bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptomic study in women with trisomy 21 identifies a possible role of the GTPases of the immunity-associated proteins (GIMAP) in the protection of breast cancer
Authors: Mégarbané André , Piquemal David , Rebillat Anne-Sophie , Stora Samantha , Pierrat Fabien , Bruno Roman , Noguier Florian , Mircher Clotilde , Ravel Aimé , Vilaire-Meunier Marie , Durand Sophie , Lefranc Gérard 
Published: June 2020

AbstractBackground: People with trisomy 21 (T21) are predisposed to developing hematological tumors, but have significantly lower-than-expected age-adjusted incidence rates of having a solid tumor. Material and methods: To identify novel genetic factors implicated in the lower breast cancer (BC) frequency observed in women with T21 than in the general population, we compared the transcriptome pattern of women with a homogeneous T21, aged more than 30 years, with or without BC, and tumoral BC tissue of control women with a normal karyotype from the study of Varleyet al. (2014). Results: Differential analysis of gene expression between the 15 women in the T21 without BC group and BC patients in the other groups (two women with T21 and fifteen control women, respectively) revealed 154 differentially expressed genes, of which 63 were found to have similar expression profile (up- or downregulated). Of those 63 genes, four were in the same family, namelyGIMAP4,GIMAP6,GIMAP7andGIMAP8, and were strongly upregulated in the T21 without BC group compared to the other groups. A significant decrease in mRNA levels of these genes in BC tissues compared to non-tumor breast tissues was also noted. Conclusion: We found that the expression of someGIMAPsis significantly higher in women with T21 without BC than in patients with sporadic BC. Our findings support the hypothesis that GIMAPs may play a tumor-suppressive role against BC, and open the possibility that they may also have the same role for other solid tumors in T21 patients. The search for new prognostic factors and hopefully new therapeutic or preventive strategies against BC are discussed.
Regulation of the miR-19b-mediated SOCS6-JAK2/STAT3 pathway by lncRNA MEG3 is involved in high glucose-induced apoptosis in hRMECs
Authors: Xiao Fan , Li Lan , Fu Jing-Song , Hu Yu-Xiang , Luo Rong 
Published: June 2020

Abstract
Objective: Diabetic retinopathy (DR) is one of the most severe and common complications of diabetes mellitus. The present study aimed to investigate the molecular mechanism of MEG3, miR-19b and SOCS6 in human retinal microvascular endothelial cells (hRMECs) under high glucose conditions. Methods: HRMECs were cultured in 5 or 30 mM D-glucose medium. qRT-PCR and Western blotting were used to determine the mRNA expression and protein levels. MTT assay and flow cytometry analysis were performed to detect the viability and apoptosis of hRMECs, respectively. TNF-α, IL-6 and IL-1β levels in cell supernatants were detected by ELISA. The activity of caspase-3/7 was also determined. A luciferase reporter assay was performed to confirm the targeting relationship between miR-19b and SOCS6, as well as MEG3 and miR-19b. Results: Our study demonstrated that miR-19b was increased and SOCS6 was decreased in HG-induced hRMECs. Knockdown of SOCS6 inhibited cell viability and reversed the promotion of cell viability induced by knockdown of miR-19b. Additionally, miR-19b directly targeted and negatively regulated SOCS6. Moreover, miR-19b promoted the cell apoptosis rate and caspase-3/7 activity and increased inflammatory factors through the SOCS6-mediated JAK2/STAT3 signalling pathway. In addition, MEG3 attenuated HG-induced apoptosis of hRMECs by targeting the miR-19b/SOCS6 axis. Conclusion: These findings indicate that MEG3 inhibited HG-induced apoptosis and inflammation by regulating the miR-19b/SOCS6 axis through the JAK2/STAT3 signalling pathway in hRMECs. Thus, these findings might provide a new target for the treatment of DR.
High expression of AK1 predicts inferior prognosis in acute myeloid leukemia patients undergoing chemotherapy
Authors: Qin Tong , Zhao Hongmian , Shao Yunli , Hu Ning , Shi Jinlong , Fu Lin , Zhang Yijie 
Published: June 2020

Abstract
The purpose of the present study was to investigate whether expression levels of adenylate kinase 1 (AK1) were associated with prognosis of acute myeloid leukemia (AML) in patients treated with chemotherapy or allogeneic hematopoietic stem cell transplantation (allo-HSCT). 85 AML patients with AK1 expression report who received chemotherapy-alone and 71 who underwent allo-HSCT from The Cancer Genome Atlas database were identified and grouped into either AK1high or AK1low based on their AK1 expression level relative to the median. Then, overall survival (OS) and event-free survival (EFS) were compared between patients with high vs. low AK1 expression. In the chemotherapy group, high AK1 expression was favorable for both EFS (P=0.016) and OS (P=0.014). In the allo-HSCT group, there was no association for AK1 expression levels and clinical outcomes. Further analyses suggested that in the high AK1 expression group, EFS and OS were longer in patients treated with allo-HSCT compared with those treated with chemotherapy (P=0.0011; P&lt;0.0001, respectively), whereas no significant differences were observed in the low AK1 expression group. In summary, we reported AK1 as an independent unfavorable prognostic factor of AML patients undergoing chemotherapy, and its use could also facilitate clinical decision-making in selecting treatment for AML patients. Patients with high AK1 expression may be recommended for early allo-HSCT.
Personal Audio System: Hearing Symptoms, Habits, and Sound Pressure Levels Measured in Real Ear and a Manikin
Authors: Almeida Tamara R. , Rocha Clayton H. , Rabelo Camila M. , Gomes Raquel F. , Neves-Lobo Ivone F. , Samelli Alessandra Giannella 
Published: June 2020

Purpose
The aims of this study were to characterize hearing symptoms, habits, and sound pressure levels (SPLs) of personal audio system (PAS) used by young adults; estimate the risk of developing hearing loss and assess whether instructions given to users led to behavioral changes; and propose recommendations for PAS users.

Method
A cross-sectional study was performed in 50 subjects with normal hearing. Procedures included questionnaire and measurement of PAS SPLs (real ear and manikin) through the users' own headphones and devices while they listened to four songs. After 1 year, 30 subjects answered questions about their usage habits. For the statistical analysis, one-way analysis of variance, Tukey's post hoc test, Lin and Spearman coefficients, the chi-square test, and logistic regression were used.

Results

Most subjects listened to music every day, usually in noisy environments. Sixty percent of the subjects reported hearing symptoms after using a PAS. Substantial variability in the equivalent music listening level (Leq) was noted (
M
= 84.7 dBA; min = 65.1 dBA, max = 97.5 dBA). A significant difference was found only in the 4-kHz band when comparing the real-ear and manikin techniques. Based on the Leq, 38% of the individuals exceeded the maximum daily time allowance. Comparison of the subjects according to the maximum allowed daily exposure time revealed a higher number of hearing complaints from people with greater exposure. After 1 year, 43% of the subjects reduced their usage time, and 70% reduced the volume. A volume not exceeding 80% was recommended, and at this volume, the maximum usage time should be 160 min.

Conclusions
The habit of listening to music at high intensities on a daily basis seems to cause hearing symptoms, even in individuals with normal hearing. The real-ear and manikin techniques produced similar results. Providing instructions on this topic combined with measuring PAS SPLs may be an appropriate strategy for raising the awareness of people who are at risk.

Supplemental Material

https://doi.org/10.23641/asha.12431435
Staphylococcus aureus Pneumonia in the Community
Authors: He Hangyong , Wunderink Richard G. 
Published: June 2020

Abstract
Staphylococcus aureus is an emergent etiology of community-acquired pneumonia (CAP) over the past 2 decades, with severe community-acquired pneumonia (SCAP) caused by methicillin-resistant S. aureus (MRSA) leading to critical illness and death. S. aureus colonization is associated with a high incidence of pneumonia. Panton-Valentine leukocidin (PVL) is one of the most important virulence factors of S. aureus associated with serious complications. In recent years, community-associated MRSA (CA-MRSA) clones that caused infections in young adults and healthy individuals with no exposure to health care settings and no classical risk factors have emerged. Clinical features at admission including concurrent influenza infection, hemoptysis, multilobar infiltrates, and neutropenia should suggest S. aureus CAP. Sputum Gram stains, cultures (or tracheobronchial aspirates or bronchoalveolar lavage in mechanically ventilated patients), polymerase chain reaction (nasopharyngeal or oropharyngeal or lower respiratory tract specimens), and two sets of blood cultures should be obtained from patients presenting with severe S. aureus CAP. For CAP due to methicillin-susceptible S. aureus, first-line therapy is usually cefazolin, oxacillin, or ceftaroline. For CA-MRSA pneumonia, linezolid is recommended. If vancomycin or teicoplanin are used, combination with clindamycin or rifampicin should be considered in cases of PVL-positive MRSA CAP.
Manejo de falha óssea em fratura da região distal do fêmur pela técnica de Masquelet com enxerto de fíbula associado a enxerto de crista ilíaca: Relato de dois casos
Authors: Pimenta Frederico Silva , Prudêncio Tardelli Lapaz da Silva , Ferreira Gustavo Pacheco Martins , de Barros Alessandro Cordoval , Caldas Marco Túlio Lopes , Rodrigues Heitor Maia 
Published: June 2020

ResumoDois casos de falha óssea após fratura da região distal do fêmur tratados pela técnica de Masquelet são apresentados. O primeiro caso envolve uma perda óssea aguda, e o segundo, uma pseudoartrose. O manejo adequado dessas lesões levou à consolidação e a um bom resultado funcional.
Cleft Nucleus Lymphocytosis in Young Infants with Pertussis
Authors: Zhu Huifang , Liao Hongqun , Zhong Xiaoming , Rao Xingyu , Yang Xin , Luo Kaiyuan 
Published: June 2020

AbstractThis study aims to assess whether the cleft nucleus lymphocytosis could be an early promising clue for the diagnosis of pertussis in young infants. Pertussis (whooping cough) is a severe respiratory disease mainly caused by Bordetella pertussis infection and is characterized by a significant rise in the number of leukocyte and lymphocyte in infants and young children. In this study, the Bordetella pertussis DNA was detected from samples of pharyngeal swab by PCR assay. Levels of serum specific IgM against other respiratory pathogens were detected by Enzyme-linked immunosorbent assay (ELISA) assay. The routine blood test including numbers of leukocytes, lymphocytes, and platelets etc. were tested by automatic hemocyte analyzer (Sysemx XN1000). Besides, the morphology of leucocytes was observed in peripheral blood smear with microscope by Wright-Giemsa stain. Three cases of pertussis with cleft nucleus lymphocytes in young infants were discussed in in the neonatal/pediatric intensive care unit in our hospital. Leukocytosis characterized by lymphocytes, as well as thrombocytosis were observed in all patients. Our results demonstrated that cleft nucleus lymphocytosis accompanied with leukocytosis and lymphocytes would be potent assistant indicators for the early diagnosis of pertussis in young children.
Acute Gonococcal Osteomyelitis and Arthritis of Hip: An Interesting Case and Review of Literature
Authors: Agarwal Nikki , Abughali Nazha , Chotikanatis Kobkul 
Published: June 2020

AbstractGonorrhea is the most common sexually transmitted disease globally, affecting both men and women equally. It has a myriad of presentations which can vary from local disease to disseminated gonococcal infection. We are reporting the first case of gonococcal arthritis leading to acute osteomyelitis of the femoral head in a young adult with Charcot-Marie-Tooth disease that occurred over a period of 2 weeks. Gonorrhea can affect joints and cause arthritis, and there have been case reports of untreated gonococcal arthritis leading to osteomyelitis after months. Here we report the first case of acute gonococcal spread to the bones causing osteomyelitis in a young adult. A high index of suspicion is needed to recognize this condition which if left unnoticed, can lead to erosive bone damage, and severe consequences.
Association of Cerclage with Composite Adverse Outcomes among Women Delivered at 36 Weeks or Later
Authors: Stephens Angela J. , Chen Han-Yang , Chauhan Suneet P. , Sibai Baha 
Published: June 2020

Objective This study aimed to compare the maternal and neonatal adverse outcomes among singletons delivered at 36 weeks or later with cerclage during index pregnancy versus those without cerclage.Study Design This retrospective cohort study utilized the U.S. vital statistics datasets from 2011 to 2013. Inclusion criteria were women with nonanomalous singletons, with and without cerclage placement, without diabetes or hypertensive disorders, and delivered at 36 to 41 weeks. The coprimary outcomes were composite maternal and neonatal adverse outcomes. Composite maternal adverse outcome included admission to intensive care unit, maternal transfusion, ruptured uterus, unplanned hysterectomy, or unplanned operating room procedure. Composite neonatal adverse outcome included Apgar score less than 5 at 5 minutes, assisted ventilation for more than 6 hours, neonatal seizure, birth injury, or neonatal death. Secondary outcomes were chorioamnionitis and cesarean delivery. Multivariable Poisson's regression models with error variance were used while adjusting for confounders. Adjusted relative risk (aRR) with 95% confidence intervals (CIs) were calculated.Results Of the 8,508,228 women who met inclusion criteria, 0.2% had a cerclage and reached 36 weeks. Composite maternal (aRR: 2.04; 95% CI: 1.76–2.36) and neonatal (aRR: 1.28; 95% CI: 1.11–1.47) adverse outcomes were significantly higher among those with cerclage than those without cerclage. Chorioamnionitis (aRR: 1.47; 95% CI: 1.30–1.67) and cesarean delivery (aRR: 1.10; 95% CI: 1.08–1.12) were also significantly higher in women with cerclage than those without cerclage.Conclusion There is an association between increased composite maternal and neonatal adverse outcomes among women with cerclage who delivered at 36 to 41 weeks as compared with those without cerclage.Key Points
Extended Duration Ketamine Infusions in Critically Ill Children: A Case Report and Review of the Literature
Authors: Moore Eszter , Mayes Rebecca , Harkin Maura , Miller Jamie L. , Johnson Peter N. 
Published: June 2020

AbstractKetamine is an N-methyl-D-aspartate receptor antagonist that has been used as an adjunct analgesic and sedative in critically ill children. Previous reports noted that ketamine has been used for a variable duration of 12 to 408 hours for this indication. We report on the use of ketamine infusions for >720 hours as a second-line sedative in addition to an opioid and dexmedetomidine infusion in a 2-month old and 17-month old. The purpose of this case report and review of the literature is to highlight the prolonged ketamine exposure of these two patients and to provide awareness to clinicians on the potential of withdrawal with extended ketamine administration. These children were started on initials doses of 5 and 15 µg/kg/min and titrated to peak doses of 20 and 25 µg/kg/min, respectively. They were continued for a total of 987 and 792 hours, respectively. No adverse events were noted during the ketamine infusions. One patient developed possible withdrawal symptoms 17 hours after ketamine discontinuation despite tapering of the infusion. These symptoms resolved with administration of as needed intravenous opioids and benzodiazepines, and the agitation normalized within 24 hours after ketamine discontinuation. Clinicians should consider tapering ketamine infusions in children receiving >72 hours of a continuous infusion by 5 µg/kg/min every 8 to 12 hours. Patients should be monitored for potential withdrawal symptoms including anxiety, allodynia, hyperalgesia, sweating, and drowsiness.
Association between rs1421085 and rs9939609 Polymorphisms of Fat Mass and Obesity-Associated Gene with High-Density Lipoprotein Cholesterol and Triglyceride in Obese Turkish Children and Adolescents
Authors: Inandiklioğlu Nihal , Yaşar Adem 
Published: June 2020

AbstractSeveral studies have shown that rs9939609 and rs1421085 in fat mass and obesity-associated (FTO) gene rs17782313 and rs12970134 in melanocortin-4 receptor (MC4R) gene influence obesity. In the present study, we aimed to determine association between rs9939609, rs1421085, rs17782313, and rs12970134 polymorphism, and their relation with body mass index (BMI), glucose, insulin, homeostasis model assessment of insulin resistance (HOMA-IR) and lipid values in obese children. We included 100 newly diagnosed obese children and 100 healthy children. The rs1421085 (CC/CT) (p = 0.019) and rs9939609 (AA/AT) (p = 0.002) polymorphism regions were higher in the obese group. Additionally, we found that both the rs1421085 (CC/CT) and rs9939609 (AA/AT) polymorphism associated with high-density lipoprotein cholesterol (p = 0.011 and p = 0.003) and triglycerides (p = 0.01 and p = 0.004) level, respectively. Further, the rs9939609 and rs1421085 variants of FTO gene associated with HDL-cholesterol and triglycerides levels in obese children; however, updated studies with a large sample size are required to establish strong links with genetic variants and risk factors in childhood obesity.
Application of Banach Contraction Principle in Complex Valued Rectangular b-Metric Space
Authors: Sunarsini  , Biahdillah Aufa , Surjanto Sentot Didik 
Published: June 2020

Abstract
In this paper, we introduce the new generalization of metric space called complex valued rectangular b-metric space. This space generalize the triangle inequality properties to rectangular inequality with a constant s ≥ 1 and partial order concept in complex numbers. We also give a real example of Banach contraction principle in complex valued rectangular b-metric space at linear equation system, especially in electrical circuit.
Modeling of infant mortality in west sulawesi using zero inflated poisson regression method
Authors: Annisa R , Permatasari EO , Rumiati A T 
Published: June 2020

Abstract
The number of infant deaths, one of which can be reflected in the Infant Mortality Rate (IMR) is one indicator to determine the development of a country’s development. The AKB target in the 2015-2019 National Medium-Term Development Plan is 24 deaths per 1,000 live births. Based on the SDKI 2017, the IMR value in West Sulawesi is 42 deaths per 1,000 live births. Factors suspected of affecting infant mortality in West Sulawesi are not only in the health but also in socio-economic fields. Data on the number of infant mortality in West Sulawesi is a data count that contains a value of 0. Therefore, the analysis used is Zero Inflated Poisson (ZIP) Regression to determine what factors influence infant mortality in West Sulawesi. The results of the ZIP regression analysis showed that the best model with the smallest AIC and AICc is 324,3407 and 326,7407 were models with a combination of the variables of the percentage of active maternal and child health services, percentage of society with good drinking water adequate access and percentage of society with proper sanitation access. The variable that has a significant effect on the log model is the percentage of active maternal and child health services, percentage of society with good drinking water adequate access and percentage of society with proper sanitation access. In the variable logit model that is influential is the percentage of active maternal and child health services.
Design and application of reuse stage algorithm of case-based reasoning method on container stowage planning
Authors: Pratama A , Mukhlash I , Nugroho S 
Published: June 2020

Abstract
Stowage planning is a container arrangement planning activity on container ships. The Case-Based Reasoning method can solve the newest stowage plan cases by taking a stowage plan case that has the highest similarity and has been resolved in the past as a reference for arranging. The Case-Based Reasoning method has four-stage, there are Retrieve, Reuse, Revise, Retain. The Reuse stage is to adapt the Container Loading List so that it is arranged to resemble the case that was taken at the Retrieve stage. Algorithms for the Reuse stage of the Case-Based Reasoning Method has been designed and obtained the Container Loading List Adaptation, Fill-Blank, Adding the Remaining Container, Trim Optimization, and GM Optimization Functions. The functions can be implemented in software design and automatically or manually arranged the containers based on Casebase. Hence, users make the new stowage plans easier and faster. Based on the results of software testing, the difference between LCB and LCG is less than 1% LBP so that the Trim was fulfilled, and GM obtained a positive value so that the ship was in a stable condition.
The theft criminality forecasting in the surabaya district police region using poisson GARMA model and negative binomial GARMA model
Authors: Wardhani L P , Indrawati D , Wahyuningsih N , Setiawan  , Suhartono  , Kuswanto H 
Published: June 2020

Abstract
Theft is a crime that often occurs in the community. According to the Police, theft consists of ordinary theft, petty theft, weight theft, and theft of violence. According to BPS, the highest number of incidents of theft occurred during 2018, one of which was in the Surabaya City Police Region. Based on these facts forecasting is done to provide material for consideration of the number of incidents of theft in the future. Forecasting generally uses the ARMA model. The ARMA model is less suitable for use in count data. The ARMA model was developed into the GARMA model if the data is assumed to be a Poisson distribution called the Poisson GARMA model. If the data is assumed to be a Negative Binomial distribution is called the Negative Binomial GARMA model. The estimation of the Poisson GARMA model and the Binomial Negative GARMA model uses MLE and is optimized using IRLS. This model is applied in cases of theft crimes in the Surabaya City Police Region and compares the accuracy of forecasting between the Poisson GARMA model and the Negative Binomial GARMA model. Based on the analysis carried out, the Negative Binomial GARMA (1.0) model is better for Central Surabaya, East Surabaya, and South Surabaya than Poisson GARMA (1.0) model. The Negative Binomial GARMA (0.2) model is better for the West Surabaya region rather than the Poisson GARMA (0.2) model. The best model in the Surabaya City Police Region area is the Binomial Negative GARMA (0.2) model in the West Surabaya region. The selection of the best model uses the lowest value of AIC.
Maximizing operating speed of an interval control system with a robust controller on a base of a root approach to synthesis
Authors: Gayvoronskiy S A , Ezangina T , Khozhaev I 
Published: June 2020

Abstract
The paper is dedicated to a problem of improving operating speed of control systems with interval parameters. Solution of the problem reduces to finding values of controller parameters providing minimal setting time of a system with the worst combination of values of interval parameters. Controller synthesis is proposed to be performed on a base of interval characteristic polynomial of a system on a base of maximal stability degree criterion. The research resulted in a synthesis algorithm based on an interval extension of mathematical programming method. Example of synthesizing a robust controller providing maximal operating speed for a system with interval parameters via the algorithm is also given.