bluuebunny/crossref_metadata_2025_split huggingface dataset:
Revealing the therapeutic effects of aminolevulinate mediated femtosecond laser induced photo-chemotherapy in different cancer cells
Authors: Kars Meltem Demirel , Yıldırım Gamze , Gündoğdu Yasemin , Gönce Fatmanur , Ayan Esra , Kılıç Hamdi Şükür 
Published: October 2020

Abstract
Photodynamic therapy (PDT) is a photo chemotherapeutic strategy that is the application of photosensitizing agent and light on disease or tumor site. The aim of this study is to confirm the feasibility for femtosecond (fs) laser for aminolevulinate (ALA) mediated PDT on skin, breast and bladder cancer cells. Also the remarkable aspects of ALA mediated and laser induced PDT with respect to other literally known applications were investigated.
Metastatic melanoma cells SK-MEL30, mammary epithelial carcinoma cells MCF-7 and bladder cancer cells UMUC-3 were treated with ALA and then the cells were irradiated by fs laser at thirty wavelengths in between 230 and 800 nm for 30s and 60s. Anti-cancer effects of ALA phototherapy on different cancer cell lines were determined. Protoporphyrin IX (PpIX) accumulation was visualized by confocal microscopy. The effective PDT wavelengths were applied to evaluate the degree of apoptosis and necrosis in cells.
The viability tests demonstrated that wavelengths 400-440 nm and 600-630 nm were found to decrease the viability on three model cell lines. PDT at 630 nm exerted cell death by necrosis and apoptosis after 30 s and 60 s periods.
This paper confirms that ALA and femtosecond laser mediated PDT may be used together as therapeutic and diagnostic method to target breast, skin and urinary bladder cancer cells. The use of fs laser allows the flexibility for optimization of wavelength for photosensitizing agents.
Nile Red assay development for the estimation of neutral lipids in Chlorella emersonii and Pseudokirchneriella subcapitata
Authors: Priyanka Priyanka , Kinsella Gemma K. , Henehan Gary T. , Ryan Barry 
Published: October 2020

Abstract
Fluorescent dyes offer a useful method for the measurement of intracellular lipids. They are inexpensive and require simple optical measurement instrumentation, whilst simultaneously providing high throughput application. Nile Red is a hydrophobic, metachromatic dye which has been widely used for detection of intracellular lipids. However, Nile Red fluorescence depends on its concentration, microenvironment polarity, incubation time and, therefore, requires strain specific optimization. Hence, neutral lipids in Chlorella emersonii and Pseudokirchneriella subcapitata cannot be quantified using existing Nile Red methods developed for other microalgae strains and, therefore an optimised procedure for these strains is required. In this method development, the optimal excitation and emission wavelengths were selected based on the solvent used for Nile Red dissolution. The effect of Nile Red concentration, microalgae cell concentration, incubation time on fluorescence intensity was explored and optimised. Quintuplet assay repeats were executed for increased assay robustness for two microalgae strains, Chlorella emersonii and Pseudokirchneriella subcapitata, with protocol reliability confirmed by fluorescence microscopy. In brief, 20% (v/v) DMSO containing 10μg/ml and 5μg/ml Nile red was found to be ideal concentration for neutral lipid estimation in Chlorella emersonii and Pseudokirchneriella subcapitata respectively when an incubation time of 60mins and 40mins at 40°C was used. This optimised Nile Red protocol is a robust, simple and cost-effective method for neutral lipid quantification in Chlorella emersonii and Pseudokirchneriella subcapitata.
Optimization of Lyophilized LAMP and RT-PCR Reaction Mixes for Detection of Tuberculosis
Authors: Agel Esra , Sagcan Hasan 
Published: October 2020

Abstract
Undoubtedly, one of the most infectious diseases in the world is tuberculosis. Key factor for tuberculosis control is to prevent possible contagion with rapid diagnosis and effective treatment. The culture method, which it takes several weeks to obtain results, is the gold standard method for laboratory diagnosis of tuberculosis. In order to prevent possible contagion of tuberculosis, diagnosis must be made in short time and treatment should be started as soon as possible. Normally, clinical samples are studied in advanced laboratories designed for this purpose. However, especially after the screening in rural areas, the transmission of the samples to the centers has many negative effects on the clinical material. Therefore, the latest trend molecular techniques in microbiological diagnosis are developing into point of care systems that can be applied in the field without laboratory infrastructure. The major challenge for molecular-based point-of-care tests is the need to store polymerase enzymes and some of the ingredients used in the cold chain. The aim of this study is to increase the resistance of the amplification reaction mixtures by lyophilizing the tuberculosis diagnosis. Lyophilization was performed on Loop-mediated isothermal amplification (LAMP) and Real-time PCR mixtures. For the lyophilization of LAMP and RT-PCR mixtures, two different experimental setups were tried from the literature except for the developed content. Chemicals such as stachyose, trehalose, glycerol and PEG 8000 are widely using as cryoprotectants. As a result, the developed content (0.5% PEG 8000, 2.0 % Stachyose) was determined the best cryoprotectant mixture. Accordingly, amplification mixtures can be produced with the developed lyophilization method and point of care kits can be developed.
Relationship between organisational silence and employee demographic characteristics: the case of Lithuanian teachers
Authors: Jesevičiūtė-Ufartienė Laima , Brusokaitė Greta , Widelska Urszula 
Published: October 2020

Abstract
Organisational development requires creative and open employees, who must feel confident to use their inventiveness and share ideas. However, some entities encounter organisational silence. The lack of research into this phenomenon in Lithuanian educational institutions encouraged the authors of the article to investigate how demographic characteristics of teachers relate to types of organisational silence. The authors used two nonparametric tests for analyses, i.e. Mann–Whitney U to study gender and Kruskal–Wallis H to investigate age and marital status. The quantitative research targeted teachers of 104 Lithuanian secondary schools. The research findings contribute to filling the knowledge gap in the topic of organisational silence in Lithuania. The enclosed demographic characteristics can help rectify the current situation in educational institutions.
Controlling Effectiveness Model — empirical research results regarding the influence of controlling on organisational performance
Authors: Bieńkowska Agnieszka 
Published: October 2020

Abstract
The article aims to explain how controlling influences an organisation as a whole, considering the job performance of employees and managers. It describes the development and verification of the Controlling Effectiveness Model, which characterises the impact, the place of each variable and the direction of each relationship in the effort to shape organisational performance. The hypothesis was verified with the help of empirical research, which was conducted with 264 organisations operating in Poland. The survey took place in October 2019. The authors of the article used the CAWI method. Efforts had been made to ensure a diversified research sample encompassing various organisational characteristics. The exploratory and confirmatory factor analysis and the sequentially mediated regression model were used to verify the hypothesis. The empirical research allowed confirming a statistically significant indirect impact of the quality of controlling on organisational performance. This relationship depends on the job performance of managers and employees. The analysis of the impact made by controlling on the job performance of employees and managers as we as the organisational performance resulted in a mediation model (the Controlling Effectiveness Model) and confirmed the effect of controlling on organisational performance through the impact on job performance of managers and employees. The article has practical implications. The organisations that decide to implement controlling should focus on the quality of this management support method. It is not enough to simply implement controlling as organisations need to ensure the correct implementation. In this context, it is also relevant to properly shape functional, organisational and instrumental controlling solutions (tailored to the characteristics of the organisation as a whole, as well as to the environmental conditions, under which the organisation operates), which determine the quality of controlling.
Performance and causes of development problems among Latvian grain cooperatives
Authors: Mistris Jānis , Mistre Baiba , Zvaigzne Anda 
Published: October 2020

Abstract
The research aimed to examine Latvian grain cooperatives in terms of their performance and causes of development problems. The research employed several approaches, including a monographic method, induction and deduction, comparison, graphical method, statistical analysis, cause and consequence analysis and a sociological research method in the form of structured expert survey and interviews. The research examined the theory on cooperative development in Latvia and the world, analysed the Latvian grain production industry and made a statistical analysis of the performance of grain cooperatives. The total output of cereals was affected by the total area cropped with cereals, which was proved by the correlation coefficient r = 0.90. An increase in the area used for cereals leads to an increase in the total cereal output. The correlation coefficient showed a strong relationship between independent and dependent variables. Structured interviews with experts allowed the authors to identify the factors that hinder the development of grain cooperatives in Latvia. The industry experts identified the technological factor, i.e., poorly developed agricultural processing. As possible causes of the previously identified problem, experts identified a lack of financial resources, the unclear market situation, workforce problems, additional costs, and a lack of initiative in identifying new opportunities. The expert method helped to identify the most significant problem for the development of Latvian grain cooperatives and the underlying causes. The research allows drawing the attention of policymakers to the main problem regarding the development of grain cooperatives, namely, the technological factor of underdeveloped grain processing. The cooperatives did not own processing enterprises, which was mainly due to an unclear situation in the sales market.
Moderating role of corporate reputation in the influence of external support on organisational resilience and performance
Authors: Zabłocka-Kluczka Anna , Sałamacha Anna 
Published: October 2020

Abstract
The article analyses the influence made by corporate reputation on the mediation model for the impact of external support on organisational performance through resilience. The article aims to clarify the mechanism behind the moderating role of corporate reputation played in the influence of external support on organisational performance and considering the mediating role of organisational resilience. The empirical research was made to verify the existence of the relationship and to reach the aim of the paper. The set of hypotheses was built based on the theoretical research and then verified on the sample of 268 organisations operating in Poland. Dependences among the data selected from the theoretical research were analysed using statistical methods, including the correlation with the moderator and the mediated regression analysis. The obtained results clearly showed that corporate reputation was a moderator of the discussed mediation model for the influence of the external support on organisational performance through resilience. The paper contributes to further development of knowledge in organisational resilience management. It clarifies and stresses the role of two external factors: corporate reputation and external support in shaping the resilience of an organisation and its performance. The obtained results imply specific practical recommendations. Since corporate reputation can be the key to achieving greater organisational resilience and performance, special attention should be given to managing this category in an organisation.
Biomineralogical investigation of late-harvest grapes colonised by Botrytis cinerea Pers.
Authors: Grabowski Marek , Pawlikowski Maciej 
Published: October 2020

Abstract
The ripening process of two grape varieties in the vineyard located in the Sandomierz region was examined. In 2015, the ‘Regent’ and the ‘Sibera’ varieties reached physiological ripeness on 30th September and 3rd October, respectively. On both harvest dates, in addition to soluble solids content, titratable acidity, and pH, the macronutrient content was also determined. In the phase of physiological ripeness, the ‘Sibera’ variety showed higher acidity (1.02 g · 100 mL−1) compared with the ‘Regent’ (0.87 g · 100 mL−1). A higher soluble solids content was found in the ‘Regent’ (20.4°Brix), and slightly lower in the ‘Sibera’ (18.1°Brix). The must of the ‘Regent’ had a higher pH (3.5). This variety also had higher macronutrient contents (K, Ca and Mg). Grapes harvested after 3 weeks (late harvest) showed higher values of sugars, pH, K, and P for both varieties. However, their titratable acidity content, and Ca and Mg contents decreased. Microscopic examination showed differences in mycelial growth of Botrytis cinerea on grape bunches for both of the varieties left for the late harvest. The mycelial growth promoted faster dehydration of the ‘Sibera’ berries. Spot chemical analyses of ‘Sibera’ berry peel performed using EDS (energy dispersive spectroscopy) showed the occurrence of elevated contents of potassium, phosphorus, sulphur, magnesium and silicon. Around skin cracks, sugar crystals as well as hard-to-identify microcrystals were formed containing potassium. On the surface of the ‘Regent’ berries, potassium, and traces of phosphorus, silicon, magnesium, sulphur and calcium were found.
Effect of no-tillage and tillage systems on melon (Cucumis melo L.) yield, nutrient uptake and microbial community structures in greenhouse soils
Authors: Zhang Jian , Wang Jiajia , Wang Pengcheng , Guo Tingting 
Published: October 2020

Abstract
No-tillage (UT) and tillage (TL) influence melon (Cucumis melo L.) production. However, the mechanism of improving the soil quality under UT in melon production is still unavailable. In this study, we attempted to explore the effects of UT and TL treatments on soil fertility and the microbial abundance and diversity in planting melon under greenhouse condition. Soil properties were determined and the bacterial v4-v5 16S rRNA and the fungal internal transcribed spacer gene were pyrosequenced by extracting greenhouse soil DNA. Results showed that the two treatments had different effects on nutrient uptake in melon plants under facility conditions. Additional nitrogen (N) was absorbed in the leaves and fruit in UT treatment. However, the N content in the UT treatment was kept as similar to that of the TL treatment. The phosphorus (P) contents in melon plant leaves and fruits in the UT treatment were higher than those in the TL treatment. High potassium (K) contents were observed in fruits and melon stem under the UT and TL treatments, respectively. Soil pH, organic matter and the available N influenced the bacterial and fungal distributions. The total N, total P and total K in melon plants were correlated with the bacterial and fungal groups in facility soils. The UT treatment had a substantial effect on the microbial diversity in soils planted with melon. Our study provided insights into the response of soil fertility and microbial structures to UT and TL treatments under greenhouse soils, which may aid in managing greenhouse soil quality.
National Lists of Scholarly Publication Channels: An Overview and Recommendations for Their Construction and Maintenance
Authors: Pölönen Janne , Guns Raf , Kulczycki Emanuel , Sivertsen Gunnar , Engels Tim C. E. 
Published: October 2020

Abstract

Purpose
This paper presents an overview of different kinds of lists of scholarly publication channels and of experiences related to the construction and maintenance of national lists supporting performance-based research funding systems. It also contributes with a set of recommendations for the construction and maintenance of national lists of journals and book publishers.

Design/methodology/approach
The study is based on analysis of previously published studies, policy papers, and reported experiences related to the construction and use of lists of scholarly publication channels.

Findings
Several countries have systems for research funding and/or evaluation, that involve the use of national lists of scholarly publication channels (mainly journals and publishers). Typically, such lists are selective (do not include all scholarly or non-scholarly channels) and differentiated (distinguish between channels of different levels and quality). At the same time, most lists are embedded in a system that encompasses multiple or all disciplines. This raises the question how such lists can be organized and maintained to ensure that all relevant disciplines and all types of research are adequately represented.

Research limitation
The conclusions and recommendations of the study are based on the authors’ interpretation of a complex and sometimes controversial process with many different stakeholders involved.

Practical implications
The recommendations and the related background information provided in this paper enable mutual learning that may feed into improvements in the construction and maintenance of national and other lists of scholarly publication channels in any geographical context. This may foster a development of responsible evaluation practices.

Originality/value
This paper presents the first general overview and typology of different kinds of publication channel lists, provides insights on expert-based versus metrics-based evaluation, and formulates a set of recommendations for the responsible construction and maintenance of publication channel lists.
Reliability Analysis of Alarm Signals Transmitting Systems Used to Monitoring Buildings
Authors: Jakubowski Krzysztof , Paś Jacek 
Published: October 2020

Abstract
The article presents a general method for identity the security risk factor for alarm signal transmission systems (ASTS), which are used in monitoring buildings and critical infrastructure. The security of the transmission of alarm signals to an alarm receiving center (ARC) is a property of the process, which is associated witch performing of tasks and functions, human factor, hardware and environment components. The process of transmitting alarm signals should be characterized by minimizing risk of external and internal interception/distortion or deformation of transmitted signals and in ideal case signals should be encrypted and integrity checked. Determining the security risk indicator for ASTS will allow optimal organization of electronic security systems.
Application of the LifeRoSE Method in Funcionality Evaluation of Road Safety Equipment
Authors: Gobis Anna , Jamroz Kazimierz , Jeliński Łukasz 
Published: October 2020

Abstract
The article presents a mathematical model of the life cycle estimation method of road safety equipment. Then the model was adjusted to estimate the life cycle costs of the chosen horizontal road marking. Using the LCC method, the functionality of the horizontal marking was evaluated in terms of efficiency, durability and economic effectiveness. The article also presents the impact of selected factors on the life cycle costs of the horizontal road marking.
Monitoring the Vector Error Between a Reference Station and a GNSS on-Board Receiver in the GBAS System in the Polish Air Transport
Authors: Kirschenstein Małgorzata , Krasuski Kamil , Goś Artur 
Published: October 2020

Abstract
The article presents research results concerning the determination of the vector error between a reference station and the GNSS on-board receiver in the GPS satellite measurements for GBAS system, taking into account the ZTD troposphere delay parameter. Based on the conducted studies, it was found that the highest value of the vector error between a reference station and the GNSS on-board receiver can exceed 0.18 m, for a distance of over 40 km and the ZTD value equalling to 2428.1 mm. The error results of vector measurement can be used in the RTK-OFT differential technique in the GBAS system.
Prototype of a Device Used for Dynamic Balancing Power Units of Unmanned Platforms
Authors: Ciszkowski Jarosław , Niszczota Paweł , Krogulec Przemysław 
Published: October 2020

Abstract
The paper presents a prototype of a device used for dynamic balancing of motors and power units utilized in the construction of unmanned electric aerial vehicles. The concept of research was described and a measuring system depiction was included. A three-dimensional model of a device functional prototype with its description was demonstrated. Additionally, research results of the system in the form of graphs were included.
Safety Analysis in Water Supply Systems
Authors: Tchórzewska-Cieślak Barbara , Boryczko Krzysztof 
Published: October 2020

Abstract
In the aspect of water management, the Water Directive regulates water management with the principle of sustainable development, in shaping, protecting, using and managing water resources. Analysis and assessment of the CWSS safety is a complex issue, including the analysis of potential threats, their effects and safety systems (barriers). The analysis is performed in terms of the water consumers health safety, threats of lack or deficit in water supply and threats to the environment. This process should include the analysis of the existing condition, possible potential threats and safety and repair procedures.
Simulator Verification of Cessna 172 RG Repeated Take-Off Runs in Extreme Temperature Conditions
Authors: Kalavsky Peter , Antosko Matej , Rozenberg Robert , Cekan Peter , Kelemen Miroslav , Kozuba Jarosław , Peter Kalavsky 
Published: October 2020

Abstract
The paper presents the results of the research of performance measurement of a selected aircraft type in the take-off phase under extreme temperature conditions. For this purpose, a flight simulator of the Cessna 172 RG aircraft from the ELITE Company was used. For the purpose of verifying the take-off run length, the article provides a measurement methodology that was developed using information obtained during experimental take-offs. The aim was to obtain a procedure that would allow for repeated take-off runs in the same conditions with the possibility of changing individual influencing factors. Considering the whole measurement chain, the article analyses the influencing factors and quantifies their impact on the uncertainty of the measurement result. The data obtained experimentally we compared with the data in the Flight Manual and at the end carried out the assessment of the impact of global warming on the take-off run of the Cessna 172 RG and generally on the safety of the take-off and on air transport..