bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nanomaterial‐Mediated Modulation of TRPV1 Ion Channels for Biomedical Applications
Authors: Pei Peng , Du Yafei , Wang Jiong‐Wei , Liu Xiaogang 
Published: December 2024

AbstractTransient receptor potential vanilloid subtype 1 (TRPV1) is a nonselective cation channel involved in various physiological processes such as pain perception, thermoregulation, and inflammatory responses. Nanomaterials have emerged as precise tools to modulate TRPV1 activity, offering high spatiotemporal resolution and specificity. These nanomaterials act as transducers, responding to internal or external stimuli such as pH, light, electric, and magnetic fields to deliver modulatory agents like agonists, antagonists, heat, reactive species, and mechanical forces to TRPV1 channels. This strategy enables non‐invasive and targeted therapeutic interventions for diseases associated with TRPV1 dysfunction. In this review, recent advances are highlighted in nanomaterial‐mediated TRPV1 modulation and its biomedical applications. The TRPV1 structure and activation mechanisms, the integration of nanomaterials for effective TRPV1 modulation, and the required material properties are covered. Moreover, biomedical applications are discussed, including neurostimulation, neurological disorder therapies, cancer therapies, metabolic disease treatments, and cardiovascular disease interventions. Future research directions and challenges in this field are also proposed.
Arterial calcification in the heart–brain axis and cognitive performance over time
Authors: Streiber Anna M. , van den Beukel Tim C. , vom Hofe Ilse , Neitzel Julia , Vernooij Meike W. , Bos Daniel , Vinke Elisabeth J. 
Published: December 2024

AbstractBACKGROUNDWhile cross‐sectional studies suggest a link between arteriosclerosis and cognition, longitudinal research is lacking. We investigated how arteriosclerosis in the heart–brain axis is related to cognitive performance trajectories over time.METHODSWithin the population‐based Rotterdam Study, 2368 participants underwent noncontrast CT to quantify arterial calcification, a hallmark of arteriosclerosis, in five major arteries in the heart–brain axis. Following this scan, participants underwent repeated cognitive testing. The association between baseline calcification and longitudinal cognitive test performance was investigated using mixed models.RESULTSHigher calcification was associated with worse baseline cognitive performance across domains (e.g., β for global cognition per 10‐percentile increase of intracranial carotid artery calcification: −0.01 [95% confidence interval (CI): −0.03, −0.00]). Cognition declined faster across all cognitive tests, specifically for the intracranial carotid artery (p ≤ 0.001).DISCUSSIONArterial calcification is associated with accelerated cognitive decline across domains, especially for arteries closer to the brain. This effect may be attributable to hemodynamic changes preceding neurovascular damage.Highlights
Arterial calcification is related to accelerated cognitive decline.
Arterial calcification closer to the brain exerts the most influence on cognitive decline.
Vascular damage influences cognitive decline across various domains.
Configuration‐Mediated Efficient Non‐Radiative Transition for R848‐Assisted Photothermal Immunotherapy to Inhibit Tumor Growth and Metastasis by An In Situ Tumor Vaccine Strategy
Authors: Dai Jianan , Fang Laiping , Wang Xuan , Hua Jie , Tu Yike , Li Shufang , He Kuo , Hang Lifeng , Xu Yuan , Fang Jin , Wang Lina , Wang Jin , Ma Ping'an , Jiang Guihua 
Published: December 2024

AbstractCancer metastasis remains a critical factor contributing to the current limitations in cancer treatment. Photothermal immunotherapy has emerged as a safe and potent therapeutic approach, demonstrating the capability to suppress tumor growth and metastasis. While researchers have extensively investigated various structural modifications to enhance photothermal conversion performance, the influence of molecular configuration has received comparatively limited attention. In this study, we synthesized two isomers, CZTBT and LVTBT, which possessed distinct configurations. LVTBT, characterized by a flatter molecular configuration, exhibited an extended absorption wavelength and a higher molar extinction coefficient. Its excited state facilitated stronger rotation for non‐radiative transitions, leading to a high photothermal conversion efficiency (PCE) of 36.3 %. When combined with R848, LVTBT@R848 nanoparticles (NPs)‐mediated photothermal immunotherapy functioned as an in situ tumor vaccine, promoting the maturation of dendritic cells (DCs), T cell infiltration, and the differentiation of natural killer (NK) cells and memory T cells, thereby activating the strong immune response. Consequently, it significantly inhibited the growth of both primary and distant tumors, while also limiting lung metastasis. In summary, this study proposed a configuration‐mediated non‐radiative transition strategy for efficient photothermal immunotherapy, advancing the frontiers of organic photothermal agents (OPTAs) design and synthesis.
Mutual Dependence between Membrane Phase Separation and Bacterial Division Protein Dynamics in Synthetic Cell Models
Authors: Kanwa Nishu , Kohyama Shunshi , Fröhlich Leonard , Desai Amogh , Schwille Petra 
Published: December 2024

AbstractCell membranes in bacteria are laterally polarized to produce specific environments for membrane proteins, e.g., proteins involved in cell division which accumulate at mid‐cell or the cell poles. An interesting result of such membrane‐lipid interplay is the reorganization of lipid domains together with membrane‐bound proteins at the onset of cell division, suggesting functional significance of membrane compartments in the cell cycle. Here, by adopting the key bacterial division proteins MinC, MinD, MinE, FtsA and FtsZ as an archetypal spatial patterning system, we present a simple vesicle‐based in vitro model to explore the mutual dependence of protein pattern formation and membrane heterogeneity. Like many other peripheral membrane proteins, Min proteins exhibit preferential binding and macro‐scale pattern formation at Ld domains, which leads to altered oscillation mode selection in phase‐separated membrane compartments (GUVs). Moreover, incorporating bacterial division proteins within phase‐separated GUVs leads to blebbing‐like membrane deformations followed by the reorganization of Lo domains aligning at the neck region of the bleb, which agrees well with the domain rearrangement in bacterial membranes immediately preceding the radial constriction process. Overall, the presented in vitro model system showcases a basic framework to better comprehend the cellular division mechanism in consideration of complex cellular lipid environments.
Cu–Zn Catalysts Derived From ZIF‐8 Applied in Ethynylation of Formaldehyde for 1,4‐Butynediol Synthesis: The Positive Effect of Carbon Layers
Authors: Yang Guihua , Chen Jiali , Yang Linxue , Wang Rui 
Published: December 2024

ABSTRACTCu‐based catalysts applied in ethynylation reaction of formaldehyde for 1,4‐butynediol synthesis has been widely concerned. The activity and stability of Cu‐based catalyst is still a challenging task in this field. Here, Cu–Zn catalysts derived from ZIF‐8 are prepared by a coprecipitation method and applied in ethynylation reaction of formaldehyde. All catalysts were characterized through thermogravimetric, x‐ray diffraction, N2 physical adsorption–desorption, transmission electron microscopy, H2‐temperture‐programmed reduction, x‐ray photoelectron spectroscopy, and Raman and Fourier transform infrared analysis. The effect of calcination temperature of ZIF‐8 on the catalyst structures and ethynylation performances are all investigated. The results show that CuO5h‐ZnO400 catalyst has the best catalytic activity, with a formaldehyde conversion of 98% and 1,4‐butynediol selectivity of 100%. It is mainly due to the presence of highly dispersed and small particle CuO. Moreover, CuO3h‐ZnO400 catalyst prepared by optimized conditions can further improve the stability in ethynylation reaction due to more carbon species on the surface of ZnO. The more carbon contents in Cu–Zn catalyst contribute to the ethynylation activity and stability due to the interaction between Cu and C species favoring Cu2C2 formed. In addition, the ethynylation reaction mechanism catalyzed by Cu–Zn catalyst is illustrated carefully. The Cu–Zn catalysts derived from ZIF‐8 can provide some ideas for the application in ethynylation reaction of formaldehyde for 1,4‐butynediol synthesis.
Preparation of Durable Polytetrafluoroethylene Superhydrophobic Surfaces by Hot Embossing Technique
Authors: Zhou Shuangquan , Wang Xiaojie , Fu Kai , Wu Daming , Gao Xiaochun , Gao Xiaolong 
Published: December 2024

ABSTRACTIn this paper, the surface microstructure of screen meshes with different mesh sizes is processed onto the polytetrafluoroethylene (PTFE) surface using a micro‐nano thermal imprinting technique. Simultaneously, silica microspheres are coated on the screen mesh. After hot embossing, these microspheres adhere to the PTFE surface, enhancing its superhydrophobicity and addressing issues such as shallow microstructure, poor abrasion resistance, and reduced hydrophobicity over prolonged use. Through several comparative experiments, the optimal processing conditions, including temperature, pressure, heating time, mesh aperture, and material thickness, were determined by measuring the contact angle and observing the microstructure. The experimental results indicate that the maximum hydrophobic angle of the PTFE surface is 154°, and the addition of silica microspheres significantly improves the superhydrophobicity and weatherability of the PTFE.
Nimotuzumab and irinotecan synergistically induce ROS‐mediated apoptosis by endoplasmic reticulum stress and mitochondrial‐mediated pathway in cervical cancer
Authors: Teng Fei , Zhao Lujun 
Published: December 2024

AbstractIrinotecan (CPT‐11), a chemotherapeutic agent used to treat several types of cancer, induces cytotoxic effects on healthy cells. The epidermal growth factor receptor (EGFR) plays a crucial role in various forms of cancer. Nimotuzumab (NmAb), a monoclonal antibody that targets the EGFR, is utilized in some countries to treat malignancies that have an overexpression of EGFR. Yet, there is a lack of literature on the potential anticancer properties of the CPT‐11 and NmAb combination on in vitro human cervical cancer cells. This study investigates the apoptosis mode of the CPT‐11 and NmAb combination on cervical HeLa cancer cells. The Annexin V/propidium iodide staining examination demonstrated that the combination of CPT‐11 and NmAb resulted in a decrease in the number of viable cells and more potent induction of cell apoptosis than the effects of CPT‐11 or NmAb alone in HeLa cells. Furthermore, the combined treatment resulted in elevated levels of reactive oxygen species (ROS) and Ca2+ compared to the treatment with CPT‐11 or NmAb alone. Cells that were pretreated with N‐acetyl‐l‐cysteine, a substance that scavenges ROS, and then treated with CPT‐11, NmAb, or a combination of CPT‐11 and NmAb exhibited higher numbers of viable cells compared to those treated with CPT‐11 or NmAb alone. The combination of CPT‐11 and NmAb resulted in significantly higher caspase‐3, ‐8, and ‐9 activity levels than CPT‐11 or NmAb alone, as measured by flow cytometer assay. The combination of CPT‐11 and NmAb in HeLa cells resulted in elevated endoplasmic reticulum stress‐, mitochondria‐, and caspase‐mediated proteins compared to treatment with CPT‐11 or NmAb alone. According to these observations, NmAb enhances the effectiveness of CPT‐11 in fighting cancer by stimulating cell death in the HeLa cells. Therefore, NmAb has the potential to improve the efficacy of CPT‐11 as a future cervical cancer treatment in humans.
Autophagy and Akt‐Stimulated Cellular Proliferation Synergistically Improve Antibody Production in CHO Cells
Authors: Mao Leran , Sonbati Sarah Michelle , Schneider James W. , Robinson Anne S. 
Published: December 2024

ABSTRACTOver the past decade, engineered producer cell lines have led 10‐fold increases in antibody yield, based on an improved understanding of the cellular machinery influencing cell health and protein production. With prospects for further production improvements, increased antibody production would enable a significant cost reduction for life‐saving therapies. In this study, we strategized methods to increase cell viability and the resulting cell culture duration to improve production lifetimes. By overexpressing the cell surface adenosine A2A receptor (A2AR), the Akt pathway was activated, resulting in improved cellular proliferation. Alternatively, by inducing autophagy through temperature downshift, we were able to significantly enhance cellular‐specific productivity, with up to a three‐fold increase in total antibody production as well as three‐fold higher cell‐specific productivity. Interestingly, the expression levels of the autophagy pathway protein Beclin‐1 appeared to correlate best with the total antibody production, of autophagy‐related proteins examined. Thus, during cell clonal development Beclin‐1 levels may serve as a marker to screen for conditions that optimize antibody titer.
Drivers and Challenges in Achieving Corporate Carbon Neutrality—Qualitative Investigation of Carbon Capture, Utilization, and Storage Technologies
Authors: Bhatia Meena , Gugnani Ritika , Yaqub Muhammad Zafar , Agarwal Vaishali 
Published: December 2024

ABSTRACTThe importance of attaining carbon neutrality (CN) has progressively increased in addressing climate change, and consequently, carbon capture, utilization, and storage (CCUS) has emerged as a promising tool in this pursuit. Nevertheless, a dearth of scholarly discourse exists about the factors that drive the implementation of CCUS in the context of corporate CN and the challenges that inhibit the adoption of CCUS for CN. This study aims to bridge this knowledge gap by analyzing open‐ended essays to gain cardinal insights into the experiences and perspectives of companies successfully implementing CCUS for CN. The collected (qualitative) data underwent rigorous analysis to synthesize three distinct dimensions encompassing various aspects of CCUS to achieve CN. The study identifies multiple CCUS components employed to manage carbon emissions effectively. It underscores the five drivers accelerating the adoption of CCUS and classifies them as intrinsic or extrinsic drivers. Various challenges impeding the successful adoption of CCUS have been pinpointed as emanating from financial, human resource, operational, technological, and strategic challenges; technology development and infrastructural challenges, environmental impacts; and social and regulatory challenges. Our research renders valuable theoretical and practical insights based on the prevalent discourse regarding the implementation of CCUS in the field of CN with a comprehensive and inclusive approach.
“The Truth Is, We Must Miss Some”: A Qualitative Study of the Patient Eligibility Screening Process, and Automation Perspectives, for Cancer Clinical Trials
Authors: La Rosa A. , Vaterkowski M. , Cuggia M. , Campillo‐Gimenez B. , Tournigand C. , Baujat B. , Daniel C. , Kempf E. , Lamé G. 
Published: December 2024

ABSTRACTBackgroundRecruitment of cancer patients into clinical trials (CTs) is a challenge. We aimed to explore how patient eligibility assessment is conducted in practice, what factors support or hinder this process, and to assess the potential usefulness of Clinical Trial Recruitment Support Systems (CTRSS) for patient‐to‐trial matching.MethodsWe conducted semi‐structured interviews in France with healthcare professionals involved in cancer CTs and experts on trial recruitment. We focused on the stages in‐between trial feasibility, and patient information and consent. Interviews were recorded, and the transcripts were analyzed thematically. We used the Systems Engineering Initiative for Patient Safety (SEIPS) 2.0 framework to organize our results.ResultsWe interviewed 25 participants. We identified common steps for cancer patient eligibility assessment: prescreening under medical supervision, followed by the validation of patient‐trial matching based on manual chart review. This process built on rich interactions between clinicians, other professionals (clinical research assistants, data scientists, medical coding experts), and patients. Technological factors, mainly related to data infrastructure (both for patient data and trial data), and organizational factors (research culture, incentives, formal and informal research networks) mediated the performance of the recruitment process. Participants had mixed feelings towards CTRSSs; they welcomed automated pre‐screening but insisted on manual verification. Given the necessary collaborative nature of multisite trials, coordinated efforts to support a common data infrastructure could be helpful.ConclusionsMaterial, organizational, and human factors affect cancer patient eligibility assessment for CTs. Patient‐to‐trial matching tools bear potential, but good understanding of the ecosystem, including stakeholders' motivations, is a prerequisite.
Novel Meta‐Diamide Derivatives Bearing Triazole: Design, Synthesis, and Bioactivity
Authors: Yan Chengyi , Liu Jiyong , Xiang Juncheng , Zhang Lei , Wu Daoxin , Liu Minhua 
Published: December 2024

ABSTRACTIn searching for novel insecticide lead, 20 new meta‐diamide compounds containing triazole were designed and synthesized regarding cyproflanilide as lead compound. All the compounds were characterized by 1H NMR, 13C NMR, and High‐resolution mass spectra (HR MS). In preliminary bioassay, we found that one of the compounds: N‐(cyclopropylmethyl)‐N‐(5‐((2,6‐dibromo‐4‐(1,2,2,2‐tetrafluoro‐1‐(trifluoromethyl)ethyl)phenyl)carbamoyl)‐2‐(1H‐1,2,4‐triazol‐1‐yl)phenyl)‐6‐(trifluoromethyl) nicotinamide (16a) had high activity against the target organism Plutella xylostella at 1 mg/L and against the target organism Mythimna separata at 2 mg/L. It is obviously superior to the known compounds Ia and Ib, and it is equivalent to cyproflanilide, and the LC50 values of the compound against P. xylostella and M. separata were 0.90 mg/L and 0.64 mg/L, respectively. It is providing important clues for subsequent structure–activity relationship (SAR) studies. From this, we explored the SAR of these compounds. Through comprehensive analysis of their structural characteristics and insecticidal activity data, we found that different halogen substituents on benzene ring and different aromatic substituents on amino group in the compound had significant effects on insecticidal activity. This has certain guiding significance for further structural optimization and activity enhancement.
The Effects of a Happiness Strengths Intervention on Career Decision‐Making Self‐Efficacy among Career Starters
Authors: Atanasova Nadica , van Woerkom Marianne , Spanouli Andromachi , Botke Jolanda , van Roekel Eeske 
Published: December 2024

AbstractCareer decision‐making self‐efficacy (CDMSE) is an important resource that can reduce the uncertainty that career starters face when transitioning from education to work. However, scant knowledge is available on how CDMSE can be enhanced through character strengths interventions. In this project, an online strengths intervention was developed to help career starters increase their CDMSE by identifying, exploring, appreciating, and using their happiness strengths (curiosity, gratitude, hope, love, and zest). A field experiment was conducted with 55 career starters in the Netherlands who were randomly assigned to an online three‐week intervention (N = 23) or a wait‐list control group (N = 32). Using a pretest‐posttest design, it was found that the intervention positively influenced participants’ CDMSE, particularly their self‐appraisal and problem‐solving levels. Therefore, activating happiness strengths in an online strengths intervention might be used as a brief and effective tool to improve CDMSE (self‐appraisal and problem‐solving) among career starters.
Task‐Specific Design of a Porous Aromatic Framework as an Ultrastable Platform for Enantioselective Organocatalysis
Authors: Liu Kun , Chen Peng , Chen Fan , Sun Fuxing , Lv Pei , Shi Jianghuan , Jiang Yi‐Jun 
Published: December 2024

AbstractA hydroxyl‐tagged porous aromatic framework PAF‐NBU2‐OH was task‐specifically designed and successfully synthesized targeted toward immobilizing chiral catalysts. Using proline‐type compound as model chiral organocatalyst, PAF‐NBU2‐OH was used as a platform to covalently link proline‐type group. The obtained PAF‐immobilized organocatalyst PAF‐NBU2‐OPro featured high chemical stability in different solvents even under very harsh conditions. PAF‐NBU2‐OPro showed excellent catalytic activity, diastereoselectivity and enantioselectivity with complete and easy recyclability when catalyzing the aldol reaction between p‐nitrobenzaldehyde and cyclohexanone, which could undergo at least 30 cycles without any activity, diastereoselectivity or enantioselectivity loss for catalyzing the current reaction.
Improved Piezocatalytic Performance of Cs/KNN Decorated with CuS Nanoparticles for Dye Degradation
Authors: Fan YuLong , Chen YingJie , Zhang Ruihang , Ren JianMei , Wang Guan 
Published: December 2024

AbstractNiobate materials have garnered significant interest in recent years due to their potential applications across various fields. As a special property, piezoelectric catalysts based on niobate remain underexplored, primarily due to their chemical inertness and low piezoelectric activity. In this study, a novel 30 %CuS/0.01 %Cs/KNN composite has been successfully synthesized using a facile hydrothermal method by depositing CuS nanoparticles on the external surface of 0.01 %Cs/KNN cube. By controlling the loading amount of CuS, the catalytic degradation activity of title composite for methyl green MG reached the highest point of 99.5 % under low‐power ultrasound (50 W), which is much higher than that of 0.01 %Cs/KNN (7.5 times) and CuS (5.5 times). In addition, piezoresponse force microscopy (PFM) and electrochemical tests demonstrated that the composites have good piezocatalytic properties. This work provides a meaningful strategy for promoting piezoelectric catalytic degradation using material compounding.