bluuebunny/crossref_metadata_2025_split huggingface dataset:
‘I want to make myself useful’: the value of nature-based adult day services in urban areas for people with dementia and their family carers
Authors: de Bruin Simone R. , Buist Yvette , Hassink Jan , Vaandrager Lenneke 
Published: October 2019

AbstractNature-based adult day services (ADSs) in urban areas are relatively new services in the Netherlands. Since knowledge about these services is still scarce, this study aimed to elucidate their value for people with dementia and their family carers in terms of health and wellbeing. We interviewed 39 people with dementia attending nature-based ADSs in urban areas and their family carers, and 17 providers of these services. Respondents indicated that nature-based ADSs in urban areas positively affected the health and wellbeing of people with dementia. According to them, these services support contact with nature and animals, activity engagement, physical activity, structure, social interactions, healthy eating, a sense of meaning in life and a focus on normal daily life. Respondents further indicated that these services stimulate respite, reassurance and maintenance of family carers’ own activities and social contacts. We conclude that nature-based ADSs in urban areas have a wide range of benefits that might affect the health and wellbeing of people with dementia and their family carers. Worldwide, demand is growing for innovative practices in dementia care. It is therefore worthwhile monitoring the development of dementia care innovations, such as nature-based ADSs, and for countries to exchange lessons learned from these services.
Topological twists in the Syrian conflict: Re-thinking space through bread
Authors: Martínez José Ciro 
Published: October 2019

AbstractThis article seeks to question the epistemological monopoly of territory and scale in analyses of the Syrian conflict. It does so to both challenge static conceptualisations of space in the study of politics and analyse how seemingly remote actors influence wartime outcomes. Since 2011, NGOs, government bodies, and merchants have worked to connect Damascus to Tehran, Idlib to Istanbul, London to Dara‘a. These connections have proven crucial to the reliable supply of food, funds, and firepower. Yet rather than reveal the importance of foreign patrons or proxies on the ground, such dynamics speak to a world in which relationships matter more than distance, practices more than geopolitical position ora prioriforms of alliance. Drawing on the work of John Allen, I suggest why thinking topologically about these dynamics better equips us to understand the political outcomes they help engender. To demonstrate the promise of this approach, I hone in on the partnerships, intermediaries, and connections that shape performances of political authority in Syria by examining one object crucial to its enactment: bread.
The Infinite limit of random permutations avoiding patterns of length three
Authors: Pinsky Ross G. 
Published: October 2019

AbstractFor $$\tau \in {S_3}$$, let $$\mu _n^\tau $$ denote the uniformly random probability measure on the set of $$\tau $$-avoiding permutations in $${S_n}$$. Let $${\mathbb {N}^*} = {\mathbb {N}} \cup \{ \infty \} $$ with an appropriate metric and denote by $$S({\mathbb{N}},{\mathbb{N}^*})$$ the compact metric space consisting of functions $$\sigma {\rm{ = }}\{ {\sigma _i}\} _{i = 1}^\infty {\rm{ }}$$ from $$\mathbb {N}$$ to $${\mathbb {N}^ * }$$ which are injections when restricted to $${\sigma ^{ - 1}}(\mathbb {N})$$; that is, if $${\sigma _i}{\rm{ = }}{\sigma _j}$$, $$i \ne j$$, then $${\sigma _i} = \infty $$. Extending permutations $$\sigma \in {S_n}$$ by defining $${\sigma _j} = j$$, for $$j \gt n$$, we have $${S_n} \subset S({\mathbb{N}},{{\mathbb{N}}^*})$$. For each $$\tau \in {S_3}$$, we study the limiting behaviour of the measures $$\{ \mu _n^\tau \} _{n = 1}^\infty $$ on $$S({\mathbb{N}},{\mathbb{N}^*})$$. We obtain partial results for the permutation $$\tau = 321$$ and complete results for the other five permutations $$\tau \in {S_3}$$.
Survey of Saturn's Magnetopause and Bow Shock Positions Over the Entire Cassini Mission: Boundary Statistical Properties and Exploration of Associated Upstream Conditions
Authors: Jackman C. M. , Thomsen M. F. , Dougherty M. K. 
Published: October 2019

AbstractThe Cassini spacecraft orbited the planet Saturn from July 2004 to September 2017, and its varied orbital trajectory took it across the magnetopause and bow shock boundaries multiple times, at varying radial distances, local times, latitudes, and phases of the solar cycle. Here we present a comprehensive list of these boundary crossings, derived primarily using data from the Cassini magnetometer instrument, with cross‐validation against the electron spectrometer data where available. There are a multitude of scientific avenues for exploitation of this list. In this work, we examine the variability in boundary location and use the crossing times in concert with models of the bow shock and magnetopause to infer the upstream solar wind dynamic pressure at the times of crossings. This analysis allows us to understand the limitations of the Cassini trajectory for studying boundary physics under a range of solar wind driving conditions. In addition, rapid traversals of the magnetosheath are used to estimate the range of speeds of boundary motion.
Ectomycorrhizal fungal communities in ice-age relict forests of Pinus pumila on nine mountains correspond to summer temperature
Authors: Koizumi Takahiko , Nara Kazuhide 
Published: October 2019

Abstract
Ectomycorrhizal (ECM) fungi are critical symbionts of major forest trees, and their communities are affected by various environmental factors including temperature. However, previous knowledge concerning temperature effects does not exclude the effects of host species and coexisting plants, which usually change with temperature, and should be rigorously tested under the same vegetation type. Herein we examined ECM fungal communities in ice-age relict forests dominated by a single host species (Pinus pumila) distributed on nine mountains across &gt;1000 km in Japan. Direct sequencing of rDNA ITS regions identified 154 ECM fungal species from 4134 ECM root-tip samples. Gradient analyses revealed a large contribution of temperature, especially summer temperature, to ECM fungal communities. Additionally, we explored global sequence records of each fungal species to infer its potential temperature niche, and used it to estimate the temperature of the observed communities. The estimated temperature was significantly correlated with the actual temperature of the research sites, especially in summer seasons, indicating inherent temperature niches of the fungal components could determine their distribution among the sites. These results indicate that temperature is still a significant determinant in structuring ECM fungal communities after excluding the effects of host species and coexisting plants. The results also imply that the rising temperature under global warming may have been affecting soil microbes unnoticeably, while such microbial community change may have been contributing to the resilience of the same vegetation.
SIRPα expression delineates subsets of intratumoral monocyte/macrophages with different functional and prognostic impact in follicular lymphoma
Authors: Chen Ya-Ping , Kim Hyo Jin , Wu Hongyan , Price-Troska Tammy , Villasboas Jose C. , Jalali Shahrzad , Feldman Andrew L. , Novak Anne J. , Yang Zhi-Zhang , Ansell Stephen M. 
Published: October 2019

AbstractSignal regulatory protein-α (SIRPα) is a key member of the “do-not-eat-me” signaling pathway, but its biological role and clinical relevance in B-cell NHL is relatively unknown. Using biopsy specimens from follicular lymphoma (FL), we identified three subsets (CD14+SIRPαhi, CD14−SIRPαlow, and CD14−SIRPαneg) of monocyte/macrophages (Mo/MΦ) based on CD14 and SIRPα expression. CD14+SIRPαhi cells expressed common Mo/MΦ markers; exhibited characteristic differentiation, migration, and phagocytosis; and suppressed T-cell function. CD14−SIRPαlow cells expressed fewer typical Mo/MΦ markers; migrated less and phagocytosed tumor cells less efficiently; and stimulated rather than suppressed T-cell function. Interestingly, the CD14−SIRPαneg subset expressed distinct Mo/MΦ markers compared to the other two subsets; had limited ability to migrate and phagocytose; but stimulated T-cell function. When using SIRPα-Fc to block the interaction between SIRPα and CD47, alone or in combination with rituximab, phagocytosis of tumor cells was differentially increased in the three Mo/MΦ subsets. Clinically, increased numbers of CD14+SIRPαhi cells were associated with an inferior survival in FL. In contrast, increased numbers of the CD14−SIRPαlow subset appeared to correlate with a better survival. Taken together, our results show that SIRPα expression delineates unique subsets of intratumoral Mo/MΦs with differing prognostic importance.
Targeting the COX2/MET/TOPK signaling axis induces apoptosis in gefitinib-resistant NSCLC cells
Authors: Xiao Juanjuan , Wang Fei , Lu Hui , Xu Sanpeng , Zou Ling , Tian Qin , Fu Yang , Lin Xuan , Liu Lin , Yuan Ping , Ni Xiaofang , Ma Tengfei , Zeng Fanfan , Xue Peipei , Xiu Ruijuan , Zhang Jianmin , Ji Xinying , Hu Hongbo , Lu Shangyun , Dai Hongtian , Li Yuan , Chu Qian , Zhao Xia , Duan Qiuhong , Zhu Feng 
Published: October 2019

AbstractMET overactivation is one of the crucial reasons for tyrosine kinase inhibitor (TKI) resistance, but the mechanisms are not wholly clear. Here, COX2, TOPK, and MET expression were examined inEGFR-activating mutated NSCLC by immunohistochemical (IHC) analysis. The relationship between COX2, TOPK, and MET was explored in vitro and ex vivo. In addition, the inhibition of HCC827GR cell growth by combining COX2 inhibitor (celecoxib), TOPK inhibitor (pantoprazole), and gefitinib was verified ex vivo and in vivo. We found that COX2 and TOPK were highly expressed inEGFR-activating mutated NSCLC and the progression-free survival (PFS) of triple-positive (COX2, MET, and TOPK) patients was shorter than that of triple-negative patients. Then, we observed that the COX2-TXA2signaling pathway modulated MET through AP-1, resulting in an inhibition of apoptosis in gefitinib-resistant cells. Moreover, we demonstrated that MET could phosphorylate TOPK at Tyr74 and then prevent apoptosis in gefitinib-resistant cells. In line with these findings, the combination of celecoxib, pantoprazole, and gefitinib could induce apoptosis in gefitinib-resistant cells and inhibit tumor growth ex vivo and in vivo. Our work reveals a novel COX2/MET/TOPK signaling axis that can prevent apoptosis in gefitinib-resistant cells and suggests that a triple combination of FDA-approved drugs would provide a low-cost and practical strategy to overcome gefitinib resistance.
MicroRNA-3163 targets ADAM-17 and enhances the sensitivity of hepatocellular carcinoma cells to molecular targeted agents
Authors: Yang Bin , Wang Chunping , Xie Hui , Wang Yiwu , Huang Jiagan , Rong Yihui , Zhang Huixin , Kong Huifang , Yang Yongping , Lu Yinying 
Published: October 2019

AbstractMolecular targeted agents, such as sorafenib, remain the only choice of an antitumor drug for the treatment of advanced hepatocellular carcinoma (HCC). The Notch signaling pathway plays central roles in regulating the cellular injury/stress response, anti-apoptosis, or epithelial–mesenchymal transition process in HCC cells, and is a promising target for enhancing the sensitivity of HCC cells to antitumor agents. The ADAM metalloprotease domain-17 (ADAM-17) mediates the cleavage and activation of Notch protein. In the present study, microRNA-3163 (miR-3163), which binds to the 3′-untranslated region of ADAM-17, was screened using online methods. miRDB and pre-miR-3163 sequences were prepared into lentivirus particles to infect HCC cells. miR-3163 targeted ADAM-17 and inhibited the activation of the Notch signaling pathway. Infection of HCC cells with miR-3163 enhanced their sensitivity to molecular targeted agents, such as sorafenib. Therefore, miR-3163 may contribute to the development of more effective strategies for the treatment of advanced HCC.
Genetic dissection of complex behaviour traits in German Shepherd dogs
Authors: Friedrich Juliane , Strandberg Erling , Arvelius Per , Sánchez-Molano E. , Pong-Wong Ricardo , Hickey John M. , Haskell Marie J. , Wiener Pamela 
Published: October 2019

AbstractA favourable genetic structure and diversity of behavioural features highlights the potential of dogs for studying the genetic architecture of behaviour traits. However, behaviours are complex traits, which have been shown to be influenced by numerous genetic and non-genetic factors, complicating their analysis. In this study, the genetic contribution to behaviour variation in German Shepherd dogs (GSDs) was analysed using genomic approaches. GSDs were phenotyped for behaviour traits using the established Canine Behavioural Assessment and Research Questionnaire (C-BARQ). Genome-wide association study (GWAS) and regional heritability mapping (RHM) approaches were employed to identify associations between behaviour traits and genetic variants, while accounting for relevant non-genetic factors. By combining these complementary methods we endeavoured to increase the power to detect loci with small effects. Several behavioural traits exhibited moderate heritabilities, with the highest identified for Human-directed playfulness, a trait characterised by positive interactions with humans. We identified several genomic regions associated with one or more of the analysed behaviour traits. Some candidate genes located in these regions were previously linked to behavioural disorders in humans, suggesting a new context for their influence on behaviour characteristics. Overall, the results support dogs as a valuable resource to dissect the genetic architecture of behaviour traits and also highlight the value of focusing on a single breed in order to control for background genetic effects and thus avoid limitations of between-breed analyses.
A high-quality Actinidia chinensis (kiwifruit) genome
Authors: Wu Haolin , Ma Tao , Kang Minghui , Ai Fandi , Zhang Junlin , Dong Guanyong , Liu Jianquan 
Published: October 2019

Abstract
Actinidia chinensis (kiwifruit) is a perennial horticultural crop species of the Actinidiaceae family with high nutritional and economic value. Two versions of the A. chinensis genomes have been previously assembled, based mainly on relatively short reads. Here, we report an improved chromosome-level reference genome of A. chinensis (v3.0), based mainly on PacBio long reads and Hi-C data. The high-quality assembled genome is 653 Mb long, with 0.76% heterozygosity. At least 43% of the genome consists of repetitive sequences, and the most abundant long terminal repeats were further identified and account for 23.38% of our novel genome. It has clear improvements in contiguity, accuracy, and gene annotation over the two previous versions and contains 40,464 annotated protein-coding genes, of which 94.41% are functionally annotated. Moreover, further analyses of genetic collinearity revealed that the kiwifruit genome has undergone two whole-genome duplications: one affecting all Ericales families near the K-T extinction event and a recent genus-specific duplication. The reference genome presented here will be highly useful for further molecular elucidation of diverse traits and for the breeding of this horticultural crop, as well as evolutionary studies with related taxa.
Evaluation of integrin αvβ6 cystine knot PET tracers to detect cancer and idiopathic pulmonary fibrosis
Authors: Kimura Richard H. , Wang Ling , Shen Bin , Huo Li , Tummers Willemieke , Filipp Fabian V. , Guo Haiwei Henry , Haywood Thomas , Abou-Elkacem Lotfi , Baratto Lucia , Habte Frezghi , Devulapally Rammohan , Witney Timothy H. , Cheng Yan , Tikole Suhas , Chakraborti Subhendu , Nix Jay , Bonagura Christopher A. , Hatami Negin , Mooney Joshua J. , Desai Tushar , Turner Scott , Gaster Richard S. , Otte Andrea , Visser Brendan C. , Poultsides George A. , Norton Jeffrey , Park Walter , Stolowitz Mark , Lau Kenneth , Yang Eric , Natarajan Arutselvan , Ilovich Ohad , Srinivas Shyam , Srinivasan Ananth , Paulmurugan Ramasamy , Willmann Juergen , Chin Frederick T. , Cheng Zhen , Iagaru Andrei , Li Fang , Gambhir Sanjiv S. 
Published: October 2019

AbstractAdvances in precision molecular imaging promise to transform our ability to detect, diagnose and treat disease. Here, we describe the engineering and validation of a new cystine knot peptide (knottin) that selectively recognizes human integrin αvβ6 with single-digit nanomolar affinity. We solve its 3D structure by NMR and x-ray crystallography and validate leads with 3 different radiolabels in pre-clinical models of cancer. We evaluate the lead tracer’s safety, biodistribution and pharmacokinetics in healthy human volunteers, and show its ability to detect multiple cancers (pancreatic, cervical and lung) in patients at two study locations. Additionally, we demonstrate that the knottin PET tracers can also detect fibrotic lung disease in idiopathic pulmonary fibrosis patients. Our results indicate that these cystine knot PET tracers may have potential utility in multiple disease states that are associated with upregulation of integrin αvβ6.
Origin of two-band chorus in the radiation belt of Earth
Authors: Li Jinxing , Bortnik Jacob , An Xin , Li Wen , Angelopoulos Vassilis , Thorne Richard M. , Russell Christopher T. , Ni Binbin , Shen Xiaochen , Kurth William S. , Hospodarsky George B. , Hartley David P. , Funsten Herbert O. , Spence Harlan E. , Baker Daniel N. 
Published: October 2019

AbstractNaturally occurring chorus emissions are a class of electromagnetic waves found in the space environments of the Earth and other magnetized planets. They play an essential role in accelerating high-energy electrons forming the hazardous radiation belt environment. Chorus typically occurs in two distinct frequency bands separated by a gap. The origin of this two-band structure remains a 50-year old question. Here we report, using NASA’s Van Allen Probe measurements, that banded chorus waves are commonly accompanied by two separate anisotropic electron components. Using numerical simulations, we show that the initially excited single-band chorus waves alter the electron distribution immediately via Landau resonance, and suppress the electron anisotropy at medium energies. This naturally divides the electron anisotropy into a low and a high energy components which excite the upper-band and lower-band chorus waves, respectively. This mechanism may also apply to the generation of chorus waves in other magnetized planetary magnetospheres.
Kisspeptin/Neurokinin B/Dynorphin (KNDy) cells as integrators of diverse internal and external cues: evidence from viral-based monosynaptic tract-tracing in mice
Authors: Moore Aleisha M. , Coolen Lique M. , Lehman Michael N. 
Published: October 2019

AbstractNeurons in the hypothalamic arcuate nucleus (ARC) that co-express kisspeptin, neurokinin B and dynorphin (KNDy cells) are essential for mammalian reproduction as key regulators of gonadotropin-releasing hormone (GnRH) secretion. Although multiple endogenous and exogenous signals act indirectly via KNDy neurons to regulate GnRH, the identity of upstream neurons that provide synaptic input to this subpopulation is unclear. We used rabies-mediated tract-tracing in transgenic Kiss1-Cre mice combined with whole-brain optical clearing and multiple-label immunofluorescence to create a comprehensive and quantitative brain-wide map of neurons providing monosynaptic input to KNDy cells, as well as identify the estrogen receptor content and peptidergic phenotype of afferents. Over 90% of monosynaptic input to KNDy neurons originated from hypothalamic nuclei in both male and female mice. The greatest input arose from non-KNDy ARC neurons, including proopiomelanocortin-expressing cells. Significant female-dominant sex differences in afferent input were detected from estrogen-sensitive hypothalamic nuclei critical for reproductive endocrine function and sexual behavior in mice, indicating KNDy cells may provide a unique site for the coordination of sex-specific behavior and gonadotropin release. These data provide key insight into the structural framework underlying the ability of KNDy neurons to integrate endogenous and environmental signals important for the regulation of reproductive function.
The freezing Rènyi quantum discord
Authors: Li Xiao-Yu , Zhu Qin-Sheng , Zhu Ming-Zheng , Wu Hao , Wu Shao-Yi , Zhu Min-Chuan 
Published: October 2019

AbstractAs a universal quantum character of quantum correlation, the freezing phenomenon is researched by geometry and quantum discord methods, respectively. In this paper, the properties of Rènyi discord is studied for two independent Dimer System coupled to two correlated Fermi-spin environments under the non-Markovian condition. We further demonstrate that the freezing behaviors still exist for Rènyi discord and study the effects of different parameters on this behaviors.
Sperm function in vitro and fertility after antibiotic-free, hypothermic storage of liquid preserved boar semen
Authors: Waberski Dagmar , Luther Anne-Marie , Grünther Benita , Jäkel Helen , Henning Heiko , Vogel Charlotte , Peralta Wolfgang , Weitze Karl Fritz 
Published: October 2019

AbstractThe role of antibiotics (AB) in semen extenders as a potential contribution to the global antimicrobial resistance threat is emerging. Here, we establish an AB-free hypothermic preservation strategy for boar semen and investigate its impact on sperm function, microbial load and fertility after artificial insemination (AI). Spermatozoa (12 boars) preserved in AB-free AndroStar Premium extender at 5 °C maintained high motility, membrane integrity, and a low DNA-fragmentation index throughout 72 h storage and results did not significantly differ from controls stored at 17 °C in extender containing AB (p = 0.072). Likewise, kinetic response of spermatoza to the capacitation stimulus bicarbonate during 180 min incubation in Tyrode’s medium did not differ from 17 °C-controls. In a competitive sperm oviduct binding assay, binding indices did not differ between semen stored for 72 h AB-free at 5 °C and 17 °C-controls (n = 6 boars). Bacterial load < 103 CFU/ml after 72 h was measured in 88.9% of samples stored at 5 °C AB-free compared to 97.2% in 17 °C-controls (n = 36 semen pools, 23 boars). Fertility traits of 817 females did not differ significantly between the two semen groups (p > 0.05). In conclusion, a hypothermic semen preservation strategy is presented which offers antibiotic-free storage of boar semen doses.