bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evolution of an indigenously developed climate-resilient potato variety “Kashmir” in Punjab, Pakistan
Authors: Hassan Syed I.U. , Khan Arifa , Iqbal Rana A. , Khan Rana A.R. , Sajid Muhammad N. , Javaid Tariq , Hussain Muhammad M. 
Published: December 2024

Climate change significantly challenges agriculture, impacting crop yields, water availability, pest attacks and soil health. Adaptation strategies like crop diversification, efficient mode of irrigation, and resilient crop varieties are the solution. In Pakistan, potato, a major food as well as cash crops, face climate change-related threats. “Kashmir” a high-yielding, early bulking, frost, and disease tolerant potato (Solanum tuberosum L.). variety was developed at the Potato Research Institute, Sahiwal, Pakistan. This is the result of a cross “FD 35-36 x SH-5” attempted at Potato Breeding Sub-Station, Murree in 2004. The nursery was raised during 2005-08, and evaluation trials were conducted from 2008-10 at Vegetable Research Institute, Faisalabad. The experimental design used in all trials was randomised Complete Block Design (RCBD). Varietal, Advanced Varietal, frost tolerance, National, and Zonal trials were conducted from 2012 to 2019 under different agro-climatic conditions using the same design. A split plot arrangement was implemented for early bulking studies conducted during 2016-17 at PRI, Sahiwal. Plant pathological studies were conducted under field conditions using CRD design at Kaghan during 2016-17, while agronomic studies were conducted at PRI, Sahiwal during 2016-17. Value addition studies were conducted at Post Harvest Research Institute, AARI, Faisalabad during 2020-21. In these studies, this variety exhibited a 53.6% higher yield than the commercial checks and took 100 days to maturity. Federal Seed Certification and Registration Department assessed its morphological features under the distinctness, Uniformity and Stability (DUS) trial in their field. This variety has been approved by Punjab Seed Council headed by the Minister of Agriculture.
Impact of feed availability on growth performance and amino acid utilization of cobia (Rachycentron canadum) at elevated temperature
Authors: Le Minh-Hoang , Espe Marit , Conceição Luís E. C. , Nguyen Minh V. , Yúfera Manuel , Engrola Sofia , Jordal Ann-Elise O. , Pham Hung Q. , Rønnestad Ivar 
Published: December 2024

IntroductionThis study explored the effects of increased water temperatures and limited feeding on the growth, feed utilization, and nutrient retention in juvenile cobia (Rachycentron canadum).MethodsJuvenile cobia, approximately 5.0 g in body weight, were distributed across two temperature conditions: 34°C, to mimic a global warming scenario, and 30°C, the current local summer average, as controls. The setup included eighteen 200-liter tanks, with 20 fish per tank. Within each temperature regime, triplicate tanks received one of three feeding levels (55%, 75%, and 95% of satiation as determined at 30°C) over a six-week period.ResultsThe findings indicated that cobia exhibited the poorest growth performance at the elevated temperature (34°C) and the lowest feeding level (55%). These conditions also correlated with the lowest protein efficiency ratio and feed intake. The feed conversion ratio worsened with increased temperature and feeding levels, while protein production values decreased at higher temperatures. A lower feeding level caused a lower total lipid retention but led to increased retention of whole body essential amino acids.DiscussionAfter six weeks, a 24-hour post-prandial analysis showed selective retention of some amino acids in muscle and plasma, but significantly higher retention in the liver at the higher temperature. Only a few amino acids' retention was influenced by feeding level. These results suggest that temperature and feeding levels alter the prioritization of amino acid metabolism and retention, as well as the utilization of energy and substrates across different organs of the cobia.
Efficacy evaluation of a minimally invasive surgical procedure (oblique lateral interbody fusion) for lumbar spinal tuberculosis—retrospective cohort study
Authors: Zheng Ke , Ni Zhihao , Han Guosong , Shan Tao , Xu Bin 
Published: December 2024

ObjectiveIn the current study, to demonstrate the advantages of oblique lateral interbody fusion (OLIF), we focused on the therapeutics for lumbar spinal tuberculosis with the comparison of three treatments, including anterior approach, posterior approach, and OLIF combined with posterior percutaneous pedicle screw fixation.MethodsThis study included patients with lumbar spinal tuberculosis from July 2015 to June 2018. We divided these patients into three groups: 35 patients underwent an anterior-only approach (Group A), 36 patients underwent a posterior-only approach (Group B), and 31 patients underwent OLIF combined with posterior percutaneous pedicle screw fixation (Group C). Operation time, blood loss, hospital stays, the visual analog scale (VAS) and the Oswestry disability index (ODI), ASIA grade, the erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), and Cobb angle were used to evaluate the surgical approaches.ResultsA total of 102 patients joined this study of three therapeutic groups. The mean hospital stays, the mean operative time, and surgical blood loss of the three groups of patients were (14.40 ± 2.6, 14.00 ± 2.51, and 9.39 ± 1.86) days, (177.23 ± 13.23, 198.00 ± 16.75, and 150.39 ± 14.28) minutes, and (307.43 ± 21.91, 406.67 ± 27.02, and 105.97 ± 18.90) mL, respectively. VAS and ODI of all patients significantly improved 1 week after surgery (P &lt; 0.05). As all patients received regular anti-tuberculosis treatment before and after surgery, ESR and CRP indicators maintained at normal levels 1 week after surgery. The Cobb angle was significantly corrected 1 week after surgery (P &lt; 0.05). Eight patients had postoperative complications, and all of them recovered after active treatment.ConclusionOLIF combined with posterior percutaneous pedicle screw fixation has the advantages of less surgical trauma and faster postoperative recovery, although all three surgical approaches can achieve satisfactory clinical results.
Deciphering pathogenicity and virulence of the first Staphylococcus debuckii isolate from diabetic foot osteomyelitis
Authors: Abi Najem Cynthia N. , Magnan Chloé , Plumet Lucile , Ahmad-Mansour Nour , Pouget Cassandra , Morsli Madjid , Pantel Alix , Kissa Karima , Sotto Albert , Lavigne Jean-Philippe , Molle Virginie 
Published: December 2024

IntroductionThis study identifies Staphylococcus debuckii as a new coagulase-negative staphylococcal species isolated from diabetic foot osteomyelitis (DFOM) and provides an in-depth analysis of its pathogenic and virulence profile, as well as demonstrating its potential to cause infection.MethodsThe S. debuckii NSD001 strain was examined for its planktonic growth, biofilm production, and phagocytosis rates in murine macrophages compared to S. aureus NSA739. Additionally, persistence and replication within human osteoblasts were investigated, while the zebrafish embryo model was employed to assess virulence. Genomic sequencing and bioinformatic analysis were also conducted to identify genes associated with virulent potential.Results and DiscussionS. debuckii NSD001 exhibited robust planktonic growth and significant biofilm production, highlighting its capacity to initiate and maintain an infection, and demonstrated similar rates of phagocytosis as S. aureus NSA739 in murine macrophages, suggesting a mechanism for evading initial host defenses. The strain persisted and replicated within human osteoblasts, indicative of a strategy for intracellular survival and facilitation of chronic osteomyelitis. The zebrafish embryo model revealed a slower, yet fatal, virulence profile for S. debuckii NSD001 compared to the rapid lethality induced by S. aureus NSA739. Genomic sequencing and bioinformatic analysis uncovered various genes corroborating its virulence. S. debuckii NSD001 poses a significant concern in DFOM due to its ability to form biofilms and survive within host cells, presenting challenges for current treatment strategies. This underscores the need for updated clinical protocols and increased awareness among healthcare professionals to effectively manage infections caused by this emerging pathogen.
Clinical characteristics of adrenal crisis in 371 adult patients with glucocorticoid-induced adrenal insufficiency
Authors: Qiu Ying , Luo Ying , Geng Xinqian , Li Yujian , Feng Yunhua , Yang Ying 
Published: December 2024

BackgroundGlucocorticoid-induced adrenal insufficiency (GIAI) is a hypothalamic-pituitary-adrenal (HPA) axis dysfunction caused by long-term use of exogenous steroids. Adrenal crisis (AC) is an acute complication of GIAI and one of the reasons for the increased risk of death. This study aims to analyze the clinical characteristics of GIAI patients with AC and explore the related risk factors.MethodsClinical data of adult GIAI patients treated at our hospital between January 1, 2014, and December 31, 2023 were included. The demographic characteristics, clinical characteristics, laboratory tests and comorbidities of the patients were collected. Univariate and multivariate regression analyses were used to explore the variables related to the occurrence of AC, and prediction models were constructed.Results51 patients (13.75%) developed AC during hospitalization. Mortality was significantly higher in patients with AC than in those without AC. Multivariate logistic regression analysis showed that infection, psychiatric symptoms, serum sodium, albumin, neutrophil-lymphocyte ratio (NLR) and eosinophil-lymphocyte ratio (ELR) were independent risk factors for AC. Among the prediction models constructed by machine learning algorithms, logistic regression model had the best prediction effect.ConclusionThis study investigated the clinical characteristics of AC in GIAI patients. NLR and ELR may be effective predictors of AC in GIAI patients, and combined with other clinically significant indicators, an effective prediction model was constructed. Logistic regression model had the best performance in predicting AC in GIAI patients.
Evaluating the therapeutic impact of Compound Polymyxin B Ointment on postoperative wound healing in patients with perianal abscesses
Authors: Li Yan-Zhu , Zhou Fu-Rong , Chen Xiao-Juan , Liu Yong-Gan 
Published: December 2024

IntroductionPerianal abscesses pose a considerable obstacle in the realm of postoperative wound treatment owing to their elevated susceptibility to infection and associated consequences. Polymyxin B Ointment, a compound renowned for its antibacterial qualities, has the potential to provide therapeutic advantages by promoting wound healing and mitigating postoperative problems.MethodsOur institution conducted a thorough retrospective analysis spanning from December 2020 to December 2023 to assess the effectiveness of Compound Polymyxin B Ointment in the management of surgical wounds in patients diagnosed with perianal abscesses. The research encompassed a cohort of 100 individuals, who were classified into two groups: a control group that received conventional postoperative care, and an observation group that received supplementary treatment with Compound Polymyxin B Ointment. The evaluation of clinical outcomes involved measuring wound healing effectiveness, pain intensity using the Visual Analogue Scale (VAS), tissue swelling, exudation, necrotic tissue shedding time, duration of hospital stays, and rate of reduction in wound area.ResultsThe group that received Compound Polymyxin B Ointment had significant enhancements in wound healing, as seen by a noteworthy 46% of participants completing complete healing, in contrast to the control group’s 32%. The VAS was used to quantify pain levels, and the observation group reported a substantial reduction of almost 50% in ratings. Furthermore, this cohort exhibited a 45% decrease in edema and a 50% decline in exudation rates, in addition to a 50% acceleration in the shedding of necrotic tissue. The duration of the hospital stay was reduced by 40%, and the reduction in wound area was 18% higher, suggesting a more effective healing process. In addition, it is worth noting that the observation group had a lower incidence of problems, so underscoring the effectiveness of the ointment in facilitating wound healing and mitigating postoperative difficulties.DiscussionThe utilization of Compound Polymyxin B Ointment as an adjuvant measure in the surgical treatment of perianal abscesses has been found to have a substantial positive impact on wound healing, pain relief, and complication reduction. This finding provides evidence for the possibility of the ointment as a helpful inclusion in post-surgical wound care procedures among this specific group of patients.
The prevalence of obstructive sleep apnea-hypopnea syndrome in patients with multiple sclerosis: a systematic review and meta-analysis
Authors: Li Peipei , Liu Jiaqi , Yang Jianying , He Jie , Jiang Jiaqing 
Published: December 2024

ObjectiveObstructive sleep apnea-hypopnea syndrome is the most common respiratory disorder in patients with multiple sclerosis. The purpose of this meta-analysis was to evaluate the prevalence of OSAHS in MS patients and to analyze their sleep monitoring indicators of patients.MethodsOnline databases such as PubMed, EMBASE, Web of Science, CNKI, and WanFang were used to review the Chinese and English literature about OSAHS in MS patients in detail. Two researchers analyzed the Quality of included studies based on the Quality Assessment of Diagnostic Accuracy Studies. The prevalence and sleep monitoring data were analyzed using STATA 11.0 software. Based on the I2 values, pooled analyses were performed using either random (I2 &gt; 50%) or fixed-effect models (I2 ≤ 50%).ResultsFourteen articles were selected for the final analysis. Our study shows that different diagnosis methods of OSAHS lead to different incidences. When the screening method was PSG, the incidence of OSAHS in MS patients was 36%; when the method was STOP-BANG, the incidence of OSAHS in MS patients was 26%; when the method was Berlin questionnaire, the incidence of OSAHS in MS patients was 30%. We performed subgroup analyses based on race, age, OSAHS severity, and BMI of patients with MS. The results suggested that the incidence of OSAHS was different in different subgroups of MS patients. In addition, we found that patients with MS generally had poorer sleep monitoring indicators.ConclusionThe current literature shows that the incidence of OSAHS is higher in MS patients. MS may affect the progression of OSAHS.Systematic Review Registrationhttps://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=551500, CRD42024551500.
Small intestinal bacterial overgrowth and metabolic dysfunction-associated steatotic liver disease
Authors: Wang Ziteng , Tan Wentao , Huang Jiali , Li Qian , Wang Jing , Su Hui , Guo Chunmei , Liu Hong 
Published: December 2024

Small intestinal bacterial overgrowth (SIBO), characterized by alterations in both the type and quantity of bacteria in the small intestine, leads to impaired intestinal digestion and absorption that can cause a range of clinical symptoms. Recent studies have identified significant changes in the composition of the small intestinal microbiota and metabolomic profiles of patients with metabolic dysfunction-associated steatotic liver disease (MASLD). This study systematically reviewed and synthesized the available data to explore the association between SIBO and MASLD. Comprehensive literature searches of the Embase, PubMed, Web of Science, Ovid, and Cochrane databases were conducted. Article quality screening was performed using the Newcastle-Ottawa Quality Assessment Scale. Cross-sectional, cohort, and case–control studies were included. A total of 7,200 articles were initially screened, of which 14 were ultimately included for analysis. Individuals with SIBO in both the MASLD and non-MASLD groups were extracted and a chi-square test was performed to calculate the odds ratio (OR) and 95% confidence interval (CI). The I2 index was used to measure heterogeneity. For heterogeneity &gt;50%, a random effects model was used. There was a clear association between SIBO and MASLD (OR = 3.09; 95% CI 2.09–4.59, I2 = 66%, p &lt; 0.0001). Subgroup analyses by MASLD stage showed that the probability of SIBO positivity increased with MASLD lesion severity. After stratifying by the diagnostic methods for SIBO and MASLD, the meta-analysis results suggest a reduction in inter-group heterogeneity. For the MASLD subgroup diagnosed via liver biopsy, the OR was 4.89. A subgroup analysis of four studies that included intestinal permeability testing revealed an OR of 3.86 (95% CI: 1.80–8.28, I2 = 9%, p = 0.0005). A meta-regression analyses revealed that both race and regional development level significantly influenced the relationship between SIBO and MASLD (p = 0.010, p = 0.047). In conclusion, this meta-analyses provides strong evidence that SIBO may contribute to the development and progression of MASLD. The strongest associations were observed between lactulose breath testing, gut microbiota culture, liver biopsy diagnosis of MASLD, and SIBO detected through intestinal permeability testing. The primary sources of heterogeneity are race and developed regions.Systematic Review Registrationhttps://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=427040.
Interstitial lung diseases with concomitant lung cancer: a data mining approach revealing a complex condition with gender- and immune-associated specific implications
Authors: Perrotta Fabio , Lacedonia Donato , D’Agnano Vito , Bianco Andrea , Scioscia Giulia , Tondo Pasquale , Foschino Barbaro Maria Pia , Mariani Francesca , Lettieri Sara , Del Frate Lucia , Mancinelli Silvia , Piloni Davide , Oggionni Tiberio , Bortolotto Chandra , Carrozzi Laura , Cerveri Isa , Guido Corsico Angelo , Stella Giulia Maria 
Published: December 2024

BackgroundInterstitial lung diseases (ILDs) comprise a family of heterogeneous entities, primarily characterised by chronic scarring of the lung parenchyma. Among ILDs, idiopathic pulmonary fibrosis (IPF) is the most common idiopathic interstitial pneumonitis, associated with progressive functional decline leading to respiratory failure, a high symptom burden, and mortality. Notably, the incidence of lung cancer (LC) in patients already affected by ILDs—mainly IPF—is significantly higher than in the general population. Moreover, these cases are often neglected and deprived of active oncologic treatments.MethodsWe here aim to identify variables predictive of outcome (mortality) in a multicentre retrospective cohort of ILD associated with lung cancer, collected from 2018 to the end of 2023. Overall, 73 cases were identified, and exhaustive clinicopathologic data were available for 55 patients. Among them, 42 had IPF. The entire dataset was then analysed by using the JMP partition algorithm (JMP-Statistical Discoveries, from SAS), which can choose the optimum splits from many possible trees, making it a powerful modelling and data discovery tool.ResultsThe average age at lung cancer diagnosis was 71.4 years, whereas the average age at IPF diagnosis was 69.5 years. The average Charlson Comorbidity Index was 4.6. Female patients constituted 28.3% (15) of the evaluated cases. The most frequent tumour histotype was adenocarcinoma (45.2%), and in more than 60% of the cases (67.9%), cancer was diagnosed at an early stage (TNM I–II–IIIA). A significant gender difference emerges regarding the overall patient survival, and quite unexpectedly, surgical approach to IPF-associated LC and the detection of serum autoantibodies are among the strongest outcome predictors.ConclusionsThe analysis performed is descriptive and successfully identifies key features of this specific and rare cancer population. IPF-associated LC emerges as a unique malignant disease defined by specific gender and histopathologic clinical and molecular parameters, which might benefit from active treatments.
Obinutuzumab-induced acute thrombocytopenia: a case report and literature review
Authors: Dou Xuelin , Li Kongyang , Lu Jin 
Published: December 2024

BackgroundObinutuzumab, a humanized type II anti-CD20 monoclonal antibody, is widely used in the treatment of B-cell lymphomas. Thrombocytopenia typically occurs 1 to 2 weeks after administration. In rare cases, obinutuzumab can induce severe acute thrombocytopenia within days of infusion, a condition known as “obinutuzumab-induced acute thrombocytopenia (OIAT).” Rituximab, a chimeric type I anti-CD20 monoclonal antibody, is also known to cause “rituximab-induced acute thrombocytopenia (RIAT).” This report presents a case of OIAT, with subsequent treatment switched to rituximab, which did not result in thrombocytopenia recurrence.Case PresentationA 38-year-old female patient with a 2-year history of lymphadenopathy was diagnosed with follicular lymphoma (Grade I-II). She was treated with obinutuzumab combined with bendamustine. Following the first administration of obinutuzumab, her platelet count dropped to 37×10⁹/L within 2 days and further declined to 27×10⁹/L on the fourth day without bleeding symptoms. The platelet count recovered by day 8. After a second obinutuzumab infusion, the platelet count again dropped to 15×10⁹/L within 1 day. Platelet transfusion was effective, and the count eventually recovered to 95×10⁹/L by day 29. No further acute thrombocytopenia occurred after switching to rituximab.ConclusionOIAT is a rare but serious adverse effect of obinutuzumab. This case highlights the importance of early recognition and monitoring of platelet counts in patients receiving obinutuzumab. The findings in our case, along with those in the literature, suggest that switching to rituximab or extending the interval before obinutuzumab re-administration can reduce the risk of recurrent thrombocytopenia. Further research is needed to elucidate the underlying mechanisms and establish treatment guidelines for OIAT.
Pyridostigmine as a therapeutic option for pediatric gastrointestinal dysmotilities in ATR-X syndrome. Case report and literature review
Authors: Comisi F. F. , Soddu C. , Corpino M. , Marica M. , Cacace R. , Foiadelli T. , Savasta S. 
Published: December 2024

BackgroundAlpha-thalassemia X-linked intellectual disability (ATR-X) syndrome, is a rare genetic disorder, caused by mutations in the ATRX gene. Clinical manifestations include typical facial dysmorphisms, mild-to-severe intellectual disability, hypotonia, genital anomalies, significant gastrointestinal (GI) complications, such as abdominal distension, chronic constipation, feeding difficulties, gastroesophageal reflux, and mild-to-moderate anemia secondary to alpha-thalassemia.Case presentationWe report a patient with ATR-X syndrome suffering from gastrointestinal dysmotility and highlight the beneficial effects of pyridostigmine. Knowledge about the role and appropriate dosage of pyridostigmine in GI motility disorders is limited. To date, only nine pediatric cases involving pyridostigmine for GI dysmotility have been reported.ConclusionsConsidering current understanding about the treatment of gastrointestinal complications in patients with genetic syndromes, this case provides new insights into management of these complex clinical presentations.