bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptome and Parasitome Analysis of Beet Cyst Nematode Heterodera schachtii
Authors: Elashry Abdelnaser M. , Habash Samer S. , Vijayapalani Paramasivan , Brocke-Ahmadinejad Nahal , Blümel Roman , Seetharam Arun , Schoof Heiko , Grundler Florian M. W. 
Published: February 2020

AbstractBeet cyst nematodes depend on a set of secretory proteins (effectors) for the induction and maintenance of their syncytial feeding sites in plant roots. In order to understand the relationship between the beet cyst nematode H. schachtii and its host, identification of H. schachtii effectors is crucial and to this end, we sequenced a whole animal pre-infective J2-stage transcriptome in addition to pre- and post-infective J2 gland cell transcriptome using Next Generation Sequencing (NGS) and identified a subset of sequences representing putative effectors. Comparison between the transcriptome of H. schachtii and previously reported related cyst nematodes and root-knot nematodes revealed a subset of esophageal gland related sequences and putative effectors in common across the tested species. Structural and functional annotation of H. schachtii transcriptome led to the identification of nearly 200 putative effectors. Six putative effector expressions were quantified using qPCR and three of them were functionally analyzed using RNAi. Phenotyping of the RNAi nematodes indicated that all tested genes decrease the level of nematodes pathogenicity and/or the average female size, thereby regulating cyst nematode parasitism. These discoveries contribute to further understanding of the cyst nematode parasitism.
Innate T-αβ lymphocytes as new immunological components of anti-tumoral “off-target” effects of the tyrosine kinase inhibitor dasatinib
Authors: Barbarin Alice , Abdallah Myriam , Lefèvre Lucie , Piccirilli Nathalie , Cayssials Emilie , Roy Lydia , Gombert Jean-Marc , Herbelin André 
Published: February 2020

AbstractKinase inhibitors hold great potential as targeted therapy against malignant cells. Among them, the tyrosine kinase inhibitor dasatinib is known for a number of clinically relevant off-target actions, attributed in part to effects on components of the immune system, especially conventional T-cells and natural killer (NK)-cells. Here, we have hypothesized that dasatinib also influences non-conventional T-αβ cell subsets known for their potential anti-tumoral properties, namely iNKT cells and the distinct new innate CD8 T-cell subset. In mice, where the two subsets were originally characterized, an activated state of iNKT cells associated with a shift toward an iNKT cell Th1-phenotype was observed after dasatinib treatment in vivo. Despite decreased frequency of the total memory CD8 T-cell compartment, the proportion of innate-memory CD8 T-cells and their IFNγ expression in response to an innate-like stimulation increased in response to dasatinib. Lastly, in patients administered with dasatinib for the treatment of BCR-ABL-positive leukemias, we provided the proof of concept that the kinase inhibitor also influences the two innate T-cell subsets in humans, as attested by their increased frequency in the peripheral blood. These data highlight the potential immunostimulatory capacity of dasatinib on innate T-αβ cells, thereby opening new opportunities for chemoimmunotherapy.
Preparation of a magnetic polystyrene nanocomposite for dispersive solid-phase extraction of copper ions in environmental samples
Authors: Mehdinia Ali , Salamat Maede , Jabbari Ali 
Published: February 2020

AbstractThe core shell nanostructure of magnetic polystyrene (PS@Fe3O4) was prepared and its physic-chemical properties were studied FT-IR, SEM, TEM, VSM and BET + BJH. The new adsorbent was applied in the dispersive solid phase extraction technique for measuring copper ions in water, Soil and Oyster samples. Analysis is carried out using a flame atomic absorption spectrometry system. Effective parameters on extraction efficiency, such as pH of extraction solution, sorbent dosage, contact time, concentration and volume of desorption eluent and desorption time were optimized using one at a time method. N2 adsorption-desorption experiment resulted in high BET surface area (32.002 m2 g−1) and large pore volume (0.1794 cm3 g−1) for PS@ Fe3O4 nanocomposite. Under the optimum conditions, a calibration curve within the range of 5–40 ng mL−1 with an appropriate coefficient of determination (R2) of 0.9946 was obtained. Preconcentration factor (PF) and limit of detection (LOD) were found to be 55 and 1.6 ng mL−1, respectively. The repeatability and reproducibility for three replicate measurements at the concentration of 25 ng mL−1 were 2.5%–1.4%, respectively. The Freundlich adsorption isotherm and pseudo-second-order kinetic model were consistent to experimental data in adsorption mechanism study. The maximum adsorption capacity was 19.56 mg g−1 for Cu (II). Finally, the efficiency of the method was investigated for analysis of the copper in environmental samples and good relative recoveries (RR%) were obtained within the range of 99.2% to 101.2%.
Wideband Miniaturized Design of Complementary Spoof Surface Plasmon Polaritons Waveguide Based on Interdigital Structures
Authors: Pan Bai Cao , Luo Guo Qing , Liao Zhen , Cai Jia Lin , Cai Ben Geng 
Published: February 2020

AbstractIn this paper, we present to achieve a broadband miniaturized transmission waveguide based on complementary spoof surface plasmon polaritons (CSSPPs). For this purpose, a novel SSPP design that consists of a corrugated slot line and a group of additional interdigital structures (ISs) is proposed, which brings in an extra solution to control the cut-off property of CSSPPs. The transmission cut-off frequency of the proposed design decreases with the increasing of the number of the ISs. Since the width of CSSPP waveguide is directly related to the operating frequency, the degree of miniaturization can be modulated freely by carefully choosing the number of the ISs. A prototype of device with four-ISs introduced is designed and fabricated. And the cut-off frequency of the design decreases from 10 GHz to 5.3 GHz, when the ISs are added. Experimental results agree well with the numerical simulations. The proposed design illustrates great potentials in modern plasmonic integrated circuits.
Large-scale assessment of human navigation ability across the lifespan
Authors: van der Ham Ineke J. M. , Claessen Michiel H. G. , Evers Andrea W. M. , van der Kuil Milan N. A. 
Published: February 2020

AbstractNavigation ability is particularly sensitive to aging. Evidence of aging patterns is largely restricted to comparing young adults and elderly and limited in the variety of navigation tasks used. Therefore, we designed a novel task battery to assess navigation ability in a very large, representative sample (N = 11,887, 8–100 years). The main aim was to measure navigation ability across the lifespan in a brief, yet comprehensive manner. Tasks included landmark knowledge, egocentric and allocentric location knowledge, and path knowledge for a route and survey perspective. Additionally, factors that potentially contribute to navigation ability were considered; gender, spatial experience and spatial anxiety. Increase in performance with age in children was found for allocentric location knowledge and for route-based path knowledge. Age related decline was found for all five tasks, each with clearly discernible aging patterns, substantiated the claim that each task distinctively contributes to the assessment of navigation ability. This study provides an in depth examination of navigation ability across dissociable functional domains and describes cognitive changes across the lifespan. The outcome supports the use of this task battery for brief assessment of navigation for experimental and clinical purposes.
Irritable bowel, chronic widespread pain, chronic fatigue and related syndromes are prevalent and highly overlapping in the general population: DanFunD
Authors: Petersen Marie Weinreich , Schröder Andreas , Jørgensen Torben , Ørnbøl Eva , Meinertz Dantoft Thomas , Eliasen Marie , Benros Michael Eriksen , Fink Per 
Published: February 2020

AbstractPrevalence of functional somatic syndromes (FSS) in the general population varies with observed overlap between syndromes. However, studies including a range of FSS are sparse. We investigated prevalence and characteristics of various FSS and the unifying diagnostic construct bodily distress syndrome (BDS), and identified mutual overlap of the FSS and their overlap with BDS. We included a stratified subsample of 1590 adults from a randomly selected Danish general population sample (n = 7493). Telephonic diagnostic interviews performed by three trained physicians were used to identify individuals with FSS and BDS. Prevalence of overall FSS was 9.3%; 3.8% for irritable bowel, 2.2% for chronic widespread pain, 6.1% for chronic fatigue, 1.5% for whiplash associated disorders, and 0.9% for multiple chemical sensitivity. Prevalence of BDS was 10.7% where 2.0% had the multi-organ type. FSS were highly overlapping with low likelihood of having a “pure” type. Diagnostic agreement of FSS and BDS was 92.0%. Multi-syndromatic FSS and multi-organ BDS were associated with female sex, poor health, physical limitations, and comorbidity. FSS are highly prevalent and overlapping, and multi-syndromatic cases are most affected. BDS captured the majority of FSS and may improve clinical management, making the distinction between multi- and mono-syndromatic patients easier.
Homalomena pineodora essential oil nanoparticle inhibits diabetic wound pathogens
Authors: Rozman Nur Amiera Syuhada , Tong Woei Yenn , Leong Chean Ring , Anuar Mohd Razealy , Karim Sabrina , Ong Siew Kooi , Yusof Fahmi Asyadi Md , Tan Wen-Nee , Sulaiman Baharuddin , Ooi Mei Lee , Lee Kok Chang 
Published: February 2020

AbstractEssential oil of Homalomena pineodora inhibits diabetic pathogens; however, the activity was not sustainable when applied as wound dressing. This study aims to synthesise the essential oil nanoparticle using chitosan. The nanoparticles were synthesised with ion gelation method, confirmed by spectroscopic analysis. The spherical nanoparticles display a size of 70 nm, with strong surface charge of +24.10 mV. The nanoparticles showed an initial burst release followed by a slow release pattern for 72 h, following the first order of kinetic. The release behaviour was ideal for wound dressing. The antimicrobial activity was broad spectrum. The formation of nanoparticle enhanced the antimicrobial efficacy of the essential oil. The nanoparticle also showed a concentration-dependent killing behaviour on time–kill assay. In the 3D collagen wound models, the nanoparticles reduced the microbial growth by 60–80%. In conclusion, H. pineodora nanoparticles showed pharmaceutical potential in inhibiting microbial growth on diabetic ulcers.
Resistive switching studies in VO2 thin films
Authors: Rana Abhimanyu , Li Chuan , Koster Gertjan , Hilgenkamp Hans 
Published: February 2020

AbstractThe hysteretic insulator-to-metal transition of VO2 is studied in detail for pulsed laser deposition grown thin films on TiO2 substrates, under variation of temperature and applied bias currents. This system is of interest for novel electronics based on memristive concepts, in particular as the resistive transition in these films occurs close to room temperature. Multiple, stable resistance states can be set controllably in the temperature range of the hysteretic phase transition by tailored temperature sweeps or by Joule heating induced by current pulses.
Droplet-based digital antibiotic susceptibility screen reveals single-cell clonal heteroresistance in an isogenic bacterial population
Authors: Scheler Ott , Makuch Karol , Debski Pawel R. , Horka Michal , Ruszczak Artur , Pacocha Natalia , Sozański Krzysztof , Smolander Olli-Pekka , Postek Witold , Garstecki Piotr 
Published: February 2020

AbstractSince antibiotic resistance is a major threat to global health, recent observations that the traditional test of minimum inhibitory concentration (MIC) is not informative enough to guide effective antibiotic treatment are alarming. Bacterial heteroresistance, in which seemingly susceptible isogenic bacterial populations contain resistant sub-populations, underlies much of this challenge. To close this gap, here we developed a droplet-based digital MIC screen that constitutes a practical analytical platform for quantifying the single-cell distribution of phenotypic responses to antibiotics, as well as for measuring inoculum effect with high accuracy. We found that antibiotic efficacy is determined by the amount of antibiotic used per bacterial colony forming unit (CFU), not by the absolute antibiotic concentration, as shown by the treatment of beta-lactamase-carrying Escherichia coli with cefotaxime. We also noted that cells exhibited a pronounced clustering phenotype when exposed to near-inhibitory amounts of cefotaxime. Overall, our method facilitates research into the interplay between heteroresistance and antibiotic efficacy, as well as research into the origin and stimulation of heterogeneity by exposure to antibiotics. Due to the absolute bacteria quantification in this digital assay, our method provides a platform for developing reference MIC assays that are robust against inoculum-density variations.
Activation of the IL-1β/KLF2/HSPH1 pathway promotes STAT3 phosphorylation in alveolar macrophages during LPS-induced acute lung injury
Authors: Liang Yafeng , Luo Jiaqi , Yang Nengli , Wang Shufen , Ye Mingwei , Pan Guoquan 
Published: February 2020

Abstract
Acute lung injury (ALI) is a lethal disease with diffuse lung inflammation, in which JAK/STAT3 signaling has been well recognized for its role in initiating and amplifying inflammatory processes. However, the mechanism for the enhancement and maintenance of signal transducer and activator of transcription 3 (STAT3) activation has not yet been clearly demonstrated in ALI. In the present work, we established a lipopolysaccharide (LPS)-induced ALI rat model through intratracheal instillation and isolated the alveolar macrophages (AMs) from the rats in the model. We demonstrated that the expression of Kruppel-like factor 2 (KLF2) significantly decreased in the AMs from LPS-induced ALI rats (LPS-AMs) as compared with the AMs from control rats (NC-AMs). Overexpressing KLF2 in LPS-AMs inhibited the phosphorylation of STAT3 and reduced the levels of STAT3 target genes, including matrix metalloproteinase (MMP)-2/9 (MMP-2/9). Further investigation indicated that KLF2 trans-inhibited heat shock protein H1 (HSPH1), which interacted with STAT3 and enhanced its phosphorylation. As a crucial inflammatory mediator in ALI, interleukin-1β (IL-1β) induced the down-regulation of KLF2 in LPS-AMs, as interrupting IL-1β signaling in LPS-AMs by antibody neutralization or IL1R1 knockdown rescued the expression of KLF2. Consistently, stimulating NC-AMs with IL-1β decreased KLF2 and increased HSPH1, while overexpression of KLF2 suppressed IL-1β-induced HSPH1. Additionally, in vivo studies showed that treatment with an IL-1β antibody or HSPH1 inhibitor alleviated lung injury in ALI rats, as well as decreased the levels of p-STAT3 and MMP-2/9. In conclusion, activation of the IL-1β/KLF2/HSPH1 pathway facilitated STAT3 phosphorylation in AMs, which exacerbated pulmonary inflammation in ALI.
Quantifying the latitudinal representivity of in situ solar wind observations
Authors: Owens Mathew J. , Lang Matthew , Riley Pete , Lockwood Mike , Lawless Amos S. 
Published: February 2020

Advanced space-weather forecasting relies on the ability to accurately predict near-Earth solar wind conditions. For this purpose, physics-based, global numerical models of the solar wind are initialized with photospheric magnetic field and coronagraph observations, but no further observation constraints are imposed between the upper corona and Earth orbit. Data assimilation (DA) of the available in situ solar wind observations into the models could potentially provide additional constraints, improving solar wind reconstructions, and forecasts. However, in order to effectively combine the model and observations, it is necessary to quantify the error introduced by assuming point measurements are representative of the model state. In particular, the range of heliographic latitudes over which in situ solar wind speed measurements are representative is of primary importance, but particularly difficult to assess from observations alone. In this study we use 40+ years of observation-driven solar wind model results to assess two related properties: the latitudinal representivity error introduced by assuming the solar wind speed measured at a given latitude is the same as that at the heliographic equator, and the range of latitudes over which a solar wind measurement should influence the model state, referred to as the observational localisation. These values are quantified for future use in solar wind DA schemes as a function of solar cycle phase, measurement latitude, and error tolerance. In general, we find that in situ solar wind speed measurements near the ecliptic plane at solar minimum are extremely localised, being similar over only 1° or 2° of latitude. In the uniform polar fast wind above approximately 40° latitude at solar minimum, the latitudinal representivity error drops. At solar maximum, the increased variability of the solar wind speed at high latitudes means that the latitudinal representivity error increases at the poles, though becomes greater in the ecliptic, as long as moderate speed errors can be tolerated. The heliospheric magnetic field and solar wind density and temperature show very similar behaviour.
Operationen und akute Erkrankungen bei Typ-1-Diabetes
Authors: Thiele Alena Gerlinde , Heckenmüller Maren , Bartelt Heike , Klamt Sabine , Kapellen Thomas Michael 
Published: February 2020

ZUSAMMENFASSUNGEine kontinuierliche intravenöse Insulininfusionstherapie minimiert Blutzucker (BZ)-Schwankungen und damit das Risiko für Stoffwechselentgleisungen bei Kindern und Jugendlichen mit Diabetes mellitus Typ 1 (T1D), während einer Operation oder einer akuten Zweiterkrankung. Die Datenlage hinsichtlich sicherer BZ-Zielbereiche, adäquater Flüssigkeit- und Insulindosierungen während einer Insulininfusion bei pädiatrischen Patienten ist jedoch spärlich. Das hauseigene Schema zur Insulinfusionstherapie wurde nun hinsichtlich seiner Effektivität und Sicherheit in 124 Fällen bei 62 Patienten (n = 78 chirurgische Eingriffe, n = 46 akute Zweiterkrankung, mittleres Alter 9,6 ± 5,4 Jahre) evaluiert. In > 60 % der Infusionszeit lagen die BZ-Werte im Zielbereich, in nur 6 % der Zeit kam es zu kritischen Hypo- und Hyperglykämien. Damit erwies sich das Protokoll als effektiv und sicher. Allerdings erlebten Kinder < 12 Jahre signifikant mehr kritische Hypoglykämien im Vergleich zu Jugendlichen (Hypoglykämie/Fall 2,4 ± 2,7 vs. 0,9 ± 2,0; p < 0,001). Daher erscheint das Schema für Jugendliche geeigneter zu sein. Wir haben folglich das Protokoll für jüngeren Patienten durch Reduktion der Insulindosierung angepasst.
Constructing the digital self in the Global South
Authors: Campbell Baird , Haynes Nell 
Published: February 2020

Abstract
The papers in this special section examine how people in various contexts of the Global South “construct the self” in
online spaces. With examples from Chile, Senegal, and Trinidad, the papers show the wide range of discursive practices, encompassing the
textual and the aesthetic, which individuals use to enact gendered and sexual selves online. By privileging gender and sexuality as central
components of selfhood, we draw from the longstanding attention paid to gender and sexuality in linguistic studies of identification (see
Bucholtz & Hall 2004). In placing this concept within digital worlds, we pay attention to
the ways in which daily life is now lived and experienced online. Authors in this issue think critically about practices of self-formation
and the performance of gender and sexuality that differ from those that have normalized in the Global North, considering both revolutionary
possibility, and re-entrenchment of constraint.
Supporting Erasmus students through integrating reflective practicesin the curriculum
Authors: Holfter Gisela , Rieder Maria 
Published: February 2020

Abstract
There is considerable evidence that suitable preparation for the Erasmus experience can make a significant difference to students. Often, however, institutions have to battle to provide in-depth pre-departure training, and re-entry events or training are too seldom available. Following Erasmus there is rarely space in the curriculum of the home university for reflection, assessment and the integration of the experiences and multi-faceted learning that took place abroad. This article argues that the challenge is therefore to integrate meaningful reflections and assessment into more traditional modules, such as literature-based ones. Accordingly, this article presents some options of how to integrate re-entry reflections in the form of a module on travel literature as well as a voluntary re-entry module that can be offered to returning students irrespective of their course of study. Components of the Erasmus+ CONNECT training programme serve as an example of training material.
The role of social networks and intense friendships in study abroad students’ L2 use and speaking
development
Authors: Baten Kristof 
Published: February 2020

Abstract
This article examines the connections among self-reported social network development, L2 use, and self-perceived
speaking proficiency development in a group of Belgian ERASMUS students (n = 59) who studied abroad in different
European countries (Austria, France, Germany, Ireland, Italy, Spain, Sweden, and the UK). The results suggest a number of
differences between the participants in this study and the US cohorts who have been traditionally the focus of previous SA
research. For example, the Belgian students report high levels of proficiency in the target language before going abroad and high
levels of target language use while abroad. Furthermore, a number of social network variables point to differences between the
ERASMUS students in the present study and the US students featuring in previous research. Nevertheless, the results also reveal
some similarities, especially with regard to the social network variables ‘size’ and ‘intensity of friendship’ which were
predictors of language gains for the group of students under analysis in this study and, consequently, corroborated findings of
previous studies conducted with US cohorts.
Identification of SFL1 as a positive regulator for flor formation in Zygosaccharomyces rouxii
Authors: Mogi Ryosuke , Watanabe Jun 
Published: February 2020

ABSTRACT
Some wild Zygosaccharomyces rouxii impair the quality of soy sauce through the generation of unpleasant odors induced by the formation of flor. Flor formation in Z. rouxii depends on the expression of the FLO11D gene, which is a homolog of the FLO11 gene that encodes a cell surface protein in Saccharomyces cerevisiae. FLO11 expression in S. cerevisiae is regulated by multiple pathways. To investigate the regulation of FLO11D expression in Z. rouxii, we created 13 gene knockout mutants (STE12, TEC1, HOG1, MSS11, FLO8, MSN1, MSN2/4, SKO1, TUP1, CYC8, YAK1, MIG1, and SFL1) related to those pathways and examined whether these mutants form flor. Unexpectedly, SFL1 knockout mutant could only form a very weak flor due to decreased FLO11D expression, suggesting that SFL1 acts as a potential activator of flor formation through FLO11D expression. This result is in contrast to S. cerevisiae SFL1, which acts as a repressor of FLO11 expression.