bluuebunny/crossref_metadata_2025_split huggingface dataset:
NOS1AP polymorphisms reduce NOS1 activity and interact with prolonged repolarization in arrhythmogenesis
Authors: Ronchi Carlotta , Bernardi Joyce , Mura Manuela , Stefanello Manuela , Badone Beatrice , Rocchetti Marcella , Crotti Lia , Brink Paul , Schwartz Peter J , Gnecchi Massimiliano , Zaza Antonio 
Published: February 2020

Abstract

Aims
NOS1AP single-nucleotide polymorphisms (SNPs) correlate with QT prolongation and cardiac sudden death in patients affected by long QT syndrome type 1 (LQT1). NOS1AP targets NOS1 to intracellular effectors. We hypothesize that NOS1AP SNPs cause NOS1 dysfunction and this may converge with prolonged action-potential duration (APD) to facilitate arrhythmias. Here we test (i) the effects of NOS1 inhibition and their interaction with prolonged APD in a guinea pig cardiomyocyte (GP-CMs) LQT1 model; (ii) whether pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) from LQT1 patients differing for NOS1AP variants and mutation penetrance display a phenotype compatible with NOS1 deficiency.

Methods and results
In GP-CMs, NOS1 was inhibited by S-Methyl-L-thiocitrulline acetate (SMTC) or Vinyl-L-NIO hydrochloride (L-VNIO); LQT1 was mimicked by IKs blockade (JNJ303) and β-adrenergic stimulation (isoproterenol). hiPSC-CMs were obtained from symptomatic (S) and asymptomatic (AS) KCNQ1-A341V carriers, harbouring the minor and major alleles of NOS1AP SNPs (rs16847548 and rs4657139), respectively. In GP-CMs, NOS1 inhibition prolonged APD, enhanced ICaL and INaL, slowed Ca2+ decay, and induced delayed afterdepolarizations. Under action-potential clamp, switching to shorter APD suppressed ‘transient inward current’ events induced by NOS1 inhibition and reduced cytosolic Ca2+. In S (vs. AS) hiPSC-CMs, APD was longer and ICaL larger; NOS1AP and NOS1 expression and co-localization were decreased.

Conclusion
The minor NOS1AP alleles are associated with NOS1 loss of function. The latter likely contributes to APD prolongation in LQT1 and converges with it to perturb Ca2+ handling. This establishes a mechanistic link between NOS1AP SNPs and aggravation of the arrhythmia phenotype in prolonged repolarization syndromes.
Atrial septum stenting in a foetus with hypoplastic left heart syndrome and restrictive foramen ovale: an alternative to emergency atrioseptectomy in the newborn—a case report
Authors: Tulzer Andreas , Arzt Wolfgang , Prandstetter Christoph , Tulzer Gerald 
Published: February 2020

Abstract

Background
In patients with hypoplastic left heart syndrome (HLHS) premature closure or restriction of the interatrial communication causes severe cyanosis directly after birth with rapid deterioration in clinical state. An ex-utero intrapartum treatment (EXIT) procedure, extracorporal membrane oxygenation (ECMO), and emergency interventional cardiac catheterization or cardiac surgery has to be anticipated and prepared. We report the first case performing foetal atrial septum stenting in such a patient directly before birth to enable uncomplicated interatrial shunting postnatally.

Case summary
A 31-year-old pregnant woman was referred to our centre for further evaluation of the foetus due to HLHS. In the follow-up study before birth severe restriction of the foramen ovale with increased retrograde flow in the pulmonary veins [0.33 ratio antegrade/retrograde time velocity integral (TVI)] was detected. After careful consideration foetal atrial septum stenting was performed at 38 + 3 weeks of gestation. At 39 + 2 weeks of gestation the baby was born by caesarean section followed by an uncomplicated postnatal adaption. On the 7th day of life a Norwood procedure was performed and the baby was discharged on the 63rd postoperative day.

Discussion
Evaluation of the interatrial communication in foetuses with HLHS should be done carefully just before birth. In the case of severe restriction or closure of the foramen ovale atrial septum stenting just before birth can be considered as an alternative treatment to an EXIT procedure, ECMO, or emergency atrioseptectomy on bypass. However, a very experienced team of paediatric cardiologists and perinatologists with expertise in foetal cardiac interventions is needed to perform this technical difficult procedure.
Non-bacterial thrombotic endocarditis in the context of pulmonary adenocarcinoma: a case report
Authors: Kaufmann Christoph C , Wessely Emil , Huber Kurt 
Published: February 2020

Abstract

Background
Non-bacterial thrombotic endocarditis (NBTE) is a rare condition, usually observed in association with malignancy, lupus erythematosus, or antiphospholipid syndrome. Diagnosis of NBTE remains a challenge as patients are often asymptomatic up to their first thromboembolic event. While there is no randomized data available for the guidance of treatment in NBTE, effective anticoagulation remains the main focus in the management of affected patients.

Case summary
A 44-year-old female patient without a significant medical history presented to the emergency department with a new numbness of her right hand. Magnetic resonance imaging scans facilitated the diagnosis of supratentorial stroke. Within the next 3 months, the patient had multiple thromboembolic events, including multiple strokes, pulmonary embolism, and renal/splenic infarction. Echocardiographic examination revealed large, transient vegetations of the aortic valve with concomitant aortic regurgitation. In addition, an incidental, pulmonary non-small-cell adenocarcinoma was found during the diagnostic work-up. Infective endocarditis was excluded by several negative blood cultures and missing signs of infection. Hence, the diagnosis of NBTE secondary to malignancy was made.

Discussion
We present a rare case of NBTE in the context of pulmonary adenocarcinoma. The adequate treatment of malignancy and effective anticoagulation are the main treatment options.
How trophic cascades and photic zone nutrient content interact to generate basin-scale differences in the microbial food web
Authors: Thingstad T Frede 
Published: February 2020

Abstract
In linear food chains, resource and predator control produce positive and negative correlations, respectively, between biomass at adjacent trophic levels. These simple relationships become more complex in food webs that contain alternative food chains of unequal lengths. We have used a “minimum” model for the microbial part of the pelagic food web that has three such food chains connecting free mineral nutrients to copepods: via diatoms, autotrophic flagellates, and heterotrophic bacteria. Trophic cascades from copepods strongly modulates the balance between the three pathways and, therefore, the functionality of the microbial food web in services such as food production for higher trophic levels, DOM degradation, and ocean carbon sequestration. The result is a theoretical framework able to explain, not only apparent conflicts in Arctic mesocosm experiments, but also biogeochemical features of the Mediterranean. Here, the fundamental difference between Arctic and Mediterranean microbial food webs is the way they are predator driven by seasonal migration of large copepods in the Arctic, but resource driven due to the anti-estuarine circulation in the Mediterranean. In this framework, global change effects on microbial ecosystem functions are more like to come indirectly through changes in these drivers than through direct temperature effects on the microbes.
Equivalence of Assurance® Gold Enzyme Immunoassay for Visual or Instrumental Detection of Motile and Nonmotile Salmonella in All Foods to AOAC Culture Method: Collaborative Study
Authors: Feldsine Philip T , Mui Linda A , Forgey Robin L , Kerr David E ,   Collaborators:, Al-Hasani S , Arling V , Beatty S , Bohannon J , Brannan J , Brown N , Bryant J , Burford M , Chavez C , Chinault K , Cooan N , Copeland F , Dixon L , Fitzgerald S , Franke W , Frissora R , Gailbreath K , Godon S , Good M , Ha T , Hagen H , Hanson S , Johnson K , Koch S , Leung S , Lienau A , Lin J , Lin S , Marolla B , Maycock L , McDonagh S , Miller L , Otten N , Post R , Resutek J , Rice B , Richter D , Ritger C , Schwantes D , Simon J , Smith J , Smith S , Stokes R , Thibideau J , Tuncan E , Uber D , Van Landingham V , Vrana D , West D 
Published: February 2020

Abstract
Six foods representative of a wide variety of processed, dried powder processed, and raw food types were analyzed by the Assurance® Gold Salmonella Enzyme Immunoassay (EIA) and AOAC INTERNATIONAL culture method. Paired samples of each food type were simultaneously analyzed; one sample by the Assurance method and one by the AOAC culture method. The results for Assurance method were read visually and instrumentally with a microplate reader. A total of 24 laboratories representing federal government agencies and private industry, in the United States and Canada, participated in this collaborative study. Food types were inoculated with species of Salmonella with the exception of raw ground chicken, which was naturally contaminated. No statistical differences (p &lt; 0.05) were observed between Assurance Gold Salmonella EIA with either visual or instrumental interpretation and the AOAC culture method for any inoculation level of any food type or naturally contaminated food. The Assurance visual and instrumental options of reading sample reactions produced the same results for 1277 of the 1296 sample and controls analyzed.
VIDAS® Enzyme-Linked Immunofluorescent Assay for Detection of Listeria in Foods: Collaborative Study
Authors: Gangar Vidhya , Curiale Michael S , D’Onorio Armando , Schultz Ann , Johnson Ronald L , Atrache Vincent ,   Collaborators:, Agin J , Allen M , Armstrong T , Chaney T , Chang P , Chavey C , Clark T , Clover J , Cook P , Copeland F , Courtney T , Davis B , D’Onorio A , Downs D , Fender M , Foster T , Fox W , Hagen H , Hall C , High E , Kalik M , Kallstrom C , Keith M , Kruegel W , Lee J , Lewus C , Light D , Lindgren S , Mills J , Minor J , Murphy M , Muzzy T , Raghubeer E , Robbins R , Salinitro A , Saunders L , Sayer T , Schultz A , Sumpter R , Traux T , Vought K , Witt J , Yonker D 
Published: February 2020

Abstract
The VIDAS LIS method and the traditional culture methods for detection of Listeria species in food were evaluated in a multilaboratory comparative study. The 6 foods tested were either naturally contaminated or inoculated with 3 different concentrations of Listeria. Results for each food and each contamination level with the VIDAS LIS method were as good as or better than those obtained with the traditional culture method. Of 1558 samples tested, 935 were positive: 839 by the VIDAS method and 809 by standard culture methods. Overall false negative rates were 10.3 and 13.5% for the VIDAS LIS and culture methods, respectively. The false positive rate for the VIDAS LIS assay was 1.4% based on 9 VIDAS LIS positive assays that did not confirm positive by isolation of Listeria. The agreement between the VIDAS LIS and culture methods for all samples tested was 86%.
Simultaneous Determination of Chlorothalonil and Hexachlorobenzene in Technical and Formulated Materials by Capillary Gas Chromatography: Collaborative Study
Authors: Vargyas Linda D , Walls Gregory E , Bramstedt William R , Eilrich Gary L ,   Collaborators:, Bennett O , Burns A , Bushway R , Byington J , Denomme M , Fox D , Helfant L , Kennedy D , Ripley B , Schetter J , Schultz W 
Published: February 2020

Abstract
A collaborative study was conducted for the capillary gas chromatographic (GC) method for the simultaneous determination of the fungicide chlorothalonil (CTL) and the accompanying impurity, hexachlorobenzene (HCB), in technical and formulated materials. The method calls for the dissolution of technical and dry formulations of CTL and HCB from the aqueous flowable formulation. The 10 participating laboratories were asked to analyze the samples by adhering to the method as closely as their instrumentation and data systems allowed, and to note any deviations from the method. Collaborators were asked to prepare the standards and samples, set up the capillary GC systems, analyze the samples, and calculate the results. CTL produced reproducibility relative standard deviations (RSDR) of 0.4–2.5 (active ingredient concentrations ranged from approximately 52 to 98% by weight). HCB produced RSDR values of 5.2–22% (HCB concentrations were 0.02–0.04% by weight). The method was adopted First Action by AOAC INTERNATIONAL.
Virulence of Commercialized Fungal Entomopathogens Against Asian Longhorned Beetle (Coleoptera: Cerambycidae)
Authors: Clifton Eric H , Jaronski Stefan T , Hajek Ann E 
Published: February 2020

AbstractNine strains of five species of entomopathogenic hypocrealean fungi were tested against adults of the Asian longhorned beetle, Anoplophora glabripennis (Motschulsky). These strains have been developed as commercial biopesticide products in the United States, Brazil, South Korea, or the European Union (EU). Metarhizium anisopliae (Metschnikoff) (Hypocreales: Clavicipitaceae) ESALQ E-9 and Metarhizium brunneum (Petch) F52 (formerly M. anisopliae F52) (Hypocreales: Clavicipitaceae) killed 100% of treated beetles with the shortest survival times. Virulence differed among the five strains of Beauveria bassiana (Balsamo) (Hypocreales: Cordycipitaceae) tested, ranging from 0 to 77.3% mortality within 28 d. Two Isaria fumosorosea (Wize, 1904) (Hypocreales: Cordycipitaceae) (formerly Paecilomyces fumosoroseus) strains and the Lecanicillium muscarium (Petch) Zare & Gams (Hypocreales: Cordycipitaceae) strain used in Mycotal were not pathogenic to A. glabripennis adults. Within the entomopathogenic fungi tested, the Metarhizium strains may be the most appropriate for further evaluation.
Fast approximate methods for modified gravity cosmological simulations
Authors: Vraštil Michal , Habib Salman 
Published: February 2020

ABSTRACTThe accelerated expansion of the Universe poses a major theoretical puzzle. Although the assumption of a non-zero cosmological constant provides a minimal extension of general relativity that is consistent with observational data, many theories of modified gravity have been suggested as possible alternatives. Predictions of structure formation for these models in the fully non-linear regime are very expensive and it is difficult, if not impossible, to explore such a huge space of models and parameters using high-resolution N-body simulations. Even in the mildly non-linear regime, perturbative methods can become extremely complex. We explore whether simplified dynamical approximations, applicable for a certain set of cosmological probes, can be used to investigate models of modified gravity with acceptable accuracy in the latter instance. For the case of chameleon gravity, we find that these methods can indeed be used to explore the region around the baryon acoustic oscillation scale, $k\sim 0.1~h\, \text{Mpc}^{-1}$ but not much further.
The GALAH survey: chemodynamics of the solar neighbourhood
Authors: Hayden Michael R , Bland-Hawthorn Joss , Sharma Sanjib , Freeman Ken , Kos Janez , Buder Sven , Anguiano Borja , Asplund Martin , Chen Boquan , De Silva Gayandhi M , Khanna Shourya , Lin Jane , Horner Jonathan , Martell Sarah , Ting Yuan-Sen , Wyse Rosemary , Zucker Daniel , Zwitter Tomaz 
Published: February 2020

ABSTRACT
We present the chemodynamic structure of the solar neighbourhood using 55 652 stars within a 500 pc volume around the Sun observed by GALAH and with astrometric parameters from Gaia DR2. We measure the velocity dispersion for all three components (vertical, radial, and tangential) and find that it varies smoothly with [Fe/H] and [α/Fe] for each component. The vertical component is especially clean, with $\sigma _{v_z}$ increasing from a low of 10 km s−1 at solar [α/Fe] and [Fe/H] to a high of more than 50 km s−1 for more metal-poor and [α/Fe] enhanced populations. We find no evidence of a large decrease in the velocity dispersion of the highest [α/Fe] populations as claimed in surveys prior to Gaia DR2. The eccentricity distribution for local stars varies most strongly as a function of [α/Fe], where stars with [α/Fe] &lt; 0.1 dex having generally circular orbits (e &lt; 0.15), while the median eccentricity increases rapidly for more [α/Fe] enhanced stellar populations up to e ∼ 0.35. These [α/Fe] enhanced populations have guiding radii consistent with origins in the inner Galaxy. Of the stars with metallicities much higher than the local interstellar medium ([Fe/H] &gt; 0.1 dex), we find that the majority have e &lt; 0.2 and are likely observed in the solar neighbourhood through churning/migration rather than blurring effects, as the epicyclic motion for these stars is not large enough to reach the radii at which they were likely born based on their metallicity.
The loss of the intracluster medium in globular clusters
Authors: Chantereau W , Biernacki P , Martig M , Bastian N , Salaris M , Teyssier R 
Published: February 2020

ABSTRACT
Stars in globular clusters (GCs) lose a non-negligible amount of mass during their post-main-sequence evolution. This material is then expected to build up a substantial intracluster medium (ICM) within the GC. However, the observed gas content in GCs is a couple of orders of magnitude below these expectations. Here, we follow the evolution of this stellar wind material through hydrodynamical simulations to attempt to reconcile theoretical predictions with observations. We test different mechanisms proposed in the literature to clear out the gas such as ram-pressure stripping by the motion of the GC in the Galactic halo medium and ionization by UV sources. We use the code ramses to run 3D hydrodynamical simulations to study for the first time, the ICM evolution within discretized multimass GC models including stellar winds and full radiative transfer. We find that the inclusion of both ram pressure and ionization is mandatory to explain why only a very low amount of ionized gas is observed in the core of GCs. The same mechanisms operating in ancient GCs that clear the gas could also be efficient at younger ages, meaning that young GCs would not be able to retain gas and form multiple generations of stars as assumed in many models to explain ‘multiple populations’. However, this rapid clearing of gas is consistent with observations of young massive clusters.
High-energy cosmic ray production in X-ray binary jets
Authors: Cooper A J , Gaggero D , Markoff S , Zhang S 
Published: February 2020

ABSTRACT
As smaller analogues of active galactic nuclei, X-ray binaries (XRBs) are also capable of launching jets that accelerate particles to high energies. In this work, we re-examine XRB jets as potential sources of high-energy cosmic rays (CRs) and explore whether they could provide a significant second Galactic component to the CR spectrum. In the most intriguing scenario, XRB-CRs could dominate the observed spectrum above the so-called knee feature at ∼3 × 1015 eV, offering an explanation for several key issues in this transition zone from Galactic to extragalactic CRs. We discuss how such a scenario could be probed in the near future via multimessenger observations of XRB jets, as well as diffuse Galactic neutrino flux measurements.
Extreme ultraviolet quasar colours from GALEX observations of the SDSS DR14Q catalogue
Authors: Vanden Berk Daniel E , Wesolowski Sarah C , Yeckley Mary J , Marcinik Joseph M , Quashnock Jean M , Machia Lawrence M , Wu Jian 
Published: February 2020

ABSTRACT
The rest-frame far to extreme ultraviolet (UV) colour–redshift relationship has been constructed from data on over $480\, 000$ quasars carefully cross-matched between SDSS Data Release 14 and the final GALEX photometric catalogue. UV matching and detection probabilities are given for all the quasars, including dependencies on separation, optical brightness, and redshift. Detection limits are also provided for all objects. The UV colour distributions are skewed redward at virtually all redshifts, especially when detection limits are accounted for. The median GALEX far-UV minus near-UV (FUV − NUV) colour–redshift relation is reliably determined up to z ≈ 2.8, corresponding to rest-frame wavelengths as short as 400 Å. Extreme UV (EUV) colours are substantially redder than found previously, when detection limits are properly accounted for. Quasar template spectra were forward modelled through the GALEX bandpasses, accounting for intergalactic opacity, intrinsic reddening, and continuum slope variations. Intergalactic absorption by itself cannot account for the very red EUV colours. The colour–redshift relation is consistent with no intrinsic reddening, at least for SMC-like extinction. The best model fit has a FUV continuum power-law slope αν, FUV = −0.34 ± 0.03 consistent with previous results, but an EUV slope αν, EUV = −2.90 ± 0.04 that is much redder and inconsistent with any previous composite value (all ≳ −2.0). The EUV slope difference can be attributed in part to the tendency of previous studies to preferentially select UV brighter and bluer objects. The weak EUV flux suggests quasar accretion disc models that include outflows such as disc winds.
Evidence for planetary hypothesis for PTFO 8-8695 b with five-year optical/infrared monitoring observations
Authors: Tanimoto Yuta , Yamashita Takuya , Ui Takahiro , Uchiyama Mizuho , Kawabata Miho , Mori Hiroki , Nakaoka Tatsuya , Abe Taisei , Itoh Ryosuke , Kanda Yuka , Kawaguchi Kenji , Kawahara Naoki , Otsubo Ikki , Shiki Kensei , Takagi Kengo , Takaki Katsutoshi , Akitaya Hiroshi , Yamanaka Masayuki , Kawabata Koji S 
Published: February 2020

Abstract
PTFO 8-8695 b (CVSO 30 b) is a young planet candidate whose host star is a $\sim\!\!{2.6}$-Myr-old T-Tauri star, and there have been continuous discussions about the nature of this system. To unveil the mystery of this system, we observed PTFO 8-8695 for around five years at optical and infrared bands simultaneously using the Kanata telescope at the Higashi-Hiroshima Observatory. Through our observations, we found that the reported fading event split into two: deeper but phase-shifted “dip-A” and shallower but equiphase “dip-B”. These dips disappeared at different epochs, and then, dip-B reappeared. Based on the observed wavelength dependence of dip depths, a dust clump and a precessing planet are likely origins of dip-A and B, respectively. Here we propose “a precessing planet associated with a dust cloud” scenario for this system. This scenario is consistent with the reported change in the depth of fading events, and even with the reported results, which were thought to be evidence against the planetary hypothesis.
Family Standards of Living Over the Long Run, England 1280–1850*
Authors: Horrell Sara , Humphries Jane , Weisdorf Jacob 
Published: February 2020

Abstract
This article uses new wage series for men, women and children in combination with an established cost of living index and standard assumptions about family size to construct a measure of family welfare in England, 1280–1850. It asks whether this family could achieve a standard of living historically defined as ‘respectable’. It extracts information from primary and secondary sources to make adjustments for the participation rates of women and children, the varying number of days worked over time, the changing involvement of married women in paid work, and the evolving occupational structure. The resulting series is the first to depict the living standard of a representative working family over the very long run. Prior to the Black Death, this family existed just above subsistence; afterwards shortage of labour brought substantial albeit not unassailable gains. Tudor-era turmoil and constraints on women’s work pushed the family below the ‘respectable’ standard. From the mid 1600s however, the gradual transformation of the economy coincided with improved welfare. Over these centuries, it was rare for men’s work alone to sustain the family at a respectable level; women and children’s earnings were necessary. This article calls for a re-evaluation of the chronology, causes and consequences of long-run growth.