bluuebunny/crossref_metadata_2025_split huggingface dataset:
Intra-tumoral genomic heterogeneity in rectal cancer: mutational status is dependent on preoperative biopsy depth and location.
Authors: Vuijk Floris A. , de Water Carlijn van , Vliet Shannon van Lent - van , Valk Maxime J.M. van der , de Velde Cornelis J.H. van , Vahrmeijer Alexander L. , Nagtegaal Iris D. , Hilling Denise E. 
Published: April 2020

Abstract
Background

Neoadjuvant therapy is indicated for all patients with locally advanced rectal cancer, even though a significant number of patients show minimal or no response. Adequate response prediction before the start of neoadjuvant treatment might reduce unnecessary waiting periods and therapy related toxicity in non-responders. Genomic mutational status might provide a means to predict response to neoadjuvant therapy. However, it is unclear whether predictions based on genomic mutational status in single preoperative biopsies are reliable due to intra-tumoral heterogeneity.

In this study we aim to investigate the reliability of genomic mutations found in single pre-operative biopsies by comparing these genomic mutations to 4 other locations within the same tumor using next generation sequencing.

Methods

Rectal cancer patients undergoing primary resection, without neoadjuvant therapy, were included. Of all patients, one biopsy, two deep and two superficial samples were obtained and sequenced using a targeted next generation sequencing gene panel. Concordance between these 5 samples was assessed.

Results

In this feasibility study we included 11 patients. In 7 out of 11 (64%) patients, all 5 samples showed concordant mutations. In 4 out of 11 patients (36%) disconcordant mutations were observed.

Conclusions

Evaluation of genomic mutational status on a single pre-operative biopsy is inadequate in a substantial amount of the patients and is therefore insufficient to predict response to neoadjuvant therapy. Predictive algorithms including multiple parameters might be required to further stratify patients to optimal treatment regimens.
A Structural Equation Modeling of the Wealth and Welfare Components in Iranian households: Rural and Urban Dichotomies
Authors: Shafiei Sediqe , Shakerian Sareh , Yazdani Shahram , Jadidfard Mohammad-Pooyan , Zafarmand A. Hamid 
Published: April 2020

Abstract
Background

Increasing social welfare and reducing poverty are to ensure the well-being of all classes of a society. Cities and villages are distinguished by cultural and economic disparities. The purpose of this study was to develop and present a comprehensive model on welfare and wealth components and their relationship with each other, as well as determining the contributing factors and variables affecting them by presenting a comprehensive model.

Methods

The components of wealth and welfare were determined based on the national data comprising 19261 rural and 18701 urban household participants. Initially, the conceptual model was drawn. Later, observed and latent variables of the model were analyzed and tested using AMOS software, in two exploratory and confirmatory factor analyses. Finally, the Structural Equation Modeling was used to analyze the data and investigate the causal relationship of latent variables.

Results

The findings showed that the factors generating wealth in the urban initiate from education and lead to employment and then into income and ultimately provide wealth and welfare. Plus, there is no separation between the concept of welfare and wealth in urban society. However, land ownership is the prerequisite for wealth and welfare issues in a rural community. Moreover, wealth and welfare are two distinct phenomena in rural areas.

Conclusions

The results of this study would provide the clear hints for effective policy making to resolve deprivation and poverty in Iranian rural and urban populations, prioritizing education for urban and land ownership for rural areas.
Coal quality prediction based on multi-feature fusion of flotation foam images
Authors: bai yateng , ma xiaoping 
Published: April 2020

Abstract
Coal flotation monitoring cannot provide real-time feedback on the yield and ash of coal preparation products because it is influenced by the subjective nature of artificial judgment of coal preparation status and the lag of product quality testing of coal preparation. This paper aims to extract the texture, colour and shape features of floating foam images using various image processing methods, such as colour space, wavelet transform, greyscale co-occurrence matrix and edge operator, and to quantify the characterisation of various characteristic parameters on the basis of the indicative effect of floating foam characteristics on the quality of coal preparation products. The correlation between image features and the yield and ash of flotation products is studied, and a regression prediction model of coal preparation yield and ash was established by combining various image feature parameters using machine learning methods. Experimental results show that the proposed method can realise the real-time monitoring of coal mine flotation and effectively predict coal quality.
Predictive Value of the Surgical Apgar Score on Postoperative Complications in Advanced Gastric Cancer Patients Treated with Neoadjuvant Chemotherapy Followed by Radical Gastrectomy: A Single Center Retrospective Study
Authors: Hayashi Masato , Yoshikawa Takaki , Yura Masahiro , Otsuki Sho , Yamagata Yukinori , Morita Shinji , Katai Hitoshi , Nishida Toshirou 
Published: April 2020

Abstract
Background: The surgical Apgar score (SAS) or modified SAS (mSAS) has been reported as a simple and easy risk assessment system to predict postoperative complications (PCs) in primary surgery for gastric cancer (GC). However, there are still few studies which revealed the SAS’s utility in gastric surgery after neoadjuvant chemotherapy (NAC). Methods: One hundred and fifteen patients who received NAC and R0 gastrectomy from 2008 and 2015 were included in this study. The SAS was determined by estimated blood loss (EBL), lowest intraoperative mean arterial pressure (LMAP), and lowest heat rate (LHR). The mSAS was determined by the EBL reassessed using the interquartile values. The predictive values of the SAS/mSAS for PCs were assessed with uni and multivariate analyses. Results: Among 115 patients, 41 (35.7%) developed PCs. According to analyses with receiver operating characteristic (ROC) curve of the SAS and mSAS for predicting PCs, the cutoff value of the mSAS was set at 8. The rates of anastomotic leakage, pancreatic fistula, and arrhythmia in patients with high mSAS (>8) values were higher, compared to those with low (0-3) and moderate (4-7) mSAS values. A multiple logistic regression analysis detected operation time, Body Mass Index (BMI), and Diabetes Mellitus (DM) were independent risk factors for PCs. The mSAS was not a significant predictor. Conclusions: Neither the SAS nor mSAS was a useful predictor of PCs in patients treated with NAC followed by radical gastrectomy. The predictive value of SAS/mSAS is limited in patients undergoing surgery after NAC.
Significance of calorie-restricted ketogenic diet for lung cancer with brain metastases and hepatoma with pulmonary metastases: report of two cases
Authors: TAN Dong , Zhao Jing , Liu Xiao-jing , Yang Wen-ru , Yuan Fang 
Published: April 2020

Abstract
Cancer cells have altered metabolism that is characterized by an enhanced uptake and utilization of glucose. These increased glucose dependence alterations, present potential vulnerabilities that could be targeted for cancer therapy. The calorie-restricted ketogenic diet (KD) may meet the requirement. In this study, we explore the feasibility of the ketogenic diet as adjuvant therapy for cancer treatment. We present two cases of patients diagnosed with aggressive forms of cancer, lung cancer with brain metastatic tomours and primary liver cancer with pulmonary metastases. The patients began KD intervention after exhausting the other treatment options. Both of them responded to KD and demonstrated a beneficial effect. The tomour size from serial imaging and serum tumour markers were significantly reduced after KD in both subjects without any adverse effects. Both patients had tumour remission even after stopping the ketogenic diet, indicating that KD is a safe, effective treatment to aggressive cancers with metastases when used with conventional therapies.
Relationship between body weight and spinopelvic alignment in Chinese adult people: A preliminary study
Authors: Zhang Qing Xi , Gao Fu Qiang , Wang Yun Ting , Li Zi Rong , Koji Ozaki , Sun Wei 
Published: April 2020

Abstract
Background: The purpose of this study is to evaluate the correlation between spinopelvicalignment and the body weight parameters in healthy Chinese adult volunteers. That has not been systematically studied yet.

Methods: 100 Chinese healthy adult volunteers (36 males and 64 females) were included in this study, which grouped according to the gender. The obesity parameters, body mass index (BMI), waist circumference (WC), sagittal abdominal diameter (SAD), transverse abdominal diameter (TAD) and RR (the ratio of SAD to TAD) were measured. The sagittal spinopelvic parameters include pelvic incidence (PI), pelvic tilt (PT), pelvic angulation (PA), sacral slope (SS), sacral inclination (SI), lumbar lordosis (LL) and the disc angle of L5/S1. The coronal spinopelvic parameters include the Cobb angle of the major curve of the spine, lumbar scoliosis (LS) and pelvic obliquity (PO). Pearson's correlation analysis was used to determine correlations between obesity index and spinopelvic alignment parameters.

Results: The mean BMI and WC of the males and females was 28.7 ± 3.7 kg/m2 vs 26.8 ± 2.3 kg/m2 and 88.39 ± 9 cm vs 82.6 ± 2.7 cm respectively. In the female group, a strong correlation was found between BMI and PI, WC and PT, WC and PI. However, there was no strong correlation between the obesity and spinopelvic alignment parameters in the male group. None of the coronal spinopelvic parameters showed a correlation with the obesity parameters in two groups. The RR showed a positive linear correlation with PA and PT in both groups.

Conclusions: Body weight could influence the spinopelvic alignment,especially for females. People with abdominal obesity, the sagittal spinopelvic alignment is likely to change. But there is little effect on the coronal spinopelvic alignment.
Sequencing of five poultry strains elucidates phylogenetic relationships and divergence in virulence genes in Morganella morganii
Authors: Palmieri Nicola , Hess Claudia , Hess Michael , Alispahic Merima 
Published: April 2020

Abstract
Background

M. morganii is a bacterium frequently associated with urinary infections in humans. While many human strains are sequenced, only the genomes of few poultry strains are available. Here, we performed a detailed characterization of five highly resistant Morganella morganii strains isolated in association with Escherichia coli from diseased domestic Austrian poultry flocks, namely geese, turkeys and chicken layers. Additionally, we sequenced the genomes of these strains by NGS and analyzed phylogenetic clustering, resistance and virulence genes in the context of host-specificity.

Results

Two strains were identified to be Extended Spectrum Beta Lactamase (ESBL) and one as AmpC beta-lactamases (AMP-C) phenotype, while two were ESBL negative. By integrating the genome sequences of these five poultry strains with all the available M. morganii genomes, we constructed a phylogenetic tree that clearly separates the Morganella genus into two clusters (M1 and M2), which approximately reflect the proposed subspecies classification ( morganii and sibonii ). Additionally, we found no association between phylogenetic structure and host, suggesting interspecies transmission. All five poultry strains contained genes for resistance to aminocoumarins, beta-lactams, colistin, elfamycins, fluoroquinolones, phenicol, rifampin and tetracycline. A comparative genomics analysis of virulence genes showed acquisition of novel virulence genes involved in secretion system and adherence in cluster M2. We showed that some of these genes were acquired by horizontal gene transfer from closely related Morganellaceae species and propose that novel virulence genes could be responsible for expansion of tissue tropism in M. morganii . Finally, we detected variability in copy number and high sequence divergence in toxin genes and provided evidence for positive selection in insecticidal toxins genes, likely reflecting host-related adaptations.

Conclusions

In summary, this study describes i) the first isolation and characterization of M. morganii from goose and turkey, ii) a large-scale genetic analysis of M. morganii and an attempt to generate a global picture of the M. morganii intraspecific phylogenetic structure.
Distinct fiber-specific white matter reductions pattern in early- and late-onset Alzheimer’s disease
Authors: Luo Xiao , Wang Shuyue , Jiaerken Yeerfan , Li Kaicheng , Zeng Qingze , Hong Hui , Guan Xiaojun , Guo Tao , Wang Chao , Zhang Ruiting , Zhou Jiong , Wu Dan , Huang Peiyu , Zhang Minming 
Published: April 2020

Abstract
Background Early-onset Alzheimer’s disease (EOAD) has more domain cognitive deficits and worse prognosis than late-onset Alzheimer’s disease (LOAD). However, changes to white matter fiber pathways subserving the structural networks are still unclear, mainly due to the difficulty of modeling crossing-fiber populations. Thus, we used the recently established method, fixel-based analysis (FBA), to investigate fiber-specific white matter (WM) reduction patterns in EOAD and LOAD. Methods We identified 31 EOAD, 45 LOAD, 64 younger and 46 healthy elder controls (YHC/OHC). To repeat results, we further performed FBA in the ADNI database, including 17 EOAD, 30 LOAD, 31 YHC, and 34 OHC. Each subject underwent diffusion MRI scanning and neuropsychological assessments. Subjects from the ADNI database additionally underwent florbetapir and flortaucipir-PET examination. We measured the fiber density (FD) and fiber bundle cross-section (FC), then compared patients versus their counterparts (FWE corrected, P < 0.05). According to fixel-wise results, we further performed the tract of interest analyses. Results In the ZJU database, EOAD had decreased FD in the splenium of CC (SCC), limbic tracts, cingulum bundles, and posterior thalamic radiation (PTR), and decreased FC in the SCC, dorsal cingulum and PTR. By contrast, LOAD had comparable FD and FC decrease, centered to the SCC, cingulum bundles, and posterior association tracts. Both EOAD and LOAD had a similar decrease in FDC, mainly involving the cingulum bundle. Importantly, we found similar trends in the ADNI database. Correlation results showed that both the FD and FC were related to multiple cognitive performance and disease severity. Further, FD of fornix column and body and FC of ventral cingulum correlated with composite amyloid (r = -0.34) and tau level (r = -0.53) reflected by PET imaging, respectively. Conclusions Distinct WM damage in EOAD and LOAD might account for different cognitive pictures. Further, FD and FC might reflect different pathophysiological processes.
Substitution mapping of the major quantitative trait loci controlling stigma exsertion rate from Oryza glumaepatula
Authors: Tan Quanya , Zou Tuo , Zheng Mingmin , Ni Yuerong , Luan Xin , Li Xiaohui , Yang Weifeng , Yang Zifeng , Zhu Haitao , Zeng Ruizhen , Liu Guifu , Wang Shaokui , Fu Xuelin , Zhang Guiquan 
Published: April 2020

Abstract
Background: Stigma exsertion rate (SER) is a key determinant for outcrossing ability of male sterility lines (MSLs) in hybrid rice seed production. Outcrossing ability in cultivated rice varieties has diminished during the process of domestication, while wild Oryza species keep strong outcrossing ability. Here, we detected the quantitative trait loci (QTLs) controlling SER using a set of single-segment substitution lines (SSSLs) derived from O. glumaepatula, a wild Oryza species.Results: Seven QTLs for SER, qSER-1a, qSER-1b, qSER-3a, qSER-3b, qSER-5, qSER-9 and qSER-10, were located on 5 chromosomes. qSER-1a and qSER-1b were located on chromosome 1. qSER-3a and qSER-3b were mapped on chromosome 3, and qSER-3b was further located at an interval of 931.0kb by secondary substitution mapping. qSER-5, qSER-9 and qSER-10 were identified on chromosomes 5, 9 and 10, respectively, and qSER-9 was delimited to a region of 608.2kb by secondary substitution mapping. The additive effects of the 7 QTLs ranged from 10.6% to 14.8%, and the additive contribution variances explained by each of the QTLs were from 36.3% to 50.6%, which were higher than those of most loci for SER reported previously.Conclusions: qSER-1a and qSER-1b were novel loci for SER on chromosome 1. All of the 7 QTLs had major effects on SER. The major QTLs of SER will help to develop MSLs with strong outcrossing ability.
Standardized radical hysterectomy promises the clinical outcomes of laparoscopic surgery for early-stage cervical cancer
Authors: Yang Weihong , Chen Rong , Li Caixia , Li Li , Luo Ning , Cheng Zhongping 
Published: April 2020

Abstract
Background: The recent publication of LACC Clinical Trial (NCT00614211) stated that minimally invasive radical hysterectomy was associated with lower rates of disease-free survival and overall survival than open abdominal radical hysterectomy among women with early-stage cervical cancer (ECC). The purpose of this present study is to evaluate clinical outcomes of laparoscopic standardized radical hysterectomy for ECC. Methods: This is a retrospective controlled study. 328 women with ECC (IA1, IA2, IB1 or IIA1) underwent primary surgery treatments by laparoscopy or laparotomy in Tenth’s People Hospitals. The women diagnosed as stage IB1 or IIA1 performed radical hysterectomy (RH). The total parametrium excision in process of radical hysterectomy was clarified specially in the study. Results: 186 patients underwent open surgery and 142 ones were performed laparoscopic surgery. Laparoscopic surgery was associated with less blood loss (194.43±84.40ml vs. 362.68±253.36ml, P<0.01), shorter hospital staying (11day vs. 14day, P<0.01) and fewer risk of blood transfusion (2.8% vs. 18.8%, P<0.01). There was no statistical difference in postoperative complications between two groups (18/142,12.7% vs. 21/186,11.3% P>0.05). The rate of 5-years overall survival (OS) was 92.8% in laparoscopy group similar to that of 94.4% in open group (p=0.762). Disease-free survival (DFS) rate at 3 years in laparoscopy group significantly decreased when compared to open group (91.8% vs 95.0%, p=0.030). But there was no difference in 3-years DFS among the women with tumor size <2cm (100% vs 97.0%, p=0.818). Discussion: Laparoscopic surgery was associated with preferable surgical quality compared to open surgery in ECC. Laparoscopic radical hysterectomy may be fit to the women with tumor <2cm. Standardized radical hysterectomy helps to promise the clinical outcomes of LRH in ECC.
Effects of light intensity on non-structural carbohydrate contents and C:N:P stoichiometry in Cunninghamia lanceolata and Schima superba
Authors: Liu Qingqing , Huang Zhijun , Wang Zhengning , Chen Yanfang , Wen Zhumei , Liu Bo , Tigabu Mulualem 
Published: April 2020

Abstract
Background: An understanding of the light requirement of tree species has paramount importance in management of mixed species forests. Here, we examined changes in leaf morphological traits, non-structural carbohydrate contents and C:N:P stoichiometry in Cunninghamia lanceolata and Schima superba seedlings that were grown under five light intensity levels (5%, 15%, 40%, 60%, and 100% sunlight) in a shade house. Results: Mean leaf area was significantly larger under 40% light intensity for C. lanceolata while maximum mean leaf area was observed under 15% light intensity for S. superba seedlings, whereas leaf mass per area decreased consistently with decreasing light intensity in S. superba; Non-structural carbohydrate content was higher for S. superba than C. lanceolata when seedlings were exposed to 100%, 15% and 5% light intensity; Leaf C:N ratio decreased while N:P ratio increased with decreasing light intensity; leaf C:P ratio was highest in 40% light intensity for C. lanceolata and in 60% light intensity for S. superba. Conclusion: S. superba is better adapted to low light intensity than C. lanceolata through enlarged leaf area and increased carbohydrate reserves that allow the plant to better maintain C balance. From mixed species planting viewpoint, it would be advisable to plant S. superba later once the canopy of C. lanceolata is well developed but allowing enough sunlight (up to 15%-60%).
SLGT11 Controls Floral Organ Patterning and Floral Meristem Termination in Tomato
Authors: Yang Liling , Qi Shilian , touqeer Arfa , Li Haiyang , Wu Shuang 
Published: April 2020

Abstract
Background: Flower development affects fruit production directly in tomato. Despite the framework mediated by ABC genes have been established in Arabidopsis, the spatiotemporal precision of floral development in tomato has not been well examined.Results: Here, we analyzed a novel tomato mutant in which the normal development of stamens and carpels failed, resulting in ectopic formation of floral and shoot apical meristem in the fourth whorl position, which later developed into stem- and leaf-like structures. Using bulked segregant analysis (BSA), we assigned the causal mutation to the gene SLGT11 that encodes a transcription factor belonging to Trihelix gene family. Further RNAi silencing of SLGT11 verified the defective phenotypes of slf mutant. The failed termination of floral meristem and the occurrence of floral reversion in slf mutant indicate that SLGT11 functions as a non-canonical C type gene. Furthermore, we found that the defects in slf were substantially enhanced at higher temperature, with petals transformed into sepals, all stamens disappeared, and increased frequency of ectopic floral meristem.Conclusions: Together with the spatiotemporal expression pattern, we suggest that SLGT11 functions in floral organ patterning and termination of floral meristem identity in tomato.
Patients’ and tutors’ assessment of General Practice/Family Medicine consultations conducted by medical students in Coimbra, Portugal
Authors: Santiago Luiz Miguel , Silva Inês Rosendo , Simões José Augusto 
Published: April 2020

Abstract
Background: Undergraduate teaching of General Practice/Family Medicine (GP/FM) must ensure students acquire the necessary competencies and skills to perform an adequate GP/FM consultation with adequate annotations (the SOAP model) and classifications. So aimed to study and to correlate students’ evaluation by tutors and patients in specific consultations in the formal practical evaluation of GP/FM Curricular Unit of the Integrated Masters on Medicine at the Faculty of Medicine of the University of Coimbra (IMM-FMUC) in the academic years of 2017-2018 and 2018-2019.Methods: Observational study of the 2017-2018 and 2018-2019 academic years of the assessment grids for tutor’s evaluation of SOAP performance and fluency in consultation and for patient’s evaluation of the student ‘performance, in the convenience sample of those who chose to be so evaluated.Results: We studied a population of 435 (67,7%) out of a universe of 646 students, 125 (28,7%) males, ns by sex and academic year who performed this evaluation. In a mark up to 20 from tutors, difference was found for Plan (P) mark, higher in 2018-2019 (18,38±2,18vs18,54±2,11, p=0,005) of the SOAP methodology evaluation. Patients’ evaluation was not different 19,34±1,70vs19,35±1,40, p=0,091. A positive significant correlation was found between tutors and patients marks (ρ=0,278; p<0,001), as well as between tutor mark and final mark (ρ=0,958; p<0,001) and patient and final marks (ρ=0,465; p<0,001). Final marks were not different in both years, 18,61±1,38vs18,78±1,15, p=0,158.Conclusions: This innovative model of evaluation of student’s performance in medical appointment, showed a significant positive moderate correlation between patients’ and tutors’ marks in the setting of GP/FM at the IMM-FMUC, and was not different between years. Yearly evaluation must be continued.