bluuebunny/crossref_metadata_2025_split huggingface dataset:
Parental Knowledge about Healthy Sleep Hygiene practices in Children under 6 Year Old: an Exploratory Study
Authors: Fonseca G , Marques AC , Vidal DG , Pontes M , Martins MA 
Published: June 2020

Abstract
Introduction Children aged 5 to 12 years old needs, at least, 10-11 hours of sleep. Nowadays, children under 6 years old become more interested in TV, computers and in Internet which can lead to difficulty falling asleep. Parent knowledge about child sleep hygiene can influence parent capacity to promote healthy sleep habits, and should be supported by accurate information.
Objectives To analyse parents knowledge of children sleep practices.
Methodology A convenience sample of parents (n = 201) of children from three schools in a northern city of Portugal completed the "Sleep Ideas" Parent Survey on child sleep habits and parental basic sleep knowledge, beliefs and attitudes regarding sleep as a health behaviour.
Results Of the 201 analysed surveys (response rate 67 %), 33.8 % are from parents of children aged under 3 years old, 19.4 % of children with 3 years older, 24.4 % of children with 4 years old, 16.4 % of children with 5 years old and 6.0 % of children with 6 years old. In general, the percentage of correct answers was 84.8 %. Regarding children age, the percentage of correct answers ranged between 77.9 to 86.2 %, being the parents of children with 6 years old those who had the highest percentage of incorrect answers (22.1 %) and, on the other hand, the parents of children with 3 years old those who had the lowest percentage of incorrect answers (13.8 %). The answer with the highest percentage of incorrect answers, in all parents, was “Making a physical effort before going to sleep makes falling asleep easier” (46.8 %).
Conclusion In this study, parents revealed a very reasonable knowledge about sleep, which suggests a good capability to influence positively children’s habits. Parents with high sleep knowledge are more able to promote healthy sleep hygiene habits. Further studies are warranted to promote a better understanding of all the determinant dimensions for good sleeping habits.
Occupational Contamination with Cyclophosphamide in Manipulators
Authors: Martins S , Moreira Z 
Published: June 2020

Abstract
Introduction Cyclophosphamide is a cytotoxic widely used in the treatment of various cancers. It has been observed, for many years, that those responsible for its handling and administration are exposed and levels of contamination have been detected in biological samples collected from these professionals, in surfaces and in the air.
Objectives To review the literature on occupational contamination by cyclophosphamide.
Methodology The following inclusion criteria were selected: articles published until the present year, articles in English, scientific papers on cyclophosphamide contamination in hospital health professionals, scientific articles on contamination detection methods and articles on the effects that can outcome from cyclophosphamide contamination.
Results The cyclophosphamide levels have been decreasing with the implementation of preparation and cleaning guidelines as well as with the emergence of new techniques of manipulation and technological innovations. However, the dermal route remains the main route of contamination and those responsible for cytotoxic manipulation are not the only ones exposed. It was verified that hospital professionals, who in their profession would not be in contact with cyclophosphamide, also presented levels of contamination in the collected urine samples.
Conclusion
It is necessary to continue to alert hospital professionals to the importance of always complying with the handling and cleaning protocols, since one of the main causes of contamination is precisely the performance of incorrect procedures during both tasks. This is a topic that should be further studied in order to minimize the exposure and consequently the associated risks.
Repercussions of a very active life style in body composition and cardiometabolic parameters of the elderly in a sample of the population of the mid region of the city of recife/Brazil
Authors: Coriolano J , Queiroz W , Andrade K , Coriolano M 
Published: June 2020

Abstract
Introduction Aging is accompanied by changes in body composition and cardio metabolic parameters, which indicate the onset of chronic degenerative diseases such as cardiovascular, coronary and metabolic diseases. The prevention of these diseases in the elderly may depend on changes in risk factors related to life style, with physical activities as one of the most important components for a healthy life style.
Objectives To assess the impact of a very active life style in body composition and cardio metabolic parameters in a sample of the elderly population in the city of Recife.
Methodology A cross-sectional study conducted with the elderly (age ≥ 60 years) that were allocated into two groups of sedentary life style, and very active, according to the variable "physical exercise" of the table of coronary risk (CR) of the Michigan Heart Association, which considers the recreational and occupational effort played by the individual. The variables related to body composition were body weight, body fat percentage (%F), lean body mass, fat mass and body mass index (BMI). The cardio metabolic parameters were obtained from the CR, blood pressure and casual blood glucose. The independent T test and Fishers exact test were used, considering P &lt; 0.05.
Results The sample consisted of 363 elderly people (156 men and 207 women), 61 being very active and 302 sedentary. A significant part of the very active elderly (p = 0.001) was between 60 and 69 years (n = 41 - 69%), and none of the elderly aged ≥ 80 years (n = 21) was categorized as very active. The very active elderly presented body mass (p = 0.01), %F (p = 0.005), fat mass (p = 0.0007), BMI (p = 0.005), casual blood glucose levels (p = 0.003) and CR (p &lt; 0.0001) significantly lower than the sedentary elderly.
Conclusion The elderly with very active life style have body composition and cardio metabolic parameters better than the sedentary elderly of the sample.
Shift Work: Work ability and quality of life of professionals from IPSS
Authors: Rito J , Pereira J , Figueiredo JP , Cotrim T , Simões H 
Published: June 2020

Abstract
Introduction Shift work can influence the health and well-being of individuals due to changes in biological rhythms, causing psychological and physical disruption, hampering family relationship, social and work ability. However, the shift work of auxiliary of direct / educational action of Private Social Solidarity Institution (PSSI) is essential to ensure continuity in the provision of the best hygienic and health care to the users of these institutions every day.
Objectives The objective of this study was to evaluate the quality of life and the work capacity of Direct-Action Assistants (n = 29).
Methodology Respondents were subjected a monitoring of the heart function PB – Blood Pressure for 24 hours and answered a questionnaire (Work Ability Index and Quality of Life Index).
Results Of the 29 workers evaluated 90% were women and 10% men, aged 45.5 (± 10.47) years and exercise time of 7.1 (± 3.18) years. Physical examination revealed a mean 24 h Systolic Blood Pressure of 119.3 (± 11.8) mm Hg and 24 h Diastolic Blood Pressure of 72.6 (± 7.3) mm Hg. The mean Body Mass Index (BMI) (kg / m2) was 25.6 (± 3.5), and 24.1% of the participants were overweight and 13.7% obesity grade I. From this analysis, we found that overweight individuals have a tendency to increase the Blood Pressure compared to normal weight individuals. Although the working class has a good ability to work 69%, they are not optimistic about the future.
Conclusion We concluded that workers can be adjusted the constant changes of schedules established by their institutions, and are able to carry out their work, however, individuals with 6 or more years of work in the present-day regime shifts already feel some symptoms of physical and psychological wear, and thus speeding up their aging.
Asbestos Exposure and Malignant Pleural Mesothelioma in Portugal: a scoping review
Authors: Santos C , Dixe MA , Sacadura-Leite E , Astoul P , Sousa-Uva A 
Published: June 2020

Abstract
Introduction Asbestos, widely used for its important chemical and physical characteristics, are recognized in all their varieties as a human carcinogen by the International Agency for Research on Cancer (IARC) and as the leading cause of cancer associated with occupational exposure in industrialized countries. Despite being the most studied occupational hazard since 1965, it is the malignant pleural mesothelioma (MPM), the decisive aspect in the study of the exposure to asbestos.
Objectives Make a state of the art of the relationships between asbestos and MPM in Portugal.
Methodology A scoping review using the Joanna Briggs Institute (JBI) methodology was conducted using five information’s sources: Pubmed, Web of Science, The Cochrane Library, Scopus and Google Scholar and Open Access Scientific Repositories of Portugal and DART-Europe E-theses Portal to search also for gray literature. Data were collected between 1960-2019, with the keywords "Asbestos" and "Mesothelioma” and “Portugal”. Inclusion criteria were defined for types of participants, concept, context and types of studies.
Results Of the 1453 studies reviewed 9 were included. The oldest study is from 1986. Five studies are about incidence and survival, two are case reports, one about exposure and mortality and one about treatment. To analyse incidence and mortality, four authors used hospital data and two the data from the southern regional cancer registry (ROR). In these studies, it is also presented the description of the population in terms of mean age, type of exposure, latency time, histological type and stage.
Conclusion Portugal, such as other industrialized countries, used asbestos in a massive way, but despite this, few studies and data on the relationship between asbestos exposure and MPM are found. Dedicated studies are needed to objectively assess the true dimension of this potential problem in Portugal and characterise the different types of exposures related to MPM.
Evaluation of MMP-10 and TIMP-1 levels associated with Resveratrol supplementation
Authors: Silva AM , Ferreira C , Silva I , Clemente M , Figueiredo JP , Pereira T , Gabriel A , Caseiro A 
Published: June 2020

Abstract
Introduction Resveratrol (RSV) is a natural phytoalexin present in abundance in grapes, berries, peanuts and red wine. The main benefits of resveratrol ingestion are due to its anti-inflammatory and antioxidant properties. This compound also induces therapeutic benefits in the remodelling of extracellular matrix (ECM), which plays an important role in the regulation of the activity of matrix metalloproteinases (MMPs) and its tissue inhibitors of metalloproteinases (TIMPs). The MMPs are a family of zinc dependent endopeptidases, with an important role in the physiological and pathological remodelling of ECM, once the activity of MMPs can be regulated by TIMPs. This regulation is essential, since if there is an imbalance between the MMPs and TIMPs there’s destruction of the ECM, leading to the development of several pathologies.
Objectives Evaluate RSV potential by determining serum and salivary levels of MMP-10 and TIMP-1.
Methodology The study population included 27 undergraduates between the ages of 18 and 30, divided into a control group (placebo) and an intervention group, supplemented with 100 mg RSV/day, during 30 days. MMP-10 and TIMP-1 levels were determined by slot blot. The results were analysed using a GraphPad Prism version 5 software for Windows (GraphPad Software, San Diego, California, USA).
Results It was verified the existence of a linear correlation between the serum and salivary levels of TIMP-1, as well as a tendency of increase of this biomarker after RSV supplementation. In all indicators studied, supplementation with this compound wasn’t harmful.
Conclusion The rising trend verified at TIMP-1 in both fluids may reflect benefits in the individuals under study, demonstrating that saliva has potential as a study biofluid for this type of biochemical markers. It was also verified that supplementation with this compound did not damage the evaluated parameters and, therefore, the RSV potential shouldn’t be rejected.
Influence of Food Education on the quality of snacks of middle school children
Authors: Andrade D 
Published: June 2020

Abstract
Introduction The window of opportunity for the creation of eating behaviours and preferences, occurs during the early years, and will be reflected throughout the rest of their lives. The school represents an ideal environment to apply behavioural shaping strategies and to create eating habits, but can also be negatively affected by unbalanced eating behaviours. Cognitive performance is influenced by their diet, and a deficit of important nutrients harms school productivity, enhancing the importance of creating habits of making healthy, balanced and complete snacks.
Objectives To evaluate the quality of middle school children snacks, and to comprehend the influence of Food Education on the improvement of this same quality
Methodology Make a detailed initial registration of the snacks composition (morning and afternoon snacks), for 5 consecutive days. Then, apply lectures of Food Education throughout the school year, enhancing what is the ideal composition of these snacks. Repeat the registration of the school snacks, at the end of the year, in order to compare the data gathered before and after the Food Education qualification.
Results Through comparing the initial and final registration, it’s possible to see differences in the quantity of some food groups: grain bread (4% to 7%), even though refined bread also increased (51% to 53%); plain milk (29% to 37%), in detriment to flavoured milk (10% to 4%); sugared cakes and cookies, with or without filling (23% to 6%); industrial pastry shop products (27% to 7%).
Conclusion Based on the results gathered, it is possible to conclude that a continuous Food Education, focused on improving school snacks, led to a significant improvement of the nutritional quality of food choices, enhancing the importance and need of instruct children during their early school years, involving the environment of their everyday lives.
Inflammation disrupts epithelial barrier function and induces the release of different populations of exosomes
Authors: Martins B , Rodrigues T , Ramalho J , Ambrósio A , Girão H , Fernandes R 
Published: June 2020

Abstract
Introduction Age-related macular degeneration (AMD) is a degenerative retinal disease that affects central vision. Most of their phenotypical features are believed to be associated with the dysfunction of retinal pigment epithelium (RPE). The accumulation of damaged proteins in aged RPE is associated with disruption of proteolytic pathways and exocytic activity, with release of intracellular proteins via exosomes (Exo), that are important players in intercellular communication and can contribute to disease progression. However, the impact of their secretion by polarized RPE on outer blood retinal barrier (oBRB) breakdown remains largely elusive
Objectives Our aim was to explore the role of inflammation on the loss of RPE integrity and to understand the relative role of directional secretion of Exo by RPE in the loss of polarity and barrier disruption
Methodology We used a human RPE cell line (ARPE-19), highly polarized RPE primary cultures (pRPE) and porcine eyecups. To mimic the inflammatory conditions present in AMD, cells were treated with two inflammatory stimuli, TNF (10 ng/mL) or LPS (100 ng/mL)
Results TNF and LPS do not affect the viability of the RPE cells. RPE cells developed a confluent monolayer and reached a relatively constant TER of about 40 Ω/cm2 (ARPE-19) or higher than 150 Ω/cm2 (pRPE). Treatment with TNF significantly reduces the TER, decreased immunoreactivity and co-localization of the TJ proteins ZO-1 and occludin and increases MMP-2/-9 activity in the medium. Apical Exo isolated from the RPE cells are enriched in CD63 compared to the basolateral Exo, that are enriched in CD81. The Exo isolated from porcine eyecups, especially with the LPS stimulus, are enriched in CD81 and MMP-2 but have similar levels of CD63
Conclusion Overall, our results show that inflammation induces loss of RPE integrity and release of different populations of Exo. The unravelling of novel drug targets paves the way for development of new therapeutic strategies for AMD.
My health, myself: a qualitative study on motivations for effective chronic disease self-management among community dwelling adults
Authors: Hadi Muhammad Danial , Lin Yongxing Patrick , Chan Ee-Yuee 
Published: June 2020

Abstract

Background
Chronic diseases continue to be a significant cause of morbidity and mortality despite modifiable risk factors. This suggests that current primary healthcare provision needs to delve beyond patient education, to understand the motivators that drive patients to undertake chronic disease self-management. Understanding these motivations within the context of a multi-cultural community can facilitate tailored support for chronic disease self-management.

Objectives
To explore the motivations behind effective chronic disease self-management in community dwelling adults in Singapore.

Methods
A qualitative descriptive study was carried out in five clinics in a large medical centre. Twelve participants who were assessed to be optimally managing their chronic diseases were recruited using purposive sampling. Individual in-depth interviews were conducted until data saturation, with data thematically analysed.

Results
Three salient themes emerged from the data. Firstly, ‘Regaining self-control, avoiding complications’ describes the participants’ journey towards personal mastery in self-management, as well as a fear of debilitating complications resulting in their desire for good health. Secondly, ‘Preserving social identities and roles’ illustrates how participants yearn to maintain their pre-existing roles and functions through maintenance of their health. Finally, ‘Accessing proximal support systems’ highlights resources and supports surrounding the participants that encourage continued chronic disease self-management. Within each theme, specific motivators and challenges encountered by participants in their self-management journey were discussed.

Conclusions
Findings can prompt primary healthcare providers to leverage on the patients’ life goals and social roles in chronic disease self-management support. This may empower patients to engage in self-management and strengthen primary care provision.
Positive SARS-CoV-2 RNA recurs repeatedly in a case recovered from COVID-19: dynamic results from 108 days of follow-up
Authors: Liu Fang , Cai Zhao-bin , Huang Jin-song , Yu Wen-yan , Niu Hai-ying , Zhang Yan , Sui Dong-ming , Wang Fei , Xue Li-zhi , Xu Ai-fang 
Published: June 2020

ABSTRACT
The evidence of long-term clinical dynamic on Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) RNA re-positive case are less. We performed a 108 days follow-up on dynamic clinical presentations in a case, who hospitalized three times due to the positive recurrence of SARS-CoV-2 RNA after discharge, to understand the prognosis of the 2019-Coronavirus disease (COVID-19). In this case, positive SARS-CoV-2 recurred even after apparent recovery (normal CT imaging, no clinical symptoms, negative SARS-CoV-2 on stool sample and negative serum IgM test) from COVID-19, viral shedding duration lasted for 65 days, the time from symptom onset to disappearance was up to 95 days. Erythrocyte-associated indicators, liver function and serum lipid metabolism presented abnormal throughout during the observation period. Awareness of atypical presentations such as this one is important to prompt the improvement of the management of COVID-19.
Constraining 1-D inner core attenuation through measurements of strongly coupled normal mode pairs
Authors: Talavera-Soza S , Deuss A 
Published: June 2020

SUMMARYWe measured inner core normal mode pair 10S2–11S2, which cross-couples strongly for 1-D structure and is sensitive to shear wave velocity, and find that our measurements agree with a strongly attenuating inner core. In the past, this mode pair has been used to try to resolve the debate on whether the inner core is strongly or weakly attenuating. Its large spectral amplitude in observed data, possible through the apparent low attenuation of 10S2, has been explained as evidence of a weakly attenuating inner core. However, this contradicted body waves and other normal modes studies, which resulted in this pair of modes being excluded from inner core modelling. Modes 10S2 and 11S2 are difficult to measure and interpret because they depend strongly on the underlying 1-D model used. This strong dependence makes these modes change both their oscillation characteristics and attenuation values under a small 1-D perturbation to the inner core model. Here, we include this effect by allowing the pair of modes to cross-couple or resonate through 1-D structure and treat them as one hybrid mode. We find that, unlike previously thought, the source of 10S2 visibility is its strong cross-coupling to 11S2 for both 1-D elastic and anelastic structure. We also observe that the required 1-D perturbation is much smaller than the 2 per cent vs perturbation previously suggested, because we simultaneously measure 3-D structure in addition to 1-D structure. Only a 0.5 per cent increase in inner core vs or a 0.5 per cent decrease in inner core radius is required to explain 10S2–11S2 observations and a weakly attenuating inner core is not needed. In addition, the 3-D structure measurements of mode 10S2 and its cross-coupling to 11S2 show the typical strong zonal splitting pattern attributed to inner core cylindrical anisotropy, allowing us to add further constrains to deeper regions of the inner core.
Reliable and sensitive detection of glycosaminoglycan chains with immunoblots
Authors: Nagase Haruna , Higashi Sayuri L , Iweka Chinyere A , Pearson Craig S , Hirata Yoko , Geller Herbert M , Katagiri Yasuhiro 
Published: June 2020

Abstract
Complex glycans play vital roles in many biological processes, ranging from intracellular signaling and organ development to tumor growth. Glycan expression is routinely assessed by the application of glycan-specific antibodies to cells and tissues. However, glycan-specific antibodies quite often show a large number of bands on immunoblots and it is hard to interpret the data when reliable controls are lacking. This limits the scope of glycobiology studies and poses challenges for replication. We sought to resolve this issue by developing a novel strategy that utilizes an immunoreaction enhancing technology to vastly improve the speed and quality of glycan-based immunoblots. As a representative case study, we used chondroitin sulfate glycosaminoglycan (CS-GAG) chains as the carbohydrate target and a monoclonal antibody, CS-56, as the probe. We discovered that preincubation of the antibody with its antigenic CS-GAG chain distinguishes true-positive signals from false-positive ones. We successfully applied this strategy to 10E4, a monoclonal anti heparan sulfate GAGs (HS-GAGs) antibody, where true-positive signals were confirmed by chemical HS-GAG depolymerization on the membrane. This evidence that glycan-specific antibodies can generate clear and convincing data on immunoblot with highly replicable results opens new opportunities for many facets of life science research in glycobiology.