bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dual Bioelectrical Impedance Analysis Correlates Well with Computed Tomography to Estimate Visceral Fat in Grade I-II Obese Mexican Patients
Authors: Muñoz Ernesto G. Cardona , Páez Fernando Grover , Becerra Carlos Gerardo Ramos , Sutto Sylvia E. Totsuka , Sánchez Guillermo Adrián Alanis , Corral Jesús Ricardo García , Aguilar Catherine A. García , Iantchoulev Assen Ognianov , Bernal José Rafael Villafán , Barajas Ana Leticia Milanés 
Published: February 2020

Objective: To correlate visceral fat using bioelectrical impedance analysis (Dual BIA) with computed tomography (CT) in Grade I-II Obese Mexican Patients.
Research Design and Methods: We recruited 30 Mexican patients from the western part of the country (15 men and 15 women, aged 50-65 years) with class I-II obesity (body mass index [BMI] 30-39.9 Kg/m2).
To evaluate the IAFA by Dual BIA comparing with CT.The dual BIA instrument consists of a bioelectric impedance component that measures the physical size of the abdomen. The truncal impedance is measured by applying electrical currents between the upper and lower limb leads and reading the voltage from the electrodes around the abdominal circumference. The surface impedance is measured by applying and measuring the voltage from the abdominal circumference electrodes. We took computed tomography (CT) at the umbilical level and calculated the visceral fat area (VFA).BMI was calculated as body weight (in kilograms) divided by the squared height (in meters). Waist circumference (WC) was measured at the level of the umbilicus to the nearest 0.1 cm in the standing position at the end of expiration.
Results: We observed a strong correlation between both methods used to calculate IAFA (BIA and CT; Pearson’s r2 =0.659, p < 0.001). Though both groups had significantly correlating values, the female group had a clearly stronger correlation than the male group (Pearson’s r2 = 0.667 vs. 0.501). This method exhibited a high level of reproducibility and a strong correlation with the CT results.
Financial Reporting Practices in the Textile Manufacturing Sectors of Bangladesh
Authors: Rouf Md. Abdur , Hasan Md. Salim , Ahmed Alim Al Ayub 
Published: February 2020

The aim of this study is to examine the factors that influence companies to disclose voluntary information in their annual reports of Textile Manufacturing Companies in Bangladesh. The study is used ordinary least squares (OLS) regression model to examine the relationship between dependent variable and independent variables. The factors are proportion of independent non-executive directors (INDs), board size, board audit committee, ownership structure, Profitability and firm size. The extent of voluntary disclosure level is measured by using 68 items of information. Data have been taken from annual reports of 21 listed Textile Manufacturing Companies in DSE of Bangladesh-2010. The result shows a positive association between board size and voluntary disclosure and also total assets with voluntary disclosure. In contrast, the extent of voluntary disclosure is negatively related to the ownership structure.
Management of distal femur aneurysmal bone cyst in an adult by extended curettage with bone substitute and internal fixation: an illustrative case report
Authors: Yogadeepan D. , Rajappa Srinivasan , . Raghavendran 
Published: February 2020

<p class="abstract">Aneurysmal bone cysts (ABCs) are benign osteolytic vascular neoplasm characterised by several sponge like blood filled or serum filled non endothelialized spaces of various diameter. ABCs can present a diagnostic challenge, as they share several histological and radiographic characteristics with other aggressive lesions like giant cell tumors and malignant telangiectatic osteosarcomas. In this article, we present a case of 34-year gentleman with left distal femur aneurysmal bone cyst treated by extended curettage augmented with bone substitute and internal fixation. In ABC management, curettage is the treatment of choice inspite of high recurrence rate. This is because of early return to functional activities and less morbidity. En bloc excision results in a lower recurrence rate but more extensive reconstructive surgery is needed which is associated with higher morbidity</p>
The Claim to Correctness and Moral Constraints on Law: a Critic to the Separability Thesis
Authors: Nava Tovar Alejandro 
Published: February 2020

O presente artigo explica a pretensão de correção e os seus efeitos limitadores sobre o Direito, de acordo com a teoria do direito de Robert Alexy. Para apresentar isso, na primeira seção é apresentado o conceito e a natureza normativa da pretensão de correção na visão de Robert Alexy. Na segunda seção são apresentadas as respostas de Eugenio Bulygin e Joseph Raz à pretensão de correção. Na terceira seção é conectada a pretensão de correção à ideia de lmitações morais para, no final, na quarta seção, ser apresentadas considerações sobre a pretensão de correção, em especial, proposta para reduzir o grau de arbitrariedade e injustiça no direito.
Miejsce Ukrainy w stosunkach rosyjsko-amerykańskich po 2014 roku
Authors: Legucka Agnieszka 
Published: February 2020

Celem niniejszego artykułu jest odpowiedź na pytanie, jakie miejsce zajmuje Ukraina w stosunkach między Rosją i USA. W pierwszej części podjęto w nim kwestię znaczenia Ukrainy dla interesów narodowych obu międzynarodowych rywali. Przyjęto perspektywę czasową po 2014 r. (od aneksji Krymu) do 2017 r. (wyborów prezydenckich w USA). W drugiej części ukazano ewolucję amerykańskiego podejścia do bezpieczeństwa Europy Wschodniej, w tym kwestię dozbrajania państwa ukraińskiego walczącego z rosyjskimi oddziałami. Trzecią część poświęcono konsekwencjom wydarzeń w Ukrainie, zarówno Euromajdanu, jak i wojny na wschodzie Ukrainy dla relacji USA i Rosji. W konkluzjach stwierdzono więc, że Ukraina stała się wyznacznikiem relacji między USA i Rosją, a rok 2014 stał się punktem zwrotnym w strategii USA wobec NATO. Doszło do wzmocnieniu wschodniej flanki sojuszu, a także nałożenia amerykańskich sankcji na Rosję.
VII Rotation of Polish Military Contingent in Afghanistan from the Perspective of Polish Soldiers
Authors: Zaręba Agnieszka 
Published: February 2020

VII zmiana polskiego kontyngentu wojskowego w Afganistanie z perspektywy polskich żołnierzy
VII zmiana w Afganistanie jest określana jako jedna z najtrudniejszych operacji stabilizacyjnych, przede wszystkim ze względu na zadania jakie były stawiane kontyngentowi (przede wszystkim ze względu na zapewnienie bezpieczeństwa w trakcie wyborów parlamentarnych), oraz warunki polityczno-społeczne w tamtym czasie. Celem niniejszej analizy jest zaprezentowanie VII zmiany w Afganistanie z perspektywy żołnierza. Analiza nie jest w żaden sposób odzwierciedleniem oficjalnych relacji z operacji pokojowej. Pokazuje codzienne obowiązki żołnierza, trud służby za granicą, relacje z Afgańczykami oraz wymagania, z jakimi muszą sobie radzić szeregowi żołnierze. Jest to analiza case study, uzupełniona źródłami o podobnym charakterze.
Метацеркарии трематод карповых рыб р. Северский донец и его притоков
Authors: Присный Ю.А. , Кононова М.И. , Винаков Д.В. 
Published: February 2020

У карповых рыб из р. Северский Донец и его притоков – рр. Везелка, Разумная и Нежеголь на сегодняшний день отмечено 16 видов метацеркарий трематод из 7-ми семейств, среди которых имеются виды, являющиеся потенциально опасными эпизоотологически (представители рр. Diplostomum, Ichthyocotylurus, а также Apophallus muehlingi и Posthodiplostomum cuticola) и эпидемиологически (Pseudamphistomum truncatum, Metagonimus yokogawai и Paracoenogonimus ovatus). Промежуточными хозяевами большей части отмеченных трематод являются широко распространенные двустворчатые и брюхоногие моллюски из рр. Anodonta и Unio, Lymnaea, Lithoglyphus, Bithynia, Planorbis, Viviparus, Valvata и Physa. Представители всех перечисленных родов отмечены в Белгородской области, в том числе и специфичные для отдельных видов трематод промежуточные хозяева. Для таких опасных в эпидемиологическом плане видов, как Metagonimus yokogawai и Pseudamphistomum truncatum в исследуемом регионе следует провести целенаправленные исследования по установлению промежуточных хозяев. Дополнительными хозяевами, то есть хозяевами собственно метацеркарий, для всех отмеченных видов являются многие карповые рыбы, а зачастую и рыбы других семейств. Поэтому отмеченные виды метацеркарий будут, скорее всего, в том или ином количестве встречаться у других видов рыб, которые еще не были охвачены исследованиями в регионе. Apophallus muehlingi и Posthodiplostomum cuticola являются возбудителями «черно-пятнистой болезни» карповых и могут вызывать гибель молоди рыб, а представители диплостомид и ихтиокотилурид могут провоцировать развитие очагов трематодозов. Среди 16-ти отмеченных видов метацеркарий только один вид трематод поражает во взрослом состоянии рыб – это Rhipidocotyle campanula. Остальные же виды являются преимущественно паразитами птиц, таких как цапли, чайки, поганки, кваквы, выпи и вороны, которые являются обычными для территории области.
Численность популяции жука-оленя lucanus cervus (l.) Лесного массива в Воронежской области, рассчитанная по пищевым остаткам сорокопута-жулана lanius collurio (l.)
Authors: Голуб В.Б. , Шестопалов А.Г. , Масалыкин А.И. 
Published: February 2020

На основе результатов подсчёта количества свежих фрагментов тела жуков-оленей Lucanus cervus (L.) в июне 2018 года, представляющих собой пищевые остатки сорокопута-жулана Lanius collurio (L.) и с учетом площади гнездовой территории жулана, рассчитаны примерная плотность популяции и примерная численность всей популяции жука-оленя дубравы площадью 5 тыс. га (Воронежская область).
Дополнения к фауне и распространению цикадообразных – cicadomorpha (hemiptera: homoptera) юга Среднерусской возвышенности
Authors: Присный А.В. 
Published: February 2020

Белгородская область расположена на юго-западе Среднерусской возвышенности. В геоморфологическом и биогеографическом планах она очень неоднородна. Её западные и северо-западные районы лежат в провинции Приднепровской низменности лесостепи (бассейн р. Днепр). Юго-восточные районы, по особенностям флористических и фаунистических комплексов, относят к Нижне-Донской провинции степной зоны. Основная часть рассматриваемой территории попадает в выделяемую провинцию Среднерусской возвышенности лесостепи (типичный и кальцефитный варианты). Одновременно регион характеризуется очень высоким уровнем хозяйственной освоенности и антропогенной трансформации ландшафта. Цикадообразные – один из таксонов насекомых, присутствующих во всех естественных и искусственных наземных биоценозах. Несколько их видов являются переносчиками вирусов – возбудителей заболеваний культурных растений, а некоторые – индикаторами состояния естественных сообществ. Целенаправленное изучение фауны цикадообразных Белгородской области насчитывает около 100 лет. Подавляющее большинство сборов проведено на юго-западе и юго-востоке области. Приводимые в статье сведения о цикадообразных региона – результат выборочной обработки общих энтомологических сборов автора за период с 1977 г. по 2014 г. Частично они были опубликованы в открытой печати с 2002 г. по 2014 г. В отличие от прежних публикаций автора, здесь приводится аннотированный список видов, поставленных в коллекцию кафедры биологии НИУ «БелГУ». Общий список цикадообразных, зарегистрированных в регионе, включает 209 видов. 135 видов, известные нам по публикациям, представляют несколько групп: 39 – лесные, луговые и болотные, отмеченные в основном до 1949 (1956) гг. на юго-западе области; 29 – луговые виды сем. Cicadellidae и Cixiidae, собранные как на юго-западе, так и на юго-востоке области до 2000 г.; 67 видов – степные, лугово-степные, луговые и лесные – из окрестностей г. Валуйки (в их числе 21 вид отмечен только до 1913 г). Лишь три вида приводятся разными авторами из других районов области. Из 74-х видов, собранных нами и большей частью уже отмеченных в наших публикациях, 16 приводятся впервые для территории Белгородской области (D. venosus, D. bohemani, E. pteridis, H. obsoletus, K. kirgisorum, L. striatellus, L. aurita, M. inermis, N. guttulatus, O. russeola, P. paludosus, P. nigra, R. quinquecostatus, St. fuscovittatus, T. atrata, T. depressa), 9 – для Белгородской области и 1–3-х смежных областей (M. fuscula, S. dahlbomi, M. mayri, D. baeri, Sc. montana, P. punctifrons, T. griseola, B. laetus, Tsh. eugeniae) и 4 – для приграничных районов Луганской или Харьковской областей (A. astrachanicus, C. affinis, N. albipennis, T. fusca). Кратко обсуждаются возможные причины объема и направленности изменений фаунистического списка цикадообразных региона.
In Vitro Propagation of Lilium longiflorum Bulbs Using NAA and BAP Plant Growth Regulator Treatment
Authors: Deswiniyanti Ni Wayan , Dwipayani Lestari Ni Kadek 
Published: February 2020

Lily (Lilium longiflorum) is a perennial herbaceous plant with white trumpet-shaped flowers, fragrant and bulbous. In vitro culture through bulbs is one of way propagation of lily plants, but it requires a long time and only produces limited plants. In vitro propagation is a very promising technique for plant propagation because it can produce a lot of plant seeds in a short time. Bulbs are one of the fastest explants for growing shoots in lilies, but it is not known for certain which cuts of explants from bulb scales are best for multiplying in vitro. This study aims to determine the effect of lily bulb explants and the concentration of NAA and BAP growth regulators on the growth of lily bulb explants. The best results were obtained on the base and middle cuts explant of bulb scales compared to the tip cuts explant ones. The best results of the growing percentage, the number of shoots and the best growing time are shown in the combination treatment of growth regulator 1 mg L−1 NAA and 1 mg L−1 BAP. The optimum results on the number of micro bulbs were found in the treatment of growth regulators 0.5 mg L−1 NAA and 1 mg L−1 BAP. The best results of the average time formed micro bulb was in the treatment of 1 mg L−1 NAA and BAP with middle explant cuts, and treatment concentrations of 0.5 mg L−1 NAA and BAP in the base explant section. The base and middle bulb explants are able to regenerate or grow higher shoots. This is caused by the presence of endogenous natural auxin and the spread of auxin in plant parts not in the same amount. Therefore when added to the exogenous growth regulator such as auxin or cytokines to culture media will further trigger the formation of micro tubers more quickly,. It can increase the concentration of endogenous growth regulators in cells, help growing process and developing tissue.

Keywords: Bulb, lily, micro bulbs, in vitro, shoots
Specific Primer for Human Bcl-2-Protein Expression Analysis
Authors: Jannah Patty Dessy Nurul , Ayu Safitri Tazkia , Saraswati Henny 
Published: February 2020

Bcl-2 protein (B-cell lymphoma 2) is one of the proteins in the Bcl-2 protein family that are anti apoptotic and encoded by the BCL-2 gene. This protein controls the permeability of the outer membrane of the mitochondria, which plays a role in the release of cytochrome c and several other proteins that contribute to cell death. Bcl-2 protein plays a role in regulating cell death and regulating the balance between new and old cells. Several studies showed that changes in the structure and function of Bcl-2 play a role in cell apoptosis disorders, producing cancer cells. High expression of Bcl-2 protein is seen in lung, breast, lymph node and brain cancers. Analysis of Bcl-2 protein expression at the molecular level in cancer cell is needed to determine the expression level of this protein. This study aims to produce the Bcl-2 primer for qPCR method that specifically identifies the target gene, and can be used to see the BCL-2 gene expression level in cells. A BCL-2 sequence was obtained from the National Center for Biotechnology Information (NCBI). The next process is further analysis with Primer-BLAST (NCBI). Some primer candidates are produced and undergo another selection based on several criteria. Finally, one pair of potential primers was obtained for the reaction of qPCR to BCL-2. Primer specificity was tested with Basic Local Alignment Tools (BLAST) tools and result in specific primer for human BCL-2 gene. Annealing temperature optimization shows that the primer can react optimally at 59∘C.

Keywords: Bcl-2 protein, BCL-2 gene, qPCR, primer, gene expression, apoptosis
Computational Design of Ancestral and Consensus Asian Dengue Envelope Protein for Vaccine Candidate
Authors: Azhari Kemal Rahmat , Ivan Jeremias , Lili Sandjaja Eric Bernardus , Putra Santosa Audi 
Published: February 2020

Dengue is a mosquito-borne viral disease of which incidence has rapidly increased in the last few years. Despite the recent development of a licensed dengue vaccine, safer and more efficacious dengue vaccine still needs to be developed. Dengue virus has four antigenically and genetically distinct serotypes. Ancestral sequence reconstruction (ASR) and consensus sequence (CS) might be able to overcome antigenic distinction between those four serotypes. Envelope (E) protein is responsible for a wide range of dengue virus biological activities. Domain III of the E protein (EDIII) plays a role in receptor binding for viral entry and inducing protective immunity against the dengue virus. We utilised bioinformatics software to computationally design ancestral and consensus sequences of Asian dengue E protein. E protein sequences of 987 DENV strains and 5 outgroups were retrieved from GenBank. We constructed ancestral and consensus sequences for each serotype. For ASR, ancestral sequences were gradually designed to construct ancestral sequence for all serotypes using MEGA X. For CS, all four consensus sequences were directly used to construct consensus sequence for all serotypes using UGENE 1.32. Phylogenetic tree consisting existing dengue sequences as well as ancestral and consensus sequences were visualised using FigTree 1.4.4. All ancestral and consensus sequences were analysed for conserved motifs, especially in domain III region. ASR sequences were closer to the centre of phylogenetic tree branches while consensus sequences were located among natural isolates. Further CD4 T cell immunogenicity prediction on domain III (EDIII) showed that both ASR and consensus EDIII have the two-highest combined immunogenicity scores. These sequences are potential for further in vitro and in vivo studies as dengue vaccine candidate.

Keywords: ancestral sequence, consensus, dengue, envelope protein, vaccine
The Evolution Study Of 6-Cysteine Family Member Protein of Plasmodium sp. As a Potential Drug Candidate Against Malaria Infection
Authors: Nathanael Ramanto Kevin , Nurdiansyah Rizky , Jessica Priscilla 
Published: February 2020

The increase of current antimalarial drug resistance was reported and lead to the greatest threats to malaria control; therefore, new methods should be applied to encounter this problem. Although the protein evolution study of Plasmodium may contain valuable information in finding a new antimalarial drug candidate, the cross-species antimalaria drug cannot be made because there is no sufficient information regarding the protein evolution between human-infecting and non- infectious Plasmodium. In this study, data mining from PlasmoDB discovers several proteins shared by Plasmodium where some of them include in a 6-Cysteine protein family. Previous studies revealed that 3 of 6-Cysteine family members (P41, P48/45, and P230) could be used as a vaccine candidate. From this information, the evolution properties and the characteristics of these proteins were further analyzed. Protein sequences of 6-Cysteine protein family members were retrieved from plasmoDB and the GenBank. Maximum likelihood phylogenetic tree and time trees were then constructed by using MEGAX, protein domain analysis was done by using InterPro, and all tertiary structures of these proteins were predicted by using PHYRE2. Phylogenetic tree and time tree analysis showed that the human-infecting and the non-infectious Plasmodium have a different cluster and evolutionary rates. Furthermore, several domains that can be used vaccine targets were found in P41, P48/45, and P230, such as transmembrane, signal peptide, and a coiled-coil domain. Tertiary structure prediction also revealed a different characteristic of these proteins. Thus, our findings provide valuable information to support the development of the cross-species antimalaria vaccine using 6-Cysteine protein family members.

Keywords: 6-Cysteine, drug target, protein domain, protein evolution, tertiary structure, Plasmodium
Isolation and Screening of Microbial Isolates from Kombucha Culture for Bacterial Cellulose Production in Sugarcane Molasses Medium
Authors: Angela Clara , Young Jeffrey , Kordayanti Sisilia , Virgina Partha Devanthi Putu , . Katherine 
Published: February 2020

Kombucha tea is a traditional fermented beverage of Manchurian origins which is made of sugar and tea. The fermentation involves the application of a symbiotic consortium of bacteria and yeast (SCOBY) in which their metabolites provide health benefits for the consumer and subsequently allow the product to protect itself from contamination. Additionally, kombucha tea fermentation also produces a byproduct in the form of a pellicle composed of cellulose (Bacterial Cellulose, BC). Compared to plant cellulose, BC properties are more superior, which makes it industrially important. However, BC production at industrial scale has been faced with many challenges, including low yield and high fermentation medium cost. Many researchers have focused their studies on the use of alternative low cost media, such as molasses, which is a by-product of sugar refining process. To maximize the BC production in molasses medium, it is important to select the microbial strains that can grow and produce BC at high yield in molasses. This study aimed to isolate and characterize BC-producing bacteria and a dominant yeast from kombucha culture which had been previously adapted in molasses medium. The isolation of bacteria was performed using Nutrient Agar (NA) and Hestrin and Schramm (HS) supplemented with cycloheximide, while yeast was isolated using Potato Dextrose Agar (PDA) supplemented with chloramphenicol. The most dominant colonies were isolated and then subjected to microscopic observation for morphological analysis. The pure bacteria and yeast isolates were then identified by sequencing the 16S rRNA gene and D1/D2 region of the 26S rRNA, respectively. The bacteria isolates obtained were all from closely related genera: Komagataeibacter sp. DS1MA.62A, Komagataeibacter xylinus, Komagataeibacter saccharivorans, Komagataeibacter xylinus and Gluconacetobacter saccharivorans. The single isolated yeast was identified as Brettanomyces bruxellensis. This study helps to elucidate the BC-producing species which thrive in molasses medium for potential use in the BC production using molasses as alternative cheap carbon source. Also, the study revealed that the co-culture of Komagataeibacter sp. DS1MA.62A and B. bruxellensis could produce BC from molasses supplemented with caffeine and acetate buffer at an average yield of 27.7±1.83 g/L.
Keywords: Kombucha, SCOBY, bacterial cellulose, Acetobacter, Komagataeibacter, Brettanomyces, alternative medium, molasses, caffeine, acetate buffer.