bluuebunny/crossref_metadata_2025_split huggingface dataset:
PSI-31 Retrospective analysis of growth performance and carcass ultrasound data of yearling bulls enrolled at the Cal Poly Bull Test
Authors: Field Catherine E , Chavez Maria K , Abo-Ismail Mohammed , McFarlane Zach D 
Published: November 2020

Abstract
Since 1956, the Cal Poly Bull Test has evaluated performance of yearling bulls in a multi-breed contemporary group in the western United States. The main objectives of this study were to evaluate the consistency of the relationship between growth and ultrasound traits and between the number of bulls selling and sale price over a 19-year period from 2001–2019. Out of 3,712 bulls, a total of 2,503 Angus and 453 Hereford bulls were used in the analyses using SAS. Bulls were sold in the following pre-determined breed categories; Angus Heifer Bulls, Multi-Trait Angus Bulls, Growth Angus Bulls, and Hereford Bulls. The breed × year interaction significantly (P &lt; 0.01) affected sale price, scrotal circumference (SC), ultrasound ribeye area (UREA), ultrasound intramuscular fat percentage (UIMF), end weight, and average daily gain (ADG). Overall, Hereford bulls had significantly (P &lt; 0.0001) lower ADG than Angus bulls. A positive relationship between ADG and UREA (P &lt; 0.0001; r2 = 0.10) and between UREA and SC (P &lt; 0.0001; r2 = 0.05) was observed. A significant, positive relationship (P &lt; 0.01; r2 = 0.24) between end weight and REA was also exhibited. A significant breed × year interaction (P &lt; 0.01) was observed for sale price, wherein Angus heifer bulls consistently sold for the highest price. Additionally, sale price analyses indicated a moderate, negative relationship (P = 0.02; r2 = -0.584) between number of animals entered in the sale and price. Consistently, Angus bulls had higher (P &lt; 0.0001) sale prices. Sales with higher bull numbers were those with the lowest average prices. The year and breed category explained 31 and 22.5 % of the variance in the price, respectively. Growth performance and sale price may have a biological or genetic component as indicated by breed differences consistently over the 19-year period.
PSI-37 Post-weaning feed efficiency did not influence female fertility traits of commercial or Lowline Angus heifers
Authors: Najera Jonathan , Swanson Rebecca , Warner Andrea L , DeAtley Kasey , Doyle Stephen 
Published: November 2020

Abstract
Feed costs represent ~50% of beef production costs making selection for improved efficiency without affecting female reproduction critical to the cow/calf sector. Objectives were to determine the relationship between post-weaning feed efficiency and female fertility in commercial (n = 19) and half-blood Lowline-influenced Angus heifers (n = 20). Yearling heifers were delivered to the CSU, Chico Farm cattle feeding unit 45-d post weaning and randomly assigned to one of three 7 x 18 m pens, equipped with GrowSafe feeding units. Heifers were given ad libitum access to water and a forage-based diet targeting 0.68 kg gain/d (TDN: 54%, CP: 14%, and NDF: 39.7%, on a DM basis) for 72-d. Feed efficiency measures included dry matter intake (DMI), feed conversion ratio (FCR) and residual feed intake (RFI). Heifer RFI was calculated as the difference between predicted DMI and actual DMI during the trial. Feed efficiency groups were identified as: 1) low RFI (0.5 SD above the RFI mean), 2) marginal RFI (+/- 0.5 SD about the RFI mean), and 3) high RFI (0.5 SD above the RFI mean). Serial weights were taken every 14-d to calculate average daily gain (ADG) and metabolic midweight (MMWT). Heifers were synchronized using a 14-d CIDR-PG protocol. Heifers received PG injection 16-d post-CIDR removal and were equipped with an estrous detection patch. Estrous behavior was observed morning and evening for 90-h. Heifers were artificially inseminated (AI) 12-h post-standing estrus and injected with 2 mL GnRH. Pregnancy determination via rectal palpation occurred 6-mos after initial AI. Reproductive measures included: first service conception (FSC), pregnancy rate (PR), and response to estrous synchronization (RES). Reproductive traits were analyzed using the GLM procedures of SPSS with a binary logistic response. Breed type and feed efficiency group were independent variables. Relationships among reproductive measures and performance were analyzed using logistic regression of SPSS, fitting the independent variables of ADG, DMI, and RFI. Traits FSC, PR, and RES did not differ among breed types or feed efficiency groups (P &gt; 0.05) and DMI, ADG and RFI were not predictors of FSC, PR, nor RES (P &gt; 0.05). Cumulatively, results indicate there is no relationship between feed efficiency and reproductive performance. Producers can select for low RFI to decrease feed costs without compromising reproductive performance.
Post-marketing surveillance study of trifluridine/tipiracil in patients with metastatic colorectal cancer
Authors: Yoshino Takayuki , Uetake Hiroyuki , Funato Yuya , Yamaguchi Yasunobu , Koyama Takahiko , Ozawa Daisuke , Tajiri Masaru , Muro Kei 
Published: November 2020

Abstract

Background
The novel oral nucleoside antineoplastic agent trifluridine/tipiracil was approved for metastatic colorectal cancer in Japan in March 2014. In this post-marketing surveillance study, we investigated the safety and efficacy of trifluridine/tipiracil in a real-world setting, particularly haematological drug reactions classified according to the baseline renal and hepatic functions.

Methods
We investigated patients with metastatic colorectal cancer who received trifluridine/tipiracil during the first four treatment cycles prospectively. The patients typically received 35 mg/m2 trifluridine/tipiracil twice daily on days 1–5 and 8–12 every 28 days. The primary objective was to assess the safety of trifluridine/tipiracil, but its efficacy was also evaluated.

Results
Between July 2014 and June 2016, 860 patients were enrolled in the study, and the safety and efficacy of trifluridine/tipiracil were evaluated in 823 patients. Adverse drug reactions occurred in 89.7% of the patients. The most common adverse drug reactions were decreased white blood cell count (67.0%) and neutrophil count (63.9%). Haematological drug reactions of grade ≥3 were observed in 41.7% of the patients with normal renal function; 50.3, 65.6 and 78.9% of the patients had mild, moderate and severe renal impairments, respectively. Hepatic impairment was not associated with a higher incidence of haematological drug reactions. The median overall survival was 8.4 months, with a 1-year survival rate of 33.7%.

Conclusion
This post-marketing surveillance study further confirmed the safety and tolerability profile of trifluridine/tipiracil observed in a clinical study setting.
Exposure to Tobacco Smoke and Temperament among U.S. Children 0–5 Years Old
Authors: Merianos Ashley L , Nabors Laura A , Fiser Kayleigh A , Mahabee-Gittens E Melinda 
Published: November 2020

Abstract

Objectives
This study examined the association between tobacco smoke exposure (TSE) and temperament among children 0–5 years old overall and within age groups: 0–2 and 3–5 years.

Methods
Data were obtained from the 2017–2018 NSCH (N = 14,345). TSE status was defined as whether children lived with a smoker who: does not smoke inside the home (no home TSE) or smokes inside the home (home TSE). We conducted logistic regression analyses while controlling for covariates.

Results
Overall, 12.5% of children lived with a smoker with no home TSE and 1.1% had home TSE. Children with home TSE were at increased odds to not always: be affectionate and tender (aOR = 1.74, 95% CI = 1.18–2.58), show interest and curiosity (aOR = 1.81, 95% CI = 1.23–2.68), and smile and laugh (aOR = 1.77, 95% CI = 1.13–2.77) than those with no TSE. Among 0- to 2-year-olds, those with home TSE were more likely to not always be affectionate and tender (aOR = 1.97, 95% CI = 1.04–3.74). Among 3- to 5-year-olds, those who lived with a smoker with no home TSE were more likely to not always: bounce back quickly (aOR = 1.21, 95% CI = 1.05–1.40) and smile and laugh (aOR = 1.26, 95% CI = 1.03–1.54), and those with home TSE were more likely to not always: show interest and curiosity (aOR = 2.24, 95% CI = 1.40–3.59) and smile and laugh (aOR = 2.43, 95% CI = 1.43–4.11).

Conclusions
Tobacco smoke-exposed children were at increased odds of not always demonstrating positive early childhood temperament behaviors, with 3- to 5-year-olds having more pronounced odds.
Outpatient Fluoroquinolone Use in Children, 2000–2018
Authors: Ross Rachael K , Gerber Jeffrey S , Willis Zachary I , Hersh Adam L , Kinlaw Alan C 
Published: November 2020

AbstractBackgroundWhile fluoroquinolones are commonly used in adults, the use in children has been low. Since 2000, there were 3 US Food and Drug Administration (FDA) Boxed warnings regarding fluoroquinolones (2008, 2013, and 2016). Our objective was to describe the use of fluoroquinolones in children and assess the impact of 3 recent FDA warnings on fluoroquinolone use.MethodsFrom 2000 to 2018, we assessed claims for all outpatient prescription fills to measure the use of systemic fluoroquinolones and other broad-spectrum antibiotics in children  less than 18 years old in the MarketScan Commercial Claims and Encounters database. We describe demographics, indication for antibiotic, and clinical characteristics. To assess the impact of FDA warnings on fill rates, we conducted an interrupted time-series analysis.ResultsThe cohort included 34.6 million unique beneficiaries less than 18 years old with 441 062 fluoroquinolone fills (5.5 fills per 1000 person-years). The fluoroquinolone fill rate was highest among children &gt; 11 years old. Urinary tract infection was the most common associated diagnosis (21.8%). Since 2008, the fluoroquinolone fill rate has declined. By the end of the study period in December 2018, in the (counterfactual) absence of the FDA warnings, fluoroquinolone fill rate would have been 7.5 (95% confidence interval [CI]: 5.2-9.7); however, the corresponding rate in observed data was 2.8 (95% CI: 1.7-3.9).ConclusionsFluoroquinolone use was low compared with other common broad-spectrum antibiotics and declining trends over time were associated with FDA warnings, even though these warnings were not pediatric specific. Future work should assess the adverse events at issue in these warnings in children.
Knocking out NEGATIVE REGULATOR OF PHOTOSYNTHESIS 1 increases rice leaf photosynthesis and biomass production in the field
Authors: Chen Faming , Zheng Guangyong , Qu Mingnan , Wang Yanjie , Lyu Ming-Ju Amy , Zhu Xin-Guang 
Published: November 2020

Abstract
Improving photosynthesis is a major approach to increasing crop yield potential. Here we identify a transcription factor as a negative regulator of photosynthesis, which can be manipulated to increase rice photosynthesis and plant biomass in the field. This transcription factor, named negative regulator of photosynthesis 1 (NRP1; Os07g0471900), was identified through a co-expression analysis using rice leaf RNA sequencing data. NRP1 expression showed significantly negative correlation with the expression of many genes involved in photosynthesis. Knocking out NRP1 led to greater photosynthesis and increased biomass in the field, while overexpression of NRP1 decreased photosynthesis and biomass. Transcriptomic data analysis shows that NRP1 can negatively regulate the expression of photosynthetic genes. Protein transactivation experiments show that NRP1 is a transcription activator, implying that NRP1 may indirectly regulate photosynthetic gene expression through an unknown regulator. This study shows that combination of bioinformatics analysis with transgenic testing can be used to identify new regulators to improve photosynthetic efficiency in crops.
The CIB1 transcription factor regulates light- and heat-inducible cell elongation via a two-step HLH/bHLH system
Authors: Ikeda Miho , Mitsuda Nobutaka , Ishizuka Toru , Satoh Mai , Ohme-Takagi Masaru 
Published: November 2020

Abstract
Light and high temperature promote plant cell elongation. PHYTOCHROME INTERACTING FACTOR4 (PIF4, a typical basic helix-loop-helix [bHLH] transcriptional activator) and the non-DNA binding atypical HLH inhibitors PHYTOCHROME RAPIDLY REGULATED1 (PAR1) and LONG HYPOCOTYL IN FAR-RED 1 (HFR1) competitively regulate cell elongation in response to light conditions and high temperature. However, the underlying mechanisms have not been fully clarified. Here, we show that in Arabidopsis thaliana, the bHLH transcription factor CRYPTOCHROME-INTERACTING BASIC HELIX-LOOP-HELIX 1 (CIB1) positively regulates cell elongation under the control of PIF4, PAR1, and HFR1. Furthermore, PIF4 directly regulates CIB1 expression by interacting with its promoter, and PAR1 and HFR1 interfere with PIF4 binding to the CIB1 promoter. CIB1 activates genes that function in cell elongation, and PAR1 interferes with the DNA binding activity of CIB1, thus suppressing cell elongation. Hence, two antagonistic HLH/bHLH systems, the PIF4–PAR1/HFR1 and CIB1–PAR1 systems, regulate cell elongation in response to light and high temperature. We thus demonstrate the important role of non-DNA binding small HLH proteins in the transcriptional regulation of cell elongation in plants.
TCP5 controls leaf margin development by regulating KNOX and BEL-like transcription factors in Arabidopsis
Authors: Yu Hongyang , Zhang Ling , Wang Weiyao , Tian Peng , Wang Wei , Wang Keyi , Gao Zhong , Liu Shuai , Zhang Yongxia , Irish Vivian F , Huang Tengbo 
Published: November 2020

Abstract
Development of leaf margins is an important process in leaf morphogenesis. CIN-clade TCP (TEOSINTE BRANCHED1/CYCLOIDEA/PCF) transcription factors are known to have redundant roles in specifying leaf margins, but the specific mechanisms through which individual TCP genes function remain elusive. In this study, we report that the CIN-TCP gene TCP5 is involved in repressing the initiation and outgrowth of leaf serrations by activating two key regulators of margin development, the Class II KNOX factor KNAT3 and BEL-like SAW1. Specifically, TCP5 directly promotes the transcription of KNAT3 and indirectly activates the expression of SAW1. We also show that TCP5 regulates KNAT3 and SAW1 in a temporal- and spatial- specific manner that is largely in accordance with the progress of formation of serrations. This regulation might serve as a key mechanism in patterning margin morphogenesis and in sculpting the final form of the leaf.
Pseudomonas putida MPE, a manganese-dependent endonuclease of the binuclear metallophosphoesterase superfamily, incises single-strand DNA in two orientations to yield a mixture of 3′-PO4 and 3′-OH termini
Authors: Ghosh Shreya , Ejaz Anam , Repeta Lucas , Shuman Stewart 
Published: November 2020

Abstract
Pseudomonas putida MPE exemplifies a novel clade of manganese-dependent single-strand DNA endonuclease within the binuclear metallophosphoesterase superfamily. MPE is encoded within a widely conserved DNA repair operon. Via structure-guided mutagenesis, we identify His113 and His81 as essential for DNA nuclease activity, albeit inessential for hydrolysis of bis-p-nitrophenylphosphate. We propose that His113 contacts the scissile phosphodiester and serves as a general acid catalyst to expel the OH leaving group of the product strand. We find that MPE cleaves the 3′ and 5′ single-strands of tailed duplex DNAs and that MPE can sense and incise duplexes at sites of short mismatch bulges and opposite a nick. We show that MPE is an ambidextrous phosphodiesterase capable of hydrolyzing the ssDNA backbone in either orientation to generate a mixture of 3′-OH and 3′-PO4 cleavage products. The directionality of phosphodiester hydrolysis is dictated by the orientation of the water nucleophile vis-à-vis the OH leaving group, which must be near apical for the reaction to proceed. We propose that the MPE active site and metal-bound water nucleophile are invariant and the enzyme can bind the ssDNA productively in opposite orientations.
Exposure to Nicotine and Toxicants Among Dual Users of Tobacco Cigarettes and E-Cigarettes: Population Assessment of Tobacco and Health (PATH) Study, 2013–2014
Authors: Smith Danielle M , Christensen Carol , van Bemmel Dana , Borek Nicolette , Ambrose Bridget , Erives Gladys , Niaura Raymond , Edwards Kathryn C , Stanton Cassandra A , Blount Benjamin C , Wang Lanqing , Feng Jun , Jarrett Jeffery M , Ward Cynthia D , Hatsukami Dorothy , Hecht Stephen S , Kimmel Heather L , Travers Mark , Hyland Andrew , Goniewicz Maciej L 
Published: November 2020

Abstract

Introduction
Concurrent use of tobacco cigarettes and e-cigarettes (“dual use”) is common among tobacco users. Little is known about differences in demographics and toxicant exposure among subsets of dual users.

Aims and Methods
We analyzed data from adult dual users (current every/some day users of tobacco cigarettes and e-cigarettes, n = 792) included in the PATH Study Wave 1 (2013–2014) and provided urine samples. Samples were analyzed for biomarkers of exposure to nicotine and selected toxicants (tobacco-specific nitrosamine NNK [NNAL], lead, cadmium, naphthalene [2-naphthol], pyrene [1-hydroxypyrene], acrylonitrile [CYMA], acrolein [CEMA], and acrylamide [AAMA]). Subsets of dual users were compared on demographic, behavioral, and biomarker measures to exclusive cigarette smokers (n = 2411) and exclusive e-cigarette users (n = 247).

Results
Most dual users were predominant cigarette smokers (70%), followed by daily dual users (13%), non-daily concurrent dual users (10%), and predominant vapers (7%). Dual users who smoked daily showed significantly higher biomarker concentrations compared with those who did not smoke daily. Patterns of e-cigarette use had little effect on toxicant exposure. Dual users with high toxicant exposure were generally older, female, and smoked more cigarettes per day. Dual users who had low levels of biomarkers of exposure were generally younger, male, and smoked non-daily.

Conclusions
In 2013–2014, most dual users smoked cigarettes daily and used e-cigarettes occasionally. Cigarette smoking appears to be the primary driver of toxicant exposure among dual users, with little-to-no effect of e-cigarette use on biomarker levels. Results reinforce the need for dual users to stop smoking tobacco cigarettes to reduce toxicant exposure.

Implications
With considerable dual use of tobacco cigarettes and e-cigarettes in the United States, it is important to understand differences in toxicant exposure among subsets of dual users, and how these differences align with user demographics. Findings suggest most dual users smoke daily and use e-cigarettes intermittently. Low exposure to toxicants was most common among younger users, males, and intermittent smokers; high exposure to toxicants was most common among older users, females, and heavier cigarette smokers. Results underscore the heterogeneity occurring within dual users, and the need to quit smoking cigarettes completely in order to reduce toxicant exposure.
Some new maxims of chancery: ‘Explicated for the more facile introduction of such as are studiously addicted to that noble profession’
Authors: Qayyum Sham 
Published: November 2020

Abstract
Over the past half century, the trust has undergone momentous change. A generation of Chancery practitioners reduced it from being a doctrine to a loophole. What, perhaps, many did not realise was that the changes would mostly be for the worse. Before explaining which aspects of the modern express trust trouble my conscience, I divide its history into Three Ages of the Trust. We now live in the Age of the Loophole Trust (1969–). To help us understand this latest stage, I utilise equity’s most venerable teaching aid – the maxim. My remarks are structured around three new (surreptitious) maxims.
Compartment Syndrome in Children With a Supracondylar Fracture: Not Everyone has Risk Factors
Authors: Armstrong Douglas G. , MacNeille Rhett , Lehman Erik B. , Hennrikus William L. 
Published: November 2020

Objectives:
To clarify the incidence, associated conditions, and timing of fasciotomy for compartment syndrome (CS) in children with a supracondylar (SC) fracture of the humerus.

Design:
A retrospective trauma system database study.

Setting:
Accredited trauma centers in Pennsylvania.

Patients:
A statewide trauma database was searched for children 2–13 years of age admitted with a SC fracture between January 2001 and December 2015. Four thousand three hundred eight children met inclusion criteria.

Intervention:
Treatment of a SC fracture.

Main Outcome Measurement:
Diagnosis of CS/performance of a fasciotomy.

Results:
During the study period, 21 (0.49%) children admitted with a SC fracture of the humerus were treated with fasciotomy. CS/fasciotomy was more likely in males (P = 0.031), those with a nerve injury (P = 0.049), and/or ipsilateral forearm fracture (P < 0.001). Vascular procedure, performed in 18 (0.42%) children, was strongly associated with CS/fasciotomy (P < 0.001). Closed reduction and fixation of a forearm fracture was associated with CS (P = 0.007). Timing of SC fracture treatment did not influence outcome. Fasciotomy was performed subsequent to reduction in 13 subjects; mean interval between procedures was 23.4 hours (r = 4.5–51.3).

Conclusions:
Risk factors for CS exist; however, they are not required for the condition to develop. CS may develop subsequent to admission and/or SC fracture treatment. In timing of operative management and hospitalization, the results support contemporary practice.

Level of Evidence:
Prognostic Level III. See Instructions for Authors for a complete description of levels of evidence.
Prevalence and Risk Factors of Problematic Internet Use and Its Relationships to the Self-Esteem and Health-Related Quality of Life
Authors: Cam Hasan Huseyin , Ustuner Top Fadime 
Published: November 2020

Abstract

Purpose
This study aimed to investigate the prevalence of problematic internet use (PIU) among high school students, to evaluate its potential risk factors, and to investigate its relation to self-esteem and health-related quality of life.

Methods
This cross-sectional study included 1,558 high school students in Turkey. Participants completed the Internet Addiction Test as well as the Rosenberg Self-Esteem Scale and 12-item Short Form Health Survey. Demographics and internet usage patterns were also collected. Data analysis was conducted by descriptive and analytical statistics (Pearson chi-square tests, independent-samples t test, and binary logistic regression).

Results
The mean age of the participants was 16.20 ± 1.05 years. Nearly 21.1% of the participants exhibited PIU (Internet Addiction Test score ≥ 50). The findings indicated that PIU was associated with poor self-esteem and poor physical and mental health. The PIU was significantly higher among students using the internet for social networking. The chi-square analysis showed a significant correlation between the severities of PIU and the students' type of school, residence, perceived academic performance, and relationship with parents (p < .05). No significant correlation was found between PIU severity and age group, gender, number of siblings, paternal or maternal educational levels, self-reported family income, sleep duration, or whether or not they resided with parents (p > .05).

Conclusions
PIU was found to be a common problem among high school students, and it was significantly correlated with self-esteem and health-related quality of life. To alleviate this public health problem, at-risk groups should be identified and timely management strategies and effective prevention programs should be established.