bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characteristics of Endoscopic and Pathological Findings of Amebic Colitis
Authors: Meng Ying , Yue Bing , Zhou Hua Yan , Zhao Ying Hai , Wu Dong Yong , zong ye 
Published: November 2020

Abstract
Background The clinical features of amoebic colitis resemble those of Inflammatory Bowel Disease (IBD), and therefore the risk of misdiagnosis is very high.Methods We retrospectively reviewed data of all amebic colitis cases admitted to Beijing Friendship Hospital from January 2015 to January 2020. Cases were diagnosed by clinical presentation, laboratory examinations, and colonoscopy with biopsy and histological examination. Results 16 patients were diagnosed with amebic colitis by colonoscopies accompanied by biopsies and microscopic examinations. At first, 12 (75%) patients were misdiagnosed with IBD. The cecum was the most common site of amebic colitis (100%), and the caecum and rectum were also involved in many lesions (68.75%). Multiple lesions of erosion and/or ulcers were recognized in all patients (100%). The features of endoscopic findings included multiple irregularly shaped ulcers and erosions with surrounding erythema, and the ulcers and erosions were covered by the white or yellow exudates. The intervening mucosae between the ulcers or erosions were normal. The features of the rectums can be divided to 2 types: in 6 patients (54.5%), the irregular ulcers or erosions covered with white or yellow exudates were observed in the rectum and the cecum, and the bloody exudates in the rectum were more severe than those in the cecum; in the other 5 patients (45.5%), rectal lesions were much less severe than those in the cecum, and small superficial erosions or reddened mucosa were observed in the rectal ampulla. All patients were diagnosed as detection of amebic trophozoites from HE-stained biopsy specimens. The number of trophozoites ranged from 1 /HPF to ＞50/HPF. Among 16 cases, mild architectural alteration of colon crypt was observed in 10 cases (62.5%), and serious architectural alteration of colon crypt was found which had a crypt branch in 1 case (16.7%). Cryptitis was observed in 12 cases (75%) and its severity was mild or moderate. No crypts abscesses were observed in all cases. Conclusions Colonoscopies with histological examinations are very important to diagnose amebic colitis. Detecting the amoebic trophozoites in the exudates by histological examination is vital. Sometimes a negative biopsy does not rule out amebiasis, repeated biopsies may be needed to make the diagnosis.
Bioinformatic Identification and Validation of Key Genes and Biological Pathways Involved in Hepatitis B Virus-related Hepatocellular Carcinoma
Authors: Ren Guan-Hua , Mei Fan-Biao , Zhang Chao-Jun , Cai Dong-Mei , Long Long , Li Ke-Zhi , Huang Tian-Ren , Deng Wei 
Published: November 2020

Abstract
Objectives: Hepatocellular carcinoma (HCC) is a common malignant tumor severely scathing human health. As we all know, one of the main risk factors of HCC is chronic hepatitis B virus (HBV) infection, which involved in the oncogenesis of HCC through direct and indirect mechanisms such as inflammatory injury, integration into the host genome and interaction with some special target genes. This study aimed to identify these key genes potentially associated with HBV-related HCC by bioinformatics analyses.Results: Total of 320 DEGs was identified between HBV-related HCC tissue samples and adjacent normal samples. These DGEs were strongly associated with several biological processes, such as retinol metabolism and steroid hormone biosynthesis. A PPI network was constructed and top six hub genes, including CDK1, CCNB1, CDC20, CDKN3, HMMR and MKI67, were determined. GEPIA online tool analysis validated the six key hub genes had the same expression trend as predicted in The Cancer Genome Atlas (TCGA) datasets. The overall survival and disease-free survival reflected that high expression of CDK1, CDC20, HMMR, MKI67 and CCNB1 significantly predicted poor prognosis, whereas CDKN3 expression has no statistical differences in overall survival.Conclusion: The present study identified key genes and pathways involved in HBV-related HCC, which will improve our understanding of the mechanisms underlying the development and recurrence of HCC. The six identified genes might be potential biomarkers for the diagnosis and treatment of HBV-related HCC.
Doula Support in Office Hysteroscopy: Results From A Pilot Study
Authors: Montejo Rocio , Hermansson Jonas , Wranker Lena Sandin , Danielsson Louise 
Published: November 2020

Abstract
Background This pilot study aimed to evaluate the feasibility of doula support in office hysteroscopy and the potential effectiveness of doula support during office hysteroscopy to reduce anxiety and pain. Methods This study was designed as a two-armed pilot study, with an intervention and a control group, with participants allocated to each group in a 1:1 ratio. 30 of 58 patients were initially excluded from due to not could read Swedish or declining to participate in the study. Twenty-eight women with indications for office hysteroscopy were randomized to receive doula support (intervention) during the procedure or to routine care (control group). The data collected included general and gynecological data, Spielberg State-Trait Anxiety Inventory-S (STAI), and the Numeric Rate Scale (NRS) for pain intensity. Feasibility was measured in terms of successful office hysteroscopies, duration, and adverse events. Results Success rates, duration, and adverse events were similar between the groups, with no differences in reported pain intensity. Both groups had high, comparable levels of anxiety before the procedure, whereas after the procedure, the doula group showed slightly increased anxiety while the control group showed slightly decreased anxiety. There was a significant difference between groups favoring the control group when comparing STAI-S mean score post-procedure but not when analysing the mean change across groups. Conclusion Exploring a new take on doula support, this pilot study suggests that it may be feasible but not superior to routine care support in office hysteroscopy. Also, high anxiety levels may be more of an issue than pain during the procedure. Further investigation of the state and trait anxiety in different hysteroscopy populations and health care contexts is recommendedTrial Registration The study was registered in de Research Database Registry in Västra Götalandsregionen (Sweden) with number: 254161 on 10 November 2018.
Circulating Tumor Cells Predict Prognosis Following First-Generation EGFR-TKI Treatment in EGFR- and TP53-Mutant Non-Small Cell Lung Cancer
Authors: He Jing , Zhang Wei , Qian Huizhu , Liu Ping , Xu Jing , Gao Wen 
Published: November 2020

Abstract
Purpose: First-generation EGFR-TKIs have become the first-line standard treatment for advanced non-small cell lung cancer (NSCLC) with EGFR mutations. This study isolates and quantifies circulating tumor cells (CTCs) and evaluates patient prognosis before and after first-line treatment with EGFR-TKIs in advanced NSCLC with EGFR and TP53 mutation.Methods: Patients with advanced NSCLC with EGFR and TP53 mutation were treated with a first-generation EGFR-TKI using a standard daily dose. Continuous blood samples were collected at baseline (CTCs-d0) and 28 days (CTCs-d28), and the isolation by size of tumor cells (ISET) method was used to detect CTCs.Results: The CTC results were divided into favorable (< 5 CTCs) and unfavorable (≥ 5 CTCs) groups. The median progression-free survival (PFS) of patients in the favorable group was significantly longer at baseline compared to those in the unfavorable group (15 vs 7.5 months; p = 0.0055). After 28 days of treatment with first-generation EGFR-TKI, the PFS of patients in the favorable group was 12.5 months, which was significantly longer than the median PFS of 7 months in the unfavorable group (p = 0.0003). After treatment, the PFS of patients with reduced CTCs was significantly better than those with no significant change in CTCs (9 months vs 6 months, p = 0.014). In univariate and multivariate analysis, patients with CTCs-d0 ≥ 5 and CTCs-d28 ≥ 5 had significantly lower PFS when compared to those with CTCs-d0 < 5 and CTCs-d28 < 5, respectively.Conclusion: This study confirmed for the first time that CTC count is closely correlated with prognosis in EGFR- and TP53-mutant advanced NSCLC following first-line treatment with first-generation EGFR-TKIs.
Development of a Prognostic Gene Signature For Hepatocellular Carcinoma
Authors: Wu Cuiyun , Luo Yaosheng , Chen Yinghui , Qu Hongling , Zheng Lin , Yao Jie 
Published: November 2020

Abstract
Background: Accurate prediction of overall survival is important for prognosis and the assignment of appropriate personalized clinical treatment in hepatocellular carcinoma (HCC) patients. The aim of the present study was to establish an optimal gene model for the independent prediction of prognosis associated with common clinical patterns.Methods: Gene expression profiles and the corresponding clinical information of the LIHC cohort were obtained from The Cancer Genome Atlas. Differentially expressed genes were found using the R package “limma”. Subsequently, a prognostic gene signature was developed using the LASSO Cox regression model. Kaplan–Meier, log-rank, and receiver operating characteristic (ROC) analyses were performed to verify the predictive accuracy of the prognostic model. Finally, a nomogram and calibration plot were created using the “rms” package.Results: Differentially expressed genes were screened with threshold criteria (FDR < 0.01 and |log FC|>3) and 563 differentially expressed genes were obtained, including 448 downregulated and 115 upregulated genes. Using the LASSO Cox regression model, a prognostic gene signature was developed based on nine genes,IQGAP3, BIRC5, PTTG1, STC2, CDKN3, PBK, EXO1, NEIL3, and HOXD9, the expression levels of which were quantitated using RT-qPCR. According to the risk scores, patients were separated into high-risk and low-risk groups. Patients with lower risk scores generally had a better survival rate than those with higher risk scores. The mortality rate in the high-risk group was 42.02%, while that in the low-risk group was 12.50%. Results of the log-rank test showed significant differences in mortality between the two groups (HR: 4.86; 95% CI: 2.72–8.69; P = 1.01E-08). Subsequently, we assessed the prognostic accuracy of the gene signature using an ROC curve and the results show good sensitivity and specificity, with an average area under the curve (AUC) of 0.81 at 5 years (P < 0.01). Following multivariate adjustment for conventional clinical patterns, the prognostic gene signature remained a powerful and independent factor (HR: 4.70; 95% CI: 2.61–8.38; P = 2.06E-07), confirming its robust predictive ability of overall survival in HCC patients. Finally, a nomogram was established based on the gene signature and four clinicopathological features, which demonstrated an advantageous discriminating ability with the potential to facilitate clinical decision-making in HCC.Conclusion: Our prognostic gene signature can be used as a combined biomarker for the independent prediction of overall survival in HCC patients. Moreover, we created a nomogram that can be used to infer prognosis and aid individualized decisions regarding treatment and surveillance.
Vaspin Alleviates Pathological Cardiac Hypertrophy By Regulating Autophagy-Dependent Myocardial Senescence
Authors: Rui Haiying , Li Ruochuan , Liu Lulu , Han Ziqi , Yu Huaxiang , Qin Dandan , Gong Xiaohui , Cao Shengchuan , Yuan Qiuhuan , Xu Feng , Xue Li , Chen Yuguo 
Published: November 2020

Abstract
Background: Visceral adipose tissue derived serine protease inhibitor (vaspin), a secretory adipokine, was reported to play a protective role in insulin resistance. Recent studies have demonstrated that serum vaspin levels are downregulated in patients with coronary artery disease (CAD) and that vaspin has a protective effect on myocardial ischaemia/reperfusion injury (IRI) and atherosclerosis. However, whether vaspin exerts specific effects on pathological cardiac hypertrophy remains unknown.Methods: Pathologic cardiac hypertrophy was induced in male C57BL/6J wild type (WT) and vaspin knockout (vaspin ko) mice. Buparlisib (PI3K inhibitor, 50 mg/kg), rapamycin (mTOR inhibitor, 20 mg/kg), or A 769662 (AMPK agonist, 30 mg/kg) wer e pre and co administered to vaspin ko mice daily for a period of 15 days. Induction of pathological cardiac hypertrophy was performed by the subcutaneous administration of isoproterenol (ISO) (5 mg/kg) into mice from the 7th to the 15th day. Cardiac hype rtrophy, fibrosis, and cardiac function were examined in these mice. Critical characteristics of senescence (senescence associated β galactosidase activity and expression of cyclin dependent kinase inhibitors) were examined in the cardiac hypertrophy modelResults: We provide the first evidence that the serum level of vaspin decreased during pathological cardiac hypertrophy; further, knocking out of vaspin resulted in markedly exaggerated cardiac h ypertrophy and fibrosis, and increased cardiomyocyte senesc senescence in mice treated with ISO. Conversely, the administration of exogenous ence in mice treated with ISO. Conversely, the administration of exogenous recombinant human vaspin in vitro protected myocardial cells against hypertrophy recombinant human vaspin in vitro protected myocardial cells against hypertrophy and senescence caused by ISO. and senescence caused by ISO. MechanisticallyMechanistically, PI3K, PI3K--AKTAKT--mTOR mTOR pathwaypathway--dependent activation of autophadependent activation of autophagic flux was involved in the protective gic flux was involved in the protective effects of vaspin toward cardiac hypertrophy.effects of vaspin toward cardiac hypertrophy.Conclusion: Our results showed for the first time that vaspin functions as a critical Our results showed for the first time that vaspin functions as a critical regulator that alleviates pathological cardiac hypertrophy by regulating regulator that alleviates pathological cardiac hypertrophy by regulating autophagyautophagy--ddependent myocardial senescence, which provides potential preventive and ependent myocardial senescence, which provides potential preventive and therapeutic targets for pathological cardiac hypertrophy.therapeutic targets for pathological cardiac hypertrophy.
Microbiota Thrombus Colonization May Influence Athero-ThrombosIs in Hyperglycemic Patients With ST Segment Elevation Myocardial Infarction (STEMI). MARIANELLA Study
Authors: Sardu Celestino , Trotta Maria Consiglia , Santella Biagio , Onofrio Nunzia D' , Barbieri Michelangela , Rizzo Maria Rosaria , Sasso Ferdinando Carlo , Scisciola Lucia , Turriziani Fabrizio , Torella Michele , Portoghese Michele , Loreni Francesco , Mureddu Simone , Lepore Maria Antonietta , Galdiero Massimiliano , Franci Gianluigi , Folliero Veronica , Petrillo Arianna , Boatti Lara , Minicucci Fabio , Mauro Ciro , Calabrò Paolo , Feo Marisa De , Balestrieri Maria Luisa , Ercolini Danilo , Amico Michele D' , Paolisso Giuseppe , Galdiero Marilena , Marfellla Raffaele 
Published: November 2020

Abstract
Objectives. We examined the association of the coronary thrombus microbiota and relative metabolites with major adverse cardiovascular events (MACE) in hyperglycemic patients with ST segment elevation myocardial infarction (STEMI).Background. Hyperglycemia during STEMI may affect both development and progression of coronary thrombus via gut and thrombus microbiota modifications. Methods. We undertook an observational cohort study of 146 first STEMI patients treated with primary percutaneous coronary intervention (PPCI) and thrombus-aspiration (TA). Patients were clustered, based on admission blood glucose levels, in hyperglycemic (>140 mg/dl) and normoglycemic (<140 mg/dl). We analyzed gut and thrombus microbiota in all patients. Moreover, we assessed TMAO, CD40L and von Willebrand Factor (vWF) in coronary thrombi. Cox regressions were used for the association between Prevotellaspp and TMAO terziles and MACE. MACE endpoint at 1 year included death, re-infarction, unstable angina.Results. In fecal and thrombus samples, we observed a significantly different prevalence of both Prevotellaspp and Alistipesspp. between patients with hyperglycemia (n=56) and those with normal glucose levels (n=90). The abundance of Prevotella increased in hyperglycemic vs normoglycemic patients whereas the contrary was observed for Alistipes. Interestingly, in coronary thrombus, the content of Prevotella was associated with admission blood glucose levels (p<0.01), thrombus dimensions (p<0.01), TMAO, CDL40 (p<0.01) and vWF (p<0.01) coronary thrombus contents. Multivariate Cox-analysis disclosed a reduced survival in patients with high levels of Prevotella and TMAO in coronary thrombus as compared to patients with low levels of Prevotella and TMAO, after 1-year follow up.Conclusions. Hyperglycemia during STEMI may increase coronary thrombus burden via gut and thrombus microbiota dysbiosis characterized by an increase of Prevotella and TMAO content in thrombi.
Vitamin D Low-Levels and Silent Myocardial Ischemia in Type 2 Diabetes: Clinical Correlations and Prognostic Significance
Authors: Rossi Rosario , Talarico Marisa , Rolando Cristina , Minici Roberto , Boriani Giuseppe 
Published: November 2020

Abstract
BACKGROUND: Vitamin D deficiency has a pathogenetic and prognostic role in coronary artery disease. Moreover, Vitamin D has a key role in pain modulation and transmission. Diabetic patients have a high risk of silent myocardial ischemia (SMI), due to diabetic neuropathy. We aimed to evaluate the correlation between SMI and Vitamin D serum levels in a population of type 2 diabetic patients and to assess whether SMI patients had a worse survival than their symptomatic counterpart.METHODS: In this retrospective, cross-sectional, observational study, we enrolled 253 patients admitted in Policlinico of Modena Hospital and compared them with 50 healthy volunteers. We divided the 253 patients into three sub-groups: symptomatic MI group (125, 32.4%); SMI (78, 25.7%), and no-MI one (50, 41.9%). The entire population had a 25-hydroxyvitamin D (25(OH)D) measurement. RESULTS: 25(OH)D levels (nmol/l) were lower in SMI group (34.9 ± 5.8) respect to symptomatic MI (49.6 ± 6.1; p = 0.01); no MI (53.1 ± 6.2; p = 0.001) and control group (62.1 ± 6.7; p = 0.0001). 25(OH)D level was the only independent variable able to influence the development of SMI in diabetic patients, with an inverted odd-ratio of 1.11 (p = 0.01). Symptomatic MI group had better survival than SMI one (6-year survival rate: 83 vs. 69%; p = 0.01)CONCLUSIONS: Type 2 diabetic with SMI had a higher mortality risk and showed lower 25(OH)D levels than the symptomatic group. This suggests the crucial vitamin D role in the pathogenesis of SMI.
The Direct Effects of Different Irradiation Methods on The Survival of Laryngeal Squamous Carcinoma Hep2 Cells
Authors: Tian Suqing , Huang Li , Wang Junjie 
Published: November 2020

Abstract
Radiotherapy plays an important role in the treatment of laryngeal squamous cell carcinoma. However, radiation resistance is an important cause of radiotherapy failure. To determine the direct effects of different irradiation methods on the survival of Hep2 cells, three different irradiation methods were used in vitro to explore the effects on the colony formation, cycle and apoptosis, showing that different irradiation methods had inhibitory effects on the colony formation of Hep2 cells. Radioactive 125I continuous low dose rate radiation (CLDR) has a stronger inhibitory function, and single dose radiation (SDR) is also superior to fractionated dose radiation (FDR). The G1 phase decreased more significantly, while the G2/M phase ratio also increased significantly in CLDR group. The CLDR group was more significant increase in the early apoptosis and general apoptosis. During these process, the production of higher γ-H2AX, Cyclin D1, Cdc2, NF-κB p21, CyclinB1 and lower p-Cdc25c in CLDR group were observed. Taken together, the continuous low-dose rate irradiation of 125I seeds can significantly increase the apoptosis rate of Hep2 cells, resulting in continuous G2/M phase arrest and significantly inhibiting the proliferation ability of Hep2 cells
Shen-Qi-Wan Protects the Renal Peritubular Capillary From Adenine-mediated Damage by Upregulating Aquaporin 1
Authors: Bao Yuting , Zhang Yehui , Yang Yuanxiao , Chen Xueming , Lin Luning , Fu Yunbo , Ji Liting , Li Changyu 
Published: November 2020

Abstract
Background：Shen-Qi-Wan (SQW), a commonly used prescription against chronic kidney disease (CKD) in Traditional Chinese Medicine (TCM), has a nephroprotective action in adenine-induced kidney injury. However, the mechanism of SQW in renal injury remains unclear.Methods: We evaluated the nephroprotective action of SQW in adenine-induced kidney injury and investigated its mechanism in vitro studies.Results: SQW supplementation could alleviate the pathological makers for CKD, ameliorate dysfunction of Hypothalamic-Pituitary-Adrenal (HPA) axis and renal function loss caused by adenine. Alternatively, SQW administration showed an ameliorating effect from the toxicity and alleviated the injury of capillaries around renal tubules instigated by adenine through increasing AQP1 mRNA and protein level. In vitro experiments, SQW medicated serum enhanced the migration and lumen formation ability of HMEC-1 cells, and significantly increased AQP1 protein level. Moreover, AQP1 knockdown efficiently inhibited migration and lumen formation ability in HMEC-1 cells which could be reversed by SQW medicated serum.Trial registration : This is an animal and cell experiment, trial registration is not necessary.Conclusion: These results suggested that SQW attenuated peritubular capillary injury in adenine induced CKD model rats through boosting angiogenesis in endothelial cell and AQP1 may be a potential target of SQW for treating renal injury.
Integration Traditional Chinese Medicine and Nibble Debridement and Dressing Method Reduce Thrombosis and Inflammatory Response in the Treatment of Thromboangiitis Obliterans
Authors: Li Jianhua , Zhong Jingfeng , Huang Chunfa , Guo Jiewen , Li Arong 
Published: November 2020

Abstract
Background: Thromboangiitis obliterans (TAO), also known as Buerger's disease, is an occlusive arterial disease. However, the pathogenesis of TAO is still unclear. Research has shown that traditional Chinese medicine (TCM) has significant advantages in the treatment of TAO. Our purpose is to explore the underlying roles of TCM in combination with nibble debridement and dressing method (NDDM) in TAO rat model.Methods: 10 mg/ml sodium laurate was utilized to establish a TAO rat model, and then the TAO mode rats were treated with notoginseng powder (NP), maifusheng (MFS) or the combination of NP or MFS and NDDM. Ganrene’s classification and, blood rheology was evaluated; the pathological characteristics of rat limbs were examined by H&E staining and Masson staining; CD3+ and CD20+ levels were measured by immunohistochemistry and flow cytometry. In addition, inflammation-associated cytokines were analyzed by RT-qPCR, Western blot and ELISA assays. Results: ntegration NP or MFS and NDDM dramatically reduced the ganrene’s classification and affected blood rheology parameter of TAO model rats compared with NP and MFS alone. Meanwhile, NP or MFS in combination with NDDM decreased CD3+CD20+T cells, reduced thrombosis and inflammatory cell infiltration, and dramatically decreased the levels of inflammation-associated cytokines.Conclusion: Our results suggested that integration NP or MFS and NDDM could relieve the symptoms of TAO model rats induced by sodium laurate, which might provide new management strategy for TAO.