bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sequential Associations Between Communication Acts of Children With and Without Autism Spectrum Disorder and Maternal Verbal Responses
Authors: Ashtari Atieh , Yadegari Fariba , Samadi Sayyed Ali , Watson Linda R. 
Published: September 2020

In this study, the sequential associations between child communication acts, including spontaneous communication (SC) and elicited communication (EC), and the types of verbal responses of Iranian mothers (follow‐in nondirective, follow‐in directive, and redirective responses) were compared between children with autism spectrum disorder (ASD) and young typically developing (TD) children. Participants were 29 children with ASD aged 3–6 years and 40 TD children aged 13–18 months, matched on expressive vocabulary. Using time‐window sequential analysis, maternal verbal responses within a time interval of 3 sec following child communication were examined during 15 min of video‐recorded mother–child free play interaction. Mothers in the two groups had broadly similar patterns of response to child communication acts, but some differences in responding to child EC. Across both groups, sequential associations were stronger for maternal follow‐in nondirective responses to child SC than for this type of response to child EC, and were stronger for follow‐in directive responses to child EC than for follow‐in directive responses to child SC. Child EC and SC acts were less likely to be followed by redirective responses than other maternal responses, again across both groups. Finally, mothers of children with ASD were more likely than mothers of TD children to follow‐in to child EC with both nondirective and directive responses. Our findings suggest that mothers of children with ASD synchronize their responses with their child's SC acts to the same extent as mothers of TD children, and are more synchronous in responding to their child's EC acts.Lay SummaryThis observational study examined how Iranian mothers verbally responded to their children's communication acts, based on whether the children's communication was spontaneous (unprompted) or elicited (prompted by the mother). Mothers of children with autism spectrum disorder or typical development responded to their children's spontaneous communication acts in similar ways, but showed some differences in responding to children's elicited communication. By prompting their children to communicate, mothers create opportunities to give additional verbal responses to their children, which may help to support children's further language development.
In situ product recovery techniques aiming to obtain biotechnological products: A glance to current knowledge
Authors: Santos Ariane G. , de Albuquerque Tiago L. , Ribeiro Bernardo D. , Coelho Maria Alice Z. 
Published: September 2020

AbstractBiotechnology and bioengineering techniques have been widely used in the production of biofuels, chemicals, pharmaceuticals, and food additives, being considered a “green” form of production because they use renewable and nonpolluting energy sources. On the other hand, in the traditional processes of production, the target product obtained by biotechnological routes must undergo several stages of purification, which makes these processes more expensive. In the past few years, some works have focused on processes that integrate fermentation to the recovery and purification steps necessary to obtain the final product required. This type of process is called in situ product recovery or extractive fermentation. However, there are some differences in the concepts of the techniques used in these bioprocesses. In this way, this review sought to compile relevant content on considerations and procedures that are being used in this field, such as evaporation, liquid–liquid extraction, permeation, and adsorption techniques. Also, the objective of this review was to approach the different configurations in the recent literature of the processes employed and the main bioproducts obtained, which can be used in the food, pharmaceutical, chemical, and/or fuel additives industry. We intended to elucidate concepts of these techniques, considered very recent, but which emerge as a promising alternative for the integration of bioprocesses.
Temperature‐Dependent Sex Determination in Sea Turtles in the Context of Climate Change: Uncovering the Adaptive Significance
Authors: Santidrián Tomillo Pilar , Spotila James R. 
Published: September 2020

AbstractThe adaptive significance of temperature‐dependent sex determination (TSD) in reptiles remains unknown decades after TSD was first identified in this group. Concurrently, there is growing concern about the effect that rising temperatures may have on species with TSD, potentially producing extremely biased sex ratios or offspring of only one sex. The current state‐of the‐art in TSD research on sea turtles is reviewed here and, against current paradigm, it is proposed that TSD provides an advantage under warming climates. By means of coadaptation between early survival and sex ratios, sea turtles are able to maintain populations. When offspring survival declines at high temperatures, the sex that increases future fecundity (females) is produced, increasing resilience to climate warming. TSD could have helped reptiles to survive mass extinctions in the past via this model. Flaws in research on sex determination in sea turtles are also identified and it is suggested that the development of new techniques will revolutionize the field.
Water‐soluble luminescent copper nanoclusters as a fluorescent quenching probe for the detection of rutin and quercetin based on the inner filter effect
Authors: Sasikumar Thangarasu , Ilanchelian Malaichamy 
Published: September 2020

AbstractIn the present study, we proposed a simple, sensitive and selective fluorescence method for the detection of rutin (Rut) and quercetin (Que) based on the inner filter effect (IFE) utilizing water‐ soluble cysteine‐stabilized copper nanoclusters (Cys‐CuNCs) as a fluorescent probe. The Cys‐CuNCs were successfully synthesized and characterized using UV–visible absorption, emission, Fourier‐transform infrared (FT‐IR) spectroscopy, fluorescence lifetime, high resolution transmission electron microscopy (HR‐TEM) and zeta potential measurements. Cys‐CuNCs exhibited bluish‐green luminescence under UV light with characteristic emission maxima at 486 nm. Cys‐CuNCs was successfully exploited as fluorescent probes for the detection of Rut/Que. The addition of increasing concentrations of Rut/Que led to a gradual decrease in the emission intensity of Cys‐CuNCs. The decrease in Cys‐CuNC emission intensities were attributed to the strong IFE and static quenching mechanism. The calculated limit of detection values for Rut and Que were as low as 0.021 μM and 0.035 μM, respectively. The Cys‐CuNCs sensing probe exhibited excellent selectivity in the presence of other potential interfering compounds. Furthermore, the present method was successfully applied to the analysis of both Rut and Que in biological samples.
Reconstitution of an Anti‐HER2 Antibody Paratope by Grafting Dual CDR‐Derived Peptides onto a Small Protein Scaffold
Authors: See Kyra , Kadonosono Tetsuya , Ota Yumi , Miyamoto Kotaro , Yimchuen Wanaporn , Kizaka‐Kondoh Shinae 
Published: September 2020

AbstractTarget‐binding small proteins are promising alternatives to conventional monoclonal antibodies (mAbs), offering advantages in terms of tissue penetration and manufacturing costs. Recently, a design strategy to create small proteins called fluctuation‐regulated affinity proteins (FLAPs) consisting of a structurally immobilized peptide from the complementarity‐determining region (CDR) loops of mAbs (CDR‐derived peptide) and a protein scaffold was developed. Because mAb paratopes are usually composed of multiple CDRs, FLAPs with multiple binding peptides may have an enhanced target‐binding capability. Here, a strategy to create FLAPs bearing dual CDR‐derived peptides (D‐FLAPs) using the anti‐human epithelial growth factor receptor type 2 (HER2) mAb trastuzumab as a basis is developed. Computationally selected CDR‐derived peptides are first grafted onto two adjacent loops of the fibronectin type III domain (FN3) scaffold, yielding 80 D‐FLAP candidates. After computational screening based on their similarity to the parental mAb with regard to the conformation of paratope residues, two candidates are selected. After further evaluation with ELISA, one D‐FLAP with HYTTPP and GDGFYA peptides from CDR‐L3 and CDR‐H3 of the parental mAb, respectively, is found to bind HER2 with a dissociation constant of 58 nm. This method applies to various mAb drugs and allows the rational design of small protein alternatives.
Analysis of chemotherapy effect on the second primary malignancy for head and neck cancer patients by a nomogram based on SEER database
Authors: Li Xinrong , Guo Kaibo , Feng Yuqian , Guo Yong 
Published: September 2020

AbstractSecond primary malignancy (SPM) ranks the second leading cause of death in patients with head and neck cancer (HNC), while studies exploring the risk factors for SPM are limited. To clarify this, we investigated the relationship between the chemotherapy and SPM using the Surveillance, Epidemiology, and End Results (SEER) database. 11 345 patients initially diagnosed with HNC between 1998 and 2016 were selected from the SEER database. First, these patients were divided into two groups according to chemotherapy or not. With Fine and Gray model, the subdistribution hazard ratio (sHR) of chemotherapy was calculated based on Propensity Score Matching (PSM). Second, the 11 345 cases were randomized into a training set and a validation set. Based on the training set, the different cumulative incidence of SPMs between the patients with and without chemotherapy was estimated respectively in the high‐ and low‐risk group according to the scores derived from a nomogram. Chemotherapy was negatively correlated to the SPMs (sHR: 0.847, 95% CI: 0.733‐0.977, P = .023) by conducting competing risk analysis. With chemotherapy, forest plots showed subgroups of squamous cell carcinoma (SCC, sHR: 0.815, 95% CI: 0.7‐0.948, P = .008), 50‐64 years old (sHR:0.794, 95% CI: 0.655‐0.962, P = .019), male (sHR:0.828, 95% CI: 0.703‐0.974, P = .023), and well/moderate histological grade (sHR:0.828, 95% CI: 0.688‐0.996, P = .045) were negatively correlated to SPMs; the nomogram showed the high‐risk population characterized as SCC, elder age, male, and well/moderate histological grade also tended to have lower incidence of SPMs (sHR: 0.805, 95% CI: 0.669‐0.969, P = .022). Despite HNC patients with characteristics of SCC, increased age, male, and well/moderate histological grade had higher risk of a SPM, they were also more likely to be benefitted from chemotherapy to avoid it.
Feasibility of group theraplay with refugee children in Turkey
Authors: Eruyar Seyda , Vostanis Panos 
Published: September 2020

AbstractDespite the evidence on the role of parental factors, especially attachment relationships, in mediating complex trauma experienced by refugee children, there is limited evidence on the use of attachment‐based interventions. The aim of this study was to explore the feasibility of adapting such an intervention, group theraplay, for refugee children in Turkey. Fifteen child–parent dyads participated, and completed measures on attachment relationships, child mental health and parenting strategies. Children engaged with the intervention, whilst it proved more difficult to involve parents regularly. There was a significant improvement in children's post‐traumatic stress and other common mental health symptoms, as well as parent‐rated attachment relationship difficulties. Attachment‐based modalities using play, activities and games can be particularly useful for children and families with cultural and language challenges. Therapeutic interventions for refugee children and families should be integrated with other types of support within a multimodal service approach.
Building up PtII−Thiosemicarbazone−Lysine−sC18 Conjugates
Authors: Haseloer Alexander , Lützenburg Tamara , Strache Joss Pepe , Neudörfl Jörg , Neundorf Ines , Klein Axel 
Published: September 2020

AbstractThree chiral tridentate N^N^S coordinating pyridine‐carbaldehyde (S)‐N4‐(α‐methylbenzyl)thiosemicarbazones (HTSCmB) were synthesised along with lysine‐modified derivatives. One of them was selected and covalently conjugated to the cell‐penetrating peptide sC18 by solid‐phase peptide synthesis. The HTSCmB model ligands, the HTSCLp derivatives and the peptide conjugate rapidly and quantitatively form very stable PtII chlorido complexes [Pt(TSC)Cl] when treated with K2PtCl4 in solution. The Pt(CN) derivatives were obtained from one TSCmB model complex and the peptide conjugate complex through Cl−→CN− exchange. Ligands and complexes were characterised by NMR, IR spectroscopy, HR‐ESI‐MS and single‐crystal XRD. Intriguingly, no decrease in cell viability was observed when testing the biological activity of the lysine‐tagged HdpyTSCLp, its sC18 conjugate HdpyTSCL‐sC18 or the PtCl and Pt(CN) conjugate complexes in three different cell lines. Thus, given the facile and effective preparation of such Pt‐TSC‐peptide conjugates, these systems might pave the way for future use in late‐stage labelling with Pt radionuclides and application in nuclear medicine.
Intravascular lithotripsy in saphenous vein grafts and graft stent failure: A case series
Authors: Øksnes Anja , McEntegart Margaret 
Published: September 2020

AbstractThe long‐term patency of saphenous vein grafts (SVGs) is poor compared to arterial bypass grafts. Re‐do coronary artery bypass graft surgery (CABG) is high risk, thus graft failure often requires revascularization with percutaneous coronary intervention (PCI). While PCI to the native vessels is preferable, PCI to SVGs is sometimes necessary despite being associated with a high incidence of stent failure. While early SVG degeneration is associated with friable disease, calcification becomes an increasing problem in older grafts. Intravascular Lithotripsy (IVL) has been demonstrated to be safe and effective in the treatment of calcified native coronary artery disease (CAD). We present the first case series of five patients undergoing PCI to de novo SVG disease or SVG stent failure utilizing IVL for calcium modification.
Microwave Detection of Wet Triacetone Triperoxide (TATP): Non‐Covalent Forces and Water Dynamics
Authors: Blanco Susana , Macario Alberto , García‐Calvo José , Revilla‐Cuesta Andrea , Torroba Tomas , López Juan Carlos 
Published: September 2020

AbstractThe water adducts of triacetone triperoxide (TATP) have been observed by using broadband rotational spectroscopy. This work opens a new way for the gas‐phase detection of this improvised explosive. The observed clusters exhibit unusual water dynamics and rarely observed multicenter interactions. TATP‐H2O is formed from the D3 symmetry conformer of TATP with water lying close to the C3 axis. Water rotation around this axis with a very low barrier gives rise to the rotational spectrum of a symmetric top. The main interaction of the monohydrate is a four‐center trifurcated donor Ow‐H⋅⋅⋅O hydrogen bond, not observed previously in the gas phase, reinforced by a weak four‐center trifurcated acceptor C−H⋅⋅⋅Ow interaction. Surprisingly, all structural signatures show the weakness of these interactions. The complex TATP‐(H2O)2 is formed from the monohydrated TATP by the self‐association of water.
Ba3(BO3)(CO3)F: The First Borate Carbonate Fluoride Synthesized by the High‐Temperature Solution Method
Authors: Huang Chunmei , Zhang Fangfang , Cheng Shichao , Yang Zhihua , Li Hao , Pan Shilie 
Published: September 2020

AbstractIn contrast to the well‐investigated halogen‐containing borates and carbonates, very few halogen‐containing borate carbonate compounds have been reported. Specifically, no example of borate carbonate fluoride has been synthesized successfully until now. Herein, the planar π‐conjugated units [BO3]3− and [CO3]2− and the F− ions are introduced simultaneously into one crystal structure resulting in the first borate carbonate fluoride, Ba3(BO3)(CO3)F, by the high‐temperature solution method in the atmosphere. Its structure features a hexagonal channel formed by the [BO3]3− and [CO3]2− units with the [F3Ba8]13+ trimers filled in the channel. Various characterizations including single crystal‐ and powder‐XRD, EDX, IR, UV‐vis‐NIR, and TG‐DSC, together with the first principles calculation have been carried out to verify the structure and fully understand the structure–property relationships.
Structure‐Directing Roles of Organic Molecules in the Formation of Aluminosilicate and Aluminophosphate Molecular Sieves Revealed by 2D 1H DQ‐SQ NMR Spectroscopy
Authors: Liu Fangyan , Wang Biao , Liu Xiaolong 
Published: September 2020

AbstractUnderstanding of crystallization mechanisms of molecular sieves is driven by the broad range of usefulness and unique properties they possess. It is still difficult to obtain information related to the crystallization mechanism of molecular sieves, partly because the materials are generally prepared under hydrothermal conditions and the whole reaction happens in the “black box” autoclave. In this work, 2D 1H DQ‐SQ NMR results clearly demonstrate that it is not only the electrostatic interactions between organic structure‐directing agents (OSDAs) and the framework, but also the correlation among OSDAs playing the dominant structural directing roles during the crystallization process. Our fundamental understanding of the crystallization mechanism of molecular sieves could be of great value to design and synthesize new molecular sieves with desirable structural properties.
Understanding the Uniqueness of 2p Elements in Periodic Tables
Authors: Wang Zhen‐Ling , Hu Han‐Shi , von Szentpály László , Stoll Hermann , Fritzsche Stephan , Pyykkö Pekka , Schwarz W. H. Eugen , Li Jun 
Published: September 2020

AbstractThe Periodic Table, and the unique chemical behavior of the first element in a column (group), were discovered simultaneously one and a half centuries ago. Half a century ago, this unique chemistry of the light homologs was correlated to the then available atomic orbital (AO) radii. The radially nodeless 1s, 2p, 3d, 4f valence AOs are particularly compact. The similarity of r(2s)≈r(2p) leads to pronounced sp‐hybrid bonding of the light p‐block elements, whereas the heavier p elements with n≥3 exhibit r(ns) ≪ r(np) of approximately −20 to −30 %. Herein, a comprehensive physical explanation is presented in terms of kinetic radial and angular, as well as potential nuclear‐attraction and electron‐screening effects. For hydrogen‐like atoms and all inner shells of the heavy atoms, r(2s) ≫ r(2p) by +20 to +30 %, whereas r(3s)≳r(3p)≳r(3d), since in Coulomb potentials radial motion is more radial orbital expanding than angular motion. However, the screening of nuclear attraction by inner core shells is more efficient for s than for p valence shells. The uniqueness of the 2p AO is explained by this differential shielding. Thereby, the present work paves the way for future physical explanations of the 3d, 4f, and 5g cases.
Remodeling of projections from ventral hippocampus to prefrontal cortex in Alzheimer's mice
Authors: Xu Feng , Ono Munenori , Ito Tetsufumi , Uchiumi Osamu , Wang Furong , Zhang Yu , Sun Peng , Zhang Qing , Yamaki Sachiko , Yamamoto Ryo , Kato Nobuo 
Published: September 2020

AbstractEmotional dysregulation often accompanies cognitive deficits in Alzheimer's disease (AD). The hippocampus, most notably damaged by AD pathology, is classified into the cognition‐bound posterior and emotion‐bound anterior hippocampi. Since the anterior hippocampus or its rodent counterpart, the ventral hippocampus (VH), sends dense afferents to the prefrontal cortex (PFC) and the basolateral amygdala (BLA), the two structures implicated in fear responses, we investigated whether these afferents are modified in 3xTg AD model mice. An anterograde dextrin tracer injected into VH revealed that axons in PFC were more ramified in 3xTg than wild‐type (WT) mice, with the synaptic density reduced. The VH projections to BLA were not affected. Intracellular accumulation of amyloid β (Aβ) or Aβ‐like immunoreactivity was found in PFC and BLA neurons alike. Behaviorally, in the 2‐way active avoidance test, the frequency of chamber change was higher, with the test performance better, in 3xTg than WT mice, suggesting a distorted contextual fear in the 3xTg group. Given the essential involvement of parts of PFC in contextual fear responses and that of BLA in fear responses in general, the observed remodeling of VH‐to‐PFC afferents and the accumulation of intracellular Aβ in BLA and PFC pyramidal cells might exercise critical influences on enhanced avoidance behavior in 3xTg mice.
Nitrogen‐doped Binary Spinel CuCo2O4/C Nanocomposite: An Efficient Electrocatalyst for Oxygen Evolution Reaction
Authors: Tian Jin , Wang Zhichao , Wang Yue , Yuan Ding , Tian Fenghui , Zhang Lixue 
Published: September 2020

AbstractThe oxygen evolution reaction (OER) on the anode side is one of the bottlenecks for electrochemical water splitting, and the search for cost‐effective and efficient catalysts for OER is highly pursued. Herein, through calcination and nitridation treatment of the Cu−Co based bimetallic metal‐organic framework precursor, nitrogen doped CuCo2O4/nitrogen‐doped carbon (N−CuCo2O4@N−C) are successfully synthesized. X‐ray photoelectron spectroscopy measurements reveal the evident change of electronic state after doping N atoms into CuCo2O4@N−C. When used as an OER electrocatalyst, N−CuCo2O4@N−C catalysts offer an overpotential of only 260 mV to reach 10 mA cm−2, which is comparable to commercial RuO2 catalyst. The N−CuCo2O4@N−C sample possesses a lower Tafel slope than pristine CuCo2O4@N−C sample. Moreover, a chronoamperometric study reveals that N−CuCo2O4@N−C displays excellent stability for at least 28 h. This work provides a promising heteroatom doping strategy to fabricate high‐performance OER electrocatalysts for water splitting.
Tactile colour pictogram to improve artwork appreciation of people with visual impairments
Authors: Cho Jun Dong , Quero Luis Cavazos , Bartolomé Jorge Iranzo , Lee Do Won , Oh Uran , Lee Inae 
Published: September 2020

AbstractA recent development in tactile technology enables an improvement in the appreciation of the visual arts for people with visual impairment (PVI). The tactile sense, in conjunction with, or a possibly as an alternative to, the auditory sense, would allow PVIs to approach artwork in a more self‐driven and engaging way that would be difficult to achieve with just an auditory stimulus. Tactile colour pictograms (TCPs), which are raised geometric patterns, are ideographic characters that are designed to enable PVIs to identify colours and interpret information by touch. In this article, three TCPs are introduced to code colours in the Munsell colour system. Each colour pattern consists of a basic cell size of 10 mm × 10 mm to represent the patterns consistently in terms of regular shape. Each TCP consists of basic geometric patterns that are combined to create primary, secondary, and tertiary colour pictograms of shapes indicating colour hue, intensity and lightness. Each TCP represents 29 colours including six hues; they were then further expanded to represent 53 colours. Two of them did not increase the cell size, the other increased the cell size 1.5 times for some colours, such as yellow‐orange, yellow, blue, and blue‐purple. Our proposed TCPs use a slightly larger cell size compared to most tactile patterns currently used to indicate colour, but code for more colours. With user experience and identification tests, conducted with 23 visually impaired adults, the effectiveness of the TCPs suggests that they were helpful for the participants.