bluuebunny/crossref_metadata_2025_split huggingface dataset:
Revisão integrativa: intersectando concepções de educação ambiental com referencial teórico marxiano
Authors: Machado Andreia de Bem , Santos João Vicente Alfaya dos 
Published: June 2020

O objetivo deste artigo é analisar à luz da revisão sistemática o conceito de educação ambiental intersectando concepções de educação ambiental com o referencial teórico marxiano. Para tanto, realizou-se a análise bibliométrica a partir de uma busca sistemática na base de dados Scopus. Como resultado, identificou-se que a pesquisa emerge da Medicina; Ciências Sociais; Ciência Ambiental; Ciências Agrárias e Biológicas; Energia; Bioquímica, Genética e Biologia Molecular; Psicologia; Engenharia; Artes e Humanidades; Enfermagem; Economia, Econometria e Finanças; Ciências da Terra e Planetárias; Profissões da Saúde; Matemática; Negócios, Gestão e Contabilidade; Química; Multidisciplinar; Imunologia e Microbiologia; Ciências da decisão; Farmacologia, Toxicologia e Farmacêutica; Física e Astronomia; Veterinária; Ciência da Computação; Ciência dos Materiais; Neurociência; Engenharia Química e Odontologia. A análise mostrou que embora a preocupação ambiental esteja nas mais diversas áreas, a contribuição do pensamento marxiano ainda não assimilada, mesmo entre aqueles que se dedicam à temática da educação ambiental em uma perspectiva “crítica”. O que se constatou é que a questão ambiental e as propostas de educação ambiental são tratadas com viés idealista (mudança de valores, de comportamentos individuais) e politicista, relegando ao Estado e à vontade política o papel decisório sobre essa temática.
Obra-aula: processos, procedimentos e criação de uma artistagem docente
Authors: Rodeghiero Thiago Heinemann , Rodrigues Carla Gonçalves 
Published: June 2020

Este artigo versa sobre a contribuição que a arte contemporânea pode dar à docência. Objetiva-se evidenciar a possibilidade de uma Obra-Aula através da produção artística do pesquisador e como esta se relaciona com a artistagem (CORAZZA, 2013). Ao perceber os vazamentos que a arte contemporânea ocasiona à educação, mostra-se os encontros e experimentações com essas duas áreas. Delineia-se aqui um plano de consistência nas fronteiras borradas da filosofia, da arte e da educação. Nas Filosofias da Diferença de Gilles Deleuze e Félix Guattari (2010, 2011a, 2011b, 2015) encontra-se potência para manifestar as transformações dos significados hegemônicos incrustados nos significantes e nos signos. Partindo desse desenho monta-se um agenciamento, responsável por evidenciar os picos de fuga das relações estabelecidas. Procura-se referências de arte no fazer junto de Allan Kaprow (2003, 2004, 2010), nas sensações de Kazimir Malevich (GIL, 2010) e na zona invisível de Marcel Duchamp (PAZ, 2014), orientação para a prática artística do pesquisador na qual a representação de uma intenção em obra é rompida. O artigo se desenha em um método que se monta fazendo. Um passarinhar à moda de uma cartografia (DELEUZE; GUATTARI, 2011a) que voa por um território encontrando forças passarinhar. Pousando sobre os artistas referência e bicando frutos poéticos, ele canta uma Obra-Aula que faz melodia com a artistagem docente (CORAZZA, 2012). Uma maneira de justapor elementos variados para pensar o impensado, bem como flautear o inflauteável.
Streptococci and the complement system: interplay during infection, inflammation and autoimmunity
Authors: Syed Shahan , Viazmina Larisa , Mager Riccardo , Meri Seppo , Haapasalo Karita 
Published: June 2020

Streptococci are a broad group of Gram‐positive bacteria. This genus includes various human pathogens causing significant morbidity and mortality. Two of the most important human pathogens are Streptococcus pneumoniae (pneumococcus) and Streptococcus pyogenes (group A streptococcus or GAS). Streptococcal pathogens have evolved to express virulence factors that enable them to evade complement‐mediated attack. These include factor H‐binding M (S. pyogenes) and pneumococcal surface protein C (PspC) (S. pneumoniae) proteins. In addition, S. pyogenes and S. pneumoniae express cytolysins (streptolysin and pneumolysin), which are able to destroy host cells. Sometimes, the interplay between streptococci, the complement, and antistreptococcal immunity may lead to an excessive inflammatory response or autoimmune disease. Understanding the fundamental role of the complement system in microbial clearance and the bacterial escape mechanisms is of paramount importance for understanding microbial virulence, in general, and, the conversion of commensals to pathogens, more specifically. Such insights may help to identify novel antibiotic and vaccine targets in bacterial pathogens to counter their growing resistance to commonly used antibiotics.
Paclitaxel induces apoptosis through the TAK1–JNK activation pathway
Authors: Yu‐Wei Di , Li Zhuo‐sheng , Xiong Shu‐min , Huang Ge , Luo Yan‐fei , Huo Tie‐ying , Zhou Mao‐hua , Zheng You‐wei 
Published: June 2020

Paclitaxel (PTX) has previously been used to treat tumours of various tissue origins, such as lung, breast, ovarian, prostate cancers and leukemia. PTX‐induced apoptosis is associated with p38 mitogen‐activated protein kinase (p38 MAPK), extracellular signal‐regulated kinase (ERK), nuclear factor‐kappa B (NF‐κB) and c‐Jun N‐terminal kinase or stress‐activated protein kinase (JNK/ SAPK) pathways. Transforming growth factor‐beta‐activated kinase 1 (TAK1) and TAK1‐binding protein 1 (TAB1) play an important role in cell apoptosis through the p38, ERK, NF‐κB and JNK signal transduction pathways. To investigate the role of TAK1 in PTX‐induced cell apoptosis, we treated HEK293 and 8305C cells with 0–20 µM PTX for 6, 12 or 24 h. To investigate whether TAK1 can cooperate with PTX for cancer treatment, we transfected cells with TAK1, TAB1 or control plasmid and treated them with PTX (3–10 µM) for 9–24 h. Apoptosis rates were analysed by flow cytometry (Annexin V/PI). Endogenous TAK1 and TAB1, caspase‐7 cleavage, poly ADP‐ribose polymerase (PARP) cleavage, Bcl‐xL level, phospho‐p44/42, phospho‐JNK and phospho‐p38 were detected by western blot. We show that in HEK293 and 8305C cells, PTX enhanced the endogenous TAK1/TAB1 level and induced cell apoptosis in a dose‐ and time‐dependent manner. Upon TAK1 overexpression in HEK293 cells treated with PTX, apoptosis rate, JNK phosphorylation and PARP cleavage increased contrary to heat‐shocked or untreated cells. CRISPR editing of the tak1 gene upon PTX treatment resulted in lower phospho‐JNK and PARP cleavage levels than in cells transfected with the control or the TAK1‐ or TAB1 + TAK1‐containing plasmids. TAK1‐K63A could not induce JNK phosphorylation or PARP cleavage. We conclude that PTX induces HEK293 and 8305C cell apoptosis through the TAK1–JNK activation pathway, potentially highlighting TAK1’s role in chemosensitivity.
Advanced Theragenerative Biomaterials with Therapeutic and Regeneration Multifunctionality
Authors: Chen Baoding , Xiang Huijing , Pan Shanshan , Yu Luodan , Xu Tianming , Chen Yu 
Published: June 2020

AbstractAll tissues and organs can be affected by diseases, and treatments for these diseases can cause damage to surrounding healthy tissues and organs. Therefore, treatment is required that involves disease therapy alongside tissue/organ regeneration. The design, construction, and biomedical applications of biomaterial platforms with both disease‐therapeutic and tissue‐regeneration multifunctionalities are in demand, which are herein referred to as theragenerative (abbreviation of therapy and regeneration) biomaterials. Due to the rapid development of theragenerative biomaterials in versatile biomedical applications, this progress report aims to summarize, discuss, and highlight the rational construction of distinctive theragenerative biomaterials with intrinsic therapeutic performance and tissue‐regeneration bioactivity. Based on the intrinsic response to either external physical triggers (e.g., photonic response or magnetic‐field response) or endogenous disease microenvironments (e.g., mild acidity or overexpressed hydrogen peroxide) and tissue‐regeneration bioactivity, these theragenerative biomaterials are extensively explored in various biomedical fields, including bone‐tumor therapy/regeneration, bone antibacterial therapy/regeneration, skin‐tumor therapy/regeneration, skin antibacterial therapy/regeneration, breast‐tumor therapy/adipose‐tissue regeneration, and osteoarticular‐tuberculosis therapy/bone‐tissue regeneration. The challenges faced and future developments of these distinctive theragenerative biomaterials are discussed, as are methods for further promoting their clinical translation.
An Oxidation‐Enhanced Magnetic Resonance Imaging Probe for Visual and Specific Detection of Singlet Oxygen Generated in Photodynamic Cancer Therapy In Vivo
Authors: Deng Kai , Wu Bo , Wang Cai‐Xia , Wang Qian , Yu Hui , Li Jia‐Mi , Li Kun‐Heng , Zhao Hong‐Yang , Huang Shi‐Wen 
Published: June 2020

AbstractSinglet oxygen is regarded as the primary cytotoxic agent in cancer photodynamic therapy (PDT). Despite the advances in optical methods to image singlet oxygen, it remains a challenge for in vivo application due to the limited tissue penetration depth of light. Up to date, no singlet oxygen‐specific magnetic resonance imaging (MRI) probe has been reported. Herein, a T2‐weighted MRI probe is reported to visually detect singlet oxygen generated in PDT in vitro and in vivo. The MRI probe Ce6/Fe3O4‐M is constructed by co‐encapsulation of photosensitizer Ce6 and Fe3O4 nanoparticles in mPEG2000‐TK‐C16 micelles. Thioketal (TK) linker in the probe is highly sensitive to singlet oxygen, but lowly sensitive to other reactive oxygen species (ROS) existing in physiological and pathological environments. Singlet oxygen, generated with light irradiation, triggers the cleavage of TK, which leads to loss of surface polyethylene glycol, increment of the hydrophobicity, and aggregation of Fe3O4 nanoparticles. Subsequently, negatively enhanced T2‐weighted MRI signal is obtained for visual detection of singlet oxygen in the solution, cancer cells, and in vivo. This oxidation responsive MRI probe is expected to hold great promise in evaluating the ability of photosensitizers to generate singlet oxygen and in predicting the therapeutic efficacies of PDT in vivo.
The relative efficacy of the cranium and os coxa for taxonomic assessment in macaques
Authors: Kenyon‐Flatt Brittany , Conaway Mark A. , Lycett Stephen J. , von Cramon‐Taubadel Noreen 
Published: June 2020

AbstractObjectivesThe cranium is generally considered more reliable than the postcranium for assessing primate taxonomy, although recent research suggests that pelvic shape may be equally reliable. However, little research has focused on intrageneric taxonomic discrimination. Here, we test the relative taxonomic efficacy of the cranium and os coxa for differentiating two macaque species, with and without considering sexual dimorphism.Materials and methodsGeometric morphometric analyses were performed on cranial and os coxa landmarks for 119 adult Macaca fascicularis, M. mulatta, and Chlorocebus pygerythrus. Among‐group shape variation was examined using canonical variates analyses. Cross‐validated discriminant function analysis provided rates of correct group classification. Additionally, average morphological distances were compared with neutral genetic distances.ResultsMacaque species were clearly differentiated, both cranially and pelvically, when sex was not considered. Males were more often correctly classified based on the os coxa, while female classification rates were high for both morphologies. Female crania and male os coxa were differentiated approximately the same as genetic distance, while male crania were more similar (convergent), and female os coxa were more divergent than expected based on genetic distance.DiscussionThe hypothesis that cranial and os coxal shape can be used to discriminate among macaque species was supported. The cranium was better at differentiating females, while the os coxa was better at differentiating male macaques. Hence, there is no a priori reason for preferring the cranium when assessing intragenetic taxonomic relationships, but the effects of high levels of sexual dimorphism must be corrected for to accurately assess taxonomic signatures.
Interfacial Evolution of Lithium Dendrites and Their Solid Electrolyte Interphase Shells of Quasi‐Solid‐State Lithium‐Metal Batteries
Authors: Shi Yang , Wan Jing , Liu Gui‐Xian , Zuo Tong‐Tong , Song Yue‐Xian , Liu Bing , Guo Yu‐Guo , Wen Rui , Wan Li‐Jun 
Published: June 2020

AbstractUnstable electrode/solid‐state electrolyte interfaces and internal lithium dendrite penetration hamper the applications of solid‐state lithium‐metal batteries (SSLMBs), and the underlying mechanisms are not well understood. Herein, in situ optical microscopy provides insights into the lithium plating/stripping processes in a gel polymer electrolyte and reveals its dynamic evolution. Spherical lithium deposits evolve into moss‐like and branch‐shaped lithium dendrites with increasing current densities. Remarkably, the on‐site‐formed solid electrolyte interphase (SEI) shell on the lithium dendrite is distinctly captured after lithium stripping. Inducing an on‐site‐formed SEI shell with an enhanced modulus to wrap the lithium precipitation densely and uniformly can regulate dendrite‐free behaviors. An in‐depth understanding of lithium dendrite evolution and its functional SEI shell will aid in the optimization of SSLMBs.
Light Dynamics of the Retinal‐Disease‐Relevant G90D Bovine Rhodopsin Mutant
Authors: Kubatova Nina , Mao Jiafei , Eckert Carl Elias , Saxena Krishna , Gande Santosh L. , Wachtveitl Josef , Glaubitz Clemens , Schwalbe Harald 
Published: June 2020

AbstractThe RHO gene encodes the G‐protein‐coupled receptor (GPCR) rhodopsin. Numerous mutations associated with impaired visual cycle have been reported; the G90D mutation leads to a constitutively active mutant form of rhodopsin that causes CSNB disease. We report on the structural investigation of the retinal configuration and conformation in the binding pocket in the dark and light‐activated state by solution and MAS‐NMR spectroscopy. We found two long‐lived dark states for the G90D mutant with the 11‐cis retinal bound as Schiff base in both populations. The second minor population in the dark state is attributed to a slight shift in conformation of the covalently bound 11‐cis retinal caused by the mutation‐induced distortion on the salt bridge formation in the binding pocket. Time‐resolved UV/Vis spectroscopy was used to monitor the functional dynamics of the G90D mutant rhodopsin for all relevant time scales of the photocycle. The G90D mutant retains its conformational heterogeneity during the photocycle.
Tighter Confinement Increases Selectivity of d‐Glucose Isomerization Toward l‐Sorbose in Titanium Zeolites
Authors: Cordon Michael J. , Vega‐Vila Juan Carlos , Casper Alyssa , Huang Zige , Gounder Rajamani 
Published: June 2020

AbstractAqueous‐phase isomerization of d‐glucose to d‐fructose and l‐sorbose is catalyzed in parallel by Lewis acidic Ti sites in siliceous frameworks. Glucose isomerization rates (per Ti, 373 K) are undetectable when Ti sites are confined within mesoporous voids (Ti‐MCM‐41, TiO2‐SiO2) and increase to detectable values when Ti sites are confined within the smaller 12‐membered ring (12‐MR) micropores of Ti‐Beta. Isomerization rates decrease to lower values (by ≈20×) with further decreases in micropore size as Ti sites are confined within 10‐MR pores (Ti‐MFI, Ti‐CON), likely because of intrapore reactant diffusion restrictions, and reach undetectable values within the 8‐MR pores of Ti‐CHA as size exclusion prevents glucose from accessing active sites. Remarkably, the selectivity toward l‐sorbose over d‐fructose increases systematically as spatial constraints around Ti sites become tighter, and is >10 on Ti‐MFI. These findings demonstrate the marked influence of confinement around Ti active sites on the selectivity between parallel stereoselective sugar isomerization pathways.
Synthesis and Structure of Functionalized Zigzag Hydrocarbon Belts
Authors: Zhang Yang , Tong Shuo , Wang Mei‐Xiang 
Published: June 2020

AbstractDescribed in this paper are the synthesis and structure of novel and edge‐functionalized zigzag hydrocarbon belts. A stepwise “fjord‐stitching” strategy featuring repetitive intramolecular acylation reactions of a resorcin[4]arene derivative as the key steps afforded a biscarbonyl‐functionalized octahydrobelt[8]arene product. Facile ketone reduction with NaBH4 and nucleophilic addition with n‐butyllithium produced secondary and tertiary alcohol‐containing molecular belts, respectively. Selective oxidation reactions of biscarbonyl‐bearing octahydrobelt[8]arene with m‐CPBA and (PhSeO)2O furnished the corresponding lactone‐ and 1,4‐quinone‐embedded molecular belts. Depending on the functional groups on the edges, the acquired belt molecules adopt different shapes such as square prism, truncated cone, and elliptical cylinder.
Synthesis of a Gold–Metal Oxide Core–Satellite Nanostructure for In Situ SERS Study of CuO‐Catalyzed Photooxidation
Authors: Zhang Kaifu , Yang Ling , Hu Yanfang , Fan Chenghao , Zhao Yaran , Bai Lu , Li Yonglong , Shi Faxing , Liu Jun , Xie Wei 
Published: June 2020

AbstractThis work reports on an assembling–calcining method for preparing gold–metal oxide core–satellite nanostructures, which enable surface‐enhanced Raman spectroscopic detection of chemical reactions on metal oxide nanoparticles. By using the nanostructure, we study the photooxidation of Si−H catalyzed by CuO nanoparticles. As evidenced by the in situ spectroscopic results, oxygen vacancies of CuO are found to be very active sites for oxygen activation, and hydroxide radicals (*OH) adsorbed at the catalytic sites are likely to be the reactive intermediates that trigger the conversion from silanes into the corresponding silanols. According to our finding, oxygen vacancy‐rich CuO catalysts are confirmed to be of both high activity and selectivity in photooxidation of various silanes.
Micro‐Lensed Fiber Laser Desorption Mass Spectrometry Imaging Reveals Subcellular Distribution of Drugs within Single Cells
Authors: Meng Yifan , Cheng Xiaoling , Wang Tongtong , Hang Wei , Li Xiaoping , Nie Wan , Liu Rong , Lin Zheng , Hang Le , Yin Zhibin , Zhang Baolin , Yan Xiaomei 
Published: June 2020

AbstractThe visualization of temporal and spatial changes in the intracellular environment has great significance for chemistry and bioscience research. Mass spectrometry imaging (MSI) plays an important role because of its unique advantages, such as being label‐free and high throughput, yet it is a challenge for laser‐based techniques due to limited lateral resolution. Here, we develop a simple, reliable, and economic nanoscale MSI approach by introducing desorption laser with a micro‐lensed fiber. Using this integrated platform, we achieved 300 nm resolution MSI and successfully visualized the distribution of various small‐molecule drugs in subcellular locations. Exhaustive dynamic processes of anticancer drugs, including releasing from nanoparticle carriers entering nucleus of cells, can be readily acquired on an organelle scale. Considering the simplicity and universality of this nanoscale desorption device, it could be easily adapted to most of laser‐based mass spectrometry applications.
Ultrafast Encapsulation of Metal Nanoclusters into MFI Zeolite in the Course of Its Crystallization: Catalytic Application for Propane Dehydrogenation
Authors: Zhu Jie , Osuga Ryota , Ishikawa Ryo , Shibata Naoya , Ikuhara Yuichi , Kondo Junko N. , Ogura Masaru , Yu Jihong , Wakihara Toru , Liu Zhendong , Okubo Tatsuya 
Published: June 2020

AbstractEncapsulating metal nanoclusters into zeolites combines the superior catalytic activity of the nanoclusters with high stability and unique shape selectivity of the crystalline microporous materials. The preparation of such bifunctional catalysts, however, is often restricted by the mismatching in time scale between the fast formation of nanoclusters and the slow crystallization of zeolites. We herein demonstrate a novel strategy to overcome the mismatching issue, in which the crystallization of zeolites is expedited so as to synchronize it with the rapid formation of nanoclusters. The concept was demonstrated by confining Pt and Sn nanoclusters into a ZSM‐5 (MFI) zeolite in the course of its crystallization, leading to an ultrafast, in situ encapsulation within just 5 min. The Pt/Sn‐ZSM‐5 exhibited exceptional activity and selectivity with stability in the dehydrogenation of propane to propene. This method of ultrafast encapsulation opens up a new avenue for designing and synthesizing composite zeolitic materials with structural and compositional complexity.
Structure and stability analyses of collagen hydrolysate coacervates triggered by ethanol in aqueous solution
Authors: Li Zhijie , Liu Jie , Zhang Luan , Xiao Yuelong , Tang Keyong , Zheng Xuejing , Pei Ying 
Published: June 2020

AbstractThe coacervation of collagen hydrolysate (CH) in aqueous solution was triggered by ethanol as poor solvent which has a significant effect on the aggregation and rearrangement of CH macromolecules. In this work, the static coacervation was performed to investigate the influences on coacervates morphology. CH concentration and ethanol temperature had significant influences on coacervates morphology. Treatment for the CH solution of high concentration by lower temperature ethanol (<25°C) tended to form the microspheres morphology. High temperature ethanol was not conducive to CH coacervation, while enhanced the CH solubilization in water‐ethanol. By directly mixing CH solution and ethanol under agitation, dispersed nanospheres could be successfully obtained in suspension. The turbidity and absorbance tests were carried out to further study the CH coacervation behaviors. The stability of milky suspension induced by ethanol was evaluated by a self‐built light transmittance measurement. The result showed that the absorbance and turbidity of CH suspension increased with increasing the VEtOH ratio and CH concentration. The milky suspension with high VEtOH would turn clear gradually due to the CH solubilization in water–alcohol. The maximum absorbance presented at pH around 7 where the charges of CH molecules were negative. The coacervation behaviors of CH were very different from that of collagen and gelatin, owing to its lower molecular weight.