bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characteristic of ITS fragment of rDNA of Rhodotorula mucilaginosa isolated from gastrointestinal tract of centenarian
Authors: Polishchuk L. V. , Tkachenko K. S. , Fomyna M. A. 
Published: July 2020

Aim. The aim of the work was to analyse the primary structure of the ITS region of the ribosomal operon of the red yeast isolate Rhodotorula mucilaginosa S1 from the gastrointestinal tract of healthy centenarian compared to the available homological basidimycetous nucleotide sequences. Methods. In silico comparison of the ITS nucleotide sequences using NCBI server resources (BLAST programs and "Nucleotide" database) with the reference ITS-fragment of red yeast isolate was performed. Results. Almost 90 % of highly homologous to the reference ITS-fragment out of 800 selected sequences represented genera Rhodotorula and Rhodosporidium with the top hits for R. mucilaginosa strains PMM08-1716L, 3942L and 3684L. The most frequent nucleobases mismatches of ITS-fragments of GenBank’s R. mucilaginosa strains compared to the reference one were observed in the positions 541 (T/C) for 85 % of strains, and 129 (T/C) and 158 (G/A) for 26 % of strains. Conclusions. The primary structure of studied ITS region showed 100 % homology to R. mucilaginosa strains also isolated from humans and animals, as well as the most frequently mismatches for 129th, 158th and 541st nucleotides located in the spacers regions of the ribosomal operon.
Keywords: ITS, rDNA, nucleotide sequence, red yeast, human GIT.
Cardiac deletion of α-E-catenin leads to redused expression of the main component of desmosomes – γ-catenin
Authors: Balatskyi V. V. , Matsevych L. L. , Piven O. O. 
Published: July 2020

Aim. In our present work, we have addressed to the γ-catenin, known main component of desmosomes, expression in hearts with heterozygous and homozygous knockout of α-E-catenin gene. Methods. Alpha-E-catenin conditional knockout mice were bred with α-MHC-Cre transgenic mice. We analyze expression of γ-catenin with real time qPCR and Western blot. Results. Cardiac α-E-catenin deletion leads to downregulation of γ-catenin mRNA and protein levels only in homozygous mice, while we not observed any perturbation of γ-catenin expression in heterozygous mice. Conclusions. We have shown that homozygous knockout of α-E-catenin gene in embryonic heart occur reduction of the main component of desmosomes – γ-catenin mRNA and protein level of expression, which can lead to disruption of the desmosomes structure in adult myocardium.
Keywords: α-E-catenin, heart failure, γ-catenin.
The frequency of terminal deletions sY160 among men with microdeletions of AZFc region Y-chromosome
Authors: Haiboniuk I. Ye. , Tyrkus M. Ya. , Makukh H. V. 
Published: July 2020

Aim. Determine the frequency of absence marker terminal deletions sY160 among men with microdeletions of AZFc region Y-chromosome. Methods. DNA from probands blood samples was isolated using a modified salting out method. Microdeletions of Y chromosome AZF region were analyzed using two multiplex PCR. The molecular-genetic study of terminal deletions (absence of sY160) was carried out using allele-specific PCR and analysed by electrophoresis in a 2 % agarose gel. Results. Among infertile men (1500 individuals), Y chromosome microdeletions were detected in 7% males: microdeletions of AZFa subregion in 1 %, AZFb subregion – 3 %, AZF(b+c) subregions – 15 %, AZFc subregion – 67 %. The presence of heterochromatin marker sY160 was confirmed in 39 cases (84.8 %) and absence of sY160 in 7 men (15.2 %). Absence of sY160 was detected in 2 men with AZFc microdeletion and in 5 men with AZFb+AZFc microdeletions. It is important to point out that terminal AZFc deletion was confirmed in 83.3 % of cases of AZFb+c microdeletions and only in 5.1 % of isolated AZFc microdeletions. Conclusions. Thus, among 15.2 % man with different AZF microdeletions of Y-chromosome the heterochromatin marker of terminal deletion sY160 was absents. The implementation of testing of marker of terminal deletion – sY160 may help to determine if the deletion corresponds to the b2/b4 pattern and to avoid biopsy in man which most likely not benefit from the surgical procedure.
Keywords: male infertility, spermatogenesis, Y chromosome, AZF region, terminal deletions.
Distribution of allelic variants of genes inhibitors and activators ectopic calcification in patients with acute coronary syndrome
Authors: Harbuzova V. Yu. , Obukhova O. A. , Rozumenko I. O. , Dubovyk Ye. I. , Oleshko T. M. , Harbuzova Ye. A. , Shvachko D. V. , Ataman O. V. 
Published: July 2020

Aim. The study of the distribution of polymorphic variants K121Q (gene ENPP1), T134967G (gene ANKH) and A69314G (gene TNAP) is in patients with acute coronary syndrome. Methods. Venous blood of 118 patients with ACS and 110 persons of control group were genotyped for the polymorphism by PCR and restriction fragment length polymorphism method. Results. There is an association between acute coronary syndrome and polymorphic variants of genes TNAP (A69314G) and ANKH (T134967G). ACS risk in carriers of the minor allele of A69314G polymorphism to 2.2 times (P = 0.013; OR = 2.244), and for T134967G polymorphism to 1.9 times (P = 0.024; OR = 1.857) higher than homozygotes for the major allele. Conclusions. Allelic polymorphism gene activators and inhibitors of ectopic calcification is an important factor in hereditary susceptibility to sclerotic lesions of arteries and their complications.
Keywords: gene polymorphism, calcification, acute coronary syndrome.
Relative telomere length of the residents of Lviv oblast
Authors: Huleiuk N. L. , Tyrka M. 
Published: July 2020

Aim. Telomeres are short, tandem repeats of DNA that cap linear chromosome ends by binding members of the shelterin protein complex to form protective telomere loops. An insufficient number of telomere repeats leads to chromosome uncapping, cell senescence, and death. Aim of this thesis is the analysis of relative telomere length (RTL) in whole blood among residents of Lviv region. Methods. The RTL in the whole blood cells was measured in 86 residents of various age (47 men and 39 women aged 18–72) using quantitative real-time PCR (Cawthon’s method). It is based on the simultaneous amplification of telomeric repeats (T) and a single copy gene (S). Results. There is a tendency to decrease RTL with age. The low correlation between RTL and age can be linked to various factors, including the heterogeneity of telomere length at birth, chronic socioeconomic stress, genetic determinism, sensitivity to exogenous pressures. Women and men did not differ significantly in the rate of RTL shortening. Conclusions. The results indicate the absence of reliable differences relative telomere length in individuals of different sexes. The low correlation between RTL and age can be linked to various factors, including insufficient sample size of people aged over 50 years. So in the future we plan to continue these studies in older people.
Keywords: relative telomere length, RT-PCR, age, gender.
Results of mutation status gene HER-2/NEU in patients with breast cancer in Ukraine
Authors: Klimuk B. T. , Dugan O. M. , Stefanovych A. V. , Klymenko S. V. 
Published: July 2020

Aim. Detection of changes in epidermal growth factor receptor 2 (HER-2/Neu) in tumor cells is critical to patient prognosis, prediction of response to standard chemotherapy, and perspectives for the treatment of anti-HER-2 therapy. Methods. The research study was conducted according to the recommendations of ASCO/CAP 2013 to diagnose cases of determining gene expression HER-2/Neu by FISH. To identify the exact status of the gene HER-2/Neu TP53 marker was selected. Results. A statistically significant difference (χ2 = 3.36, p>0.05) was found among the questionable cases of mutated HER-2 / neu in the patient groups which was surveyed according to 2007 and 2013 years recommendations. Conclusions. In comparison with 2007 ASCO/CAP 2007 (from 1.4 % to 4.2 %) 2013 ASCO/CAP showed more cases which was classified as doubtful during the test with two-color breakdown HER-2/CEP17.
Keywords: HER-2/Neu, mutation status, FISH, centromere area, TP53.
Allocation features and frequency of KIR-genes in women with regular early reproductive losses
Authors: Terpyliak O. I. , Zastavna D. V. , Helner N. V. , Osidach S. V. 
Published: July 2020

Aim. KIR (killer cell immunoglobulin-like receptors) are the transmembranous glycoprotein receptors, role of which is to activate or inhibit the functional activity of cells, the surfaces of which they are located on. Genetically determined balance between KIR-activating and KIR-inhibiting receptors can contribute to some diseases predisposition. In this work we have studied the spectrum and frequency of KIR-genes among women with regular early idiopathic reproductive failures. Methods. Gene typing was conducted by PCR-SSP method. Results. Typing of KIR-genes was conducted in 31 DNA samples, extracted from peripheral blood cells from women without reproductive losses and in 38 DNA samples, extracted from peripheral blood cells from women with early reproductive losses of idiopathic nature. We found, that in women with reproductive failures genotypes of KIR-genes were characterized by decreased amount of activating genes. The presence of 2DL3 and 2DL5 genes increases the risk of early reproductive failures in 2 and 3 times, respectively. Conclusions. The shift of repertoire of KIR-genes into the domination of inhibitory genes and the spectrum features of KIR-genes can increase the risk of early reproductive failures.
Keywords: KIR-genes, recurrent pregnancy loss, PCR-SSP.
The contribution of chromosomal abnormalities in the formation of sporadic and recurrent early reproductive losses
Authors: Tkach I. R. , Huleiuk N. L. , Zastavna D. V. , Bezkorovaina G. M. , Fedyshyn T. V. 
Published: July 2020

Aim. The aim of the present study was to investigate the contribution of numerical chromosomal imbalances in products of conception from sporadic pregnancy loss (SPL) and recurrent pregnancy loss (RPL). Methods. Banding cytogenetic and interphase mFISH with the probe panel for chromosomes 13, 14, 15, 16, 17, 18, 21, 22, X and Y. Results. Cytogenetic studies of 419 spontaneously aborted fetuses were performed. The results were stratified in 3 groups according to anamnesis: Group I and II – SPL, Group III – RPL. The contribution of chromosomal aberrations was higher in the genesis of SPL (39.9 %) compared to RPL (27.1 %). Among most often diagnosed chromosomal change was triploidy – 27.19 % in SPL vs 27.78 % in RPL, monosomy X – 21.93 % vs 22.22 % and trisomy 16–18.42 % vs 19.44 %. Conclusions.Consequently, detection of chromosomal aneuploidies in samples from products of conception plays a key role to find out about reasons of reproductive failure in humans.
Keywords: sporadic pregnancy loss, recurrent pregnancy loss, banding cytogenetic, interphase multicolor fluorescence in situ hybridization (mFISH), chromosome abnormalities.
Verification allelic polymorphism's of genes MTHFR and MTR in patients with psoriasis
Authors: Fedota A. M. , Roshcheniuk L. V. , Tyzhnenko T. V. , Admakina A. V. , Horaichuk I. V. , Vorontsov V. M. 
Published: July 2020

Aim. Analysis of single nucleotide polymorphisms C677T, A1298C and A2756G of MTHFR and MTR one-carbon metabolism genes in patients with various forms of psoriasis in Ukrainian population. Methods. A molecular genetic analysis of 77 patients with vulgaris and arthropathic psoriasis by PCR-RFLP was carried out. Results. In patients with vulgaris and arthropathic psoriasis analysis of the distribution of frequencies of genotypes showed a statistically significant difference between them for C677T polymorphic variants. In patients with psoriasis analysis of genotype distribution of series in the two genes as a whole, showed a statistically significant difference between the theoretically expected and actual frequencies for single nucleotide polymorphisms A1298T and A2756G of MTHFR and MTR genes. Conclusions. Among patients with arthropatic psoriasis, which is the most severe form of psoriasis, the homozygotes for the wild-type allele G of A2756G of MTR gene are more rarely, homozygotes of the TT of C667T of MTHFR gene are found more common than among psoriasis vulgaris patients, which may indicate the contribution of other genes to the development of arthropatic psoriasis.
Keywords: psoriasis, arthropatic psoriasis, folate cycle, MTHFR gene, MTR gene.
Features of the molecular expression of alkaline phosphatase gene in chronic myeloid leukemia
Authors: Shvachko L. P. , Kravchuk I. V. , Teleheiev G. D. 
Published: July 2020

Aim. To study expression of alkaline phosphatase gene in chronic myeloid leukemia. Methods. Short-term primary cell culture, real-time PCR. Results. We have investigated the aberrant expression type ALP in CML progression. We have found that the intestinal type ALP is switched to germinal type embrional-like ALP in the patients with clinical CML progression (n=8) resulted from the sequencing analyse. Moreover, we have shown the interconnected epigenetic regulation of aberrant embrional-like type ALP and G-CSFR (granulocyte colony stimulation factor receptor) on the level of mRNA gene expression in CML patients under sodium butirate (HDACi) culture blood treatment (10 мМ). We have revealed simultaneously the epigenetic down-regulation mRNA gene expression of aberrant germinal GALP and up-regulation of G-CSFR by sodium butirate in CML progression. Conclusions. On the data obtained have been concluded that sodium butirate targeted induces the hemopoietic differentiation blood cell potential in CML pathogenesis by the G-CSF signaling pathway activation.
Keywords: CML, alkaline phosphatase, G-CSFR (granulocyte colony stimulation factor receptor), sodium butirate.
The action of extracts of oyster mushrooms and plant collection on survival and the average life expectancy of irradiated mice
Authors: Shamilov E. N. , Abdullaev A. S. , Azizov I. V. 
Published: July 2020

Aim. The aim of this work was to evaluate the effect of extracts obtained from oyster mushroom (Pleurotus ostreatus) and vegetable collection, consisting of marigold flowers (Calendula officinalis), St. John's wort (Hypericum perforatum) and yarrow (Achillea millefolium) on survival and the average life expectancy of irradiated mice. Methods. Medicinal plants included in the collection and of oyster mushrooms were dried, and were obtained the extract of water-alcohol mixture. Extracts vnutribryushinno was administered to mice at a concentration of 250 mg/kg 30 min before irradiation at a dose of 9 Gy. Results. It is found that the introduction of the extract of the oyster mushroom there is a significant increase of life expectancy of lethally irradiated animals, although the survival rate after 30 days is slightly inferior to that of the introduction of the radioprotector of the standard. In the case of a complex extract also increase the duration of life of irradiated animals, however, the percentage of survival after the end of the experiment – 30 days – less oyster extract. Conclusions. The data obtained indicate the prospects of using oyster extract separately and in com-plex with extracts of calendula, St. John's wort and yarrow in the development of pharmaceutical compositions intended for the prevention of local radiation injuries.
Keywords: mouse, survival, irradiation, extract.
Tahmima Anam’s A Golden Age: A Family Saga of Love, Duty and Identity against the Backdrop of War
Authors: Moniruzzaman Miah Mohammad 
Published: July 2020

The paper explores love and duty as the dominant themes in Tahmima Anam’s debut novel A Golden Age (2007). At various points of the story, it seems that both love and duty come at the forefront in turn overshadowing the significance of the other. However, it is not hard to notice that the yearning of love and the call of duty almost equally pervade the story. While analyzing these compelling themes, the paper critically examines how Anam aesthetically mediates the conflict between the immediacy of love and the urgency of duty at a time of armed national struggle. It also emphasizes the status of women in pre-independence Pakistan era male dominated Bengali society and their struggle against various misogynistic norms portrayed through the defiant activities of the female protagonist in the novel. Apart from defending her feminine identity against the odds of contemporary androcentric society, the paper also focuses on the issue of the protagonist’s non-native past and tends to establish her Bangladeshi national identity even though she comes from a different ethnic, lingual and cultural background.
Does state self‐control depletion predict relationship functioning and partner aggression? An ecological momentary assessment study of community couples
Authors: Testa Maria , Wang Weijun , Derrick Jaye L. , Crane Cory , Leonard Kenneth E. , Collins R. Lorraine , Hanny Courtney , Muraven Mark 
Published: July 2020

AbstractIntimate relationship functioning depends upon the ability to accommodate one's partner and to inhibit retaliatory and aggressive impulses when disagreements arise. However, accommodation and inhibition may be difficult when self‐control strength is weak or depleted by prior exertion of self‐control. The present study considered whether state self‐control depletion prospectively predicts male and female self‐reports of anger with partner and arguing with partner. Consistent with the I3 Model (Finkel, 2014, Adv Exp Soc Psychol, 49, 1–104), we also considered whether the association between elevated anger and arguing (i.e., instigation) and partner aggression was stronger when state self‐control (i.e., inhibition) was depleted or among people high in negative urgency. In this ecological momentary assessment (EMA) study, heavy drinking married and cohabiting heterosexual couples (N = 191) responded to three randomly signaled reports each day for 30 days. Depletion predicted anger and arguing with partners both cross‐sectionally and prospectively for men and women. However, after controlling for prior levels of anger and arguing, these effects were diminished, and supplemental analyses revealed that anger and arguing with partner predicted subsequent depletion. Anger and arguing were strongly associated with concurrent reports of partner aggression perpetration and victimization (verbal and/or physical). However, neither state self‐control depletion nor negative urgency moderated these effects. Overall, results suggest a modest impact of depletion on daily couple functioning as well as a potential cyclical effect of arguing on depletion.
SAM Functionalized ZnO Nanowires for Selective Acetone Detection: Optimized Surface Specific Interaction Using APTMS and GLYMO Monolayers
Authors: Singh Mandeep , Kaur Navpreet , Drera Giovanni , Casotto Andrea , Sangaletti Luigi , Comini Elisabetta 
Published: July 2020

AbstractIn modern days, self‐assembled monolayer (SAM) functionalized surfaces represent an interesting tool for the development of ultrasensitive and selective sensing platforms for the detection of chemical substances such as biomolecules and gases. The ability of SAM to generate different functional groups on a single surface such as zinc oxide (ZnO) can be used to immobilize biomolecules and detect different analytes such as gases, proteins, etc. Herein, SAM functionalized ZnO NW‐based sensors are developed for acetone exhaled breath analysis. ZnO NWs are synthesized using a vapor–liquid–solid mechanism and their functionalization is done with two different SAMs, i.e., (3‐aminopropyl)trimethoxysilane (APTMS) and 3‐glycidoxypropyltrimethoxysilane (GLYMO). The enhancement in the electron depletion layer resistance (and also width) due to the capturing of electrons from the ZnO NWs surface by APTMS and GLYMO molecules is found to be the major reason in their superior sensing performances. The amine (–NH2) groups of APTMS monolayer enhance the sensors selectivity toward acetone due to their reactions with acetone molecules, which produce imine in addition to water molecules. Moreover, after the functionalization with APTMS SAMs, the detection limits of the sensors are improved from 6 to 0.5 ppm, which makes these devices potential candidates for acetone exhaled breath analysis.
Integrated process design, scheduling, and model predictive control of batch processes with closed‐loop implementation
Authors: Burnak Baris , Pistikopoulos Efstratios N. 
Published: July 2020

AbstractSimultaneous evaluation of multiple time scale decisions has been regarded as a promising avenue to increase the process efficiency and profitability through leveraging their synergistic interactions. Feasibility of such an integral approach is essential to establish a guarantee for operability of the derived decisions. In this study, we present a modeling methodology to integrate process design, scheduling, and advanced control decisions with a single mixed‐integer dynamic optimization (MIDO) formulation while providing certificates of operability for the closed‐loop implementation. We use multi‐parametric programming to derive explicit expressions for the model predictive control strategy, which is embedded into the MIDO using the base‐2 numeral system that enhances the computational tractability of the integrated problem by exponentially reducing the required number of binary variables. Moreover, we apply the State Equipment Network representation within the MIDO to systematically evaluate the scheduling decisions. The proposed framework is illustrated with two batch processes with different complexities.
Estimating resting energy expenditure from dual‐energy X‐ray absorptiometry: A cross‐sectional study in healthy young adults
Authors: Venturini Ana Cláudia Rossini , Silva Analiza Mónica , Abdalla Pedro Pugliesi , dos Santos André Pereira , Borges Franciane Goes , Alves Thiago Cândido , Siqueira Vitor Antonio Assis Alves , da Cruz Alves Natália Maíra , Ferriolli Eduardo , Coelho Eduardo Barbosa , Duncan Michael , Mota Jorge , Machado Dalmo Roberto Lopes 
Published: July 2020

AbstractObjectiveTo qualify a 3C approach model of dual‐energy X‐ray absorptiometry (DXA) to estimate multicomponent resting energy expenditure (REE) referenced by indirect calorimetry (IC).MethodsA sample of 155 college students, of both sexes (18‐30 years old) was evaluated. Anthropometric measures, REE by IC, and whole‐body DXA‐scans were performed. The REE for each body component was determined after transforming the components from the molecular (DXA) to the organ tissue level. Bland‐Altman and proportional bias analyses were used to verify agreement between REE measured (REEIC) and estimated (REEDXA).ResultsStatistically significant differences were found for all sex comparisons (P < .001), except for age (P = .950). Differences from the final sex‐specific models' were not found between REEIC and REEDXA (P > .05). Men also presented greater expenditure (P < .001) in each component, except for adipose tissue. The plots confirmed the validity of the model for both sexes, with low difference values between the measured and estimated REE. The mean of the differences of REEIC and REEDXA showed heteroscedasticity of the data for men (P = .004). The same error tendency was not evident for women (P = .333).ConclusionsThis 3C model, estimating REE from a multicomponent approach, allows a new application of DXA as tool for understanding intraindividual differences in terms of the mass of metabolically active tissue. Sex and populational differences should be taken in account. Consequently, we present qualified sex‐specific DXA models that can be applied in different contexts such as health and sports, besides considering interpersonal differences in terms of energy expenditure.