bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Real-Time Vital Sign Monitor for Heart Rate and SPO2 Parameter Using Internet of Things Technology
Authors: Utomo Bedjo , Triwiyanto  , Luthfiyah Sari , Mudjiono Urip , Hamzah Torib 
Published: November 2019

Abstract
Recently, the internet of thing (IOT) has been growing rapidly in many fields including in the biomedical vital sign monitoring of a human. Heart rate and SPO2 are vital sign parameters for human life. Those parameters needed to be observed for patients in an emergency situation. In patient care at hospitals, doctors need to monitor the patients’ heart rate and SPO2 parameters continuously. Therefore, the objective of this study was to develop the heart rate and SPO2 parameters based on IOT technology. The heart rate and SPO2 parameters were detected using a finger sensor and an oximeter sensor, respectively. The digital data from those sensors, further, was processed using ESP8266 microcontroller. By using a built-in Wi-Fi module in ESP8266, the vital sign was sent to the user wirelessly to handphone or central monitor using Think Speak application, remotely. In this experiment, the sensors were placed on the finger while the patient was in the standing, lying or sitting position. In the same position, the parameters were acquired for ten times. Some analyses were conducted to find the accuracy of the measurement. The results showed that the uncertainty were 0.26 and 0.04 for heart rate and SPO2, respectively. Based on the medical standard calibration (<3%), those parameters are feasible to be used in the medical system measurement. The T-test analysis statistics for one independent sample was performed to obtain a significant difference among the data. The p-values were 0.118 and 0.000 for heart rate and SpO2, respectively. Finally, this study suggested that the IOT system was able to monitor the parameters effectively.
Application of Intelligent Search Algorithms in Proportional-Integral-Derivative Control of Direct-Current Motor System
Authors: Ma’arif Alfian , Nabila Hanamirza , Iswanto  , Wahyunggoro Oyas 
Published: November 2019

Abstract
Proportional-Integral-Derivative (PID) control is one of the famous controllers applied in industrial and robotics world. It is quite easy to understand and to be implemented. It is known that, with a proper combination of its gain parameters, a system with good performance can be obtained. This parameter tuning can be tricky since the number of the combination is almost infinite. Hence, methods to tune the parameter gains of proportional, integral and derivative is needed. Some optimization methods that can be used in tuning parameters are Genetic Algorithm (GA), Particle Swarm Optimization (PSO) and Differential Evolution (DE). The paper will analyse the performance of those methods by applying them in PID Controller to control a Direct Current (DC) motor system by comparing the iteration time, the number of parameters, and augmented system performance. The comparison is done on MATLAB simulation by using the same computer. It results as DE as the best among them with the least number of parameters and best DC motor system performances.
Adaptive Trajectory Tracking Control System of Two-Wheeled Robot
Authors: Uddin Nur 
Published: November 2019

Abstract
A design of two-wheeled robot (TWR) trajectory tracking control system (TTCS) using model reference adaptive control method is presented. The TTCS is to steer the TWR track a desired trajectory. The tracking system dynamic is represented by a posture error dynamic. The posture error dynamic is derived based on the robot kinematics. Assuming small heading angles the posture error dynamic is approached by a a linear system. Model reference adaptive control (MRAC) is applied in designing the TTCS based on the linear posture error dynamic. The TTCS performace is evaluated through computer simulation. The simulation results show that the designed controller is able to make the TWR track a desired trajectory.
The Effect of Snake Fruit (Salacca edulis Reinw.) Seed Extract On Body Weight In Female Rats Model with Iron Deficiency Anemia
Authors: Melati D , Tamtomo D G , Indarto D 
Published: November 2019

Abstract
Anemia is a global nutritional problem in developing countries. Oral iron supplementation is recommended for anemia treatment in pregnant woman, and adolescent girl. However, this supplementation frequently has some side effects. In a previous study, snake fruit seeds naturally contain high level of iron, which is potentially used for alternative anemia supplementation. This study aimed to investigate the effect of snake fruit seed extract (SSE) on body weight (BW) in female rat model with anemia. The study was a pre-posttests control group design, 14 female rats strain Wistar were selected based on inclusion criteria. After 10 days modelling of anemia, all rats were randomly divided to 2 groups: control and treatment groups. For the remaining days, all rats received low iron feed, but added 1.75 g/kg BW/day SSE in the T group 14 days treatment. Body weight was measured before and after treatment, and all data were analyzed using independent and paired T-tests with p<0.05. Before treatment, the average of BW in the C group is lower than T group (p=0.005). After treatment, the average of BW in T and C groups were statistically differed from BW before treatment (p<0.001). In addition, a greater number of BW was found in the T group, compared with the C group (p<0.001). The administration of 0.175 g/100 g BW/day SSE can increase BW in female rats model with anemia.
Effect of Active Compound of Pasak Bumi Root (Euricoma longifolia, Jack) as an inhibitor of CDK2 methylation: In Silico Study
Authors: Rahman E Y , Ali M , Purnomo B B , Kania N , Utomo D H , Nurikhwan P W 
Published: November 2019

Abstract
Active compound of Pasak Bumi’s root, a native plant of Indonesia, is used as antitumor by triggering cell apoptosis and reactivating the silence tumor suppressor gene caused by hypermethylation, inhibiting cancer cell proliferation. The aim of this study is to know the role of quassinoid from Pasak Bumi’s root (Eurycoma longifolia Jack) as an inhibitor of CDK2 methylation in silico. This is a descriptive study. CDK2 samples are obtained from Protein Data Bank (RSCB.org) with ID3SWR, samples of natural quassinoid are obtained from PUBCHEM NCBI and controlled by Sunitinib (®Sutent). Autodock Vina program PyRx 0.8 is used to analyze Molecular Docking. The process of analyzing molecular interactions is carried out using the LigandScout V.2.0 program. Visualization process are carried out using LigandScout V.2.0 program. The affinity of quassinoid and sutinab to CDK are -6.1 and -9.4, respectively. The more negative the binding affinity value, the better the ability of the compound (ligand) to bind to the receptor (macromolecules). From this case, Sutinab has better value compared to quassinoid. Target protein analysis using HITPICK shows quassinoid’s target predictor is JUN protein. Protein interaction analysis are obtained, and the compound is using stitch. JUN protein and Sunitib could bind with CDK2. The conclusion of this study is Sutinib has greater affinity compared to quassinoid.
Hepatoprotective effect of Eugenol on Acetaminophen-Induced Hepatotoxicity in HepG2 cells
Authors: Lister I N E , Ginting C N , Girsang E , Amansyah A , Chiuman L , Yanti N L W E , Rizal R , Widowati W 
Published: November 2019

Abstract
Imbalance in liver metabolism lead to oxidative stress mainly caused by free radicals or termed as reactive oxidative oxygen (ROS). Prolonged ROS exposure without proper treatment induce severe liver damage and serious hepatic diseases including cirrhosis. Eugenol (4-allyl 2-methoxyphenol) is phenolic derivative compound that showed antioxidant, anti-inflammatory, analgesic, antibacterial, antifungal, and antitumor activities. This study aims to evaluate the hepatoprotective effect of eugenol through biochemical markers analysis. Cytotoxic assay was performed in various concentrations of eugenol (3,125; 6,25; 12,5; 25; 50; 100 μg/mL) using (3-(4,5-dimethylthiazol-2yl)-5-(3-carboxymethoxyphenkyl)-2-(4-sulfophenyl)-2H-tetrazolium) to determine the safe concentrations for next assays. Aspartate aminotransferase (AST), alanin aminotransferase (ALT), and lactate dehydrogenase (LDH) assay were performed using colorimetric method to evaluate the levels and activity of liver-related enzymes which are elevated in damaged liver as they were used as hepatotoxicity markers. The viability of HepG2 cells increased in eugenol concentration 3.125 μg/mL and then decreased along with the rise of eugenol concentrations. From this cytotoxic assay, two concentrations of eugenol were choosen (6.25 and 25 ug/ml) to be evaluated in the next assays. The level of LDH, ALT, and AST decreased after eugenol treatment compared to negative control. The most effective concentration of eugenol to seemed different in certain hepatotoxicity markers. This study suggests that eugenol was safe to use for cells culture environment in large ranges of concentrations and shows hepatoprotective effect in APAP-induced hepatotoxicity model by the decrease of LDH level and AST and ALT activities.
DNA barcoding as a method for species identification: case study in Ahaetulla snake
Authors: Indahsari L I N , Fatchiyah F , Kurniawan N 
Published: November 2019

AbstractSpecies identification using molecular analyses has recently been developed, one of which is DNA barcoding method. The purpose of this study was to apply the DNA barcoding method to identifyAhaetullasnake which previously had not been identified molecularly. The specimens used came from Sumatra, Java, Bali, Lombok and additional samples obtained from Genbank. Cytochrome B gene was extracted and amplified to obtain DNA barcode. Phylogenetic analysis using ML method was used to determine the kinship relationship of theAhaetullasnake. Morphological analyses were added to confirm the results of molecular identification. The results of molecular analysis using DNA barcoding method showed the presence of twoAhaetullaspecies in this study, there areA. prasinaandA. mycterizans.This difference is indicated by the formation of two separate main clades, clade I containsA. prasinaand clade II containsA. mycterizans.The results of the morphological analyses also showed differences in the character of the two different clade species. Thus, molecular analyses result agrees with morphological analyses. It can be concluded that the DNA barcoding method can be used as a valid method to identifyAhaetullasnake species.
Antioxidant potential ethanolic extract of Glycine max (l.) Merr. Var. Detam and daidzein
Authors: Prahastuti S , Hidayat M , Hasianna S T , Widowati W , Amalia A , Yusepany D T , Rizal R , Kusuma H S W 
Published: November 2019

AbstractAntioxidants in chemical terms are electron donors and antioxidants are biologically compounds that can overcome the negative effects of oxidants in the body such as damage to vital cells of the body. The balance between oxidants and antioxidants is very important. Antioxidant from natural sources are safer than synthetic antioxidants. Soybeans are functional food source that contains essential amino acids, vitamin e, saponins and are rich in antioxidants such as flavonoids, isoflavones and anthocyanins. The aim of this research is to investigate antioxidant potential of ethanolic extract of black soybean (EEBS) and its compound daidzein. The phytochemical screening assay evaluated by modified Farnsworth methods and to find out phenolic and flavonoid content, total phenols and flavonoids were tested. DPPH (2,2-diphenyl 1-pichylhydazy), ABTS (2,2’-Azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) reducing activity, FRAP (ferric reducing antioxidant power) activity and H2O2 scavenging activity assay were used to measure antioxidant activity. The analysis of phytochemical of EEBS exhibit the presence of saponins, alkaloids, tannins, steroids and triterpenoids and terpenoids. Total phenol and flavonoid assay showed the presence of phenols and flavonoids. Four assay of antioxidant activity showed that EEBS at highest concentration exhibit higher activity (%), and from three assays EEBS has higher antioxidant activity (expressed as IC50) than daidzein. Overall, three of the four antioxidant tests performed can be concluded that EEBS has better antioxidants activity than daidzein. Further research is needed regarding black soybean as a promising antioxidant resource.
Cellulose nanocrystal (CNC) produced from the sulphuric acid hydrolysis of the pine cone flower waste (Pinus merkusii Jungh Et De Vriese)
Authors: Lusiana S E , Srihardyastutie A , Masruri M 
Published: November 2019

Abstract
Cellulose nanocrystal (CNC) has been produced up on the hydrolysis of the cellulose isolated from the waste of pine flower (Pinus merkusii Jungh Et De Vriese). The hydrolysis process is undergone using 10%, 30% and 60% of sulfuric acid solution. the hydrolysis process is also performed using normal heating at 70°C under stirring with magnetic stirrer for 4 hours. The CNC afforded is characterized by means of infra red spectrophotometry (FTIR), x-ray diffraction spectrometry (XRD) and transmission electron microscope (TEM) analysis. The TEM analysis afford the dimension of CNC in about 15 nm × 185 nm. This finding open the way for further prospective application.
Comparative Analysis of Wharton’s Jelly Mesenchymal Stem Cell (WJ-MSCs) Isolated Using Explant and Enzymatic Methods
Authors: Widowati W , Gunanegara R F , Rizal R , Widodo W S , Amalia A , Wibowo S H B , Handono K , Marlina M , Lister I N E , Chiuman L 
Published: November 2019

Abstract
Wharton’s Jelly is one of the best sources for mesenchymal stem cells. Human Wharton’s Jelly Mesenchymal Stem Cells (hWJ-MSCs) have high proliferation, multi-lineage differentiation potential, and do not produce any teratogen or carcinogen. These characteristics make hWJ-MSCs become suitable for regenerative medicine. Some methods were developed to isolate hWJ-MSCs from umbilical cord, such as explant method and enzymatic method. This study aims to characterize hWJ-MSCs which are isolated by two different methods, explant attachment method and enzymatic method. hWJ-MSCs isolation was performed through explant method and enzymatic method using trypsin, hyaluronidase and collagenase type 1 with certain ratio of concentration. Isolated hWJ-MSCs was characterized using flow cytometer to detect the expression of CD44, CD90, CD105, CD73 and negative lineage. MSCs differentiation assay was performed to analyze adipogenic, chondrogenic and osteogenic cells lineage. We successfully isolated hWJ-MSCs from umbilical cord through enzymatic and explant methods. Immunophenotyping assay through flow cytometry analysis showed high purity of WJ-MSCs. The isolated hWJ-MSCs from both methods showed positive expression of CD44, CD90, CD105, and CD73. The isolated hWJ-MSCs exhibited capacity to differentiate into adipocyte, chondrocyte, and osteocyte cells. hWJ-MSCs isolated through explant and enzymatic method have high proliferation capacity and be able to differentiate into three different lineage cells. Both methods explant attachment and enzymatic methods are efficiently produced hWJ-MSCs.
In Silico: γ-Oryzanol as Anti-inflammatory During Folliculogenesis in Rattus Novergicus Exposed to Pyrethroid Aerosol
Authors: Lisnawati L , Santoso M I E , Poeranto S , Endharti A T 
Published: November 2019

Abstract
The use of insect repellent is a disease preventive effort due to mosquito vectors as well as sources of pollutants that disrupt human health, especially reproductive health. This study aimed to determine the effect of γ-Oryzanol on the expression of Foxo3a, TNF-α, GDF-9 in the folliculogenesis of mice (Rattus Novergicus) exposed to pyrethroid aerosol mosquitoes. The research use bioinformatics technique with in silico approach. The result of study showing that γ-oryzanol has the potential as an anti-inflammatory and NO scavenger, the influence of the active compound γ-oryzanol on Foxo3 expression obtained pathway analysis with the results of the prediction of the target HITPICK able to bind to APP, AKT1 and Mtor expressions through modification of post-translational Foxo1, Effect of γ-oryzanol on Gdf9 expression, through bonding with APP, AKT1, AKT3, Pik3cg and modifying Smad 4 post-translation, The effect of the active compound γ-oryzanol on TNF-α expression via APP binds with AKT1, AKT3, AKT2 then activates IkbkB and bindings with Casp3, Fadd, Tradd, Tnfrsf1a, Tnfrsf1b, Traf3, Birc2, Ikbkg. Based on average part length analysis AKT1 is the fastest interact with protein target. The conclusion, γ-oryzanol has predicted to be used for the treatment of insecticide exposure, because it can act as an anti-inflamatory, and lipid perosidase inhibitor. This condition influences normally folliculogenesis with stable expression of Foxo3a, GDF 9 and TNF-α.
Urological cancer: molecular docking of the active compound Scurrula atropurpurea against nuclear factor erythroid2-related factor2 (Nrf2)
Authors: Prasetya H , Dewi F R P , Setiawan B 
Published: November 2019

Abstract
The nuclear factor erythroid2-related factor2 (Nrf2) is a transcription factor for redox homeostasis involved in antioxidant genes and detoxification enzymes. This transcription factor provides protection against organs and is involved in urological cancer progression. This study aims to investigate the interaction between the active compounds of Scurrula atropurpurea against the Nrf2 signal. This study was an in silico study. The research protocol consisted of searching for amino acids making up the Nrf2-Keap1 system, searching for the structure of the active component of Scurrula atropurpurea, modeling 3D protein structures, docking and visualization between protein-ligand, and analyzing bond interactions between proteins and ligands. The active compounds of Scurrula atropurpurea which are molecularly docking include aviculin, caffeine, catechin, epicatechin, kaempferol, quercetin, quercitrin, rutin, and theobromine. For interactions with Nrf2, rutin was easier to interact compared to other compounds. Energy interactions between caffeine, catechin, kaempferol, quercetin, quercitrin, and rutin were lower than the energy of interaction between Nrf2 and Keap-1. It was concluded that some of the active compounds of Scurrula atropurpurea can modulate the Nrf2 signal. Thus, there is an active compound from Scurrula atropurpurea which can be an anticancer urological candidate via an Nrf2 signal.
Estimation of leaf antioxidant activity using image processing
Authors: Suhartono E , Setiawan B , Santosa P B , Idroes R , Indrawan M S 
Published: November 2019

Abstract
Physiologically, the influence of inundated conditions on many plants varies. The eco-physiological response of most plantations to flooding is wilt and stomata closure, root damage, reduction of root biomass2. This results in a decrease in the production of bioactive compounds, which have an effect on decreasing antioxidant activity. Various methods of measuring antioxidant activity still use chemicals through extraction processes that require a long time. So, it is necessary to examine alternative methods that are cheap, easy and fast. This method is digital processing. The leaf samples were then photographed for image analysis in the Telkom School Banjarbaru Multimedia Laboratory. Antioxidant activity was analyzed through the hydroxyl scavenging method at the laboratory of Biochemistry and Biomolecular, Faculty of Medicine, Lambung Mangkurat University, Banjarbaru. The results of image analysis showed that the antioxidant activity model of RGB values was AA= -54.9592 +1.0295 R + 0.3757 G + 2,006 B -0,0004 R*G - 0.0012 R*B + 0.004 G*B (AA = antioxidant activity; R = red; G = green; B = blue) with p = 0.001 (p <0.005) and r = 0.76. In this model, the R value has a significant effect on antioxidant activity. Image analysis using RGB can be used to measure leaf antioxidant activity. However, further research is needed.
Development potential of beef cattle business and alternative strategies development of beef cattle in Pamekasan
Authors: Riszqina R , Nurlaila S , Suparno S , Heryadi A Y 
Published: November 2019

Abstract
This study aims to (1) determine the potential development of beef cattle business based on the availability of natural resources and human resources in Pamekasan; (2) to determine alternative-strategy of beef cattle development in accordance with Kabupaten Pamekasan. This research uses descriptive analysis, LQ analysis, KPPTR analysis and SWOT analysis. This study was conducted from July 15, 2017, to July 31, 2017, using survey method, to obtain primary data by using 60 respondents from 4 sub-districts selected purposively and 2 stakeholders in Kabupaten Pamekasan. The data used consist of primary data and secondary data. Primary data is obtained directly from interviews with farmers and related parties. Secondary data obtained from the Department of Animal Husbandry, Department of Agriculture and Central Statistics Agency (BPS) Pamekasan District. the results showed that (1) the potential area of the development of beef cattle farming based on the availability of natural resources in Kabupaten Pamekasan based on LQ> 1 value ranging from 1.0129 to 1.3889 obtained from Tlanakan, Pademawu, Pamekasan, Palengaan, Pakong, Waru, Batumarmar and Pasean, as the base area of livestock development with maximum natural resources potential of 221,252 ha, and maximum potential of livestock equal to 152,121,1 AU (2) The results of analysis of policy strategies that can be suggested for the development of beef cattle in Kabupaten Pamekasan are: (a) To optimize the government program (UPSUS SIWAB) in producing calves of beef cattle (b) Providing education and training programs to farmers in the field (c) Exploiting unproductive land for cattle ranching (d) Strengthening farmer institutions so that farmers have strong bargaining power (e) Utilizing livestock markets to increase the selling and demand of beef cattle.
Cleaning schedule for increased energy efficiency on heat exchanger process: Sugar plant case study
Authors: Sudarni D H A 
Published: November 2019

Abstract
THE process in a plant, which has had integration heat for energy recovery, fouling or the formation of a precipitate on a heat exchanger (heat an exchanger) often pose a problem in the production of operational. As increase energy consumption so as to cause economic loss. Loss can be reduced if it is undertaken cleansing routinely. Next up is how problems to timetable cleansing instrument an exchanger heat exchanger (heat in a complex in an industry that efficient). A schedule cleaning in heat may be based on knowledge about the behaviour of fouling how fast the thermal resistance. In general research will be conducted to use a calculation model of mathematics for scheduling optimizations cleaning on heat exchanger network (HENs and it can be applied in the chemical industry. Fouling increase to the function of time, in this study model mathematics developed to determine many the act of cleaning and time where the money to be cleared industry that operational can optimally.