bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reliability and validity of the Spanish (Spain) version of the consultation and relational empathy measure in primary care
Authors: García del Barrio Loreto , Rodríguez-Díez Cristina , Martín-Lanas Raquel , Costa Patricio , Costa Manual J , Díez Nieves 
Published: November 2020

AbstractBackgroundEmpathy is an essential competence in the medical field. There are no validated patient-rated empathy measures in Spanish (Spain). The Consultation and Relational Empathy (CARE) measure is a widely used patient-rated measure of physician empathy.ObjectiveTo analyse the reliability and validity of the Spanish (Spain) version of the CARE measure in primary care.MethodsAfter translation, back translation and pilot testing, a convenient sample of 369 patients recruited through 21 primary care physicians in five primary care centres in Pamplona (Navarre, Spain) completed the Spanish (Spain) CARE (Sp-CARE) measure. The number of ‘does not apply’ or blank responses was calculated to assess acceptability. We analysed internal reliability by means of Cronbach’s alpha and ordinal alpha and homogeneity with corrected item-total correlations. The construct validity was examined by confirmatory factor analysis (CFA) and concurrent validity by Spearman’s correlation.ResultsWe observed high acceptability; only 37 (1%) responses were marked ‘does not apply’ and only 3 (0.08%) were left blank. Cronbach’s alpha and ordinal alpha for the Sp-CARE measure were 0.953 and 0.970, respectively, and all corrected item-total correlations exceeded the accepted cut of 0.30, demonstrating high internal reliability and homogeneity. CFA corroborated the one-factor structure proposed in the original version. The Sp-CARE measure total score was significantly correlated with overall patient satisfaction (Spearman’s rho 0.45, P &lt; 0.001).ConclusionThe results support the reliability and validity of the Sp-CARE measure as a patient-rated empathy measure in the primary care setting.
The biology of thermoacidophilic archaea from the order Sulfolobales
Authors: Lewis April M , Recalde Alejandra , Bräsen Christopher , Counts James A , Nussbaum Phillip , Bost Jan , Schocke Larissa , Shen Lu , Willard Daniel J , Quax Tessa E F , Peeters Eveline , Siebers Bettina , Albers Sonja-Verena , Kelly Robert M 
Published: November 2020

ABSTRACT
Thermoacidophilic archaea belonging to the order Sulfolobales thrive in extreme biotopes, such as sulfuric hot springs and ore deposits. These microorganisms have been model systems for understanding life in extreme environments, as well as for probing the evolution of both molecular genetic processes and central metabolic pathways. Thermoacidophiles, such as the Sulfolobales, use typical microbial responses to persist in hot acid (e.g. motility, stress response, biofilm formation), albeit with some unusual twists. They also exhibit unique physiological features, including iron and sulfur chemolithoautotrophy, that differentiate them from much of the microbial world. Although first discovered &gt;50 years ago, it was not until recently that genome sequence data and facile genetic tools have been developed for species in the Sulfolobales. These advances have not only opened up ways to further probe novel features of these microbes but also paved the way for their potential biotechnological applications. Discussed here are the nuances of the thermoacidophilic lifestyle of the Sulfolobales, including their evolutionary placement, cell biology, survival strategies, genetic tools, metabolic processes and physiological attributes together with how these characteristics make thermoacidophiles ideal platforms for specialized industrial processes.
Genomics of Recombination Rate Variation in Temperature-EvolvedDrosophila melanogasterPopulations
Authors: Winbush Ari , Singh Nadia D 
Published: November 2020

AbstractMeiotic recombination is a critical process that ensures proper segregation of chromosome homologs through DNA double-strand break repair mechanisms. Rates of recombination are highly variable among various taxa, within species, and within genomes with far-reaching evolutionary and genomic consequences. The genetic basis of recombination rate variation is therefore crucial in the study of evolutionary biology but remains poorly understood. In this study, we took advantage of a set of experimental temperature-evolved populations of Drosophila melanogaster with heritable differences in recombination rates depending on the temperature regime in which they evolved. We performed whole-genome sequencing and identified several chromosomal regions that appear to be divergent depending on temperature regime. In addition, we identify a set of single-nucleotide polymorphisms and associated genes with significant differences in allele frequency when the different temperature populations are compared. Further refinement of these gene candidates emphasizing those expressed in the ovary and associated with DNA binding reveals numerous potential candidate genes such as Hr38, EcR, and mamo responsible for observed differences in recombination rates in these experimental evolution lines thus providing insight into the genetic basis of recombination rate variation.
Majority of alpha2,6-sialylated glycans in the adult mouse brain exist inO-glycans: SALSA-MS analysis for knockout mice of alpha2,6-sialyltransferase genes
Authors: Ohmi Yuhsuke , Nishikaze Takashi , Kitaura Yoko , Ito Takako , Yamamoto Satoko , Sugiyama Fumihiro , Matsuyama Makoto , Takahashi Yoshimasa , Takeda Akira , Kawahara Toshio , Okajima Tetsuya , Furukawa Keiko , Furukawa Koichi 
Published: November 2020

AbstractSialic acids are unique sugars with negative charge and exert various biological functions such as regulation of immune systems, maintenance of nerve tissues and expression of malignant properties of cancers. Alpha 2,6 sialylated N-glycans, one of representative sialylation forms, are synthesized by St6gal1 or St6gal2 gene products in humans and mice. Previously, it has been reported that St6gal1 gene is ubiquitously expressed in almost all tissues. On the other hand, St6gal2 gene is expressed mainly in the embryonic and perinatal stages of brain tissues. However, roles of St6gal2 gene have not been clarified. Expression profiles of N-glycans with terminal α2,6 sialic acid generated by St6gal gene products in the brain have never been directly studied. Using conventional lectin blotting and novel sialic acid linkage-specific alkylamidationmass spectrometry method (SALSA-MS), we investigated the function and expression of St6gal genes and profiles of their products in the adult mouse brain by establishing KO mice lacking St6gal1 gene, St6gal2 gene, or both of them (double knockout). Consequently, α2,6-sialylated N-glycans were scarcely detected in adult mouse brain tissues, and a majority of α2,6-sialylated glycans found in the mouse brain were O-linked glycans. The majority of these α2,6-sialylated O-glycans were shown to be disialyl-T antigen and sialyl-(6)T antigen by mass spectrometry analysis. Moreover, it was revealed that a few α2,6-sialylated N-glycans were produced by the action of St6gal1 gene, despite both St6gal1 and St6gal2 genes being expressed in the adult mouse brain. In the future, where and how sialylated O-linked glycoproteins function in the brain tissue remains to be clarified.
Association Between Fatty Liver and Cirrhosis, Hepatocellular Carcinoma, and Hepatitis B Surface Antigen Seroclearance in Chronic Hepatitis B
Authors: Li Jie , Yang Hwai-I , Yeh Ming-Lun , Le Michael H , Le An K , Yeo Yee Hui , Dai Chia-Yen , Barnett Scott , Zhang Jian Q , Huang Jee-Fu , Trinh Huy N , Wong Christopher , Wong Clifford , Hoang Joseph K , Cheung Ramsey , Yu Ming-Lung , Nguyen Mindie H 
Published: November 2020

AbstractBackgroundChronic hepatitis B (CHB) and fatty liver (FL) are common, natural history data on concurrent FL and CHB (FL-CHB) are limited. This study aimed to evaluate the effect of FL on cirrhosis, hepatocellular carcinoma (HCC), and hepatitis B surface antigen (HBsAg) seroclearance incidence in CHB patients.MethodsIn a retrospective cohort study of 6786 adult CHB patients, we used propensity score matching (PSM) to balance the FL-CHB and non-FL CHB groups. Kaplan-Meier methods were used to compare cumulative cirrhosis, HCC, and HBsAg seroclearance rates between subgroups.ResultsBefore PSM, compared to non-FL CHB, FL-CHB patients had lower 10-year cumulative rates of cirrhosis, HCC, and a higher HBsAg seroclearance rate. Similar results were found in the matched FL-CHB and non-FL CHB patients, as well as in the antiviral-treated PSM cohort. Cox proportional hazards model indicated FL to remain significantly and strongly associated with lower risk of cirrhosis and HCC (hazard ratio [HR], 0.19 [95% confidence interval {CI}, .12–.33], P &lt; .001 and HR, 0.21 [95% CI, .09–.51], P = .001, respectively) in antiviral-treated patients but not in untreated patients.ConclusionsFL was significantly associated with lower cirrhosis and HCC risk and higher HBsAg seroclearance. Further studies are needed to confirm our funding and investigate the mechanisms underlying the impact of FL on CHB.
Cerebrospinal Fluid Features in Patients With Coronavirus Disease 2019 and Neurological Manifestations: Correlation with Brain Magnetic Resonance Imaging Findings in 58 Patients
Authors: Lersy François , Benotmane Ilies , Helms Julie , Collange Olivier , Schenck Maleka , Brisset Jean-Christophe , Chammas Agathe , Willaume Thibault , Lefebvre Nicolas , Solis Morgane , Hansmann Yves , Fabacher Thibaut , Caillard Sophie , Mertes Paul Michel , Pottecher Julien , Schneider Francis , Meziani Ferhat , Fafi-Kremer Samira , Kremer Stéphane 
Published: November 2020

Abstract

Background
Neurological manifestations are common in patients with coronavirus disease 2019 (COVID-19), but little is known about pathophysiological mechanisms. In this single-center study, we examined neurological manifestations in 58 patients, including cerebrospinal fluid (CSF) analysis and neuroimaging findings.

Methods
The study included 58 patients with COVID-19 and neurological manifestations in whom severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) reverse-transcription polymerase chain reaction screening and on CSF analysis were performed. Clinical, laboratory, and brain magnetic resonance (MR) imaging data were retrospectively collected and analyzed.

Results
Patients were mostly men (66%), with a median age of 62 years. Encephalopathy was frequent (81%), followed by pyramidal dysfunction (16%), seizures (10%), and headaches (5%). CSF protein and albumin levels were increased in 38% and 23%, respectively. A total of 40% of patients displayed an elevated albumin quotient, suggesting impaired blood-brain barrier integrity. CSF-specific immunoglobulin G oligoclonal band was found in 5 patients (11%), suggesting an intrathecal synthesis of immunoglobulin G, and 26 patients (55%) presented identical oligoclonal bands in serum and CSF. Four patients (7%) had a positive CSF SARS-CoV-2 reverse-transcription polymerase chain reaction. Leptomeningeal enhancement was present on brain MR images in 20 patients (38%).

Conclusions
Brain MR imaging abnormalities, especially leptomeningeal enhancement, and increased inflammatory markers in CSF are frequent in patients with neurological manifestations related to COVID-19, whereas SARS-CoV-2 detection in CSF remained scanty.
Administrative Review Provisions in Chinese Investment Treaties: ‘Gilding the Lily’?
Authors: Chi Manjiao , Li Zongyao 
Published: November 2020

Abstract
Despite the popularization of investor-state arbitration (ISA), administrative review remains a helpful local remedy for investment-state dispute settlement (ISDS) in some states. China has a complicated and comprehensive legal system of administrative review. It has concluded a large number of international investment agreements (IIAs), and nearly half of them contain an administrative review provision. These provisions could be considered as an expropriation review mechanism, a standalone ISDS option, an ISA supporting measure or a pre-ISA requirement. Given that administrative review has legal and practical limits, and that China’s national law on dispute settlement and foreign investment governance keeps changing, the attractiveness and significance of administrative review for ISDS are diminishing. In China’s recent IIA-making, there appears an emerging trend of abandoning administrative review. In the long run, it remains to be seen how China will balance local remedies and ISA in IIA-making and foreign investment governance in the future.
Severe Acute Respiratory Syndrome-Coronavirus-2 Transmission in an Urban Community: The Role of Children and Household Contacts
Authors: Pitman-Hunt Chaya , Leja Jacqueline , Jiwani Zahra M , Rondot Dominique , Ang Jocelyn , Kannikeswaran Nirupama 
Published: November 2020

AbstractThis is a single-center US retrospective study of infection patterns among household sick contacts (HHSCs) of children with confirmed severe acute respiratory syndrome-coronavirus-2 infection in an urban setting. An HHSC was identified in fewer than half (42%) of the patients, and no child-to-adult transmission was identified.This is a single center US retrospective study of infection patterns among household sick contacts of children with confirmed SARS-CoV-2 infection. A household sick contact was identified in fewer than half (42%) of patients and no child-to-adult transmission was identified.
Fracture and embolization of an implantable venous access device in patient with atrial septal defect
Authors: Syltern Julie , Jørgensen Geir , Norstein Jarle , Augestad Knut Magne 
Published: November 2020

Abstract
Totally implantable venous access devices (TIVADs) are frequently used for the administration of chemotherapy or parenteral nutrition and have proved to be safe and improve patient quality of life during treatment. Catheter-related infections are the most common complication, whereas catheter fracture and embolization is rarely seen. We present a case of a 61-year-old cancer patient in which the TIVAD fractured and embolized to the patient’s left knee 18 months after its initial placement. An endovascular procedure with intraoperative imaging revealed that the catheter had embolized to the popliteal artery and was successfully removed, the only explanation being the presence of an atrial septal defect. A bubble echocardiogram was unsuccessful in confirming the diagnosis. The catheter fracture could be related to an intravascular procedure that was performed 18 months prior to remove fibrin sheaths as a cause of port malfunction, or it could be a case of pinch-off syndrome.
The Efficacy of the Stellate Ganglion Block as a Treatment Modality for Posttraumatic Stress Disorder Among Active Duty Combat Veterans: A Pilot Program Evaluation
Authors: Odosso Richard J , Petta Lorene 
Published: November 2020

ABSTRACTIntroductionThe lifetime prevalence for development of Posttraumatic Stress Disorder (PTSD) among military combat veterans has been estimated to be as high as 17%, or more than double the national average. The stellate ganglion block (SGB) has been proposed as a PTSD treatment modality that may potentially affect positive change in the attitude of a service member (SM) toward mental health treatment, lead to improved clinical outcomes, promote the likelihood of a return to a productive job performance, and decrease the economic burden of PTSD treatment on the Department of Defense.Materials and MethodsA pilot program to determine the efficacy of the SGB was conducted in a single, closed-unit of active duty combat veterans with a statistically high prevalence of PTSD-related symptoms associated with combat deployments. A retrospective project-level program evaluation was completed to assess the effectiveness of the treatment in reducing symptoms, the level of SM acceptance of the procedure, and the SM perceived response to the SGB treatment. The project-level program evaluation methodology was based on the suggestions of the W.K. Kellogg Foundation Evaluation Handbook. The primary design and context of the evaluation was viewed from an outcome evaluation perspective and format, with focus on both individual and system-level outcomes. The outcome evaluation addressed: 1) the measured outcomes and critical findings of the procedure during the pilot, 2) feasibility of and recommendation for replication of the pilot, and 3) the quality improvement potential of the SGB as part of a clinical pathway for the care and treatment of PTSD in SMs.ResultsProgram evaluation analysis demonstrated that the SGB exhibited success in both rate and level of response, reduced stigma relative to acceptance of the treatment, and SMs verbalized a high perceived value of the treatment. However, the effect of the procedure was shown to peak and plateau with a regression to baseline at roughly the 3-month timeframe, with a reciprocal decrease in perceived value.ConclusionThe most significant benefit of the SGB appears to be its ability to act as a gateway to treatment and to facilitate active participation and compliance by the SM during its “window” of efficacy. Additional research, including RCTs are required to continue to evaluate its efficacy relative to symptom-specific effectiveness, effectiveness of sequential procedures, and effectiveness when combined with other nonpharmaceutical treatment modalities. Moreover, the application of an accepted medical procedure as a mental health (MH) therapy with marked reduction in stigma, encourages pursuit of additional such treatment methods that reduce stigma and promote active SM participation in PTSD management.
A theoretical framework for BL Her stars – I. Effect of metallicity and convection parameters on period–luminosity and period–radius relations
Authors: Das Susmita , Kanbur Shashi M , Smolec Radoslaw , Bhardwaj Anupam , Singh Harinder P , Rejkuba Marina 
Published: November 2020

ABSTRACT
We present a new grid of convective BL Herculis models using the state-of-the-art 1D non-linear radial stellar pulsation tool mesa-rsp. We investigate the impact of metallicity and four sets of different convection parameters on multiwavelength properties. Non-linear models were computed for periods typical for BL Her stars, i.e. 1 ≤ P(d) ≤ 4 covering a wide range of input parameters – metallicity (−2.0 dex ≤ [Fe/H] ≤ 0.0 dex), stellar mass (0.5–0.8 M⊙), luminosity (50–300 L⊙), and effective temperature (full extent of the instability strip; in steps of 50 K). The total number of BL Her models with full-amplitude stable pulsations used in this study is 10 280 across the four sets of convection parameters. We obtain their multiband (UBVRIJHKLL′M) light curves and derive new theoretical period–luminosity (PL), period–Wesenheit (PW), and period–radius (PR) relations at mean light. We find that the models computed with radiative cooling show statistically similar slopes for PL, PW, and PR relations. Most empirical relations match well with the theoretical PL, PW, and PR relations from the BL Her models computed using the four sets of convection parameters. However, PL slopes of the models with radiative cooling provide a better match to empirical relations for BL Her stars in the Large Magellanic Cloud in the HKS bands. For each set of convection parameters, the effect of metallicity is significant in U and B bands and negligible in infrared bands, which is consistent with empirical results. No significant metallicity effects are seen in the PR relations.
The evolution of the size–mass relation at z = 1–3 derived from the complete Hubble Frontier Fields data set
Authors: Yang Lilan , Roberts-Borsani Guido , Treu Tommaso , Birrer Simon , Morishita Takahiro , Bradač Maruša 
Published: November 2020

ABSTRACT
We measure the size–mass relation and its evolution between redshifts 1 &lt; z &lt; 3, using galaxies lensed by six foreground Hubble Frontier Fields clusters. The power afforded by strong gravitation lensing allows us to observe galaxies with higher angular resolution beyond current facilities. We select a stellar mass limited sample and divide them into star-forming or quiescent classes based on their rest-frame UVJ colours from the ASTRODEEP catalogues. Source reconstruction is carried out with the recently released lenstruction software, which is built on the multipurpose gravitational lensing software lenstronomy. We derive the empirical relation between size and mass for the late-type galaxies with $M_{*}\gt 3\times 10^{9}\, \mathrm{M}_{\odot }$ at 1 &lt; z &lt; 2.5 and $M_{*}\gt 5\times 10^{9}\, \mathrm{M}_{\odot }$ at 2.5 &lt; z &lt; 3, and at a fixed stellar mass, we find galaxy sizes evolve as $R \rm _{eff} \propto (1+z)^{-1.05\pm 0.37}$. The intrinsic scatter is &lt;0.1 dex at z &lt; 1.5 but increases to ∼0.3 dex at higher redshift. The results are in good agreement with those obtained in blank fields. We evaluate the uncertainties associated with the choice of lens model by comparing size measurements using five different and publicly available models, finding the choice of lens model leads to a 3.7 per cent uncertainty of the median value, and ∼25 per cent scatter for individual galaxies. Our work demonstrates the use of strong lensing magnification to boost resolution does not introduce significant uncertainties in this kind of work, and paves the way for wholesale applications of the sophisticated lens reconstruction technique to higher redshifts and larger samples.
DIANA-miRGen v4: indexing promoters and regulators for more than 1500 microRNAs
Authors: Perdikopanis Nikos , Georgakilas Georgios K , Grigoriadis Dimitris , Pierros Vasilis , Kavakiotis Ioannis , Alexiou Panagiotis , Hatzigeorgiou Artemis 
Published: November 2020

Abstract
Deregulation of microRNA (miRNA) expression plays a critical role in the transition from a physiological to a pathological state. The accurate miRNA promoter identification in multiple cell types is a fundamental endeavor towards understanding and characterizing the underlying mechanisms of both physiological as well as pathological conditions. DIANA-miRGen v4 (www.microrna.gr/mirgenv4) provides cell type specific miRNA transcription start sites (TSSs) for over 1500 miRNAs retrieved from the analysis of &gt;1000 cap analysis of gene expression (CAGE) samples corresponding to 133 tissues, cell lines and primary cells available in FANTOM repository. MiRNA TSS locations were associated with transcription factor binding site (TFBSs) annotation, for &gt;280 TFs, derived from analyzing the majority of ENCODE ChIP-Seq datasets. For the first time, clusters of cell types having common miRNA TSSs are characterized and provided through a user friendly interface with multiple layers of customization. DIANA-miRGen v4 significantly improves our understanding of miRNA biogenesis regulation at the transcriptional level by providing a unique integration of high-quality annotations for hundreds of cell specific miRNA promoters with experimentally derived TFBSs.
APOBEC1 cytosine deaminase activity on single-stranded DNA is suppressed by replication protein A
Authors: Wong Lai , Vizeacoumar Frederick S , Vizeacoumar Franco J , Chelico Linda 
Published: November 2020

AbstractMany APOBEC cytidine deaminase members are known to induce ‘off-target’ cytidine deaminations in 5′TC motifs in genomic DNA that contribute to cancer evolution. In this report, we characterized APOBEC1, which is a possible cancer related APOBEC since APOBEC1 mRNA is highly expressed in certain types of tumors, such as lung adenocarcinoma. We found a low level of APOBEC1-induced DNA damage, as measured by γH2AX foci, in genomic DNA of a lung cancer cell line that correlated to its inability to compete in vitro with replication protein A (RPA) for ssDNA. This suggests that RPA can act as a defense against off-target deamination for some APOBEC enzymes. Overall, the data support the model that the ability of an APOBEC to compete with RPA can better predict genomic damage than combined analysis of mRNA expression levels in tumors and analysis of mutation signatures.