bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Epidemiological Characteristics of Deaths with COVID-19 in The Early Stage in Wuhan, China
Authors: Bai Jianjun , Shi Fang , Cao Jinhong , Wen Haoyu , Wang Fang , Mubarik Sumaira , Liu Xiaoxue , Yu Yong , Ding Jianbo , Yu Chuanhua 
Published: October 2020

Abstract
Objectives To analyze the epidemiological characteristics of deaths of COVID-19 in Wuhan, China and understand the changing trends of the COVID-19 epidemic and the effects of prevention and control measures in Wuhan.Methods Through the China's Infectious Disease Information System, we collected deaths’ information in Wuhan. We analyzed the patient's demographic characteristics, drew epidemiological curve, made distribution map of epidemic situation, etc. @Risk for fitting distribution, SPSS for statistical analysis, and ArcGIS for mapping.Results As of February 24, 2020, a total of 1833 unique deaths were extracted. Among the deaths with COVID-19, the mild type accounted for the most, 37.2%, followed by severe type, 30.1%. The median age was 70.0 (inter quartile range: 63.0-79.0) years, most of the deaths were distributed in 50-89 age group; no deaths occurred in 0-9 age group; and the male to female ratio was 1.95. A total of 65.7% of the deaths in Wuhan combined with underlying diseases, and the deaths with underlying diseases were mainly male; the main combined underlying diseases were hypertension, diabetes and cardiovascular diseases. The peak of daily deaths appeared on February 14 and then declined after February 14. The median interval from symptom onset to diagnosis was 10.0 (6.0-14.0) days; the interval from onset to diagnosis gradually shortened. The median interval from diagnosis to death was 6.0 (2.0-11.0)days; The median interval from symptom onset to deaths was 17.0 (12.0-22.0)days, respectively. In terms of geographical distribution, the central urban area was more serious. Wuchang District had the highest number of deaths, and Jianghan District had the highest death rate.Conclusion COVID-19 posed a greater threat to the elderly and more men than women, especially elderly men with underlying diseases. The geographical distribution showed that the epidemic in the central area of Wuhan is more serious than in the surrounding areas. Analysis of deaths as of February 24 indicates that the COVID-19 epidemic in Wuhan has achieved a tremendous improvement, and the strong epidemic control measures taken by Wuhan Government were very effective.
DEPDC1/ EEF1A1 Complex Promotes the Progression of Human Osteosarcoma via Downregulation of FOXO3a
Authors: Shen Lin , Lin Han , Zhang Aijun , Liu Ronghan , Zhou Chendan , Zhang Ying , Zhao Kai , Lu Laitong , Ning Bin 
Published: October 2020

Abstract
BackgroundOsteosarcoma is a common primary malignant bone tumor with poor prognosis. Currently there is no effective therapeutic strategies primarily due to the insufficient understanding its underlying mechanisms. Here we aimed to decipher the molecular mechanisms underlying the osteosarcoma progression.MethodsGEO data analysis, immunohistochemistry, qRT-PCR and western blotting were performed to evaluate the expression of differentially genes in human osteosarcoma tissues. Stably transfected human osteosarcoma cells were injected in mouse model to assess the effect of DEPDC1 in vivo. The function of DEPDC1–EEF1A1–FOXO3a axis was detected by mass spectrometry analysis, co-immunoprecipitation (co-IP) experiments and RNA sequencing in vitro. ResultsBy exploring differentially expressed genes, we found DEPDC1 is highly expressed in human osteosarcoma cells and tissues. Mechanistically, we found the protein expressed by DEPDC1 can directly bind to EEF1A1 through three binding regions, thus forming a complex. Importantly, DEPDC1/EEF1A1 complex can directly inhibit the transcription and expression of FOXO3a in vitro and in vivo, thus promoting the metastasis and proliferation of osteosarcoma. The clinical relevance study showed that overexpression of DEPDC1/EEF1A1 complex is correlated with reduced survival rate of osteosarcoma patients.ConclusionsCollectively, this study demonstrated the DEPDC1/EEF1A1–FOXO3a axis as a critical pathway that promotes the progression of osteosarcoma and leads to poor prognosis. Genetically targeting or pharmacologically inhibiting DEPDC1/EEF1A1–FOXO3a axis may serve a promising strategy for targeting human osteosarcoma.
Duodenal Stump Reinforcement with Purse-string Suture in Distal Gastrectomy under Laparoscope Combined with Uncut Roux-en-Y Reconstruction in Patients with Gastric Cancer
Authors: Li Fengyuan , Xu Jianghao , Xu Hao , Wang Weizhi , Zhang Diancai , Yang Li , Wang Linjun , Ge Yugang , Xia Yiwen , Shi Liang , Wang Lu , Xu Zekuan 
Published: October 2020

Abstract
Background: This study aimed to compare patient outcomes after laparoscopic distal gastrectomy with uncut Roux-en-Y reconstruction for gastric cancer between a group who underwent a duodenal stump reinforcement procedure and those who did not.Methods: Data from 233 patients with gastric cancer (GC) undergoing distal gastrectomy under laparoscope combined with uncut Roux-en-Y reconstruction were retrospectively investigated. Patients were divided into two groups. The non-reinforcement group (NR) underwent surgery from June 2014 to March 2015 with no reinforcement of the duodenal stump (n=54) and the reinforcement group (R) underwent surgery from April 2015 to June 2018 with reinforcement of the duodenal stump (n=179). In group R, the duodenum was divided using an endoscope-assisted linear stapler, which was reinforced by a purse-string suture along the duodenal staple line. Results: Duodenal stump leakage was observed in 2 patients from group NR (3.7%), while no duodenal stump leakage or fistula was detected in group R. In addition, no significant difference was observed in the patient characteristics between group NR and R.Conclusions: The incidence of duodenal stump leakage can be reduced by reinforcement with a purse-string suture.
A novel human multiple myeloma cell line with genetic abnormality of 1q21 gains only and CKS1B overexpression
Authors: Yi Ke , Zhang Hui , Zhang Yajun , Fu Weijun , Hou Jian , Li Rong 
Published: October 2020

Abstract
Background

Multiple myeloma (MM) remains an incurable hematologic malignancy mainly due to its cytogenetic abnormalities. It is important to establish permanent malignant MM cell lines as effective tools to develop more effective therapies.
Results

We established and characterized a new multiple myeloma (MM) cell line CZ2 from the pleural effusion of a 70-year-old man. Using nephelometry and flow cytometry, cells with typical plasma cell morphology but not classical plasma cell phenotype were found to be non-immunoglobulin-secretary cells. FISH analysis of cells revealed a unique characteristic that there is only gain of 1q21 region(1q21+), while no other common cytogenetic abnormalities of multiple myeloma such as deletion of 17p(17p-), deletion of 13q(13q-) and translocation of IgH. In addition, the original cell line maintains its single cytogenetic abnormality. Meanwhile, CKS1B, an adverse prognostic gene which is located in 1q21 region was highly expressed in CZ2 using western blotting. Knockdown of CKS1B could reduce cell viability in addition that cleaved PARP and cleaved caspase3 would be decreased.
Conclusions

Therefore, CZ2 provides a suitable material for cellular and molecular studies of multiple myeloma with only 1q21 abnormality. The cell line characterized by gain of 1q21 and high expression of CKS1B is an important model for studies of myeloma cell growth and drug resistance during therapy.
Electronic Clinical Decision Support for Children with Minor Head Trauma and Intracranial Injuries: A Sociotechnical Analysis
Authors: Greenberg Jacob K , Otun Ayodamola , Nasraddin Azzah , Brownson Ross C , Kuppermann Nathan , Limbrick David D , Yen Po-Yin , Foraker Randi E 
Published: October 2020

Abstract
Background: Current management of children with minor head trauma (MHT) and intracranial injuries is not evidence-based and may place some children at risk of harm. Evidence-based electronic clinical decision support (CDS) for management of these children may improve patient safety and decrease resource use. To guide these efforts, we evaluated the sociotechnical environment impacting the implementation of electronic CDS, including workflow and communication, institutional culture, and hardware and software infrastructure, among other factors. Methods: Between March and May, 2020 semi-structured qualitative focus group interviews were conducted to identify sociotechnical influences on CDS implementation. Physicians from neurosurgery, emergency medicine, critical care, and pediatric general surgery were included, along with information technology specialists. Participants were recruited from nine health centers in the United States. Focus group transcripts were coded and analyzed using thematic analysis. The final themes were then cross-referenced with previously defined sociotechnical dimensions.Results: We included 28 physicians and four information technology specialists in seven focus groups (median five participants per group). Five physicians were trainees and 10 had administrative leadership positions. Through inductive thematic analysis, we identified five primary themes: 1) clinical impact; 2) stakeholders and users; 3) tool content; 4) clinical practice integration; and 5) post-implementation evaluation measures. Participants generally supported using CDS to determine an appropriate level-of-care for these children. However, some had mixed feelings regarding how the tool could best be used by different specialties (e.g. use by neurosurgeons versus non-neurosurgeons). Feedback from the interviews helped refine the tool content and also highlighted potential technical and workflow barriers to address prior to implementation. Conclusions: We identified key factors impacting the implementation of electronic CDS for children with MHT and intracranial injuries. These results have informed our implementation strategy and may also serve as a template for future efforts to implement health information technology in a multidisciplinary, emergency setting.
Mechanistic Insights Into the Role of the Chemokine CCL2/CCR2&nbsp;Axis in Dorsal Root Ganglia to Peripheral Inflammation and Pain Hypersensitivity
Authors: Dansereau Marc-André , Midavaine Élora , Bégin-Lavallée Valérie , Belkouch Mounir , Beaudet Nicolas , Longpré Jean-Michel , Mélik-Parsadaniantz Stéphane , Sarret Philippe 
Published: October 2020

Abstract
Background: Pain is reported as the leading cause of disability in the common forms of inflammatory arthritis conditions. Acting as a key player in nociceptive processing, neuroinflammation and neuron-glia communication, the chemokine CCL2/CCR2 axis holds great promise for controlling chronic painful arthritis. Here, we investigated how the CCL2/CCR2 system in the dorsal root ganglion (DRG) contributes to the peripheral inflammatory pain sensitization.Methods: Repeated intrathecal (i.t.) administration of the CCR2 antagonist, INCB3344 was tested for its ability to reverse the nociceptive-related behaviors in the tonic formalin and complete Freund’s adjuvant (CFA) inflammatory models. We further determined by qPCR the expression of CCL2/CCR2, SP and CGRP in DRG neurons from CFA-treated rats. Using DRG explants, acutely dissociated primary sensory neurons and calcium mobilization assay, we also assessed the release of CCL2 and sensitization of nociceptors. Finally, we examined by immunohistochemistry following nerve ligation the axonal transport of CCL2, SP and CGRP from the sciatic nerve of CFA-treated rats.Results: We first found that CFA-induced paw oedema provoked an increase in CCL2/CCR2 and SP expression in ipsilateral DRGs, which was decreased after INCB3344 treatment. This upregulation in pronociceptive neuromodulators was accompanied by an enhanced nociceptive neuron excitability on days 3 and 10 post-CFA, as revealed by the CCR2-dependent increase in intracellular calcium mobilization following CCL2 stimulation. In DRG explants, we further demonstrated that the release of CCL2 was increased following peripheral inflammation. Finally, the excitation of nociceptors following peripheral inflammation stimulated the anterograde transport of SP at their peripheral nerve terminals. Importantly, blockade of CCR2 reduced sensory neuron excitability by limiting the calcium mobilization and subsequently decreased peripheral transport of SP towards the periphery. Finally, pharmacological inhibition of CCR2 reversed the pronociceptive action of CCL2 in rats receiving formalin injection and significantly reduced the neurogenic inflammation as well as the stimuli-evoked and movement-evoked nociceptive behaviors in CFA-treated rats.Conclusions: Our results provide significant mechanistic insights into the role of CCL2/CCR2 within the DRG in the development of peripheral inflammation, nociceptor sensitization and pain hypersensitivity. We further unveil the therapeutic potential of targeting CCR2 for the treatment of painful inflammatory disorders.
A Retrospective Study of Pegylated Liposomal Doxorubicin Combined with S-1 and Low-dose Oxaliplatin as First-Line Treatment for Advanced Gastric Cancer
Authors: Ma Jian , Yang Quanliang , Bi Yanzhi , Xiao Min , Li Xiaoqian , Zhao Qiu , Ye Wei , Ji Wenjing , Ling Yang 
Published: October 2020

Abstract
Background: At present, the first-line treatment of advanced gastric cancer is oxaliplatin combined with fluorouracil (capecitabine or S-1). Is there a three-drug regimen in which the curative effect is higher than that of the two-drug regimen, but the side effects are not significantly increased? This retrospective study evaluated the efficacy and side effects of pegylated liposomal doxorubicin (PLD) combined with S-1 and low-dose oxaliplatin as first-line treatment for advanced gastric cancer.Methods: Advanced gastric cancer patients who received PLD combined with S-1 and low-dose oxaliplatin (D-SOX) or standard SOX as first-line chemotherapy from Jan 2015 to Dec 2018 were included in the study. Overall survival (OS), progression-free survival (PFS), response rate (RR), and safety were assessed. Results: A total of 52 patients were included in this study. There were 23 cases in D-SOX group and 29 cases in SOX group. The median PFS was 7.1 months (95% CI 5.66–8.54 months) in the SOX group and 9.1 months (95% CI 4.2–14.0 months) in the D-SOX group (P=0.036). The median OS was 12.5 months (95% CI 7.31–17.70 months) in the SOX group and 19.5 months (95% CI 16.23–22.7 months) in the D-SOX group (P=0.017). The incidence of treatment-related grade III-IV adverse events was no more than 10%. The incidence of neurotoxicity was significantly reduced in the D-SOX group.Conclusions: This study suggests that PLD combined with S-1 and low-dose oxaliplatin might be a safe and more effective treatment for advanced gastric cancer.
Identifying Factors Predicting Prolonged Rehabilitation After Simultaneous Bilateral Total Knee Arthroplasty: A Retrospective Observational Study
Authors: Takagawa Shu , Kobayashi Naomi , Yukizawa Yohei , Oishi Takayuki , Tsuji Masaki , Misumi Toshihiro , Inaba Yutaka 
Published: October 2020

Abstract
Background: Rehabilitation is an effective procedure for promoting functional recovery after simultaneous bilateral total knee arthroplasty (TKA); however, it has been cited as the single largest economic burden of medical care spending. Risk factors affecting prolonged rehabilitation utilization after simultaneous bilateral TKA have not been identified. We hypothesized that preoperative factors, including age, sex, body mass index, living alone, the knee society function score (KSS), the American society of anesthesiologists (ASA) grade, hemoglobin (Hb), albumin level, mean range of motion, and the Kellgren–Lawrence grade, would predict prolonged rehabilitation utilization.Methods: A total of 191 patients undergoing simultaneous bilateral TKA in a single hospital were enrolled. The successful compliance group included patients who completed their rehabilitation program and were able to return to their residence within 3 weeks after surgery (n=132), whereas the delayed group included the remaining patients (n=59). Logistic regression analysis was performed using preoperative factors. A prediction scoring system was created using the regression coefficients from the logistic regression model.Results: Logistic regression analysis revealed that age (β=-0.0870; P<0.01) and Hb (β=0.34; P<0.05) were significantly associated with prolonged rehabilitation programs, whereas body mass index (BMI), living alone, the KSS score, and the ASA class were not significantly associated with successful completion of rehabilitation programs; however, these factors contributed to the prediction scoring formula, which was defined as: score = 10 – (0.09×age) – (0.09×body mass index) – (0.56×living alone [alone: 1, others: 0]) + (0.03×KSS stairs) + (0.34×Hb) – (1.1×ASA class). The C-statistic for the scoring system was 0.748 (95% confidence interval, 0.672–0.824). The optimal cutoff point for balancing sensitivity and specificity was 3.5, with 66.6% sensitivity and 78.0% specificity.Conclusions: Older age and lower preoperative Hb were significantly associated with prolonged rehabilitation programs. We defined a new scoring formula using preoperative patient factors to predict prolonged rehabilitation utilization in patients undergoing simultaneous bilateral TKA.
Elevated ILC3s-related Inflammatory Factors May Promote Tendinopathy
Authors: Xu Peng , Yue Jiawei , Xu Xu , Wu Yumin 
Published: October 2020

Abstract
Background: The prevalence of tendinopathy has risen dramatically over the last few decades and has become a common and serious orthopedic problem in sports injury and elderly populations. Immune cells have been shown to be associated with tendinopathy, with the percentage of Group 3 Innate Lymphoid Cells (ILC3s) having been shown to be upregulated in tendon tissue compared with peripheral blood. Materials and Methods: We used flow cytometry to investigate the percentage of circulating ILC3s in patients with tendinopathy and controls patients; Realtime-PCR was performed to detect the mRNA levels of ILC3s surface markers, CD45, IL-23R, ICOS and ILC3s-related inflammatory factors and transcription factors IL-17A, IL-22 and RORC in tendon samples. Results: Our results showed that the proportion of ILC3s in the peripheral blood circulation of patients with tendinopathy has no significant difference from that of controls by flow cytometry for Lin‑IL-2R+IL-23R + cells; however, the surface marker of ILC3s was upregulated in tendon tissue. In addition, IL-23, a characteristic activating factor of ILC3s, was also upregulated. Relative mRNA expression levels of IL-17A and IL-22 were also upregulated in tendinopathy tendon tissue compared to control patients. Conclusions: These results suggest that ILC3s may be a potential immune factor for tendinopathy.
A Case of Pulmonary Artery Sarcoma
Authors: Liu Yukang , Hu Yan , Han Wei , Tang Jingqun , Liang Qingchun , Wu Fang , Yu Fenglei , Liu Wenliang 
Published: October 2020

Abstract
Background: pulmonary artery sarcoma is a rare mesenchymal tumor. The prognosis of this very infrequent disease remains poor,and surgical resection is important for obtaining the best outcome.Case Presentation: A 51-year-old male patient presented to our clinic due to the finding of the isolated lung mass in the left lower lobe of the lung. Preoperative pulmonary artery computed tomographic angiography scans showed a mass with the in the left pulmonary artery and an isolated mass in the left dorsal segment of the lung (RS6).Under extracorporeal circulation the left pneumonectomy was performed, the surgical resection margins were confirmed to be tumor-free on frozen section and all lymph nodes were free of tumor. Histopathology, immunohistochemistry, as well as fluorescence in situ hybridization(FISH) indicated the diagnosis of pleomorphic rhabdomyosarcomaConclusion: This case report describes a PAS which was successfully resected with pneumonectomy and concludes that biopsy may play a limited role in confirming the pathological type and pneumonectomy should be considered in the surgical treatment of PAS.
Adamantinoma-like Ewing Sarcoma in a Metatarsal Bone After Chemotherapy Treated with an Osteocutaneous Fibular Transfer: A Case Report.
Authors: Marais Yolandi Anne , Saini Aaron , Ferreira Nando , Reddy Kershinee , Zühlke Alexander , Rossouw Nelmarie , Zaharie Stefan Dan , Schubert Pawel Tomasz 
Published: October 2020

Abstract
Background: Adamantinoma-like Ewing sarcoma is a rare variant of Ewing sarcoma known to have cytokeratin expression and squamous differentiation. It occurs more commonly in the head and neck region with only a few cases reported in long bones of the limbs. It also harbours EWSR1-FLI1 gene fusion which is required for the diagnosis of Ewing sarcoma. We present a case of Ewing sarcoma that manifested Adamantinoma-like morphology only post chemotherapy. Chemotherapy has been reported to induce neuronal maturation and rhabdoid morphology in cases of Ewing sarcoma, but no reports of treatment induced squamous differentiation with P40/P63 expression have been demonstrated to date.Case presentation: An 11 year old boy presented with a one year history of an enlarging painless mass over the left first metatarsal. Initial biopsy showed Ewing sarcoma with typical morphological features and EWSR1 rearrangement confirmed on fluorescent in-situ hybridization. The patient underwent neo-adjuvant chemotherapy and a subsequent wide local excision with an ipsilateral pedicled osteocutaneous fibula transfer. Subsequent histological examination showed frank squamous differentiation in the soft tissue component with keratin pearl formation and P40/P63 expression which was not observed in the initial biopsy and is compatible with Adamantinoma-like Ewing sarcoma.Conclusion: This case describes Adamantinoma-like Ewing sarcoma with P40/P63 expression after neo-adjuvant chemotherapy treatment. This immunophenotype was not apparent on the initial biopsy. It remains uncertain as to the reason for this change. This variant carries a poorer clinical outcome compared to the more conventional variant and could pose a diagnostic challenge(s) particularly if it occurs in an older patient or as a metastatic lesion.