bluuebunny/crossref_metadata_2025_split huggingface dataset:
Voltage- and time-dependent valence state transition in cobalt oxide catalysts during the oxygen evolution reaction
Authors: Zhou Jing , Zhang Linjuan , Huang Yu-Cheng , Dong Chung-Li , Lin Hong-Ji , Chen Chien-Te , Tjeng L. H. , Hu Zhiwei 
Published: April 2020

AbstractThe ability to determine the electronic structure of catalysts during electrochemical reactions is highly important for identification of the active sites and the reaction mechanism. Here we successfully applied soft X-ray spectroscopy to follow in operando the valence and spin state of the Co ions in Li2Co2O4 under oxygen evolution reaction (OER) conditions. We have observed that a substantial fraction of the Co ions undergo a voltage-dependent and time-dependent valence state transition from Co3+ to Co4+ accompanied by spontaneous delithiation, whereas the edge-shared Co–O network and spin state of the Co ions remain unchanged. Density functional theory calculations indicate that the highly oxidized Co4+ site, rather than the Co3+ site or the oxygen vacancy site, is mainly responsible for the high OER activity.
A vaccine-based nanosystem for initiating innate immunity and improving tumor immunotherapy
Authors: Zheng Di-Wei , Gao Fan , Cheng Qian , Bao Peng , Dong Xue , Fan Jin-Xuan , Song Wen , Zeng Xuan , Cheng Si-Xue , Zhang Xian-Zheng 
Published: April 2020

AbstractThe unsatisfactory response rate of immune checkpoint blockade (ICB) immunotherapy severely limits its clinical application as a tumor therapy. Here, we generate a vaccine-based nanosystem by integrating siRNA for Cd274 into the commercial human papillomavirus (HPV) L1 (HPV16 L1) protein. This nanosystem has good biosafety and enhances the therapeutic response rate of anti-tumor immunotherapy. The HPV16 L1 protein activates innate immunity through the type I interferon pathway and exhibits an efficient anti-cancer effect when cooperating with ICB therapy. For both resectable and unresectable breast tumors, the nanosystem decreases 71% tumor recurrence and extends progression-free survival by 67%. Most importantly, the nanosystem successfully induces high response rates in various genetically modified breast cancer models with different antigen loads. The strong immune stimulation elicited by this vaccine-based nanosystem might constitute an approach to significantly improve current ICB immunotherapy.
Synthesis of high-entropy alloy nanoparticles on supports by the fast moving bed pyrolysis
Authors: Gao Shaojie , Hao Shaoyun , Huang Zhennan , Yuan Yifei , Han Song , Lei Lecheng , Zhang Xingwang , Shahbazian-Yassar Reza , Lu Jun 
Published: April 2020

AbstractHigh-entropy alloy nanoparticles (HEA-NPs) are important class of materials with significant technological potential. However, the strategies for synthesizing uniformly dispersed HEA-NPs on granular supports such as carbon materials, γ-Al2O3, and zeolite, which is vital to their practical applications, are largely unexplored. Herein, we present a fast moving bed pyrolysis strategy to immobilize HEA-NPs on granular supports with a narrow size distribution of 2 nm up to denary (MnCoNiCuRhPdSnIrPtAu) HEA-NPs at 923 K. Fast moving bed pyrolysis strategy ensures the mixed metal precursors rapidly and simultaneously pyrolyzed at high temperatures, resulting in nuclei with a small size. The representative quinary (FeCoPdIrPt) HEA-NPs exhibit high stability (150 h) toward hydrogen evolution reaction with high mass activity, which is 26 times higher than the commercial Pt/C at an overpotential of 100 mV. Our strategy provides an improved methodology for synthesizing HEA-NPs on various supports.
Identification of therapeutics that target eEF1A2 and upregulate utrophin A translation in dystrophic muscles
Authors: Péladeau Christine , Adam Nadine , Bronicki Lucas M. , Coriati Adèle , Thabet Mohamed , Al-Rewashdy Hasanen , Vanstone Jason , Mears Alan , Renaud Jean-Marc , Holcik Martin , Jasmin Bernard J. 
Published: April 2020

AbstractUp-regulation of utrophin in muscles represents a promising therapeutic strategy for the treatment of Duchenne Muscular Dystrophy. We previously demonstrated that eEF1A2 associates with the 5’UTR of utrophin A to promote IRES-dependent translation. Here, we examine whether eEF1A2 directly regulates utrophin A expression and identify via an ELISA-based high-throughput screen, FDA-approved drugs that upregulate both eEF1A2 and utrophin A. Our results show that transient overexpression of eEF1A2 in mouse muscles causes an increase in IRES-mediated translation of utrophin A. Through the assessment of our screen, we reveal 7 classes of FDA-approved drugs that increase eEF1A2 and utrophin A protein levels. Treatment of mdx mice with the 2 top leads results in multiple improvements of the dystrophic phenotype. Here, we report that IRES-mediated translation of utrophin A via eEF1A2 is a critical mechanism of regulating utrophin A expression and reveal the potential of repurposed drugs for treating DMD via this pathway.
BCL9/STAT3 regulation of transcriptional enhancer networks promote DCIS progression
Authors: Elsarraj Hanan S. , Hong Yan , Limback Darlene , Zhao Ruonan , Berger Jenna , Bishop Stephanie C. , Sabbagh Aria , Oppenheimer Linzi , Harper Haleigh E. , Tsimelzon Anna , Huang Shixia , Hilsenbeck Susan G. , Edwards Dean P. , Fontes Joseph , Fan Fang , Madan Rashna , Fangman Ben , Ellis Ashley , Tawfik Ossama , Persons Diane L. , Fields Timothy , Godwin Andrew K. , Hagan Christy R. , Swenson-Fields Katherine , Coarfa Cristian , Thompson Jeffrey , Behbod Fariba 
Published: April 2020

AbstractThe molecular processes by which some human ductal carcinoma in situ (DCIS) lesions advance to the more aggressive form, while others remain indolent, are largely unknown. Experiments utilizing a patient-derived (PDX) DCIS Mouse INtraDuctal (MIND) animal model combined with ChIP-exo and RNA sequencing revealed that the formation of protein complexes between B Cell Lymphoma-9 (BCL9), phosphoserine 727 STAT3 (PS-727-STAT3) and non-STAT3 transcription factors on chromatin enhancers lead to subsequent transcription of key drivers of DCIS malignancy. Downregulation of two such targets, integrin β3 and its associated metalloproteinase, MMP16, resulted in a significant inhibition of DCIS invasive progression. Finally, in vivo targeting of BCL9, using rosemary extract, resulted in significant inhibition of DCIS malignancy in both cell line and PDX DCIS MIND animal models. As such, our studies provide compelling evidence for future testing of rosemary extract as a chemopreventive agent in breast cancer.
Ab initio theory of the negatively charged boron vacancy qubit in hexagonal boron nitride
Authors: Ivády Viktor , Barcza Gergely , Thiering Gergő , Li Song , Hamdi Hanen , Chou Jyh-Pin , Legeza Örs , Gali Adam 
Published: April 2020

AbstractHighly correlated orbitals coupled with phonons in two-dimension are identified for paramagnetic and optically active boron vacancy in hexagonal boron nitride by first principles methods which are responsible for recently observed optically detected magnetic resonance signal. Here, we report ab initio analysis of the correlated electronic structure of this center by density matrix renormalization group and Kohn-Sham density functional theory methods. By establishing the nature of the bright and dark states as well as the position of the energy levels, we provide a complete description of the magneto-optical properties and corresponding radiative and non-radiative routes which are responsible for the optical spin polarization and spin dependent luminescence of the defect. Our findings pave the way toward advancing the identification and characterization of room temperature quantum bits in two-dimensional solids.
Genetic predispositions of Parkinson’s disease revealed in patient-derived brain cells
Authors: Tran Jenne , Anastacio Helena , Bardy Cedric 
Published: April 2020

AbstractParkinson’s disease (PD) is the second most prevalent neurological disorder and has been the focus of intense investigations to understand its etiology and progression, but it still lacks a cure. Modeling diseases of the central nervous system in vitro with human induced pluripotent stem cells (hiPSC) is still in its infancy but has the potential to expedite the discovery and validation of new treatments. Here, we discuss the interplay between genetic predispositions and midbrain neuronal impairments in people living with PD. We first summarize the prevalence of causal Parkinson’s genes and risk factors reported in 74 epidemiological and genomic studies. We then present a meta-analysis of 385 hiPSC-derived neuronal lines from 67 recent independent original research articles, which point towards specific impairments in neurons from Parkinson’s patients, within the context of genetic predispositions. Despite the heterogeneous nature of the disease, current iPSC models reveal converging molecular pathways underlying neurodegeneration in a range of familial and sporadic forms of Parkinson’s disease. Altogether, consolidating our understanding of robust cellular phenotypes across genetic cohorts of Parkinson’s patients may guide future personalized drug screens in preclinical research.
Functional arrays of human pluripotent stem cell-derived cardiac microtissues
Authors: Thavandiran Nimalan , Hale Christopher , Blit Patrick , Sandberg Mark L. , McElvain Michele E. , Gagliardi Mark , Sun Bo , Witty Alec , Graham George , Do Van T.H. , Bakooshli Mohsen Afshar , Le Hon , Ostblom Joel , McEwen Samuel , Chau Erik , Prowse Andrew , Fernandes Ian , Norman Andreea , Gilbert Penney M. , Keller Gordon , Tagari Philip , Xu Han , Radisic Milica , Zandstra Peter W. 
Published: April 2020

AbstractTo accelerate the cardiac drug discovery pipeline, we set out to develop a platform that would be capable of quantifying tissue-level functions such as contractile force and be amenable to standard multiwell-plate manipulations. We report a 96-well-based array of 3D human pluripotent stem cell (hPSC)-derived cardiac microtissues - termed Cardiac MicroRings (CaMiRi) - in custom 3D-print-molded multiwell plates capable of contractile force measurement. Within each well, two elastomeric microcantilevers are situated above a circumferential ramp. The wells are seeded with cell-laden collagen, which, in response to the gradual slope of the circumferential ramp, self-organizes around tip-gated microcantilevers to form contracting CaMiRi. The contractile force exerted by the CaMiRi is measured and calculated using the deflection of the cantilevers. Platform responses were robust and comparable across wells, and we used it to determine an optimal tissue formulation. We validated the contractile force response of CaMiRi using selected cardiotropic compounds with known effects. Additionally, we developed automated protocols for CaMiRi seeding, image acquisition, and analysis to enable the measurement of contractile force with increased throughput. The unique tissue fabrication properties of the platform, and the consequent effects on tissue function, were demonstrated upon adding hPSC-derived epicardial cells to the system. This platform represents an open-source contractile force screening system useful for drug screening and tissue engineering applications.
Abdominal surgery prior to chronic psychosocial stress promotes spleen cell (re)activity and glucocorticoid resistance
Authors: Foertsch Sandra , Langgartner Dominik , Reber Stefan O. 
Published: April 2020

AbstractThere is convincing evidence from different mouse models that chronic psychosocial stress promotes splenomegaly, basal and lipopolysaccharide (LPS)-induced in vitro splenocyte activation and insensitivity towards glucocorticoids (GC) in in vitro LPS-treated splenocytes. However, we just recently showed, employing the chronic subordinate colony housing (CSC) paradigm, that bite wounds received during stressor exposure drive these stress-induced spleen changes. As skin wounds induced by planned surgery or physical trauma are more adequately reflecting what chronically stressed humans are likely to experience, it was the objective of the present study to investigate whether abdominal surgery prior to stressor exposure also promotes respective stress-induced spleen effects in the absence of any bite wounds. In line with our hypothesis, abdominal surgery prior to CSC induced splenomegaly, increased in vitro cell viability under basal and LPS conditions as well as the delta response to LPS (LPS – basal), and promoted the inability of isolated splenocytes to respond with a decreased cell viability to increasing concentrations of corticosterone following LPS-stimulation in vitro. Together with previous data, these findings demonstrate that physical injury, either in form of received bite wounds during stressor exposure or in form of abdominal surgery prior to stressor exposure, promotes the development of splenic immune activation and GC resistance.
Abscisic Acid and Glycine Betaine Mediated Tolerance Mechanisms under Drought Stress and Recovery in Axonopus compressus: A New Insight
Authors: Nawaz Mohsin , Wang Zhiyong 
Published: April 2020

AbstractChanging climatic scenarios affect plant growth and consequences are more malicious in drought conditions. This study was performed for better understanding of tolerance mechanisms under prevailing drought stress and succeeding recovery in Axonopus compressus by exogenously applied abscisic acid (ABA) and glycine betaine (GB). Three A. compressus accessions (A-38, A-58 and A-59) were subjected to well-watered (100% field capacity) and drought (40% field capacity) conditions. Two weeks later, plants were recovered from drought by re-watering. Water (control), GB, ABA and their combination were foliar applied on plants under drought twice a week until recovery. Drought stress decreased photosynthetic pigments and increased reactive oxygen species, lipid peroxidation, osmolytes and antioxidants in all accessions of A. compressus. Nonetheless, exogenous ABA and GB alone or in combination improved drought tolerance in all accessions which was maintained even after recovery. Maximum decrease in hydrogen peroxide and malondialdehyde, and increase in soluble sugars, proteins, proline, phenolics and chlorophyll contents, and superoxide dismutase, catalase, peroxidase and ascorbate peroxidase activity was recorded when GB was applied alone under drought. Order of improvement in drought tolerance among accessions was A-58 > A-59 > A-38. In conclusion, improved drought tolerance mechanisms by ABA and GB in A. compressus were retained even after recovery.
Reduced methane-bearing fluids as a source for diamond
Authors: Matjuschkin Vladimir , Woodland Alan B. , Frost Daniel J. , Yaxley Gregory M. 
Published: April 2020

AbstractDiamond formation in the Earth has been extensively discussed in recent years on the basis of geochemical analysis of natural materials, high-pressure experimental studies, or theoretical aspects. Here, we demonstrate experimentally for the first time, the spontaneous crystallization of diamond from CH4-rich fluids at pressure, temperature and redox conditions approximating those of the deeper parts of the cratonic lithospheric mantle (5–7 GPa) without using diamond seed crystals or carbides. In these experiments the fluid phase is nearly pure methane, even though the oxygen fugacity was significantly above metal saturation. We propose several previously unidentified mechanisms that may promote diamond formation under such conditions and which may also have implications for the origin of sublithospheric diamonds. These include the hydroxylation of silicate minerals like olivine and pyroxene, H2 incorporation into these phases and the “etching” of graphite by H2 and CH4 and reprecipitation as diamond. This study also serves as a demonstration of our new high-pressure experimental technique for obtaining reduced fluids, which is not only relevant for diamond synthesis, but also for investigating the metasomatic origins of diamond in the upper mantle, which has further implications for the deep carbon cycle.
Pharmacologic targeting of renal ischemia-reperfusion injury using a normothermic machine perfusion platform
Authors: Hameed Ahmer M. , Lu David B. , Burns Heather , Byrne Nicole , Chew Yi Vee , Julovi Sohel , Ghimire Kedar , Zanjani Negar Talaei , P’ng Chow H. , Meijles Daniel , Dervish Suat , Matthews Ross , Miraziz Ray , O’Grady Greg , Yuen Lawrence , Pleass Henry C. , Rogers Natasha M. , Hawthorne Wayne J. 
Published: April 2020

AbstractNormothermic machine perfusion (NMP) is an emerging modality for kidney preservation prior to transplantation. NMP may allow directed pharmacomodulation of renal ischemia-reperfusion injury (IRI) without the need for systemic donor/recipient therapies. Three proven anti-IRI agents not in widespread clinical use, CD47-blocking antibody (αCD47Ab), soluble complement receptor 1 (sCR1), and recombinant thrombomodulin (rTM), were compared in a murine model of kidney IRI. The most effective agent was then utilized in a custom NMP circuit for the treatment of isolated porcine kidneys, ascertaining the impact of the drug on perfusion and IRI-related parameters. αCD47Ab conferred the greatest protection against IRI in mice after 24 hours. αCD47Ab was therefore chosen as the candidate agent for addition to the NMP circuit. CD47 receptor binding was demonstrated by immunofluorescence. Renal perfusion/flow improved with CD47 blockade, with a corresponding reduction in oxidative stress and histologic damage compared to untreated NMP kidneys. Tubular and glomerular functional parameters were not significantly impacted by αCD47Ab treatment during NMP. In a murine renal IRI model, αCD47Ab was confirmed as a superior anti-IRI agent compared to therapies targeting other pathways. NMP enabled effective, direct delivery of this drug to porcine kidneys, although further efficacy needs to be proven in the transplantation setting.
A Bio-Economic Crop Yield Response (BECYR) Model for Corn and Soybeans in Ontario, Canada for 1959–2013
Authors: Xu Qin , Fox Glenn , McKenney Daniel W. , Parkin Gary , Li Zhenyi 
Published: April 2020

AbstractThis paper presents estimates of the effects of changing climate on crop yields for grain corn and soybeans in Ontario, Canada, for 1959–2013. We were able to use a database that is more comprehensive with respect to explanatory variables than some previous efforts had available. Our model includes climate variables, prices, land quality, groundwater level, CO2 concentration, and a time trend. Our results indicate that trends in temperature and precipitation during our study period have not yet resulted in appreciable threats to crop yields in the region.
Circularity in fisheries data weakens real world prediction
Authors: Giron-Nava Alfredo , Munch Stephan B. , Johnson Andrew F. , Deyle Ethan , James Chase C. , Saberski Erik , Pao Gerald M. , Aburto-Oropeza Octavio , Sugihara George 
Published: April 2020

AbstractThe systematic substitution of direct observational data with synthesized data derived from models during the stock assessment process has emerged as a low-cost alternative to direct data collection efforts. What is not widely appreciated, however, is how the use of such synthesized data can overestimate predictive skill when forecasting recruitment is part of the assessment process. Using a global database of stock assessments, we show that Standard Fisheries Models (SFMs) can successfully predict synthesized data based on presumed stock-recruitment relationships, however, they are generally less skillful at predicting observational data that are either raw or minimally filtered (denoised without using explicit stock-recruitment models). Additionally, we find that an equation-free approach that does not presume a specific stock-recruitment relationship is better than SFMs at predicting synthesized data, and moreover it can also predict observational recruitment data very well. Thus, while synthesized datasets are cheaper in the short term, they carry costs that can limit their utility in predicting real world recruitment.
Novel Variations in Native Ethiopian Goat breeds PRNP Gene and Their Potential Effect on Prion Protein Stability
Authors: Teferedegn Eden Yitna , Yaman Yalçın , Ün Cemal 
Published: April 2020

AbstractScrapie is a lethal neurodegenerative disease of sheep and goats caused by the misfolding of the prion protein. Variants such as M142, D145, S146, H154, Q211, and K222 were experimentally found to increase resistance or extend scrapie incubation period in goats. We aimed to identify polymorphisms in the Afar and Arsi-Bale goat breeds of Ethiopia and computationally assess the effect of variants on prion protein stability. In the present study, four non-synonymous novel polymorphisms G67S, W68R, G69D, and R159H in the first octapeptide repeat and the highly conserved C-terminus globular domain of goat PrP were detected. The resistant genotype, S146, was detected in >50% of the present population. The current study population showed a genetic diversity in Ethiopian goat breeds. In the insilico analysis, the R68 variant was predicted to increase stability while S67, D69, and H159 decrease the stability of prion protein. The new variants in the octapeptide repeat motif were predicted to decrease amyloidogenicity but H159 increased the hotspot sequence amyloidogenic propensity. These novel variants could be the source of conformational flexibility that may trigger the gain or loss of function by prion protein. Further experimental study is required to depict the actual effects of variants on prion protein stability.
Bridging repair of the abdominal wall in a rat experimental model. Comparison between uncoated and polyethylene oxide-coated equine pericardium meshes
Authors: Pasculli Alessandro , Gurrado Angela , De Luca Giuseppe Massimiliano , Mele Antonietta , Marzullo Andrea , Mangone Annarosa , Cellamare Saverio , Ferraro Valentina , Maqoud Fatima , Caggiani Maria Cristina , Rana Francesco , Cavallaro Giuseppe , Prete Francesco Paolo , Tricarico Domenico , Altomare Cosimo Damiano , Testini Mario 
Published: April 2020

AbstractBiological meshes improve the outcome of incisional hernia repairs in infected fields but often lead to recurrence after bridging techniques. Sixty male Wistar rats undergoing the excision of an abdominal wall portion and bridging mesh repair were randomised in two groups: Group A (N = 30) using the uncoated equine pericardium mesh; Group B (N = 30) using the polyethylene oxide (PEO)-coated one. No deaths were observed during treatment. Shrinkage was significantly less common in A than in B (3% vs 53%, P < 0.001). Adhesions were the most common complication and resulted significantly higher after 90 days in B than in A (90% vs 30%, P < 0.01). Microscopic examination revealed significantly (P < 0.05) higher mesh integrity, fibrosis and calcification in B compared to A. The enzymatic degradation, as assessed with Raman spectroscopy and enzyme stability test, affected A more than B. The PEO-coated equine pericardium mesh showed higher resistance to biodegradation compared to the uncoated one. Understanding the changes of these prostheses in a surgical setting may help to optimize the PEO-coating in designing new biomaterials for the bridging repair of the abdominal wall.