bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dorsal ruffles enhance activation of Akt by growth factors
Authors: Yoshida Sei , Pacitto Regina , Sesi Catherine , Kotula Leszek , Swanson Joel A. 
Published: October 2018

ABSTRACT
In fibroblasts, platelet-derived growth factor (PDGF) and epidermal growth factor (EGF) stimulate the formation of actin-rich, circular dorsal ruffles (CDRs) and phosphatidylinositol 3-kinase (PI3K)-dependent phosphorylation of Akt. To test the hypothesis that CDRs increase synthesis of phosphorylated Akt1 (pAkt), we analyzed the contributions of CDRs to Akt phosphorylation in response to PDGF and EGF. CDRs appeared within several minutes of growth factor addition, coincident with a peak of pAkt. Microtubule depolymerization with nocodazole blocked CDR formation and inhibited phosphorylation of Akt in response to EGF but not PDGF. Quantitative immunofluorescence showed increased concentrations of Akt, pAkt and phosphatidylinositol (3,4,5)-trisphosphate (PIP3), the phosphoinositide product of PI3K that activates Akt, concentrated in CDRs and ruffles. EGF stimulated lower maximal levels of pAkt than did PDGF, which suggests that Akt phosphorylation requires amplification in CDRs only when PI3K activities are low. Accordingly, stimulation with low concentrations of PDGF elicited lower levels of Akt phosphorylation, which, like responses to EGF, were inhibited by nocodazole. These results indicate that when receptor signaling generates low levels of PI3K activity, CDRs facilitate local amplification of PI3K and phosphorylation of Akt.
This article has an associated First Person interview with the first author of the paper.
GCN5L1 interacts with αTAT1 and RanBP2 to regulate hepatic α-tubulin acetylation and lysosome trafficking
Authors: Wu Kaiyuan , Wang Lingdi , Chen Yong , Pirooznia Mehdi , Singh Komudi , Wälde Sarah , Kehlenbach Ralph H. , Scott Iain , Gucek Marjan , Sack Michael N. 
Published: October 2018

ABSTRACT
Although GCN5L1 (also known as BLOC1S1) facilitates mitochondrial protein acetylation and controls endosomal-lysosomal trafficking, the mechanisms underpinning these disparate effects are unclear. As microtubule acetylation modulates endosome-lysosome trafficking, we reasoned that exploring the role of GCN5L1 in this biology may enhance our understanding of GCN5L1-mediated protein acetylation. We show that α-tubulin acetylation is reduced in GCN5L1-knockout hepatocytes and restored by GCN5L1 reconstitution. Furthermore, GCN5L1 binds to the α-tubulin acetyltransferase αTAT1, and GCN5L1-mediated α-tubulin acetylation is dependent on αTAT1. Given that cytosolic GCN5L1 has been identified as a component of numerous multiprotein complexes, we explored whether novel interacting partners contribute to this regulation. We identify RanBP2 as a novel interacting partner of GCN5L1 and αTAT1. Genetic silencing of RanBP2 phenocopies GCN5L1 depletion by reducing α-tubulin acetylation, and we find that RanBP2 possesses a tubulin-binding domain, which recruits GCN5L1 to α-tubulin. Finally, we find that genetic depletion of GCN5L1 promotes perinuclear lysosome accumulation and histone deacetylase inhibition partially restores lysosomal positioning. We conclude that the interactions of GCN5L1, RanBP2 and αTAT1 function in concert to control α-tubulin acetylation and may contribute towards the regulation of cellular lysosome positioning.
This article has an associated First Person interview with the first author of the paper.
Constitutive P2Y2 receptor activity regulates basal lipolysis in human adipocytes
Authors: Ali Seema B. , Turner Jeremy J. O. , Fountain Samuel J. 
Published: October 2018

ABSTRACT
White adipocytes are key regulators of metabolic homeostasis, which release stored energy as free fatty acids via lipolysis. Adipocytes possess both basal and stimulated lipolytic capacity, but limited information exists regarding the molecular mechanisms that regulate basal lipolysis. Here, we describe a mechanism whereby autocrine purinergic signalling and constitutive P2Y2 receptor activation suppresses basal lipolysis in primary human in vitro-differentiated adipocytes. We found that human adipocytes possess cytoplasmic Ca2+ tone due to ATP secretion and constitutive P2Y2 receptor activation. Pharmacological antagonism or knockdown of P2Y2 receptors increases intracellular cAMP levels and enhances basal lipolysis. P2Y2 receptor antagonism works synergistically with phosphodiesterase inhibitors in elevating basal lipolysis, but is dependent upon adenylate cyclase activity. Mechanistically, we suggest that the increased Ca2+ tone exerts an anti-lipolytic effect by suppression of Ca2+-sensitive adenylate cyclase isoforms. We also observed that acute enhancement of basal lipolysis following P2Y2 receptor antagonism alters the profile of secreted adipokines leading to longer-term adaptive decreases in basal lipolysis. Our findings demonstrate that basal lipolysis and adipokine secretion are controlled by autocrine purinergic signalling in human adipocytes.
Establishing an international laboratory network for neglected tropical diseases: Understanding existing capacity in five WHO regions
Authors: Dean Laura , Njelesani Janet , Mulamba Charles , Dacombe Russell , Mbabazi Pamela S. , Bates Imelda 
Published: October 2018

Background. Limited laboratory capacity is a significant bottleneck in meeting global targets for the control and elimination of neglected tropical diseases (NTD). Laboratories are essential for providing clinical data and monitoring data about the status and changes in NTD prevalence, and for detecting early drug resistance. Currently NTD laboratory networks are informal and specialist laboratory expertise is not well publicised, making it difficult to share global expertise and provide training, supervision, and quality assurance for NTD diagnosis and research. This study aimed to identify laboratories within five World Health Organisation regions (South-East Asia, Eastern Mediterranean, Americas, Western Pacific and Europe) that provide NTD services and could be regarded as national or regional reference laboratories, and to conduct a survey to document their networks and capacity to support NTD programmes. Methods. Potential NTD reference laboratories were identified through systematic searches, snowball sampling and key informants. Results. Thirty-two laboratories responded to the survey. The laboratories covered 25 different NTDs and their main regional and national roles were to provide technical support and training, research, test validation and standard setting. Two thirds of the laboratories were based in academic institutions and almost half had less than 11 staff. Although greater than 90 per cent of the laboratories had adequate technical skills to function as an NTD reference laboratory, almost all laboratories lacked systems for external verification that their results met international standards. Conclusions. This study highlights that although many laboratories believed they could act as a reference laboratory, only a few had all the characteristics required to fulfil this role as they fell short in the standard and quality assurance of laboratory processes. Networks of high quality laboratories are essential for the control and elimination of disease and this study presents a critical first step in the development of such networks for NTDs.
An in vitro assessment of bacterial transfer by products used in debridement
Authors: Rippon Mark G. , Rogers Alan A. , Sellars Laura , Purcell Liam E. J. , Westgate Samantha 
Published: October 2018

Objective: The aim of this in vitro study was to investigate the transfer of viable Pseudomonas aeruginosa biofilm microorganisms following treatment with debridement tools. Method: The level of viable biofilm microorganisms transferred by debridement tools was compared following treatment that reflected the clinical practice of each product. Results: A significant level of microorganism transfer was seen in response to the mechanical debridement tool. Minimal transfer of microorganisms was seen when in vitro-established biofilms were treated with hydroresponsive wound dressing + polyhexamethylene biguanide (HRWD+PHMB, HydroClean plus). Less Pseudomonas aeruginosa was recovered from explants exposed to dressings compared with those exposed to debridement tools suggesting that there was less transfer of bacteria by dressings. Conclusion: The reduced transfer of viable microorganisms by HRWD+PHMB may be the result of significant binding and retention of microbes by the superabsorbent polymer within the dressing, together with enhanced sequestered bacterial killing within the dressing by polymer-bound PHMB. The high levels of microbial transfer/transmission seen for debridement tools suggests that, in the clinical setting, a significant level of bacterial spread over the wound surface and/or surrounding skin by these cleansing tools is likely.
A Scientific Note of Housekeeping Genes for the Primitively Eusocial bee Euglossa viridissima Friese (Apidae: Euglossini)
Authors: Boff Samuel , Friedel Anna , Miertsch Anja , Quezada-Euàn J. Javier , Paxton Robert J , Lattorff H. Michael G 
Published: October 2018

Studies on the expression of genes in different contexts are essential to our understanding of the functioning of organisms and their adaptations to the environment. Gene expression studies require steps of normalization, which are done using the stable expression pattern of reference genes. For many different eusocial bees reference genes have been discovered, but not for the primitively eusocial euglossine bees.We used available genomic resources of euglossine species and the gene information of Apis melliferato develop a set of reference genes for the primitive eusocial bee Euglossaviridissima. We tested nine genes in distinct developmental stages three different algorithms to infer the stability of gene expression. The Tata binding protein(Tbp) and 14-3-3epsilon were the most stable genes across all different stages. The strongest deviation in gene expression pattern occurred in pupae, which require a different set of genes for normalizing gene expression.
Afrofuturism: The World of Black Sci-Fi and Fantasy Culture
Authors: Kam Shaun Ting Sheng 
Published: October 2018

Abstract
Afrofuturism: The World of Black Sci-Fi and Fantasy Culture, Ytasha L. Womack, (2013)
Chicago: Chicago Review, 224pp., ISBN: 9781613747964 (pbk), $16.95
DVD/Blu-ray
Authors: Fulton Gabrielle , Lowe Kaitlin , Johnson Matthew , Soman Shaun , Thompson Blake , Saller Kelsey , Linden Tyler , Bankowski Lizzie , Smallwood Kimberly Mariah , Staub Chamberlain , Triplett Steph 
Published: October 2018

Abstract
The Umbrellas of Cherbourg (1964)
France Director Jacques Demy Runtime 91 minutes
Blu-ray USA, 2017 Distributed by The Criterion Collection (region A/1)
L’Argent (1983)
France Director Robert Bresson Runtime 84 minutes
Blu-ray USA, 2017 Distributed by The Criterion Collection (region A/1)
A Cavalcade of Perversions
Multiple Maniacs (1970)
USA Director John Waters Runtime 96 minutes
Blu-ray USA, 2017 Distributed by The Criterion Collection (region A/1)
Women on the Verge of a Nervous Breakdown (1988)
Spain Director Pedro Almodóvar Runtime 89 minutes
Blu-ray USA, 2017 Distributed by The Criterion Collection (region A/1)
Roberto Rossellini’s War Trilogy
Rome Open City (1945) Paisan (1946) Germany Year Zero (1948)
Italy Director Roberto Rossellini Runtime 103 minutes; 126 minutes; 73 minutes
Blu-ray USA, 2017 Distributed by The Criterion Collection (region A/1)
Paris Belongs to Us in 2 Frames
Paris Belongs to Us (1961)
France Director Jacques Rivette Runtime 141 minutes
Blu-ray USA, 2016 Produced and Distributed by The Criterion Collection (region A/1)
Judex in 4 Frames
Judex (1963)
France Director Georges Franju Runtime 97 minutes
Dual Format Blu-ray/DVD USA, 2014 Produced and Distributed by The Criterion Collection (region A/1)
Characterization of Pinus nigra var. laricio [Maire] bark extracts at the analytical and pilot scale
Authors: Bianchi Sauro , Zikeli Florian , Vinciguerra Vittorio , Kroslakova Ivana , Mayer Ingo , Pichelin Frédéric , Matteucci Giorgio 
Published: October 2018

Abstract

Pinus nigra var. laricio bark and its hot-water extracts (HWE) obtained at an analytical and pilot plant scale have been characterized in terms of phenolic extractives, condensed tannins (CTs), carbohydrates and inorganic compounds. Analytical extractions with aqueous acetone were also performed for comparison with HWE. The bark contains 35.5 g kg−1 CT, and two-thirds of it could be extracted. Analytical HWE at 75°C led to a total yield of 56.4 g kg−1. The extracts are mainly composed of phenolic compounds (50.7%) and pectins (19.7%). CTs amount to 17.9% of the extracts and are procyanidins with a mean degree of polymerization (DP) of about 9. Non-tannin phenolic oligomers also occurred in the extracts, which could be identified by pyrolysis gas chromatography mass spectrometry (Py-GC/MS) as lignin fragments. Matrix-assisted laser desorption/ionization-time-of-flight-mass spectrometry (MALDI-TOF MS) revealed that the CT is a flavanol derivative in methylated form. Further characterization and tailoring of the HWE properties is needed in the context of their specific application.
Influence of length on acoustic time-of-flight (ToF) measurement in built-in structures of Norway spruce timber
Authors: Arriaga Francisco , Montón Joaquín , Bobadilla Ignacio , Llana Daniel F. 
Published: October 2018

Abstract
Time-of-flight (ToF) measurements were conducted on twelve 76×226-mm2 in cross-section, 4.52-m long specimens of Norway spruce timber pieces from a dismantled 19th-century building in Barcelona (Catalonia, Spain). Two commercially available acoustic devices were used: the USLab ultrasound device with conical 22 and 45 kHz sensors and the MicroSecond Timer (MST) stress wave device with spike sensors. ToF were obtained for the full-length (4.52 m) specimens in an end-to-end arrangement and for lengths of 0.5, 1, 1.5, 2, 3 and 4 m on the same surface and on opposite surfaces. The differences between the velocities obtained from end-to-end and semi-direct measurements were less than 4.5%. Apparent velocity dependence on length was observed in short-distance measurements. This could be corrected by determining a time lag (tL) from a linear regression between the ToF and the distance. Estimation of the modulus of elasticity (MOE) from the dynamic MOE (MOEdyn) is acceptable (r2=0.45–0.69) depending on the measurement procedure, and the best results for in situ timber are obtained in edge surface velocity. Although modulus of rupture (MOR) estimation from MOEdyn improves slightly when knottiness is included, it has low predictive capacity.
Relationship Between Toe Muscular Strength and the Ability to Change Direction in Athletes
Authors: Yuasa Yasuhiro , Kurihara Toshiyuki , Isaka Tadao 
Published: October 2018

Abstract
This study aimed to investigate the relationship between toe muscular strength and the ability to change direction in athletes. Seventeen collegiate American-football players participated in the study (age 19.9 ± 0.9 years, competition experience 7.3 ± 1.7 years). Two types of measurements were performed to evaluate toe muscular strength: toe flexor strength with the metatarsophalangeal joint in the planter flexed position and toe-pushing force with the metatarsophalangeal joint in the dorsiflexed position. The ability to change direction was evaluated using the pro-agility and 3-cone tests and change of direction deficits, calculated by subtracting the sprint times from the pro-agility and 3-cone times. There were significant correlations between toe-pushing force and the results of the pro-agility and 3-cone tests, but no significant correlations between toe flexor strength and the pro-agility and 3-cone tests. Neither toe-pushing force nor toe flexor strength was significantly correlated with the sprint test results. Furthermore, toe-pushing force was significantly correlated with the 3-cone test deficit, but toe flexor strength was not. The ability to change direction is more strongly affected by toe muscular strength (measured as toe-pushing force) with the metatarsophalangeal joint in the dorsiflexed angle than by toe muscular strength (measured as toe flexor strength) with the metatarsophalangeal joint in the plantar flexed angle. Our results suggest that athletes can improve their ability to change direction with toe muscular strength training with the metatarsophalangeal joint in the dorsiflexed position.
Training‐Induced Variations in Haematological and Biochemical Variables in Adolescent Athletes of Arab Origin Throughout an Entire Athletic Season
Authors: Varamenti Evdokia , Nikolovski Zoran , Elgingo Mohamed I. , Jamurtas Athanasios Z. , Cardinale Marco 
Published: October 2018

Abstract
The purpose of this study was to observe and report variations in several haematological and biochemical markers throughout an entire athletic season in a large cohort of adolescent athletes of Arab origin. Blood samples were collected from 72 adolescent male athletes at 4 selected time points during their training season. Results expressed in relation to plasma volume were corrected accordingly and significant variations in several variables emerged. Initial uncorrected haematological results revealed that haematocrit (Hct) and mean cell volume (MCV) concentrations noticeably increased at the competitive period (T3) and before the start of the following preseason (T4), whereas reticulocytes equivalent (Ret-He) only rose at T4 phase (p < 0.01). Conversely, corrected red blood cells (RBC), haemoglobin (Hb) and mean cell haemoglobin concentration (MCHC) progressively decreased over the year (p < 0.001). From the electrolytes panel, sodium and chloride considerably reduced at the peak of the training period (T2) to the start of the next preseason (T4), while a significant fall in potassium was mainly observed during the competitive period (T3) (p < 0.001). Coaches and sport scientists could use the results of this study to evaluate typical variations of each age group in order to diagnose potential adverse effects of high training loads, assist in the design of training programs and/or clinical interventions that will safeguard athletes’ health, and consider the important role of plasma volume for the interpretation of results.
Physiological and Psychological Adaptations of Trained Cyclists to Spring Cycling Camps
Authors: Dionne Jean-François , Lajoie Claude , Gendron Philippe , Freiberger Eduardo , Trudeau François 
Published: October 2018

Abstract
The purpose of our study was to assess physiological adaptations and measure mood outcomes following a cycling training camp in competitive athletes. Fourteen competitive athletes (8 males, 6 females) performed 2 incremental tests to exhaustion before and after a training camp. Volume and intensity (load) of the training regimen were recorded. Submaximal and maximal metabolic data were analysed, as well as economy variables (gross mechanical efficiency and cycling economy). Skeletal muscle adaptations were assessed using near infrared spectroscopy (NIRS). For both genders (n = 14), peak power output, peak power output-W/kg ratio and peak power output-B[La] were significantly increased (p < 0.05) after the cycling training camp (p < 0.05). Significant increases occurred for gross mechanical efficiency measured at the lactate threshold (+4.9%) and at the same precamp lactate threshold power output (+2.9%). At the lactate threshold and Post Camp Lactate Threshold Power, cycling economy increased by 5.2 and 2.9%, respectively (p < 0.05). These power measurements were significantly correlated with individual fluctuations in deoxyhaemoglobin in the vastus lateralis for male cyclists only. Profile of Mood State questionnaire results showed that subcategories “Tension-Anxiety”, “Confusion”, “Fatigue” and “Total Global Score” significantly decreased after the training camp. Cycling training camps were associated with positive adaptations (increased cycling economy, gross mechanical efficiency and power output) as well as some mental benefits. This indicates that despite some significant physiological adaptations participants probably did not overreach during their CTC.
Successful and Unsuccessful Offensive Sequences Ending in a Shot in Professional and Elite Under-16 Basketball
Authors: Alsasua Roberto , Lapresa Daniel , Arana Javier , Teresa Anguera M. , Garzón Belén 
Published: October 2018

Abstract
Following observational methodology, we analyzed successful and unsuccessful offensive attacks by professional and elite under-16 (U16) basketball players in Spain using an adapted ad hoc observation instrument designed to study efficiency in basketball. We identified both similarities and differences between how players from both categories built their attacks. The synchronic statistical analysis based on frequency counts showed that shots were more efficient in professional basketball and that U16 basketball was less static and had a higher frequency of fast breaks. Diachronic analysis, which consisted of T-pattern detection using Theme software, allowed us to identify characteristic successful and unsuccessful offensive sequences in professional and elite U16 basketball. These results have practical implications as they can be used to design training drills and prepare for competitions in U16 and professional basketball.
Influence of the Varied Pitch Shape on Soccer Players Physiological Responses and Time-Motion Characteristics During Small-Sided Games
Authors: Casamichana David , Bradley Paul S , Castellano Julen 
Published: October 2018

Abstract
The aim of this study was to investigate the effect of pitch shape modifications on heart rate responses and time-motion characteristics in soccer players during 5-a-side small-sided games (SSGs). Players completed four different SSG dimensions: (1) short narrow pitch (SN; 40 × 25 m), (2) short wide pitch (SW; 66 × 25 m), (3) long narrow pitch (LN; 40 × 50 m), and (4) long wide pitch (LW; 66 × 50 m). Twenty amateur soccer players (age: 21 ± 5 yr; stature: 176.8 ± 1.9 cm; body mass: 72.7 ± 3.7 kg) were monitored using a heart rate monitor and a 10 Hz GPS device. Mean maximum heart rate (%HRmax), rating of perceived exertion (RPE), peak running speed, total distance covered (TD), distance covered in four speed categories, number of moderate and high accelerations (Ac), decelerations (Dc), changes of direction (COD) and player load were recorded. Increasing the pitch length had a greater effect compared to increasing the pitch width especially on RPE (3.8, 6.3, 4.9 and 6.6 AU to SN, LN, SW and LW, respectively) and time-motion characteristics such as TD (101, 127, 108 and 131 m·min-1 to SN, LN, SW and LW, respectively), peak speed (4.8, 6.1, 5.2 and 6.2 m·s-1 to SN, LN, SW and LW, respectively), and the number of accelerations, decelerations, and changes of direction. The data demonstrates that increasing the length rather than the width of 5-a-side SSG has a greater impact on players’ responses in terms of increasing workloads.