bluuebunny/crossref_metadata_2025_split huggingface dataset:
3ʹS,4ʹS-Disenecioylkhellactone from the Roots of Peucedanum Japonicum Induces Apoptosis in HL-60 Cells ​
Authors: Kang Kyung-Yun , Lee Sung-Ju , Kim Hyun-Ji , Kang Heonjoong , Nam Sang-Jip , Yee Sung-Tae 
Published: December 2020

Abstract
Background: The root of Peucedanum japonicum is used in traditional medicine in Japan, the Philippines, China, and Korea, and it has been reported to have a variety of biological activities, including anticancer activity. Inducing apoptosis in tumor cells has become a major focus of antitumor drug development; therefore, we studied the apoptotic effects of the MeOH/CH2Cl2 extract of the roots of P. japonicum and its components on HL-60 cells (a human leukemia cell line).Methods: Compounds were purified using solvents with varying polarities followed by column chromatography (reverse phase), and the structures were confirmed using nuclear magnetic resonance spectroscopy. The viabilities of HL-60, A549, and MCF-7 cells were determined using the cell counting kit-8 (CCK-8) assay. Analysis of apoptosis signaling was performed only with HL-60 cells, and cell cycle progression, Annexin V/propidium iodide (PI) staining (analyzed by flow cytometry), the mitochondrial membrane potential (MMP, as analyzed by flow cytometry), and caspase-3, 8, and 9 activity (determined using the caspase-3, -8, and -9 activity kit according to the manufacturer’s protocol) were evaluated.Results: Two coumarin molecules, (-)-isosamidin (1) and 3ʹS,4ʹS disenecioylkhellactone (2), were isolated by bioactivity-guided fractionation. Only compound 2 showed cytotoxicity in HL-60 cells, which occurred due to increases in the sub-G1 population and the initiation of early and late apoptosis as determined by Annexin V/PI staining. In addition, decreases in the MMP were observed in HL-60 cells treated with compound 2. Several apoptotic features were observed, including increased cleavage of caspase-3, -8, and -9. Moreover, treatment with Z-DEVD-FMK (a caspase-3 inhibitor), Z-IETD-FMK (a caspase-8 inhibitor), or Z-LEHD-FMK (a caspase-9 inhibitor) significantly inhibited cell cytotoxicity.Conclusions: We provide evidence that compound 2 induces apoptosis in HL-60 cells.
Human Bone Marrow-Derived Mesenchymal Stem Cell-Secreted Exosomes Overexpressing Microrna-124-3p Inhibit DLBCL Progression By Downregulating&nbsp; NFATc1
Authors: Ding Xiaolei , Xu Lingzhi , Sun Xiuhua , Zhao Xiaoxuan , Gao Bing , Cheng Ye , Liu Dan , Zhao Jian , Zhang Xian , Xu Liye , Song Jin-cheng 
Published: December 2020

Abstract
Background: Exosomes play important roles in intercellular communication by delivering microRNAs (miRNAs) that mediate tumor initiation and development, including those in diffuse large B cell lymphoma (DLBCL). To date, however, limited studies on the inhibitory effect of exosomes derived from human bone marrow-derived mesenchymal stem cells (hBMSCs) on DLBCL progression have been reported. Therefore, this study aimed to investigate the role of hBMSC-secreted exosomes carrying microRNA-124-3p in the development of DLBCL.Methods: Microarray-based expression analysis was adopted to identify differentially expressed genes and regulatory miRNAs, which revealed the candidate NFATc1. Next, the binding affinity between miR-124-3p and NFATc1 was using luciferase activity assays. The mechanism underlying NFATc1 regulation was investigated using lentiviral transfections. Subsequently, DLBCL cells were cocultured with exosomes derived from hBMSCs transfected with a miR-124-3p mimic or control. Proliferation and apoptosis were measured in vitro. Finally, the effects of hBMSC-derived miR-124-3p on tumor growth were investigated in vivo.Results: MiR-124-3p was downregulated while NFATc1 was upregulated in DLBCL cells. MiR-124-3p specifically targeted and negatively regulated the expression of NFATc1 in DLBCL cells, upregulated miR-124-3p-inhibited DLBCL cell proliferation and promoted apoptosis. In addition, we found that hBMSC-derived exosomes carrying miR-124-3p repressed DLBCL cell proliferation both in vitro and in vivo.Conclusion: hBMSC-derived exosomal miR-124-3p represses the development of DLBCL through the downregulation of NFATc1.
Efficacy Evaluation Of Albumin-Bound Paclitaxel Combined With Carboplatin As Neoadjuvant Chemotherapy For Primary Epithelial Ovarian Cancer
Authors: Wang Huan , Han Yimin 
Published: December 2020

Abstract
Background: This study aimed to compare the efficacy of albumin-bound paclitaxel combined with carboplatin (Nab-TC) with that of traditional solvent-based paclitaxel combined with carboplatin (TC) as a neoadjuvant chemotherapy regimen for primary epithelial ovarian cancer. Methods: Seventy-six patients with advanced primary epithelial ovarian cancer admitted for treatment at the Third Affiliated Hospital of Harbin Medical University from January 2015 to August 2020 were retrospectively selected. All patients underwent surgery after two courses of neoadjuvant chemotherapy with a combination of paclitaxel and carboplatin. Among the patients included for study, 34 were in the Nab-TC program and 42 in the TC program. Results: After two courses of chemotherapy, the ORR value was 52.38% in the TC group and 58.82% in the Nab-TC group (P=0.355). The CA125 value of the Nab-TC group decreased by 88.28% and that of the TC group by 86.99% (P=0.358). Overall operation time, intraoperative blood loss and postoperative hospital time of patients in the Nab-TC group were lower (P>0.05) and length of total hospital stay was significantly lower (P<0.05) relative to the TC group. Moreover, incidence of bone marrow suppression, abnormal liver function, arrhythmia and hyperglycemia in the Nab-TC group were lower (P>0.05) and total incidence of nausea and vomiting as well as grade III and IV stages were significantly lower (P<0. 05) than those in the TC group. However, the frequency of acral numbness in Nab-TC group was higher compared to the TC group (P<0.05). Conclusion: The efficacy of the Nab-TC regimen as neoadjuvant chemotherapy for advanced primary epithelial ovarian cancer was equivalent to that of the TC regimen, along with a lower incidence of adverse reactions, supporting its therapeutic value in the clinic.
The Long Non-Coding RNA SNHG16 Promotes NPC Cell Progression by Competitively Binding miR-23b-3p
Authors: Wei Hou , Xu Lu , Su Tao , Wu Yunxiao , Liu Yujuan , Wei Yangao 
Published: December 2020

Abstract
Background: This study aims at verifying the effect of non-coding RNA SNHG16 on promotes NPC cell progression via binding miR-23b-3p.Methods: The expression of non-coding RNA SNHG16 was detected by qRT-PCR in cell lines including c666-1 and HONE-1. Si-MCM6 and si-SNHG16 are transfected to cells to verify their effects on cell proliferation and apoptosis. MTT is used to measure cell viability while flow cytometry assay and transwell assay were used for cell apoptosis, cell cycle and invasion respectively. The expression level of MCM6 was determined by western blot. Relationships between mRNA MCM6 and lncRNA SNHG16 were explored by qRT-PCR and nude mouse tumorigenicity assay.Results: The MCM6 was overexpressed in NPC tissues and lncRNA SNHG16 showed the same trend. Those two factors were correlated with high cancer stage. The expression of MCM6 was decreased after si-SNHG16 and dual luciferase reporter system demonstrated their combine with miR-23b-3p. Further we explored the down-regulation of lncRNA SNHG16 could inhibit NPC cell proliferation, colony formation and also accelerate cell apoptosis rate. And this result could be altered by adding miR-23b-3p inhibitor.Conclusion: The lncRNA SNHG16 is able to promote the NPC proliferation via binding miR-23b-3p, which has potential for future treatment.
Overexpression of RING Finger Protein 126 is Associated with Poor Prognosis and Contributes to the Progression of Lung Adenocarcinoma
Authors: Sun Zhaona , Chen Meiyuan , Li Ziping , Zhang Hong 
Published: December 2020

Abstract
BackgroundMore recently, E3 ubiquitin ligases are well-informed to be involved in tumor development, and genetic aberration of this family have been implicated in breast cancer and oral cancer. RING finger protein 126 (RNF126), a newly uncovered E3 ubiquitin ligase, targets multiple proteins to promote their degradation. This study aims to explore the expression pattern and functional role of RNF126 in lung adenocarcinoma (LAD). MethodsImmunohistochemical staining and real-time PCR were used to evaluate the expression pattern of RNF126 in normal lung tissue and LAD tissues. Western blot, cell proliferation and invasion assays were performed to investigate role of RNF126 in modulating LAD progression. Statistical analyses including Chi-square test, Kaplan-Meier test, Cox regression test, and Student’s t-test were conducted for both clinical and experimental data.ResultsWe find that both the mRNA and protein expression levels of RNF126 are elevated in LAD tissues, and its expression correlates with clinicopathologic features including tumor size and TNM stage. High expression of RNF126 indicates a poor prognosis of LAD patients. Gene perturbations reveal that RNF126 promotes LAD cells proliferation and xenograft growth. However, RNF126 exerts no significant effect on cell migration and invasion in LAD cells. ConclusionsOur clinical and cellular data suggest that targeting this molecule could potentially provide advantages for LAD patients with high expression of RNF126.
Genetic Diversity Analysis of Dermacentor Nuttalli within Inner Mongolia, China
Authors: Gui Zheng , Wu Lin , Cai Hao , Fu-Shaoyin  , Yu Jing Feng , Mu Lan , Yan Si-Xiao 
Published: December 2020

Abstract
BackgroundTicks (Arthropoda, Ixodida), after mosquitoes, are the next most prevalent vector of infectious diseases; and are responsible for spreading a multitude of pathogens and threatening the health and welfare of animals and human beings. Yet, given the history of tick-borne pathogen infections in the Inner Mongolia Autonomous region of China, neither the genetic diversity nor the spatial distribution of haplotypes within ticks has been studied. MethodsWe characterized the haplotype distribution of Dermacentor nuttalli in four main pastoral areas of the Inner Mongolia Autonomous region, by sampling 109 individuals (recovered from sheep) in April-August 2019. The 16S rRNA gene, Cytochrome c Oxidase Subunit Ⅰ, and the Internal Transcribed Spacer 2 region were amplified and sequenced from extracted DNA.Results 82 haplotypes were identified, the most prevalent of which was H32. 12 sequences (11 of all sequences) represented the most abundant haplotypes, with a highly scattered distribution. Total haplotype diversity was 0.98318, while total nucleotide diversity was 0.11369. Neutrality tests revealed negative results in the four locations analyzed, which is indicative of an excess of recently derived haplotypes. Fixation index values (FST) indicate that the degree of genetic differentiation amongst some sampled populations were small, while others were moderate. Conclusion The genetic diversity of D.nuttalli populations in our region can likely adapt to different geographical environments, thereby leading to genetic diversity, and creating genetic differentiation amongst different populations. However, genetic differentiation is cryptic and does not form the pedigree geographical structure.
The Effects of the Field Cold Stress in the Harvest Period on the Quality and Curing Characteristics of Tobacco Leaves
Authors: Li Yan , Ren Ke , Hu Mengyang , He Xian , Gu Kaiyuan , Hu Binbin , Su Jiaen , Jin Yan , Gao Wenyou , Yang Daosheng , Li Folin , Zou Congming 
Published: December 2020

Abstract
Background: Climate change in high-altitude areas causes tobacco (Nicotiana tabacum L.) in its mature period to be subjected to cold stress, which damages the yield and quality of tobacco leaves. We found that abrupt diurnal temperature difference (the daily temperature drops more than 20℃), along with rainfall occurred in the tobacco-growing areas at an altitude above 2450 m, which caused cold stress to tobacco in field. Results: The change process of the surface color of tobacco leaves was normal to dark green (within 1 to 2 d), to purple red (within 2 to 3 d), then dark red (within 3 to 4 d), and finally off-white (within 4 to 6 d), the final leaves appear obvious large areas browning, and the curing availability was extremely poor. Further research found the quality of fresh tobacco leaves, the content of key chemical components, and the quality of production are greatly reduced by cold stress. The reason was cold stress in high altitude environment destroyed the antioxidant enzyme system of mature flue-cured tobacco. Cold stress in high altitude environment destroyed the antioxidant enzyme system of mature flue-cured tobacco. The quality of fresh tobacco leaves, the content of key chemical components, and the quality of production are greatly reduced by cold stress.Conclusion: In conclusion, this study confirmed that the field cold stress in high-altitude tobacco areas was the main reason for the obvious browning tobacco leaves during the tobacco curing process. This adversity environment seriously damaged the quality of tobacco leaves.
Circulating Omentin-1, Insulin Resistance and Lipid Profile Responses Following an Eight-week Aerobic Training Intervention Among Male Smokers&nbsp;
Authors: Mousavi Seyed Maysam , Heidarianpour Ali , Tavassoli Hassan 
Published: December 2020

Abstract
Background: Omentin-1 is a recently discovered circulating adipokine that plays a crucial role in modulating insulin resistance and diabetes. We investigated the effect of eight weeks of aerobic exercise training on serum omentin-1, insulin resistance and lipid profile in the smokers and non-smokers with normal weight. Methods: Nineteen male healthy and twenty male smokers were randomly assigned into healthy control group (C), healthy exercise group (E), control smoker group (CS) and exercise smoker group (ES). Exercise groups participated in an eight-week aerobic exercise training program (three times a week, 20-35 min per session at 55%-70% of maximum heart rate). Serum omentin-1 and insulin values were determined by ELISA and Homeostatic Model Assessment for Insulin Resistance (HOMA-IR), glucose and lipid profile was measured before and after the intervention. Paired samples t-test, one-way analysis of variance (One-way ANOVA) and post-hoc Tukey test were applied to analyze the data (p<0.05).Results: Aerobic exercise improved both serum omentin-1 and high lipoprotein cholesterol (HDL-C) in the exercise groups (p<0.05). Also, Exercise training reduced insulin, blood sugar, HOMA-IR, total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) levels (p<0.05). Omentin-1 was significantly correlated with insulin (r=-0.40, P=0.01), HOMA-IR (r=-0.38, P=0.04), TG (r=-0.40, P=0.01), TC (r=-0.49, P=0.02), LDL-C (r=-0.70, P=0.02) and HDL-C (r=0.55, P=0.03).Conclusion: The findings suggest that aerobic exercise-induced changes in omentin-1 in exercise trained smokers may be associated with the beneficial effects of exercise on reduced insulin resistance and lipid profile.
Survival Status and Determinants of Mortality Among Severely Malnourished Children Aged 0-59 Months Admitted to Jinka Hospital, South Omo Zone, Ethiopia: A Retrospective Cohort Study&nbsp;
Authors: Abiyu Shegaw , Gobena Tesfaye , Egata Gudina 
Published: December 2020

Abstract
Sever acute malnutrition (SAM) remains a major public health problem contributing to morbidity and mortality among children aged 0-59 months. However, little is known about the survival status and determinants of mortality among children admitted to stabilization centers (SC) in Ethiopia particularly in study setting. The objective: to assess the survival status and determinants of mortality among severely malnourished children aged 0-59 months admitted to SC in Jinka general hospital from September 1, 2014 to August 30, 2018. Hospital based retrospective cohort study design was used among 588 severely malnourished children aged 0-59 months admitted to SC of Jinka general hospital from March 20-30, 2019. Simple random sampling method was used to select medical records of the study participants. Proportional hazard assumption over time was checked by Schoenfeld’s global test. The Kaplan-Mei­er survival curve with log-rank test was used to compare survival across groups. Bivariable and multivariable Cox proportional hazard regression was used to assess the association between each independent variable and the outcome variable. Level of statistical significance was declared at p less than 0.05. Among cohort of children studied, nearly 11% of children were died during follow up. The overall mean survival time was 38 days, 95% CI :( 36.3, 39.7).Urban residence [AHR=2.24, 95% CI: (1.17, 4.30)], presence of dehydration [AHR =3.94, 95% CI:(1.89, 8.22)], presence of shock [AHR=4.15, 95% CI:(2.01, 8.55)], altered body temperature [AHR= 2.01, 95% CI:(1.01, 3.91)], failure to take F100 formula milk [AHR= 4.87, 95%CI:(2.75, 8.63)] and oral antibiotics [AHR=3.57, 95%CI:(1.88,6.77)] were identified to be the independent determinants of mortality The mortality rate for severely undernourished children was higher than global sphere standard and national protocol.Societal and individual level determinants of mortality were identified. Therefore, interventions to reduce mortality should focus on altered clinical conditions and comorbidities/ infections.
Identification of 11 candidate structured noncoding RNA motifs in humans by comparative genomics
Authors: Hou Lijuan , Xie Jin , Wu Yaoyao , Wang Jiaojiao , Duan Anqi , Ao Yaqi , Liu Xuejiao , Yu Xinmei , Yan Hui , Perreault Jonathan , LI SANSHU 
Published: December 2020

Abstract
Background
Only 1.5% of the human genome encodes proteins, while most of the remaining encodes noncoding RNAs (ncRNA). Many ncRNAs form structures and perform many important functions. Accurately identifying structured ncRNAs in the human genome and discovering their biological functions remain a major challenge.
Results
Here, we have established a pipeline (CM-line) with the following features for analyzing the large genomes of humans and other animals. First, we selected species with larger genetic distances to facilitate the discovery of covariations and compatible mutations. Second, we used CMfinder, which can generate useful alignments even with low sequence conservation. Third, we removed repetitive sequences and known structured ncRNAs to reduce the workload of CMfinder. Fourth, we used Infernal to find more representatives and refine the structure. We reported 11 classes of structured ncRNA candidates with significant covariations in humans. Functional analysis showed that these ncRNAs have variable functions. Some may regulate circadian clock genes through poly (A) signals (PAS); some may regulate the elongation factor (EEF1A) and the T-cell receptor signaling pathway by cooperating with RNA binding proteins.
Conclusions
By searching for important features of RNA structure from large genomes, the CM-line has revealed the existence of a variety of novel structured ncRNAs. Functional analysis provides evidence for the potential biological functions of some newly found ncRNA motifs. The pipeline we have established for the discovery of structured ncRNAs and the identification of their functions can also be applied to analyze other large genomes.
Plastid phylogenomic insights into the evolution of Distylium (Hamamelidaceae)
Authors: Dong Wenpan , Liu Yanlei , Xu Chao , Gao Yongwei , Zhang Zhixiang , Yuan Qingjun , Suo Zhili , Sun Jiahui 
Published: December 2020

Abstract
Background: Most Distylium species are endangered. Distylium species mostly display homoplasy in their flowers and fruits, and are classified primarily based on leaf morphology. However, leaf size, shape, and serration vary tremendously making it difficult to use those characters to identify most species and a significant challenge to address the taxonomy of Distylium. To infer robust relationships and identify variable markers to identify Distylium species, we sequenced most of the Distylium species chloroplast genome. Results: The Distylium chloroplast genome size was 159,041–159,127 bp and encoded 80 protein-coding, 30 transfer RNAs, and 4 ribosomal RNA genes. There was a conserved gene order displayed and a typical quadripartite structure. Phylogenomic analysis based on whole chloroplast genome sequences yielded a highly resolved phylogenetic tree and formed a monophyletic group containing four Distylium clades. A dating analysis suggested that Distylium originated in the Oligocene (34.39 Ma) and diversified within approximately 1 Ma. The evidence shows that Distylium is a rapidly radiating group. Four highly variable markers, such as matK-trnK, ndhC-trnV, ycf1, and trnT-trnL, and 74 polymorphic simple sequence repeats were discovered in the Distylium plastomes. Conclusions: The plastome sequences had sufficient polymorphic information to resolve phylogenetic relationships and identify species accurately.
Comparative Transcriptome Analysis Revealed Candidate Genes Potentially Related to Desiccation Sensitivity of Recalcitrant Quercus variabilis Seeds
Authors: Li Dongxing , Li Yingchao , Qian Jialian , Liu Xiaojuan , Xu Huihui , Zhang Guowei , Ren Junjie , Wang Libing , Zhang Lu , Yu Haiyan 
Published: December 2020

Abstract
Background: Chinese cork oak (Quercus variabilis) is a widely distributed and highly valuable deciduous broadleaf tree from both ecological and economic perspectives. Seeds of Q. variabilis are recalcitrant, i.e., sensitive to desiccation, which affects their storage and long-term preservation of germplasm. However, little is known about the underlying mechanism of desiccation sensitivity of Q. variabilis seeds. Results: In this study, the seeds were desiccated with silica gel for 0 day (control, CK), one day (T1) to 15 days (T15). After desiccation, the transcriptomic profiles of these different desiccation stages were compared using the Quercus suber genome as a reference, as well four key stages (CK, T2, T4 and T11) of desiccation sensitivity of Q. variabilis seeds through germination test were identified. A total of 4405, 4441, and 5907 differentially expressed genes (DEGs) were identified in T2 vs CK, T4 vs CK, and T11 vs CK, respectively. Among them, 2219 DEGs were overlapped in the three comparison groups. KEGG (Kyoto Encyclopaedia of Genes and Genomes) enrichment analysis showed that these DEGs were enriched into 124 pathways, such as "plant hormone signal transduction" and "glycerophospholipid metabolism". DEGs related to hormone synthesis and signal transduction (ZEP, YUC, PYR, ABI5, ERF1B, etc), stress response proteins (LEA D-29, HSP70, etc), and phospholipase D (PLD1) were detected during seed desiccation. These genes and their interactions may regulate the desiccation sensitivity of Q. variabilis seeds. Finally, a possible work model was proposed to show the molecular regulation mechanism of desiccation sensitivity in recalcitrant Q. variabilis seedsConclusions: Our study is the first on the molecular regulation mechanism of desiccation sensitivity of Q. variabilis seeds by using RNA-Seq and propose a possible work model. Our findings could make a great contribute to seed storage and long-term conservation of germplasm resources of recalcitrant seeds in the future.