bluuebunny/crossref_metadata_2025_split huggingface dataset:
Abdominal FLASH irradiation reduces radiation-induced gastrointestinal toxicity for the treatment of ovarian cancer in mice
Authors: Levy Karen , Natarajan Suchitra , Wang Jinghui , Chow Stephanie , Eggold Joshua T. , Loo Phoebe E. , Manjappa Rakesh , Melemenidis Stavros , Lartey Frederick M. , Schüler Emil , Skinner Lawrie , Rafat Marjan , Ko Ryan , Kim Anna , H. Al-Rawi Duaa , von Eyben Rie , Dorigo Oliver , Casey Kerriann M. , Graves Edward E. , Bush Karl , Yu Amy S. , Koong Albert C. , Maxim Peter G. , Loo Billy W. , Rankin Erinn B. 
Published: December 2020

AbstractRadiation therapy is the most effective cytotoxic therapy for localized tumors. However, normal tissue toxicity limits the radiation dose and the curative potential of radiation therapy when treating larger target volumes. In particular, the highly radiosensitive intestine limits the use of radiation for patients with intra-abdominal tumors. In metastatic ovarian cancer, total abdominal irradiation (TAI) was used as an effective postsurgical adjuvant therapy in the management of abdominal metastases. However, TAI fell out of favor due to high toxicity of the intestine. Here we utilized an innovative preclinical irradiation platform to compare the safety and efficacy of TAI ultra-high dose rate FLASH irradiation to conventional dose rate (CONV) irradiation in mice. We demonstrate that single high dose TAI-FLASH produced less mortality from gastrointestinal syndrome, spared gut function and epithelial integrity, and spared cell death in crypt base columnar cells compared to TAI-CONV irradiation. Importantly, TAI-FLASH and TAI-CONV irradiation had similar efficacy in reducing tumor burden while improving intestinal function in a preclinical model of ovarian cancer metastasis. These findings suggest that FLASH irradiation may be an effective strategy to enhance the therapeutic index of abdominal radiotherapy, with potential application to metastatic ovarian cancer.
Interoceptive insular cortex participates in sensory processing of gastrointestinal malaise and associated behaviors
Authors: Aguilar-Rivera Marcelo , Kim Sanggyun , Coleman Todd P. , Maldonado Pedro E. , Torrealba Fernando 
Published: December 2020

AbstractThe insular cortex plays a central role in the perception and regulation of bodily needs and emotions. Its modular arrangement, corresponding with different sensory modalities, denotes a complex organization, and reveals it to be a hub that is able to coordinate autonomic and behavioral responses to many types of stimuli. Yet, little is known about the dynamics of its electrical activity at the neuronal level. We recorded single neurons in behaving rats from the posterior insula cortex (pIC), a subdivision considered as a primary interoceptive cortex, during gastrointestinal (GI) malaise, a state akin to the emotion of disgust in humans. We found that a large proportion of pIC neurons were modulated during the rodent compensatory behaviors of lying on belly (LOB) and Pica. Furthermore, we demonstrated that LOB was correlated with low-frequency oscillations in the field potentials and spikes at the theta (8 Hz) band, and that low-frequency electrical microstimulation of pIC elicited LOB and Pica. These findings demonstrate that pIC neurons play a critical role in GI malaise perception, and that the pIC influences the expression of behaviors that alleviate GI malaise. Our model provides an accessible approach at the single cell level to study innate emotional behaviors, currently elusive in humans.
DNA damage in lens epithelial cells exposed to occupationally-relevant X-ray doses and role in cataract formation
Authors: Udroiu Ion , Sgura Antonella , Chendi Agnese , Lasagni Lorenzo , Bertolini Marco , Fioroni Federica , Piccagli Vando , Moramarco Antonio , Romano Maria Grazia , Fontana Luigi , D’Alessio Daniela , Bruzzaniti Vicente , Rosi Antonella , Grande Sveva , Palma Alessandra , Giliberti Claudia , Iori Mauro , Piergallini Lorenzo , Sumini Marco , Isolan Lorenzo , Cucchi Giorgio , Compagnone Gaetano , Strigari Lidia 
Published: December 2020

AbstractThe current framework of radiological protection of occupational exposed medical workers reduced the eye-lens equivalent dose limit from 150 to 20 mSv per year requiring an accurate dosimetric evaluation and an increase understanding of radiation induced effects on Lens cells considering the typical scenario of occupational exposed medical operators. Indeed, it is widely accepted that genomic damage of Lens epithelial cells (LEC) is a key mechanism of cataractogenesis. However, the relationship between apoptosis and cataractogenesis is still controversial. In this study biological and physical data are combined to improve the understanding of radiation induced effects on LEC. To characterize the occupational exposure of medical workers during angiographic procedures an INNOVA 4100 (General Electric Healthcare) equipment was used (scenario A). Additional experiments were conducted using a research tube (scenario B). For both scenarios, the frequencies of binucleated cells, micronuclei, p21-positive cells were assessed with different doses and dose rates. A Monte-Carlo study was conducted using a model for the photon generation with the X-ray tubes and with the Petri dishes considering the two different scenarios (A and B) to reproduce the experimental conditions and validate the irradiation setups to the cells. The simulation results have been tallied using the Monte Carlo code MCNP6. The spectral characteristics of the different X-ray beams have been estimated. All irradiated samples showed frequencies of micronuclei and p21-positive cells higher than the unirradiated controls. Differences in frequencies increased with the delivered dose measured with Gafchromic films XR-RV3. The spectrum incident on eye lens and Petri, as estimated with MCNP6, was in good agreement in the scenario A (confirming the experimental setup), while the mean energy spectrum was higher in the scenario B. Nevertheless, the response of LEC seemed mainly related to the measured absorbed dose. No effects on viability were detected. Our results support the hypothesis that apoptosis is not responsible for cataract induced by low doses of X-ray (i.e. 25 mGy) while the induction of transient p21 may interfere with the disassembly of the nuclear envelop in differentiating LEC, leading to cataract formation. Further studies are needed to better clarify the relationship we suggested between DNA damage, transient p21 induction and the inability of LEC enucleation.
Increased immune marker variance in a population of invasive birds
Authors: Prüter Hanna , Franz Mathias , Twietmeyer Sönke , Böhm Niklas , Middendorff Gudrun , Portas Ruben , Melzheimer Jörg , Kolberg Holger , von Samson-Himmelstjerna Georg , Greenwood Alex D. , Lüschow Dörte , Mühldorfer Kristin , Czirják Gábor Árpád 
Published: December 2020

AbstractImmunity and parasites have been linked to the success of invasive species. Especially lower parasite burden in invasive populations has been suggested to enable a general downregulation of immune investment (Enemy Release and Evolution of Increased Competitive Ability Hypotheses). Simultaneously, keeping high immune competence towards potentially newly acquired parasites in the invasive range is essential to allow population growth. To investigate the variation of immune effectors of invasive species, we compared the mean and variance of multiple immune effectors in the context of parasite prevalence in an invasive and a native Egyptian goose (Alopochen aegyptiacus) population. Three of ten immune effectors measured showed higher variance in the invasive population. Mean levels were higher in the invasive population for three effectors but lower for eosinophil granulocytes. Parasite prevalence depended on the parasite taxa investigated. We suggest that variation of specific immune effectors, which may be important for invasion success, may lead to higher variance and enable invasive species to reduce the overall physiological cost of immunity while maintaining the ability to efficiently defend against novel parasites encountered.
Trends and patterns in the use of opioids among metastatic breast cancer patients
Authors: Shen Chan , Thornton J. Douglas , Newport Kristina , Schaefer Eric , Zhou Shouhao , Yee Nelson S. , Dodge Daleela , Leslie Douglas 
Published: December 2020

AbstractOpioid use among metastatic breast cancer (MBC) patients has not been well-studied. This study examined the trends and patterns of opioid use among working-age, privately insured patients diagnosed with MBC. Using MarketScan data, we identified female patients diagnosed with MBC in 2006–2015. We determined the proportion of patients who filled a prescription for an opioid and calculated days’ supply and daily morphine milligram equivalents (MMEs) from 1 year prior to diagnosis till 1 year after. We assessed the trend in opioid use over the 10-year study period and examined opioid usage patterns after the diagnosis of MBC. Among 24,752 patients included, 11,579 (46.8%) had an opioid prescription within 1 year before diagnosis of MBC, and 20,416 (81.4%) had an opioid prescription within 1 year after diagnosis. The proportion of patients with opioid prescriptions after diagnosis was relatively stable from 2006 to 2015. However, both the median daily MME and median days’ supply decreased over time with most of the decline from the subgroup of patients with prior prescription opioid use. Most patients received an opioid prescription in the first month after diagnosis (57.3%), dropping to approximately 20% from 3 to 12 months after diagnosis. Also, the median days’ supply increased substantially during the year after diagnosis for patients who received opioids (from 7 to 19). Most women with MBC require opioid analgesia within the first month after diagnosis. Judicious, long-term management of pain after diagnosis of MBC will continue to be necessary for many patients.
Etiology and outcomes of current posterior chamber phakic intraocular lens extraction
Authors: Hayakawa Hideki , Kamiya Kazutaka , Ando Wakako , Takahashi Masahide , Shoji Nobuyuki 
Published: December 2020

AbstractThis study was aimed to review the etiology and the outcomes of current posterior chamber phakic intraocular lens (Visian ICL, STAAR Surgical) extraction. This review comprised 770 eyes of 403 consecutive patients undergoing ICL extraction. We evaluated prevalence, etiology, uncorrected distance visual acuity (UDVA), corrected distance visual acuity (CDVA), predictability, and patient satisfaction. ICL extraction was required in 8 of 770 (1.0%) eyes. The most common reason was the progression of the pre-existing cataract formation in 5 eyes (63%), followed by residual refractive errors in 3 eyes (38%). Of the 7 eyes targeted for emmetropia, 7 (100%) and 6 (86%) achieved UDVAs of 20/40 and 20/20 or better, respectively. Three eyes (38%) showed no change in CDVA, 3 eyes (38%) gained 1 line, 2 eyes (25%) gained 3 or more lines. 88% and 100% were within ± 0.5 and 1.0 diopter (D), respectively, of the targeted correction. Patient satisfaction improved significantly, from 3.0 ± 1.4 preoperatively, to 8.0 ± 2.4 postoperatively. No vision-threatening complications occurred. ICL extraction was required in approximately 1% of ICL-implanted eyes. Visual and refractive outcomes were good, and patient satisfaction was overall high, even in ICL-extracted eyes.
Spatiotemporal dynamic evolution and driving factors of desertification in the Mu Us Sandy Land in 30 years
Authors: Han Xueying , Jia Guangpu , Yang Guang , Wang Ning , Liu Feng , Chen Haoyu , Guo Xinyu , Yang Wenbin , Liu Jing 
Published: December 2020

AbstractThe Mu Us Sandy Land is located in the middle of the farming pastoral ecotone of northern China. The direction of the development of desertification has a direct impact on the economy and development of the northern region. Six remote sensing images acquired during 1990–2017 served as data sources. Using an ENVI 5.3 and ArcGIS 10.3 platform an analysis was conducted of the dynamic changes nearly 30 years in desertified land using a center of gravity moving model, annual change rate, a transfer matrix, and an aeolian desertification index; the factors driving desertification were discussed. The research shows that the time period can be divided into three stages of desertification: development (1990–2000), rapid reversal (2000–2010), and stable reversal (2010–2017). A total of 1680 km2 of desertification were managed over the three stages. Spatially, the distribution of the center of desertification from west to east includes mild, moderate, severe, and extreme desertification, which is consistent with the spatial distribution trends of desertified land in the Mu Us Sandy Land. By the end of 2017, the degree of desertification of the Mu Us Sandy Land was in the central area > northwest > southwest > east > south. Nearly 30 years, the wind speed has decreased year by year at the rate of 0.1 m s−1, which directly reduce the ability to winds to transport soil in the Mu Us Sandy Land and promoted the reversal of desertification. From 1990 to 2010, the climate tended to become warmer and drier. Environmental protection policies along with human intervention and control of desertification have played important roles in reversing desertification. From 2010 to 2020, under the general background of a warm-wet climatic tendency, rational use of sand resources and strengthening scientific control of desertification inducing factors are the keys to reversing desertification.
Generation of KS-58 as the first K-Ras(G12D)-inhibitory peptide presenting anti-cancer activity in vivo
Authors: Sakamoto Kotaro , Masutani Teruaki , Hirokawa Takatsugu 
Published: December 2020

AbstractRas mutations (e.g., occur in K-Ras, N-Ras, and H-Ras) are one of the most desirable and promising drug targets in chemotherapy treatments for cancer. However, there are still no approved drugs directly targeting mutated Ras. In 2017, an artificial cyclic peptide, KRpep-2d, was discovered as the first selective inhibitor of K-Ras(G12D), the most frequent K-Ras mutation. Here, we report the generation of KS-58, a KRpep-2d derivative that is identified as a bicyclic peptide and possess unnatural amino acid structures. Our in vitro data and molecular dynamics simulations suggest that KS-58 enters cells and blocks intracellular Ras–effector protein interactions. KS-58 selectively binds to K-Ras(G12D) and suppresses the in vitro proliferation of the human lung cancer cell line A427 and the human pancreatic cancer cell line PANC-1, both of which express K-Ras(G12D). Moreover, KS-58 exhibits anti-cancer activity when given as an intravenous injection to mice with subcutaneous or orthotropic PANC-1 cell xenografts. The anti-cancer activity is further improved by combination with gemcitabine. To the best of our knowledge, this is the first report of K-Ras(G12D)-selective inhibitory peptide presenting in vivo anti-cancer activity. KS-58 is an attractive lead molecule for the development of novel cancer drugs that target K-Ras(G12D).
Aerosol droplet-size distribution and airborne nicotine portioning in particle and gas phases emitted by electronic cigarettes
Authors: Lalo Hélène , Leclerc Lara , Sorin Jérémy , Pourchez Jérémie 
Published: December 2020

AbstractThe reliable characterization of particle size distribution and nicotine delivery emitted by electronic cigarettes (ECs) is a critical issue in their design. Indeed, a better understanding of how nicotine is delivered as an aerosol with an appropriate aerodynamic size is a necessary step toward obtaining a well-designed nicotine transfer from the respiratory tract to the bloodstream to better satisfy craving and improve smoking cessation rates. To study these two factors, recent models of EC devices and a dedicated vaping machine were used to generate aerosols under various experimental conditions, including varying the EC power level using two different types of atomizers. The aerodynamic particle sizing of the resulting aerosol was performed using a cascade impactor. The nicotine concentration in the refill liquid and the aerosol droplet was quantified by liquid chromatography coupled with a photodiode array. The vaporization process and the physical and chemical properties of the EC aerosol were very similar at 15 watts (W) and 25 W using the low-power atomizer but quite distinct at 50 W using the high-power atomizer, as follows: (1) the mass median aerodynamic diameters ranged from 1.06 to 1.19 µm (µm) for low power and from 2.33 to 2.46 µm for high power; (2) the nicotine concentrations of aerosol droplets were approximately 11 mg per milliliter (mg/mL) for low power and 17 mg/mL for high power; and (3) the aerosol droplet particle phase of the total nicotine mass emitted by EC was 60% for low power and 95% for high power. The results indicate that varying the correlated factors (1) the power level and (2) the design of atomizer (including the type of coil and the value of resistance used) affects the particle-size distribution and the airborne nicotine portioning between the particle phase and the gas phase in equilibrium with the airborne droplets.
Accuracy of zero-heat-flux thermometry and bladder temperature measurement in critically ill patients
Authors: Bräuer Anselm , Fazliu Albulena , Perl Thorsten , Heise Daniel , Meissner Konrad , Brandes Ivo Florian 
Published: December 2020

AbstractCore temperature (TCore) monitoring is essential in intensive care medicine. Bladder temperature is the standard of care in many institutions, but not possible in all patients. We therefore compared core temperature measured with a zero-heat flux thermometer (TZHF) and with a bladder catheter (TBladder) against blood temperature (TBlood) as a gold standard in 50 critically ill patients in a prospective, observational study. Every 30 min TBlood, TBladder and TZHF were documented simultaneously. Bland–Altman statistics were used for interpretation. 7018 pairs of measurements for the comparison of TBlood with TZHF and 7265 pairs of measurements for the comparison of TBlood with TBladder could be used. TBladder represented TBlood more accurate than TZHF. In the Bland Altman analyses the bias was smaller (0.05 °C vs. − 0.12 °C) and limits of agreement were narrower (0.64 °C to − 0.54 °C vs. 0.51 °C to – 0.76 °C), but not in clinically meaningful amounts. In conclusion the results for zero-heat-flux and bladder temperatures were virtually identical within about a tenth of a degree, although TZHF tended to underestimate TBlood. Therefore, either is suitable for clinical use.German Clinical Trials Register, DRKS00015482, Registered on 20th September 2018, http://apps.who.int/trialsearch/Trial2.aspx?TrialID=DRKS00015482.
Effect of internet use and electronic game-play on academic performance of Australian children
Authors: Islam Md Irteja , Biswas Raaj Kishore , Khanam Rasheda 
Published: December 2020

AbstractThis study examined the association of internet use, and electronic game-play with academic performance respectively on weekdays and weekends in Australian children. It also assessed whether addiction tendency to internet and game-play is associated with academic performance. Overall, 1704 children of 11–17-year-olds from young minds matter (YMM), a cross-sectional nationwide survey, were analysed. The generalized linear regression models adjusted for survey weights were applied to investigate the association between internet use, and electronic-gaming with academic performance (measured by NAPLAN–National standard score). About 70% of the sample spent > 2 h/day using the internet and nearly 30% played electronic-games for > 2 h/day. Internet users during weekdays (> 4 h/day) were less likely to get higher scores in reading and numeracy, and internet use on weekends (> 2–4 h/day) was positively associated with academic performance. In contrast, 16% of electronic gamers were more likely to get better reading scores on weekdays compared to those who did not. Addiction tendency to internet and electronic-gaming is found to be adversely associated with academic achievement. Further, results indicated the need for parental monitoring and/or self-regulation to limit the timing and duration of internet use/electronic-gaming to overcome the detrimental effects of internet use and electronic game-play on academic achievement.
Electronic and geometric structure effects on one-electron oxidation of first-row transition metals in the same ligand framework
Authors: Boniolo Manuel , Chernev Petko , Cheah Mun Hon , Heizmann Philipp A. , Huang Ping , Shylin Sergii I. , Salhi Nessima , Hossain Md Kamal , Gupta Arvind K. , Messinger Johannes , Thapper Anders , Lundberg Marcus 
Published: December 2020

The MII to MIII oxidation of base metals in a pentapyridyl ligand framework with an exchangeable apical ligand is studied by electrochemistry, XRD and XAS, and interpreted based on DFT calculations.
Plecstatin-1 induces an immunogenic cell death signature in colorectal tumour spheroids
Authors: Wernitznig Debora , Meier-Menches Samuel M , Cseh Klaudia , Theiner Sarah , Wenisch Dominik , Schweikert Andreas , Jakupec Michael A , Koellensperger Gunda , Wernitznig Andreas , Sommergruber Wolfgang , Keppler Bernhard K 
Published: December 2020

Abstract
Organometallic metal(arene) anticancer agents were believed to confer low selectivity for potential cellular targets. However, the ruthenium(arene) pyridinecarbothioamide (plecstatin-1) showed target selectivity for plectin, a scaffold protein and cytolinker. We employed a three-dimensional cancer spheroid model and showed that plecstatin-1 limited spheroid growth, induced changes in the morphology and in the architecture of tumour spheroids by disrupting the cytoskeletal organization. Additionally, we demonstrated that plecstatin-1 induced oxidative stress, followed by the induction of an immunogenic cell death signature through phosphorylation of eIF2α, exposure of calreticulin, HSP90 and HSP70 on the cell membrane and secretion of ATP followed by release of high mobility group box-1.
Enhancing the hydrogen evolution activity of MoS2 basal planes and edges using tunable carbon-based supports
Authors: Ruffman Charlie , Gordon Calum K. , Gilmour J. T. A. , Mackenzie Frank D. , Garden Anna L. 
Published: December 2020

ΔGHads on the basal plane and edges of MoS2 can be tuned using carbon based supports. The ΔGHads value on the edge relates directly to the degree of charge transfer between MoS2 and support.
Achieving pure yellow, high-efficiency (EQE > 20%) electroluminescence from ultrathin emitting layer (0.6–2.0 nm) OLEDs having a rare aggregation-free heteroleptic platinum complex
Authors: Kidanu Hagos Tesfay , Chen Chin-Ti 
Published: December 2020

A novel platinum complex FBNNND shows rare non-aggregation in solid state and hence the same emission color both in solution and in solid state, which is drastically different from that of FPtOPhND, a classical platinum complex.