bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cryogenic platform for coupling color centers in diamond membranes to a fiber-based microcavity
Authors: Salz M. , Herrmann Y. , Nadarajah A. , Stahl A. , Hettrich M. , Stacey A. , Prawer S. , Hunger D. , Schmidt-Kaler F. 
Published: July 2020

AbstractWe operate a fiber-based cavity with an inserted diamond membrane containing ensembles of silicon vacancy centers (SiV$${}^{-}$$

-

) at cryogenic temperatures $$\ge 4~$$

≥
4

K. The setup, sample fabrication and spectroscopic characterization are described, together with a demonstration of the cavity influence by the Purcell effect. This paves the way towards solid-state qubits coupled to optical interfaces as long-lived quantum memories.
History of the Pan African Paediatric Surgery Association (PAPSA)
Authors: Elhalaby E. A. , Rode H. , Lakhoo K. 
Published: July 2020

AbstractThe Pan African Paediatric Surgery Association (PAPSA) was formed in 1994. The need for an organisation in Africa to voice children’s surgery and the trials and tribulations in forming this organisation was covered in this journal 2 years ago (Heinz R, Kyambi J, Lakhoo K. Surg Int 34(5):499–504, 2018). This article covers the history of the organisation post inception in 1994 to date. The near disbanding of the organisation due to political unrest and wars in Africa, to its success in the recent decade is highlighted in this manuscript.
Intermittent versus continuous enteral nutrition attenuates increases in insulin and leptin during short-term bed rest
Authors: Gonzalez Javier T. , Dirks Marlou L. , Holwerda Andrew M. , Kouw Imre W. K. , van Loon Luc J. C. 
Published: July 2020

Abstract
Purpose
To compare endocrine responses to intermittent vs continuous enteral nutrition provision during short-term bed rest.

Methods
Twenty healthy men underwent 7 days of bed rest, during which they were randomized to receive enteral nutrition (47%E as carbohydrate, 34%E as fat, 16%E as protein and 3%E as fibre) in a continuous (CONTINUOUS; n = 10; 24 h day−1 at a constant rate) or intermittent (INTERMITTENT; n = 10; as 4 meals per day separated by 5 h) pattern. Daily plasma samples were taken every morning to assess metabolite/hormone concentrations.

Results
During bed rest, plasma leptin concentrations were elevated to a lesser extent with INTERMITTENT vs CONTINUOUS (iAUC: 0.42 ± 0.38 vs 0.95 ± 0.48 nmol L−1, respectively; P = 0.014) as were insulin concentrations (interaction effect, P < 0.001) which reached a peak of 369 ± 225 pmol L−1 in CONTINUOUS, compared to 94 ± 38 pmol L−1 in INTERMITTENT (P = 0.001). Changes in glucose infusion rate were positively correlated with changes in fasting plasma GLP-1 concentrations (r = 0.44, P = 0.049).

Conclusion
Intermittent enteral nutrition attenuates the progressive rise in plasma leptin and insulinemia seen with continuous feeding during bed rest, suggesting that continuous feeding increases insulin requirements to maintain euglycemia. This raises the possibility that hepatic insulin sensitivity is impaired to a greater extent with continuous versus intermittent feeding during bed rest. To attenuate endocrine and metabolic changes with enteral feeding, an intermittent feeding strategy may, therefore, be preferable to continuous provision of nutrition.
This trial was registered on clinicaltrials.gov as NCT02521025.
Can low autopsy rates be increased? Yes, we can! Should postmortem examinations in oncology be performed? Yes, we should! A postmortem analysis of oncological cases
Authors: Waidhauser Johanna , Martin Benedikt , Trepel Martin , Märkl Bruno 
Published: July 2020

AbstractEver declining autopsy rates have been a concern of pathologists as well as clinicians for decades. Notably, in the field of oncology, data on autopsies and discrepancies between clinical and autoptic diagnoses are particularly scarce. In this retrospective study, we show the effect of a simple catalog of measures consisting of a different approach to obtain consent for autopsy, structured conferencing, and systematic teaching of residents, as well as a close collaboration between clinicians and pathologists on the numbers of autopsies, especially of oncological patients. Additionally, postmortem examination protocols from the years 2015 until 2019 were analyzed, regarding rates of discrepancies between clinical and autoptic causes of death in this category of patients. Autopsy numbers could be significantly increased from a minimum in 2014 (60 autopsies) to a maximum in 2018 (142 autopsies) (p < 0.0001). In the 67 autopsies of oncological cases, a high rate of 51% of major discrepancy between clinical and autoptic causes of death could be detected. In contrast to the general reported decline of autopsy rates, we present rising autopsy numbers over the past 5 years with an increasing number of oncological cases who underwent a postmortem examination. The high percentage of major discrepancies between clinical and autopsy diagnosis is in contrast to an expected decrease of major discrepancies in times of precise diagnostic methods and underlines the importance of autopsies to ensure high quality in diagnostics and therapy not only in the field of oncology.
An in vivo study exploring correlations between early-to-moderate disc degeneration and flexion mobility in the lumbar spine
Authors: Breen Alan , Mellor Fiona , Morris Andrew , Breen Alexander 
Published: July 2020

AbstractPurposeEarly disc degeneration (DD) has been thought to be associated with loss of spine stability. However, before this can be understood in relation to back pain, it is necessary to know the relationship between DD and intervertebral motion in people without pain. This study aimed to find out if early-to-moderate DD is associated with intervertebral motion in people without back pain.MethodsTen pain-free adults, aged 51–71, received recumbent and weight bearing MRI scans and quantitative fluoroscopy (QF) screenings during recumbent and upright lumbar flexion. Forty individual level and 10 composite (L2-S1) radiographic and MRI DD gradings were recorded and correlated with intervertebral flexion ROM, translation, laxity and motion sharing inequality and variability for both positions.ResultsKinematic values were similar to previous control studies. DD was evidenced up to moderate levels by both radiographic and MRI grading. Disc height loss correlated slightly, but negatively with flexion during weight bearing flexion (R =  − 0.356,p = 0.0.025). Composite MRI DD and T2 signal loss evidenced similar relationships (R =  − 0.305,R =  − 0.267) but did not reach statistical significance (p = 0.056,p = 0.096). No significant relationships between any other kinematic variables and DD were found.ConclusionThis study found only small, indefinite associations between early-to-moderate DD and intervertebral motion in healthy controls. Motion sharing in the absence of pain was also not related to early DD, consistent with previous control studies. Further research is needed to investigate these relationships in patients.
Nanosecond pulsed electrical fields enhance product recovery in plant cell fermentation
Authors: Rajabi Fatemeh , Gusbeth Christian , Frey Wolfgang , Maisch Jan , Nick Peter 
Published: July 2020

AbstractThe potential of pharmacologically active secondary plant metabolites is limited by the low yield from often rare plants, and the lack of economically feasible chemical synthesis of these complex compounds. Plant cell fermentation offers an alternative strategy to overcome these constraints. However, the efficiency of this approach is limited by intracellular sequestration of the products, such that continuous bioprocessing is not possible. As a precondition for such a, more attractive, continuous process, it is of great importance to stimulate the export of the product into the medium without impairing viability and, thus, the productivity of the cells. Using nicotine alkaloids of tobacco as a case study, an alternative strategy is explored, where nanosecond pulsed electric fields (nsPEFs) are applied for the efficient downstream recovery of the products. To maintain cell viability and allow for the further use of biomass, cells were exposed to strong (1–20 kV·cm−1), but very short (10–100 ns) electric pulses, which leads to a temporary permeabilisation of cell membranes. Using two transgenic cell lines, where two key genes involved in the metabolism of the anti-Alzheimer compound nornicotine were overexpressed, we could show that this nsPEF treatment improved the partitioning of some nicotine alkaloids to the culture medium without impairing viability, nor the synthesis of alkaloids. However, this release was only partial and did not work for nornicotine. Thus, nsPEFs produced a fractionation of alkaloids. We explain this electrofractionation by a working model considering the differential intracellular compartmentalization of nicotineic alkaloids.
Reflecting on 25 Years of Teaching Animal Law: Is it Time for an International Crime of Animal Ecocide?
Authors: Legge Debbie , Brooman Simon 
Published: July 2020

Abstract2019 marked the 25th anniversary of the introduction of Animal Law to the law degree at Liverpool John Moores University. This article examines changes in the legal protection of animals during this time and the impact this will have on research and scholarship in the law relating to animals. We examine whether the overall international treatment of animals has improved and how far the approach to the Animal Law curriculum should be influenced by the growth in concerns around climate change. In this context, we examine the development of the law of ecocide and the extent to which it addresses concerns around animal welfare across the globe. We suggest that those involved in the development of Animal Law, ethics and policy might usefully engage in a new vision of ecocide, which incorporates a clearer notion of ‘animal ecocide’. This new approach would enhance the international and national focus on animals in their own right, would recognise increasing knowledge of animal sentience and would move our responsibilities to them beyond anthropocentric approaches to environmental protection. We argue that the inclusion of a more specific reference to animal ecocide would contribute to the development of Animal Law and would lead to an enhanced relationship between Animal Law and attempts to protect the environment.
Rules as Resources: An Ecological-Enactive Perspective on Linguistic Normativity
Authors: van den Herik Jasper C. 
Published: July 2020

AbstractIn this paper, I develop an ecological-enactive perspective on the role rules play in linguistic behaviour. I formulate and motivate the hypothesis that metalinguistic reflexivity – our ability to talk about talking – is constitutive of linguistic normativity. On first sight, this hypothesis might seem to fall prey to a regress objection. By discussing the work of Searle, I show that this regress objection originates in the idea that learning language involves learning to follow rules from the very start. I propose an ecological-enactive response to the regress objection. The key move is to deny that language learning consists initially in learning rules. A child first engages in regular communicative behaviour, by learning first-order linguistic skills, and then retroactively interprets her own behaviour in normative metalinguistic terms, i.e., as being guided by rules by relying on reflexive or second-order linguistic skills. On this view, metalinguistic reflexivity enables regulation of already regular communicative behaviour, and thereby constitutes linguistic normativity. Finally, I argue that linguistic rules are resources: they are available to participants in order to (re)negotiate properties of situated language behaviour and thereby reorganize linguistic practices. The account developed in this paper thus allows us to understand the constitutive role of metalinguistic reflexivity for linguistic normativity without falling prey to the regress objection.
Ouabain Modulates the Functional Interaction Between Na,K-ATPase and NMDA Receptor
Authors: Akkuratov Evgeny E. , Westin Linda , Vazquez-Juarez Erika , de Marothy Minttu , Melnikova Aleksandra K. , Blom Hans , Lindskog Maria , Brismar Hjalmar , Aperia Anita 
Published: July 2020

AbstractThe N-methyl-D-aspartate (NMDA) receptor plays an essential role in glutamatergic transmission and synaptic plasticity and researchers are seeking for different modulators of NMDA receptor function. One possible mechanism for its regulation could be through adjacent membrane proteins. NMDA receptors coprecipitate with Na,K-ATPase, indicating a potential interaction of these two proteins. Ouabain, a mammalian cardiotonic steroid that specifically binds to Na,K-ATPase and affects its conformation, can protect from some toxic effects of NMDA receptor activation. Here we have examined whether NMDA receptor activity and downstream effects can be modulated by physiological ouabain concentrations. The spatial colocalization between NMDA receptors and the Na,K-ATPase catalytic subunits on dendrites of cultured rat hippocampal neurons was analyzed with super-resolution dSTORM microscopy. The functional interaction was analyzed with calcium imaging of single hippocampal neurons exposed to 10 μM NMDA in presence and absence of ouabain and by determination of the ouabain effect on NMDA receptor–dependent long-term potentiation. We show that NMDA receptors and the Na,K-ATPase catalytic subunits alpha1 and alpha3 exist in same protein complex and that ouabain in nanomolar concentration consistently reduces the calcium response to NMDA. Downregulation of the NMDA response is not associated with internalization of the receptor or with alterations in its state of Src phosphorylation. Ouabain in nanomolar concentration elicits a long-term potentiation response. Our findings suggest that ouabain binding to a fraction of Na,K-ATPase molecules that cluster with the NMDA receptors will, via a conformational effect on the NMDA receptors, cause moderate but consistent reduction of NMDA receptor response at synaptic activation.
On the Cauchy–Nicoletti Type Two-Point Boundary-Value Problem for Fractional Differential Systems
Authors: Marynets Kateryna 
Published: July 2020

AbstractWe deal with a system of quasilinear fractional differential equations, subjected to the Cauchy–Nicoletti type boundary conditions. The task of explicit solution of such problems is difficult and not always solvable. Thus we suggest a suitable numerical–analytic technique that allows to construct an approximate solution of the studied fractional boundary value problem with high precision.
Sustainable life cycle design aspects: how aware are material scientists?
Authors: Vink Karina 
Published: July 2020

AbstractWhen developing new materials many aspects of sustainability are relevant, especially when the ultimate goal is mass production. More efficient energy storage and transmission are important parts of a larger product life cycle design and the confines of the circular economy, including environmental and social concerns. For example, due to environmental, geopolitical, and health concerns, it is important to choose materials that are easily accessible, as opposed to materials requiring complicated extraction, storage, and transportation methods. Equally important is the abundance of the material, as the mass production and use of a product are not sustainable if its raw components are scarce. This requires material scientists to be aware of how their design affects the later life cycle stages of the materials they develop. Very few studies cover whether material scientists take these type of questions into consideration. To resolve this, material scientists were questioned on various sustainability aspects. Results show that most of the questioned scientists have little to no awareness of what effects mass production of their developed materials might have regarding greenhouse gases or the workforce, or what their material’s recyclability or longevity might be. The results indicate that these questioned material scientists are not fully aware of several imperative sustainability aspects and do not fully consider the impacts of their designs. To increase instilling and evaluating awareness of sustainability aspects on life cycle design, two improvements are: increasing sustainability education by lifelong learning, and adding sustainability concerns as a required component to grants and funding.
What is science for? The Lighthill report on artificial intelligence reinterpreted
Authors: AGAR JON 
Published: July 2020

AbstractThis paper uses a case study of a 1970s controversy in artificial-intelligence (AI) research to explore how scientists understand the relationships between research and practical applications. It is part of a project that seeks to map such relationships in order to enable better policy recommendations to be grounded empirically through historical evidence. In 1972 the mathematician James Lighthill submitted a report, published in 1973, on the state of artificial-intelligence research under way in the United Kingdom. The criticisms made in the report have been held to be a major cause behind the dramatic slowing down (subsequently called an ‘AI winter’) of such research. This paper has two aims, one narrow and one broad. The narrow aim is to inquire into the causes, motivations and content of the Lighthill report. I argue that behind James Lighthill's criticisms of a central part of artificial intelligence was a principle he held throughout his career – that the best research was tightly coupled to practical problem solving. I also show that the Science Research Council provided a preliminary steer to the direction of this apparently independent report. The broader aim of the paper is to map some of the ways that scientists (and in Lighthill's case, a mathematician) have articulated and justified relationships between research and practical, real-world problems, an issue previously identified as central to historical analysis of modern science. The paper therefore offers some deepened historical case studies of the processes identified in Agar's ‘working-worlds’ model.
Shared and specific patterns of dynamic functional connectivity variability of striato-cortical circuitry in unmedicated bipolar and major depressive disorders
Authors: Chen Guanmao , Chen Pan , Gong JiaYing , Jia Yanbin , Zhong Shuming , Chen Feng , Wang Jurong , Luo Zhenye , Qi Zhangzhang , Huang Li , Wang Ying 
Published: July 2020

AbstractBackgroundAccumulating studies have found structural and functional abnormalities of the striatum in bipolar disorder (BD) and major depressive disorder (MDD). However, changes in intrinsic brain functional connectivity dynamics of striato-cortical circuitry have not been investigated in BD and MDD. This study aimed to investigate the shared and specific patterns of dynamic functional connectivity (dFC) variability of striato-cortical circuitry in BD and MDD.MethodsBrain resting-state functional magnetic resonance imaging data were acquired from 128 patients with unmedicated BD II (current episode depressed), 140 patients with unmedicated MDD, and 132 healthy controls (HCs). Six pairs of striatum seed regions were selected: the ventral striatum inferior (VSi) and the ventral striatum superior (VSs), the dorsal-caudal putamen (DCP), the dorsal-rostral putamen (DRP), and the dorsal caudate and the ventral-rostral putamen (VRP). The sliding-window analysis was used to evaluate dFC for each seed.ResultsBoth BD II and MDD exhibited increased dFC variability between the left DRP and the left supplementary motor area, and between the right VRP and the right inferior parietal lobule. The BD II had specific increased dFC variability between the right DCP and the left precentral gyrus compared with MDD and HCs. The MDD had increased dFC variability between the left VSi and the left medial prefrontal cortex compared with BD II and HCs.ConclusionsThe patients with BD and MDD shared common dFC alteration in the dorsal striatal-sensorimotor and ventral striatal-cognitive circuitries. The patients with MDD had specific dFC alteration in the ventral striatal-affective circuitry.
GISS‐E2.1: Configurations and Climatology
Authors: Kelley Maxwell , Schmidt Gavin A. , Nazarenko Larissa S. , Bauer Susanne E. , Ruedy Reto , Russell Gary L. , Ackerman Andrew S. , Aleinov Igor , Bauer Michael , Bleck Rainer , Canuto Vittorio , Cesana Grégory , Cheng Ye , Clune Thomas L. , Cook Ben I. , Cruz Carlos A. , Del Genio Anthony D. , Elsaesser Gregory S. , Faluvegi Greg , Kiang Nancy Y. , Kim Daehyun , Lacis Andrew A. , Leboissetier Anthony , LeGrande Allegra N. , Lo Ken K. , Marshall John , Matthews Elaine E. , McDermid Sonali , Mezuman Keren , Miller Ron L. , Murray Lee T. , Oinas Valdar , Orbe Clara , García‐Pando Carlos Pérez , Perlwitz Jan P. , Puma Michael J. , Rind David , Romanou Anastasia , Shindell Drew T. , Sun Shan , Tausnev Nick , Tsigaridis Kostas , Tselioudis George , Weng Ensheng , Wu Jingbo , Yao Mao‐Sung 
Published: July 2020

AbstractThis paper describes the GISS‐E2.1 contribution to the Coupled Model Intercomparison Project, Phase 6 (CMIP6). This model version differs from the predecessor model (GISS‐E2) chiefly due to parameterization improvements to the atmospheric and ocean model components, while keeping atmospheric resolution the same. Model skill when compared to modern era climatologies is significantly higher than in previous versions. Additionally, updates in forcings have a material impact on the results. In particular, there have been specific improvements in representations of modes of variability (such as the Madden‐Julian Oscillation and other modes in the Pacific) and significant improvements in the simulation of the climate of the Southern Oceans, including sea ice. The effective climate sensitivity to 2 × CO2 is slightly higher than previously at 2.7–3.1°C (depending on version) and is a result of lower CO2 radiative forcing and stronger positive feedbacks.
Monitoring the charge-transfer process in a Nd-doped semiconductor based on photoluminescence and SERS technology
Authors: Yang Shuo , Yao Jiacheng , Quan Yingnan , Hu Mingyue , Su Rui , Gao Ming , Han Donglai , Yang Jinghai 
Published: July 2020

AbstractSurface-enhanced Raman scattering (SERS) and photoluminescence (PL) are important photoexcitation spectroscopy techniques; however, understanding how to analyze and modulate the relationship between SERS and PL is rather important for enhancing SERS, having a great effect on practical applications. In this work, a charge-transfer (CT) mechanism is proposed to investigate the change and relationships between SERS and PL. Analyzing the change in PL and SERS before and after the adsorption of the probe molecules on Nd-doped ZnO indicates that the unique optical characteristics of Nd3+ ions increase the SERS signal. On the other hand, the observed SERS can be used to explain the cause of PL background reduction. This study demonstrates that modulating the interaction between the probe molecules and the substrate can not only enhance Raman scattering but also reduce the SERS background. Our work also provides a guideline for the investigation of CT as well as a new method for exploring fluorescence quenching.
Correction: Cultivation and characterization of Candidatus Nitrosocosmicus exaquare, an ammonia-oxidizing archaeon from a municipal wastewater treatment system
Authors: Sauder Laura A , Albertsen Mads , Engel Katja , Schwarz Jasmin , Nielsen Per H , Wagner Michael , Neufeld Josh D 
Published: July 2020

Abstract
An amendment to this paper has been published and can be accessed via a link at the top of the paper.