bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Kinetics of Peroxynitrite on Insulin Nitration Damage
Authors: Zhang Congxiao , Sun Fusheng , Zhang Congjiang , Luo Yunjing 
Published: September 2020

Abstract
Background: Insulin is one of the most important versatile hormones that is central to regulating the energy and glucose metabolism in the body. There has been accumulating evidence supporting that diabetes was associated with peroxynitrite and protein nitration, and insulin nitration induced by peroxynitrite affected its biological activity. Methods: In this paper, the kinetics of insulin nitration by peroxynitrite in physiological conditions was studied by the stopped flow technique. Results: We determined the values of the reactive rate constants of peroxynitrite decomposition and peroxynitrite-induced tyrosine nitration in the presence of insulin. The activation energy of peroxynitrite decomposition and 3-nitrotyrosine yield in the presence of insulin is 48.8 kJ·mol−1 and 42.7 kJ·mol−1 respectively. Conclusions: It is inferred that the glutamate residue of insulin accelerated peroxynitrite decomposition and tyrosine nitration by reducing the activation energy of reactions. The results could be beneficial for exploring the molecular mechanism of diabetes and offering a new target for diabetes therapies.
Practice, Training Need of Disaster Preparedness and Associated Factors in Nursing Staffs Working at Emergency Department, Amahra Regional State Referral Hospitals, Ethiopia, 2020G.C.&nbsp;A Hospital Based Cross Sectional Study&nbsp;
Authors: Tilahun Lehulu , Dessu Birhanu , Zeleke Mulusew 
Published: September 2020

Abstract
Background: Globally disasters are increasing at alarming rate that leads giving training and making preparing frontline emergency department nurses an important issue. Although disasters are manageable and preventable they are still considered as a major public health issues. Methods: Hospital based cross sectional study used to come up with the findings. All emergency department working nurses from the region referral hospitals were assumed as study subjects. The disaster information on respondents was gathered with self-administered written questionnaires. Data entry and coding was made by using epidata software manager v4.6.0.2 and further exported to spss version 26 for further analysis. Result: In our study finding most participants were males 68(66.7%). In addition mean age of data respondents were 31.2±5.8. It is found that 25(24.5%) of participants have adequate practice on the contrary 75(75.5%) of respondents have inadequate practice. Also, 40(39.2%) respondents need training on first aid and treatment principles, 37(36.3%) need training on disaster preparedness and 31(30.4%) need training on basic principles of disaster response. In multivariate analysis variables with practice of disaster preparedness are training performed in a hospital set up {P value= 0.047, OR: 0.282, 95%CI: (0.081-0.985)} and simulation done in hospital {P value= 0.002, OR: 0.071, 95%CI: (0.055-0.530)} were significantly associated. Conclusions: Skill level of emergency department nurses with regard to disaster preparedness is inadequate so that training supported with drills & simulation and education are mandatory.
CAZyome and Sugar Transportome Unveil Singular Aspects of the Lignocellulolytic Enzyme System of Penicillium echinulatum 2HH
Authors: Lenz Alexandre Rafael , Balbinot Eduardo , Oliveira Nikael Souza de , Abreu Fernanda Pessi de , Silva Scheila de Avila e , Camassola Marli , Dillon Aldo José Pinheiro , Perez-Rueda Ernesto 
Published: September 2020

Abstract
The authors have withdrawn this preprint due to author disagreement.
The Change of Cervical Spine Alignment Along with BMI in Asymptomatic Population: A Preliminary Analysis
Authors: Shao Zhenxuan , Dai Gaole , Zhao Qingqian , Wang Ben , Wen Rui , Zhang Xiaolei , Wu Ai-Min , Wang xiang-yang 
Published: September 2020

Abstract
Study design: A cross-sectional studyObjective:Describe the difference in the cervical sagittal alignment between various BMI people.Summary of Background Data:Cervical sagittal imbalance is implicated in the development of various spinal disorders, and obesity was recognized as a global epidemic. But there were few studies focusing on the correlation between cervical sagittal alignment and body mass index (BMI). Methods: This study enrolled 140 asymptomatic volunteers from July 2016 to July 2019. Demographic data included gender, age and BMI, and radiographic parameters included chin–brow vertical angle (CBVA), occipital slope (OS), orbital tilt (OrT), orbital index (OI), occiput-C2 lordosis (O-C2), cervical lordosis (CL), C2-C7 sagittal vertical axis (C2-C7SVA) , cranial tilting (CrT), cervical tilting (CeT), T1 slope (TS), neck tilt (NT), and thoracic inlet angle (TIA). The data were analyzed by ANOVA statistical analyses.Results: In terms of the occipitocervical parameters, we found that there was no significant difference (OS, P=0.970; OrT, P=0.970; OI, P=0.798); In terms of cervical parameters, we found significant correlations between BMI and parameters (C2-C7SVA, P=0.005; TZC2-C7, P=0.030; CrT, P= 0.050; CeT, P=0.013), which reflected that forward head posture increases as BMI rises; In terms of thoracic parameters, we also found significant correlations between BMI and parameters (NT, P=0.014; TIA, P=0.050), which reflected that thoracic inlet lifts as BMI rises.Conclusions: We found that there are the correlations between cervical sagittal alignment (C2-C7SVA, TZC2-C7, CL, CrT, and CeT) and BMI, and forward head posture increases and thoracic inlet lifts in the obese, which can provide clinical advice and remind surgeons of BMI effect in reconstructive surgery for better prognoses.
Field Trial Assessment of Two Commercial Vaccines Against Mycoplasma Hyopneumoniae
Authors: Marcos Marcial , Rabanal Javier , Pico David del , Bollo Jesús , Romero Alfredo , Pérez-Breña Maria Luisa , Jiménez Marta , Menjón Rut , Tejedor Teresa , Swam Hanny 
Published: September 2020

Abstract
Background: Mycoplasma hyopneumoniae (M.hyo) is one of the most prevalent pathogens causing respiratory disorders in swine populations as well as primary agent of porcine respiratory disease complex (PRDC). The aim of this trial is to compare the efficacy of two commercial vaccines under field conditions, applied via different routes of administration, as a tool to control disease.Results: 3 groups each consisting of 440 animals, were included in this longitudinal study; one group vaccinated against M.hyo with an intradermal vaccine (group I), a second group vaccinated with an intramuscular vaccine 2940 strain (group H) and finally a third group, not vaccinated as a control (group N). All animals were individually weighed at weaning; upon entry into the fattening unit and just prior to movement to the slaughterhouse. Pigs from each group were periodically blood sampled to evaluate the immunological status against other pathogens involved in PRDC. Tracheobronchial swabs were collected from 30 pigs per treatment group during the final weighing. Slaughterhouse lung lesion evaluations were developed to assess levels of enzootic pneumonia-like lesions (EP) and pleuritis (P).Statistical evaluation of all test parameters indicated a better performance of the intradermal vaccinated group (group I):· in terms of weight (kg) just prior to movement to the slaughterhouse, (I= 97.45; H = 93.44; N = 93.05).· The number of positive tracheobronchial swabs, as well as M.hyo load detected individually (I = 36.7% positive samples with log10 Ct average value 1.48; H = 100% with log10 Ct 1.40; and N = 96.7% with log10 Ct 1.40),· EP prevalence (I = 35.1%, H = 47.0%, N = 50.5%) and· Pleurisy prevalence (I = 22.4%, H = 29.7%, N = 39.3%).In terms of the involvement of other PRDC pathogens, the serological study could lead us to think that the agents studied were present at same level in the all 3 vaccination groups.Conclusion: In this study, intradermal vaccination provided better performance than unvaccinated control group and intramuscular vaccine, in terms of weight increase (versus intramuscular group); reduction of enzootic pneumonia and pleurisy lesions, and tracheobronchial M.hyo prevalence at the end of the finishing period.
WITHDRAWN: Expression and Prognosis Analysis of JMJD5&nbsp;in Human Cancers
Authors: Li Hui , Li Qun , Jing Hong , Zhao Jianghai , Zhang Hui , Ma Xuhui , Wei Lunshou , Dai Rujiang , Sun Weihong , Suo Zhimin 
Published: September 2020

Abstract
JumonjiC (JmjC) domain-containing protein 5 (JMJD5) plays an important role in cancer metabolism. However, the prognostic value of JMJD5 in most human cancers is still unknown. In this study, we aim to investigate the expression and prognostic value of JMJD5, immune cells infiltration, and the correlations among them. We found: The expression of JMJD5 was significantly lower in human breast carcinoma (BRCA), cholangiocarcinoma (CHOL), liver hepatocellular carcinoma (LIHC) and lung cancer (LUC) but higher in prostate adenocarcinoma (PRAD) and stomach adenocarcinoma (STAD) comparing to their respective normal tissues by online Tumor Immune Estimation Resource (TIMER) and immunohistochemistry (IHC) of tissue microarray sections (TMAs) respectively. And high expression of JMJD5 has better prognosis only in BRCA, LIHC, LUC but the opposite effect in STAD by the Kaplan-Meier Plotter databases analyses. Further analysis revealed JMJD5 expression is significant correlation with the abundance of six immune cells infiltration in above four cancers by TIMER, and evaluated the prognostic value by the combination of JMJD5 expression with either B cells or macrophages tumor infiltration by the COX proportional hazards model. Both the BRCA or lung adenocarcinoma (LUAD) patients with abundance of B cell and the STAD patients with low level of macrophage have a better cumulative survival by TIMER. In conclusion, we provided novel evidence of JMJD5 as an essential prognostic biomarker in cancers through analyses the correction of the JMJD5 expression, tumor-infiltrating B cells and macrophages and prognostic value. This study offers new perspectives therapeutic target in BRCA, LUAD and STAD.
Spike mutation D614G alters SARS-CoV-2 fitness and neutralization susceptibility
Authors: Shi Pei-Yong , Plante Jessica , Liu Yang , Liu Jianying , Xia Hongjie , Johnson Bryan , Lokugamage Kumari , Zhang Xianwen , Muruato Antonio , Zou Jing , Fontes-Garfias Camila , Mirchandani Divya , Scharton Dionna , Kalveram Birte , Bilello John , Ku Zhiqiang , An Zhiqiang , Freiberg Alexander , Menachery Vineet , Xie Xuping , Plante Kenneth , Weaver Scott 
Published: September 2020

Abstract
A spike protein mutation D614G became dominant in SARS-CoV-2 during the COVID-19 pandemic. However, the mutational impact on viral spread and vaccine efficacy remains to be defined. Here we engineer the D614G mutation in the SARS-CoV-2 USA-WA1/2020 strain and characterize its effect on viral replication, pathogenesis, and antibody neutralization. The D614G mutation significantly enhances SARS-CoV-2 replication on human lung epithelial cells and primary human airway tissues, through an improved infectivity of virions with the spike receptor-binding domain in an “up” conformation for binding to ACE2 receptor. Hamsters infected with D614 or G614 variants developed similar levels of weight loss. However, the G614 virus produced higher infectious titers in the nasal washes and trachea, but not lungs, than the D614 virus. The hamster results confirm clinical evidence that the D614G mutation enhances viral loads in the upper respiratory tract of COVID-19 patients and may increases transmission. For antibody neutralization, sera from D614 virus-infected hamsters consistently exhibit higher neutralization titers against G614 virus than those against D614 virus, indicating that (i) the mutation may not reduce the ability of vaccines in clinical trials to protect against COVID-19 and (ii) therapeutic antibodies should be tested against the circulating G614 virus before clinical development.
Integration Analysis of Differential Expressed Genes Regulated by Differential Methylated Regions for Predicting Prognosis in Human Colon Cancer
Authors: Peng Xiaowen , Dong Dong , Ou Wentao , Xie Yongke , Luo Yuqi 
Published: September 2020

Abstract
Background Colon cancer is a leading cause of cancer-associated death globally, and numerous evidences show that different expressed gens (DEGs) regulated by differential methylated regions (DMRs) act an important role in tumor biology. However, the specific regulatory mechanism of DEGs related to DERs in colonic carcinogenesis is still unclear.Materials and methods RNA sequencing data and DNA methylation data of 455 colon adenocarcinoma (COAD) cases and 41 normal controls were downloaded from The Cancer Genomic Atlas (TCGA) to investigate the significant DEGs and DMRs. Gene ontology (GO) functional enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed by DAVID database. To identify the hub genes regulated by methylation, univariate cox and multivariate cox regression analyses were concluded. Furthermore, Riskscore and nomogram were built to identify the prognosis prediction power of the hub genes in colon cancer patients. Results A total of 133 DEGs regulated by DMRs were identified through analyzing RNA-seq data and DNA methylation data from TCGA; GO functional enrichment and KEGG pathway enrichment analysis showed that the genes involved in the initiation and progression of colon cancer. Univariate cox regression analysis and multivariate cox regression analysis focused on the 7 hub genes associated with overall survival, whose expression negatively correlated with their methylated level; Riskscore and nomogram model showed that the hub gens served as potential biomarker for the prognosis prediction of colon cancer patient. Conclusion Our funding suggests that the DEGs regulated by DMRs involve in the carcinogenesis and development of colon cancer, and the aberrant methylated DEGs associated with overall survival of patients may be potential diagnosis and therapeutic targets for colon cancer.
The Volatile and Heterogenous Gut Microbiota Shifts of COVID-19 Patients Over The Course of A Probiotics-Assisted Therapy
Authors: Wu Chunyan , Xu Qian , Cao Zhan , Pan Dengdeng , Zhu Ying , Wang Sheng , Liu Danping , Song Zhigang , Jiang Wei , Ruan Yumeng , Qin Nan , Lu Hongzhou , Qin Huanlong 
Published: September 2020

Abstract
Background Coronavirus disease 2019 (COVID-19) has rapidly become a global pandemic, and little is known regarding the gut microbiota dynamics of the disease that often features a drastic and swift progression. Here we employed analyses of 16S rRNA gene sequencing and metatranscriptome to investigate the gut microbiome characteristics of a group of COVID-19 patients over the course of a probiotics-assisted therapy. Results The COVID-19 patients exhibited apparent microbiota alterations characterized by prominent compositional and functional shifts, which included taxonomic changes (e.g., increased relative abundance of Enterococcus and Rhodococcus, and decreased relative abundance of Faecalibacterium and Clostridium XlVa) and transcriptional changes (e.g., increased transcriptional activities of Escherichia coli and Klebsiella pneumoniae, virulence factors, and antibiotic resistance genes, and decreased activities of Faecalibacterium prausnitzii). Importantly, there were great interpersonal heterogeneity and intertimepoint fluctuations, as the most abundant or transcriptionally active taxa often greatly differed among individual patients and timepoints. Coincided with the resolution of respiratory symptoms, after the therapy some patients showed signs of recovery in the gut microbiome abnormalities. Associations were identified between gut and airway taxa and serum factors. Conclusion Our findings suggested that there is a lack of gut microbiota stability in COVID-19 patients and that measures are needed to ameliorate the gut microbiome perturbations in the patients to improve the prognosis. In addition, inclusion of probiotics is safe for treating COVID-19 patients and may improve their prognosis. Trial registration ISRCTN, ChiCTR2000029999. Registered 19 February 2020, http://www.chictr.org.cn/showprojen.aspx?proj=49717
A Virtual Medical Faculty Development Program for Remote Teaching, Pilot for Replication
Authors: Hegazy Nagwa Nashaat , Soliman Saeed S.A , Ahmed Samar A , Ahmed Marwa M 
Published: September 2020

Abstract
Background: Due to COVID 19 pandemic, several universities have experienced a paradigm shift from classroom to online teaching/ learning. The digital transformation of learning management systems has become a necessity rather than a luxury. Especially baby boomers and generation x may struggle to cope with the challenging transformation; hence there is a dire need for a faculty development program to achieve the goal of that transformation and bridge the gaps in faculty’s technology skills and competencies. Objectives: The primary purpose of the virtual medical faculty development program (VFDP) is to equip educators with technology competencies shown to be conducive to remote online learning. Methodology: An interventional prospective study was held in a university setting. A need analysis was conducted to prioritize the critical technological skills of for faculty members. Based on this analysis, only the indispensable skills that participating faculty members were expected to acquire were included in the program. From each department, 3- 4 Faculty participants were recruited to join in the program with an overall 93 enrolled participants from 26 departments in the Medical School . The intervention comprised five sessions, a session every other day, and lasted for ten days. The Kirkpatrick model was utilized to evaluate the program. Results: Almost 81% of faculty members completed the program and 80 % of participants were satisfied with the content of the program. There was a statistically significant difference between the perceived ability of the participants to share and record video lectures before and after the VFDP (p value <0.001). Conclusion: The virtual medical faculty development program (VFDP) has supported the participating faculty in developing their needed technological competencies required to bridge the gap of remote teaching/learning.
Intra-tumoral injection of caerin 1.1 and 1.9 peptides in vaccinated TC-1 tumour bearing mice with PD-1 blockade modulates macrophage heterogeneity and the activation of CD8+ T cells in the tumour microenvironment
Authors: Ni Guoying , Wu Xiaolian , Liu Ying , Li Hejie , Chen Shu , Fogarty Conor , Frazer Ian , Chen Guoqiang , Liu Xiaosong , Wang Tianfang 
Published: September 2020

Abstract
Development of a vaccine formula that alters the tumour-infiltrating lymphocytes to be more immune active against a tumour is key to the improvement of clinical responses to immunotherapy. Here, we demonstrate that, in conjunction with E7 antigen specific immunotherapy, and IL-10 and PD-1 blockade, intra-tumoral administration of caerin 1.1 and 1.9 peptides further improves the tumour microenvironment (TME) when compared with injection of a control peptide. We used single cell transcriptomics and mass spectrometry-based proteomics to quantify changes in cellular activity across different cell types within the TME. We show that the injection of caerin 1.1/1.9 increases immune activating macrophages and NK cells, while reducing immunosuppressive macrophages with M2 phenotype, and increased numbers of activated CD8+ T cells with higher populations of memory and effector-memory CD8+ T subsets. Proteomic profiling demonstrated activation of Stat1 modulated apoptosis and production of nitric oxide. Further, computational integration of the proteome with the single cell transcriptome was consistent with deactivation of immune suppressive B cell function following caerin 1.1 and 1.9 treatment.
Study on Mine Pressure Control by Ground Fracturing High-level Hard Rock Strata
Authors: Gao Rui , Kuang Tiejun , Zhang Yanqun , Zhang Wenyang , Quan Chunyang 
Published: September 2020

Abstract
During extra-thick coal seam mining, the high-level thick and hard strata are the main reason for the presence of a strong ground pressure in the working face; however, there is no active and effective control technology for high-level hard strata. This paper proposes the concept of ground fracturing hard roofs, and the physical simulation was used to study the control effect of ground fracturing on the strata breaking structure and energy release. The results showed that the ground fracturing changed the structural characteristics of the strata and reduced the energy release intensity and overburden movement spatial extent, which had a significant control effect on the ground pressure. The Datong mining area was selected as the engineering background, a ground horizontal well fracturing engineering test was conducted on site, and a 20-m-thick hard rock layer, which was 110 m vertically away from the coal seam, was determined as the fracturing target layer. On-site microseismic monitoring showed that the crack propagation length was up to 216 m, and the height was up to 50 m. On-site mine pressure monitoring showed that the roadway deformation was reduced to 100 mm, the periodic weighting characteristics of the hydraulic supports were not obvious, the ground pressure in the working face was significantly controlled, and the ground fracturing was successful. Ground fracturing changed the occurrence characteristics of the high-level hard strata, which is beneficial to ameliorate the stress environment of a working face and provide a new approach of hard rock control.
The rationale for the modes of bonding veneer in the manufacture of plywood, manufactured with the use of melaminecarbamideformaldehyde resins
Authors: Соколова Е.Г. 
Published: September 2020

Повышение эффективности производства фанеры, её конкурентоспособности в ряду плитных материалов является актуальной проблемой. Основными задачами являются повышение производительности труда, снижение токсичности продукции, её материало-, энерго- и трудоёмкости. Поставленные задачи решаются путем применения новых смол, составов клеев, технологических подходов, в частности с помощью применения меламинокарбамидноформальдегидных смол, модифицированных аэросилом техническим. Выполненные исследования позволили обосновать режимы склеивания пакетов шпона разной толщины с применением модифицированного клея на основе меламинокарбамидоформальдегидной смолы. За счёт уменьшения времени склеивания снижена продолжительность выдержки при максимальном давлении и снижении давления на втором этапе прессования с целью уменьшения упрессовки. Установлены необходимая продолжительность выдержки при максимальном давлении и давление на втором этапе прессования, которые обеспечивают необходимую прочность и минимальную упрессовку пакетов при изготовлении фанеры. Представлены режимы склеивания пакетов шпона разной толщины, значения упрессовки и прочности фанеры.
Improving the efficiency of production of plywood, its a competitive industry in a number of panel materials is an important issue. The main objectives are improving performance, reducing toxicity of the product, its material, energy and labor cost. The tasks are solved by the use of new resins, adhesives formulations, technological approaches, in particular through the use of melaminecarbamideformaldehyde resins, modified aèrosilom technical. Studies have allowed to justify the modes of gluing of the veneer packs of different thickness with the application of the modified adhesive based on melaminecarbamideformaldehyde resin. By reducing pressing time were decreased exposure at maximum pressure and the pressure reduction in the second stage of compression to reduce thinning of plywood. Set the required time of exposure at maximum pressure and the pressure in the second stage of pressing, which provide the necessary strength and minimum thinning of plywood. Describes the modes of gluing of the packages of veneers of different thickness, the values of thinning of plywood.