bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evidence of Duodenal Epithelial Barrier Impairment and Increased Pyroptosis in Patients With Functional Dyspepsia on Confocal Laser Endomicroscopy and “Ex Vivo” Mucosa Analysis
Authors: Nojkov Borko , Zhou Shi-Yi , Dolan Russell D. , Davis Elisabeth M. , Appelman Henry D. , Guo Xueyan , Jackson Kenya , Sturm Matthew B. , Wang Thomas D. , Owyang Chung , Liu Julia J. , Chey William D. 
Published: August 2020

INTRODUCTION:
Duodenal epithelial barrier impairment and immune activation may play a role in the pathogenesis of functional dyspepsia (FD). This study was aimed to evaluate the duodenal epithelium of patients with FD and healthy individuals for detectable microscopic structural abnormalities.

METHODS:
This is a prospective study using esophagogastroduodenoscopy enhanced with duodenal confocal laser endomicroscopy (CLE) and mucosal biopsies in patients with FD (n = 16) and healthy controls (n = 18). Blinded CLE images analysis evaluated the density of epithelial gaps (cell extrusion zones), a validated endoscopic measure of the intestinal barrier status. Analyses of the biopsied duodenal mucosa included standard histology, quantification of mucosal immune cells/cytokines, and immunohistochemistry for inflammatory epithelial cell death called pyroptosis. Transepithelial electrical resistance (TEER) was measured using Ussing chambers. Epithelial cell-to-cell adhesion proteins expression was assessed by real-time polymerase chain reaction.

RESULTS:
Patients with FD had significantly higher epithelial gap density on CLE in the distal duodenum than that of controls (P = 0.002). These mucosal abnormalities corresponded to significant changes in the duodenal biopsy samples of patients with FD, compared with controls, including impaired mucosal integrity by TEER (P = 0.009) and increased number of epithelial cells undergoing pyroptosis (P = 0.04). Reduced TEER inversely correlated with the severity of certain dyspeptic symptoms. Furthermore, patients with FD demonstrated altered duodenal expression of claudin-1 and interleukin-6. No differences in standard histology were found between the groups.

DISCUSSION:
This is the first report of duodenal CLE abnormalities in patients with FD, corroborated by biopsy findings of epithelial barrier impairment and increased cell death, implicating that duodenal barrier disruption is a pathogenesis factor in FD and introducing CLE a potential diagnostic biomarker in FD.
Reduced Dose of Beta-Alanine Is Sufficient to Maintain Performance in Repeated Sprints
Authors: Zandona Bruna A. , Ramos Renan A. , de Oliveira Cleyton dos Santos , McAnulty Steven R. , Ferreira Luis H.B. , Smolarek Andre C. , Enes Alysson A.N. , Urbinati Keith Mary de Souza Sato , Aragon Alan A. , Schoenfeld Brad J. , de Souza Junior Tácito P. 
Published: August 2020

Abstract
Zandona, BA, Ramos, RA, de Oliveira, CdS, McAnulty, SR, Ferreira, LHB, Smolarek, AC, Enes, AAN, Urbinati, KMdSS, Aragon, AA, Schoenfeld, BJ, and de Souza Junior, TP. Reduced Dose of Beta-Alanine Is Sufficient to Maintain Performance in Repeated Sprints. J Strength Cond Res 36(6): 1636–1642, 2022—Beta-alanine (BA) supplementation has been shown to be effective in improving physical performance by increasing carnosine concentration. However, it is still necessary to know the effect of a maintenance dose on performance. Thus, this study aimed to investigate the effects of a maintenance dose of BA supplementation on performance. Forty-four anaerobically trained men with 23.9 ± 3.8 years of age, 176.0 ± 0.05 cm height, 81.2 ± 7.5 kg body mass, and 15.5 ± 2.9% of body fat performed a cycle ergometer test consisting of 4 sprints of 30 s with 4 minutes of active recovery. The study comprised 3 phases: (a) presupplementation, (b) supplementation with 6.4 g·d−1 BA or placebo, and (c) postsupplementation with a maintenance dose of 1.2 g·d−1 of BA or interruption of supplementation. Data were analyzed using generalized estimated equations with a priori 0.05 level of significance. The placebo group and interruption group presented a lower power (7.28 ± 0.66 and 7.71 ± 0.42 W·kg−1 vs. 8.04 ± 0.84 and 9.25 ± 1.18 W·kg−1, respectively; p < 0.05) during the third sprint in postsupplementation, whereas the maintenance group maintained the required power (7.47 ± 1.03 vs. 8.74 ± 1.07 W·kg−1; p > 0.05). The placebo group also presented higher percentage of fatigue (44.5% ± 12.3 and 44.8% ± 7.7 vs. 37.6 ± 7.2%; p = 0.021) and higher subjective perception of exertion (8.92 ± 0.90 vs. 8.00 ± 1.60; p = 0.028). Therefore, the maintenance dose of 1.2 g·d−1 BA was effective in maintaining performance, whereas a reduction in performance was observed after supplementation interruption.
La forma como ley natural en Francis Bacon
Authors: Pachón Damián 
Published: August 2020

El trabajo de investigación busca esclarecer la Forma baconiana como “ley natural”. Se sostiene que los otros sentidos del concepto de Forma en Bacon, tales como diferencia verdadera, fuente de emanación o naturaleza naturante, forman parte de su eclecticismo, pero en estricto sentido, esas expresiones no dicen nada en torno a las leyes de las naturalezas simples o de los fenómenos físicos. En contraste, la Forma como ley no sólo permite comprender mejor la peculiar lectura de Bacon sobre la legalidad natural, sino que posibilita establecer nexos con la parte operativa. En la investigación también se escudriñan las relaciones de la ley natural baconiana con la teología y la ley jurídica.
Atypical variants of chronic inflammatory demyelinating polyneuropathy with benign course: a clinical observation for 8 patients without pathogenic therapy
Authors: Grishina D. A. , Suponeva N. A. , Rizvanova A. S. 
Published: August 2020

Introduction. Atypical variants of chronic inflammatory demyelinating polyneuropathy are marked by its clinical heterogeneity and variable disease course.Aim of the study. To describe clinical, anamnestic and neurophysiological features of patients with atypical variants of chronic inflammatory demyelinating polyneuropathy, characterized by benign disease course, minimal motor involvement and not required pathogenic therapy.Materials and methods. 8 patients (7 men (87 %) and 1 woman (13 %) at the age of 52–77 years) with atypical variants of chronic inflammatory demyelinating polyneuropathy were analyzed: 5 patients (62.5 %) with asymmetric variant – multifocal acquired demyelinating sensorimotor neuropathy and 3 patients (37.5 %) with sensory variant. All patients were observed at the Research Center of Neurology for the period of 2016– 2019. In each patient the proper clinical and laboratory evaluation was performed along with nerve conduction study and nerve ultrasound.Results. The disease duration at the time of first visit was 1–8 years. By INCAT disability score 3 (37.5 %) patients had 0 points (normal), 3 (37.5 %) patients – 1 point and 1 patient had 2 (25 %) points. Nerve conduction study showed multifocal, asymmetric demyelinating changes in motor nerves. For the whole period of observation all patients were stable, so no one required pathogenic therapy.Conclusion. Chronic inflammatory demyelinating polyneuropathy is a clinically heterogeneous disorder, required clinical suspicion in all patients over 50 years with features of multiple nerve involvement; nerve conduction study helps to detect typical changes, including subclinical ones. The primary strategy of management typical and atypical disease variants in stable course and minimal symptoms is a case follow-up with precise assessment of advantages and disadvantages of pathogenic therapy.
Clinical and genetic characteristics of Charcot–Marie–Tooth disease type 4D (type Lom) in Russia
Authors: Murtazina A. F. , Shchagina O. A. , Milovidova T. B. , Dadali E. L. , Rudenskaya G. E. , Kurbatov S. A. , Fedotova T. V. , Nikitin S. S. , Sparber P. A. , Orlova M. D. , Polyakov A. V. 
Published: August 2020

Introduction. Charcot–Marie–Tooth disease type 4D is a hereditary demyelinating neuropathy, that occurs with the high frequency in patients of Roma origin. It is characterized by early onset at the age of 2–10 years and hearing impairment, manifested by the 3rd decade of life.Aim of the study. To describe the clinical and genetic characteristics of Charcot–Marie–Tooth disease type 4D in Russian patients of Roma origin.Materials and methods. For 14 probands from unrelated families of Roma origin with a clinical diagnosis of Charcot–Marie–Tooth disease, genetic tests for the pathogenic variants c. 442C>T in the NDRG1 gene and c. 3325C>T in the SH3TC2 gene was carried out. For 8 patients with Charcot–Marie–Tooth disease type 4D, detailed clinical and electrophysiological examination was performed.Results. In 11 families of Roma origin, the c. 442C>T pathogenic variant in the NDRG1 gene in a homozygous state was detected, which accounted for 79 % all observed Roma patients with Charcot–Marie–Tooth disease. There are 12 of the 14 tested families live in the European part of Russia, 7 of them are from nearby regions. The average age of onset was 3.3 years. The first symptom in 7 of 8 patients was gait disturbances. At the time of examination (age range 6–19 years), all patients showed marked hypotrophy and weakness of the feet, lower leg, hands muscles, feet deformities, reduction or loss of tendon reflexes.Discussion. Due to the detection of only one pathogenic variant in most Russian patients of Roma origin with Charcot–Marie–Tooth disease, the knowledge of the ethnicity of a proband with early myelinopathy can significantly simplify the confirmation of the diagnosis on the molecular level.
Kredi Derecelendirmenin Borsa Endeksleri Üzerindeki Etkileri: T-BRICS Ülkeleri Üzerine Bir Araştırma
Authors: Ovalı Musa , Kocabıyık Turan , Geyikçi Umut Burak 
Published: August 2020

Bu çalışmanın amacı derecelendirme kuruluşları tarafından verilen ülke derecelendirme notlarının ülkelerin borsa endeksleri üzerindeki etkilerini ortaya koymaktır. Bu amaçla, S&amp;P, Moody’s ve Fitch derecelendirme kuruluşları tarafından 2004-2019 yılları arasında T-BRICS ülkelerine verilen derecelendirme notlarının hisse senedi piyasaları üzerine etkileri olay çalışması yöntemiyle incelenmiştir. Anormal getirilerin varlığı, olayın etkilerini açık bir şekilde ortaya koyabilmek amacıyla ±15, ±10, ±5 ve ±1 günlük olay pencereleri dahilinde incelenmiştir. Analiz sonucunda bazı ülkelerin borsa endekslerinde negatif (pozitif) olayla aynı yönde negatif (pozitif) anormal getiriler tespit edilmiştir. Ek olarak bazı ülkelerin ise coğrafi konumları itibarıyla aynı olaylar karşısında farklı tepkiler verebildiği sonucuna ulaşılmıştır. Analiz sonucunda not duyurularının istatistiki olarak anlamlı kümülatif anormal getiri, ortalama anormal getiri ve kümülatif ortalama anormal getiriye yol açtığı tespit edilmiş, ilgili ülkelerdeki mevcut piyasaların yarı-etkin piyasa formunda etkin olmadığı sonucuna ulaşılmıştır. Anahtar Kelimeler: Kredi Derecelendirme, Ülke Kredi Derecelendirme, T-BRICS, Olay Çalışması, Anormal Getiri, Kümülatif Anormal Getiri.	JEL Sınıflandırması: G14, G24, N20
Geographical variation in determinants of high-risk fertility behavior among reproductive age women in Ethiopia using the 2016 Demographic and Health Survey: a geographically weighted regression analysis.
Authors: Tadesse Zemenu Tessema , Azanaw Melkalem Mamuye , Asmare Yeaynmarnesh , Gelaye Kassahun Alemu 
Published: August 2020

Abstract
Background: Maternal and child mortalities are the main public health problems worldwide and both are the major health concern in developing countries such as Africa and Asia. The fertility behavior of women characterized by maternal age, birth spacing, and order, impacts the health of women and children. The aim of this study was to assess the geographically variation in risk factors of high-risk fertility behavior (HRFB) among reproductive-age women in Ethiopia using the 2016 Demographic and Health Survey.Methods: A total of 11,022 reproductive-age women were included in this study. The data were cleaned and weighted by STATA 14.1 software. Bernoulli based spatial scan statistics was used to identify the presence of pure high-risk fertility behavior spatial clusters using Kulldorff’s SaTScan version 9.6 software. ArcGIS 10.7 was used to visualize the spatial distribution of high-risk fertility behavior. Geographically weighted regression analysis was employed by multiscale geographical using Multiscale geographical weighted regression version 2.0 software. A p-value of less than 0.05 was used to declare statistically significant predictors (at a local level). Results: Overall, 76 % with 95 % confidence interval of 75.60 to 77.20 of reproductive age women were faced with high-risk fertility problems in Ethiopia. High-risk fertility behavior was highly clustered in the Somali and Afar regions of Ethiopia. SaTScan identified 385 primary spatial clusters (RR= 1.13, P < 0.001) located at Somali, Afar, and some parts of Oromia Regional Stateregional state of Ethiopia. Women who are living in primary clusters were 13% more likely venerable to high-risk fertility behavior than outside the cluster. In geographically weighted regression, not using contraceptives and home delivery were statistically significant vary risk factors affecting high- risk fertility behavior spatially. No contraceptive use and home delivery were statistically significant predictors ( at the local level) in different regions of Ethiopia.Conclusion: In Ethiopia, HRFB varies across regions. Statistically, a significant-high hot spot high-risk fertility behavior was identified at Somali and Afar. No contraceptive use and home delivery were statistically significant predictors (at a local level) in different regions of Ethiopia. Therefore, policymakers and health planners better to design an effective intervention program at Somali, and Afar to reduce high-risk fertility behavior and Special attention needs about health education on the advantage of contraceptive utilization and health facility delivery to reduce high-risk fertility behavior.
Insecticide resistance in indoor and outdoor-resting Anopheles gambiae in Northern Ghana
Authors: Hamid-Adiamoh Majidah , Amambua-Ngwa Alfred , Nwakanma Davis , D’Alessandro Umberto , Awandare Gordon A. , Afrane Yaw A. 
Published: August 2020

Abstract
Background Selection pressure from continued exposure to insecticides drives development of insecticide resistance and changes in resting behaviour of malaria vectors. There is need to understand how resistance drives changes in resting behaviour within vector species. The association between insecticide resistance and resting behaviour of Anopheles gambiae sensu lato (s.l.) in Northern Ghana was examined. Methods F1 progenies from adult mosquitoes collected indoors and outdoors were exposed to DDT, deltamethrin, malathion and bendiocarb using WHO insecticide susceptibility tests. Insecticide resistance markers including voltage-gated sodium channel (Vgsc)- 1014F, Vgsc-1014S, Vgsc-1575Y, glutathione-S-transferase epsilon 2 (GSTe2)-114T and acetylcholinesterase (Ace1)-119S, as well as blood meal sources were investigated using PCR methods. Activities of metabolic enzymes, acetylcholine esterase (AChE), non-specific β-esterases, glutathione-S-transferase (GST) and monooxygenases were measured from unexposed F1 progenies using microplate assays. Results Susceptibility of Anopheles coluzzii to deltamethrin 24hr post-exposure was significantly higher in indoor (mortality=5%) than outdoor (mortality=2.5%) populations (P=0.02). Mosquitoes were fully susceptible to malathion (mortality: indoor=98%, outdoor=100%). Susceptibility to DDT was significantly higher in outdoor (mortality=9%) than indoor (mortality=0%) mosquitoes (P=0.006). Mosquitoes were also found with suspected resistance to bendiocarb but mortality was not statistically different (mortality: indoor=90%, outdoor=95%. P=0.30). Frequencies of all resistance alleles were higher in F1 outdoor (0.11-0.85) than indoor (0.04-0.65) mosquito populations, while Vgsc-1014F in F0 An. gambiae sensu stricto (s.s) was significantly associated with outdoor-resting behaviour (P=0.01). Activities of non-specific β-esterase enzymes were significantly higher in outdoor than indoor mosquitoes (Mean enzyme activity: Outdoor=: 1.70/mg protein; Indoor=1.35/mg protein. P<0.0001). AChE activity was also more elevated in outdoor (0.62/mg protein) than indoor (0.57/mg protein) mosquitoes but this was not significant (P=0.08). Human blood index (HBI) was predominantly detected in indoor (18%) than outdoor mosquito populations (3%). Conclusions The overall results did not establish that there was a significant preference of resistant malaria vectors to solely rest indoors or outdoors, but varied depending on the resistant alleles present. Phenotypic resistance was higher in indoor than outdoor-resting mosquitoes, but genotypic and metabolic resistance levels were higher in outdoor than the indoor populations. Continued monitoring of changes in resting behaviour within An. gambiae s.l. populations is recommended.
Administration of Saccharomyces boulardii mafic-1701 improves feed conversion ratio, promotes antioxidant capacity, alleviates intestinal inflammation and modulates gut microbiota in weaned piglets
Authors: Zhang Wenxiu , Bao Chengling , Wang Jian , Zang Jianjun , Cao Yunhe 
Published: August 2020

Abstract
Background: Probiotics are used as a means to improve animal health and intestinal development. Saccharomyces boulardii ( S. boulardii ) is a well-known probiotic, however, few studies have examined the effects of S. boulardii on weaned pig let performance. Therefore, this 28-day study compared the effects of S. boulardii mafic-1701 and aureomycin in weaned piglet diets on growth performance, antioxidant parameters, inflammation and intestinal microbiota. One hundred and eight weaned piglets were randomly divided into three dietary treatment groups: (1) basal diet (CON); (2) basal diet supplemented with 75 mg/kg aureomycin (ANT); (3) basal diet supplemented with 1 × 10 8 CFU/kg S. boulardii mafic-1701 (SB). Results: Compared to CON group, SB group had higher feed efficiency ( P < 0.01) and lower diarrhea rate ( P < 0.05) over the entire 28 days. Total superoxide dismutase concentration in serum was markedly increased in SB group ( P < 0.05). Moreover, compared with CON group, SB group decreased the level of pro-inflammatory cytokines interleukin-6 ( P < 0.01) and tumor necrosis factor ( P < 0.05) in jejunum. Supplementation with S. boulardii mafic-1701 increased abundance of Bacillus and Ruminococcaceae_UCG_009 ( P < 0.05), whereas abundance of unclassified _Clostridiaceae_4 was decreased ( P < 0.05). Furthermore, S. boulardii mafic-1701 administration increased cecal concentration of microbial metabolites , isobutyrate and valerate ( P < 0.05). Conclusions: The improvement in feed conversion ratio, reduction in diarrhea rate in weaned piglets provided diets supplemented with S. boulardii mafic-1701 may be associated with enhanced antioxidant activity, anti-inflammatory responses and improved intestinal microbial ecology.
COVID-19: A scoping review
Authors: Berhe Brhane , Legese Haftom , Degefa Hagos , Adhanom Gebre , Gebrewahd Aderajew , Mardu Fitsum , Tesfay Kebede , Kahsu Getachew , Welay Miglas , Negash Hadush 
Published: August 2020

Abstract
Background: Globally, the novel coronavirus, SARS-CoV-2, has led to a pandemic in which individuals may experience severe and life-threatening complications. Methods: A scoping review was conducted following the methodological framework. In this scoping review, 70 records as of May 21, 2020, were included and discussed to better understand the current updates of the virus. PubMed, BioRxiv, MedRxiv, Global Health and google scholars were searched comprehensively for articles, preprints, grey literature, reports, conference proceedings and expert information. Studies conducted in human and published in the English language were included in the review. All the findings and statements of the review regarding the outbreak are based on published data.Results: We identified 624 records, of which 70 studies met the inclusion criteria. We synthesized the data from the included records and deep insights were extracted. It was observed that the impact of the outbreak is worsening due to overcrowding, presence of asymptomatic carriers, scarcity of test kits, the immune escaping ability of the virus and lack of community awareness.Conclusions and recommendations: Due to the fast-spreading nature of the SARS-CoV-2 the prevention and control strategies become challenging. It is imposing social, psychological, and socio-economic impacts. Adherence to physical distancing, quarantining suspects, using personal protective equipment, health education and introducing appropriate handwashing practices, avoiding contact with animals, improved control, and prevention strategies are recommended.
Distraction by a cognitive task has a higher impact on electrophysiological measures compared with conditioned pain modulation
Authors: Do A. T. Lisa , Enax-Krumova Elena , Özgül Özüm , Eitner Lynn B. , Heba Stefanie , Tegenthoff Martin , Maier Christoph , Höffken Oliver 
Published: August 2020

Abstract
Background Conditioned pain modulation (CPM) evaluates the effect of a painful conditioning stimulus (CS) on a painful test stimulus (TS). Using painful cutaneous electrical stimulation (PCES) as TS and painful cold water as CS, the pain relief was paralleled by a decrease in evoked potentials (PCES-EPs). We now aimed to compare the effect of CPM with cognitive distraction on PCES-induced pain and PCES-EP amplitudes. Methods PCES was performed using surface electrodes inducing a painful sensation of 60 (NRS 0-100) on one hand. In a crossover design healthy subjects (included: n=38, analyzed: n=23) immersed the contralateral hand into 10°C cold water (CS) for CPM evaluation and performed the 1-back task for cognitive distraction. Before and during the CS and 1-back task, respectively, subjects rated the pain intensity of PCES and simultaneously cortical evoked potentials were recorded. Results Both CPM and cognitive distraction significantly reduced PCES-EP amplitudes (CPM: 27.6±12.0μV to 20.2±9.5μV, cognitive distraction: 30.3±14.2µV to 13.6±5.2μV, p<0.001) and PCES-induced pain (on a 0–100 numerical rating scale: CPM: 58±4 to 41.1±12.3, cognitive distraction: 58.3±4.4 to 38.0±13.0, p<0.001), though the changes in pain intensity and PCES-amplitude did not correlate. The changes of the PCES-EP amplitudes during cognitive distraction were more pronounced than during CPM (p=0.001). Conclusions CPM and cognitive distraction reduced the PCES-induced pain to a similar extent. The more pronounced decrease of PCES-EP amplitudes after distraction by a cognitive task implies that both conditions might not represent the general pain modulatory capacity of individuals, but may underlie different neuronal mechanisms with the final common pathway of perceived pain reduction.