num_pages: 272
description: Leading researchers in the biological, chemical, and physical investigation of superantigens describe in step-by-step detail their best experimental techniques to assess the physical characteristics and biological effects of superantigens. Their protocols range from those for investigating the interactions of superantigens with cellular receptors to those for the analysis of their immunological and biological effects, including methods for using BIOcore to determine binding kinetics and establishing various lymphocyte cell culture systems. There are also accounts of such methods as the RNase protection assay, cytokine ELISA, FACS analysis, and cytokine production at the single cell level.
language: English
title: Methods in Molecular Biology, Volume 214: Superantigen Protocols
titlecomplete: Methods in Molecular Biology, Volume 214: Superantigen Protocols
ratings_count: 1
publisher: Humana