bluuebunny/crossref_metadata_2025_split huggingface dataset:
EGFR and HER3 expression in circulating tumor cells and tumor tissue from non-small cell lung cancer patients
Authors: Scharpenseel Heather , Hanssen Annkathrin , Loges Sonja , Mohme Malte , Bernreuther Christian , Peine Sven , Lamszus Katrin , Goy Yvonne , Petersen Cordula , Westphal Manfred , Glatzel Markus , Riethdorf Sabine , Pantel Klaus , Wikman Harriet 
Published: May 2019

AbstractAlthough clinically relevant, the detection rates of EpCAM positive CTCs in non-small cell lung cancer (NSCLC) are surprisingly low. To find new clinically informative markers for CTC detection in NSCLC, the expression of EGFR and HER3 was first analyzed in NSCLC tissue (n = 148). A positive EGFR and HER3 staining was observed in 52.3% and 82.7% of the primary tumors, and in 62.7% and 91.2% of brain metastases, respectively. Only 3.0% of the brain metastases samples were negative for both HER3 and EGFR proteins, indicating that the majority of metastases express these ERBB proteins, which were therefore chosen for CTC enrichment using magnetic cell-separation. Enrichment based on either EGFR or HER3 detected CTCs in 37.8% of the patients, while the combination of EGFR/HER3 enrichment with the EpCAM-based CellSearch technique detected a significantly higher number of 66.7% CTC-positive patients (Cohen’s kappa = −0.280) which underlines the existence of different CTC subpopulations in NSCLC. The malignant origin of keratin-positive/CD45-negative CTC clusters and single CTCs detected after EGFR/HER3 based enrichment was documented by the detection of NSCLC-associated mutations. In conclusion, EGFR and HER3 expression in metastasized NSCLC patients have considerable value for CTC isolation plus multiple markers can provide a novel liquid biopsy approach.
Modelling Dunes from Lençóis Maranhenses National Park (Brazil): Largest dune field in South America
Authors: dos Santos André Luís Silva , Borges Hélder Pereira , Silva Junior Celso Henrique Leite , Piedade Junior Raimundo Nonato , da Silva Bezerra Denilson 
Published: May 2019

AbstractThis paper presents a digital elevation model (DEM) of the dunes found in the Lençóis Maranhenses National Park, an environmental protection area located in the Maranhão state (Brazil). The DEM supports the modeling studies of sand-dune evolution using multi-temporal satellite images and ground truth data, obtained through the post-processed kinematic Global Navigation Satellite System (GNSS) positioning. The study area is located at the border of three major neotropical ecosystems: the Amazonia, Caatinga, and Brazilian savanna. It is located in the northeastern state of Maranhão and encompasses the largest dune fields in the country. Wide shrubby areas (restingas, in Portuguese), lakes, mangroves, and a multitude off reshwater lagoons compose the park’s natural environments. The objective of the present study is to create an DEM that can evidence the complex dynamics of dune formation in the study area with use of GNSS. Geodetic techniques and precision mapping were employed to monitor the short-term coastal dynamics. The use of GNSS receivers is justified by the difficulty of mapping the dune’s features using conventional methods such as theodolite, level, and total station systems, due to their high cost, time restriction sand low data precision. Surface surveys were carried out annually between December 2015 and January 2017 to create a DEM. The study results reveal that the area has a negative volumetric balance of erosion and a preferential direction of sediment transport by wind, which may justify the pattern of advancement and retraction observed in the dunes of the studied area.
Comprehensive and Systematic Analysis of Gene Expression Patterns Associated with Body Mass Index
Authors: Joseph Paule V. , Jaime-Lara Rosario B. , Wang Yupeng , Xiang Lichen , Henderson Wendy A. 
Published: May 2019

AbstractBoth genetic and environmental factors are suggested to influence overweight and obesity risks. Although individual loci and genes have been frequently shown to be associated with body mass index (BMI), the overall interaction of these genes and their role in BMI remains underexplored. Data were collected in 90 healthy, predominately Caucasian participants (51% female) with a mean age of 26.00 ± 9.02 years. Whole blood samples were assayed by Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. We integrated and analyzed the clinical and microarray gene expression data from those individuals to understand various systematic gene expression patterns underlying BMI. Conventional differential expression analysis identified seven genes RBM20, SEPT12, AX748233, SLC30A3, WTIP, CASP10, and OR12D3 associated with BMI. Weight gene co-expression network analysis among 4,647 expressed genes identified two gene modules associated with BMI. These two modules, with different extents of gene connectivity, are enriched for catabolic and muscle system processes respectively, and tend to be regulated by zinc finger transcription factors. A total of 246 hub genes were converted to non-hub genes, and 286 non-hub genes were converted to hub genes between normal and overweight individuals, revealing the network dynamics underlying BMI. A total of 28 three-way gene interactions were identified, suggesting the existence of high-order gene expression patterns underlying BMI. Our study demonstrated a variety of systematic gene expression patterns associated with BMI and thus provided novel understanding regarding the genetic factors for overweight and obesity risks on system levels.
Coronary Heart Disease and ABO Blood Group in Diabetic Women: A Case-Control Study
Authors: langari Seyyed Hasan , Bahar Adele , Asadian Leila , Abediankenai Saeid , Namazi Seyed Shojaeddin , Kashi Zahra 
Published: May 2019

AbstractNumerous investigations conducted in general population have reported that certain ABO blood group may increase the risk of coronary heart disease (CHD). However, this association has not been yet well established and even is less clear in diabetic patients. Considering that women with type 2 diabetes mellitus (T2DM) are at greater risk to develop CHD and have higher cardiovascular mortality, this study aimed to evaluate the association between CHD and ABO blood group in women with T2DM. A case control study of eight hundred eighty-one (881) diabetic women was enrolled in this study. Among them, two hundred thirty eight (238) patients were identified to have CHD (CHD+) and two hundred eighty two (282) of them were identified without CHD but matched with the first group for other CHD risk factors (CHD−). ABO blood type (A, B, AB, O, and Rhesus factor) for both groups were determined. To compare the magnitude of the correlation between various blood groups with CHD development, odd ratios (OR) with 95% confidence intervals (CI) was calculated. Our results demonstrates that the percentage of AB blood group was significantly higher in the diabetic women with concurrent CHD than in those without CHD [30 (12.7%) vs. 13 (4.6%), Odd ratio: 2.9 (95%CI: 1.5–5.7), P = 0.001]. The results of the present study clearly demonstrate that the AB blood group has a higher odd ratio for the development of CHD and can be considered as a risk factor for the development of CHD in females with T2DM. More comprehensive studies are required to confirm these results.
Single crossover-mediated targeted nucleotide substitution and knock-in strategies with CRISPR/Cas9 system in the rice blast fungus
Authors: Yamato Tohru , Handa Ai , Arazoe Takayuki , Kuroki Misa , Nozaka Akihito , Kamakura Takashi , Ohsato Shuichi , Arie Tsutomu , Kuwata Shigeru 
Published: May 2019

AbstractClustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9)-mediated genome editing has become a promising approach for efficient and versatile genetic engineering in various organisms; however, simple and precise nucleotide modification methods in filamentous fungi have been restricted to double crossover type homologous recombination (HR). In this study, we developed a novel genome editing strategy via single crossover-mediated HR in the model filamentous fungus Pyricularia (Magnaporthe) oryzae. This method includes the CRISPR/Cas9 system and a donor vector harboring a single homology arm with point mutations at the CRISPR/Cas9 cleavage site. Using this strategy, we demonstrated highly efficient and freely programmable base substitutions within the desired genomic locus, and target gene disrupted mutants were also obtained via a shortened (100–1000 bp) single homology arm. We further demonstrated that this method allowed a one-step GFP gene knock-in at the C-terminus of the targeted gene. Since the genomic recombination does not require an intact protospacer-adjacent motif within the donor construct and any additional modifications of host components, this method can be used in various filamentous fungi for CRISPR/Cas9-based basic and applied biological analyses.
A novel, rapid, seedless, in situ synthesis method of shape and size controllable gold nanoparticles using phosphates
Authors: Liu Kangze , He Zhonglei , Curtin James F. , Byrne Hugh J. , Tian Furong 
Published: May 2019

AbstractWe hereby report a novel synthesis method of size and shape controllable gold nanoparticles that is rapid, in situ and seedless. Unlike most currently employed size and shape controllable synthesis methods, it takes place in a single step under room temperature within ~15 minutes. While mixtures of gold nanospheres around 70 nm and gold nanoplates with width ranging from 100 nm to 1000 nm can be synthesized in about 15 minutes by standard synthesis method using N-2-hydroxyethylpiperazine-N-2-ethanesulphonic acid (HEPES) to reduce Au(III), gold nanoflowers or mixtures of smaller gold nanospheres and nanoplates can be synthesized with the addition of disodium phosphate (Na2HPO4) or monosodium phosphate (NaH2PO4), respectively. Increasing the concentration of phosphate added significantly reduces the formation time of gold nanoparticles to seconds. By increasing the molar ratio of Na2HPO4: HEPES and NaH2PO4: HEPES, the size of gold nanoflowers and gold nanoparticle mixtures can be tuned from ~60 nm down to 1 nm and from ~70 nm to ~2.5 nm, respectively. The systematic structural changes are accompanied by similarly systematic colour changes associated with shifting of the surface plasmon resonance. The proposed mechanism of the synthesis process is also presented.
Structural and functional characterization of the PDZ domain of the human phosphatase PTPN3 and its interaction with the human papillomavirus E6 oncoprotein
Authors: Genera Mariano , Samson Damien , Raynal Bertrand , Haouz Ahmed , Baron Bruno , Simenel Catherine , Guerois Raphael , Wolff Nicolas , Caillet-Saguy Célia 
Published: May 2019

AbstractThe human protein tyrosine phosphatase non-receptor type 3 (PTPN3) is a PDZ (PSD-95/Dlg/ZO-1) domain-containing phosphatase with a tumor-suppressive or a tumor-promoting role in many cancers. Interestingly, the high-risk genital human papillomavirus (HPV) types 16 and 18 target the PDZ domain of PTPN3. The presence of a PDZ binding motif (PBM) on E6 confers interaction with a number of different cellular PDZ domain-containing proteins and is a marker of high oncogenic potential. Here, we report the molecular basis of interaction between the PDZ domain of PTPN3 and the PBM of the HPV E6 protein. We combined biophysical, NMR and X-ray experiments to investigate the structural and functional properties of the PDZ domain of PTPN3. We showed that the C-terminal sequences from viral proteins encompassing a PBM interact with PTPN3-PDZ with similar affinities to the endogenous PTPN3 ligand MAP kinase p38γ. PBM binding stabilizes the PDZ domain of PTPN3. We solved the X-ray structure of the PDZ domain of PTPN3 in complex with the PBM of the HPV E6 protein. The crystal structure and the NMR chemical shift mapping of the PTPN3-PDZ/peptide complex allowed us to pinpoint the main structural determinants of recognition of the C-terminal sequence of the E6 protein and the long-range perturbations induced upon PBM binding.
Insulin prevents and reverts simvastatin-induced toxicity in C2C12 skeletal muscle cells
Authors: Sanvee Gerda M. , Bouitbir Jamal , Krähenbühl Stephan 
Published: May 2019

AbstractSimvastatin is an inhibitor of the 3-hydroxy-3-methylglutaryl-CoA reductase used for decreasing low density lipoprotein (LDL)-cholesterol in patients. It is well-tolerated but can cause myopathy. Our aims were to enlarge our knowledge regarding mechanisms and effects of insulin on simvastatin-associated myotoxicity in C2C12 myotubes. Simvastatin (10 µM) reduced membrane integrity and ATP content in myotubes treated for 24 hours, which could be prevented and partially reversed concentration- and time-dependently by insulin. Furthermore, simvastatin impaired the phosphorylation of Akt (Protein Kinase B) mainly at Ser473 and less at Thr308, indicating impaired activity of the mammalian Target of Rapamycin Complex 2 (mTORC2). Impaired activation of Akt increased mRNA expression of the muscle atrophy F-Box (MAFbx), decreased activation of the mammalian Target of Rapamycin Complex 1 (mTORC1) and stimulated apoptosis by impairing the Ser9 phosphorylation of glycogen synthase kinase 3β. Decreased phosphorylation of Akt at both phosphorylation sites and of downstream substrates as well as apoptosis were prevented concentration-dependently by insulin. In addition, simvastatin caused accumulation of the insulin receptor β-chain in the endoplasmic reticulum (ER) and increased cleavage of procaspase-12, indicating ER stress. Insulin reduced the expression of the insulin receptor β-chain but increased procaspase-12 activation in the presence of simvastatin. In conclusion, simvastatin impaired activation of Akt Ser473 most likely as a consequence of reduced activity of mTORC2. Insulin could prevent the effects of simvastatin on the insulin signaling pathway and on apoptosis, but not on the endoplasmic reticulum (ER) stress induction.
Trends in Vegetation fires in South and Southeast Asian Countries
Authors: Vadrevu Krishna Prasad , Lasko Kristofer , Giglio Louis , Schroeder Wilfrid , Biswas Sumalika , Justice Chris 
Published: May 2019

AbstractWe assessed the fire trends from Moderate Resolution Imaging Spectroradiometer (MODIS) (2003–2016) and Visible Infrared Imaging Radiometer Suite (VIIRS) (2012–2016) in South/Southeast Asia (S/SEA) at a country level and vegetation types. We also quantified the fire frequencies, anomalies and climate drivers. MODIS data suggested India, Pakistan, Indonesia and Myanmar as having the most fires. Also, the VIIRS-detected fires were higher than MODIS (AQUA and TERRA) by a factor of 7 and 5 in S/SEA. Thirty percent of S/SEA had recurrent fires with the most in Laos, Cambodia, Thailand, and Myanmar. Statistically-significant increasing fire trends were found for India (p = 0.004), Cambodia (p = 0.001), and Vietnam (p = 0.050) whereas Timor Leste (p = 0.004) had a decreasing trend. An increasing trend in fire radiative power (FRP) were found for Cambodia (p = 0.005), India (0.039), and Pakistan (0.06) and declining trend in Afghanistan (0.041). Fire trends from VIIRS were not significant due to limited duration of data. In S/SEA, fires in croplands were equally frequent as in forests, with increasing fires in India, Pakistan, and Vietnam. Specific to climate drivers, precipitation could explain more variations in fires than the temperature with stronger correlations in Southeast Asia than South Asia. Our results on fire statistics including spatial geography, variations, frequencies, anomalies, trends, and climate drivers can be useful for fire management in S/SEA countries.
Red Blood Cell Membrane Bioengineered Zr-89 Labelled Hollow Mesoporous Silica Nanosphere for Overcoming Phagocytosis
Authors: Lee Jun Young , Vyas Chirag K. , Kim Gun Gyun , Choi Pyeong Seok , Hur Min Goo , Yang Seung Dae , Kong Young Bae , Lee Eun Je , Park Jeong Hoon 
Published: May 2019

AbstractBiomimetic nanoparticles (NPs) have been actively studied for their biological compatibility due to its distinguished abilities viz. long-term circulation, low toxicity, ease for surface modification, and its ability to avoid phagocytosis of NPs by macrophages. Coating the NPs with a variety of cell membranes bearing the immune control proteins increases drug efficacy while complementing the intrinsic advantages of the NPs. In this study, efforts were made to introduce oxophilic radiometal 89Zr with hollow mesoporous silica nanospheres (HMSNs) having abundant silanol groups and were bioengineered with red blood cell membrane (Rm) having cluster of differentiation 47 (CD47) protein to evaluate its long-term in vivo behavior. We were successful in demonstrating the increased in vivo stability of synthesized Rm-camouflaged, 89Zr-labelled HMSNs with the markedly reduced 89Zr release. Rm camouflaged 89Zr-HMSNs effectively accumulated in the tumor by avoiding phagocytosis of macrophages. In addition, re-injecting the Rm isolated using the blood of the same animal helped to overcome the immune barrier. This novel strategy can be applied extensively to identify the long-term in vivo behavior of nano-drugs while enhancing their biocompatibility.
Natural stibnite ore (Sb2S3) embedded in sulfur-doped carbon sheets: enhanced electrochemical properties as anode for sodium ions storage
Authors: Deng Mingxiang , Li Sijie , Hong Wanwan , Jiang Yunling , Xu Wei , Shuai Honglei , Li Hui , Wang Wenlei , Hou Hongshuai , Ji Xiaobo 
Published: May 2019

Antimony sulfide (Sb2S3) has drawn widespread attention as an ideal candidate anode material for sodium-ion batteries (SIBs) due to its high specific capacity of 946 mA h g−1 in conversion and alloy reactions.
Plastidic glucose-6-phosphate dehydrogenases are regulated to maintain activity in the light
Authors: Preiser Alyssa L. , Fisher Nicholas , Banerjee Aparajita , Sharkey Thomas D. 
Published: May 2019

Abstract
Glucose-6-phosphate dehydrogenase (G6PDH) can initiate the glucose-6-phosphate (G6P) shunt around the Calvin–Benson cycle. To understand the regulation of flux through this pathway, we have characterized the biochemical parameters and redox regulation of the three functional plastidic isoforms of Arabidopsis G6PDH. When purified, recombinant proteins were measured, all three exhibited significant substrate inhibition by G6P but not NADP+, making the determination of enzyme kinetic parameters complex. We found that the half-saturation concentration of G6PDH isoform 1 is increased under reducing conditions. The other two isoforms exhibit less redox regulation, however, isoform 2 is strongly inhibited by NADPH. Redox regulation of G6PDH1 can be partially reversed by hydrogen peroxide or protected against by the presence of its substrate, G6P. Overall, our results support the conclusion that G6PDH can have significant activity throughout the day and can be dynamically regulated to allow or prevent flux through the glucose-6-phosphate shunt.
Cost‐effective inductive power transfer charging system for electric bicycles with variable charging current using primary‐side detuned series‐series topology
Authors: Chen Yang , Li Mingxuan , Kou Zhihao , He Zhengyou , Mai Ruikun 
Published: May 2019

Due to the convenience and long travelling distance, electric bicycles (EBs) are becoming more and more popular. Charging EBs with inductive power transfer (IPT) technology can free people from wearisome plugging actions and avoid electric shock hazards. However, the cost, including component cost and control cost, is a big challenge for the extensive promotion of the IPT charging systems (IPTCSs) for EBs. This article proposes an IPTCS using primary‐side detuned series‐series (SS) compensation topology, cancelling control scheme and reducing the component cost. With the proposed parametric design method, the IPTCS can operate with variable charging current according to the variable equivalent resistance of a battery. Besides, the inductive input impedance is guaranteed to achieve soft switching for the high‐frequency inverter. An experimental setup is constructed to validate the proposed method. The performance shows that the charging current decreases from 1.8 to 0.1 A, and charging voltage increase from 42.23 to 54.01 V during the whole charging process, where the maximum efficiency of the IPTCS can reach up to 92.8%. The results demonstrate the proposed IPTCS is a cost‐effective and potential solution for charging EBs.
Matrix wreath products of algebras and embedding theorems
Authors: Alahmadi Adel , Alsulami Hamed , Jain S. , Zelmanov Efim 
Published: May 2019

We introduce a new construction of matrix wreath products of algebras that is similar to wreath products of groups. We then use it to prove embedding theorems for Jacobson radical, nil, and primitive algebras. In §6, we construct finitely generated nil algebras of arbitrary Gelfand-Kirillov dimension

≥
8

\geq 8

over a countable field which answers a question from [New trends in noncommutative algebra, Amer. Math. Soc., Providence, RI, 2012, pp. 41–52].
Longitudinal Growth Trajectories of Written Syntactic Complexity: The Case of Turkish Learners in an Intensive English Program
Authors: Polat Nihat , Mahalingappa Laura , Mancilla Rae L 
Published: May 2019

Abstract
This longitudinal study examined growth patterns of written syntactic complexity of Turkish learners of English. Using a nonexperimental corpus of 852 writing samples by 284 English as a foreign language (EFL) learners over three semesters, the study addressed the following questions: which indices of syntactic complexity characterize the writing of EFL learners most saliently at elementary, pre-intermediate, and intermediate levels? How do levels of the indices of syntactic complexity change over time as learners progress from elementary to pre-intermediate and intermediate levels? Results suggested that lower proficiency level (levels 1 and 2) writers produced similar patterns of written syntactic complexity (reliance on phrasal coordination), while at proficiency level 3, they demonstrated constructions that were more complex and of greater variety. Findings revealed that over time, learners experienced significant syntactic changes in multiple aspects of complexity; however, these changes were not necessarily straightforward or incremental across the three levels. These findings underscore issues of unpredictability and linearity in second language acquisition, while also cautioning us about the role of intensity of classroom instruction in L2 development.