bluuebunny/crossref_metadata_2025_split huggingface dataset:
Automatic support control of an upper body exoskeleton — Method and validation using the Stuttgart Exo-Jacket
Authors: Singer Raphael , Maufroy Christophe , Schneider Urs 
Published: September 2020

AbstractAlthough passive occupational exoskeletons alleviate worker physical stresses in demanding postures (e.g., overhead work), they are unsuitable in many other applications because of their lack of flexibility. Active exoskeletons that are able to dynamically adjust the delivered support are required. However, the automatic control of support provided by the exoskeleton is still a largely unsolved challenge in many applications, especially for upper limb occupational exoskeletons, where no practical and reliable approach exists. For this type of exoskeletons, a novel support control approach for lifting and carrying activities is presented here. As an initial step towards a full-fledged automatic support control (ASC), the present article focusses on the functionality of estimating the onset of user’s demand for support. In this way, intuitive behavior should be made possible. The combination of movement and muscle activation signals of the upper limbs is expected to enable high reliability, cost efficiency, and compatibility for use in industrial applications. The functionality consists of two parts: a preprocessing—the motion interpretation—and the support detection itself. Both parts were trained with different subjects, who had to move objects. The functionality was validated both in the cases of (A) an unknown subject performing known tasks and (B) a known subject performing unknown tasks. The functionality showed sound results as it achieved a high accuracy ($$ 95\% $$) in training. In addition, the first validation results showed that this functionality is useful for integration in an appropriately adapted ASC and can then enable comfortable working.
Radon Activity in Volcanic Gases of Mt. Etna by Passive Dosimetry
Authors: Terray Luca , Gauthier Pierre‐Jean , Breton Vincent , Giammanco Salvatore , Sigmarsson Olgeir , Salerno Giuseppe , Caltabiano Tommaso , Falvard Alain 
Published: September 2020

AbstractRadon (222Rn) activity in air was measured for about 6 months at the summit of Mt. Etna Central Crater (Sicily) by integrative radon dosimetry at two different heights above ground level (5 cm and 1 m). This technique for air radon monitoring proved operational in the harsh volcanic environment of Mt. Etna summit with a 94% recovery rate of dosimeters. In the southeast sector exposed to the main gas plume, mean radon activity in free air (height 1 m) is significantly higher than the local background and the ground level activity (height 5 cm). The results strongly suggest that the plume is enriched in radon by ≈550 Bq/m3, which has never been evidenced before. Radon activities also reflect soil degassing occurring in the proximity of the crater, with increased ground level activities in zones of enhanced soil fracturing and degassing. Radon measurements also revealed a hot spot in front of the Voragine vent with extraordinary high levels of air activities (26 kBq/m3 at ground level and 8 kBq/m3 in free air). The temporal variation of radon activity was investigated by replacing a few stations half way through the exposure period. The only significant increase was associated with the site located under the main gas plume and correlated with eruptive unrest within the crater. Finally, air radon levels higher than the recommended threshold of 300 Bq/m3 were detected in several zones on the rim and could generate a nonnegligible radiologic dose for workers on the volcano.
Evolution of Magmatism in the New Hebrides Island Arc and in Initial Back‐Arc Rifting, SW Pacific
Authors: Haase K. M. , Gress M. U. , Lima S. M. , Regelous M. , Beier C. , Romer R. L. , Bellon H. 
Published: September 2020

AbstractWe present new geochemical and isotopic data for rock samples from two island arc volcanoes, Erromango and Vulcan Seamount, and from a 500 m thick stratigraphic profile of lava flows exposed on the SW flank of Vate Trough back‐arc rift of the New Hebrides Island Arc (NHIA). The basalts from the SW rift flank of Vate Trough have ages of ~0.5 Ma but are geochemically similar to those erupting along the active back‐arc rift. The weak subduction component in the back‐arc basalts implies formation by decompression melting during early rifting and rifting initiation by tectonic processes rather than by lithosphere weakening by arc magma. Melting beneath Vate Trough is probably caused by chemically heterogeneous and hot mantle that flows in from the North Fiji Basin in the east. The melting zone beneath Vate Trough back‐arc is separate from that of the arc front, but a weak slab component suggests fluid transport from the slab. Immobile incompatible element ratios in South NHIA lavas overlap with those of the Vate Trough depleted back‐arc basalts, suggesting that enriched mantle components are depleted by back‐arc melting during mantle flow. The slab component varies from hydrous melts of subducted sediments in the Central NHIA to fluids from altered basalts in the South NHIA. The volcanism of Erromango shows constant compositions for 5 million years, that is, there is no sign for variable depletion of the mantle or for a change of slab components due to collision of the D'Entrecasteaux Ridge as in lava successions further north.
Inflow Speed Analysis of Interchange Injections in Saturn's Magnetosphere
Authors: Paranicas C. , Thomsen M. F. , Kollmann P. , Azari A. R. , Bader A. , Badman S. V. , Dumont M. , Kinrade J. , Krupp N. , Roussos E. 
Published: September 2020

AbstractDuring its more than 13 years in orbit, the Cassini spacecraft detected a large number of plasma and energetic charged particle injections in Saturn's inner magnetosphere. In the corotating frame of the planet, the plasma contained within an injection moves radially inward with the component particles gaining energy. The highest energy particles in the injection experience stronger gradient‐curvature drifts in the longitudinal direction and can drift out of the main body of the injection. We have used these drift‐out effects to estimate the inflow speed of 20 injections by surveying cases from the available plasma data. We find that the average inflow speed from our sample is 15 km/s, and the values are well distributed between 0 and 50 km/s, with a few higher estimates. We have also computed the radial travel distance of interchange events and found that these are typically one to two Saturn radii. We discuss the implications of these quantifications on our understanding of transport.
Seismo‐Turbidites in Aysén Fjord (Southern Chile) Reveal a Complex Pattern of Rupture Modes Along the 1960 Megathrust Earthquake Segment
Authors: Wils Katleen , Van Daele Maarten , Kissel Catherine , Moernaut Jasper , Schmidt Sabine , Siani Giuseppe , Lastras Galderic 
Published: September 2020

AbstractGrain size analysis and end‐member modeling of a long sediment core from Aysén Fjord (southern Chile) allows to identify over 25 seismo‐turbidites in the last 9,000 years. Considering the shaking intensities required to trigger these turbidites (V½‐VI½), the majority can be related to megathrust earthquakes. Multiple studies in south‐central Chile have aimed at finding traces of giant, tsunamigenic megathrust earthquakes leading to the current 5,500‐year‐long paleoseismological record of the Valdivia segment. However, none of these cover the southern third of the segment. Aysén Fjord allows to fill this data gap and presents the first, crucial paleoseismic data to demonstrate that the 1960 event was not unique for the Valdivia segment, yielding a recurrence rate of 321 ± 116 years in the last two millennia. Moreover, the oldest identified events in Aysén Fjord date back to 9,000 cal years BP and, thus, also extend the regional paleoseismological record in time. We infer a large temporal variability in rupture modes, with successions of full‐segment ruptures alternating with partial and cascading ruptures. The latter seems to significantly postpone the occurrence of another full rupture when consecutively occurring in different parts of the segment. Additionally, one outstanding period of seismic quiescence—during which no megathrust earthquake evidence has been found at any paleoseismic site—occurred after a full rupture in AD ~745 that presents an unusual uplift/subsidence pattern. Such variability makes it highly speculative to anticipate the rupture mode of the next megathrust earthquake along the Valdivia segment.
Identification of disease-causing variants by comprehensive genetic testing with exome sequencing in adults with suspicion of hereditary FSGS
Authors: Braunisch Matthias Christoph , Riedhammer Korbinian Maria , Herr Pierre-Maurice , Draut Sarah , Günthner Roman , Wagner Matias , Weidenbusch Marc , Lungu Adrian , Alhaddad Bader , Renders Lutz , Strom Tim M. , Heemann Uwe , Meitinger Thomas , Schmaderer Christoph , Hoefele Julia 
Published: September 2020

AbstractIn about 30% of infantile, juvenile, or adolescent patients with steroid-resistant nephrotic syndrome (SRNS), a monogenic cause can be identified. The histological finding in SRNS is often focal segmental glomerulosclerosis (FSGS). Genetic data on adult patients are scarce with low diagnostic yields. Exome sequencing (ES) was performed in patients with adult disease onset and a high likelihood for hereditary FSGS. A high likelihood was defined if at least one of the following criteria was present: absence of a secondary cause, ≤25 years of age at initial manifestation, kidney biopsy with suspicion of a hereditary cause, extrarenal manifestations, and/or positive familial history/reported consanguinity. Patients were excluded if age at disease onset was <18 years. In 7/24 index patients with adult disease onset, a disease-causing variant could be identified by ES leading to a diagnostic yield of 29%. Eight different variants were identified in six known genes associated with monogenic kidney diseases. Six of these variants had been described before as disease-causing. In patients with a disease-causing variant, the median age at disease onset and end-stage renal disease was 26 and 38 years, respectively. The overall median time to a definite genetic diagnosis was 9 years. In 29% of patients with adult disease onset and suspected hereditary FSGS, a monogenic cause could be identified. The long delay up to the definite genetic diagnosis highlights the importance of obtaining an early genetic diagnosis to allow for personalized treatment options including weaning of immunosuppressive treatment, avoidance of repeated renal biopsy, and provision of accurate genetic counseling.
Chromosome 1q21.2 and additional loci influence risk of spontaneous coronary artery dissection and myocardial infarction
Authors: Saw Jacqueline , Yang Min-Lee , Trinder Mark , Tcheandjieu Catherine , Xu Chang , Starovoytov Andrew , Birt Isabelle , Mathis Michael R. , Hunker Kristina L. , Schmidt Ellen M. , Jackson Linda , Fendrikova-Mahlay Natalia , Zawistowski Matthew , Brummett Chad M. , Zoellner Sebastian , Katz Alexander , Coleman Dawn M. , Swan Kirby , O’Donnell Christopher J. ,   Million Veteran Program, Assimes Themistocles L. , O’Donnell Christopher J. , Zhou Xiang , Li Jun Z. , Gornik Heather L. , Assimes Themistocles L. , Stanley James C. , Brunham Liam R. , Ganesh Santhi K. 
Published: September 2020

AbstractSpontaneous coronary artery dissection (SCAD) is a non-atherosclerotic cause of myocardial infarction (MI), typically in young women. We undertook a genome-wide association study of SCAD (Ncases = 270/Ncontrols = 5,263) and identified and replicated an association of rs12740679 at chromosome 1q21.2 (Pdiscovery+replication = 2.19 × 10−12, OR = 1.8) influencing ADAMTSL4 expression. Meta-analysis of discovery and replication samples identified associations with P < 5 × 10−8 at chromosome 6p24.1 in PHACTR1, chromosome 12q13.3 in LRP1, and in females-only, at chromosome 21q22.11 near LINC00310. A polygenic risk score for SCAD was associated with (1) higher risk of SCAD in individuals with fibromuscular dysplasia (P = 0.021, OR = 1.82 [95% CI: 1.09–3.02]) and (2) lower risk of atherosclerotic coronary artery disease and MI in the UK Biobank (P = 1.28 × 10−17, HR = 0.91 [95% CI :0.89–0.93], for MI) and Million Veteran Program (P = 9.33 × 10−36, OR = 0.95 [95% CI: 0.94–0.96], for CAD; P = 3.35 × 10−6, OR = 0.96 [95% CI: 0.95–0.98] for MI). Here we report that SCAD-related MI and atherosclerotic MI exist at opposite ends of a genetic risk spectrum, inciting MI with disparate underlying vascular biology.
Pan-cancer study detects genetic risk variants and shared genetic basis in two large cohorts
Authors: Rashkin Sara R. , Graff Rebecca E. , Kachuri Linda , Thai Khanh K. , Alexeeff Stacey E. , Blatchins Maruta A. , Cavazos Taylor B. , Corley Douglas A. , Emami Nima C. , Hoffman Joshua D. , Jorgenson Eric , Kushi Lawrence H. , Meyers Travis J. , Van Den Eeden Stephen K. , Ziv Elad , Habel Laurel A. , Hoffmann Thomas J. , Sakoda Lori C. , Witte John S. 
Published: September 2020

AbstractDeciphering the shared genetic basis of distinct cancers has the potential to elucidate carcinogenic mechanisms and inform broadly applicable risk assessment efforts. Here, we undertake genome-wide association studies (GWAS) and comprehensive evaluations of heritability and pleiotropy across 18 cancer types in two large, population-based cohorts: the UK Biobank (408,786 European ancestry individuals; 48,961 cancer cases) and the Kaiser Permanente Genetic Epidemiology Research on Adult Health and Aging cohorts (66,526 European ancestry individuals; 16,001 cancer cases). The GWAS detect 21 genome-wide significant associations independent of previously reported results. Investigations of pleiotropy identify 12 cancer pairs exhibiting either positive or negative genetic correlations; 25 pleiotropic loci; and 100 independent pleiotropic variants, many of which are regulatory elements and/or influence cross-tissue gene expression. Our findings demonstrate widespread pleiotropy and offer further insight into the complex genetic architecture of cross-cancer susceptibility.
Structures of Echovirus 30 in complex with its receptors inform a rational prediction for enterovirus receptor usage
Authors: Wang Kang , Zhu Ling , Sun Yao , Li Minhao , Zhao Xin , Cui Lunbiao , Zhang Li , Gao George F. , Zhai Weiwei , Zhu Fengcai , Rao Zihe , Wang Xiangxi 
Published: September 2020

AbstractReceptor usage that determines cell tropism and drives viral classification closely correlates with the virus structure. Enterovirus B (EV-B) consists of several subgroups according to receptor usage, among which echovirus 30 (E30), a leading causative agent for human aseptic meningitis, utilizes FcRn as an uncoating receptor. However, receptors for many EVs remain unknown. Here we analyzed the atomic structures of E30 mature virion, empty- and A-particles, which reveals serotype-specific epitopes and striking conformational differences between the subgroups within EV-Bs. Of these, the VP1 BC loop markedly distinguishes E30 from other EV-Bs, indicative of a role as a structural marker for EV-B. By obtaining cryo-electron microscopy structures of E30 in complex with its receptor FcRn and CD55 and comparing its homologs, we deciphered the underlying molecular basis for receptor recognition. Together with experimentally derived viral receptor identifications, we developed a structure-based in silico algorithm to inform a rational prediction for EV receptor usage.
Protein signatures of seminal plasma from bulls with contrasting frozen-thawed sperm viability
Authors: Gomes Fabio P. , Park Robin , Viana Arabela G. , Fernandez-Costa Carolina , Topper Einko , Kaya Abdullah , Memili Erdogan , Yates John R. , Moura Arlindo A. 
Published: September 2020

AbstractThe present study investigated the seminal plasma proteome of Holstein bulls with low (LF; n = 6) and high (HF; n = 8) sperm freezability. The percentage of viable frozen-thawed sperm (%ViableSperm) determined by flow cytometry varied from -2.2 in LF to + 7.8 in HF bulls, as compared to the average %ViableSperm (54.7%) measured in an 860-sire population. Seminal proteins were analyzed by label free mass spectrometry, with the support of statistical and bioinformatics analyses. This approach identified 1,445 proteins, associated with protein folding, cell–cell adhesion, NADH dehydrogenase activity, ATP-binding, proteasome complex, among other processes. There were 338 seminal proteins differentially expressed (p < 0.05) in LF and HF bulls. Based on multivariate analysis, BSP5 and seminal ribonuclease defined the HF phenotype, while spermadhesin-1, gelsolin, tubulins, glyceraldehyde-3-phosphate dehydrogenase, calmodulin, ATP synthase, sperm equatorial segment protein 1, peroxiredoxin-5, secretoglobin family 1D and glucose-6-phosphate isomerase characterized the LF phenotype. Regression models indicated that %ViableSperm of bulls was related to seminal plasma peroxiredoxin-5, spermadhesin-1 and the spermadhesin-1 × BSP5 interaction (R2 = 0.84 and 0.79; p < 0.05). This report is the largest dataset of bovine seminal plasma proteins. Specific proteins of the non-cellular microenvironment of semen are potential markers of sperm cryotolerance.
Cystathionine β-synthase is involved in cysteine biosynthesis and H2S generation in Toxoplasma gondii
Authors: Conter Carolina , Fruncillo Silvia , Fernández-Rodríguez Carmen , Martínez-Cruz Luis Alfonso , Dominici Paola , Astegno Alessandra 
Published: September 2020

AbstractCystathionine β-synthase (CBS) catalyzes the condensation of serine and homocysteine to water and cystathionine, which is then hydrolyzed to cysteine, α-ketobutyrate and ammonia by cystathionine γ-lyase (CGL) in the reverse transsulfuration pathway. The protozoan parasite Toxoplasma gondii, the causative agent of toxoplasmosis, includes both CBS and CGL enzymes. We have recently reported that the putative T. gondii CGL gene encodes a functional enzyme. Herein, we cloned and biochemically characterized cDNA encoding CBS from T. gondii (TgCBS), which represents a first example of protozoan CBS that does not bind heme but possesses two C-terminal CBS domains. We demonstrated that TgCBS can use both serine and O-acetylserine to produce cystathionine, converting these substrates to an aminoacrylate intermediate as part of a PLP-catalyzed β-replacement reaction. Besides a role in cysteine biosynthesis, TgCBS can also efficiently produce hydrogen sulfide, preferentially via condensation of cysteine and homocysteine. Unlike the human counterpart and similar to CBS enzymes from lower organisms, the TgCBS activity is not stimulated by S-adenosylmethionine. This study establishes the presence of an intact functional reverse transsulfuration pathway in T. gondii and demonstrates the crucial role of TgCBS in biogenesis of H2S.
Characterization of glycerol-3-phosphate acyltransferase 9 (AhGPAT9) genes, their allelic polymorphism and association with oil content in peanut (Arachis hypogaea L.)
Authors: Lv Yuying , Zhang Xiurong , Luo Lu , Yang Hui , Li Pinghua , Zhang Kun , Liu Fengzhen , Wan Yongshan 
Published: September 2020

AbstractGPAT, the rate-limiting enzyme in triacylglycerol (TAG) synthesis, plays an important role in seed oil accumulation. In this study, two AhGPAT9 genes were individually cloned from the A- and B- genomes of peanut, which shared a similarity of 95.65%, with 165 site differences. The overexpression of AhGPAT9 or the knock-down of its gene expression increased or decreased the seed oil content, respectively. Allelic polymorphism analysis was conducted in 171 peanut germplasm, and 118 polymorphic sites in AhGPAT9A formed 64 haplotypes (a1 to a64), while 94 polymorphic sites in AhGPAT9B formed 75 haplotypes (b1 to b75). The haplotype analysis showed that a5, b57, b30 and b35 were elite haplotypes related to high oil content, whereas a7, a14, a48, b51 and b54 were low oil content types. Additionally, haplotype combinations a62/b10, a38/b31 and a43/b36 were associated with high oil content, but a9/b42 was a low oil content haplotype combination. The results will provide valuable clues for breeding new lines with higher seed oil content using hybrid polymerization of high-oil alleles of AhGPAT9A and AhGPAT9B genes.
Identification of mycobacteriophage toxic genes reveals new features of mycobacterial physiology and morphology
Authors: Ko Ching-Chung , Hatfull Graham F. 
Published: September 2020

AbstractDouble-stranded DNA tailed bacteriophages typically code for 50–200 genes, of which 15–35 are involved in virion structure and assembly, DNA packaging, lysis, and DNA metabolism. However, vast numbers of other phage genes are small, are not required for lytic growth, and are of unknown function. The 1,885 sequenced mycobacteriophages encompass over 200,000 genes in 7,300 distinct protein ‘phamilies’, 77% of which are of unknown function. Gene toxicity provides potential insights into function, and here we screened 193 unrelated genes encoded by 13 different mycobacteriophages for their ability to impair the growth of Mycobacterium smegmatis. We identified 45 (23%) mycobacteriophage genes that are toxic when expressed. The impacts on M. smegmatis growth range from mild to severe, but many cause irreversible loss of viability. Expression of most of the severely toxic genes confers altered cellular morphologies, including filamentation, polar bulging, curving, and, surprisingly, loss of viability of one daughter cell at division, suggesting specific impairments of mycobacterial growth. Co-immunoprecipitation and mass spectrometry show that toxicity is frequently associated with interaction with host proteins and alteration or inactivation of their function. Mycobacteriophages thus present a massive reservoir of genes for identifying mycobacterial essential functions, identifying potential drug targets and for exploring mycobacteriophage physiology.
A realistic fish-habitat dataset to evaluate algorithms for underwater visual analysis
Authors: Saleh Alzayat , Laradji Issam H. , Konovalov Dmitry A. , Bradley Michael , Vazquez David , Sheaves Marcus 
Published: September 2020

AbstractVisual analysis of complex fish habitats is an important step towards sustainable fisheries for human consumption and environmental protection. Deep Learning methods have shown great promise for scene analysis when trained on large-scale datasets. However, current datasets for fish analysis tend to focus on the classification task within constrained, plain environments which do not capture the complexity of underwater fish habitats. To address this limitation, we present DeepFish as a benchmark suite with a large-scale dataset to train and test methods for several computer vision tasks. The dataset consists of approximately 40 thousand images collected underwater from 20 habitats in the marine-environments of tropical Australia. The dataset originally contained only classification labels. Thus, we collected point-level and segmentation labels to have a more comprehensive fish analysis benchmark. These labels enable models to learn to automatically monitor fish count, identify their locations, and estimate their sizes. Our experiments provide an in-depth analysis of the dataset characteristics, and the performance evaluation of several state-of-the-art approaches based on our benchmark. Although models pre-trained on ImageNet have successfully performed on this benchmark, there is still room for improvement. Therefore, this benchmark serves as a testbed to motivate further development in this challenging domain of underwater computer vision.