bluuebunny/crossref_metadata_2025_split huggingface dataset:
Modern botanical analogue of endangered Yak (Bos mutus) dung from India: Plausible linkage with living and extinct megaherbivores
Authors: Basumatary Sadhan K. , Singh Hukam , McDonald H. Gregory , Tripathi Swati , Pokharia Anil K. 
Published: August 2018

AbstractThe study present to document the micro and macrobotanical remain on wild Yak dung to understand the diet, habitat, and ecology in relation to determining possible ecological relationships with extant and extinct megaherbivores. Grasses are the primary diet of the yak as indicated by the abundance of grass pollen and phytoliths, though it is obvious. The other associates non-arboreal and arboreal taxa namely, Cyperacaeae, Rosaceae, Chenopodiaceae,Artemisia, Prunus, andRhododendronare also important dietary plants for their survival. The observation of plant macrobotanical remains especially the vegetative part and seed of the grasses and Cyperaceae also indicates good agreement with the palynodata. The documented micro and macrobotanical data is indicative of both Alpine meadow and steppe vegetation under cold and dry climate which exactly reflected the current vegetation composition and climate in the region. The recovery ofBotryococcus, Arcella, and diatom was marked though in trace values and suggestive of the perennial water system in the region which incorporated through the ingestion of water. Energy dispersive spectroscopy analysis marked that the element contained in dung samples has variation in relation to the summer and winter which might be the availability of the food plants and vegetation. This generated multiproxy data serves as a strong supplementary data for modern pollen and vegetation relationship based on surface soil samples in the region. The recorded multiproxy data could be useful to interpret the coprolites of herbivorous fauna in relation to the palaeodietary and paleoecology in the region and to correlate with other mega herbivores in a global context.
Are these cardiomyocytes? Protocol development reveals impact of sample preparation on the accuracy of identifying cardiomyocytes by flow cytometry
Authors: Waas Matthew , Weerasekera Ranjuna , Kropp Erin M. , Romero-Tejeda Marisol , Poon Ellen , Boheler Kenneth R. , Burridge Paul W. , Gundry Rebekah L. 
Published: August 2018

SummaryModern differentiation protocols enable efficient, yet imperfect, differentiation of human pluripotent stem cells into cardiomyocytes (hPSC-CM). As the number of laboratories and studies implementing this technology expands, the accurate assessment of cell identity in differentiation cultures is paramount to well-defined studies that can be replicated among laboratories. While flow cytometry is apt for routine assessment, a standardized protocol for assessing cardiomyocyte identity in hPSC-CM cultures has not yet been established. To address this gap, the current study leveraged targeted mass spectrometry to confirm the presence of troponin proteins in hPSC-CM and systematically evaluated multiple anti-troponin antibodies and sample preparation protocols for their suitability in assessing cardiomyocyte identity. Results demonstrate challenges of interpreting data generated by published methods and informed the development of a robust protocol for routine assessment of hPSC-CM. Overall, the new data, workflow for evaluating fit-for-purpose use of antibodies, and standardized protocol described here should benefit investigators new to this field as well as those with expertise in hPSC-CM differentiation.
ChIP-seq meta-analysis yields high quality training sets for enhancer classification
Authors: Imrichova Hana , Aerts Stein 
Published: August 2018

AbstractGenome-wide prediction of enhancers depends on high-quality positive and negative training sets. The use of ChIP-seq peaks as positive training data can be problematic due to high degrees of indirectly bound regions, and often poor overlap between experimental conditions.Here we explore meta-analysis of ChIP-seq data to generate high-quality training data for enhancer modeling. Our method is based on rank aggregation and identifies a core set of directly bound regions per transcription factor, exploiting between five and twenty ChIP-seq data sets per factor. We applied this method to six different transcription factors, namely TP53, REST, SOX2, GRHL2, HIF1A and PPARG. Sequence analysis and modeling of recurrently bound enhancers yielded distinct enhancer features for the different factors, whereby binding sites of REST and TP53 are strongly determined by their motif; binding of GRHL2 and SOX2 is determined by nucleosome positioning; and binding of PPARG and HIF1A depends on other transcription factors. In conclusion, meta-analysis of ChIP-seq peaks, and centering on motifs, allowed discovering new properties of transcription factor binding.
Parallel evolution of frog antimicrobial peptides produces identical conformations but subtly distinct membrane and antibacterial activities
Authors: Manzo Giorgia , Ferguson Philip M. , Hind Charlotte , Clifford Melanie , Gustilo V. Benjamin , Ali Hind , Bansal Sukhvinder S. , Bui Tam T. , Drake Alex F. , Atkinson R. Andrew , Sutton Mark J. , Lorenz Christian D. , Phoenix David A. , Mason A. James 
Published: August 2018

ABSTRACTFrogs such as Rana temporaria and Litoria aurea secrete numerous closely related antimicrobial peptides (AMPs) as an effective chemical dermal defence. Despite the high similarity in physical properties and preference for adopting secondary amphipathic, α-helix conformations in membrane mimicking milieu, their spectrum of activity and potency often varies considerably. Damage or penetration of the bacterial plasma membrane is considered essential for AMP activity and hence distinguishing apparently similar AMPs according to their behaviour in, and effects on, model membranes will inform understanding of species specific effective antimicrobial mechanisms. Here we use a combination of molecular dynamics simulations, circular dichroism and patch-clamp to investigate the basis for differing anti-bacterial activities in representative AMPs from each species; temporin L and aurein 2.5. Despite adopting near identical, α-helix conformations in the steady-state in a variety of membrane models, these two AMPs can be distinguished both in vitro and in silico based on their dynamic interactions with model membranes; the greater conformational flexibility and the higher amplitude channel conductance induced offers a rationale for the greater potency and broader spectrum of activity of temporin L over aurein 2.5. Specific contributions from individual residues are identified that define the mechanisms of action of each AMP. Our findings suggest AMPs in frogs are examples of parallel evolution whose utility is based on apparently similar but subtly distinct mechanisms of action.
UBE2G1 Governs the Destruction of Cereblon Neomorphic Substrates
Authors: Lu Gang , Weng Stephanie , Matyskiela Mary , Zheng Xinde , Fang Wei , Wood Scott , Surka Christine , Mizukoshi Reina , Lu Chin-Chun , Mendy Derek , Jang In Sock , Wang Kai , Marella Mathieu , Couto Suzana , Cathers Brian , Carmichael James , Chamberlain Philip , Rolfe Mark 
Published: August 2018

AbstractThe immunomodulatory drugs (IMiDs) thalidomide, lenalidomide, and pomalidomide as well as the novel cereblon modulating agents (CMs) including CC-122, CC-220 and cereblon-based proteolysis-targeting chimaeras (PROTACs) repurpose the Cul4-RBX1-DDB1-CRBN (CRL4CRBN) E3 ubiquitin ligase complex to induce the degradation of specific neomorphic substrates via polyubiquitination in conjunction with an E1 ubiquitin-activating enzyme and E2 ubiquitin-conjugating enzymes, which have until now remained elusive. Here we show that the ubiquitin-conjugating enzymes UBE2G1 and UBE2D3 cooperatively promote the polyubiquitination of CRL4CRBN neomorphic substrates in a cereblon- and CM-dependent manner via a sequential ubiquitination mechanism: UBE2D3 transforms the neomorphic substrates into mono-ubiquitinated forms, upon which UBE2G1 catalyzes K48-linked polyubiquitin chain extension. Blockade of UBE2G1 diminishes the ubiquitination and degradation of neomorphic substrates, and consequent antitumor activities elicited by all tested CMs. For example, UBE2G1 inactivation significantly attenuated the degradation of myeloma survival factors IKZF1 and IKZF3 induced by lenalidomide and pomalidomide, hence conferring drug resistance. UBE2G1-deficient myeloma cells, however, remained sensitive to a more potent IKZF1/3 degrader CC-220. Collectively, these findings suggest that loss of UBE2G1 activity might be a resistance mechanism to drugs that hijack the CRL4CRBN to eliminate disease-driving proteins, and that this resistance mechanism can be overcome by next-generation CMs that destroy the same targeted protein more effectively.
Brazilian infant dairy foods: mineral content and daily intake contribution
Authors: Pimentel Luana S. , Moraes Jeremias , Luna Aderval S. , Barros Diego B. , Pimentel Tatiana C. , Guimarães Jonas T. , Silva Hugo L.A. , Balthazar Celso F. , Esmerino Erick A. , Freitas Mônica Q. , Ranadheera C.S. , Silva Marcia C. , Quitério Simone L. , Raices Renata S.L. , Cruz Adriano G. 
Published: August 2018

Purpose
The purpose of this paper is to investigate the mineral content of seven Brazilian infant dairy product categories (petit Suisse cheeses, fermented milks, yogurts, fermented dairy beverage, dairy dessert, Requeijão cremoso spreadable cheese and UHT dairy beverages) and estimate their contribution to daily intake.

Design/methodology/approach
The composition of major (Ca, K, Mg, and Na) and trace (Pb, Cd, Cu and Mn) minerals was determined using Inductively Coupled Plasma Optical Emission Spectrometry. Furthermore, a comparative analysis of the mineral levels with the Reference Daily Intake (RDI) at different child development stages was carried out.

Findings
High Ca levels were observed in petit Suisse (3.44±1.66 mg g−1), dairy dessert (3.88±0.02 mg g−1) and Requeijão cremoso (4.14±0.07 mg g−1). Dairy dessert presented the highest K level (2.57±0.07 mg g−1), while the Requeijão cremoso presented the highest Na content (4.78±0.10 mg g−1), and both products had the highest Mg contents (238.55±16.27 and 197.39±5.18 µg g−1, respectively). Trace elements (Cd, Cu, Mn and Pb) were below the limit of detection for all commercial dairy foods. Among food products analyzed, petit Suisse cheese and dairy dessert can be considered good sources of calcium, while Requeijão cremoso is high in both calcium and sodium.

Originality/value
This study is the first to analyze the mineral levels of several Brazilian infant dairy foods and the daily intake contribution during important child development stages. These findings provide valuable guidance for researchers and practitioners trying to develop healthy and nutritious dairy products for infants and children.
Attributes determining consumer preference for organic rice in Bangkok, Thailand
Authors: Sakolwitayanon Hatairat , Soni Peeyush , Damien Jourdain 
Published: August 2018

Purpose
The purpose of this paper is to explore key attributes of organic rice that consumers use in the process of choosing organic rice, and to segment organic rice market in Bangkok. Moreover, the study tends to identify the best clustering techniques, between latent class cluster analysis (LCCA) and traditional cluster analysis (CA), for precise segmentation.

Design/methodology/approach
Best–worst scaling (BWS) method was applied to measure the level of relative importance of organic rice attributes. Then, LCCA and CA techniques were applied to recognize market segmentation. Finally, homogeneity and heterogeneity of the resulting clusters were determined to compare performance of the two clustering techniques.

Findings
The LCCA technique was identified better than the CA in classification of consumers. According to LCCA solution, the organic rice market in Bangkok (Thailand) consisted of six distinct clusters, which can be grouped into three categories based on consumers’ profile. Organic rice consumer categories were identified as “Art of eating” and “Superior quality seeker” clusters focusing on special features and quality of the organic rice; consumer category “Basic concern” cluster heavily relied on organic certification logo and manufacturing information; and other consumer categories were “Price driven,” “Eyes on price” and “Thorough explorer” clusters.

Originality/value
This study first applies BWS score to examine consumers’ preference for organic rice attributes and segments market, providing results for practical use for retailers, producers and marketers.
Knowledge transfer in a start-up craft brewery
Authors: Cardoni Andrea , Dumay John , Palmaccio Matteo , Celenza Domenico 
Published: August 2018

PurposeThe purpose of this paper is to explore the role of the entrepreneur in the knowledge transfer (KT) process of a start-up enterprise and the ways that role should change during the development phase to ensure mid-term business survival and growth.Design/methodology/approachAn in-depth, qualitative case study of Birra Flea, an Italian Craft Brewery, is presented and analysed using Liyanageet al.’s (2009) framework to identify the key components of the KT process, including relevant knowledge, key actors, transfer steps and the criteria for assessing its effectiveness and success.FindingsThe entrepreneur played a fundamental and crucial role in the start-up process, acting as a selective and passionate broker for the KT process. As Birra Flea matures and moves into the development phase, the role of the entrepreneur as KT’s champion needs to be integrated and distributed throughout the organisation, with the entrepreneur serving as a performance controller.Research limitations/implicationsThis study enriches the knowledge management literature by applying a framework designed to provide a general description of KT, with some modifications, to a single case study to demonstrate its effectiveness in differentiating types of knowledge and outlining how KT can be configured to support essential business functions in an SME.Practical implicationsThe analysis systematises the KT mechanisms that govern the start-up phase of an award-winning SME, with suggestions for how to manage KT during the development phase. Seldom are practitioners given insight into the mechanics of a successful SME start-up; this analysis serves as a practical guide for those wishing to implement effective KT strategies to emulate Birra Flea’s success.Originality/valueThe world’s economy thrives on SMEs, yet many fail as start-ups before they even have a chance to reach the development phase, presenting a motivation to study the early stages of SMEs. This study addresses that gap with an in-depth theoretical analysis of successful, effective KT processes in an SME, along with practical implications to enhance the knowledge, experience and skills of the actors that sustain these vital economic enterprises.
When hierarchy becomes collaborative
Authors: Rice Rebecca M. 
Published: August 2018

Purpose
The purpose of this paper is to expand understandings of interorganizational collaboration among high reliability organizations (HROs). It proposes that HROs face unique needs for relationship building, pre-planning, and retrospective sensemaking that do not fit within prior models of collaboration. For HROs, definitions of collaboration vary contextually based on needs that arise during emergency situations. HROs have a need for both hierarchical structure and collaborative processes and use collaboration as a sensemaking frame that allows practitioners to attend to both needs.

Design/methodology/approach
The paper uses a case study from an ongoing ethnographic study of an emergency response collaboration. The paper uses open-ended interviews about collaboration with all key members of the incident response hierarchy, and participant observation of collaboration before, during and after a key emergency incident.

Findings
The paper proposes a new framework for HRO collaboration: that collaboration is a sensemaking frame for HROs used to make sense of individual actions, that HRO collaboration is more complex during pre-planning and focused on individual decision making during incidents, and that members can communicatively make sense of the need for hierarchy and collaborative action by defining these needs contextually.

Research limitations/implications
The paper uses an in-depth case study of an incident to explore this collaborative framework; therefore, researchers are encouraged to test this framework in additional high reliability collaborative contexts.

Practical implications
The paper includes implications for best communicative practices to recognize the need to be both hierarchical and flexible in high reliability organizing.

Originality/value
This paper fulfills a need to expand collaboration literature beyond idealized and egalitarian definitions, in order to understand how practitioners use communication to understand their actions as collaborative, especially in organizations that also require hierarchy and individual actions. This case study suggests that collaboration as a sensemaking frame creates collaborative advantages for HROs, but can also limit sensemaking about incident management.
Cannabis use as harm reduction in the Eastern Caribbean
Authors: Day Marcus 
Published: August 2018

Purpose
The purpose of this paper is to present evidence of the therapeutic value of cannabis as a harm reduction intervention with people who smoke crack cocaine.

Design/methodology/approach
A desk study of published peer-reviewed material supporting the use of cannabis as therapeutic in mitigating some of the harms associated with crack cocaine smoking.

Findings
The use of cannabis as a harm reduction strategy for crack cocaine use has been commented on in the scientific literature since the 1980s. The officially scheduling of cannabis as having no medicinal value hampered further study despite the reporting of positive findings and numerous calls for more research.

Practical implications
There are currently no approved pharmaceutical substitutions for crack cocaine. Cannabis has shown itself effective in mitigating harms for 30–40 per cent of people. Cannabis is inexpensive and readily available and should be allowed for those people who want to use it.

Originality/value
Poly drug use is often framed in a negative context. In this paper, the author shows that with cannabis and crack, the poly drug use is actually a valid harm reduction strategy.
Lean talent management: a novel approach for increasing creativity in architectural design firms
Authors: Othman Ayman Ahmed Ezzat , Khalil Mohamed Hesham Madbouly 
Published: August 2018

Purpose
Architecture is classified as one of the creative industries worldwide. However, it is plagued with a number of problems that confine its creativity towards developing innovative solutions that fulfil society needs. One of these pressing problems is the lack of creativity due to the non-utilisation of talented architects. The purpose of this paper is to investigate the role of lean talent management (LTM) as a novel approach to increase creativity in architectural design firms (ADFs).

Design/methodology/approach
In order to achieve the above-mentioned aim, a mixed qualitative and quantitative methodology is designed to accomplish three objectives. First, reviewing literature to investigate the concepts of creative industries, motivation, demotivation, talent management (TM), talents utilisation (TU), lean thinking (LT) and lean innovation (LI). Second, presenting and analysing five case studies to explore the integration between TM, LT and LI in real-life context to overcome barriers of creativity. Finally, analysing the results of a survey questionnaire conducted with a representative sample of Egyptian ADFs to evaluate their perception and application of TM as a facilitator for creativity.

Findings
The five barriers of creativity encompassed lack of motivation in ADFs and demotivation and lack of TU in ADFs and Architecture, Engineering and Construction (AEC) industry. TM facilitated motivation and eliminated six demotivating factors. The seventh factor was eliminated through LT. In AEC industry, TU was achieved through LI with five considerations. In ADFs, TU can be achieved through LTM. However, two additional barriers have been identified for further investigation. Egyptian ADFs failed increasing creativity with TM solely. Hence, a LTM framework is required.

Research limitations/implications
Although the study focussed on architecture as a case for creative industries and targeted Egyptian ADFs, the application of LTM is to be applied at any industry that is talent-based although barriers related to the design process could vary.

Originality/value
This paper presents an innovative approach through discussing the integration of lean concepts into TM towards increasing creativity in ADFs. This ideology has received scant attention in construction literature and is the first of its kind in the architectural field. The proposed ideas represent a synthesis that is novel and creative in thought and adds value to the knowledge in a manner that has not previously occurred.
HRM practices and the multifaceted nature of organization performance
Authors: Taamneh Abdallah , Alsaad Abdallah Khalaf , Elrehail Hamzah 
Published: August 2018

PurposeThe purpose of this paper is to investigate the impact of human resource management (HRM) practices on the performance of Jordanian banks as determined by using the balanced scorecard (BSC) performance measurement system and by testing the effect of organizational citizenship behavior (OCB) as a possible mediator variable.Design/methodology/approachA questionnaire was used to collect data from the study sample, which consisted of 230 managers working in various banks across Jordan. The study hypotheses were then tested using SPSS and AMOS software by applying structural equation models (SEMs).FindingsThe data set revealed that the use of HRM practices had a significant impact on both employee OCB and bank performance in all the four dimensions of the BSC (financial, customer satisfaction, internal processes, learning and growth). In addition, OCB was found to have a positive significant impact on organizational performance. Moreover, results indicated that OCB partially mediates the relationship between HRM practices and organizational performance.Originality/valueThe authors examine the impact HRM practices on the organization performance through the mediation role of OCB. The results obtained from this study extend the existing literature by providing evidences from non-western country such as Jordan. Based on the findings, the theoretical and practical implications of the study as well as limitations and suggestions for future studies are also discussed.