bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficacy and safety of two Ayurvedic dosage forms for Allergic rhinitis: Study protocol for an open-label randomized controlled trial
Authors: Dahanayake JM , Perera Pathirage Kamal , Galappaththy P , Samaranayake D 
Published: November 2019

Abstract
Background: Allergic rhinitis (AR) is an immune response of the nasal mucosa to airborne allergens and involves nasal congestion, watery nasal discharge, itching of the nose and sneezing. The symptoms of allergic rhinitis may significantly affect a patient’s quality of life and can be associated with conditions such as fatigue, headache, cognitive impairment and sleep disturbances. Various complementary and alternative medicine treatments have been used for this condition in clinical practice. The Ayurveda system of medicine is the most common complementary medicine system practiced in Sri Lanka. The aim of this study is to examine the efficacy and safety of a decoction used in traditional Ayurveda for allergic rhinitis and it’s ready to use freeze dried formulation in comparison to an antihistamine over a period of 4 weeks on relief of symptoms in allergic rhinitis. Study design: This is a three arm, open label, non-inferiority, randomized controlled clinical trial enrolling patients with AR. Tamalakyadi decoction containing 12 ingredients (TMD12), used in traditional Ayurveda and its freeze dried formulation are the test products. The efficacy and safety of the two Ayurvedic dosage forms will be tested against the antihistamine loratidine Patients with symptoms of AR will be allocated randomly into the 3 arms after a 1 week run-in period and the medications will be given orally for 28 days. Total Nasal symptom Score (TNSS) of the patients will be used as the primary efficacy endpoint. TNSS will be recorded and compared between the 3 arms prior to visit 1, at the end of 28 days, end of the and second months of follow up. Symptom scores of daytime nasal symptoms, night time nasal symptoms, non-nasal symptoms and Health Related Quality of Life questionnaire are used as secondary end points. Discussion: This clinical trial will be able to provide evidence based scientific data on Ayurvedic dosage form, TMD12 and the freeze-dried formulation in the treatment of allergic rhinitis. This trial is expected to develop capacity to scientifically evaluate various Ayurvedic treatments that are claimed to have efficacy in treatment of various disease conditions. Trial registration:ISRCTN18149439 (06 May 2019) Keywords: Allergic rhinitis, Ayurvedic dosage forms, Tamalakyadi decoctions, Randomized controlled trial
Optimised protocol of QIAamp® DNA mini Kit for bacteria genomic DNA extraction from both pure and mixture sample
Authors: Yang Zhong 
Published: November 2019

Abstract
As the sequencing technology developed, Next Generation Sequencing (NGS) has been used by more and more microbiology lab from different fields. Meanwhile , the quality of DNA will highly influence the sequencing result. Recently, there is an increasingly requirement for effective DNA extraction protocol to get high quality DNA from both pure and mixture cultures. Especially for researchers who what to study the microorganisms from complex mixture samples from food and environment. In this protocol, we modified the protocol of a commercial DNA extraction kit from Qiagen, and made it suitable for DNA extraction of different sample sources. The optimized protocol is proved to be effective. The DNA extracted with this protocol has been sent for NGS and get many interesting result.
Development of HealthRESPECT; An integrated service model for older long-term care hospital/nursing home patients using ICT
Authors: Choi Jung-Yeon , Kim Kwang-il , Lim Jae-Young , Ko Jin Young , Yoo Sooyoung , Kim Hongsoo , Lee Minho , Jang Sae-Kyun , Lee Dong Hee , Lee Jungwoo , Jung Young-il , Oh In-Hwan 
Published: November 2019

Abstract
Background: In Korea, fast aging country, an optimal healthcare service model for older adults should be developed. The purpose of this study is to identify the current healthcare system and to develop new healthcare service model using information and communication technologies (ICT) for older patients. Methods: Qualitative literature review, focus group interview and structured survey were conducted to identify current healthcare systems for older patients. Afterwards, we developed healthcare service model which include contests of comprehensive geriatric assessment, disease management, drug management, rehabilitation and consultation service using ICT. Result: Sixty (23 hypertension, 18 diabetes and 19 heart failure) articles are reviewed. ICT based chronic disease management models was effective and patients’ satisfaction were improved. In focus group interview and structured survey, current patients and medical information transfer between hospitals was inconvenient, costly and time consuming, especially in long-term care hospital (LTCH) and nursing home (NH) and the necessity of service model to manage older patients using ICT was confirmed. ICT based service model for older patients dwelling LTCH and NH was developed (Health-RESPECT) which include evidence based 1) comprehensive geriatric assessment (CGA) and establishment of customized management strategy 2) chronic disease management service with decision support system 3) potentially inappropriate medication management service 4) rehabilitation service and 5) consultation and videoconference service. Conclusion : This study is meaningful to identify current healthcare systems in older adults and to develop a system to manage older patient living in LTCH and NH using ICT. However, Health-RESPECT service model will need to be validated in further study.
Heterogeneity of Moraxella isolates found in the nasal cavities of piglets
Authors: López-Serrano Sergi , Galofré-Milà Nuria , Costa-Hurtado Mar , Pérez-de-Rozas Ana M. , Aragon Virginia 
Published: November 2019

Abstract
Previous studies have shown that the genus Moraxella is commonly present in the nasal microbiota of swine. In this study, 51 isolates of Moraxella were obtained from nasal swabs from 3-4 week old piglets, which represented 26 different fingerprintings by enterobacterial repetitive intergenic consensus (ERIC)-PCR. Whole 16S rRNA gene sequencing allowed the identification at species level of the Moraxella spp. isolates. The majority of the field strains were identified as Moraxella pluranimalium, but Moraxella porci was also detected. In addition, a cluster of 7 strains did not group with any described Moraxella species, probably representing a new species. Subsequent phenotypic characterization indicated that strains of Moraxella pluranimalium were mainly sensitive to the serum complement, while the cluster representing the putative new species was highly resistant. Biofilm formation capacity was very variable among the Moraxella spp. isolates, while adherence to epithelial cell lines was similar among selected strains. Additionally, selected strains were tested in phagocytosis assays and again variability was observed in the susceptibility to alveolar macrophages. Antimicrobial tests evidenced the existence of multidrug-resistance in the strains. In summary, phenotypic characterization revealed heterogeneity among Moraxella strains from the nasal cavity of piglets. Strains with pathogenic potential were detected as well as those that may be commensal members of the nasal microbiota. However, the role of Moraxella in porcine diseases and health should be further evaluated.
Parents' and caregivers' perceptions of the use of live video recording in neonatal units: A focus group study.
Authors: BRIS Aude LE , MAZILLE Nadia , SIMONOT Pauline , LUHERNE Maude , FLAMANT Cyril , GASCOIN Geraldine , Laighin Gearóid Ó , HARTE Richard , PLADYS Patrick 
Published: November 2019

Abstract
To study the perceptions of parents and caregivers on the use of live video in neonatal clinical practice in order to better assess its suitability, limits and constraints.Methods: Nine focus group interviews were conducted in four neonatal units involving 20 caregivers and 19 parents. Data were triangulated using transcripts and field notes and analyzed using inductive and semantic thematic analysis.Results: The seven major themes that emerged from the caregiver focus groups were (i) the impact of video recording on caregiver’s behavior; (ii) the impact on parents; (iii) forensic issues ;(iv) guarantee of use; (v) benefits for the new-born; (vi) methodology of use; and (vii) technical considerations & feasibility. The five major themes emerge from parents focus groups were i) benefits for the new-born and care enhancement; (ii) impact on parents and potential benefits in case of newborn child/parent separation; (iii) informed consent and guarantee of use;(iv) concern about a possible disruptive impact on caregivers; and (v) data protection.Conclusion: Both parents and caregivers found the use of video in care useful and acceptable if measures were taken to protect the data and mitigate any negative impacts on caregivers.
Safety and Effectiveness of Transvaginal Mesh Placement in Treating Pelvic Organ Prolapse
Authors: Chen Zeyu , Du Wensheng , Zhu Haitao 
Published: November 2019

Abstract
To observe the clinical effect and complications of transvaginal mesh (TVM) placement on treating pelvic organ prolapse (POP).Methods From May 2014 to June 2017, 89 patients with moderate to severe pelvic organ prolapse and pelvic floor defect (PFD) had received TVM pelvic reconstruction at our hospital. The operation time, intraoperative blood loss and intraoperative complications of all patients were recorded, and the incidence of postoperative complications was also observed. In addition, the POP-Q was utilized to evaluate the severity of POP in patients before surgery, as well as at 6 weeks, 6 months, 12 months and 24 months after surgery, respectively; besides, the Pelvic Floor Influence Questionnaire-7 (PFIQ-7), Pelvic Floor Dysfunction Inventory 20 (PFDI-20), and Patient Global Impression of Improvement (PGI-I) were adopted to evaluate the quality of life of patients.Results All the 89 cases had successfully completed the surgery, with the operation time of 60.0±13.0 min and the intraoperative blood loss of 57.2±21.9 mL. All patients had no bladder or rectal injury during the surgery. 2 patients displayed asymptomatic recurrence ,10 cases had postoperative fever, while 3 had mesh exposure, and all of them were improved after symptomatic treatment. Upon discharge, the lumbosacral and pubic discomfort, as well as pelvic organ dragging sensation had disappeared in all patients. In the final follow-up, the PFIQ-7 and PFDI-20 scores in the 89 cases were reduced compared with those before surgery (Ps<0.05).Conclusions TVM placement is effective on treating moderate to severe POP, which is associated with a lower complication rate and higher postoperative satisfaction.
Urinary angiotensinogen as a surrogate marker predicting the antiproteinuric effects of angiotensin receptor blockers in patients with overt proteinuria: a multicenter prospective study
Authors: Jeon Junseok , Kim Do Hee , Jang Hye Ryoun , Lee Jung Eun , Huh Wooseong , Kim Hye-Young , Kim Dae Joong , Kim Yoon-Goo 
Published: November 2019

Abstract
Although urinary angiotensinogen (AGT) and renin reflect intrarenal renin-angiotensin system activity and are enhanced in proteinuric chronic kidney disease, the clinical value of urinary AGT and renin levels during antiproteinuric treatment has yet to be determined. We investigated the clinical usefulness of initial urinary AGT or renin to determine the antiproteinuric effects of angiotensin receptor blockers (ARBs). Methods: This multicenter, prospective, single-arm study included 205 patients with overt proteinuria (urinary protein/creatinine ratio [uPCR] ≥ 1 mg/mg) enrolled between April 2009 and December 2011. All patients were treated with valsartan. The urinary AGT/creatinine ratio (uAGT/Cr) was measured at the baseline and 24 weeks, and the renin/creatinine ratio (uR/Cr) was measured at the baseline. Fifty-six patients were followed-up for 5 years. Results: The mean age was 47.6 years and 51.2% were male. The mean uPCR was 2.32 mg/mg and the mean eGFR was 63.2 mL/min/1.73m 2 . Natural logarithms (ln) (uAGT/Cr), ln(uR/Cr), and diabetes mellitus were associated with proteinuria decrement (decrease in uPCR ≥ 1 mg/mg). Ln(uAGT/Cr) was an independent predictor for proteinuria decrement (OR 1.372, 95% CI, 1.068–1.762, P = 0.013). Among the 56 patients followed-up for 5 years, Δln(uAGT/Cr) at 24 weeks was an independent predictor for uPCR < 1 mg/mg at 5 years (OR 0.379, 95% CI, 0.20–0.715, P = 0.003). Conclusions: Our study demonstrates the potential role of both baseline urinary AGT and changes in urinary AGT during the initial 24 weeks as surrogate markers predicting the antiproteinuric effects of ARBs in patients with overt proteinuria.
CUTseq is a versatile method for preparing multiplexed DNA sequencing libraries from low-input samples
Authors: Zhang Xiaolu , Garnerone Silvano , Simonetti Michele , Harbers Luuk , Nicoś Marcin , Mirzazadeh Reza , Venesio Tiziana , Sapino Anna , Hartman Johan , Marchiò Caterina , Bienko Magda , Crosetto Nicola 
Published: November 2019

Abstract
Current multiplexing strategies for massively parallel sequencing of genomic DNA mainly rely on library indexing in the final steps of library preparation. This procedure is costly and time-consuming because a single library must be produced separately for each sample. Furthermore, library preparation is challenging in the case of low-input fixed samples, such as DNA extracted from formalin-fixed paraffin-embedded (FFPE) tissues. Here, we describe CUTseq, a method that uses restriction enzymes and in vitro transcription to barcode and amplify genomic DNA prior to library construction. We thoroughly validate CUTseq and demonstrate its applicability to both genome and exome sequencing, enabling multi-region genome profiling within single stained FFPE tissue sections, to assess intratumor heterogeneity at high spatial resolution. In conclusion, CUTseq is a versatile and cost-effective method for multiplexed DNA sequencing library preparation that can find numerous applications in research and diagnostics.
DNA methylation and miRNA-1296 act in concert to mediate spatiotemporal expression of KPNA7 during bovine oocyte and early embryonic development
Authors: Wang Lei , Hand Jacqelyn M. , Fu Liyuan , Smith George W. , Yao Jianbo 
Published: November 2019

Abstract
Background Epigenetic regulation of oocyte-specific maternal factors is essential for oocyte and early embryonic development. KPNA7 is an oocyte-specific maternal factor, which controls transportation of nuclear proteins important for early embryonic development. To elucidate the epigenetic mechanisms involved in the controlled expression of KPNA7, both DNA methylation associated transcriptional silencing and miRNA-mediated mRNA degradation of KPNA7 were examined. Results Comparison of DNA methylation profiles at the proximal promoter of KPNA7 gene between oocyte and 6 different somatic tissues identified 3 oocyte-specific differentially methylated CpG sites. Expression of KPNA7 mRNA was reintroduced in bovine kidney-derived CCL2 cells after treatment with the methylation inhibitor, 5-aza-2-deoxycytidine (5-Aza). Analysis of the promoter region of KPNA7 gene in CCL2 cells treated with 5-Aza showed a lighter methylation rate in all the CpG sites. Bioinformatic analysis predicted 4 miRNA-1296 binding sites in the coding region of KPNA7 mRNA. Ectopic co-expression of miRNA-1296 and KPNA7 in HEK293 cells led to reduced expression of KPNA7 protein. Quantitative real time PCR analysis revealed that miRNA-1296 is expressed in oocytes and early stage embryos, and the expression reaches a peak level in 8-cell stage embryos, coincident with the time of embryonic genome activation and the start of declining of KPNA7 expression. Conclusions These results suggest that DNA methylation may account for oocyte-specific expression of KPNA7, and miRNA-1296 targeting the coding region of KPNA7 is a potential mechanism for KPNA7 transcript degradation during the maternal-to-zygotic transition.
Electrochemical oxidation as a post treatment for biologically tannery wastewater in batch reactor
Authors: Le Luu Tran , Stephane Djeuga Djeuga Franck , Minh Nguyen Hoang , Canh Nguyen Duc , Thanh Bui Xuan 
Published: November 2019

Abstract
Tannery wastewater is known to contain high concentrations of organic compounds, heavy metals, nitrogen, sulphur, chromium, and many other chemicals. Both aerobic and anaerobic biological approaches have proven ineffective in the treatment of tannery wastewater due to the high salinity and toxic chemicals contained within the medium. Electrochemical oxidation presents a promising method for solving this problem. High pollutant removal efficiency, low energy consumption, and high electrode stability are three important factors supporting the feasibility of an efficient electrochemical treatment process. In the present study, electrochemical oxidation was performed as a post treatment for tannery wastewater (after biological pre-treatment) in a batch reactor using Ti/RuO2, Ti/IrO2, and Ti/BDD anodes. The effects of pH, current density, stirring rate and treatment time were studied to assess the treatment efficiency as well as the energy consumption of the process. The results showed that colour, chemical oxygen demand (COD), total organic carbon (TOC), and total nitrogen (TN) removal efficiencies on the electrodes were: Ti/RuO2 (88.8%, 88.40%, 64.0%, 96.4%), Ti/IrO2 (85.40%, 85.9%, 52.3%, 51.4%), Ti/BDD (90.60%, 94.7%, 90.5%, 82.7%) respectively, at a current density of 80 mA/cm2. All three electrodes demonstrated optimal performance at a pH of 8, a stirring rate of 400 rpm, a current density of 80 mA/cm2, and an electrolysis time of 5 h. The concentration of tri-chloromethane by-product was detected with limiting value. Electrochemical oxidation thus offers a feasible method for removing organic compounds and nutrients from tannery wastewater.
Purification, Biochemical Characterization and Decolorization Efficiency of Laccases from Peach and Cherry Cultures of Pleutorus eryngii: A Comparative Study
Authors: Akpinar Merve , Urek Raziye Ozturk 
Published: November 2019

Background::
Laccases (Lacs) are used potentially in industrial and biotechnological
applications such as decolorization of dyes, degradation of industrial effluents, delignification, etc.
thanks to their large varieties of substrate specificities and excellent catalytic efficiencies. The
efficient utilizations of Lacs in these applications mostly depend on the identifying their
biochemical properties.

Objective:
The goal of this research is to investigate the purification, biochemical characterization
and decolorization efficiencies of Lacs.

Methods:
Pleurotus eryngii was incubated on peach (PC) and cherry (CC) wastes under optimized
solid state fermentation conditions. Then, the enzymes extracts were purified by ammonium sulfate
precipitation, anion exchange chromatography, gel filtration, respectively. Lacs fractions were
subjected to electrophoretic analyses as well as their structural and kinetic characteristics. Also, the
effects of selected chemical agents on purified Lacs activities and determination of decolorization
efficiencies were studied.

Results:
As the results of purification processes of Lacs from both cultures, 3.94-fold purification
was obtained for PC, while it was 5.34 for CC. The electrophoretic results of purified Lacs
illustrated the single bands of protein (30±1 kDa) in accordance with the results after gel filtration.
The Km values of Lacs from PC and CC were respectively detected as 1.1381 and 0.329 mM for
ABTS. The selected agents partially/completely inhibited Lac activities. The highest decolorization
efficiencies of purified Lacs from PC and CC were separately obtained as 53 and 11.8%.

Conclusion:
The results clearly indicated that the performances of Lacs from both cultures in
decolorization application are different from each other depending their activities, biochemical and
kinetic characteristics.
Wnt-β-catenin Signaling Pathway, the Achilles' Heels of Cancer Multidrug Resistance
Authors: Ghandadi Morteza , Valadan Reza , Mohammadi Hamidreza , Akhtari Javad , Khodashenas Shabanali , Ashari Sorour 
Published: November 2019

Background:Most of the anticancer chemotherapies are hampered via the development of multidrug resistance (MDR), which is the resistance of tumor cells against cytotoxic effects of multiple chemotherapeutic agents. Overexpression and/or over-activation of ATP-dependent drug efflux transporters is a key mechanism underlying MDR development. Moreover, enhancement of drug metabolism, changes in drug targets and aberrant activation of the main signaling pathways, including Wnt, Akt and NF-κB are also responsible for MDR.Methods:In this study, we have reviewed the roles of Wnt signaling in MDR as well as its potential therapeutic significance. Pubmed and Scopus have been searched using Wnt, β-catenin, cancer, MDR and multidrug resistance as keywords. The last search was done in March 2019. Manuscripts investigating the roles of Wnt signaling in MDR or studying the modulation of MDR through the inhibition of Wnt signaling have been involved in the study. The main focus of the manuscript is regulation of MDR related transporters by canonical Wnt signaling pathway.Result and Conclusion:Wnt signaling has been involved in several pathophysiological states, including carcinogenesis and embryonic development. Wnt signaling is linked to various aspects of MDR including P-glycoprotein and multidrug resistance protein 1 regulation through its canonical pathways. Aberrant activation of Wnt/β- catenin signaling leads to the induction of cancer MDR mainly through the overexpression and/or over-activation of MDR related transporters. Accordingly, Wnt/β-catenin signaling can be a potential target for modulating cancer MDR.
Pharmacokinetic Evaluation of 99mTc-radiolabeled Solid Lipid Nanoparticles and Chitosan Coated Solid Lipid Nanoparticles
Authors: Gharibkandi Nasrin Abbasi , Molavipordanjani Sajjad , Akbari Jafar , Hosseinimehr Seyed Jalal 
Published: November 2019

Background:
Solid Lipid Nanoparticles (SLNs) possess unique in vivo features such as high resistivity,
bioavailability, and habitation at the target site. Coating nanoparticles with polymers such as chitosan greatly affects
their pharmacokinetic behavior, stability, tissue uptake, and controlled drug delivery. The aim of this study was to
prepare and evaluate the biodistribution of 99mTc-labeled SLNs and chitosan modified SLNs in mice.

Methods:
99mTc-oxine was prepared and utilized to radiolabel pre-papered SLNs or chitosan coated SLNs. After
purification of radiolabeled SLNs (99mTc-SLNs) and radiolabeled chitosan-coated SLNs (99mTc-Chi-SLNs) using
Amicon filter, they were injected into BALB/c mice to evaluate their biodistribution patterns. In addition, nanoparticles
were characterized using Transmission Electron Microscopy (TEM), Fourier-transform Infrared Spectroscopy
(FTIR), Differential Scanning Calorimetry (DSC), X-ray Powder Diffraction (XRD) and Dynamic Light Scattering
(DLS).

Results:
99mTc-oxine with high radiochemical purity (RCP~100%) and stability (RCP > 97% at 24 h) was used to
provide 99mTc-SLNs and 99mTc-Chi-SLNs with high initial RCP (100%). TEM image and DLS data suggest 99mTc-
SLNs susceptibility to aggregation. To that end, the main portion of 99mTc-SLNs radioactivity accumulates in the
liver and intestines, while 99mTc-Chi-SLNs sequesters in the liver, intestines and kidneys. The blood radioactivity of
99mTc-Chi-SLNs was higher than that of 99mTc-SLNs by 7.5, 3.17 and 3.5 folds at 1, 4 and 8 h post-injection. 99mTc-
Chi-SLNs uptake in the kidneys in comparison with 99mTc-SLNs was higher by 37.48, 5.84 and 11 folds at 1, 4 and
8h.

Conclusion:
The chitosan layer on the surface of 99mTc-Chi-SLNs reduces lipophilicity in comparison with 99mTc-
SLNs. Therefore, 99mTc-Chi-SLNs are less susceptible to aggregation, which leads to their lower liver uptake and
higher kidney uptake and blood concentration.