bluuebunny/crossref_metadata_2025_split huggingface dataset:
hsa_circ_0000231 promotes colorectal cancer cell growth through upregulation of CCND2 by IGF2BP3/miR-375 dual pathway&nbsp;
Authors: Zhang Wei , Wang Bo , Yang Yang , Zhang Zhen , Wang Quan , Zhang Haoran , Jiang Kewei , Ye Yingjiang , Wang Shan , Shen Zhanlong 
Published: August 2020

Abstract
Background and aim Circular RNAs (circRNAs) have emerged as vital regulators of the initiation and progression of diverse kinds of human cancers. This study aimed to investigate the role of circRNAs in colorectal cancer (CRC). Methods The expression profiles of circRNAs in five pairs of CRC tissues and adjacent normal tissues were analyzed using microarray. Quantitative real-time polymerase chain reaction, in situ hybridization, and BaseScope Assay were used to determine the level and prognostic values of hsa_circ_0000231. Then, in vitro and in vivo functional experiments were performed to investigate the effects of hsa_circ_0000231 on cell proliferation. Mechanistically, fluorescence in situ hybridization, dual-luciferase reporter assay, and RNA pull-down and RNA immunoprecipitation experiments were performed to confirm the interaction between hsa_circ_0000231 and Insulin-like growth factor 2 mRNA-binding protein 3(IGF2BP3) or has_miR-375. Results The expression of hsa_circ_0000231 was upregulated in CRC primary tissues, which indicated poor prognosis of patients with CRC. The results demonstrated that hsa_circ_0000231 could promote CRC cell proliferation as well as tumorigenesis in vitro and in vivo. The mechanistic analysis showed that hsa_circ_0000231 might, on the one hand, act as a competing endogenous RNA of miR-375 to promote cyclin D2 (CCND2) and, on the other hand, bind to the IGF2BP3 protein to prevent CCND2 degradation. Conclusion The findings suggested that hsa_circ_0000231 facilitated CRC progression by sponging miR-375 or binding to IGF2BP3 to modulate CCND2, implying that has_circ_0000231 might be a potential new diagnostic and therapeutic biomarker of CRC.
Effects of Tocilizumab in COVID-19 patients: a cohort study
Authors: Vu Christine A. , Deronde Kailynn J , Vega Ana D , Maxam Meshell , Holt Gregory , Natori Yoichiro , Zamora Jose Gonzales , Salazar Veronica , Boatwright Renata , Morris Stephen R , Corvino Daniela de Lima , Betances Anmary Fernandez , Colucci Leah , keegan James , Lopez Andy , Rezk Andrew Hany , Rodriguez Yvette , Doblecki Susanne , Zerda David De La , Abbo Lilian M 
Published: August 2020

Abstract
Background
Due to the lack of proven therapies, we evaluated the effects of early administration of tocilizumab for COVID-19. By inhibition of the IL-6 receptor, it has been proposed that tocilizumab may help to mitigate the hyperinflammatory response associated with progressive respiratory failure.
Methods
A retrospective, observational study was conducted on hospitalized adults who received intravenous tocilizumab for COVID-19 between March 23, 2020 and April 10, 2020.
Results
Most patients were male (66.7%), Hispanic (63.3%) or Black (23.3%), with a median age of 54 years. Tocilizumab was administered at a median of 8 days (range 1–21) after initial symptoms and 2 days (range 0–12) after hospital admission. On the day of administration, the median PaO2/FiO2 was 166 (range 33–523) and 50 patients (83.3%) had ARDS. By day 30, 36 patients (60.0%) demonstrated clinical improvement, 9 (15.0%) died, 33 (55.0%) were discharged alive, and 18 (30.0%) remained hospitalized. Successful extubation occurred in 13 out of 29 patients (44.8%). Infectious complications occurred in 16 patients (26.7%) at a median of 10.5 days. There was an increase in PaO2/FiO2 and an initial reduction in CRP that was not sustained beyond day 10.
Conclusions
Majority of patients demonstrated clinical improvement and were successfully discharged from the hospital alive after receiving tocilizumab. Similar to previous studies, infectious complications were not uncommon. A rebound effect with CRP was observed, which may suggest the need for higher or subsequent doses to adequately manage cytokine storm. Based on our findings, we believe that tocilizumab may have a role in the treatment of COVID-19, however randomized controlled studies are urgently needed.
Analysis of Long Noncoding RNA and mRNA Profiling in Peripheral Blood Mononuclear Cells of Human Immunized With Rabies Virus Vaccine by RNA Sequencing
Authors: Zhao Pingsen , Hou Kaijian , Zeng Peibin , Yang Songtao , Xia Xianzhu 
Published: August 2020

Abstract

Background
Rabies is still a lethal disease caused by rabies virus (RABV), and it remains a global health threaten. The epigenetic landscape of host in response to immunization with rabies virus vaccine is not yet elucidated. Evidences reveal that long noncoding RNA (lncRNA) play an important role for control of viral infection, but the expression profile of lncRNA in human immunized with rabies virus vaccine remains unclear.
Methods
lncRNA and mRNA profiles were investigated in four volunteers vaccinated with RABV vaccine by RNA sequencing.
Results
33 lnRNAs and 427 mRNAs were differentially expressed in RABV vaccine immunized volunteers. The gene oncology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were used to analysis the functional annotation, and the result suggested the lncRNAs involved in signaling pathways related to host immune induced by the RABV vaccine.
Conclusions
To the best of our knowledge, it is the first time to report the transcriptomic landscape of lncRNAs in human immunized with RABV vaccine. Our study mimics the host response of human to RABV infection and suggests that lncRNAs might be of a potential therapeutic target in anti-rabies virus strategies.
Evaluating Quality of Life in Families With Williams Syndrome Patients
Authors: MORALEDA ESTHER , Arana Mario , López Patricia 
Published: August 2020

Abstract
Background: Williams Syndrome is a developmental disorder characterized by a variable intellectual disability. People with Williams Syndrome need the intervention of several clinical and educational specialists throughout their life. However, little is known about the impact produced by this disability in their immediate environment, especially in families. The purpose was to know the level of quality of life described by families with Williams Syndrome.Methods: The sample was made up of 33 families belong to Spanish Williams Syndrome Association who were evaluated using the Kidslife Scale. Theirs children and adolescents were between 4 and 20 years old. Eight main quality of life dimensions were evaluated: emotional well-being, physical well-being, material well-being, personal development, interpersonal relations, social inclusion, self-determination and rightsResults: The obtained data indicated that the degree and presence of intellectual disability themselves did not homogeneously influence people’s quality of life, but many variables could alter their quality of life to a greater or lesser extent. There are no significant differences between quality of life areas but significant differences appeared for level of dependence in the self-determination subarea (p<.05). Conclusions: These results led us to analyse the social and emotional implications for families and their environment.
Characteristics of human papillomavirus infection among women with cervical cytological abnormalities in the Zhoupu District, Shanghai City, China, 2014–2019
Authors: Li Ping , Liu Qing , Li Wei , Liu Zhou , Xing Baoling , Wu Suqin , Zhou Zhaoli , Sun Liping , Ren He , Li Hengfeng , Li Huaping 
Published: August 2020

Abstract
Background: Human papillomavirus (HPV) infection is currently the main cause of cervical cancer and precancerous lesions in female patients. By analyzing 6-year patient data from Shanghai Zhoupu Hospital in China, we retrospectively analyzed the epidemiological characteristics of women to determine the relationship between HPV genotype and cytological test results.Methods: From 2014 to 2019, 23,724 cases of cervical shedding were collected from Zhoupu Hospital in Shanghai, China. By comparing the results of HPV and ThinPrep cytology test (TCT), the HPV infection rate of patients was retrospectively analyzed. HPV genotyping using commercial kits can detect 21 HPV subtypes (15 high-risk and 6 low-risk). According to the definition of the Bethesda system, seven types of cervical cytology results are involved.Results: 3,816 among 23,724 women, nearly 16.08%, were infected with HPV. The top three highest HPV prevalence rates were high-risk type infection, including HPV52 (3.19%), 58 (2.47%) and 16 (2.34%). The number of single-type HPV infections (3,480 (91.20%)) was much larger than the number of multi-type ones (336 (8.8%)). Single-type infections were mainly in women aged 50-60 (16.63%) and women under 30 (15.37%), while multi-type infections were more common in women over 60 (2.67%). By analyzing the long-term trends, between 2014 and 2019, HPV52, 58, and 16 subtypes changed significantly, and the HPV positive rate also changed significantly during this period.Among 4,502 TCT positive women, 15 (4.04%), 125 (2.64%) ,159 (1.54%), 4,202(17.71%) and 1(0.004%) had atypical squamous cells (ASC), high-grade squamous intraepithelial lesions (HSIL), low-grade squamous intraepithelial lesions (LSIL), atypical glandular cells (AGC) and cervical adenocarcinoma, respectively. The HPV infection rates were 66.08%, 63.99%, 115.20%, 119.50%, and 31.72% for NILM, AGCs, HSILs LSILs and ASCs, respectively.Conclusions: HPV and TCT screening were very important steps in the secondary prevention of cervical cancer. Through the tracking and analysis of HPV and TCT results in this study, it can provide valuable information for Shanghai's HPV screening and prevention strategies, and provide references for clinical decision-making in the treatment of cervical cancer and precancerous lesions.
A Head-To-Head Comparative Study of Covid-19 Patients Between Epicenter and Peripheral Areas of Pandemic From China
Authors: Wang Gang , Luo Feng Min , Liu Dan , Liu Jia Shen , Wang Ye , Chen Hong , Tian Pan Wen , Fan Tao , Tang Li , Yu He , Wang Lan , Feng Mei , Ni Zhong , Wang Bo , Song Zhi Fang , Wu Xiao Lin , Wang Hong Jun , Tong Xiang , Xue Miao , Lei Xian Ying , Long Bo , Jia Chao , Xiao Jun , Shang Juan , Xiong Nian , Luo Jian Fei , Liang Zong An , Li Wei Min 
Published: August 2020

Abstract
Background: There has been little information on difference of epidemiology, clinical characteristics and outcomes between epicenter and peripheral areas of Covid-19 pandemic. Methods: Based on Sichuan & Wuhan collaboration research group for Covid-19, we established two retrospective cohorts reflecting the epicenter and peripheral area of pandemic. Epidemiology, clinical characteristics and outcomes of patients were compared. Multivariate regression analyses were used to estimate adjusted odds ratios (aOR) to identify clinical variables associated with outcomes.Results: Upon March 12, 2020, Wuhan cohort consisted of 710 patients using random sampling, and 474 consecutive cases constituted Sichuan cohort. Sichuan cohort had more upper airway symptoms, while Wuhan cohort is elder, has more lower airway symptoms and comorbidities. Wuhan cohort had higher risk of death (aOR=7.64), ICU admission (aOR=1.66), delayed time from illness onset to hospital and ICU admission (aOR=6.29 and aOR=8.03) and prolonged duration of viral shedding (aOR=1.64). Conclusions: Worse outcomes in the epicenter would be explained by delayed time from illness onset to hospital and ICU admission associated with elevated systemic inflammation reflecting organ dysfunction and prolonged duration of virus shedding except for age and comorbidities. It indicates potentially clinical implications of Covid-19 that early supportive care would achieve better clinical outcome.
Association of Cardio-Metabolic Risk Factors and Basic and Instrumental Activities of Daily Living Among Elderly People;Bushehr Elderly Health Program
Authors: Khalagi Kazem , Ansarifar Akram , Fahimfar Noushin , Sanjari Mahnaz , Gharibzdeh Safoora , Sharifi Farshad , Shafiee Gita , Heshmat Ramin , Nabipour Iraj , Larijani Bagher , Ostovar Afshin 
Published: August 2020

Abstract
Background: Iran's population is aging. One of the most common problems in Iranian public health is the high prevalence of cardio-metabolic risk factors. The purpose of this study was to investigate the relationship between cardio-metabolic and demographic risk factors and disability in people 60 years and older in Iran.Methods: Data from the Bushehr Elderly Health (BEH) program was used in the analysis. Demographic characteristics, as well as the history of diabetes and other chronic diseases, and smoking were measured using standardized questionnaires. Anthropometric measurements and laboratory tests were performed under standard conditions. Dependency was determined by the questionnaires of basic activities of daily living (BADL) and instrumental activities of daily living (IADL) using Barthel and Lawton scales respectively. Multiple logistic regression was used in the analysis.Results: Mean (Standard Deviation) of the participants’ age was 69.34 (6.4) years (range: 60 and 96 years), and 48.06% of the participants were men. After adjusting for potential confounders, being older, being female (OR (95%CI): 2.33 (1.89 - 2.86)), having a lower education level, a history of diabetes mellitus (OR: 1.43 (1.17 -1.74)) and past smoking (OR: 1.25 (1.00 - 1.55)), and no physical activity (OR: 1.54 (1.23 - 1.87)) were significantly associated with dependency in IADL. Also, being older and female (OR: 2.38 (1.85 - 3.03)), having a lower education level, no physical activity (OR: 2.17 (1.61 - 2.86)) and low daily intake of calories were associated with dependency in BADL.Conclusion: Age, gender, education level and physical activity are risk factors of the dependency in both of BADL and IADL in the elderly people. Diabetes mellitus and past smoking are risk factors of the dependency in IADL, and low HDL cholesterol and low daily intake of calories are risk factors of the dependency in BADL.
A Novel Anatomic Titanium Mesh Cage for Reducing the Subsidence Rate After Anterior Cervical Corpectomy: A Finite Element Study
Authors: Wang Yuhang , Zhao Qinpeng , Zhang Yongyuan , Zhang Haiping , He Baorong , Yan Liang , Hao Dingjun , Wang Biao 
Published: August 2020

Abstract
Background: Fusion with a titanium mesh cage (TMC) has become popular as a conventional method after cervical anterior corpectomy, but postoperative TMC subsidence has often been reported in the literature. We designed a novel anatomic cervical TMC to reduce the postoperative subsidence rate. This finite element study aims to investigate the anti-subsidence performance of a novel anatomic TMC and provide a theoretical basis for clinical application. Methods: According to the test process specified in the ASTM F2267 standard, three-dimensional finite element analysis was used to compare the anti-subsidence characteristics of a traditional TMC (TTMC) and novel TMC (NTMC). Abaqus software was used for the mesh, and the modulus of elasticity, Poisson's ratio and other material coefficients of each part were input into the model. Solidworks software was used to establish the TTMC and NTMC models and construct the two three-dimensional finite element models of TMC subsidence testing mentioned above. Through analysis, the relationships between the stiffness of the intervertebral body fusion device (Kd) and the stiffness of the polyurethane foam blocks (Ks) of the TTMC and NTMC were derived, respectively. Results: After fitting the linear segment, the Kd values of the TTMC and NTMC were 37314 N/mm and 89124 N/mm, respectively. After calculation, the Ks values of the TTMC and NTMC were 653.83 N/mm and 995.95 N/mm, respectively. The Kp values of the TTMC and NTMC were calculated to be 665.5 N/mm and 1007.2 N/mm according to the formula. Conclusion: The reported novel anatomic titanium mesh cage (NTMC) significantly improved the anti-subsidence performance after ACCF, which was approximately 51.3% higher than that of the traditional titanium mesh cage.
Cancer Incidence and Mortality in the U.S. Astronaut Corps, 1959-2017
Authors: Reynolds Robert , Little Mark P , Day Steven M , Charvat Jacqueline , Blattnig Steve , Huff Janice L , Patel Zarana S 
Published: August 2020

Abstract
Background: Cancer incidence and mortality are important outcomes in the surveillance of long-term astronaut health. In this research, we compare cancer incidence rates, cancer-specific mortality rates, and cancer case fatality ratios in US astronauts with those in the US general population.Methods: We use standardized incidence ratios and standardized mortality ratios to index the incidence and mortality of various cancers against rates in the US general population, from the US astronaut cohort inception in April 1959 through 31 December 2017. We also compare the lethality of these cancers in astronauts and the general population using the relative case fatality ratio.Results: The astronaut cohort included 338 individuals and over 9600 person-years of follow-up time. The counts of most cancers were under 3, though there were 11 cases of melanoma and 30 cases of prostate cancer. Both prostate and melanoma had statistically significant increases in incidence, though only melanoma had a significant increase in mortality. Lung cancer had a statistically significant deficit of both cases and deaths, while colon cancer had sizable (but not statistically significant) reductions in incidence and mortality. Three cancers showed evidence of detection bias (colon, hematologic, prostate), possibly a result of astronaut health screening protocols. For all cancers combined, astronauts showed a non-significant reduction in incidence and mortality, and a significant reduction in case fatality ratio.Conclusions: Though there were observed increases in both incidence and mortality from melanoma among astronauts, these increases are consistent with those observed repeatedly among aircraft pilots, suggesting this may be associated with ultraviolet radiation or lifestyle factors rather than any astronaut-specific exposure. The increase in prostate cancer incidence is likely explained by detection bias, and the same may be true for hematologic cancers. The lack of statistical significance in the reduction of incidence and mortality for colon cancer may be attributable to relaxed screening practices for astronauts in recent years. As astronaut health surveillance continues and evolves, the growing database will lead to a clearer picture over time. The methods employed here provide a useful structure for ongoing analysis of this unique occupational cohort.
Fibroblast Growth Factor 21 Predicts and Promotes Vascular Calcification in Haemodialysis Patients
Authors: Jiang Liqiong , Yin Qing , Yang Min , Li Min , Pan Mingming , Han Yucheng , Zhao Zhen , Wang Zhi , Zhu Lili , Wei Qing , Tu Yan , Gao Min , Liu Hong , Zhang Xiaoliang , Liu Bi-cheng , Wang Bin 
Published: August 2020

Abstract
Background: Cardiovascular disease (CVD) is the leading cause of death in haemodialysis (HD) patients. Vascular calcification (VC) is dramatically accelerated and is strongly associated with CVD events and mortality in HD patients. VC coexists with osteoporosis in many studies. Fibroblast growth factor 21 (FGF21) as an adipocytokines is a new hypoglycemic strategy and is inversely related to bone mineral density.Methods: To evaluate the contribution of FGF21 to VC in HD patients, we preliminary sreened 802 HD patients of two large HD centers in China. At last 388 HD patients were entered this cross-sectional study. We detected circulating FGF21 levels and measured the whole thoracic aorta calcification scores (TACS) and calcification scores of the three segments of thoracic aorta (TA), including ascending thoracic aorta (ATACS), aortic arch (AoACS), and descending thoracic aorta (DTACS) of our 388 HD patients. In addition, we pre-incubated human aortic endothelial cells (HAECs) with FGF21 in the presence or absence of parathyroid hormone (PTH) in vitro.Results: The median serum FGF21 level in HD patients was 11-fold higher than that in healthy controls. Ln(FGF21) was positively correlated with Ln(TACS+1), Ln(ATACS+1), Ln(AoACS+1) and Ln(DTACS+1) respectively in HD patients. Serum FGF21 was independently associated with TACS and ATACS, AoACS, and DTACS. FGF21 combined with age, calcium and intact parathyroid hormone demonstrated a high area under the curve (AUC=0.84) with optimal sensitivity (84%) and specificity (71%) for the prediction of VC in HD patients. Our vitro results showed that FGF21 enhanced the calcification effect of PTH on HAECs by increasing calcium deposition and endothelial-to-mesenchymal transition (EndMT).Conclusions: Circulating FGF21 was notably higher and was a potential predictor and promoter of VC in HD patients.Trial registration Chinese Clinical Trial Registry, identifier: ChiCTR1900028249. Registered 16 December 2019-Retrospectively registered,http://www.medresman.org.cn/uc/project/projectedit.aspx?proj=5981
Evolution of Medical Students' Perception of the Patient's Right to Privacy
Authors: Terán Alberto Álvarez , Palazuelos Camilo , Dierssen-Sotos Trinidad , Llorca Javier , Gómez-Acebo Inés 
Published: August 2020

Abstract
Background: During clinical rotations, medical students experience situations in which the patients’ right to privacy may be violated. The aim of this study is to analyze medical students' perception of clinical situations that affect patients' right to privacy or intimacy, and to look for the influential factors that may contribute to the infringement on their rights, such as age, gender, academic year or parents’ educational level.Methods: A cross-sectional study was conducted with a survey via “Google Drive”. It consisted of 16 questions about personal information, 24 questions about their experience when rotating and 22 questions about their opinion concerning several situations related to the right to privacy. Results: 129 medical students from various Spanish medical schools participated. Only 31% of 3rd-6th year students declared to have signed a confidentiality agreement when starting their clinical practice, and most students (52%) reported that doctors “sometimes”, “rarely” or “never” introduce themselves and the students when entering the patients’ rooms. Additionally, about 50% of all students reported that they would take a picture of a patient’s hospitalization report without his/her (consent), which would be useful for an assignment. Conclusions: Important mistakes during medical students’ rotations have been observed, as well as general lack of knowledge regarding patient’s right to privacy among Spanish medical students. Men and older students showed better knowledge of current legislation, as well as those whose parents were both college-educated and those in higher academic years. Informative courses and law teaching should be enhanced so that patients’ right to privacy may be better guaranteed.