bluuebunny/crossref_metadata_2025_split huggingface dataset:
Overexpression of HbMBF1a, encoding multiprotein bridging factor 1 from the halophyte Hordeum brevisubulatum, confers salinity tolerance and ABA insensitivity to transgenic Arabidopsis thaliana
Authors: Zhang Lili , Wang Yunxiao , Zhang Qike , Jiang Ying , Zhang Haiwen , Li Ruifen 
Published: October 2019

Abstract
Key message
HbMBF1a was isolated and characterized in H. brevisubulatum, and overexpressed HbMBF1a could enhance the salt tolerance and ABA insensitivity in Arabidopsis thaliana. The transcript levels of stress-responsive genes were significantly increased in the transgenic lines under salt and ABA conditions.

Abstract
Salinity is an abiotic stress that considerably affects plant growth, yield, and distribution. Hordeum brevisubulatum is a halophyte that evolved to become highly tolerant to salinity. Multiprotein bridging factor 1 (MBF1) is a transcriptional coactivator and an important regulator of stress tolerance. In this study, we isolated and characterized HbMBF1a based on the transcriptome data of H. brevisubulatum grown under saline conditions. We overexpressed HbMBF1a in Arabidopsis thaliana and compared the phenotypes of the transgenic lines and the wild-type in response to stresses. The results indicated that HbMBF1a expression was induced by salt and ABA treatments during the middle and late stages. The overexpression of HbMBF1a in A. thaliana resulted in enhanced salt tolerance and ABA insensitivity. More specifically, the enhanced salt tolerance manifested as the increased seed germination and seedling growth and development. Similarly, under ABA treatments, the cotyledon greening rate and seedling root length were higher in the HbMBF1a-overexpressing lines, suggesting the transgenic plants were better adapted to high exogenous ABA levels. Furthermore, the transcript levels of stress-responsive genes were significantly increased in the transgenic lines under salt and ABA conditions. Thus, HbMBF1a is a positive regulator of salt and ABA responses, and the corresponding gene may be useful for producing transgenic plants that are salt tolerant and/or ABA insensitive, with few adverse effects. This study involved a comprehensive analysis of HbMBF1a. The results may provide the basis and insight for the application of MBF1 family genes for developing stress-tolerant crops.
The bicycle-train travellers in the Netherlands: personal profiles and travel choices
Authors: Jonkeren Olaf , Kager Roland , Harms Lucas , te Brömmelstroet Marco 
Published: October 2019

AbstractThe Netherlands seems to exhibit the unique conditions that allow cycling on the country level instead of only the city level. Moreover, the national transit system seemingly provides one crucial condition: citizens use the train and cycling systems in an integrated manner, with combined bicycle-train transport recently demonstrating strong growth. Relatively little is known about bicycle-train users, i.e. the people who combine the bicycle and the train in a single trip. In this paper, we investigate their profiles and travel choices, in terms of the modes they choose for access and egress travel, their choice of stations, and their choice of type of bicycles. Studying this specific group can add to our understanding of the role of the train system in the success of cycling in the Netherlands, in turn helping improve policy transfer to metropolitan areas in other countries. In 2017, in cooperation with the Dutch National Railways, researchers surveyed a sample of train travellers, ultimately resulting in more than 3000 completed questionnaires. Descriptive analyses revealed that, compared to train travellers who do not or rarely cycle to/from train stations, bicycle-train users are on average more likely to be young people who are engaged in full-time employment or entrepreneurs, commute to work and hold university degrees. As for their cycling behaviour, bicycle-train travellers use bicycles much more often on the home-end of train trips than on the activity-end. Furthermore, bicycle-train travellers infrequently use suburban stations on the home-end, preferring large stations in the centres of major cities instead. For those who use bicycles, shared bicycles claim a considerable share on the activity-end of a train trip.
From empirical to theoretical models of light response curves - linking photosynthetic and metabolic acclimation
Authors: Herrmann Helena A. , Schwartz Jean-Marc , Johnson Giles N. 
Published: October 2019

AbstractLight response curves (LRCs) describe how the rate of photosynthesis varies as a function of light. They provide information on the maximum photosynthetic capacity, quantum yield, light compensation point and leaf radiation use efficiency of leaves. Light response curves are widely used to capture photosynthetic phenotypes in response to changing environmental conditions. However, models describing these are predominantly empirical and do not attempt to explain behaviour at a mechanistic level. Here, we use modelling to understand the metabolic changes required for photosynthetic acclimation to changing environmental conditions. Using a simple kinetic model, we predicted LRCs across the physiological temperature range ofArabidopsis thalianaand confirm these using experimental data. We use our validated metabolic model to make novel predictions about the metabolic changes of temperature acclimation. We demonstrate that NADPH utilization are enhanced in warm-acclimated plants, whereas both NADPH and CO2utilization is enhanced in cold-acclimated plants. We demonstrate how different metabolic acclimation strategies may lead to the same photosynthetic response across environmental change. We further identify that certain metabolic acclimation strategies, such as NADPH utilization, are only triggered when plants are moved beyond a threshold high or low temperature.
Deep Insights into Convolutional Networks for Video Recognition
Authors: Feichtenhofer Christoph , Pinz Axel , Wildes Richard P. , Zisserman Andrew 
Published: October 2019

Abstract
As the success of deep models has led to their deployment in all areas of computer vision, it is increasingly important to understand how these representations work and what they are capturing. In this paper, we shed light on deep spatiotemporal representations by visualizing the internal representation of models that have been trained to recognize actions in video. We visualize multiple two-stream architectures to show that local detectors for appearance and motion objects arise to form distributed representations for recognizing human actions. Key observations include the following. First, cross-stream fusion enables the learning of true spatiotemporal features rather than simply separate appearance and motion features. Second, the networks can learn local representations that are highly class specific, but also generic representations that can serve a range of classes. Third, throughout the hierarchy of the network, features become more abstract and show increasing invariance to aspects of the data that are unimportant to desired distinctions (e.g. motion patterns across various speeds). Fourth, visualizations can be used not only to shed light on learned representations, but also to reveal idiosyncrasies of training data and to explain failure cases of the system.
How do body-worn cameras affect the amount and makeup of police-initiated activities? A randomized controlled trial in Milwaukee, Wisconsin
Authors: Lawrence Daniel S. , Peterson Bryce E. 
Published: October 2019

AbstractObjectivesExamine how the amount and makeup of police-initiated activities changed after the introduction of body-worn cameras (BWCs).MethodsFrom May 21 to November 22, 2016, patrol officers and sergeants from the Milwaukee Police Department were involved in a randomized controlled trial. Through a stratified random sampling procedure, half the officers (n= 252) were assigned BWCs, while officers from the control group (n= 252) continued business as usual. The counts of proactive activities, which included a total count of self-initiated events, as well as traffic stops, business checks, subject stops, and park and walks, were examined using random-effects negative binominal panel regression analyses. The models included a unique measure of contamination to assess its impact on officers’ proactivity.ResultsBWCs had no impact on the total amount of officer-initiated activities, traffic stops, or business checks. Officers with BWCs conducted approximately 8% fewer subject stops and 23% more park and walks than those in the control group. In all models, contamination levels were significantly, positively associated with the number of proactive activities that were conducted; however, the size of this effect was very small.ConclusionsResults suggest that BWC-wearing officers may be less likely to engage in proactive activities that are highly discretionary and that could potentially lead to confrontations with community members. As departments continue to develop BWC programs or fine-tune their existing BWC policies, more research is needed to understand the degree to which BWCs affect officers’ behaviors and interactions with the public.
Age-related alterations of articular cartilage in pituitary adenylate cyclase–activating polypeptide (PACAP) gene–deficient mice
Authors: Szegeczki Vince , Bauer Balázs , Jüngling Adél , Fülöp Balázs Daniel , Vágó Judit , Perényi Helga , Tarantini Stefano , Tamás Andrea , Zákány Róza , Reglődi Dóra , Juhász Tamás 
Published: October 2019

Abstract
Pituitary adenylate cyclase activating polypeptide (PACAP) is an evolutionarly conserved neuropeptide which is produced by various neuronal and non-neuronal cells, including cartilage and bone cells. PACAP has trophic functions in tissue development, and it also plays a role in cellular and tissue aging. PACAP takes part in the regulation of chondrogenesis, which prevents insufficient cartilage formation caused by oxidative and mechanical stress. PACAP knockout (KO) mice have been shown to display early aging signs affecting several organs. In the present work, we investigated articular cartilage of knee joints in young and aged wild-type (WT) and PACAP KO mice. A significant increase in the thickness of articular cartilage was detected in aged PACAP gene–deficient mice. Amongst PACAP receptors, dominantly PAC1 receptor was expressed in WT knee joints and a remarkable decrease was found in aged PACAP KO mice. Expression of PKA-regulated transcription factors, Sox5, Sox9 and CREB, decreased both in young and aged gene deficient mice, while Sox6, collagen type II and aggrecan expressions were elevated in young but were reduced in aged PACAP KO animals. Increased expression of hyaluronan (HA) synthases and HA-binding proteins was detected parallel with an elevated presence of HA in aged PACAP KO mice. Expression of bone related collagens (I and X) was augmented in young and aged animals. These results suggest that loss of PACAP signaling results in dysregulation of cartilage matrix composition and may transform articular cartilage in a way that it becomes more prone to degenerate.
The Kinetics of Primary Alpha Plate Growth in Titanium Alloys
Authors: Ackerman Abigail K. , Knowles Alexander J. , Gardner Hazel M. , Németh André A. N. , Bantounas Ioannis , Radecka Anna , Moody Michael P. , Bagot Paul A. J. , Reed Roger C. , Rugg David , Dye David 
Published: October 2019

Abstract
The kinetics of primary α-Ti colony/Widmanstätten plate growth from the β are examined in Ti-6246, comparing a simple quasi-analytic model to experiment. The plate growth velocity depends sensitively both on the diffusivity D(T) of the rate-limiting species and on the supersaturation around the growing plate. These result in a maxima in growth velocity around 40 K below the transus, once sufficient supersaturation is available to drive the plate growth. In Ti-6246, the plate growth velocity was found to be around 0.32 μm min−1 at 850 °C, which was in good agreement with the model prediction of 0.36 μm min−1. The solute field around the growing plates, and the plate thickness, was found to be quite variable, due to the intergrowth of plates and soft impingement. This solute field was found to extend to up to 30 nm, and the interface concentration in the β was found to be around 6.4 at. pct Mo. It was found that the increasing O content from 500 to 1500 wppm will have minimal effect on the plate lengths expected during continuous cooling; in contrast, Mo approximately doubles the plate lengths obtained for every 2 wt pct Mo reduction. Alloys using V as the β stabilizer instead of Mo are expected to have much faster plate growth kinetics at nominally equivalent V contents. These findings will provide a useful tool for the integrated design of alloys and process routes to achieve tailored microstructures.
Emerging Apprenticeship Practitioner Roles in England: Conceptualising the Subaltern Educator
Authors: Esmond Bill 
Published: October 2019

AbstractTVET educator roles and identities vary internationally, and are subject to repositioning, for example as the relative significance of institutions and the workplace change within national systems. In English apprenticeships, a key position has long been occupied by competence assessors, whose non-teaching role has related uneasily to those of professional educators. Following the introduction of new apprenticeship standards, former assessors are increasingly being allocated training responsibilities, raising issues about the expertise, identities and professional formation both of these emerging practitioners and of vocational educators in general. A qualitative study of assessors who have assumed greater training responsibilities examined these issues through individual and small-group interviews. Participant accounts of diverse and contested practices and environments suggested a need to conceptualise their roles in ways that draw upon but go beyond accounts of professionalism and occupational expertise developed at earlier stages. Drawing on Gramsci, the concept of the subaltern educator is put forward to characterise the complex position of these staff in the current climate of further education, the need for enhanced, rather than diminished, professional formation and wider possibilities for professional enhancement at a time of uncertainty for all vocational educators.
A Rare Case of Primary Gastric Melanoma
Authors: Wiewiora Maciej , Steplewska Katrzyna , Piecuch Jerzy Z. , Piecuch Jerzy 
Published: October 2019

Abstract
Primary melanomas in the gastrointestinal tract are extremely rare, with an estimated prevalence of 0.5 to 1 case per million. Primary mucosal melanomas of gastric origin account for approximately 1–3% of all primary melanomas in the gastrointestinal tract. We present the case of a patient who underwent surgery due to primary gastric melanoma.
Electrochemical Characterization of Low-Temperature Direct Ethanol Fuel Cells using Direct and Alternate Current Methods
Authors: Wnuk Paweł , Jurczakowski Rafał , Lewera Adam 
Published: October 2019

AbstractHere, we report for the first time the results of systematic characterization of a low-temperature polymer electrolyte membrane direct ethanol fuel cell using DC and AC electrochemical methods. Model catalysts (carbon supported Pt nanoparticles) painted on carbon paper are used as anode and cathode. Influence of physical parameters, such as cell temperature, current density, and ethanol concentration, and anode fuel flow rate on overall cell impedance is studied. Analysis of the obtained impedance spectra in connection with DC measurements allows us to comment on cell properties and to separate different contributions to the overall cell polarization. Our results suggest that the cell impedance is dominated by anode faradaic impedance, with a small or negligible contribution from cathode faradaic impedance. The anode impedance depends strongly on current density and cell temperature, but is not significantly influenced by ethanol concentration. Presence of anode mass-transfer impedance, even when ethanol was fed to the cell in high excess, is confirmed. Based on the results, we conclude that changes in ethanol electro-oxidation mechanism might manifest themselves on the impedance spectra in the low-frequency inductive loop. Nonetheless, further studies involving equivalent circuit modelling are needed to determine the exact influence of the cell parameters on the anode kinetics.
Photoinduced K+ Intercalation into MoO3/FTO Photoanode—the Impact on the Photoelectrochemical Performance
Authors: Szkoda M. , Trzciński K. , Łapiński M. , Lisowska-Oleksiak A. 
Published: October 2019

AbstractIn this work, thin layers of MoO3were tested as potential photoanodes for water splitting. The influence of photointercalation of alkali metal cation (K+) into the MoO3structure on the photoelectrochemical properties of the molybdenum trioxide films was investigated for the first time. MoO3thin films were synthesized via thermal annealing of thin, metallic Mo films deposited onto the FTO substrate using a magnetron sputtering system. The Tauc and Mott–Schottky plots analysis were performed in order to determine the energy bands position of the investigated material. The photointercalation effect of K+on photoelectrochemical properties of FTO/MoO3photoanodes was studied using electrochemical techniques performed under simulated solar light illumination. It was proven that pristine MoO3layers cannot act as effective photoanodes for water splitting due to the utilization of the photoexcited electrons in the intercalation process. The photochromic phenomenon related to Mo6+centers reduction, and K+intercalation occurs at a potential range in which the photoanode exhibits photoelectrochemical activity towards water photooxidation.
A Survey of Oncologists’ Perceptions and Opinions Regarding the Use of Granulocyte Colony-Stimulating Factors
Authors: Hawkins Alicia , Murphy Alysa , McNamara Michelle , Gawade Prasad L. , Belani Rajesh , Kelsh Michael A. 
Published: October 2019

Abstract
The purpose of the study is to describe oncologists’ perceptions and opinions about patient eligibility, guidelines, and barriers for use of granulocyte colony-stimulating factor (G-CSF), overall and stratified by their affiliation with the Oncology Care Model (OCM). In May 2018, we invited and recruited practicing US oncologists from a national database for an online survey. Level of agreement was identified using a seven-point scale, ranging from strongly disagree to strongly agree. Of 200 participating oncologists, 70 were OCM-affiliated. Overall, 65% of oncologists agreed or strongly agreed that all patients at high risk of febrile neutropenia (FN) should receive prophylactic G-CSF, and half agreed or strongly agreed that benefits of G-CSF outweigh the potential adverse effects. The most common barriers to G-CSF use for patients at high risk of FN included patient refusal (37.1% of OCM-affiliated oncologists vs. 21.5% of non-OCM-affiliated oncologists), not on protocol/not supported by guidelines (32.9% vs. 23.1%), lack of reimbursement to practice (30.0% vs. 15.4%), and concerns about insurance coverage (22.9% vs. 26.9%). More OCM-affiliated oncologists reported that their practices offer and strongly encourage adherence to a specific protocol for G-CSF use (49.2%) versus non-OCM oncologists (31.3%). Despite recommendations from national guidelines and strong evidence from randomized, controlled clinical trials, only two thirds of oncologists agree or strongly agree that all patients at high risk of FN should receive primary G-CSF prophylaxis. Decisions about G-CSF prophylaxis may be affected by factors other than risk of FN, such as patient choice, practice protocols/guidelines, lack of reimbursement, and insurance coverage.
Investigations on effectiveness of wheat and rice straw mulches on moisture retention in potato crop (Solanum tuberosum L.)
Authors: Goel Lalit , Shankar Vijay , Sharma R. K. 
Published: October 2019

Abstract

Purpose
Mulching is a practice recommended for soil moisture conservation in potato. The wheat straw and rice straw obtained as major crop residues were used as mulching materials to compare their effectiveness for soil moisture retention in potato crop.

Methods
The field experiments were conducted in a randomized complete block design replicated thrice with three treatments viz. plots incorporated with wheat straw mulch, rice straw mulch at the rate of 10 tonnes per hectare each and no mulch serving as control. The soil moisture was determined using a soil moisture probe and data were recorded daily at 10 cm, 20 cm and 30 cm soil depths. The Field Emission Scanning Electron Microscopy was used to investigate moisture retention characteristics of the mulch materials.

Results
Soil moisture retention varied as wheat straw mulch > rice straw mulch > no mulch at 10 cm, 20 cm and 30 cm soil depths, respectively. Highest moisture retention in wheat straw mulch at 10 cm depth is attributed to better moisture absorption ability of wheat straw in comparison to rice straw. Field Emission Scanning Electron Microscope images indicated the presence of smaller sized micro tubes in wheat straw than rice straw, which resulted in more water retention, thereby substantiating the findings of the study.

Conclusion
Wheat straw mulch is more effective than rice straw mulch for shallow rooted crops like potato, due to better moisture absorption and retention in upper soil layer.
Effect of different initial C/N ratio of cow manure and straw on microbial quality of compost
Authors: Macias-Corral M. A. , Cueto-Wong J. A. , Morán-Martínez J. , Reynoso-Cuevas L. 
Published: October 2019

Abstract

Purpose
The effect of different initial carbon to nitrogen (C/N) ratios on the prevalence of Salmonella spp., fecal coliforms and helminth eggs over composts produced from several mixtures of maize straw (S) and dairy manure (M) was investigated.

Methods
Four C/N ratios (21, 22, 27, and 38) were evaluated, including one with manure only (C/N 21). The composting process was performed under field conditions in northern Mexico.

Results
The process lasted 51 days; Salmonella spp. was reduced 1–2 log (> 94%) in most treatments, except for the C/N ratio of 27 which achieved < 1 log reduction (about 35%). Fecal coliforms elimination was 3–4 log (> 99%) in all treatments while helminth eggs achieved < 1 log (72–87%, depending on treatment). In this study, the mixture with initial C/N ratio of 22 (25% S + 75% M), which had the lowest amount of straw, resulted in the highest elimination of Salmonella spp., fecal coliforms, and helminth eggs. This mix complied with current Mexican sanitary regulations for compost use. The composts produced from the other C/N ratios complied only with the limits for one or two of the microorganisms that were analyzed.

Conclusions
The initial C/N ratios in compost from straw and manure influences microbial reduction. The final C/N of the mixes ranged from 14 to 16, indicative of stable compost. Compared to fecal coliforms, Salmonella spp. and helminth eggs were more resilient.