bluuebunny/crossref_metadata_2025_split huggingface dataset:
Semi Field Evaluation of Freestanding Transfluthrin Passive Emanators and the BG Sentinel Trap as a “Push-Pull control Strategy” Against Aedes aegypti Mosquitoes
Authors: Tambwe Mgeni Mohamed , Moore Sarah , Chilumba Hassan , Swai Johnson Kyeba , Moore Jason , Stica Caleb , Saddler Adam 
Published: May 2020

Abstract
Background The use of volatile pyrethroids and odor-baited traps in a push-pull system has been shown to reduce house entry and outdoor bites for malaria vectors. This technology has the potential to control other outdoor biting mosquitoes such as Aedes aegypti that transmit arboviral diseases. In this study, semi-field experiments were conducted to evaluate whether a push-pull system could be used to reduce bites from Aedes.Methods The push and pull under investigation consisted of two freestanding transfluthrin passive emanators (FTPE), and a BG sentinel trap (BGS) respectively. The FTPE contained hessian strips treated with 5.25 g of transfluthrin active ingredient. The efficacies of FTPE and BGS alone and in combination were evaluated by human landing catch in a large semi-field system in Tanzania. We also investigated the protection of FTPE over six months. The data was analyzed using generalized linear mixed models with binomial distribution.Results Two FTPE have a protective efficacy (PE) of 61% (95% Confidence interval (CI): 52.18–69.91) against human landing rate of Aedes aegypti. The BGS did not significantly reduce mosquito landings; the PE was 2.14% (95% CI: -2.87-7.16). The combination of FTPE and BGS (push-pull) provided the PE of 64.46% (95% CI: 59.06–69.85). However, there was no significant difference in the protective efficacy between the push-pull and the two FTPE against Ae. aegypti (p = 0.286). The FTPE offered significant protection against Ae. aegypti at month three, with a PE of 46.44% (95% CI: 41.12–51.76), but not at six months with a PE of 2.20% (95% CI: -9.00-14.02).Conclusions The protective efficacy of the FTPE and the full push-pull system are similar, indicative that bite prevention is primarily due to the activity of the FTPE. While these results are encouraging for the FTPE, further work is needed for a push-pull system to be recommended for Ae. aegypti control. The three-month protection provided by the FTPE against Ae. aegypti bites suggests it would be a useful additional personal protection tool during dengue outbreaks, that does not require regular user compliance.
Development of an Efficient and Stable Transformation System for Aspergillus Oryzae Based on the pyrG Gene
Authors: Zhou Caixia , Wan Yujun , Yao Huipeng , Chen Hui , Xiao Yirong , Tang Zizhong , Shan Zhi , Bu Tongliang , Chen Hong , Wang Gang 
Published: May 2020

Abstract
Background Aspergillus oryzae is an ideal host for expressing heterologous and homologous genes. An efficient and stable transformation system is the key to the successful expression of the gene of interest in A. oryzae.Results To improve the expression efficiency of the gene of interest in A. oryzae, we constructed the uridine/uracil auxotrophic strains A. oryzae RIB40ΔpyrG by Ultraviolet (UV) mutagenesis of pyrG gene deletion which would be used as a host for further transformation. In addition, a novel and efficient expression vector pBC-hygro.4 was constructed, including the pyrG cassette gene, His-Tag, amyB promoter and terminator,and green fluorescent protein GFP marker. pBC-hygro.4 transformed A. oryzae RIB40ΔpyrG efficiently via the PEG-CaCl2-mediated transformation method, and the stability of pBC-hygro.4 was tested by detecting the expression of the GFP reporter gene. Through phenotyping and sequencing verification, we successfully obtained a uridine/uracil auxotrophic strains A. oryzae RIB40ΔpyrG. At the same time, the developed vectors are fully functional for heterologous expression of the GFP fluorescent proteins in the A. oryzae RIB40ΔpyrG.Conclusion Our work provides a new method that can be applied to other filamentous fungi to develop similar fungal transformation systems based on auxotrophic/nutritional markers for food-grade recombination applications.
The emerging COVID-19 research: dynamic and regularly updated science maps and analyses
Authors: Andersen Njål , Bramness Jørgen Gustav , Lund Ingunn Olea 
Published: May 2020

Abstract
Background: The COVID-19 pandemic is a global public health emergency and experts emphasize the need for rapid and a high degree of communication and interaction between all parties, in order for critical research to be implemented. We introduce a resource (website) that provides bibliometric analysis showing the current content and structure of the published literature. As new research is published daily, the analysis is regularly updated to show the status as the research field develops and matures. Methods: Two bibliometric methods were employed, the first is a key-word co-occurrence analysis, based on published work available from PubMed. The second is a bibliometric coupling analysis, based on articles available through Scopus. The results are presented as clustered network graphs; available as interactive network graphs through the webpage. Results: For research as of March 23rd, keyword co-occurrence analysis showed that research was organized in 4 topic clusters: “Health and pandemic management”, “The disease and its pathophysiology”, “Clinical epidemiology of the disease” and “Treatment of the disease”. Coupling analyses resulted in 4 clusters on literature that relates to “Overview of the new virus”, “Clinical medicine”, “On the virus” and “Reproduction rate and spread”. Conclusions: We introduced a dynamic resource that will give a wide readership an overview of how the structure of the COVID-19 literature is developing. To illustrate what this can look like, we showed the structure as it stands three months after the virus was identified; the structure is likely to change as we progress to later stages of this pandemic.
Domestic Violence in Mozambique: From policy to practice
Authors: Jethá Eunice , Keygnaert Ines , Martins Emilia , Sidat Mohsin , Roelens Kristien 
Published: May 2020

Abstract
Background: To reduce the impact of domestic violence (DV), Mozambican governmental and non-governmental entities are making efforts to strengthen the legislative framework and improve the accessibility of care services for survivors of violence. Despite this remarkable commitment, the translation of policies and legislation into actions remains a huge challenge. Therefore, this paper aims to identify gaps in the implementation of existing national policies and laws for DV in the services providing care for survivors of DV. Methods: This study, using a qualitative approach had two components. The first was content analysis of guidelines and protocols for DV care provision. The second consisted of in-depth interviews with institutional gender focal points. The analysis of the document content was based on a framework developed according to key elements recommended by international agencies (PAHO and UN) for DV policies and strategies design. Data from the in-depth interviews, where analysed in accordance with study objectives.Results: Eleven (11) guidelines/protocols of care provision and innumerable brochures and pamphlets were identified and analysed. There is a standardised form which highlights the integrated approach incorporating fields for police and the health sector but excludes Civil Society Organizations. However, there is no national DV database. Although the focal points recognised the relevance of the reviewed documents, many identified gaps in implementation. This is related to the weaknesses of the offender’s penalisation and to the scarcity of care providers. And, many providers who do exist often lack appropriate training. They also recognised their performance is negatively influenced by socio-cultural factors.Conclusion: Within services providing care to survivors of DV, a scarcity of guidelines and protocols exist, compromising the quality and standardisation of care. Recognition of the relevance of care guidelines and protocols is a strength, there are challenges governmental and non-governmental entities must address in order to ensure DV prevention and control strategies have the desired results.
The Magnitude of Suicidal Behaviour among People Living with Diabetes Mellitus Attending an Outpatient Department of Alamata General Hospital, Mekelle, Tigray, Ethiopia 2019: a Cross-Sectional Study
Authors: Gebreselassie Abreha Tsegay , Metekiya Workua Mekonen , Beyene Birhane Gebrehiwot 
Published: May 2020

Abstract
Background

Globally, suicide accounts 75% in low and middle-income countries (LMICs).Though the magnitude of suicidal behaviour in high- income countries (HIC) is higher relative to the general population, limited studies had explored about suicidal behaviour among medical outpatients in middle-income countries (LMICs) including this locality. Suicidal behaviours among people with the chronic medical illness are one of the commonest psychiatric emergencies that demand a major health concern by researchers and mental health task forces. People with the chronic medical illness show suicidal ideation and attempt which are fatal problems to end life. Therefore, this study will address the gaps by determining the magnitude of suicidal behaviour among Diabetes Mellitus patients in an outpatient setting of Alamata General Hospital (AGH).
Methods

Institution based cross-sectional study was conducted among medical patients attending a chronic care clinic in Alamata general Hospital from May to June 2019. A sample of 146 DM patients who were attending an outpatient chronic care clinic was included in the study. Patients’ data on socio-demographics, clinical factors, substance use and other information's were collected using a pre-tested structured questionnaire through face to face interview. Suicidal behaviour was used assessed by WHO suicidal behaviour assessment. The collected data were coded, entered and analyzed using frequency, cross-tabulation analysis by SPSS Version 23.
Result

The magnitude of suicidal behaviour among DM patients at AGH is 30.8%.
Conclusion

This study demonstrated that suicidal behaviours common problem in diabetic patients was high magnitude.
The role of Interleukin-33 in patients with mild cognitive impairment and Alzheimer’s disease
Authors: Liang Chih-Sung , Su Kuan-Pin , Tsai Chia-Lin , Lee Jiunn-Tay , Chu Che-Sheng , Yeh Ta-Chuan , Su Ming-Wei , Lin Guan-Yu , Lin Yu-Kai , Chu Hsuan-Te , Tsai Chia-Kuang , Yang Fu-Chi 
Published: May 2020

Abstract
Background: The neuroprotective role of Interleukin (IL)-33 is supported in numerous pre-clinical studies but remains mostly uninvestigated in clinical studies of Alzheimer’s disease (AD). We aimed to examine the association between human blood levels of IL-33 and cognitive preservation in amnestic mild cognitive impairment (aMCI) and AD.Methods: A total of 100 participants (26 controls, 35 aMCI patients, and 39 AD patients) were completed twice Mini Mental State Examination (MMSE) over a 1-year interval. At the second MMSE, the 100 participants examined the plasma levels of IL-33, IL-β, IL-1 receptor agonist (IL-1RA), beta amyloid (Aβ), and tau and apolipoprotein E (ApoE) genotyping, and Hopkins Verbal Learning Test, Trail Making Test, forward and backward digit span, and Clinical Dementia Rating were performed as well. Results: IL-33 expression showed a positive trend among controls (1/26 = 3.8%), aMCI (9/35 = 25.7%), and AD (17/39 = 43.6%) (trend analysis: P < 0.001). The patients expressing IL-33 preserved their cognitive function compared with IL-33 non-expressing patients (1-year ΔMMSE: 0.16 ± 1.6 vs -1.5 ± 2.6; P = 0.006). The cognitive preservation was not associated with the lower levels of Aβ, tau, and AopE ε4, while higher levels of AopE ε4 and phosphorylated tau were indeed associated with cognitive decline. The aMCI patients with AD conversion during study period had higher proportion of IL-33(-) than non-AD converters (0.9% vs 53.3%, P = 0.04).Conclusions: IL-33 or its associated signaling pathways may represent a new treatment paradigm for aMCI and AD.
Respiratory Viral Co-infection with Novel Coronavirus in Children: A Case Series
Authors: Hertzberg Emily , Lim C. Anthoney , Eiting Erick , Yung Steven , Nunez Jeranil , Calderon Yvette , Barnett Barbara 
Published: May 2020

Abstract
Background:The novel coronavirus, COVID-19 was identified in January 2020 initially in Wuhan, China but quickly spread worldwide and was declared a global pandemic by the World Health Organization on March 11, 2020. There are more than 185 countries impacted and the numbers of cases and deaths continues to rise. The rate of coinfections with COVID-19 in children and the clinical implications are unknown.Case Presentation:We describe the clinical presentation and course of three patients with COVID-19 and coinfections with other common respiratory viruses. Two cases were diagnosed with COVID-19 as well as rhinovirus/enterovirus and the third case was COVID-19 and pertussis. Each case had a unique presentations and hospital courses including an emergency room discharge, urgent surgical evaluation and an intensive care unit admission.Conclusions:This is the first US-based case report to discuss coinfections with COVID-19 and other respiratory viruses. Decisions regarding diagnosis and management of children in the setting of the current pandemic should account for the possibility of coinfections. We also highlight publish health challenged resulting from children with the novel coronavirus.
Favorable outcomes of elderly COVID-19 patients in Guangzhou, China: a retrospective, observational study
Authors: Xu Gang , Zhao Jun , Zhang Fuchun , Liu Feng , Feng Congrui , Hu Yudong , Chen Yuluo , Wang Liuqian , Tong Yuwei , Li Yueping , Shi Haiyan , Ma Wei 
Published: May 2020

Abstract
Objective: To clarify the outcomes of elderly patients with COVID-19.Methods: All 265 confirmed adult patients with COVID-19 were included in this retrospective study, 43 (16.2%) of whom were 65 years and older. Electronic medical records of the subjects were reviewed to obtain information on clinical characteristics and outcomes. The allocations of medical resource were also recorded.Results: Only one death case occurred in the elderly. The mortality of elderly patients was no higher than that of young patients (2.3% vs. 0%, P = 0.126). The cure rate was 95.3% in elderly patients and 99.5% in young patients (P = 0.067), and the duration of hospitalization is 27 days in elderly patients and 18 days in young patients (P = 0.001). The elderly suffered from more comorbidities (67.4% vs. 24.8%, P < 0.001), most of which is hypertension. Significantly more severe cases occurred in elderly patients compared with young patients (37.2% vs. 16.7%, P = 0.004). The elderly were more likely to present with complications including acute respiratory distress syndrome, acute myocardial injury, septic shock and acute kidney injury (all P < 0.05), respectively. No medical staffs were infected during the treatment of COVID-19.Conclusion: The cure rate and the mortality of the elderly seemed to be no worse than that of the young, though the elderly were with longer hospitalization. Elderly patients with COVID-19 could be treatable if handled properly. More severe cases and complications in elderly patients should prompt for more complex treatment and special considerations.
Radiological and clinical characteristics of 22 suspected COVID-19 patients with multiple negative RT-PCR testing
Authors: Wu Wenjun , Shi Heshui , Liang Bo , Wu Feihong , Xiang Dongqiao , Qin Ziji , Zhou Hongyan , Zheng Chuansheng , Wang Lixia 
Published: May 2020

Abstract
This study aims to investigate radiological and clinical characteristics of suspected COVID-19 patients with negative RT-PCR testing. 22 suspected COVID-19 patients with 2-5 negative RT-PCR testing were enrolled. The most common symptoms at onset included fever (18[81.8%] patients), fatigue (16[72.7%]) and cough (12[54.5%]). 20(90.9%) patients were with normal or decreased Leukocyte count, 17 (77.3%) with decreased Lymphocyte count, 16 (72.7%) and 18 (81.8%) with increased level of C-reactive protein and D-dimers, respectively. Ground-glass opacity (GGO) (22[100]), reticular/interlobular septal thickening (11 [50%]) and consolidation (14 [63.6%)) with predominantly bilateral and peripheral distribution were typical findings at initial CT scan. The CT score of right lower lobe was significantly higher than right upper lobe (P=0.042). The total CT scores of group 2, 3, 4 (2nd week, 3rd week, 4th week and after) were significantly higher than group 1 (1st week) (P＜0.01,for all). After rapid progression at first week, the most extensive pulmonary involvement emerged at second week after symptom onset, then the involvement gradually decreased. Given the clinical symptoms, laboratory results and typical radiological manifestations, even suspected COVID-19 patients with multiple negative RT-PCR testing deserve great concern for active management.
Rapid and Scalable Production of Functional Anti-Coronavirus Monoclonal Antibody CR3022 in Plants
Authors: Rattanapisit Kaewta , Shanmugaraj Balamurugan , Manopwisedjaroen Suwimon , Purwono Priyo Budi , Siriwattananon Konlavat , Khorattanakulchai Narach , Hanittinan Oranicha , Boonyayothin Wanuttha , Thitithanyanont Arunee , Smith Duncan R. , Phoolcharoen Waranyoo 
Published: May 2020

Abstract
Severe acute respiratory syndrome coronavirus-2 is responsible for an ongoing global outbreak of coronavirus disease (COVID-19) and represents a significant public health threat. The rapid spread of COVID-19 necessitates the development of cost-effective technology platforms for the production of diagnostic reagents/biopharmaceuticals for COVID-19. We explored the possibility of producing an anti-SARS-CoV monoclonal antibody (mAb) CR3022 and the receptor binding domain (RBD) of SARS-CoV-2 in Nicotiana benthamiana. Both RBD and the mAb were transiently expressed with the expression of 8μg/g and 130μg/g leaf fresh weight respectively. The plant-purified mAb binds to SARS-CoV-2, but fails to neutralize it in vitro. This is the first report showing the functional characterization of an anti- SARS-CoV mAb CR3022 in plants. Overall these findings showed that plants are a promising platform to produce anti-SARS-CoV mAb to use as a research reagent or a biotherapeutic in a cost-effective manner, which is especially important to developing economies during epidemics.
On the predictability of COVID-19 in USA: A Google Trends analysis
Authors: Mavragani Amaryllis , Gillas Konstantinos 
Published: May 2020

Abstract
During the difficult times that the world is facing due to the COVID-19 pandemic that has already had severe consequences in all aspects of our lives, it is imperative to explore novel approaches of monitoring and forecasting the regional outbreaks as they happen or even before they do. In this paper, the first approach of exploring the role of Google query data in the predictability of COVID-19 in the US at both national and state level is presented. The results indicate that Google Trends correlate with COVID-19 data, while the estimated models exhibit strong predictability of COVID-19. In line with previous work that has argued on the value of online real-time data in the monitoring and forecasting epidemics and outbreaks, it is evident that such infodemiology approaches can assist public health policy makers, in order to address the most crucial issue; that of flattening the curve, allocating health resources, and increasing the effectiveness and preparedness of the respective health care systems.
A PROTEÇÃO DOS DIREITOS FUNDAMENTAIS PELA AÇÃO FOTOJORNALÍSTICA: O ESTUDO DE CASO JAMES NATCHWEY
Authors: TAVARES Ingrid Helena , ROCHA Manoel Ilson Cordeiro 
Published: May 2020

A indagação do presente artigo científico fundamenta-se na proteção de direitos fundamentais do Direito Internacional Humanitário pela ação artística foto jornalística: o estudo de caso James Natchwey.O assunto central em que a temática se insere encontra-se no rol das regras e princípios fundamentais do Direito Internacional Humanitário. No momento em que se reconhece no acervo fotográfico de Natchwey o grito de socorro da humanidade percebe-se que a conseqüência dos conflitos armados envolve desde o estado de miserabilidade dos povo até a negligência no âmbito da saúde. Tratados internacionais surgiram com o intuito de coibir as práticas penosas e desumanas no contexto da Guerra, porém, para que surgissem tais tratados, a sociedade civil estabeleceu formas denunciativas pleiteando melhores condições para os civis e ex combatentes. Natchwey usou suas fotografias para denunciar o que presenciava nos campos de batalha. Através de seu olhar sensível contou sobre a cultura da tortura, do genocídio e da violência. A cada fotografia exposta demonstrar-se-á a referida violação dos Direitos Fundamentais, bem como a tipificação concernente para coibir tal ato.
Ultrafiltration membranes in managed aquifer recharge systems
Authors: Hägg K. , Persson T. , Söderman O. , Persson K. M. 
Published: May 2020

Abstract
The natural organic matter (NOM) and color content of surface waters are increasingly becoming an issue for artificial groundwater recharge plants. Water from Lake Bolmen, in southern Sweden, had in 2017 an annual average NOM and color content of 8.6 mg/L total organic carbon (TOC) and 57 mg Pt/L respectively, and values ranging from 7.8 to 9.6 mg/L TOC and 50–70 mg Pt/L. Since water from Lake Bolmen will be used at Vomb Water Works, an artificial groundwater recharge plant, the high NOM-content of Lake Bolmen must be reduced prior to aquifer recharge. From experiences of full-scale operations of chemical flocculation, lamella sedimentation and rapid sand filtration using ferric chloride, three different pre-treatment methods were proposed; conventional precipitation, stand-alone direct precipitation before ultrafiltration (UF), and conventional precipitation with ultrafiltration after lamella sedimentation. In this study, a hollow fiber membrane (MWCO of 150 kDa) was used in different configurations during a 15 months pilot trial. The results showed the possibility to reduce NOM equal to conventional precipitation when a stable net-flux of 40 and 70 L/(m2· h) was used for direct precipitation before UF and conventional precipitation with UF, respectively. This paper presents these treatment methods and evaluates their viability as full-scale treatment steps.
Selenate and selenite uptake, accumulation and toxicity in Lemna minuta
Authors: Li Jun , Loi Giovanni , Otero-Gonzalez Lila , Laing Gijs Du , Ferrer Ivet , Lens Piet N. L. 
Published: May 2020

Abstract
The kinetics of Se uptake and toxicity to Lemna were studied over a period of 14 days of exposure to Se(IV) or Se(VI). The growth of Lemna stopped immediately after exposure to 5.0 mg/L of Se(IV) or Se(VI). The content of chlorophyll and phaeopigments of Lemna exposed to 5.0 mg/L of Se(IV) was two to three times less than in the control after 3 d exposure. Lemna took up Se rapidly within the first 3 d. The Se content in Lemna along with the exposure time fitted well the two-compartment and the hyperbolic model, which demonstrates that the mechanism of Se(IV) and Se(VI) uptake in Lemna is not only through passive diffusion, but also through other processes such as ion channel proteins or transporters. The kinetic bioconcentration factors (BCFs) were 231 and 42 for 0.5 mg/L Se(IV) and Se(VI) exposure, respectively. The uptake rate of Lemna reached 263 mg/kg/d and 28 mg/kg/d in the Se(IV) and Se(VI) treatments, respectively. This study showed that Se(IV) has a faster accumulation rate than Se(VI), but a higher toxicity, indicating Lemna could be a good candidate to remove Se(IV) from water, producing Se-enriched biomass which may eventually also be considered for use as Se-enriched feed supplement or fertilizer.