bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparing the EQ‐5D‐5L crosswalks and value sets for England, the Netherlands and Spain: Exploring their impact on cost‐utility results
Authors: Ben Ângela , Finch Aureliano Paolo , van Dongen Johanna M. , de Wit Maartje , van Dijk Susan E.M. , Snoek Frank J. , Adriaanse Marcel C. , van Tulder Maurits W. , Bosmans Judith E. 
Published: February 2020

AbstractThis study compares the five‐level EuroQol five‐dimension questionnaire (EQ‐5D‐5L) crosswalks and the 5L value sets for England, the Netherlands, and Spain and explores the implication of using one or the other for the results of cost–utility analyses. Data from two randomized controlled trials in depression and diabetes were used. Utility value distributions were compared, and mean differences in utility values between the EQ‐5D‐5L crosswalk and the 5L value set were described by country. Quality‐adjusted life years (QALYs) were calculated using the area‐under‐the‐curve method. Incremental cost‐effectiveness ratios (ICERs) were calculated, and uncertainty around ICERs was estimated using bootstrapping and graphically shown in cost‐effectiveness acceptability curves. For all countries investigated, utility value distributions differed between the EQ‐5D‐5L crosswalk and 5L value set. In both case studies, mean utility values were lower for the EQ‐5D‐5L crosswalk compared with the 5L value set in England and Spain, but higher in the Netherlands. However, these differences in utility values did not translate into relevant differences across utility estimation methods in incremental QALYs and the interventions' probability of cost‐effectiveness. Thus, our results suggest that EQ‐5D‐5L crosswalks and 5L value sets can be used interchangeably in patients affected by mild or moderate conditions. Further research is needed to establish whether these findings are generalizable to economic evaluations among severely ill patients.
Risk of second primary cancer in oral squamous cell carcinoma
Authors: Mroueh Rayan , Nevala Aapeli , Haapaniemi Aaro , Pitkäniemi Janne , Salo Tuula , Mäkitie Antti A. 
Published: February 2020

AbstractBackgroundThe incidence and survival of oral squamous cell carcinoma (OSCC) patients have increased in recent years. Understanding their long‐term survival aspects is essential for optimal treatment and follow‐up planning. Almost one in five cancers diagnosed occurs nowadays in individuals with a previous diagnosis of cancer.MethodsPatients diagnosed with primary OSCC during 1953‐2015 were retrieved from the Finnish Cancer Registry. Both standardized incidence ratios (SIR) and excess absolute risk (EAR) per 1000 person‐years at risk (PYR) of second primary cancer (SPC) were calculated relative to the general population.ResultsAmong 6602 first primary OSCC patients there were 640 (10%) SPCs. The SIR for SPCs was 1.85 (95% CI: 1.71‐1.99, P < .001) corresponding to an EAR of 8.78 (95% CI: 7.29‐10.26).ConclusionsHealth care professionals should be aware of the second primary cancer risk after management of primary OSCC and patients need to be counseled about this phenomenon.
First Principles Investigation of Cold Curves of Metals
Authors: Eidelstein Eitan , Barzilai Shmuel , Curtarolo Stefano , Levy Ohad 
Published: February 2020

AbstractThe rapid development of better high pressure experimental techniques combined with efficient and accurate density functional calculations of the structural properties of materials provide a new avenue to promote the study of materials at high pressures, which is currently based mostly on simple phenomenological modelling. The progress of experimental results into higher‐pressure regimes represents a challenge to the phenomenological approaches, which can be addressed by carefully considered ab initio calculations. We present cold curves of several elements, calculated using different approximations of DFT and compare them with available experimental data. The comparison shows good agreement both in simple single phase and complex multi‐phase cases. It suggests that DFT may be used to extrapolate high pressure behaviour of materials beyond the currently possible pressure range, with a robust estimate of the accuracy of the extrapolation based on various DFT implementations.
Substance use and mothers’ neural responses to infant cues
Authors: Lowell Amanda F. , Maupin Angela N. , Landi Nicole , Potenza Marc N. , Mayes Linda C. , Rutherford Helena J. V. 
Published: February 2020

ABSTRACTSubstance use may influence mothers’ responsiveness to their infants and negatively impact the parent–infant relationship. Maternal substance use may co‐opt neural circuitry involved in caregiving, thus reducing the salience of infant cues and diminishing the sense of reward experienced by caring for infants. Gaps in understanding exist with regard to the mechanisms by which substance use operates to influence mothers’ processing of infant cues and how this translates to caregiving. Therefore, we examined how substance use might relate to maternal neural responses to infant cues using event‐related potentials (ERPs). Substance‐using (n = 29) and nonsubstance‐using (n = 29) mothers viewed photographs of infant faces and heard recordings of infant vocalizations while electroencephalography was recorded simultaneously. Three specific ERP components were used to examine initial processing of infant faces (N170) and cries (N100), and attentional allocation to infant faces and cries (P300). Substance‐using mothers did not discriminate facial affect at early encoding stages (N170), were generally slower to orient to infant cries (N100), showed heightened responses to neutral faces (P300), and failed to adaptively differentiate between high‐distress versus low‐distress cries (P300). These differences may be important to caregiving behaviors associated with the formation of mother–child attachment. Implications are discussed, as are limitations and future directions.
MicroRNA‐34a causes ceramide accumulation and effects insulin signaling pathway by targeting ceramide kinase (CERK) in aging skeletal muscle
Authors: Kukreti Himani , Amuthavalli Kottaiswamy 
Published: February 2020

AbstractAging skeletal muscle shows perturbations in metabolic functions. MicroRNAs have been shown to play a critical role in aging and metabolic functions of skeletal muscle. MicroRNA‐34a (miR‐34a) is implicated in the brain and cardiac aging, however, its role in aging muscle is unclear. We analyzed levels of miR‐34a, ceramide kinase (CERK) and other insulin signaling molecules in skeletal muscle from old mice. In addition to in vivo model, levels of these molecules were also analyzed in myoblast derived from insulin resistant (IR) humans and C2C12 myoblasts overexpressing mir‐34a. Our results show that miR‐34a is elevated in the muscles of 2‐year‐old mice and in the myoblasts of IR humans. Overexpression of miR‐34a in C2C12 myoblasts leads to alterations in the insulin signaling pathway, which were rescued by its antagonism. Our analyses revealed that miR‐34a targets CERK resulting in ceramide accumulation, activation of PP2A and the pJNK pathway in muscle and C2C12 myoblasts. Also, myostatin (Mstn) levels were increased in 2‐year‐old mouse muscle and Mstn treatment upregulated miR‐34a in C2C12 myoblasts. In addition, miR‐34a expression and ceramide levels did not increase during aging in Mstn−/− mice muscle. In summary, we, therefore, propose that Mstn levels increase in aging muscle and upregulate miR‐34a, which inhibits CERK resulting in increased ceramide levels. This ceramide accumulation activates PP2A and pJNK causing hypophosphorylation of AKT and hyperphosphorylation of IRS1 (Ser307), respectively, impairing insulin signaling pathway and eventually inhibiting the sarcolemma localization of GLUT4. These changes would result in reduced glucose uptake and insulin resistance. This study is the first to explain the phenomenon of ceramide accrual and impairment of insulin signaling pathway in aging muscle through a miR‐34a based mechanism. In conclusion, our results suggest that Mstn and miR‐34a antagonism can help ameliorate ceramide accumulation and loss of insulin sensitivity in aging skeletal muscle.
Retracted: Kinesin family member 2C aggravates the progression of hepatocellular carcinoma and interacts with competing endogenous RNA
Authors: Zhang Guo‐Pei , Shen Shun‐Li , Yu Yang , Yue Xiao , Hu Wen‐Jie , Li Shao‐Qiang 
Published: February 2020

AbstractKinesin family member 2C (KIF2C), a substantial mitotic regulator, has been verified to exert a malignant function in several cancers. However, its function in hepatocellular carcinoma (HCC) remains unclear. In this study, the expression profile of KIF2C in HCC was characterized through the dataset from the TCGA and clinical tissue microarrays containing 220 pairs of resected HCC tissues and adjacent nontumor tissues in our hospital. The results indicated that KIF2C was substantially higher expression in tumor tissues than adjacent nontumor tissues. High expression of KIF2C significantly correlated with large tumor (>5.0 cm) (P = .001) and implied a dismal postoperative overall survival (OS) (hazard ratio [HR] = 1.729; P = .002) in our cohort of patients. Gain and loss of function assays displayed that KIF2C promoted HCC cell proliferation, accelerated cell cycle progression, and impeded apoptosis. By bioinformatic tools and mechanistic investigation, we found that KIF2C interacted with various cell‐cycle‐related proteins and was significantly involved in growth‐promoting pathways. KIF2C upregulated PCNA and CDC20 expression. Subsequently, we investigated the regulation of KIF2C by competing endogenous RNA and elucidated that has‐miR‐6715a‐3p was directly bond to the 3′‐untranslated region of KIF2C through dual luciferase assays, thereby inhibiting KIF2C expression. Furthermore, the long noncoding RNA GS1‐358P8.4 was found to be a candidate of KIF2C for has‐miR‐6715a‐3p binding. HCC patients with high lncRNA‐GS1‐358P8.4 expression had shorter OS and relapse‐free survival compared to those with low expression, which was accordance with the KIF2C. Taken together, KIC2C aggravated HCC progression, it could serve as a prognostic indicator and confer a novel target for clinical treatment.
Efficient implementation of the interacting quantum atoms energy partition of the second‐order Møller–Plesset energy
Authors: Casals‐Sainz José Luis , Guevara‐Vela José Manuel , Francisco Evelio , Rocha‐Rinza Tomás , Martín Pendás Ángel 
Published: February 2020

AbstractWe describe an efficient implementation of the partition of the second‐order Møller–Plesset (MP2) correlation energy within the interacting quantum atoms (IQA) energy decomposition. We simplify the IQA integration bottleneck by considering only the occupied to virtual elements of the second order reduced density matrix, a procedure that reduces substantially the size of the two‐electron matrix, which has to be addressed. The algorithmic improvements described herein allow to perform the decomposition of the MP2 correlation energy for medium size molecular systems using moderate computational resources. We expect that the methods developed in this investigation will prove useful to understand electron correlation effects through a real space perspective.
miR‐22 represses osteoblast viability with ESR1 presenting a direct target and indirectly inactivating p38 MAPK/JNK signaling
Authors: Chen Guang‐Hua , Zhang Xin‐Le , Chen Hang , Lin Hao , Wu Hao‐Jun , Lin Han , Huang Gui‐Zhi 
Published: February 2020

AbstractBackgroundMicroRNAs (miRs) hold critical implications in the modulation of osteogenesis. This work was designed to unravel the underlying regulatory mechanism of miR‐22 during osteoblast differentiation.MethodsSynthetic miR‐22 mimics or inhibitors were transfected into preosteoblast MC3T3‐E1 cells to regulate miR‐22 expression. MTT [3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide] and flow cytometry analyses were employed to assess cell proliferation and apoptosis. A quantitative real‐time polymerase chain reaction and western blot assays were applied to measure mRNA and protein expression. Alkaline phosphatase activity and alizarin red staining were tested to further analyze cell differentiation. In silico analysis and luciferase reporter assays were utilized to identify the direct binding between miR‐22 and its potential target.ResultsMTT and flow cytometry analyses showed that miR‐22 repressed MC3T3‐E1 cell viability and promoted cell apoptosis. By detecting osteogenic‐specific molecule expression, alkaline phosphatase activity and alizarin red staining, miR‐22 was observed to suppress osteogenic differentiation of MC3T3‐E1 cells. In silico analysis and luciferase reporter assays confirmed that ESR1 is a direct target gene of miR‐22. In addition, miR‐22 expression affected the phosphorylation of p38 mitogen‐activated protein kinase and Jun N‐terminal kinase expression in MC3T3‐E1 cells.ConclusionsThe findings of the present study highlight the functional significance of miR‐22 in osteoblast differentiation and suggest its role as a possible therapeutic target in metabolic bone disorders.
Partial replacement of rock phosphate by sewage sludge ash for the production of superphosphate fertilizers
Authors: Hartmann Tobias Edward , Möller Kurt , Meyer Carsten , Müller Torsten 
Published: February 2020

AbstractThermal utilization of sewage sludge through mono‐incineration or gasification results in phosphorus (P) rich sewage sludge ash (SSA) that must be returned to agricultural production systems to fulfill the need for recycling of P resources contained in wastewater streams. As the plant‐availability of P contained in SSA is low, we propose feeding SSA directly into the production of superphosphate fertilizers, thereby opening a further pathway for the recycling of phosphorus (P) from wastewater streams to agricultural production systems by using available technologies. We carried out laboratory‐scale production of superphosphate test‐products, in which rock phosphate (RP) was partially replaced with SSA (gasification) before digestion with concentrated sulfuric acid, and evaluated these products with regard to the solubility of P in H2O and neutral ammoniumcitrate solution. We further carried out a growth‐chamber experiment (28 d) using maize (Zea mays L. cv. Sulano) as a model plant on a low P (0.4 mg PCAL 100g−1), high pH (7.6) substrate to evaluate plant P availability of the test products. Our laboratory‐scale results show that at least 8% of P from RP can be replaced by P from SSA while maintaining both the high solubility of P in the fertilizer product and the growth of maize compared to pure RP digested with concentrated sulfuric acid. Further substitution of RP through SSA decreased the total P concentration of the test products, as well as the relative amounts of P soluble in H2O and neutral ammoniumcitrate solution, which affected early plant development of maize.
Adsorption of toluene‐3,4‐dithiol on silver islands investigated by surface‐enhanced Raman spectroscopy
Authors: Plaickner Julian , Speiser Eugen , Chandola Sandhya , Esser Norbert , Singh Dheeraj K. 
Published: February 2020

AbstractThe adsorbtion of toluene‐3,4‐dithiol (TDT) molecules on Ag islands was investigated with surface‐enhanced Raman spectroscopy (SERS). The SERS spectrum was compared with the ordinary Raman spectrum of TDT, and differences in spectral features were discussed with the support of density functional theory calculations. The disappearance of all the S–H‐related bands in the SERS spectrum indicates that TDT adsorbs on Ag via both thiol groups, as supported by the emergence of a weak band at 223 cm−1 attributed to Ag–S stretching. Signatures of Ag–S–C complexes suggest that the adsorption is partially disordered. The observation of all the vibrational modes involving the methyl group indicates that the methyl group is exposed, offering new functionalization possibilities of the surface.
Enhancement of Fat Oxidation during Submaximal Exercise in Sedentary Persons: Variations by Medium‐Chain Fatty Acid Composition and Age Group
Authors: Nosaka Naohisa , Tsujino Shougo , Honda Kazumitsu , Suemitsu Hiromi , Kato Kazuhiko 
Published: February 2020

AbstractPrevious studies in recreational and trained athletes aged mostly in their 20s have reported that short‐term ingestion of medium‐chain triacylglycerols (MCT) enhances fat oxidation (FAO) during submaximal exercise. However, whether the FAO‐enhancing effect of MCT with a different composition of medium‐chain fatty acids (MCFA) occurs in older sedentary persons is unclear. The present study investigated the effect of MCT ingestion with different proportions of MCFA in sedentary participants in their 40's and 50's. Participants ingested 0 g of MCT (control), 6 g of octanoic acid‐rich MCT (OAR), or 6 g of decanoic acid‐rich MCT (DAR) for 14 days separated by a 14‐day washout period in random order. Cumulative FAO (Fcv) during submaximal, fixed, and incremental exercise was evaluated at workload from 20 W to the appearance of a ventilation threshold (VT). During the 20 W fixed‐load exercise, Fcv was significantly (p < 0.05) higher in the OAR than in the control. At appearance of VT, intervention effect of power output was significantly higher in the OAR and DAR than in the control. In a subgroup analysis by age, intervention effects of maximal FAO rate and oxygen uptake in the upper age subgroup were higher in the OAR and DAR than in the control. In a pooled analysis with age subgroup and diet, the integrated pooled estimate of Fcv during submaximal exercise was significantly higher in 6 g of MCT ingestion than 0 g ingestion. Our data show that the effect of MCT might differ depending on the age group and the proportion of MCFA, while MCT could enhance FAO during submaximal exercise.
Projected increase of the East Asian summer monsoon (Meiyu) in Taiwan by climate models with variable performance
Authors: Tung Yu‐Shiang , Wang S.‐Y. Simon , Chu Jung‐Lien , Wu Chi‐Hua , Chen Yung‐Ming , Cheng Chao‐Tzuen , Lin Lee‐Yaw 
Published: February 2020

AbstractThe active phase of the East Asian summer monsoon (EASM) in Taiwan during May and June, known as Meiyu, produces substantial precipitation for water uses in all sectors of society. Following a companion study that analysed the historical increase in the Meiyu precipitation, the present study conducted model evaluation and diagnosis based on the EASM lifecycle over Taiwan. Higher and lower skill groups were identified from 17 Couple Model Intercomparison Project Phase 5 (CMIP5) models, with five models in each group. Despite the difference in model performance, both groups projected a substantial increase in the Meiyu precipitation over Taiwan. In the higher skill group, weak circulation changes and reduced low‐level convergence point to a synoptically unfavourable condition for precipitation. In the lower skill group, intensified low‐level southwesterly winds associated with a deepened upper level trough enhance moisture pooling. Thus, the projected increase in Meiyu precipitation will likely occur through the combined effects of (1) the extension of a strengthened North Pacific anticyclone enhancing southwesterlies; and (2) more systematically, the Clausius–Clapeyron relationship that increases precipitation intensity in a warmer climate. The overall increase in the Meiyu precipitation projected by climate models of variable performance supports the observed tendency toward more intense rainfall in Taiwan and puts its early June 2017 extreme precipitation events into perspective.
Identification of two compound heterozygous VPS13A large deletions in chorea‐acanthocytosis only by protein and quantitative DNA analysis
Authors: Spieler Derek , Velayos‐Baeza Antonio , Mühlbäck Alžbeta , Castrop Florian , Maegerlein Christian , Slotta‐Huspenina Julia , Bader Benedikt , Haslinger Bernhard , Danek Adrian 
Published: February 2020

AbstractBackgroundChorea‐acanthocytosis (ChAc; OMIM #200150) is a rare autosomal recessive condition with onset in early adulthood that is caused by mutations in the vacuolar protein sorting 13A (VPS13A) gene encoding chorein. Several diagnostic genomic DNA (gDNA) sequencing approaches are widely used. However, their limitations appear not to be acknowledged thoroughly enough.MethodsClinically, we deployed magnetic resonance imaging, blood smear analysis, and clinical chemistry for the index patient's characterization. The molecular analysis of the index patient next to his parents covered genomic DNA (gDNA) sequencing approaches, RNA/cDNA sequencing, and chorein specific Western blot.ResultsWe report a 33‐year‐old male patient without functional protein due to compound heterozygosity for two VPS13A large deletions of 1168 and 1823 base pairs (bp) affecting, respectively, exons 8 and 9, and exon 13. To our knowledge, this represents the first ChAc case with two compound heterozygous large deletions identified so far. Of note, standard genomic DNA (gDNA) Sanger sequencing approaches alone yielded false negative findings.ConclusionOur case demonstrates the need to carry out detection of chorein in patients suspected of having ChAc as a helpful and potentially decisive tool to establish diagnosis. Furthermore, the course of the molecular analysis in this case discloses diagnostic pitfalls in detecting some variations, such as deletions, using only standard genomic DNA (gDNA) Sanger sequencing approaches and exemplifies alternative methods, such as RNA/cDNA sequencing or qRT‐PCR analysis, necessary to avoid false negative results.
Methylation‐based algorithms for diagnosis: experience from neuro‐oncology
Authors: Pickles Jessica C , Stone Thomas J , Jacques Thomas S 
Published: February 2020

AbstractBrain tumours are the most common tumour‐related cause of death in young people. Survivors are at risk of significant disability, at least in part related to the effects of treatment. Therefore, there is a need for a precise diagnosis that stratifies patients for the most suitable treatment, matched to the underlying biology of their tumour. Although traditional histopathology has been accurate in predicting treatment responses in many cases, molecular profiling has revealed a remarkable, previously unappreciated, level of biological complexity in the classification of these tumours. Among different molecular technologies, DNA methylation profiling has had the most pronounced impact on brain tumour classification. Furthermore, using machine learning‐based algorithms, DNA methylation profiling is changing diagnostic practice. This can be regarded as an exemplar for how molecular pathology can influence diagnostic practice and illustrates some of the unanticipated benefits and risks. © 2020 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.