bluuebunny/crossref_metadata_2025_split huggingface dataset:
Brightness-gated two-color coincidence detection unravels two distinct mechanisms in bacterial protein translation initiation
Authors: Höfig Henning , Yukhnovets Olessya , Remes Cristina , Kempf Noemie , Katranidis Alexandros , Kempe Daryan , Fitter Jörg 
Published: December 2019

AbstractLife on the molecular scale is based on a complex interplay of biomolecules under which the ability of binding is crucial. Fluorescence based two-color coincidence detection (TCCD) is commonly used to characterize molecular binding, but suffers from an underestimation of coincident events. Here, we introduce a brightness-gated TCCD which overcomes this limitation and benchmark our approach with two custom-made calibration samples. Applied to a cell-free protein synthesis assay, brightness-gated TCCD unraveled a previously disregarded mode of translation initiation in bacteria.
20 years of developments in optical frequency comb technology and applications
Authors: Fortier Tara , Baumann Esther 
Published: December 2019

AbstractOptical frequency combs were developed nearly two decades ago to support the world’s most precise atomic clocks. Acting as precision optical synthesizers, frequency combs enable the precise transfer of phase and frequency information from a high-stability reference to hundreds of thousands of tones in the optical domain. This versatility, coupled with near-continuous spectroscopic coverage from microwave frequencies to the extreme ultra-violet, has enabled precision measurement capabilities in both fundamental and applied contexts. This review takes a tutorial approach to illustrate how 20 years of source development and technology has facilitated the journey of optical frequency combs from the lab into the field.
A functional polymorphism rs10830963 in melatonin receptor 1B associated with the risk of gestational diabetes mellitus
Authors: Huang Bo , Wang Yu-kun , Qin Lin-yuan , Wei Qin , Liu Nian , Jiang Min , Yu Hong-ping , Yu Xiang-yuan 
Published: December 2019

Abstract
The melatonin receptor 1B (MTNR1B) polymorphism rs10830963 C&gt;G has been reported to be associated with the risk of gestational diabetes mellitus (GDM) with inconsistent results. To clarify the effect of the polymorphism on the risk of GDM, a meta-analysis therefore was performed. Pooled OR with its corresponding 95%CI was used to estimate the strength of the association. Totally 14 eligible studies with a number of 5033 GDM patients and 5614 controls were included in this meta-analysis. Results indicated that the variant G allele was significantly associated with an increased GDM risk (CG vs. CC: OR = 1.25, 95% CI = 1.11−1.40, P &lt; 0.001; GG vs. CC: OR = 1.78, 95% CI = 1.45−2.19, P &lt; 0.001; G vs. C: OR = 1.33, 95% CI = 1.21−1.47, P &lt; 0.001). In the stratified analysis by ethnicity, similar results were found in Asians (CG vs. CC: OR = 1.15, 95%CI = 1.02−1.28, P = 0.020; GG vs. CC: OR = 1.52, 95% CI = 1.23−1.89, P &lt; 0.001; G vs. C: OR = 1.23, 95% CI = 1.10−1.37, P &lt; 0.001) and in Caucasians (CG vs. CC: OR = 1.40, 95% CI = 1.16−1.70, P &lt; 0.001; GG vs. CC: OR = 2.21, 95% CI = 1.54−3.17, P &lt; 0.001; G vs. C: OR = 1.47, 95% CI = 1.24−1.73, P &lt; 0.001). FPRP and TSA analyses confirmed findings support that the rs10830963 G allele increases the risk of GDM, and further functional experimental studies are warranted to explore and clarify the potential mechanism.
Integrative analysis of shared genetic pathogenesis by autism spectrum disorder and obsessive-compulsive disorder
Authors: Liu Dongbai , Cao Hongbao , Kural Kamil Can , Fang Qi , Zhang Fuquan 
Published: December 2019

Abstract
Many common pathological features have been observed for both autism spectrum disorders (ASDs) and obsessive-compulsive disorder (OCD). However, no systematic analysis of the common gene markers associated with both ASD and OCD has been conducted so far. Here, two batches of large-scale literature-based disease–gene relation data (updated in 2017 and 2019, respectively) and gene expression data were integrated to study the possible association between OCD and ASD at the genetic level. Genes linked to OCD and ASD present significant overlap (P-value &lt;2.64e-39). A genetic network of over 20 genes was constructed, through which OCD and ASD may exert influence on each other. The 2017-based analysis suggested six potential common risk genes for OCD and ASD (CDH2, ADCY8, APOE, TSPO, TOR1A, and OLIG2), and the 2019-based study identified two more genes (DISP1 and SETD1A). Notably, the gene APOE identified by the 2017-based analysis has been implicated to have an association with ASD in a recent study (2018) with DNA methylation analysis. Our results support the possible complex genetic associations between OCD and ASD. Genes linked to one disease are worth further investigation as potential risk factors for the other.
Dynamic characteristics of lipid metabolism in cultured granulosa cells from geese follicles at different developmental stages
Authors: Gao Shanyan , Gan Xiang , He Hua , Hu Shenqiang , Deng Yan , Chen Xi , Li Li , Hu Jiwei , Li Liang , Wang Jiwen 
Published: December 2019

Abstract
Previous studies have shown that lipid metabolism in granulosa cells (GCs) plays a vital role during mammalian ovarian follicular development. However, little research has been done on lipid metabolism in avian follicular GCs. The goal of the present study was to investigate the dynamic characteristics of lipid metabolism in GCs from geese pre-hierarchical (6–10 mm) and hierarchical (F4-F2 and F1) follicles during a 6-day period of in vitro culture. Oil red O staining showed that with the increasing incubation time, the amount of lipids accumulated in three cohorts of GCs increased gradually, reached the maxima after 96 h of culture, and then decreased. Moreover, the lipid content varied among these three cohorts, with the highest in F1 GCs. The qPCR results showed genes related to lipid synthesis and oxidation were highest expressed in pre-hierarchical GCs, while those related to lipid transport and deposition were highest expressed in hierarchical GCs. These results suggested that the amount of intracellular lipids in GCs increases with both the follicular diameter and culture time, which is accompanied by significant changes in expression of genes related to lipid metabolism. Therefore, it is postulated that the lipid accumulation capacity of geese GCs depends on the stage of follicle development and is finely regulated by the differential expression of genes related to lipid metabolism.
Development and validation of a nomogram to predict the prognosis of patients with squamous cell carcinoma of the bladder
Authors: Hu Mei-Di , Chen Si-Hai , Liu Yuan , Jia Ling-Hua 
Published: December 2019

Abstract
Background: The present study aimed to develop and validate a nomogram based on expanded TNM staging to predict the prognosis for patients with squamous cell carcinoma of the bladder (SCCB).
Methods: A total of 595 eligible patients with SCCB identified in the Surveillance, Epidemiology, and End Results (SEER) dataset were randomly divided into training set (n = 416) and validation set (n = 179). The likelihood ratio test was used to select potentially relevant factors for developing the nomogram. The performance of the nomogram was validated on the training and validation sets using a C-index with 95% confidence interval (95% CI) and calibration curve, and was further compared with TNM staging system.
Results: The nomogram included six factors: age, T stage, N stage, M stage, the method of surgery and tumor size. The C-indexes of the nomogram were 0.768 (0.741–0.795) and 0.717 (0.671–0.763) in the training and validation sets, respectively, which were higher than the TNM staging system with C-indexes of 0.580 (0.543–0.617) and 0.540 (0.484–0.596) in the training and validation sets, respectively. Furthermore, the decision curve analysis (DCA) proved that the nomogram provided superior clinical effectiveness.
Conclusions: We developed a nomogram that help predict individualized prognosis for patients with SCCB.
Mitochondrial dynamic modulation exerts cardiometabolic protection in obese insulin-resistant rats
Authors: Maneechote Chayodom , Palee Siripong , Apaijai Nattayaporn , Kerdphoo Sasiwan , Jaiwongkam Thidarat , Chattipakorn Siriporn C. , Chattipakorn Nipon 
Published: December 2019

Abstract
Obese insulin resistance impairs cardiac mitochondrial dynamics by increasing mitochondrial fission and decreasing mitochondrial fusion, leading to mitochondrial damage, myocardial cell death and cardiac dysfunction. Therefore, inhibiting fission and promoting fusion could provide cardioprotection in this pre-diabetic condition. We investigated the combined effects of the mitochondrial fission inhibitor (Mdivi1) and fusion promoter (M1) on cardiac function in obese insulin-resistant rats. We hypothesized that Mdivi1 and M1 protect heart against obese insulin-resistant condition, but also there will be greater improvement using Mdivi1 and M1 as a combined treatment. Wistar rats (n=56, male) were randomly assigned to a high-fat diet (HFD) and normal diet (ND) fed groups. After feeding with either ND or HFD for 12 weeks, rats in each dietary group were divided into groups to receive either the vehicle, Mdivi1 (1.2 mg/kg, i.p.), M1 (2 mg/kg, i.p.) or combined treatment for 14 days. The cardiac function, cardiac mitochondrial function, metabolic and biochemical parameters were monitored before and after the treatment. HFD rats developed obese insulin resistance which led to impaired dynamics balance and function of mitochondria, increased cardiac cell apoptosis and dysfunction. Although Mdivi1, M1 and combined treatment exerted similar cardiometabolic benefits in HFD rats, the combined therapy showed a greater reduction in mitochondrial reactive oxygen species (ROS). Mitochondrial fission inhibitor and fusion promoter exerted similar levels of cardioprotection in a pre-diabetic condition.
USco1621 B and USco1556 B: Two wide companions at the deuterium-burning mass limit in Upper Scorpius
Authors: Chinchilla Patricia , Béjar Víctor J. S. , Lodieu Nicolas , Gauza Bartosz , Zapatero Osorio Maria Rosa , Rebolo Rafael , Garrido Antonio Pérez , Alvarez Carlos , Manjavacas Elena 
Published: December 2019

Aims. Our objective is to identify analogues of gas giant planets, but located as companions at wide separations of very young stars. The main purpose is to characterise the binarity frequency and the properties of these substellar objects, and to elucidate their early evolutionary stages.
Methods. To identify these objects, we cross correlated the Visible and Infrared Survey Telescope for Astronomy Hemisphere Survey and the United Kingdom Infrared Telescope Infrared Deep Sky Survey Galactic Clusters Survey catalogues to search for common proper motion companions to 1195 already known members of Upper Scorpius (USco; age ~5–10 Myr, distance ~145 pc). We present the discovery and spectroscopic characterisation of two very wide substellar companions of two early-M stars in Upper Scorpius: USco1621 B and USco1556 B. We obtained optical and near-infrared low-resolution spectroscopy of the candidates to characterise their spectral energy distribution and confirm their youth and membership to the association. We also acquired adaptive optics images of the primaries and secondaries to search for signs of binarity and close companions.
Results. By comparison with field dwarfs and other young members of USco, we determined a spectral type of M8.5 in the optical for both companions, along with L0 and L0.5 in the near-infrared for USco1621 B and USco1556 B, respectively. The spectra of the two companions show evident markers of youth, such as weak alkaline Na I and K I lines, along with the triangular shape of the H-band. The comparison with theoretical evolutionary models gives estimated masses of 0.015 ± 0.002 and 0.014 ± 0.002 M⊙, with temperatures of 2270 ± 90 and 2240 ± 100 K, respectively. The physical separations between the components of both systems are 2880 ± 20 and 3500 ± 40 AU for USco1621 and USco1556 systems, respectively. We did not find any additional close companion in the adaptive optics images. The probability that the two secondaries are physically bound to their respective primaries, and not chance alignments of USco members, is 86%, and the probability that none of them are physically related is 1.0%.
The ALMA Frontier Fields Survey
Authors: Carvajal R. , Bauer F. E. , Bouwens R. J. , Oesch P. A. , González-López J. , Anguita T. , Aravena M. , Demarco R. , Guaita L. , Infante L. , Kim S. , Kneissl R. , Koekemoer A. M. , Messias H. , Treister E. , Villard E. , Zitrin A. , Troncoso P. 
Published: December 2019

Context.The Hubble Frontier Fields offer an exceptionally deep window into the high-redshift universe, covering a substantially larger area than the Hubble Ultra-Deep field at low magnification and probing 1–2 mag deeper in exceptional high-magnification regions. This unique parameter space, coupled with the exceptional multi-wavelength ancillary data, can facilitate for useful insights into distant galaxy populations.Aims.We aim to leverage Atacama Large Millimetre Array (ALMA) band 6 (≈263 GHz) mosaics in the central portions of five Frontier Fields to characterize the infrared (IR) properties of 1582 ultraviolet (UV)-selected Lyman-Break Galaxies (LBGs) at redshifts ofz ∼ 2–8. We investigated individual and stacked fluxes and IR excess (IRX) values of the LBG sample as functions of stellar mass (M⋆), redshift, UV luminosity and slopeβ, and lensing magnification.Methods.LBG samples were derived from color-selection and photometric redshift estimation withHubbleSpace Telescope photometry. Spectral energy distributions -templates were fit to obtain luminosities, stellar masses, and star formation rates for the LBG candidates. We obtained individual IR flux and IRX estimates, as well as stacked averages, using both ALMA images andu–vvisibilities.Results.Two (2) LBG candidates were individually detected above a significance of 4.1-σ, while stacked samples of the remaining LBG candidates yielded no significant detections. We investigated our detections and upper limits in the context of the IRX–M⋆and IRX–βrelations, probing at least one dex lower in stellar mass than past studies have done. Our upper limits exclude substantial portions of parameter space and they are sufficiently deep in a handful of cases to create mild tension with the typically assumed attenuation and consensus relations. We observe a clear and smooth trend betweenM⋆andβ, which extends to low masses and blue (low)βvalues, consistent with expectations from previous works.
Urethrolithiasis beim Hund – eine retrospektive Untersuchung an 83 Rüden
Authors: Thiel Cetina , Häußler Thomas Christian , Kramer Martin , Tacke Sabine 
Published: December 2019

Zusammenfassung
Gegenstand und Ziel Die Urethrolithiasis ist eine häufige Ursache für Harnabsatzstörungen. Ziel der retrospektiven
Studie war, Fälle von Rüden zu evaluieren, bei denen Harnröhrensteine unter standardisierter Epiduralanästhesie in die
Harnblase zurückverlagert wurden.
Material und Methode Die Daten von 83 männlichen Hunden mit Urethrolithiasis wurden hinsichtlich klinischer
Symptome, Lokalisation der Harnsteine, Anzahl der Urethrolithen, Art der bildgebenden Diagnostik und des operativen
Eingriffs sowie der Ergebnisse der postoperativen Röntgenkontrollen ausgewertet. Zusätzlich erfolgte eine Auswertung der
bakteriologischen Urinuntersuchungen und Steinanalysen. Alle Patienten erhielten zusätzlich zu einer Allgemeinanästhesie
eine standardisierte Epiduralanästhesie.
Ergebnisse Mit einer Ausnahme wurden alle der 83 Rüden wegen Harnabsatzbeschwerden vorgestellt, die bei 33
Patienten chronisch waren. In 66 Fällen ließen sich röntgendichte Konkremente in der Harnröhre nachweisen, 11-mal wurde
die Diagnose Urethrolithiasis mittels sonografischer Untersuchung gestellt und bei 6 Patienten führte eine
Katheterisierung mit nachfolgendem Nachweis der Harnsteine in der Harnblase zur Diagnose Urethrolithiasis. Mehr als ein
Drittel der Hunde wiesen zum Vorstellungszeitpunkt ausschließlich Konkremente in der Urethra auf. Bei 44 Rüden wurden 3
oder mehr Urethrasteine diagnostiziert. Bei 77 von 83 Hunden (92,7 %) gelang es, alle Harnsteine unter
Epiduralanästhesie in die Harnblase zurückzuverlagern. Neun Patienten mit röntgendichten Harnsteinen wiesen bei der
postoperativen radiologischen Kontrolle residuale Konkremente auf.
Schlussfolgerung Für die zuverlässige radiologische Diagnostik von Harnsteinen beim Rüden muss bedacht werden,
dass sich ein nicht unerheblicher Anteil ausschließlich in der Harnröhre befindet. Das Vorliegen röntgendurchlässiger
Urethrasteine kann durch sonografische Untersuchung der Harnröhre evaluiert werden, die alleinige
Ultraschalluntersuchung der Harnblase reicht hier nicht aus. Eine Epiduralanästhesie sollte spätestens dann in Erwägung
gezogen werden, wenn Spülversuche zur Behebung der urethralen Obstruktion erfolglos bleiben. Die postoperative
radiologische Kontrolle nach Laparozystotomie hinsichtlich einer vollständigen Steinentfernung ist bei röntgendichten
Urolithen auf jeden Fall anzuraten.
Neuigkeiten zur praktischen ABC-Blutgruppen-Bestimmung bei Katzen
Authors: Kehl Alexandra , Truchet Laura , Langbein-Detsch Ines , Müller Elisabeth , Giger Urs 
Published: December 2019

ZusammenfassungBis in die 1980er Jahre wurden die Blutgruppen bei Katzen als unwichtig angesehen. Seitdem gab es jedoch viele neue
Erkenntnisse. Wir wissen heute: Das wichtigste Blutgruppensystem der Katze ist das AB-Blutgruppensystem
(umbenannt als ABC-Blutgruppensystem) und umfasst die Blutgruppen A, B und AB (heute
C genannt). Katzen haben natürlich vorkommende Antikörper gegen die Antigene der jeweils anderen Blutgruppe.
Insbesondere Katzen mit Blutgruppe B haben hohe Titer an natürlich vorkommenden Anti-A-Alloantikörpern.
Dadurch kann es bei A-B-inkompatiblen Bluttransfusionen und bei der sog. neonatalen Isoerythrolyse bei Welpen mit
Typ A und C (AB) von Muttertieren mit Typ B zu Unverträglichkeitsreaktionen kommen. Um dies
zu verhindern, ist es essenziell, für Zucht und im Vorfeld von Transfusionen die Blutgruppe und die zugrundeliegende
Genetik zu kennen. Klinisch helfen serologische und genetische Tests. Diese Synopsis gibt einen aktuellen Überblick über
die Blutgruppen, deren physiologische und genetische Grundlagen, mögliche Unverträglichkeitsreaktionen sowie die
verschiedenen Nachweismethoden zu deren Vermeidung.
14.4.12 4H-1-Benzopyran-4-ones (Update 2019)
Authors: Dong X. , Liu H. 
Published: December 2019

AbstractThis chapter is an update to the earlier Science of Synthesis contribution (Section 14.4.3) describing methods for the synthesis of 4H-1-benzopyran-4-ones. It focuses on the literature published in the period 2004—2018, with most methods involving cyclization to form the oxygen-containing ring.
14.11.3 Selenopyranones and Benzoselenopyranones (Update 2019)
Authors: Chen C. , Yu L. 
Published: December 2019

AbstractThis chapter is an update to the earlier Science of Synthesis contribution (Section 14.11) describing methods for the synthesis of selenopyranones and benzoselenopyranones. Recent interest in this area has in part been generated by the discovery that some 9H-selenoxanthen-9-ones and selenopyran in particular, exhibit very good absorption of near-infrared light, which means they have potential applications in many fields such as medicine, sensors/detectors, and catalysis.
Increased stiffness and flow resistance of the inner wall of Schlemm’s canal in glaucomatous human eyes
Authors: Vahabikashi Amir , Gelman Ariel , Dong Biqin , Gong Lihua , Cha Elliott D. K. , Schimmel Margit , Tamm Ernst R. , Perkumas Kristin , Stamer W. Daniel , Sun Cheng , Zhang Hao F. , Gong Haiyan , Johnson Mark 
Published: December 2019

Significance
Glaucoma is a leading cause of irreversible blindness. The elevated intraocular pressure that is characteristic of primary open-angle glaucoma is due to increased resistance to aqueous humor outflow. Localizing this increased resistance has eluded investigators until recently. We report here that the glaucomatous flow resistance can be found within approximately 1 µm of the surface of the endothelial lining of Schlemm’s canal in the aqueous humor outflow pathway of the eye. There is a greatly increased tissue stiffness in this same narrow region. Exploring and targeting these highly localized biophysical changes will allow development of new therapies for glaucoma.
CRISPR-Cas technology in modifying food crops.
Authors: Tofazzal Islam Tofazzal Islam 
Published: December 2019

Abstract
The population in the world is expected to increase from 7.3 to 9.7 billion by 2050. Outbreak of pest and diseases and increased abiotic stresses due to global climate change poses a high risk to crop production to feed the rapidly increasing population. The adaptive immune system CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated) in prokaryotes has led to a revolution in targeted genome editing with high precision and accuracy. This technology allows the manipulation or modification of crop plant genome in several ways: (i) by simply incorporating random mutation (insertion or deletion) through non-homologous end joining to disrupt gene(s); (ii) by generating targeted point mutations in genes using precise base editors and (iii) by a whole-gene insertion employing the cell's homology directed repair pathway. The CRISPR-Cas technology is faster, cheaper, precise and highly efficient in editing genomes even at the multiplex level. Application of CRISPR-Cas in editing the crop plant genome is emerging rapidly for the increasing yield, quality, domestication and stress tolerance. More importantly, this technology is becoming a user-friendly tool for the development of non-transgenic genome edited crop plants to counteract harmful effects from climate change. A good number of plants edited by CRISPR-Cas technology have received free pass as non-genetically modified organism (GMO) for cultivation in the USA, and many countries including Japan are ready to follow the same. This review updates current knowledge and potentials of CRISPR-Cas9 technology for modifying the genomes of cultivated food crops to achieve food and nutritional security through resiliency against emerging pests and abiotic stresses.
Urban kampung and household energy consumption in Bandung, Indonesia
Authors: Prilandita N , Purwaningati S U , Indradjati P N 
Published: December 2019

Abstract
This paper aims to understand the energy consumption of a type of urban form known as urban kampung in Bandung, Indonesia. The high building density characteristic of urban kampung often considered as a more energy efficient urban form. This paper tries to explore it by comparing the energy consumption and behaviour of people living in urban kampung with the energy consumed by those who live in organised settlements in form of vertical housing/flats (rumah susun). A questionnaire survey was conducted to 200 households living in the three neighbourhoods in Bandung, which represented the two types of urban form. The preliminary result shows that the energy consumption of urban kampung is slightly lower but somewhat similar compared with the vertical housing. The parcel size are probably the factor that affected this result, since the other components such as electricity bill, cooking fuel, and transportation expenditure do not express any differece within the two types of urban form. This study provides insight for policy-maker and city planners on the benefit of urban kampung to achieve a more energy efficient city. The city government might consider the strategy of revitalising and improving the urban kampung before converting them into vertical housing.