bluuebunny/crossref_metadata_2025_split huggingface dataset:
Abstract B063: Race-related LMO7 exon skipping enhances prostate tumor invasion
Authors: Al Abo Muthana , Jiang Alice , George Daniel J , Wang Zefeng , Patierno Steven R , Freedman Jennifer A 
Published: September 2020

Abstract
Prostate cancer (PCa) affects disproportionally African American (AA) men in comparison with white or Asian men. According to the Surveillance, Epidemiology, and End Results Program (SEER), the mortality from PCa in black men is 2-5 times higher than in white or Asian men. In addition to differences in social determinants of health, differences in biological mechanisms contribute to the PCa disparity. We reported previously results from a comparative analysis of the transcriptomes of PCa specimens from 20 AA and 15 white patients, identifying 1,188 differentially expressed genes. In addition to the race-related aggregate gene expression differences, we also identified 2,520 differential RNA splicing events between these tumor specimens from AA and white patients. Among the 2,520 events, we prioritized 25 events, which had also been reported by Tsai et. al., 2015 to be differentially expressed between tumor and normal breast, lung and liver samples in The Cancer Genome Atlas (TCGA). To elucidate the function of these 25 events, we used Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR associated protein 9 (Cas9) methodology to generate PCa cells expressing each of the race-related alternative RNA splice variants. To date, we have generated DU145 PCa cells that express the following exon skipping events: LIM Domain 7 (LMO7) exon 12, Integrin Subunit Alpha 6 (ITGA6) exon 27 and Actin Binding LIM Protein Family Member 3 (ABLIM3) exon 14. The genotype of these cells was confirmed by PCR. Preliminary proliferation analysis demonstrated that skipping of ITGA6 exon 27 slowed doubling time of the cells compared with the parental cells. Interestingly, the cells expressing the LMO7 exon 12 skip appear more mesenchymal-like. Preliminary invasion analysis suggests that LMO7 exon 12 skipping increases (~ 4 times) the ability of the cells to pass through a Matrigel matrix, suggesting an increased invasive and metastatic potential of these cells. In conclusion, we have identified race-related RNA splicing events in PCa that function in processes relevant to oncogenesis. Further analysis of the biological significance of these race-related RNA splicing events in vitro and in vivo is underway. Our approach to analyze RNA splicing in the context of PCa disparity has the potential to uncover novel biological mechanisms underlying this disparity and to discover novel therapeutic targets.
Citation Format: Muthana Al Abo, Alice Jiang, Daniel J George, Zefeng Wang, Steven R Patierno, Jennifer A Freedman. Race-related LMO7 exon skipping enhances prostate tumor invasion [abstract]. In: Proceedings of the Twelfth AACR Conference on the Science of Cancer Health Disparities in Racial/Ethnic Minorities and the Medically Underserved; 2019 Sep 20-23; San Francisco, CA. Philadelphia (PA): AACR; Cancer Epidemiol Biomarkers Prev 2020;29(6 Suppl_2):Abstract nr B063.
Abstract B071: PVT1 regulation of claudin expression in triple-negative breast cancer
Authors: Levine Fayola , Ogunwobi Olorunseun 
Published: September 2020

Abstract
Breast cancer (BC) is a heterogeneous disease that is classically driven by the estrogen receptor (ER), progesterone receptor (PR) and human epithelial growth factor receptor 2 (EGFR2/HER2) signaling pathways. Triple negative breast cancer (TNBC), is a lethal subtype of invasive BC tumors that are ER-, PR- and HER2-. A subtype of TNBC is claudin low (CL). Dysregulation of claudin proteins disrupts tight junctions, consequently inducing the epithelial-to-mesenchymal transition (EMT) in cancers. This leads to enhanced motility and metastasis. Patients with CL TNBC have worse prognosis than patients with other BC subtypes. African American (AA) women with TNBC account for almost 20% of all BC cases and premenopausal African American (AA) women are more likely to die from the disease. PVT1 is a long noncoding RNA (lncRNA) transcribed from the 8q24 genomic locus that has been implicated in multiple cancers including BC. Amplification of the 8q24 gene locus is a common event in many malignant diseases and is associated with poor survival rate among patients. Although previous research demonstrates a critical functional role which PVT1 plays in BC, the underlying molecular mechanisms of PVT1 in CL TNBC was previously unknown. We assessed PVT1 expression in BC, and we observed that PVT1 exons 4A, 4B, and 9 are significantly upregulated in MDA MB 231 cells (claudin low) and significantly downregulated in MDA MB 468 cells (claudin high), in comparison to T47D (ER+). We have confirmed that claudin expression, specifically claudins 1, 3, 4 and 7, are higher in MDA MB 468 and lower in MDA MB 231. When PVT1 exon 9 expression is silenced in the MDA MB 231 CL TNBC cell line, we observed a significant reduction in migration when compared to cells transfected with a control scramble siRNA, suggesting that PVT1 exon 9 may be regulating migration in CL TNBC. Further investigation could lead to an understanding of the molecular mechanisms by which PVT1 exon 9 regulates claudin expression and consequently clinical outcome in TNBC.
Citation Format: Fayola Levine, Olorunseun Ogunwobi. PVT1 regulation of claudin expression in triple-negative breast cancer [abstract]. In: Proceedings of the Twelfth AACR Conference on the Science of Cancer Health Disparities in Racial/Ethnic Minorities and the Medically Underserved; 2019 Sep 20-23; San Francisco, CA. Philadelphia (PA): AACR; Cancer Epidemiol Biomarkers Prev 2020;29(6 Suppl_2):Abstract nr B071.
Abstract B093: Upregulated and downregulated microRNA-signatures in thyroid cancer health disparities
Authors: Davis Ryan , Wongworawat Yan C , Perez Mia C , Santiago Krystal , Roy Saurav , Wang Charles , Simental Alfred A , Khan Salma 
Published: September 2020

Abstract
Filipino Americans (FA) are known to have a higher incidence, recurrence, and mortality of thyroid cancer (TC) than other Asians Americans or European Americans (EA). Understanding the molecular mechanisms that underpin the genetic defect in metabolism and their impact on thyroid cancer oncogenesis in FA are critical for addressing the health disparity. Mechanisms that link genetic defects and lifestyle factors to thyroid cancer progression have not been defined. Lifestyle and genetic variations among FA lead them to a higher risk of metabolic diseases but no study showed to elucidate the mechanism of thyroid health disparities cancer so far. Recent studies clearly demonstrate that miRNA plays an important role in carcinogenesis via targeting both oncogene and tumor suppressor gene and lifestyle changes may potentially alter miRNA signatures. Therefore, we hypothesize that miRNA expression contributes to tumor grade, prognosis, and thyroid cancer health disparities. We examined miRNA expression profiles in FA vs. EA. We analyzed miRNA-sequence (miRNA-seq) by next-generation sequencing (NGS). The pathways linked to miRNAs were analyzed by ingenuity pathway analysis (IPA) software. Correlations between disease staging to miRNA upregulation and downregulation were elucidated. Both upregulated and downregulated miRNAs were validated by quantitative real-time PCR (qPCR) using miRNA extracted from paraffin-embedded thyroid tissues. We found hundreds of upregulated and downregulated miRNA and piRNA from this study. We chose 10 miRNA down-regulated, and 10 up-regulated miRNA FA compared to EA TC patients (p&lt;0.05). Quantitative PCR (qPCR) was done to confirm our NGS data. Differential miRNA expressions between FA vs. EA patient tumor tissues were observed with advanced staging. Evolutionarily conserved miRNA clusters, which are known to initiate malignancy, were shown to be upregulated in FATC vs. EATC. While those miRNAs are known to regulate pathways involved in cancer invasion and metastasis, they were shown to be down-regulated in FATC patients vs. EATC. The significantly top ten down-regulated/upregulated miRNA-signature suggests that it can be a diagnostic, prognostic, and predictive biomarker in TC health disparities.
Citation Format: Ryan Davis, Yan C Wongworawat, Mia C Perez, Krystal Santiago, Saurav Roy, Charles Wang, Alfred A Simental, Salma Khan. Upregulated and downregulated microRNA-signatures in thyroid cancer health disparities [abstract]. In: Proceedings of the Twelfth AACR Conference on the Science of Cancer Health Disparities in Racial/Ethnic Minorities and the Medically Underserved; 2019 Sep 20-23; San Francisco, CA. Philadelphia (PA): AACR; Cancer Epidemiol Biomarkers Prev 2020;29(6 Suppl_2):Abstract nr B093.
Abstract C010: African Americans’ adherence to survivorship
Authors: Albany Roberta A. 
Published: September 2020

Abstract
Looking at data that support issues regarding African Americans not adhering to their taking prescribed medications and following up on their treatment care plans. There’s a need to understand the stigma and social economic/class with regards to poor follow-up on survivorship care plans and we can bridge the gap in the African American communities and health care providers.
Citation Format: Roberta A. Albany. African Americans’ adherence to survivorship [abstract]. In: Proceedings of the Twelfth AACR Conference on the Science of Cancer Health Disparities in Racial/Ethnic Minorities and the Medically Underserved; 2019 Sep 20-23; San Francisco, CA. Philadelphia (PA): AACR; Cancer Epidemiol Biomarkers Prev 2020;29(6 Suppl_2):Abstract nr C010.
Abstract C014: Questions I'd Wished I'd Asked: Cervical Cancer Survivors' Perceptions about Provider Education on Impact of Treatment on Sexual Health
Authors: Hock Gail Petersen 
Published: September 2020

Abstract
This poster shares perspectives of cervical cancer (CC) survivors related to the information they received about how their diagnosis and treatment may impact their sexual health. Mick, Hughes, & Cohen’s (2004) BETTER model was modified into an electronic survey format. The study (n = 89) had 15 variables and two open ended questions. Independent variables were: Age (current and at diagnosis); CC stage; Race; Ethnicity; Insurance status at diagnosis; Educational completion; and Urbanicity. Participants also reported independent variables related to provider gender and type. Dependent variables were five modified BETTER model questions and a question regarding the offer of fertility options following treatment. Open ended questions on the social/cultural impact of diagnosis and the opportunity to share any questions they wished they had asked were included adding depth to the closed ended questions. Outcomes revealed three significant findings. 1) Of respondents reporting that their provider did not explain that they could discuss any concerns during their treatment more than half, of respondents, fell at the lower end of the educational level. 2) There was an association between provider gender and offers of information about fertility preservation. 3) Female providers were 31% more likely to offer fertility information. Although not statistically significant, nearly 50% of the CC survivors reported that issues in the sexual domain were not introduced during discussion of treatment options. This study supports use of the BETTER model as a teaching tool in oncology practices facilitating quality patient centered care by reducing gaps in addressing sexual domain issues related to treatment options. Ultimately, participant perspectives’ will be used to modify public domain educational materials used in oncology practices and cancer support organizations to reflect a more patient centered approach to health in the sexual domain.
Citation Format: Gail Petersen Hock. Questions I'd Wished I'd Asked: Cervical Cancer Survivors' Perceptions about Provider Education on Impact of Treatment on Sexual Health [abstract]. In: Proceedings of the Twelfth AACR Conference on the Science of Cancer Health Disparities in Racial/Ethnic Minorities and the Medically Underserved; 2019 Sep 20-23; San Francisco, CA. Philadelphia (PA): AACR; Cancer Epidemiol Biomarkers Prev 2020;29(6 Suppl_2):Abstract nr C014.
Abstract C036: Disparities in dietary behavior in East Harlem, New York City
Authors: Zambrano Cristina , Lu Wenyue , Johnson Cicely , Beeber Maayan , Panitz April , Ibrahim Safa , Fraser Marilyn , Bhimla Aisha , Tan Yin , Ma Grace , Navder Khursheed , Yeh Ming-Chin , Ogunwobi Olorunseun 
Published: September 2020

Abstract
Cancer is the second most common cause of deaths in the United States, disproportionately affecting minority groups. A diet low in fruits and vegetables is associated with increased risk of cancer. In this multidisciplinary study, we assessed the dietary behaviors and the urinary concentrations of gallic acid, an antioxidant found in various fruits and vegetables, of minorities in New York City. Seventy-five (75) participants were recruited from a senior center in East Harlem, New York City, a racially diverse and underserved community. A National Institute of Health (NIH) - validated survey questionnaire was used to collect dietary behavior data. Demographic and cancer history information were also collected. All 75 participants completed the survey and forty-one (41) participants provided urine samples for urinary gallic acid content analysis. Associations between demographic factors and the intake of certain foods were observed. Specifically, age was negatively associated with the frequency of French fries/fried potatoes and white potatoes intake (p&lt;0.05), while positively associated with frequency of fruit intake (p&lt;0.05). Additionally, Asian race was associated with a higher frequency of fruit intake (p&lt;0.05), compared to other races. Moreover, two male participants (1 Black/African American and 1 White) reported incidences of cancer. Although both individuals reported a college education or higher, they related an annual household income of less than $50,000. In addition, their intake of fruits and vegetables was lower (2.93±1.35) compared to the overall sample (3.38±5.07), along with a much lower detected urinary gallic acid mean concentration (1.78±0.77) compared to the total urinary gallic acid concentration sample mean (11.11±23.14). This study provides preliminary information about the dietary behavior of older adults in East Harlem, which will serve as a basis for a future larger study to investigate the effect of nutrition/dietary education intervention on cancer prevention among diverse groups in New York City.
Citation Format: Cristina Zambrano, Wenyue Lu, Cicely Johnson, Maayan Beeber, April Panitz, Safa Ibrahim, Marilyn Fraser, Aisha Bhimla, Yin Tan, Grace Ma, Khursheed Navder, Ming-Chin Yeh, Olorunseun Ogunwobi. Disparities in dietary behavior in East Harlem, New York City [abstract]. In: Proceedings of the Twelfth AACR Conference on the Science of Cancer Health Disparities in Racial/Ethnic Minorities and the Medically Underserved; 2019 Sep 20-23; San Francisco, CA. Philadelphia (PA): AACR; Cancer Epidemiol Biomarkers Prev 2020;29(6 Suppl_2):Abstract nr C036.
Abstract C037: Development of a physical activity intervention using the socioecological model framework: Formative evaluation among rural African American women
Authors: Hall Marla B. , Mwachofi Ari K. , Collier Caroline B. , Kerwin Kiana L. 
Published: September 2020

Abstract
Due to physical activity (PA) engagement disparities among African Americans (AA), individuals of this population are at increased prevalence for various chronic diseases. Moreover, research suggests that higher levels of PA are linked to lower risk of several cancers. However, within rural areas, locality challenges related to the built environment have been noted as a deterrent for engagement. The purpose of the study was to gather recommendations, using formative evaluation, to develop a physical activity (PA) program for older African American (AA) women (≥ 50 years of age) residing in eastern North Carolina. Focus group (FG) participants were recruited in partnership with local lay health advisors and other trusted community members. Utilizing the socioecological model as a framework, qualitative data were stratified and analyzed using SPSS and NVivo. Participants included a total of 46 women from a convenience sample which consisted of three FGs. At the intrapersonal level, respondents expressed interest in increasing their PA engagement through methods such as walking and/or dancing, and the incorporation of self-monitoring tools to track progress. Regarding interpersonal level suggestions, participants noted the inclusion of group-based approaches. In addition, community level guidance highlighted the scarcity of PA facilities for those residing outside of the county seat, and the necessity of partnering with pre-existing buildings. Lastly, policy level direction was related to environmental infrastructure (i.e., lack of street lights and sidewalks) and personal safety as attributes for inactivity. To circumvent this issue, respondents suggested that outdoor PA sources have consistent law enforcement presence. Data obtained will be used to guide the development of a culturally-tailored and community-advised multilevel PA intervention for older AA women of rural residence.
Citation Format: Marla B. Hall, Ari K. Mwachofi, Caroline B. Collier, Kiana L. Kerwin. Development of a physical activity intervention using the socioecological model framework: Formative evaluation among rural African American women [abstract]. In: Proceedings of the Twelfth AACR Conference on the Science of Cancer Health Disparities in Racial/Ethnic Minorities and the Medically Underserved; 2019 Sep 20-23; San Francisco, CA. Philadelphia (PA): AACR; Cancer Epidemiol Biomarkers Prev 2020;29(6 Suppl_2):Abstract nr C037.
Abstract D007: Mammographic breast density and acculturation: Longitudinal analysis in Chinese immigrants
Authors: Almeida Rebeca , Byrne Celia , Fang Carolyn Y. , Tseng Marilyn 
Published: September 2020

Abstract
Breast cancer is the most common cancer in women, regardless of race or ethnicity. Unlike other racial and ethnic groups, breast cancer rates in Asian American women have steadily increased over the past several decades. The increase among recent migrants might be partly due to the adoption of the behaviors and values of the mainstream culture. We tested the hypothesis that higher level of acculturation was associated with higher mammographic breast density (MBD), an indicator of breast cancer risk, using data from a 2006-2010 longitudinal study of 426 premenopausal Chinese immigrant women in Philadelphia. MBD, including dense area, non-dense area, and percent density, was calculated using a computer assisted method. An abridged version of the General Ethnicity Questionnaire – American version provided a measure of acculturation. We used generalized estimating equations (GEE) to account for repeated observations and adjusted for age, type of mammographic image (film or digital), body mass index, months of breastfeeding, number of live births, age at first birth, and menopausal stage (pre, early peri, late peri, and post). At baseline, the mean age of participants was 43.9 years (SD=4.5), mean length of U.S. residence was 7.5 years (SD=4.8), and mean age at migration was 36.4 years (SD=6.4). GEE models using 1,110 observations from the 426 participants showed no significant associations between acculturation and MBD (percent density β=0.32, p=0.54; dense area β=0.28, p=0.57; and non-dense area β=-0.42, p=0.60). Our findings are consistent with an emerging body of research suggesting that acculturation measures are insufficient to distinguish levels of breast cancer risk in immigrant women, given changes in immigration patterns and economic development in sending countries. Future work should consider other factors, including behavioral, environmental and epigenetic factors or changes in reproductive patterns, that may have a greater effect on MBD than acculturation-related behaviors in adulthood.
Citation Format: Rebeca Almeida, Celia Byrne, Carolyn Y. Fang, Marilyn Tseng. Mammographic breast density and acculturation: Longitudinal analysis in Chinese immigrants [abstract]. In: Proceedings of the Twelfth AACR Conference on the Science of Cancer Health Disparities in Racial/Ethnic Minorities and the Medically Underserved; 2019 Sep 20-23; San Francisco, CA. Philadelphia (PA): AACR; Cancer Epidemiol Biomarkers Prev 2020;29(6 Suppl_2):Abstract nr D007.
Abstract D036: Assessing underrepresented and health disparities investigators’ interests and needed research support in emerging areas of science
Authors: Fleisher Linda , Norbeck Carrie 
Published: September 2020

Abstract
Purpose: The Geographic Management of Cancer Health Disparities Program (GMaP), initiated 2009, is a national program funded by the National Cancer Institute’s Center to Reduce Cancer Health Disparities (CRCHD). GMaP Region 4 is a thirteen state region with over 800 members of underrepresented and health disparities investigators utilizing a multipronged engagement approach to support diverse investigators in their career trajectory including basic, clinical, population and translational research. Understanding how to support these investigators to be successful in new emerging areas of science is critical. Methods: Previous surveys focused on barriers to funding for diversity training awardees and feedback on the value of specific programs, such as travel scholarships supporting early stage investigators (ESI). This current survey focuses additionally on emerging areas of science, including: precision medicine, mHealth, implementation science and commercialization to better understand our members current research interest in these areas and the kinds of support investigators need as they compete in these arenas. This survey is being distributed and data collection and analysis will be completed this summer. Summary of Results: Our previous surveys indicated that ESI’s ranked the following services as “Most Beneficial.” Travel Scholarships – (95.5%); Pilot Funds (for feasibility data collection to support ongoing research) – (, 77.3%); E-blast with job/funding/training resources – (54.5%); Grant Review - (50.0%); and Introductions to CRCHD Program Officers in Training Navigation - (47.6%). We will compare findings from the current survey to determine the ongoing value of these programs as well as the scope of current research in these new emerging areas and the types of support and services required to build investigators research portfolios in these areas. Recommendations: Ongoing programmatic strategies utilized by GMaP Region 4 have been highly valued. The findings of this current survey will provide a deeper understanding of underrepresented and health disparities researchers’ interest in emerging areas of precision medicine, mHealth, implementation science and commercialization as well as recommendations on how to support their research in these areas particularly related to health disparities.
Citation Format: Linda Fleisher, Carrie Norbeck. Assessing underrepresented and health disparities investigators’ interests and needed research support in emerging areas of science [abstract]. In: Proceedings of the Twelfth AACR Conference on the Science of Cancer Health Disparities in Racial/Ethnic Minorities and the Medically Underserved; 2019 Sep 20-23; San Francisco, CA. Philadelphia (PA): AACR; Cancer Epidemiol Biomarkers Prev 2020;29(6 Suppl_2):Abstract nr D036.