bluuebunny/crossref_metadata_2025_split huggingface dataset:
Correlated fluorescence microscopy and multi-ion beam secondary ion mass spectrometry imaging reveals phosphatidylethanolamine increases in the membrane of cancer cells over-expressing the molecular chaperone subunit CCTδ
Authors: Fletcher John S. , Sämfors Sanna , Vallin Josefine , Svanström Andreas , Grantham Julie 
Published: October 2020

AbstractChanges in the membrane composition of sub-populations of cells can influence different properties with importance to tumour growth, metastasis and treatment efficacy. In this study, we use correlated fluorescence microscopy and ToF-SIMS with C60+ and (CO2)6k+ ion beams to identify and characterise sub-populations of cells based on successful transfection leading to over-expression of CCTδ, a component of the multi-subunit molecular chaperone named chaperonin-containing tailless complex polypeptide 1 (CCT). CCT has been linked to increased cell growth and proliferation and is known to affect cell morphology but corresponding changes in lipid composition of the membrane have not been measured until now. Multivariate analysis of the surface mass spectra from single cells, focused on the intact lipid ions, indicates an enrichment of phosphatidylethanolamine species in the transfected cells. While the lipid changes in this case are driven by the structural changes in the protein cytoskeleton, the consequence of phosphatidylethanolamine enrichment may have additional implications in cancer such as increased membrane fluidity, increased motility and an ability to adapt to a depletion of unsaturated lipids during cancer cell proliferation. This study demonstrates a successful fluorescence microscopy-guided cell by cell membrane lipid analysis with broad application to biological investigation.Graphical abstract
CD1d expression in glioblastoma is a promising target for NKT cell-based cancer immunotherapy
Authors: Hara Ayaka , Koyama-Nasu Ryo , Takami Mariko , Toyoda Takahide , Aoki Takahiro , Ihara Fumie , Kobayashi Masayoshi , Hirono Seiichiro , Matsutani Tomoo , Nakayama Toshinori , Iwadate Yasuo , Motohashi Shinichiro 
Published: October 2020

AbstractGlioblastoma is the most common and aggressive type of brain tumor with high recurrence and fatality rates. Although various therapeutic strategies have been explored, there is currently no effective treatment for glioblastoma. Recently, the number of immunotherapeutic strategies has been tested for malignant brain tumors. Invariant natural killer T (iNKT) cells play an important role in anti-tumor immunity. To address if iNKT cells can target glioblastoma to exert anti-tumor activity, we assessed the expression of CD1d, an antigen-presenting molecule for iNKT cells, on glioblastoma cells. Glioblastoma cells from 10 of 15 patients expressed CD1d, and CD1d-positive glioblastoma cells pulsed with glycolipid ligand induced iNKT cell-mediated cytotoxicity in vitro. Although CD1d expression was low on glioblastoma stem-like cells, retinoic acid, which is the most common differentiating agent, upregulated CD1d expression in these cells and induced iNKT cell-mediated cytotoxicity. Moreover, intracranial administration of human iNKT cells induced tumor regression of CD1d-positive glioblastoma in orthotopic xenografts in NOD/Shi-scid IL-2RγKO (NOG) mice. Thus, CD1d expression represents a novel target for NKT cell-based immunotherapy for glioblastoma patients.
Genetic association study of fatal pulmonary embolism
Authors: Meißner Lisa , Schürmann Peter , Dörk Thilo , Hagemeier Lars , Klintschar Michael 
Published: October 2020

AbstractPulmonary embolism (PE) is a complex multi-factorial disease and represents one manifestation of venous thromboembolism (VTE). Most commonly PE constitutes a complication of VTE’s other clinical presentation deep vein thrombosis (DVT). The majority of studies concerning risk factors do not distinguish between PE and DVT. The risk factors are often estimated to be alike, but the prevalence and the risk associated with the major genetic factor Factor V Leiden differ between the two disease states. We have investigated the association of 22 SNPs with PE in 185 PE case and 375 healthy control subjects. At p = 0.05, eight SNPs presented with nominally significant evidence of association (EOA), although no significantly different genotype distributions remained between cases and controls after Bonferroni correction. Three of these variants (rs1800790, rs3813948, rs6025) showed EOA in the main analysis, and five variants (rs169713, rs1801131, rs4524, rs5985 and rs8176592) demonstrated EOAs in subgroups. Genomic variation modulating Factor V, Factor XIII, Beta fibrinogen (FGB), TFPI or HIVEP1 should be worth to be followed in subsequent studies. The findings of this study support the view that PE represents a complex disease with many factors contributing relatively small effects. Larger sample sizes will be required to reliably detect these small effects.
Solenoidal extensions in domains with obstacles: explicit bounds and applications to Navier–Stokes equations
Authors: Fragalà Ilaria , Gazzola Filippo , Sperone Gianmarco 
Published: October 2020

AbstractWe introduce a new method for constructing solenoidal extensions of fairly general boundary data in (2d or 3d) cubes that contain an obstacle. This method allows us to provide explicit bounds for the Dirichlet norm of the extensions. It runs as follows: by inverting the trace operator, we first determine suitable extensions, not necessarily solenoidal, of the data; then we analyze the Bogovskii problem with the resulting divergence to obtain a solenoidal extension; finally, by solving a variational problem involving the infinity-Laplacian and using ad hoc cutoff functions, we find explicit bounds in terms of the geometric parameters of the obstacle. The natural applications of our results lie in the analysis of inflow–outflow problems, in which an explicit bound on the inflow velocity is needed to estimate the threshold for uniqueness in the stationary Navier–Stokes equations and, in case of symmetry, the stability of the obstacle immersed in the fluid flow.
Natural blue zircon from Vesuvius
Authors: Sun Yi , Schmitt Axel K. , Häger Tobias , Schneider Marcus , Pappalardo Lucia , Russo Massimo 
Published: October 2020

AbstractZircon from syenitic ejecta of Vesuvius (Campania, Italy) is unusually blue, a property shared with gem zircon from Ratanakiri province (Cambodia), which turns from natural reddish-brown to blue when heated under reducing conditions. Here, the origins of these unusual crystals were traced through geochronology, trace elements, and O-Hf isotopic compositions. The causes of its colour were investigated through optical and electron microscopy, optical absorption spectroscopy, and Raman microspectroscopy. Colour stability upon heating and ultraviolet light (UV) exposure was tested using Ratanakiri zircon as a control. Vesuvius zircon contains vesiculated zones with abundant inclusions ~2.5 μm to <100 nm in diameter (mostly U-rich thorianite and pyrochlore-group minerals), while homogeneous zircon domains are high in Th and U (up to 5.9 and 1.8 wt%, respectively). Its blue colouration is stable under UV radiation, as well as heat-treatment under reducing conditions (1000 °C; >15 h). Turbid domains rich in large inclusions change to yellow-brown after heating under oxidizing conditions, while transparent domains remain pale blue or colourless. Optical absorption spectra display sharp absorption lines attributed to U4+, and slightly elevated absorption towards shorter wavelengths. The ~1007 cm−1 ν3(SiO4) Raman band is broadened due to lattice distortion by non-stoichiometric elements in high-Th/-U zircon, whereas narrow bands in inclusion-rich domains indicate a decrease in lattice strain due to inclusion precipitation. Blue colouration in Vesuvius zircon is explained by the effect of light scattering (Rayleigh and/or Mie scattering) on highly refractive actinide-rich inclusions ranging in size from <1/10 to few multiples of the wavelengths of visible light. Inclusions likely formed during fluid-mediated coupled dissolution-reprecipitation that locally transformed lattice-strained actinide-rich zircon within several hundreds of years prior to eruption.
Comparison of two curing protocols during adhesive cementation: can the step luting technique supersede the traditional one?
Authors: Tosco Vincenzo , Monterubbianesi Riccardo , Orilisi Giulia , Sabbatini Simona , Conti Carla , Özcan Mutlu , Putignano Angelo , Orsini Giovanna 
Published: October 2020

AbstractThis study aims to compare the degree of conversion of two different curing protocols used during adhesive cementation. The following resin luting agents were tested: Hri Flow (MF) and pre-heated Hri Micerium (MH); light-cure Nexus Third Generation (NX3L) and dual-cure Nexus Third Generation (NX3D); dual cured RelyX Ultimate (RXU) and light-cure RelyX Veneers (RXL). For each tested material, ten samples were prepared and divided into two groups which had different curing protocols (P1 and P2): in P1, samples were cured for 40 s; in P2, samples were cured for 5 s, and then, after 20 s, cured again for additional 40 s. The degree of conversion (DC) was evaluated both during the first 5 min of the curing phase and after 1, 2, 7, 14 and 28 days (p = 0.05). Different trends were observed in DC values after 5 min by comparing P1 and P2. In both P1 and P2, DC decreased as follows, MH > MF > NX3L > RXL > RXU > NX3D. There were significant differences of DC values among all resin luting agents (p < 0.05) in P1, while no significant differences existed between MH and MF, and NX3L and RXL in P2. At 1, 2, 7, 14 and 28 days the light curing luting agents had a higher DC than the dual luting agents (p < 0.05). P1 and P2 were not statistically different at each time point (p > 0.05). Both P1 and P2 protocols let achieve an acceptable DC after 28 days. The tested P2 can be safely used to lute indirect restorations, simplifying the removal of cement excesses.
One out of ten: low sampling efficiency of cloth dragging challenges abundance estimates of questing ticks
Authors: Nyrhilä Siiri , Sormunen Jani J. , Mäkelä Satu , Sippola Ella , Vesterinen Eero J. , Klemola Tero 
Published: October 2020

AbstractHard ticks (Acari: Ixodidae) act as important vectors of zoonotic pathogens. For instance, Borrelia burgdorferi s.l. spirochetes pose a severe health risk as aetiological agents of Lyme borreliosis. Commonly, to study the abundance of questing (host-seeking) ticks, a 1 m2 piece of cloth is dragged over vegetation for a determined distance. Here, we designed a tick-sampling study to estimate the sampling efficiency of this standard method. We established 10 m dragging transects in a hemiboreal mixed forest patch in SW Finland for a 5-day monitoring period. Five of the transects were cloth-dragged 3× a day, whereas another five transects were dragged 6× a day in a manner that after each morning, midday and afternoon dragging, a second dragging was conducted on the same transect immediately. Captured Ixodes ricinus ticks were subsequently analysed for tick-borne pathogens. The initial population size of nymphal ticks on a transect was approximated by the accumulated nymph catch from the dragging sessions. The sampling efficiency of the cloth dragging was low, as a single dragging in a previously untouched vegetation strip always caught less than 12% (mean 6%) of the estimated population of active nymphs that were assumed to be questing during the study. Clear results were not found for daily activity rhythm, as ticks were caught in all daily dragging sessions. Approximately every third nymph and every second adult carried a pathogen, but nothing indicated that the occurrence of a pathogen affected the likelihood of the tick being caught by cloth dragging. Our results suggest that only a minority of active ticks can be caught by a single cloth dragging. The abundance estimates in many tick investigations might thus be downward biased.
Associations Between Mode of Birth and Neuropsychological Development in Children Aged 4 Years: Results from a Birth Cohort Study
Authors: Takács Lea , Putnam Samuel P. , Monk Catherine , Dahlen Hannah G. , Thornton Charlene , Bartoš František , Topalidou Anastasia , Peters Lilian L. 
Published: October 2020

AbstractThe aim of this prospective longitudinal study was to examine the association between Cesarean section (CS) and child development and behavior. The sample consisted of 256 children who were born at term without serious perinatal pathologies. Their development and behavior was assessed at the age of four using Ages and Stages Questionnaire (ASQ-3), Children’s Behavior Questionnaire and Strength and Difficulties Questionnaire. Multivariate linear regression analyses were conducted to assess the association between CS and child outcomes. CS was associated with better scores in the Problem Solving domain of the ASQ in the whole sample. After stratifying by child sex, the positive association between CS and the Problem Solving domain was significant in boys, while no association was found in girls. Girls were rated less optimally in the Gross Motor domain of the ASQ when born via CS. Mode of birth was not associated with behavioral outcomes.
Intraoperative zero-heat-flux thermometry overestimates esophageal temperature by 0.26 °C: an observational study in 100 infants and young children
Authors: Nemeth Marcus , Lovric Marijana , Asendorf Thomas , Bräuer Anselm , Miller Clemens 
Published: October 2020

AbstractIn pediatric anesthesia, deviations from normothermia can lead to many complications, with infants and young children at the highest risk. A measurement method for core temperature must be clinically accurate, precise and should be minimally invasive. Zero-heat-flux (ZHF) temperature measurements have been evaluated in several studies in adults. We assessed the agreement between the 3M Bair Hugger™ temperature measurement sensor (TZHF) and esophageal temperature (TEso) in children up to and including 6 years undergoing surgery with general anesthesia. Data were recorded in 5 min-intervals. We investigated the accuracy of the ZHF sensor overall and in subgroups of different age, ASA classification, and temperature ranges by Bland–Altman comparisons of differences with multiple measurements. Change over time was assessed by a linear mixed model regression. Data were collected in 100 children with a median (1st–3rd quartile) age of 1.7 (1–3.9) years resulting in 1254 data pairs. Compared to TEso (range from 35.3 to 39.3 °C; median 37.2 °C), TZHF resulted in a mean bias of +0.26 °C (95% confidence interval +0.22 to +0.29 °C; 95% limits of agreement −0.11 to +0.62 °C). Lin’s concordance correlation coefficient was 0.89. There was no significant or relevant change of temperature over time (0.006 °C per hour measurement interval, p = 0.199) and no relevant differences in the subgroups. Due to the mean bias of +0.26 °C in TZHF, the risk of hypothermia may be underestimated, while the risk of hyperthermia may be overestimated. Nevertheless, because of its high precision, we consider ZHF valuable for intraoperative temperature monitoring in children and infants.
How Does Adolescents’ Openness to Diversity Change Over Time? The Role of Majority-Minority Friendship, Friends’ Views, and Classroom Social Context
Authors: Bayram Özdemir Sevgi , Özdemir Metin , Boersma Katja 
Published: October 2020

AbstractYoung people are growing up in increasingly “super-diverse” societies, and show variations in how they approach diversity and embrace differences. Developing a good understanding of why some youth appreciate and value diversity whereas others do not is crucial in identifying ways to promote social interactions among different groups in broader society. The current study examined whether adolescents follow different trajectories in their views on diversity, and identified possible factors behind how they change over time. The sample included 1362 adolescents residing in Sweden (Mage = 13.18, SD = 0.43, 48% girls). Adolescents reported on their openness to diversity and classroom social climate. The peer nominations method was used to measure majority-minority friendship, and friends’ views on diversity. Latent growth analysis showed that adolescents, on average, became more open to diversity over time, but with clear heterogeneity. Three distinct trajectories were identified as: high-increasing, average-increasing, and average-declining. Relative to the high-increasing group, the other two were more likely to be male and immigrant. Relative to the high-increasing group, adolescents on the average-increasing trajectory perceived their classroom climate as less cooperative, while the adolescents on the average-declining trajectory were less likely to have friends with positive views on diversity. The findings suggest that schools may serve as a shared ground for promoting openness to diversity.
Application of short-term methods to estimate the environmental stress cracking resistance of recycled HDPE
Authors: Gobetti Anna , Ramorino Giorgio 
Published: October 2020

AbstractEnvironmental stress cracking is a serious problem for polyethylene because it can cause failure without any visible warning due to the slow crack growth accelerated by aggressive agents. Tie molecules and entanglements are the main macromolecular characteristic increasing environmental stress cracking resistance, thus in this work mechanical and thermal properties governed by those macromolecular characteristics are determined by performing simple tests executable in the industrial laboratories for quality control on recycled high-density polyethylene. The mutual relation between the determined properties confirms their dependence on the investigated macromolecular characteristics and allows to predict in a comparative way the expected environmental stress cracking. The mechanical properties related to the environmental stress cracking resistance are the strain hardening modulus and the natural draw ratio. The strain hardening modulus is an intrinsic property that measure the disentanglement capability of the inter-lamellar links and the natural draw ratio is a highly sensitive parameter to the macromolecular network strength via the intercrystalline tie molecules. Since the measurement of these properties according to the standard ISO 18,488 requires a temperature chamber not often available in the industrial laboratories, the tensile test was performed also at room temperature and displacement rate 0.5 mm/min; a proportionality between the data obtained at different test condition emerged. The thermal property related to the environmental stress cracking resistance is the stepwise isothermal segregation ratio that state the chain fraction that generates a high rate of tie molecules responsible of environmental stress cracking resistance.
Big data analytics (BDA) and degree of internationalization: the interplay between governance of BDA infrastructure and BDA capabilities
Authors: Bertello Alberto , Ferraris Alberto , Bresciani Stefano , De Bernardi Paola 
Published: October 2020

AbstractIn order to face the challenges of internationalization and to cope more efficiently with the uncertainty of foreign expansion, firms are called to analyze an increasing amount of real-time semi-structured and unstructured datasets. In this sense, big data analytics (BDA) can become strategic in stimulating the international growth of small and medium-sized enterprises (SMEs). However, the specific relationship between BDA and internationalization has been analyzed fragmentarily within the mainstream literature. With the purpose of shedding light on this relationship, the authors drew on resource-based view (RBV) and collected data through a questionnaire directed to CEOs of 266 SMEs, receiving 103 responses. A quantitative analysis based on an Ordinary Least Squares (OLS) regression showed that the relationship between governance of BDA infrastructure and the degree of internationalization (DOI) is not significant, while the direct effect of BDA capabilities as well as the interaction term between BDA infrastructure and BDA capabilities are positive and significant. This suggests that the governance of BDA per se is not enough for enhancing internationalization in SMEs. On the contrary, this article points out the relevance of developing specific BDA capabilities and the existence of a positive interplay between governance of BDA infrastructure and BDA capabilities that can exploit the new knowledge coming from BDA in SME international growth.
The Biological Clock: Age, Risk, and the Biopolitics of Reproductive Time
Authors: Yopo Díaz Martina 
Published: October 2020

AbstractThe present article explores the social and subjective dimensions of the biological clock and its implications for reproductive time through a qualitative study based on 40 life story interviews of women from Santiago de Chile. Although the narrative of the biological clock has become a prevalent frame for addressing reproductive time in the context of late childbearing, age-related infertility, and the use of assisted reproductive technologies, few studies engage in an in-depth analysis of the biological clock—its boundaries, dynamics, and the particular ways in which it shapes women’s views and experiences of reproductive time. The present article aims to advance current knowledge on the intersection of time, reproduction, and biopolitics by arguing that the biological clock regulates reproductive time by shaping the boundaries and dynamics of female fertility through the clock. By determining reproductive time as quantitative, standardised, linear, and irreversible and by outlining the passing of time through pressure, risk, and burden, the biological clock determines when it is possible and desirable to have children and regulates reproduction, gender, and the female life course. These findings highlight the importance of critically addressing the narrative of the biological clock and its implications for women’s views and experiences of reproductive time.
Vividness as a natural kind
Authors: Tooming Uku , Miyazono Kengo 
Published: October 2020

AbstractImaginings are often characterized in terms of vividness. However, there is little agreement in the philosophical literature as to what it amounts to and how to even investigate it. In this paper, we propose a natural kind methodology to study vividness and suggest treating it as a homeostatic property cluster with an underlying nature that explains the correlation of properties in that cluster. This approach relies on the empirical research on the vividness of mental imagery and contrasts with those accounts that treat vividness as an explanatory primitive and with those that attempt to provide a definition. We apply the natural kind methodology to make several substantive (but also provisional) claims about the vividness of mental imagery. First, we will argue that it forms a homeostatic property cluster, in that it is reliably correlated with, but not defined by, some properties, such as the level of detail, clarity, perception-likeness and intensity. In arguing for this claim, we also show how the cluster can be modified in the light of empirical research by complementing it with a correlation between vividness and familiarity. Second, we will argue that these correlations can be explained by an underlying property at the architectural level; i.e., the availability of stored sensory information for the elaboration of a mental image.