bluuebunny/crossref_metadata_2025_split huggingface dataset:
High expression of sperm-associated antigen 5 correlates with poor survival in ovarian cancer
Authors: Zhang Mei , Sha Ling , Hou Ning , Shi Chuanbing , Tan Lin 
Published: January 2020

Abstract
Objectives: Sperm-associated antigen 5 (SPAG5), a spindle-binding protein, regulates the process of mitosis. The present study focused on the relationship between SPAG5 expression and the clinicopathological characteristics and prognosis of ovarian cancer. Methods: First, we used the Gene Expression Omnibus (GEO) database to analyze SPAG5 expression in ovarian cancer and its clinical relevance. Subsequently, qPCR test was used to detect SPAG5 mRNA expression in 20 cases of ovarian cancer. The expression of SPAG5 protein in a tissue microarray containing 102 cases of ovarian cancer was detected by immunohistochemistry. Cox regression and Kaplan–Meier survival analyses were performed to identify the prognostic factors for the 102 ovarian cancer patients. Results: In the GEO datasets, SPAG5 mRNA expression was significantly higher in ovarian cancer tissues than that in normal ovarian tissues (P &lt; 0.001). qPCR and immunohistochemistry showed that SPAG5 expression in ovarian cancer tissues was significantly higher than that in paracancerous tissues (P = 0.002, P &lt; 0.001). The high expression of SPAG5 in ovarian cancer was correlated with histological type (P = 0.009), lymph node metastasis (P = 0.001), distant metastasis (P = 0.001), TNM stage (P = 0.001), and prognosis (P = 0.001). The Kaplan–Meier curve indicated that rates of disease-free survival (DFS) and overall survival (OS) were even lower in patients with high SPAG5 expression. Multivariate analysis showed that SPAG5 expression (P = 0.001) and TNM staging (P = 0.002) were independent prognostic factors for the DFS of ovarian cancer. Conclusions: These results suggest that high SPAG5 expression was correlated with multiple clinicopathological features of ovarian cancer and can be used as an evaluation indicator for a poor ovarian cancer prognosis.
Neuron-derived orphan receptor-1 modulates cardiac gene expression and exacerbates angiotensin II-induced cardiac hypertrophy
Authors: Cañes Laia , Martí-Pàmies Ingrid , Ballester-Servera Carme , Herraiz-Martínez Adela , Alonso Judith , Galán María , Nistal J. Francisco , Muniesa Pedro , Osada Jesús , Hove-Madsen Leif , Rodríguez Cristina , Martínez-González José 
Published: January 2020

Abstract
Hypertensive cardiac hypertrophy (HCH) is a common cause of heart failure (HF), a major public health problem worldwide. However, the molecular bases of HCH have not been completely elucidated. Neuron-derived orphan receptor-1 (NOR-1) is a nuclear receptor whose role in cardiac remodelling is poorly understood. The aim of the present study was to generate a transgenic mouse over-expressing NOR-1 in the heart (TgNOR-1) and assess the impact of this gain-of-function on HCH. The CAG promoter-driven transgenesis led to viable animals that over-expressed NOR-1 in the heart, mainly in cardiomyocytes and also in cardiofibroblasts. Cardiomyocytes from TgNOR-1 exhibited an enhanced cell surface area and myosin heavy chain 7 (Myh7)/Myh6 expression ratio, and increased cell shortening elicited by electric field stimulation. TgNOR-1 cardiofibroblasts expressed higher levels of myofibroblast markers than wild-type (WT) cells (α 1 skeletal muscle actin (Acta1), transgelin (Sm22α)) and were more prone to synthesise collagen and migrate. TgNOR-1 mice experienced an age-associated remodelling of the left ventricle (LV). Angiotensin II (AngII) induced the cardiac expression of NOR-1, and NOR-1 transgenesis exacerbated AngII-induced cardiac hypertrophy and fibrosis. This effect was associated with the up-regulation of hypertrophic (brain natriuretic peptide (Bnp), Acta1 and Myh7) and fibrotic markers (collagen type I α 1 chain (Col1a1), Pai-1 and lysyl oxidase-like 2 (Loxl2)). NOR-1 transgenesis up-regulated two key genes involved in cardiac hypertrophy (Myh7, encoding for β-myosin heavy chain (β-MHC)) and fibrosis (Loxl2, encoding for the extracellular matrix (ECM) modifying enzyme, Loxl2). Interestigly, in transient transfection assays, NOR-1 drove the transcription of Myh7 and Loxl2 promoters. Our findings suggest that NOR-1 is involved in the transcriptional programme leading to HCH.
Deliberate Supervision: Practical Strategies for Success
Authors: Kleinhans Kelly A. , Brock Christina , Bland Lauren E. , Berry Bethany A. 
Published: January 2020

PurposeClinical supervisors play a fundamental role in enabling students to transform knowledge into clinical skills. The 2020 changes to Speech-Language Pathology Certification Standards will require speech-language pathologists who want to serve as clinical supervisors of applicants for certification to complete a minimum of 9 months of practice experience postcertification and 2 hr of professional development in the professional practice domain of supervision postcertification prior to overseeing a student in a clinical supervisor capacity.ConclusionThis article describes a framework for clinical supervisors of graduate students to use based on the premise that supervision should be an intentional reflective activity. The authors describe how to plan for clinical education across practice settings, provide appropriate feedback, and use questions effectively.Supplemental Materialhttps://doi.org/10.23641/asha.11528250
Optical spectroscopy of type 2 LINERs
Authors: Hermosa Muñoz L. , Cazzoli S. , Márquez I. , Masegosa J. 
Published: January 2020

Context.Type 2 Low-ionization Nuclear Emission-line Regions (LINERs) have been optically classified with the Palomar data as not presenting a broad component in the Balmer emission lines that are associated with the broad-line region (BLR) of the active galactic nuclei (AGN).Aims.We aim to unveil the presence of different kinematic components of emission lines in the nuclear region of a sample of local (z ≤ 0.022) type 2 LINERs. We focus on the analysis of the true nature of LINERs by means of the detection (or nondetection) of a broad component that originated in the BLR of the AGN. Additionally, we search for the possible presence of nonrotational motions such as outflows in these LINERs.Methods.We applied a decomposition of the nuclear emission lines using an spectroscopic analysis of the optical spectra of nine type 2 LINERs of intermediate-resolution spectroscopic data retrieved from theHubbleSpace Telescope (HST) archive. The study is completed with archival spectra from the Double Spectrograph from the Palomar Observatory.Results.The emission line fitting reveals the presence of a broad component associated with the BLR in six out of the nine galaxies for the space-based data, and for two out of the eight from the ground-based spectra. The velocity dispersion for two galaxies (NGC 4486 and NGC 4594) measured in HST/STIS data suggest the presence of outflows.Conclusions.The results indicate that the spatial resolution plays a major role in the detection of the BLR, as it appears diluted in the ground-based data (even after removing stellar contribution). This is also true for the emission line diagnostics, as the contaminant light contributes to lower emission line ratios toward the star-forming area of standard BPTs. We propose to reclassify NGC 4594 as a type 1 LINER, since a BLR component is seen in both space- and ground-based spectra. We find ambiguous results for the BLR component of NGC 4486. The modest outflow detection in our sample may indicate that they are not as frequent as seen for type 1 LINERs.
Vagusnervstimulation bei affektiven Störungen
Authors: Dibué-Adjei Maxine , Kamp Marcel Alexander , Vogelsang Jonathan , Wiltfang Jens , Wolff-Menzler Claus 
Published: January 2020

ZusammenfassungVagusnervstimulation (VNS) ist ein minimalinvasives Neurostimulationsverfahren und wurde 1994 in Europa für die Behandlung von Kindern und Erwachsenen mit medikamentenresistenten Epilepsien zugelassen. Die Beobachtung, dass sich Depressionen, die häufigste Komorbidität der Epilepsie, signifikant unter VNS verbesserten, initiierte Studien der VNS bei Patienten mit therapieresistenten Depressionen (TRD), die 2001 zu der europäischen Zulassung der VNS für TRD führten. Heutzutage ist in Deutschland der Einsatz der VNS für TRD-Patienten auf wenige hochspezialisierte (oft universitäre) Zentren limitiert und das Verfahren in der psychiatrischen Praxis weitestgehend unbekannt. In dieser systematischen Übersicht identifizieren und diskutieren wir deshalb die aktuellsten Studien zu VNS bei TRD sowie Empfehlungen von Fachgesellschaften und diskutieren den Einsatz der VNS in der klinischen Praxis. In den letzten fünf Jahren wurden fünf Studien mit Evidenzgrad 2 und vier Studien mit Evidenzgrad 3 zu der Wirkung von VNS bei TRD-Patienten publiziert. Kurzfristige Wirksamkeit der VNS konnte in klinischen Studien nicht gezeigt werden. Mehrere Studien demonstrieren die Langzeitwirksamkeit der VNS. Die kürzlich publizierte größte Untersuchung der Thematik bestätigt signifikant bessere Behandlungsergebnisse bei Patienten, die eine Zusatztherapie mit VNS erhielten – im Vergleich zu Patienten, die nur Behandlung nach Goldstandard über fünf Jahre erhielten. Die langfristige Wirksamkeit der VNS bei TRD-Patienten ist mit Evidenz des Grades 2 belegt, jedoch ist es unklar, welche TRD-Patienten die höchste Wahrscheinlichkeit eines Ansprechens auf VNS haben, was die Patientenselektion in der Praxis erschweren kann. Zusätzlich wirkt sich möglicherweise die unklare und variable Definition der therapieresistenten Depression problematisch auf den rechtzeitigen Einsatz von Neurostimulationsverfahren aus.
Preparation and Characterization of Albumin Nanoparticles of Paclitaxel-Triphenylphosphonium Conjugates: New Approach to Subcellular Targeting
Authors: Hosseinifar Nastaran , Goodarzi Navid , Sharif Amir Abdolah Mehrdad , Amini Mohsen , Esfandyari-Manesh Mehdi , Dinarvand Rassoul 
Published: January 2020

AbstractMitochondria have been recognized as important targets in cancer therapy due to their role in the respiratory process of cells. One approach employed for mitochondrion targeting is conjugation of a delocalized cation such as triphenylphosphonium (TPP), with antineoplastic agents, for instance paclitaxel (PTX). In cell cytoplasm, TPP-PTX can come close to mitochondria due to its high positive charge, which has a strong tendency toward the enhanced negative charge of mitochondria. The esteric bond of TPP-PTX can break down in the acidic environment of tumor cells and release the PTX, which can act directly on mitochondria to kill tumor cells. TPP-PTX was synthesized in three steps: Succinic anhydride (SUC) reacted with PTX to achieve succinyl paclitaxel (SUC-PTX), which has an acid-labile esteric bond. Then 2-triphenylphosphonium ethylammonium (ATPP) was prepared by attaching 2-bromoethylammunium bromide to TPP. Finally, a TPP-PTX prodrug was synthesized by attaching these materials. The products of all steps were characterized by thin-layer chromatography (TLC), infrared spectroscopy (IR), and nuclear magnetic resonance (1H NMR, 13C NMR). The purity of the products was determined by HPLC methods. TPP-PTX, as a prodrug, was loaded in to human serum albumin (HSA) nanoparticles by a method inspired by nab-technology with 130–160 nm particle size distribution, PdI=0.166 and Zeta potential −12.6 mV.
Morbidity Outcomes of Very Low Birth Weight Neonates Receiving Parenteral Nutrition with Fish Oil Enriched Lipid Emulsion or Lipid Emulsion with Soybean Oil: An Observational Study
Authors: Uberos José , Jiménez-Montilla Sara , Molina-Oya Manuel , Nieto-Gómez Pelayo , Millan Isabel Cubero 
Published: January 2020

AbstractIntralipid (Fresenius Kabi) was the most commonly used lipid emulsion in parenteral nutrition (PN), with a 100% soybean oil composition, a low vitamin E content, and a ω-6: ω-3 ratio of 7:1. A recent alternative formulation is SMOFlipid (Fresenius Kabi), with a ω-6: ω-3 ratio of 5:2 and higher vitamin E content. A retrospective observational study was conducted to determine neonatal morbidity in very low birth weight (VLBW) premature infants during two periods: P1, when PN was based exclusively on Intralipid, and P2, when only SMOFlipid was supplied. In total, 170 VLBW neonates were analyzed, of whom 103 received PN for more than 6 days, 56 during P1, and 47 during P2. In both periods, the antenatal and neonatal characteristics of the cohort were comparable. In this analysis, the prevalence of associated comorbidities was determined. During P2, there were fewer cases of moderate to severe bronchopulmonary dysplasia (BPD) and of cholestasis, but more cases of late sepsis, mainly Staphylococcus epidermidis. No changes in the prevalence of other neonatal comorbidities were observed. We believe that the SMOFlipid used in PN could discreetly improve the prevalence of cholestasis or BPD.
Step-Headache: A Distinct Symptom of Migraine
Authors: Pradhan Sunil , Das Animesh , Ghosh Jayashri 
Published: January 2020

Abstract

Objectives The diagnosis of migraine depends on various characteristics of headache with their associated constitutional symptoms such as nausea, vomiting, photophobia, and phonophobia. Relatively severe intensity, throbbing character, unilaterality, and aggravation with physical activity are the key features of migraine headache. We did this study to describe a new symptom (step-headache) in migraine in which some patients complained of uncomfortable or painful thump over the head with each footstep during walking or running.
Materials and Methods Self-designed proforma was filled in each clinically diagnosed patient of migraine or tension-type headache in an outpatient clinic setting. The symptom designated here as step-headache was evaluated in 150 patients of migraine including 26 patients with overlapping headache and 244 patients of tension-type headache. Binary logistic regression was used for association analysis of step-headache with subgroups of migraine and with other migraine features.
Statistical Analysis Frequency distributions were expressed as numbers (percentages) or mean ± standard deviation. Binary logistic regression was used for association analysis of step-headache with subgroups of migraine and with other migraine features.
Results Step-headache was experienced by 97 (64.67%) migraine patients with nearly equal distribution among the two clinical subtypes (61.5% for migraine with aura and 65.3% for migraine without aura) but with high prevalence among perimenopausal onset migraine. Of all the patients who had this symptom, 77.32% experienced it during all the migraine attacks. The step-headache was differentiable from throbs of migraine and their exacerbation during physical activity by its synchrony with footsteps and its presence during nonpulsatile headaches or nonheadache phases of migraine. Sensitivity of this symptom was 64.67% while specificity was 100%.
Conclusion Among primary headaches, step-headache is a less well-known but common and distinct symptom of migraine. It has good sensitivity and high specificity for migraine.
Imaging Evaluation of Disorders of Sex Development
Authors: Eapen Anu , Chandramohan Anuradha , Simon Betty , Putta Tharani , John Reetu , Kekre Aruna 
Published: January 2020

AbstractDisorders of sex development (DSD) refer to congenital conditions with a typical development of chromosomal, gonadal, or anatomic sex. In the revised classification of DSD, there are three categories based on karyotype: 46,XX DSD; 46,XY DSD; and sex chromosome DSD. Imaging, as part of a multidisciplinary approach to management of DSD, has a key role in gender assignment. The main role of imaging is to help in identifying the gonads and the Müllerian structures. Ultrasound is useful, especially in the neonate with ambiguous genitalia. Magnetic resonance imaging is a useful modality to locate and characterize the gonads in young girls with primary amenorrhea and also to identify streak gonads, which have a risk of malignancy.
A Low 50-gram, 1-hour Glucose Challenge Test Value Predicts Neonatal Birth Weight Less than the 10th Percentile: A Systematic Review and Meta-Analysis
Authors: Mitta Melanie , Sanchez-Ramos Luis , Roeckner Jared T. , Bennett Stevie , Kovacs Andrew , Kaunitz Andrew M. 
Published: January 2020

Abstract
Objective This study aimed to perform a systematic review with meta-analysis to investigate if women with a low 50-g, 1-hour glucose challenge test (GCT) value are at risk for having neonates with a birth weight less than the 10th percentile.
Study Design A computerized literature search was conducted to identify studies that compared outcomes of pregnant women with a low GCT value versus women with a normal GCT value during routine screening for gestational diabetes.
Results Sixteen cohort studies were included for analysis. Women with a low GCT value were noted to have a 43% increased odds of having neonates with birth weight less than the 10th percentile (odds ratio [OR]: 1.43; 95% confidence interval [CI]: 1.28–1.60) and 30% increased odds of having neonates with a birth weight less than 2,500 g (OR: 1.3; 95% CI: 1.0–1.7) when compared with women with a normal GCT value. The rates of preterm delivery, neonatal intensive care unit (NICU) admission, pregnancy-induced hypertension (PIH)/preeclampsia, respiratory distress, NICU, and Apgar scores less than 7 were similar in the two groups.
Conclusion A low GCT value defined as less than 90 mg/dL identifies pregnancies at elevated risk for having neonates with a birth weight less than the 10th percentile.
How I Manage Cancer-Associated Thrombosis
Authors: Moik Florian , Ay Cihan 
Published: January 2020

AbstractIn this concise review, we discuss some common clinical challenges in the management of patients with cancer-associated venous thromboembolism (VTE), a frequent complication in patients with cancer that increases morbidity and mortality. While direct oral anticoagulants (DOACs) have been established in clinical practice for anticoagulation in patients with VTE without cancer, their efficacy and safety in patients with cancer have not been assessed in randomized controlled trials until recently. The choice of the appropriate anticoagulant agent in the era of DOACs to treat patients with cancer-associated VTE is based on balancing the risk of recurrence against the risk of bleeding, and potential drug–drug interactions. However, the management of patients is challenged by special scenarios such as incidentally diagnosed pulmonary embolism and catheter-related thrombosis, and sometimes complicated by concomitant thrombocytopenia. We provide guidance for management of cancer-associated VTE in different clinical scenarios in a case-based manner and briefly review recent clinical studies and guidelines to explain our approach to management of the cases.
A pragmatics of intimacy
Authors: Márquez Reiter Rosina , Frohlich David M. 
Published: January 2020

AbstractThis study examines the ways in which multiple modern communication technologies facilitate, across time and space, the maintenance of a close interpersonal relationship between two best friends. The analysis, which focuses mainly on the openings and closings of the different types of communications, reveals a tendency for the friends to shorten openings and extend closings. However, this is possible only if the friends are fully aware of, and care about, the practical, social and emotional details of each other’s lives during periods of absence. The concomitant linguistic behaviours in their interpersonal interactions could be described as a kind of pragmatics of intimacy which cannot be achieved without the explicit and practical demonstration of that mutual care and concern.
Autophagy promotion enhances the protective effect of Morroniside on human OA chondrocyte
Authors: Xiao Zhanhao , Wang Jiankun , Chen Sunyu , Feng Yang 
Published: January 2020

Abstract
Morroniside plays a therapeutic role in knee osteoarthritis (OA) by protecting chondrocytes. PI3K/AKT signaling is involved in the regulation of chondrocytes by Morroniside. PI3K/AKT suppresses autophagy through downstream signaling. However, the regulation of chondrocyte autophagy by Morroniside and the significance of the above effect on protecting chondrocytes aren’t clear. The results showed that Morroniside inhibited the autophagiy of human OA chondrocytes. Besides, both PI3K inhibitors and mTOR inhibitors significantly reversed the autophagy reduced by Morroniside, but had no effect on the protective effect of Morroniside on chondrocytes. However, the enhanced autophagy caused by overexpression of autophagic genes enhanced the protective effect of Morroniside on chondrocytes. In conclusion, Morroniside represses the autophagy of human OA chondrocyte, which is related to PI3K/mTOR pathway. Moreover, the upregulation of autophagy further promoted the role of Morroniside in treating chondrocytes. Our data present a potential clue for the therapeutic strategies of Morroniside in treating OA.
Suppression of NSDHL attenuates adipogenesis with a downregulation of LXR-SREBP1 pathway in 3T3-L1 cells
Authors: Zhang Haiyan , Li Chengping , Xin Youzhi , Cui Xiao , Cui Jianwei , Zhou Guoli 
Published: January 2020

Abstract
Previous RNA-Seq analyses revealed that NAD(P)H steroid dehydrogenase-like (NSDHL) has a different expression during 3T3-L1 differentiation; however, its roles in adipogenesis are unknown. In the present study, using quantitative real-time PCR, we confirmed that NSDHL knockdown increased the proliferation of 3T3-L1 preadipocytes, but attenuated the differentiation of 3T3-L1 preadipocytes, as evidenced by reduced lipid accumulation and down-regulation of PPARγ gene expression. Further analyses showed that the expression peak of NSDHL was at the early stage of 3T3-L1 preadipocytes differentiation and LXR-SREBP1 signaling pathway was downregulated in NSDHL-knockdown 3T3-L1 cells. Collectively, our findings indicate that NSDHL is a novel modulator of adipogenesis. Moreover, our data provide insight into the complex relationships between sterol sensing, LXR-SREBP1 signaling pathway, and PPARγ in 3T3-L1 cells.
Quantum field representation of photon-molecule interactions
Authors: Bradshaw David S , Forbes Kayn A , Andrews David L 
Published: January 2020

Abstract
In reporting and explaining the interactions of light with atoms and molecules, a photon-based description is not only appropriate but clearly essential when electronic or other kinds of quantum transition ensue. However, textbook treatments frequently go no further than the Planck relation, in representing the quantum nature of the light itself—often resorting to classical principles when discussing mechanism. Complete consistency and rigour can be achieved by treating both the matter and the radiation in a fully quantised form, which requires the electromagnetic fields to be cast in a quantum field representation. It proves possible to develop a rigorous approach to this theory that is simple to convey and apply, and which lends itself to a significantly enhanced level of appreciation of mechanism. This paper lays a concise foundation and exemplifies the application in three specific cases: absorption, emission and scattering. It is also shown how this formulation affords a basis for applications in higher-order, multiphoton and nonlinear optical processes.