bluuebunny/crossref_metadata_2025_split huggingface dataset:
RIOK2 is negatively regulated by miR‐4744 and promotes glioma cell migration/invasion through epithelial‐mesenchymal transition
Authors: Song Yunnong , Li Cheng , Jin Lei , Xing Jingsong , Sha Zhuang , Zhang Tong , Ji Daofei , Yu Rutong , Gao Shangfeng 
Published: March 2020

AbstractRIOK2 is a member of RIO (right open reading frame) kinase family. Recent studies have revealed the involvement of RIO kinases in glioma cell growth and expansion. However, the role and mechanism of RIOK2 in glioma cell migration and invasion remain unclear. Wound healing assay, Transwell assay and real‐time quantitative PCR (qRT‐PCR) detection of matrix metalloproteinases (MMPs) were used to evaluate the migration/invasion of glioma cells. Western blot and qRT‐PCR were employed to measure the expression of epithelial‐mesenchymal transition (EMT) markers. Dual luciferase reporter assay was performed to determine the binding between RIOK2 and miR‐4744. In addition, RIOK2 and miR‐4744 levels were quantified by qRT‐PCR and/or immunohistochemistry in glioma tissues. Transfection of RIOK2 siRNAs significantly inhibited glioma cell migration and invasion and down‐regulated the expression of MMPs (MMP2 and MMP9) and mesenchymal markers (N‐cadherin, β‐catenin, Twist1, fibronectin, ZEB‐1) in glioma cells. Overexpression of RIOK2 showed the opposite effects. MiR‐4744 directly bound to the 3'‐untranslated region of RIOK2 and negatively regulated the expression of RIOK2. Up‐regulation of miR‐4744 inhibited the migration and invasion of glioma cells. Overexpression of RIOK2 could reverse the effects of miR‐4744 up‐regulation on the migration, invasion and EMT process in glioma cells. Moreover, RIOK2 was high, while miR‐4744 was low in glioma tissues, and a negative correlation was found between them. These results suggest that RIOK2 is post‐transcriptionally targeted by miR‐4744, the low miR‐4744 and high RIOK2 levels in glioma may contribute to tumour cell infiltration through promoting the EMT.
Obtaining optimal esthetics with veneered zirconia and lithium disilicate frameworks on substrates of different colors
Authors: Spazzin Aloísio O. , Schestatsky Ricardo , Ness Carla R. B. , Alessandretti Rodrigo , Pereira Gabriel K. R. 
Published: March 2020

AbstractObjectiveThis study sought to describe the clinical and laboratory procedures and to discuss aspects involved in obtaining satisfactory esthetics on substrates presenting different colors.Clinical considerationsThis case report describes the clinical and laboratory procedures for obtaining an optimal esthetic result using different ceramic framework materials veneered with feldspathic ceramic over substrates of different colors. Zirconia frameworks were used for darkened substrates and were reinforced with lithium disilicate for substrates with minimal discoloration. The same feldspathic veneering ceramic was applied to provide similar translucency and color for favorable esthetics.ConclusionsThe materials explored in the present case report presented favorable aesthetic results in masking darkened substrates.Clinical significanceClinicians should consider the condition and color of the substrate when selecting the restorative material for masking such a condition.
Development of an approach to evaluate the failure probabilities of river levees based on expert judgement: Application to a case study
Authors: Hathout Michel , Peyras Laurent , Carvajal Claudio , Diab Youssef , Vuillet Marc 
Published: March 2020

AbstractStudies carried out to analyse the risks of levees must include an evaluation of the probabilities of occurrence of different failure mechanisms. The probabilistic quantitative evaluation of these mechanisms remains difficult due to often insufficient data, the natural variability of the materials, structures that are very long, and the unavailability of mechanical models for certain failure mechanisms. This makes it necessary to call for expert judgement to evaluate the probabilities of failure. However, expert judgement generally has qualitative and subjective dimensions, and it includes biases that are liable to impair the capacities of an expert to elicit their evaluations. This article proposes an approach to processing expert judgement that includes the modalities ofIndividualexpertElicitation,Calibration,Aggregation, andDebiasing of expert judgement (IeCAD). This IeCAD approach has been developed for river levees in view to correcting biased expert evaluations in the case of evaluating the failure probability of structures.
In vitro antimicrobial effects of Myristica fragrans essential oil on foodborne pathogens and its influence on beef quality during refrigerated storage
Authors: Kiarsi Zahra , Hojjati Mohammad , Behbahani Behrooz Alizadeh , Noshad Mohammad 
Published: March 2020

AbstractNutmeg (Myristica fragrans) seed was subjected to the hydro‐distillation method to extract its essential oil (NEO). Its main constituents were α‐pinene (20.16%), sabinene (14.45%), and β‐pinene (13.26%) with great antimicrobial and antioxidant actions. A novel edible coating was then fabricated based on the sage seed mucilage (SSM) and NEO, to ameliorate the quality and shelf‐life of beef slices. The NEO‐loaded SSM coating was able to significantly decrease the population of total viable count, Escherichia coli, Staphylococcus aureus, psychrotrophic bacteria, and fungi. Moreover, lipid oxidation of beef slices was remarkably suppressed upon the application of bioactive NEO‐loaded SSM edible coating, as compared with the control by Day 6. The coated beef sample, especially NEO‐rich SSM coated ones perceived a higher consumer acceptance and firmness than the SSM coated and noncoated samples. The SSM edible coating containing NEO could therefore have the potential to control the growth of pathogenic microorganisms and lipid oxidation, and to improve the color stability and sensory properties of meat and meat products.
Umbilical Cord Practices of Members of the American College of Nurse‐Midwives
Authors: Leslie Mayri Sagady , Erickson‐Owens Debra , Park Jeongyoung 
Published: March 2020

IntroductionMercer et al surveyed members of the American College of Nurse‐Midwives (ACNM) about their umbilical cord clamping practices in 2000. Over the last 20 years, a significant body of research supporting delayed cord clamping (DCC) has been published. The purpose of this study was to learn how midwives today manage the umbilical cord at birth.MethodsTo better understand the current practices of midwives, in 2017, a national online survey of ACNM members was conducted. A total of 24 questions were asked about DCC, cord milking, specific clinical circumstances, and the presence of policies or guidelines.ResultsA total of 5306 surveys were sent with 1106 responses. After applying inclusion criteria, 1050 were available for analysis. Respondents practiced in all settings: home, birth centers, and hospitals. Compared with 2000, a 46% increase in the practice of DCC was identified. In this study, 98% of the participants reported facilitating DCC for full‐term vaginal births as compared with 67% in 2000. In addition, 25% practiced DCC for near‐term and 65% for preterm neonates. Cord milking was practiced by 37% of participants. When asked about barriers to practicing DCC, 54% of participants identified time pressures to hand off the newborn as the greatest detriment. It was challenging to practice DCC in situations wherein the newborn needed resuscitation or in breech births. Far fewer midwives practice cord milking compared with DCC.DiscussionThe survey results suggest there has been an increase in the practice of DCC over the last 20 years. Cord milking is not as widely practiced as DCC, and respondents were less likely to be convinced by the evidence for cord milking. This speaks to the opportunity for more education for midwives. There is also a need for clinical guidelines that address umbilical cord management when challenging circumstances arise such as breech birth, shoulder dystocia, and the need for resuscitation.
Do CEOs Matter? Evidence from Hospitalization Events
Authors: BENNEDSEN MORTEN , PÉREZ‐GONZÁLEZ FRANCISCO , WOLFENZON DANIEL 
Published: March 2020

ABSTRACTUsing variation in firms’ exposure to their CEOs resulting from hospitalization, we estimate the effect of chief executive officers (CEOs) on firm policies, holding firm‐CEO matches constant. We document three main findings. First, CEOs have a significant effect on profitability and investment. Second, CEO effects are larger for younger CEOs, in growing and family‐controlled firms, and in human‐capital‐intensive industries. Third, CEOs are unique: the hospitalization of other senior executives does not have similar effects on the performance. Overall, our findings demonstrate that CEOs are a key driver of firm performance, which suggests that CEO contingency plans are valuable.
Sexual Orientation, Geographic Proximity, and Contact Frequency Between Adult Siblings
Authors: Perales Francisco , Plage Stefanie 
Published: March 2020

ObjectiveThe aim of this study is to provide the first systematic analysis of differences in the closeness of sibling relations between lesbian, gay, and bisexual (LGB) and heterosexual adults.BackgroundAcross countries, LGB people experience poorer socioeconomic and health outcomes than heterosexual people. Although the reasons for this disadvantage are complex, low levels of family support have been recurrently identified as an important contributor. However, the available literature has concentrated on relations between LGB people and their parents, neglecting other important familial bonds—including sibling bonds.MethodTo compare the closeness of sibling relations between individuals with different sexual orientations, this study leverages unique data from an Australian national survey (Household, Income and Labour Dynamics in Australia Survey, n = 13,157 individuals, 35,622 individual‐sibling pairs) and multilevel regression models.ResultsKey results indicated that—when compared with heterosexual individuals—LGB individuals had less frequent contact with and lived geographically farther from their siblings. The pattern of effects was similar for bisexual and gay or lesbian individuals, and stronger for male than female sexual‐minority individuals. There was only sparse evidence of moderation of these associations by individual or sibling traits.ConclusionsThe findings of this study are consistent with theoretical perspectives highlighting the unique barriers to socioeconomic inclusion experienced by individuals from sexual minorities. They suggest that these barriers begin within the nuclear family.
Why do supervisors abuse subordinates? Effects of team performance, regulatory focus, and emotional exhaustion
Authors: Fan Xue‐Ling , Wang Qi‐Qi , Liu Jun , Liu Chao , Cai Tao 
Published: March 2020

Complementing previous studies on the antecedents of abusive supervision at the organizational and individual level, this study investigates how a team‐level factor (i.e., team performance) influences abusive supervision. Drawing on the conservation of resources theory, we propose that low team performance may elicit abusive supervision by making supervisors emotionally exhausted. Furthermore, when faced with low team performance, supervisors with a high prevention focus may experience greater emotional exhaustion and thus display more abusive behaviours, while supervisors with a high promotion focus may be less likely to feel emotionally exhausted and thereby exhibit fewer abusive behaviours. We tested our hypotheses using three‐wave survey data collected from 130 teams within an organization. Findings showed that supervisors' emotional exhaustion mediated the negative effect of team performance on abusive supervision, while only prevention focus moderated the indirect effect of team performance on abusive supervision via emotional exhaustion. This study contributes to the literature on antecedents of abusive supervision, the relationship between abusive supervision and performance, and leadership literature broadly.Practitioner points

As abusive supervision results from psychological stress in a specific form of emotional exhaustion, organizations need to improve supervisors’ stress‐management skills by designing relevant training or education programmes, and help them handle or recover from stress.

The study’s identification of the specific stressor (i.e., low team performance) fostering abusive supervision can increase organizations’ awareness of the issue and enable them to take appropriate interventions to reduce abusive supervision.

Confronted with low team performance, highly prevention‐focused supervisors become more emotionally exhausted and are therefore more likely to abuse subordinates. Thus, in organizations where such a stressor (i.e., low team performance) is salient, practitioners can hire supervisors with a low prevention focus, or assess, select, and promote supervisors based on their prevention focus.
Validation of a web‐based version of the Craniofacial Pain and Disability Inventory
Authors: Li Xiao‐ling , He Song‐lin , Wang Jin‐hua 
Published: March 2020

AbstractObjectiveTo translate and cross‐culturally adapt the Craniofacial Pain and Disability Inventory (CF‐PDI) into Chinese and, to evaluate measurement properties of the web‐based version of the CF‐PDI.MethodsThe Chinese version (CF‐PDI/C) was first produced according the guidelines. Then, the web‐based version of CF‐PDI/C (eCF‐PDI/C) was developed by our team and a third‐party survey provider. The eCF‐PDI/C was distributed to patients with painful temporomandibular disorders (TMD) with or without headache to evaluate its psychometric properties. The reliability of the eCF‐PDI/C was detected by internal consistency and test‐retest reliability. The validity of the measure was performed through construct validity and convergent validity.ResultsA total of 206 patients were recruited. The Cronbach's α value and the intraclass correlation coefficients (ICC) value of the eCF‐PDI/C was .912 and .82, respectively. Following exploratory factor analysis (EFA), three factors were extracted, accounting for 77.153% of the total variation. With regard to the convergent validity, the measure evidenced a good relationship with the global health question.ConclusionsThe eCF‐PDI/C displays good reliability and validity through rigorous performance tests. This can be recommended for use among patients with painful TMD with or without headache.
Food Allergy Management at School
Authors: Dupuis Roxanne , Kinsey Eliza Whiteman , Spergel Jonathan M. , Brown‐Whitehorn Terri , Graves Amy , Samuelson Kate , Epstein Caleb , Mollen Cynthia , Cannuscio Carolyn C. 
Published: March 2020

ABSTRACTBACKGROUNDApproximately 8% of schoolchildren in the United States experience potentially life‐threatening food allergies. They must diligently avoid allergenic foods and have prompt access to epinephrine to treat anaphylaxis. These prevention strategies must be sustained without interruption, posing a range of challenges at school.METHODSWe conducted semi‐structured interviews with 178 participants about their experiences managing food allergies outside the home. Interviews were transcribed and analyzed using an iterative approach in NVivo 10.RESULTSParticipants reported highly varied school experiences across the ecological model. They described the need to be proactive and self‐sufficient to manage food allergies. Whereas food allergy‐related social exclusion was common, participants also described positive peer interactions, including intensive peer engagement and support. They perceived that formal school policies were limited in scope and inconsistently implemented. Prevention‐oriented policies were more common in lower grades than in higher grades.CONCLUSIONSPoorly defined and implemented policies disrupted students' social and educational experiences at school, families' relationships with school staff, and, ultimately, the safety and wellbeing of students with allergies. Given the high prevalence of food allergies among children, these findings demonstrate the need for multiple layers of support to facilitate safe, socially inclusive food allergy management at schools.
Undergoing colonoscopy as experienced by adolescents
Authors: Vejzovic Vedrana , Örmon Karin 
Published: March 2020

AbstractPurposeThe aim of this study was to describe the phenomenon of undergoing colonoscopy as experienced by adolescents.DesignThis study was a qualitative study in which data were collected and analyzed in accordance with the methodological principles of Reflective Lifeworld Research with a phenomenological approach.MethodsFace‐to‐face interviews were performed with 17 adolescents after undergoing the first colonoscopy.ResultsThe phenomenon of undergoing colonoscopy as experienced by adolescents can be described as a collision between emotions and a desire to obtain answers to questions about the examination, as well as concerns about its result and the meaning of undergoing colonoscopy. The essential meaning is additionally described through its constituents: a sense of vulnerability, an opportunity for symptom explanation, and sensibility regarding information.ConclusionsThe results can be concluded in terms of the knowledge that for adolescents a colonoscopy means more than an examination. Although colonoscopy is not experienced as painful, it evokes different emotions that affect adolescents. Therefore, a psychological preparation, on an individual level, is required before the colonoscopy. Our results showed that adolescents need to understand the connection between their symptoms, their body, and the colonoscopy.
PNPLA3I148M gene variant and chronic kidney disease in type 2 diabetic patients with NAFLD: Clinical and experimental findings
Authors: Mantovani Alessandro , Taliento Alice , Zusi Chiara , Baselli Guido , Prati Daniele , Granata Simona , Zaza Gianluigi , Colecchia Antonio , Maffeis Claudio , Byrne Christopher D. , Valenti Luca , Targher Giovanni 
Published: March 2020

AbstractBackground and AimsEmerging evidence suggests an association between patatin‐like phospholipase domain‐containing protein‐3 (PNPLA3) rs738409 (I148M protein variant) and risk of chronic kidney disease (CKD), but the mechanisms underpinning this association are poorly understood.MethodsWe studied 157 patients with type 2 diabetes (T2DM) who underwent ultrasonography and vibration‐controlled transient elastography for diagnosing nonalcoholic fatty liver disease (NAFLD). CKD was defined as estimated glomerular filtration rate (e‐GFR) <60 mL/min/1.73 m2and/or abnormal albuminuria. We surveyedPNPLA3mRNA expression in human tissues, using the liver as a positive control, and also measuredPNPLA3mRNA and protein expression levels in human cell lines represented in the kidney and the liver.ResultsIn all, 112 patients had NAFLD and 43 had CKD. Patients homozygous for the I148M variant (n = 11) had lower e‐GFR levels (60.6 ± 11.7 vs 77.8 ± 15.9 vs 83.5 ± 16.5 mL/min/1.73 m2,P = .0001) and higher prevalence of CKD (63.6% vs 24.2% vs 25.0%,P = .028), compared to those with I/M (n = 66) and I/I (n = 80)PNPLA3genotype. The association of I148M homozygosity with lower e‐GFR levels (P < .0001) and higher risk of CKD (adjusted‐odds ratio 6.65; 95% CI 1.65‐26.8,P = .008) was independent of liver disease severity (as detected by liver stiffness ≥7kPa) and other risk factors.PNPLA3mRNA expression was greatest in liver and renal cortex, and podocytes showed high PNPLA3 mRNA and protein levels, comparable to that of hepatocytes and hepatic stellate cells respectively.ConclusionsThePNPLA3I148M variant was associated with CKD, independently of common renal risk factors and severity of NAFLDPNPLA3expression levels were particularly high in renal podocytes.
Fluconazole‐resistant Candida parapsilosis strains with a Y132F substitution in the ERG11 gene causing invasive infections in a neonatal unit, South Africa
Authors: Magobo Rindidzani E. , Lockhart Shawn R. , Govender Nelesh P. 
Published: March 2020

SummaryIntroductionThe prevalence of azole resistance in C parapsilosis is very low in most parts of the world. However, South Africa has reported an exceptionally high prevalence of azole resistance in C parapsilosis strains isolated from candidaemia cases. We aimed to determine the possible molecular mechanisms of fluconazole resistance in C parapsilosis isolates obtained through surveillance at a large neonatal unit at a South African academic hospital.MethodsWe sequenced the ERG11 and MRR1 genes of C parapsilosis isolates recovered from cases of neonatal candidemia, followed by microsatellite genotyping. A total of 73 isolates with antifungal susceptibility results were analysed.ResultsOf these, 57 (78%) were resistant, 11 (15%) susceptible dose‐dependent and 5 (7%) susceptible. The most commonly identified amino acid substitution within the ERG11 gene was Y132F in 68% (39/57) of fluconazole‐resistant isolates and none in susceptible isolates. Three amino acid substitutions (R405K, G583R and A619V) and 1 nucleotide deletion at position 1331 were identified within MRR1 gene in 19 (26%) isolates. Microsatellite genotyping grouped isolates into four clusters (50 isolates). Cluster 1 accounted for 23% (17/73) of all cases, cluster 2 for 22% (16/73), cluster 3 for 14% (10/73) and cluster 4 for 10% (7/73). We found an association between cluster type and fluconazole resistance (P‐value = .004). Isolates harbouring the Y132F substitution were more likely to belong to a cluster than non‐Y132F isolates.ConclusionFluconazole resistance in C parapsilosis strains from a single South African neonatal unit was associated with cluster type and predominantly driven by Y123F amino acid substitutions in the ERG11 gene.
The cyclic motor patterns in the human colon
Authors: Pervez Maham , Ratcliffe Elyanne , Parsons Sean P. , Chen Ji‐Hong , Huizinga Jan D. 
Published: March 2020

AbstractBackgroundHigh‐resolution colonic manometry gives an unprecedented window into motor patterns of the human colon. Our objective was to characterize motor activities throughout the entire colon that possessed persistent rhythmicity and spanning at least 5 cm.MethodsHigh‐resolution colonic manometry using an 84‐channel water‐perfused catheter was performed in 19 healthy volunteers. Rhythmic activity was assessed during baseline, proximal balloon distention, meal, and bisacodyl administration.Key ResultsThroughout the entire colon, a cyclic motor pattern occurred either in isolation or following a high‐amplitude propagating pressure wave (HAPW), consisting of clusters of pressure waves at a frequency centered on 11‐13 cycles/min, unrelated to breathing. The cluster duration was 1‐6 minutes; the pressure waves traveled for 8‐27 cm, lasting 5‐8 seconds. The clusters itself could be rhythmic at 0.5‐2 cpm. The propagation direction of the individual pressure waves was mixed with >50% occurring simultaneous. This high‐frequency cyclic motor pattern co‐existed with the well‐known low‐frequency cyclic motor pattern centered on 3‐4 cpm. In the rectum, the low‐frequency cyclic motor pattern dominated, propagating predominantly in retrograde direction. Proximal balloon distention, a meal and bisacodyl administration induced HAPWs followed by cyclic motor patterns.Conclusions and InferencesWithin cyclic motor patterns, retrograde propagating, low‐frequency pressure waves dominate in the rectum, likely keeping the rectum empty; and mixed propagation, high‐frequency pressure waves dominate in the colon, likely promoting absorption and storage, hence contributing to continence. Propagation and frequency characteristics are likely determined by network properties of the interstitial cells of Cajal.