bluuebunny/crossref_metadata_2025_split huggingface dataset:
Activating Effect of 3‐Benzylidene Oxindoles on AMPK: From Computer Simulation to High‐Content Screening
Authors: Novikova Daria S. , Grigoreva Tatyana A. , Ivanov Gleb S. , Melino Gerry , Barlev Nickolai A. , Tribulovich Vyacheslav G. 
Published: August 2020

AbstractAMP‐activated protein kinase (AMPK) is currently the subject of intensive study and active discussions. AMPK performs its functions both at the cellular level, providing the switch between energy‐consuming and energy‐producing processes, and at the whole body level, particularly, regulating certain aspects of higher nervous activity and behavior. Control of such a ‘main switch’ compensates dysfunctions and associated diseases. In the present paper, we studied the binding of 3‐benzylidene oxindoles to the kinase domain of the AMPK α‐subunit, which is thought to prevent its interaction with the autoinhibitory domain and thus result in the AMPK activation. For this purpose, we developed the cellular test system based on the AMPKAR plasmid, which implements the FRET effect, synthesized a number of 3‐benzylidene oxindole compounds and simulated their binding to various sites of the kinase domain. The most probable binding site for the studied compounds was established by the correlation of calculated and experimental data. The obtained results allow to analyze various classes of AMPK activators using virtual and high‐content screening.
Theory and algorithm of fire alarm coverage control in network
Authors: Li Mengjie , Liu Chao 
Published: August 2020

SummaryFire is a serious threat to human life and property, and effective prediction of fire can reduce the harm of fire to human. In the research of fire alarm automation, the fire alarm system based on wireless sensor network is a common method. When using wireless sensor to monitor the fire alarm information, it is necessary to ensure the safety coverage of the network in the monitoring area. Therefore, it is very necessary to study the network coverage in the fire alarm monitoring, which can ensure the effective work of the monitoring network, and good coverage control can reduce the energy consumption of the network and extend the service life of the network. Based on this, this paper designs a network coverage control algorithm based on multiobjective genetic algorithm optimization considering the coverage and energy consumption of wireless network. In this algorithm, the probability perception model is used to design the network coverage control model for the monitoring area with different coverage requirements, taking the maximum area coverage and the minimum network energy consumption rate as the optimization goal. Then the genetic algorithm is used to solve the model. In addition, because the multiobjective genetic algorithm is easy to fall into the local optimal solution when solving the optimization problem, this paper introduces the cross and variation coefficients of self‐adaptive adjustment to improve the genetic algorithm. Through the simulation analysis, it is shown that when solving the multiobjective optimization problem, the introduction of self‐adaptive adjustment of cross and variation coefficients can well overcome the problem that genetic algorithm falls into the local optimal solution, whereas the network coverage control algorithm designed in this paper based on the multiobjective genetic algorithm optimization can achieve greater coverage and lower energy consumption. Compared with the network coverage control algorithm before optimization, the optimized algorithm improves greatly in coverage, network residual energy, and number of nodes, which can well extend the service life of wireless sensor network, and is conducive to the good application of wireless sensor in fire prediction.
Acinic cell carcinoma of the salivary gland associated with lymphoid‐rich stroma. A diagnostic dilemma on cytology: Study of two cases
Authors: Mir Fatima , Rohra Prih , Aakash Nfn , Furlan Karina , Cheng Lin , Ghai Ritu , Reddy Vijaya , Gattuso Paolo 
Published: August 2020

AbstractA lymphoid‐rich stroma is a common finding in salivary gland tumors. Several reports documented this association with acinic cell carcinoma (ACC). However, cytologic studies reporting this phenomenon are rare and mainly confined to sporadic single case reports. We present the cytologic features of two cases of ACCs of the parotid gland displaying a lymphoid‐rich background and discuss the cytologic differential diagnoses of this uncommon ACC variant.
The Caenorhabditis elegans proteasome subunit RPN‐12 is required for hermaphrodite germline sex determination and oocyte quality
Authors: Fernando Lourds M. , Elliot Jeandele , Allen Anna K. 
Published: August 2020

AbstractBackgroundThe proteasome is a multi‐subunit complex and a major proteolytic machinery in cells. Most subunits are essential for proteasome function, and depletion of individual subunits normally results in lethality. RPN‐12/Rpn12/PSMD8 is a lid subunit of the 19S regulatory particle (RP) of the 26S proteasome. Studies in Caenorhabditis elegans demonstrated that RNAi depletion of RPN‐12 does not result in lethality. RPN‐12 has not been well studied in higher eukaryotes. In this study, we investigate the biological significance of RPN‐12 in C. elegans.ResultsWe found that the null mutant rpn‐12(av93) did not cause major impairment of the proteolytic activity of the proteasome. Most rpn‐12(av93) hermaphrodites lack sperm leading to feminization of the germ line that can be partially rescued by mating to males. The lack of sperm phenotype can be suppressed by downregulation of TRA‐1, a player in the hermaphrodite germline sex determination pathway. Also, rpn‐12(av93) animals show significant nuclear accumulation of the meiotic kinase WEE‐1.3, a protein predominantly localized to the perinuclear region. Interestingly, chemical inhibition of the proteasome did not cause nuclear accumulation of WEE‐1.3.ConclusionsRPN‐12 plays a previously unknown role in oogenesis and the germline sex determination pathway in C. elegans hermaphrodites.
Variability in infragravity wave processes during estuary artificial entrance openings
Authors: McSweeney Sarah L. , Stout Justin C. , Kennedy David M. 
Published: August 2020

ABSTRACTIntermittently open/closed estuaries (IOCE) are wave‐dominated estuaries with entrances that temporarily close to the ocean. Wave–current interactions play a major role in estuary entrance morphodynamics and influence the degree of energy transfer from the ocean into the lagoon. This study utilizes artificial entrance openings of multiple ICOE in Victoria, Australia, to capture continuous hydrodynamic and geomorphic data throughout the opening cycle. We illustrate that water level oscillations in the infragravity (IG) band) are present in the basin during open entrance conditions. IG waves were observed to propagate up to 1.8 km upstream of the mouth while the entrance was open. Our work identifies that changes in cross‐sectional area, bed depth at the berm position, and offshore wave height control the magnitude of IG waves within the estuary basin. IG wave magnitude is also tidally modulated and increases with high tides when the nearshore water level is higher. Late during the drainage phase, waves were observed to track the margins of the channel, away from the thalweg, and reach the basin. IG wave energy was highest immediately after the basin had ceased draining and while channel dimensions at the mouth were within 10% of their maximum value. As the entrance aggrades, IG wave magnitude decreases in the absence of energetic offshore wave conditions. We relate the changes in IG wave magnitude and frequency to a six‐stage conceptual model of the opening–closure sequence. Within the basin, IG wave energy, height and frequency were also consistently highest closer to the mouth and decreased with distance upstream. Our findings indicate that water level oscillations in the IG band are a persistent feature in IOCE and may be the norm rather than the exception in these systems. As IG waves were captured serendipitously as part of a larger field campaign, future work will focus on instrumenting IOCE to gain high‐resolution data to quantify IG wave processes during entrance openings. © 2020 John Wiley & Sons, Ltd.
Development of a Reduced-Volume Acute Lethality Toxicity Test for Hyalella azteca
Authors: Rodrigues Maegan R. , Frank Richard A. , Schissler Daniel M. , Deeth Lorna E. , Brown Lisa R. , Hedges Amanda M. , Dixon D. George , Hewitt L. Mark , Bartlett Adrienne J. 
Published: August 2020

Abstract
Effects-directed analysis (EDA) is used to identify the principal toxic components within a complex mixture using iterative steps of chemical fractionation guided by bioassay results. Bioassay selection can be limited in EDA because of the volume requirements for many standardized test methods, and therefore, a reduced-volume acute toxicity test that also provides whole-organism responses is beneficial. To address this need, a static, 7-d, water-only, reduced-volume method (50 mL, 10 organisms) was developed for Hyalella azteca that substantially decreases the volume requirements of standard-volume acute test exposures (200–500 mL of test solution, 15–20 organisms) while maintaining water quality and meeting control survival criteria. Standard- and reduced-volume methods were compared by conducting concurrent toxicity tests with 2 inorganic toxicants (KCl and CdCl2) and 2 organic mixtures of naphthenic acid fraction components (NAFCs) to evaluate test performance. There was no difference between methods when comparing the median lethal concentrations (LC50s) for KCl and both NAFC mixtures (p &gt; 0.05). The LC50s for CdCl2 were statistically different (p = 0.0002); however, this was not considered biologically meaningful because the difference between LC50s was &lt;2-fold. In conclusion, the reduced-volume H. azteca test method generated results comparable to standard-volume test methods and is suitable for use in situations where limited testing material is available, such as when conducting EDA. Environ Toxicol Chem 2020;39:2221–2227. © 2020 Her Majesty the Queen in Right of Canada. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC. Reproduced with the permission of the Minister of Environment and Climate Change Canada.
Erweiterte Anwendung der Barodesie für Finite‐Elemente‐Berechnungen
Authors: Bode Manuel , Medicus Gertraud , Fellin Wolfgang 
Published: August 2020

AbstractIn diesem Aufsatz werden erweiterte Anwendungsmöglichkeiten der Barodesie bei Finite‐Elemente‐Berechnungen aufgezeigt. Dabei wird zum einen die Anwendung des Konzepts der intergranularen Dehnung zur Berücksichtigung der Steifigkeitserhöhung im Bereich kleiner Verzerrungen mit der dazugehörigen FE‐Implementierung vorgestellt. Durch die Verwendung der sog. asymptotischen State Boundary Surface (ASBS) der Barodesie können die Effekte des Überschreitens zulässiger Zustände mit der intergranularen Dehnung reduziert werden, womit eine realistischere Verformungsprognose möglich ist. Zum anderen wird ein verbesserter Ansatz zur Standsicherheitsberechnung mittels Parameterreduktion für die Barodesie präsentiert. Anhand einfacher FE‐Anwendungen werden die vorgestellten Erweiterungen verifiziert und deren Einfluss auf das Berechnungsergebnis untersucht.
Moderately accelerated intensity‐modulated radiation therapy using simultaneous integrated boost: Practical reasons or evidence‐based choice? A critical appraisal of literature
Authors: De Felice Francesca , Bonomo Pierluigi , Sanguineti Giuseppe , Orlandi Ester 
Published: August 2020

AbstractConcurrent chemo‐radiotherapy is the non‐surgical mainstay of treatment for locally advanced head and neck squamous cell carcinoma (HNSCC). The following aspects have emerged as fundamental components of the combined approach: first, intensity modulated radiotherapy (IMRT) is the minimum standard technical requirement, with level 1 evidence in support of its reduction of late treatment‐induced morbidity in comparison with 3D conformal radiotherapy. Second, cisplatin‐based chemotherapy is the preferred systemic agent to be associated with radiation, with 100 mg/m2 every 3 weeks deemed as the reference schedule. Because of significant progress in irradiation techniques achieved in last 15 years, the optimal fractionation schedule in modern radiation era remains controversial, especially for locally advanced disease. The purpose of this work was to perform a critical review on the value of moderately accelerated IMRT using simultaneous‐integrated boost (SIB) in HNSCC, aiming to provide insights on current clinical practice and directions for future research.
Financial contagion across G10 stock markets: A study during major crises
Authors: BenSaïda Ahmed , Litimi Houda 
Published: August 2020

AbstractThe multiple financial crises that occurred during the last two decades have stimulated scholars to investigate the cross‐market linkages and how shocks are transmitted across borders. Researchers usually examine financial contagion and its effects during a single period of study based on static models. This paper employs a regular vine copula specification to model the dependence dynamics across the G10 stock markets, by analysing the impacts during tranquillity periods and during each crisis episode studied apart. Empirical findings show significant differences in the connectedness, with distinctive shock transmission paths during every sub‐period. The results suggest a strong evidence of contagion during the global financial crisis and the European sovereign debt crisis. However, during the market downturn of 2002, there was no significant contagion due to the lack of market integration then. Besides, in the aftermath of the Brexit vote, Eurozone members took measures to reduce possible shock transmission and eliminate contagion.
Synthesis of new series of thiazol‐(2(3H)‐ylideneamino)benzenesulfonamide derivatives as carbonic anhydrase inhibitors
Authors: Berber Nurcan , Arslan Mustafa , Vural Fırat , Ergun Adem , Gençer Nahit , Arslan Oktay 
Published: August 2020

AbstractHuman carbonic anhydrase I and II isoenzymes (hCA I and II) are important metabolic enzymes. In this study, a new series of thiazol‐(2(3H)‐ylideneamino)benzenesulfonamide derivatives were synthesized and also some inhibition parameters including IC50 (hydratese) and inhibition constant values (Ki, esterase) were determined. All studied compounds exhibited potent inhibition against these enzymes. They inhibited carbonic anhydrases (CAs) with the IC50 values of 113 to 395.8 nM (Ki = 77.38‐319.59 nM) for hCA I and 91.9 to 516 nM (Ki = 62.79‐425.89 nM) for hCA II. Among the compounds, 5c was found to be the most active one (Ki: 77.38 nM) for hCA I and 5g was found for hCA II with the value of 62.79 nM.
Nonsuicidal self‐injury, difficulties in emotion regulation, negative urgency, and childhood invalidation: A study with outpatients with eating disorders
Authors: Vieira Ana I. , Moreira Célia S. , Rodrigues Tânia F. , Brandão Isabel , Timóteo Sertório , Nunes Patrícia , Gonçalves Sónia 
Published: August 2020

AbstractObjectivesAmong outpatients with eating disorders (ED), we compared participants without nonsuicidal self‐injury (non‐NSSI group), with NSSI over a year ago (past NSSI group) and with NSSI in the previous year (current NSSI group) regarding different variables, and examined whether difficulties in emotion regulation and negative urgency moderated the relationship between maternal/paternal invalidation and NSSI.MethodThe sample included 171 outpatients (94.2% female; Mage = 28.78, SDage = 11.19).ResultsFifty‐four participants (31.6%) had NSSI in the previous year. This group showed higher eating pathology, difficulties in emotion regulation, negative urgency, and maternal/paternal invalidation than the non‐NSSI group. Analyses revealed an adequate fit to the data for the model that included moderating effects of emotional awareness and negative urgency in the relationship between maternal/paternal invalidation and increased likelihood of NSSI in the previous year.ConclusionsInterventions for NSSI and ED should include emotion regulation, impulse control, and validation strategies.
An analysis of question style and type in official Finnish asylum interview transcripts
Authors: Skrifvars Jenny , Korkman Julia , Sui Veronica , van Veldhuizen Tanja , Antfolk Jan 
Published: August 2020

AbstractInterviews with asylum seekers are an important part of investigating the applicant's need of international protection. Few studies have examined if the questions used in interviews allow detailed and accurate narratives. In the current study, we analysed question style, question type, and question order from interviews of 80 official asylum cases realised by Finnish state authorities in 2017–2018. In accordance with best practise, questions were predominantly asked in an information‐gathering style. However, four‐fifths of the questions were closed questions, and one‐tenth were open questions. The recommended question order was followed to a small degree. Possibilities on how to improve the quantity and the accuracy of the information elicited from the interviews are discussed. Future research should assess which type of questions that are most efficient in eliciting relevant information within the asylum context as well as investigating the accuracy of the interpretation.
Transmission dynamics of SARS‐CoV‐2 within families with children in Greece: A study of 23 clusters
Authors: Maltezou Helena C. , Vorou Rengina , Papadima Kalliopi , Kossyvakis Athanasios , Spanakis Nikolaos , Gioula Georgia , Exindari Maria , Metallidis Symeon , Lourida Athanasia N. , Raftopoulos Vasilios , Froukala Elisavet , Martinez‐Gonzalez Beatriz , Mitsianis Athanasios , Roilides Emmanuel , Mentis Andreas , Tsakris Athanasios , Papa Anna 
Published: August 2020

AbstractThere is limited information on severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) infection clustering within families with children. We aimed to study the transmission dynamics of SARS‐CoV‐2 within families with children in Greece. We studied 23 family clusters of coronavirus disease 2019 (COVID‐19). Infection was diagnosed by reverse‐transcriptase polymerase chain reaction in respiratory specimens. The level of viral load was categorized as high, moderate, or low based on the cycle threshold values. There were 109 household members (66 adults and 43 children). The median attack rate per cluster was 60% (range: 33.4%‐100%). An adult member with COVID‐19 was the first case in 21 (91.3%) clusters. Transmission of infection occurred from an adult to a child in 19 clusters and/or from an adult to another adult in 12 clusters. There was no evidence of child‐to‐adult or child‐to‐child transmission. In total 68 household members (62.4%) tested positive. Children were more likely to have an asymptomatic SARS‐CoV‐2 infection compared to adults (40% vs 10.5%; P = .021). In contrast, adults were more likely to develop a severe clinical course compared with children (8.8% vs 0%; P = .021). In addition, infected children were significantly more likely to have a low viral load while adults were more likely to have a moderate viral load (40.7% and 18.6% vs 13.8% and 51.7%, respectively; P = .016). In conclusion, while children become infected by SARS‐CoV‐2, they do not appear to transmit infection to others. Furthermore, children more frequently have an asymptomatic or mild course compared to adults. Further studies are needed to elucidate the role of viral load on these findings.
On the changing relationship between North Pacific climate variability and synoptic activity over the Hawaiian Islands
Authors: Elison Timm Oliver , Li Siyu , Liu Jiping , Beilman David W. 
Published: August 2020

AbstractThe teleconnection between tropical and extratropical climates in the North Pacific and continental regions of eastern Asia and western North America is known to vary on decadal to multidecadal time scales. In this study, the teleconnection pattern is studied with observational and reanalysis data products. The regional focus is set on the Hawaiian Islands in the central subtropical part of the North Pacific. By analysing correlations between regional climate indices and large‐scale climate modes during the years 1980 and 2014, it was found that the correlation between El Niño—Southern Oscillation (ENSO) and the synoptic weather activity over the Hawaiian Islands decreased over time. Composite analysis of the geopotential height anomalies and upper level winds suggest that the systematic shift in the North Pacific Jet (NPJ) position had an impact on the teleconnection between tropical Pacific SST and winter storm activity and precipitation variability in Hawai'i. The change in the correlations and in the NPJ structure coincides with a transition from the positive phase of the Pacific Decadal Oscillation (PDO) towards a neutral and weak negative state. This observation‐based study provides a central subtropical Pacific viewpoint in support of the growing body of research studies that have reported a major shift in the Pacific climate system during the mid‐1990s. The article further discusses the potential role of decadal‐scale changes in the North Pacific Oscillation (NPO) phase in changing the strength of the ENSO teleconnection with synoptic activity over the Hawaiian Islands. The results of this study are relevant to paleoclimate interpretation of individual proxy records as well as for regional downscaling of future rainfall for the Hawaiian Islands.
Millennials are not all the same: Examining millennial craft brewery and winery visitors' social involvement, self‐image, and social return
Authors: Bachman Jarrett R. , Hull John S. , Haecker Sanja 
Published: August 2020

AbstractThe attraction of craft breweries and wineries for visitors to British Columbia (BC), Canada, has grown significantly in recent years, particularly within millennials. However, little research has been conducted. The present research surveyed millennial visitors across craft breweries and wineries in BC. A total of 359 usable surveys were collected. Results found that although craft brewery and winery visitors had similar demographics, significant differences were found in all three social factors measured. In addition, the conceptual relationships between social involvement and self‐image congruency, as well as social return, were significant. Recommendations and implications for researchers, marketers, and industry professionals are discussed.