bluuebunny/crossref_metadata_2025_split huggingface dataset:
Opportunities for Prevention: A Data-Linkage Study to Inform A Public Health Response to Youth Offending in The Northern Territory, Australia
Authors: He Vincent Yaofeng , Leckning Bernard , Malvaso Catia , Williams Tamika , Liddle Leanne , Guthridge Steven 
Published: October 2020

Abstract
Background Numerous studies have demonstrated a strong link between child maltreatment and subsequent youth offending, leading to calls for early intervention initiatives. However, there have been few whole-population studies into the dimensions of statutory child maltreatment responses that can inform these programs. The aim of this study was to investigate the sex-specific association between level and timing of child protection system (CPS) contact and youth offending.Methods This retrospective cohort study used linked individual-level records from multiple agencies, for 10,438 Aboriginal children born in the Northern Territory between 1999 and 2006. The outcome measure was the first alleged offence. Key explanatory variables were level (no contact through to out-of-home care) and timing (0-4 years, 5-9 years, or both) of CPS contact. The Kaplan–Meier method was used to estimate cumulative incidence and a flexible parametric survival model to estimate hazard ratios (HR).Results Children with no record of CPS contact before age 10 had the lowest cumulative incidence of first offence by age 18 (boys: 23.4% [95%CI:21.0-26.1]; girls: 6.6% [95%CI:5.3-8.2]) and those with a record of out-of-home care the highest CI (boys: 45.5% [95%CI:37.0-54.9]; girls: 18.6% [95%CI:13.0-26.2]). The impact of CPS contact on risk of first alleged offence was greatest for children aged 10-13 years and decreased with age. Timing of CPS contact was also associated with increasing cumulative incidence. The relative risk for first offence was generally higher for children with CPS contact, of any type, during both developmental phases including notifications during both phases (boys, HR at age 11: 8.9 [95%CI:4.2-17.2]; girls, HR at age 11: 13.7 [95%CI:3.8-48.9]) and substantiations during both phases (boys, HR at age 11: 17.0 [95%CI:9.6-30.0]; girls, HR at age 11: 54.1 [95%CI:18.1-162]). Conclusion The increased risk of offending associated with level and timing of early CPS contact highlights opportunities for a differentiated public health response to improve life trajectories for children and to reduce youth crime. Although children with unsubstantiated notifications of maltreatment do not meet the criteria for a statutory CPS response, the higher risk of offending among these children supports their inclusion in targeted preventive interventions.
Translation and Cultural Adaptation of a Romanian Version of the Communication Assessment Tool (CAT_Ro)
Authors: Balanescu Andra Rodica , Bojinca Violeta Claudia , Schweitzer Ana Maria , Joca Bogdan , Mardale Denise Ani , Badea Denisa , Bojinca Mihai 
Published: October 2020

Abstract
Background: The communication between health providers and patients influences the quality of medical care. The Communication Skills Assessment (CAT) is a reliable, validated tool, which was developed to assess interpersonal communication skills between physicians and patients. The purpose of this study was to obtain a Romanian version of the CAT (CAT_Ro), using a controlled and systematic process to translate and cross-culturally adapt the original questionnaire, since there are no validated instruments to assess healthcare professionals’ communication capability in Romania. Methods: The study was conducted in two Departments of Internal Medicine and Rheumatology from Bucharest, Romania, using a rigorous scientific methodology for the translation process, according to literature recommendations, implicating conceptual evaluation, semantics, and cultural adaptation, which involved several steps. The updated version was pre-tested in a pilot study, which included 89 outpatients. Results: The results showed a narrow range of variability in item interpretation, without differences in patients’ responses according to variables such as age, gender, education, disease type, number of previous visits with the same doctor. Conclusion: CAT-Ro is the result of a comprehensive process study. It represents the first translation and cultural adaptation in Romanian of an instrument able to assess the health providers’ communication skills, which was validated in a pilot study and is to be used in more extensive studies with patients from several specialties.
Analysis of Clinical Characteristics, Laboratory Findings and Therapy of 134 Cases of COVID-19 in Wuhan, China: A Retrospective Analysis
Authors: Zhang Rui , Zhang Jie , Chen Jiebing 
Published: October 2020

Abstract
BackgroundAs everyone knows, the pandemic COVID-19 is spreading in the whole world. The number of laboratory-confirmed cases reached 28,637,211 and that of the death cases was 917,404 in the world as of September 13 th , 2020. We sought to analyse the clinical characteristics, laboratory findings and therapy of some cases with COVID-19.MethodsIn this retrospective study, we extracted the data on 134 patients with laboratory-confirmed COVID-19 in Wuhan Xinzhou District People's Hospital from January 16 th to April 24 th , 2020. Cases were confirmed by real-time RT-PCR and abnormal radiologic findings. Outcomes were followed up until May 1 th , 2020.ResultsCo-infection and severe underlying diseases made it easier for a case with COVID-19 to develop to be a severe one or reach an outcome of death. Age above 60 years old, male and symptoms such as fever, cough, chest tightness, headaches and fatigue were related to severe COVID-19 and an outcome of death. In addition, higher temperature, blood leukocyte count, neutrophil count, C-reactive protein level, D-dimer level, alanine aminotransferase activity, aspartate aminotransferase activity, α -hydroxybutyrate dehydrogenase activity, lactate dehydrogenase activity and creatine kinase activity were also related to severe COVID-19 and an outcome of death, and so was lower lymphocyte count. Administration of gamma globulin seemed helpful for reducing the mortality of patients with severe COVID-19, however the P value was greater than 0.05 (P=0.180), which mean under the same condition, studies of larger samples are needed in the future.ConclusionMultiple factors were related to severe COVID-19 and an outcome of death. Administration of gamma globulin seemed helpful for reducing the mortality of severe cases. More related studies are needed in the future.
A Tail of Two Structures: Proteomic and Transcriptomic Analysis of Cercarial Heads and Tails of Schistosome Worms
Authors: Hagerty James , Kim Hyung Chul , Jolly Emmitt 
Published: October 2020

Abstract
Schistosomes require both molluscan and mammalian hosts for development. The larval stage, cercaria, exits the intermediate snail host and must swim to identify and invade the definitive mammalian host. The cercaria has two macrostructures, the head and the tail. The tail is lost after host invasion, whereas the head develops into a sexually dimorphic adult worm. Translation in the cercaria differs in each macrostructure with higher levels of translation in cercarial tails and little to no translational activity in cercarial heads. We compared the transcriptome and proteome of the cercarial head and tail and observed stark differences between the two macrostructures. We identified unique and differentially expressed transcripts and proteins, including multiple ribosomal components expressed in higher levels in tails than in heads, which may explain the differences in translation levels between heads and tails and reflect the weak correlation between transcription and translation in infectious cercarial heads and tails.
Aetiology and Outcome of Non-Traumatic Coma in African Children: Protocol for a Systematic Review and Meta-Analysis
Authors: Ray Stephen , Fuller Charlotte , Boubour Alexandra , Bonnett Laura , Seydel Karl , Griffiths Michael 
Published: October 2020

Abstract
Background: Non-traumatic coma is a common acute childhood presentation to healthcare facilities in Africa and is associated with high morbidity and mortality. Historically, the majority of cases were attributed to cerebral malaria (CM). With the recent drastic reduction in malaria incidence, non-malarial coma is becoming a larger proportion of cases and determining the aetiology is diagnostically challenging, particularly in resource-limited settings. The purpose of this study will be to evaluate the aetiology and prognosis of non-traumatic coma in African children.Methods: From inception onwards, systematic searches of MEDLINE, Embase, and Scopus, will identify prospective and retrospective studies (including randomised controlled trials, cluster randomised trials, cohort studies, cross-sectional, and case-control studies) recruiting children (1 month-16 years) with non-traumatic coma (defined by Blantyre Coma Score ≤2 or comparable alternative) from any African country. Disease-specific studies will be included given that coma is associated and reported. The primary outcome is to determine the aetiology (infectious and non-infectious) of non-traumatic coma in African children, with pooled prevalence estimates of causes (e.g. malaria). Secondary outcomes are to determine overall estimates of morbidity and mortality of all cause non-traumatic coma and disease-specific states of non-traumatic coma, where available. Random effects meta-analysis will summarize aetiology data and in-hospital and post-discharge mortality. Heterogeneity will be quantified with τ2, I2, and Cochran’s Q test.Discussion: This systematic review will provide a summary of the best available evidence on the aetiology and outcome of non-traumatic coma in African children. Systematic Review Registration: This review is registered in PROSPERO International Prospective Register of Systematic Reviews (CRD42020141937).
Topological dislocations for plasmonic mode localization
Authors: Epstein Eliav , Singh Leeju , Fox Maayan , Sternklar Shmuel , Gorodetski Yuri 
Published: October 2020

Abstract
We study the localization of plasmonic modes on topological dislocations obtained by an abrupt change in the geometry of unit-cells in a plasmonic metasurface. We experimentally demonstrate mode localization in line defects and point singularities in the topology. These results are confirmed by numerical simulations of the near field distributions along the topology boundaries. We present structures with line dislocations supporting dark and bright modes. Moreover, we show that in structures with point dislocations the localization strength can be further manipulated by modifying the topological order of the structure.
PPM1D is Associated With Prognosis of Bladder Cancer in the Chinese Population
Authors: Cao Shuangqing , Zheng Lei 
Published: October 2020

Abstract
Background: Present study was to investigate the relative expression and prognostic performance of protein phosphatase magnesium/manganese-dependent 1D (PPM1D) in bladder cancer.Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) assay was performed to examine the relative expression of PPM1D mRNA in bladder cancer tissues and adjacent normal bladder tissues. The associations of PPM1D mRNA expression with clinicopathological features and the prognostic value were statistically analyzed via Chi-square test, Kaplan-Meier method and Cox regression analysis.Results: In comparison to adjacent normal tissues, PPM1D mRNA expression was obviously increased in bladder cancer tissues (P<0.001). Abnormal PPM1D expression was remarkably related to histological grade (P=0.017), TNM stage (P=0.032) and lymph nodes metastasis (P=0.035). Kaplan-Meier method showed that a close relationship was found between PPM1D expression and overall survival time (P=0.000). Multivariate analysis indicated that PPM1D expression (P=0.000, HR=3.530, 95%CI: 2.001-6.228) was a promising independent predictor for the prognosis of bladder cancer patients, as well as TNM stage (P=0.042, HR=1.768, 95%CI: 1.021-3.062).Conclusion: Taken together, our data showed that the potential performance of PPM1D as a prognostic biomarker and therapeutic target of bladder cancer.
What is the Impact of Infrapopliteal Endovascular Intervention on Free Flap Survival in Diabetic Foot Reconstruction?
Authors: Thai Duy Quang , Lee Dong Hwan , Lee Woo Beom , Hahn Hyung Min , Lee Il Jae 
Published: October 2020

Abstract
Background The combination of endovascular intervention and microvascular free flap transfer has been effectively used for the chronic ischemic wounds of lower limb. This study aimed to indicate the influence of angioplasty on the free flap survival in diabetic foot ulcer reconstruction.Methods A retrospective research was conducted of 46 diabetic patients with chronic ulcer of the foot. All the patients underwent the free flap reconstruction because of their non-healing wound with tendon or bone exposure. Patient’s demography, clinical data related to vascular status, vascular intervention, free flap transfer procedure were collected. Flap survival rate was compared between the group of severe arterial stenosis and non-severe group and between the different revascularization result groups as well. Results The average was 56.2 ± 10.8 years with 14 men (30.4%) and 32 women (69.6%). All of 23 patients (50%) who had severe infrapopliteal arterial stenosis underwent endovascular intervention. The final result of the pedal arch was type 1 in 13/23 patients (56.5%), type 2A and 2B in 7/23 and 2/23 patients respectively and type 3 in only one patient. Total flap necrosis was in 5 cases (10.9%), marginal necrosis in 4 cases (8.7%) and wound dehiscence in 4 cases (8.7%). There was no significant difference of flap loss between the severe arterial stenosis patients and non-severe arterial stenosis patients. In severe arterial stenosis group, after endovascular intervention, patients with type 1 of pedal arch had a significantly lower rate of total flap necrosis compared to the others. There was also no association between using of revascularized recipient artery and flap survival.Conclusions Our study revealed the crucial of quality of pedal arch on free flap survival. The PTA should aim to re-establish the complete pedal arch to increase the rate of wound healing as well as the flap success.
Somatic mutations in single human cardiomyocytes demonstrate accelerated age-related DNA damage and cell fusion
Authors: Walsh Christopher , Choudhury Sangita , Huang August , Kim Junho Junho , Morillo Katherine , Maury Eduardo , Zhou Zinan Zinan , Kenny Connor , Lee Eunjung , Chen Ming 
Published: October 2020

Abstract
The accumulation of somatic DNA mutations is a hallmark of aging in many dividing cells and contributes to carcinogenesis. Here we survey the landscape of somatic single-nucleotide variants (sSNVs) in heart muscle cells (cardiomyocytes) which normally do not proliferate but often become polyploid with age. Using single-cell whole-genome sequencing we analyzed sSNVs from 48 single cardiomyocytes from 10 healthy individuals (ages 0.4 - 82 yrs.). Cardiomyocyte sSNVs increased strikingly with age, at rates faster than reported in many dividing cells, or in non-dividing neurons. Analysis of nucleotide substitution patterns revealed age-related “clock-like” mutational signatures resembling those previously described. However, cardiomyocytes showed distinct mutational signatures that are rare or absent in other cells, implicating failed nucleotide excision repair of oxidative damage and defective mismatch repair (MMR) during aging. A lineage tree of cardiomyocytes, constructed using clonal sSNVs, revealed that some tetraploid (10%) and most cardiomyocytes with higher ploidy (>60%) derive from distinct clonal origins, implicating cell fusion as a mechanism contributing to many polyploid cardiomyocytes. Since age-accumulated sSNVs create dozens of damaging exonic mutations, cell fusion to form multiploid cardiomyocytes may represent an evolutionary mechanism of cellular genetic compensation that minimizes complete knockout of essential genes during aging. The rates and patterns of accumulation of cardiac mutations provide a paradigm to understand the influence of genomic aging on age-related heart disease.
Aberrant Wnt signalling induces comedo-like changes in the upper hair follicle
Authors: Shang Wei , Tan Alvin , Steensel Maurice , Lim Xinhong 
Published: October 2020

Abstract
Comedonal acne is a common skin disease characterized by cystic dilation of the hair follicle junctional zone. While the molecular mechanisms of acne pathogenesis are not well understood, an emerging hypothesis holds that imbalances in key signalling pathways may lead to abnormal fate determination of sebaceous progenitor cells. The subsequent accumulation of cells could then cause cyst formation in the hair follicle junctional zone and lower infundibulum, forming blackheads. This notion is supported by the observation that 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) causes severe comedonic acne and seboatrophy by inducing fate changes in Lrig1-expressing stem cells, as well as recent human GWAS studies implicating the Wnt signalling pathway in acne. To test the possible involvement of Wnt signals in blackhead formation, we used an Lrig1-CreERT2 mouse line to modulate Wnt signalling in stem cells of the junctional zone, which are known to contribute to the junctional zone, infundibulum, and sebaceous glands. We find that persistent activation of Wnt signalling in the junctional zone leads to formation of comedone-like cysts with associated atrophic sebaceous glands. The cysts strongly express stem cell markers and can be partially reduced by treatment with all-trans retinoic acid, a well-established acne treatment, as well as by inhibition of Hedgehog signalling. In contrast, loss of Wnt signalling leads to enlargement of the junctional zone, infundibulum and sebaceous glands. These data lead us to suggest that abnormal Wnt signalling could be involved in acne pathogenesis by inhibiting differentiation and retaining junctional zone cells in a proliferative progenitor state, leading to their accumulation into comedones.
Variable expressed methylation sites of aging-related genes in asthma
Authors: Yang Yu , Yuan Lin , Yang Ming , Du Xizi , Qin Ling , Wang Leyuan , Zhou Kai , Wu Mengping , He Ruoxi , Feng Juntao , Xiang Yang , Qu Xiangping , Liu Huijun , Qin Xiaoqun , Liu Chi 
Published: October 2020

Abstract
Background: Asthma is a complex pulmonary inflammatory disease which is common in the elderly. Aging-related alterations have also been found in the structural cells and immune cells of asthma patients although the pathological mechanism of the differential aging-related gene in the development of asthma is still obscure. Of note, DNA methylation (DNAm) have been proven to play an important role in the regulation of aging-related genes. However, the methylation levels of aging-related genes in asthma patients are largely unclear.Methods: First, the mRNA levels and DNAm level of the previous screened 9 aging-related genes in peripheral blood of 51 healthy controls (HCs) and 55 asthmatic patients were detected by multiple targeted bisulfite enrichment sequencing (MethTarget) and qPCR. Secondly, the correlation between the DNAm level of specific altered CpG sites and the pulmonary function indicators of asthma patients was evaluated. Lastly, the Receiver Operator Characteristic (ROC) curve and Principal Component Analysis (PCA) were used to identify the feasibility of the candidate CpG sites as asthma markers.Results: The mRNA expression of the 9 aging-related gene in peripheral blood of asthma patients was significantly different from those of HCs. Besides, the methylation level of the 9 aging-related genes also altered in asthma patients, and a total of 68 CpG sites were related to the severity of asthma. Notably, 10 of the 68 CpG sites had a significant relationship with pulmonary function parameters. Moreover, ROC curve and PCA analysis showed that the candidate differential methylation sites (DMSs) can be used as potential biomarkers for asthma.Conclusions: In summary, this study confirmed the changes in the mRNA expression and DNAm level of aging-related genes in asthma patients. The differential DMSs are associated with the clinical evaluation indicators of asthma, which may indicate the involvement of aging-related genes in the pathogenesis of asthma and provide some new possible biomarker of asthma.
Landscape of Lipidomic Metabolites in Gut-liver Axis of Sprague-Dawley Rats after Oral Exposure to Titanium Dioxide Nanoparticles
Authors: Chen Zhangjian , Han Shuo , Zheng Pai , Zhang Jiahe , Zhou Shupei , Jia Guang 
Published: October 2020

Abstract
Background: The application of titanium dioxide nanoparticles (TiO2 NPs) as food additives poses a risk of oral exposure that may lead to adverse health effects. Even though the substantial evidence supported liver as the target organ of TiO2 NPs via oral exposure, the mechanism of liver toxicity remains largely unknown. Since the liver is a key organ for lipid metabolism, this study focused on the landscape of lipidomic metabolites in gut-liver axis of Sprague Dawley (SD) rats exposed to TiO2 NPs at 0, 2, 10, 50 mg/kg body weight per day for 90 days.Results: TiO2 NPs (50 mg/kg) caused slight hepatotoxicity and changed lipidomic signatures of main organs or systems in the gut-liver axis including liver, serum and gut. The cluster profile from the above biological samples all pointed to the same key metabolic pathway and metabolites, which was glycerophospholipid metabolism and Phosphatidylcholines (PCs), respectively. In addition, absolute quantitative lipidomics verified the changes of three PCs concentrations, including PC(16:0/20:1), PC(18:0/18:0) and PC(18:2/20:2) in the serum samples after treatment of TiO2 NPs (50 mg/kg). The contents of malondialdehyde (MDA) in serum and liver increased significantly, which were positively correlated with most differential lipophilic metabolites.Conclusions: The gut was presumed to be the original site of oxidative stress and disorder of lipid metabolism, which resulted in hepatotoxicity through the gut-liver axis. Lipid peroxidation may be the initial step of lipid metabolism disorder induced by TiO2 NPs. Most nanomaterials (NMs) have oxidation induction and antibacterial properties, so the toxic pathway revealed in the present study may be primary and universal.