bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bedtime snacking deteriorates continuous glucose monitoring metrics in young children with type 1 diabetes: a randomized controlled crossover trial
Authors: Gökçe Tuğba , Karakuş Kağan , Mutlu Gül Yeşiltepe , Muradoğlu Serra , Eviz Elif , Can Ecem , Smart Carmel E , Hatun Şükrü , Özel Hülya Gökmen 
Published: December 2024

Abstract

Objective
To determine if a bedtime snack in young children with type 1 diabetes (T1D) prevents nocturnal hypoglycemia, and the impact on glycemia overnight.
Methods
In this randomized controlled crossover trial, 10 grams of carbohydrate (milk, yogurt, and kefir) was given 150–180 minutes after dinner over three nights to 5-8-year-old children with T1D using multiple daily injection therapy. Continuous glucose monitoring (CGM) data were collected for 6 hours following the snacks on one control and three snack nights. Time in 70–180 mg/dl range (TIR), time below 70 mg/dL (TBR), and other metrics were analyzed according to international CGM consensus. Trial day was terminated if blood glucose exceeded 300 mg/dL or fell below 70 mg/dL.
Results
Of 28 children (13 female, mean age 6.6 ± 0.8 years, HbA1c 7.0 ± 0.5%), mean glucose values before the test snacks were 137.8 ± 14.5 mg/dL for milk, 141.9 ± 16.9 mg/dL for yogurt, 136 ± 19.1 mg/dL for kefir, and 140.8 ± 17.0 mg/dL for control without significant difference (p = 0.548). TIR was 34.7% for milk, 38.7%. for yogurt, 45.9% for kefir, and 75.5% for control during the 6-hour post snack period, with TIR on the control day significantly higher than the three snack days (p < 0.001). TBR did not differ by group (p > 0.05). Of 112 trial days, 13 days were terminated due to hyperglycemia (> 300 mg/dL) (8 milk, 4 yogurt, 1 kefir), and 3 trial days due to hypoglycemia (< 70 mg/dL) (1 yogurt, 2 control).
Conclusion
Bedtime snacking in young children with T1D impairs nocturnal glycemia and reduces TIR, without decreasing the frequency of hypoglycemia.
Optimizing clinical nursing pathways to reduce the delay of thrombolysis in patients with acute ischemic stroke
Authors: Jiao Feitong , Wang Chunyan , Lu Jing , Zhou Pingping 
Published: December 2024

Abstract

Objective
To evaluate the effectiveness of optimizing clinical nursing pathways in reducing the delay of thrombolysis in patients with acute ischemic stroke.
Methodology: Process optimization implementation from July 2021 to December 2021 AIS patients who came to the emergency department of the Affiliated Hospital of Jilin Medical University (Jilin China) were included in the control group, referred to as T1. Process optimization from July 2022 to December 2022 The AIS patients who visited the clinic after implementation belonged to the T2 group. In June 2022, it will be the trial operation stage of optimizing the treatment process in the hospital, and will not be included in the study. The time from hospital admission to thrombolytic treatment and the situation of thrombolysis in the two groups were collected, including the time of physical assessment of the patients, CT time, the time of signing the thrombolysis agreement by the family members of the patients, and the time when the doctor was in place. Group T2 has stroke specialist nurses who participated in in-hospital training to guide the process and participate in the whole process of treatment.
Results
The median DNT in the T2 group was shortened from 39.5 minutes in the T1 group to 28.5 minutes, the proportion of DNT ≤ 45min and DNT ≤ 30min increased from 77.2–88.8%, and from 21.1–67.5%, the differences were statistically significant (all P < 0.01); the median on-site time of clinic doctors and thrombolytic doctors was shortened from 2.9 minutes to 2.6 minutes, and from 5.1 minutes to 4.3 minutes, respectively, with statistically significant differences (all P < 0.0001); process links The median time for the physical assessment of the middle-aged patients and the time from the completion of CT to the signing of the informed consent for intravenous thrombolysis were shortened from 15.2 minutes to 8.5 minutes, and from 11.6 minutes to 6.2 minutes (all P < 0.0001); transfer the patient to the CT and call the clinic The median time from the doctor to the completion of the physical examination and from the signing of the informed consent to the start of thrombolysis was shortened from 3.0 minutes to 2.6 minutes, 6.8 minutes to 6.7 minutes, and 4.3 minutes to 4.0 minutes respectively (all P < 0.05); There was no significant difference in NIHSS scores before treatment, 24 hours after treatment, and one week after treatment (P > 0.05). After 3 months of treatment, the proportion of mRS ≤ 2 points increased, but the differences in indicators were not statistically significant (P > 0.05).
Conclusion
The optimized clinical nursing path reduces the delay of thrombolysis in patients with acute ischemic stroke.
Influence of Sex and Gender on Knowledge of Generic Medicines Among Older Patients and Caregivers
Authors: Ceolin Chiara , Salis Francesco , Macaluso Myriam , Levati Elena , Zazzara Maria Beatrice , Onder Graziano , Bernabei Roberto , Trevisan Caterina , D'Ignazio Federica , Rivasi Gilia 
Published: December 2024

Abstract

Purpose
Generic medicines (GMs) are bioequivalent to brand-name drugs and more cost-effective, yet their use is limited. Gender-related factors may influence attitudes towards GMs, but data on older adults are scarce. This study aimed to explore sex and gender differences in knowledge and opinions about GMs among older patients and their caregivers.
Methods
SurFE is a cross-sectional multicenter survey conducted in Italy (April-May 2023) that enrolled non-institutionalized individuals aged ≥ 65 years and their caregivers. Participants completed a questionnaire assessing knowledge, attitudes, and perceptions of GMs, and sociodemographic and health-related information. We collected data on gender-specific factors and, using a validated methodology, developed a composite gender score.
Results
Among the 471 participants (312 patients and 159 caregivers), no differences related to biological sex were observed. According to the gender score, patients with male gender characteristics were less likely to believe that generic medicines are made with lower-quality substances. Additionally, both female patients and male caregivers were more likely to consider GMs equivalent to brand-name medications. Logistic regression analysis showed that female caregivers were significantly more likely to believe that GMs are produced with lower-quality substances (OR = 2.06, 95% CI 1.01–4.21, p = 0.047). No other significant associations between sex, gender, and beliefs about GMs were found.
Conclusions
Gender-related factors may have a greater impact on shaping beliefs about GMs than biological sex. Further research is required to explore the potential role of other sociodemographic or clinical factors to better understand and address barriers to the use of GMs.
Testing of a reusable chemical warming pad and an insulating jacket to manage hypothermia of preterm and low birthweight neonates
Authors: Ahmed Anisuddin , Rahman Fariya , Islam Md. Mahinur , Patwary Hridoy , Tanvir K M , Ahmed Saifuddin , Hussam Abul , Islam Mominul , Målqvist Mats , Rahman Ahmed Ehsanur , Arifeen Shams El , Rahman Syed Moshfiqur 
Published: December 2024

Abstract

Hypothermia remains a leading contributing factor to neonatal mortality. This study reports testing of a thermoregulatory device— ‘Thermal Jacket’ that includes a reusable chemical warming pad(CWP) and an insulating jacket designed for hypothermia management. The laboratory experiments were conducted in two distinct phases between February’21 and June’22. In phase 1, a ternary composite of Sodium-Acetate-Trihydrate, Glycerol, Paraffin, and water contained in a high-density polyethylene-pouch named ‘CWP’ was finalised, and an insulating jacket was designed for targeted heat retention. In phase 2, the device’s efficacy was evaluated using mannequin in a controlled setting. The sample size was 81 events. Welch's t-test, ANOVA, and GEE were used to assess any significant differences in performance with success. Among 81 events, approximately 93% of events of CWP and 98% of events of insulating jacket successfully maintained temperatures within 36–38°C for 120 minutes. Moreover, neither ambient temperature nor repeated use of CWPs had any significant effect on success rate of CWP and insulating jacket. Thermal Jacket had achieved and sustained the temperature range of 36–38°C for 2 hours. While this study used mannequin, rigorous systematic testing with preterm or low birthweight neonates in clinical settings is imperative to assess its effectiveness for thermal care management.
Increased Detection of Desulfobacterota in Wastewater Treatment Plant Effluent via Composite Sampling Versus Grab Sampling
Authors: Jurvansuu Jaana , Länsivaara Annika , Palmroth Marja , Kaarela Outi , Hyöty Heikki , Oikarinen Sami , Lehto Kirsi-Maarit 
Published: December 2024

Abstract

Municipal wastewater surveillance is essential for public health monitoring and preventing environmental contamination. Analysing treatment plant influents offers insights into population exposure to chemicals and microbes, while effluent and activated sludge reflect treatment efficiency and the quality of substances released into the environment. Our study, which compared grab and 24-hour composite bacterial sampling methods using next-generation sequencing of the 16S V4 region, offers practical insights. We collected samples from influent, activated sludge from the activated sludge process, and effluent wastewater on eight dates in 2017-2018 at wastewater treatment plants in Pirkanmaa, Finland. The results showed distinct bacterial compositions across sample types, with composite effluent samples revealing significantly higher Desulfobacterota abundance than the grab sample, thereby highlighting its higher resolution for effluent sampling.
Clinical outcomes of hepaticojejunostomy and ERCP endoscopic bile duct stent implantation in the treatment of malignant obstructive jaundice: a retrospective analysis
Authors: Liu Zhipeng , Han Ziqiang , Niu Zheyu , Hao Yijie , Yu Yuanzi , Zhu Huaqiang , Zhou Xu , Gao Hengjun , Lu Jun , Yang Faji 
Published: December 2024

Abstract

Purpose
Malignant obstructive jaundice (MOJ) refers to intrahepatic or extrahepatic bile ducts obstruction caused by the compression or infiltrative growth of malignant tumors, followed by cholestasis and jaundice. Hepaticojejunostomy (HJ) and Endoscopic Retrograde Cholangiopancreatography (ERCP) bile duct stent implantation are the common palliative treatment to reduce jaundice. The aim of current research was to investigate which treatment was better for patients with MOJ.
Patients and methods:
A retrospective analysis of 171 patients with distal bile duct obstruction due to malignancy admitted to the Department of Hepatobiliary Surgery and Gastroenterology in our hospital from February 2013 to August 2021. According to different treatment options, patients were divided into ERCP group (n = 64) and HJ group (n = 107). The primary end point was overall survival (OS); the secondary end points were postoperative bilirubin level, hospitalization time, recurrence time of obstructive jaundice, and the occurrence of recent postoperative complications.
Results
In this study, we found that There was no significant difference in the median OS between the ERCP group and the HJ group [mOS: 11.6 (95%CI9.4-13.8) months vs 13.3 (95%CI10.7-15.9) months, P = 0.978]. There was no statistical difference in the effect of reducing jaundice between the two groups (P > 0.05). The average hospitalization time in the ERCP group was 6.67 ± 4.02 days, which was significantly shorter than that in the HJ group (9.56 ± 5.38 days, P < 0.001). There was no significant difference in the total incidence of postoperative complications in the ERCP group compared with the HJ group (21.9% vs 21.5%, P = 0.953). However, pancreatitis often occurred in the ERCP group (P = 0.036). In terms of complications of biliary fistula, it mainly occurred in the HJ group (P = 0.029). Compared with the ERCP group, the recurrence time of obstructive jaundice was longer in the HJ group (P = 0.007).
Conclusion
This study showed that both treatments can effectively reduce bilirubin and improve liver function in patients with distal bile duct obstruction due to malignancy. Considering the long-term effect, HJ is superior to ERCP bile duct stenting.
Evaluation of the treatment response of postoperative anti-thyroglobulin antibody in patients with papillary thyroid cancer after 131I therapy
Authors: Hou Shasha , Tan Jian , Meng Zhaowei , Zhang Guizhi , Wang Peng , He Yajing , Zhang Fuhai , Zheng Wei , Wang Renfei 
Published: December 2024

Abstract

Background: Antithyroglobulin antibody (TgAb) affects the accuracy of thyroglobulin (Tg) detection in papillary thyroid cancer (PTC) patients after 131I therapy. This study aimed to evaluate TgAb trends after 131I therapy and investigate their prognostic value for predicting efficacy.
Methods: This retrospective study included 980 PTC patients who underwent total thyroidectomy and 131I treatment. TgAb levels were measured before and after 131I remnant ablation and 131I therapy. The success rates of 131I remnant ablation, time for TgAb to turn negative, and response to 131I therapy were analyzed based on TgAb trends. The effect and predictive value of TgAb levels and change amplitude (△TgAb) in PTC patients on 131I therapeutic efficacy (CR,PR, and NR) were evaluated.
Results: TgAb-positive patients had lower 131I remnant ablation success rates compared to TgAb-negative patients (55% vs. 75%, P=0.007). After the last 131I remnant ablation, 72.1% of patients turned TgAb-negative within 6 months. Higher initial TgAb levels, presence of Hashimoto's thyroiditis, and longer interval between surgery and 131I ablation were adverse indicators for TgAb turning negative. Patients with TgAb <226 IU/ml before 131I ablation and an interval <62 days between surgery and ablation were more likely to turn TgAb-negative within 6 months. The amplitude of TgAb variation was significantly higher in the effective 131I therapy group compared to the ineffective group (P<0.001).The optimal cutoffs for initial TgAb levels and ΔTgAb to predict 131I therapy efficacy were 473 IU/mL and 62%.
Conclusions: TgAb trends are closely related to the efficacy of 131I therapy in PTC patients. Patients with TgAb <226 IU/ml who achieved a negative TgAb status within six months following the initial 131I remnant ablation exhibited a significantly higher success rate for the initial ablation. 83.6% of patients achieved a negative status within one year following successful 131I residual ablation. A lower initial TgAb levels and a shorter interval between surgery and 131I ablation favor TgAb turning negative. ΔTgAb monitoring can provide a basis for predicting the prognosis of PTC patients after 131I therapy.
Transcriptome-based screening in TARDBP/TDP-43 knock-in motor neurons identifies the NEDD8-activating enzyme inhibitor MLN4924
Authors: Lépine Sarah , Maussion Gilles , Schneider Alexandria , Nauleau-Javaudin Angela , Castellanos-Montiel María José , Ambriz Georgina Jiménez , Spiegelman Dan , Abdian Narges , Franco-Flores Anna Krystina , Haghi Ghazal , Gursu Lale , Chaineau Mathilde , Durcan Thomas 
Published: December 2024

Abstract

A growing body of knowledge implicates perturbed RNA homeostasis in amyotrophic lateral sclerosis (ALS), a neurodegenerative disease that currently has no cure and few available treatments. Dysregulation of the multifunctional RNA-binding protein TDP-43 is increasingly regarded as a convergent feature of this disease, evidenced at the neuropathological level by the detection of TDP-43 pathology in most patient tissues, and at the genetic level by the identification of disease-associated mutations in its coding gene TARDBP. To characterize the transcriptional landscape induced by TARDBP mutations, we performed whole-transcriptome profiling of motor neurons differentiated from two knock-in iPSC lines expressing the ALS-linked TDP-43 variants p.A382T or p.G348C. Our results show that the TARDBP mutations significantly altered the expression profiles of mRNAs and microRNAs of the 14q32 cluster in MNs. Using mutation-induced gene signatures and the Connectivity Map database, we identified compounds predicted to restore gene expression toward wild-type levels. Among top-scoring compounds selected for further investigation, the NEDD8-activating enzyme inhibitor MLN4924 effectively improved cell viability and neuronal activity, highlighting a possible role for protein post-translational modification via NEDDylation in the pathobiology of TDP-43 in ALS.
Nanodiamond-embedded tungsten disulfide nanosheets for electrochemical detection of a bacterial metabolite pyocyanin
Authors: Keerthana S. , Divya K. P. , Rajapriya A. , Viswanathan C. , Ponpandian N. 
Published: December 2024

Abstract

Pyocyanin (PYO) is a distinctive electroactive virulence factor secreted by Pseudomonas aeruginosa, a bacterium implicated in a spectrum of severe human infections, particularly in susceptible and critically ill patients. The rapid and accurate diagnosis of infectious agents is crucial for prescribing effective antibiotics and ensuring successful treatment outcomes in patients facing critical conditions. Due to its redox-active nature, PYO actively participates in electron acceptance and donation processes at the electrode surface, making it well-suited for electrochemical detection. To enhance this detection capability, we utilized a physically cross-linked methodology to embellish the surface of a working electrode with a nanocomposite composed of tungsten disulfide nanosheets supported by nanodiamond (WS2 NSs-ND). This innovative approach was implemented to enhance electron transport, and sample absorption on the electrode surface with a sensitivity of 0.38 µA µM− 1 cmdue to its increased number of active sites. Furthermore, WS2 NSs-ND demonstrated remarkable electrochemical performance when applied to real sample human urine, yielding RSD of ± 2%. This underscores the reliability and robustness of WS2 NSs-ND/GCE for the accurate detection of PYO in diverse biological samples, showcasing its potential for clinical applications.
Status epilepticus induced by etanercept biosimilar: a rare case report
Authors: Wang Yangai , Liu Gang 
Published: December 2024

Abstract

Objective
To emphasizes the likelihood of seizure adverse effects linked to rhTNFR-Fc biosimilar.
Methods
A 74-year-old male patient with rheumatoid arthritis experienced a status epilepticus induced by rhTNFR-Fc, which was diagnosed on the basis of brain magnetic resonance imaging and electroencephalography. Anti-TNF medications, including the etanercept biosimilar rhTNFR-Fc, have been linked to the occurrence of seizures. In the event of a seizure adverse event (AE), it is recommended that the suspected drug be discontinued and anti-epileptic drugs be administered to control the seizure.
Results
The administration of rhTNFR-Fc was terminated, and the seizure was managed through the maintenance of levetiracetam and valproic acid treatment.
Conclusion
The rhTNFR-Fc biosimilar may cause seizure adverse effects in patients without any prior history and signifies the critical importance of a neurological assessment and close clinical monitoring being performed in patients with pre-existing seizure disorders before and during therapy with TNF-α inhibitors.
Assembly of the complete mitochondrial genome of Prinsepia uniflora (Rosaceae): Insights into genomic characterization and phylogenetic analysis
Authors: Ma Lu , Chen Shengwei , Zhang Erdong , An Yajing , Zhang Lei 
Published: December 2024

Abstract

Background
Prinsepia uniflora is a significant medicinal and ecological value shrub. Although the genome of this species has been sequenced at the chromosome scale, reports on the mitogenomes of Prinsepia plants are lacking, substantially impedes further research on this species.
Results
We used PacBio HiFi sequencing to assemble the mitogenome of P. uniflora. The mitogenome was characterized is a circular chromosome, with a total length of 328,095 bp; the genes included 35 protein-coding genes, 18 transfer RNA (tRNA) genes and 3 ribosomal RNA (rRNA) genes. Further, our mitogenome characteristics investigation of the P. uniflora included codon usage, sequences repeats and selective pressure. The results showed that a large number of short repetitive sequences existed in the mitogenome of P. uniflora, the codon usage showed a preference for codons ending in A/T, and the codon use bias of the PCGs was modified by natural orartificial selection. We also identifed 20 large fragments that were transferred from the chloroplast genome to the mitogenome. The collinear analysis of mitochondrial structures between P. uniflora and Rosaceae species indicated that P. uniflora was closely related to Prunus padus. Phylogenetic examination based on ML and BI methods also revealed the evolutionary status of the P. uniflora.
Conclusion
In this study, we assembled and annotated the first mitogenome of the Prinsepia. The subsequent analysis provided us with a basis for further molecular studies on Prinsepia mitogenomes and valuable information on the evolutionary biology of Rosaceae species.
Clinical application of a simplified hysteroscopic LNG-IUD non-suture fixation in the treatment of adenomyosis
Authors: Yu Yi , Cong Qing , Sui Long , Zhang Hongwei , Chen Limei 
Published: December 2024

Abstract

Background
Placement of a levonorgestrel-releasing intrauterine device (LNG-IUD) is an effective treatment forpatients diagnosed with adenomyosis who had a strong desire to preserve the uterus and had no immediate fertility requirements. However, there are also some limitations to the suitability of the LNG-IUD for women with adenomyosis, as adenomyosis causes distortion or enlargement of the uterine cavity. Therefore, the LNG-IUD is more prone to expulsion in patients with adenomyosis. In this study, we aimed to show an original and simplified approach using the hysteroscope for non-suture ﬁxation of LNG-IUD in patients with adenomyosis.
Methods
In this retrospectivecase series approved by the Ethics Committee of Obstetrics and Gynecology Hospital of Fudan University, sixty-two patients diagnosed with adenomyosis who had a strong desire to preserve the uterus and had no immediate fertility requirements in the short term were included in the study. They underwent hysteroscopic non-suture fixation of LNG-IUD. All patients underwent one-year postoperative follow-up to evaluate the LNG-IUD expulsion rate, postoperative efficacy and side effects.
Results
Sixty-two patients with adenomyosis underwent non-suture fixation with LNG-IUD and during the long-term postoperative follow-up, every patient experienced complete remission of their symptoms: a significant decrease in menstrual flow, relief of dysmenorrhea, and improvement in quality of life. Only two patients experienced expulsion of the LNG-IUD at 6 months and 10 months postoperatively, respectively.
Conclusions
Hysteroscopic non-suture fixation of the LNG-IUD can be a more simplified and minimally invasive alternative treatment for patients with a history of LNG-IUD expulsion. The non-suture method greatly reduces the complexity of hysteroscopic fixation and can be performed more efficiently in office.