bluuebunny/crossref_metadata_2025_split huggingface dataset:
Photon mass limits driven by axions, Lorentz violation, chiral and gravitational anomalies in non-Riemannian spaces, and helical dynamo seeds
Authors: Garcia de Andrade L.C. 
Published: August 2020

In this paper two types of applications of Cartan torsion to cosmology and quantum field theory are given. The first is given by massive photon axial torsion mixing through non-minimal coupling. It is shown that chiral anomalies induce upper limits on the photon mass and galactic dynamo seeds. In this example we obtain magnetic field seeds of the order of 10−16 G from a strong chiral battery magnetic field of the order of 1030 G. This cosmic magnetic field is in accordance with previous magnetic dynamo seed limits as 10−22 G ≤ Bseed ≤ 10−12 G given by Barrow et al. (Phys. Rev. D, 86, 023533 (2012). doi: 10.1103/PhysRevD.86.023533 ). In this example, as in the Prokopec et al. (Phys. Rev. Lett. 89, 101301 (2002). doi: 10.1103/PhysRevLett.89.101301 ) torsionless case, which associates Dolgov anomalies to photon mass limits, we show that at early universe scales, 10−32 eV is a very stringent limit. Here we can show that the parameter [Formula: see text], which is well within the lower bound of r needed for the magnetic field to seed galactic dynamos. Helical magnetic fields are obtained. One can also derive massive photon limits from axial chiral anomalies with torsion degrees of freedom (Duncan et al. Nucl. Phys. B, 387, 215 (1992). doi: 10.1016/0550-3213(92)90052-D ) leading to photon mass of the order of 10−11 eV. Dynamo equations are obtained from anomalous Maxwell equations as well as magnetic wave equations with photon mass, which are analytically solved. In the second example one shows that Chern–Simons (CS) current is affected by the presence of minimal coupled torsion. In this case as well one obtains a photon mass limit of the order 10−18 eV. Gravitational anomalies in Riemann–Cartan spacetime with axial torsion are also obtained. Recently, Terrano et al. (Phys. Rev. Lett. 122, 231301 (2019). doi: 10.1103/PhysRevLett.122.231301 ) computed dark matter mass constraints of the order of 10−23 eV from spin-polarised torsion balances, which has given some support for torsion theories of Einstein–Cartan type. Here, however, we deal with torsion with dynamical degrees of freedom. Lorentz violation in non-Riemannian CS currents are investigated.
Inclusive probability of particle creation on classical backgrounds
Authors: Kazinski Peter 
Published: August 2020

AbstractThe quantum theories of boson and fermion fields with quadratic nonstationary Hamiltoanians are rigorously constructed. The representation of the algebra of observables is given by the Hamiltonian diagonalization procedure. The sufficient conditions for the existence of unitary dynamics at finite times are formulated and the explicit formula for the matrix elements of the evolution operator is derived. In particular, this gives the well-defined expression for the one-loop effective action. The ultraviolet and infrared divergencies are regularized by the energy cutoff in the Hamiltonian of the theory. The possible infinite particle production is regulated by the corresponding counterdiabatic terms. The explicit formulas for the average number of particles$$N_D$$NDrecorded by the detector and for the probabilityw(D) to record a particle by the detector are derived. It is proved that these quantities allow for no-regularization limit and, in this limit,$$N_D$$NDis finite and$$w(D)\in [0,1)$$w(D)∈[0,1). As an example, the theory of a neutral boson field with stationary quadratic part of the Hamiltonian and nonstationary source is considered. The average number of particles produced by this source from the vacuum during a finite time evolution and the inclusive probability to record a created particle are obtained. The infrared and ultraviolet asymptotics of the average density of created particles are derived. As a particular case, quantum electrodynamics with a classical current is considered. The ultraviolet and infrared asymptotics of the average number of photons are derived. The asymptotics of the average number of photons produced by the adiabatically driven current is found.
Numerical calculation of the quasinormal frequencies for the Dirac field in a Lifshitz black brane
Authors: Ares de Parga-Regalado A. M. , López-Ortega A. 
Published: August 2020

AbstractIn the zero momentum limit we numerically calculate the quasinormal frequencies of the massive Dirac field propagating in a Lifshitz black brane. We focus on the non-exactly solvable cases for the fermionic perturbations, so that our results are an extension of the examples already reported for the massive Klein–Gordon and Dirac fields in the zero momentum limit. Based on our numerical results, we propose an analytical approximation of the obtained quasinormal frequencies of the Dirac field and compare their behavior with those of the Klein–Gordon field. We extend the results on the Klein–Gordon quasinormal frequencies already published. Furthermore, by imposing the Dirichlet boundary condition at the asymptotic region, we are able to find more general results for the fermionic exactly solvable case previously studied.
Abstract IA25: Molecular biomarkers of chemoradiation response in bladder preservation therapy
Authors: Miyamoto David T. 
Published: August 2020

Abstract
The standard definitive treatment options for muscle-invasive bladder cancer are radical cystectomy or bladder-sparing trimodality therapy (TMT), consisting of maximal transurethral resection of bladder tumor followed by chemoradiation therapy. Although multiple series show these two treatment approaches have similar overall long-term outcomes, recent studies suggest that molecular biomarkers may identify subgroups of patients who may preferentially benefit from one type of therapy over the other. Such biomarkers include tumor-intrinsic genes involved in DNA repair and signal transduction, which confer sensitivity or resistance to radiation therapy based on the intrinsic biology of the tumor. Emerging biomarkers also include molecular signatures of the tumor microenvironment, suggesting an important role for tumor-extrinsic factors in governing therapeutic sensitivity or resistance. This presentation will review the evidence and rationale underlying potential prognostic and predictive molecular biomarkers in bladder preservation therapy. A specific focus will be on our recent gene expression profiling of 136 muscle-invasive bladder cancer patients treated with bladder-sparing TMT. Our analysis showed that higher immune infiltration was associated with improved disease-specific survival after TMT, whereas higher stromal infiltration was associated with shorter disease-specific survival in a comparison cohort treated with radical cystectomy after neoadjuvant chemotherapy. These findings require validation studies in additional cohorts, which are currently ongoing. An additional focus of the presentation will be on emerging liquid biopsy biomarkers of minimal residual disease after bladder cancer therapy. The standard follow-up for patients after bladder-sparing TMT includes cystoscopies with biopsies and imaging studies at regular intervals. Liquid biopsy approaches such as circulating tumor cells (CTCs) and circulating tumor DNA (ctDNA) may enable noninvasive monitoring of response and detection of minimal residual disease after therapy, with a substantial lead time over conventional follow-up approaches including imaging. If validated in prospective clinical trials, molecular biomarkers may guide the tailored selection of appropriate treatments for individual patients with muscle-invasive bladder cancer, as well as enable noninvasive monitoring of patients after therapy. Such biomarkers also have potential to guide the use of synergistic combinations of immunotherapy with chemoradiation therapy for bladder cancer.
Citation Format: David T. Miyamoto. Molecular biomarkers of chemoradiation response in bladder preservation therapy [abstract]. In: Proceedings of the AACR Special Conference on Bladder Cancer: Transforming the Field; 2019 May 18-21; Denver, CO. Philadelphia (PA): AACR; Clin Cancer Res 2020;26(15_Suppl):Abstract nr IA25.
Antiplatelet Effect of Carbon Monoxide Is Mediated by NAD
+
and ATP Depletion
Authors: Kaczara Patrycja , Sitek Barbara , Przyborowski Kamil , Kurpinska Anna , Kus Kamil , Stojak Marta , Chlopicki Stefan 
Published: August 2020

Objectives:
Carbon monoxide (CO) produced by haem oxygenases or released by CO-releasing molecules (CORM) affords antiplatelet effects, but the mechanism involved has not been defined. Here, we tested the hypothesis that CO–induced inhibition of human platelet aggregation is mediated by modulation of platelet bioenergetics.

Approach and Results:

To analyze the effects of CORM-A1 on human platelet aggregation and bioenergetics, a light transmission aggregometry, Seahorse XFe technique and liquid chromatography tandem-mass spectrometry–based metabolomics were used. CORM-A1–induced inhibition of platelet aggregation was accompanied by the inhibition of mitochondrial respiration and glycolysis. Interestingly, specific inhibitors of these processes applied individually, in contrast to combined treatment, did not inhibit platelet aggregation considerably. A CORM-A1–induced delay of tricarboxylic acid cycle was associated with oxidized nicotinamide adenine dinucleotide (NAD
+
) depletion, compatible with the inhibition of oxidative phosphorylation. CORM-A1 provoked an increase in concentrations of proximal (before GAPDH [glyceraldehyde 3-phosphate dehydrogenase]), but not distal glycolysis metabolites, suggesting that CO delayed glycolysis at the level of NAD
+
–dependent GAPDH; however, GAPDH activity was directly not inhibited. In the presence of exogenous pyruvate, CORM-A1–induced inhibition of platelet aggregation and glycolysis were lost, but were restored by the inhibition of lactate dehydrogenase, involved in cytosolic NAD
+
regeneration, pointing out to the key role of NAD
+
depletion in the inhibition of platelet bioenergetics by CORM-A1.

Conclusions:

The antiplatelet effect of CO is mediated by inhibition of mitochondrial respiration—attributed to the inhibition of cytochrome c oxidase, and inhibition of glycolysis—ascribed to cytosolic NAD
+
depletion.
Cholesterol Efflux-Independent Modification of Lipid Rafts by AIBP (Apolipoprotein A-I Binding Protein)
Authors: Low Hann , Mukhamedova Nigora , Capettini Luciano dos Santos Aggum , Xia Yining , Carmichael Irena , Cody Stephen H. , Huynh Kevin , Ditiatkovski Michael , Ohkawa Ryunosuke , Bukrinsky Michael , Meikle Peter J. , Choi Soo-Ho , Field Seth , Miller Yury I. , Sviridov Dmitri 
Published: August 2020

Objective:
AIBP (apolipoprotein A-I binding protein) is an effective and selective regulator of lipid rafts modulating many metabolic pathways originating from the rafts, including inflammation. The mechanism of action was suggested to involve stimulation by AIBP of cholesterol efflux, depleting rafts of cholesterol, which is essential for lipid raft integrity. Here we describe a different mechanism contributing to the regulation of lipid rafts by AIBP.

Approach and Results:
We demonstrate that modulation of rafts by AIBP may not exclusively depend on the rate of cholesterol efflux or presence of the key regulator of the efflux, ABCA1 (ATP-binding cassette transporter A-I). AIBP interacted with phosphatidylinositol 3-phosphate, which was associated with increased abundance and activation of Cdc42 and rearrangement of the actin cytoskeleton. Cytoskeleton rearrangement was accompanied with reduction of the abundance of lipid rafts, without significant changes in the lipid composition of the rafts. The interaction of AIBP with phosphatidylinositol 3-phosphate was blocked by AIBP substrate, NADPH (nicotinamide adenine dinucleotide phosphate), and both NADPH and silencing of Cdc42 interfered with the ability of AIBP to regulate lipid rafts and cholesterol efflux.

Conclusions:
Our findings indicate that an underlying mechanism of regulation of lipid rafts by AIBP involves PIP-dependent rearrangement of the cytoskeleton.
Predictors of Mortality in Patients With Biopsy‐Proven Viral Myocarditis: 10‐Year Outcome Data
Authors: Greulich Simon , Seitz Andreas , Müller Karin A. L. , Grün Stefan , Ong Peter , Ebadi Nawid , Kreisselmeier Klaus Peter , Seizer Peter , Bekeredjian Raffi , Zwadlo Carolin , Gräni Christoph , Klingel Karin , Gawaz Meinrad , Sechtem Udo , Mahrholdt Heiko 
Published: August 2020

Background
There is scarce data about the long‐term mortality as well as the prognostic value of cardiovascular magnetic resonance and late gadolinium enhancement (LGE) in patients with biopsy‐proven viral myocarditis. We sought to investigate: (1) mortality and (2) prognostic value of LGEcardiovascular magnetic resonance (location, pattern, extent, and distribution) in a >10‐year follow‐up in patients with biopsy‐proven myocarditis.

Methods and Results

Two‐hundred three consecutive patients with biopsy‐proven viral myocarditis and cardiovascular magnetic resonance were enrolled; 183 patients were eligible for standardized follow‐up. The median follow‐up was 10.1 years. End points were all‐cause death, cardiac death, and sudden cardiac death (SCD). We found substantial long‐term mortality in patients with biopsy‐proven myocarditis (39.3% all cause, 27.3% cardiac, and 10.9% SCD); 101 patients (55.2%) demonstrated LGE. The presence of LGE was associated with a more than a doubled risk of death (hazard ratio [HR], 2.40; 95% CI], 1.30–4.43), escalating to a HR of 3.00 (95% CI, 1.41–6.42) for cardiac death, and a HR of 14.79 (95% CI, 1.95–112.00) for SCD; all
P
≤0.009. Specifically, midwall, (antero‐) septal LGE, and extent of LGE were highly associated with death, all
P
<0.001. Septal LGE was the best independent predictor for SCD (HR, 4.59; 95% CI, 1.38–15.24;
P
=0.01).

Conclusions
In patients with biopsy‐proven viral myocarditis, the presence of midwall LGE in the (antero‐) septal segments is associated with a higher rate of mortality (including SCD) compared with absent LGE or other LGE patterns, underlining the prognostic benefit of a distinct LGE analysis in these patients.
Salmonellosis Outbreak After a Large-Scale Food Event in Virginia, 2017
Authors: Shaw Kelly A. , Wright Kimberly , Privett Keith , Holloman Kelsey , Levine Seth , McCombs Katherine , Turner Lauren , Holsinger Caroline , Woolard Diane 
Published: August 2020

Objectives The Virginia Department of Health (VDH) identified an outbreak of Salmonella serotype Javiana infections in Virginia after a chili and chowder cook-off with 11 competitors and about 2500 attendees on September 30, 2017. The objectives of this study were to assess the extent of the outbreak and identify the most likely source of exposure. Methods To identify people with suspected Salmonella infection, VDH used press releases and social media posts to recruit event attendees to take an online survey about foods eaten at the cook-off and any gastrointestinal (GI) symptoms experienced. VDH defined a case as reported GI illness that occurred within 1 week after eating food from the cook-off. Confirmed cases required a clinical specimen culture positive for Salmonella. Probable cases reported diarrhea or related clinical symptoms. “Not ill” people did not report GI illness. Investigators calculated unadjusted relative risks of illness and performed stratified analysis to address potential confounding. Available food samples were tested for Salmonella. Environmental health specialists interviewed food handlers and inspected restaurants where 3 competitors had prepared food. Results Of 438 survey responses, 171 (39%) met the case definition. Of all chilies and chowders consumed, Chowder A was associated with the highest relative risk of illness (8.9; 95% CI, 5.7-13.7). A Chowder A sample tested positive for Salmonella serotype Javiana. The environmental investigation did not identify an original contamination source but did find deficiencies in maintaining safe cooking temperatures. Conclusions Epidemiologic and laboratory findings indicated that Chowder A was the most likely cause of outbreak. Recommendations to prevent future outbreaks include preparation of all food on-site to ensure safe temperatures during food preparation and service.
Aerosol-generating procedures in thoracic surgery in the COVID-19 era in Malaysia
Authors: Sathiamurthy Narasimman , Balasubbiah Narendran , Dharmaraj Benedict 
Published: August 2020

Background The Covid-19 pandemic has caused changes in the surgical treatment of non-Covid patients, especially in thoracic surgery because most procedures are aerosol generating. Hospital Kuala Lumpur, where thoracic procedures are performed, was badly affected. We describe our experience in performing aerosol generating procedures safely in thoracic surgery during the Covid-19 era. Methods Medical records of patients who underwent thoracic surgery from March 18, 2020 to May 17, 2020 were reviewed retrospectively. All patients undergoing thoracic surgery were tested for Covid-19 using the reverse transcriptase polymerase chain reaction method. Patients with malignancy were observed for 10 to 14 days in the ward after testing negative. The healthcare workers donned personal protective equipment for all the cases, and the number of healthcare workers in the operating room was limited to the minimum required. Results A total of 44 procedures were performed in 26 thoracic surgeries. All of these procedures were classified as aerosol generating, and the mean duration of the surgery was 130 ± 43 minutes. None of the healthcare workers involved in the surgery were exposed or infected by Covid-19. Conclusion Covid-19 will be a threat for a long time and thoracic surgeons must continue to provide their services, despite having to deal with aerosol generating procedures, in the new normal. Covid-19 testing of all surgical candidates, using the reverse transcriptase polymerase chain reaction, donning full personal protective equipment for healthcare workers, and carefully planned procedures are among the measures suggested to prevent unnecessary Covid-19 exposure in thoracic surgery.
Does Morphine Affect Astrocytes?
Authors: Rönnbäck Lars , Hansson Elisabeth 
Published: August 2020

Astrocytes in primary culture express receptors for monoamine and peptide neurotransmitters. The cells also exhibit uptake sites for amino acid neurotransmitters. New data is presented, demonstrating interactions between the second messenger systems for vasoactive intestinal peptide (VIP) and α- and β-adrenoceptors. Stimulation of these monoamine receptors modulates GABA and glutamate uptake into the cells, which indicates that astrocytes have the capacity to supervise and regulate synaptic transmission. Morphine affects astrocytes directly, probably by interaction with α2 receptors, and indirectly by drug-induced ionic changes in the synaptic region, followed by changes in neurotransmission and protein synthesis. Modulation of astroglial specific functions or metabolism might be one way to affect functional neuronal disturbances, including an approach toward understanding the underlying cellular mechanisms, e.g. tolerance.
2,4-Dichlorophenol and MCPA in a V79 Test
Authors: Fiskesjö Geirid 
Published: August 2020

Two industrial chemicals, 2,4-dichlorophenol and 4-chloro-2-methylphenoxyacetic acid (MCPA), which have no toxic effects on the Chinese hamster cell line V79 alone, were tested for toxicity and mutagenicity in a cell-mediated test, where mixed-function oxidase (MFO) enzymes are active in the metabolism of xenobiotics. For 2,4-dichlorophenol, a dose-dependent toxicity as well as a slight mutagenicity could be shown when oxygenation enzymes were present. A similar degree of toxicity in a plant test system (the Allium test) indicates a similar risk of damage from exposure to dichlorophenol treatments in both these systems. MCPA did not induce any toxic or mutagenic effects at the concentrations tested. These results were not in agreement with previous results in plant material, where MCPA was clearly toxic at relatively low doses. However, since chlorophenols have been found in plants sprayed with phenoxyacetic acids, further investigations should be performed concerning potential risk to human beings.
Animal Experiments and their Alternatives in Psychiatry
Authors: Stevens Anthony 
Published: August 2020

During the last twenty years, the most enthusiastic advocates of the use of animal models in the study of human psychiatric dysfunction have been Harlow and Suomi. In an influential paper, Induced Depression in Monkeys (1974), they argued that more extensive use of non-human primates “would have great potential utility since many manipulations and measurements presently prohibited in human study by ethical and practical considerations could be readily performed on non-human primate subjects in well-controlled experimental environments.” Harlow & Suomi concluded this paper with the following statement: “The results obtained to date on induced depression in monkeys show that proper and profound depressions can be produced relatively easily by a variety of techniques. These induced depressions either bear a close resemblance to human depression or have such similarity as to suggest that closely correlated human and animal depressive patterns may be achieved with refined techniques. The results to date also provide adequate data for the conduct of meaningful researches on the effects of pharmacological agents which either enhance, inhibit or preclude the experimental production of depression. Further, the existence of firm and fast monkey depression syndromes offers vast opportunities for testing a wide range of therapeutic techniques, either behavioural or biochemical.”
The Hep G2 Cell Line as a Possible Alternative to Isolated Hepatocytes in Cytotoxicity Studies
Authors: Marinovich Marina , Lorenzo Jose L. , Flaminio Liliana M. , Granata Agnese , Galli Corrado L. 
Published: August 2020

The hepatotoxicity of carbon tetrachloride (CC14) was evaluated in vitro in freshly isolated rat hepatocytes and in the human hepatoma cell line, Hep G2. Toxicity was assessed by the leakage of cytosolic enzymes (lactate dehydrogenase and aspartate aminotransferase) and cell viability (trypan blue exclusion). The established human cells were less sensitive to CCl4-induced injury; higher doses of the toxic agent and longer incubation times were necessary to elicit cell damage. Micromolar concentrations of prostaglandin E2 significantly decreased enzyme leakage in both Hep G2 cells and rat hepatocytes challenged with CC14; a stable derivative of prostacyclin (ZK 36374) was ineffective. These results suggest that human hepatoma Hep G2 cells may represent a valid alternative to isolated rat hepatocytes for an initial approach to the in vitro evaluation of cell toxicity.