bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Network Approach for Delineating Homogeneous Regions in Regional Flood Frequency Analysis
Authors: Han Xudong , Ouarda Taha B. M. J. , Rahman Ataur , Haddad Khaled , Mehrotra Rajeshwar , Sharma Ashish 
Published: February 2020

AbstractRegional flood frequency analysis forms the basis for ascertaining design thresholds for extreme flow events for the purpose of resource management and design of hydraulic structures, especially at ungauged or partially gauged basins. A crucial step in this analysis is transferring available information from gauged sites to ungauged sites, which is achieved through delineation of homogeneous regions encompassing multiple catchment locations, followed by the formulation of a flood estimation model. While this process has been accomplished through a range of statistical homogenization alternatives, the present study offers a new approach rooted in the theory of complex networks, offering considerable advantages over what is traditionally followed. Data from 202 sites in Australia representing catchments of varying geographic, climatic, and vegetation attributes are used to assess the alternative proposed. The results are examined via (1) direct comparison of the location and number of homogeneous neighbors from network theory with results using canonical correlation analysis (CCA) and (2) assessing the accuracy of estimated flood quantiles by applying a common model that estimates flood quantiles using information from the two alternate groups of homogeneous sites (from network theory and CCA). Results show that network theory offers merit in delineating homogenous regions, with resulting design flood estimates showing improvements across different return periods compared to the CCA alternative used.
[11C]PBR28 MR–PET imaging reveals lower regional brain expression of translocator protein (TSPO) in young adult males with autism spectrum disorder
Authors: Zürcher N. R. , Loggia M. L. , Mullett J. E. , Tseng C. , Bhanot A. , Richey L. , Hightower B. G. , Wu C. , Parmar A. J. , Butterfield R. I. , Dubois J. M. , Chonde D. B. , Izquierdo-Garcia D. , Wey H. Y. , Catana C. , Hadjikhani N. , McDougle C. J. , Hooker J. M. 
Published: February 2020

AbstractMechanisms of neuroimmune and mitochondrial dysfunction have been repeatedly implicated in autism spectrum disorder (ASD). To examine these mechanisms in ASD individuals, we measured the in vivo expression of the 18 kDa translocator protein (TSPO), an activated glial marker expressed on mitochondrial membranes. Participants underwent scanning on a simultaneous magnetic resonance–positron emission tomography (MR–PET) scanner with the second-generation TSPO radiotracer [11C]PBR28. By comparing TSPO in 15 young adult males with ASD with 18 age- and sex-matched controls, we showed that individuals with ASD exhibited lower regional TSPO expression in several brain regions, including the bilateral insular cortex, bilateral precuneus/posterior cingulate cortex, and bilateral temporal, angular, and supramarginal gyri, which have previously been implicated in autism in functional MR imaging studies. No brain region exhibited higher regional TSPO expression in the ASD group compared with the control group. A subset of participants underwent a second MR–PET scan after a median interscan interval of 3.6 months, and we determined that TSPO expression over this period of time was stable and replicable. Furthermore, voxelwise analysis confirmed lower regional TSPO expression in ASD at this later time point. Lower TSPO expression in ASD could reflect abnormalities in neuroimmune processes or mitochondrial dysfunction.
REEP5 depletion causes sarco-endoplasmic reticulum vacuolization and cardiac functional defects
Authors: Lee Shin-Haw , Hadipour-Lakmehsari Sina , Murthy Harsha R. , Gibb Natalie , Miyake Tetsuaki , Teng Allen C. T. , Cosme Jake , Yu Jessica C. , Moon Mark , Lim SangHyun , Wong Victoria , Liu Peter , Billia Filio , Fernandez-Gonzalez Rodrigo , Stagljar Igor , Sharma Parveen , Kislinger Thomas , Scott Ian C. , Gramolini Anthony O. 
Published: February 2020

AbstractThe sarco-endoplasmic reticulum (SR/ER) plays an important role in the development and progression of many heart diseases. However, many aspects of its structural organization remain largely unknown, particularly in cells with a highly differentiated SR/ER network. Here, we report a cardiac enriched, SR/ER membrane protein, REEP5 that is centrally involved in regulating SR/ER organization and cellular stress responses in cardiac myocytes. In vitro REEP5 depletion in mouse cardiac myocytes results in SR/ER membrane destabilization and luminal vacuolization along with decreased myocyte contractility and disrupted Ca2+ cycling. Further, in vivo CRISPR/Cas9-mediated REEP5 loss-of-function zebrafish mutants show sensitized cardiac dysfunction upon short-term verapamil treatment. Additionally, in vivo adeno-associated viral (AAV9)-induced REEP5 depletion in the mouse demonstrates cardiac dysfunction. These results demonstrate the critical role of REEP5 in SR/ER organization and function as well as normal heart function and development.
Immune checkpoint modulation enhances HIV-1 antibody induction
Authors: Bradley Todd , Kuraoka Masayuki , Yeh Chen-Hao , Tian Ming , Chen Huan , Cain Derek W. , Chen Xuejun , Cheng Cheng , Ellebedy Ali H. , Parks Robert , Barr Maggie , Sutherland Laura L. , Scearce Richard M. , Bowman Cindy M. , Bouton-Verville Hilary , Santra Sampa , Wiehe Kevin , Lewis Mark G. , Ogbe Ane , Borrow Persephone , Montefiori David , Bonsignori Mattia , Anthony Moody M. , Verkoczy Laurent , Saunders Kevin O. , Ahmed Rafi , Mascola John R. , Kelsoe Garnett , Alt Frederick W. , Haynes Barton F. 
Published: February 2020

AbstractEliciting protective titers of HIV-1 broadly neutralizing antibodies (bnAbs) is a goal of HIV-1 vaccine development, but current vaccine strategies have yet to induce bnAbs in humans. Many bnAbs isolated from HIV-1-infected individuals are encoded by immunoglobulin gene rearrangments with infrequent naive B cell precursors and with unusual genetic features that may be subject to host regulatory control. Here, we administer antibodies targeting immune cell regulatory receptors CTLA-4, PD-1 or OX40 along with HIV envelope (Env) vaccines to rhesus macaques and bnAb immunoglobulin knock-in (KI) mice expressing diverse precursors of CD4 binding site HIV-1 bnAbs. CTLA-4 blockade augments HIV-1 Env antibody responses in macaques, and in a bnAb-precursor mouse model, CTLA-4 blocking or OX40 agonist antibodies increase germinal center B and T follicular helper cells and plasma neutralizing antibodies. Thus, modulation of CTLA-4 or OX40 immune checkpoints during vaccination can promote germinal center activity and enhance HIV-1 Env antibody responses.
Successive redox-mediated visible-light ferrophotovoltaics
Authors: Noguchi Yuji , Taniguchi Yuki , Inoue Ryotaro , Miyayama Masaru 
Published: February 2020

AbstractTitanium oxide materials have multiple functions such as photocatalytic and photovoltaic effects. Ferroelectrics provide access to light energy conversion that delivers above-bandgap voltages arising from spatial inversion symmetry breaking, whereas their wide bandgap leads to poor absorption of visible light. Bandgap narrowing offers a potential solution, but this material modification suppresses spontaneous polarization and, hence, sacrifices photovoltages. Here, we report successive-redox mediated ferrophotovoltaics that exhibit a robust visible-light response. Our single-crystal experiments and ab initio calculations, along with photo-luminescence analysis, demonstrate that divalent Fe2+ and trivalent Fe3+ coexisted in a prototypical ferroelectric barium titanate BaTiO3 introduce donor and acceptor levels, respectively, and that two sequential Fe3+/Fe2+ redox reactions enhance the photogenerated power not only under visible light but also at photon energies greater than the bandgap. Our approach opens a promising route to the visible-light activation of photovoltaics and, potentially, of photocatalysts.
Organocatalytic asymmetric N-sulfonyl amide C-N bond activation to access axially chiral biaryl amino acids
Authors: Wang Guanjie , Shi Qianqian , Hu Wanyao , Chen Tao , Guo Yingying , Hu Zhouli , Gong Minghua , Guo Jingcheng , Wei Donghui , Fu Zhenqian , Huang Wei 
Published: February 2020

AbstractAmides are among the most fundamental functional groups and essential structural units, widely used in chemistry, biochemistry and material science. Amide synthesis and transformations is a topic of continuous interest in organic chemistry. However, direct catalytic asymmetric activation of amide C-N bonds still remains a long-standing challenge due to high stability of amide linkages. Herein, we describe an organocatalytic asymmetric amide C-N bonds cleavage of N-sulfonyl biaryl lactams under mild conditions, developing a general and practical method for atroposelective construction of axially chiral biaryl amino acids. A structurally diverse set of axially chiral biaryl amino acids are obtained in high yields with excellent enantioselectivities. Moreover, a variety of axially chiral unsymmetrical biaryl organocatalysts are efficiently constructed from the resulting axially chiral biaryl amino acids by our present strategy, and show competitive outcomes in asymmetric reactions.
The spatio-temporal features of chicken mitochondrial ND2 gene heteroplasmy and the effects of nutrition factors on this gene
Authors: Yang Suliang , Huo Yangyang , Wang Huanjie , Ji Jiefei , Chen Wen , Huang Yanqun 
Published: February 2020

AbstractMitochondrial heterogeneity is the presence of two or more types of mitochondrial (mt)DNA in the same individual/tissue/cell. It is closely related to animal health and disease. ND2 is a protein-coding gene in mtDNA, which participates in mitochondrial respiratory chain and oxidative phosphorylation. In previous studies, we observed that the mt.A5703T and mt.T5727G sites in the ND2 gene were the heteroplasmic variation sites. We used pyrophosphate sequencing technology to examine chicken mt.A5703T and mt.T5727G heteroplasmic sites in the ND2 gene, in different tissues and at different development stages in chickens. We also investigated whether nutritional factors could affect the mt.A5703T and mt.T5727G heteroplasmy. Our results showed that chicken mt.A5703T and mt.T5727G heteroplasmy had clear spatio-temporal specificities, which varied between tissues/development stages. The mtDNA heterogeneity was relatively stable upon nutrition intervention, 30% dietary energy restriction (from 18 to 48 days old) and different types of dietary fats (at 5% concentration, from 1 to 42 days old) did not change the breast muscle heteroplasmy of broilers at the mt.A5703T and mt.T5727G sites. In addition, multiple potential heteroplasmic sites were detected by clone sequencing in the ND2 region, which potentially reflected abundant heteroplasmy in the chicken mitochondrial genome. These results provide an important reference for further research on heteroplasmy in chicken mitochondria.
Immunohistochemical selection of biomarkers for tumor-targeted image-guided surgery of myxofibrosarcoma
Authors: de Gooyer Jan Marie , Versleijen-Jonkers Yvonne M. H. , Hillebrandt-Roeffen Melissa H. S. , Frielink Cathelijne , Desar Ingrid M. E. , de Wilt Johannes H. W. , Flucke Uta , Rijpkema Mark 
Published: February 2020

AbstractMyxofibrosarcoma(MFS) is the most common soft tissue sarcoma(STS) in elderly patients. Surgical resection remains the main treatment modality but tumor borders can be difficult to delineate with conventional clinical methods. Incomplete resections are a common problem and local recurrence remains a clinical issue. A technique that has shown great potential in improving surgical treatment of solid tumors is tumor targeted imaging and image-guided surgery with near-infrared fluorescence. To facilitate this technique, it is essential to identify a biomarker that is highly and homogenously expressed on tumor cells, while being absent on healthy non-malignant tissue. The purpose of this study was to identify suitable molecular targets for tumor-targeted imaging of myxofibrosarcoma. Ten potential molecular targets for tumor targeted imaging were investigated with immunohistochemical analysis in myxofibrosarcoma tissue (n = 34). Results were quantified according to the immunoreactive score(IRS). Moderate expression rates were found for uPAR, PDGFRa and EMA/MUC1. High expression rates of VEGF and TEM1 were seen. Strong expression was most common for TEM1 (88.2%). These results confirms that TEM1 is a suitable target for tumor-targeted imaging of myxofibrosarcoma. Keywords Image-guided surgery; Immunohistochemistry; Molecular imaging; Myxofibrosarcoma; Soft tissue sarcoma; Tumor endothelial marker 1(TEM1), Vascular endothelial growth factor (VEGF).
Lead palladium titanate: A room temperature nanoscale multiferroic thin film
Authors: Mishra K. K. , Instan Alvaro A. , Kumari Shalini , Scott J. F. , Katiyar Ram S. 
Published: February 2020

AbstractThe discovery of single-phase multiferroic materials and the understanding of coupling mechanisms between their spin and polarization is important from the point of view of next generation logic and memory devices. Herein we report the fabrication, dielectric, ferroelectric, piezo-response force microscopy, and magnetization measurements of Pd-substituted room-temperature magnetoelectric multiferroic PbPd0.3Ti0.7O3 (PbPdT) thin films. Highly oriented PbPdT thin films were deposited on {(LaAlO3)0.3(Sr2AlTaO6)0.7} (LSAT) substrates in oxygen atmosphere using pulsed laser deposition technique. X-ray diffraction studies revealed that the films had tetragonal phase with (001) orientation. Surface morphology studies using atomic force and scanning electron microscopy suggest a smooth and homogeneous distribution of grains on the film surface with roughness ~2 nm. A large dielectric constant of ~1700 and a low-loss tangent value of ~0.3 at 10 kHz were obtained at room temperature. Temperature dependent dielectric measurements carried out on Pt/PbPdT/La0.7Sr0.3MnO3 (LSMO) metal-dielectric-metal capacitors suggest a ferroelectric to paraelectric transition above 670 K. The measured polarization hysteresis loops at room temperature were attributed to its ferroelectric behavior. From a Tauc plot of (αhν)2 versus energy, the direct band gap Eg of PbPdT thin films was calculated as 3 eV. Ferroelectric piezoelectric nature of the films was confirmed from a strong domain switching response revealed from piezo-response force microscopy. A well-saturated magnetization M-H loop with remanent magnetization of 3.5 emu/cm3 was observed at room temperature, and it retains ferromagnetic ordering in the temperature range 5–395 K. Origin of the magnetization could be traced to the mixed oxidation states of Pd2+/Pd4+ dispersed in polar PbTiO3 matrix, as revealed by our x-ray photoelectron spectroscopic results. These results suggest that PbPdT thin films are multiferroic (ferroelectric-ferromagnetic) at room temperature.
Psychokardiologie bei koronaren Herzerkrankungen
Authors: Peregrinova Ludmila , Hamann Bettina 
Published: February 2020

ZusammenfassungDie Psychokardiologie befasst sich mit den bidirektionalen Zusammenhängen zwischen Herzerkrankungen und psychischen Störungen. Privater und beruflicher Stress, psychische Erkrankungen sowie Persönlichkeitseigenschaften können das gesundheitliche Risikoverhalten begünstigen und folglich den Verlauf von kardiovaskulären Erkrankungen negativ beeinflussen. Hingegen lösen akute kardiale Ereignisse und chronische Herzerkrankungen häufig eine behandlungsbedürftige psychische Symptomatik aus, die die Krankheitsbewältigung beeinträchtigt und die Mortalität erhöht. Die ungünstigen psychosozialen Risikokonstellationen sollten somit identifiziert und behandelt werden, um langfristig positives Gesundheitsverhalten sowie die Lebensqualität der Patienten zu fördern und aufrechtzuerhalten.
Ozontherapie bei der Koronaren Herzerkrankung
Authors: Dorstewitz Hartmut 
Published: February 2020

ZusammenfassungDie Ozon-Sauerstoff-Therapie kann in der Therapie der Koronaren Herzerkrankung im Rahmen eines komplementärmedizinischen Behandlungskonzepts einen wertvollen Beitrag leisten. Studien zur KHK mit Ozontherapie fehlen bislang, kleinere Studien u. a. zur peripheren arteriellen Verschlusskrankheit konnten positive Effekte untermauern. Die Ozontherapie ist deswegen bei KHK indiziert, weil diese Erkrankung, ganz ähnlich wie die periphere arterielle Verschlusskrankheit, ein vaskuläres Problem darstellt. Dabei spielt die Endothelfunktion eine entscheidende Rolle. Der antiinflammatorische Effekt durch Ozon-Sauerstoff-Therapie reduziert deutlich den oxidativen Stress und vermindert so die Endothelschädigung.Die Ozontherapie erfolgt in der Praxis in Form der Großen Eigenbluttherapie oder seltener als Rektalinsufflation. Ein Fall aus der Praxis zeigt beispielhaft das Vorgehen und die möglichen Behandlungserfolge.
Introduction
Authors: Giaxoglou Korina , Johansson Marjut 
Published: February 2020

Abstract
This introduction to the Special Issue on Networked Emotion and Stancetaking summarizes the individual and
collective contribution of the included five research articles. We argue for the relevance of discourse-pragmatic theories,
methods, and concepts for furnishing cross-disciplinary perspectives into the study of emotion online. Such perspectives are
arguably needed in order to clarify the intricate connections between (re)presentations of emotion online and changing practices
of news-making and news consumption, story sharing and participation, and public stancetaking in social media and beyond. We
propose that empirical analyses of networked practices of stancetaking – epistemic, affective, or narrative – can
pinpoint the construction and dissemination of different types of participant positions and stances, including multimodal ones, as
well as the creation and uptake of specific frames for interpreting events and crises affectively.
Development of aluminum paste with/without boron content for crystalline silicon solar cells
Authors: Uzum Abdullah , Ashikaga Taiki , Noguchi Takuma , Kanda Hiroyuki , Fukui Hidehito , Harada Tomitaro , Ito Seigo 
Published: February 2020

Abstract
Improvement of aluminum alloyed p + back surface fields (p + BSF) which is an essential requirement for achieving high efficiency silicon solar cells has been an important task. One of the ways to have better quality BSFs can be to introduce screen printable aluminum pastes with boron content. Two type of pastes were developed in this work and recipes were provided in detail: screen-printable aluminum paste without boron content (B-free-Al-paste), screen-printable aluminum paste with boron content (Al-B-paste). The ingredients of the pastes were optimized and basically evaluated in terms of alloying and impurity characteristics by measurement of sheet resistances, carrier lifetimes and SIMS analysis. Carrier lifetimes of the wafers processed by Al-B-paste maintained at around 300 μs relatively higher than the wafers processed by B-free-Al-paste. P-type silicon solar cells were fabricated using developed pastes and were compared with those of the cells fabricated by commercial aluminum pastes. Best efficiency of 17.8% was achieved with totally vacuum-less cell production process and Suns-Voc analysis were also carried out for fabricated solar cells.
S100A4 promotes the progression of lipopolysaccharide-induced acute epididymitis in mice†
Authors: Wu Yingjie , Li Haoran , Qin Yinghe 
Published: February 2020

Abstract
S100A4 has been suggested to be a critical regulator of tumor metastasis and is implicated in the progression of inflammation. The aim of this study is to investigate the expression and possible role of S100A4 in epididymitis. Using a mouse model of epididymitis induced by the injection of lipopolysaccharide (LPS) in the deferent duct, we found that LPS administration induced an upregulation of S100a4 transcription (P &lt; 0.05) and a recruitment of S100A4 positive cells in the epididymal interstitium of wild type (WT) mice. Co-immunofluorescence showed that S100A4 was mainly expressed by granulocytes, CD4 lymphocytes, and macrophages. Deficiency of S100A4 reduced epididymal pathological reaction and the mRNA levels of the pro-inflammatory cytokines IL-1β and TNF-α (P &lt; 0.01), suggesting that S100A4 promotes the progression of epididymitis. Furthermore, S100A4 deficiency alleviated the decline of sperm motility and rectified the abnormal expression of sperm membrane protein AMAD3, which suggested that in the progression of epididymitis, S100A4 aggravates the damage to sperm vitality. In addition, both Ki-67 marked cell proliferation and transferase-mediated dUTP-biotin nick end labeling detected cell apoptosis were reduced in S100a4−/− mice compared with WT mice after LPS treatment, indicating that S100A4 promotes both cell proliferation and cell apoptosis in epididymitis. Overall, these results demonstrate that S100A4 promotes the progression of LPS-induced epididymitis and facilitates a decline in sperm vitality, and its function may be related to the process of cell proliferation and apoptosis during inflammation.
Genetic Analysis of Ulcerative Colitis in Japanese Individuals Using Population-specific SNP Array
Authors: Okamoto Daisuke , Kawai Yosuke , Kakuta Yoichi , Naito Takeo , Torisu Takehiro , Hirano Atsushi , Umeno Junji , Fuyuno Yuta , Li Dalin , Nakano Takeru , Izumiyama Yasuhiro , Ichikawa Ryo , Hiramoto Keiichiro , Moroi Rintaro , Kuroha Masatake , Kanazawa Yoshitake , Shiga Hisashi , Tokunaga Katsushi , Nakamura Minoru , Esaki Motohiro , Matsumoto Takayuki , McGovern Dermot P B , Nagasaki Masao , Kinouchi Yoshitaka , Masamune Atsushi 
Published: February 2020

Abstract

Background
To clarify the genetic background of ulcerative colitis (UC) in the Japanese population, we conducted a genome-wide association study (GWAS) using a population-specific single nucleotide polymorphism (SNP) array.

Methods
We performed a GWAS and replication study including 1676 UC patients and 2381 healthy controls. The probability of colectomy was compared between genotypes of rs117506082, the top hit SNP at HLA loci, by the Kaplan-Meier method. We studied serum expression of miR-622, a newly identified candidate gene, from 32 UC patients and 8 healthy controls by quantitative reverse-transcription polymerase chain reaction.

Results
In the GWAS, only the HLA loci showed genome-wide significant associations with UC (rs117506082, P = 6.69E-28). Seven nominally significant regions included 2 known loci, IL23R (rs76418789, P = 6.29E-7) and IRF8 (rs16940202, P = 1.03E-6), and 5 novel loci: MIR622 (rs9560575, P = 8.23E-7), 14q31 (rs117618617, P = 1.53E-6), KAT6B (rs12260609, P = 1.81E-6), PAX3-CCDC140-SGPP2 (rs7589797, P = 2.87E-6), and KCNA2 (rs118020656, P = 4.01E-6). Combined analysis revealed that IL23R p.G149R (rs76418789, P = 9.03E-11; odds ratio [OR], 0.51) had genome-wide significant association with UC. Patients with GG genotype of rs117506082 had a significantly lower probability of total colectomy than those with the GA+AA genotype (P = 1.72E-2). Serum expression of miR-622 in patients with inactive UC tended to be higher than in healthy controls and patients with active UC (inactive UC vs healthy controls, P = 3.03E-02; inactive UC vs active UC, P = 6.44E-02).

Conclusions
IL23R p.G149R is a susceptibility locus for UC in Japanese individuals. The GG genotype of rs117506082 at HLA loci may predict a better clinical course.