19.6 μg/dL; it was found to be 18.7 μg/dL with LeadCare II. A Bland-Altman analysis indicated that the bias between the mean differences was only 0.5 μg/dL, but it had a high standard deviation of bias (5.7 μg/dL) and 95% limits of agreement from –10.75 to 11.74 μg/dL. The present results indicated that LeadCare II has an overall sensitivity of 71.8% and a positive predictive value of 76.3%. The specificity of LeadCare II for detecting animals with low BLL (&lt;3.4 μg/dL) was 96.4%, and the negative predictive value (the probability that a value below the limit of detection of LeadCare II has a true correspondence with the actual value) was 100%. The present results indicated that, although LeadCare II might be imperfect in the estimation of BLLs in raptors, it performs reasonably well and might be employed in the clinical setting to assess patients potentially suffering from Pb poisoning. Environ Toxicol Chem 2019;38:737–747. © 2018 SETAC
Abstract
Use of LeadCare II in analyzing raptor venous blood.
Extended hybrid directional DRX with auxiliary active cycles for light traffic in 5G networks
Authors: Philip Ninu Rachel , Malarkodi B. 
Published: December 2018

AbstractIn recent years, energy efficiency has become a major cause of concern in mobile networks. The overwhelming applications and services provided by the evolving fifth‐generation (5G) technologies consume a substantial amount of energy, especially at the user equipment (UE). The 3rd Generation Partnership Project has proposed discontinuous reception (DRX) as a power saving technique for the UE. The DRX protocol has been used by different generation mobile standards. In this paper, the performance of hybrid directional DRX (HD‐DRX) for 5G communication with beam searching is improved upon by augmenting intermediate single packet active states in the short and long sleep cycles for light traffic in 5G networks. The supplementary active states save power by allowing the UE to receive packets within the sleep periods up to a certain threshold without transitioning to the active state. The short bursts of packets in the light traffic are received directly through the intermediate active states without beam searching. Once the given threshold exceeds, the system functions as normal HD‐DRX for the heavy traffic without the intermediate states. The proposed model is analyzed as a semi‐Markov process with the bursty packet data traffic model. The numerical results of the proposed model show 3.1% improvement in power saving and reduction in average delay by around 2.5 s compared to the HD‐DRX. Further studies can help deep dive into operationalizing this on a mass scale.
The syncollisional granitoid magmatism and crust growth during the West Qinling Orogeny, China: Insights from the Jiaochangba pluton
Authors: Kong Juanjuan , Niu Yaoling , Duan Meng , Shao Fengli , Xiao Yuanyuan , Zhang Yu , Guo Pengyuan , Sun Pu , Gong Hongmei 
Published: December 2018

The West Qinling Orogenic Belt (WQOB) is a major portion of the Qinling‐Dabie‐Sulu Orogen and holds essential information for understanding the protracted evolution of the north‐eastern branch of the Paleo‐Tethys in East Asia. In this study, we report our petrological, geochemical, and geochronological study on the five Triassic granitoid plutons of West Qinling with emphasis on the poorly studied Jiaochangba pluton with zircon U–Pb ages of 217.5 ± 1.6 Ma and 215.2 ± 1.2 Ma. The new data and the existing data on the other four plutons support the view that the West Qinling granitoids represent a magmatic response to the continental collision of the Yangtze Block (YB) with the North China Craton (NCC) in the Triassic. Like the other four plutons, the Jiaochangba pluton shows strong light rare earth element (REE) enrichment and weak heavy REE depletion ([La/Sm]N ≈ 7.14 ± 1.89; [Sm/Yb]N ≈ 4.63 ± 1.85) with varying negative Eu anomalies (Eu/Eu* ≈ 0.65 ± 0.20). In the N‐MORB normalized diagram, all the samples show relative enrichment in Rb, Pb, U, and K with negative Nb, Ta, P, and Ti anomalies, resembling those of the model continental crust. The Jiaochangba pluton has relatively lower (87Sr/86Sr)i(0.7062 to 0.7081), higher εNd(t) (−6.91 to −2.09), and εHf(t) (−5.57 to −0.14) than mature continental crust, which are consistent with their source being dominated by lower crust with significant mantle contributions. Mantle‐derived melt, which formed from partial melting of mantle wedge peridotite facilitated by dehydration of the subducted/subducting Mianlue ocean crust, provide the required heat for the crustal melting while also contributing to the compositions of these granitoids. Evolution of such parental magmas in open system crustal magma chambers with continued evolution/replenishment and crustal contamination and assimilation give rise to the observed petrological and geochemical characteristics of these granitoids.
Role of magma mixing in generating of the Gheshlagh–Aftabrow intrusions, SW Buin Zahra, Iran: Evidence for a juvenile origin from geochemical and Sr–Nd isotopic data
Authors: Kazemi Kazem , Kananian Ali , Xiao Yilin , Sarjoughian Fatemeh 
Published: December 2018

In this paper, we investigate host granodiorites and associated mafic microgranular enclaves (MMEs), including gabbroic to quartz diorite enclaves from the Middle Eocene Gheshlagh–Aftabrow granitoid, to assess magma mixing and crust–mantle interaction in the Urumieh–Dokhtar magmatic arc. The host granodiorites yield zircon U–Pb ages of ca. 40 Ma. Geochemical and bulk rock Nd–Sr isotopic modelling, together with the widespread occurrence of mafic microgranular enclaves with ellipsoidal and spherical shapes and chilled margins, K‐feldspar and plagioclase megacrysts in the enclaves, and the presence of acicular apatite, ocellar quartz, plagioclase with oscillatory zoning and resorption surfaces, suggests that the mafic microgranular enclaves are globules of a more mafic magma derived from the mantle that was injected into and mixed/mingled with the magma derived from partial melting of a lower crust. Geochemically, the granitoid rocks are high‐K calc‐alkaline and metaluminous (A/CNK = 0.87–0.92) and belong to I‐type suite. These granitoid host rocks have much higher SiO2 (65.4–67.3 wt%) and relatively low Fe2O3 (1.8–2.1 wt%) and MgO (1–1.58 wt%), so compared with the host rocks, the mafic microgranular enclaves have much lower SiO2 contents (50.5–55 wt%), considerably higher Fe2O3 (3.4–4.2 wt%) and MgO (3.9–4.8 wt%). Both the host intrusion and enclaves are enriched in LREE relative to HREEs (LREE/HREE = 3.1–4.1; (La/Yb)N = 2.1–4.1) and show slightly negative Eu anomalies (δEu = 0.75–0.90) and nearly flat HREE patterns ((Gd/Yb)N = 0.86–1.26). Analyses of Sr–Nd isotopes in the host granodiorites and associated enclaves show (87Sr/86Sr)i host = 0.705070–0.705174, εNd(t) host = 0.21–2.31, TDM1 host = 782–983 Ma, (87Sr/86Sr)i enclave = 0.704997–0.705170, εNd(t) enclave = 2.15–2.17, and TDM1 enclave = 874–980 Ma, indicating of juvenile nature for these granites. This characteristic together with similar trace‐element compositions indicates intense magma mixing and a high degree of geochemical equilibration between the host granodiorites and their enclaves.A binary mixing model based on Sr–Nd isotopic compositions of the GAG indicates that rocks were mostly generated by mixing of 7–12% melts produced by partial melting of meta‐igneous old lower crust rocks with 88–93% contribution of mantle‐derived juvenile basaltic magmas.
Comparison of homofugality among alkyl groups attached to tin atom
Authors: Kamimura Akio , Yoshinaga Tatsuro , Miyazaki Koichiro , Kawamoto Takuji 
Published: December 2018

AbstractDibutylmethyltin hydride and tert‐butyldibutyltin hydride were employed to examine radical cascade reaction of aza‐1,6‐enyne for the preliminary estimation of homofugality between alkyl groups by product distribution analysis. Use of either dibutylmethyltin hydride or tert‐butyldibutyltin hydride preferentially produced butylmethylstannolane, in which butyl group was mainly substituted by vinyl radical.
PI3‐kinase pathway biomarkers in oral cancer and tumor immune cells
Authors: Ibrahim Mohammad Y. , Nunez Maria I. , Harun Nusrat , Lee J. Jack , El‐Naggar Adel K. , Ferrarotto Renata , Wistuba Ignacio , Myers Jeffrey , Glisson Bonnie S. , William William N. 
Published: December 2018

AbstractBackgroundThis study investigated the hypothesis that phosphoinositide 3‐kinase (PI3‐kinase) pathway dysregulation in either head and neck cancer cells and/or tumor infiltrating immune cells would influence outcomes of patients with surgically treated oral tongue squamous cell carcinomas (SCC).MethodsWe constructed tissue microarrays containing 123 oral tongue SCC samples and performed immunohistochemistry using antibodies against 7 PI3‐kinase pathway markers: phosphatase and tensin homolog (PTEN), Akt, p‐Akt, mammalian target of rapamycin (mTOR), phosphorylated‐mammalian target of rapamycin (p‐mTOR), survivin, and Ki‐67). Expression levels in cancer cells or tumor infiltrating immune cells were correlated with outcomes.ResultsHigher levels of PTEN expression in immune cells were significantly associated with improved recurrence‐free survival (heart rate (HR) = 0.45, 95% confidence interval (CI) 0.23‐0.90, P = .03), and overall survival (HR = 0.34, 95% CI 0.15‐0.76, P = .01) on univariate and multicovariate models.ConclusionsWe identified a novel, negative prognostic role of PI3‐kinase activation (as determined by PTEN loss) in oral SCC infiltrating immune cells. These findings could be relevant for clinical development of PI‐3 kinase inhibitors for this disease.
Initial experience with image‐guided surgical navigation in transoral surgery
Authors: Paydarfar Joseph A. , Wu Xiaotian , Halter Ryan J. 
Published: December 2018

AbstractBackgroundSurgical navigation using image guidance may improve the safety and efficacy of transoral surgery (TOS); however, preoperative imaging cannot be accurately registered to the intraoperative state due to deformations resulting from placement of the laryngoscope or retractor. This proof of concept study explores feasibility and registration accuracy of surgical navigation for TOS by utilizing intraoperative imaging.MethodsFour patients undergoing TOS were recruited. Suspension laryngoscopy was performed with a CT‐compatible laryngoscope. An intraoperative contrast enhanced CT scan was obtained and registered to fiducials placed on the neck, face, and laryngoscope.ResultsAll patients were successfully scanned and registered. Registration accuracy within the pharynx and larynx was 1 mm or less. Target registration was confirmed by localizing endoscopic and surface structures to the CT images. Successful tracking was performed in all 4 patients.ConclusionFor surgical navigation during TOS, although a high level of registration accuracy can be achieved by utilizing intraoperative imaging, significant limitations of the existing technology have been identified. These limitations, as well as areas for future investigation, are discussed.
Radiation therapy for patients with newly diagnosed metastatic head and neck squamous cell carcinoma
Authors: Kabarriti Rafi , Baliga Sujith , Ohri Nitin , Guha Chandan , Kalnicki Shalom , Garg Madhur K. 
Published: December 2018

AbstractBackgroundTo assess the association between radiation therapy (RT) use and overall survival (OS) for patients with metastatic head and neck squamous cell carcinomas (HNSCC).MethodsWe analyzed the National Cancer Database to identify patients with newly diagnosed metastatic HNSCC from 2004 to 2013 who were treated with up‐front chemotherapy. Associations between the use of RT and OS were evaluated.ResultsWe identified 3516 patients with metastatic HNSCC who were treated with chemotherapy; 2288 (65%) were also treated with RT. The addition of RT to chemotherapy was associated with prolonged survival (median: 13.6 vs 11.3 months, log‐rank P < .001). After adjusting for other prognostic factors in a multivariable Cox model, propensity score matching and landmark analyses limited to patients who survived at least 3, 6, and 12 months after diagnosis, the use of RT remained associated with prolonged survival.ConclusionThe addition of RT to chemotherapy in the up‐front management of metastatic HNSCC was associated with prolonged OS in this study.
Observational and predictive uncertainties for multiple variables in a spatially distributed hydrological model
Authors: Ehlers Lennart Benjamin , Sonnenborg Torben Obel , Refsgaard Jens Christian 
Published: December 2018

AbstractIn this study, uncertainty in model input data (precipitation) and parameters is propagated through a physically based, spatially distributed hydrological model based on the MIKE SHE code. Precipitation uncertainty is accounted for using an ensemble of daily rainfall fields that incorporate four different sources of uncertainty, whereas parameter uncertainty is considered using Latin hypercube sampling. Model predictive uncertainty is assessed for multiple simulated hydrological variables (discharge, groundwater head, evapotranspiration, and soil moisture). Utilizing an extensive set of observational data, effective observational uncertainties for each hydrological variable are assessed. Considering not only model predictive uncertainty but also effective observational uncertainty leads to a notable increase in the number of instances, for which model simulation and observations are in good agreement (e.g., 47% vs. 91% for discharge and 0% vs. 98% for soil moisture). Effective observational uncertainty is in several cases larger than model predictive uncertainty. We conclude that the use of precipitation uncertainty with a realistic spatio‐temporal correlation structure, analyses of multiple variables with different spatial support, and the consideration of observational uncertainty are crucial for adequately evaluating the performance of physically based, spatially distributed hydrological models.
Rs1894720 polymorphism in MIAT increased susceptibility to age‐related hearing loss by modulating the activation of miR‐29b/SIRT1/PGC‐1α signaling
Authors: Hao Shaojuan , Wang Le , Zhao Kun , Zhu Xiaodan , Ye Fanglei 
Published: December 2018

AbstractBackgroundMIAT may be implicated in the pathogenesis of age‐related hearing loss (AHL). This study aimed to clarify the effect of a MIAT signaling pathway on the risk of AHL.MethodsTerminal deoxynucleotidyl transferase dUTP nick‐end labeling assay, auditory brainstem response (ABR) and quantitative hair cell counts were used to compare the hearing functions in different groups of mice. 5,5,6,6‐Tetrachloro‐1,1,3,3‐tetraethylbenzimidazolylcarbocyanine iodide (JC‐1) dye method was used to establish the potential association between mitochondrial dysfunction and aging. Real‐time polymerase chain reaction, Western blot analysis, computational analysis, and luciferase assay were conducted to establish a myocardial infarction associated transcript (MIAT) signaling pathway, whose role in the pathogenesis of AHL was further validated by 3‐[4,5‐dimethylthiazol‐2‐yl]‐2,5 diphenyl tetrazolium bromide (MTT) assay and flow cytometry.ResultsAged C57BL/6 mice were associated with a more severe level of hair cell loss, while exhibiting a higher ABR threshold at various frequencies as well as a lower percentage of inner/outer hair cells. A reduced mitochondrial membrane potential in the cochleae of aged C57BL/6 mice indicated the presence of mitochondrial dysfunction in these mice. Relative expression of MIAT, Sirtuin1 (SIRT1), and peroxisome proliferator‐activated receptor γ coactivator 1α (PGC‐1α) was downregulated in aged mice, with microRNA‐29b (miR‐29b) being highly expressed. Also, MIAT binds to miR‐29b, an inhibitor of SIRT1 expression. The regulatory relationship among MIAT, miR‐29b, and SIRT1 was further validated by comparing the differentiated expression of these factors in cells treated with phosphate‐buffered saline + H2O2, a negative control + H2O2, MIAT + H2O2, or H2O2 + anti‐miR‐29b.ConclusionMIAT could elevate the expression of SIRT1/PGC‐1α via downregulating miR‐29b. And the downregulated SIRT/PGC‐1α increased the incidence of AHL via promoting the apoptosis of cochlear hair cells.
Engineered zinc‐finger nuclease to generate site‐directed modification in the KLF1 gene for fetal hemoglobin induction
Authors: Shariati Laleh , Modarressi Mohammad Hossein , Tabatabaiefar Mohammad Amin , Kouhpayeh Shirin , Hejazi Zahra , Shahbazi Mansoureh , Sabzehei Faezeh , Salehi Mansoor , Khanahmad Hossein 
Published: December 2018

AbstractElevation of hemoglobin F (HbF) ameliorates symptoms of β‐thalassemia, as a common autosomal recessive disorder. In this study, the ability of an engineered zinc‐finger nuclease (ZFN) system was assesed to disrupt the KLF1 gene to inhibit the γ to β hemoglobin switching in K562 cells. This study was performed using a second generation integration‐deficient lentiviral vector assigned to transient gene targeting. The sequences coding for zinc finger protein arrays were designed and subcloned in TDH plus as a transfer vector. Transduction of K562 cells was performed with the integrase minus lentivirus containing ZFN. The indel percentage of the transducted cells with lentivirus containing ZFN was about 29%. Differentiation of K562 cell line into erythroid cell lineage was induced with cisplatin concentration of 15 µg/mL. After differentiation, γ‐globin and HbF expression were evaluated using real‐time reverse‐transcription polymerase chain reaction and hemoglobin electrophoresis methods. The levels of γ‐globin messenger RNA were nine‐fold higher in the ZFN treated cells compared with untreated cells 5 days after differentiation. Hemoglobin electrophoresis method showed the same results for HbF level measurement. Application of the ZFN tool to induce KLF1 gene mutation in adult erythroid progenitors might be a candidate to stimulate HbF expression in β‐thalassemia patients.
Association study between two polymorphisms of tumor necrosis factor ligand superfamily member 15 (TNFSF15) gene and ulcerative colitis in south‐west of Iran
Authors: Taheri Marzieh , Ghandil Pegah , Hashemi Seyyed Jalal , Ghafourian Mehri , Masjedi Zadeh Abdol Rahim , Ghadiri Ata Allah 
Published: December 2018

AbstractBackground and aimsUlcerative colitis (UC) is the most prevalent clinical manifestation of the inflammatory bowel disease (IBD). Several candidate genes have been suggested to be involved in the genetic susceptibility or resistance in the development of UC. Among them, tumor necrosis factor ligand superfamily member 15 (TNFSF15) have been reported in association with IBD in several studies. The aim of this study was to investigate the association of TNFSF15 gene polymorphisms located in the promoter region, including rs6478108 (G/A −9706) and rs3810936 (G/A −15524) in Iranian patients with UC.MethodsIn this way, the two single nucleotide polymorphisms were studied in 115 patients with UC and 115 healthy controls with the same ethnic group from south‐west of Iran. The genomic DNA of samples was genotyped using TaqMan Real‐time PCR assay. This case‐control study was conducted at the Department of Immunology, Jundishapur University of Medical Sciences, Ahvaz, Iran.Results and conclusionOur results did not confirm the formerly reported association of the studied polymorphisms with UC disease in comparison with healthy controls, neither with the type of the clinical forms of Colitis in the studied Iranian population. Comparing the genotype frequency of single nucleotide polymorphism (SNP) rs6478108, wild‐type homozygous and heterozygote and mutant homozygote were 33%, 55.7%, and 11.3% in cases vs. 34.8%, 50.4%, and 14.8% in the controls (P = 0.6). The genotype frequency of SNP rs3810936 were 20.9%, 40.9%, and 38.2% in the cases compared to 18.3%, 44.3%, and 37.4% in controls, which was not significant (
P = 0.8). As multiple ethnic groups reside in all around the country, further studies using different ethnicities and/or larger sample size are required to clarify the role of these polymorphisms in the genetic susceptibility of UC in Iranian populations.