bluuebunny/crossref_metadata_2025_split huggingface dataset:
Relationships Between Chemical Defenses of Common Toad (Bufo bufo) Tadpoles and Bacterial Community Structure of their Natural Aquatic Habitat
Authors: Ujszegi János , Vajna Balázs , Móricz Ágnes M. , Krüzselyi Dániel , Korponai Kristóf , Krett Gergely , Hettyey Attila 
Published: May 2020

AbstractMany organisms synthesize secondary metabolites against natural enemies. However, to which environmental factors the production of these metabolites is adjusted to is poorly investigated in animals, especially so in vertebrates. Bufadienolides are steroidal compounds that are present in a wide range of plants and animals and, if present in large quantities, can provide protection against natural enemies, such as pathogens. In a correlative study involving 16 natural populations we investigated how variation in bufadienolide content of larval common toads (Bufo bufo) is associated with the bacterial community structure of their aquatic environment. We also evaluated pond size, macrovegetation cover, and the abundance of predators, conspecifics and other larval amphibians. We measured toxin content of tadpoles using HPLC-MS and determined the number of bufadienolide compounds (NBC) and the total quantity of bufadienolides (TBQ). AICc-based model selection revealed strong relationships of NBC and TBQ with bacterial community structure of the aquatic habitat as well as with the presence of conspecific tadpoles. The observed relationships may have arisen due to adaptation to local bacterial communities, phenotypic plasticity, differential biotransformation of toxin compounds by different bacterial communities, or a combination of these processes. Bacterial groups that contribute to among-population variation in toxin content remain to be pinpointed, but our study suggesting that toxin production may be influenced by the bacterial community of the environment represents an important step towards understanding the ecological and evolutionary processes leading to microbiota-mediated variation in skin toxin profiles of aquatic vertebrates.
The World Ends Here, the World Begins Here: Bronze Age Megalithic Monuments in Western Scotland
Authors: Higginbottom Gail 
Published: May 2020

AbstractThis paper presents a study of free-standing Bronze Age megalithic monuments across western Scotland: Argyll, Lochaber, Kintyre, and the isles of Mull, Coll and Tiree. The original project was designed to unearth the locational choices of their builders, the reasons for these choices, and what they reveal about the belief systems of these societies. Using statistical analyses and 2D and 3D GIS, it will be demonstrated that vision is the main force behind locational decisions. The GIS analyses revealed that the builders chose a particular horizon shape, defined by qualities of distance, direction and relative apparent height as viewed from the monument (altitude). Significantly, approximately half the sites have the same locational variables as all the sites considered on the isles of Coll and Tiree (labelled ‘classic sites’: Higginbottom et al. in J Archaeol Method Theory 22:584–645, 2015.10.1007/s10816-013-9182-7), while the other half are the topographical reverse (‘reverse sites’), where the major ‘astronomical show’ differs due to the topographical differences between the site types. It is relevant to note that landscapes that block views of particular major astronomical phenomena in the south are significantly more common at reverse sites than at classic sites. Specific results pertaining to individual areas will also be highlighted. It will be seen that the interplay between the astronomy and the topographical choices of the builders at each site highlights possible cosmological ideologies that can be observed and that were shared across western Scotland.
Content driving exposure and attention to tweets during local, high-impact weather events
Authors: Eachus Joshua D. , Keim Barry D. 
Published: May 2020

AbstractThe use of Twitter to disseminate weather information presents need for the analysis of what types of messages, and specifically warning messages, incur exposure and attention. Having this knowledge could increase exposure and attention to messages and perhaps increase retransmission through Twitter. Two models describe the cognitive processing of tweets and warnings. The extended parallel process model describes components of an effective warning message. Even in a tweet, ignoring one or both critical components of a warning—threat and efficacy—could inhibit a user from taking the correct protective action. The protective action decision model (PADM) describes risk perception and factors that enable or disable one from giving attention to a message. The PADM also helps to define impressions, retweets or likes as metrics of exposure or attention to a tweet. Tweets from three Twitter accounts within one television market during two high-impact weather events were examined. From an individual account, impressions, retweets and likes were collected to identify commonalities to tweets with much exposure and attention. Results indicate photographs and geographically specific messages were popular. Second, from two competing television weather accounts, warning tweet formats were compared to identify exposure and attention to each. Warning tweets providing threat and efficacy performed best. The purpose of this work is twofold. First is to identify local trends to compliment findings from studies with large sample sizes. Second is to apply existing theory on warning message content to Twitter. This approach should benefit communication strategies of key information nodes—local meteorologists—during high-impact weather events.
Development of a conceptual model and patient-reported outcome measures for assessing symptoms and functioning in patients with heart failure
Authors: Moshkovich Olga , Benjamin Katy , Hall Katie , Murphy Ryan , von Maltzahn Robyn , Gorsh Boris , Sikirica Vanja , Saini Rajnish , Sprecher Dennis 
Published: May 2020

Abstract
Purpose
Heart failure (HF) is a common condition that places considerable burden on patients. We aimed to develop a patient-reported outcome (PRO) measure to assess the symptoms and impacts of HF.

Methods
Phase 1: a targeted literature review, expert interviews, and concept elicitation (CE) interviews with patients with HF (n = 26) were used to develop a conceptual model of the core symptoms and impacts of HF. To capture these concepts, three new fit-for-purpose PRO questionnaires were constructed in accordance with US Food and Drug Administration PRO guidance. Phase 2: three ‘waves’ of cognitive interviews were conducted with patients with HF (n = 28) to validate and refine the questionnaires.

Results
Three key symptoms—shortness of breath, oedema, and fatigue—were identified across the literature review, expert interviews and CE interviews. Several additional symptoms, cognitive changes and impacts of HF were reported in the CE interviews and included in the conceptual model. A 10-item symptom questionnaire (Heart Failure-Daily Symptom Diary) was constructed; cognitive testing showed that the final PRO measure was easy to understand/complete and relevant to patients with HF, confirming content validity. Two HF impact questionnaires were developed (Assessing Dyspnoea’s Impact on Mobility and Sleep and Heart Failure-Functional Status Assessment), but required refinement to ensure patient understanding.

Conclusions
Patient input contributed to the development of a PRO instrument for assessing physical and cognitive symptoms important to patients with HF using novel measurement strategies. Inclusion of daily metrics offers differentiation from other qualified instruments and may provide clinical insight for improving lifestyles. Additionally, two draft PRO measures may, after further validation, be useful to assess the impacts of HF.
Capability of well-being: validation of the Hungarian version of the ICECAP-A and ICECAP-O questionnaires and population normative data
Authors: Baji Petra , Farkas Miklós , Dobos Ágota , Zrubka Zsombor , Gulácsi László , Brodszky Valentin , Rencz Fanni , Péntek Márta 
Published: May 2020

Abstract
Purpose
We aimed to develop and assess the psychometric characteristics of the Hungarian language version of two well-being capability measures, the ICEpop CAPability measure for Adults/Older people (ICECAP-A/-O), and to establish population norms.

Methods
A cross-sectional survey was performed involving a representative sample of the Hungarian population. Socio-demographic characteristics, the use and provision of informal care were recorded. The Minimum European Health Module (MEHM), EQ-5D-5L, WHO-5 well-being index, happiness and life satisfaction visual analogue scale (VAS), Satisfaction with Life Scale (SWLS) measures were applied alongside the ICECAP-A (age-group 18–64) and ICECAP-O (age-group 65+).

Results
Altogether 1568 and 453 individuals completed the ICECAP-A/-O questionnaires, respectively. Cronbach’s alpha was 0.86 for both measures (internal consistency). Subgroup analyses showed positive associations between ICECAP-A/-O scores and marital status, employment, income, health status (MEHM) and informal care use (construct validity). Pearson correlations were strong (r > 0.5; p < 0.01) between ICECAP-A/-O indexes and EQ-5D-5L, WHO-5, happiness and satisfaction VAS and SWLS scores (convergent validity). The age, education, and marital status were no longer significant in the multiple regression analysis. Test–retest average (SD) scores were 0.88 (0.11) and 0.89 (0.10) for the ICECAP-A, and equally 0.86 (0.09) for the ICECAP-O (reliability).

Conclusion
This is the first study to provide ICECAP-A/-O population norms. Also, it is the first to explore associations with WHO-5 well-being index which, alongside the MEHM measures, enable estimates from routinely collected international health statistics. The Hungarian ICECAP-A/-O proved to be valid and reliable measurement tools. Socio-demographic characteristics had minor or no impact on ICECAP-A/-O. Other influencing factors deserve further investigation in future research.
The political economy of differentiated integration: The case of common agricultural policy
Authors: Malang Thomas , Holzinger Katharina 
Published: May 2020

AbstractThe past and arguably the future of the European Union (EU) are characterized by Differentiated Integration (DI). Whereas a number of studies examine country variance in the realization of DI due to state-level characteristics, scholars have rarely addressed sector-specific differentiation. We select Common Agricultural Policy (CAP) for such an analysis – the policy domain with the largest budget, most contestation in the Council of Ministers, most redistribution, and most differentiated legal acts. Building on liberal intergovernmentalism, we develop a demand and supply model to explain the number of opt-outs a country realizes in CAP legislation. We hypothesize that the member states’ demand for differentiation is driven by agricultural lobbyism and by the political receptiveness of governments; the supply-side is driven by member states’ voting or bargaining power; and the realized differentiations are a consequence of the interaction of demand and supply. Using all differentiations in new CAP legal acts from 1993 to 2012, we test these hypotheses in a time-series cross-section design. We find that the domestic level of agricultural protectionism, conservative parties in government and voting power are robust predictors of the realization of differentiation in CAP. Our results support the general claim of liberal intergovernmentalism, that domestic societal and economic interests and political bargaining power shape the course of (differentiated) integration.
Fatigue Strength of 316 L Stainless Steel Manufactured by Selective Laser Melting
Authors: Hatami Sepehr , Ma Taoran , Vuoristo Taina , Bertilsson Jens , Lyckfeldt Ola 
Published: May 2020

AbstractIn this study, the fatigue strength of 316 L stainless steel manufactured by selective laser melting (SLM) is evaluated. The effect of powder layer thickness and postmachining is investigated. Specimens were produced with 30 and 50 µm layer thickness and tested under high cycle fatigue in as-printed and postmachined conditions. Examination of the specimens reveals that in the as-printed condition, fatigue strength suffers from high roughness and surface tensile residual stresses as well as defects such as pores and lack of fusion voids. After machining, the fatigue strength was improved due to lower surface roughness, presence of compressive residual stresses, and removal of surface porosity. The results show that increasing the layer thickness (within the range tested) has a minor negative impact on fatigue strength; however, it has a major positive impact on the productivity of the SLM process. In addition, it is clear that the impact of postmachining on fatigue is far greater than that of the layer thickness.
1H, 13C and 15N NMR chemical shift assignments of cAMP-regulated phosphoprotein-19 and -16 (ARPP-19 and ARPP-16)
Authors: Thapa Chandan J. , Haataja Tatu , Pentikäinen Ulla , Permi Perttu 
Published: May 2020

AbstractProtein Phosphatase 2A, PP2A, the principal Serine/threonine phosphatase, has major roles in broad range of signaling pathways that include regulation of cell cycle, cell proliferation and neuronal signaling. The loss of function of PP2A is linked with many human diseases, like cancer and neurodegenerative disorders. Protein phosphatase 2A (PP2A) functions as tumor suppressor and its tumor suppressor activity is inhibited by the overexpression of PP2A inhibitor proteins in most of the cancers. ARPP-19/ARPP-16 has been identified as one of the potential PP2A inhibitor proteins. Here, we report the resonance assignment of backbone 1H, 13C and 15N atoms of human ARPP-19 and ARPP-16 proteins. These chemical shift values can provide valuable information for the further study of the dynamics and interaction of ARPP-proteins to PP2A using NMR spectroscopy.
Survival of Brucella abortus S19 and other Brucella spp. in the presence of oxidative stress and within macrophages
Authors: Jacob Jens , Finke Antje , Mielke Martin 
Published: May 2020

AbstractThe evolutionary “success” of the genus Brucella depends on the ability to persist both in the environment as well as inside of even activated macrophages of the animal host. For that, the Brucellae produce catalase and superoxide dismutase to defend against oxidative stress. Since the deletion of the mglA gene in the B. abortus S19 vaccine strain resulted not only in an increased tolerance to H2O2 but also in the induction of cytokines in macrophages, we here investigated the effect of oxidative stress (Fe2+ and H2O2) on the survival of B. abortus S19 and the isogenic B. abortus S 19 ∆mglA 3.14 deletion mutant in comparison with B. neotomae 5K33, Brucella strain 83/13, and B. microti CCM4915. These Brucellae belong to different phylogenetic clades and show characteristic differences in the mgl-operon. From the various Brucellae tested, B. abortus S19 showed the highest susceptibility to oxidative stress and the lowest ability to survive inside of murine macrophages. B. abortus S19 ∆mglA 3.14 as well as B. neotomae, which also belongs to the classical core clade of Brucella and lacks the regulators of the mgl-operon, presented the highest degree of tolerance to H2O2 but not in the survival in macrophages. The latter was most pronounced in case of an infection with B. 83/13 and B. microti CCM4915. The various Brucellae investigated here demonstrate significant differences in tolerance against oxidative stress and different survival in murine macrophages, which, however, do not correlate directly.
Screening and characterisation of virus causing yellow leaf disease of Tephrosia in Ethiopia
Authors: Kumar Alok , Hanson Jean , Jones Chris S. , Assefa Yilikal , Mulatu Fikerte 
Published: May 2020

AbstractTephrosia, an important medicinal plant, and a potential livestock feed, was found to be affected by a leaf yellowing disease at the Ziway field site of the International Livestock Research Institute. A total of fifty samples from 300 plants were collected from twenty Tephrosia species in three consecutive planting seasons; 2015, 2016 and 2017. The samples were screened for viral infection by dot-blot assay with antiserum targetting eight viruses. RT-PCR of dot-blot positive samples using virus specific primers gave an amplification product of the expected size (867 bp) only for cucumber mosaic virus (CMV) in Tephrosia senna. The amplified products were sequenced; coat protein sequence (657 bp) extracted, and submitted to NCBI database (Tep-Et; KY041651). Sequence alignment and phylogenetic analyses indicated that the isolate, Tep-Et, shared maximum identity [88.8–97.5% nucleotide (nt) and 89.4–96.3% amino acid (aa)] with CMV belonging to members of subgroup-I. To our knowledge, this is the first report of molecular characterisation of a CMV isolate infecting a new host, T. senna in Ethiopia.
Measurement precision at the cut score in medical multiple choice exams: Theory matters
Authors: Lahner Felicitas-Maria , Schauber Stefan , Lörwald Andrea Carolin , Kropf Roger , Guttormsen Sissel , Fischer Martin R. , Huwendiek Sören 
Published: May 2020

Introduction In high-stakes assessment, the measurement precision of pass-fail decisions is of great importance. A concept for analyzing the measurement precision at the cut score is conditional reliability, which describes measurement precision for every score achieved in an exam. We compared conditional reliabilities in Classical Test Theory (CTT) and Item Response Theory (IRT) with a special focus on the cut score and potential factors influencing conditional reliability at the cut score.
Methods We analyzed 32 multiple-choice exams from three Swiss medical schools comparing conditional reliability at the cut score in IRT and CCT. Additionally, we analyzed potential influencing factors such as the range of examinees’ performance, year of study, and number of items using multiple regression.
Results In CTT, conditional reliability was highest for very low and very high scores, whereas examinees with medium scores showed low conditional reliabilities. In IRT, the maximum conditional reliability was in the middle of the scale. Therefore, conditional reliability at the cut score was significantly higher in IRT compared with CTT. It was influenced by the range of examinees’ performance and number of items. This influence was more pronounced in CTT.
Discussion We found that conditional reliability shows inverse distributions and conclusions regarding the measurement precision at the cut score depending on the theory used. As the use of IRT seems to be more appropriate for criterion-oriented standard setting in the framework of competency-based medical education, our findings might have practical implications for the design and quality assurance of medical education assessments.
Modeling Determinants of Low Birth Weight for Under Five-Children in Ethiopia
Authors: Bekalo Daniel Biftu , Nigussie Zelalem Mehari , Yimer Belay Birlie , Anteneh Zelalem Alamrew 
Published: May 2020

AbstractLow birth weight (LBW) is a major determinant of morbidity, mortality and disability in infancy and childhood and has a long-term impact on health outcomes in adult life. This study was aimed to model LBW using marginal and generalized linear mixed models as well as identify the potential risk factors of LBW in Ethiopia. Data was taken from the 2011 Ethiopian demographic and health survey, which is a nationally representative survey of children in the 0–59 month age groups. Two model families, generalized estimating equation and alternating logistic regression models from marginal model family, and generalized linear mixed model from cluster specific model family were used for the analysis. The result showed that 34.8% of children were born with LBW. Alternating logistic regression model was best fits the data for population-averaged effects of the given factors on birth weight than generalized estimating equation model. Generalized linear mixed model with two random intercepts was the best model to evaluate within and between regional heterogeneity of birth weight. Both the best-fitted models gave the same conclusion that sex, wealth status, age, antenatal care, marital status, vaccination, anemia and mother education level were the determinant factors of LBW. This study contributes to the understanding of the individual and collective effect of maternal, socio-economic and child related factors influencing infant birth weight in Ethiopia.
Impact of an automated hand hygiene monitoring system combined with a performance improvement intervention on hospital-acquired infections
Authors: Knepper Bryan C. , Miller Amber M. , Young Heather L. 
Published: May 2020

AbstractObjective:Hand hygiene adherence has been associated with reductions in nosocomial infection. We assessed the effect of improvements in electronically measured hand hygiene adherence on the incidence of hospital-acquired infections.Methods:This quasi-experimental study was conducted in a 555-bed urban safety-net level I trauma center. The preintervention period was January 2015 through June 2016. Baseline electronic hand hygiene data collection took place from April through June 2016. The intervention period was July 2016 through December 2017. An electronic hand hygiene system was installed in 4 locations in our hospital. Performance improvement strategies were implemented that included education, troubleshooting, data dissemination, and feedback. Adherence rates were tracked over time. Rates of hospital-acquired infections were evaluated in the intervention units and in control units selected for comparison. The intervention period was subdivided into the initial and subsequent 9-month periods and were compared to the baseline period.Results:Electronically measured hand hygiene rates improved significantly from baseline to intervention, from 47% 77% adherence. Rates >70% continued to be measured 18 months after the intervention. Interrupted time series analysis indicated a significant effect of hand hygiene on healthcare facility-onset Clostridioides difficile infection rates during the first 9 months of the intervention. This trend continued during the final 9 months of the intervention but was nonsignificant. No effects were observed for other hospital-acquired infection rates.Conclusions:Implementation of electronic hand hygiene monitoring and performance improvement interventions resulted in reductions in hospital-onset Clostridioides difficile infection rates.
Thinking through others’ emotions: Incorporating the role of emotional state inference in thinking through other minds
Authors: Smith Ryan , Lane Richard D. 
Published: May 2020

Abstract
The active inference framework offers an attractive starting point for understanding cultural cognition. Here, we argue that affective dynamics are essential to include when constructing this type of theory. We highlight ways in which interactions between emotional responses and the perception of those responses, both within and between individuals, can play central roles in both motivating and constraining sociocultural practices.
The multicultural mind as an epistemological test and extension for the thinking through other minds approach
Authors: Christopoulos George I. , Hong Ying-yi 
Published: May 2020

Abstract
The multicultural experience (i.e., multicultural individuals and cross-cultural experiences) offers the intriguing possibility for (i) an empirical examination of how free-energy principles explain dynamic cultural behaviors and pragmatic cultural phenomena and (ii) a challenging but decisive test of thinking through other minds (TTOM) predictions. We highlight that TTOM needs to treat individuals as active cultural agents instead of passive learners.