bluuebunny/crossref_metadata_2025_split huggingface dataset:
Preparing plants for improved cold tolerance by priming
Authors: Baier Margarete , Bittner Andras , Prescher Andreas , van Buer Jörn 
Published: July 2018

AbstractCold is a major stressor, which limits plant growth and development in many parts of the world, especially in the temperate climate zones. A large number of experimental studies has demonstrated that not only acclimation and entrainment but also the experience of single short stress events of various abiotic or biotic kinds (priming stress) can improve the tolerance of plants to chilling temperatures. This process, called priming, depends on a stress “memory”. It does not change cold sensitivity per se but beneficially modifies the response to cold and can last for days, months, or even longer. Elicitor factors and antagonists accumulate due to increased biosynthesis or decreased degradation either during or after the priming stimulus. Comparison of priming studies investigating improved tolerance to chilling temperatures highlighted key regulatory functions of ROS/RNS and antioxidant enzymes, plant hormones, especially jasmonates, salicylates, and abscisic acid, and signalling metabolites, such as β‐ and γ‐aminobutyric acid (BABA and GABA) and melatonin. We conclude that these elicitors and antagonists modify local and systemic cold tolerance by integration into cold‐induced signalling cascades.
Management of afebrile neonates with pustules and vesicles in a pediatric emergency department
Authors: Manice Christina S. , Planet Paul J. , Chase Herbert S. , Lauren Christine T. 
Published: July 2018

AbstractObjectivesTo assess the management and outcomes of vesicles and pustules in afebrile neonates presenting to the pediatric emergency department.MethodsUsing International Classification of Diseases, Ninth Revision, codes, we identified patients 0‐60 days old presenting to our pediatric emergency department from 2008 to 2015 with a possible diagnosis of pustules or vesicles. We then used natural language processing followed by manual chart review to identify afebrile neonates with pustules or vesicles. We collected clinical data from the electronic medical record. We also assessed current practice patterns for neonatal pustules or vesicles using a survey administered to attending physicians.ResultsOf the 971 possible cases identified using International Classification of Diseases, Ninth Revision, codes for fluid‐filled lesions, only 64 patients had vesicles (n = 9) and pustules (n = 55). One‐third (22/64) of afebrile neonates with pustules and vesicles were admitted to the hospital and received empiric parenteral therapy. Admission, parenteral antibiotics, and antiviral therapy were more common in neonates presenting with vesicles than in those with pustules alone. Apart from 2 presumed blood culture contaminants, there were no positive blood or cerebrospinal fluid cultures. Two patients had positive urine cultures. Institutional survey data showed practice patterns consistent with these retrospective results.ConclusionAlthough one‐third of neonates with pustules and vesicles were admitted to the hospital and received parenteral therapy, there were no cerebrospinal fluid or blood infections or any confirmed evidence of herpes simplex virus disease. These findings suggest that afebrile, well‐appearing neonates presenting with pustules alone may not need a full serious bacterial infection examination. Larger studies are needed to confirm these findings and assess outcomes, especially in afebrile neonates with vesicles.
Germline BRCA1‐associated protein 1 mutation presenting as BAP1 inactivated melanocytic nevi in a child of a father with fatal paraganglioma
Authors: Watchorn Richard E. , Calonje Eduardo , Taibjee Saleem M. 
Published: July 2018

AbstractBRCA1‐associated protein 1(BAP1) inactivated melanocytic nevi are pink to tan and dome‐shaped in clinical appearance, resembling dermal nevi, but with distinct histologic features of two melanocytic subpopulations: larger atypical melanocytes and nests of smaller, blander nevoid melanocytes. Pedigrees with BAP1 mutations are at greater risk of various malignancies. We report the case of a 16‐year‐old boy with multiple benign‐appearing nevi, all demonstrating loss of BAP1 on immunohistochemistry. History revealed that his father had died of paraganglioma, which is also associated with BAP1 mutations.
Inflammatory linear verrucous epidermal nevus associated with erosive monoarthritis
Authors: Bertolin‐Colilla Marta , Alcalá Rebeca , Deza Gustavo , Polino Luciano , Gallardo Fernando , Pujol Ramon M. 
Published: July 2018

AbstractInflammatory linear verrucous epidermal nevus and linear psoriasis are different entities with clinical and histopathologic similarities. Isolated reports of inflammatory linear verrucous epidermal nevus with concomitant psoriasis or a positive family history of psoriasis have been described, and the possibility that inflammatory linear verrucous epidermal nevus may be a mosaic form of cutaneous psoriasis has been postulated. We report a 17‐year‐old boy with a congenital, linear, erythematous, keratotic plaque on the dorsum of the fifth finger of the left hand with ipsilateral nail dystrophy. Histopathologic examination showed epidermal hyperplasia with alternating orthokeratosis and parakeratosis. During follow‐up, he developed erosive monoarthritis of the distal interphalangeal joint. This case seems to confirm the association between inflammatory linear verrucous epidermal nevus and arthritis and supports a possible relationship between inflammatory linear verrucous epidermal nevus and psoriasis.
Extracellular vesicles isolated from Toxoplasma gondii induce host immune response
Authors: Silva Valeria Oliveira , Maia Marta Marques , Torrecilhas Ana Claudia , Taniwaki Noemi Nosomi , Namiyama Gislene Mitsue , Oliveira Katia Cristina , Ribeiro Kleber Silva , Toledo Maytê dos Santos , Xander Patricia , Pereira‐Chioccola Vera Lucia 
Published: July 2018

SummaryThis study established a protocol to purify Toxoplasma gondii tachyzoite microvesicles and exosomes, called as extracellular vesicles (EVs). In addition, the investigations were conducted to determine the kinetic of EV release by tachyzoites and whether EV proteins are able to modulate the host immune response. The particle size and concentration released by tachyzoites in culture medium at different incubation‐period were characterized by nanoparticle tracking analysis. Tachyzoites (1 × 106) released around 4.37 ± 0.81 × 108 EVs/mL/h, with size varying between 138.2 and 171.9 nm. EVs released into the medium were purified by gel‐exclusion chromatography and screened by ELISA, using a pool of human positive sera for toxoplasmosis. EV‐fractions contained high concentration of proteins, and EVs were analyzed by scanning and transmission electron microscopies. Tachyzoites released EVs into the culture medium throughout all membrane surface, and these vesicles contain small RNAs/miRNA. Pooled sera from chronically infected human or mice (infected with 2 different T. gondii strains) recognized distinct EV electrophoretic patterns in immunoblotting. T. gondii EVs significantly induced IL‐10, TNF‐α and iNOS in murine macrophages. In conclusion, this study shows that T. gondii secrete/excrete EVs (microvesicles and exosomes) contain miRNA and they were immunologically recognized by host immune response.
The influence of spaying and its timing relative to the onset of puberty on urinary and general behaviour in Labrador Retrievers
Authors: Balogh Orsolya , Borruat Natalie , Andrea Meier Angela , Hartnack Sonja , Reichler Iris M. 
Published: July 2018

AbstractBenefits and risks of spaying on the behaviour of female dogs are controversially discussed. Increased aggressiveness and male urinary behaviour were postulated to be the consequence of masculinization after spaying in some female dogs. To investigate if spaying or its timing relative to the onset of puberty may have a masculinization effect, urinary behaviour, that is, frequency of urination, urinary posture and ground scratching after urination were recorded in 58 female Labrador Retrievers during 15 min of a daily walk with their owners. General behaviour of the dogs during the walks was assessed using an owner questionnaire. Data were analysed for age, reproductive status, lifetime of ovary exposure and/or time interval since spaying. Urinary behaviour of intact females (n = 12) and dogs spayed before (n = 17) or after (n = 29) puberty was similar and not influenced by age, lifetime of ovary exposure and/or time interval since spaying. Owners of spayed dogs described more frequent or more intense fear reaction in their animals in response to loud noises, unfamiliar objects approaching on or near the sidewalk, or if they were approached by unknown dogs barking, growling or jumping. In conclusion, we found no evidence of a masculinization effect after spaying on urinary behaviour in female Labrador Retrievers. In contrast to popular belief, gonadectomy did not inevitably result in a behaviourally more stable dog. Extrapolation of our findings from female Labrador Retrievers to other breeds should be performed with caution, as the effect of spaying on behaviour may differ among dog breeds.
Why does a woman need to become a man in order to become a Buddha?: Past investigations, new leads
Authors: Balkwill Stephanie 
Published: July 2018

AbstractThis paper puts forth a new interpretation of the problem of the ineligibility of the female body for Buddhahood as it is seen in the texts of the Mahāyāna tradition of Buddhism. Recognizing that advanced female practitioners in Mahāyāna sūtras both lecture their detractors on the emptiness of physical forms while simultaneously changing their female forms to male ones, this paper provides an interpretation of the available textual material that reads the actions of such female practitioners as consonant and not contradictory. In so doing, this paper offers an innovative reading of the famous story of the Dragon King's Daughter from the Lotus Sūtra, one that sees it in conversation with a lesser known text specifically on the topic of female‐to‐male sex change on the path to Buddhahood, the Sūtra on Transforming the Female Form.Video Abstract available at https://youtu.be/iK8sJNq4y5s
Is the consumption of fast foods associated with asthma or other allergic diseases?
Authors: Wang Cheng S. , Wang Ji , Zhang Xin , Zhang Li , Zhang Hong P. , Wang Lei , Wood Lisa G. , Wang Gang 
Published: July 2018

ABSTRACTThe associations between the consumption of fast foods and asthma or allergic diseases have not been clarified. The aim of this study was to determine whether fast foods consumption is associated with asthma or allergic diseases. Databases were searched up to February 2018. Studies investigating the associations between fast foods consumption and asthma or allergic diseases were considered eligible. Included studies were assessed for quality using standardized critical appraisal checklists. The quality scores were 5.33 ± 1.16 in case–control studies and 5.69 ± 1.55 in cross‐sectional studies. Adjusted odds ratios (aOR) with 95% confidence interval (CI) were pooled. Sixteen studies (13 cross‐sectional and 3 case–control studies) were included. The consumption of fast foods was significantly related to current asthma (aOR: 1.58; 95% CI: 1.17–2.13 for case–control study and aOR: 1.58; 95% CI: 1.10–2.26 for cross‐sectional studies), severe asthma (aOR: 1.34; 95% CI: 1.23–1.46), asthma ever (aOR: 1.36; 95% CI: 1.06–1.75), current wheeze (aOR: 1.21; 95% CI: 1.16–1.27), wheeze ever (aOR: 1.65; 95% CI: 1.07–2.52), physician‐diagnosed allergic rhinitis (odds ratio: 1.43; 95% CI: 1.05–1.95), severe eczema (aOR: 1.51; 95% CI: 1.16–1.96) and severe rhino‐conjunctivitis (aOR: 1.54; 95% CI: 1.18–2.00). The consumption of hamburgers was associated with current asthma (aOR: 1.59; 95% CI: 1.13–2.25), severe asthma (aOR: 1.34; 95% CI: 1.23–1.46), asthma ever (aOR: 1.47; 95% CI: 1.13–1.92), severe eczema (aOR: 1.51; 95% CI: 1.16–1.96), severe rhino‐conjunctivitis (aOR: 1.54; 95% CI: 1.18–2.00) and rhino‐conjunctivitis (aOR: 1.21; 95% CI: 1.15–1.27). The consumption of fast foods, especially hamburgers, ≥3 times/week, was more likely to be associated with severe asthma and current wheeze compared with the consumption of 1–2 times/week (both P < 0.001). In conclusion, the consumption of fast foods, particularly hamburgers, correlates to asthma in a dose–response pattern, which needs to be further validated in longitudinal and interventional studies.
A Risk Assessment Approach for Evaluating the Impact of Toxic Contaminants Released Indoors by Considering Various Emergency Ventilation and Evacuation Strategies
Authors: Zeng Lingjie , Gao Jun , Wang Qiong , Chang Le 
Published: July 2018

AbstractThe release of toxic airborne contaminants resulting from terrorist attacks on buildings can lead to disastrous consequences. To evaluate and reduce the effects of these emergencies, various methods and models have been developed in the past few years. Such work has provided effective tools for the building management system to do risk assessment of the contaminated areas. Although risk analysis methods to describe the contaminant dispersion scenarios made significant progress, these approaches did not generally consider the releasing scenario occurring in the ventilation system and the effect of human behavior during the developing process of an emergency event. Emergency strategies chosen by the decisionmaker are not always associated with the early‐warning system, such as the sensor monitoring network and the source identification system inside the building. This study aims to provide a risk assessment model considering both the variation of contaminant concentration and occupant distribution after the release of toxic agents to obtain the exposure risk for people indoors. The contaminant dispersion is simulated using computational fluid dynamics. The evacuation process for people is modeled using Pathfinder, and the exposure risk for occupants under various emergency strategies is calculated using the efficiency factor of the contaminant source. The results of the exposure risk for 40 basic cases are discussed, and the optimal ventilation mode for these specific cases is recommended. Next, the impact of the variation of human behavior, contaminant detection time needed by sensors, and source identification time needed by inverse modeling on the exposure risk for people indoor is studied. The uncertainty and reproducibility of the numerical simulations are emphatically discussed in the Supporting Information.
Hamiltonian Structure and a Variational Principle for Grounded Abyssal Flow on a Sloping Bottom in a Mid‐Latitude β‐Plane
Authors: Swaters Gordon E. 
Published: July 2018

AbstractObservations, numerical simulations, and theoretical scaling arguments suggest that in mid‐latitudes, away from the high‐latitude source regions and the equator, the meridional transport of abyssal water masses along a continental slope correspond to geostrophic flows that are gravity or density driven and topographically steered. These dynamics are examined using a nonlinear reduced‐gravity geostrophic model that describes grounded abyssal meridional flow over sloping topography that crosses the planetary vorticity gradient. It is shown that this model possesses a noncanonical Hamiltonian formulation. General nonlinear steady solutions to the model can be obtained for arbitrary bottom topography. These solutions correspond to nonparallel shear flows that flow across the planetary vorticity gradient. If the in‐flow current along the poleward boundary is strictly equatorward, then no shock can form in the solution in the mid‐latitude domain. It is also shown that the steady solutions satisfy the first‐order necessary conditions for an extremal to a suitably constrained potential energy functional. Sufficient conditions for the definiteness of the second variation of the constrained energy functional are examined. The theory is illustrated with a nonlinear steady solution corresponding to an abyssal flow with upslope and down slope groundings in the height field.
DNA-induced liquid phase condensation of cGAS activates innate immune signaling
Authors: Du Mingjian , Chen Zhijian J. 
Published: July 2018

Liquid droplets step on the cGAS
Spontaneous partitioning of a homogeneous solution of molecules, or liquid-phase separation, underlies the formation of cellular bodies from P granules to nucleoli. Essentially, dense-phase liquid droplets act like cellular compartments. Du and Chen show that DNA binding to its cytoplasmic sensor, cyclic GMP–AMP synthase (cGAS), results in liquid droplets containing activated cGAS (see the Perspective by Ablasser). This phenomenon occurs through multivalent interactions, augmented by zinc, between DNA binding domains on cGAS and DNA in a length-dependent manner. Binding triggers a switchlike reaction that concentrates the enzyme and reactants to enhance STING-dependent interferon responses.

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Central nervous system modulates the neuromechanical delay in a broad range for the control of muscle force
Authors: Del Vecchio A. , Úbeda A. , Sartori M. , Azorín J. M. , Felici F. , Farina D. 
Published: July 2018

Force is generated by muscle units according to the neural activation sent by motor neurons. The motor unit is therefore the interface between the neural coding of movement and the musculotendinous system. Here we propose a method to accurately measure the latency between an estimate of the neural drive to muscle and force. Furthermore, we systematically investigate this latency, which we refer to as the neuromechanical delay (NMD), as a function of the rate of force generation. In two experimental sessions, eight men performed isometric finger abduction and ankle dorsiflexion sinusoidal contractions at three frequencies and peak-to-peak amplitudes {0.5, 1, and 1.5 Hz; 1, 5, and 10 of maximal force [%maximal voluntary contraction (MVC)]}, with a mean force of 10% MVC. The discharge timings of motor units of the first dorsal interosseous (FDI) and tibialis anterior (TA) muscle were identified by high-density surface EMG decomposition. The neural drive was estimated as the cumulative discharge timings of the identified motor units. The neural drive predicted 80 ± 0.4% of the force fluctuations and consistently anticipated force by 194.6 ± 55 ms (average across conditions and muscles). The NMD decreased nonlinearly with the rate of force generation ( R2 = 0.82 ± 0.07; exponential fitting) with a broad range of values (from 70 to 385 ms) and was 66 ± 0.01 ms shorter for the FDI than TA ( P < 0.001). In conclusion, we provided a method to estimate the delay between the neural control and force generation, and we showed that this delay is muscle-dependent and is modulated within a wide range by the central nervous system. NEW & NOTEWORTHY The motor unit is a neuromechanical interface that converts neural signals into mechanical force with a delay determined by neural and peripheral properties. Classically, this delay has been assessed from the muscle resting level or during electrically elicited contractions. In the present study, we introduce the neuromechanical delay as the latency between the neural drive to muscle and force during variable-force contractions, and we show that it is broadly modulated by the central nervous system.
Exercise preconditioning diminishes skeletal muscle atrophy after hindlimb suspension in mice
Authors: Theilen Nicholas T. , Jeremic Nevena , Weber Gregory J. , Tyagi Suresh C. 
Published: July 2018

The aim of the present study was to investigate whether short-term, concurrent exercise training before hindlimb suspension (HLS) prevents or diminishes both soleus and gastrocnemius atrophy and to analyze whether changes in mitochondrial molecular markers were associated. Male C57BL/6 mice were assigned to control at 13 ± 1 wk of age, 7-day HLS at 12 ± 1 wk of age (HLS), 2 wk of exercise training before 7-day HLS at 10 ± 1 wk of age (Ex+HLS), and 2 wk of exercise training at 11 ± 1 wk of age (Ex) groups. HLS resulted in a 27.1% and 21.5% decrease in soleus and gastrocnemius muscle weight-to-body weight ratio, respectively. Exercise training before HLS resulted in a 5.6% and 8.1% decrease in soleus and gastrocnemius weight-to-body weight ratio, respectively. Exercise increased mitochondrial biogenesis- and function-associated markers and slow myosin heavy chain (SMHC) expression, and reduced fiber-type transitioning marker myosin heavy chain 4 (Myh4). Ex+HLS revealed decreased reactive oxygen species (ROS) and oxidative stress compared with HLS. Our data indicated the time before an atrophic setting, particularly caused by muscle unloading, may be a useful period to intervene short-term, progressive exercise training to prevent skeletal muscle atrophy and is associated with mitochondrial biogenesis, function, and redox balance. NEW & NOTEWORTHY Mitochondrial dysfunction is associated with disuse-induced skeletal muscle atrophy, whereas exercise is known to increase mitochondrial biogenesis and function. Here we provide evidence of short-term concurrent exercise training before an atrophic event protecting skeletal muscle from atrophy in two separate muscles with different, dominant fiber-types, and we reveal an association with the adaptive changes of mitochondrial molecular markers to exercise.
Replication Stress Drives Constitutive Activation of the DNA Damage Response and Radioresistance in Glioblastoma Stem-like Cells
Authors: Carruthers Ross D. , Ahmed Shafiq U. , Ramachandran Shaliny , Strathdee Karen , Kurian Kathreena M. , Hedley Ann , Gomez-Roman Natividad , Kalna Gabriela , Neilson Mathew , Gilmour Lesley , Stevenson Katrina H. , Hammond Ester M. , Chalmers Anthony J. 
Published: July 2018

Abstract
Glioblastoma (GBM) is a lethal primary brain tumor characterized by treatment resistance and inevitable tumor recurrence, both of which are driven by a subpopulation of GBM cancer stem–like cells (GSC) with tumorigenic and self-renewal properties. Despite having broad implications for understanding GSC phenotype, the determinants of upregulated DNA-damage response (DDR) and subsequent radiation resistance in GSC are unknown and represent a significant barrier to developing effective GBM treatments. In this study, we show that constitutive DDR activation and radiation resistance are driven by high levels of DNA replication stress (RS). CD133+ GSC exhibited reduced DNA replication velocity and a higher frequency of stalled replication forks than CD133− non-GSC in vitro; immunofluorescence studies confirmed these observations in a panel of orthotopic xenografts and human GBM specimens. Exposure of non-GSC to low-level exogenous RS generated radiation resistance in vitro, confirming RS as a novel determinant of radiation resistance in tumor cells. GSC exhibited DNA double-strand breaks, which colocalized with “replication factories” and RNA: DNA hybrids. GSC also demonstrated increased expression of long neural genes (&gt;1 Mbp) containing common fragile sites, supporting the hypothesis that replication/transcription collisions are the likely cause of RS in GSC. Targeting RS by combined inhibition of ATR and PARP (CAiPi) provided GSC-specific cytotoxicity and complete abrogation of GSC radiation resistance in vitro. These data identify RS as a cancer stem cell–specific target with significant clinical potential.
Significance: These findings shed new light on cancer stem cell biology and reveal novel therapeutics with the potential to improve clinical outcomes by overcoming inherent radioresistance in GBM. Cancer Res; 78(17); 5060–71. ©2018 AACR.