bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Simple and Effective Method for Observing Starch in Whole Plant Cells and in Raw and Processed Food Ingredients
Authors: Parker Mary L. , Ryden Peter , Wilde Peter J. , Edwards Cathrina H. 
Published: October 2020

AbstractA method is described which uses cyclohexanediaminetetraacetic acid (CDTA) to produce numerous separated whole cells from plant tissue. CDTA chelates divalent cations that cross‐link the pectic polysaccharides of the middle lamella, allowing gentle separation of the cells without harsh physical treatments. These individual cells are ideal for observing starch granules in situ by microscopy without the requirement for fixation, embedding, sectioning, or prior starch extraction. Starch can easily be observed either unstained, or by polarizing optics, or after staining with iodide (I2/KI). Staining with I2/KI in combination with polarizing optics gives information on polarizing colors that indicate compositional differences within granules. Examples of the starch complement in developing, mature, and cooked rr wrinkled pea cells, and in banana and potato tissue are shown. The CDTA‐separation method is ideal for the survey of starch mutants and other cell components as it preserves cytoplasmic organization and prevents microbial degradation during storage.
Radiolabeling strategies and pharmacokinetic studies for metal based nanotheranostics
Authors: Ranjbar Bahadori Shahab , Mulgaonkar Aditi , Hart Ryan , Wu Cheng‐Yang , Zhang Dianbo , Pillai Anil , Hao Yaowu , Sun Xiankai 
Published: October 2020

AbstractRadiolabeled metal‐based nanoparticles (MNPs) have drawn considerable attention in the fields of nuclear medicine and molecular imaging, drug delivery, and radiation therapy, given the fact that they can be potentially used as diagnostic imaging and/or therapeutic agents, or even as theranostic combinations. Here, we present a systematic review on recent advances in the design and synthesis of MNPs with major focuses on their radiolabeling strategies and the determinants of their in vivo pharmacokinetics, and together how their intended applications would be impacted. For clarification, we categorize all reported radiolabeling strategies for MNPs into indirect and direct approaches. While indirect labeling simply refers to the use of bifunctional chelators or prosthetic groups conjugated to MNPs for post‐synthesis labeling with radionuclides, we found that many practical direct labeling methodologies have been developed to incorporate radionuclides into the MNP core without using extra reagents, including chemisorption, radiochemical doping, hadronic bombardment, encapsulation, and isotope or cation exchange. From the perspective of practical use, a few relevant examples are presented and discussed in terms of their pros and cons. We further reviewed the determinants of in vivo pharmacokinetic parameters of MNPs, including factors influencing their in vivo absorption, distribution, metabolism, and elimination, and discussed the challenges and opportunities in the development of radiolabeled MNPs for in vivo biomedical applications. Taken together, we believe the cumulative advancement summarized in this review would provide a general guidance in the field for design and synthesis of radiolabeled MNPs towards practical realization of their much desired theranostic capabilities.This article is categorized under:
Nanotechnology Approaches to Biology > Nanoscale Systems in Biology
Diagnostic Tools > Diagnostic Nanodevices
Therapeutic Approaches and Drug Discovery > Nanomedicine for Oncologic Disease
On three-point functions in ABJM and the latitude Wilson loop
Authors: Bianchi Marco S. 
Published: October 2020

Abstract
I consider three-point functions of twist-one operators in ABJM at weak coupling. I compute the structure constant of correlators involving one twist-one un-protected operator and two protected ones for a few finite values of the spin, up to two-loop order. As an application I enforce a limit on the gauge group ranks, in which I relate the structure constant for three chiral primary operators to the expectation value of a supersymmetric Wilson loop. Such a relation is then used to perform a successful five-loop test on the matrix model conjectured to describe the supersymmetric Wilson loop.
Microscopic Derivation of the Fröhlich Hamiltonian for the Bose Polaron in the Mean-Field Limit
Authors: Myśliwy Krzysztof , Seiringer Robert 
Published: October 2020

AbstractWe consider the quantum mechanical many-body problem of a single impurity particle immersed in a weakly interacting Bose gas. The impurity interacts with the bosons via a two-body potential. We study the Hamiltonian of this system in the mean-field limit and rigorously show that, at low energies, the problem is well described by the Fröhlich polaron model.
Differential regulation of osteopontin and CD44 correlates with infertility status in PCOS patients
Authors: Paravati R. , De Mello N. , Onyido E. K. , Francis L. W. , Brüsehafer K. , Younas K. , Spencer-Harty S. , Conlan R. S. , Gonzalez D. , Margarit Lavinia 
Published: October 2020

Abstract
Endometrial receptivity is mediated by adhesion molecules at the endometrium-trophoblast interface where osteopontin (OPN) and CD44 form a protein complex that plays an important role in embryo recognition. Here, we undertook a prospective study investigating the expression and regulation of OPN and CD44 in 50 fertile and 31 infertile ovulatory polycystic ovarian syndrome (PCOS) patients in the proliferative and secretory phases of the natural menstrual cycle and in 12 infertile anovulatory PCOS patients. Endometrial biopsies and blood samples were evaluated for expression of OPN and CD44 using RT-PCR, immunohistochemistry and ELISA analysis to determine circulating levels of OPN, CD44, TNF-α, IFN-γ and OPN and CD44 levels in biopsy media. Our findings highlighted an increased level of circulating OPN and CD44 in serum from infertile patients that inversely correlated with expression levels in endometrial tissue and positively correlated with levels secreted into biopsy media. OPN and CD44 levels positively correlated to each other in serum and media from fertile and PCOS patients, as well as to circulating TNF-α and IFN-γ. In vitro analysis revealed that hormone treatment induced recruitment of ERα to the OPN and CD44 promoters with a concomitant increase in the expression of these genes. In infertile patients, inflammatory cytokines led to recruitment of NF-κB and STAT1 proteins to the OPN and CD44 promoters, resulting in their overexpression. These observations suggest that the endometrial epithelial OPN-CD44 adhesion complex is deficient in ovulatory PCOS patients and displays an altered stoichiometry in anovulatory patients, which in both cases may perturb apposition. This, together with elevated circulating and local secreted levels of these proteins, may hinder endometrium-trophoblast interactions by saturating OPN and CD44 receptors on the surface of the blastocyst, thereby contributing to the infertility associated with ovulating PCOS patients.

Key messages
• Endometrial epithelial OPN-CD44 adhesion complex levels are deficient in ovulatory PCOS patients contributing to the endometrial infertility associated with ovulating PCOS patients.
• Circulating levels of OPN, CD44 and inflammatory cytokines TNF-α and IFN-γ are altered in infertile PCOS patients.
• Increased levels of both OPN and CD44 in biopsy media and serum inversely correlate with endometrial expression of these markers in endometrial tissue.
• In infertile PCOS patients, high levels of oestrogens and inflammatory cytokines stimulate the recruitment of transcription factors to the OPN and CD44 promoters to enhance gene transcription.
• Our study identifies a novel crosstalk between the CD44-OPN adhesion complex, ERα, STAT1 and NF-κB pathways modulating endometrial receptivity.
No detrimental effect of a positive family history on postoperative upgrading and upstaging in men with low risk and favourable intermediate-risk prostate cancer: implications for active surveillance
Authors: Herkommer Kathleen , Maier Nikola , Ankerst Donna P. , Schiele Stefan , Gschwend Jürgen E. , Meissner Valentin H. 
Published: October 2020

Abstract
Purpose
To assess whether a first-degree family history or a fatal family history of prostate cancer (PCa) are associated with postoperative upgrading and upstaging among men with low risk and favourable intermediate-risk (FIR) PCa and to provide guidance on clinical decision making for active surveillance (AS) in this patient population.

Methods
Participants in the German Familial Prostate Cancer database diagnosed from 1994 to 2019 with (1) low risk (clinical T1c–T2a, biopsy Gleason Grade Group (GGG) 1, PSA < 10 ng/ml), (2) Gleason 6 FIR (clinical T1c–T2a, GGG 1, PSA 10–20 ng/ml), and (3) Gleason 3 + 4 FIR (clinical T1c–T2a, GGG 2, PSA < 10 ng/ml) PCa who were subsequently treated with radical prostatectomy (RP) were analysed for upgrading, defined as postoperative GGG 3 tumour or upstaging, defined as pT3–pT4 or pN1 disease at RP. Logistic regression analysis was used to assess whether PCa family history was associated with postoperative upgrading or upstaging.

Results
Among 4091 men who underwent RP, mean age at surgery was 64.4 (SD 6.7) years, 24.7% reported a family history, and 3.4% a fatal family history. Neither family history nor fatal family history were associated with upgrading or upstaging at low risk, Gleason 6 FIR, and Gleason 3 + 4 FIR PCa patients.

Conclusion
Results from the current study indicated no detrimental effect of family history on postoperative upgrading or upstaging. Therefore, a positive family history or fatal family history of PCa in FIR PCa patients should not be a reason to refrain from AS in men otherwise suitable.
Darcy, Forchheimer, Brinkman and Richards: classical hydromechanical equations and their significance in the light of the TPM
Authors: Ehlers Wolfgang 
Published: October 2020

AbstractIn hydromechanical applications, Darcy, Brinkman, Forchheimer and Richards equations play a central role when porous media flow under saturated and unsaturated conditions has to be investigated. While Darcy, Brinkman, Forchheimer and Richards found their equations mainly on the basis of flow observations in field and laboratory experiments, the modern Theory of Porous Media allows for a scientific view at these equations on the basis of precise continuum mechanical and thermodynamical investigations. The present article aims at commenting the classical equations and at deriving their counterparts by the use of the thermodynamical consistent Theory of Porous Media. This procedure will prove that the classical equations are valid under certain restrictions and that extended equations exist valid for arbitrary cases in their field.
How I do it: minimally invasive resection of a sub-ependymoma of the fourth ventricle
Authors: Corniola Marco V. , Meling Torstein R. 
Published: October 2020

Abstract
Background
A 54-year-old female was referred to our clinic with a lesion of the lower fourth ventricle extending to the median aperture. Here, we report the use a minimally invasive sub-occipital approach (MISA) as a safe and effective surgical management.

Method
We performed a MISA using a short midline incision and a 1-cm sub-occipital craniectomy. Dissection of the lesion was performed, and “en bloc” resection could be achieved. The lesion was confirmed to be a grade I sub-ependymoma.

Conclusion
MISA can be safely used when confronted to a lesion of the lower fourth ventricle.
Identification of possible dynamical drivers for long-term changes in temperature and rainfall patterns over Europe
Authors: Hoffmann Peter , Spekat Arne 
Published: October 2020

AbstractThis study looks into the question to what extent long-term change patterns of observed temperature and rainfall over Europe can be attributed to dynamical causes, in other words: Are the observed changes due to a change in frequency of the patterns or have the patterns’ dynamical properties changed? By using a combination of daily meteorological data and a European weather-type classification, the long-term monthly mean temperature and precipitation were calculated for each weather-type. Subsequently, the observed weather-type sequences were used to construct analogue time series for temperature and precipitation which only include the dynamical component of the long-term variability since 1961. The results show that only a fraction of about 20% of the past temperature rise since 1990, which for example amounted to 1 °C at the Potsdam Climate Station can be explained by dynamical changes, i.e. most of the weather-types have become warmer. Concerning long-term changes of seasonal rainfall patterns, a fraction of more than 60% is considerably higher. Moreover, the results indicate that for rainfall compared with temperature, the decadal variability and trends of the dynamical component follow the observed ones much stronger. Consequently, most of the explained seasonal rainfall variances can be linked to changes in weather-type sequences in Potsdam and over Europe. The dynamical contribution to long-term changes in annual and seasonal rainfall patterns dominates due to the fact that the alternation of wet and dry weather-types (e.g. the types Trough or High pressure over Central Europe), their frequencies and duration has significantly changed in the last decades.
Association of lipid profiles with severity and outcome of acute ischemic stroke in patients with and without chronic kidney disease
Authors: Zhang Ailing , Deng Wenjing , Zhang Bin , Ren Mengyang , Tian Long , Ge Jihui , Bai Jinjuan , Hu Hao , Cui Ling 
Published: October 2020

Abstract
Background
Contribution of lipid profiles to stroke severity and outcome was inconclusive, whether chronic kidney disease (CKD) (estimated glomerular filtration rate < 60 mL/min/1.73 m2) affects the association has not been investigated. We aim to evaluate this relationship.

Methods
A retrospective study of consecutive acute ischemic stroke patients was performed. We assessed the risk of severe stroke with the National Institutes of Health Stroke Scale (NIHSS) ≥ 5 at admission and poor outcome with the modified Rankin Scale (mRS) ≥ 3 at discharge. Multivariate stepwise logistic regression models were adopted to study interaction and independent association of lipid components with stroke severity and outcome according to lipid level quartiles by CKD stratification.

Results
Among the 875 included patients (mean age 64.9 years, 67.8% males), 213 (24.3%) presented with CKD. Elevated low-density lipoprotein cholesterol (LDL-C) was independently associated with severe stroke in patients with CKD (P for trend = 0.033) than in those without CKD (P for trend = 0.121). The association between the level of LDL-C and stroke severity was appreciably modified by CKD (Pinteraction = 0.013). Compared with without CKD patients in the lowest LDL-C quartile, the multivariable-adjusted risk of severe stroke increased significantly by 2.9-fold (95% CI 1.48–5.74) in patients with CKD in the highest LDL-C quartile. No significant association was observed between lipid components and early outcome in patients with and without CKD.

Conclusion
LDL-C levels are positively associated with stroke severity in only patients with CKD, with an interactive impact of LDL-C and CKD on ischemic stroke in the acute phase.
Physical and chemical characteristics of slag produced during Pb refining and the environmental risk associated with the storage of slag
Authors: Kicińska Alicja 
Published: October 2020

AbstractMetallurgical waste originating from the Zn and Pb refining process presents varying chemical composition and physical properties and contains varying quantities of pollutants. In the study, both fresh and weathered samples of production slag from the final Pb refining process were used to determine its physical parameters and chemical composition as well as to perform an environmental risk assessment (RAC, mRAC) related to its storage. This paper focuses on determining parameters such as natural humidity (1.8%) and bulk density (1267 kg/m3) of the slag. Also, its toxicity was analysed using bio-tests, its pH was measured (11.92) and the content of sulphates (3.5 wt%), chlorides (0.3 wt%) and selected heavy metals (Cd, Cu, Fe, Mn, Zn, Pb) was determined. The Individual Contamination Factor was determined, yielding the following order of the hazard level: Pb > Cu > Zn > Cd > Fe > Mn. Based on the mobility of metals determined using the Mobility Factor, it was concluded that the hazard level followed the sequence Cd > Pb > Zn > Mn > Fe > Cu. The obtained water leaching results were compared to the values found in the legal regulations in force. Based on this comparison, it was established that the slag studied constitutes toxic waste containing considerable quantities of sulphides and chlorides. The material is also a considerable source of readily leaching elements (Cd, Zn and Pb), and thus any product created using the slag may become hazardous to the environment. Also, the waste studied must not be used in the form in which it is currently stored due to the leaching of particularly toxic elements (Cd and Pb) in water solutions with increasing acidity.
Hybrid evolutionary oligopolies and the dynamics of corporate social responsibility
Authors: Tichý Tomáš , Radi Davide , Lamantia Fabio 
Published: October 2020

AbstractThe diffusion ofcorporate social responsibilityis investigated by employing a hybrid evolutionary game where a firm chooses between being either socially responsible, which implies devoting a fraction of its profit to social projects, or non-socially responsible. Consumers prize socially responsible companies by paying a higher reservation price for their products. The hybrid evolutionary framework is characterized by a quantity dynamics that describes the oligopolistic competition given firms’ belief about the composition of the industry. At regular intervals of time, this belief is endogenously updated by a retrospective comparison on the profits obtained and on the basis of an evolutionary mechanism. Assuming that firms are Nash players, that is at each instant of time they produce the Nash equilibrium-in-belief quantity, the investigation of the model reveals that an industry homogeneously populated by socially responsible firms is a stable equilibrium when the fraction of profits earmarked for socially responsible activities is sufficiently limited. However, the extra marginal profits of a socially responsible firm are reduced when the number of competitors increases, impeding the diffusion of socially responsible companies. In particular, the trade-off between a higher net margin on sales obtained by socially responsible firms and a lower level of production that reduces the profit gap between a socially responsible firm and the rest of the market shows that an increased size of the industry favors mixed oligopolies. Moreover, imposing the hypothesis of neutrality of CSR activities, the model reveals that being socially responsible is an evolutionarily stable strategy for firms and is convenient for customers. Relaxing the hypothesis of Nash players by introducing boundedly rational firms that decide their level of production according to a partial adjustment toward the best reply, the robustness of these results is confirmed.
Gibbs States, Algebraic Dynamics and Generalized Riesz Systems
Authors: Bagarello F. , Inoue H. , Trapani C. 
Published: October 2020

AbstractIn PT-quantum mechanics the generator of the dynamics of a physical system is not necessarily a self-adjoint Hamiltonian. It is now clear that this choice does not prevent to get a unitary time evolution and a real spectrum of the Hamiltonian, even if, most of the times, one is forced to deal with biorthogonal sets rather than with on orthonormal basis of eigenvectors. In this paper we consider some extended versions of the Heisenberg algebraic dynamics and we relate this analysis to some generalized version of Gibbs states and to their related KMS-like conditions. We also discuss some preliminary aspects of the Tomita–Takesaki theory in our context.
Landfall After the Perfect Storm: Cohort Differences in the Relationship Between Debt and Risk of Heart Attack
Authors: O’Rand Angela M. , Hamil-Luker Jenifer 
Published: October 2020

Abstract
Analyses of the Health and Retirement Study (HRS) between 1992 and 2014 compare the relationship between different levels and forms of debt and heart attack risk trajectories across four cohorts. Although all cohorts experienced growing household debt, including the increase of both secured and unsecured debt, they nevertheless encountered different economic opportunity structures and crises at sensitive times in their life courses, with implications for heart attack risk trajectories. Results from frailty hazards models reveal that unsecured debt is associated with increased risk of heart attack across all cohorts. Higher levels of housing debt, however, predict higher rates of heart attack among only the earlier cohorts. Heart attack risk trajectories for Baby Boomers with high levels of housing debt are lower than those of same-aged peers with no housing debt. Thus, the relationship between debt and heart attack varies by level and form of debt across cohorts but distinguishes Baby Boomer cohorts based on their diverse exposures to volatile housing market conditions over the sensitive household formation period of the life course.
Absorption Pigment Cores for Pearlescent Pigments
Authors: Matejdes Marián , Hausner Josef , Grüner Michael , Kaupp Günter , Breu Josef 
Published: October 2020

AbstractA lustrous appearance and interference-based colors make pearlescent pigments attractive for use in applications such as automotive paints, plastics, consumer electronics, and cosmetics. A combination of interference and absorption in the visible light spectrum improves significantly the hiding power as well as the color strength of pearlescent pigments while potentially extending their color range. The aim of the present study was to introduce synthetic fluorohectorites, having an appreciable diameter (~20 μm) and aspect ratio (~1000), as promising colored cores for pearlescent pigments. Fluorohectorites can adopt a variety of colors by ion-exchange reaction with cationic organic dyes of high absorption coefficient. Unlike related dye-exchanged natural montmorillonite clays, which undergo acid activation accompanied by release of dye at low pH, as is required for subsequent coating with TiO2 in an environment with low pH and elevated temperature, no leaching was observed with dye-exchanged synthetic fluorohectorites ([Na0.5]int.[Mg2.5Li0.5]oct.[Si4]tet.O10F2). Due to its significantly greater layer charge, more organic dye molecules were adsorbed per volume of the fluorohectorite than for montmorillonite. Consequently, the free volume available in the interlayer space for H3O+ diffusion was less for synthetic fluorohectorite than for montmorillonite. Acid attack via interlayer space was, therefore, retarded significantly for fluorohectorite. Acid attack from the external edges of synthetic fluorohectorites was in the range of conventionally applied mica pigment core (fluorophlogopite, ([K]int.[Mg3]oct.[AlSi3]tet.O10(F,OH)2) because of the comparable large diameter of the platelets. Montmorillonite, however, occurs with particle diameters typically <200 nm and the much increased relative contribution of edges to the total surface area also makes them more prone to acid attack and concomitant leaching. Aside from leaching stability, the confinement of organic dyes in the interlayer space restricts rotational and vibrational motions, which in turn stabilizes the dyes typically by ~100°C against thermal decomposition as compared to chloride salts of the dyes.