bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fecal Carriage and Molecular Epidemiology of mcr-1-Harboring Escherichia Coli from Children in Southern China.
Authors: Mai Jialiang , Xiong Zhile , Yao Shuwen , Chen Xiangtang , Liang Bingshao , Gao Fei , Long Yan , Huang Zhouhong , Ai Xiaolan , Liang Zhuwei , Gao Xiurong , Gong Sitang , zhou Zhenwen 
Published: June 2020

Abstract

Objective: The increase of multidrug resistant Enterobacteriaceae bacteria has led to reintroduction of colistin for clinical treatments, and colistin has become a last resort for infections caused by multidrug resistant bacteria. Enterobacteriaceae bacteria carrying the mcr-1 gene are majorly related to colistin resistance, which may be the main reason for continued increase in the colistin resistance rate of Enterobacteriaceae. The purpose of this study was to investigate the sequence type and prevalence of bacteria harboring mcr-1 gene in the gut flora of children in Southern China. Method: Fecal samples (n=2632) of children from 3 medical centers in Guangzhou were cultured for Escherichia coli (E. coli). The mcr-1-harboring isolates were screened by Polymerase chain reaction (PCR). The colistin resistance transfer frequency was studied by conjugation experiments. DNA sequencing of seven housekeeping genes were used for multi locus sequence typing analysis (MLST).Result: PCR indicated that 21 isolates from the 2632 E. coli (0.80%) were positive for mcr-1; these strains were resistant to colistin. Conjugation experiment indicated that 18 of the mcr-1-harboring isolates could transfer colistin resistance phenotypes to E. coli J53. MLST analysis revealed that the 21 isolates were divided into 18 sequence types (STs); ST69 was the most common (14.3%), followed by ST58 (9.5%). Conclusion: These results demonstrate the colonisation dynamics and molecular epidemiology of mcr-1-harboring E. coli in the gut flora of children in Southern China, and the mcr-1 gene can be horizontally transmitted within species, it is necessary to monitor the mcr-1-harboring bacteria in children.
ABO Blood Type Has No Impact on Survival in Patients with Endometrial Carcinoma – A cohort study of 1074 patients
Authors: Li Yibing , Wu Qijun , Huo Jianing , He Junjian , Ma Haining , Ma Xiaoxin 
Published: June 2020

Abstract
Background: Endometrial carcinoma is one of the three major malignant tumors in gynecology. ABO blood type is associated with the prognosis of a variety of malignancies. This study assessed the relationship between ABO blood type and prognosis of endometrial carcinoma.Methods: We retrospectively analyzed the relationship between ABO blood type and endometrial carcinoma prognosis in patients with primary endometrial carcinoma who underwent surgery from Shengjing Hospital affiliated to China Medical University from January 2012 to February 2017. Univariate analysis, multivariate analysis, and stratified analysis were performed.Results: In the multivariate analysis, 2009 FIGO stage (HR=2.806, 95% CI=1.289, 6.109), Pathological tissue type (HR=0.199, 95% CI=0.079, 0.503) was an independent and important risk factor for OS. We divided the ABO blood type into A and non-A groups, B and non-B groups, O and non-O groups, AB and non-AB groups, and failed to measure the significant results of OS. After we excluded 9 patients who had recurrence, metastasis, or death within 1 year of enrollment, the OS-significant results were similar to those described above.Conclusions: Our study suggest that there is no association between ABO blood type and EC prognosis, and additional cohort studies are needed for validation.
Caregiver Burden and Coping Strategies in Caregivers of Elderly Patients with Stroke
Authors: Kazemi Azar , Azimian Jalil , Mafi Maryam , Allen Kelly-Ann , Motalebi Seyedeh Ameneh 
Published: June 2020

Abstract
Background

Coping strategies play a key role in modulating the caregiving burden. The present study was aimed to determine the severity of the burden of care and its relationship with coping strategies among caregivers of post-stroke elderly patients in Zanjan City, Iran.
Methods

A total of 110 caregivers of elderly patients who had previously had a stroke participated in this descriptive and cross-sectional study. A demographic checklist, Zarit Burden Interview, and Lazarus and Folkman questionnaires were used for data collection. Questionnaires were completed by the caregivers, who were selected using convenience sampling. The collected data were analyzed using Pearson's correlations and independent t-tests.
Results

The mean age of 110 caregivers participated in the study was 32.09 ± 8.70 years. The most commonly used coping strategies were reappraisal and seeking social support. Results of the independent t-test showed that male caregivers used the reappraisal strategy (t(110) = 2.76; p = 0.007) and responsibility (t(110) = 2.26; p = 0.026) significantly more than female caregivers. Pearson’s correlation showed a significant positive correlation between caregiver burden and emotional-focused strategies including escaping (r = 0.245, P = 0.010) and distancing (r = 0.204, P = 0.032).
Conclusions

Based on the results, caregivers with higher burden care used more negative coping strategies, such as escape and distancing. In order to encourage caregivers to utilize effective coping skills, appropriate programs should be designed and implemented to support caregivers. Use of effective coping skills to reduce the level of personal burden can improve caregiver physical health and psychological well-being.
CD73 positive adipose derived mesenchymal stem cells enhance cardiac repair with experimental myocardial infarction by promoting angiogenesis
Authors: Li Qiong , Hou Huifang , Li Meng , Yu Xia , Zuo Hongbo , Gao Jianhui , Zhang Min , Guo Zhikun 
Published: June 2020

Abstract
Background: Cardiovascular disease is the leading cause of death in developed and developing countries. The lack of effective regenerative therapies in the treatment of ischemia‐related diseases requires new therapies to improve clinical outcomes. Thus, MSCs have become a focus in stem cell treatment of myocardial injury. At present, most studies use mixed MSCs in vivo and in vitro. A promising therapeutic strategy for myocardial injury should be using the dominant subgroup with essential biological characteristics. The aim of this study was to utilize the dominant CD73 + subgroup of adipose derived mesenchymal stem cells (ADMSCs) for the therapy of myocardial infarction (MI). Methods: Adult mix gender SD rats, with a body weight of 230±18g, were randomly divided into sham operation group (SHAM), MI group (MI), mixed ADMSCs transplantation group (MI+ADMSCs), CD73 + ADMSCs transplantation group (MI+CD73 + ADMSCs) and CD73 - ADMSCs transplantation group (MI+CD73 - ADMSCs). CD73 + ADMSCs were isolated using flow cytometry and then cultured. Overexpression and inhibition of CD73 gene of ADMSCs using lentiviral vectors. Differential genes analysis of CD73 + ADMSCs vs. CD73 - ADMSCs were based on GO analysis. The effect of CD73 on the secretion of cytokines was measured by ELISA. Myocardial infarction model and cell transplantation model were replicated. Detection of cardiac function of rats by color doppler ultrasound after operation. The expression of VEGF and factor VIII and neovascularization were detected by immunohistochemistry and Western Blotting. Results: We demonstrated that, compared to mixed ADMSCs and CD73 - ADMSCs, CD73 + ADMSCs were more effective in the promotion function of cardiac recovery in a rat model of MI. CD73 + subset promoted vascular regeneration in myocardial injured regions. We also showed that expression of CD73 promoted secretion of VEGF, HIF-1α and HGF factors in ADMSCs. CD73 + ADMSCs displayed significantly different transcription profile compared to CD73 - ADMSCs, in particular, concerning VEGF pathways. Conclusions: Overall, CD73 + ADMSCs were the dominant subgroup and the presence of the surface marker CD73 can be used as a MSCs cell quality control for treatment of myocardial injury by angiogenesis.
Nature and outcome of malignant goiter: a single institutional experience from the eastern province of Saudi Arabia
Authors: Daoud Mohamed Yasser Ibrahim , Zakaria Ossama M , Suwaigh Abdul Mohsen Al 
Published: June 2020

Abstract
Background: Recent years have witnessed a dramatic increase in the number of malignant goiters among adolescents and adults.Aim: The aim of this work is to review patients with goiter, as regards, their nature, demographic characterization, clinical presentation and preoperative histopathological data obtained by Fine Needle Aspiration Cytology (FNAC). It also aimed to review; the operative data and post-operative histopathological pattern of all patients from a tertiary care hospital in the Eastern Province of Saudi Arabia. Moreover, the current retrospective study was designed to evaluate the nature, line of management and outcomes of malignant goiter in the same geographical region.Patient and Methods: The study took place in a period of 4 years from December 2015 to January 2019. Patients demographic data, clinical presentation, Intra-operative findings. Pre and postoperative histopathological staging and grading were all recorded. postoperative follow up whether early or late were also included.Results: A total of 65 patients underwent surgery. The female to male ratio was found to be 5:1 (48 females and 17 males). Patients’ age ranged from 8 to 70 year with the mean of 34.4 ±11.7 (mean ± SD). Solitary nodule was the main presentation in 80% of cases while 20% were multinodular swellings. Symptoms of increased thyroid hormone functions were recorded in 13 patients (20%). Papillary carcinoma was recorded in 52 patients (80%) while 10 patients were having follicular carcinoma. The remaining three patients (4.6%) suffered of lymphoma, no medullary or anaplastic tumors were recorded.Conclusion: Thyroid cancer is quiet high among Saudi patients within the Eastern Province, the reason needs to be thoroughly investigated. Papillary type of carcinoma is the most commonly encountered pathological type of malignancy followed by follicular carcinoma and lymphoma. Further prospective and meta analytic studies may be needed for a more accurate and thorough assessment of the reasons that led to the increasing number of thyroid malignancies and their correlation to environmental factors.
Relationship of Lifestyle Habits to Health-Related Quality of Life of Recently Diagnosed Breast Cancer Between Younger and Older Women in China
Authors: Zheng Chao , Yu Li-Xiang , Jia Hong-ying , Cui Shu-De , Tian Fu-Guo , Fan Zhi-Min , Geng Cui-Zhi , Cao Xu-Chen , Yang Zhen-Lin , Wang Xiang , Liang Hong , Wang Shu , Jiang Hong-Chuan , Duan Xue-Ning , Wang Hai-Bo , Li Guo-Lou , Wang Qi-Tang , Zhang Jian-Guo , Jin Feng , Tang Jin-Hai , Li Liang , Zhu Shi-Guang , Zuo Wen-Shu , Wang Fei , Zhou Fei , Xiang Yu-Juan , Guo Ming-ming , Wang Yong-Jiu , Huang Shu-ya , Liu Liyuan , Yu Zhi-Gang 
Published: June 2020

Abstract
Background: Breast cancer is the most common cancer among women in China. Hence, how best to live with and improve the health-related quality of life (HRQoL) of this growing population of women is thus becoming of great public health importance. The aim of the study was to evaluate the relationship of lifestyle habits to HRQoL among younger and older women who were initially diagnosed with breast cancer within the first two weeks, determine the contribution of lifestyle habits factors on HRQoL.Methods: A multi-center, hospital-based, case control study was conducted among breast cancer women from 22 hospitals in 11 provinces or municipalities in northern and eastern China from April 2012 to April 2013. The Functional Assessment of Cancer Therapy-Breast Cancer (FACT-B) simplified Chinese version 4 was used to measure HRQoL. Chi-square test, ANOVA and Multivariable generalized linear models were conducted to verify differences in HRQoL between two groups and to evaluate the contribution of lifestyle habits factors ( including smoking, passive smoking, alcohol intake, Tea, Coffee, Sleep satisfaction, Current life satisfaction, Physical activity, BMI) on HRQoL of breast cancer patients.Results: 1199 eligible breast cancer patients were used for analysis. Younger women (age < 50 years) appeared to show lower scores than older women (age ≥ 50 years) in HRQoL subscales including emotional well being (p = 0.003), functional well being (p = 0.006), breast cancer subscale (p = 0.038), and FACT-B Total scores (p = 0.028). Tea and alcohol consumption, very satisfied with sleep and current life were the strongest predictors of higher HRQoL in younger group. Meanwhile, no coffee consumption, frequent participation in physical activities, high sleep satisfaction and current life satisfaction were the key predictors of higher HRQoL in older breast cancer women.Conclusion: The relationship of the nine lifestyle habit items to HRQoL were different between younger and older women. Associated variable of low HRQoL can help clinicians take intervention early in order to improve the prognosis of breast cancer patients.
Utilizing network pharmacology to explore the underlying mechanism of Cinnamomi Ramulus in Zika Fever induced by Zika Virus
Authors: Zhang Qiong , Lin Xuemei , Lai Yanni , Xu Jiangtao , Chen Yuting , Wang Xiao , Liu Xiaohong , geng Li 
Published: June 2020

Abstract
Background: Zika fever is a mosquito-borne infection induced by Zika virus (ZIKV),effective drug and vaccine against ZIKV infection is still unavailable. Cinnamomi ramulus (CR) is a traditional Chinese herb with a long history ,can stimulate secretion of sweat and relieve exterior syndrome. The aim of this research was to evaluate the effect of CR against ZIKV and uncover its mechanism of action by network pharmacology.Methods: Cell viability assay, qRT-PCR assay and western blot assays were perfermed to evaluate anti-ZIKV activity in vitro. Survival rate, body weight were observed and viremia was detected in AG6 mice. Drug- target-disease networks, GO enrichment, and KEGG pathway analysis was established to clarify the therapeutic mechanismsits. Results: In this study, we find that CR can alleviate CPE after Zika virus infection. In the premixed administration mode, CR showed superior activity to inhibit viral RNA replication and protein expression in cells. Orally administered CR effectively protected AG6 mice infected with lethal doses of ZIKV, conferring 50% or 20% survival rate at a dosage of 900mg/ml or 450mg/ml, reducing body weight loss, inhibiting viral RNA replication. Beta-sitosterol, DBP, α-Longipinen, (-)-alpha-cedrene, ()-alpha-Longipinene may the main active compound and PTGS2, GABRA1, PTGS1, PTGES and CCR5 may the main targets. 176 biological processes, 16 cell components, 27 molecular functions, and 37 pathways were significantly identified in GO enrichment and pathway analysis. Conclusions: These results reveal that CR treatment of Zika fever is mainly related to PTGS1 and PTGS2 regulated prostaglandin release, metabolism, and inflammation response.
Prevalence of Cardiovascular Disease Risk Factors in an Indigenous Vedda Community in Sri Lanka: Do These Communities Need a Targeted Health Promotion Strategy?
Authors: Dalpatadu Kaluthanthri Patabndi Chamila , Katulanda Prasad , Katulanda Gaya , Herath Priyanka , Wanninayake Leonard , Niroshan Chrishantha , Jayasinghe Saroj 
Published: June 2020

Abstract
Background: The indigenous population in Sri Lanka called “Veddas” estimated to be around 0.05% of the total population in the island. This population is unique that they used to live mostly in jungles with primitive lifestyles. The objective was to study the cardiovascular risk factors in this indigenous (Vadda) community.Methods: A descriptive cross-sectional study was carried out with convenience sampling method, by inviting all adults (> 18 years) in the indigenous village Pollebedda. Height, weight and blood pressure was measured, and fasting blood samples were analyzed for glucose, lipids and haemoglobin. Data were analyzed using SPSS (version 22)0.Results: Response rate was approximately 39%. Out of total 121 participants 47 were males and 74 were females. The mean age was 44.29 +14.75 years and 12 were elders> 65 years of age. In the sample 17.3% were obese (BMI>27.5) while 24.8%% were overweight (BMI 23-27.5). The crude prevalence of diabetes, hypertension and raised total cholesterol were 7.2%, 12.4% 7.7% respectively. Prevalence of low HDLC (< 40mg/dL in men,< 50 mg/dL in women), high LDLC (>160 mg/dL) and elevated triglycerides (>150mg/dL) were 75.2%, 7.7% and 25.6% respectively. Isolated hypertriglyceridemia was present in 20.5%.Conclusions: There is a rising trend of obesity and diabetes in this indigenous community compared to previous study. This highlights that the indigenous peoples in Sri Lanka share the relatively high rates of coronary artery risk factors observed among other indigenous groups around the world. Changes in lifestyle and diet, poverty, poor health seeking behavior and limited access to health care due to limitation in transport are possible risk factors for this trend which needs to be further evaluated. A local and global initiative is required to control cardiovascular disease risk factors among indigenous groups.
Manifestations in Neonates Born to COVID-19 Positive Mothers: a Case Series
Authors: Jain Parul , Thakur Anup , Kler Neelam , Garg Pankaj 
Published: June 2020

Abstract
Coronavirus disease (COVID-19) or severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), a novel viral infection is currently a global pandemic. Primarily transmitted by aerosols and fomites, limited information exists on its vertical transmission from mother and clinical outcomes in neonates. We report two neonates born to SARS-CoV-2 positive women.
Saudi Vision 2030 Dynamic Input-Output Table: A Tool for Quantifying the Sustainable Development Targets of Saudi Arabia
Authors: Soytas Mehmet Ali , Havrlant David 
Published: June 2020

Abstract
This paper introduces an approach that combines macroeconomic forecasts with the input-output analysis methods to produce long-term projections of input-output tables (IOTs), with an emphasis on key targets of Saudi Vision 2030, Saudi Arabia’s blueprint for economic diversification and sustainable growth. A significant advantage of the input-output framework is its high sectoral granularity, allowing it to capture the impacts of adjustments to final demand or government policies with respect to individual sectors. Our hybrid approach enables the introduction of different sectoral growth paths, so that Vision 2030’s transformation plan is reflected appropriately in the projected IOTs. The framework is flexible enough to accommodate sudden adjustments with relative ease, such as the introduction of new technologies or entire sectors into the economy. Saudi Vison 2030 includes a set of targets relating to economic diversification into non-oil sectors, improved energy efficiency, the introduction of new technologies, social transformation, and the support of selected emerging sectors. The ultimate aim of the vision is to create an acceleration towards sustainable growth and development in the Saudi economy. Since these policies are expected to have a substantial impact on the economy, quantifying the economic implications of diversification and sectoral shifts require an adequate and flexible tool for projecting and evaluating structural adjustments for a better decision making.
Mitochondria-Related Core Genes in Breast Cancer: Potential Diagnostic, Prognostic Markers and Therapeutic Targets
Authors: Yan Li-rong , Wang Ang , Lv Zhi , Yuan Yuan , Xu Qian 
Published: June 2020

Abstract
Background: Mitochondria-nuclear cross talk and mitochondrial retrograde regulation are involved in the genesis and development of breast cancer (BC). Therefore, mitochondria can be regarded as a promising target for BC therapeutic strategies. In the present study, we aimed to seek the potential biomarkers of BC diagnosis and prognosis and also the molecular therapeutic targets from the perspective of mitochondrial dysfunction.Methods: The microarray data of mitochondria-related encoding genes of BC were downloaded from GEO including GSE128610 and GSE72319. GSE128610 was treated as test set and validation sets consisted of GSE72319 and TCGA, which were used for identifying mitochondria-related differential expressed genes (mrDEGs). GO analysis and KEGG mapping of validated mrDEGs was performed by STRING and KEGG mapper, respectively. PPI network was constructed by STRING. MCODE was applied to modeling prediction of PPI and hub mrDEGs were screened by cytohubba. The online tool of Kaplan Meier plotter was adopted to analyze the correlation between hub mrDEGs and the overall survival of BC patients.Results: A total of 23 up-regulated and 71 down-regulated mrDEGs were identified and validated. Enrichment analyses indicated that mrDEGs were associated with several biological processes, including cell migration, cell surface receptor signaling pathway, cell differentiation and cell communication. Meanwhile, these genes were mapped in PI3K-ALT pathway, TGF-beta pathway, evading apoptosis pathway and resistance to chemotherapy pathway. Moreover, 9 hub mrDEGs were identified. Among them, up-regulated hub mrDEGs contained FN1, BGN, EFNA3, COL5A2 and SEMA3F, and down-regulated hub mrDEGs comprised RHOQ, SEMA3A, NRP1 and DDR2. Overall survival analysis suggested that the up-regulated FN1 and SEMA3F and down-regulated DDR2 conferred to poor BC prognosis.Conclusion: The 9 hub mrDEGs, especially FN1, SEMA3F and DDR2, were likely to regulate mitochondrial function and might be novel biomarkers of BC diagnosis and prognosis as well as the therapeutic targets.
Analysis of functional indices indicates multiple organ function damage in patients with severe Coronavirus Disease 2019 (COVID-19)&nbsp;pneumonia
Authors: Feng Shiyan , Wang Fengxin , Cai Zhao , Wu Weibo , Li Yinfeng , Chen Li , Chen Chuming , Cao Mengli , Peng Ling , Jiang Xiao , Zhang Peiyan , Li Jianming , Yang Liuqing , Liu Yingxia , Liu Lei , Yang Liang , Wang Fuxiang 
Published: June 2020

Abstract
Background: This study aims to analyze the changes and significance of organ function indices in patients with severe Coronavirus Disease 2019 (COVID-19) pneumonia for prediction of major organ damages and guiding treatment schemes.Methods: 63 patients with severe COVID-19 pneumonia were selected as the severe group and 73 patients with mild syndromes were selected as the mild group. SAS9.4 software was used for statistical analysis of the data.Results: Levels of ALT, AST, cTnI, Cr, PT, APTT and D-DIC of the severe group were significantly higher while PLT was lower than those of the mild group. The data of all quantitative variables were converted into categorical variables. Significantly higher levels of AST, ALB, D-DIC and higher proportion of bilateral lung involvement were observed from the severe group comparing to those in the mild group, while the difference in the other indices between the two groups was insignificant in statistical perspective.Discussion: There are significant differences in the levels of multiple organ function indices between the severe group and the mild group of patients with COVID-19 pneumonia infection. Through examining the relevant indices, conditions of patients’ multiple organ function damage could be predicted and used as guidance of treatment.