bluuebunny/crossref_metadata_2025_split huggingface dataset:
MALDI-TOF MS and genomic analysis can make the difference in the clarification of canine brucellosis outbreaks
Authors: da Silva David Attuy Vey , Brendebach Holger , Grützke Josephine , Dieckmann Ralf , Soares Rodrigo Martins , de Lima Julia Teresa Ribeiro , Keid Lara Borges , Hofreuter Dirk , Al Dahouk Sascha 
Published: November 2020

AbstractBrucellosis is one of the most common bacterial zoonoses worldwide affecting not only livestock and wildlife but also pets. Canine brucellosis is characterized by reproductive failure in dogs. Human Brucella canis infections are rarely reported but probably underestimated due to insufficient diagnostic surveillance. To improve diagnostics, we investigated dogs in a breeding kennel that showed clinical manifestations of brucellosis and revealed positive blood cultures. As an alternative to the time-consuming and hazardous classical identification procedures, a newly developed species-specific intact-cell matrix-assisted laser desorption/ionization–time of flight mass spectrometry analysis was applied, which allowed for rapid identification of B. canis and differentiation from closely related B. suis biovar 1. High-throughput sequencing and comparative genomics using single nucleotide polymorphism analysis clustered our isolates together with canine and human strains from various Central and South American countries in a distinct sub-lineage. Hence, molecular epidemiology clearly defined the outbreak cluster and demonstrated the endemic situation in South America. Our study illustrates that MALDI-TOF MS analysis using a validated in-house reference database facilitates rapid B. canis identification at species level. Additional whole genome sequencing provides more detailed outbreak information and leads to a deeper understanding of the epidemiology of canine brucellosis.
Designing profitable, resource use efficient and environmentally sound cereal based systems for the Western Indo-Gangetic plains
Authors: Jat Hanuman S. , Kumar Virender , Datta Ashim , Choudhary Madhu , Yadvinder-Singh  , Kakraliya Suresh K. , Poonia Tanuja , McDonald Andrew J. , Jat Mangi L. , Sharma Parbodh C. 
Published: November 2020

AbstractIn the western Indo-Gangetic plains, issues of deterioration in soil, water, and environment quality coupled with low profitability jeopardize the sustainability of the dominant rice–wheat (RW) system. To address these issues, crop diversification and conservation agriculture (CA)-based management hold considerable promise but the adoption of both approaches has been low, and additional evidence generation from a multi-criteria productivity and sustainability perspective is likely required to help drive the change. Compared to prevailing farmers’ practice (FP), results suggest that CA-based rice management increased profitability by 13% and energy use efficiency (EUE) by 21% while reducing irrigation by 19% and global warming potential (GWP) by 28%. By substituting CA-based maize for rice, similar mean profitability gains were realized (16%) but transformative improvements in irrigation (− 84%), EUE (+ 231%), and GWP (− 95%) were observed compared to FP. Inclusion of mungbean in the rotation (i.e. maize-wheat-mungbean) with CA-based management increased the system productivity, profitability, and EUE by 11, 25 and 103%, respectively while decreasing irrigation water use by 64% and GWP by 106% compared to FP. Despite considerable benefits from the CA-based maize-wheat system, adoption of maize is not widespread due to uneven market demand and assured price guarantees for rice.
Hybrid perfect metamaterial absorber for microwave spin rectification applications
Authors: Qian Jie , Gou Peng , Pan Hong , Zhu Liping , Gui Y. S. , Hu C.-M. , An Zhenghua 
Published: November 2020

AbstractMetamaterials provide compelling capabilities to manipulate electromagnetic waves beyond the natural materials and can dramatically enhance both their electric and magnetic fields. The enhanced magnetic fields, however, are far less utilized than the electric counterparts, despite their great potential in spintronics. In this work, we propose and experimentally demonstrate a hybrid perfect metamaterial absorbers which combine the artificial metal/insulator/metal (MIM) metamaterial with the natural ferromagnetic material permalloy (Py) and realize remarkably larger spin rectification effect. Magnetic hot spot of the MIM metamaterial improves considerably electromagnetic coupling with spins in the embedded Py stripes. With the whole hybridized structure being optimized based on coupled-mode theory, perfect absorption condition is approached and an approximately 190-fold enhancement of spin-rectifying photovoltage is experimentally demonstrated at the ferromagnetic resonance at 7.1 GHz. Our work provides an innovative solution to harvest microwave energy for spintronic applications, and opens the door to hybridized magnetism from artificial and natural magnetic materials for emergent applications such as efficient optospintronics, magnonic metamaterials and wireless energy transfer.
Light and ripening-regulated BBX protein-encoding genes in Solanum lycopersicum
Authors: Lira Bruno Silvestre , Oliveira Maria José , Shiose Lumi , Wu Raquel Tsu Ay , Rosado Daniele , Lupi Alessandra Cavalcanti Duarte , Freschi Luciano , Rossi Magdalena 
Published: November 2020

AbstractLight controls several aspects of plant development through a complex signalling cascade. Several B-box domain containing proteins (BBX) were identified as regulators of Arabidopsis thaliana seedling photomorphogenesis. However, the knowledge about the role of this protein family in other physiological processes and species remains scarce. To fill this gap, here BBX protein encoding genes in tomato genome were characterised. The robust phylogeny obtained revealed how the domain diversity in this protein family evolved in Viridiplantae and allowed the precise identification of 31 tomato SlBBX proteins. The mRNA profiling in different organs revealed that SlBBX genes are regulated by light and their transcripts accumulation is directly affected by the chloroplast maturation status in both vegetative and fruit tissues. As tomato fruits develops, three SlBBXs were found to be upregulated in the early stages, controlled by the proper chloroplast differentiation and by the PHYTOCHROME (PHY)-dependent light perception. Upon ripening, other three SlBBXs were transcriptionally induced by RIPENING INHIBITOR master transcriptional factor, as well as by PHY-mediated signalling and proper plastid biogenesis. Altogether, the results obtained revealed a conserved role of SlBBX gene family in the light signalling cascade and identified putative members affecting tomato fruit development and ripening.
Carotid sinus nerve stimulation attenuates alveolar bone loss and inflammation in experimental periodontitis
Authors: Ribeiro Aline Barbosa , Brognara Fernanda , da Silva Josiane Fernandes , Castania Jaci Airton , Fernandes Patrícia Garani , Tostes Rita C. , Salgado Helio Cesar 
Published: November 2020

AbstractBaroreceptor and chemoreceptor reflexes modulate inflammatory responses. However, whether these reflexes attenuate periodontal diseases has been poorly examined. Thus, the present study determined the effects of electrical activation of the carotid sinus nerve (CSN) in rats with periodontitis. We hypothesized that activation of the baro and chemoreflexes attenuates alveolar bone loss and the associated inflammatory processes. Electrodes were implanted around the CSN, and bilateral ligation of the first mandibular molar was performed to, respectively, stimulate the CNS and induce periodontitis. The CSN was stimulated daily for 10 min, during nine days, in unanesthetized animals. On the eighth day, a catheter was inserted into the left femoral artery and, in the next day, the arterial pressure was recorded. Effectiveness of the CNS electrical stimulation was confirmed by hypotensive responses, which was followed by the collection of a blood sample, gingival tissue, and jaw. Long-term (9 days) electrical stimulation of the CSN attenuated bone loss and the histological damage around the first molar. In addition, the CSN stimulation also reduced the gingival and plasma pro-inflammatory cytokines induced by periodontitis. Thus, CSN stimulation has a protective effect on the development of periodontal disease mitigating alveolar bone loss and inflammatory processes.
A local difference in blood–brain barrier permeability in the caudate putamen and thalamus of a rat brain induced by focused ultrasound
Authors: Huh Hyungkyu , Park Tae Young , Seo Hyeon , Han Mun , Jung Byeongjin , Choi Hyo Jin , Lee Eun-Hee , Pahk Ki Joo , Kim Hyungmin , Park Juyoung 
Published: November 2020

AbstractA blood–brain barrier (BBB) opening induced by focused ultrasound (FUS) has been widely studied as an effective way of treating brain diseases. We investigate the effect of ultrasound’s incidence angle at caudate putamen (Cp) and thalamus (Th) of the rat brain by inducing the same power of focused ultrasound that corresponds to the acoustic pressure of 0.65 MPa in free field. The BBB permeability (Ktrans) was quantitatively evaluated with dynamic contrast-enhanced magnetic resonance imaging. The group averaged (n = 11) maximum Ktrans at Cp (0.021 ± 0.012 min−1) was 1.39 times smaller than the Ktrans of Th (0.029 ± 0.01 min−1) with p = 0.00343. The group averaged (n = 6) ultrasound’s incidence angles measured using the computed tomography image of rat skulls were compared with the maximum Ktrans and showed a negatively linear relation R2 = 0.7972). The maximum acoustic pressure computed from the acoustic simulation showed higher average acoustic pressures at Th (0.37 ± 0.02 MPa) compared to pressures at Cp (0.32 ± 0.01 MPa) with p = 0.138 × 10−11. More red blood cell were observed at the Th region compared to the Cp region in the tissue staining. These results indicate that localized characteristics of the sonication target within the subject should be considered for safer and more efficient BBB disruption induced by FUS.
Implications of increasing Atlantic influence for Arctic microbial community structure
Authors: Carter-Gates Michael , Balestreri Cecilia , Thorpe Sally E. , Cottier Finlo , Baylay Alison , Bibby Thomas S. , Moore C. Mark , Schroeder Declan C. 
Published: November 2020

AbstractIncreasing influence of Atlantic water in the Arctic Ocean has the potential to significantly impact regional water temperature and salinity. Here we use a rDNA barcoding approach to reveal how microbial communities are partitioned into distinct assemblages across a gradient of Atlantic-Polar Water influence in the Norwegian Sea. Data suggest that temperate adapted bacteria may replace cold water taxa under a future scenario of increasing Atlantic influence, but the eukaryote response is more complex. Some abundant eukaryotic cold water taxa could persist, while less abundant eukaryotic taxa may be replaced by warmer adapted temperate species. Furthermore, within lineages, different taxa display evidence of increased relative abundance in reaction to favourable conditions and we observed that rare microbial taxa are sample site rather than region specific. Our findings have significant implications for the vulnerability of polar associated community assemblages, which may change, impacting the ecosystem services they provide, under predicted increases of Atlantic mixing and warming within the Arctic region.
Improving the abrasion resistance of Ti6Al4V alloy by modifying its surface with a diazonium salt and attaching of polyurethane
Authors: Sandomierski Mariusz , Buchwald Tomasz , Patalas Adam , Voelkel Adam 
Published: November 2020

AbstractCommonly used endoprostheses in the orthopedic industry are those made of Ti6Al4V titanium alloy. Unfortunately, this material has low abrasion resistance, and therefore methods of their modification are still sought. A sensible approach is coating the alloy with a layer of a polymer having higher abrasion resistance. The adhesion of polymers to alloy is low, therefore the alloy requires prior modification. In this work, the alloy was modified with three types of diazonium salt and the influence of substituent on the effectiveness of modification was determined. Then, five or ten polyurethane layers were attached to the surface of the modified alloy. Using Raman mapping, the uniform distribution of layers was proved. Layers are stable in simulated human body fluids. The effectiveness of attaching subsequent layers of polyurethane was also confirmed by nanoindentation. The main focus of this work was to improve the wear resistance of the titanium alloy. The obtained results indicate that the titanium alloy with a polyurethane layer has almost ten times lower coefficient of friction compared to pure alloy. Such a low value has not been described in the literature so far. These results are the first step for obtaining endoprostheses with very high abrasion resistance.
Identifying environmental risk factors for inflammatory bowel diseases: a Mendelian randomization study
Authors: Carreras-Torres Robert , Ibáñez-Sanz Gemma , Obón-Santacana Mireia , Duell Eric J. , Moreno Victor 
Published: November 2020

AbstractSeveral studies have examined environmental factors and inflammatory bowel diseases (IBD) using traditional approaches; however, provided results are still conflicting. Our aim was to determine whether lifestyle and nutrient exposures, related to IBD in observational meta-analyses, influence IBD risk using a Mendelian randomization (MR) approach. A two-sample MR approach was applied on summary-level genome-wide association results. Genetic variants strongly associated with measures of tobacco smoking, obesity and fat distribution, physical activity, and blood levels of vitamins and fatty acids were evaluated on genetic data from international IBD consortia including a total of 25,042 IBD cases (12,194 cases of Crohn’s disease (CD) and 12,366 cases of ulcerative colitis (UC)) and 34,915 controls. Our results indicated that, among lifestyle exposures, being a smoker was positively associated with CD (OR 1.13, P = 0.02), but it was not associated with UC risk (OR 0.99, P = 0.88). Body-mass index (BMI) and body fat percentage were positively associated with CD (OR 1.11, P = 0.02, per standard deviation (SD) of 4.6 kg/m2; and OR 1.50, P = 3 × 10–10, per SD of 6.6%; respectively); while for UC, BMI was inversely associated (OR 0.85, P = 5 × 10–5; per SD) and body fat percentage showed a OR of 1.11 (P = 0.11; per SD). Additionally, among nutrient exposures, omega-3 fatty acids levels were inversely associated with CD (OR 0.67, P = 2 × 10–6). Our MR results did not support a protective effect for being a smoker on UC risk; however, they are compatible with a risk effect for higher body fat proportion and a protective role for higher levels of omega-3 fatty acids on CD etiology.
Genetic evidence supports three previously described species of greater glider, Petauroides volans, P. minor, and P. armillatus
Authors: McGregor Denise C. , Padovan Amanda , Georges Arthur , Krockenberger Andrew , Yoon Hwan-Jin , Youngentob Kara N. 
Published: November 2020

AbstractThe identification and classification of species are essential for effective conservation management. This year, Australia experienced a bushfire season of unprecedented severity, resulting in widespread habitat loss and mortality. As a result, there has been an increased focus on understanding genetic diversity and structure across the range of individual species to protect resilience in the face of climate change. The greater glider (Petauroides volans) is a large, gliding eucalypt folivore. This nocturnal arboreal marsupial has a wide distribution across eastern Australia and is considered the sole extant member of the genusPetauroides. Differences in morphology have led to suggestions that the one accepted species is actually three. This would have substantial impacts on conservation management, particularly given a recent history of declining populations, coupled with extensive wildfires. Until now, genetic evidence to support multiple species has been lacking. For the first time, we used DArT sequencing on greater glider tissue samples from multiple regions and found evidence of three operational taxonomic units (OTUs) representing northern, central and southern groups. The three OTUs were also supported by our morphological data. These findings have important implications for greater glider management and highlight the role of genetics in helping to assess conservation status.
Thirty-day readmission rates, trends and its impact on liver transplantation recipients: a national analysis
Authors: Mumtaz Khalid , Lee-Allen Jannel , Porter Kyle , Kelly Sean , Hanje James , Conteh Lanla F. , Michaels Anthony J. , El-Hinnawi Ashraf , Washburn Ken , Black Sylvester M. , Abougergi Marwan S. 
Published: November 2020

AbstractReduction of early hospital readmissions is a declared goal in the United States economic and quality improvement agenda. A retrospective study was performed using the Nationwide Readmissions Database from 2010 to 2014. Our primary aim was to study the rate of early readmissions and its predictors in liver transplant recipients (LTRs). Our secondary aims were to determine the trends of LT, reasons for readmission, costs and predictors of calendar year mortality. Multivariable logistic regression and Cox proportional hazards models were utilized. The 30-day readmission rate was 30.6% among a total of 25,054 LTRs. Trends of LT were observed to be increased in patients > 65 years (11.7–17.8%, p < 0.001) and decreased in 40–64 years (78.0–73.5%, p = 0.001) during study period. The majority of 30-day readmissions were due to post transplant complications, with packed red blood cell transfusions being the most common intervention during readmission. Medicaid or Medicare insurance, surgery at low and medium volume centers, infections, hemodialysis, liver biopsy, and length of stay > 10 days were the predictors of 30-day readmission. Moreover, number of early readmission, age > 64 years, non-alcoholic cirrhosis, and length of stay > 10 days were significant predictor of calendar year mortality in LTRs. Approximately one third of patients require early admission after LT. Early readmission not only increases burden on healthcare, but is also associated with calendar year mortality. Strategies should be implemented to reduce readmission in patients with high risk of readmission identified in our study.
Comparative analysis of the complete mitochondrial genomes in two limpets from Lottiidae (Gastropoda: Patellogastropoda): rare irregular gene rearrangement within Gastropoda
Authors: Feng Jian-tong , Guo Ya-hong , Yan Cheng-rui , Ye Ying-ying , Li Ji-ji , Guo Bao-ying , Lü Zhen-ming 
Published: November 2020

AbstractTo improve the systematics and taxonomy of Patellogastropoda within the evolution of gastropods, we determined the complete mitochondrial genome sequences ofLottia goshimaiandNipponacmea fuscoviridisin the family Lottiidae, which presented sizes of 18,192 bp and 18,720 bp, respectively. In addition to 37 common genes among metazoa, we observed duplication of thetrnMgene inL. goshimaiand thetrnMandtrnWgenes inN. fuscoviridis. The highest A + T contents of the two species were found within protein-coding genes (59.95% and 54.55%), followed by rRNAs (56.50% and 52.44%) and tRNAs (56.42% and 52.41%).trnS1andtrnS2could not form the canonical cloverleaf secondary structure due to the lack of a dihydrouracil arm in both species. The gene arrangements in all Patellogastropoda compared with those of ancestral gastropods showed different levels of gene rearrangement, including the shuffling, translocation and inversion of single genes or gene fragments. This kind of irregular rearrangement is particularly obvious in the Lottiidae family. The results of phylogenetic and gene rearrangement analyses showed thatL. goshimaiandLottia digitalisclustered into one group, which in turn clustered withN. fuscoviridisin Patellogastropoda. This study demonstrates the significance of complete mitogenomes for phylogenetic analysis and enhances our understanding of the evolution of Patellogastropoda.
Submergence deactivates wound-induced plant defence against herbivores
Authors: Lee Hyo-Jun , Park Ji-Sun , Shin Seung Yong , Kim Sang-Gyu , Lee Gisuk , Kim Hyun-Soon , Jeon Jae Heung , Cho Hye Sun 
Published: November 2020

AbstractFlooding is a common and critical disaster in agriculture, because it causes defects in plant growth and even crop loss. An increase in herbivore populations is often observed after floods, which leads to additional damage to the plants. Although molecular mechanisms underlying the plant responses to flooding have been identified, how plant defence systems are affected by flooding remains poorly understood. Herein, we show that submergence deactivates wound-induced defence against herbivore attack inArabidopsis thaliana. Submergence rapidly suppressed the wound-induced expression of jasmonic acid (JA) biosynthesis genes, resulting in reduced JA accumulation. While plants exposed to hypoxia in argon gas exhibited similar reduced wound responses, the inhibitory effects were initiated after short-term submergence without signs for lack of oxygen. Instead, expression of ethylene-responsive genes was increased after short-term submergence. Blocking ethylene signalling byein2-1mutation partially restored suppressed expression of several wound-responsive genes by submergence. In addition, submergence rapidly removed active markers of histone modifications at a gene locus involved in JA biosynthesis. Our findings suggest that submergence inactivates defence systems of plants, which would explain the proliferation of herbivores after flooding.
Bacterial diets differentially alter lifespan and healthspan trajectories in C. elegans
Authors: Stuhr Nicole L. , Curran Sean P. 
Published: November 2020

AbstractDiet is one of the more variable aspects in life due to the variety of options that organisms are exposed to in their natural habitats. In the laboratory, C. elegans are raised on bacterial monocultures, traditionally the E. coli B strain OP50, and spontaneously occurring microbial contaminants are removed to limit experimental variability because diet—including the presence of contaminants—can exert a potent influence over animal physiology. In order to diversify the menu available to culture C. elegans in the lab, we have isolated and cultured three such microbes: Methylobacterium, Xanthomonas, and Sphingomonas. The nutritional composition of these bacterial foods is unique, and when fed to C. elegans, can differentially alter multiple life history traits including development, reproduction, and metabolism. In light of the influence each food source has on specific physiological attributes, we comprehensively assessed the impact of these bacteria on animal health and devised a blueprint for utilizing different food combinations over the lifespan, in order to promote longevity. The expansion of the bacterial food options to use in the laboratory will provide a critical tool to better understand the complexities of bacterial diets and subsequent changes in physiology and gene expression.