bluuebunny/crossref_metadata_2025_split huggingface dataset:
The roles of circRFWD2 and circINO80 during NELL‐1‐induced osteogenesis
Authors: Huang Xinqi , Cen Xiao , Zhang Bo , Liao Yuwei , Zhao Zhenxing , Zhu Guanyin , Zhao Zhihe , Liu Jun 
Published: October 2019

AbstractBone defects caused heavy social and economic burdens worldwide. Nel‐like molecule, type 1 (NELL‐1) could enhance the osteogenesis and the repairment of bone defects, while the specific mechanism remains to be elucidated. Circular RNAs (circRNAs) have been found to play critical roles in the tissue development and serve as biomarkers for various diseases. However, it remains unclear that the expression patterns of circRNAs and the roles of them played in recombinant NELL‐1‐induced osteogenesis of human adipose‐derived stem cells (hASCs). In this study, we performed RNA‐sequencing to investigate the expression profiles of circRNAs in recombinant NELL‐1‐induced osteogenic differentiation and identified two key circRNAs, namely circRFWD2 and circINO80. These two circRNAs were confirmed to be up‐regulated during recombinant NELL‐1‐induced osteogenesis, and knockdown of them affected the positive effect of NELL‐1 on osteogenesis. CircRFWD2 and circINO80 could interact with hsa‐miR‐6817‐5p, which could inhibit the osteogenesis. Silencing hsa‐miR‐6817‐5p could partially reverse the negative effect of si‐circRFWD2 and si‐circINO80 on the osteogenesis. Therefore, circRFWD2 and circINO80 could regulate the expression of hsa‐miR‐6817‐5p and influence the recombinant NELL‐1‐induced osteogenic differentiation of hASCs. It opens a new window to better understanding the effects of NELL‐1 on the osteogenic differentiation of hASCs and provides potential molecular targets and novel methods for bone regeneration efficiently and safely.
Trajectories of care for children and adolescents with psychosocial problems: a 3‐year prospective cohort study
Authors: Verhage Vera , Jansen Danielle E.M.C. , Almansa Josue , Wunderink Charlotte , Grietens Hans , Reijneveld Sijmen A. 
Published: October 2019

BackgroundCare for children and adolescents with psychosocial problems is aimed at reducing problems. There may be a relationship between the intensity and duration of care provision and improvement of these outcomes, but evidence on this issue is lacking. We therefore examined the association between care trajectories based on duration and intensity of care for children, and the reduction in psychosocial problems after 3 years.MethodsWe obtained a cohort of all children entering psychosocial care in one region (n = 1,378), the TAKECARE cohort, and followed it for 3 years, with five assessment rounds. Retention in the final round was 85.8%. Psychosocial problems were measured using the parent report of the Total Difficulty Score of the Strength and Difficulties Questionnaire (SDQ‐TDS). We constructed trajectories for intensity of care using growth mixture modelling and assessed the association between duration and intensity of care trajectories and SDQ‐TDS after 3 years.ResultsAfter 3 months 60.6% of children and adolescents were receiving care, after 1 year 38.7% were receiving care and after 3 years 26.0%. Regarding intensity of care, three trajectories were found: one with minimal intensity during all 3 years, a second with initially medium intensity and strong reduction within 1 year, and a third with high intensity and a reduction after 1 year. Although the psychosocial problems of children and adolescents were reduced during the 3‐year period, the rate of decline was relatively less marked for children and adolescents with longer care trajectories.ConclusionOverall, children and adolescents with psychosocial problems who received care had improved outcomes at follow‐up. However, increased provision of care does not automatically lead to reduction of problems, and although overall psychosocial problems are reduced, a substantial subgroup has longer lasting problems.
Identification of a novel melatonin‐binding nuclear receptor: Vitamin D receptor
Authors: Fang Nan , Hu Chunyi , Sun Wenqi , Xu Ying , Gu Yeqi , Wu Le , Peng Qing , Reiter Russel J. , Liu Lifeng 
Published: October 2019

AbstractPrevious studies confirmed that melatonin regulates Runx2 expression but the mechanism is unclear. There is a direct interaction between Runx2 and the vitamin D receptor (VDR). Herein, we observed a direct interaction between melatonin and the VDR but not Runx2 using isothermal titration calorimetry. Furthermore, this direct binding was detected only in the C‐terminal ligand binding domain (LBD) of the VDR but not in the N‐terminal DNA‐binding domain (DBD) or the hinge region. Spectrophotometry indicated that melatonin and vitamin D3 (VD3) had similar uptake rates, but melatonin's uptake was significantly inhibited by VD3 until the concentration of melatonin was obviously higher than that of VD3 in a preosteoblastic cell line MC3T3‐E1. GST pull‐down and yeast two‐hybrid assay showed that the interactive smallest fragments were on the 319‐379 position of Runx2 and the N‐terminus 110‐amino acid DBD of the VDR. Electrophoretic mobility shift assay (EMSA) demonstrated that Runx2 facilitated the affinity between the VDR and its specific DNA substrate, which was further documented by a fluorescent EMSA assay where Cy3 labeled Runx2 co‐localized with the VDR‐DNA complex. Another fluorescent EMSA assay confirmed that the binding of the VDR to Runx2 was significantly enhanced with an increasing concentrations of the VDR, especially in the presence of melatonin; it was further documented using a co‐immunoprecipitation assay that this direct interaction was markedly enhanced by melatonin treatment in the MC3T3‐E1 cells. Thus, the VDR is a novel melatonin‐binding nuclear receptor, and melatonin indirectly regulates Runx2 when it directly binds to the LBD and the DBD of the VDR, respectively.
Vision‐based automated bridge component recognition with high‐level scene consistency
Authors: Narazaki Yasutaka , Hoskere Vedhus , Hoang Tu A. , Fujino Yozo , Sakurai Akito , Spencer Billie F. 
Published: October 2019

AbstractThis research investigates vision‐based automated bridge component recognition, which is critical for automating visual inspection of bridges during initial response after earthquakes. Semantic segmentation algorithms with up to 45 convolutional layers are applied to recognize bridge components from images of complex scenes. One of the challenges in such scenarios is to get the recognition results consistent with high‐level scene structure using limited amount of training data. To impose the high‐level scene consistency, this research combines 10‐class scene classification and 5‐class bridge component classification. Three approaches are investigated to combine scene classification results into bridge component classification: (a) naïve configuration, (b) parallel configuration, and (c) sequential configuration of classifiers. The proposed approaches, sequential configuration in particular, are demonstrated to be effective in recognizing bridge components in complex scenes, showing less than 1% of accuracy loss from the naïve/parallel configuration for bridge images, and less than 1% false positives for the nonbridge images.
A citizen science approach to long‐term monitoring of humpback whales (Megaptera novaeangliae) off Sydney, Australia
Authors: Pirotta Vanessa , Reynolds Wayne , Ross Geoffrey , Jonsen Ian , Grech Alana , Slip David , Harcourt Robert 
Published: October 2019

AbstractThe Cape Solander Whale Migration Study is a citizen science project that annually counts northward migrating humpback whales (Megaptera novaeangliae) off Cape Solander, Sydney, Australia. Dedicated observers have compiled a 20‐year data set (1997–2017) of shore‐based observations from Cape Solander's high vantage point. Using this long‐term data set collected by citizen scientists, we sought to estimate the humpback whale population trend as it continues to recover postexploitation. We estimated an exponential growth rate of 0.099 (95% CI = 0.079–0.119) using a generalized linear model, based on observer effort (number of observation days) and number of whales observed, equating to 10% per annum growth in sightings since 1997. We found that favorable weather conditions for spotting whales off Cape Solander consisted of winds <30 km/hr from a southerly through a north westerly direction. Incidental observations of other cetacean species included the endangered blue whale (Balaenoptera musculus) and data deficient species such as killer whales (Orcinus orca) and false killer whales (Pseudorca crassidens). Citizen science‐based studies can provide a cost‐effective approach to monitoring wildlife over the time necessary to detect change in a population. Information obtained from citizen science projects like this may help inform policy makers responsible for State and Federal protection of cetaceans in Australian waters and beyond.
PLAID syndrome: Characteristic presentation and a novel therapeutic option
Authors: Shea James , Huynh Thy , Milner Joshua , Chamlin Sarah 
Published: October 2019

AbstractPLCG2‐associated antibody deficiency and immune dysregulation (PLAID) is an autosomal dominant inherited disease caused by genomic deletion in PLCG2 and is characterized by cold urticaria, humoral immune deficiency, cutaneous granulomas, and autoimmune disease. The patient described in this case had a typical presentation for a PLAID phenocopy and experienced intense pruritus, a common complication of PLAID, starting in early childhood. After trialing H1 and H2 blockers with no improvement, oral glycopyrrolate was used with near resolution of the patient's symptoms. Given that the pruritus in PLAID is related to sweat‐induced evaporative cooling, practitioners who encounter this disease should consider glycopyrrolate in their management of PLAID‐associated pruritus.
Hangnails: Paste them back
Authors: Kaur Ishmeet , Jakhar Deepak , Misri Rachita 
Published: October 2019

AbstractHangnails are short torn down part of skin surrounding the nails. At times they are very painful. The usual treatment advised is cutting the excess skin with clippers or scissors. To provide an instant relief to the patients, we describe a simpler and effective way to use a surgical glue to paste them back in their original position.
The labor market effects of the China Syndrome: Evidence from South Korean manufacturing
Authors: Choi Jaerim , Xu Mingzhi 
Published: October 2019

AbstractWe evaluate the direct impact of China trade shock on the Korean labour market following the approach of Acemoglu, Autor, Dorn, Hanson, and Price (Journal of Labor Economics, 2016, 34, S1). Using firm‐ and industry‐level data for the period 1993–2013, our direct estimates imply that the net employment effect of the China shock in the manufacturing sector is the creation of 0.52 million jobs. The positive impact is mostly driven by China's rising demand for intermediate inputs and capital goods from Korea to support its export expansion to the global economy. The import‐competition channel plays a negligible role in manufacturing employment because it creates temporary jobs that merely compensate for the loss in permanent jobs. By contrast, over the same period, the average wage declined by 2.4%, and income inequality, measured as the gap between the high‐ and low‐income quantile, grew substantially in manufacturing. In addition, we find that the direct effect of China shock lowers labour market concentration by shifting workers from big firms to small‐ and medium‐sized firms.
Time-Dependent Reliability Model of Structures Under Different Stochastic Loads
Authors: Qian Hua-Ming , Huang Tudi , Huang Hong-Zhong 
Published: October 2019

AbstractThis paper proposes the time-dependent reliability model of structures under different stochastic loads based on the stress–strength interference (SSI) model and probabilistic statistical theory. First, the different stochastic loads mean that the loads are discrete, and that one applied load is from random variable and the other applied load may be from another random variable, and the cumulative effect and correlation are considered when they are applied for multiple times. Second, two different time-dependent reliability models, with and without considering strength degradation, are established in this paper. Meanwhile, the relationship between the discrete load frequency and time is described using Poisson process, and the time-dependent reliability model under continuous time is also provided. Finally, the proposed time-dependent reliability model of structures is validated with the Monte Carlo simulation (MCS), and the effectiveness of the proposed method is testified.
Difference of Cyclooxygenase-2 (COX2) Expression in Endometrium Between Patients with Polycystic Ovary Syndrome (PCOS) and Fertile Women
Authors: Budihastuti Uki Retno , Melinawati Eriana , Sulistyowati Sri , Laqief Abdurrahman , Dekawan M. Firdinan 
Published: October 2019

Objective: The study was conducted to determine whether there were differences in COX-2 expression in endometrial women with PCOS compared to fertile women. Methods: This study is a case-control study investigating the relationship between exposure (research factors) and disease, by comparing case group and control group based on their exposure status. The samples of this study were infertile polycystic ovary syndrome patients who were treated at the Sekar Fertility Clinic in Dr. Moewardi Hospital Surakarta, and fertile women seeking treatment at Dr. Moewardi Hospital Surakarta. The number of samples were 60 subjects consisting of 30 PCOS patients and 30 fertile women. The expression of COX-2 in endometrial biopsy LH + 5 until LH + 10 which meet the inclusion with Rotterdam criteria and exclusion criteria was checked by immunohistochemistry. The data was analyzed using the Mann-Whitney test. Results: The mean COX-2 expression in PCOS (10.83 ± 5.35) in fertile women (37.00 ± 7.76), p = 0.005. Regression test of COX-2 by adjusting external variables (occupation, age, education, menstrual disorders, familial history, menstrual cycle, menarche, obesity, contraception history) shows also higher expression in PCOS patient with OR = -7.063; CI = 0.462–108.066; p = 0.160. Conclusion: COX-2 expression in endometrium of women with PCOS is lower than it is in fertile women.
Effect of Sulfated Polysaccharide from Undaria pinnatifida (SPUP) on Proliferation, Migration, and Apoptosis of Human Prostatic Cancer
Authors: Xu Xiaolin , Zhu Xin , Lu Wenglong , He Yandong , Wang Yihan , Liu Feng 
Published: October 2019

Objective. To observe the effect of sulfated polysaccharide from Undaria pinnatifida (SPUP) on proliferation, migration, and apoptosis of human prostatic cancer. Methods. DU145 human prostate cancer cells were cultured in vitro, and the proliferation activity both in the control group and the SPUP treatment groups (25, 50, 100, 200 μg/ml) was measured by CCK-8 assay. The wound healing assay was conducted to detect the cell migration. Cell apoptosis was measured by flow cytometry. The protein and mRNA expressions of matrix metalloproteinase-9 (MMP-9) and apoptosis-related factor Bax were detected by qRT-PCR and Western blot. The expressions of cleaved caspase-3 and cleaved caspase-9 were also determined by Western blot. Results. (1) CCK-8 results showed that the proliferative activity of DU145 cells was significantly decreased with the increase of SPUP treatment concentration (P<0.05) in a dose-dependent manner and that the inhibitory effect of SPUP was most significant at 72 h (P<0.05) as compared with the control group; (2) the migration rate of SPUP-treated cells was significantly decreased (P<0.05) as compared with the control group. And the results of qRT-PCR and Western blot assays showed that SPUP inhibited the expression of MMP-9 in DU145 cells; (3) compared with the control group, the SPUP-treated groups had increased apoptosis of the cells. The expressions of apoptosis-related factors cleaved caspase-3, cleaved caspase-9, and Bax were upregulated (P<0.05), and the mRNA expression of Bax was increased (P<0.05). Conclusion. SPUP showed an antitumor activity in prostatic cancer, and the underlying mechanism may be pertaining to inhibition of migration, proliferation, and induction of apoptosis of cancer cells.
Phase I Clinical Trial of Selinexor in Combination with Daunorubicin and Cytarabine in Previously Untreated Poor-Risk Acute Myeloid Leukemia
Authors: Sweet Kendra , Komrokji Rami , Padron Eric , Cubitt Christopher L. , Turner Joel G. , Zhou Junmin , List Alan F. , Sallman David A. , Dawson Jana L. , Sullivan Daniel M. , Chavez Julio , Shah Bijal D. , Lancet Jeffrey E. 
Published: October 2019

Abstract

Purpose:
Induction chemotherapy results in complete remission (CR) rates of 20% to 50% among patients with poor-risk AML. Selinexor is an oral selective inhibitor of nuclear export with promising single-agent activity. By inhibiting the primary export protein, XPO1, selinexor localizes and activates tumor suppressor proteins in the nucleus and inhibits DNA damage repair, rationalizing combination with DNA-damaging agents.

Patients and Methods:
This was a single-arm phase I clinical trial of selinexor combined with cytarabine and daunorubicin (7+3). Dose escalation was selinexor alone (3+3) with an expansion at the MTD. Cohorts 1 and 2 received 60 and 80 mg orally, respectively, twice weekly during induction. Consolidation cycles (≤ 2) with selinexor at induction dose plus 5+2 were allowed for patients who achieved CR. MTD and recommended phase II dose of selinexor were the primary endpoints.

Results:
Twenty-one patients with poor-risk AML were enrolled. All 21 patients were included in the safety evaluations and survival analyses (4 in each of 2 cohorts; 13 in the expansion); 8 (53%) of the 19 patients evaluable for response achieved CR/CRi. MTD was not reached. Selinexor 80 mg (orally, twice weekly) was used in the expansion phase. The most common grade 3/4 nonhematologic treatment-emergent adverse events were febrile neutropenia (67%), diarrhea (29%), hyponatremia (29%), and sepsis (14%). At median follow-up (28.9 months), 38% of patients were alive. Median overall survival was 10.3 months.

Conclusions:
Selinexor plus 7+3 is a safe regimen for patients with newly diagnosed poor-risk AML and warrants further investigation in a larger clinical trial.
El papel del movimiento Jaranero en la salvaguarda del fandango Jarocho (México)
Authors: Sonderegger Melba , Lessa Giane Da Silva Mariano 
Published: October 2019

El fandango jarocho es un evento festivo comunitario con música y baile, producto del mestizaje, cuya tradición hunde sus raíces desde la época colonial. Durante la primera mitad del siglo XX, el fandango jarocho atravesó un punto crítico para la continuación de su práctica, por lo que, a partir de la década de los 80, surge un colectivo interesado en su investigación, revitalización y difusión, denominado Movimiento jaranero. En estas páginas se explicita el proceso de salvaguarda del fandango jarocho iniciado por el Movimiento jaranero, entendido como una articulación socio-estatal, que buscó garantizar una generación de relevo y la pervivencia del fandango. Como resultado de la tensión entre pasado y presente, actualmente el fandango y el sonjarocho – el género musical que le hace parte – se manifiestan en diversas esferas y con variacionesque alcanzan incluso la fusión con otros géneros musicales.
Evolução estrutural dos Granitoides Arroio Divisa durante o movimento transcorrente da Zona de Cisalhamento Quitéria-Serra do Erval, Rio Grande do Sul
Authors: Schnorr Evelin Roberta , Bitencourt Maria De Fátima 
Published: October 2019

Os Granitoides Arroio Divisa (GAD) compreendem uma associação de rochas predominantemente granodioríticas, com termos dioríticos e tonalíticos em menor expressão. Apresentam textura heterogranular média a grossa e são sempre foliadas. Suas estruturas sugerem posicionamento em zona de cisalhamento dúctil correlacionada ao Cinturão de Cisalhamento Sul-brasileiro (CCSb), de idade neoproterozoica. Essas rochas registram cristalização com concomitante história deformacional sob condições de temperatura decrescente, evidenciada pela sobreposição concordante de estruturas magmáticas por estruturas de deformação de estado sólido em temperatura decrescente. A distribuição da deformação se dá de forma heterogênea ao longo da intrusão, gerando-se zonas de baixa e alta deformação inicialmente magmáticas e progredindo para o estado sólido. Nas porções internas do corpo intrusivo predominam estruturas de fluxo em que o alinhamento preferencial de forma é dominante, e a deformação interna dos marcadores é pouco desenvolvida. Em direção à borda norte da intrusão, a morfologia dessas estruturas progride por aumento na intensidade da deformação, com a geração de uma foliação fortemente milonítica paralela à trama magmática. Feições microestruturais de alta temperatura incluem o desenvolvimento de subgrãos em padrão tabuleiro de xadrez no quartzo, “e a geração de subgrãos grandes em cristais de K-feldspato e plagioclásio, que são compatíveis com temperaturas da fácies anfibolito superior e com a temperatura solidus de composições granítcas. As feições microestruturais de baixa temperatura consistem na recristalização dos cristais de quartzo por bulging, na neoformação de grãos finos ao redor dos cristais de feldspatos e no desenvolvimento de pertitas em chamas no K-feldspato, compatíveis com temperaturas da fácies xisto verde, bem abaixo da solidus. Portanto, as microestruturas de alta temperatura estariam associadas aos estágios iniciais de resfriamento
A20 in Myeloid Cells Protects Against Hypertension by Inhibiting Dendritic Cell-Mediated T-Cell Activation
Authors: Lu Xiaohan , Rudemiller Nathan P. , Wen Yi , Ren Jiafa , Hammer Gianna E. , Griffiths Robert , Privratsky Jamie R. , Yang Bo , Sparks Matthew A. , Crowley Steven D. 
Published: October 2019

Rationale:
The ubiquitin-editing protein A20 in dendritic cells (DCs) suppresses NF-κB (nuclear factor-κB) signaling and constrains DC-mediated T-cell stimulation, but the role of A20 in modulating the hypertensive response requires elucidation.

Objective:
Here, we tested the hypothesis that A20 in CD11c-expressing myeloid cells mitigates Ang II (angiotensin II)–induced hypertension by limiting renal T-cell activation.

Methods and Results:

Mice with heterozygous deletion of A20 in CD11c-expressing myeloid cells (DC ACT[
Cd11c-Cre
+
A20

flox/wt

]) have spontaneous DC activation but have normal baseline blood pressures. In response to low-dose chronic Ang II infusion, DC ACT mice compared with WT (wild type) controls had an exaggerated hypertensive response and augmented proportions of CD62L
lo
CD44
hi
effector memory T lymphocytes in the kidney lymph node. After 10 days of Ang II, DC ACT kidneys had increased numbers of memory effector CD8
+
, but not CD4
+
T cells, compared with WTs. Moreover, the expressions of TNF-α (tumor necrosis factor-α) and IFN-γ (interferon-γ) were upregulated in the DC ACT renal CD8
+
T cells but not CD4
+
T cells. Saline challenge testing revealed enhanced renal fluid retention in the DC ACT mice. DC ACT kidneys showed augmented protein expression of γ-epithelial sodium channel and NHE3 (sodium-hydrogen antiporter 3). DC ACT mice also had greater reductions in renal blood flow following acute injections with Ang II and enhanced oxidant stress in the vasculature as evidenced by higher circulating levels of malondialdehyde compared with WT controls. To directly test whether enhanced T-cell activation in the DC ACT cohort was responsible for their exaggerated hypertensive response, we chronically infused Ang II into lymphocyte-deficient DC ACT Rag1 (recombination activating protein 1)-deficient (
Rag1
−/−
) mice and WT (
Cd11c
-
Cre
−
A20

flox/wt

) Rag1
−/−
controls. The difference in blood pressure elevation accruing from DC activation was abrogated on the Rag1
−/−
strain.

Conclusions:
Following stimulation of the renin-angiotensin system, A20 suppresses DC activation and thereby mitigates T-cell–dependent blood pressure elevation.