bluuebunny/crossref_metadata_2025_split huggingface dataset:
Distinct role of 5’UTR sequences in dendritic trafficking of BDNF mRNA: additional mechanisms for the BDNF splice variants spatial code.
Authors: Colliva Andrea , Tongiorgi Enrico 
Published: July 2020

Abstract
The neurotrophin Brain-derived neurotrophic factor (BDNF) is encoded by multiple bipartite transcripts. Each BDNF transcript is composed by one out of eleven alternatively spliced exons containing the 5’untranslated region (UTR), and one common exon encompassing the coding sequence (CDS) and the 3’UTR with two variants (short and long). In neurons, BDNF mRNA variants have a distinct subcellular distribution, constituting a “spatial code”, with exon 1, 3, 5, 7 and 8 located in neuronal somata, exon 4 extending into proximal dendrites, and exon 2 and 6 reaching distal dendrites. We previously showed that the CDS encodes constitutive dendritic targeting signals (DTS) and that both the 3’UTR-short and the 3’UTR-long contain activity-dependent DTS. However, the role of individual 5’UTR exons in mRNA sorting remains unclear. Here, we tested the ability of each different BDNF 5'UTRs to affect the subcellular localization of the green fluorescent protein (GFP) reporter mRNA. We found that exon 2 splicing isoforms (2a, 2b, and 2c) induced a constitutive dendritic targeting of the GFP reporter mRNA towards distal dendritic segments. The other isoforms did not affect GFP-mRNA dendritic trafficking. Though a bioinformatic analysis, we identified five unique cis-elements in exon 2a, 2b, and 2c which might contribute to building a DTS. This study provides additional information on the mechanism regulating the cellular sorting of BDNF mRNA variants.
Adaptive Multi-Modal Fusion Instance Segmentation for CAEVs in Complex Conditions: Dataset, Framework and Verifications
Authors: Peng Pai , Geng Keke , YIN Guodong , Lu Yanbo , Zhuang Weichao , Liu Shuaipeng 
Published: July 2020

Abstract
This paper aims to develop an end-to-end sharpening mixture of experts (SMoE) fusion framework to improve the robustness and accuracy of the perception systems for CAEVs in complex illumination and weather conditions. Three original contributions make our work distinctive from the existing relevant literature. First, we introduce the Complex KITTI dataset which consists of 7481 pairs of modified KITTI RGB images and the generated LiDAR dense depth maps, this dataset is fine annotated in instance-level with our proposed semi-automatic annotation method. Second, the SMoE fusion approach is devised to adaptively learn the robust kernels from complementary modalities. Finally, we implement comprehensive comparative experiments, the results show that our proposed SMoE framework yield significant improvements over the other fusion techniques in adverse environmental conditions.
Dose Effect of Caffeine Ingestion on Cardiac Parasympathetic Reactivation Following an Acute Bout of Anaerobic Performance Exercise in Recreational Athletes
Authors: Sarshin Amir , Naderi Alireza , Cruz Carlos Janssen Gomes da , Feizolahi Foad , Forbes Scott C. , Candow Darren G. , Mohammadgholian Ebrahim , Amiri Mehrdad , Jafari Naghmeh , Rahimi Alireza , Alijani Eidi , Earnest Conrad P. 
Published: July 2020

Abstract
Background: To examine the dose effects of caffeine ingestion on autonomic reactivation following anaerobic exercise. Methods: Recreationally active males (N=20; 24±2y) participated in a randomized, double-blind, placebo-controlled, crossover study where participants ingested: (1) Control (CON; no supplement), (2) a non-caffeinated placebo (PLA), (3) 3-mg∙kg-1 of caffeine (CAF3) or (4) 6-mg∙kg-1 of caffeine (CAF6) prior to a Wingate testing. Parasympathetic (lnRMSSD, primary outcome) and global HRV (lnSDNN, secondary outcome) were assessed at rest (i.e., pre-ingestion), 45-min post-ingestion, and 5-min and 35-min post-exercise recovery. We used a GLM to assess mean (95% CI) changes from pre-ingestion baseline.Results: Overall, we observed a significant trend for lnRMSSD and lnSDNN (both, p=0.001, ηp2=0.745). Forty-five minutes after treatment ingestion, we observed a significant increase in lnRMSSD for CAF3 (0.15ms, 95%CI, 0.07,0.24) and CAF6 (0.16ms, 95%CI, 0.06,0.25), both being significant (both, p<0.004) vs. CON (-0.02ms, 95%CI, -0.09,0.04). Five-minutes after exercise, all treatments demonstrated significant declines in lnRMSSD vs. baseline (all, p<0.001). After 35-min of recovery, lnRMSSD returned to a levels not significantly different than baseline for CAF3 (0.03ms, 95%CI, -0.05, 0.12) and CAF6 (-0.03ms, 95%CI, -0.17, 0.10), while PLA (-0.16ms, 95%CI, -0.25, -0.06) and CON (-0.17ms, 95%CI, -0.28, -0.07) treatments remained significantly depressed. A similar pattern was also observed for SDNNConclusion: Caffeine ingestion increases resting cardiac autonomic modulation and accelerates post-exercise autonomic recovery after a bout of anaerobic exercise test in recreationally active young men. However, no differences between caffeine doses on cardiac autonomic reactivity were observed.
The Prognosis of Eighth Edition TNM Staging System IB (Invasive Component Size ≤ 2 cm) Non–Small Cell Lung Cancer After Sublobar Resection
Authors: Choi Si Young , Moon Mi Hyoung , Moon Youngkyu 
Published: July 2020

Abstract
Background. Sublobar resection is sometimes performed as a surgical treatment for small peripheral tumors. However, there is a question about whether sublobar resection is adequate treatment when visceral pleural invasion is diagnosed postoperatively. The purpose of this study was to evaluate the prognosis of patients with small-sized stage IB non–small cell lung cancer (NSCLC) after sublobar resection.Methods. From January 2010 to December 2018, 227 consecutive patients with eighth edition TNM stage IB NSCLC (per the joint staging system of the International Association for the Study of Lung Cancer and the American Joint Committee on Cancer) underwent curative surgery. The clinicopathological characteristics and prognosis were compared between the sublobar resection group and the lobectomy group. The sublobar resection group included only small-sized (invasive component size ≤ 2 cm) NSCLC.Results. In all study patients, clinicopathological characteristics between the sublobar resection and lobectomy groups were not different except in maximum standardized uptake value and invasive component size. The 5-year recurrence-free survival rate was 80.7% after sublobar resection and 73.4% after lobectomy (P = .349). The 5-year overall survival rate was 87.3% after sublobar resection and 84.8% after lobectomy (P = .503). In patients with small-sized NSCLC, the clinicopathological characteristics were not different between the sublobar resection group and the lobectomy group. The 5-year recurrence-free survival rate was 80.7% after sublobar resection and 72.3% after lobectomy (P = .417). The 5-year overall survival rate was 87.3% after sublobar resection and 91.2% after lobectomy (P = .956). Sublobar resection was not a risk factor for recurrence in the multivariate analysis.Conclusions. The prognosis of sublobar resection in patients with small-sized stage IB NSCLC was comparable with lobectomy. Thus, additional completion lobectomy may not be essential in this setting, despite postoperative upstaging from T1 to T2a.
PD-1, TIM-3 and LAG-3 Expression in T Cells in a Patient with Recurrent Ossifying Fibroma. A Case Report.
Authors: García-Muñoz Alejandro , Nieto-Velázquez Nayeli Goreti , Damian-Morales Gabriela , Liceaga-Escalera Carlos , Montoya-Perez Luis Alberto , Cruz-Vélez Madeleine , Gómez-Hernández Pabel Antonio , Trejo-Iriarte Cynthia , Pastelin-Palacios Rodolfo , Moreno-Eutimio Mario Adán 
Published: July 2020

Abstract
Background: Central ossifying fibroma is a benign, slow-growing tumor of mesenchymal origin with a predilection for the mandibular premolar and molar areas. The immunophenotype of T cells involved in the antitumor response against this benign tumor is unknown.Case presentation: In this case report, we described a case of a 48-year-old woman presenting with a very large recurrent ossifying fibroma in the mandible, which was successfully treated with hemimaxillectomy. In addition, we evaluated the expression of programmed cell death protein-1 (PD-1), lymphocyte activation gene-3 (LAG-3), T cell immunoglobulin and mucin-domain containing-3 (TIM-3), cytotoxic T lymphocyte-associated antigen-4 (CTLA-4), CD69 (activation inducer molecule) and CD25 (α chain of the high-affinity IL-2 receptor) in T cell populations from the tumor and peripheral blood of this uncommon lesion.Conclusions: The patient presented recurrent ossifying fibroma, and the tumor-infiltrating and peripheral blood T cells showed expression of PD-1, LAG-3, and TIM-3, suggesting an exhausted T cell response.
Measuring Greenery Supportiveness in Park for Physical Activity Using Smartphone Panoramic Image and a Green Vegetation Extraction Index
Authors: Yu Xinyang , Huo Wenqian , Niu Beibei , Zhao Maosen 
Published: July 2020

Abstract
Physical activity in urban park contributes substantial health benefits for people. However, due to the absence of profile image systems, objective assessment of greenery supportiveness in park from people’s view is rare or even nonexistent. Manager and planner’s ability to manage and plan park landscapes effectively and efficiently is, therefore, limited. Typical smartphone, such as iPhone, has been widespread used to facilitate daily life. Image captured using panoramic mode in smartphone may be an alternative that can provide profile views of park landscape and greenery, yet no research seems available in literature. We investigated the feasibility of typical smartphone captured panoramic image and proposed a green vegetation extraction index (GVEI) for quantitatively depicting and monitoring the park greenery. A five-kilometer loop road network in Homestead, Florida, whose length and width are similar to the park trail, was selected as the test bed. The iPhone Xs was operated to take panoramic images of one hundred randomly selected investigation sites. Google Street View panoramic images acquired in the identical sites were downloaded and processed as comparison. The results demonstrated that the smartphone panoramic image is time-sensitive and well suited for assessing the trail-level greenery. The GVEI is an objective measurement indicator of trailside greenery. The smartphone panoramic image in combination with GVEI can be used to better understand the greenery supportiveness for physical activity and guide greenery planning in urban park.
Economic Fluctuations and Urban-rural Differences in Educational Inequalities in Mortality in Three Baltic Countries and Finland in 2000–2015: A Register-based Study
Authors: Leinsalu Mall , Baburin Aleksei , Jasilionis Domantas , Krumins Juris , Martikainen Pekka , Stickley Andrew 
Published: July 2020

Abstract
We examined urban-rural differences in educational inequalities in mortality in three Baltic countries and Finland in the context of macroeconomic changes. Educational inequalities among 30–74 year olds were examined in 2000–2003, 2004–2007, 2008–2011 and 2012–2015 using census-linked longitudinal mortality data. We estimated age-standardized mortality rates and the relative and slope index of inequality. Overall mortality rates were larger in rural areas except among Finnish women. Relative educational inequalities in mortality were often larger in urban areas among men but in rural areas among women. Absolute inequalities were mostly larger in rural areas. Between 2000–2003 and 2012–2015 relative inequalities increased in most countries while absolute inequalities decreased except in Lithuania. In the Baltic countries the changes in both relative and absolute inequalities were more favourable in urban areas; in Finland they were more favourable in rural areas. The overall pattern changed during the reccessionary period between 2004–2007 and 2008–2011 when relative inequalities often diminished or the increase slowed, while the decrease in absolute inequalities accelerated with larger improvements observed in urban areas. Despite substantial progress in reducing overall mortality rates in both urban and rural areas in all countries, low educated men and women in rural areas in the Baltic countries are becoming increasingly disadvantaged in terms of mortality reduction.
Anaotmical variability in the position of cystic artery and its affect on surgical approach during laparoscopic cholecystectomy
Authors: Wasi Muhammad Samir Irfan , Fateh Omer , Bukhari Syed Muhammad Abdullah 
Published: July 2020

Abstract
Background: The laparoscopic view of extrahepatic biliary tract and cystic artery is different anatomically from open approach. Consequently iatrogenic injuries due to inadverent damage to cystic artery are not uncommon. These complications can be prevented by careful dissection in Calots triangle and better knowledge of laparoscopic anatomy of cystic artery and its variations. The aim of this study is to establish the prevalence of variation in position of cystic artery in relation to cystic duct in Asia’s largest slum area. This will help identify the safe area for dissecting peritoneum in Calots triangle and thus help young surgeons overcome the long learning curve associated with laparoscopy.Methods: During a 2 year period from 2018-2019, 192 laparoscopic cholecystectomies that were performed at a tertiary care hospital were studied. Patients above the age of 70 years, pregnant females, patients with history of previous abdominal surgery and the cases of conversion from laparoscopic to open cholecystectomy were excluded from the study. Cystic artery was divided into four groups based on its relative position to cystic duct. It includes superomedial, superolateral, anterior and absent cystic artery relative to the cystic duct.Results: Out of 192 cases of laparoscopic cholecystectomy 174 (90.62%) patients had cystic artery superomedial to cystic duct and 10 (5.208%) had a cystic artery at superolateral position to cystic duct. In 5 (2.6%) patients it was found anterior to cystic duct and in 3 (1.56%) patients it was absent.Conclusions: As the least common position of cystic artery was found to be anterior to cystic duct in our study, it is concluded that blind dissection from anterior side is the safest approach to avoid injury to cystic artery. This technique can help young surgeons overcome the long learning curve associated with laparoscopy.
Impact of Physical Activity and Lifestyle in Pre-infection on the Susceptibility and Prognosis of Infects With COVID-19
Authors: Huang Baozhen , Zhang Shixi , Gong Jiuyu , Niu Yanlin , Zhang Fengjuan , Jiang Lin , Wang Mingming , Cui Jing , Li Weixin , Jin Cheng , Bao Pengtao , Li Diangeng 
Published: July 2020

Abstract
Background

There are very few studies focusing on the relationship between COVID-19 and pre-infection lifestyle. In the absence of effective vaccines and special-effect medicines, it is very meaningful to actively respond to the disease pandemic by improving lifestyle habits.
Methods

This is a multicenter, retrospective cohort study enrolled 431 adult people including 228 normal people and 203 confirmed infects in Wubei, Henan and Shandong Provinces. Questionnaires were used to collect information on physical activity and lifestyle by competent doctors. The univariate logistic regression models and multiple regression models were used in risk factor analysis. Kruskal-Wallis H test were used to test the correlation.
Results

Lifestyle habits including exercise, smoking, sleep and physical activity can significantly affect the probability of getting COVID-19(P < 0.05). The MET (Metabolic Equivalent) intensity classification and sleep status are found to be the potential influencing factors of prognosis in both all infects and symptomatic patients. In all infects, taking the high MET intensity level as a reference, inpatient days would increase by 1.812 times (95% CI: 0.887–3.701) with no significance when the level is moderate (P > 0.05) and significantly increase by 6.674 times (95% CI: 1.613–27.613) when the level is low (P < 0.05). Kruskal-Wallis H test results showed moderate activity MET*min promoted shorter hospital stay (P < 0.05) mainly.
Conclusions

Sleep status and physical activity influenced the susceptibility and prognosis of COVID-19. Lack of sleep and low MET intensity level may prolong the hospital stay, which means a relatively slow recovery. This encourages the public to have moderate physical activity and adequate sleep to respond to the COVID-19 pandemic actively.
B-Cell Gene Expression and Microbiota Prior Immunization Profile Vaccine Humoral Responsiveness
Authors: Gonçalves Elena , Guillén Yolanda , Lama Javier R , Sanchez Jorge , Brander Christian , Paredes Roger , Combadière Behazine 
Published: July 2020

Abstract
Background: The identification of new biomarkers is essential to making it possible to predict the degree of protection following vaccination. Very few human studies have focused on baseline characteristics including microbiota and gene expression to underlie vaccine immune responsiveness. We investigated the host whole-blood transcriptome and microbiome before vaccination, to assess the likelihood of their involvement in an effective MVA-neutralizing antibody response (MVA-Nab) two months later. Results: We based our analyses on data obtained ­from a randomized clinical study in which participants (n=10) were vaccinated with the MVA-HIV clade B vaccine (MVA-B). Samples were collected at 2-time points prior vaccination (week-2, w0) to study blood transcriptome. Skin wrap (site of vaccination) and stool microbiota were analysed for diversity and abundance (16S rRNAseq). MVA-neutralizing antibody responses were measured at week 8. The levels of MVA-Nab responses were positively correlated with an abundance of Eubacterium in stool and Prevotella in skin. The simultaneous investigation of blood transcriptome and host microbiota before vaccination showed that genus diversity and bacterial abundance at that time correlated significantly with the expression of genes involved in B cell development stages. The combination of gene expression and microbiota makes it possible to forecast strong responders to MVA-B vaccination.Conclusion: To our knowledge, this is the first study integrating host blood gene expression and microbiota before vaccination to predict the intensity of humoral response months later. The genes identified are involved in B cell differentiation might open an avenue of research in this field to optimize vaccination strategies.
Dynamic imaging in pollen morphology
Authors: Nagy Janos , Tálas László , Hajós Márta Novák , Bojté Csilla , Kiss Alexandra , Szűcs Máté , Illés Árpád , Bojtor Csaba , Mousavi Seyed Mohammad Nasir , Domonkos Dávid , Baksa Viktória , Szemán-Nagy Gábor 
Published: July 2020

Abstract
The performance of domestic agriculture and the food industry is constantly increasing. Demand for food is growing on the global market, with domestic food export growing by 10% in 2019. However, there are still reserves in exploiting the growth path and transforming it into income. Efficiency, revenue and profit growth of each product line depends on numerous factors; one of the key tools being the monitoring of the production process, getting to know the production environment accurately, data collecting, database building, development of applications that support automated interventions and decisions, and integration the above tools. The fundamental objective goal of the presented research is to approach the research of maize pollen cells as an agriculturally relevant model organism from a new direction, the long-term (Time-Lapse System) microscopic study of the growth dynamics of the pollen germ tube. In the scope of the research, two industrially and agriculturally important aspects taken into account, which also detected digitally: the growth of the maize pollen germ tube and the viability of the pollen. The research can provide a basis for the more accurate understanding and subsequent study of the effects of different biotic and abiotic stress factors on pollen growth, and may open up new possibilities in the field of digital agricultural biotechnology.
Determinants of hand hygiene practice at critical times among food handlers in educational institutions in Sagnarigu Municipality of Ghana: A cross-sectional study
Authors: Amegah Kingsley E. , Addo Henry O. , Ashinyo Mary E. , Fiagbe Loveland , Akpanya Serene , Akoriyea Samuel K. , Dubik S. Dajaan 
Published: July 2020

Abstract
INTRODUCTION: Food handlers' hands serve as a vehicle for potential foodborne pathogenic contamination which constitutes a public health risk. In Ghana, there are always constant reports of outbreak of foodborne diseases in schools. However, determinants of hand hygiene practice among educational institutions food handlers are little known in Ghana. The study, therefore, aimed to assess the determinants of hand hygiene practice at critical times among educational institutions food handlers in the Sagnarigu Municipality of Ghana.METHODS: This was a cross-sectional survey among educational institutions food handlers in the Sagnarigu Municipality. Two hundred and six food handlers were selected through convenience sampling in the educational institutions. At the same time, structured questions that were developed from previous studies were used to assess food handlers' hand hygiene practice at critical times. Data were analyzed using descriptive statistics, bivariate and multivariate logistic regression models. All variables with their respective confidence interval (95%) and adjusted odds ratios were declared significant at p-value less than 0.05.RESULTS: The hand hygiene practice at critical times among the food handlers was relatively good 66.0% (95% CI: 59.1, 72.5%) and is independently predicted by training on food safety [AOR = 24.39, (95% CI: 2.24 – 245.45) p=0.007], sufficient knowledge of hand hygiene [AOR = 16.34, (95% CI: 3.92 – 68.05) p<0.001] and positive attitude towards hand hygiene [AOR = 82.08, (95% CI: 14.21-474.26) p<0.001]. Only 17.0% of the food handlers demonstrated proper handwashing techniques whereas a good number (85.4%) of the food handlers did not wash their hands after touching money.CONCLUSION: Good hand hygiene practice at critical times was influenced by training on food safety, sufficient knowledge of hand hygiene, and positive attitudes towards hand hygiene. These findings demonstrate the significant role of food handlers' hand hygiene knowledge, attitudes, and training on food safety in improving hand hygiene practices. Concerned stakeholders and organizations should focus on WASH interventions that seek to improve educational institutions food handlers’ knowledge and attitudes towards hand hygiene, coupled with training on food safety.