bluuebunny/crossref_metadata_2025_split huggingface dataset:
Legume and Sesame Oral Food Challenge Outcomes
Authors: Pozin Jacob J. , Devonshire Ashley L. , Tom Kevin , Makhija Melanie , Singh Anne Marie 
Published: November 2020

Abstract

Background: Legume and sesame are emerging food allergens. The utility of specific IgE testing (sIgE) to predict clinical reactivity to these allergens is not well described.Objective: To describe clinical outcomes and sIgE in sesame and legume OFCs. Methods: We performed a retrospective review of 74 legume and sesame oral food challenges (OFC) performed between 2007-2017 at the Ann & Robert H. Lurie Children’s Hospital of Chicago. Clinical data, OFC outcome, and sIgE to legume and sesame were collected. Receiver operating characteristic curves (ROC) and logistic regression models predicting OFC outcome were generated. Results: Twenty-eight patients (median age 6.15 years) passed legume OFC (84.85%), and twenty-five patients (median age 5.91 years) passed sesame OFC (60.98%). The median sIgE to legume was 1.41 kUa/L, and 2.34 kUa/L, to sesame. For patients who failed legume OFC, 67% had cutaneous, 16.5% had gastrointestinal, and 16.5% had anaphylaxis. Of these reactions, 80% were controlled with Benadryl alone and 20% required epinephrine. For patients who failed sesame OFC, 53% had cutaneous, 12% had gastrointestinal, and 35% had anaphylaxis. Of these reactions, 6% required epinephrine, 31% were controlled with Benadryl alone, and 63% required additional epinephrine or steroids. Conclusion: Most OFC to legumes were passed and reactions to failed legume OFCs were more likely to be non-severe. Failing an OFC to sesame was almost twice as likely compared to failing a legume OFC, and reactions to failed sesame OFC were often more severe. Sesame sIgE did not correlate with OFC outcome.
A Global SWOT Analysis of Advanced Practice Nursing: Policy, Regulation, and Practice
Authors: Ladd Elissa , Miller MInna , Wheeler Kathy , Wainaina Samuel , Aguirre Francisca , McGrath Heather , Lee Susanna , Nashwan Abdulqadir , Neary Anna , Core Katherine 
Published: November 2020

Abstract
Background: The advanced practice nursing role has been characterized as task sharing between physicians and nurses, and commonly is a part of national or health system schemes to expand healthcare access and/or to improve efficiencies. However, lack of regulatory uniformity can lead to inconsistent qualifications, competencies, and a limitation of expansion of healthcare services, based on regulated scope of practice. Methods: The SWOT analysis technique (strengths, weaknesses, opportunities, threats) was used to assess the current state of advanced practice nursing worldwide, with specific focus on regulation, education, licensure, and practice. Data were collected broadly from peer reviewed, governmental and regulatory sources, as well as grey literature. Results: Key regulatory elements that frame advanced practice nursing vary significantly by country and region and remain dynamic. However, practice and educational models are outpacing regulatory standards in all six WHO regions. Overall, advanced practice nursing is expanding at an accelerated pace in order to meet population and health system needs, despite weaknesses of the regulatory system, or threats from systems that are not ready to accept innovative strategies. Conclusion: Advanced practice nursing is an innovative role that is progressing broadly in response to the global drivers of an aging population, workforce shortages, the increasing prevalence of non-communicable disease, and the need to develop system-based efficiencies for cost containment. The role is developing organically, driven by local needs, but still lags in terms of the formal regulatory legitimization in many countries. Notably, it is being buoyed by the global movement toward universal health coverage.
Elucidating the Gut Microbiome of Colorectal Cancer: 40 Fecal Bacteria as Non-invasive Biomarkers
Authors: Yuan Biao , Ma SiPing , Meng Qingkai , Du Tao , Zhu Yueyan , Yu Jing , Liang Qinsi , Wang Peicheng , Sun Zikui , Song Chun 
Published: November 2020

Abstract
Background: Colorectal adenocarcinoma (CRC) ranks one of the 5 most lethal malignant tumors both in China and worldwide. Emerging evidences have revealed the importance of gut microbiome on CRC, thus microbial community could be termed as a potential screen for early diagnosis. Importantly, compared with the whole microbial community analysis, few numbers of bacteria genus as non-invasive biomarkers with high sensitivity and specificity causing less cost would benefit more in clinical. Methods: Here we analyzed the gut microbiome from 226 CRC patients and 156 healthy people by 16s rRNA sequencing. We analyzed the microbiome diversity between CRC patients and healthy controls. We used ExtraTrees classifier to screening the biomarkers and took SVM (Support Vector Machine) model to test the specificity and sensitivity of our biomarkers. Results: Compared with the healthy gut, the microbial composition are divergent in CRC, especially the increase of some bacteria related to CRC and the decrease of some healthy bacteria. 40 bacteria genus exhibiting high weight for the healthy and CRC microbiome classification were screened as biomarkers for CRC. In addition, the combination of 40 biomarkers and FOBT showed an outstanding sensitivity and specificity for discrimination CRC patients from healthy controls. Conclusion: The method could be used as a non-invasive method for CRC early diagnosis.
A Case Report of Phelan-McDermid Syndrome: Successful Treatment with Growth Hormone Therapy
Authors: Xie RuiJin , Li TianXiao , SUN CHENYU , CE CHENG , Hua Xu , Zhao JinLin , Liu YueYing 
Published: November 2020

Abstract
Background: Phelan-McDermid syndrome (PMS), also known as 22q13.3 deletion syndrome, is a rare neurodevelopmental syndrome resulting from a deletion of the distal long arm of chromosome 22. Case presentation: We report a case of a 21 months old Chinese girl presenting with global developmental delay, absence of speech, unable to understand a few words or walk independently, insomnia, and autism-like behaviors. Second-generation sequencing and copy number variation analysis showed a heterozygous loss of SHANK3 gene in the 22q13 region, consistent with a diagnosis of PMS. After treatment with recombinant human growth hormone (rhGH), this patient had an improvement in motor skills and social behaviors. No side effects from rhGH therapy were reported. Conclusions: This is the first report of using rhGH to treat a Chinese girl diagnosed with PMS. We speculate rhGH could be a reasonable alternative choice for PMS treatment with similar clinical outcomes in comparison to insulin-like growth factor-1(IGF-1). However, further clinical trials are needed to confirm this hypothesis.
Benchmarking Total Hip Replacement Constructs Using Noninferiority Analysis: The New Zealand Joint Registry Study
Authors: Wyatt Michael , Frampton Chris , Whitehouse Michael , Deere Kevin , Sayers Adrian , Kieser David 
Published: November 2020

Abstract
Background: The aim of this study was to compare the relative performance of total hip replacement constructs and discern if there is substantial variability in performance in currently commonly used prostheses in the New Zealand Joint Registry (NZJR) using a noninferiority analysis.Methods: All patients who underwent a primary total hip replacement (THR) registered in the NZJR between 1st January 1999 to June 2020 were identified. Using a noninferiority analysis, the performance of hip prostheses were compared with the best performing contemporary construct. Construct failure was estimated using the 1-Kaplan Meier survival function method to estimate net failure. The difference in failure between the contemporary benchmark and other constructs was examined.Results: In total 135 432 THR were recorded comprising 1 035 different THR constructs. Notably 328 constructs were used just once. 48 constructs (62 251 THR) had >500 procedures at risk at 3 years post-primary of which 28 were inferior by at least 20% relative risk of which, 10 were inferior by at least 100% relative risk. 16 constructs were identified with >500 procedures at risk at 10 years with 9 inferior by at least 20%, of which one was inferior by >100% relative risk. There were fewer constructs noninferior to the best practice benchmark when we performed analysis by gender. In females at 10 years, from 5 constructs with >500 constructs at risk, 2 were inferior at the 20% margin. In males at 10 years, there were only 2 eligible constructs of which one was inferior at the 20% margin.Conclusions: We discerned that there is substantial variability in construct performance and at most time points, just over half of constructs are inferior to the best performing construct by at least 20%. These results can facilitate informed decision-making when considering THR surgery.
miR-181d/RBP2/NF-κB p65 Feedback Loop Promotes Chronic Myeloid Leukemia Blast Crisis
Authors: Zhou Minran , Yin Xiaolin , Zheng Lixin , Fu Yue , Wang Yue , Cui Zelong , Gao Zhenxing , Wang Xiaoming , Huang Tao , Jia Jihui , Chen Chunyan 
Published: November 2020

Abstract
Background: Chronic myeloid leukemia (CML) is a malignant clonal proliferative disease. Once it progresses into the phase of blast crisis (CML-BP), the curative effect is poor, and the fatality rate is extremely high. Therefore, it is becoming urgent to explore the molecular mechanisms of blast crisis transition and develop new therapeutic targets.Methods: The expression levels of miR-181d, RBP2 and NF-κB p65 were assessed in 42 newly diagnosed CML-CP patients and 15 CML-BP patients. Quantitative real-time PCR, Western blots, and cell proliferation assay were used to characterize the changes induced by overexpression or inhibition of miR-181d or RBP2 or p65. Luciferase reporter assay and CHIP assay was conducted to establish functional association between miR-181d, RBP2 and p65. Inhibition of miR-181d expression and its consequences in tumor growth was demonstrated in vivo models.Results: We found that the non-coding RNA miR-181d is overexpressed in CML-BP, which promotes leukemia cell proliferation. We identified histone demethylase RBP2 as directly downregulated by miR-181d and found that RBP2 inhibited p65 expression in leukemia cells by its binding to the p65 promoter and demethylating the tri/dimethylated H3K4 region in the p65 promoter locus. Conversely, p65 directly binds to miR-181d promoter and upregulates its expression; thus, forming a positive feedback loop.Conclusions: Taken together, the miR-181d/RBP2/p65 loop promotes CML blast transformation.
Serum Apolipoprotein B/apolipoprotein A1 Ratio in Relation to Intervertebral Disc Herniation : A Cross-sectional Frequency-Matched Case-Control Study
Authors: Chen Fei , Wu Tongde , Guo Song , Huang Junwen , Fu Qiang , pan Yaqing , Zhang Huiying , Wu Desheng , Chen Qi , Li lijun , Li Xinhua 
Published: November 2020

Abstract
BackgroundIn recent decades, the serum lipid profile of apolipoprotein(a) (Lp(a)) level and apolipoprotein B/apolipoprotein A1 ratio (Apo B/ApoA1) ratio were found more representative for serum lipid level and were recognized as the independent risk factors for various diseases. Although the serum lipid levels of total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C) were found associated with symptomatic IDH, no studies have been conducted to date for the evaluation of the association of Apo AI, Apo B, Lp(a) and Apo B/Apo AI levels with symptomatic IDH.MaterialsA total of 1,839 Chinese patients were recruited in the present study. 918 patients were diagnosed as IDH cases and were enrolled in the experimental group. A control group of 921 patients underwent a physical examination during the same period. The serum lipid levels of TC, TG, LDL-C, HDL-C, Lp(a), Apo B and Apo B/Apo AI were examined and analyzed.ResultsThe patients in the control group were collected randomly from patients who were matched with the baseline levels of the aforementioned lipid molecular. The patients with IDH exhibited significantly higher TC, TG, LDL, Apo B and Lp(a) levels compared with the control subjects. The percentage of high-TC, high-TG, high-LDL, high-Apo B and high-Lp(a) were significantly higher in the IDH group. However, hyperlipidaemia was not associated with the degenerated segment of the IDH (P=0.201). The odds ratios (OR) for the incidence of IDH with an elevated LDL-C, TC, TG, Lp(a), Apo B and Apo B/Apo AI were 1.583, 1.74, 1.62, 1.58, 1.49 and 1.39, respectively. The correlation analysis revealed the correlation between elevated LDL-C, TC, TG, Apo B, Lp(a) and incidence of IDH was significant (R2LDL=0.017; R2TC=0.004; R2TG=0.015; R2Apo B=0.004; R2LP(a)=0.021) (P<0.05). Conclusions﻿The present study suggests that elevated levels of serum TC, TG, LDL, Apo B, Lp(a) and Apo B/Apo AI are associated with a higher risk for IDH.
Impact of the COVID-19 pandemic on the Maternal Mortality Ratio: a time series study in Bahia, Brazil.
Authors: de Carvalho-Sauer Rita de Cássia Oliveira , Costa Maria da Conceição Nascimento , Teixeira Maria Gloria , Nascimento Estela Maria Ramos do , Silva Ediane Maria Filardi , Barbosa Mariana Luiza Almeida , Silva Géssica Rodrigues da , Santos Thaissa Piedade , Paixao Enny S. 
Published: November 2020

Abstract
BackgroundLittle is known about the effects of the COVID-19 pandemic on maternal mortality in Brazil. Therefore the objective of this study is to assess the impact of the COVID-19 pandemic on the maternal mortality in the State of Bahia, Brazil.MethodsThis study used state databases for data on maternal deaths and live births in Bahia, Brazil, from January 1, 2011 to September 30, 2020. The time trend of MMR was analyzed through polynomial regression analysis, of order 6. Predict monthly (Jan-Sep) and annual values,​​for 2020, were estimated by the additive Holt-Winters exponential smoothing algorithm, with 95% confidence interval, and the accuracy MMR forecasts for 2020 was assessed by checking the smoothing coefficients and the mean errors.ResultsBetween January 1 and September 30, 2020, the MMR in Bahia, Brazil, was 79.51/100,000 live births, 60.7% higher than the expected ratio (49.48 [95% CI 60.27-38.70]). The increase on maternal mortality ratio relative to expected values was observed throughout the 2020 months. However, only from May, when the COVID-19 epidemic rose sharply, it exceeded the upper limit of the 95% CI of the monthly prediction. In August, we can observe a dramatical decrease in the maternal mortality ratio, followed by an increase in September. Of the 109 registered maternal deaths in 2020, 16 (14.7%) had COVID-19 related to the causes of death.ConclusionsThe COVID-19 pandemic is causing devastating consequences for maternal mortality, in Bahia, Brazil. An urgent public health action is needed to prevent and reduce maternal deaths during this pandemic, in Brazil.
Identification of key genes and pathways in the hPSC-derived lungs infected by the SARS-CoV-2
Authors: Gu Hanming , Yuan Gongsheng 
Published: November 2020

Abstract
Coronavirus disease 2019 (COVID-19) is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which has led to numerous infections and deaths in the world. Our research is to explore the differentially expressed genes (DEGs) and signaling pathways in hPSC-derived lungs by using a bioinformatics method to clarify their potential pathogenesis. The gene expression profile of GSE155241 dataset was originally created by using an Illumina NovaSeq 6000 (Homo sapiens) platform. Functional categories and significant pathways were identified by the KEGG and GO analysis. The results suggested that brain disorders and mitochondrial dysfunctions are the main signaling pathways affected by the SARS-CoV-2 infection. Furthermore, key genes e.g. CDC20, NCBP1 and inhibitors e.g. MEK1-2-inhibitor, tivozanib may paly critical roles in COVID-19. Therefore, our study provides insights into the treatment of COVID-19 and related disorders.
Socioeconomic Difference of Husband's Involvement in Antenatal Care in Rural Indonesia
Authors: Laksono Agung Dwi , Wulandari Ratna Dwi , Kumalasari Mei Lina Fitri , Mustika Ika , Matahari Ratu 
Published: November 2020

Abstract
Background: To reduce maternal mortality, the Indonesian government campaigned for a health promotion program based on the family approach, namely “Suami Siaga” (Husband Alert). Study aims to analyze the effect of socioeconomic on the husband's involvement in ANC in rural Indonesia.Methods: The study employed data from the 2017 IDHS. Samples of 7,156 respondents were obtained. Besides the socioeconomic, other variables analyzed in this study were age, education, occupation, and parity. The final stage was employed binary logistic regression.Results: Families with the poorer socioeconomic status, the possibility of husbands being involved in ANC was 2.154 times compared to the poorest. Families with the middle socioeconomic status were more likely to have their husbands involved in ANC 2.785 times compared to the poorest. Families with a richer socioeconomic status were more likely to have their husbands involved in ANC 2.901 times compared to the poorest. Meanwhile, families with poorer socioeconomic status, the possibility of their husbands being involved in ANC was 5.299 times compared to the poorest. The results of this analysis inform that all socioeconomic statuses have a higher likelihood of husband involvement in ANC than the poorest families. The better the socioeconomic, the higher the possibility of the husband to be involved in ANC. Apart from socioeconomic, 3 other variables were also found as determinants of the husband's involvement in ANC in rural Indonesia, namely education, occupation, and parity.Conclusions: The socioeconomic status was a determinant of the husband's involvement in ANC in rural Indonesia.
Thymosin β10 promotes tumor-associated macrophages M2 conversion and proliferation via the PI3K/Akt pathway in lung adenocarcinoma
Authors: Zeng Jun , Yang Xianggui , Yang Li , Li Wancheng , Zheng Yaxin 
Published: November 2020

Abstract
Background: Thymosin β10 (TMSB10) has been reported to play a protumorigenic role in a majority of solid cancers. However, the existence of TMSB10 in immune microenvironment may contribute to the pathogenesis of lung adenocarcinoma has not been previously explored. Method: TAMs-associated TMSB10 expression was evaluated by immunohistochemistry (IHC) in 184 lung adenocarcinomas. Xenograft mice model was established to investigate the effect of TMSB10 shRNA on TAMs phenotypes. The macrophages phenotype associated cytokines IL-6, IL-8, IL-12 and TNF-α were detected by ELISA after treated with TMSB10 shRNA or scramble. Furthermore, the target proteins were detected by immunoblot. Results: We found that high TAMs-associated TMSB10 expression was significantly correlated with the advanced TNM stage and T3/T4 tumor size. And high TAMs-associated TMSB10 expression was significantly correlated with poor overall and progression-free survival of lung adenocarcinoma, acting as an independent prognostic factor for lung adenocarcinoma. Furthermore, we investigated the biological functions of TMSB10 in macrophages in vivo and in vitro. TMSB10 knockdown dramatically reduced TAMs, THP-1 and RAW264.7 cell proliferation, and promoted macrophages phenotype conversion of M2 to M1, and TMSB10 knockdown reduced the levels of p-Akt (Sec473), p-mTOR (Sec2448) and p-p70S6K (Thr389) without effect on Akt, mTOR and p70S6K expression. Conclusions: These results demonstrate that TAMs-associated TMSB10 promotes tumor growth through increasing TAMs M2 conversion and proliferation via PI3K/Akt signaling pathway, providing a promising tumor biomarker for predicting prognosis and a potential therapeutic target for lung adenocarcinoma.
The impact of COL1A1 and COL6A1 expression on hypospadias and penile curvature severity
Authors: yuri prahara , Gunadi  , Lestari Rahmadani Puji , Fardilla Firly Putri , Setyaningsih Wiwit Ananda Wahyu , Arfian Nur , Dachlan Ishandono 
Published: November 2020

Abstract
Background: Hypospadias, the most frequent congenital male external genitalia abnormality, is usually associated with curvature of the ventral penis, i.e. chordee. Abnormality of darto tissue has been suggested as the pathophysiology of chordees. Collagen is one of the most abundant fibrous proteins within the extracellular matrix. In this study, we determined the expression of collagen 1 (COL1A1) and COL6A1 in patients with hypospadias and associated them with the severity of penile curvature.Methods: We included 60 children <18 years old, consisting of 20 distal hypospadias, 20 proximal hypospadias patients, and 20 controls in our institution from 2017 – 2020. The expression of COL1A1 and COL6A1 in darto tissue was determined by reverse-transcriptase polymerase chain reaction (qPCR). The penile curvature severity was classified as mild (<30 degrees), moderate (30-60 degrees), and severe (>60 degrees).Results: qPCR showed that COL1A1 and COL6A1 expression was significantly downregulated in the distal (0.88 (0.38-2.53) and 0.54 (0.16-4.35), respectively) and proximal 0.76 (0.33-2.57) and 0.57 (0.18-1.38), respectively) hypospadias groups compared to controls (1.85 (0.24-4.61) and 0.93 (0.17-4.06), respectively) with p-values of 0.024 and 0.018, respectively. Furthermore, there was a moderate correlation between COL1A1 and COL6A1 expression (r=0.458, p<0.0001). Interestingly, COL1A1 and COL6A1 were also significantly downregulated in the moderate and severe chordee groups compared to the mild chordee groups, with p-values of 0.003 and 0.037, respectively.Conclusions: Aberrant COL1A1 and COL6A1 expression might affect abnormalities in darto tissue and penile curvature severity in hypospadias patients.
The efficacy of mesenchymal stromal cell-derived therapies for acute respiratory distress syndrome—a meta-analysis of preclinical trials.
Authors: Wang Fengyun , Fang Bin , Qiang Xinhua , Shao Jingsong , Zhou Lixin 
Published: November 2020

Abstract
Background: The investigation of mesenchymal stromal cell (MSC)-conditioned medium or extracellular vesicles (exosomes or microvesicles) as a remedy for acute lung injury (ALI) or acute respiratory distress syndrome (ARDS) has become a fast-growing field in recent years. Our purpose was to conduct a meta-analysis to investigate the efficacy of MSC-derived therapies (MDTs) for ALI/ARDS in animal models.Methods: A meta-analysis of MDTs for ALI/ARDS in animal trials was performed. PubMed and EMBASE were searched to screen relevant preclinical trials with a predetermined search strategy.Results: A total of 17 studies that compared MDT with the ALI control group were included in our study. The pooled result derived from the comparison of the two groups suggested that MDT could significantly reduce the lung injury score (standardized mean difference (SMD) = -4.02, 95% CI [- 5.28, -2.23], P < 0.0001) and improve animal survival (OR = -6.45, 95% CI [2.78, 14.97], P <0.0001). MDT mitigated the infiltration of neutrophils in alveoli (SMD = -3.38, 95% CI [-4.58, -2.18], P < 0.00001). MDT also reduced the wet-dry weight ratio of the lung (SMD = -2.34, 95% CI [-3.42, -1.26], P< 0.0001) and the total protein in BALF (SMD = -2.23, 95% CI [-3.07, -1.40], P < 0.00001). Furthermore, MDT was found to downregulate proinflammatory mediators such as IL-1, IL-6 and TNF-a and to upregulate anti-inflammatory mediators such as IL-10.Conclusion: MDT reduces lung injury and improves survival in animal ARDS models since it can ameliorate lung permeability, decrease inflammatory cell infiltration, downregulate proinflammatory mediators, and upregulate anti-inflammatory mediators. However, more animal studies and human trials are needed for further investigation.