bluuebunny/crossref_metadata_2025_split huggingface dataset:
High Rates of Uncorrected Vision Conditions among Schoolchildren in Rural Queensland, Australia
Authors: Cox Rebecca A. , Read Scott A. , Hopkins Shelley , Wood Joanne M. 
Published: December 2020

SIGNIFICANCE
This study is the first to report high rates of uncorrected vision conditions among Australian secondary schoolchildren living in a rural area and to comment on the rate of eye examinations undertaken on Australian Indigenous children. Uncorrected vision problems that continue throughout the school years have significant implications for children's quality of life and education.

PURPOSE
This study aimed to investigate the prevalence of uncorrected vision conditions among Australian schoolchildren.

METHODS
Participants included 280 students from rural primary and secondary schools (aged 4 to 18 years), of whom 40% identified as being of Aboriginal and/or Torres Strait Islander descent (Indigenous). All participants underwent an eye examination including measurements of monocular distance and near visual acuity, assessment of accommodative and vergence function, stereoacuity, color vision, and cycloplegic autorefraction. A parental questionnaire was used to determine whether the child had previously had his/her eyes examined.

RESULTS
The overall prevalence of uncorrected vision conditions in this population was 35%. The odds of previously having had an eye examination were 2.3× higher for non-Indigenous compared with Indigenous children despite both groups exhibiting high rates of uncorrected vision conditions (Indigenous, 31 [29%]; non-Indigenous, 66 [40%]; χ2
1 = 3.24, P = .07). Of the children who had significant refractive error (Indigenous, 23 [21%]; non-Indigenous, 49 [30%]; χ2
1 = 2.70, P = .10), 82% were uncorrected, and only 39% of Indigenous children and 54% of non-Indigenous children had previously had an eye examination.

CONCLUSIONS
These findings suggest that high rates of uncorrected vision conditions are present among Australian primary and secondary schoolchildren from a rural area and highlight that Indigenous children are much less likely to have had an eye examination. Understanding factors that affect the rate of eye examinations and compliance with spectacle correction must be addressed given the potential impact of these vision conditions.
Indicators for Assessing the Quality of Refractive Error Care
Authors: Lee Ling , Burnett Anthea M. , D'Esposito Fabrizio , Fricke Tim , Nguyen Long Tien , Vuong Duong Anh , Nguyen Hien Thi Thu , Yu Mitasha , Nguyen Ngoc Viet My , Huynh Ly Phuong , Ho Suit May 
Published: December 2020

SIGNIFICANCE
Quality refractive error care is essential for reducing vision impairment. Quality indicators and standardized approaches for assessing the quality of refractive error care need to be established.

PURPOSE
This study aimed to develop a set of indicators for assessing the quality of refractive error care and test their applicability in a real-world setting using unannounced standardized patients (USPs).

METHODS
Patient outcomes and three quality of refractive error care (Q.REC) indicators (1, optimally prescribed spectacles; 2, adequately prescribed spectacles; 3, vector dioptric distance) were developed using existing literature, refraction training standards, and consulting educators. Twenty-one USPs with various refractive errors were trained to visit optical stores across Vietnam to have a refraction, observe techniques, and order spectacles. Spectacles were assessed against each Q.REC indicator and tested for associations with vision and comfort.

RESULTS
Overall, 44.1% (184/417) of spectacles provided good vision and comfort. Of the spectacles that met Q.REC indicators 1 and 2, 62.5 and 54.9%, respectively, provided both good vision and comfort. Optimally prescribed spectacles (indicator 1) were significantly more likely to provide good vision and comfort independently compared with spectacles that did not meet any indicator (good vision: 94.6 vs. 85.0%, P = .01; comfortable: 66.1 vs. 36.3%, P < .01). Adequately prescribed spectacles (indicator 2) were more likely to provide good comfort compared with spectacles not meeting any indicator (57.7 vs. 36.3%, P < .01); however, vision outcomes were not significantly different (85.9 vs. 85.0%, P = .90). Good vision was associated with a lower mean vector dioptric distance (P < .01) but not with comfort (P = .52).

CONCLUSIONS
The optimally prescribed spectacles indicator is a promising approach for assessing the quality of refractive error care without additional assessments of vision and comfort. Using USPs is a practical approach and could be used as a standardized method for evaluating the quality of refractive error care.
Fully autonomous mouse behavioral and optogenetic experiments in home-cage
Authors: Hao Yaoyao , Thomas Alyse M. , Li Nuo 
Published: December 2020

SummaryGoal-directed behaviors involve distributed brain networks. The small size of the mouse brain makes it amenable to manipulations of neural activity dispersed across brain areas, but existing optogenetic methods serially test a few brain regions at a time, which slows comprehensive mapping of distributed networks. Laborious operant conditioning training required for most experimental paradigms exacerbates this bottleneck. We present an autonomous workflow to survey the involvement of brain regions at scale during operant behaviors in mice. Naïve mice living in a home-cage system learned voluntary head-fixation (>1 hour/day) and performed difficult decision-making tasks, including contingency reversals, for 2 months without human supervision. We incorporated an optogenetic approach to manipulate activity in deep brain regions through intact skull during home-cage behavior. To demonstrate the utility of this approach, we tested dozens of mice in parallel unsupervised optogenetic experiments, revealing multiple regions in cortex, striatum, and superior colliculus involved in tactile decision-making.
Development of next-generation sequencing-based sterility test
Authors: Yue Jianhui , Chen Chao , Jing Xiaohuan , Ma Qiwang , Li Bo , Zhang Xi 
Published: December 2020

AbstractThe sterility testing methods described in pharmacopoeias require an incubation period of 14 days to obtain analysis results. An alternative method that can significantly shorten the detection time and improve the accuracy is in urgent need to meet the sterility testing requirements of regenerative medicine products with a short shelf life. In this study, we developed the next-generation sequencing-based sterility test (NGSST) based on sequencing and multiple displacement amplification. The NGSST can be finished within 48 hours with five steps including whole genome amplification, sequencing, alignment, sterility testing report, and microorganism identification. We use RPKM ratio to minorize the influence of environmental bacteria and determine its cutoff based AUC curve. The NGSST showed high sensitivity in reporting contaminates at 0.1 CFU in supernatant of biological product or 1 CFU in cell suspension. Furthermore, we identified microorganisms in 5 primary umbilical cord mesenchymal stem cell samples that were tested positive by BacT/ALERTR 3D. Overall, the NGSST can serve as a promising alternative for sterility testing of biological products.
ASIC3-dependent spinal cord nociceptive signaling in cutaneous pain induced by lysophosphatidyl-choline
Authors: Pidoux Ludivine , Delanoe Kevin , Lingueglia Eric , Deval Emmanuel 
Published: December 2020

ABSTRACTLysophosphatidyl-choline (LPC), a member of the phospholipid family, has recently emerged as an interesting new player in pain. It has been proposed to mediate pain through Acid-Sensing Ion Channel 3 (ASIC3), a pain-related channel mainly expressed in peripheral sensory neurons. LPC potentiates ASIC3 current evoked by mild acidifications, but can also activate the channel at physiological pH, and its local injection in rodents evokes ASIC3-dependent pain. We combine herein vivorecordings of spinal cord neuron activity with subcutaneous LPC injection to analyze the mechanism of action associated with the LPC-induced, ASIC3-dependent pain in peripheral and spinal cord neurons. We show that a single cutaneous injection of LPC exclusively affects the nociceptive pathway. It evokes an ASIC3-dependent short-term sensitization of nociceptive fibers that drives hyperexcitability of projecting neurons within the dorsal spinal cord without apparent central sensitization.
Reversion is most likely under high mutation supply, when compensatory mutations don’t fully restore fitness costs
Authors: Pennings Pleuni S. , Ogbunugafor C. Brandon , Hershberg Ruth 
Published: December 2020

AbstractAdaptive mutations are often associated with a fitness cost. These costs can be compensated for through the acquisition of additional mutations, or the adaptations can be lost through reversion in settings where they are no longer favored. While the dynamics of adaptation, reversion and compensation have been central features in several studies of microbial evolution, few studies have attempted to resolve the population genetics underlying how and when either compensation or reversion occur. Specifically, questions remain regarding how certain actors—the evolution of mutators and whether compensatory mutations alleviate costs fully or partially— may influence evolutionary dynamics of compensation and reversion. In this study, we attempt to explain findings from an experimental evolution study by utilizing computational and theoretical approaches towards a more refined understanding of how mutation rate and the fitness effects of compensatory mutations influence evolutionary dynamics. We find that high mutation rates increase the probability of reversion towards the wild type when compensation is only partial. However, the existence of even a single fully compensatory mutation is associated with a dramatically decreased probability of reversion to the wild type. These findings help to explain specific findings from experimental evolution, where compensation was observed in non-mutator strains, but reversion (sometimes with compensation) was observed in mutator strains, indicating that real-world compensatory mutations are often unable to fully alleviate the costs associated with resistance. Our findings emphasize the potential role of the supply and quality of mutations in crafting the evolution of antibiotic resistance, and more generally highlight the importance of population genetic context for explaining findings from experimental evolution.
Unusual mode of dimerization of retinitis pigmentosa-associated F220C rhodopsin
Authors: Khelashvili George , Pillai Anoop Narayana , Lee Joon , Pandey Kalpana , Payne Alexander M. , Siegel Zarek , Cuendet Michel A. , Lewis Tylor R. , Arshavsky Vadim Y. , Broichhagen Johannes , Levitz Joshua , Menon Anant K. 
Published: December 2020

AbstractMutations in the G protein-coupled receptor (GPCR) rhodopsin are a common cause of autosomal dominant retinitis pigmentosa, a blinding disease. Rhodopsin self-associates in the membrane, and the purified monomeric apo-protein opsin dimerizes in vitro as it transitions from detergent micelles to reconstitute into a lipid bilayer. We previously reported that the retinitis pigmentosa-linked F220C opsin mutant fails to dimerize in vitro, reconstituting as a monomer. Using fluorescence-based assays and molecular dynamics simulations we now report that whereas wildtype and F220C opsin display distinct dimerization propensities in vitro as previously shown, they both dimerize in the plasma membrane of HEK293 cells. Unexpectedly, molecular dynamics simulations show that F220C opsin forms an energetically favored dimer in the membrane when compared with the wild-type protein. The conformation of the F220C dimer is unique, with transmembrane helices 5 and 6 splayed apart, promoting widening of the intracellular vestibule of each protomer and influx of water into the protein interior. FRET experiments with SNAP-tagged wild-type and F220C opsin expressed in HEK293 cells are consistent with this conformational difference. We speculate that the unusual mode of dimerization of F220C opsin in the membrane may have physiological consequences.
Cortical layer-specific modulation of neuronal activity after sensory deprivation due to spinal cord injury
Authors: Zaforas Marta , Rosa Juliana M. , Alonso-Calviño Elena , Fernández-López Elena , Miguel-Quesada Claudia , Oliviero Antonio , Aguilar Juan 
Published: December 2020

ABSTRACTCortical areas have the capacity of large-scale reorganization following sensory deprivation. However, it remains unclear whether this phenomenon is a unique process that homogenously affects an entire deprived cortical region or it is suitable to changes depending on neuronal networks across distinct cortical layers. Here, we studied how local circuitries within each layer of the deprived cortex set the basis for neuroplastic changes after immediate sensory deprivation due to thoracic spinal cord injury (SCI) in anaesthetised rats.In vivoelectrophysiological recordings from deprived hindlimb somatosensory cortex showed that SCI induces layer-specific changes mediating evoked and spontaneous activity. In supragranular layers 2/3, sensory deprivation increased gamma oscillations and the ability of these neurons to initiate up-states during spontaneous activity, suggesting altered corticocortical network and/or intrinsic properties that may serve to maintain the excitability of the cortical column after deprivation. On the other hand, sensory deprivation enhanced infragranular layers’ ability to integrate evoked-sensory inputs leading to increased and faster neuronal responses. Delayed evoked-responses onset were also observed in layers 5/6, suggesting alterations in thalamocortical connectivity. Altogether, our data indicate that SCI immediately modifies local circuitries within the deprived cortex allowing supragranular layers to better integrate spontaneous corticocortical information, and thus modifying column excitability, and infragranular layers to better integrate evoked-sensory inputs to preserve subcortical outputs. These layer-specific neuronal changes may guide the long-term alterations in neuronal excitability and plasticity associated to the rearrangements of somatosensory networks and the appearance of central sensory pathologies usually associated with spinal cord injury.
A comprehensive computational model of animal biosonar signal processing
Authors: Ming Chen , Haro Stephanie , Simmons Andrea Megela , Simmons James A. 
Published: December 2020

AbstractComputational models of animal biosonar seek to identify critical aspects of echo processing responsible for the superior, real-time performance of echolocating bats and dolphins in target tracking and clutter rejection. The Spectrogram Correlation and Transformation (SCAT) model replicates aspects of biosonar imaging in both species by processing wideband biosonar sounds and echoes with auditory mechanisms identified from experiments with bats. The model acquires broadband biosonar broadcasts and echoes, represents them as time-frequency spectrograms using parallel bandpass filters, translates the filtered signals into ten parallel amplitude threshold levels, and then operates on the resulting time-of-occurrence values at each frequency to estimate overall echo range delay. It uses the structure of the echo spectrum by depicting it as a series of local frequency nulls arranged regularly along the frequency axis of the spectrograms after dechirping them relative to the broadcast. Computations take place entirely on the timing of threshold-crossing events for each echo relative to threshold-events for the broadcast. Threshold-crossing times take into account amplitude-latency trading, a physiological feature absent from conventional digital signal processing. Amplitude-latency trading transposes the profile of amplitudes across frequencies into a profile of time-registrations across frequencies. Target shape is extracted from the spacing of the object’s individual acoustic reflecting points, or glints, using the mutual interference pattern of peaks and nulls in the echo spectrum. These are merged with the overall range-delay estimate to produce a delay-based reconstruction of the object’s distance as well as its glints. Clutter echoes indiscriminately activate multiple parts in the null-detecting system, which then produces the equivalent glint-delay spacings in images, thus blurring the overall echo-delay estimates by adding spurious glint delays to the image. Blurring acts as an anticorrelation process that rejects clutter intrusion into perceptions.Author summaryBats and dolphins use their biological sonar as a versatile, high-resolution perceptual system that performs at levels desirable in man-made sonar or radar systems. To capture the superior real-time capabilities of biosonar so they can be imported into the design of new man-made systems, we developed a computer model of the sonar receiver used by echolocating bats and dolphins. Our intention was to discover the processing methods responsible for the animals’ ability to find and identify targets, guide locomotion, and prevent classic types of sonar or radar interference that hamper performance of man-made systems in complex, rapidly-changing surroundings. We have identified several features of the ears, hearing, time-frequency representation, and auditory processing that are critical for organizing echo-processing methods and display manifested in the animals’ perceptions.
Determination of digital technologies preferences of educational researchers
Authors: Fırat Mehmet , Altınpulluk Hakan , Kılınç Hakan 
Published: December 2020

PurposeThis study aims to investigate the preferences of 96 educational researchers on the use of digital technologies in scientific research.Design/methodology/approachThe study was designed as a quantitative-dominant sequential explanatory mixed-method research.FindingsDespite the spreading use of advanced technologies of big data and data mining, the most preferred digital technologies were found to be data analysis programs, databases and questionnaires. The primary reasons of using digital technology in scientific research were to collect data easily and quickly, to reduce research costs and to reach a higher number of participants.Originality/valueThe use of digital technologies in scientific research is considered a revolutionary action, which creates innovative opportunities. Through digitalized life, probably for the first time in history, the educational researchers have analytical information, which we can benefit from more than the individual's own statements in research involving human factor. However, there are a few studies that investigated the preferences of educational researchers who use digital technologies in their scientific research.
Development of a supply chain risk index for manufacturing supply chains
Authors: Alora Aswin , Barua Mukesh K. 
Published: December 2020

PurposeThe purpose of this paper is to identify, classify and prioritize supply chain risks faced by Indian micro small and medium manufacturing companies and to develop a comprehensive supply chain risk index.Design/methodology/approachPrimary data has been collected from 354 Indian micro small and medium enterprises on the different supply chain risks faced by them. An extensive literature review followed by expert's interview has been carried out in order to finalize the supply chain risks. A hybrid methodology consists of AHP and Fuzzy TOPSIS is applied for the data analysis. A sensitivity analysis has been done to check the robustness and consistency of the results.FindingsResults depict the importance of supply side and financial side risks faced by manufacturing supply chains, thus adding to the ongoing academic debate on the importance of supply chain finance solutions.Research limitations/implicationsStudy is limited to the scope of an emerging market. Generalization of results needs more systematic studies around the world in different supply chains.Practical implicationsSupply chain managers can consider the benchmark framed in this study in order to identify the health of their supply chain and to efficiently employ supply chain risk management strategies.Originality/valueThe current study is novel in developing a supply chain risk index using a hybrid AHP-Fuzzy TOPSIS methodology with a comprehensive list of 26 supply chain risks under 5 categories for an MSME supply chain. To the best of the authors’ knowledge, this is the first study incorporating financial risks in the development of a supply chain risk index.
Antecedents and consequences of stress in retailing: environmental expectations and promoter scoring
Authors: Lucia-Palacios Laura , Pérez-López Raúl , Polo-Redondo Yolanda 
Published: December 2020

PurposeThe aim of this paper is to examine the effects of the disconfirmation of expectations of crowding and mall accessibility, on stress and two marketing outcomes, satisfaction and promoter scoring.Design/methodology/approachData were obtained through two face-to-face surveys from mall shoppers that answered them at two different moments of their shopping experience, before entering the mall and before leaving it. Results are obtained from 230 customers that answered the two questionnaires.FindingsThe findings suggest that stress indirectly influences customer promoter scoring through satisfaction, while disconfirmation of expectations influences it directly and indirectly.Practical implicationsThese results also suggest that stress and disconfirmation of expectations about crowding and accessibility are important in determining promoter scoring. To reduce stress and increase satisfaction and promoter scoring, managers should focus on exceeding customers' expectations about mall accessibility and on ensuring that customers experience a lower level of crowding than they expected.Originality/valueThe article examines Net Promoter Scoring, an outcome that has attracted managers' attention but little is known about its antecedents. The paper provides evidence of the effect of disconfirmation of expectations and negative emotions on promoter scoring.
Brand outcomes in sales channel relationships: a guanxi theory perspective
Authors: Rong Weidong , Arnold Mark J. , Carlson Brad D. 
Published: December 2020

PurposeGuanxi is the foundation of business success in Chinese and other Eastern cultures, but little is known about the extent to which guanxi influences brand outcomes in channel relationships. The purpose of this study is to propose a novel theoretical framework of interpersonal and interorganizational guanxi relationships in a sales channel context and how these relationships influence brand outcomes.Design/methodology/approachThe authors conceptualize, develop and validate new scales measuring interpersonal and interorganizational guanxi and test the proposed model with a field study to validate the effects of guanxi on brand loyalty. The causal relationships between interpersonal guanxi and brand attitudes and affect are confirmed in a separate field experiment.FindingsFindings show that interpersonal guanxi is antecedent to interorganizational guanxi, and these two constructs have significant and differential direct and indirect effects on brand attitudes, brand affect and brand loyalty.Originality/valueNew multidimensional scales measuring both interpersonal and interorganizational guanxi were developed. Both interpersonal and interorganizational guanxi are conceptualized as second-order reflective constructs measured by the reflective first-order guanxi elements of personal and firm trust, personal and firm long-term orientation, xinyong (integrity), reciprocity, interaction and face.