bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficient anchoring of Erianthus arundinaceus chromatin introgressed into sugarcane by specific molecular markers
Authors: Huang Yongji , Wu Jiayun , Li Xueting , Yu Fan , Hu Xuguang , Luo Ling , Zhang Muqing , Xu Liangnian , Deng Zuhu 
Published: February 2020

Abstract
Erianthus arundinaceus is a valuable gene reservoir for sugarcane improvement. However, insufficient molecular markers for high-accuracy identification and tracking of the introgression status of E. arundinaceus chromatin impede sugarcane breeding. Fortunately, suppression subtractive hybridization (SSH) technology provides an excellent opportunity for development of high-throughput E. arundinaceus-specific molecular markers at a reasonable cost. In this study, we constructed a SSH library of E. arundinaceus. In total, 288 clones E. arundinaceus-specific repetitive sequences were screened out and their distribution patterns on chromosomes were characterized by fluorescence in situ hybridization (FISH). A subtelomeric repetitive sequence Ea086 and a diffusive repetitive sequence Ea009, plus 45S rDNA-bearing E. arundinaceus chromosome repetitive sequence EaITS were developed as E. arundinaceus-specific molecular markers, namely Ea086-128, Ea009-257, and EaITS-278, covering all the E. arundinaceus chromosomes for high-accuracy identification of putative progeny. Both Ea086-128 and Ea009-257 were successfully applied to identify the authenticity of F1, BC1, BC2, BC3, and BC4 progeny between sugarcane and E. arundinaceus. In addition, EaITS-278 was a 45S rDNA-bearing E. arundinaceus chromosome-specific molecular marker for rapid tracking the inherited status of this chromosome in sugarcane background. Three BC3 progeny had apparently lost the 45S rDNA-bearing E. arundinaceus chromosome. We reported herein a highly effective and reliable SSH-based technology for discovery of high-throughput E. arundinaceus-specific sequences bearing high potential as molecular markers. Given its reliability and savings in time and efforts, the method is also suitable for development of species-specific molecular markers for other important wild relatives to accelerate introgression of wild relatives into sugarcane.
Annexin A2 Promotes Development of Retinal Neovascularization through PI3K/ AKT Pathway
Authors: Li Chen-yue , Zhao Zi-chang , Zhao Shi-hong 
Published: February 2020

Abstract
Background RNV is a pathological characteristic of PDR and ANXA2 play an important role in the process of RNV while the mechanism remains unclear. We explore the role and molecular basis of ANXA2 in the formation of RNV and seek for new potential targets for the prevention and treatment of PDR. Methods Lentivirus containing plasmids which can interfere ANXA2 and overexpress ANXA2 were packaged and infected HRECs, dividing HRECs into 4 groups. Moreover, 1ul SC79 solution was added in shA2 group and 1ul LY294002 solution was added into lentiA2 group. Western Blot was used to detect expression of ANXA2 and changes in phosphorylation degree of major proteins in PI3K/AKT signaling pathway in each group of HRECs. HRECs of all groups were used to perform EDu cell proliferation assay, Transwell cell migration assay and Matrix tube formation assay. C57BL/6J mice were randomly divided into 3 groups. Mice of OIR group were injected intraperitoneally with 0.05ml PBS every day from 7th to 10th day while mice of LY294002 treatment group were injected with 0.05ml LY294002 solution and no treatment in the control group. Lectin GS-IB4 fluorescence staining was used to observe RNV in mice in all groups. The expression of ANXA2 in mouse retinas was detected by Westen-blot. Results The proliferation, immigration and angiogenesis ability of HRECs is lower in shA2 group than shNC and SC79 treatment group while higher in lentiA2 group than lenti-EGFP and LY294002 treatment group. ANXA2 expression is significantly higher in retina of mice in OIR group and LY294002 treatment group. RNV is significantly less severe in LY294002 treatment group than that in OIR group. Conclusions ANXA2 can promote development of RNV through PI3K/ AKT Pathway.
Improving Evaluation of Potato Resistance to Rhizoctonia solani infection by Optimizing Inoculum Method Combined with Toxin-based Assay
Authors: Zhang Xiaoyu , Xing Xing , Feng Yayan , Yu Zhuo , Hao Jianjun , Li Dezhou 
Published: February 2020

Abstract
Background: Rhizoctonia solani causes stem canker and significantly impacts the production of potato. Conventional assay to evaluate potato resistance using R. solani inoculum is time consuming. To establish an effective and fast assay, 20 potato germplasms were examined using both R. solani inoculum and R. solani-derived toxin (RS toxin). Results: In field trials of 2009 and 2010, wheat-bran-based inoculum of R. solani was incorporated at either 0, 2, 3, 4 or 5 g per seed piece in the soil followed by sowing potato seed pieces in the furrow. Stem canker was evaluated in the growing season. Inoculum of wheat-bran-based 2, 3, or 4 g could well distinguish resistance of potato germplasms. For a quick assay of resistance screening, a toxin-based method was established by treating potato seedlings with the toxin of R. solani (RS toxin). RS toxin was prepared by heating R. solani culture. Potato seedlings were obtained through tissue culture and grown in Murashige and Skoog medium. Seedlings at the stage of 12 cm in height were transferred into agar medium amended with RS toxin and incubated for eight days. The inhibition caused by RS toxin was positively correlated with toxin concentration. By evaluating various potato cultivars that have different sensitivities to toxin, the inhibition of potato stems sections and seedlings was from 33% to 100% and from 32% to 148%, respectively. Results of toxin-based evaluation were highly correlated with the field data using pathogen inoculum (r = 0.731, P < 0.01). Conclusions: Inoculation with wheat bran-mediated R. solani of 2, 3 or 4 g per seed piece was an effective method for the evaluation of potato resistance in field trials. The toxin-based assay could improve efficiency and speed of disease resistance evaluation of potato germplasms. Both assays showed that none of the 20 potato materials was completely resistant to R. solani. However, cultivar ‘Desiree’ had the lowest level of disease, whereas ‘Atlantic’, ‘Favorita’, and ‘Shepody’ showed the high susceptibility.
Body mass index in relation to prostate specific antigen-related parameters
Authors: Lin Dandan , Liu Ting , Chen Luling , Chen Zongtao 
Published: February 2020

Abstract
Purpose Limit previous studies were conducted to assess the association between body mass index (BMI) and series of prostate-specific antigen (PSA)-related parameters, which taken prostate volume (PV) and blood volume (BV) into consideration. The objective of this study was to assess the relationship between BMI and parameters of PSA concentrations in Chinese men. Methods A total of 86,930 men who had undergone annual medical examinations at the First Affiliated Hospital of Army Medical University between 1 January 2011 and 31 December 2018 were included. Partial Spearman correlation rank test was used to assess the relationship between BMI, PV, BV and PSA, and then estimated the correlation between BMI and series of PSA-related parameters. Results After adjustment for age, PV (correlation coefficient = 0.227, P-value < 0.001) was positively associated with PSA levels, but BMI (correlation coefficient = -0.057, P-value < 0.001) and BV (correlation coefficient = -0.041, P-value < 0.001) inversely correlated to PSA concentrations. Moreover, present study also indicated that BMI positively associated with PV and BV. Furthermore, present study identified that PSA mass (correlation coefficient = 0.001, P-value = 0.763) was not affected by obesity after adjustment for age in Chinese general men.Conclusion The results of this large-sample, hospital-based study in China indicated that a higher BMI might have a bigger PV and larger BV. BMI was negatively associated with PSA and PAS density, and no significantly association was detect between BMI and PSA mass.
Low expression of plakoglobin in intratumoral liver tissue is associated with poor survival after curative resection of Hepatocellular Carcinoma
Authors: Ma De-Ning , Liu Zhuo , Yuan Jie , Gao Xin-Yi , Sun Dian-Dian , Fu Zhi-Xuan 
Published: February 2020

Abstract
Background: Plakoglobin also known as γ-catenin, is a member of the Armadillo family. It’s a structural and functional homologue of protein β-catenin with dual functions in cell-cell adhesion and cell signaling. However, the role of plakoglobin in hepatocellular carcinoma (HCC) is unclear to date. Our aim was to investigate the expression of plakoglobin in HCC and its correlation with prognosis and then to further study the underlying function.Methods: Western Blot and qRT-PCR were used to examine the expression of plakoglobin in HCC cell lines and normal hepatocyte cell line. Immunohistochemical method was used to analyze the expression and relationship of plakoglobin in tissue microarray with 191 HCC patients through Kaplan-Meier analysis.Results: Patients with high expression of plakoglobin had a better survival compared with those with low expression (OS, P=0.002; DFS, P=0.012). Plakoglobin is an independent prognostic factor for patients (OS, P=0.026, DFS, P=0.045). Plakoglobin was inversely consistent with the metastatic potential ability of HCC cell lines.Conclusions: Our founding indicates plakoglobin is an independent prognostic marker of HCC, and patients with low expression of that had a poor survival. Plakoglobin may serve as a potential target for anti-HCC.
Recruitment, experience, and retention among women with HIV and hazardous drinking enrolled in the WHAT-IF? Study
Authors: Canidate Shantrel , Cook Christa , Varma Deepthi , Carnaby Giselle , Ennis Nicole , Stetten Nichole , Cook Robert 
Published: February 2020

Abstract
Background: Despite efforts by the NIH to enhance the participation of women and minorities in clinical research, women with or without HIV continue to remain underrepresented. The purpose of this study is to better understand the reasons why women with HIV and hazardous drinking participated in the WHAT-IF? Study and to discuss their experience (positive or negative) in the study. Methods: Convenience and theoretical sampling were used to recruit women with HIV and hazardous drinking to complete qualitative interviews. These women had previously completed a clinical alcohol intervention trial and had consented to be contacted in the future for study-related purposes. The biopsychosocial model was used to frame the interview questions that assessed multiple determinants of drinking behavior and helped explain linkages to broader health constructs. Results: A total of 20 women with HIV and hazardous drinking completed the qualitative interview. Several factors were identified by the women as influential in their decision to participate in the WHAT-IF? study, such as the ability to quit or reduce their drinking to nonhazardous levels (biological), the ability to gain knowledge or a greater understanding of the negative effects of hazardous drinking on HIV disease progression (psychological), and peer pressure and monetary compensation (social). Also, the women identified factors (positive or negative) associated with their clinical trial experience, such as the effects of the study medication on the woman's body (biological), thoughts and feelings toward study procedures (i.e. medication, lab work, study assessments) and the length of the study (psychological), and the interactions with the WHAT-IF? Study staff (social). Conclusion: Recruiting and retaining women with or without HIV in clinical research remains a challenge. Findings from this study suggest that women with HIV who are hazardous drinkers may benefit from participating in research studies that could help them to reduce or quit their drinking, increase their knowledge about specific behavior changes, and earn monetary compensation. Also, positive staff interactions may be instrumental in retaining minority women in clinical research.
Does Health Literacy Affect the Uptake of Annual Physical Check-Up?: The Varying Impact by Age Groups
Authors: Lee Hee Yun , Kim Sooyoung , Neese Jessica , Lee Mi Hwa 
Published: February 2020

Abstract
Introduction Little is known about how health literacy is linked to physical check-ups. This study aimed to examine the levels of physical check-ups by age group and the role of health literacy regarding physical check-ups. Methods Data for the study were obtained from the 2017 Health Information National Trends Survey. The original sample included 3,285 respondents, but only 3,146 surveys were used for this study. Andersen’s Behavioral Model of Health Services Use (Andersen, 1995) guided this study, and a binomial logistic regression model was conducted using Stata 12.0 software package. Results Annual check-ups were reported by 82.0% of the older group and 67.3% of the younger group. Both groups had similar ratios for health literacy-related item reporting. Study results show that annual check-up was positively associated with confidence in getting health information, having health insurance, and having a primary doctor for both age groups. However, getting a regular check-up was negatively associated with frustration while searching for information among the younger group while it was positively associated with difficulty understanding information for the older group. Conclusions To increase annual physical check-ups, health literacy-related interventions should be developed and address the barriers most associated with health check-ups. One way of addressing this barrier is to improve the line of communication from healthcare professionals to consumers through the use of easy-to-understand explanations appropriate for the consumer.
Kinetics and Specificity of HEK293T Extracellular Vesicle Uptake using Imaging Flow Cytometry
Authors: Jurgielewicz Brian , Yao Yao , Stice Steven L. 
Published: February 2020

Abstract
Background : Extracellular vesicles (EVs) are nanosized vesicles naturally secreted from cells responsible for intercellular communication and delivery of proteins, lipids, and other genetic material. Ultimately, EVs could provide innate therapeutic contents and loaded therapeutic payloads such as small molecules and gene therapy vectors to recipient cells. However, comparative kinetic measures that can be used to quantify and ultimately optimize delivery and uptake of EV payloads are lacking. We investigated both dose and time effects on EV uptake and evaluated the potential specificity of EV uptake to better understand the kinetics and uptake of human embryonic kidney (HEK293T) derived EVs. Results : Utilizing an imaging flow cytometry platform (IFC), HEK293T EV uptake was analyzed. HEK293T EV uptake was dose and time dependent with a minimum threshold dose of 6,000 EVs per cell at 4 hours of co-culture. HEK293T EV uptake was inhibited when co-cultured with recipient cells at 4°C or with pre-fixed recipient cells. By co-culturing HEK293T EVs with cell lines from various germ layers, HEK293T EVs were taken up at higher quantities by HEK293T cells. Lastly, human neural stem cells (hNSCs) internalized significantly more HEK293T EVs relative to mature neurons. Conclusions : Imaging flow cytometry is a quantitative, high throughput, and versatile platform to quantify the kinetics of EV uptake. Utilizing this platform, dose and time variables have been implicated to affect EV uptake measurements making standardization of in vitro and in vivo assays vital for the translation of EVs into the clinic. In this study, we quantified the selectivity of EV uptake between a variety of cell types in vitro and found that EVs were internalized at higher quantities by cells of the same origin. The characterization of HEK293T EV uptake in vitro, notably specificity, dose response, and kinetic assays should be used to help inform and develop EV based therapeutics.
Kinect Based System and Serious Game Motivating Approach for Physiotherapy Assessment and Remote Session Monitoring
Authors: Cary Francisco , Postolache Octavian , Gira˜o Pedro Silva 
Published: February 2020

Abstract
This paper describes a framework focused on being a health monitoring platform as well as a source of entertainment for physiotherapy patients, particularly stroke survivors. Using the Microsoft Kinect sensor a serious game captures 3D data during the patient’s session and sends it to a server. Through a web application these data can be further visualized by the physiotherapists on their assessments. Moreover, this framework intends to be a useful and effective tool for remote physiotherapy sessions allowing for a considerable cost reduction. Experimental results related to arms and shoulders rehabilitation are included in this paper.
Sleep in relation to psychiatric symptoms and perceived stress in Swedish adolescents aged 15 to 19 years
Authors: Thorsén Frida , Antonson Carl , Sundquist Jan , Sundquist Kristina 
Published: February 2020

Abstract

Background:
Sleep affects psychiatric health and perceived stress during adolescence.

Objective:
The first aim of this study was to investigate the prevalence of poor sleep in a sample of Swedish adolescents aged 15 to 19 years. The second aim was to investigate correlations between: a) sleep and psychiatric symptoms and; b) sleep and perceived stress. The third aim was to examine possible sex differences in sleep.

Method:
In 2011, a total of 185 Swedish adolescents (aged 15 to 19 years) from two upper secondary schools participated in this cross-sectional study. We used three different psychometric scales: Pittsburgh Sleep Quality Index (PSQI), Symptoms Checklist (SCL-90), and Perceived Stress Scale (PSS) to measure sleep, general psychiatric health and perceived stress.

Results:
In total, 76% of the female students and 71% of the male students had poor overall sleep quality. A large majority, 93%, reported daytime dysfunction and 60% reported problems staying awake during daily activities. The correlation between sleep and general psychiatric health was 0.44 and the correlation between sleep quality and perceived stress was 0.48. Female students reported significantly more sleep disturbances than male students do.

Conclusions:
Three out of four of the upper secondary school students presented with poor overall sleep that associated with psychiatric symptoms and perceived stress. These findings add to results from earlier studies and imply that interventions to improve sleep in adolescents, individually as well as on a societal level, should be considered as one way of trying to impact the observed rising numbers of psychiatric complaints. Such interventions may improve mental and somatic health in adolescents and prevent the development of psychiatric and stress-related symptoms. Further studies of possible methods, and their implementation, for improving sleep in adolescents should be of high priority.
Adolescentes en Internet: la mediación entre riesgos y oportunidades
Authors: Osorio Tamayo Dora Liliana , Millán Otero Katy Luz 
Published: February 2020

Se presenta una investigación realizada con el objetivo de describir los significados asociados a situaciones problemáticas y riesgos, oportunidades y mediaciones en el uso de Internet, identificadas por adolescentes, padres/madres y profesorado de instituciones educativas de la ciudad de Medellín, Colombia. El método se basó en un diseño cualitativo, concertado con el equipo de Kids online Latinoamérica (Latamko). Los resultados resaltan las tensiones entre los significados de oportunidad y riesgo en el uso de la Internet, de manera que se interrogó la mediación de padres y docentes, haciendo visibles límites en el aprovechamiento de las TIC atribuidos a la heterogeneidad y la brecha digital entre adultos y jóvenes. Como conclusión, se comenta la percepción de insuficiencia que tienen los jóvenes frente a la actuación de padres/madres y docentes, quienes se debaten entre prevenir e incentivar la apropiación de las herramientas para el aprendizaje.
Dynamic performance analysis for wind turbine in complex conditions
Authors: Cao Li , Sun Wenlei , Zhou Jianxing , Cui Quanwei 
Published: February 2020

The effect of dynamic performance shall be considered when calculating the wind speed relative to the wind turbine structure, since it is essential to prolong its service life. This article presents a method to get dynamic responses of a wind turbine under different conditions. The time-varying load acting on the blade is calculated by using the blade element momentum theory, and the dynamic performance of the wind turbine are calculated by applying the modal superposition method with blade loads as excitations. A platform is constructed to experimentally test the dynamic responses of the wind turbine system. The dynamic response process is adopted to carry out a dynamic analysis, and theoretical results are compared with experimental results, indicated that the analysis presented in this paper is correct. In addition, the 2 MW wind turbine operating in different wind fields is analyzed by applying the computing method. The results indicate that the wind turbine experiences a huge transverse vibration under turbulent wind, the hub vibration is intensified up to 179.52 %, and the vibration of the blade tip intensifies up to 190.41 % under the action of gusts in extreme conditions relative to the steady state, which shall be considered during design.
Seismic performance analysis of steel beam to CFST column connection with ductility and energy dissipation components
Authors: Wang Jingxuan , Li Qiuying , Wei Guoqiang 
Published: February 2020

Concrete-filled steel tubular (CFST) column to steel beam joint with the ductility and energy dissipation components is a type of connection which is used in prefabricated structures, to improve the capacity of connections and construction efficiency. In this paper, two different type of steel beam to CFST column connections with the penetrated high-strength bolts and end-plate are investigated, i.e., steel beam to CFST column connection with end plate (CJ-1), and T-stub bolted (CJ-2) connections. The finite element model (FEM) of steel beam to CFST column connection with the penetrated high-strength bolts under cyclic loading are conducted based on the whole process of the nonlinear explicit analysis method using ABAQUS. The feasibility of FEM is verified by a set of experimental results performed by our research group, as well as available test results from other researchers. The failure modes, bearing capacity, energy dissipation capacity and ductility and rigidity degeneration were studied. As a result, the load-displacement hysteretic loop curve of CJ-2 connection is plump. However, the hysteresis curve of CJ-1 shows pinching phenomenon. The value of buckling load and ultimate load of CJ-2 increased by 28 % and 30 % respectively, compared with CJ-1. With respect of stress analysis, the plastic strain accumulation position distribution is relatively uniform duo to the T-stub connection, avoiding the penetrated high-strength bolt early yield or fracture. The results show that the steel beam to CFST column connection with penetrated bolts and T-stub connection has good seismic capacity.