bluuebunny/crossref_metadata_2025_split huggingface dataset:
Alkaloids of Peganum harmala: Anticancer Biomarkers with Promising Outcomes
Authors: Jalali Atefeh , Dabaghian Farid , Zarshenas Mohammad M. 
Published: November 2020

Background:Cancer is a serious and growing global health issue worldwide. In the cancerous cells, the normal cell cycle has been disrupted via a series of irreversible changes. Recently, the investigations on herbal medicine and clarifying the phytochemicals potential in treat cancer has been increased. The combination of phytochemicals with conventional cancer treatment approaches can improve outcomes via advancing cell death, restraining cell proliferation and invasion, sensitizing cancerous cells, and promoting the immune system. Therefore, phytochemicals can be introduced as relevant complementary medicaments in cancer therapy. Peganum harmala L. (Zygophyllaceae) as a valuable medicinal herb, possesses various alkaloid ingredient.Objective:Pointing to the importance of new avenues for cancer management and P. harmala convincing effect in this field, this review strived to collect a frame to epitome possible scopes to develop novel medicines in cancer treatment.Methods:Keywords "Peganum harmala" and cancer, or chemotherapy, or anti-neoplasm were searched through the "Scopus" database up to 29th of February 2020. Papers linking to agriculture, chemistry, environmental, and genetics sciences were omitted and, papers centered on cancer were selected.Result and Discussion:In the current study, 42 related papers to cancer treatment and 22 papers on alkaloid bioactive components are collected from 72 papers. The β-carboline alkaloids derived from P. harmala, especially harmine, demonstrate notable anticancer properties by targeting apoptosis, autophagy, abnormal cell proliferation, angiogenesis, metastasis, and cytotoxicity. Based on the collected information, P. harmala holds significant anticancer activity. Considering the mechanism of the various anticancer drugs and their acting similarity to P. harmala, the alkaloids derived from this herb, particularly harmine, can introduce as a novel anticancer medicine solely or in adjuvant cancer therapy.
How to Achieve Near Zero Fluoroscopy During Radiofrequency Ablation of Atrial Fibrillation: A Strategy Used at Two Centers
Authors: Haji Abdul Q. , Naji Mohammed B. , Jamal Shakeel , Kanjwal Khalil 
Published: November 2020

Abstract:
Radiofrequency ablation for atrial fibrillation is currently the most effective rhythm control
strategy. These procedures, although safe, pose a risk for potential exposure to radiation and
can be time consuming. Radiation exposure during ablation can increase the risk of serious complications
in both patients as well as physicians. The overall procedure time also increases with use of
fluoroscopy. Advances in mapping technology, such as electroanatomic mapping, use of contact
force technology, intracardiac echocardiography and use of versatile sheaths and catheters has vastly
enhanced our ability to both shorten the procedure duration and minimize or even eliminate radiation
exposure. Use of near zero fluoroscopy technique is increasingly gaining acceptance in electrophysiology
centers. At this point, there is no uniform technique and various centers use individual
techniques based on their expertise and availability of various tools. There is need for a uniform
technique that is workflow friendly and widely accepted. There is a learning curve associated with
this technique and efforts should be made to incorporate zero fluoroscopic technique for ablation as
an essential part of electrophysiology training programs. In this paper, we present the strategy being
practiced at two centers, that involves a series of steps, to either decrease or eliminate the use
of fluoroscopy during atrial fibrillation ablation.
Building a DNS Tunneling Dataset
Authors: Al-Drees Mohammed , Almasri Marwah M. , Al-Akhras Mousa , Alawairdhi Mohammed 
Published: November 2020

Background:
Domain Name System (DNS) is considered the phone book of the Internet.
Its main goal is to translate a domain name to an IP address that the computer can understand. However,
DNS can be vulnerable to various kinds of attacks, such as DNS poisoning attacks and DNS
tunneling attacks.

Objective:
The main objective of this paper was to allow researchers to identify DNS tunnel traffic using
machine-learning algorithms. Training machine-learning algorithms to detect DNS tunnel traffic
and determine which protocol was used will help the community to speed up the process of detecting
such attacks.

Method:
In this paper, we considered the DNS tunneling attack. In addition, we discussed how attackers
can exploit this protocol to infiltrate data breaches from the network. The attack starts by encoding
data inside the DNS queries to the outside of the network. The malicious DNS server will receive
a small chunk of data decoding the payload and put it together at the server. The main concern is
that the DNS is a fundamental service that is not usually blocked by a firewall and receives less attention
from systems administrators due to a vast amount of traffic.

Results:
This paper investigates how this type of attack happens using the DNS tunneling tool by setting
up an environment consisting of compromised DNS servers and compromised hosts with the Iodine
tool installed in both machines. The generated dataset contains the traffic of HTTP, HTTPS,
SSH, SFTP, and POP3 protocols over the DNS. No features were removed from the dataset so that researchers
could utilize all features in the dataset.

Conclusion:
DNS tunneling remains a critical attack that needs more attention to address. DNS tunneled
environment allows us to understand how such an attack happens. We built the appropriate dataset
by simulating various attack scenarios using different protocols. The created dataset contains
PCAP, JSON, and CSV files to allow researchers to use different methods to detect tunnel traffic.
Benefaction of Herbals in the Management of Urolithiasis
Authors: Yadav Ankit , Das Rina , Mehta Dinesh Kumar , Yatin  
Published: November 2020

:
Kidney stone formation or Urolithiasis is a common problem over many centuries with no guarantee of effective
treatment and, a high recurrence rate. Urolithiasis is precipitation of insoluble and less soluble salt such as Oxalate and
Phosphate in the urinary tract causing obstruction in the urethra resulting in renal colic and, hematuria.
Approximately, 10-12% of the population in industrialized countries are severely affected by Urinary stones. In, only a few
geographical areas is stone disease rare, e.g., in the coastal areas of Japan and Germany. It was believed that, 11% of people
in India are suffering from urinary stone problems, and approximately 50% of these cases may lead to severe renal damage.
Ultimately it causes severe health issues in terms of urinary tract obstruction, severe pain, and infection that adversely affects
the health of individuals.
Diuretics and narcotic analgesic like drugs which are used to prevent and cure urolithiasis are not effective in all cases and
are costly, give quite common recurrences, risk long term fertility and, other potential side effects are observed. So, humans
are forced return to Nature for safe remedies using herbal treatment. A great number of Indian medicinal plants have been
investigated in the treatment of urolithiasis, and they have been reported to be safe and effective.
In the present review, an effort has been made to highlight such herbal medicines which are potentially effective in the
management of urolithiasis.
Pelatihan Pembelajaran Biologi Berbasis ICT bagi Guru Muhammadiyah DKI Jakarta
Authors: Maesaroh Maesaroh , Gufron Amirullah  , Eka Kartikawati  , Mega Elvianasti  
Published: November 2020

Perkembangan teknologi informasi dan komunikasi atau Information and Communication of Technology (ICT) tidak dipungkiri telah memasuki berbagai lini kehidupan, salah satunya dalam bidang pendidikan. Sistem teknologi informasi dan komunikasi dapat digunakan hampir pada semua sektor manajemnen pendidikan. Meluasnya pemanfaatan teknologi informasi dan komunikasi berdampak pada karakter peserta didik. Hal ini menjadikan proses pembelajaran yang menggunakan model pembelajaran yang up to date atau berbasis ICT. Perbedaan generasi antara pendidik atau guru dan peserta didik dapat menghasilkan sebuah gap dalam komunikasi belajar atau penyampaian materi yang efektif dan efisien. Dalam hal ini akan lebih baik jika guru dapat mengimbangi atau menyamakan pengetahuan dan keterampilan dalam bidang ICT guna pencapaian tujuan pembelajaran dengan baik. Kegiatan pengabdian pada masyarakat ini menggunakan metode pelatihan yang bekerjasama dengan majlis Dikdasmen PWM Muhammadiyah DKI Jakarta. Diketahui bahwa 1) penggunaan perangkat pembelajaran berbasis ICT diantaranya yang berupa personal Computer, Proyektor, Compact Disc, Internet dan Software dalam pembelajaran rutin oleh guru Biologi peserta pelatihan sebanyak 47 %; 2) seluruh peserta pelatihan pembelajaran biologi berbasis ICT menyatakan bahwa kegiatan ini bermanfaat untuk mereka sebagai guru biologi dengan persentase jawaban sebanyak 100 %. Hal ini menunjukkan bahwa guru bersemangat dan memiliki kemauan untuk belajar meningkatkan kompetensi terkait dengan pembelajaran berbasis ICT.
Pendampingan dalam Implementasi Strategi Pemasaran Produk Olahan Susu Sapi
Authors: Garside Annisa Kesy , Amallynda Ikhlasul 
Published: November 2020

Usaha Bunda is a small home industry producing several cow’s milk processed products located at Maron village, Pujon Kidul, Malang. The challenge faced by this owner is the marketing aspect to define the right marketing strategy to expand the coverage and increase competitiveness. The goal of this community dedication is to help formulating and implementing the marketing strategy for the Usaha Bunda home industry. The approach is advocation in the form of assistance. Several strategy proposed was consignation with several gift shops and tourist attraction, redesign the product packaging, and creating an online shop. The outcome of assistance in marketing strategy implementation was the owner’s better understanding on the knowledge and skill of product packaging and online marketing. Additionally, the owner also confirmed that there has been an omzet increase of 1.5 to 2 times as the result of the new packaging. The online shop opened at Tokopedia marketplace also helps to expand the product promotion and market coverage without geographical and time limitation.
Epidemiology of hereditary diseases in health care: the evolution from theory to technologies and vice versa
Authors: Назаренко Л.П. , Назаренко М.С. 
Published: November 2020

В настоящее время наиболее сильное изменение в области эпидемиологии наследственных болезней заключается в переходе от теоретических обобщений популяционной генетики к активному использованию молекулярно-генетических технологий в отношении тестирования отдельных индивидов. Это позволяет персонализировать оказание медицинской помощи, а также генерировать новые знания о грузе наследственных болезней в популяциях с их последующей трансляцией в практику здравоохранения.
At the present time the most dramatic changes in the epidemiology of hereditary diseases is the transition from theoretical principles of population genetics in public health to the active use of molecular genetic technologies for individuals. Genetic discoveries are the basis of individualized medicine, as well as the source of new knowledge about the load of hereditary diseases in populations with their subsequent translation into healthcare system.
Characterization of the genetic heterogeneity of populations through the load of hereditary pathology
Authors: Ельчинова Г.И. , Кадышев В.В. , Зинченко Р.А. 
Published: November 2020

На основании архивных данных лаборатории генетической эпидемиологии ФГБНУ «МГНЦ» по 11 российским популяциям предложен метод количественной оценки генетической гетерогенности популяций по грузу аутосомно-доминантной и аутосомно-рецессивной наследственной патологии. Показано, что наименьшей гетерогенностью характеризуется Краснодарский край, наибольшей - население Татарстана.
On the basis of archival data from the laboratory of genetic epidemiology of the RCMG for 11 Russian populations, a method for quantifying the genetic heterogeneity of populations based on the load of autosomal dominant and autosomal recessive hereditary pathology is proposed. It is shown that the Krasnodar region is characterized by the least heterogeneity, and the population of Tatarstan is the largest.
Analysis of the prevalence of rare (orphan) diseases («List 24») and the dynamics of detection of children's patients in the subjects of the Russian Federation
Authors: Комаров И.А. , Красильникова Е.Ю. , Александрова О.Ю. , Зинченко Р.А. , Куцев С.И. 
Published: November 2020

Проведен анализ распространенности редких (орфанных) заболеваний («Перечень 24») и динамики выявляемости пациентов детского возраста в 85 регионах РФ в период 2013-2019 гг. Показан ежегодный прирост пациентов в Федеральном регистре (2013 г. - 4962 больных, 2014 г. - 6761, 2015 г. - 7038, 2016 г. - 7625, 2017 г. - 8245, 2018 г. - 8639, 2019 г. - 9088). Динамика доли детей с заболеваниями из «Перечня 24» среди всех больных колебалась в небольших пределах (в среднем 50%). Большинством заболеваний (16 из 24) болеют в основном дети, но они встречаются также у взрослых. Распространенность заболеваний значительно варьировала между субъектами РФ. Улучшение информированности, а также ведение Федерального регистра способствуют эффективной выявляемости и своевременному назначению терапии в детском возрасте. Однако низкие значения распространенности заболеваний, проявляющихся до 1-го года жизни, говорят о сложностях их диагностики и недостаточной выявляемости.
The analysis of the prevalence of rare (orphan) diseases («List of 24») and the dynamics of detection of children’s patients in 85 subjects of the Russian Federation in the period 2013-2019 showed an annual increase in patients in the Federal register (2013 - 4962 patients, 2014 - 6761, 2015 - 7038, 2016 - 7625, 2017 - 8245, 2018 - 8639, 2019 - 9088). The dynamics of the share of children with diseases from the “List of 24” among all patients fluctuated within small limits (on average 50%). Most of the diseases (16 out of 24) are mostly between children, but they also occur in adults. The prevalence of diseases varied significantly between the subjects of the Russian Federation. Improving awareness, as well as maintaining a Federal register promote effective detection and timely treatment in children. However, the low prevalence of diseases that occur before the 1st year of life indicates the complexity of their diagnosis and lack of detection.
Analysis of the mutational spectrum of the SLC26A4 gene and its contribution to the etiology of inherited hearing loss in the indigenous population of Southern Siberia
Authors: Данильченко В.Ю. , Зыцарь М.В. , Маслова Е.А. , Бады-Хоо М.С. , Морозов И.В. , Бондарь А.А. , Посух О.Л. 
Published: November 2020

Мутации в гене SLC26A4 являются частой причиной потери слуха во многих регионах мира. В работе приводятся результаты молекулярно-генетического анализа (с использованием секвенирования по Сэнгеру) последовательности гена SLC26A4, впервые проведенного в выборке пациентов с потерей слуха неустановленной этиологии (n=232) из Республик Тыва и Алтай. Установлены контрастные различия патогенетического вклада мутаций в гене SLC26A4 в этиологию нарушения слуха у коренных жителей этих географически близких регионов: 28,2% - для тувинцев и 4,3% - для алтайцев. Выявлены как уже известные, так и новые патогенные варианты, а также широкий спектр полиморфных вариантов гена SLC26A4.
Mutations in the SLC26A4 gene are a common cause of hearing loss in many regions of the world. This paper presents the results of molecular genetic analysis (by Sanger sequencing) of the SLC26A4 sequence, first performed in the sample of patients with hearing loss of unknown etiology (n=232) from the Tyva Republic and the Altai Republic. Contrast differences of the pathogenic contribution of SLC26A4 mutations to the etiology of hearing impairment were revealed in the indigenous peoples of these geographically close regions: 28.2% for Tuvinians and 4.3% for Altaians. Both known and novel pathogenic variants as well as a wide range of polymorphic variants were found in the SLC26A4 gene sequence.
Population Genomics of Russian populations
Authors: Степанов В.А. 
Published: November 2020

Популяционная геномика человека является мощным современным подходом в популяционной генетике, базирующемся на технологиях геномного секвенирования, биоинформатики и анализа больших данных. Геномный анализ генетической вариабельности в популяциях является фундаментальной основой генетики болезней и разработки путей их диагностики, терапии и профилактики. В работе представлены собственные данные о геномном анализе генетического разнообразия населения России. Показано, что генофонд современных народов России формировался на протяжении многих тысяч лет в ходе совокупного влияния миграций, изоляции расстоянием, эффектов основателя и естественного отбора. Сформировавшиеся в ходе микроэволюции геномные паттерны современных популяций в существенной мере определяют композицию генетических факторов как частых хронических, так и редких моногенных заболеваний.
Human population genomics is a powerful modern approach in population genetics based on technologies of genomic sequencing, bioinformatics, and big data analysis. Genomic analysis of genetic variability in populations is a fundamental basis for the genetics of diseases and the development of ways for their diagnosis, therapy and prevention. The work presents the own data on the genomic analysis of the genetic diversity of the Russian populations. It is shown that the gene pool of modern populations of Russia was formed over many thousands of years by the combined effects of migrations, isolation by distance, founder effects and natural selection. The genomic patterns of modern populations formed during microevolution substantially determine the composition of genetic factors of both frequent chronic and rare monogenic diseases.
Features of pathogenic CFTR gene variants in CF patients with negative neonatal screening
Authors: Петрова Н.В. , Каширская Н.Ю. , Кондратьева Е.И. , Шерман В.Д. , Воронкова А.Ю. , Васильева Т.А. , Зинченко Р.А. 
Published: November 2020

Особенностями спектра патогенных вариантов гена CFTR у пациентов с отрицательным результатом по неонатальному скринингу на муковисцидоз по сравнению с пациентами с положительным результатом являются более высокая доля генотипов, ассоциированных с остаточной функцией поджелудочной железы, более высокая частота варианта 3849+10kbC>T и более низкая доля гомозигот по варианту F508del.
Features of the spectrum of pathogenic CFTR gene variants in CF patients with negative neonatal screening compared to positive screening CF patients include a significantly higher frequency of genotypes associated with residual pancreatic function, a higher frequency of 3849+10kbC>T, and a lower frequency of F508del variant homozygotes.
Diagnosis of milder forms of phenylketonuria in the population of the Sverdlovsk region
Authors: Дерябина С.С. , Лагутина О.В. , Беляева Т.И. , Никитина Н.В. , Подолина В.К. , Николаева Е.Н. 
Published: November 2020

В работе представлены результаты расширенного генетического исследования 39 детей, наблюдавшихся в КДЦ «Охрана здоровья матери и ребенка» (г. Екатеринбург) с диагнозом «гиперфенилаланинемия». Анализ результатов обследования детей (у 13-ти пробандов наличие варианта R408W в гетерозиготном состоянии) показал, что поиск рекуррентных вариантов гена с использованием панели для ДНК-диагностики не дает ожидаемой верификации диагноза и требует дополнительного тестирования. Прямое секвенирование гена PAH позволило подтвердить диагноз «фенилкетонурия» (ФКУ) у 35 пробандов (89,8%) - у них обнаружено по 2 измененных аллеля. Еще у 4-х пациентов обнаружен только один патогенный вариант. Исследование ДНК родителей и сибсов указало на семейный вариант наследования всех выявленных мутаций. В результате проведенного исследования спектр патологии гена PAH, характерный для популяции Свердловской области, претерпел существенные изменения, что требует нового подхода к генодиагностике данной наследственной патологии в регионе.
The paper presents the results of an extended genetic study of 39 children observed in the MC “Health Care of Mother and Child” (Ekaterinburg) with a «hyperphenylalaninemia». An analysis of the previously known results of the study in these patients (the p. R408W variant in the heterozygous in 13 probands) showed that the search for recurrent gene variants using the panel does not give the expected proving of the diagnosis and requires additional testing. Applying the «gold standard» of diagnosis - direct sequencing of the PAH gene, the diagnosis of phenylketonuria (PKU) was confirmed in 35 probands (89.8%) - 2 altered alleles were found in them, one pathogenic variant was found in 4 others. A study of the DNA of parents and siblings indicated a families’ variants of inheritance for all identified mutations. As a result of the study, the pathology spectrum of the PAH gene, currently in use for the population of the Sverdlovsk region, has undergone significant changes, which require a new approach to the genetic diagnosis of this hereditary pathology in the region.
Molecular genetic characteristics of phenylketonuria in the population of Kazakhstan
Authors: Салимбаева Д.Н. 
Published: November 2020

Целью исследования было изучение спектра и выявление этнических особенностей мутаций гена РАН у пациентов с ФКУ в Казахстане. В статье представлены результаты молекулярно-генетического обследования 88 пациентов с ФКУ из неродственных семей, из них 36 пациентов казахской национальности, 44 русских, 5 уйгуров и 3 узбека. Наиболее частой мутацией в гене РАН у казахов была мутация p.243Q (с частотой 0,250), у русских, уйгур и узбеков - мутация p.R408W с частотой 0,545, 0,400 и 0,333 соответственно. Только у казахов были выявлены следующие мутации в гене РАН: IVS4+5G>T, IVS10-14C>G (0,028), p.V230I (0,028), p.A300S (0,014), p.W187X (0,014), p.R158Q (0,014), p.Y387H (0,014), p.I65N (0,014), p.R243L (0,014), p.Val399= (0,014), c.326e>G (0,014), p.P119S (0,014). Мутации IVS7-3C>A и p.E390G гена РАН были обнаружены только у русских. Для пациентов уйгурской национальности этнически специфичной оказалась мутация p.R413P, для узбеков - мутации p.R261X, G188D, p.R252Q, c.826-829 ins/del4?. Полученные результаты позволили описать спектр и этнические особенности мутаций гена РАН в Казахстане.
The aim of the study was to study the spectrum and identify ethnic characteristics of mutations of PAH gene in patients with PKU in Kazakhstan. The article presents the results of molecular genetic examination of 88 patients with PKU from unrelated families, including 36 patients of Kazakh nationality, 44 Russians, 5 Uighurs and 3 Uzbeks. The most frequent mutation in the PAH gene in Kazakhs was p.243Q mutation (with a frequency of 0.250), in Russians, Uighurs and Uzbeks p.R408W mutation with a frequency of 0.545, 0.400 and 0.333, respectively. Only in Kazakhs, following mutations were identified in PAH gene: IVS4 + 5G> T, IVS10-14C> G (0.028), p.V230I (0.028), p.A300S (0.014), p.W187X (0.014), p.R158Q (0.014), p.Y387H (0.014), p.I65N (0.014), p.R243L (0.014), p.Val399 = (0.014), c.326e> G (0.014), p.P119S (0.014). Mutations IVS7-3C> A and p.E390G in PAH gene were found only in Russians. For patients of Uyghur nationality, mutation p.R413P turned out to be ethnically specific; for Uzbeks, the mutations p.R261X, G188D, p.R252Q, c.826-829 ins/del4?. The results obtained allowed us to describe the spectrum and ethnic characteristics of mutations of PAH gene in Kazakhstan.