bluuebunny/crossref_metadata_2025_split huggingface dataset:
FAST 112 HEROES: A kindergarten-based educational stroke intervention for the whole family
Authors: Tsakpounidou Kalliopi , Loutrari Ariadne , Tselekidou Freideriki , Baskini Maria , Proios Hariklia 
Published: April 2020

Background: Stroke is a worldwide leading cause of disability, and around 50 million people are affected by stroke annually. Public stroke educational and awareness programmes can make a great deal of difference. Young children are in key position to seek urgent medical care if grandparents suffer a stroke, as grandparents are often their secondary caretakers. Objective: The objective of the current study was to design an educational intervention targeting children and, in parallel, directly involve extended family members. Design: Participatory action research. Setting: School-based education stroke intervention in Thessaloniki, Northern Greece. Methods: Over the course of 5 weeks, this intervention sought to educate 66 preschool children and their families through a series of novel activities that revolved around 4 superheroes, the FAST mnemonic and a medical emergency number. One superhero and their unique superpower was introduced every week through a Powerpoint presentation, a short animation video and a wide range of in-class and take-home activities, such as ‘phantom speech’, role-playing, funny face mimicking games, and rhyming poems. Children were also encouraged to identify their own family superhero so as to transfer their learning to real life. Results: Follow-up individual and group assessment pointed to encouraging results. Results from odd-one-out-tasks revealed that children were able to recognise the stroke symptoms in question. However, they performed more poorly on more complex tasks involving recall. Conclusions: Preschool children acquired knowledge of stroke symptoms which appears more solid when recognition is assessed. Assessment tasks involving substantial recall of information do not necessarily reflect the ability to detect stroke symptoms.
Assessment of routine diet (garlic consumption) as a pre- and postoperative protocol in oral and maxillofacial surgical interventions: An evidence-based literature review
Authors: Malhotra Upasna , Hasday Matthew , Romanos Georgios E , Javed Fawad 
Published: April 2020

Background: It was hypothesized that pathological bleeding (PB) during and/or after oral surgical procedures is higher in systemically healthy patients who have a history of garlic intake compared to controls (patients without a history of garlic consumption). Aim: The aim of the present review article was to identify studies in which garlic consumption was included as a patient management protocol before and after oral and maxillofacial surgery (OMFS). Methods: A review of pertinent indexed literature was performed. The focused question that was addressed was “Has diet (garlic consumption) been considered as a patient management protocol before and after OMFS?” The inclusion criteria were: (a) studies published in indexed databases, (b) original studies, (c) studies on OMFS, and (d) studies that considered diet (garlic consumption) as a patient management protocol before and after OMFS. Literature review, commentaries, letters to the editor, and studies published in non-indexed resources were excluded. The pattern of the present review was customized to summarize the pertinent information. Results: The initial search using the terms “oral,” maxillofacial,” “protocol,” and “surgery” yielded 1478 studies. The addition of the term “diet” to this search strategy reduced the number of studies to five. Further filtration of these studies using the terms “garlic” and/or “ Allium sativum” showed no studies. Conclusions: Garlic intake predisposes patients to PB. However, there are no studies in the indexed literature that have considered the inclusion of garlic consumption in patient management protocols before and after OMFS.
A Systematic Review of Culturally Adapted Behavioral Activation Treatments for Depression
Authors: Lehmann David C. , Bördlein Christoph 
Published: April 2020

Objective: Behavioral activation constitutes a promising behavioral treatment for depression. Due to its contextual and idiographic approach, the intervention might be well suited for treating depression in culturally diverse populations. Method: The authors conducted a systematic literature review on culturally adapted behavioral activation treatments. Results: Seventeen studies were identified through database searching involving different target populations and a variety of adapted interventions. Circumstances were frequently shaped by cultural values and a wide range of environmental stressors. Adaptations were found in different dimensions including language, content, methods, and context. Across studies, results indicated the effectiveness of behavioral activation and its cultural adaptations for treating depression in their respective target groups. Discussion: The results of this review may serve as an input both for practitioners employing behavioral activation in their daily work with culturally diverse clients and for researchers interested in culturally adapting treatment to specific populations.
Comparison of immunogenic markers of human chondrocytes and chondroprogenitors derived from non-diseased and osteoarthritic articular cartilage
Authors: Vinod Elizabeth , Ramasamy Boopalan , Kachroo Upasana 
Published: April 2020

Background: In the field of cartilage repair, cell-based therapy, employing the use of mesenchymal stem cells and chondrocytes maybe a propitious treatment option. Once transferred, success of transplanted cells is determined by their immunogenicity making assessment imperative. Another contender in the field, may be articular chondroprogenitors (CPs) shown to possess chondrogenic potential and reduced expression of hypertrophy markers. Our aim was to assess immunogenic properties of CPs (non-diseased and osteoarthritic (OA)) and compare them with chondrocytes since it may be a good alternative for cell-based therapy and data regarding its immunogenic profile is limited. Methods: Human chondrocytes and CPs from the same cartilage source were isolated. Passage 0 cells characterized by fluorescence-activated cell sorting against human surface antigen were human leucocyte antigen class I (HLA-A2 and HLA-B7), HLA-DR and its costimulatory molecules (CD80 and CD86) and macrophage/monocyte marker (CD14). Results and conclusion: Our observations indicated that CPs isolated from human non-diseased and OA cartilage showed similar immunogenic properties to chondrocytes isolated from the same source with no significant difference in expression. High-to-moderate expression of MHC class I (HLA-A2 and HLA-B7) and moderate-to-low expression of MHC class II (HLA-DR and its co-stimulatory molecules CD80 and CD86) were observed. This is the first report to shed insight on the immunogenic properties of human cartilage-derived progenitors, a potential contender in the field of regenerative therapy for formation of genuine hyaline cartilage.
BTK inhibitor therapy is effective in patients with CLL resistant to venetoclax
Authors: Lin Victor S. , Lew Thomas E. , Handunnetti Sasanka M. , Blombery Piers , Nguyen Tamia , Westerman David A. , Kuss Bryone J. , Tam Constantine S. , Roberts Andrew W. , Seymour John F. , Anderson Mary Ann 
Published: April 2020

Abstract
Highly active BTK inhibitors (BTKis) and the BCL2 inhibitor venetoclax have transformed the therapeutic landscape for chronic lymphocytic leukemia (CLL). Results of prospective clinical trials demonstrate the efficacy of venetoclax to salvage patients with disease progression on BTKis, but data on BTKi therapy after disease progression on venetoclax are limited, especially regarding durability of benefit. We retrospectively evaluated the records of 23 consecutive patients with relapsed/refractory CLL who received a BTKi (ibrutinib, n = 21; zanubrutinib, n = 2) after stopping venetoclax because of progressive disease. Median progression-free survival (PFS) and median overall survival after BTKi initiation were 34 months (range, &lt;1 to 49) and 42 months (range, 2-49), respectively. Prior remission duration ≥24 months and attainment of complete remission or undetectable measurable residual disease on venetoclax were associated with longer PFS after BTKi salvage (P = .044 and P = .029, respectively). BTKi therapy achieved durable benefit for patients with the BCL2 Gly101Val venetoclax resistance mutation (estimated 24-month PFS, 69%). At a median survivor follow-up of 33 months (range, 2-53), 11 patients remained on BTKi and 12 had stopped therapy because of disease progression (n = 8) or toxicity (n = 4). Our findings indicate that BTKi therapy can provide durable CLL control after disease progression on venetoclax.
RDb2C2: an improved method to identify the residue-residue pairing in β strands
Authors: Shao Di , Mao Wenzhi , Xing Yaoguang , Gong Haipeng 
Published: April 2020

AbstractBackgroundDespite the great advance of protein structure prediction, accurate prediction of the structures of mainly β proteins is still highly challenging, but could be assisted by the knowledge of residue-residue pairing in β strands. Previously, we proposed a ridge-detection-based algorithm RDb2C that adopted a multi-stage random forest framework to predict the β-β pairing given the amino acid sequence of a protein.ResultsIn this work, we developed a second version of this algorithm, RDb2C2, by employing the residual neural network to further enhance the prediction accuracy. In the benchmark test, this new algorithm improves the F1-score by > 10 percentage points, reaching impressively high values of ~ 72% and ~ 73% in the BetaSheet916 and BetaSheet1452 sets, respectively.ConclusionOur new method promotes the prediction accuracy of β-β pairing to a new level and the prediction results could better assist the structure modeling of mainly β proteins. We prepared an online server of RDb2C2 athttp://structpred.life.tsinghua.edu.cn/rdb2c2.html.
ddRADseq-assisted construction of a high-density SNP genetic map and QTL fine mapping for growth-related traits in the spotted scat (Scatophagus argus)
Authors: Yang Wei , Wang Yaorong , Jiang Dongneng , Tian Changxu , Zhu Chunhua , Li Guangli , Chen Huapu 
Published: April 2020

AbstractBackgroundScatophagus argusis a popular farmed fish in several countries of Southeast Asia, including China. AlthoughS. argushas a highly promising economic value, a significant lag of breeding research severely obstructs the sustainable development of aquaculture industry. As one of the most important economic traits, growth traits are controlled by multiple gene loci called quantitative trait loci (QTLs). It is urgently needed to launch a marker assisted selection (MAS) breeding program to improve growth and other pivotal traits. Thus a high-density genetic linkage map is necessary for the fine mapping of QTLs associated with target traits.ResultsUsing restriction site-associated DNA sequencing, 6196 single nucleotide polymorphism (SNP) markers were developed from a full-sib mapping population for genetic map construction. A total of 6193 SNPs were grouped into 24 linkage groups (LGs), and the total length reached 2191.65 cM with an average marker interval of 0.35 cM. Comparative genome mapping revealed 23 one-to-one and 1 one-to-two syntenic relationships betweenS. argusLGs andLarimichthys croceachromosomes. Based on the high-quality linkage map, a total of 44 QTLs associated with growth-related traits were identified on 11 LGs. Of which, 19 significant QTLs for body weight were detected on 9 LGs, explaining 8.8–19.6% of phenotypic variances. Within genomic regions flanking the SNP markers in QTL intervals, we predicted 15 candidate genes showing potential relationships with growth, such asHbp1,Vgll4andPim3, which merit further functional exploration.ConclusionsThe first SNP genetic map with a fine resolution of 0.35 cM forS. argushas been developed, which shows a high level of syntenic relationship withL. croceagenomes. This map can provide valuable information for future genetic, genomic and evolutionary studies. The QTLs and SNP markers significantly associated with growth-related traits will act as useful tools in gene mapping, map-based cloning and MAS breeding to speed up the genetic improvement in important traits ofS. argus. The interesting candidate genes are promising for further investigations and have the potential to provide deeper insights into growth regulation in the future.
Inactivation of the T6SS inner membrane protein DotU results in severe attenuation and decreased pathogenicity of Aeromonas veronii TH0426
Authors: Song Haichao , Kang Yuanhuan , Qian Aidong , Shan Xiaofeng , Li Ying , Zhang Lei , Zhang Haipeng , Sun Wuwen 
Published: April 2020

Abstract
Background
The inner membrane protein DotU of Aeromonas veronii is an important component of the minimal core conserved membrane proteome required for the formation of an envelope-transmembrane complex. This protein functions in a type VI secretion system (T6SS), and the role of this T6SS during the pathogenic process has not been clearly described.

Results
A recombinant A. veronii with a partial disruption of the dotU gene (720 bp of the in-frame sequence) (defined as ∆dotU) was constructed by two conjugate exchanges. We found that the mutant ∆dotU allele can be stably inherited for more than 50 generations. Inactivation of the A. veronii dotU gene resulted in no significant changes in growth or resistance to various environmental changes. However, compared with the wild-type strain colony, the mutant ∆dotU colony had a rough surface morphology. In addition, the biofilm formation ability of the mutant ∆dotU was significantly enhanced by 2.1-fold. Conversely, the deletion of the dotU gene resulted in a significant decrease in pathogenicity and infectivity compared to those of the A. veronii wild-type strain.

Conclusions
Our findings indicated that the dotU gene was an essential participant in the pathogenicity and invasiveness of A. veronii TH0426, which provides a novel perspective on the pathogenesis of TH0426 and lays the foundation for discovering potential T6SS effectors.
Phenological variation of flower longevity and duration of sex phases in a protandrous alpine plant: potential causes and fitness significance
Authors: Zhao Zhigang , Hou Meng , Wang Yijie , Du Guozhen 
Published: April 2020

Abstract
Background
Flower longevity plays an important role in pollen dispersal and reproductive success in plants. In dichogamous plants, the duration of anthesis as well as the time allocated to male and female functions can vary in response to intrinsic factors (e.g., flowering time and resource allocation) and pollination context along a growth season. However, the fitness consequences of phenological dynamics have rarely been examined. This study aims to unravel the potential causes driving variation in flower longevity, duration of sex phases, and phenotypic sex during a flowering season of strongly protandrous Aconitum gymnandrum, and particularly reproductive consequences of the phenological pattern.

Results
Population floral sex ratio shifted from completely male at the beginning to completely female at the end of the season, as is common in other protandrous plants. Phenological dynamics of the floral sex ratio and the duration of sex phases caused a shift from femaleness to maleness in the mean phenotypic sex over the whole season. Floral longevity was negatively correlated with flower size and positively affected by temperature. Early flowers within inflorescences rather than early-flowering individuals emphasized the duration of female over male phase. Owing to the dominance of male-phase flowers, early flowering for individual flowers and plants, or female-biased sex resulted in higher pollen deposition per flower and seed set. At the flower level, flower longevity positively affected female reproductive success, while the effect of flower size was negative. By contrast, plant-level female reproductive success was negatively affected by flower longevity but positively correlated to flower size.

Conclusions
The major result of this study lies in elucidating the relationship between variation in phenological sex expression and floral longevity and their fitness consequences of protandrous A. gymnandrum. The contrasting results on female fitness for individual flowers and plants contribute to our current understanding of the adaptive significance of floral longevity.
Hepatitis E virus outbreak associated with rainfall in the Central African Republic in 2008-2009
Authors: Tricou Vianney , Bouscaillou Julie , Laghoe-Nguembe Gina-Laure , Béré Aubin , Konamna Xavier , Sélékon Benjamin , Nakouné Emmanuel , Kazanji Mirdad , Komas Narcisse P. 
Published: April 2020

Abstract
Background
Infection by hepatitis E virus (HEV) can cause a high burden of morbidity and mortality in countries with poor access to clean water and sanitation. Our study aimed to investigate the situation of HEV infections in the Central African Republic (CAR).

Methods
A retrospective analysis of the blood samples and notification forms collected through the national yellow fever (YF) surveillance program, but for which a diagnosis of YF was discarded, was carried out using an anti-HEV IgM ELISA and a HEV-specific RT-PCR.

Results
Of 2883 YF-negative samples collected between January 2008 and December 2012, 745 (~ 26%) tested positive by at least either of the 2 tests used to confirm HEV cases. The results revealed that the CAR was hit by a large HEV outbreak in 2008 and 2009. The results also showed a clear seasonal pattern with correlation between HEV incidence and rainfall in Bangui. A phylogenetic analysis showed that the circulating strains belonged to genotypes 1e and 2b.

Conclusions
Overall, this study provides further evidences that HEV can be a significant cause of acute febrile jaundice, particularly among adults during rainy season or flood, in a country from Sub-Saharan Africa.
Molecular characterization of human Echinococcus isolates and the first report of E. canadensis (G6/G7) and E. multilocularis from the Punjab Province of Pakistan using sequence analysis
Authors: Khan Aisha , Ahmed Haroon , Simsek Sami , Liu Hua , Yin Jianhai , Wang Ying , Shen Yujuan , Cao Jianping 
Published: April 2020

Abstract
Background
Echinococcosis is a zoonotic parasitic disease causing serious health problems in both humans and animals in different endemic regions across the world. There are two different forms of human echinococcosis: Cystic Echinococcosis (CE) and Alveolar Echinococcosis (AE). CE is caused by the larval stage of Echinococcus granulosus sensu lato and AE by the larval stage of Echinococcus multilocularis. Geographically, CE is universally distributed, while AE is prevalent in the northern hemisphere. Although the disease is endemic in neighboring countries (China, Iran and India) of Pakistan, there are limited reports from that country. Besides, there are no comprehensive data on the genotyping of Echinococcus species in humans based on sequence analysis. This study aimed to detect the presence of human CE and to identify Echinococcus spp. in human isolates through genetic characterization of hydatid cysts in the Punjab Province of Pakistan.

Methods
Genetic analysis was performed on 38 human hydatid cyst samples collected from patients with echinococcosis using mitochondrial cytochrome c oxidase subunit 1 (cox1), cytochrome b (cytb) and NADH subunit 1 (nad1). Patient data including age, epidemiological history, sex, and location were obtained from hospital records.

Results
According to the sequence analysis we detected E. granulosus sensu stricto (n = 35), E. canadensis (G6/G7) (n = 2), and E. multilocularis (n = 1). Thus, the majority of the patients (92.1%, 35/38) were infected with E. granulosus s.s. This is the first molecular confirmation of E. canadensis (G6/G7) and E. multilocularis in human subjects from Pakistan.

Conclusions
These findings suggested that E. granulosus s.s. is the dominant species in humans in Pakistan. In addition, E. canadensis (G6/G7) and E. multilocularis are circulating in the country. Further studies are required to explore the genetic diversity in both humans and livestock.
Imaging study of coronal structural matching of the distal radius in normal adults
Authors: Zhang Xin , Yuan Feng , Yin Yong , Fan Jian 
Published: April 2020

Abstract
Background
As an important anatomical basis, coronal structural position matching of the distal radius has long been lacking in terms of a quantitative understanding, and such matching is correlated with the postoperative functional recovery of patients with distal radius fracture. The purpose of this study was to explore the degree of coronal structural matching of the distal radius in a normal population and to improve the detailed anatomical knowledge of the distal radius.

Methods
The reconstructed 3D data were analysed using 3-matic research software from thin-film CT images of 80 normal adults, and the coronal structural matching of the distal radius was studied from two aspects: 1) self-matching of the distal radius; and 2) matching between the distal radius and ulna (i.e., the joint space of the distal radioulnar joint). Specific research methods: 1) The relative position of the medial wall of the distal radius with respect to the lunate was determined as the percentage (%) of the vertical distance from the medial wall of the radius to the most prominent ulnar point of the lunate in the corresponding plane from the ulna to the radius. 2) A total of 9 sets of data were collected for evaluating the palmar lateral spacing, median spacing, and dorsal lateral spacing at the distal, middle, and proximal levels of the radius.

Results
In the study, 9 sets of data were obtained. And the data of self-matching of the coronal structural of distal radius was also obtained, was 45.0% ± 16.2%. The P values in the above data were all greater than 0.05, showing no statistical significance. Finally, data of coronal bone structural matching of distal radius in 80 normal adults were obtained.

Conclusions
Our study refines the anatomical data of the degree of coronal bone structural matching of the distal radius in a normal population. To explore the relationship between coronal alignment and function in cases of distal radius fracture, a standardized approach was established. Thin- film CT may help diagnose patients with dysplasia around the lunate and radioulnar joint that is difficult to diagnose on MRI.