P3528Circulating microRNA-210 concentrations are increased in patients with acute heart failure and provide prognostic information
Authors: Rutkovskiy A , Lyngbakken M N , Dahl M B , Bye A , Pedersen M H , Wisloff U , Christensen G , Hoiseth A D , Omland T , Rosjo H 
Published: October 2019

Abstract

Background
MicroRNA (miR)-210 is induced by cellular hypoxia and circulating miR-210 concentrations are associated with clinical outcome in patients with myocardial infarction and aortic stenosis. Whether circulating miR-210 concentrations provide diagnostic and prognostic information in unselected patients with dyspnea is not known.

Purpose
To assess the diagnostic and prognostic value of circulating miR-210 concentrations in patients hospitalised due to acute dyspnea.

Methods
We extracted microRNA from plasma samples obtained on admission from 314 patients hospitalised for acute dyspnea and 10 healthy control subjects. miR-210 concentrations were measured by quantitative polymerase chain reaction and we used miR-425 for normalisation. The merit of circulating miR-210 concentrations to diagnose and provide prognostic information in patients with acute heart failure (HF) was compared to the merit of N-terminal pro-B-type natriuretic peptide (NT-proBNP).

Results
In total, 143 patients (46%) were adjudicated as hospitalised due to acute heart failure (HF) and 84 patients (27%) due to acute exacerbation of chronic obstructive lung disease (AECOPD). All patients and control subjects had miR-210 concentrations within the range of detection (Cq 26–32) and analytical variation was low. miR-210 concentrations correlated with age, NT-proBNP and cardiac troponin T concentrations in the total cohort. Circulating miR-210 concentrations were increased in patients with HF (4.7±3.3 fold increase, p<0.0001) and AECOPD (3.4±1.7 fold increase, p<0.0001) compared to control subjects. Circulating miR-210 concentrations were not different between patient groups and receiver operating characteristics area under the curve (AUC) for miR-210 to diagnose acute HF was 0.50 (95% CI 0.43–0.57) compared to AUC 0.85 (0.81–0.89) for NT-proBNP. During a median 817 days of follow-up, 66 patients (46%) with acute HF died and 35 patients (42%) with AECOPD died. Circulating miR-210 concentrations separated acute HF patients with a poor and favourable outcome (Figure 1; p by the log rank test =0.017). Circulating miR-210 concentrations were also associated with mortality during follow-up in Cox regression model: hazard ratio (HR) for lnRQ of miR-210 was 2.11 (95% CI 1.27–2.50), p=0.004. The association between circulating miR-210 concentrations and outcome was attenuated and no longer significant after adjusting for NT-proBNP concentrations. Circulating miR-210 concentrations did not predict outcome in patients with AECOPD: HR 1.38 (0.65–2.93); p=0.4.
Figure 1

Conclusions
Circulating miR-210 concentrations are increased in patients with acute HF, and provide prognostic information during follow-up. Still, circulating miR-210 concentrations did not diagnose acute HF among unselected patients with dyspnea and the association with outcome was attenuated by NT-proBNP.

Acknowledgement/Funding
Nasjonalforeningen for Folkehelsen