bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis Strategy for Assisted Selection of Rice Eating Quality by Using Starch Properties
Authors: Li Xu , Mao Ting , Wang Yu , Sui Xin , Ren Hai , Lü Hong Xiao , Ma Chang , Du Meng , Fu Dong Li 
Published: July 2020

Abstract
Background

Eating quality is the main factor affecting the commodity value of commercial rice. However, its determination relies on sensory evaluations, which require large samples, are easily influenced by subjective factors and are time consuming. In order to make the determination of eating quality more efficient, a set of recombinant inbred lines (RILs) obtained from the hybridization of indica and japonica rice was used as the test material to analyze the relationship between starch characteristics and the eating quality (EQ) score, from sensory evaluation. An implementation protocol was proposed for starch assisted-selection of eating quality.
Results

Among the RILs, the values of the measured parameters for amylose content (AC), trough viscosity (TV), final viscosity (FV), breakdown (BD) and setback (SB) were significantly correlated with the EQ values ​​obtained by sensory evaluation. The RILs with higher EQ scores, generally had low AC, TV, FV and SB, but high BD. By normalizing and summing the AC, TV, FV, SB and BD indicators, a comprehensive indicator—viz., the starch properties quality (SPQ)—was established, to assess starch quality. The SPQ value was better correlated with EQ than any of the individually measured parameters, suggesting that the model is valid. This method requires much smaller rice samples than sensory evaluations, i.e., three grams of milled rice flour.
Conclusions

Therefore, the SPQ indicator is a rapid, objective and convenient technique for the rapid assessment of rice eating quality in a large number of crossbreeding progeny, especially early-generation progeny, from which only small samples of rice grains are available.
Enhanced recovery after surgery (ERAS) protocol in Oblique lumbar interbody fusion(OLIF): a case-controlled study
Authors: Li Renjie , Shao Xiaofeng , Jiang Weimin 
Published: July 2020

Abstract
Background Enhanced recovery after surgery (ERAS) attempts to decrease the surgical stress response to minimize postoperative complications and improve functional rehabilitation after major surgery, but it have not been widely utilized in spinal surgery. This study is to evaluate the implementation of an ERAS pathway for patients undergoing oblique lumbar interbody fusion (OLIF) surgery.Methods This was a retrospective cohort study of patient who underwent OLIF in 2018 prior to ERAS (“pre-ERAS” ,n=23) and in 2019 after ERAS was instituted (“ERAS”, n=24). Major outcomes were collected included demographics, length of hospital stay, financial cost, postoperative complications, off-bed time and perioperative factors. Visual Analogue Scale (VAS) was used to evaluate the pain. The ERAS pathway and compliance with pathway elements were also recorded.Results After ERAS implementation, we found no significant differences in the baseline characteristics between the two groups. In our study, the mean stay in the hospital was significantly lower (p= 0.033) in the ERAS group (15.3±3.9 days) compared to the standard pathway group (13.0±3.1 days). In comparison to the standard group, we also found a variation between the financial costs of surgery and hospitalization [(16446.5±4353.3)vs(14237.7±2784.9) USD, P < 0.05]. The ERAS group manifested a lower blood loss compared with the pre-ERAS group with statistical significance [(68.3±57.1)vs(119.3±104.8) ml, P < 0.05]. There was no significant difference in operative time, complications, and 30-d readmission rates ( P > 0.05 ). Pain scores between the two groups showed a significant difference during the 3th hour and 6th hour (P < 0.05).Conclusion Institution of an ERAS protocol appears to accelerate functional recovery and reduce length of stay, financial costs and decreased pain.
Presymptomatic viral shedding and infective ability of Severe Acute Respiratory Syndrome coronavirus 2
Authors: Nissen Karolina , Hagbom Marie , Krambrich Janina , Akaberi Dario , Sharma Sumit , Ling Jiaxin , Hoffman Tove , Bondeson Kåre , Svensson Lennart , Lundkvist Åke , Salaneck Erik 
Published: July 2020

Abstract
We report repeated rRT-PCR detection and infective ability of Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2) from a health care worker three days prior to mild COVID-19 symptoms. We propose that this provides evidence for potential early presymptomatic transmission of SARS-CoV-2 and that contagiousness may arise shortly after exposure.
Rate and Neonatal Outcomes Among Instrument Assisted Vaginal Birth in 12 Public Hospitals in Nepal
Authors: Thakur Nishant , Sunny Avinash K , Gurung Rejina , Basnet Omkar , Litorp Helena , KC Ashish 
Published: July 2020

Abstract
Background Instrument assisted vaginal birth (IVB) is an effective intervention for deliveries complicated by prolonged labour or fetal distress, but its use is declining in many low-resource settings. In this paper, we examined intra-hospital rates of IVB, factors associated, and neonatal outcomes after IVB in Nepal. Methods This is a prospective cohort study of all deliveries conducted in 12 public hospitals (4 high volume, 4 medium volume and 4 low volume) across Nepal for 18 months . We calculated the rate of IVB and used logistic regression to assess the association between IVB and neonatal morbidity (Apgar score < 7 at 5 minutes, shoulder dystocia) and mortality.Results A total of 81,581 deliveries were included in the study, of which 3001 (3.4%) were IVBs., while rates in high volume, medium volume, and small volume hospitals were 3.6%, 3.7% and 1.2% respectively. The odds of Apgar score < 7 at 5 minutes was almost three-fold (aOR 2.92, 95% CI, 2.49-3.42) with IVB compared to spontaneous vaginal birth (SVB). The odds of shoulder dystocia was three-fold (aOR 3.04, 95% CI, 2.19-4.22) with IVB compared to SVB. The odds of first day mortality was lower in medium volume (aOR-0.57, 95% CI, 0.42-0.78) hospitals compared to high volume hospitals. Conclusions The rate of IVB varied by volume of hospital. The neonatal outcome were poor among the babies born to IVB, and neonatal outcomes were worse after IVB at high-volume hospitals. Further studies to explore factors determining the rate of IVB and better neonatal outcomes.
Pregnancy vs. Paycheck: A qualitative study of patient’s experience with employment during pregnancy at high risk for preterm birth
Authors: Wheeler Sarahn M. , Massengale Kelley E. C. , Adewumi Konyin , Fitzgerald Thelma A. , Dombeck Carrie B. , Swezey Teresa , Swamy Geeta K. , Corneli Amy 
Published: July 2020

Abstract
Background: Pregnant women with a history of preterm birth are at risk for recurrence, often requiring frequent prenatal visits for close monitoring and/or preventive therapies. Employment demands can limit uptake and adherence to recommended monitoring and preterm birth prevention therapies. Method: We conducted a qualitative descriptive study using in-depth interviews (IDIs) of pregnant women with a history of preterm birth. IDIs were conducted by trained qualitative interviewers following a semi-structured interview guide focused on uncovering barriers and facilitators to initiation of prenatal care, including relevant employment experiences, and soliciting potential interventions to improve prompt prenatal care initiation. The IDIs were analyzed via applied thematic analysis.Results: Participants described multiple ways that being pregnant impacted their earning potential, ranging from voluntary work-hour reduction, involuntary duty hour reductions by employers, truncated promotions, and termination of employment. Participants also shared varying experiences with workplace accommodations to their work environment and job duties based on their pregnancy. Some of these accommodations were initiated by a collaborative employee/employer discussion, others were initiated by the employer’s perception of safe working conditions in pregnancy, and some accommodations were based on medical recommendations. Participants described supportive and unsupportive employer reactions to requests for accommodations.Conclusions: Our findings provide novel insights into women’s experiences balancing a pregnancy at increased risk for preterm birth with employment obligations. While many women reported positive experiences, the most striking insights came from women who described negative situations that ranged from challenging to potentially unlawful. Many of the findings suggest profound misunderstandings likely exist at the patient, employer and clinical provider level about the laws surrounding employment in pregnancy, safe employment responsibilities during pregnancy, and the range of creative accommodations that often allow for continued workplace productivity even during high risk pregnancy.
Clinical Efficacy of Percutaneous Endoscopic Lumbar Discectomy for the Treatment of Lumbar Spinal Stenosis in Elderly Patients
Authors: Li hua , Ou Yufu , Xie Furong , Liang Weiguo , Tian Gang , Li Hongyu 
Published: July 2020

Abstract
Background: Although: percutaneous endoscopic lumbar discectomy (PELD) is increasingly being used to treat lumbar degenerative disease, the treatment of elderly patients with lumbar spinal stenosis (LSS) involves considerable uncertainty. The purpose of this study was to evaluate the safety and effectiveness of PELD for the treatment of LSSin elderly patients aged 65 years or older. Methods: In this retrospective review, 136 patients aged 65 years or older who underwent PELD to treat LSS were evaluated. The patients were divided into two groups, group A (age 65-74) and group B (age ≥ 75), and perioperative data were analyzed. The Japanese Orthopaedic Association (JOA) score, visual analog scale (VAS) score, and MacNab classification were used to evaluate postoperative clinical efficacy. Results: All patients successfully underwent the operation with satisfactory treatment outcomes. Compared to preoperative scores, the self-reported scores or pain while performing daily activities were significantly improved in both treatment groups (P<0.05).No statistically significant between-group differences were observed in operation time, intraoperative blood loss, postoperative bed rest, and postoperative hospital stay (P > 0.05). The overall postoperative complication rate was similar between the two groups.Moreover, no statistically significant differences in VAS-back pain scores, VAS-leg pain scores, JOA scores, and MacNab classification were found between the groups at the 3-month and 1.5-year follow-up examinations (P>0.05).Conclusion: PELD is safe and effective for the treatment of LSS in elderly patients. Age is not a contraindication for decompressive lumbar spine surgery. PELD has advantages such as reduced trauma, fewer anesthesia-related complications, and a fast postoperative recovery.Elderly patients should be considered good candidates for lumbar decompression surgery using minimally invasive techniques.
Investigating the Frequency of Free-living Amoeba in Water Resources of Bandar Abbas City, Hormozgan Province, Iran 2019-2020
Authors: Attariani Homa , Turki Habibollah , Shoja Saeed , Salahi-Moghaddam Abdoreza , Ghanbarnejad Amin , Shamseddin Jebreil 
Published: July 2020

Abstract
Objective
These amoebas can cause dangerous illnesses when they accidentally enter the human body, so it is necessary to determine various forms of organisms in water resources to prevent the danger they can cause risks to human health. Currently, in Bandar Abbas, there is no sufficient information about the distribution of Acanthamoeba, and we intended to study its frequency.
Results
Out of 83 water samples collected from different resources in the city, 31 plates (37.3%) were found to be positive for free-living amoebae. Of these, five were identified (6%) by culture method and 8 (9.6%) by molecular method. Positive sample sequence analysis enables us to distinguish two genotypes of T4 and T15 in this study.
Ganglioneurofibroma Arising Within the Extralobar Pulmonary Sequestration
Authors: Liu Yuanyuan , Wu Wenbin , Gong Longbo , Zhang Miao 
Published: July 2020

Abstract
Background: Neurogenic tumor arising within the pulmonary sequestration (PS) is rare.Case Presentation: A 42-year-old asymptomatic female was referred to our hospital for work-up of extralobar PS. The independent feeding artery from the thoracic aorta was confirmed by three-dimensional computed tomography angiography (3D-CTA). Extrapulmonary lesions were excluded by whole-body radiographical examinations. Uniportal thoracoscopic resection of the sequestrated lung and mediastinal lymph node sampling was performed successfully. Finally, ganglioneurofibroma within the PS was confirmed by pathological staining. The patient was discharged from the hospital on the 3rd postoperative day without tumor recurrence a year after surgery.Conclusion: Preoperative 3D-CTA is useful to verify the aberrant vessels of PS; whereas an elaborate diagnostic work-up after a timely resection is necessary for possible subsequent management and follow-up plan.
One-step heating strategy for efficient solubilization of recombinant spider silk protein from inclusion bodies
Authors: Cai Hui , Chen Gefei , Yu Hairui , Tang Ying , Xiong Sidong , Qi Xingmei 
Published: July 2020

Abstract
Background: Spider silk is a proteinaceous fiber with remarkable mechanical properties spun from spider silk proteins (spidroins). Engineering spidroins have been successfully produced in a variety of heterologous hosts and the most widely used expression system is Escherichia coli (E. coli). So far, recombinantly expressed spidroins often form insoluble inclusion bodies (IBs), which will often be dissolved under extremely harsh conditions in a traditional manner, e.g. either 8 mol/L urea or 6 mol/L guanidine hydrochloride, highly risking to poor recovery of bioactive proteins as well as unexpected precipitations during dialysis process. Results: Here, we present a mild solubilization strategy—one-step heating method to solubilize spidroins from IBs, with combining spidroins’ high thermal stability with low concentration of urea. A 430-aa recombinant protein (designated as NM) derived from the minor ampullate spidroin of Araneus ventricosus was expressed in E. coli, and the recombinant proteins were mainly present in insoluble fraction as IBs. The isolated IBs were solubilized parallelly by both traditional urea-denatured method and one-step heating method, respectively. The solubilization efficiency of NM IBs in Tris-HCl pH 8.0 containing 4 mol/L urea by one-step heating method was already comparable to that of 7 mol/L urea with using traditional urea-denatured method. The effects of buffer, pH and temperature conditions on NM IBs solubilization of one-step heating method were evaluated, respectively, based on which the recommended conditions are: heating temperature 70–90°C for 20 min, pH 7.0–10, urea concentration 2–4 mol/L in normal biological buffers. The recombinant NM generated via the one-step heating method held the potential functions with self-assembling into sphere nanoparticles with smooth morphology.Conclusions: The one-step heating method introduced here efficiently solubilizes IBs under relatively mild conditions compared to the traditional ones, which might be important for the downstream applications; however, this protocol should be pursued carefully in terms of urea-induced modification sensitive applications. Further, this method can be applied under broad buffer, pH and temperature conditions, conferring the potential to apply to other thermal stable proteins.
Improved Prognosis for Recurrent Epithelial Ovarian Cancer By early Diagnosis and 125I Seeds Implantation During Suboptimal Secondary Cytoreductive Surgery: A Case Report and Literature Review
Authors: xiao lin , Tang Junying , Li Wenbo , Xu Xuexun , Zhang Hao 
Published: July 2020

Abstract
Background: Epithelial ovarian cancer (EOC) has the worst prognosis in all of gynecologic malignant tumors because of its high recurrence and eventually chemo-resistance. Early diagnosis of recurrence is crucial to avoid diffuse dissemination. Traditional treatment failure in recurrent ovarian cancer remains a challenge for clinicians. On the other hand, 125I brachytherapy has been accepted as a useful treatment for multiple advanced cancers in recent years. However, its success in advanced epithelial ovarian cancer is limited. Here we report a case of recurrent ovarian cancer who had been early diagnosis of isolated recurrence and successfully treated with 125I seeds implantation during suboptimal cytoreductive surgery.Case presentation: A 59-year-old woman presented with recurrent epithelial ovarian cancer who have had a medical history of ovarian cancer stage IIIB and an R0 resection had been achieved before presented in our hospital. She underwent suboptimal secondary cytoreductive surgery after four cycles of chemotherapy with little effectiveness and severe chemotherapy-related side effects. Approximately 70% of the cancer-bulk was resected during surgery. For residual lesion which fixed around the right ureter and right external iliac vessel, 125I seeds implantation was performed. Postoperatively, the patient was treated with two cycles of combination chemotherapy with paclitaxel and carboplatin. The patient was free of disease at 26 months’ follow-up period. Conclusion: In recurrent EOC patients with unresectable isolated lesion, salvage 125I seeds implantation are feasible and may contribute to survival.
Genome sequencing and comparative genomic analysis of highly and weakly virulent strains of Sclerotium rolfsii, the causal agent of peanut stem rot
Authors: Yan Liying , Wang Zhihui , Song Wanduo , Fan Pengmin , Kang Yanping , Lei Yong , Wan Liyun , Huai Dongxin , Chen Yuning , Wang Xin , Sudini Hari , Liao Boshou 
Published: July 2020

Abstract
Background
Stem rot caused by Sclerotium rolfsii is a very important soil-borne disease to peanut worldwide. S. rolfsii is a necrotrophic plant pathogenic fungus with an extensive host range and worldwide distribution. It can infect peanut stems, roots, pegs and pods, leading to varied yield losses. S. rolfsii strains GP3 and ZY collected from peanut in different provinces of China exhibited significant difference in virulence on peanut by artificial inoculation test. In this study, de-novo genome sequencing of these two distinct strains was performed in the hopes of revealing the genomic basis of virulence differentiation.
Results
Scleotium rolfsii strains GP3 and ZY, with weak and high virulence on peanut, respectively, exhibited similar growth rate and oxalic acid production in laboratory. The genomes of S. rolfsii strains GP3 and ZY were sequenced by Pacbio long read technology and exhibited 70.61 Mb and 70.51 Mb, with contigs of 27 and 23, and encoded 17,097 and 16,743 putative protein genes, respectively. Comparative genomic analysis revealed that the pathogenicity-related gene repertoires, which might be associated with the virulence, diverged between GP3 and ZY. Detailed analysis further revealed 58 and 45 unique pathogen-host interaction (PHI) genes in GP3 and ZY, respectively. The ZY strain had more carbohydrate-active enzymes (CAZymes) in its secretome than GP3, especially in the glycoside hydrolase family (GH), the carbohydrate esterase family (CBM), and the polysaccharide lyase family (PL). GP3 and ZY also had different effector candidates. These results indicated that differences in PHI, secreted CAZymes, and effectors may play important roles in virulence differentiation between these two strains.
Conclusions
The data provided a further understanding of the S. rolfsii genome. Detailed comparative genomic analysis provided clues into the genomic basis of its necrotrophic lifestyle and virulence diversification.
The Kalmag Model as a Candidate for IGRF-13
Authors: Baerenzung Julien , Holschneider Matthias , Wicht Johannes , Lesur Vincent , Sanchez Sabrina 
Published: July 2020

Abstract
We present a new model of the Geomagnetic field spanning the last 20 years and called Kalmag. Deriving from the assimilation of CHAMP and SWARM vector field measurements, it separates the different contributions to the observable field through parameterized prior covariance matrices. To make the inverse problem numerically feasible it has been sequentialized in time though the combination of a Kalman filter and a smoothing algorithm. The model provides reliable estimates of past, present and future mean fields and associated uncertainties. The version presented here is an update of our IGRF candidates, the amount of assimilated data has been doubled and the considered time window has been extended from [2000.5,2019.74] to [2000.5,2020.33].
A precise navigation device for fixation of patella fractures with modified K-wire tension band: design and application
Authors: Wang Fuming , Xiong Haolan , Long Xiaotao , Li Yang , Chen Xiaohua , Wang Gang 
Published: July 2020

Abstract
Background Traditionally, the technique of modified tension band wires (MTBW) has been the most commonly used surgical procedure. The purpose of this study is to design a precise navigation device that can obtain a standard position of K-wires for (MTBW), and to compare the precise MTBW (P-MTBW) by a navigation device with the conventional MTBW (C-MTBW) by hands in a retrospective study.Methods The device is designed by solidworks2012 software (USA), which can provide a precise guidance for obtaining a parallel K-wires. In addition, it can set the distance between two k-wires and the level of k-wires below patellar anterior surface. From June 2014 to August 2018, a total of 112 patients are employed in this retrospective study. The patients are divided into P-MTBW group and C-MTBW group according to the surgical technique with or without the precise navigation device. We need to record and analyze the operation time and the number of fluoroscopy, postoperative internal fixation imaging, knee function and complications.Results There were 54 patients in P-MTBW group and 58 patients in C-MTBW group. There were statistically significant differences(P＜0.001) in the operation time between P-MTBW group (39.5±4.7; range, 32–49 minutes) and C-MTBW group (53.7±6.8; range, 42–71 minutes). The number of intraoperative fluoroscopy was significantly less (P＜0.001) in P-MTBW group(4.2±1.4) versus that of C-MTBW group (8.3±2.7). According to Iowa knee score, there was no significant difference (P=0.268) in function between the two groups. According to our own evaluation criteria for MTBW, anyone in the P-MTBW group was excellent and 26 patients were excellent, 20 patients were good and 2 patients were fair in the C-MTBW group.Conclusion The navigation device can reduce operation time and intraoperative fluoroscopy frequency. P-MTBW fixation is an accurate and effective surgical procedure for patella fractures.