bluuebunny/crossref_metadata_2025_split huggingface dataset:
Graphene Oxide and Vermiculite Clay Combinations to Produce Enhanced Flame Retardant Polypropylene Composite with Low Magnesium Hydroxide Loading
Authors: Amador‐Noya José Victor , Sánchez‐Valdes Saul , Ramos‐de Valle Luis Francisco , Valle‐Orta Maiby , Borjas‐Ramos José Javier , Beltrán‐Ramírez Flora Itzel , Valera‐Zaragoza Mario , Rodríguez‐González José Alberto , Flores‐Flores Rodolfo , Saldivar‐Guerrero Ruben , Ibarra‐Alonso María Cristina , Cabrera‐Álvarez Edgar Nazareo 
Published: May 2020

AbstractThe effect of graphene oxide (GO) functionalized with 3‐amino‐propyl‐triethoxy‐silane(APTS), organo‐vermiculite(OVMT), and magnesium hydroxide (MH) combinations on mechanical, thermal, and flame retardant (FR) properties of polypropylene (PP) was studied. GO was obtained via a slight modification of the Hummers method and then chemically surface functionalized with APTS. VMT clay was modified with maleic anhydride (MA) via a chemical reaction with acetic acid to increase its inter‐laminar spacing. The results of Fourier‐transform infrared analysis, X‐ray diffraction, and transmission electron microscopy demonstrated that APTS had been successfully attached to the GO and that VMT was modified with MA. Subsequently, each functionalized filler was incorporated in combination with MH to the flame‐retardant‐polypropylene system. The performance of PP composites with each filler as well as with their combinations, including a reduced “30 wt% of MH” were compared with the reference PP composite with 55 wt% of MH as the only FR additive. PP grafted with MA (PP‐gMA) and PP grafted with amine‐alcohol(PP‐gDMAE) were used as compatibilizer agents between each filler and the polymer matrix. The results obtained confirmed a better mechanical and FR performance when using PP‐gDMAE. Composites with 30 wt% MH combined with very low contents of GO (0.5 and 1.0 wt%) showed improved FR properties, similar to the reference sample, with an evident reduction in peak of the heat release rate (pHRR) and total heat release and increased limiting oxygen index (LOI) values. The combination of MH and GO showed the best FR and mechanical properties: LOI of 22.5% and pHRR of 540 kW/m2 which were very similar to the reference sample. In addition, PP‐gDMAE improved the OVMT exfoliation with a slight increase in the intergallery spacing but OVMT and modified GO combinations did not significantly improve the FR and mechanical properties compared with the effect of MH and modified GO combinations. The combination of MH and modified GO makes it possible to reduce the total MH filler content from 55 to 30 wt% to accomplish the FR requirements and with enhanced mechanical properties. This filler combination promoted the formation of a continuous, intact residual char layer on the PP surface, which acts as an insulating barrier to protect the base material. These filler combinations offer an option to meet the FR properties using halogen‐free FR with better mechanical properties.
Mixed culture of Lactococcus lactis and Kluyveromyces marxianus isolated from kefir grains for pollutants load removal from Jebel Chakir leachate
Authors: Cherni Yasmin , Botta Cristian , Kasmi Mariam , Franciosa Irene , Cocolin Luca , Chatti Abdelwaheb , Trabelsi Ismail , Elleuch Lobna 
Published: May 2020

AbstractThe wastewater from the dumping site usually contains high pollutant levels. Biological process and physico‐chemical treatments are among several technologies for wastewater treatment. Using microorganisms in the treatment of landfill leachate is an emerging research issue. Furthermore, bioremediation is a feasible approach for pollutants removal from landfill leachate which would provide an efficient way to resolve the issue of landfill leachate. In this study, the performance of yeast and bacteria isolated from kefir grains was assessed for landfill leachate treatment. Kefir grains microbial composition was evaluated by molecular approaches (Rep‐PCR and 16S rRNA gene sequencing). The obtained outcomes denoted that high concentrations of lactic acid bacteria and yeast populations (over 107 CFU/ml) were found in the kefir grains and were essentially composed of Lactococcus lactis, Lactobaccillus kefirien, bacillus sp., L. lactis, and Kluyveromyces marxianus. The co‐culture with 1% of inoculum size was demonstrated as the most efficient in the degradation of different contaminants. The overall abatement rate of chemical oxygen demand (COD), ammonium nitrogen (

), and salinity were 75.8%, 85.9%, and 75.13%, respectively. The bioremediation process resulted in up of 75% removal efficiency of Ni and Cd, and a 73.45%, 68.53%, and a 58.17% removal rates of Cu, Pb, and Fe, respectively. The research findings indicate the performance of L. lactis and K. marxianus co‐culture isolated from kefir grains for the bioremediation of LFL.Practitioner Points

Isolation and identification of microorganisms from kefir grains was carried out.

Biological treatment of LFL using monoculture of (Lactoccocus lactis; Kluyveromyces marxianus) and co‐culture (5% of L. lactis and 5% K. marxianus) has been performed.

Biological treatment using co‐culture strain is an effective approach to remove organic matter,

and heavy metals.
NET-VISA from Cradle to Adulthood. A Machine-Learning Tool for Seismo-Acoustic Automatic Association
Authors: Le Bras Ronan , Arora Nimar , Kushida Noriyuki , Mialle Pierrick , Bondár Istvan , Tomuta Elena , Alamneh Fekadu Kebede , Feitio Paulino , Villarroel Marcela , Vera Beatriz , Sudakov Alexander , Laban Shaban , Nippress Stuart , Bowers David , Russell Stuart , Taylor Tammy 
Published: May 2020

AbstractA research concept that was first presented on a poster at a Comprehensive Nuclear-Test-Ban Treaty (CTBT) conference in 2009 has resulted in a fully-fledged operational software product named NET-VISA (Network Processing Vertically Integrated Seismic Analysis), which performs the Network Processing step of the automatic processing at the International Data Centre (IDC). It has become one of the tools of the waveform analysts to review and improve the IDC standard automatic third Standard Events List (SEL3) bulletin and produce the Reviewed Events Bulletin (REB), the only global seismo-acoustic bulletin. The basic scientific concepts imbedded into NET-VISA are briefly summarized in this paper but the emphasis is on the process of adopting, developing, adapting, testing, and operationalizing the initial prototype. Extensive off-line testing has shown that one of the expected benefits of NET-VISA was the significant reduction in missed events rate compared to the current Network Processing software, Global Association (GA). NET-VISA also finds some events previously missed by manual review. Starting in July 2017, and as of January 2020, NET-VISA generates an automatic bulletin, called VSEL3, in parallel to SEL3, which is also used by analysts since January 2018. Tracing the origin of the REB events has confirmed the significant reduction in missed events when complementary VSEL3 events are reviewed in addition to the SEL3 events. If sufficient confidence is established, NET-VISA has the potential to replace GA in producing the standard automatic SEL3 bulletin.
Spielerische Ansätze in Prävention und Gesundheitsförderung: Serious Games und Gamification
Authors: Tolks Daniel , Lampert Claudia , Dadaczynski Kevin , Maslon Eveline , Paulus Peter , Sailer Michael 
Published: May 2020

ZusammenfassungDigitale Spieleanwendungen können im Bereich Prävention und Gesundheitsförderung eingesetzt werden, um etwa gesundheitsrelevante Informationen zu vermitteln oder Verhaltensänderungen zu evozieren. Dabei sind die beiden relevantesten spielerischen Ansätze Serious Games (Spiele mit ernsthaftem Hintergrund) und Gamification (Anwendung spieltypischer Elemente in spielfremden Kontexten). Der Begriff Serious Games umschreibt Spiele, die eingesetzt werden, um ernsthafte Inhalte, wie zum Beispiel gesundheitliche Themen, zu vermitteln. Bei Gamification werden Spieldesignelemente, wie beispielsweise Punkte, Bestenlisten, Auszeichnungen, Profilgestaltung und Teamevents, eingesetzt, um eine Motivations- und Leistungssteigerung etwa in Lernumgebungen zu bewirken. Der vorliegende narrative Beitrag beleuchtet die Wirkungsweise und Studienlage sowie die Vor- und Nachteile spielerischer Anwendungen in der Prävention und Gesundheitsförderung und führt einige dieser Anwendungen exemplarisch auf.Serious Games und Gamification zeigen in der Prävention und Gesundheitsförderung ein großes Potenzial. Insbesondere bei schwer erreichbaren und desinteressierten Zielgruppen kann ein direkter Bezug zu deren Lebenswelt hergestellt werden. Grundlagen für den wirkungsvollen Einsatz spielerischer Ansätze in der Arbeit und Ausbildung von Gesundheitsberufen sind die bereits hohe Vertrautheit mit dem Medium Spiel sowie die direkte Adressierung der psychologischen Grundbedürfnisse zur Steigerung der Motivation.
Ein strukturierter Versorgungspfad von der Pädiatrie in die Erwachsenenmedizin für Jugendliche und junge Erwachsene mit einer seltenen Erkrankung
Authors: Grasemann Corinna , Matar Nora , Bauer Jens , Manka Eva , Mundlos Christine , Krude Heiko , Grüters Annette , Hoffmann Georg F. , Choukair Daniela , Burgard Peter 
Published: May 2020

ZusammenfassungDie erfolgreiche Organisation und Umsetzung des Übergangs von Jugendlichen und jungen Erwachsenen mit einer chronischen seltenen Erkrankung aus der Pädiatrie in eine Versorgungsform (Transition) und Versorgungsstruktur (Transfer) der Erwachsenenmedizin ist eine wichtige Aufgabe im dezentral aufgebauten deutschen Gesundheitssystem. Ein mittlerweile in der Praxis erprobtes Programm stellt der strukturierte Versorgungspfad des vom Innovationsfonds des gemeinsamen Bundesausschuss (G‑BA) geförderten Konsortiums TRANSLATE-NAMSE dar (Förderkennzeichen 01NVF16024 TRANSLATE-NAMSE). Grundlage des Übergangs in diesem Programm ist der qualitätsgesicherte Informations- und Kompetenztransfer vom pädiatrischen Behandlungsteam zum adoleszenten Patienten, sowie zur neuen Versorgungseinrichtung. Basierend auf einer strukturierten Epikrise und Erhebung des individuellen Beratungsbedarfs erfolgt, ab dem Alter von 16 Jahren, die strukturierte Transitionsschulung des Patienten durch den Pädiater. Nach erfolgreich absolvierten Transfersprechstunden, gemeinsam mit Vertretern der bisherigen pädiatrischen und der zukünftigen erwachsenenmedizinischen Versorgungseinrichtungen, mündet der Prozess in die Übergabe aller notwendigen medizinischen Unterlagen an den Patienten und den Weiterbehandler sowie den Wechsel des Patienten in die neue Versorgungseinrichtung. Eine abschließende Evaluation des Projekts ist für Herbst 2020 geplant.
Evidence for systematic autopsies in COVID-19 positive deceased
Authors: Fitzek A. , Sperhake J. , Edler C. , Schröder A. S. , Heinemann A. , Heinrich F. , Ron A. , Mushumba H. , Lütgehetmann M. , Püschel K. 
Published: May 2020

AbstractForensic medicine and pathology involve specific health risks, whereby health workers are dealing with microorganisms, cells or parasites, which are referred to as biological agents. Biological agents are divided into four categories according to § 3 of the Biological Agents Ordinance. The newly identified coronavirus, severe acute respiratory syndrome, coronavirus 2 (SARS-CoV-2) that has spread rapidly around the world is placed into category 3 of the Biological Agents Ordinance, meaning pathogens that can cause serious illnesses in humans and may pose a risk to workers. The Robert Koch Institute, the German government’s central scientific institution in the field of biomedicine issued the announcement, that aerosol-producing measures (including autopsies) of SARS-CoV‑2 infected bodies should be avoided, despite the fact that autopsies are an important source of understanding the pathomorphological course of new diseases. The first German case of death due to a proven SARS-CoV‑2 infection is presented with global multifocal reticular consolidation in the post-mortem computed tomography (CT) scan, a macroscopic and microscopic viral pneumonia and viral RNA of SARS-CoV‑2 in pharyngeal mucosa and lung tissue.
Development of CDX-527: a bispecific antibody combining PD-1 blockade and CD27 costimulation for cancer immunotherapy
Authors: Vitale Laura A. , He Li-Zhen , Thomas Lawrence J. , Wasiuk Anna , O’Neill Thomas , Widger Jenifer , Crocker Andrea , Mills-Chen Laura , Forsberg Eric , Weidlick Jeffrey , Patterson Colleen , Hammond Russell A. , Boyer James , Sisson Crystal , Alvarado Diego , Goldstein Joel , Marsh Henry C. , Keler Tibor 
Published: May 2020

AbstractCD27 is a costimulatory molecule that provides a complementary target to the PD-1/PD-L1 checkpoint axis on T cells. Combining a CD27 agonist antibody with PD-1/PD-L1 blockade has shown synergistic antitumor activity in preclinical models, which led to clinical studies of the combination in cancer patients. We theorized that coupling CD27 costimulation with PD-1/PD-L1 blockade in a bispecific antibody (BsAb) may provide greater immune activating properties than combining the individual mAbs due to enhanced CD27 activation by cross-linking through PD-L1 and Fc receptors. To test this approach, we developed CDX-527, a tetravalent PD-L1xCD27 IgG1-scFv BsAb. CDX-527 potently inhibits PD-1 signaling and induces CD27-mediated T cell costimulation through PD-L1 cross-linking. In mixed lymphocyte reaction assays, CDX-527 is more potent than the combination of the parental antibodies, suggesting that cross-linking through both Fc receptors and PD-L1 results in enhanced CD27 agonist activity. CDX-527 was shown to mediate effector function against tumor cells overexpressing either CD27 or PD-L1. In human CD27 transgenic mice, we observed that antigen-specific T cell responses to a vaccine are greatly enhanced with a surrogate PD-L1xCD27 BsAb. Furthermore, the BsAb exhibits greater antitumor activity than the combination of the parental antibodies in a syngeneic lymphoma model. A pilot study of CDX-527 in cynomolgus macaques confirmed a mAb-like pharmacokinetic profile without noted toxicities. These studies demonstrate that CDX-527 effectively combines PD-1 blockade and CD27 costimulation into one molecule that is more potent than combination of the parental antibodies providing the rationale to advance this BsAb toward clinical studies in cancer patients.
Antitumor efficacy of BAFF-R targeting CAR T cells manufactured under clinic-ready conditions
Authors: Dong Zhenyuan , Cheng Wesley A. , Smith D. Lynne , Huang Brian , Zhang Tiantian , Chang Wen-Chung , Wang Xiuli , Forman Stephen J. , Kwak Larry W. , Qin Hong 
Published: May 2020

AbstractB-cell malignancies can potentially be cured by CD19 chimeric antigen receptor (CAR) T-cell therapy. Although clinical response rates can be up to 93% in acute lymphoblastic leukemia, treatment-related antigen loss and lack of therapeutic persistence contribute to disease relapse. These shortcomings of current CAR T-cell therapy indicate the need for biologically relevant target selection and for improving the efficacy and persistence of the CAR T cells, which we have addressed by developing a novel B-cell activating factor receptor (BAFF-R) CAR T-cell therapy with improved therapeutic persistence. BAFF-R is a B-cell survival receptor and highly expressed in B-cell malignancies. We developed a prototype CAR T cell that efficiently and specifically eliminated BAFF-R expressing human B-cell tumors in several xenogeneic mouse models, including models of CD19 antigen loss. We proceeded with translational development and validation of BAFF-R CAR T cells produced under current good manufacturing practices (cGMP). cGMP-grade BAFF-R CAR T cells underwent in vitro and in vivo validation in established models to confirm that the potency and efficacy of our original research modeling was replicated. Food and Drug Administration required release testing was performed to ensure our BAFF-R CAR T cells meet specifications for new drug products. Completing and exceeding these requirements, the data fully support the initiation of a first-in-human Phase 1 trial for BAFF-R-positive relapsed/refractory (r/r) B-ALL.
Energy efficiency of respiration in mature and newborn reindeer
Authors: Solberg Simon Birger Byremo , Kjelstrup Signe , Magnanelli Elisa , Kizilova Natalya , Barroso Iratxe Lorea Casado , Acquarone Mario , Folkow Lars P. 
Published: May 2020

AbstractReindeer (Rangifer tarandus) have evolved elaborate nasal turbinate structures that are perfused via a complex vascular network. These are subject to thermoregulatory control, shifting between heat conservation and dissipation, according to the animal’s needs. The three-dimensional design of the turbinate structures is essential in the sense that they determine the efficiency with which heat and water are transferred between the structure and the respired air. The turbinates have already a relatively large surface area at birth, but the structures have yet not reached the complexity of the mature animal. The aim of this study was to elucidate the structure–function relationship of the heat exchange process. We have used morphometric and physiological data from newborn reindeer calves to construct a thermodynamic model for respiratory heat and water exchange and present novel results for the simulated respiratory energy losses of calves in the cold. While the mature reindeer effectively conserves heat and water through nasal counter-current heat exchange, the nose of the calf has not yet attained a similar efficiency. We speculate that this is probably related to structure-size limitations and more favourable climate conditions during early life. The fully developed structure–function relationship may serve as inspiration for engineering design. Simulations of different extents of mucosal vascularization suggest that the abundance and pattern of perfusion of veins in the reindeer nasal mucosa may contribute to the control of temperature profiles, such that nasal cavity tissue is sufficiently warm, but not excessively so, keeping heat dissipation within limits.
Long-term safety and efficacy of daclizumab beta in relapsing–remitting multiple sclerosis: 6-year results from the SELECTED open-label extension study
Authors: Gold Ralf , Radue Ernst-Wilhelm , Giovannoni Gavin , Selmaj Krzysztof , Havrdova Eva Kubala , Montalban Xavier , Stefoski Dusan , Sprenger Till , Robinson Randy R. , Fam Sami , Smith Jonathan , Chalkias Spyros , Giannattasio Giorgio , Lima Gabriel , Castro-Borrero Wanda 
Published: May 2020

Abstract
Objective
SELECTED, an open-label extension study, evaluated daclizumab beta treatment for up to 6 years in participants with relapsing multiple sclerosis who completed the randomized SELECT/SELECTION studies. We report final results of SELECTED.

Methods
Eligible participants who completed 1–2 years of daclizumab beta treatment in SELECT/SELECTION received daclizumab beta 150 mg subcutaneously every 4 weeks for up to 6 years in SELECTED. Safety assessments were evaluated for the SELECTED treatment period; efficacy data were evaluated from first dose of daclizumab beta in SELECT/SELECTION.

Results
Ninety percent (410/455) of participants who completed treatment in SELECTION enrolled in SELECTED. Within SELECTED, 69% of participants received daclizumab beta for > 3 years, 39% for > 4 years, and 9% for > 5 years; 87% of participants experienced an adverse event and 26% a serious adverse event (excluding multiple sclerosis relapse). No deaths occurred. Overall, hepatic events were reported in 25% of participants; serious hepatic events in 2%. There were no confirmed cases of immune-mediated encephalitis. Based on weeks from the first daclizumab beta dose in SELECT/SELECTION, adjusted annualized relapse rate (95% confidence interval) for weeks 0–24 was 0.21 (0.16–0.29) and remained low on continued treatment. Overall incidence of 24-week confirmed disability progression was 17.4%. Mean numbers of new/newly enlarging T2 hyperintense lesions remained low; percentage change in whole brain volume decreased over time.

Conclusions
The effects of daclizumab beta on clinical and radiologic outcomes were sustained for up to ~ 8 years of treatment. No new safety concerns were identified in SELECTED.

Trial registration
Clinicaltrials.gov NCT01051349; first registered on January 15, 2010.
Pretty crowds are happy crowds: the influence of attractiveness on mood perception
Authors: Mertens Alica , Hepp Johanna , Voss Andreas , Hische Amelie 
Published: May 2020

AbstractEmpirical findings predominantly support a happiness superiority effect in visual search and emotion categorization paradigms and reveal that social cues, like sex and race, moderate this advantage. A more recent study showed that the facial attribute attractiveness also influences the accuracy and speed of emotion perception. In the current study, we investigated whether the influence of attractiveness on emotion perception translates into a more general evaluation of moods when more than one emotional target is presented. In two experiments, we used the mood-of-the-crowd (MoC) task to investigate whether attractive crowds are perceived more positively compared to less attractive crowds. The task was to decide whether an array of faces included more angry or more happy faces. Furthermore, we recorded gaze movements to test the assumption that fixations on happy expressions occur more often in attractive crowds. Thirty-four participants took part in experiment 1 as well as in experiment 2. In both experiments, crowds presenting attractive faces were judged as being happy more frequently whereas the reverse pattern was found for unattractive crowds of faces. Moreover, participants were faster and more accurate when evaluating attractive crowds containing more happy faces as well as when judging unattractive crowds composed of more angry expressions. Additionally, in experiment 1, there were more fixations on happy compared to angry expressions in attractive crowds. Overall, the present findings support the assumption that attractiveness moderates emotion perception.
Long-term outcomes following intraosseous basivertebral nerve ablation for the treatment of chronic low back pain: 5-year treatment arm results from a prospective randomized double-blind sham-controlled multi-center study
Authors: Fischgrund Jeffrey S. , Rhyne Alfred , Macadaeg Kevin , Moore Gregory , Kamrava Evish , Yeung Christopher , Truumees Eeric , Schaufele Michael , Yuan Philip , DePalma Michael , Anderson David Greg , Buxton Douglas , Reynolds James , Sikorsky Michael 
Published: May 2020

Abstract
Background
Damaged or degenerated vertebral endplates are a significant cause of vertebrogenic chronic low back pain (CLBP). Modic changes are one objective MRI biomarker for these patients. Prior data from the treatment arm of a sham-controlled, RCT showed maintenance of clinical improvements at 2 years following ablation of the basivertebral nerve (BVN). This study reports 5-year clinical outcomes.

Methods
In total, 117 US patients were treated successfully with BVN ablation. Patient-reported outcomes of ODI, VAS, postablation treatments, and patient satisfaction were collected at a minimum of 5-years following BVN ablation. Primary outcome was mean change in ODI. Comparisons between the postablation and baseline values were made using an analysis of covariance with alpha 0.05.

Results
Of the 117 US treated patients 100 (85%) were available for review with a mean follow-up of 6.4 years (5.4–7.8 years). Mean ODI score improved from 42.81 to 16.86 at 5-year follow-up, a reduction of 25.95 points (p < 0.001). Mean reduction in VAS pain score was 4.38 points (baseline of 6.74, p < 0.001). In total, 66% of patients reported a > 50% reduction in pain, 47% reported a > 75% reduction in pain, and 34% of patients reported complete pain resolution. Composite responder rate using thresholds of ≥ 15-point ODI and ≥ 2-point VAS for function and pain at 5 years was 75%.

Conclusion
CLBP patients treated with BVN ablation exhibit sustained clinical improvements in function and pain with high responder rates at a mean of 6.4 years following treatment. BVN ablation is a durable, minimally invasive treatment for vertebrogenic CLBP.