bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cloning and Characterization of Partial Chlorophyll a Oxygenase (CAO) Gene Involved in Soybean Shade Tolerance Mechanism
Authors: Khumaida Nurul , Kisman Kisman , Sopandie Didy 
Published: November 2019

Plant tolerance mechanism under shade stress is indicated by high chlorophyll-b content, which is synthesized from chlorophyll a by chlorophyll a-oxygenase (CAO) gene. The study was aimed to clone and characterize the partial of CAO gene involved in shade tolerance mechanism in soybean. Total RNA was isolated from the second trifoliate leaves of soybean plants and the first strand cDNA was prepared using Reverse Transcriptase Moloney Murine Leukemia Virus (RT-M-MLV) (RNase H-). Northern blot hybridization was used to analyze expression of partial CAO gene. The result showed that one partial gene, CAO 3-4 (1,052 bp), comprised of 292 adenine (a), 241 cytosine (c), 276 guanine (g) and 242 thymine (t), therefore the number of amino acid deduction was 338. The expression of CAO 3-4 partial gene was higher in shade tolerant genotypes than in shade sensitive genotypes, both under 50% shade and dark. These results demonstrated that the CAO gene is involved in shade tolerance mechanism of soybean.
The Interaction between Endophytic Actinomycetes and Rhizobium in Leguminous Plants
Authors: Sahur Asmiaty 
Published: November 2019

Biological N2 fixation represents the major source of N input in many agricultural soils including those in arid regions where little artificial fertilizer is applied. The major N2-fixing systems in agriculture are the symbiotic systems, where bacteria such as rhizobia interact with legumes to fix atmospheric nitrogen which plays a significant role in improving the fertility and productivity of low-N soils. The symbiotic association of legume-rhizobium is initiated by the colonization of the rhizosphere by the rhizobia and subsequent attachment to the root hairs of the host plant. Furthermore, the host will produce flavonoids, such as luteolin in alfalfa and diazedin in soybean, which interact with nod protein in the rhizobia. Moreover, this process then elicits the expression of a cluster of nodulation genes such as nod, nol, and noe in the rhizobia. The interaction is potentially of great importance to the health and growth in nature of this nodulating legume.The interaction between endophytic Actinomycetes and rhizobia in leguminous plants is one way to improve the capability of leguminous plants to fix atmospheric nitrogen in plant roots and contribute to the plants nutrition. From other studies, we know that certain types of Actinomycetes, for example Streptomyces, interact with peas to form healthy roots as an effective site to form nodules and improve biological nitrogen fixation. Knowledge about this activity against fungal pathogens might lead to finding biocontrol agents for use in sustainable agricultural practices.Root-colonizing soil borne Actinomycetes might influence root nodulation in leguminous plants by increasing root nodulation frequency, possibly at the sites of infection by Rhizobium spp. Actinomycetes also colonize and sporulate within the surface cell layers of the nodules. This colonization leads to an increase in the average size of the nodules that form and improves the vigor of the bacteroids which generate the red color within the nodules by enhancing nodular assimilation of iron and possibly other soil nutrients. Keywords: symbiotic, biological, nitrogen, molecular interaction
The Induction of Primary and Secondary Somatic Embryo to Support Arabica Coffee Propagation
Authors: Ibrahim Meynarti Sari Dewi , Hartati Raden Roro Sri , Rubiyo Rubiyo , Purwito Agus , Sudarsono Sudarsono 
Published: November 2019

The primary and secondary somatic embryogenesis can be used to propagate Coffea arabica L clonally. However, the success of this propagation was depended on plant growth regulator and varieties. This study aimed to examine the possibility of 2,4-D and thidiazuron application to form primary and secondary somatic embryo to support Arabica coffee clonal propagation. The study consisted of two activities (1) 2,4-D and thidiazuron Application to Induce Primary Somatic Embryogenesis of Arabica Coffee and (2) The Application of thidiazuron in Solid and Semi-Solid Media to Induce Secondary Somatic Embryos. The results indicated significant effect of varieties and plant growth regulator on fresh weight, number of torpedo and germinated embryo. However, it showed no significant effect on callus formation percentage. The best medium to induce primary somatic embryogenesis depending on variety, on the treatment of 4.52 μM 2,4 -D +18.16 μM thidiazuron was the best for AS2K and Sigarar Utang varieties, S 795 at 4.52 μM 2,4-D + 9.08 μM thidiazuron, whereas Kartika at 4.52 μM 2.4-D + 13.62 μM thidiazuron. The morphology of coffee somatic embryo was normal. Primary somatic embryo was developed indirectly, whereas the secondary somatic embryo was directly. The application of 9.08 μM thidiazuron increased the percentage and number of secondary somatic embryos, hence enhancing number of Arabica coffee planlet. Keywords : Coffea arabica L, 2,4-D, thidiazuron, semi-solid media, Indirect somatic embryogenesis
The Study	of Organic Fertilizers Application on Two Soybean Varieties in Organic Saturated Soil Culture
Authors: Aziz Sandra Arifin , Melati Maya , Ramadhani Elrisa 
Published: November 2019

Indonesia is facing soybean shortage and the low productivity of soybean production from decreasing acreage. Saturated soil culture (SSC) could be one of the solutions for marginal land with drainage problem. Saturated soil culture is cultivation technology that gives continuous irrigation and maintains water depth constantly and makes soil layer in saturated condition. Farmers can use on-farm inputs that are normally available at the production site. An organic farming system may be able to ensure local and regional food security through continuous production. The study was conducted at Cikarawang Experimental Station of Bogor Agricultural University, Indonesia, from December 2009 to February 2011. The objective of the research was to determine the influence of organic fertilizer application on the productivity of two varieties of soybean, “Anjasmoro” and “Wilis”, in organic saturated soil culture conducted in two cropping seasons. Prior to the experiment all plots were applied with 2 ton.ha-1 of dolomite, 2 ton.ha-1 of rice hull ash, and 10 ton.ha-1 of chicken manure. The experiment of the first season used split plot design with six replications. The main-plot was organic fertilizer that consisted of chicken manure only (20 ton.ha-1), chicken manure (10 t.ha-1) + Centrosema pubescens (4.2 t.ha-1), chicken manure (10 ton.ha-1) + Tithonia diversifolia (4.2 t.ha-1). The sub-plot was soybean varieties “Anjasmoro” and “Wilis”. The soybean productivity was not affected by the application of organic fertilizers. “Willis” productivity (1.98 t.ha-1) was higher than “Anjasmoro” (1.80 t.ha-1). The experiment of second season used split-split plot design with three replications. The main-plot consisted of 50 and 100% fertilizer rate of the first cropping season; the sub-plot was the same types of organic fertilizer with soybean varieties as sub-sub-plot. Rate of fertilizer, types of organic fertilizer and soybean variety did not affect productivity. Application of 50 and 100% rate of fertilizer in the second season produced 2.41 and 2.55 t.ha-1 of dry seeds, respectively. Soybean plants treated with chicken manure, green manure from C. pubescens and T. diversifolia produced 2.45, 2.50 and 2.49 t.ha-1 of dry seeds, respectively. “Anjasmoro” and “Wilis” produced 2.50 and 2.45 t.ha-1 of dry seeds, respectively. Productivity in the second season was 26.26-36.61% higher than those of the first cropping season.
Water Balance in Oil Palm Plantation with Ridge Terrace and Nephrolepis biserrata as Cover Crop
Authors: Ariyanti Mira , Yahya Sudirman , Murtilaksono Kukuh , Suwarto Suwarto , Siregar Hasril H. 
Published: November 2019

The existence of oil palm plantations as a possible cause of drought in the surrounding areas in Indonesia is a critical issue. Therefore, information related to the effects of oil palm plantations on the surrounding environment in terms of soil water content (SWC) availability is needed. Soil and water conservation techniques in the form of ridge terracing and cover crops, such as Nephrolepis biserrata, can be expected to potentially improve soil water reserves, especially in the dry-season, by accumulating water in the rainy season. This study aimed to study the effects of N. biserrata as cover crop, together with the potential effects of ridge terraces, on the water balance in mature oil palm plantations. The research was conducted in mature oil palm plantations, Afdeling III block 375 (planted in 1996) and block 415 (planted in 2005), Rejosari Unit, PT Perkebunan Nusantara (PTPN) VII in Natar District, South Lampung Regency, Indonesia, from August 2014 to January 2015. The research was based on of setting up 15 m x 20 m experimental plots with the following treatments: (i) without ridge terraces and without N. biserrata (G0T0); (ii) without ridge terraces but with N. biserrata (G0T1); (iii) with ridge terraces but without N. biserrata (G1T0); (iv) with ridge terraces and with N. biserrata (G1T1). Hydrology parameter data were collected for each treatment plot; water balance was calculated using a water balance equation. The results showed that the use of the cover crop N. biserrata in combination with ridge terraces helped improving SWC reserves by approximately 71% and 12%, respectively. The use of N. biserrata as a cover crop reduced the rate of water loss by percolation and run-off, by approximately 36% and 80%, respectively, in an area where the annual rainfall is above 2,400 mm per year. The presence of N. biserrata shortened the period of SWC deﬁcit by extending the period of a water surplus by 70 days when compared with ridge terracing alone (which reduced the period of SWC by 50 days).
Study on Medicinal Plants Used by the Ethnic Mamuju in West Sulawesi, Indonesia
Authors: Syamsiah Syamsiah , Hiola Siti Fatmah , Mu’nisa Andi , Jumadi Oslan 
Published: November 2019

Indonesia is known as a country with very high biodiversity, within which are potential medicinal plants that have not been fully explored or utilized. The potential of this natural biodiversity for the health and welfare of the Indonesian community is regarded as high, if it is properly utilized. An example of the potential benefits of these plants is reflected in the types of traditional medicinal plants used by the Mamuju ethnic in West Sulawesi. This research aims to describe the types of plants used as traditional medicines by the Mamuju ethnic group. The study is based on descriptive approach that used a combination of observations, interviews and taxonomy. The results of the study revealed that there are 31 species of medicinal plants used as sources of traditional medicine by the Mamuju ethnic group, of which there are 33 medical herbs used for the treatment of 31 kinds of diseases. The health issues that are addressed through the use of medicinal plants include the treatment of some types of internal diseases, including cysts, cancer, tumors, high blood pressure, ulcers and diabetes; disease symptoms such as itching, swelling, myopic, new injuries and infections. Some traditional medicines are used in relation to onset of menstruation, and postpartum treatment. The plant components that are used for medicinal purposes include roots, stems, stem bark, leaves, flowers, fruit, seeds, rhizomes and tubers. However, the predominant plant component used for medicinal purposes is plant leaves.
A Unique ‘Chain Tree’ Bauhinia (Caesalpinioideae, Leguminosae) from Pagerwunung Darupono Conservation Park, Central Java, Indonesia
Authors: Lianah Lianah 
Published: November 2019

A tree species having a unique stem morphology has been found to grow at Sam Poo Kong temple, Gunung Batu, Semarang city, and at Pager Wunung Darupono Conservation Park, Central Java, about 30 km away from the temple. Based on plant key reference identification the name of this tree species is Bauhinia scandens Willd, a liana from Leguminosae family. This species has a local name of ‘pohon rantai’ due to the chain shape of the stems. It flowered in August and the fruits matured in October. In vitro germination of the seeds collected from the Conservation Park was not successful and only one out of 30 seeds germinated in vivo after 12 weeks. Further studies should be conducted on conservation and propagation of this unique species.
Radiosensitivity Levels of In Vitro Cultured Celosia cristata Planlets by γ - Ray Irradiation
Authors: Hayati Dhieni , Aisyah Syarifah Iis , - Krisantini 
Published: November 2019

Plantlets of the ornamental plant Celosia cristata were irradiated with gamma rays to increase their genetic diversity. This study was aimed to establish the lethal levels of gamma radiation (LD20, LD30 and LD50) for C. cristata plantlets. The irradiation doses used were 0, 25, 50 and 75 Gy. The growth of irradiated plantlets was evaluated to the third generation. Irradiated C. cristata MV1 plantlets showed a decrease in growth, with plantlets irradiated at 75 Gy showing only 30% survival. Abnormal growth characteristics observed in the third generation plantlets included the shortening of internodes, and curling of leaves. LD50, LD30 and LD20 of C. cristata were 68.73 Gy, 46.68 Gy and 35.65 Gy, respectively.
Characterization of 13 Accessions of Purslane (Portulaca sp.) from Bogor, West Java, Indonesia
Authors: Setiawan Farensa Ikman Dedi , Aisyah Syarifah Iis , Krisantini Krisantini 
Published: November 2019

Characterization is part of crop the breeding process aims to protect biodiversity and preserve local germplasm. Indonesia has two known species of Portulaca, Portulaca oleracea and P. grandiflora. Thirteen Portulaca accessions have been collected from Bogor area, comprising five accessions of Portulaca oleracea and eight accessions of Portulaca grandiflora. This study was aimed at the characterization of the 13 accessions and was undertaken at Indoflower BLST, Bogor, between October 2015 to February 2016. The results showed a significant difference in the color of flowers between the two species of Portulaca. The most significant difference is the overall appearance of P. oleracea species. Character color and shape of the flowers between the P. grandiflora white (GW) and P. oleracea are similar. Significant differences between the species exist in relation to the root systems, but the root systems of accessions within each species are generally similar. The branching characteristics between the two species and among accessions are generally similar, with branches emerging through the base of leaves or nodes. However, there are differences in the leaf characteristics between the two species. This study has provided information on flower characteristics of P. grandiflora and P. oleraceae which will be valuable for future breeding efforts in Portulaca.
Improvement of Shelf Life and Sensory Quality of Pummelos by Fruit Waxing and Wrapping
Authors: Aji Titistyas Gusti , Susanto Slamet , Sukma Dewi , Ardie Sintho Wahyuning 
Published: November 2019

The aims of this study were to evaluate the physical and chemical change of pummelo fruit during storage with waxing and wrapping, and to determine the effect of waxing and wrapping and their its interaction in affecting shelf life of pummelo fruits. This research was arranged in a randomized complete block design with two factors and three replications. The first factor was waxing consisted of without waxing, 10% beeswax, 5% beeswax, and chitosan. The second factor was wrapping consisted of without wrapping and wrapping using either transparent yellow or white plastic wrap. Pummelo fruits experience weight loss, reduced total soluble solid (TSS), decrease in firmness and total acidity during 48 days of storage in room temperature. Waxing using beeswax or wrapping using plastic wraps significantly suppressed weight loss and maintained fruit firmness compared to those without waxing and wrapping. However, waxing and wrapping did not significantly affect the quality of pummelo fruits. Based on the fruit’s weight loss, firmness, and panelist preferences, 5% or 10% bees wax can be used as waxing. Both transparent yellow and white plastic wraps can be used to maintain fruit weight, firmness, and appearances during storage.
Can We Produce True Seed of Shallot (TSS) from Small Size Shallot Sets?
Authors: Palupi Endah R. , Manik Fatiani , Suhartanto M Rahmad 
Published: November 2019

The Indonesian government has been promoting the use of true seed of shallot (TSS) for shallot production due to its higher productivity potentials and greatly reduced risk of disease transfer. This research was conducted at Leuwikopo experimental station, Department of Agronomy and Horticulture, Bogor Agricultural University, West Java, Indonesia, in 2015. The aim of the research was to study TSS production from small size shallot sets “Bima Brebes”, an easy to flower shallot cultivar. The trial was arranged in a completely randomized block design with two factors; BAP concentration i.e. 50, 100 150, 200 ppm as the first factor and the sizes of mother bulbs, i.e. small (3-4 g) and medium (5-10 g), as the second factor. The results showed that the vegetative growth of plants from the two bulb sizes were similar. TSS production and TSS quality from small size (3-4 g) bulbs was also comparable to that of medium size shallot set, except for 1000 seeds weight in which medium size mother bulb produced heavier 1000-seeds weight than that of small size shallot set. Therefore small size shallot set can potentially be useful for TSS production.
Evaluation of Progress Selection of F2 - F6 Population, A Cross between Two Lowland Tomato Genotypes
Authors: Sautomo Mohammad Wahyu , Syukur Muhamad 
Published: November 2019

One of the parameters that can be measured from the activities of selection is progress selection. The population of a plant which has been selected is expected to be good against a derivative of a crop which was selected. The aim of this research is to study the results of progress selection varieties superior of tomato plants down the generations in the population F2 005001 until F6 005001-4-1-12-3 with a standard tomato cultivar, “Ratna”. The result showed that the selected genotypes have shown improvements over the standard cutivar in terms of weight per fruit, fruit weight per plant, the number of fruits per plant, fruit thickness and the age of harvested plants in the population F2-005001 until F6 005001-4-1-12-3. The mean against the character being observed in the population F2 005001 until F6 005001-4-1-12-3 indicated that the result is better if compared with both parents, P1 (SSH-5 ) and P2 (Intan) and the standard cultivar “Ratna”. The value of heritability a wider sense indicate its value being on each character of being selected, while the value of heritability in a more narrow sense showed a low value on every character. The value of progress against character selection of weights per fruit, the weight of the fruit per plant, the number of fruit per plant, thick ﬂesh fruit and the age of harvest showed increased slow progress in a genotype F2 005001 followed by a period of rapid progress in a genotype F4 005001-4-1 and very slow in a genotype F6 005001-4-1-12-3.
Adaptation of Wetland Rice to Extreme Weather
Authors: Dulbari Dulbari , Santosa Edi , Sulistyono Eko , Koesmaryono Yonny 
Published: November 2019

Climate change is believed to increase the intensity and the frequency of extreme weather events in reference to strong winds and heavy precipitations. The extreme event is defined as strong wind at speed of 50 km.h-1 and rain fall intensity 10 to 20 mm.h-1 or more . This condition is detrimental to rice production as this may lead to lodging and flooding which normally occurs during the grain filling stage to harvesting resulting in lower yield and grain quality. The. Simultaneous extreme events and critical rice growth occured more frequently due to increasing cropping season within a year in Indonesia. Therefore, it is important to mitigate and develop adaptation strategies in order to sustain rice production. Efforts to adapt to these extreme environmental conditions are mostly based on genetics and agro ecological approaches. Genetically, rice with strong hills, high aerodynamic with low water retention is desired. Agro-ecological manipulation is conducted through wind break application, planting arrangement to facilitate better sunshine penetration, to manage water level and planting calendar. Availability of weather station in the field is important to improve mitigation and continuous adaptation strategy against extreme weather events. Keywords: plant canopy architecture, climate change, heavy rainfall, lodging, strong wind
A importância da coordenação, acompanhamento e fiscalização no estágio de estudantes
Authors: Reis Jair Teixeira dos , Fauth Gabriela 
Published: November 2019

As exigências profissionais do mercado de trabalho na sociedade contemporânea concorrem para que os diversos cursos superiores e profissionalizantes incluam em seu conteúdo programático atividades teórico-práticas para que os estudantes concluam seus estudos com trabalhos e tarefas cada vez mais complexos, neles envolvendo procedimento que harmonizem a teoria à prática num ambiente empresarial externo.Este artigo tem por objetivo avaliar a importância a ser dada às etapas do estágio de estudante para a formação profissional.para subsidiar o estudo qualitativo, entrevistamos 20 estudantes do ensino superior encaminhados pelos Agentes de Integração a empresas localizadas nos Municípios de Cariacica e Vitória, localizados no estado do Espírito Santo - Brasil. Destes 35% do sexo masculino e 65% do sexo feminino. As idades dos sujeitos variam de 16 a 29 anos. Apurou-se que os centros educacionais, principalmente aqueles voltados para o ensino superior, tem omitido nas suas funções de coordenação e acompanhamento. No decorrer da pesquisa foi possível entender: se as razões das substituições dos trabalhadores empregados por estagiários, a baixa qualidade dos acompanhamentos e avaliações, os motivos que ocasionaram o crescente aumento de estagiários no mercado formal de trabalho e o seu desvirtuamento.