bluuebunny/crossref_metadata_2025_split huggingface dataset:
Oxidative Stress and Endothelial Dysfunction in the Development of Simulated Radio-Induced Lung Damage and their Correction with Pulmonary Surfactant
Authors: Kirillov Yu. A. , Chernov I. A. , Malysheva E. M. , Timofeev S. E. , Kukushkin V. I. , Zharkov N. V. , Rozenberg O. A. 
Published: April 2020

Radiation exposure to the chest organs used in the complex treatment of neoplasms is often accompanied by the development of radiation pneumonitis, fibrosis and respiratory failure.The aim of the study was to evaluate oxidative stress and endothelial dysfunction in radio-induced lung damage and possibility of their correction with a pulmonary surfactant preparation.Material and methods. Lung tissue samples of 82 male Wistar rats were investigated in the study. Single local irradiation of animals was performed with the X-ray RUM-17 unit at a dose 12 Gy. The quantitative content and fractional composition of phospholipids of pulmonary surfactant were determined in the study. Oxidative stress was detected using Raman spectroscopy, endothelial dysfunction was detected immunohistochemically.Results. Oxidative stress and endothelial dysfunction were registered starting from the first day of the experiment, and manifested by an increased content of free radicals and a decrease in endothelial function indices supported by precise spectroscopic, immunohistochemical and electron microscopic methods. With application of Surfactant-BL preparation oxidative stress manifestations were eliminated to the beginning of the intermediate stage of the experiment, and were preserved without correction until its completion.Conclusions. Three-fold intratracheal administration of a pulmonary surfactant preparation implements an antioxidant, immunomodulatory and compensating effect attenuating the alterative effect of oxidative stress and its initiating impact on the development of endothelial dysfunction. Native surfactant having a pronounced effect on all components of the lung tissue including alveolocytes of the first and second types and endothelial cells of the capillaries of the lung results in the correction of endothelial dysfunction.
Guías de Práctica Clínica para la prevención, detección temprana y tratamiento de las complicaciones del embarazo, parto o puerperio: introducción y metodología
Authors: Gómez-Sánchez Pío Iván , Arévalo-Rodríguez Ingrid , Rubio-Romero Jorge Andrés , Amaya-Guío Jairo , Osorio-Castaño John Henry , Buitrago-Gutiérrez Giancarlo , Desarrollador de la Guía Representantes Grupo 
Published: April 2020

Antecedentes: las “Guías de Práctica Clínica (GPC) para la prevención, detección temprana y tratamiento de las alteraciones del embarazo, parto y el puerperio” fueron desarrolladas por la alianza conformada por la Universidad Nacional de Colombia, la Universidad de Antioquia, la Universidad Pontificia Javeriana y el Centro de investigaciones en evaluación de tecnologías en salud (CINETS) dentro de la convocatoria del Ministerio de Salud y Protección Social y Colciencias en el año 2010 para el desarrollo de GPC en temas prioritarios de salud.Materiales y métodos: basados en la “Guía Metodológica para la elaboración de GPC” del Centro de Estudios e Investigación en Salud (CEIS), se conformó el grupo desarrollador, se definieron los tópicos por desarrollar, el alcance, los objetivos y las preguntas por resolver en las GPC. Se realizó una búsqueda sistemática de GPC para los tópicos seleccionados, priorizando la opción de adaptar antes que desarrollar una guía de novo, según la calidad metodológica de las guías identificadas. Se realizó la revisión sistemática de la literatura para responder preguntas huérfanas. Se formularon las recomendaciones con niveles de evidencia graduados bajo el sistema SIGN. Se favoreció la participación de pacientes y de los diferentes grupos de interés.Resultados: se adaptaron seis GPC para responder las preguntas de los tópicos incluidos en la GPC desarrollada. El tópico de toxoplasmosis requirió el desarrollo de recomendaciones de novo.Conclusiones: estas GPC fueron elaboradas con procedimientos sistemáticos y la mejor evidencia disponible. Se espera que contribuya a mejorar la calidad de la atención de las gestantes y a la disminución de la morbilidad y mortalidad materna y perinatal.
Stratigraphical reinterpretation of Devonian strata underlying the Mons Basin based on cuttings from the Saint-Ghislain borehole, Hainaut, Belgium
Authors: LICOUR Luciane , GODERNIAUX Pascal , DUPONT Nicolas , HENNEBERT Michel , SWENNEN Rudy , STEEMANS Philippe , BOULVAIN FRÉDÉRIC , PETITCLERC Estelle , RORIVE Alain , BAELE Jean-Marc 
Published: April 2020

It is revealed that the lowest 1010 m of the Saint-Ghislain borehole (-4393 to -5403 m), of which cuttings are available, constitutes a crucial source of information to investigate, amongst others, the deep geothermal potential within the Brabant Parautochthon, underlying the Mons Basin, Hainaut. The lithological succession of this interval was reconstructed based mainly on visual analysis and calcimetry of 852 cutting samples as well as four cored sections. Additionally, palynological, magnetic susceptibility and X-ray diffraction analyses were conducted in order to complement the dataset. The lower section of the investigated borehole sequence mainly consists of grey calcareous shale while the middle section is dominated by blue-grey shaly limestone and the upper section is mainly composed of green shale. Palynomorphs found at -5261 m suggest a latest Givetian–Early Frasnian age. A new lithostratigraphical interpretation of the deepest part of the Saint-Ghislain borehole is proposed. The lower calcareous shale from ‑5403 to ‑5100 m is interpreted as the Bovesse Formation (Lower Frasnian) and at its base possibly uppermost Givetian. The overlying limestones from -5100 to -4790 m can be attributed to the Rhisnes Formation (Upper Frasnian), and the green shale between ca. -4393 and -4790 m, to the Bois de la Rocq Member (Famennian). These results open new insights regarding the geological interpretation of the basement underlying the Mons Basin. They also present a promising approach and example regarding interpretations based on cuttings.
Contribuições das funções sociais do professor na construção do ser profissional
Authors: Santos Cláudia Santana , Givigi Rosana Carla Nascimento 
Published: April 2020

Este artigo objetiva compreender como as múltiplas funções sociais do professor moldam seu exercício profissional. Para tanto, utiliza os construtos da teoria sociológica de Norbert Elias, que parte de sua teoria figuracional, refletindo como as cadeias de interdependências apresentam-se nos contextos escolares com grupos funcionalmente dependentes. Trata-se de um trabalho reflexivo, de base bibliográfica, tendo o alicerce os escritos de Nobert Elias, destacando o Processo Civilizador, A solidão dos moribundos, e A sociedade dos indivíduos, de Norbert Elias, além de artigos e trabalhos publicados que voltem-se ao estudo do autor. A proposta foi uma reflexão sobre a formação do professor, que antes de assumir a função de docente tem sua ação configurada por uma ideologia social e pessoal. Portanto, a análise das teias de interdependência e das configurações pode dar visibilidade as problemáticas presentes na função do professor e contribuir para que a teia social se equilibre.
Acute Delirium Induced by Ciprofloxacin in a Patient With Chronic Kidney Disease
Authors: Stroud Sarah G. , Kandemir Utku 
Published: April 2020

Case:
Ciprofloxacin is increasingly used as oral suppressive therapy for musculoskeletal infections. Delirium and acute hypoglycemia are little-known, severe potential adverse drug reactions. We report here on a patient who experienced both complications. The patient is a 56-year-old woman with no psychiatric history who was managed for left lower extremity chronic osteomyelitis after fracture fixation. She developed significant, prolonged delirium and intermittent hypoglycemia within 24 hours of starting ciprofloxacin; both symptoms remitted within 24 hours of drug discontinuation.

Conclusions:
The neuropsychiatric complications of ciprofloxacin are under-discussed, given its frequency of use, and merit greater awareness for the prevention of postoperative delirium.
GWAS and co-expression network combination uncovers multigenes with close linkage effects on oleic acid content accumulation in Brassica napus
Authors: Yao Min , Guan Mei , Zhang Zhenqian , Zhang Qiuping , Cui Yixin , Chen Hao , Liu Wei , Jan Habib U. , Voss-Fels Kai P. , Werner Christian R. , He Xin , Liu Zhongsong , Guan Chunyun , Snowdon Rod J. , Hua Wei , Qian Lunwen 
Published: April 2020

Abstract

Background: Strong artificial and natural selection causes the formation of highly conserved haplotypes that harbor agronomically important genes. GWAS combination with haplotype analysis has evolved as an effective method to dissect the genetic architecture of complex traits in crop species. Results: We use the 60K Brassica Infinium SNP array to perform a genome-wide analysis of haplotype blocks associated with oleic acid (C18:1) in rapeseed. Six haplotype regions were identified as significantly associated with oleic acid (C18:1) that mapped to chromosomes A02, A07, A08, C01, C02, and C03. Additionally, whole-genome sequencing of 50 rapeseed accessions revealed three genes ( BnmtACP2-A02 , BnABCI13-A02 and BnECI1-A02 ) in the A02 chromosome haplotype region and two genes ( BnFAD8-C02 and BnSDP1-C02 ) in the C02 chromosome haplotype region that were closely linked to oleic acid content phenotypic variation. Moreover, the co-expression network analysis uncovered candidate genes from these two different haplotype regions with potential regulatory interrelationships with oleic acid content accumulation. Conclusions: Our results suggest that several candidate genes are closely linked, which provides us with an opportunity to develop functional haplotype markers for the improvement of the oleic acid content in rapeseed.
A large opening and high flow rate piezoelectric pump with straight arm wheeled check valve
Authors: He Lipeng , Wu Xiaoqiang , Wang Zhe , Zhao Da , Wen Jianming , Tian Xiaochao 
Published: April 2020

Abstract
Piezoelectric pumps are applied in cooling systems of microelectronic devices because of their small size. However, cooling efficiency is limited by low flow rate. A Straight arm wheeled check valve made of silica gel was proposed, which can improve flow rate of piezoelectric pump, solve the influence of glue aging on the sealing ability of a wheeled check valve and reduce the size of piezoelectric pump. This paper discusses the influence of valve arm number (N=2, 3 and 4), valve arm width (W=1.0, 1.2 and 1.4mm) and valve thickness (T=0.6, 0.8 and 1.0mm) on flow rate characteristics of piezoelectric pumps. When valve opening rises, the flow rate increases. The simulation results show that valves with 2 valve arms, 0.6mm valve thickness and 1.0mm valve arm width have maximum valve opening. Experimental results show that piezoelectric pumps with different valve parameters have different optimal frequencies. In addition, maximum flow rate is 431.6mL/min at 220V and 70Hz. This paper provides a reference for the application of piezoelectric pump in cooling system.
Serum uric acid: Association with anthropometric, lipid and complete blood count parameters in Iranian healthy population
Authors: Soori Hamid , Rezapour Parinaz , Najafimehr Hadis , Alirezaei Toktam 
Published: April 2020

Abstract
Objectives A relationship between elevated serum uric acid (SUA) and hypertension, metabolic syndrome and cardiovascular disease has been established. In this study, the relationship of SUA levels and anthropometric measures, serum lipid profile and neutrophil to lymphocyte ratio (NLR) and platelet to lymphocyte ratio (PLR) was examined.Methods Anthropometric parameters including body-mass index (BMI), waist circumference (WC), waist to height ratio(WHtR), waist to hip ratio(W/H), waist to pelvic ratio(W/P), neck circumference(NC), body fat mass(BFM) were obtained, and serum lipid profile containing, low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), total cholesterol, triglycerides(TG), also, NLP, PLR, basal metabolic rate (BMR), visceral fat level (VFL) and percent body fat (PBF) were measured among 2921 healthy subjects of young and middle-aged Iranian people. Statistical analysis was performed using SPSS 21 software. To assess the normality of data, the Kolmogorov–Smirnov test was used. Logarithmic transformation was performed for some variables with non-normal distribution. The association between 2 quantitative variables was measured using bivariate correlation (Pearson or Spearman). Pearson correlations and multiple regression analysis were performed to assess the correlation between variables. Simple and multiple regression analyses were performed to predict some variables. P- value <0.05 was considered significant.Results There were 1113 males (mean age, 41.49 ± 8.62 years) and 1808 females (mean age, 42.36 ± 9.07 years) in this study. the male group had a mean SUA level of 4.81± 1.2 mg/dl and the female group had a mean of 4.76 ± 1.1 mg/dl. The results of data analysis showed all studied factors were correlated with SUA level except VFL, BFM, and PLR. The highest correlation was related to skinfold fat thickness, BMR and HDL.Conclusion According to the finding of this study, SUA level measurement might be advisable in healthy population to identify those at increased risk of health problems who might benefit from further evaluation.
University Students’ intention to quit substace abuse in Ethiopian: Application of Theory of Planned Behavior
Authors: gizaw abraham tamirat , Mekuria Misgun Shewangizaw , Abraha Getachew Kiros , Weldemariam Hailemariam Hailesilasie , Gebretsadik Lakew Abebe 
Published: April 2020

Abstract
Background Currently, substance abuse is one of the most burning public health problems in Ethiopia. However, the magnitude of substance abuse and intention to quit is not yet properly studied, Therefore, this study aims to assess University students’ intention to quit substace abuse in Ethiopian:Application of Theory of Planned Behavior (TPB) Methods Institution based cross-sectional study was conducted using self-administered questioners. A stratified sampling technique was used to select the study participants. Descriptive statistics were computed and a multivariate logistic regression model was fitted. A p-value of less than 0.05 was used to declare statistical significance. Result From a total of 392 respondents, 175 (44.6%) were fulfilled the criteria of psychoactive substance abuse (CAGE ≥ 2). Socio-demographic variables such as male respondents (Adjusted Odds Ratio (AOR) =2.34, 95% confidence interval, CI [1.22, 4.51]; P=0.000), Respondents among age category of 25-29 years (Adjusted Odds Ratio(AOR)=21.93, 95% confidence interval CI[16.19; 33.11]; P=0.000) and students with five and above years of study (Adjusted Odds Ratio (AOR)=5.71, 95% confidence interval, CI[2.41-13.52]; P=0.000) had significant association with the psychoactive substance abuse. Theory of Planned Behavior constructs such as positive attitude (Adjusted Odds Ratio[AOR]=1.81,95% confidence interval, CI[1.11,2.96]; P =0.018),supportive Subjective Norm (Adjusted Odds Ratio[AOR]=2.05, 95% confidence interval, CI[1.26,3.47]; P =0.004) and positive Perceived Behavioral Control (Adjusted Odds Ratio [AOR]=6.16, 95% confidence interval, CI[3.75,10.14]; P =0.000) had significant association with quitting psychoactive substance abuse. Conclusion This study illustrated that intention to quit psychoactive substance abuse was predicted by a positive attitude, supportive subjective norm, and positive perceived behavioral control. Therefore, applying health communication strategies focusing on attitude, subjective norm and perceived behavioral control by segmenting students based on sex, age and years of studies are important to bring desired behavioral changes.
USP51, an oncogene for pancreatic cancer (PC) cell growth via Wnt/β-catenin pathway
Authors: Hua Yong-qiang , He Yun , Ding Xiao-bo , Zhang Ke , Sheng Jie , Xu Li-tao , Ning Zhou-yu , Chen Hao , Chen Zhen , Meng Zhi-qiang , Zheng Qing-xing , Liu Lu-Ming 
Published: April 2020

Abstract
Background: Currently, targeting ubiquitin-specific protease (USP) is popular in cancer treatment. Aim: To study the roles of USP51, a member of USPs, in human pancreatic cancer (PC) and the underlying mechanism.Method: Expression of USP51 in tumor pancreatic tissue and PC cell lines were assessed. Overall survival rate of PC patients was measured by Kaplan-Meier method. The association between USP51 and β-catenin in PC cells was determined by Co-Immunoprecipitation (Co-IP) and ubiquitination assay. XAV939 was used to inhibit β-catenin. Protein levels of β-catenin and Cyclin D1 were used to assess the mechanism.Result: Herein, we evidenced high levels of USP51 in tumor tissues of PC patients, which predicted a poor survival. Knockdown of USP51 in MiaPaca2 and SW1990 (two PC cells) inhibited proliferation, arrested MiaPaca2 and SW1990 cells at G0/G1 phases, and impaired tumorigenicity of MiaPaca2 in nude mice, demonstrating the anti-cancer effect of siUSP51 in PC. Moreover, Knockdown of USP51 prevented β-catenin and its downstream Cyclin D1, suggesting the blockade of β-catenin pathway in this process. On the contrary, overexpression of USP51 in ASPC1 (one PC cell) resulted in the opposite effect, facilitating the tumorigenesis of PC and the activation of β-catenin pathway, which were significantly reversed by XAV939. Interestingly, USP51 bound to β-catenin and knockdown of USP51 increased β-catenin ubiquitination.Conclusion: USP51 is an oncogene that is upregulated in PC. USP51 acted as a deubiquitinase that favored β-catenin accumulation, then activated Wnt/β-catenin pathway and exerted carcinogenesis in PC.
Non-destructive Analysis of Volatile Compounds for Geographical Identification of Rice
Authors: Hu Shengying , Ren Hongbo , Yong Song , Gao Siyuan , Meng Li 
Published: April 2020

Abstract
Background In recent years, high-quality rice adulteration has become a serious problem. It is essential to prevent false origin labels and dishonest transactions. However, there is still a lack of rapid identification methods for discriminating rice from different sources. In this study, we developed a method to profile volatile organic compounds (VOCs) using headspace solid phase microextraction (HS-SPME) combined with gas chromatography mass spectrometry (GC-MS). In addition, the identification efficiency of the biomarkers was determined using several multivariate analysis methods. Results Based on the t-test, fold changes and volcano plots, eight typical biomarkers were used based their differential levels. Among them, 2-acetyl-1-pyrroline (2-AP) is the most important source of aroma in rice flavor. Unsupervised analyses, including principal component analysis (PCA) and Cluster analysis, demonstrated the potential for geographic classification of rice between Wuchang and other regions. In addition, partial least squares discriminant analysis (PLS-DA) yielded a goodness of fit of 0.900, a goodness of prediction of 0.853, and a probability of substitution test of 0.012. Random forest (RF) algorithm further strengthened the discriminating ability of volatile compounds. Conclusion In short, the current method can quickly distinguish rice from Wu Chang and other regions, and the research method can facilitate controlling the authenticity and quality of rice.
Cytotoxicity of fractured quartz on human macrophages: role of the membranolytic activity of quartz and phagolysosome destabilization.
Authors: Leinardi Riccardo , Pavan Cristina , Yedavally Harita , Tomatis Maura , Salvati Anna , Turci Francesco 
Published: April 2020

Abstract
Background The pathogenicity of quartz involves lysosomal alteration in alveolar macrophages. This event triggers the inflammatory cascade that may lead to quartz-induced silicosis and eventually lung cancer. Recently, we showed that synthetic quartz induces membrane lysis in red blood cells and cytotoxic responses in murine alveolar macrophages, only when the atomic order of crystal surfaces is upset by fracturing. Cytotoxicity was not observed when quartz exhibited as-grown, unfractured surfaces. These findings raised questions on the potential impact of quartz surfaces on the phagolysosomal membrane upon internalization of the particles by macrophages.Results To gain insights on the surface-induced cytotoxicity of quartz, as-grown and fractured quartz particles in respirable size, differing only in surface properties related to fracturing, were prepared and physico-chemically characterized. Their effects were compared to a well-known toxic commercial quartz dust. Membranolysis was assessed on red blood cells, and quartz uptake, cell viability, and impact on lysosomes were assessed on human PMA-differentiated THP-1 macrophages. All quartz samples were internalized, but only fractured quartz elicited cytotoxicity and phagolysosomal alterations. These effects were blunted when uptake was suppressed. Membranolysis, but not cytotoxicity, was quenched when fractured quartz surface was masked with serum proteins and incubated with cells.Conclusions Upon internalization, the phagolysosome environment rapidly removes serum proteins from quartz surface, restoring quartz membranolytic activity in the phagolysosomes. Fractured quartz induces cytotoxicity in THP-1 human macrophages by promoting phagolysosomal membrane alterations.
Impaired trophoblast Toll-Like Receptor 3 signaling pathway involves in hepatitis B vaccine non- or hypo-response of infants born to HBsAg-positive mothers
Authors: Wu Lina , Zhang Ruijun , Feng Yongliang , Yao Tian , Yi Linzhu , Wan Huili , Li Junli , Feng Shuying , Wang Bo , Wang Suping 
Published: April 2020

Abstract
Background: Babies born to hepatitis B surface antigen (HBsAg) positive mothers bear a high risk of being non- or hypo-responsive to hepatitis B (HB) vaccine with unilluminated mechanisms. Placental immunity is closely related to the development of baby immune system, however, the roles of the placental immunity in the insufficient response of these babies are unclear. This study was aimed to investigate the role of placental trophoblast Toll-Like Receptor 3（TLR3）signaling pathway in HB vaccine non- or hypo-response of these special babies. Methods: A total of 399 pairs of HBsAg-positive mothers and their neonates were recruited to perform a nested case-control study. The maternal and children’s HBV DNA and the HBV serological markers were detected by Fluorescence Quantitative Polymerase Chain Reaction (FQ-PCR) and Electrochemiluminescence Immunoassay (ECLIA). The trophoblast TLR3 signaling pathway proteins and infant cytokines IL-6, IL-12, TNF-α, IFN-α and IFN-γ were tested by immunohistochemistry (IHC) and Enzyme–Linked Immunosorbent Assay (ELISA). Results: The expression of TLR3 and NF-κB, a TLR3 downstream protein, were significantly decreased in the non- or hypo-responders ( Z= -3.00 and -2.46, P <0.01 and =0.01). Furthermore, the trophoblast TLR3 expression negatively correlated with maternal HBV DNA ( r = -0.29, P = 0.003), HBeAg ( r = -0.28, P = 0.01) and HBV DNA+HBeAg ( r = -0.24, P = 0.02). Besides, NF-κB positively correlated with infant IL-6 ( r = 0.24, P = 0.026). By comprehensive analysis of maternal, placental and infant information, a Bayesian network model showed that the trophoblast TLR3 signaling pathway contacted with the non- or hypo-responsiveness. onclusions: Maternal HBV infection affected the trophoblast TLR3 signaling pathway protein expression, and consequently the impaired TLR3 signaling pathway involved in the HB vaccine non- or hypo-responsiveness mainly by influencing infant IL-6.