bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of feces storage conditions for host-microbiota screenings in C. elegans
Authors: Caron Laury , Alonzo De la Rosa Claudia Miriam , Diop Khoudia , Miard Stéphanie , Taubert Stefan , Marette André , Picard Frédéric 
Published: December 2024

Background and aimsCurrent research on host-gut microbiota interactions is hindered by almost infinite bacterial combinations depending on intrinsic characteristics, environment, and health status, which prevents large-scale screenings in mammals. For these reasons, the bacterivore model organism C. elegans has been developed to test the effects of gut microbiota extracts from mammals. This study tested whether storage conditions of mouse feces and fecal extracts modify normal C. elegans healthspan.MethodsFeces from mice were processed for microbiota extraction after collection or after one or twelve months at -80 °C and compared to microbiota extracted six months before and left at room temperature. Extracts were probed for bacterial composition, viability, and nutritional content and tested in synchronized wild-type (strain N2) worms for food preferences and intake, development, fat accumulation, brood size, and maximal lifespan.ResultsLong-term freezing of feces before microbiota extraction modified composition but did not negatively impact subsequent worm development, fat accumulation, reproduction, and maximal lifespan, whereas using samples extracted and left at room temperature after a long period of time resulted in robust avoidance and was detrimental for normal growth.ConclusionsUsing frozen feces to test for impacts of microbiota in C. elegans appears an appropriate method since it did not affect normal biology and healthspan, which supports protocols with already existing feces stored in biobanks for high-throughput phenotype screenings.
Comparison of postoperative pain and stress using a multimodal approach in cats: open vs. laparoscopic-assisted ovariohysterectomy
Authors: Jeong Changwoo , Yi Kangwoo , Yu Yong , Heo Suyoung 
Published: December 2024

IntroductionLaparoscopic surgery is increasingly utilized for its minimally invasive nature, leading to reduced postoperative pain and faster recovery. This study aimed to compare postoperative pain, surgical complications, and recovery between laparoscopic-assisted ovariohysterectomy (LAOHE) and open ovariohysterectomy (OHE) in cats.MethodsA total of 40 healthy female cats were randomly assigned to undergo either LAOHE (n = 20) or OHE (n = 20). Pain scores were assessed using the Glasgow Composite Pain Scale at 1, 4, 8, 12, and 24 h postoperatively. Blood samples were collected to measure cortisol levels as a stress biomarker. Complications were recorded intraoperatively and postoperatively.ResultsCats in the LAOHE group exhibited significantly lower pain scores compared to the OHE group at 1, 4, and 8 h postoperatively (P &lt; 0.05). Cortisol levels were also significantly lower in the LAOHE group (P &lt; 0.05). There were no significant differences in surgical time or postoperative complications between the two groups.DiscussionThe findings suggest that LAOHE results in reduced postoperative pain and stress in cats compared to OHE, without increasing surgical time or complications. LAOHE may be a preferable technique for elective ovariohysterectomy in cats.
Transcriptome Analysis Reveals the Mechanism of Y0-C10-HSL on Biofilm Formation and Motility of Pseudomonas aeruginosa
Authors: Tang Deping , Liu Yali , Yao Huihui , Lin Yanyan , Xi Yanpeng , Li Mengjiao , Mao Aihong 
Published: December 2024

Background: Pseudomonas aeruginosa (P. aeruginosa) is a type of pathogen that takes advantage of opportunities to infect and form biofilm during infection. Inhibiting biofilm formation is a promising approach for the treatment of biofilm-related infections. Methods: Here, Y0-C10-HSL (N-cyclopentyl-n-decanamide) was designed, synthesized, and tested for its effect on biofilm formation, motility, and the Caenorhabditis elegans (C. elegans) survival assay. In addition, the molecular mechanism of Y0-C10-HSL on P. aeruginosa biofilm formation was explored using transcriptome analysis. Results: At a concentration of 200 μmol/L Y0-C10-HSL, biofilm and exopolysaccharides were decreased by 38.5% and 29.3%, respectively; Y0-C10-HSL effectively dispersed the pre-formed biofilm and inhibited the motility ability of P. aeruginosa; and the C. elegans survival assay showed that Y0-C10-HSL was safe and provided protection to C. elegans against P. aeruginosa infection (the survival rates of C. elegans were higher than 74% and increased by 39%, 35.1%, and 47.5%, respectively, when treated with 200 μmol/L Y0-C10-HSL at 24, 48, and 80 h). Transcriptome analysis showed that 585 differentially expressed genes (DEGs) were found after treatment with 200 μmol/L Y0-C10-HSL, including 254 up-regulated DEGs and 331 down-regulated DEGs. The genes involved in the quorum sensing system and biofilm formation were down-regulated. Conclusions: Y0-C10-HSL inhibited the biofilm formation and dispersed the pre-formed biofilm of P. aeruginosa through down-regulated genes related to quorum sensing pathways and biofilm formation. These findings provide a theoretical foundation for the treatment and prevention of antibiotic resistance in clinical and environmental microorganisms such as P. aeruginosa.
Interpersonal Strategies in Academic Writing: The Significance of Acknowledging Research Limitations and Recommendations for Future Studies in Scientific Discourse
Authors: Tikhonova Elena V. 
Published: December 2024

Introduction: Describing the limitations of current research and recommendations for further studies in original and review papers is a mandatory step, demonstrating critical self-assessment and the author's level of expertise. To affirm their contribution to existing knowledge in a subject area, scholars must demonstrate methodological rigor and critical understanding of the nature of knowledge in their field. Linguistic embodiment becomes fundamentally important in implementing this step.Purpose: To explore the role of interpersonal strategies in academic writing in the context of acknowledging research limitations and formulating recommendations for future studies.Materials and Methods: The study is based on the analysis of articles in the field of agricultural sciences, published over the last three years, using genre and corpus analysis methods.Results: The strategies authors of scientific papers use to discuss their research are described, demonstrating critical reflection and a commitment to transparency in presenting results. Particular attention in the article is given to various interpersonal linguistic strategies, such as the use of evaluative phrases, negation, and modal verbs, in the context of discussing research limitations and recommendations for future studies. The authors emphasize the importance of such strategies for establishing trustful relationships with readers and demonstrating scientific rigor. The article also discusses metadiscursive and textual references, which help authors present limitations and recommendations as an integral part of the academic research process.Conclusions: The study underscores the interconnection between the sections on research limitations and recommendations for future studies in academic writing, showing that the quality of one section directly influences the quality of the other. This interaction fosters the development of scientific dialogue and constructive discussion within the academic community. The article makes a significant contribution to understanding how improving one of these sections can enhance the effectiveness and significance of the other, providing valuable recommendations for researchers, especially those who use English as a second language, in their quest to improve their writing and publishing skills.
Experimental Evaluation of the Effect of Complex Treatment with High Pressure and Ultrasound on Microbiological Parameters of Liquid Egg Melange
Authors: Sokolov Sergey A. , Deyneka Innesa G. , Dekan' Aleksej A. , Yashonkov Aleksander A. 
Published: December 2024

Introduction. The intensive development of technology using high pressure is due not only to its ability to inactivate pathogenic microflora, but also to the prospects that open up for giving new useful consumer properties to food products. Also, one of the effective ways of energy influence on technological processes is the use of acoustic vibrations. Numerous previous studies confirm the prospects of these methods of processing food raw materials. However, the physical processes occurring in a liquid medium under the influence of ultrasonic vibrations, which are superimposed on other force fields (high pressure, electric fields, etc.), are insufficiently studied.Purpose. Experimental evaluation of the synergistic effect of combined high-pressure and ultrasound treatment on the example of decontamination of egg white, yolk and homogeneous egg melange.Materials and Methods. Liquid egg white, yolk and liquid homogeneous egg melange, i.e. a mixture of protein and yolk, were accepted as the object of research. The studies were carried out on an automated installation that allows conducting studies at pressures from 0.1 to 1000 MPa, at temperatures from + 5 to + 100 ° C, the frequency of ultrasonic exposure from 17 to 35 kHz; the power of ultrasonic exposure from 0 to 300 watts.Results. The study of the microbiological contamination of egg melange, in which the initial concentration of E. coli K12DH5a was approximately 108 CFU/ml, was carried out separately when it was treated with ultrasound with a power of 20, 30 and 40 watts, high pressure of 200 MPa for 400 seconds and when it was treated with ultrasound and high pressure. The analysis of the obtained results shows that the treatment of liquid egg melange with ultrasound in the selected power range does not ensure its sterilization. The high-pressure treatment of melange ensures its sterilization after 350 seconds.Conclusion. Complex treatment with high pressure in the range of 250-300 MPa and ultrasound with a power of 40 watts at a radiation frequency of 25 kHz reduces the processing time by approximately 30% with a decrease in operating pressure from 250 to 200 MPa.
Методология определения косвенных информационных признаков сложных систем
Authors: Алексеев Д.С. 
Published: December 2024

Рассматриваются новые подходы к изучению волновых явлений в объектах и процессах устройств киберфизических систем через их косвенные признаки. С точки зрения исследования информационного состояния систем дается определение понятия «косвенные признаки». Раскрыты примеры использования косвенных признаков в теории систем. Классификация косвенных признаков и моделей их изучения предлагается для решения задач обеспечения информационной безопасности систем путем контроля их косвенных признаков.
New approaches to the study of wave phenomena in objects and processes of devices of cyberphysical systems through their indirect signs are considered. From the point of view of the study of the information state of systems the definition of the concept "indirect signs" is given. Examples of using indirect signs in the theory of systems are disclosed. Classification of indirect signs and models of their study are proposed for solving problems of information security of systems by controlling their indirect signs.
The Salford Nature Environments Database (SNED): an open-access database of standardized high-quality pictures from natural environments
Authors: Bendall Robert C. A. , Royle Sam , Dodds James , Watmough Hugh , Gillman Jamie C. , Beevers David , Cassidy Simon , Short Ben , Metcalfe Paige , Lomas Michael J. , Graham-Kevan Draco , Gregory Samantha E. A. 
Published: December 2024

Abstract
The growing interest in harnessing natural environments to enhance mental health, including cognitive functioning and mood, has yielded encouraging results in initial studies. Given that images of nature have demonstrated similar benefits, they are frequently employed as proxies for real-world environments. To ensure precision and control, researchers often manipulate images of natural environments. The effectiveness of this approach relies on standardization of imagery, and therefore, inconsistency in methods and stimuli has limited the synthesis of research findings in the area. Responding to these limitations, the current paper introduces the Salford Nature Environments Database (SNED), a standardized database of natural images created to support ongoing research into the benefits of nature exposure. The SNED currently exists as the most comprehensive nature image database available, comprising 500 high-quality, standardized photographs capturing a variety of possible natural environments across the seasons. It also includes normative scores for user-rated (801 participants) characteristics of fascination, refuge and prospect, compatibility, preference, valence, arousal, and approach–avoidance, as well as data on physical properties of the images, specifically luminance, contrast, entropy, CIELAB colour space parameter values, and fractal dimensions. All image ratings and content detail, along with participant details, are freely available online. Researchers are encouraged to use this open-access database in accordance with the specific aims and design of their study. The SNED represents a valuable resource for continued research in areas such as nature-based therapy, social prescribing, and experimental approaches investigating underlying mechanisms that help explain how natural environments improve mental health and wellbeing.
Various Forms of Regional Policy Supervision in the Era of Broad Autonomy
Authors: PUTERA Meldy , SISKA  , DEWIATI Yayu , SITOHANG Erikjon , KRISTHY Mutia Evi , JOVI  , DODDY  
Published: December 2024

Purpose:
Following abolishing all controlling measures except repressive control, decentralisation triggered fundamental changes in local government administration. Tight control may obstruct regions' autonomy and hamper central-regional relations. Proportional control is indispensable for balancing local governments' freedom. All monitoring measures outside Act 32/2004 are unwarranted without improvement of legal instruments at the central government.
Methodology:
The method in the given text revolves around the historical analysis and constitutional references to explain the concept of autonomy in the Indonesian regional government system. It highlights the evolution of autonomy from the 1950 Provisional Constitution to the 1945 Constitution amendments, emphasizing the need for autonomy to prevent secessionist tendencies.
Findings:
Supervision in autonomy serves as a crucial control tool, impacting regional freedom and either fostering autonomy or promoting centralization, contingent on the government system. Historical patterns reveal strict centralization in regional policies, limiting regional autonomy.
The Associations Between Individual Anthropometric Measurements and Fracture Risk: A Mendelian Randomization Study
Authors: Song Yucheng , Zhou Jun , Tang Guanghua 
Published: December 2024

Background
The primary objective of this study was to investigate and substantiate the possible causal connections between measurements of body dimensions and the likelihood of experiencing fractures.

Materials and Methods
We employed a two-sample Mendelian randomization (MR) analysis to examine the associations between anthropometric measurements and two specific traits—bone mineral density and fracture risk. To ensure the credibility of our causal conclusions, we used the inverse variance weighted (IVW) method along with various sensitivity analyses.

Results

Our findings suggest a notable link between increased height and the likelihood of fractures. Specifically, employing the IVW method revealed that for every 10-cm increase in height, there was a 6.0% rise in fracture risk (odds ratio [OR], 1.06; 95% CI, 1.06–1.13;
P
=.0001). This outcome was further supported by both the weighted-median method and the MR-Egger method, with an OR of 1.10 (95% CI, 1.03–1.16;
P
=.0003) and an OR of 1.11 (95% CI, 1.08–1.17;
P
=.0020), respectively. No statistically significant associations were observed between other measurements, such as body mass index (BMI), waist-to-hip ratio adjusted for BMI, hip circumference adjusted for BMI, and waist circumference adjusted for BMI, and fracture risk. Sensitivity analyses, including MR-Egger regression's intercept test and multivariate testing, indicated no substantial presence of directional pleiotropy in instrumental variables, ensuring the stability and reliability of our analysis results.

Conclusion

Our study used MR to present genetic evidence supporting height as a distinct causal factor in fracture susceptibility. Our findings underscore the importance of incorporating anthropometric measurements into the development of strategies for preventing and treating osteoporosis. [
Orthopedics
. 2025;48(1):25–29.]
Comment faire du neuf avec du vieux : le Métadictionnaire médical multilingue, une nouvelle phase de la bibliothèque numérique Medica
Authors: Vincent Jean-François 
Published: December 2024

Le Métadictionnaire médical multilingue de la bibliothèque numérique Medica (Université Paris Cité) a été conçu, dans le cadre d’un appel à projets de Collex-Persée, dans la continuité de la politique documentaire de Medica. La numérisation de masse des premières décennies du xxie siècle ayant porté des fruits considérables, il est désormais attendu des bibliothèques numériques qu’elles produisent des services plus sophistiqués, comme celui-ci. Le Métadictionnaire doit être utile non seulement aux linguistes, mais aussi aux autres publics de Medica. Il doit permettre d’interroger l’ensemble de son corpus de dictionnaires. Il enrichit le corpus existant des dictionnaires les plus anciens du domaine médical et ajoute la recherche en grec. Il donne à voir une liste des vedettes du corpus. Par la structuration des articles de sept dictionnaires, il exploite les traductions et d’autres liens entre les mots et les locutions pour permettre une circulation enrichie dans le corpus. Tous les liens ainsi établis sont vérifiables aisément.
Entre comptes et contes. L’enquête de Tchekhov au bagne de Sakhaline
Authors: Schiltz Marie-Ange 
Published: December 2024

À l’été 1890, Anton Tchekhov mène, seul, un recensement « exhaustif » auprès de « dix milles » bagnards et colons du bagne de Sakhaline qui est, en cette fin de siècle, le plus important lieu de déportation russe. Les résultats de l’enquête sont accessibles sous le titre « L’Île de Sakhaline ». Tchekhov veut dénoncer l’injustice faite aux malheureux que l’Empire russe déporte en masse vers son Extrême-Orient. Ce grand de la littérature honnit l’opinion, il ambitionne de produire un témoignage « objectif », dire vrai, être crû ; pour ce faire, il choisit l’observation chiffrée. Le présent article s’attache à rendre compte de l’élaboration du projet, du déroulement de l’enquête, de la rédaction du rapport sans négliger les aléas de la collecte, les interrogations sur la fiabilité des réponses et la difficulté à faire face à la masse de données ; l’embarras de l’auteur à faire parler les chiffres est fort heureusement contrebalancé par de fulgurantes échappées littéraires La rédaction du compte-rendu de l’enquête est longue et laborieuse. Le livre paraît en 1895.
Réactivation du souvenir du massacre des bananeraies (1928) depuis la ville de Ciénaga : la surenchère mémorielle face au désengagement de l’État colombien (années 2000).
Authors: Fablet Noémi 
Published: December 2024

Voilà bientôt un siècle que fut perpétré le massacre des bananeraies (1928) dans la ville de Ciénaga (Magdalena, Colombie). Cet article constitue un exercice d’histoire du temps présent portant sur la ville caribéenne, théâtre de cet événement de répression étatique ; il aborde les questions mémorielles sous l’angle des politiques publiques. La réactualisation de cet épisode d’extrême violence au XXIe siècle invite à se demander comment l’État colombien et les autorités municipales se positionnent vis-à-vis de cet héritage violent, à l’ère de la gouvernance mémorielle. À travers l’observation des interactions entre ces politiques locales et nationales et une demande sociale cienaguera en pleine effervescence, il s’agit d’interroger les effets de ces politiques publiques, ou plutôt de leur quasi-absence, sur la mémoire du massacre des bananeraies.
De centre de torture à patrimoine de l’humanité. L’École de Mécanique de la Marine (ESMA) comme emblème de la mémoire
Authors: Feld Claudia 
Published: December 2024

Le centre clandestin de détention qui a fonctionné dans l’École de Mécanique de la Marine (ESMA) dans la ville de Buenos Aires a été l’un des plus actifs et connus de la dernière dictature Argentine (1976-1983). Malgré ses singularités, non généralisables à l’ensemble des centres clandestins de détention, l’ESMA est devenu avec le temps un emblème de la mémoire destiné à représenter d’une part les horreurs qui se sont déroulées pendant la dictature et d’autre part l’importance des droits humains pour la démocratie argentine. Cet article reconstruit et tente de conceptualiser ce « processus d’emblématisation » de 1984 jusqu’à aujourd’hui. Les plus importantes représentations du centre clandestin, ainsi que les débats publics à propos du lieu dans lequel il a fonctionné font l’objet d’une analyse approfondie. Ainsi, ce texte se centre sur quatre séquences particulières : les débuts de la transition (1984), les déclarations d’un agent de la répression de l’ESMA (1995), la transformation du site de l’ESMA en espace pour la mémoire (2004) et l’inauguration du Musée et lieu de mémoire ESMA (2015). Cette étude ouvre aussi des interrogations sur un plan plus général à propos des processus sociaux de la mémoire, leurs défis et leurs tensions.
William James’s Inquiry into Modes of Existence
Authors: Frigerio Christian 
Published: December 2024

The ontological turn is one of the most debated issues in contemporary anthropology, but what it means for anthropology to become ontological is rarely made clear. Bruno Latour’s suggestion that anthropology should revolve around “modes of existence” is arguably the most robust proposal to date, but the connection between modes of existence and properly anthropological concerns remains obscure. This paper argues that William James is a key figure for getting a better understanding of what ontology and modes of existence mean, and that in particular “The Perception of Reality” – chapter XXI of the second volume of the Principles of Psychology – offers many possibilities for linking a framework centered on human being-in-the-world, such as anthropology, with properly metaphysical concerns. In this paper, the content of “The Perception of Reality” is summarized, and James’s proposal to ground modes of existence on belief is discussed, to show how, once we are clear about what we mean by “ontology,” anthropology can (and should) engage in metaphysical debates in order to make its stakes explicit. The conclusion suggests that James’s grounding of modes of existence on individual belief can compensate for ontological anthropology’s tendency to erase the internal differences of a collective in order to speak of a supposedly unified and homogeneous “cosmology.”