bluuebunny/crossref_metadata_2025_split huggingface dataset:
Massive Restrain of Cytotoxic B cells in the Peripheral Blood During Fingolimod and Natalizumab Treatments in Multiple Sclerosis Patients
Authors: Boldrini Vinícius de Oliveira , Quintiliano Raphael Patrício da Silva , Moraes Adriel dos Santos , Stella Carla , Longhini Ana Leda Figueiredo , Santos Irene , de Andrade Marília , Damasceno Alfredo , Carneiro Rafael Paternò Dias , Brandão Carlos Otávio , Farias Alessandro dos Santos , Santos Leonilda Maria Barbosa 
Published: June 2020

Abstract
Background
Recently, the success of anti-CD20 monoclonal antibody therapy brought a new light over the role of B cells in multiple sclerosis (MS) pathogenesis. Due to the expression pattern of CD20 during B cells ontogeny, this role seems to be extended beyond the antibodies' production and secretion. Therefore, here we investigated whether not only classical cytotoxic CD8+ T lymphocytes but also non-classical cytotoxic B cells may occur in the peripheral blood from relapsing-remitting MS (RRMS) patients.
Methods
104 RRMS patients during different treatment and 58 healthy donors were studied. CD19, GzmB, Runx3 and CD49d expression was assessed by flow cytometry analyses.
Results
Patients treated with Natalizumab (NTZ) showed an increased percentage of CD8+GzmB+ when compared to other MS therapies, untreated RRMS patients and healthy volunteers. Similarly, and unexpected, massive cytotoxic behavior of B cells CD19+GzmB+ was observed in RRMS patients during Fingolimod (FTY) and NTZ therapies when compared to Glatiramer, Interferonβ, untreated MS patients and healthy donors.
Conclusions
During different MS treatments, B cells exhibit cytotoxic behavior resembling CD8+ T lymphocytes. This data suggest a possible involvement of “cytotoxic” B cells during MS pathology. Monitoring cytotoxic subsets might become an available marker for the risk of relapses and even for accessing therapeutic effectiveness in MS patients.
Lipid metabolic gene-wide profile and signature of lung adenocarcinoma
Authors: Li Jinyou , Li Qiang , Su Zhenyu , Sun Qi , Zhao Yong , Feng Tienan , Jiang Jiayuan , Ma Haitao , Zhang Feng 
Published: June 2020

Abstract
Background
Lung cancer is a worldwide cancer with high morbidity and mortality. More and more evidence shows that the disorder of lipid metabolism is the key to the development of cancer, and analysis of lipid-related genes may lead to diagnosis and prognostic biomarkers related to lung cancer.
Methods
In this study, we performed the differentially expressed analysis of 1045 lipid metabolism-related genes between LUAD tumors and normal tissues in the TCGA-LUAD cohort. Then the bioinformatic analysis of DEGs was showed. PPI networks and cytoHubba APP determine hub genes. The association between hub genes and overall survival was evaluated by Kaplan-Meier Plotter. To predict the prognosis of LUAD patients, a nomogram was built, the nomogram was validated by another cohort (GSE13213).
Results
Finally, a total of 217 lipid metabolism-related DEGs were detected in LUAD. They were significantly enriched in Glycerophospholipid metabolism, fatty acid metabolic process, and Eicosanoid Signaling. Then we identified 6 hub genes through PPI network and cytoHubba, including INS, LPL, HPGDS, DGAT1, UGT1A6, and CYP2C9. The high expression of CYP2C9, UGT1A6, and INS, whereas low expressions of DGAT1, HPGDS, and LPL, were associated with worse OS for 1925 LUAD patients. Based on the nomogram, we found that the high-risk score group had a worse OS, and the validated cohort had the same result.
Conclusion
In conclusion, we generated a lipid metabolic transcriptome-wide profile of LUAD patients and found that significant lipid metabolic pathways were correlated with the LUAD. Furthermore, we constructed a signature of six lipid metabolic genes, which significantly associated with diagnosis and prognosis of LUAD patients. The gene signature can be used as a biomarker for LUAD.
A Comparative Protein Profile of Virgin and Mated Male of Leucinodes Orbonalis Accessory Gland
Authors: Saraswathi Saraswathi , B.S Chaitra , Kiran Tannavi , R Mamtha , R Sowrabha , Rao Karthik V , doddamane manjulakumari 
Published: June 2020

Abstract
Male accessory gland (MAG) proteins that are secretory in nature delivered to females, along with sperms affects female reproductive physiology and behavior. In our study, proteomic approaches were employed to identify the MAG proteins of L. orbonalis, a monophagous and most destructive pest on brinjal. A set of 117 spots in virgin MAG and 186 in mated MAG were obtained from 2-D gel electrophoresis. The differentially expressed MAG proteins after mating in comparison with the virgin male were 14 upregulated and 16 down-regulated. We have used MALDI- MS to identify the 13 unique proteins within the virgin MAG of the L. orbonalis and analyzed with the Swiss-Prot database using a Mascot search engine. The proteins were identified as proteolysis regulators, lipid transporter, olfactory protein, metabolism, DNA binding, and hexamerins. This is the first report on proteome analysis of MAG of the Leucinodes orbonalis.
Effects of ascorbic acid supplementation on immune status in healthy women following a single bout of exercise
Authors: Bunpo Piyawan , Chatarurk Amonphat , Intawong Kodchaporn , Naosuk Kananek , Klangsinsirikul Phennapha 
Published: June 2020

Abstract
Background: This study was designed to determine the effects of ascorbic acid (AA) supplementation on immune status following a single bout of exercise. Materials/Methods: In a crossover design with a 1 week wash-out period, 20 healthy sedentary women performed 30 minutes moderate-intensity cycling with (1,000AA) or without (0AA) ingesting 1,000 mg of AA daily for 1 week. Blood samples were taken immediately before, immediately after and 24 hours post-exercise to determine the oxidative stress markers, hematological parameters, immunophenotyping of peripheral blood lymphocytes and neutrophil phagocytic function with Candida albicans. Results: Moderate-intensity exercise in subjects ranged in age from 21 to 23 years, showed no significant changes in oxidative stress markers in both cohorts. Plasma total creatine kinase was increased immediately after exercise and returned to baseline at 24 h post-exercise in both cohorts. Subjects ingesting 1,000 mg AA demonstrated significant higher level of plasma AA at pre-exercise and post-exercise as compared with the same time point in 0AA group. White blood cell and absolute neutrophil counts were increased immediately after exercise and returned to baseline at 24 h post-exercise in both cohorts. Exercise resulted in increased lymphocyte count, CD4+ T cells, CD8+ T cells, CD45+/CD3-/CD4- cells, CD45+/CD3+/CD4- cells, CD45+/CD3-/CD8- cells, CD45+/CD3+/CD8- cells and CD45+/CD3-/CD8+ cells immediately after exercise (p<0.05) with a return to baseline at 24 hours in 0AA group. AA supplement mitigated effects of exercise on CD4+ T cells and CD45+/CD3-/CD8- cells. No significant change in neutrophil phagocytic function were observed when incubated with low or high concentrations of C. albicans in both cohorts. Conclusion: A single bout of exercise induced muscle damage and transient changes in neutrophil count as well as lymphocyte subpopulations in sedentary women. Ascorbic supplementation does not show beneficial effect to the moderate-intensity exercise.
Genetic Characterization of Plasmodium vivax Isolates from Pakistan Using Circumsporozoite Protein (PvCSP) and Merozoite Surface Protein-1 (PvMSP-1) Genes as Genetic Markers
Authors: Bibi Zainab , Fatima Anam , Rani Rehana , Maqbool Ayesha , Khan Samea , Naz Shumaila , Waseem Shahid 
Published: June 2020

Abstract
Background Plasmodium vivax contribute over 70% malaria burden in Pakistan. Limited data exist on various aspects including genetic diversity of the parasite as compared to other parts of the world. The information about extent of genetic diversity assists to understand the transmission patterns of the parasite in human host. The current study was designed to understand population divergence of Plasmodium vivax in Pakistan using circumsporozoite protein and merozoite surface protein-I genes as molecular markers.Methods PvCSP and PvMSP-1 specific PCR and DNA sequencing were carried out for 150 blood samples collected from Islamabad and Rawalpindi, Pakistan. Genetic diversity was analysed using ChromasPro, ClustalW, MEGA7 and DnaSP v.5 programs.Results The PCR for PvCSP and PvMSP-1 genes was carried out for 150 P. vivax isolates resulting the PCR products ranging from 900 to 1100 bp for PvCSP gene and ~ 400 bp for PvMSP-1 gene. Majority (93%; 121/150) of the P. vivax isolates were of VK210 variant type and only 9 isolates were found of VK247 variant type based on PvCSP gene. Out of the numerous peptide repeat motifs (PRMs) detected, GDRADGQPA (PRM1) and GDRAAGQPA (PRM2) were more extensively dispersed among the P. vivax isolates. Partial sequences (~ 400 bp) at the N-terminal of PvMSP-1 gene depicted high level of diversity.Conclusion High-level genetic diversity based on PvCSP and PvMSP-1 genes was observed in clinical isolated in the study area. Parasite typing is essential in predicting pattern of antigenic variations, drug resistance and for effective drug and vaccine designing and development which can further evaluate for malaria control and eradication at individual and community level. The base-line data presented here warrant future studies to investigate more into the genetic diversity of P. vivax with large sample size from across the country for better understanding of the vivax malaria transmission patterns.
Effectiveness of The Innovative 1,7-Malaria Reactive Community-based Testing and Response (1, 7-mRCTR) Approach on Malaria Burden Reduction in Southeastern Tanzania
Authors: Mlacha Yeromin P. , Wang Duoquan , Chaki Prosper P. , Gavana Tegemeo , Zhou Zhengbin , Michael Mihayo G. , Khatib Rashid , Chila Godlove , Msuya Hajiran M. , Chaki Exavery , Makungu Christina , Lin Kangming , Tambo Ernest , Rumisha Susan F. , Mkude Sigbert , Mahende Muhidin K. , Chacky Frank , Vounatsou Penelope , Tanner Marcel , Masanja Honorati , Aregawi Maru , Hertzmark Ellen , Xiao Ning , Abdulla Salim , Zhou Xiao-Nong 
Published: June 2020

Abstract
Background: In 2015, a China-UK-Tanzania tripartite pilot project was implemented in south-eastern Tanzania to explore a new model for reducing malaria burden and possibly scaling-out the approach into other malaria endemic countries. The 1,7-malaria Reactive Community-based Testing and Response (1,7-RCTR) which is a locally-tailored approach for reporting febrile malaria cases in endemic villages was developed to stop transmission and plasmodium life-cycle. The (1,7-RCTR) utilizes existing health facility data and locally trained community health workers to conduct community-level testing and treatment. Methods: The pilot project was implemented from September 2015 to June 2018. Matched malaria incidence pairs of control and intervention wards were chosen. The latter arm was selected for the 1,7-mRCTR approach leaving control wards relying on existed programs. The 1,7-mRCTR activities included community testing and treatment of malaria infection. Malaria case-to-suspect ratios at health facilities (HF) were aggregated by villages, weekly to identify the village with the highest ratio. Community-based mobile test stations (cMTS) were used for conducting mass testing and treatment. Random household surveys were done in the control and intervention wards before (baseline) and after (endline) the program. The primary outcome was the baseline and endline difference of malaria prevalence in the control and intervention wards measured by the interaction term of ‘time’ (post vs. pre) and group in a logistic model. We also studied the malaria incidence reported at the health facilities during the intervention.Results: Overall 85 rounds of 1,7-mRCT conducted in the intervention wards significantly reduced the odds of malaria infection by 66% (adjusted OR 0.34, 95%CI 0.26,0.44, p<0001) beyond the effect of the standard programs. Malaria prevalence in the intervention wards declined by 81% (from 26% (95% CI, 23.7, 7.8), at baseline to 4.9% (95% CI, 4.0,5.9) at endline). Villages receiving the 1,7-mRCT had a case ratio decreased by over 15.7% (95%CI, -33, 6) compared to baseline.Conclusion: The 1,7-mRCTR approach reduced significantly the malaria burden in the areas of moderate and high transmission in southern Tanzania. This locally-tailored approach could accelerate malaria control and elimination efforts. The results provide the impetus for further evaluation of the effectiveness and scaling up of this type of approach in other high malaria burden countries in Africa, including Tanzania.
HSP22 (HSPB8) positively regulates PGF2α-induced synthesis of interleukin-6 and vascular endothelial growth factor in osteoblasts
Authors: Kuroyanagi Gen , Sakai Go , Otsuka Takanobu , Yamamoto Naohiro , Fujita Kazuhiko , Kawabata Tetsu , Matsushima-Nishiwaki Rie , Kozawa Osamu , Tokuda Haruhiko 
Published: June 2020

Abstract
Background: Heat shock protein 22 (HSP22) belongs to class I of the small HSP family that displays ubiquitously expression including in osteoblasts. We previously demonstrated that prostaglandin F2α (PGF2α), a potent bone remodeling factor, induces the synthesis of interleukin-6 (IL-6) and vascular endothelial growth factor (VEGF) via p44/p42 mitogen-activated protein (MAP) kinase and p38 MAP kinase in osteoblast-like MC3T3-E1 cells. In the present study, we investigated whether HSP22 is implicated in the PGF2α-induced synthesis of IL-6 and VEGF, and the mechanism in MC3T3-E1 cells. Methods: MC3T3-E1 cells were transfected with HSP22-siRNA. IL-6 and VEGF release was assessed by ELISA. Phosphorylation of p44/p42 MAP kinase and p38 MAP kinase was detected by Western blotting. Results: The PGF2α-induced release of IL-6 in HSP22 knockdown cells was significantly suppressed compared with that in the control cells. HSP22 knockdown also reduced the VEGF release by PGF2α. Phosphorylation of p44/p42 MAP kinase and p38 MAP kinase was attenuated by HSP22 down-regulation. Conclusions: Our results strongly suggest that HSP22 acts as a positive regulator in the PGF2α-induced synthesis of IL-6 and VEGF in osteoblasts.
Self-Determination Theory Interventions Versus Usual Care in People With Diabetes: A Protocol for a Systematic Review With Meta-Analysis and Trial Sequential Analysis
Authors: Mathiesen Anne Sophie , Rothmann Mette Juel , Zoffmann Vibeke , Jakobsen Janus Christian , Gluud Christian , Lindschou Jane , Due-Christensen Mette , Rasmussen Bodil , Marqvorsen Emilie , Thomsen Thordis 
Published: June 2020

Abstract
Background Existing self-management and behavioural interventions for diabetes vary widely in their content, and their sustained long-term effectiveness is uncertain. Autonomy supporting interventions may be a prerequisite to achieve ‘real life’ patient engagement and more long-term improvement through shared decision making and collaborative goal setting. This is the goal of self-determination theory and guided self-determination interventions. Self-determination theory has been reviewed but without assessing both benefits and harms and accounting for the risk of random errors using trial sequential analysis. The guided self-determination has not yet been systematically reviewed. The aim of this protocol is to investigate the benefits and harms of self-determination theory-based interventions versus usual care in adults with diabetes.Methods/design We will conduct the systematic review following The Cochrane Collaboration guidelines. This protocol is reported according to the PRISMA checklist. A comprehensive search will be undertaken in the CENTRAL, MEDLINE, EMBASE, LILACS, PsycINFO, SCI-EXPANDED, CINAHL, SSCI, CPCI-S, and CPCI-SSH to identify relevant trials. We will include randomised clinical trials assessing interventions theoretically based on guided self-determination or self-determination theory provided face-to-face or digitally by any health care professional in any setting. The primary outcomes will be quality of life, mortality, and serious adverse events. The secondary will be diabetes distress, depressive symptoms, and adverse events not considered serious. Exploratory outcome will be glycated haemoglobin. Outcomes will be assessed at the end of the intervention and at maximum follow-up. The analyses will be performed using Stata version 16 and Trial Sequential Analysis. Two authors will independently screen, extract data from, and perform risk of bias assessment of included studies using the Cochrane risk of bias tool. Certainty of the evidence will be assessed by GRADE.Discussion Self-determination theory interventions aim to promote a more autonomous patient engagement and are commonly used. It is therefore needed to evaluate the benefit and harms according to existing trials. Systematic review registration The protocol has been registered in PROSPERO ID nr. 181144 (submitted to PROSPERO 24th April 2020)
Quantitative visualization of photosynthetic pigments in tea leaves based on Raman spectroscopy and calibration model transfer
Authors: Zeng Jianjun , Zhang Shuai , Luo Wei , Sha Junjing , Huang Yifeng , Liu Xuemei , Zhan Baishao , Zhang Hailiang , Li Xiaoli 
Published: June 2020

Abstract
Background: Photosynthetic pigments participating in the absorption, transformation and transfer of light energy play a very important role in plant growth. While, the spatial distribution of foliar pigments is an important indicator of environmental stress, such as pests, diseases and heavy metal stress. Results: In this paper, in situ quantitative visualization of chlorophyll and carotenoid was realized by combining the Raman spectroscopy with calibration model transfer, and a laboratory Raman spectral model was successfully extended to a portable field spectral measurement. Firstly, a nondestructive and fast model for determination of chlorophyll and carotenoid in tea leaf was established based on confocal micro-Raman spectrometer in the laboratory. Then the spectral model was extended to a real-time foliar map scanning spectra of a field portable Raman spectrometer through calibration model transfer, and the spectral variation between the confocal micro-Raman spectrometer in the laboratory and the portable Raman spectrometer were effectively corrected by the direct standardization (DS) algorithm. The portable map scanning Raman spectra of the tea leaves after the model transfer were got into the established quantitative determination model to predict the concentration of photosynthetic pigments at each pixel of the tea leaves. The predicted photosynthetic pigments concentration of each pixel was imaged to illustrate the distribution map of foliar pigments. Statistical analysis showed that the predicted pigment contents were highly correlated with the real contents. Conclusions: It can be concluded that the Raman spectroscopy was applicable for in situ, non-destructive and rapid quantitative detecting and imaging of photosynthetic pigment concentration in tea leaves, and the spectral detection model established based on the laboratory Raman spectrometer can be applied to a portable field spectrometer for quantitatively imaging of the foliar pigments.
Comparison of different bacteriological testing strategies and factors for bacteriological confirmation among pulmonary TB patients: a retrospective study in Tianjin, China, 2017-2018
Authors: Zhang Guoqin , Zhang Yuhua , Chen Mingting , Zhang Fan 
Published: June 2020

Abstract
Background
Bacteriological confirmation rate among notified pulmonary TB patients in China is among the lowest in the world. This study was to understand the yield of bacteriological confirmation using different testing strategies and patient-level factors associated with bacteriological confirmation among pulmonary TB patients in Tianjin, China between 2017 and 2018.
Methods
A retrospective study was conducted, enrolling pulmonary TB patients reported to National TB Information Management System (TIMS) in Tianjin during 2017–2018. Bacteriological confirmation was defined as a positive result by any of the followings: smear microscopy, culture, or nucleic acid amplification test. Individual characteristics were compared between patients with positive and negative bacteriological results using contingency tables and χ2 test. Multivariable logistic regression was applied to analyze factors associated with bacteriological confirmation, calculating adjusted odds ratios (aOR) and 95% confidence intervals (CI) (α = 0.05).
Results
Of 6,364 reported patients, 4,181 (65.7%) were bacteriologically confirmed. Positivity proportion was 43.1% (2,746/6,364) for smear microscopy, 57.7% (3,380/5,853) for culture, 61.7% (1,608/2,605) for Xpert MTB/RIF and 73.4% (1,824/2,484) for combination of the three. Occupation as un-employed (aOR = 1.5, 95% CI: 1.0-2.2) and a farmer (aOR = 1.7, 95% CI: 1.1–2.8) compared with a student; diagnosis by inpatient hospitals compared with TB clinics (aOR = 3.4, 95% CI: 2.6–4.4); delay ≥ 2 weeks for TB care (aOR = 1.4, 95% CI: 1.1–1.8); cough (aOR = 2.2, 95% CI: 1.8–2.8); blood sputum (OR = 1.5, 95% CI: 1.0-2.2); cavitation on chest X-ray (OR = 3.3, 95% CI: 2.5–4.3); bilateral lung lobes affected (aOR = 1.7, 95% CI: 1.4–2.2) were factors associated with bacteriological confirmation.
Conclusions
Combination tests was an effective way to improve bacteriological confirmation among pulmonary TB patients. Being unemployed, farmers, delay for TB care, and more severe in TB condition were factors associated with bacteriological confirmation among the patients. We recommend combination of bacteriological tests and sputum collection intervention to improve bacteriological confirmation for pulmonary TB patients, especially who are in early stage of the disease or with conditions tend to be bacteriologically negative.
Detection of Subclinical Skin Manifestation in Patients With Psoriasis and Psoriatic Arthritis by Fluorescence Optical Imaging
Authors: Schmidt Angelique , Glimm Anne-Marie , Haugen Ida Kristin , Hoff Paula , Schmittat Gabriela , Burmester Gerd Ruediger , Klotsche Jens , Ohrndorf Sarah 
Published: June 2020

Abstract
Objectives:To investigate the frequency of subclinical skin inflammation in both hands by fluorescence optical imaging (FOI) in patients with psoriasis/psoriatic arthritis (Pso/PsA) vs. rheumatoid arthritis (RA) and healthy individuals, and to correlate these findings with cardiovascular (CV) risk factors.Patients and Methods:The FOI scans were analyzed retrospectively to detect clinically invisible skin enhancement (0-3 scale) in both hands without relationship to underlying joints or blood vessels. We further characterized the FOI patterns and sorted the scans into groups based on the assumed diagnosis (Pso/PsA, RA and healthy controls), which was compared with the physician’s diagnosis. Furthermore, the associations between CV risk factors and imaging findings were investigated by regression analyses.Results:We included FOI scans of patients with Pso/PsA (n=80), RA (n=78) and healthy controls (n=25). Subclinical skin enhancement on the back of their hands was more common in Pso/PsA (72.5%) than in RA patients (20.5%) and healthy individuals (28.0%) (p<0.001). Based on the FOI pattern, the majority of patients with Pso/PsA (72.5%), RA (76.9%) and healthy controls (68.0%) were classified correctly using the physician-based diagnosis as reference (overall agreement of 74%, kappa=0.57). No CV risk factors except body weight (kg) was associated with subclinical skin enhancement (OR 1.04, 95% CI 1.02-1.06; p<0.001).Conclusion:Subclinical subdermal skin inflammation was common in Pso/PsA patients using FOI. Based on the FOI pattern, most patients with Pso/PsA and were classified with the correct diagnosis. We demonstrated an important influence of the body weight on our FOI results. FOI may be a helpful novel tool to study microcirculation in rheumatic diseases with skin involvement.