bluuebunny/crossref_metadata_2025_split huggingface dataset:
A double-layer PLGA/CoI-MeHA tissue engineering scaffold for urethral reconstruction
Authors: Chang Mingyang , Tan Qinyuan , Bian Ge , Zhang Ming , Lv Jianing , Su Junjie , Wang Xiaoqing 
Published: February 2025

IntroductionUrethral injury caused by various reasons usually leads to urethral stricture. And severe urethral stricture can further induce complications such as bladder stones, fistulas, sepsis, and even renal failure. At present, surgical methods such as urethral reconstruction and end-to-end anastomosis are commonly used to solve this problem. But this treatment method often has a high recurrence rate. So simply relying on the repair of surrounding autologous tissue cells to reconstruct the urethra is difficult to achieve long-term stability, and constructing a suitable urethral graft is an effective and feasible solution.MethodsHere, we designed and prepared a double-layer PLGA/CoI-MeHA tissue engineering scaffold to better simulate the natural anatomy of the urethra and achieve urethral tissue regeneration and reconstruction in patients with urethral stricture and Hypospadias caused by various reasons. The double-layer tissue engineering scaffold was generated using electrospinning and light curing technology.ResultsThrough electrospinning and light curing technology, we successfully screened the PLGA/CoI (7:3) electrospun membrane and MeHA (40.72%) hydrogel. Furthermore, we successfully prepared PLGA/CoI-MeHA bilayer urethral stents loaded with rabbit urethral smooth muscle cells and rabbit urethral epithelial cells, respectively, and achieved favorable results for urethral defect repair and urethral reconstruction in rabbits. The mechanical characterization of the scaffold indicates that it has sufficient mechanical strength to meet experimental and clinical needs. In addition, it showed satisfactory biocompatibility in cell experiments and in the in vitro degradation experiments. The double-layer urethral stents demonstrated exceptional performance in repairing urethral defects in rabbits.DiscussionWe had successfully designed and prepared a double-layer PLGA/CoI-MeHA tissue engineering scaffold. The stent displayed sufficient mechanical strength, good biocompatibility and degradation characteristics, and effectively simulated the natural anatomy of urethra, achieving satisfactory urethral defect reconstruction results.
Progress in understanding Legg–Calvé–Perthes disease etiology from a molecular and cellular biology perspective
Authors: Zheng Xinda , Dong Zhuqing , Ding Xiaofei , Huang Qian , Tang Shengping , Zhang Yuchen , Li Boxiang , Liao Shijie 
Published: February 2025

Legg–Calvé–Perthes disease (LCPD) is a hip disease caused by ischemia of the femoral epiphysis in children, which occurs in children aged 4–8 years (mean 6.5 years), with a male-to-female ratio of about 4:1. The disease has been reported for more than 100 years, but its etiology has not been elucidated. In recent years, a considerable amount of research has been carried out on the etiology of the disease, and the development of the disease is believed to involve a variety of molecular biological alterations, such as the COL2A1 mutation, which may be one of the causes of necrotic collapses of the epiphyseal cartilage matrix in LCPD. Tissue factor V Leiden mutation and insulin-like growth factor (IGF-1) abnormalities have also been reported in LCPD, but most theories need further confirmation. The in-depth study of LCPD cell biology has facilitated the suggestion regarding structural and/or functional abnormalities of microvascular endothelial cells in LCPD. This conjecture is supported by epidemiological and clinical evidence. Abnormal activation of osteoclasts, ischemic damage to epiphyseal cartilage, and activation of the bone marrow immune system all play important roles in the onset and progression of the disease. In this paper, we review the previous basic studies on LCPD and give an overview from the molecular biology and cell biology perspectives.
Monitoring skeletal muscle oxygen saturation kinetics during graded exercise testing in NCAA division I female rowers
Authors: Eserhaut Drake A. , DeLeo Joseph M. , Provost Jessica A. , Ackerman Kathryn E. , Fry Andrew C. 
Published: February 2025

PurposeThe purpose of this study was to analyze changes in skeletal muscle oxygen saturation (SmO2) kinetics during exercise in female rowers both acutely and longitudinally in relation to blood lactate (BLa). We also aimed to determine the agreement and statistical equivalence between physiological thresholds derived from SmO2 and BLa kinetics.MethodsTwenty-three female NCAA Division I rowers were tested throughout the 2023–2024 academic year. Of these, 11 athletes completed at least two near-infrared spectroscopy (NIRS)-equipped GXTs, with physiological data analyzed for longitudinal changes. A 7x4-min discontinuous GXT protocol was performed by all athletes. First and second SmO2 breakpoints (SmO2BP1 and SmO2BP2) were estimated via piecewise linear regression modeling, and BLa thresholds (LT1 and LT2) were calculated using ADAPT software. Paired-samples t-tests assessed differences, and equivalence was tested using two one-sided tests (TOST). Agreement was determined using Bland-Altman analysis yielding mean differences (MD) and 95% limits of agreement (LoA). Intraclass correlation coefficients (ICC2,1) were also calculated.ResultsNo difference was found between SmO2BP2 and LT2 (MD = −5.76W [95% LoA = −38.52 to 22.25W], p = 0.134), moderate-to-good levels of agreement (ICC2,1 = 0.67 [95% CI: 0.36–0.85], p &lt; 0.001), and no statistical equivalence (p = 0.117). This was not the case for SmO2BP1 and LT1, with NIRS significantly underestimating LT1 (MD = −8.14W [95% LoA = −38.90 to 27.37W], p = 0.026), poor-to-moderate agreement (ICC2,1 = 0.24 [95% CI: −0.13–0.58], p = 0.10), and no statistical equivalence (p = 0.487). Additionally, SmO2 recovery kinetics (SmO2resat) during 1-min rest intervals increased in response to graded increases in exercise intensity (p &lt; 0.001, η2p = 0.71), with higher intensities appearing to blunt this effect (step 6 – step 7: MD = −0.16%⋅s-1, p = 0.69). No statistically significant changes were observed in LT’s or SmO2BP’s throughout the 2023–2024 season.ConclusionIn female collegiate rowers, NIRS may be a tool that compliments BLa testing when determining the second lactate threshold (i.e., LT2). However, significant inter-individual variablility exists between SmO2BP2 and LT2 paired with a lack of statistical equivalence suggest the two are not interchangeable. While not a standalone replacement, if used in combination with traditional BLa testing methods NIRS may be a complimentary tool that helps inform individual athlete training zone prescription.
Effects of broad-leaved grass inhibitors and nitrogen fertilizer on seed production Elymus nutans in alpine meadow of the Qinghai-Tibet Plateau
Authors: Lu Xin , Qi Juan , Su Junhu , Liu Yanjun , Zhang Aolong 
Published: February 2025

IntroductionThe alpine meadows of the Tibetan Plateau play a crucial role in the grassland ecosystem. However, due to the rapid growth and strong competitiveness of broad-leaved grasses, the nutritional resources and living space available for Gramineae species are severely restricted in this region. Broad-leaved grasses and noxious weeds have evolved into dominant population, severely limiting grassland production in alpine meadows. A shortage of premium seeds limits grassland ecosystem restoration efforts. Elymus nutans is regarded as a pioneer plant for restoring degraded grassland into meadows dominated by grasses, and for developing cultivated grassland in the Tibetan Plateau region, and the demand for native seeds of E. nutans is increasing.MethodsTherefore, this study investigated the effect of combinations of four levels of a broad-leaved grass inhibitor (0, 0.9, 1.5, and 2.1 kg·hm-2) crossed with four levels of nitrogen fertilizer (0, 75, 150, and 225 kg·hm-2) on seed production of E. nutans in Gannan alpine meadow of the Qinghai-Tibet Plateau.ResultsWe observed that the grass inhibitor significantly (p &lt; 0.05) influenced on fertile tillers (FT), spikelets per fertile tiller (SFT), seeds per spikelet (SS) and panicle length (PL), but not florets per spikelet (FS) (p = 0.145). Nitrogen fertilizer significantly influenced on FT, FS, SS, and PL (p &lt; 0.001), but not SFT (p = 0.068). The interaction of the grass inhibitor and nitrogen fertilizer had no significant effect on any of these seed yield components (p &gt; 0.05). Both the grass inhibitor and nitrogen fertilizer significantly influenced all indicators of seed production (p &lt; 0.001), increasing their values in a dose-dependent manner. Moreover, their interaction proved significant for all indicators (p &lt; 0.001), except for actual seed yield (p &gt; 0.05), demonstrating their synergistic effects. The maximum thousand seed weight (4.66 g) and actual seed yield (365 kg·hm-2) were observed at the highest doss of 2.1 kg·hm-2 of grass inhibitor and 225 kg·hm-2 of nitrogen fertilizer, which were 1.85-fold and 2.94-fold of the control, respectively. Furthermore, significantly positive correlations were observed among seed yield and all yield components. Pathway analysis showed that FT made significant direct contributions to the seed yield.DiscussionThis approach (using broad-leaved grass inhibitors and nitrogen fertilizer) effectively reduced competition from broad-leaved grasses and increased the proportion of E. nutans in the plant community composition, thus alleviating the shortage of E. nutans seeds for grassland ecological restoration.
Validation of stay-green and stem reserve mobilization QTLs: physiological and gene expression approach
Authors: Taria Sukumar , Arora Ajay , Kumar Sudhir , Krishna Hari , Meena Shashi , Singh Biswabiplab , China Malakondaiah Animireddy , S. Kousalya , Padaria Jasdeep Chatrath , Singh Pradeep Kumar , Alam Badre , Kumar Sushil , Arunachalam Ayyanadar 
Published: February 2025

IntroductionAbiotic stress significantly reduces the wheat yield by hindering several physiological processes in plant. Stay-green (SG) and stem reserve mobilization (SRM) are the two key physiological traits, which can contribute significantly to grain filling during stress period. Validation of genomic regions linked to SG and SRM is needed for its subsequent use in marker-assisted selection in breeding program.MethodsUsing a physiological and gene expression approach, quantitative trait loci (QTLs) for stay-green (SG) and stem reserve mobilization (SRM) were validated in a pot experiment study using contrasting recombinant inbred lines including its parental lines (HD3086/HI1500) in wheat. The experiment was laid down in a completely randomized design under normal (control, drought) and late sown (heat and combined stress) conditions during the 2022-2023 rabi season. Drought stress was imposed by withholding irrigation at the anthesis stage, whereas heat stress was imposed by 1-month late sowing compared to the normal sowing condition. Combined stress was imposed by 1-month late sowing along with restricted irrigation at the flowering stage. Superior lines (HDHI113 and HDHI87) had both SG and SRM traits, whereas inferior lines (HDHI185 and HDHI80) had contrasting traits, i.e., lower SG and SRM traits. HD3086 and HI1500 had SG and SRM traits respectively. Potential candidate genes were identified based on the flanking markers of the mapped QTLs using the BioMart tool in the Ensembl Plants database to validate the identified QTLs. Real-time gene expression was conducted with SG-linked genes in the flag leaf and SRM-linked genes in the peduncle.Results and DiscussionIn this study, HDHI113 and HDHI87 showed higher expression of SG-related genes in the flag leaf under stress conditions. Furthermore, HDHI113 and HDHI87 maintained higher chlorophyll a content of 7.08 and 6.62 mg/gDW, respectively, and higher net photosynthetic rates (PN) of 17.18 and 16.48 µmol CO2/m2/s, respectively, under the combined stress condition. However, these lines showed higher expression of SRM-linked genes in the peduncle under drought stress, indicating that drought stress aggravates SRM in wheat. HDHI113 and HDHI87 recorded higher 1,000-grain weights and spike weight differences under combined stress, further validating the identified QTLs being linked to SG and SRM traits. Henceforth, the identified QTLs can be transferred to developed wheat varieties through efficient breeding strategies for yield improvement in harsh climate conditions.
Effectiveness of brief online mindfulness-based intervention on different types of mobile phone addiction: mechanisms of influence of trait mindfulness
Authors: Zhang Zhiyu , Wu Lei , Lu Chu , Guan Tianming 
Published: February 2025

IntroductionIt is common for college students to suffer from mobile phone addiction, which can seriously affect their physical and mental health. The current study looked at the impact of a brief online mindfulness-based intervention (MBI) on mobile phone addiction to address this problem. The mechanisms behind the influence of trait mindfulness (TM) on this process were also investigated in the current study.MethodsSubjects were split into two groups for a randomized controlled trial: an experimental group and a control group. During brief online MBI, online assessments of mobile social networking addiction (MSNA), mobile game addiction (MGA), mobile information acquisition addiction (MIAA), and mobile short-form video addiction (MSVA) as well as TM were completed. The present study examined the effectiveness of the brief online MBI with a two-factor repeated measures ANOVA and explored the effects of TM on the four types of mobile phone addiction with hierarchical linear modeling (HLM) after a brief online MBI.ResultsResults revealed that the experimental group receiving brief online MBI showed a significant decrease in MSNA, MGA, MIAA, and MSVA at the post-test level compared to the pre-test. TM negatively predicted MSNA, MGA, MIAA, and MSVA.DiscussionThe present study found that the brief online mindfulness-based intervention can effectively reduce four types of mobile phone addiction. In addition, the present study revealed that as the levels of TM increased, all four types of mobile phone addiction decreased.
Development and validation of a secondary school classroom engagement instrument in math and science in the Ethiopian context
Authors: Berhanu Alemayehu , Semela Tesfaye , Moges Belay 
Published: February 2025

IntroductionClassroom engagement is the most influential variable in predicting students’ academic achievement. However, a valid and reliable instrument for measuring students’ classroom engagement in the Ethiopian context remains unexplored. This study aimed to develop and validate an Ethiopian secondary school student classroom engagement instrument.MethodA total of 119 items were selected through a rigorous literature review, and 40 of these items were initially drafted on the basis of expert judgment. These selected items were subsequently tested on 1,771 students: 383 for exploratory factor analysis (EFA), 1,346 for confirmatory factor analysis (CFA), and 42 for test–retest reliability. The internal consistency of the full scale and subscales of this tool were computed via Cronbach’s alpha. The measurements of invariance across gender and grade levels were analyzed to determine the level of equivalence of the instrument.ResultsThe findings revealed that this tool is valid and reliable and measures six sub-constructs of the attribute of classroom engagement. Therefore, the use of such valid and reliable tools in future measurement studies of Ethiopian high school students’ mathematics and science classroom engagement is suggested.ConclusionFinally, scholars in math and science research would benefit from using this tool either to cross-validate or synthesize their studies.
Recognition and management of auditory verbal hallucinations in borderline personality disorder
Authors: Niemantsverdriet M. B. A. , Slotema C. W. , Franken I. H. A. , Brandsma J. , Verbraak M. J. P. M. , Deen M. L. , Blom J. D. , Van der Gaag M. 
Published: February 2025

BackgroundAuditory verbal hallucinations (AVH) are experienced by 25% of all people diagnosed with a borderline personality disorder. Since the impact of these hallucinations is often substantial, we asked ourselves how often they are discussed in clinical practice, whether they are treated, and if so, how and to what effect.MethodsWe studied the clinical files of 130 people under treatment at a specialized outpatient clinic for people with personality disorders, and compared the data on the presence and treatment of AVH to those collected in four prior scientific studies by our group.ResultsOf the 130 participants to those earlier studies, 42 had reported on experiencing AVH ≥ once per week. In 31% of the cases this had been missed by the treating healthcare professionals. Of the people suffering from severe AVH, a concurrent schizophrenia spectrum disorder was diagnosed in only 10%. A minority of 41% had received targeted treatment for their AVH. Doses of antipsychotics had been relatively low compared to those recommended for psychotic disorders, and yet all patients treated had reported a decrease in the frequency or severity (or both) of their AVH. Unfortunately, 93% also reported side effects, which led 57% of the group to discontinue this type of treatment. Nonpharmacological treatments such as cognitive behavioral therapy had been offered only to a minority of the participants.ConclusionEven in specialized clinical settings it remains challenging to establish the possible presence of AVH in people with a borderline personality disorder. And yet this is worth the effort because of the impact that these hallucinations tend to have, and the positive effects of antipsychotic treatment here presented. Further research is needed to develop algorithms to optimize doses in individual patients, to develop treatment guidelines, and to explore the effects of nonpharmacological treatments.
A systematic review of recovery high schools and collegiate recovery programs for building recovery capital among adolescents and emerging adults
Authors: Hennessy Emily A. , George Shane S. , Klein Morgan R. , O’Connor Jenny B. , Eddie David , Kelly John F. 
Published: February 2025

IntroductionEducational settings represent a prime opportunity for reaching adolescents and young adults (ages 12–27) experiencing substance use disorders (SUD). Recovery high schools (RHSs) were established to help adolescents in recovery finish their education while maintaining alcohol and other drug (AOD) abstinence and collegiate recovery programs (CRPs) were implemented for college and university students. With the continued proliferation of these programs, this review synthesizes the empirical literature on the impact of both types of educational recovery supports for youth.MethodThis review's methodology was based on a previous Campbell review on the same topic from 2017. We searched five public databases (May 2024), used PRISMA 2020 reporting guidelines, and followed Campbell Collaboration guidelines including Synthesis Without Meta-Analysis. Eligible studies focused on adolescent/emerging adult participants in RHSs or CRPs. Any outcome broadly related to SUD recovery was eligible. We included quantitative or mixed methods studies and allowed for cost-benefit/cost-effectiveness designs. Data from eligible studies were extracted in duplicate and assessed for quality (Quality Assessment Tool for Quantitative Studies) by four review team members. Two cost-benefit/cost-effectiveness studies included in the CRP review were rated for quality using a separate tool appropriate for these designs by a single reviewer.ResultsWe identified 37 manuscripts representing 25 unique studies focused on RHS (K = 7: N = 14,209) or CRPs (K = 18: N = 2,795). In the RHS studies, participants were predominantly White (41%) and females represented 29% of the sample. No studies met the criteria for low risk of bias. In the CRP studies, participants were predominately white (89%) and had slightly less female than male participants (45%). 11 of the 16 studies that did not use cost-benefit analysis were rated as high risk of bias. The quality rating of the two studies using cost-benefit designs indicated that both studies had fairly rigorous approaches.DiscussionThis research base suggests that students who participate in RHSs and CRPs may demonstrate reductions in AOD use, and improvements in social and academic outcomes, although given the existing research designs, statements about the incremental public health utility of investing in these programs relative to other approaches of equal intensity or duration cannot be made with confidence.
A study on the therapeutic effect of precise clipping of intracranial aneurysms assisted by CTA and 3D-slicer software
Authors: He Chuan , Cao Gang , Yang Ying , Zhong Qi , Lei Yongsheng , Tan Xingshi , Lin Xiaohong , Chen Yaokun , Sun Wenxiao 
Published: February 2025

ObjectiveTo evaluate the application of Computed Tomography Angiography (CTA) combined with 3D-Slicer software reconstruction technology in the diagnosis and preoperative planning of intracranial aneurysms, and to explore its positive significance in improving surgical outcomes for patients.MethodsFrom January 2021 to December 2023, a total of 24 patients diagnosed with intracranial aneurysms (25 cases) underwent craniotomy clipping surgery. We utilized 3D-Slicer to render and fuse preoperative CTA image data, and to formulate individualized surgical plans, including approach, bone window, anticipated protection of the aneurysm-bearing artery and perforating arteries, and compared these with the actual surgical procedures. The actual intraoperative conditions were used as the diagnostic reference standard to compare and analyze the morphology of intracranial aneurysms, assessing the consistency between preoperative planning and actual operations. Additionally, intraoperative and postoperative complications and prognosis were analyzed.ResultsIt was confirmed that among the 25 intracranial aneurysms, the combination of CTA and 3D-Slicer could effectively detect and reconstruct these aneurysms; quantifying the extent of bone window grinding could reduce the need for multiple bone grinding after opening the dura mater; the consistency in determining the stenosis of the aneurysm-bearing artery intraoperatively, the difficulty of reconstructing and separating the aneurysm neck, and the condition of the perforating arteries near the aneurysm was good, with Kappa values of 0.865, 0.779, and 0.635, respectively. However, the consistency in predicting the rupture orientation of the aneurysm was poor, with a Kappa value of 0.186. All aneurysms in this group were completely clipped, and no new signs of bleeding were found in the head CT within 24 h after surgery, and no signs of aneurysm recurrence were observed in the head CTA within 7 days.ConclusionIn summary, combining 3D-Slicer technology with CTA can accurately assess intracranial aneurysms and provide key anatomical information required for craniotomy clipping surgery to formulate surgical plans, which has a positive significance in reducing surgical complications. These findings not only lay the foundation for further exploration of related issues but also provide clinical doctors with more scientifically effective guidance for diagnosis and surgical planning.