bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quantitative nanoscale measurements of the thermomechanical properties of poly‐ether‐ether‐ketone (PEEK)
Authors: Qian Wen , Li Wenlong , Nguyen Charles , Johnson Tyler J. , Turner Joseph A. 
Published: April 2020

AbstractUnderstanding the internal structure and organization of semicrystalline polymers, especially at the nanoscale, has many challenges for researchers to date. In this article, we demonstrate a quantitative method for investigating the local viscoelastic properties (i.e., storage and loss moduli, as well as loss tangent) of semicrystalline polyether‐ether ketone (PEEK) through the combination of contact resonance atomic force microscopy (CR‐AFM) and in situ local heating with a thermal probe. Furthermore, the local viscoelastic properties of the crystalline and amorphous phases were decoupled by performing thermal CR‐AFM array mapping near the glass transition temperature of PEEK (Tg, 143 °C). A distinct bimodal distribution of tip‐sample interaction was observed for PEEK near its Tg, providing a means to estimate the Tg and the degree of crystallinity of PEEK.
Pre-exposure Prophylaxis (PrEP) for HIV Prevention Among Men Who Have Sex with Men (MSM): A Scoping Review on PrEP Service Delivery and Programming
Authors: Hillis Alyson , Germain Jennifer , Hope Vivian , McVeigh James , Van Hout Marie Claire 
Published: April 2020

AbstractPre-exposure prophylaxis (PrEP) is an evidence-based new biomedical HIV prevention intervention, which involves the pre-emptive use of daily (or event-based) antiretroviral drugs, to reduce risk of HIV acquisition if exposed. PrEP has recently been positioned as an integral prevention tool to reduce HIV acquisition risk among men who have sex with men (MSM) at country-level and within global prevention strategies. Given this global scale up of PrEP, we conducted a scoping review of extant international literature documenting service related perspectives, models and lessons learnt in PrEP programming for MSM. A systematic search of literature was conducted, and restricted to English language records in the timeframe 2008 to February 2019. Eligibility criteria centered on whether studies broadly described PrEP programming and service delivery for MSM as well as health communication. Following exclusion of ineligible records and removal of duplicates, 84 records were charted and thematically analysed according to scoping review methods. Four themes emerged from the thematic analysis of data; ‘PrEP service aspects, settings and staff’; ‘PrEP prescriber experiences, therapeutic alliance and care planning’; ‘PrEP adherence within formal service structures’; and ‘Multi-disciplinary and innovative PrEP care pathways’. The review highlights the complexities in providing optimal PrEP services for MSM by mapping and illustrating the importance of understanding the informal and formal routes to PrEP use among this HIV risk population; the barriers to uptake; the requirement for the presence of a positive therapeutic alliance between patient and prescriber in supporting patient initiation and adherence to PrEP regimes; and the need for availability in different culturally and ethnically sensitive models of PrEP service delivery according to low to high risk groups within the MSM communities.
Physicochemical and dissolution properties of ezetimibe–aspirin binary system in development of fixed-dose combinations
Authors: Górniak Agata , Czapor-Irzabek Hanna , Złocińska Adrianna , Karolewicz Bożena 
Published: April 2020

AbstractThe objective of this work was to investigate binary pharmaceutical mixtures of ezetimibe (EZT) and aspirin (ASA) in order to identify whether the occurrence of eutectic in this system has an effect on EZT dissolution improvement. Ezetimibe–aspirin (EZT–ASA) solid dispersions prepared by grinding in the whole range of compositions were characterized using differential scanning calorimetry (DSC) for purpose to describe solid-liquid phase equilibrium diagram. The occurrence of interactions between ingredients was excluded by Fourier transform infrared spectroscopy and X-ray powder diffractometry. Dissolution studies have shown that the mixtures containing from 10 to 60 mass% of EZT (53.5 mass% of EZT in eutectic composition) have released ezetimibe faster than a sample of pure drug. Moreover, ASA is released more quickly from all obtained dispersions than from powder alone. Our studies have shown that obtained mixtures are useful to obtain the fixed-dose combinations, capable to deliver these two APIs together in a single system with enhanced dissolution of EZT and ASA.
The epistemic significance of modal factors
Authors: Newton Lilith 
Published: April 2020

AbstractThis paper evaluates whether and to what extent modal constraints on knowledge or the semantics of ‘knows’, which make essential reference to what goes on in other possible worlds, can be considered non-epistemic factors with epistemic significance. This is best understood as the question whether modal factors are non-truth-relevant factors that make the difference between true belief and knowledge, or to whether a true belief falls under the extension of ‘knowledge’ in a context, where a factor is truth-relevant with respect to S’s belief that P iff it bears on the probability that P is true. To the extent that these factors are non-epistemic, epistemologies that endorse them—modal epistemologies—stand in conflict with intellectualism. I focus on three modal epistemologies: safety, sensitivity, and David Lewis’s epistemic contextualism. I argue that prima facie, safety and sensitivity allow that non-epistemic changes in a context can shift the closeness ordering on worlds, and in so doing make a difference to whether S knows P, while Lewis’s contextualism allows that non-epistemic changes in a context can shift the relevant domain of not-P possibilities that must be eliminated for ‘S knows P’ to be true in that context. Then to make her theory compatible with intellectualism, the modal epistemologist must say much more about the notion of probability at play in the definition of ‘truth-relevant’. I suggest that either accepting or rejecting that modal epistemologies are intellectualist has significance consequences for debates between pragmatists and purists, which radiate into wider contemporary epistemology.
Changes in functional, phylogenetic and taxonomic diversities of lowland fens under different vegetation and disturbance levels
Authors: Carvalho Fabio , Brown Kerry A. , Waller Martyn P. , Razafindratsima Onja H. , Boom Arnoud 
Published: April 2020

AbstractMultiple measures of plant diversity are vital to understand the response of plant communities to changing environmental conditions in peatlands. We assessed whether functional, phylogenetic and taxonomic diversities of woody and herbaceous fen peatlands in East Anglia, UK varied between plant communities under different management practices (mowing and grazing). We adjusted the weight of phylogenetic distances in a combined functional-phylogenetic distance matrix to assess functional and phylogenetic diversities separately and in combination. We tested the phylogenetic signal of four traits (leaf dry-matter content, leaf N, leaf δ13C and leaf δ15N) and employed null models to determine patterns of clustering and over-dispersion of traits and phylogenies. We used rarefaction to determine if observed taxonomic diversity was higher or lower than expected. Functional, phylogenetic and taxonomic diversities varied across and within vegetation types. Annual grazing was associated with reduced functional and phylogenetic diversities but was not significantly associated with taxonomic diversity. Annual mowing was associated with increased phylogenetic and taxonomic diversities. Multiple diversity metrics can provide complementary or contrasting information. While there are benefits to annual management of wetlands (benefitting rare species), these must be weighed against eroding functional and phylogenetic diversities that can potentially adversely affect responses to environmental change. Communities mown every seven to eight years supported characteristic fen vegetation and maintained high plant diversity across a range of measures. Our results sound a cautionary note of neglecting to monitor multiple plant diversity measures in managed habitats, since attempts to maximize one may inadvertently lead to the erosion of others.
Financial Reporting in Non-listed Family Firms: Insights from Interviews with CFOs
Authors: Glaum Martin 
Published: April 2020

AbstractAnalysing 20 interviews with CFOs of German non-listed family firms, I report how the firms’ financial reporting is organised, who are the financial statement addressees, how the CFOs define accounting quality, whether they manage earnings, and how owner families influence reporting. I also discuss the firms’ costs of reporting and disclosure. Most CFOs see the requirement to disclose financial statements as a burden. They react by disclosing statements as late as possible and by providing only the minimum content necessary. As regards accounting quality, the CFOs value formal correctness, but also sustainability, persistence, and conservative estimation of net income. Most CFOs engage in earnings management, above all to achieve a positive trend in net income, in some cases also to comply with debt covenants. The influence of the owner families differs; however, in most cases the family at least “sets the tone” for the reporting. On average, the direct costs of accounting and auditing in my sample firms amount to about 1% of revenues. According to the CFOs, public disclosure does not result in material indirect costs, and most CFOs would not change much if the requirements for reporting and disclosure were abolished. These findings add to our understanding of private firm financial reporting and suggest several directions for future research.
Narnaviruses: novel players in fungal–bacterial symbioses
Authors: Espino-Vázquez Astrid N , Bermúdez-Barrientos J Roberto , Cabrera-Rangel J Francisco , Córdova-López Gonzalo , Cardoso-Martínez Faviola , Martínez-Vázquez Azul , Camarena-Pozos David A , Mondo Stephen J , Pawlowska Teresa E , Abreu-Goodger Cei , Partida-Martínez Laila P 
Published: April 2020

Abstract
Rhizopus microsporus is an early-diverging fungal species with importance in ecology, agriculture, food production, and public health. Pathogenic strains of R. microsporus harbor an intracellular bacterial symbiont, Mycetohabitans (formerly named Burkholderia). This vertically transmitted bacterial symbiont is responsible for the production of toxins crucial to the pathogenicity of Rhizopus and remarkably also for fungal reproduction. Here we show that R. microsporus can live not only in symbiosis with bacteria but also with two viral members of the genus Narnavirus. Our experiments revealed that both viruses replicated similarly in the growth conditions we tested. Viral copies were affected by the developmental stage of the fungus, the substrate, and the presence or absence of Mycetohabitans. Absolute quantification of narnaviruses in isolated asexual sporangiospores and sexual zygospores indicates their vertical transmission. By curing R. microsporus of its viral and bacterial symbionts and reinfecting bacteria to reestablish symbiosis, we demonstrate that these viruses affect fungal biology. Narnaviruses decrease asexual reproduction, but together with Mycetohabitans, are required for sexual reproductive success. This fungal–bacterial-viral system represents an outstanding model to investigate three-way microbial symbioses and their evolution.
Comparative lipidomics of 5-Fluorouracil–sensitive and –resistant colorectal cancer cells reveals altered sphingomyelin and ceramide controlled by acid sphingomyelinase (SMPD1)
Authors: Jung Jae Hun , Taniguchi Kohei , Lee Hyeong Min , Lee Min Young , Bandu Raju , Komura Kazumasa , Lee Kil Yeon , Akao Yukihiro , Kim Kwang Pyo 
Published: April 2020

Abstract5-Fluorouracil (5-FU) is a chemotherapeutic drug widely used to treat colorectal cancer. 5-FU is known to gradually lose its efficacy in treating colorectal cancer following the acquisition of resistance. We investigated the mechanism of 5-FU resistance using comprehensive lipidomic approaches. We performed lipidomic analysis on 5-FU–resistant (DLD-1/5-FU) and -sensitive (DLD-1) colorectal cancer cells using MALDI-MS and LC-MRM-MS. In particular, sphingomyelin (SM) species were significantly up-regulated in 5-FU–resistant cells in MALDI-TOF analysis. Further, we quantified sphingolipids including SM and Ceramide (Cer) using Multiple Reaction Monitoring (MRM), as they play a vital role in drug resistance. We found that 5-FU resistance in DLD-1/5-FU colorectal cancer cells was mainly associated with SM increase and Cer decrease, which are controlled by acid sphingomyelinase (SMPD1). In addition, reduction of SMPD1 expression was confirmed by LC-MRM-MS analysis and the effect of SMPD1 in drug resistance was assessed by treating DLD-1 cells with siRNA-SMPD1. Furthermore, clinical colorectal cancer data set analysis showed that down-regulation of SMPD1 was associated with resistance to chemotherapy regimens that include 5-FU. Thus, from our study, we propose that SM/Cer and SMPD1 are new potential target molecules for therapeutic strategies to overcome 5-FU resistance.
High predation of native sea lamprey during spawning migration
Authors: Boulêtreau Stéphanie , Carry Laurent , Meyer Elise , Filloux Damien , Menchi Olivier , Mataix Vincent , Santoul Frédéric 
Published: April 2020

AbstractSea lamprey (Petromyzon marinus) is a unique jawless vertebrate among the most primitive of all living vertebrates. This migratory fish is endangered in much of its native area due to dams, overfishing, pollution, and habitat loss. An introduced predator, the European catfish (Silurus glanis), is now widespread in Western and Southern European freshwaters, adding a new threat for sea lamprey migrating into freshwater to spawn. Here, we use a new prototype predation tag coupled with RFID telemetry on 49 individuals from one of the largest sea lamprey European populations (Southwestern France) to quantify the risk of predation for adult sea lampreys during its spawning migration in rivers with large populations of European catfish. We found that at least 80% of tagged sea lampreys (39 among 49) were preyed upon within one month, and that 50% of the released lampreys were rapidly consumed on average 8 days after tagging. This very high predation rate suggests that the European catfish represents a supplementary serious threat of extirpation for the native sea lamprey population we studied. This threat is likely to happen throughout most of the native lamprey distribution area, as the European catfish is becoming established almost everywhere the sea lamprey is.
Pedobacter schmidteae sp. nov., a new bacterium isolated from the microbiota of the planarian Schmidtea mediterranea
Authors: Kangale Luis Johnson , Raoult Didier , Ghigo Eric , Fournier Pierre-Edouard 
Published: April 2020

AbstractPedobacter schmidteae sp. nov. strain EGT(Collection de Souches de l’Unité des Rickettsie CSUR P6417 = Colección Española de Cultivos Tipo CECT 9771) is a newPedobacterspecies isolated from the planarianSchmidtea mediterranea.Schmidtea mediterraneaare flatworms living in freshwater and exhibiting an unusual ability to regenerate amputated parts. To date, the gut microbiota ofSchmidtea mediterranearemains poorly studied. Here, via the culturomics strategy that consists in using diversified culture conditions, we isolated a new bacterium, strain EG, that we characterized using the taxono-genomics approach that combines phenotypic assays and genome sequencing and analysis. Strain EG exhibits a 16S rRNA sequence similarity of 98.29% withPedobacter nyackensisstrain NWG-II14T, its closest neighbour with standing in nomenclature. It is an aerobic bacterium belonging to the familySphingobacteriaceae. Colonies are small, round, smooth and transparent. Bacterial cells are Gram-negative, rod-shaped, motile and non-spore-forming bacilli with positive catalase and oxidase activities. The genome sequence is 6,198,518 bp–long with a G + C content of 41.13%, and the Ortho-ANI and dDDH values when compared toP. nyackensisare 77.34% and 21.50%, respectively. Strain EGTexhibits unique characteristics that classify it as the type strain of new bacterial species for which we propose the namePedobacter schmidteaesp. nov.
Association between Macular Thickness Profiles and Visual Function in Healthy Eyes: The Singapore Epidemiology of Eye Diseases (SEED) Study
Authors: Poh Stanley , Tham Yih-Chung , Chee Miao Li , Dai Wei , Majithia Shivani , Soh Zhi Da , Fenwick Eva K. , Tao Yijin , Thakur Sahil , Rim Tyler Hyungtaek , Sabanayagam Charumathi , Cheng Ching-Yu 
Published: April 2020

AbstractThis study aimed to evaluate the association between optical coherence tomography (OCT)-measured retinal layer thickness parameters with clinical and patient-centred visual outcomes in healthy eyes. Participants aged 40 and above were recruited from the Singapore Epidemiology of Eye Diseases Study, a multi-ethnic population-based study. Average macular, ganglion cell-inner plexiform layer (GCIPL), and outer retinal thickness parameters were obtained using the Cirrus High Definition-OCT. Measurements of best-corrected visual acuity (BCVA) and 11-item visual functioning questionnaire (VF-11) were performed. Associations between macular thickness parameters, with BCVA and Rasch-transformed VF-11 scores (in logits) were assessed using multivariable linear regression models with generalized estimating equations, adjusted for relevant confounders. 4,540 subjects (7,744 eyes) with a mean age of 58.8 ± 8.6 years were included. The mean BCVA (LogMAR) was 0.10 ± 0.11 and mean VF-11 score was 5.20 ± 1.29. In multivariable regression analysis, thicker macula (per 20 µm; β = −0.009) and GCIPL (per 20 µm; β = −0.031) were associated with better BCVA (all p ≤ 0.001), while thicker macula (per 20 µm; β = 0.04) and GCIPL (per 20 µm, β = 0.05) were significantly associated with higher VF-11 scores (all p < 0.05). In conclusion, among healthy Asian eyes, thicker macula and GCIPL were associated with better vision and self-reported visual functioning. These findings provide further understanding on the potential influence of macular thickness on visual function.
Preoperative red cell distribution width predicts postoperative cognitive dysfunction after coronary artery bypass grafting
Authors: Wan Jing , Luo Peiwen , Du Xiaonan , Yan Hong 
Published: April 2020

Abstract
We assessed the relationship between red blodd cell distribution width (RDW) and postoperative cognitive dysfunction (POCD) after coronary artery bypass grafting (CABG) in patients who usually had obvious hemodynamic changes. We enrolled 362 coronary heart disease patients who received CABG. POCD was assessed through neuropsychological examination at 21 days after operation. Demographics, history of diseases, blood biochemical parameters and perioperative data were collected. The receiver operating characteristic (ROC) curve was used to find the best cut-off value of RDW for diagnosis of POCD. Logistic regression was used to explore the relationship between RDW and POCD. The 21-day incidence of POCD in patients receiving CABG was 27.1% (98/362). The RDW of POCD patients was significantly higher than in the non-POCD patients (17.4 vs. 13.2). The sensitivity and specificity of RDW for predicting POCD were 82.7 and 64.8%, respectively. The POCD patients also tended to be older and had higher fasting plasma glucose, hypersensitive c-reactive protein, tumor necrosis factor-α, white blood cell levels and longer surgery time. No significant differences were found in other parameters. The 21-day neuropsychological test results were better in the POCD patients than the non-POCD patients. After adjustment of potential factors, the preoperative high RDW was still associated with an increased risk of POCD (odds ratio (OR) = 2.52, 95% confidence interval (CI): 1.28–4.31). Our study indicates that preoperative RDW is significantly elevated in POCD patients receiving CABG. The elevated preoperative RDW is associated with an increased risk of POCD and preoperative RDW can be an independent predictor of POCD.
The association between Interleukin-6 rs1800795/rs1800797 polymorphisms and risk of rotator cuff tear in a Chinese population
Authors: Li Jin , Jiang Lifeng , Zhou Xindie , Wu Lidong , Li Dong , Chen Gang 
Published: April 2020

Abstract
Expression of proinflammatory cytokines, such as interleukin (IL)-6 (IL-6) and metalloproteases, are elevated in patients with rotator cuff tear (RCT). In order to investigate the role of IL-6 gene polymorphisms on RCT risk, we genotyped two SNPs on IL-6 gene (rs1800795 and rs1800797) in 138 RCT patients and 137 healthy controls using polymerase chain reaction (PCR) and Sanger sequencing. The IL-6 expression in shoulder joint synovial fluid was determined by using enzyme-linked immunosorbent assay (ELISA) method. The constant score and visual analog scale (VAS) were used to evaluate the clinical outcome of two s (surgicsal vs. conservative) for RCT patients. For rs1800795, individuals with the GG genotype or G allele had significantly higher risk of RCT. Elevated risk of tear size was associated with the GG genotype of the rs1800795 polymorphism. The IL-6 rs1800797 polymorphism was also associated with an increased risk of RCT, especially among female, drinkers, and individuals with B(MI) &lt; 25 kg/m2. The elevated levels of IL-6 gene were observed among the mutant genotype of rs1800795/rs1800797 polymorphism. Surgical group is significantly better than conservative treatment from the perspective of constant score and VAS. Furthermore, CG genotype of rs1800795 polymorphism increased the constant score at 6 months in comparison with CC genotype. In conclusion, our study supports a role of IL-6 rs1800795/rs1800797 polymorphisms on increased RCT risk. The RCT patients with CG genotype of rs1800795 polymorphism have more obvious surgical treatment effects by influencing the IL-6 expression.
Early differential diagnosis model for acute radiation pneumonitis based on multiple parameters
Authors: Wang Zhiwu , Wu Qiong , Dong Liang , Fu Haoyu , Liu Qiwei 
Published: April 2020

Abstract
Objective: The present study aimed to construct a diagnosis model for the early differentiation of acute radiation pneumonitis (ARP) and infectious pneumonitis based on multiple parameters.
Methods: The present study included data of 152 patients admitted to the Department of Radiochemotherapy, Tangshan People’s Hospital, who developed ARP (91 patients) or infectious pneumonia (IP; 61 patients) after radiotherapy. The radiophysical parameters, imaging characteristics, serological indicators, and other data were collected as independent variables, and ARP was considered as a dependent variable. Logistics univariate analysis and Spearman correlation analysis were used for selecting independent variables. Logistics multivariate analysis was used to fit the variables into the regression model to predict ARP.
Results: The univariate analysis showed that the positional relation between lesions and V20 area (PRLV), procalcitonin (PCT), C-reactive protein (CRP), mean lung dose (MLD), and lung volume receiving ≥20 Gy (V20) correlated with ARP while the planning target volume (PTV) dose marginally correlated with ARP. The multivariate analysis showed that the PRLV, PCT, white blood cell (WBC), and MLD were independent diagnostic factors. The nomogram was drawn on the basis of the logistics regression model. The area under the curve (AUC) of the model was 0.849, which was significantly better than that of a single indicator and the sensitivity and specificity of the model were high (82.4 and 82.0%, respectively). These results predicted by the model were highly consistent with the actual diagnostic results. The decision curve analysis (DCA) demonstrated a satisfactory positive net benefit of the model.
Conclusion: The diagnosis model constructed in the present study is of certain value for the differential diagnosis of ARP and IP.