bluuebunny/crossref_metadata_2025_split huggingface dataset:
AKT inhibitor SC66 inhibits proliferation and induces apoptosis in human glioblastoma through down-regulating AKT/β-catenin pathway
Authors: Gao Lun , Liu Junhui , Xu Pengfei , Deng Gang , Liu Baohui , Yuan Fanen , Tan Yinqiu , Sun Qing , Xu Yang , Zhang Huikai , Qi Yangzhi , Han Shoumeng , Yang Kun , Geng Rongxin , Jiang Hongxiang , Chen Qianxue 
Published: March 2020

Abstract
Background ： Glioblastoma multiforme (GBM) is the most common and deadliest type of primary malignant tumor in the adult central nervous system. Temozolomide (TMZ) has limited effectiveness on glioblastoma, so it is urgent to develop new drugs to improve the prognosis of patients. SC66, a novel AKT inhibitor, was reported to exert antiproliferative activity in many types of cancer cells. However, it remains unclear whether SC66 has antitumor effects in GBM. Methods : Cell count kits-8(CCK8) assay, EdU-DNA incorporation assay and colony formation assay were used to evaluate cell proliferation of U87 and U251 cells.Wound -healing assay and transwell assay were used to detect migration and invasion. The cell cycle and apoptosis were detected by flow cytometry. Finally, xenograft mouse model was established to demonstrate the antitumor effect of SC66 in vitro. Results :SC66 obviously suppressed U87 and U251 cells proliferation in a dose-dependent manner. Additionally, SC66 treatment was found to significantly inhibit the invasion and migration of GBM cells as detected by Transwell invasion and wound healing assays. Moreover, treatment of SC66 induced GBM cells apoptosis through up-regulating BAX, Cleaved -caspase3 and down-regulating Bcl-2. SC66 also could arrested cell cycle in G0/G1 phase by decreasing cyclin D1.Furthermore, the results of western blot showed that SC66 significantly reduced level of phosphorylation of AKT, p-GSK-3β and β-catenin, while no change was observed in level of AKT and GSK3β.Then a GSK3-inhibitor,IM-12 was used and IM-12 could significantly restored proliferation, migration and invasion of glioma cells treated with SC66.Meanwhile, SC66 showed significantly suppressed the tumorigenicity compared to the control group in the xenograft mouse model. Conclusion : AKT inhibitor SC66 exerted powerful antitumor activity via down-regulating AKT/β-catenin pathways in vitro and in vivo. It might be as a potential chemotherapy drug to improve the therapeutic efficacy of GBM treatments.
FABP7 is a potential biomarker to predict response to neoadjuvant chemotherapy for breast cancer
Authors: Xie Qin , XIAO Ying-sheng , JIA Shi-cheng , ZHENG Jie-xuan , DU Zhen-chao , CHEN Yi-chun , CHEN Mu-tong , LIANG Yuan-ke , LIN Hao-yu , zeng de 
Published: March 2020

Abstract
Background Early prediction of response to neoadjuvant chemotherapy (NAC) is critical in choosing appropriate chemotherapeutic regimen for patients with locally advanced breast cancer. Herein, we sought to identify potential biomarkers that could predict the response to neoadjuvant chemotherapy for breast cancer patients. Methods Three genomic profiles acquired by polymerase chain reaction (PCR) from subjects with or without residual tumors after NAC that downloaded from the GEO database were used to screen the differentially expressed genes (DEGs). An array of public databases, including ONCOMINE, cBioportal, Breast Cancer Gene Expression Miner v4.0, and the Kaplan Meir-plotter, etc., were used to evaluate the potential functions, related signaling pathway, as well as prognostic values of FABP7 in breast cancer. Anti-cancer drug sensitivity assay, real-time PCR, ﬂow cytometry and western-blotting assays were used to confirm the function of FABP7 in BC cells and examine the relevant mechanism. Results Two differentially expressed genes, including FABP7 and ESR1, were identified to be potential indicators of response to anthracycline and taxanes chemotherapy for breast cancer. FABP7 is associated with better chemotherapeutic response, while ESR1 was associated with poorer chemotherapeutic effectiveness. Generally, the expression of FABP7 was significantly lower in breast cancer than normal tissue samples. FABP7 mainly high expressed in ER-negative breast tumor and might regulate cell cycle to enhance chemosensitivity. Moreover, elevated FABP7 expression increased the percentage of cells at both S and G2/M phase in MDA-MB-231-ADR cells, and decreased the percentage of cells at GO/G1 phase, as compared to control group. Western-blotting results showed that elevated FABP7 expression could increase Skp2 expression, while decrease CDH1 and p27kip1 expression in MDA-MB-231-ADR cells. In addition, FABP7 was correlated to longer recurrence-free survival (RFS) in BC patients with ER-negative subtype of BC treated with chemotherapy. Conclusion FABP7 is a potential favorable biomarker and predicts better response to NAC in breast cancer patients. Future study on the predictive value and detail molecular mechanisms of FABP7 in contribution to chemosensitivity in breast cancer is warranted.
MicroRNA-19b-3p suppresses gastric cancer development by negatively regulating Neuropilin-1
Authors: Wei Yingfeng , Guo Sheng , Tang Jianhua , Wen Jianjun , Wang Huifen , Hu Xiaobo , Gu Qiuping 
Published: March 2020

Abstract
Background Gastric cancer (GC) remains one of the most common digestive malignancies worldwide and ranked third causes of cancer-related death. Mounting evidence has revealed that miRNAs exert critical regulatory roles in GC development. Methods Immunohistochemistry (IHC) and western blot assay were performed to determine the protein expression levels of neuropilin-1 (NRP1) and mRNA levels were confirmed by quantitative RT-PCR (qRT-PCR) in GC tissues. Kaplan–Meier analysis was performed to evaluate the prognostic value of NRP1 in GC. Knockdown of NRP1 was conducted to analyse its function in vitro and vivo. Luciferase reporter assay, western blot and qRT-qPCR were employed to identify the miRNAs which directly targeted NRP1. Furthermore, Bioinformatics analysis and experimental verification were used to explore the potential molecular mechanism and signaling pathway. Results In the current study, we revealed that neuropilin-1 (NRP1) was highly expressed in GC tumor tissues and was associated with poor prognosis in GC patients. NRP1 knockdown inhibited GC cell growth, migration and invasion in vitro, while suppressed GC xenograft tumor development in vivo. Bioinformatics analysis predicted that miR-19b-3p down-regulated NRP1 expression by targeting its 3’-UTR. Functional assay demonstrated that miR-19b-3p inhibited GC cell growth, migration and invasion via negatively regulating NRP1. Overexpression NRP1 partially reversed the regulatory effect of miR-19b-3p. Moreover, we showed that miR-19b-3p/NRP1 axis regulated the epithelial-to-mesenchymal transition and focal adhesion in GC, which might contribute the GC development and progression. Conclusions Taken together, our findings suggest a regulatory network of miR-19b-3p/NRP1 in GC development. The miR-19b-3p/NRP1 axis might be further explored as a potential diagnostic and therapeutic target in GC.
iTRAQ protein profile analysis of leaves and roots of sugar beet (Beta vulgaris) differing in response to salt stress
Authors: Li Junliang , Cui Jie , Cheng Dayou , Dai Cuihong , Liu Tianjiao , Wang Congyu , Luo Chengfei 
Published: March 2020

Abstract
Background

Salinity is one of the most serious threat to agriculture worldwide. Sugar beet is an important sugar-yielding crop and has a certain tolerance to salt. However, the molecular mechanism of salt tolerance in beta vulgaris are poorly understood. Proteomics can provide a new perspective and deeper understanding for the research of beet salt-tolerant.

Results

Here, leaves and roots were used to identify the differentially abundant protein species between salt-stress and control conditions in beta vulgaris. As a result, 70 and 76 DAPs were identified in leaves and roots, respectively. The functions were determined for the classification of the DAPs, mainly involved in cellular processes, environmental information processing, genetic information processing and metabolism. These processes can work cooperatively to reconstruct the favorable equilibrium of physiological and cellular homeostasis under salt stress. Some candidate DAPs are closely related to salt resistance such as choline monooxygenase, betaine aldehyde dehydrogenase, glutathione S-transferase (GST) and F-type H+-transporting ATPase. The expressional pattern of 10 DAPs encoding genes were consistent with the iTRAQ data.

Conclusions

Our results demonstrated that during adaptation of beet to salt stress, leaves and roots have distinct mechanisms of molecular metabolism regulation. This study provided some significative insights into the molecular mechanism underlying the response of higher plant to salt stress, and identified some candidate proteins against salt stress.
Using lipidomics to reveal details of lipid accumulation in developing Siberian apricot (Prunus sibirica L.) seed kernels
Authors: Mai Yiting , Huo Kaisen , Yu Haiyan , Zhou Nan , Shui Lanya , Liu Yang , Wang Nenglong , Zhang Chengxin , Niu Jun , Wang Libing 
Published: March 2020

Abstract
Background The oil content of Siberian apricot seed kernel (SASK) is generally as high as 50%, and biodiesel fuel properties of SASK oil were conformed to EN 14214 and GB/T 20828-2007 standards. Thus, Prunus sibirica is a novel non-crop feedstock for biodiesel production in China. We have been addressing this issue by studying the regulation of oil accumulation in SASK.Results As part of this research we have carried out a detailed lipidomic analysis in developing SASK. Here, oil contents and fatty acid (FA) compositions were studied in developing SASK from AS-80 and AS-84, at intervals of 1 week from 3 weeks after anthesis (WAA) to 9 weeks. The major differences between the two germplasms are higher contents of C18:1 and C18:2 in AS-80 than in AS-84 at a mature stage. The SASKs of 4, 6 and 8 WAA, respectively representing early, mid and late phases of oil accumulation, were selected as optimal samples for lipidomic analysis. The molecular species in individual lipid classes between AS-80 and AS-84 were similar, and exhibited quite distinct patterns with SASK development. Additionally, the lipidomic data coupled with qRT-PCR analysis suggested that three mechanisms allow the flux of FA through phosphatidylcholine that influenced the molecular composition of eventual TAG.Conclusions The first report on lipidomic analysis during seed development in wood oilseed plants. Our data contribute significantly to understand the underlying mechanisms of lipid accumulation in P. sibirica , and may also present strategies for engineering oil accumulation in oilseed plants.
Exploring the Consequences of Teachers’ Self-efficacy: A Case of Teachers of English as a Foreign Language
Authors: Alibakhshi Goudarz , Nikdel Fariborz , Labafi Akram 
Published: March 2020

Abstract
Teacher self-efficacy, as a socio-affective concept, has been abundantly studied in relation to different factors. However, it seems that the consequences of teachers' self-efficacy have not been appropriately explored to the present date. The present study aimed at investigating the consequences of EFL teachers' teaching self-efficacy. In so doing, a qualitative research method was used. The data were collected through semi-structured interviews with 20 EFL teachers who were selected through purposive sampling. The interviews were content analyzed thematically. Findings showed that self-efficacy has three main consequences: pedagogical, learner-related, and psychological. Each of these consequences has several sub-categories. It can be concluded that high self-efficacy affects teachers’ teaching practices, learners’ motivation, and achievement. It also affects teachers’ burn-out status, psychological being, as well as their job satisfaction. The findings can be theoretically and pedagogically important to EFL teachers, teacher-trainers, and administrators of educational settings.
Borneol promotes apoptosis of human glioma cells by regulating mTORC1 / eIF4E / HIF-1a pathway
Authors: wang zeng , Li Qinglin , Xia Liang , Li Xia , Sun Caixing , Wang Qiong , Cai Xinjun , Yang Guonong 
Published: March 2020

Abstract
The main reason for the failure of malignant glioma treatment is local tumor recurrence. Tumor cells in hypoxic microenvironment activate HIF-1 α transcription, and thus promoting tumor invasion and metastasis is one of the important reasons. In our previous study, we clearly established that borneol opens the blood-brain tumor barrier and its related mechanism. However, the effects of borneol on glioma treatment have not yet been elucidated. Therefore, in this study, we evaluated the effects of borneol on cell proliferation and the expression of HIF-1a, mTORC1, eIF4E were by constructing in vivo SD rat brain glioma model and in vitro human primary cultured glioma cell model. Further, we measured the apoptosis effect and mechanism induced by borneol in human primary cultured glioma cells. We found that borneol could induce primary glioma cells apoptosis by suppressing the mTORC1 / eIF4E / HIF-1 α signaling pathway. Further, we also found that borneol could downregulate the expression of Bcl-2 and upregulation the expression of Bax and caspase-3,suggesting that borneol could further regulate the expression of apoptosis-related factors and induce the apoptosis of glioma cells. In conclusion, the findings of the present study suggest that HIF-1α may be a key factor in apoptosis of glioma cells, and mTORC1 / eIF4E / HIF-1a pathway is involved in the apoptosis induced by borneol in malignant glioma. Our results not only reveal the target and molecular mechanism and action of borneol leading to promote apoptosis in glioma cells, but also provide experimental basis and theoretical support for the clinical application of borneol.
Land-use based modeling approach for determining freshwater nitrate loadings from small agricultural watersheds
Authors: Grizard Pierre , MacQuarrie Kerry T. B. , Jiang Yefang 
Published: March 2020

Abstract
Nitrate released from a variety of land-use activities is a major factor in the degrading conditions observed in many watersheds and estuaries. In this research a spatially lumped model is developed to estimate annual nitrate loads and concentrations from over 100 small watersheds in the Canadian province of Prince Edward Island (PEI). Nitrate source concentrations are associated with major land-use categories, and nitrate attenuation, based on the width of riparian zones, and transport delay due to groundwater residence time are simulated. To investigate the uncertainty of the results, model parameters were selected using a Latin hypercube sampling method. Nitrate concentrations from 12 watersheds were used for model calibration (R2 = 0.91), while 118 other watersheds were used for verification purposes (R2 = 0.82). Overall, the lumped parameter model is shown to be a useful tool for simulating annual nitrate loadings from agricultural watersheds when detailed spatiotemporal agricultural land-use data are available. For PEI the model results indicate that nitrate loadings to estuaries are strongly related to agricultural land, especially the land area in potato production.
Recent Advances of Sulfonylation Reactions in Water
Authors: Wu Li , Peng Lifen , Hu Zhifang , Jiao Yinchun , Tang Zilong 
Published: March 2020

Background:
The sulfonyl groups are general structural moieties present in agrochemicals,
pharmaceuticals, and natural products. Recently, many efforts have been focused on developing efficient
procedures for preparation of organic sulfones.

Materials and Methods:
Water, a proton source, is considered one of the most ideal and promising solvents in
organic synthesis for its easy availability, low cost, nontoxic and nonflammable characteristics. From the green
and sustainable point of view, more and more reactions are designed proceeding in water.

Objective:
The review focuses on recent advances of sulfonylation reactions proceeding in water. Sulfonylation
reactions using sodium sulfinates, sulfonyl hydrazides, sulfinic acids, and sulfonyl chlorides as sulfonating
agents were introduced in detail.

Results and Discussion:
In this review, sulfonylation reactions proceeding in water developed in recent four
yields were presented. Sulfonylation reactions using water as solvent have attracted more and more attention
because water is one of the most ideal and promising solvents in organic synthesis for its facile availability,
low cost, nontoxic and nonflammable properties.

Conclusion:
Numerous sulfonating agents such as sodium sulfinates, sulfonyl hydrazides, sulfinic acid,
sulfonyl chlorides and disulfides are efficient for sulfonylation reactions which proceed in water.
Experimental Study of Therapeutic Efficacy of the Topical Preparation «Kourochitin» in Skin Allergy
Authors: Klimovich Anna Anatolievna , Styshova Olga Nikolaevna , Popov Alexander Mikhailovich , Moskvina Tatiana Vladimirovna , Tsybulsky Alexander Vasilevich , Derunov Dmitry Albertovich , Stonik Valentin Aronovich 
Published: March 2020

Background:
The researchers of PIBOC RAS developed the dermo-protective topical
drug called «Kourochitin», active substance of which is known quinazoline alkaloid tryptanthrin. In
the present work, therapeutic efficacy of this drug in the treatment of allergic dermatosis was evaluated.

Methods:
Dermo-protective action of «Kourochitin» was studied in tow murine models: 2, 4- dinitrofluorobenzene-
induced allergic contact dermatitis (ACD) and imiquimod-induced psoriasis.

Results and Discussion:
In a model ACD, it was shown that «Kourochitin» exhibits the curative
action on pathophysiological, hematological and immunological parameters in ACD. Namely,
«Kourochitin» 1) reduces the level of erythema in the allergen damaged skin area and increases the
healing index of the epidermis; 2) normalizes the content of eosinophils, basophils and monocytes in
the blood of experimental animals; 3) inhibits the production of main pro-inflammatory cytokines:
interleukins - 1 and 2, interferon-gamma, and granulocyte-macrophage colony-stimulating factor. In
a murine model of imiquimod-induced psoriasis, it was shown that «Kourochitin» application led to
reduction in psoriasis severity on the inflamed epidermis of experimental animals. Additionally, in
veterinary research, «Kourochitin»-treatment of canine atopic dermatitis almost completely eliminated
signs of allergic manifestations on the epidermis.

Conclusion:
The obtained data suggest that «Kourochitin» as anti-inflammatory, anti-allergic, and
wound healing remedy is a potential drug for therapy of various dermatological diseases, in particular
allergic skin lesions.
Hydrophilic Interaction Chromatography Analysis of Esculin in Ointments with UV Detection
Authors: Abdulla Fatma H. , Rasheed Ashraf Saad 
Published: March 2020

Background:Esculin (ESCN) is used in the pharmaceutical industry with intravenous effect, stimulant and anti-inflammatory capillaries, like vitamin P. It is a significant component of many anti-inflammatory remedies such as esqusan, esflazid and anavenol [14]. It is also found in numerous other remedies available in the market such as proctosone, anustat, and ariproct.Objective:To determine experimental conditions, to elucidate retention behavior of esculin in HILIC mode. Moreover, to suggest new ways to separate and determinate esculin in ointments.Methods:Two hydrophilic columns were obtained by attaching molecules of sulfobetaine to polystyrene- divinylbenzene particles were studied for chromatographic separation of esculin. The various lengths of the chain are used as an investigative instrument for esculin retention conduct. The change of ACN percentage, buffer concentrations and pH of mobile phase in order to study the retention conduct of esculin.Results:A mixed mode hydrophilic interaction and ion exchange is the separation mechanism of esculin. A calibration graph was created for two columns. The concentration range was 8-1200 ng.ml-1, LOD 2.33 and 1.40, RSD% 0.31-1.02, , LOQ 7.07 and 4.25 ng.ml-1, Erel% 0.83 ± 0.68 and 0.545 ± 0.45, Recovery% 100.86 ± 0.68 and 1054 ± 0.45.Conclusion:The findings of the current study introduced new ZIC-HILIC methods for the separation and quantitation of esculin.
The Effects of Hydroalcoholic Extract from Alhagi on Matrix Metalloproteinase- 9 Production in Peripheral Blood Mononuclear Cells from Patients with Rheumatoid Arthritis
Authors: Ahmadi Mahbouba , Ebrahimi Reyhane , Ansari Mohammad , Akhlaghi Masoumeh , Mahmoudi Mahdi , Amin Gholamreza , Emamgholipour Solaleh 
Published: March 2020

Background:
The up-regulation of matrix metalloproteinase 9 (MMP-9) along with the
imbalanced ratio of MMP-9 to tissue inhibitor of metalloproteinase 1 (TIMP-1) is important in the
pathogenesis of Rheumatoid Arthritis (RA). Here, we investigated whether hydroalcoholic extract
from the root of Alhagi camelorum Fisch can affect the levels of MMP-9 and TIMP-1 in peripheral
blood mononuclear cells (PBMCs) of RA patients.

Objective:
In the current study, we suggest that Alhagi may have an inhibitory effect on MMP-9
production, which is mainly responsible for joint destruction in RA. In addition, we would like to
stress that our findings, along with others, can provide the basis for future studies, which might help
in determining the role of chemical ingredients of Alhagi as therapeutic targets for RA treatment.

Methods:
PBMCs were isolated from 12 RA patients and 12 healthy subjects and treated with two
concentrations of Alhagi extract (100 and 500 μg/ml) for 24 h. MMP-9 gene expression and protein
production, TIMP-1 levels and nitric oxide (NO) production were evaluated using standard methods.

Results:
Alhagi (500 μg/ml) caused a significant reduction in the expression and activity of MMP-9
in PBMCs from healthy (p=0.003 for both of them) and patient (p= 0.05 and p=0.02 respectively)
subjects. Moreover, Alhagi (100 μg/ml) decreased MMP-9 production in the healthy subjects’ group
(p=0.02).

Conclusion:
The present study reveals the inhibitory effects of Alhagi on the production of MMP-9
as the main responsible cause of joint destruction in RA.