bluuebunny/crossref_metadata_2025_split huggingface dataset:
Low expression of ANT1 confers oncogenic properties to rhabdomyosarcoma tumor cells by modulating metabolism and death pathways
Authors: Vial J. , Huchedé P. , Fagault S. , Basset F. , Rossi M. , Geoffray J. , Soldati H. , Bisaccia J. , Elsensohn M. H. , Creveaux M. , Neves D. , Blay J. Y. , Fauvelle F. , Bouquet F. , Streichenberger N. , Corradini N. , Bergeron C. , Maucort-Boulch D. , Castets P. , Carré M. , Weber K. , Castets M. 
Published: July 2020

AbstractRhabdomyosarcoma (RMS) is the most frequent form of pediatric soft-tissue sarcoma. It is divided into two main subtypes: ERMS (embryonal) and ARMS (alveolar). Current treatments are based on chemotherapy, surgery, and radiotherapy. The 5-year survival rate has plateaued at 70% since 2000, despite several clinical trials. RMS cells are thought to derive from the muscle lineage. During development, myogenesis includes the expansion of muscle precursors, the elimination of those in excess by cell death and the differentiation of the remaining ones into myofibers. The notion that these processes may be hijacked by tumor cells to sustain their oncogenic transformation has emerged, with RMS being considered as the dark side of myogenesis. Thus, dissecting myogenic developmental programs could improve our understanding of RMS molecular etiology. We focused herein on ANT1, which is involved in myogenesis and is responsible for genetic disorders associated with muscle degeneration. ANT1 is a mitochondrial protein, which has a dual functionality, as it is involved both in metabolism via the regulation of ATP/ADP release from mitochondria and in regulated cell death as part of the mitochondrial permeability transition pore. Bioinformatics analyses of transcriptomic datasets revealed that ANT1 is expressed at low levels in RMS. Using the CRISPR-Cas9 technology, we showed that reduced ANT1 expression confers selective advantages to RMS cells in terms of proliferation and resistance to stress-induced death. These effects arise notably from an abnormal metabolic switch induced by ANT1 downregulation. Restoration of ANT1 expression using a Tet-On system is sufficient to prime tumor cells to death and to increase their sensitivity to chemotherapy. Based on our results, modulation of ANT1 expression and/or activity appears as an appealing therapeutic approach in RMS management.
Olaparib for metastatic breast cancer in a patient with a germline PALB2 variant
Authors: Kuemmel Sherko , Harrach Hakima , Schmutzler Rita K. , Kostara Athina , Ziegler-Löhr Katja , Dyson Mark H. , Chiari Ouafaa , Reinisch Mattea 
Published: July 2020

AbstractThere is a strong biologic rationale that poly(adenosine diphosphate-ribose) polymerase (PARP) inhibitors may benefit a broader range of metastatic breast cancer (MBC) patients than covered by current approvals, which require a germline BRCA1/2 sequence variant affecting function. We report a patient with germline/somatic BRCA1/2 wild-type MBC, who had a dramatic response to the PARP inhibitor olaparib of at least 8 months’ duration. The patient is a 37-year-old woman with recurrent, hormone receptor-positive, HER2-negative MBC that had progressed despite hormonal therapy and palbociclib. Sensitivity to olaparib was likely conferred by a germline sequence variant affecting function in PALB2 (exon 1, c.18G>T, p.(=)). This case documenting activity of olaparib monotherapy in germline/somatic BRCA1/2 wild-type MBC illustrates that the clinical potential of PARP inhibition in MBC extends beyond currently approved indications to additional patients whose tumors have (epi)genetic changes affecting homologous recombination repair.
Colossal magnetoresistance in a nonsymmorphic antiferromagnetic insulator
Authors: Rosa Priscila , Xu Yuanfeng , Rahn Marein , Souza Jean , Kushwaha Satya , Veiga Larissa , Bombardi Alessandro , Thomas Sean , Janoschek Marc , Bauer Eric , Chan Mun , Wang Zhijun , Thompson Joe , Harrison Neil , Pagliuso Pascoal , Bernevig Andrei , Ronning Filip 
Published: July 2020

AbstractHere we investigate antiferromagnetic Eu5In2Sb6, a nonsymmorphic Zintl phase. Our electrical transport data show that Eu5In2Sb6 is remarkably insulating and exhibits an exceptionally large negative magnetoresistance, which is consistent with the presence of magnetic polarons. From ab initio calculations, the paramagnetic state of Eu5In2Sb6 is a topologically nontrivial semimetal within the generalized gradient approximation (GGA), whereas an insulating state with trivial topological indices is obtained using a modified Becke−Johnson potential. Notably, GGA + U calculations suggest that the antiferromagnetic phase of Eu5In2Sb6 may host an axion insulating state. Our results provide important feedback for theories of topological classification and highlight the potential of realizing clean magnetic narrow-gap semiconductors in Zintl materials.
Contagious Bovine and Caprine Pleuropneumonia: a research community’s recommendations for the development of better vaccines
Authors: Jores Joerg , Baldwin Cynthia , Blanchard Alain , Browning Glenn F. , Colston Angie , Gerdts Volker , Goovaerts Danny , Heller Martin , Juleff Nick , Labroussaa Fabien , Liljander Anne , Muuka Geoffrey , Nene Vish , Nir-Paz Ran , Sacchini Flavio , Summerfield Artur , Thiaucourt François , Unger Hermann , Vashee Sanjay , Wang Xiumei , Salt Jeremy 
Published: July 2020

AbstractContagious bovine pleuropneumonia (CBPP) and contagious caprine pleuropneumonia (CCPP) are major infectious diseases of ruminants caused by mycoplasmas in Africa and Asia. In contrast with the limited pathology in the respiratory tract of humans infected with mycoplasmas, CBPP and CCPP are devastating diseases associated with high morbidity and mortality. Beyond their obvious impact on animal health, CBPP and CCPP negatively impact the livelihood and wellbeing of a substantial proportion of livestock-dependent people affecting their culture, economy, trade and nutrition. The causative agents of CBPP and CCPP are Mycoplasma mycoides subspecies mycoides and Mycoplasma capricolum subspecies capripneumoniae, respectively, which have been eradicated in most of the developed world. The current vaccines used for disease control consist of a live attenuated CBPP vaccine and a bacterin vaccine for CCPP, which were developed in the 1960s and 1980s, respectively. Both of these vaccines have many limitations, so better vaccines are urgently needed to improve disease control. In this article the research community prioritized biomedical research needs related to challenge models, rational vaccine design and protective immune responses. Therefore, we scrutinized the current vaccines as well as the challenge-, pathogenicity- and immunity models. We highlight research gaps and provide recommendations towards developing safer and more efficacious vaccines against CBPP and CCPP.
Cranial morphology of the tanystropheid Macrocnemus bassanii unveiled using synchrotron microtomography
Authors: Miedema Feiko , Spiekman Stephan N. F. , Fernandez Vincent , Reumer Jelle W. F. , Scheyer Torsten M. 
Published: July 2020

AbstractThe genusMacrocnemusis a member of the Tanystropheidae, a clade of non-archosauriform archosauromorphs well known for their very characteristic, elongated cervical vertebrae. Articulated specimens are known from the Middle Triassic of Alpine Europe and China. Although multiple articulated specimens are known, description of the cranial morphology has proven challenging due to the crushed preservation of the specimens. Here we use synchrotron micro computed tomography to analyse the cranial morphology of a specimen of the type speciesMacrocnemus bassaniifrom the Besano Formation of Monte San Giorgio, Ticino, Switzerland. The skull is virtually complete and we identify and describe the braincase and palatal elements as well the atlas-axis complex for the first time. Moreover, we add to the knowledge of the morphology of the skull roof, rostrum and hemimandible, and reconstruct the cranium ofM. bassaniiin 3D using the rendered models of the elements. The circumorbital bones were found to be similar in morphology to those of the archosauromorphsProlacerta broomiandProtorosaurus speneri. In addition, we confirm the palatine, vomer and pterygoid to be tooth-bearing palatal bones, but also observed heterodonty on the pterygoid and the palatine.
The potential for a CRISPR gene drive to eradicate or suppress globally invasive social wasps
Authors: Lester Philip J. , Bulgarella Mariana , Baty James W. , Dearden Peter K. , Guhlin Joseph , Kean John M. 
Published: July 2020

AbstractCRISPR gene drives have potential for widespread and cost-efficient pest control, but are highly controversial. We examined a potential gene drive targeting spermatogenesis to control the invasive common wasp (Vespula vulgaris) in New Zealand. Vespula wasps are haplodiploid. Their life cycle makes gene drive production challenging, as nests are initiated by single fertilized queens in spring followed by several cohorts of sterile female workers and the production of reproductives in autumn. We show that different spermatogenesis genes have different levels of variation between introduced and native ranges, enabling a potential ‘precision drive’ that could target the reduced genetic diversity and genotypes within the invaded range. In vitro testing showed guide-RNA target specificity and efficacy that was dependent on the gene target within Vespula, but no cross-reactivity in other Hymenoptera. Mathematical modelling incorporating the genetic and life history traits of Vespula wasps identified characteristics for a male sterility drive to achieve population control. There was a trade-off between drive infiltration and impact: a drive causing complete male sterility would not spread, while partial sterility could be effective in limiting population size if the homing rate is high. Our results indicate that gene drives may offer viable suppression for wasps and other haplodiploid pests.
Prevalence and causes of vision impairment and blindness in the Russian ural eye and medical study
Authors: Bikbov Mukharram M. , Kazakbaeva Gyulli M. , Zainullin Rinat M. , Gilmanshin Timur R. , Nuriev Ildar F. , Zaynetdinov Artur F. , Yakupova Dilya F. , Uzianbaeva Yulia V. , Panda-Jonas Songhomitra , Mukhamadieva Svetlana R. , Khikmatullin Renat I. , Aminev Said K. , Arslangareeva Inga I. , Jonas Jost B. 
Published: July 2020

AbstractTo assess prevalence of mild vision impairment (MVI; best corrected visual acuity (BCVA) < 6/12 to 6/18 in the better eye), moderate-to-severe vision impairment (MSVI; BCVA < 6/18 but ≥ 3/60) and blindness (BCVA < 3/60) in a local population in Russia, we conducted the population-based Ural Eye and Medical Study. Out of 7,328 eligible individuals aged 40 + years, 5,899 (80.5%) individuals participated. MVI was present in 184 (3.1%; 95% confidence interval (CI) 2.7, 3.6) individuals, MSVI in 182 (3.1%; 95% CI 2.7, 3.5) individuals, and 11 individuals (0.19%; 95% CI 0.008, 0.30) were blind. Causes for MSVI were cataract (n = 109; 59.9%), late stage of age-related macular degeneration (n = 14; 7.7%; geographic atrophy and neovascular AMD in 7 (3.8%) individuals) each), myopic maculopathy (n = 11; 6.0%), glaucoma (n = 9; 4.9%), non-glaucomatous optic nerve damage (n = 5; 2.7%), and diabetic retinopathy (n = 4; 2.2%). Causes for blindness were cataract (n = 3; 27.3%), myopic maculopathy (n = 2; 18.2%), retinal dystrophies (n = 2; 18.2%), glaucoma (n = 1; 9.1%), and corneal scars (n = 1; 9.1%). Higher prevalence of MSVI/blindness was associated with age (P < 0.001; odds ratio (OR)1.10; 95% CI 1.08, 1.12), male gender (P < 0.001; OR 2.32; 95% CI 1.47, 3.66), educational level (P < 0.001; OR 0.83; 95% CI 0.76,0.92), manual grip force (P < 0.001; OR 0.94; 95% CI 0.92, 0.96), diabetes prevalence (P = 0.006; OR 1.67; 95% CI 1.08, 2.56) and axial length (P < 0.001; OR 1.43; 95% CI 1.26,1.62). In this population from Bashkortostan/Russia, prevalence of MVI, MSVI and blindness was 3.1%, 3.1% and 0.19%, respectively. Cataract was the most frequent cause of reversible vision impairment, while AMD, myopic maculopathy and glaucoma were the most common reasons for irreversible vision impairment.
The growing importance of oceanic moisture sources for continental precipitation
Authors: Gimeno Luis , Nieto Raquel , Sorí Rogert 
Published: July 2020

AbstractThe precipitation that falls on the continents defines the extent and nature of terrestrial ecosystems and human activity in them, all of which are adapted to and maintained by present-day precipitation. In essence, precipitation is supplied by moisture that either comes directly from the ocean, or is subsequently recycled from the continents themselves. Both the processes that control evaporation and the main mechanisms of moisture transport clearly differ between the ocean and the continent, thus within the context of a changing climate, it may be expected that the relationship between precipitation of oceanic and terrestrial origin varies globally and regionally, as will the influence of these two basic components of total precipitation on global and regional precipitation trends, especially in tropical regions. We describe an approach based on a Lagrangian technique for estimating the precipitation in a target region given the proportions of moisture transported from the two sources (ocean and continent) to reveal that the percentage of precipitation of oceanic origin has increased globally in the current climate (1980–2016). The greatest observed rate of increase is in the tropical regions; furthermore, the trends of precipitation in these regions are controlled by trends in precipitation for which the source of moisture is the ocean.
T cell-specific deletion of Pgam1 reveals a critical role for glycolysis in T cell responses
Authors: Toriyama Koji , Kuwahara Makoto , Kondoh Hiroshi , Mikawa Takumi , Takemori Nobuaki , Konishi Amane , Yorozuya Toshihiro , Yamada Takeshi , Soga Tomoyoshi , Shiraishi Atsushi , Yamashita Masakatsu 
Published: July 2020

AbstractAlthough the important roles of glycolysis in T cells have been demonstrated, the regulatory mechanism of glycolysis in activated T cells has not been fully elucidated. Furthermore, the influences of glycolytic failure on the T cell-dependent immune response in vivo remain unclear. We therefore assessed the role of glycolysis in the T cell-dependent immune response using T cell-specific Pgam1-deficient mice. Both CD8 and CD4 T cell-dependent immune responses were attenuated by Pgam1 deficiency. The helper T cell-dependent inflammation was ameliorated in Pgam1-deficient mice. Glycolysis augments the activation of mTOR complex 1 (mTORC1) and the T-cell receptor (TCR) signals. Glutamine acts as a metabolic hub in activated T cells, since the TCR-dependent increase in intracellular glutamine is required to augment glycolysis, increase mTORC1 activity and augment TCR signals. These findings suggest that mTORC1, glycolysis and glutamine affect each other and cooperate to induce T cell proliferation and differentiation.
Structure and function of a malaria transmission blocking vaccine targeting Pfs230 and Pfs230-Pfs48/45 proteins
Authors: Singh Kavita , Burkhardt Martin , Nakuchima Sofia , Herrera Raul , Muratova Olga , Gittis Apostolos G. , Kelnhofer Emily , Reiter Karine , Smelkinson Margery , Veltri Daniel , Swihart Bruce J. , Shimp Richard , Nguyen Vu , Zhang Baoshan , MacDonald Nicholas J. , Duffy Patrick E. , Garboczi David N. , Narum David L. 
Published: July 2020

AbstractProteins Pfs230 and Pfs48/45 arePlasmodium falciparumtransmission-blocking (TB) vaccine candidates that form a membrane-bound protein complex on gametes. The biological role of Pfs230 or the Pfs230-Pfs48/45 complex remains poorly understood. Here, we present the crystal structure of recombinant Pfs230 domain 1 (Pfs230D1M), a 6-cysteine domain, in complex with the Fab fragment of a TB monoclonal antibody (mAb) 4F12. We observed the arrangement of Pfs230 on the surface of macrogametes differed from that on microgametes, and that Pfs230, with no known membrane anchor, may exist on the membrane surface in the absence of Pfs48/45. 4F12 appears to sterically interfere with Pfs230 function. Combining mAbs against different epitopes of Pfs230D1 or of Pfs230D1 and Pfs48/45, significantly increased TB activity. These studies elucidate a mechanism of action of the Pfs230D1 vaccine, model the functional activity induced by a polyclonal antibody response and support the development of TB vaccines targeting Pfs230D1 and Pfs230D1-Pfs48/45.
Single channel Josephson effect in a high transmission atomic contact
Authors: Senkpiel Jacob , Dambach Simon , Etzkorn Markus , Drost Robert , Padurariu Ciprian , Kubala Björn , Belzig Wolfgang , Yeyati Alfredo Levy , Cuevas Juan Carlos , Ankerhold Joachim , Ast Christian R. , Kern Klaus 
Published: July 2020

AbstractThe Josephson effect in scanning tunneling microscopy (STM) is an excellent tool to probe the properties of a superconductor on a local scale. We use atomic manipulation in a low temperature STM to create mesoscopic single channel contacts and study the Josephson effect at arbitrary transmissions. We observe significant deviations from the Ambegaokar-Baratoff formula relating the critical current to the order parameter starting from transmissions of τ > 0.1. Using the full current-phase relation, we model the Josephson effect in the dynamical Coulomb blockade regime, where the charging energy of the junction capacitance cannot be neglected, and find excellent agreement with the experimental data. Projecting the current-phase relation onto the charge transfer operator shows that at high transmission, non-linear behaviour arises and multiple Cooper pair tunneling may occur. Our model includes these deviations, which become non-negligible in Josephson-STM, for example, when scanning across single adatoms.
Selective phase growth and precise-layer control in MoTe2
Authors: Fraser James P. , Masaityte Liudvika , Zhang Jingyi , Laing Stacey , Moreno-López Juan Carlos , McKenzie Adam F. , McGlynn Jessica C. , Panchal Vishal , Graham Duncan , Kazakova Olga , Pichler Thomas , MacLaren Donald A. , Moran David A. J. , Ganin Alexey Y. 
Published: July 2020

AbstractMinor structural changes in transition metal dichalcogenides can have dramatic effects on their electronic properties. This makes the quest for key parameters that enable a selective choice between the competing metallic and semiconducting phases in the 2D MoTe2 system compelling. Herein, we report the optimal conditions at which the choice of the initial seed layer dictates the type of crystal structure of atomically-thin MoTe2 films grown by chemical vapour deposition (CVD). When Mo metal is used as a seed layer, semiconducting 2H-MoTe2 is the only product. Conversely, MoO3 leads to the preferential growth of metallic 1T′-MoTe2. The control over phase growth allows for simultaneous deposition of both 2H-MoTe2 and 1T′-MoTe2 phases on a single substrate during one CVD reaction. Furthermore, Rhodamine 6G dye can be detected using few-layered 1T′-MoTe2 films down to 5 nM concentration, demonstrating surface enhanced Raman spectroscopy (SERS) with sensitivity several orders of magnitude higher than for bulk 1T′-MoTe2.
Catalysis-free transformation of non-graphitising carbons into highly crystalline graphite
Authors: Fogg Jason L. , Putman Kate J. , Zhang Tianyi , Lei Yu , Terrones Mauricio , Harris Peter J. F. , Marks Nigel A. , Suarez-Martinez Irene 
Published: July 2020

AbstractHigh-purity graphite is a sought-after material for lithium-ion batteries and graphene production. Most organic materials do not graphitise upon heating unless a metal catalyst is present. The catalyst becomes embedded in the graphite and is difficult to remove. Here, we present a catalysis-free technique capable of producing highly crystalline graphite from materials generally considered incapable of this transformation. Using the furnace inside an Atomic Absorption Spectrometer, we perform repeated high-temperature pulsing of polyvinylidene chloride followed by analysis with Raman, X-ray diffraction and transmission electron microscopy. Unexpectedly, ~90% of the sample transforms into highly ordered graphite with very few defects. A combustion route is proposed in which oxygen attacks the structural units that inhibit graphitisation. We apply the same approach to cellulose and obtain ten times more ordered material than conventional furnaces, confirming that polyvinylidene chloride is not an isolated case. Potentially, this method could be used to synthesise graphite from any organic material, including waste sources such as biomass.
Solution for controlling a hydraulic motor using cloud data
Authors: Udrea Ioana , Gheorghe Viorel Ionut , Avram Mihai , Petrache Silviu , Popescu-Cuta Alina , Popa Romeo Traian 
Published: July 2020

In the last period, Internet of Things (IoT) is experiencing an increasing development, and the tendency of hosting applications and especially data, in cloud computing systems is growing. Currently, most of the Building Management System (BMS) analyse directly data or transfer it to the cloud.In this context, the proposed solution aims to read indoor thermal climate data from cloud computing platform and based on them operate a hydraulic motor. The input data could be generated by an in-house sensor monitoring solution or by a foreign BMS system that transmits open source data to cloud.In this study some conditions are imposed to the input data and as a result, control current is generated and transmitted in order to command proportional electro-hydraulic valve that drive a hydraulic motor. The electro-hydraulic valve is controlled with the help of an amplifier, by a data acquisition board (DAQ) that runs LabView application. With the help of the developed software, the motor speed and the direction of rotation can be controlled.In last years, the number of requests for architectures projects with moveable elements has increased because they multiply the possible use of the buildings. With the information generated by the sensors that measure the indoor climate parameters, the hydraulic motor could be used in such a construction. Examples of atypical buildings with special functionalities that the architects would propose and that needs high torque actuation are presented in this paper.
An analysis of baffles designs for limiting impact on the directional response for a partially filled tank with agro-food liquids
Authors: Milian Gabriela , Biriş Sorin-Ştefan , Ipate George , Hristea Alexandru , Sorica Cristian , Nenciu Florin 
Published: July 2020

A directional response for a partially filled tank with agro-food liquids incorporates the motion of the free surface due to roll and longitudinal accelerations into the dynamic tank model. This paper presents an analysis of efficaciousness of different designs of baffles for two different tank, to limit the impact of the response given by the liquid on the tank walls. In this paper modelling of the behaviour of the cross-section circular and ellipsoidal tank with baffles, partially filled with liquids agro-food, was developed, which performs a braking-in-a-turn manoeuver. Two different tank truck models have been used to simulate the sloshing effect of agro-food liquid cargo. Is also studied the two geometric models of the tank was done by using SolidWorks and simulating the behaviour of the tank ANSYS-CFX.
In this study, there were simulated and modeled two models of tank-type “X”, respectively type “Y” used for the storage and transport of liquid agro-food products, namely water, for different speeds (v = 14m / s and v = 20m / s) and two filling levels of the tank trucks (40% and 80%). The results obtained were compared the behaviour of the tank partially filled into a braking-in-a-turn manoeuver, in which the total values of the deformations that appear as a result of the impact due to the movement of the liquid inside the tank trucks.