bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tubulin‐Based Microtentacles Aid in Heterotypic Clustering of Neutrophil‐Differentiated HL‐60 Cells and Breast Tumor Cells
Authors: Ju Julia A. , Thompson Keyata N. , Annis David A. , Mull Makenzy L. , Gilchrist Darin E. , Moriarty Aidan , Chang Katarina T. , Stemberger Megan B. , Noto Michael J. , Vitolo Michele I. , Martin Stuart S. 
Published: December 2024

AbstractCirculating tumor cells (CTCs) travel through the vasculature to seed secondary sites and serve as direct precursors of metastatic outgrowth for many solid tumors. Heterotypic cell clusters form between CTCs and white blood cells (WBCs) and recent studies report that a majority of these WBCs are neutrophils in patient and mouse models. The lab discovered that CTCs produce tubulin‐based protrusions, microtentacles (McTNs), which promote reattachment, retention in distant sites during metastasis and formation of tumor cell clusters. Neutrophil‐CTC clusters help CTCs survive the harsh vascular environment to promote successful metastasis, however, the specific mechanism of this interaction is not fully understood. Utilizing TetherChip technology, it is found that primary and differentiated neutrophils produce McTNs composed of detyrosinated and acetylated α‐tubulin and vimentin. Neutrophil McTNs aid in cluster formation, migration, and reattachment, which are suppressed with the tubulin‐depolymerizing agent, Vinorelbine. Co‐culturing differentiated neutrophils and tumor cells formed heterotypic clusters that enhanced migration. CTC‐neutrophil clusters have higher metastatic efficiency, and by demonstrating that neutrophils form McTNs, a new possible mechanism for how neutrophils interact with tumor cells is revealed. These findings further support the idea that developing cluster‐disrupting therapies can provide a new targeted strategy to reduce the metastatic potential of cancer cells.
Alzheimer's disease‐associated CD83(+) microglia are linked with increased immunoglobulin G4 and human cytomegalovirus in the gut, vagal nerve, and brain
Authors: Readhead Benjamin P. , Mastroeni Diego F. , Wang Qi , Sierra Maria A. , de Ávila Camila , Jimoh Tajudeen O. , Haure‐Mirande Jean‐Vianney , Atanasoff Kristina E. , Nolz Jennifer , Suazo Crystal , Barton Nathaniel J. , Orszulak Adrian R. , Chigas Samantha M. , Tran Khanh , Mirza Anne , Ryon Krista , Proszynski Jacqueline , Najjar Deena , Dudley Joel T. , Liu Sean T. H. , Gandy Sam , Ehrlich Michelle E. , Alsop Eric , Antone Jerry , Reiman Rebecca , Funk Cory , Best Rebecca L. , Jhatro Michael , Kamath Kathy , Shon John , Kowalik Timothy F. , Bennett David A. , Liang Winnie S. , Serrano Geidy E. , Beach Thomas G. , Van Keuren‐Jensen Kendall , Mason Christopher E. , Chan Yingleong , Lim Elaine T. , Tortorella Domenico , Reiman Eric M. 
Published: December 2024

AbstractINTRODUCTIONWhile there may be microbial contributions to Alzheimer's disease (AD), findings have been inconclusive. We recently reported an AD‐associated CD83(+) microglia subtype associated with increased immunoglobulin G4 (IgG4) in the transverse colon (TC).METHODSWe used immunohistochemistry (IHC), IgG4 repertoire profiling, and brain organoid experiments to explore this association.RESULTSCD83(+) microglia in the superior frontal gyrus (SFG) are associated with elevated IgG4 and human cytomegalovirus (HCMV) in the TC, anti‐HCMV IgG4 in cerebrospinal fluid, and both HCMV and IgG4 in the SFG and vagal nerve. This association was replicated in an independent AD cohort. HCMV‐infected cerebral organoids showed accelerated AD pathophysiological features (Aβ42 and pTau‐212) and neuronal death.DISCUSSIONFindings indicate complex, cross‐tissue interactions between HCMV and the adaptive immune response associated with CD83(+) microglia in persons with AD. This may indicate an opportunity for antiviral therapy in persons with AD and biomarker evidence of HCMV, IgG4, or CD83(+) microglia.Highlights
Cross‐tissue interaction between HCMV and the adaptive immune response in a subset of persons with AD.
Presence of CD83(+) microglial associated with IgG4 and HCMV in the gut.
CD83(+) microglia are also associated presence of HCMV and IgG4 in the cortex and vagal nerve.
Replication of key association in an independent cohort of AD subjects.
HCMV infection of cerebral organoids accelerates the production of AD neuropathological features.
Degradable Polyvinyl Alcohol Liquid Mulch Film Synergistically Reinforced by Cellulose and Lignin
Authors: Wang Zheng , Yang Zhiwei , Qiao Pengju , Fang Jing , Wu Xingjiang , Yang Zhensheng , Li Hao 
Published: December 2024

ABSTRACTDegradable polyvinyl alcohol (PVA) liquid mulch film is an effective alternative to traditional petroleum‐based mulch. However, PVA has problems such as poor water resistance and high cost in practical applications. In this work, a liquid mulch film PCQ was prepared by simply blending quaternized lignin (QAL), carboxymethyl cellulose (CMC), and PVA. PCQ was characterized and analyzed through FT‐IR, SEM, and other tests. The effects of QAL and CMC on the water resistance, barrier properties, water retention, soil‐fixing properties, and degradation properties of PCQ were investigated. The results showed that the addition of QAL and CMC significantly enhanced the water resistance of the liquid mulch film and provided excellent UV shielding (90%) and water vapor shielding performance (249.3 g m−2 d−1). In addition, PCQ effectively immobilized topsoil, retained soil moisture, and improved seed germination. This study provides a new strategy for preparing environmentally friendly, low‐cost, and degradable liquid mulch film.
A simple method for improving generalizability in behavioral science: Scope Testing with AI‐Generated Stimuli (STAGS)
Authors: Tomaino Geoff , Mazar Asaf , Carmon Ziv , Wertenbroch Klaus 
Published: December 2024

AbstractBehavioral research typically tests hypotheses in a limited set of researcher‐selected contexts. This approach can reveal whether an effect can occur (possibility) but does not indicate whether it holds in other contexts (generalizability). We present Scope Testing with AI‐Generated Stimuli (STAGS), a simple approach that uses Generative AI (GenAI) to test predictions across a range, or scope, of stimuli. By assessing whether a prediction holds across this range, STAGS sheds light on the generalizability of the effect. In addition, outsourcing stimulus generation to GenAI makes transparent the otherwise opaque process of stimulus selection, requiring researchers to articulate the scope of stimuli to which their hypothesis applies. We illustrate STAGS in an experiment, showing that specifying the population from which stimuli are sampled can help researchers understand the scope of the effect they are studying. We discuss the benefits and limitations of this approach and propose directions for future exploration.
Long‐term follow‐up of intradetrusor botulinum toxin utilisation: A comparison of patients with multiple sclerosis and idiopathic overactive bladder
Authors: Chui William , Kealey Joshua , Yao Henry H. , Chan Garson , Bazo Alvaro , Parkinson Richard , O'Connell Helen E. , Gani Johan 
Published: December 2024

AbstractObjectivesTo assess long‐term outcomes (up to 10 cycles) of repeated intradetrusor botulinum toxin (BoNT) utilisation in multiple sclerosis (MS) and idiopathic overactive bladder (OAB).Materials and MethodsThis is a retrospective, international, multi‐centre, observational cohort study of patients diagnosed with MS and neurogenic OAB and treated with intradetrusor BoNT between January 2005 and January 2020 (just prior to COVID‐19 interruption). Dose, efficacy, duration of effect, International Consultation on Incontinence Questionnaire Overactive Bladder (ICIQ‐OAB) and International Consultation on Incontinence Questionnaire Urinary Incontinence (ICIQ‐UI) measures and complication rates were recorded. Comparisons were made to those with idiopathic OAB.ResultsSeven hundred and ninety‐two patients received intradetrusor BoNT treatment (104 had MS with neurogenic OAB and 688 had idiopathic OAB). Patients with MS were more likely to receive higher doses of BoNT in all cycles. Self‐reported efficacy ranged from 85.7 to 100% (MS) and 87.2 to 100% (idiopathic) with MS patients reporting increased efficacy in cycles 1–3 comparatively (p < 0.05). Duration of effect ranged from 4.5 to 9 months with a reduction in the MS cohort between cycles 1 and 10 (median 8 months vs. 5 months, p = 0.0156).ConclusionPatients with MS and neurogenic OAB have a good therapeutic effect from intradetrusor BoNT that is sustained over at least 10 cycles with significant reduction in the duration of action with subsequent cycles.
Otocephaly Diagnosed Prenatally: A Case Study of a Rare Congenital Defect
Authors: Konukcu Birsen 
Published: December 2024

ABSTRACTBackgroundAgnathia‐otocephaly complex (AOC) is a rare and complex craniofacial malformation characterized by mandibular hypoplasia or agnathia, auricular fusion, microstomia with oroglossal hypoplasia or aglossia. It has a very bad prognosis and can arise alone or in conjunction with heart defects and forebrain abnormalities.Case ReportA standard second‐trimester ultrasound exam was recommended for, a 23‐year‐old primigravida woman who was at 22 weeks gestation. The oral fissure was “pinhole‐shaped,” the mouth was incredibly small, and the usual lower jaw and lower lip had vanished from the S‐curve. On the front of the neck were the two ears. Amniocentesis revealed a 46,XY normal karyotype. The family opted for a medically assisted termination. At 23 weeks, the pregnancy was ended by vaginal delivery. Observation of the specimen revealed that the ear placements were remarkably low, and the specimen's observation revealed that the two earlobes were joined at the front of the neck.ConclusionIdentifying the development of the mandible and locating auricles during prenatal ultrasound diagnosis was particularly challenging could be a better way to phrase this like in our recent example, the combination of two‐dimensional and three‐dimensional ultrasound was able to confirm the prenatal diagnosis of AOC.
Effect of Additives With Phenyl and Acid Anhydride Functional Groups on the Wide Temperature Operation Performance of LiNi0.8Co0.1Mn0.1O2||SiO/Graphite Pouch Cells
Authors: Wang Chengyun , Chen Jin , Feng Yaowei , Deng Xiuqin , Pang Xiaoxian , Zou Hanbo , Yang Wei , Chen Shengzhou , Xu Xijun 
Published: December 2024

ABSTRACTHigh‐nickel LiNi0.8Co0.1Mn0.1O2 (NCM811) cathode paired with silicon‐based graphite (SiO/Gr) is pivotal for enhancing the energy density of lithium‐ion batteries (LIBs). However, the high reactivity of NCM811 with the electrolyte and the volumetric expansion issues associated with SiO/Gr pose significant challenges to their practical application. To settle these issues, we explore the impact of additives with phenyl and acid anhydride moieties on the performance of NCM811‖SiO/Gr pouch cells across a wide temperature range of −20°C~60°C. Acid anhydride additives are capable of diminishing the internal resistance in NCM811‖SiO/Gr pouch cells, as well as curbing gas evolution and thickness increase during the operational phase. Notably, the batteries enriched with citraconic anhydride (CAn) and succinic anhydride (SAn) additives after 120 cycles at 45°C demonstrated enhanced capacity retention from 83.2% to 88.1% and 85.5%, respectively. Intriguingly, the inclusion of phenyl‐containing additives in the electrolyte was found to be advantageous for NCM811‖SiO/Gr pouch cells' low‐temperature performance. Furthermore, neither type of functional group significantly enhanced performance at room conditions. Consequently, the combination of additives is necessary to fulfill the stringent requirements of LIBs for extreme environment applications. This work guides designing composite electrolytes for high energy density wide temperature operation LIBs.
Research Progress on the Application of MOF Materials in Lithium‐Ion Batteries
Authors: Zhang Xiao , Ding Yanhuai 
Published: December 2024

ABSTRACTLithium‐ion batteries (LIBs) have established themselves as the preferred power sources for both pure electric and hybrid vehicles, attributable to their exceptional characteristics, including prolonged cycle life, elevated energy density, and minimal self‐discharge rates. Metal‐organic frameworks (MOFs), as innovative functional molecular crystal materials, exhibit promising application prospects in LIBs. This paper provides a comprehensive overview of the latest advancements in the synthesis techniques and structural modulation of MOFs and their derivative materials. It particularly emphasizes a thorough exploration of the utilization of MOFs and their derivatives in the anode, cathode, and separators of LIBs. Additionally, this paper delves into the current obstacles encountered by MOFs in LIB applications and offers insights into their potential future development.
Prognostic Implications of the Number of Lymph Node Metastases in Oral Tongue Squamous Cell Carcinoma: A Population Study of the SEER Database and an Institutional Registry
Authors: Huang Wenjie , Zhang Yu , Li Hao , Liang Zhiying , Zhou Shumin , Pan Jie , Xie Hui , Luo Chao , Li Shuqi , Ruan Guangying , Ai Fei , Chen Yanfeng 
Published: December 2024

ABSTRACTBackgroundTo investigate the impact of the number of positive lymph nodes (PLNs) on long‐term survival and pathological nodal stage in patients with oral tongue squamous cell carcinoma (OTSCC).Materials and MethodsNewly diagnosed and nonmetastatic adult patients with OTSCC who underwent curative resection were identified between January 2010 and December 2020. External validation was performed via the SEER registry. Multivariate Cox proportional hazards model was employed to calculate hazard ratios (HRs) and 95% confidence intervals (CIs) of pathological nodal features. Propensity score matching (PSM) was used to assess effect of adjuvant chemoradiotherapy (ACRT).ResultsAmong 518 curative‐intent OTSCC patients, the number of PLNs independently predicted overall survival (OS), surpassing other pathological nodal features, including extranodal extension, laterality, and lower neck involvement. Patients with 1 or 2 PLNs had comparable worse OS than those with no PLN (median OS of 1 PLN vs. 2 PLNs vs. 0 PLN: 35.1 vs. 30.5 vs. 40.2 months), but better than those with ≥ 3 PLNs (median OS of 1–2 PLNs vs. 3 PLNs: 32.1 vs. 19.0 months). A proposed nodal category with 0, 1–2 PLNs, and ≥ 3 PLNs exhibited increasingly worse OS (HR of 1–2 PLNs and ≥ 3 PLNs vs. 0 PLN: 2.98 [95% CI: 1.89–4.71], p < 0.001; 5.47 [95% CI: 3.33–9], p < 0.001; respectively) and showed improved prediction power versus current pN staging (C‐index: 0.717 vs. 0.713, p < 0.001). PSM analysis revealed that ACRT benefited patients with advanced nodal disease (≥ 3 PLNs) and improved OS. These findings were validated in SEER registry.ConclusionThe number of PLNs is a better predictor of overall tumor burden for OTSCC and could be a more accurate metric for survival estimation, which should be considered in future simplified pathological nodal staging for better risk stratification and decision‐making in subsites of the oral cavity.
What Do I Do if the Valve Don't Pass? Use a Snare Catheter!
Authors: Berger Simon , Combaret Nicolas , Souteyrand Geraud 
Published: December 2024

ABSTRACTWe report a case of a complex transcatheter aortic valve implantation (TAVI) complicated by severe calcifications, which prevented the delivery system from advancing through the aortic valve. To address this challenge, we employed an innovative solution using a Snare catheter. This approach enabled stabilization and guidance of the delivery system, facilitating the crossing of the calcified obstruction and the successful completion of the procedure. This case highlights the utility of the Snare catheter as a complementary technique to overcome anatomical challenges in complex TAVI procedures.
Class III Correction With Reverse Twin Block—A Case Report
Authors: Pandey Sushant , Gyawali Rajesh , Pokharel Prabhat Ranjan , Chaudhary Avinash , Sangroula Samikshya 
Published: December 2024

ABSTRACTThe orthodontic management of patients with Class III malocclusion poses numerous treatment challenges. Various removable, fixed, orthopedic, and myofunctional appliances have been recommended for its correction. The Reverse Twin Block is a removable appliance which has been used for the early management of such cases. A 10‐year‐old female with no significant medical and family history presented with the chief complaint of forward placement of lower front teeth on biting. Extraoral examination revealed a mesoprosopic face, a prominent lower lip, and a shallow mentolabial sulcus. Facial profile was straight at rest and concave at maximum intercuspation. Intraoral examination revealed a Class III incisor relationship with premature contact between 21 and 31, causing a forward path of closure of the mandible. The patient had a reverse overjet of 2 mm, an overbite of 5 mm, and a bilateral Super‐class I molar relationship at maximum intercuspation. Lateral cephalogram analysis revealed a mild skeletal class III denture base (ANB = 0°, Wits = −4 mm) due to a prognathic mandible (SNB = 82°). The treatment objectives included elimination of the premature contact, establishment of a positive overjet, and improvement of the facial profile. A Reverse Twin Block appliance was planned and prescribed for full‐time wear for 8 months. At the end of treatment, the patient had a Class I incisor relationship, straight facial profile, Class I molar relationship on the left side, and Super‐class I molar relationship on the right side. The Reverse Twin Block is a simple and well‐tolerated appliance that can effectively correct developing Class III malocclusion in carefully selected cases.
A Comprehensive Stereology Method for Quantitative Evaluation of Neuronal Injury, Neurodegeneration, and Neurogenesis in Brain Disorders
Authors: Reddy Doodipala Samba , Zhu Neo , Challa Trisha , Gajjela Sai , Desai Hetvi , Ramakrishnan Sreevidhya , Wu Xin 
Published: December 2024

AbstractNeuronal injury, neurodegeneration, and neuroanatomical changes are key pathological features of various neurological disorders, including epilepsy, stroke, traumatic brain injury, Parkinson's disease, autism, and Alzheimer's disease. Accurate quantification of neurons and interneurons in different brain regions is critical for understanding the progression of neurodegenerative disorders in animal models. Traditional scoring methods are often superficial, biased, and unreliable for evaluating neuropathology. Stereology, a quantitative tool that uses 3‐dimensional visualization of cells, provides a robust protocol for evaluating neuronal injury and neurodegeneration. This article presents a comprehensive and optimized stereology protocol for unbiased quantification of neuronal injury, neurodegeneration, and neurogenesis in rat and mouse models. This protocol involves precise counting of injured neurons, surviving neurons, and interneurons through immunohistochemical processing of brain sections for NeuN(+) principal neurons, parvalbumin (PV+) interneurons, doublecortin (DCX+) newborn neurons, and Fluoro‐Jade B (FJB+)‐stained injured cells. Predefined hippocampal and amygdala regions were identified and analyzed using a Visiopharm stereology software‐driven compound microscope. Cell density and absolute cell numbers were determined using the optical fractionation method. Our stereology protocol accurately estimated 1.5 million total NeuN(+) principal neurons and 0.05 million PV(+) interneurons in the rat hippocampus, as well as 1.2 million total principal neurons and 0.025 million interneurons in the mouse hippocampus. FJB(+) counting provided a quantitative index of damaged neurons, and the stereology of DCX(+) neurons demonstrated the extent of neurogenesis. Overall, this stereology protocol enables precise, accurate, and unbiased counting of total neurons in any brain region. This offers a reliable quantitative tool for studying neuronal injury and protection in various models of acute brain injury, neurotoxicity, and chronic neurological disorders. © 2024 Wiley Periodicals LLC.Basic Protocol 1: Stereological quantification of principal neurons, interneurons, and immature neurons in the hippocampus in rat brain sectionsBasic Protocol 2: Stereological quantification of principal neurons, interneurons, and immature neurons in the hippocampus in mouse brain sectionsBasic Protocol 3: Stereological quantification of injured or necrotized cells stained with Fluoro‐Jade B in the hippocampus and amygdala in ratsBasic Protocol 4: Stereological quantification of injured or necrotized cells stained with Fluoro‐Jade B in the hippocampus and amygdala regions in miceBasic Protocol 5: Brain fixation and histology processingBasic Protocol 6: Immunochemistry of principal neurons, interneurons, and newborn neuronsBasic Protocol 7: Fluoro‐Jade B staining of injured neurons
Development of a Microphysiological Cartilage‐on‐Chip Platform for Dynamic Biomechanical Stimulation of Three‐Dimensional Encapsulated Chondrocytes in Agarose Hydrogels
Authors: Peitso Valtteri , Sarmadian Zahra , Henriques João , Lauwers Elsa , Paggi Carlo Alberto , Mobasheri Ali 
Published: December 2024

AbstractOsteoarthritis (OA) is one of the most prevalent joint diseases globally, characterized by the progressive breakdown of articular cartilage, resulting in chronic pain, stiffness, and loss of joint function. Despite its significant socioeconomic impact, therapeutic options remain limited, largely due to an incomplete understanding of the molecular mechanisms driving cartilage degradation and OA pathogenesis. Recent advances in in vitro modeling have revolutionized joint tissue research, transitioning from simplistic two‐dimensional cell cultures to sophisticated three‐dimensional (3D) constructs that more accurately mimic the physiological microenvironment of native cartilage. Over the last decade, organ‐on‐chip technologies have emerged as transformative tools in tissue engineering, offering microphysiological platforms with precise control over biomechanical and biochemical stimuli. These platforms are providing novel insights into tissue responses and disease progression and are increasingly integrated into the early stages of drug screening and development. In this article, we present a detailed experimental protocol for constructing a cartilage‐on‐chip system capable of delivering controlled dynamic biomechanical stimulation to 3D‐encapsulated chondrocytes in an agarose hydrogel matrix. Our protocol, optimized for both bovine and human chondrocytes, begins with Basic Protocol 1, detailing the preparation and injection of cell‐laden hydrogels into the microdevice. Basic Protocol 2 describes the application of dynamic mechanical loading using a calibrated pressurized pump. Finally, Basic Protocols 3 and 4 focus on the retrieval of the hydrogel and RNA extraction for downstream molecular analyses. This platform represents a critical advancement for in vitro studies of cartilage biology, enabling more precise modeling of OA pathophysiology and evaluation of experimental therapeutics. © 2024 The Author(s). Current Protocols published by Wiley Periodicals LLC.Basic Protocol 1: Cartilage‐on‐chip injectionBasic Protocol 2: Cartilage‐on‐chip actuationBasic Protocol 3: Cartilage‐on‐chip agarose hydrogel removalBasic Protocol 4: Preparation of cartilage‐on‐chip for RNA extraction