bluuebunny/crossref_metadata_2025_split huggingface dataset:
Learning to count: determining the stoichiometry of bio-molecular complexes using fluorescence microscopy and statistical modelling
Authors: Mersmann Sophia F. , Johns Emma , Yong Tracer , McEwan Will A. , James Leo C. , Cohen Edward A.K. , Grove Joe 
Published: July 2020

Cellular biology occurs through myriad interactions between diverse molecular components, many of which assemble in to specific complexes. Various techniques can provide a qualitative survey of which components are found in a given complex. However, quantitative analysis of the absolute number of molecules within a complex (known as stoichiometry) remains challenging. Here we provide a novel method that combines fluorescence microscopy and statistical modelling to derive accurate molecular counts. We have devised a system in which a given biomolecule is differentially labelled with spectrally distinct fluorescent dyes (label A or B), which are then mixed such that B-labelled molecules are vastly outnumbered by those with label A. Complexes, containing this component, are then simply scored as either being positive or negative for label B. The frequency of positive complexes is directly related to the stoichiometry of interaction and molecular counts can be inferred by statistical modelling. We demonstrate this method using complexes of Adenovirus particles and monoclonal antibodies, achieving counts that are in excellent agreement with previous estimates. Beyond virology, this approach is readily transferable to other experimental systems and, therefore, provides a powerful tool for quantitative molecular biology.The statistical models used in our analysis are available here:https://github.com/sophiamersmann/molecular-counting, the raw data used for molecular counting can be found here: 10.5281/zenodo.3955142.
State-behavior feedbacks suppress personality variation in boldness during foraging in sticklebacks
Authors: MacGregor Hannah E. A. , Cottage Aislinn , Ioannou Christos C. 
Published: July 2020

AbstractConsistent inter-individual variation within a population, widely referred to as personality variation, can be affected by environmental context. Feedbacks between an individual’s personality and state can strengthen (positive feedback) or weaken (negative feedback) individual differences when experiences such as predator encounters or winning contests are dependent on personality type. We examined the influence of foraging on individual-level consistency in refuge use (a measure of risk-taking, i.e. boldness) in three-spined sticklebacks,Gasterosteus aculeatus, and particularly whether changes in refuge use depended on boldness measured under control conditions. In the control treatment trials with no food, individuals were repeatable in refuge use across repeated trials, and this behavioral consistency did not differ between the start and end of these trials. In contrast, when food was available, individuals showed a higher degree of consistency in refuge use at the start of the trials versus controls but this consistency significantly reduced by the end of the trials. The effect of the opportunity to forage was dependent on personality, with bolder fish varying more in their refuge use between the start and the end of the feeding trials than shyer fish. This suggests a state-behavior feedback, but there was no overall trend in how individuals changed their behavior, with some individuals spending more, and others less, time in the refuge area at the end than at the start of the trials. Our study shows that personality variation can be suppressed in foraging contexts and a potential but unpredictable role of feedbacks between state and behavior.
Mild membrane depolarization in neurons induces immediate early gene transcription and acutely subdues responses to successive stimulus
Authors: Rienecker Kira D. A. , Poston Robert G. , Segales Joshua S. , Saha Ramendra N. 
Published: July 2020

AbstractThe transcriptional profile of immediate early genes (IEGs) is indicative of the duration of neuronal activity, but it is unknown whether it affected by the strength of depolarization. Also unknown is whether an activity history of graded potential changes influences further neuronal activity. In this work with dissociated rat cortical neurons, we found mild depolarization – mediated by elevated extracellular KCl – not only induces a wide array of rapid IEGs, but also transiently depresses transcriptional and signaling responses to a successive stimulus. This latter effect was independent ofde novotranscription, translation, calcineurin (CaN) signaling, and MAPK signaling downstream of PKC. Furthermore, as measured by multiple electrode arrays, mild depolarization acutely subdues subsequent spontaneous and bicuculline-evoked activity. Collectively, this work suggests that a recent history of graded potential changes acutely depresses neuronal intrinsic properties and subsequent responses. Such effects may have several potential downstream implications, including reducing signal-to-noise ratio during Hebbian plasticity processes.
Competitive Microtubule Binding of PEX14 Coordinates Peroxisomal Protein Import and Motility
Authors: Reuter Maren , Kooshapur Hamed , Suda Jeff-Gordian , Neuhaus Alexander , Brühl Lena , Bharti Pratima , Jung Martin , Schliebs Wolfgang , Sattler Michael , Erdmann Ralf 
Published: July 2020

AbstractPEX14 functions as peroxisomal docking protein for the import receptor PEX5. For docking, the conserved N-terminal domain of PEX14 (PEX14-NTD) binds amphipathic alpha-helical ligands, typically comprising one or two aromatic residues, of which human PEX5 possesses eight. Here, we show that the PEX14-NTD also binds to microtubular filamentsin vitrowith a dissociation constant in nanomolar range. PEX14 interacts with two motifs in the C-terminal region of human ß-tubulin. At least one of the binding motifs is in spatial proximity to the binding site of microtubules (MT) for kinesin. Both PEX14 and kinesin can bind to MT simultaneously. Notably, binding of PEX14 to tubulin can be prevented by its association with PEX5. The data suggest that PEX5 competes peroxisome anchoring to MT by occupying the ß-tubulin-binding site of PEX14. The competitive correlation of matrix protein import and motility may facilitate the homogeneous dispersion of peroxisomes in mammalian cells.
Coming to America: Iranians' use of Telegram for immigration information seeking
Authors: Nikkhah Sarah , Murillo Angela P. , Young Alyson Leigh , Miller Andrew D. 
Published: July 2020

PurposeThis study examines Iran-US migrants' use of the most popular messaging application in Iran—Telegram—and shows how they use it to manage their migration information practices.Design/methodology/approachThis study took a qualitative observation approach. Over the course of six months, over 80 h of observations were conducted on Iran-US migration-related settings within Telegram.FindingsThis work identifies the information practices that emerge as users seek and share information related to Iran-US migration. Telegram plays a vital role across the immigration stages, predominantly in the pre-migration stage. This work also shows how the constraints and features of Telegram influence users' information sharing and seeking practices.Practical implicationsThe findings support the implication that a social media platform that provides multiple ways to interact is likely to better support niche or unanticipated uses.Originality/valueThis study is the first of its kind to explore Iranian Immigrants information practices in the US. The immigration information practices observed during this study represent a valuable example of end-user appropriation within extraordinary constraints, which may be of use in other information-seeking contexts where dedicated or bespoke tools are impractical or ill-advised.
Associations of obstetrical characteristics and dietary intakes with iron status among pregnant women in Selangor and Kuala Lumpur
Authors: Tan Meng Lee , Chin Yit Siew , Lim Poh Ying , Ahmad Fuzi Salma Faeza 
Published: July 2020

PurposeGlobally, iron deficiency is the most common nutritional deficiency and the most common cause of anaemia during pregnancy. To date, there is lack of established studies investigating factors associated with iron status during pregnancy in Malaysia. Therefore, this study aimed to determine the associations of obstetrical characteristics and dietary intakes with iron status among pregnant women attending selected health clinics in Selangor and Kuala Lumpur.Design/methodology/approachA cross-sectional study was conducted among 436 third-trimester pregnant women aged 18–40 years, from six selected health clinics in Selangor and Kuala Lumpur. Data on obstetrical characteristics were extracted from the antenatal medical records. Dietary intakes were assessed using an adapted semi-quantitative food frequency questionnaire via face-to-face interview. Venous blood samples were collected for the measurement of serum ferritin (SF) using ADVIA Centaur analyser to determine iron status.FindingsApproximately one in four of the pregnant women (25.9%) had iron deficiency, with mean SF of 33.35 ± 25.77µg/l. Factors associated with SF were parity (B = −3.048, p < 0.05), frequency of antenatal care visits (B = 1.456, p < 0.05), haemoglobin (Hb) (B = 3.890, p < 0.05), iron intake (B = 0.010, p < 0.05) and frequency of dietary iron supplementation consumption (B = 3.333, p < 0.05). Hierarchical linear regression analyses revealed that dietary factors were significantly associated with SF, after accounting for the obstetrical characteristics.Originality/valueBesides obstetrical factors, the findings emphasised the importance of dietary factors on iron status during pregnancy. Nutrition consultation service particularly on the sources of iron intake and compliance towards dietary iron supplementation shall be provided to all pregnant women.
Mindfulness training for tourism and hospitality frontline employees
Authors: Johnson Karen R. , Park Sunyoung 
Published: July 2020

PurposeThe purpose of this paper is to explore mindfulness training as a viable intervention for frontline employees in tourism and hospitality as a way to aid in the regulation of emotions and reduce or prevent employee burnout while increasing levels of work engagement.Design/methodology/approachBy reviewing related literature, the authors explored the relationships between mindfulness training and emotional labor, and mindfulness, burnout and work engagement.FindingsThe authors suggested the following propositions: mindfulness training can potentially help to regulate emotional labor of tourism and hospitality frontline employees, and mindfulness training can potentially reduce burnout and enhance levels of work engagement of tourism and hospitality frontline employees.Research limitations/implicationsThis study can guide scholars to initiate empirical research to examine the influence of mindfulness training on diverse outcomes related to tourism and hospitality employees.Practical implicationsThis study can help to improve the awareness of leaders and managers of mindfulness training as an intervention to alleviate emotional exhaustion of frontline employees in tourism and hospitality.Originality/valueThis study provides theoretical insights and useful practical implications for ways to establish a suitable work environment that encourages frontline employees to perform genuine or deep acting while minimizing incidents of surface acting and the consequences of emotional labor.
Lesson study in Dutch initial teacher education explored: its potential and pitfalls
Authors: Schipper Tijmen M. , Goei Sui Lin , Van Joolingen Wouter R. , Willemse T. Martijn , Van Geffen Evelien C. 
Published: July 2020

PurposeThis paper explores the potential and pitfalls of Lesson Study (LS) in Dutch initial teacher education (ITE). This context is examined through data drawn from student-teachers and teacher educators participating in LS.Design/methodology/approachThree case studies of three teacher education institutes in the Netherlands are presented, focusing on student-teachers' learning in two cases and teacher educators' learning in the third case.FindingsThe case studies show that LS in the context of Dutch ITE has high potential. All cases yield clear benefits for working collaboratively as a result of participating in a LS. Student-teachers appreciate the explicit focus in LS on how students learn and teacher educators stress how LS may strengthen their role as “teachers of teachers.” Time, planning arrangements, commitment and a LS facilitator are highlighted as essential conditions for LS application in ITE.Research limitations/implicationsThe three cases address a specific ITE context focusing on different target groups (student-teachers and teacher educators in applied and/or research universities). Consequently, results are explorative regarding Dutch ITE.Practical implicationsThe potential of LS in Dutch ITE is recognized and stressed; this study may act as a catalyst for further and wider application of LS in this context, taking into account possible pitfalls and conditions.Originality/valueThis is one of the first studies exploring the potential of LS in Dutch ITE using both student-teachers' and teacher educators' perspectives.
Success prediction of crowdfunding campaigns: a two-phase modeling
Authors: Silva Lafaiet , Silva Nádia Félix , Rosa Thierson 
Published: July 2020

Purpose
This study aims to analyze Kickstarter data along with social media data from a data mining perspective. Kickstarter is a crowdfunding financing plataform and is a form of fundraising and is increasingly being adopted as a source for achieving the viability of projects. Despite its importance and adoption growth, the success rate of crowdfunding campaigns was 47% in 2017, and it has decreased over the years. A way of increasing the chances of success of campaigns would be to predict, by using machine learning techniques, if a campaign would be successful. By applying classification models, it is possible to estimate if whether or not a campaign will achieve success, and by applying regression models, the authors can forecast the amount of money to be funded.

Design/methodology/approach
The authors propose a solution in two phases, namely, launching and campaigning. As a result, models better suited for each point in time of a campaign life cycle.

Findings
The authors produced a static predictor capable of classifying the campaigns with an accuracy of 71%. The regression method for phase one achieved a 6.45 of root mean squared error. The dynamic classifier was able to achieve 85% of accuracy before 10% of campaign duration, the equivalent of 3 days, given a campaign with 30 days of length. At this same period time, it was able to achieve a forecasting performance of 2.5 of root mean squared error.

Originality/value
The authors carry out this research presenting the results with a set of real data from a crowdfunding platform. The results are discussed according to the existing literature. This provides a comprehensive review, detailing important research instructions for advancing this field of literature.
The more the merrier? The effects of system-aggregated group size information on user's efficacy and intention to participate in collective actions
Authors: Lee Yu-Hao , Littles Carlin 
Published: July 2020

PurposeSocial media platforms are increasingly used by activists to mobilize collective actions online and offline. Social media often provide visible information about group size through system-generated cues. This study is based on social cognitive theory and examines how visible group size on social media influences individuals' self-efficacy, collective efficacy and intentions to participate in a collective action among groups with no prior collaboration experiences.Design/methodology/approachA between-subject online experiment was conducted with a sample of 188 undergraduate participants in a large public university in the United States. Six versions of a Facebook event page with identical contents were created. The study manipulated the group size shown on the event page (control, 102, 302, 502, 702 and 902). Participants were randomly assigned to one of the six conditions and asked to read and assess an event page that calls for a collective action. Then their collective efficacy, self-efficacy and intentions to participate were measured.FindingsThe results showed that the system-aggregated group size was not significantly associated with perceived collective efficacy, but there was a curvilinear relationship between the group size and perceived self-efficacy. Self-efficacy partially mediated the relationship between group size and intentions to participate; collective efficacy did not.Originality/valueThe study contributes to social movement theories by moving beyond personal grievance and identity theories to examine how individuals' efficacy beliefs can be affected by the cues that are afforded by social media platforms. The study shows that individuals use system-generated cues about the group size for assessing the perceived self-efficacy and collective efficacy in a group with no prior affiliations. Group size also influenced individual decisions to participate in collective actions through self-efficacy and collective efficacy.
Impact of climate change on food security in Saudi Arabia: a roadmap to agriculture-water sustainability
Authors: Haque Mohammad Imdadul , Khan Md Riyazuddin 
Published: July 2020

PurposeThe purpose of this paper is to provide a detailed analysis of the trends in temperature and rainfall over the period 1967–2016 (50 years) for the Kingdom of Saudi Arabia and estimate the effect of these climatic changes on major crop production.Design/methodology/approachTo set up an empirical association between crop yields and climatic variables, the study uses a fixed effect regression framework. This approach makes it possible to capture the effects of time-invariant indicators and farmers' independent adaptation strategies in reaction to year-to-year variations in precipitation and temperature.FindingsThe study observes a significant increase in average temperature by 1.9 degrees Celsius in the last 50 years and the greatest increase is noted in the summer. However, there is no significant change in rainfall. The results indicate that a one-degree Celsius increase in temperature reduces crop yields by 7–25%. The results also indicate that rainfall has a positive effect on all the crops. But, rainfall could not offset much of the adverse effects of temperature.Research limitations/implicationsFuture research can focus on the analysis of the climate change impact assessment for different regions in the Kingdom of Saudi Arabia and develop a place-based policy.Originality/valueThe recent initiative to phase out crop production makes the Kingdom of Saudi Arabia entirely rely on imports. This may have little or no impact presently. However, in the future, it is possible that any global shocks on agriculture due to climate change or geopolitical instability will make the situation worse off. It will threaten both food and nutrition security in the Kingdom of Saudi Arabia. Therefore, it is important to study these in the present context to prepare a road map for future food, water and nutrition security.
Reframing the properties, places and crime paradigm: exploring spatiotemporal regime shifts
Authors: DeLisle James R. , Grissom Terry V. , Never Brent 
Published: July 2020

PurposeThe purpose of this study is to explore spatiotemporal factors that affect the empirical analysis of whether crime rates in buffer areas surrounding abandoned properties transferred to a Land Bank that differed among three regimes: before transfer, during Land Bank stewardship and after disposition and whether those differences were associated with differences in relative crime activity in the neighborhoods in which they were located.Design/methodology/approachThis study analyzed crime incidents occurring between 2010 and 2018 in 0.1-mile buffer areas surrounding 31 abandoned properties sold by the Land Bank and their neighborhoods in which those properties were located. Using Copulas, researchers compared concordance/discordance in the buffer areas across the three regime states for each property and approximately matched time periods for associated neighborhoods.FindingsIn a substantial number of cases, the relative crime activity levels for buffer areas surrounding individual sold properties as measured by the Copulas shifted from concordant to discordant states and vice versa. Similarly, relative crime activity levels for neighborhoods shifted from concordant to discordant states across three matched regimes. In some cases, the property and neighborhood states matched, while in other cases they diverged. These cross-level interactions indicate that criminal behavioral patterns and target selection change over time and relative criminal activity. The introduction of Copulas can improve the reliability of such models over time and when and where they should be customized to add more granular insights needed by law enforcement agencies.Research limitations/implicationsThe introduction of Copulas can improve the spatiotemporal reliability of the analysis of criminal activity over space and time.Practical implicationsSpatiotemporal considerations should be incorporated in setting interventions to manage criminal activity.Social implicationsThis study provides support for policies supporting renovation of abandoned properties.Originality/valueTo the best of authors’ knowledge, this research is the first application of Copulas to crime impact studies. As noted, Copulas can help reduce the risk of applying intervention or enforcement programs that are no longer reliable or lack the precision provided by insights into convergent/divergent patterns of criminal activity.
Socio-spatial perspectives on open-plan versus cellular offices
Authors: Sailer Kerstin , Thomas Matt 
Published: July 2020

PurposeThis research provides a new perspective on the long-standing debate of open-plan versus cellular offices. It analyzes the effects of workplace layouts on organizational outputs such as innovation, efficiency and privacy by considering the physical space of an organization alongside its organizational structure. This socio-spatial approach draws on correspondence theory originating from space syntax to understand the potential for unplanned encounters between diverse groups of people.Design/methodology/approachThree different organizations are studied, two open-plan and one cellular office. Floor and seating plans are analyzed to calculate the degree of correspondence between the spatial and conceptual closeness of people. Demands for each organization are derived from semi-structured interviews and publicly available information.FindingsThe three studied organizations present very different degrees of openness toward others in ways that challenge conventional views of cellular and open-plan offices. In each case, the degree of correspondence matches the demands placed on the organization, and hence, providing a relatively good fit between the organization and interior environment.Research limitations/implicationsA larger sample of open-plan and cellular offices would be useful to consider in further research.Practical implicationsManagers can use the concept of correspondence to generate the appropriate degree of unplanned encounters between the right sets of people in order to achieve the best organization-environment fit.Originality/valueThe main innovation of this paper lies in its socio-spatial approach, considering physical space alongside managerial, organizational choices.