bluuebunny/crossref_metadata_2025_split huggingface dataset:
Kinetics characterization of ASXL1/2-mediated allosteric regulation of BAP1 deubiquitinase
Authors: Peng Hongzhuang , Cassel Joel , McCracken Daniel S. , Prokop Jeremy W. , Collop Paul R. , Polo Alexander , Joshi Surbhi , Mandell Jacob P. , Ayyanathan Kasirajan , Hinds David , Malkowicz S. Bruce , Harbour J. William , Bowcock Anne M. , Salvino Joseph , Kennedy Eileen J. , Testa Joseph R. , Rauscher Frank J. 
Published: February 2020

AbstractBAP1 is a ubiquitin hydrolase whose deubiquitinase activity is mediated by polycomb group-like protein ASXL2. Cancer-related mutations/deletions ofBAP1lead to loss-of-function either by directly targeting the catalytic (UCH) or ULD domains of BAP1, the latter disrupts binding to ASXL2, an obligate partner for BAP1 enzymatic activity. However, the biochemical and biophysical properties of the domains involved in forming the enzymatically active complex are unknown. Here we investigate the molecular dynamics, kinetics and stoichiometry of these interactions. We demonstrate that the BAP1 and ASXL2 domain/proteins or protein complexes produced in either bacteria or baculovirus are structurally and functionally active. The interaction between BAP1 and ASXL2 is direct, specific, and stable toin vitrobiochemical and biophysical manipulations as detected by isothermal titration calorimetry, GST association, and optical biosensor assays. Association of the ASXL2-AB box greatly stimulates BAP1 deubiquitinase activity. A stable ternary complex can be formed comprised of the BAP1-UCH, BAP1-ULD, and ASXL2-AB domains. Binding of the BAP1-ULD domain to the ASXL2-AB box is rapid, with fast association and slow dissociation rates. Stoichiometric analysis revealed that one molecule of the ULD domain directly interacts with one molecule of the AB Box. Real-time kinetics analysis of ULD/AB protein complex to the UCH domain of BAP1, based on SPR, indicated that formation of the ULD/AB complex with the UCH domain is a single-step event with fast association and slow dissociation rates. These structural and dynamic parameters implicate the possibility for future small-molecule approaches to reactivate latent wild-type UCH activity in BAP-mutant malignancies.
ANME-1 archaea drive methane accumulation and removal in estuarine sediments
Authors: Kevorkian Richard , Callahan Sean , Winstead Rachel , Lloyd Karen G. 
Published: February 2020

AbstractUncultured members of the Methanomicrobia called ANME-1 perform the anaerobic oxidation of methane (AOM) through a process that uses much of the methanogenic pathway. It is unknown whether ANME-1 obligately perform AOM, or whether some of them can perform methanogenesis when methanogenesis is exergonic. Most marine sediments lack advective transport of methane, so AOM occurs in the sulfate methane transition zone (SMTZ) where sulfate-reducing bacteria consume hydrogen produced by fermenters, making hydrogenotrophic methanogenesis exergonic in the reverse direction. When sulfate is depleted deeper in the sediments, hydrogen accumulates making hydrogenotrophic methanogenesis exergonic, and methane accumulates in the methane zone (MZ). In White Oak River estuarine sediments, we found that ANME-1 comprised 99.5% of 16S rRNA genes from amplicons and 100% of 16S rRNA genes from metagenomes of the Methanomicrobia in the SMTZ and 99.9% and 98.3%, respectively, in the MZ. Each of the 16 ANME-1 OTUs (97% similarity) had peaks in the SMTZ that coincided with peaks of putative sulfate-reducing bacteriaDesulfatiglans sp.and SEEP-SRB1. In the MZ, ANME-1, but no putative sulfate-reducing bacteria or cultured methanogens, increased with depth. Using publicly available data, we found that ANME-1 was the only group expressing methanogenic genes during both net AOM and net methanogenesis in an enrichment. The commonly-held belief that ANME-1 perform AOM is based on the fact that they dominate natural settings and enrichments where net AOM is measured. We found that ANME-1 also dominate natural settings and enrichment where net methanogenesis is measured, so we conclude that ANME-1 perform methane production. Alternating between AOM and methanogenesis, either in a single ANME-1 cell or between different subclades with similar 16S rRNA sequences of ANME-1, may confer a competitive advantage, explaining the predominance of low-energy adapted ANME-1 in methanogenic sediments worldwide.Abstract ImportanceLife may operate differently at very low energy levels. Natural populations of microbes that make methane survive on some of the lowest energy yields of all life. From all available data, we infer that these microbes alternate between methane production and oxidation, depending on which process is energy-yielding in the environment. This means that much of the methane produced naturally in marine sediments occurs through an organism that is also capable of destroying it under different circumstances.
Antinociceptive modulation by the adhesion-GPCR CIRL promotes mechanosensory signal discrimination
Authors: Dannhäuser Sven , Lux Thomas J. , Hu Chun , Selcho Mareike , Chen Jeremy Tsung-Chieh , Ehmann Nadine , Sachidanandan Divya , Pawlak Matthias , Langenhan Tobias , Soba Peter , Rittner Heike , Kittel Robert J. 
Published: February 2020

ABSTRACTAdhesion-type G protein-coupled receptors (aGPCRs) participate in a vast range of physiological processes. Correspondingly, these receptors are associated with diverse human diseases, such as developmental disorders, defects of the nervous system, allergies and cancer. Several aGPCRs have recently been linked to mechanosensitive functions suggesting that processing of mechanical stimuli may be a common feature of this receptor family. CIRL (ADGRL/Latrophilin, LPHN), one of the oldest members of the aGPCR family, sensitizes sensory responses of larvalDrosophilato gentle touch and sound by amplifying mechanosensory signal transduction in low-threshold mechanoreceptors (Scholz et al., 2015; 2017). In the present study, we show thatCirlis also expressed in high-threshold mechanical nociceptors where it adjusts nocifensive behaviour under physiological and pathophysiological conditions. Optogeneticin vivoexperiments indicate that CIRL quenches cAMP levels in both mechanosensory submodalities. However, contrasting its effect in touch sensitive neurons, CIRL dampens the response of nociceptors to mechanical stimulation. Consistent with this finding, rat nociceptors display a drop inCirl1expression during allodynia. Taken together, these results demonstrate that CIRL exerts opposing modulatory functions in low-threshold mechanosensors and high-threshold nociceptors. This intriguing bipolar action likely facilitates the separation of mechanosensory signals carrying different physiological information.
Quantitative LCMS for ceramides/dihydroceramides: pregnancy baseline biomarkers and potential metabolic messengers
Authors: Huang Qianyang , Hao Shiying , Yao Xiaoming , You Jin , Li Xiao , Lai Donghai , Han Chunle , Schilling James , Hwa Kuo Yuan , Thyparambil Sheeno , Whitin John , Cohen Harvey J. , Chubb Henry J. , Ceresnak Scott R. , McElhinney Doff B. , Shaw Gary M. , Stevenson David K. , Sylvester Karl G. , Ling Xuefeng B. 
Published: February 2020

AbstractCeramides and dihydroceramides are sphingolipids that present in abundance at the cellular membrane of eukaryotes. Although their metabolic dysregulation has been implicated in many diseases, our knowledge about circulating ceramide changes during the pregnancy remains limited. In this study, we present the development and validation of a high-throughput liquid chromatography-tandem mass spectrometric (LC/MS/MS) method for simultaneous quantification of 16 ceramides and 10 dihydroceramides in human serum within 5 mins by using stable isotope-labeled ceramides as internal standards (ISs). This method employs a protein precipitation method for high throughput sample preparation, reverse phase isocratic elusion for chromatographic separation, and Multiple Reaction Monitoring (MRM) for mass spectrometric detection. To qualify for clinical applications, our assay was validated against the FDA guidelines: the Lower Limit of Quantitation (LLOQ as low as 1 nM), linearity (R2>0.99), precision (Coefficient of Variation<15%), accuracy (Percent Error<15%), extraction recovery (>90%), stability (>85%), and carryover (<0.1%). With enhanced sensitivity and specificity from this method, we have, for the first time, determined the serological levels of ceramides and dihydroceramides to reveal unique temporal gestational patterns. Our approach could have value in providing insights into disorders of pregnancy.
Financial constraints and marketing investment: evidence from text analysis
Authors: Mishra Sagarika , Ewing Mike T. 
Published: February 2020

Purpose
The purpose of this study to examine the effect of financial constraint on intangible investment because intangible investment provides an overall picture of marketing investment and activity. Intangible investment also plays a significant role in facilitating future sales. Using a new measure of intangible investment (Peters and Taylor, 2017), the authors first establish that intangible investment is positively related with future sales. Then, using a new text-based measure of financial constraint, the authors show that financial constraint has a significant negative effect on future intangible investments after controlling for other factors. Intangible investment has three components. The first is R&D, the second is 30 per cent of selling and general administrative expense (SGA) and the third is other intangibles. The authors find that the negative and significant effect of financial constraint on 30 per cent SGA is stronger. This indicates that financially constrained firms reduce marketing related investments. The authors then considered firm size and found that smaller firms facing financial constraint continue to increase their intangible investments, whereas larger firms reduce their intangible investment. As a robustness test, the authors use advertising expenditure as a measure of promotion related investment and find that financial constraint has a negative effect on advertising spending. The authors then use two traditional measures of financial constraint in their analysis to compare with the new text-based measure.

Design/methodology/approach
The authors use ordinary least squares with cluster robust standard error to conduct their empirical analysis.

Findings
First the authors establish that intangible investment positively affects future sales. Further the authors find that financial constraint negatively affects intangible investment. Moreover, financial constraint negatively affects the brand capital of intangible investment.

Research limitations/implications
The authors did not conduct any industry specific analysis to see how financial constraints affect intangible investment across different industries. Industry specific analysis is important because in some industries/sectors intangibles are clearly more important than in others, so this is an important avenue for future research. It will also be interesting to explore if and how financial constraint has a mediating effect on sales growth via intangible investment and different components of intangibles.

Practical implications
This study identifies another important factor that can negatively affect brand capital investment.

Originality/value
The authors have used a measure of financial constraint and text mined all the annual reports of US firms for the period of 1994-2016 to compute this measure.
“From caged birds to women with wings”
Authors: Rangwala Shenaz , Jayawardhena Chanaka , Saxena Gunjan 
Published: February 2020

Purpose
This study aims to explore consumption practices of new middle-class Indian women to explicate how they are challenging traditional social norms and redefining their identity through their consumption practices.

Design/methodology/approach
A total of 32 semi-structured and photo-elicitation interviews were conducted with new middle-class women between the age group of 23 and 40 years in India.

Findings
This study illustrates how the doing of consumption practices that involve creating, controlling, knowing and transforming is enabling new middle-class Indian women to undo gender disparities embedded in hegemonic patriarchal social order. Also, the study provides new insights into how class and symbolic capital intersect gender to redefine middle-class women’s feminine self.

Research limitations/implications
This study specifically illustrates how new middle-class women are using consumption practices to uplift their position in household; bring about new modes of social interface; and identity expression and a reversal in gender roles.

Practical implications
The conflation of women’s independence with consumerism underlines the need for marketers to position consumer goods in a manner that strengthens women’s self and alleviates cultural perceptions of women as subordinate to men in the household. Indian market has considerable growth potential for publicly visible brands that affirm the elevated social status of women and allow them to effectively demonstrate their capital resources.

Originality/value
An under-researched consumer segment is explored by focusing particularly on the intersection of discourses of women’s individuality with that of their consumption practices. Additionally, pioneering use of photo-elicitation technique coupled with hermeneutic approach enabled to elicit effectively women’s reflections on their behaviours, values and motivations underlying their consumption practices.
Assessing the factorial structure and internal consistency of the mental fitness and resiliency inventory (MFRI)
Authors: Peterson Patricia , Morrison Bill , Laurie Robert , Bolaños Gramajo Viviane Yvette , Morrison John Brock 
Published: February 2020

PurposeThis paper explores the use of the mental fitness and resiliency inventory (MFRI) as a tool for the management of workplace health and well-being. The MFRI provides information on the extent to which positive workplace practices are experienced within three mental fitness domains and five resiliency domains. The purpose of this study was to investigate the factorial structure and internal consistency of the MFRI.Design/methodology/approachThe MFRI was administered to 1,519 employees in multiple workplace environments in Canada. The factorial structure of the MFRI was examined to conduct confirmatory factor analysis (CFA). In addition to the CFA indexes, the internal consistency of each latent construct was calculated, with results reported using Cronbach's coefficient alpha.FindingsThe reliability of the MFRI is very high (alpha = 0.973). The fit indexes from the CFA indicate that the model is permissible. The MFRI can be used with confidence to highlight mental fitness and resiliency strengths, as well as areas needing further development in workplace environments.Research limitation/implicationsLimitations may include the selection of fit indexes upon which to base judgment as to whether the model is satisfactory. Although the MFRI model has been confirmed based on the data from the study sample, there is not yet sufficient data to conclude that the model is a true predictive model. Current and ongoing research will enable elaboration on this matter. In addition, formal documented observations regarding the MFRI's face validity and ease of explanation and understanding of the results may confirma prioriexpectations on the part of the users and may strengthen the conclusions from this study.Practical implicationsImplications for workplaces arising from the validation of the MFRI include a growth in capacity to measure the existence of positive psychology practices within organizational environments and to identify and address areas for needed growth and development. By assessing the prevalence of mental fitness and resiliency practices in workplace environments, reports can be produced that indicate various levels of development and integration of these practices. The application of the MFRI facilitates the use of evidence-informed decision-making in addressing organizational goals related to positive workplace cultures.Originality/valueThe MFRI is a new, validated instrument that measures the presence of positive practices that contribute to healthy and effective workplace cultures. The results of the MFRI provide workplace health managers with a profile of organizational strengths (practices that are embedded and comprehensive) and areas for development (practices requiring promotion and capacity building) related to mental fitness and resiliency.
Disentangling the factors driving electronic word-of-mouth use through a configurational approach
Authors: Wang Ping , Li Hongxiu , Liu Yong 
Published: February 2020

PurposeThe purpose of this paper is to show how different combinations of the subdimensions of electronic word-of-mouth (eWOM) information quality (consisting of its accuracy, completeness, relevance, timeliness, and sidedness) may affect consumers' eWOM use behavior from a configurational approach.Design/methodology/approachBased on a synthesis of past literature, five precursors of eWOM use were considered. A fuzzy-set qualitative comparative analysis (fsQCA) was employed to understand the configurations that lead to travel-related eWOM use. The study was conducted with 311 consumers from an online travel service company.FindingsFindings identified six solutions that explain eWOM use. EWOM accuracy, completeness, and sidedness are found to be core conditions reinforcing consumers' use of eWOM in combination with different peripheral conditions, including the subdimensions of eWOM information quality and the traits of the consumers (such as social media use time and gender).Practical implicationsFactors related to eWOM information quality and traits of the consumers, when considered in combination, predict eWOM use behavior in particular sets of conditions.Originality/valueThis work enriches the eWOM literature by providing an in-depth understanding of eWOM use from a configuration perspective. Configuration analysis serves as a better tool for explaining the complex relationships among variables than a regression analysis approach does. Additionally, in response to the need to move beyond multiple regression analysis to algorithmic approaches, this study shifts the emphasis from a symmetric paradigm to an asymmetric perspective for data analysis focused on eWOM use.
Cooperative advantage during the pioneer movement west
Authors: Lloyd Robert , Mertens Daniel , Adams Ashley , Pruden Christianna , Bates Angela 
Published: February 2020

Purpose
The purpose of this paper is to establish a supported and validated reference point for understanding how Nicodemus, Kansas warrants significant inclusion in both the history of management and current entrepreneurship education.

Design/methodology/approach
This paper consists of a literature review to identify the salient historical and theoretical importance of the community of Nicodemus, Kansas. The research was conducted by reviewing and couching the research in this context.

Findings
Nicodemus, born out of an entrepreneurial spirit of newly liberated Black Americans, is the surviving entrepreneurial force for the African-American efforts in the western US expansion efforts in the later 1800s. The community, courage and cooperative views of the settlers were instrumental in overcoming a variety of hardships inherent in the location, society and time period to not only survive but also deliver growth and success. Nicodemus personified cultural pride and self-reliance, which fueled personal and commercial success.

Practical implications
The cooperative advantage is justified to be included in discussions of American management history, taught in the entrepreneurship curriculum and used by practitioners.

Social implications
Collective courage and cooperative advantage used by Nicodemus carries implications for how modern Black communities can advance their economic and social agendas.

Originality/value
Coverage of Black contributions management and entrepreneurship is scant, but a Black Enlightenment period has recently changed that scholars have recently begun to cover these significant moments in the literature (Prieto and Phipps, 2019). We argue that Nicodemus as an entrepreneurial community serves as a case study that needs contextualization in this Black Enlightenment era and holds pertinent implications for modern Black communities.
Cost-driven motives to relocate manufacturing abroad among small- and medium-sized manufacturers
Authors: Stentoft Jan , Wickstrøm Kent Adsbøll , Haug Anders , Philipsen Kristian 
Published: February 2020

PurposeThe purpose of this paper is to advance the understanding of how Industry 4.0 related technologies affect the relocation of manufacturing abroad by small and medium-sized enterprises.Design/methodology/approachThis paper contains an empirical analysis of how Industry 4.0 related technologies affect the cost-driven relocation of manufacturing abroad based on 191 comprehensive and full responses to a questionnaire survey distributed in 2018 among small- and medium-sized Danish manufacturers.FindingsThis paper builds upon data, which reveals that companies' pursuit of cost-focused competitive strategies is positively correlated with relocating manufacturing abroad. However, the data also shows that the more Industry 4.0-ready decision-makers are, the less cost-focused strategy drives manufacturing abroad. Furthermore, perceived barriers to Industry 4.0 related technologies promote the cost-driven relocation of manufacturing abroad whereas perceived drivers decrease this phenomenon.Research limitations/implicationsThis paper is based on the answers given by a single respondent from each company and only on Danish respondents.Practical implicationsThe results indicate a need to invest resources to obtain a better knowledge of Industry 4.0 related technologies when used in processes involved in decisions about where to locate manufacturing.Originality/valueThis paper contains new, empirically founded information about how Industry 4.0 related technologies affect the cost-driven relocation of manufacturing abroad from the perspective of small- and medium-sized manufacturers.
Environmental and social impacts identification for small-scale alluvial mining projects
Authors: Famiyeh Samuel , Kwarteng Amoako , Darko Disraeli Asante , Osei Vivian 
Published: February 2020

PurposeThe purpose of the work is to use a systematic process to identify the environmental and social impacts of small-scale alluvial gold mining projects using data from Ghana.Design/methodology/approachIn this work, we used survey data collected from experts in the mining sector. This was followed by the use of a risk analysis approach to identify the significant and non-significant environmental and social impacts.FindingsSeven key impacts associated with typical alluvial mining operations were identified. The first two are the loss of vegetation and the issue of airborne diseases from dust as a result of vegetation losses during the clearing of vegetation in the block out area. The third and fourth issues were loss of vegetation and airborne diseases as a result of vegetation losses during the removal of overburden. The fifth, sixth and seventh, most significant issues identified were the pollution from smoke fumes from the processing machines; and wastewater from the washing process. The last issue of significance was the dust pollution from the transportation of the washed gravel back to the mined pit.Research limitations/implicationsOne main limitation is that the data for this study were collected from Ghana.Practical implicationsThe results indicate the need for proper and systematic measures to identify the environmental and social impacts of mining activities.Originality/valueThe work provides some insights into the strategies of identifying environmental and social impacts of mining activities. It is also one of the key works that systematically identify environmental and social impacts of small-scale alluvial gold projects.